LOCUS       DACDIE010000001       129426 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.001, whole genome shotgun sequence.
ACCESSION   DACDIE010000001 DACDIE010000000
VERSION     DACDIE010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 129426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 129426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..129426
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.001"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            592..1281
                     /locus_tag="IV250_000001"
     CDS             592..1281
                     /locus_tag="IV250_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8767319.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1268..2476
                     /locus_tag="IV250_000002"
     CDS             1268..2476
                     /locus_tag="IV250_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8767320.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2770..4041
                     /gene="serS"
                     /locus_tag="IV250_000003"
     CDS             2770..4041
                     /gene="serS"
                     /locus_tag="IV250_000003"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAP8767321.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4590..6074)
                     /gene="guaB"
                     /locus_tag="IV250_000004"
     CDS             complement(4590..6074)
                     /gene="guaB"
                     /locus_tag="IV250_000004"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAP8767322.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6579..7274)
                     /locus_tag="IV250_000005"
     CDS             complement(6579..7274)
                     /locus_tag="IV250_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HAP8767323.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7364..8464)
                     /gene="ychF"
                     /locus_tag="IV250_000006"
     CDS             complement(7364..8464)
                     /gene="ychF"
                     /locus_tag="IV250_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAP8767324.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8473..8664)
                     /locus_tag="IV250_000007"
     CDS             complement(8473..8664)
                     /locus_tag="IV250_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAP8767325.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8685..9575)
                     /locus_tag="IV250_000008"
     CDS             complement(8685..9575)
                     /locus_tag="IV250_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAP8767326.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9562..10329)
                     /locus_tag="IV250_000009"
     CDS             complement(9562..10329)
                     /locus_tag="IV250_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP8767327.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10341..11054)
                     /gene="rsmG"
                     /locus_tag="IV250_000010"
     CDS             complement(10341..11054)
                     /gene="rsmG"
                     /locus_tag="IV250_000010"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAP8767328.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11286..13187)
                     /gene="mnmG"
                     /locus_tag="IV250_000011"
     CDS             complement(11286..13187)
                     /gene="mnmG"
                     /locus_tag="IV250_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAP8767329.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13204..14601)
                     /gene="mnmE"
                     /locus_tag="IV250_000012"
     CDS             complement(13204..14601)
                     /gene="mnmE"
                     /locus_tag="IV250_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAP8767330.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14945..15283
                     /locus_tag="IV250_000013"
     CDS             14945..15283
                     /locus_tag="IV250_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8767331.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(15451..16209)
                     /locus_tag="IV250_000014"
     CDS             complement(15451..16209)
                     /locus_tag="IV250_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP8767332.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16227..17045)
                     /locus_tag="IV250_000015"
     CDS             complement(16227..17045)
                     /locus_tag="IV250_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP8767333.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17042..17398)
                     /gene="rnpA"
                     /locus_tag="IV250_000016"
     CDS             complement(17042..17398)
                     /gene="rnpA"
                     /locus_tag="IV250_000016"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAP8767334.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17516..17650)
                     /gene="rpmH"
                     /locus_tag="IV250_000017"
     CDS             complement(17516..17650)
                     /gene="rpmH"
                     /locus_tag="IV250_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAP8767335.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18173..19507
                     /gene="dnaA"
                     /locus_tag="IV250_000018"
     CDS             18173..19507
                     /gene="dnaA"
                     /locus_tag="IV250_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAP8767336.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19705..20835
                     /gene="dnaN"
                     /locus_tag="IV250_000019"
     CDS             19705..20835
                     /gene="dnaN"
                     /locus_tag="IV250_000019"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAP8767337.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            21064..21306
                     /gene="yaaA"
                     /locus_tag="IV250_000020"
     CDS             21064..21306
                     /gene="yaaA"
                     /locus_tag="IV250_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAP8767338.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21293..22417
                     /gene="recF"
                     /locus_tag="IV250_000021"
     CDS             21293..22417
                     /gene="recF"
                     /locus_tag="IV250_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAP8767339.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22414..24360
                     /gene="gyrB"
                     /locus_tag="IV250_000022"
     CDS             22414..24360
                     /gene="gyrB"
                     /locus_tag="IV250_000022"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAP8767340.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24380..26851
                     /gene="gyrA"
                     /locus_tag="IV250_000023"
     CDS             24380..26851
                     /gene="gyrA"
                     /locus_tag="IV250_000023"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAP8767341.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            27087..27386
                     /locus_tag="IV250_000024"
     CDS             27087..27386
                     /locus_tag="IV250_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAP8767342.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27436..27948
                     /gene="ssb"
                     /locus_tag="IV250_000025"
     CDS             27436..27948
                     /gene="ssb"
                     /locus_tag="IV250_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP8767343.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27974..28210
                     /gene="rpsR"
                     /locus_tag="IV250_000026"
     CDS             27974..28210
                     /gene="rpsR"
                     /locus_tag="IV250_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAP8767344.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28353..30326
                     /locus_tag="IV250_000027"
     CDS             28353..30326
                     /locus_tag="IV250_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HAP8767345.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30332..30784
                     /gene="rplI"
                     /locus_tag="IV250_000028"
     CDS             30332..30784
                     /gene="rplI"
                     /locus_tag="IV250_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAP8767346.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            31058..32425
                     /gene="dnaB"
                     /locus_tag="IV250_000029"
     CDS             31058..32425
                     /gene="dnaB"
                     /locus_tag="IV250_000029"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAP8767347.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33205..34497
                     /locus_tag="IV250_000030"
     CDS             33205..34497
                     /locus_tag="IV250_000030"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAP8767348.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
     gene            35284..36147
                     /locus_tag="IV250_000031"
     CDS             35284..36147
                     /locus_tag="IV250_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP8767349.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(36276..37898)
                     /locus_tag="IV250_000032"
     CDS             complement(36276..37898)
                     /locus_tag="IV250_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8767350.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            38147..38668
                     /locus_tag="IV250_000033"
     CDS             38147..38668
                     /locus_tag="IV250_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8767351.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(38854..40584)
                     /gene="spxB"
                     /locus_tag="IV250_000034"
     CDS             complement(38854..40584)
                     /gene="spxB"
                     /locus_tag="IV250_000034"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HAP8767352.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            40749..41567
                     /locus_tag="IV250_000035"
     CDS             40749..41567
                     /locus_tag="IV250_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAP8767353.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(41670..41867)
                     /locus_tag="IV250_000036"
     CDS             complement(41670..41867)
                     /locus_tag="IV250_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8767354.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            42036..42614
                     /locus_tag="IV250_000037"
     CDS             42036..42614
                     /locus_tag="IV250_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP8767355.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            complement(43659..44162)
                     /locus_tag="IV250_000038"
     CDS             complement(43659..44162)
                     /locus_tag="IV250_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP8767356.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            44521..45546
                     /locus_tag="IV250_000039"
     CDS             44521..45546
                     /locus_tag="IV250_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP8767357.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNLMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            45869..46747
                     /locus_tag="IV250_000040"
     CDS             45869..46747
                     /locus_tag="IV250_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8767358.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            47223..47339
                     /locus_tag="IV250_000041"
     CDS             47223..47339
                     /locus_tag="IV250_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP8767359.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            48267..48605
                     /locus_tag="IV250_000042"
     CDS             48267..48605
                     /locus_tag="IV250_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8767360.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            48699..48929
                     /locus_tag="IV250_000043"
     CDS             48699..48929
                     /locus_tag="IV250_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8767361.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            49329..49700
                     /locus_tag="IV250_000044"
     CDS             49329..49700
                     /locus_tag="IV250_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAP8767362.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(49974..50666)
                     /locus_tag="IV250_000045"
     CDS             complement(49974..50666)
                     /locus_tag="IV250_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP8767363.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(50669..51454)
                     /locus_tag="IV250_000046"
     CDS             complement(50669..51454)
                     /locus_tag="IV250_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP8767364.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            51691..53091
                     /gene="lacG"
                     /locus_tag="IV250_000047"
     CDS             51691..53091
                     /gene="lacG"
                     /locus_tag="IV250_000047"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAP8767365.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            53116..53433
                     /locus_tag="IV250_000048"
     CDS             53116..53433
                     /locus_tag="IV250_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP8767366.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            53446..55119
                     /locus_tag="IV250_000049"
     CDS             53446..55119
                     /locus_tag="IV250_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8767367.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            55133..56764
                     /locus_tag="IV250_000050"
     CDS             55133..56764
                     /locus_tag="IV250_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HAP8767368.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(57255..58820)
                     /gene="guaA"
                     /locus_tag="IV250_000051"
     CDS             complement(57255..58820)
                     /gene="guaA"
                     /locus_tag="IV250_000051"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAP8767369.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(58879..59802)
                     /locus_tag="IV250_000052"
     CDS             complement(58879..59802)
                     /locus_tag="IV250_000052"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAP8767370.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            59998..60603
                     /locus_tag="IV250_000053"
     CDS             59998..60603
                     /locus_tag="IV250_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8767371.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            60911..61927
                     /locus_tag="IV250_000054"
     CDS             60911..61927
                     /locus_tag="IV250_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8767372.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            62068..63369
                     /locus_tag="IV250_000055"
     CDS             62068..63369
                     /locus_tag="IV250_000055"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAP8767373.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            63415..64077
                     /gene="deoC"
                     /locus_tag="IV250_000056"
     CDS             63415..64077
                     /gene="deoC"
                     /locus_tag="IV250_000056"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAP8767374.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            64098..64496
                     /locus_tag="IV250_000057"
     CDS             64098..64496
                     /locus_tag="IV250_000057"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAP8767375.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            64562..65644
                     /locus_tag="IV250_000058"
     CDS             64562..65644
                     /locus_tag="IV250_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8767376.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            65856..67418
                     /locus_tag="IV250_000059"
     CDS             65856..67418
                     /locus_tag="IV250_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8767377.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            67411..68544
                     /locus_tag="IV250_000060"
     CDS             67411..68544
                     /locus_tag="IV250_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8767378.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            68544..69500
                     /locus_tag="IV250_000061"
     CDS             68544..69500
                     /locus_tag="IV250_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8767379.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            69599..70765
                     /gene="deoB"
                     /locus_tag="IV250_000062"
     CDS             69599..70765
                     /gene="deoB"
                     /locus_tag="IV250_000062"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAP8767380.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            70789..71607
                     /locus_tag="IV250_000063"
     CDS             70789..71607
                     /locus_tag="IV250_000063"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP8767381.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            71624..72331
                     /gene="deoD"
                     /locus_tag="IV250_000064"
     CDS             71624..72331
                     /gene="deoD"
                     /locus_tag="IV250_000064"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP8767382.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(72629..73315)
                     /gene="gpmA"
                     /locus_tag="IV250_000065"
     CDS             complement(72629..73315)
                     /gene="gpmA"
                     /locus_tag="IV250_000065"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP8767383.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(73487..74068)
                     /locus_tag="IV250_000066"
     CDS             complement(73487..74068)
                     /locus_tag="IV250_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8767384.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(74201..74884)
                     /gene="rpiA"
                     /locus_tag="IV250_000067"
     CDS             complement(74201..74884)
                     /gene="rpiA"
                     /locus_tag="IV250_000067"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAP8767385.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            75203..75616
                     /gene="rpsL"
                     /locus_tag="IV250_000068"
     CDS             75203..75616
                     /gene="rpsL"
                     /locus_tag="IV250_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAP8767386.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            75700..76170
                     /gene="rpsG"
                     /locus_tag="IV250_000069"
     CDS             75700..76170
                     /gene="rpsG"
                     /locus_tag="IV250_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAP8767387.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            76264..78351
                     /gene="fusA"
                     /locus_tag="IV250_000070"
     CDS             76264..78351
                     /gene="fusA"
                     /locus_tag="IV250_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP8767388.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            78523..79710
                     /gene="tuf"
                     /locus_tag="IV250_000071"
     CDS             78523..79710
                     /gene="tuf"
                     /locus_tag="IV250_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP8767389.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            80117..80425
                     /gene="rpsJ"
                     /locus_tag="IV250_000072"
     CDS             80117..80425
                     /gene="rpsJ"
                     /locus_tag="IV250_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAP8767390.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            80456..81085
                     /gene="rplC"
                     /locus_tag="IV250_000073"
     CDS             80456..81085
                     /gene="rplC"
                     /locus_tag="IV250_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAP8767391.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            81113..81736
                     /gene="rplD"
                     /locus_tag="IV250_000074"
     CDS             81113..81736
                     /gene="rplD"
                     /locus_tag="IV250_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAP8767392.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            81736..82026
                     /gene="rplW"
                     /locus_tag="IV250_000075"
     CDS             81736..82026
                     /gene="rplW"
                     /locus_tag="IV250_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAP8767393.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            82065..82898
                     /gene="rplB"
                     /locus_tag="IV250_000076"
     CDS             82065..82898
                     /gene="rplB"
                     /locus_tag="IV250_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAP8767394.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNRAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            82941..83219
                     /gene="rpsS"
                     /locus_tag="IV250_000077"
     CDS             82941..83219
                     /gene="rpsS"
                     /locus_tag="IV250_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAP8767395.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            83242..83589
                     /gene="rplV"
                     /locus_tag="IV250_000078"
     CDS             83242..83589
                     /gene="rplV"
                     /locus_tag="IV250_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAP8767396.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            83603..84259
                     /gene="rpsC"
                     /locus_tag="IV250_000079"
     CDS             83603..84259
                     /gene="rpsC"
                     /locus_tag="IV250_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAP8767397.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            84262..84696
                     /gene="rplP"
                     /locus_tag="IV250_000080"
     CDS             84262..84696
                     /gene="rplP"
                     /locus_tag="IV250_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAP8767398.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            84686..84874
                     /gene="rpmC"
                     /locus_tag="IV250_000081"
     CDS             84686..84874
                     /gene="rpmC"
                     /locus_tag="IV250_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAP8767399.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            84899..85165
                     /gene="rpsQ"
                     /locus_tag="IV250_000082"
     CDS             84899..85165
                     /gene="rpsQ"
                     /locus_tag="IV250_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAP8767400.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            85223..85591
                     /gene="rplN"
                     /locus_tag="IV250_000083"
     CDS             85223..85591
                     /gene="rplN"
                     /locus_tag="IV250_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAP8767401.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            85628..85936
                     /gene="rplX"
                     /locus_tag="IV250_000084"
     CDS             85628..85936
                     /gene="rplX"
                     /locus_tag="IV250_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAP8767402.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            85964..86503
                     /gene="rplE"
                     /locus_tag="IV250_000085"
     CDS             85964..86503
                     /gene="rplE"
                     /locus_tag="IV250_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAP8767403.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            86522..86707
                     /locus_tag="IV250_000086"
     CDS             86522..86707
                     /locus_tag="IV250_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAP8767404.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            86743..87141
                     /gene="rpsH"
                     /locus_tag="IV250_000087"
     CDS             86743..87141
                     /gene="rpsH"
                     /locus_tag="IV250_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAP8767405.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            87173..87709
                     /gene="rplF"
                     /locus_tag="IV250_000088"
     CDS             87173..87709
                     /gene="rplF"
                     /locus_tag="IV250_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAP8767406.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            87870..88226
                     /gene="rplR"
                     /locus_tag="IV250_000089"
     CDS             87870..88226
                     /gene="rplR"
                     /locus_tag="IV250_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAP8767407.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            88247..88747
                     /gene="rpsE"
                     /locus_tag="IV250_000090"
     CDS             88247..88747
                     /gene="rpsE"
                     /locus_tag="IV250_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAP8767408.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            88762..88941
                     /gene="rpmD"
                     /locus_tag="IV250_000091"
     CDS             88762..88941
                     /gene="rpmD"
                     /locus_tag="IV250_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAP8767409.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            88985..89425
                     /gene="rplO"
                     /locus_tag="IV250_000092"
     CDS             88985..89425
                     /gene="rplO"
                     /locus_tag="IV250_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAP8767410.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            89425..90720
                     /gene="secY"
                     /locus_tag="IV250_000093"
     CDS             89425..90720
                     /gene="secY"
                     /locus_tag="IV250_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAP8767411.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            90782..91429
                     /locus_tag="IV250_000094"
     CDS             90782..91429
                     /locus_tag="IV250_000094"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAP8767412.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            91620..91838
                     /gene="infA"
                     /locus_tag="IV250_000095"
     CDS             91620..91838
                     /gene="infA"
                     /locus_tag="IV250_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAP8767413.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            91871..91987
                     /gene="rpmJ"
                     /locus_tag="IV250_000096"
     CDS             91871..91987
                     /gene="rpmJ"
                     /locus_tag="IV250_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAP8767414.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            92005..92370
                     /gene="rpsM"
                     /locus_tag="IV250_000097"
     CDS             92005..92370
                     /gene="rpsM"
                     /locus_tag="IV250_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAP8767415.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            92398..92787
                     /gene="rpsK"
                     /locus_tag="IV250_000098"
     CDS             92398..92787
                     /gene="rpsK"
                     /locus_tag="IV250_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAP8767416.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            92867..93805
                     /locus_tag="IV250_000099"
     CDS             92867..93805
                     /locus_tag="IV250_000099"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAP8767417.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            93833..94213
                     /gene="rplQ"
                     /locus_tag="IV250_000100"
     CDS             93833..94213
                     /gene="rplQ"
                     /locus_tag="IV250_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAP8767418.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            94485..95324
                     /locus_tag="IV250_000101"
     CDS             94485..95324
                     /locus_tag="IV250_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP8767419.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            95300..96169
                     /locus_tag="IV250_000102"
     CDS             95300..96169
                     /locus_tag="IV250_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP8767420.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            96159..96956
                     /locus_tag="IV250_000103"
     CDS             96159..96956
                     /locus_tag="IV250_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP8767421.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            97159..97905
                     /gene="truA"
                     /locus_tag="IV250_000104"
     CDS             97159..97905
                     /gene="truA"
                     /locus_tag="IV250_000104"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP8767422.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(98360..101029)
                     /locus_tag="IV250_000105"
     CDS             complement(98360..101029)
                     /locus_tag="IV250_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP8767423.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFFYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(101267..101455)
                     /locus_tag="IV250_000106"
     CDS             complement(101267..101455)
                     /locus_tag="IV250_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAP8767424.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            101725..102087
                     /locus_tag="IV250_000107"
     CDS             101725..102087
                     /locus_tag="IV250_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP8767425.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            102139..103821
                     /locus_tag="IV250_000108"
     CDS             102139..103821
                     /locus_tag="IV250_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAP8767426.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            103938..105974
                     /gene="recG"
                     /locus_tag="IV250_000109"
     CDS             103938..105974
                     /gene="recG"
                     /locus_tag="IV250_000109"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAP8767427.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            106023..107024
                     /gene="plsX"
                     /locus_tag="IV250_000110"
     CDS             106023..107024
                     /gene="plsX"
                     /locus_tag="IV250_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAP8767428.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            107146..107388
                     /gene="acpP"
                     /locus_tag="IV250_000111"
     CDS             107146..107388
                     /gene="acpP"
                     /locus_tag="IV250_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP8767429.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            107656..108660
                     /locus_tag="IV250_000112"
     CDS             107656..108660
                     /locus_tag="IV250_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8767430.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            108661..109605
                     /locus_tag="IV250_000113"
     CDS             108661..109605
                     /locus_tag="IV250_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8767431.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            109605..110567
                     /locus_tag="IV250_000114"
     CDS             109605..110567
                     /locus_tag="IV250_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8767432.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            110594..111508
                     /locus_tag="IV250_000115"
     CDS             110594..111508
                     /locus_tag="IV250_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8767433.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            111534..113315
                     /locus_tag="IV250_000116"
     CDS             111534..113315
                     /locus_tag="IV250_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8767434.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            113663..114349
                     /locus_tag="IV250_000117"
     CDS             113663..114349
                     /locus_tag="IV250_000117"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP8767435.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            114369..117950
                     /gene="smc"
                     /locus_tag="IV250_000118"
     CDS             114369..117950
                     /gene="smc"
                     /locus_tag="IV250_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAP8767436.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            117959..118768
                     /locus_tag="IV250_000119"
     CDS             117959..118768
                     /locus_tag="IV250_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8767437.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            118779..119777
                     /gene="ftsY"
                     /locus_tag="IV250_000120"
     CDS             118779..119777
                     /gene="ftsY"
                     /locus_tag="IV250_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAP8767438.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            119964..122231
                     /gene="gshAB"
                     /locus_tag="IV250_000121"
     CDS             119964..122231
                     /gene="gshAB"
                     /locus_tag="IV250_000121"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HAP8767439.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(122320..122772)
                     /locus_tag="IV250_000122"
     CDS             complement(122320..122772)
                     /locus_tag="IV250_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HAP8767440.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            122922..123872
                     /locus_tag="IV250_000123"
     CDS             122922..123872
                     /locus_tag="IV250_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP8767441.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            123865..124824
                     /locus_tag="IV250_000124"
     CDS             123865..124824
                     /locus_tag="IV250_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP8767442.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            124821..125576
                     /locus_tag="IV250_000125"
     CDS             124821..125576
                     /locus_tag="IV250_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8767443.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHIGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            125599..126552
                     /locus_tag="IV250_000126"
     CDS             125599..126552
                     /locus_tag="IV250_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8767444.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(127196..127492)
                     /locus_tag="IV250_000127"
     CDS             complement(127196..127492)
                     /locus_tag="IV250_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HAP8767445.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            127847..128203
                     /locus_tag="IV250_000128"
     CDS             127847..128203
                     /locus_tag="IV250_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP8767446.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            128213..129112
                     /locus_tag="IV250_000129"
     CDS             128213..129112
                     /locus_tag="IV250_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HAP8767447.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
BASE COUNT        41028 a        23659 c        27034 g        37705 t
ORIGIN      
        1 aggagtgttt cccgattcgt ttttggctgc cgtagcaact tttatatcat atcagagaga
       61 caagtaattg tcaactcttt tttaaaagtt ttttcgattt cgtttttcga aatcttccag
      121 caactcattt atcataactt atcgtttgtt atttgtcaag aacttttttg aagttttttt
      181 cgatagaatc aagcttcgca agtggttctt ttttaacgac tcagttatct tatcatgtca
      241 tttccaacat gtcaacaact tattttttat tatttaataa gttattggtg ttcttttgaa
      301 caaataataa tatatcaaca tcacatgtga aagtcaataa gaaaatgctt atttatcaac
      361 atttctagct tacatgatca ttggttagtt ctggtccttt ttcgttcgtc tttataactc
      421 cgagagacgt aaaatccttt tgttttcagt ttaatgttca tgttcttgtc ttattcacac
      481 caactagatg tctcaccttc taaaattttc cttaacaaag tgctgctttt atctatttct
      541 gctttttttt gctagaataa tagtaacaat atgactctgg aggatttatt tatgaaaatt
      601 ttgattgctg acgatgataa agaaatcgtt gaattattaa gtatctatgt tcacaatgag
      661 gggtatgaag cagtaaaggc ttatgatggg aaagaagcat tatctaaaat cagaacaatc
      721 cctgatattg aattacttat tttagatatc atgatgcctg agatggatgg gatgcaagta
      781 gtgaaagagc tacgaaaaga atcccaaata ccaatcatta tgctgacagc aaaaactact
      841 gatatggata agatcaaagg actggtcgct ggtgcagatg attatgtgac caagccattt
      901 aatccgttag aagtaatggc tagagtaaag tctttattac ggcgtacaaa aatgcagatt
      961 gccccagatc agcctgatgt attagaagta aatgcattaa tcatcaataa agactctcat
     1021 gaagttaaaa caatcgatgg gaaagaaatc cagttaacag ctttggaatt cggtatcctc
     1081 tacttgcttg ctagtcatcc taatcgagta tttagcgcgg atgaaatttt tgagcgtgtt
     1141 tggaaacaag aaagtattgt ctctgctaaa actgtcatgg ttcacgttag tcatttaaga
     1201 gataagattg aagaagcaac tggtggtgaa aaagtcattc aaaccgtttg gggagtgggc
     1261 tataaaattg acgcaagata gtaaacgagc aactgaaaaa cgccgtcgga tcaatttgac
     1321 ttcaaaagaa atcagcgaat tgttagcgga aggaatcatc acgattatcc ttttgcttct
     1381 attaaatgtt tctattctgg ttgttatcag ttcggtgatc aacagttcac cttctttgac
     1441 gaatgcgatt tgggattcaa agaatatttt tgcagaacga ttgaacactg atttgttctg
     1501 gaacggccgg aattttatta ttcccttttt ctttctatta gatattggtg ttttgtattg
     1561 gcgcttgatt cgaagatatc gccaaatgca attgcggcat atcatcagcg aacttcacta
     1621 catcgcaaac ggaaattatg atcatcgtat cccttttgaa ctgagtggtg acttaagccg
     1681 agtggtaaca agtatcaatg gattggtgga tagtaccgta gcagcgattg aagacgaacg
     1741 taaaatcgaa aaatcaaaag atgaattgat cacgaatgtc agtcatgata tccgtacccc
     1801 actcacgtcc atcattggat acctaggact gatagaagat ggccagtatc actcagaaga
     1861 agatctttta aagtatacgc atactgctta tatcaaagca aagcagatga aatctttagt
     1921 ggacgactta tttgaataca ctaaggtacg ccagcctgct gtcccggtga atttttctgc
     1981 ctttgacatg atccagttga tcgaacagtt agcagctgat tttgagttgg aagcttctaa
     2041 aaaaaatatc caaatacttg ttcaatcaaa agtagactcc cttatcatgg atggcgatac
     2101 cgaaaaactt gtacgtgtct tcaataatct tttgacgaat gcgttgaaat acggaaaagg
     2161 agcaacgaag atcgtaatcg aagtagaacg tatcggttca gaagttgttg caacagtcaa
     2221 aaataacgga gcaatgatcc cacaacaggc aatcgataat ctattcgacc gtttttatcg
     2281 tgtagaagaa tctcgttcac aagcaactgg aggaactgga cttggtttag caatcgctca
     2341 aagcattgtg gctttgcacg gcggatacat ttatgcaaaa tcagataagc aatggacttc
     2401 tttcatcatg caccttccaa tcaagaaaaa tgaaaaatta cctattgata cacaggaagt
     2461 tattgacgaa agctgaaata ttcgctattc ttattacaat tattagaata aatgaaaaac
     2521 acagaaccca gtacacttta ttctttttca gagagctgat ggcaggtgta aatcagcaaa
     2581 ggataatttg gaagttccag ttcgtttacg tgccctttcg aaataagaaa ggtcggttca
     2641 acagccgtta tgtgttcaac aagtgcagct gacgctgaac ttgggtggta ccgcgaattt
     2701 cagactgatt cgtcccaaga ataatgggat ggatcagtct ttttttattt tcaaaaagga
     2761 ggaaatcata tgttagatgt aaaaatgatg cgacaaaata ttgaagaagt acaagaaaaa
     2821 ttgatgactc ggggcgtaaa aaaagaaaca ttagctgaat tcatggaact agatgaaaat
     2881 cgtcgtaagc tactagtcaa aagtgaagaa ttaaaaaaat atcgaaatga tgtttctgct
     2941 gagatcgccc aattgaagcg tgcaaaagaa gatgcttctg ctaaaatcgc tgaaatgaaa
     3001 gaagtcggtg gcaacatcaa agcattggat actgaaattg ctgaagtgga tgaaaaattg
     3061 aaacaaattg caactacttt gccaaacctt ccccatgatt ctgtgccagt tggaacagat
     3121 gaagaggaca atgtagaaat ccgtcgttgg agcgaaccac gtacatttgc ttttgaacca
     3181 aaaccacact gggaaattgc agaaaatctt gatattttag attttgaacg tggcgcaaaa
     3241 gtagccggta gccgttttgt ctattacaaa ggattaggtg ctcgattaga acgtgctgtc
     3301 tacaacttca tgcttgatca gcatatttac gaacacggct atactgaaat gatcactcct
     3361 tatatcgtca acagtaaagc gatgtttggt acgggacaat ttccaaaatt caaagaagat
     3421 gttttccaat tggagaatac tgatctaaca ttaatcccaa cagctgaagt accattaact
     3481 aactattata atgatgaaat tttagatggt gcagatttac ctatttattt cactgcacta
     3541 agccctgctt ttcgttctga agccggaagt gctggtcggg atacacgagg actgattcgt
     3601 cttcaccaat tcaataaagt agaaatggtc aaattttcgg atgtagagca ttcttacgaa
     3661 gaattggaaa aaatgacagc tgatgcagaa gatatcttga aaaaattgca tttgccttat
     3721 cgtgtgatgg ccttatcaac tggagacatg ggcttttctg cagctaaaac ttatgactta
     3781 gaagtatgga tacctgcaca agatacttat cgtgaaatca gttcttgttc taactgtgaa
     3841 gatttccaag cacgccgcgc aatgatccgt tatcgcgata atgatggaaa aatccactat
     3901 actcatactc tgaatggttc aggacttgca gttggccgta cagtagctgc cattttggaa
     3961 aactaccaaa atgaagatgg ttctgtcaca gttccagaag cgctcgttcc ttatatgggg
     4021 aatctgaaag ttattaaata aagaggctgt gaaaaagccg cttagtttcg agtattaaga
     4081 aaaattttgt gaaaatggct ttccacattt ttgcataaat tggacttaat accgagaaac
     4141 tggcttttga acaccattta tcgagaggtt gtgaaatcag cgaattaacc tgagtattag
     4201 agaaaaaaat ggaaaaatag tccctcatat tttaccgttt gttgaactaa taccgagggt
     4261 cagctgattg aacaccgttt agtggagagg tcgtaagcct accccccagc tcctagttac
     4321 aaggacaaat tcaaaaaaca gctcttcata ttttatgaat gttgtacttg tgattcggag
     4381 gtggcaaggg aacactgttt attcaaaaag aggctgggac agaagcgttt aactccaaga
     4441 aataagaaga aattcacgaa aattgctttt caaatttttg tgaatttctt cttatttccg
     4501 aaggagttgc ttctacttcc gccgtttata cgtgtttaga gcgtgagaca aaagtgttct
     4561 ttacttttgt cccacgctct ttaatgtttt taagattcaa ctgaatagtt tggtgcttct
     4621 tttgtgattt gtacatcatg agggtgtgat tcttttaatc cactgccgct catttggata
     4681 aattgcgctt catcacgtaa ttgctgtaga ttggctgccc caacataacc cattcctgaa
     4741 cgcaaaccgc cgatcatttg gaaaataata tctgcaacac ttcctttata agcaacccga
     4801 ccttcgattc cttctggtac taatttattt gcttcattga cactaccttg gaagtaacgg
     4861 tcttttgaac ctttttccat cgcgcctaat gagcccatac ctcggtatgt tttgaatcga
     4921 cgtccttgga agatttcgaa ttcacctggt gattcatctg tccctgccaa catacttcct
     4981 agcatcactg catgtccgcc ggcagctaat gctttgacga tgtcaccaga atatttgatc
     5041 cctccgtctg caataatcgc tttgccatat tgacgagcaa ctgatgctgc atcataaata
     5101 gctgtgagct gaggtacacc tacaccggct accacacgtg tcgtacaaat cgatcctggt
     5161 ccgataccaa cttttacgac atctacacct gcatcatata aagcttttgt cgcttcagcc
     5221 gtcgcaacgt ttcctgcaat caatgttgca tctgcaaaag ttgaacggat ttcttgaatt
     5281 tttcggatca cacctgcact atgtccatga gctgtatcga tgatgattgc atctgcccct
     5341 gcttctaata aagcattcgc acgctcaaat gtatcgcttg ttacaccaac ggctgctgct
     5401 acaagcaatc gaccatgttc atcttttgct gcatttggaa attcgatcac tttctcgatg
     5461 tctttgatcg tgatcaatcc gcttaaacga ccttcgttat ctacgattgg taatttttcg
     5521 attttatgtt tttgcaaaat cttttctgca tcttttagag aggtacctac tggggcagtc
     5581 actaagttgt cttttgtcat cacatcagca atcggcatgt gataatctgt tacaaaacgc
     5641 atgtcgcggt ttgtaattat ccctaccaat tttctgtttt caagtgtttc aacgattggc
     5701 acaccactga tacgatattt gctcatcaat tcttctgcgt ctgctacaag atgctgtggc
     5761 gttaagaaga acggatcaat gattacgcca ctttcagaac gtttgacttt tcttacttca
     5821 tccgcttgct ggctgatcgt catgttttta tggatcacac ctagtccgcc ttgtcttgcc
     5881 atagcaatgg ccatcttgct gtcggtaaca gtatccatac ttgcactgat gattggaata
     5941 ttcaacttaa tatttttagc taattgtacg ctcatatcca cttcgtttgg taacacgtga
     6001 ctttctgctg gtatcaataa gacatcatca aaagtaaatc cttttttcgt gaactttgtt
     6061 tcccagttgg acatttttag taccactcct ctaatttagt ttatctatta aaataattga
     6121 ttttttcatt taagtcaatg atctacttgg aatcaagtta gctttctcat agaataccaa
     6181 tataaagcgt ttacatctgt gtgtattaaa agagattgta aaaaagctgt cctgcatcaa
     6241 gtaataagaa cagccaatca aaaacagctt ctcatgtttt tgaattggtt gggtttatta
     6301 ccgcagatgc aaacttttga acaccgttta gtggagaggc tgtgaaaaag ctgcttagtt
     6361 tcgagtagta agaaaaattt tgtgaaaatg gctttccaca tttttgcata aattggactt
     6421 aataccgaga aactggcttt tgaacaccgt ttatcgtaaa aagggactgt gacaagactt
     6481 ttgtcacagt cccttactat tatttaacta tggataaaca aaatgaggtt ctttactacc
     6541 cctagatatc tagcaaaata gctgatcttt ttgctagttt atcttcttgg gacggataaa
     6601 cttccaataa tcccgtattt tttcttcaag tagtaacgga ttgctaaagc agttgccccg
     6661 ataatgataa ctactactgg atctaatact ggattgataa ctggtggcaa tagtgttgtc
     6721 gcagaaaaca gtacgaccca aagaagtgtc gttccaccta ggataagcaa tgtcttaccc
     6781 attcctggtt tcttgcttcg atctgctccg ggatattcat attgatagat gtatttgtac
     6841 atcatataaa agacccagcc accgattgca gaacctagaa cgagtgtaga aaagccataa
     6901 gctggcgttg ttccacgtga aaacaatccg gtaatcgcaa tcatgatact taatagacca
     6961 agcaacagca acgtattatc taaccacatc aataaagggg tggatgaatt ttttacttct
     7021 gctggcttat tgatgattgc ttccgttctt tcagagactg tcccaaacaa ttgacgggct
     7081 gttcttccgc ctttttgttc tgcaactaaa gtaggcagaa tgtcatgaag ggcttttact
     7141 ttttcttctt cagacaaatt cgctgcttct aaagattttt tcaaatcaaa aatgtactgt
     7201 tgatttcttt tggttaattt tgtttctaaa ctattgtttt cttttacgat tgctcgtaat
     7261 tgttctggtt ccattatcta atagtcctcc ttagccgttc cttttctcaa cggtcgtcac
     7321 aaaagacatt ctaccatatt ctacagctag tttgatagcc cttttacaca ttaaagcgga
     7381 ataacattac gtcgccgtct tgtacgacat aatcttttcc ttctaatcga acacgtccag
     7441 cttcttttgc tgcatgcatg tttccgtaat gatccaagtc ttcaaaagag acagtttctg
     7501 cacggataaa tccgcgttcg aaatctgaat ggatgattcc tgctgcttgt ggtgctttga
     7561 ttcctttttt gaatgtccaa gcacgtactt cttgttcccc agcagtaaag taagtagcta
     7621 agcctaacaa atcataagca gcacggatca actgatccaa acctgattct tcgattccta
     7681 aagcttctaa aaattccgct ttgtcttctt catctaattc tgcgatttct tcttctgcac
     7741 gagcactgac cacgatcact tctgcatttt cttctgcagc aaagttgcgc acttcttgta
     7801 cataatgatt cgcgtctgca tctgctacgt catcttctga gacattggct acataaagaa
     7861 ttggttttgt cgtcaataaa aatagtgatt taacgatttt ttgttcttct tcagtaaatt
     7921 cgatcgttcg ggcagatttt ccttcttcca gtacaggttt gattttatcc agtacagcta
     7981 gttctgccac tgcatcttta tctttggttt tagcaacttt tgctacacga gtatagcgtt
     8041 tgttgataga ttctaagtct gctaacacta gttccaagtt gatcgtatca atatcttcta
     8101 gtggatcaac acgtccttct acgtgagtaa tattatcatc atcaaaacag cgaacaacgt
     8161 gacaaatagc gtctacttgg cggatatggc ttaagaattg atttcccaaa ccttctcctt
     8221 tacttgcgcc ttttacgatt ccagcaatat ctgtaaattc aaaagtcgtc ggaacagttt
     8281 ttttcggatg tacaagttct gttaagcgtt gcaaacgcca gtcaggtact tctaccatcc
     8341 ctacgttggg gtcgatcgtc gcaaagggat agtttgctgc ttctgctcct gcttttgtga
     8401 tcgcattaaa caaagttgat tttcctacgt tgggcaaccc aacaattcca gcagtcaatg
     8461 ccataaattt attcactctt tctctgcatt tttttcaatt acttttttta atttcttttc
     8521 aaaatctcga cgggtcatca tgacgatatg tccgcaattc atacatctga ttttgatatc
     8581 tgcgcccatt cgaatgactt cccagcgatt cgtctggcaa gcgtgaggtt ttttcatttc
     8641 aacgatatcg tgcaagtcgt acataaaaat agcctccttt ttgtttattc ttcatcaaat
     8701 tgaatattca agatatcaag aattcgagtc aaatcttttt gagaaagata ttcaatctcg
     8761 atcttacctt tcccattttt ttcttggata gctacacttg taccaaattt atccatcaaa
     8821 cgatcttcgc tttctcggat ataatatggt ttttcctttg ctagccttgg gatttttttc
     8881 ttttcttttc catcattttg atttaactca gaaacaagct gttccagttg gcggacagtt
     8941 aaattttcag ttactgcacg attggctaat ttcaagatca atgttttatt ttttaaacct
     9001 aacagcgtac gggcttgtcc catagataaa cgctgatctt gtaccatttc cttgacaaga
     9061 tccggtaaag aaagcaagcg caaataattg gcgatataag gtctgctctt tcctaaacga
     9121 ctagctactt ctgcttgtgt tagtttcagg tttttcatca gcatctcata tgcttctgct
     9181 tcttctaacg gattcagatc ttctcgctgc aagttttcca gcactgctac ttgcatcatc
     9241 gcttcttcgt caaagtctct aacgatagca gggatcgttt ctttttcagc tagtttggac
     9301 gctcgaaaac gtcgctcccc tgcaataatc tcatatcctt ttactgcgga acggcgtacg
     9361 atgatgggtt gaaagacacc tgattgttca atagaattag ctagttcctg caaagatact
     9421 tcttcaaatg ttttacgggg ctgatacgga tttggtcgca gctcattcaa tggtagctgg
     9481 acaacggtat catcttttac atccaccgtc tctaagtttt ctaaatcttg aaacagtgca
     9541 tcgattcctc ggcctaatcc tttacttttc ttcacgactt accacttcct ttgccaatgc
     9601 ttggtagact tcagctcctc gagaacgtgg atcgtagtcg atgatcggtt ttccatgact
     9661 aggtgcttct gataaacgaa tatttcttgg aatgatcgtt tcgtaaactt tttcgcggaa
     9721 gtatttacgt acttcttcta cgacttcatt tcccagattt gttcgtgcat catacattgt
     9781 cagtaagact ccctcgatct ctaattctgg attaaagtgt ttttggacta aacgaaccgt
     9841 attcagtaat tggcttaatc cttccaatgc gtaatactca cactgaacag gtatcaaaat
     9901 cgaatcactt gctgtaaatg aattgattgt cagatggcca agagaaggcg ggcaatcgat
     9961 caaaatataa tcatactgac tgcttacttc tgctaaagat ccttttaatc ttgattctct
    10021 tgccatcatt gacgtcaatt cgatctctgc tcctgctaat tggagagtcg caggcacaat
    10081 actcaaattt tcgtgttcag tgatcaaagt tgcctcatca attggcaatt cattgactag
    10141 aacatcatag atgtctcttg tcacatcagg ttttctgatc ccgacaccac tggtagcatt
    10201 tccttgtgca tccatatcca ctagcaatac ttttttgcca aggctagcaa gacaagcacc
    10261 tagattcacc gtcgtcgttg ttttgccgac gccacctttt tggttcgcaa cagatattat
    10321 ttgtgccatc tcttctcctc ctatttgatt ggctgcttat taggcagtcc gggctttctt
    10381 gggtatttct ttggggttgg tttctttttt tgaataacga tgatatgccg ttgatcagag
    10441 acattcggca aggtaaccgc ctcttctttg aggaatttac cacctaataa agcaatcgct
    10501 tgttttgctt ctgtcaattc ctcttcactt tttgctgctt tcaatgcata aaaatagcct
    10561 tcttgtttag ccaagggcaa acacaattcc gctaacacat tcaaacgagc aaccgcacga
    10621 gcagtgacaa ggtcaaatga tgcacgaaag tgaggatttt gcccaaatgt ttctgcccgg
    10681 tcatgatata aggaagcatc gattcctaat aatcgggtta actcagataa gaaggttatt
    10741 cgtttattca gagaatcaac gatcgttatc tgcagttctg gaaaggcaat ttttaaaggt
    10801 atgctaggaa accctgcccc cgaaccaaca tcgcataaag ataaaggttg cgtaaagtct
    10861 acttcaaatg ctaatgtaag tgaatcgtaa aaatgcttca gatatacgtc tttttcatcc
    10921 gtaatagctg taaggttcat cttttcgttc cattcgacta atgtccgata ataaatagcg
    10981 aattgattca tttgatgatc ggtaagctct atcccttttt ctgccaagga ttggcgaaat
    11041 tcttctggta tcataaatat tcctttcatt gtgaaacagc aattgtttca cagattctct
    11101 ccctacttta acgcaaaatc tctagacatg caatccttcc atatggattc ttaaaaattt
    11161 gaaaagtaag aaaagccgat agactttttc tttctagtat gactcataaa atatacacag
    11221 aagagagaac tagctttgag caatttctgc aaaaactagt tctctcttct atttattatt
    11281 acgtattacg ctggttcact gactttcgca atcttccctt gttcgatata gaccattagg
    11341 atacttaggt ctgcagggtt tacgccactg attcggctag cttgggcaat tgtttctggt
    11401 tggattttct gcaatttttg ttttgcttct gttgctaatc cgttgattgc tgcgtagtcg
    11461 atgttttctg gaatcttttt cgcttccatg cgttttaatt tttctacttt ttccatggct
    11521 tttttgatgt agccttcata tttgatttgg atttctactt gttcgatttc ttttcgtgtc
    11581 aattcttcat tttctggaat aaaccgtaga agttctgcat aagtaatctc aggacgtctt
    11641 aaaaagtcgc ttgctagtac accatctttt aatggtgccg cattaatcgt tgctaaaaag
    11701 tcttggactt cttctgttgg tttgattcgt acattcatca aacgtttgat ttcttgttca
    11761 actgcttctt ttttcttcaa ataattcttg tattgatctt cttttactaa gcctacacgg
    11821 tgacccattt ctgttaatcg caaatctgca ttatcatgac gtaaaagcag acggtattca
    11881 gcacgtgaag tcagcaaacg ataaggttcg ttcgttcctt ttgttaccaa atcatcgatc
    11941 atgacaccga tgtaaccgtc acttcgtttg atcactaaag gttcttttcc ctgaactttc
    12001 aaagctgcat tgataccggc aatcagccct tgtccagccg cttcttcata accgcttgta
    12061 ccatttgttt gtccagctgt ataaagattt tcaacgactt tagtttctaa tgtcggacgg
    12121 agctggtgtg gctctactac gtcatattcg atagcatatc ctgtacgcat catttctact
    12181 ttttctaatc cagcaataga atgaagcatg ttgacttgaa catcctctgg taaagaagta
    12241 gataaacctt gaacatagac ttcttccgta tttaagcctt ctggttccaa aaataattga
    12301 tgacgtggtt tgtctgcaaa acgcacgatc ttatcttcga tggatggaca atagcgagcg
    12361 cctacccctt caacgatccc tgtaaacatt ggggcacgat gtaagttgtc tcgaataatt
    12421 tggtgagtcg tctcacctgt ataggtcagc cagcaagatt cctgatctag tttataggca
    12481 ctatctggtg tgctgaagct aaaatgattc ggctctttgt caccaggctg ctcttctgta
    12541 acagaatagt cgatcgtact tgattttact cgaggcggtg ttcctgtttt gaaccgttcg
    12601 atagcaaagc caagttcttt tagatgattc gctagtccaa tagaaggttg agagttattc
    12661 ggacctgatg aatatttcaa ttcaccgata atgatttctc cgcgaagtgc tgttccggct
    12721 gtaataacaa ctgctttggc ccggtagctc gctcctgtgg acgtcactac tccatgacaa
    12781 acaccatctt cgacgatcag ttcttcgacg attccttgtc ttagcgtcaa atttggttct
    12841 ttttcgatcg tatgtttcat ttctgttgaa taagcatgtt tgtctgcttg tgctcgcaat
    12901 gctcggacag ctgggccttt tcctgtgttc aacatacgca tttggatata ggttttatcg
    12961 atattcttac ccatttctcc gcctaacgca tcgatttctc gaacgacaac gcctttagct
    13021 ggtccgccta cagaaggatt acatggcatg aatgctacca tgtccaaatt tattgtcagt
    13081 aataatgttt ttgatcccat acgtgcagca gcaagtgcag cttctgaacc ggcgtgtcct
    13141 gcgccaacaa cgatcacgtc atacgcatct gcttcataat gttccaatcg tttgttcctc
    13201 cttttatttt cctaaacaaa attgactgaa taattgagtg attagctcat cttgtacact
    13261 gtcgccgaca acttctccta gatagtccca gcaacgcgtc atatctattt gaacgagatc
    13321 cacaggcatt cctgaatcaa tacctgcaat gacttcttca agtgagaggg aagctttttc
    13381 taacaacgcg atatggcggg tattagaaac ataagtagcg tctctttctc ctgtttctcc
    13441 gctgaagaat aaatcagaaa tagctgattc caaacgatct agtccgtcgt ttttagctac
    13501 ggaaattgaa aaaactggtt cattttcgat caatttcttc aatttttctt gttccagctg
    13561 tgctggcaaa tctgttttat ttagaagaat gatccgtttc aatccacttg tcgcttccaa
    13621 taattgttca tcttctgctg tcagtggttc gctttggttt agaacaagta agatcaagtc
    13681 tgcttctgct aatgcttttc ggcttctttc gaccccgatc cgttccacca catcttctgt
    13741 ttcccgaatt ccagctgtat cgattagctt gagcggtacc ccccgaacat tgacatactc
    13801 ttcgattaca tctcgagtcg ttcctgcaat atccgtaacg attgcttttt cttctcttaa
    13861 caaatggttt aacaaacttg atttgccgac atttggccgg ccgatgattg ccgtacttaa
    13921 cccttcccgc agaaccttcc cttgcttgga tgtagcaagt agcgcttgga ttctttgttg
    13981 gatcatctgc gctttttcaa gcaataactt cgtagtcaat tcttcgacat catcatattc
    14041 tggataatca atatttactt ctacttgtgc taatgtctct aaaatctctt gtctcaatga
    14101 gcggatcaaa gcagacagat tgccgtctaa ctgattaaga gctagtccca ttgctttgtc
    14161 tgtttttgcc cgaatcaaat ccatcacagc ttctgcctgt gaaaggtcaa cacgaccatt
    14221 caagaatgct cgttttgtga attcgcctgg ctcagctaat cttgcacctt ctcttaacaa
    14281 tagctgcaaa atttgattga ctacaacgat cccaccgtga caattgattt ctactacatc
    14341 ttcccgcgta aatgtctttg gtgctctcat cacggagacc atgacttcat cgacaagctg
    14401 attgctcttg ggatcaacga tatgtccata atggatcgta tgacttggta cttctgataa
    14461 tcgtttgttt ccagattgat aaactttatc tgctagttgc acagcttgat cgccacttaa
    14521 acgaacgata ctgattgcac cttctccagg tggggtagaa attgccgcaa tcgtatcaaa
    14581 ttctaaagta atttcagcca ttttttcact tcctttttca ttttgcgcac aaaaaaagtg
    14641 cccactccac cctttatgca caataaacgg tgaacttcgc actttgactg cttgttcttg
    14701 attaaattct ggtcatagat tatcacactt gctataagga atcaagtgtt taaaacaatt
    14761 agatgaagtt gagcctgttc tgcttccatt accttatatt taattgtcct tggatgtctc
    14821 tttgcgttct ttggtttatt cgaagagaag atactgtata aacaaggctt tatctttctt
    14881 aaaaaaaacg gtacactaaa tggtagcgct atctaaacta tacacttttg aaaggagaaa
    14941 atatgtgagc ccgttcagat ctgctgcatt ggaagaagaa agtgaccctc ggatcaacga
    15001 tgtcgtttta tctacaggga atatcaacca gaaatatatc gtccgcgata tcgtttttgt
    15061 tgtggaatgc ttagaagctg atttgtttga cccatctttt cccaaagatg accttttctt
    15121 aacaacgaag aggaaattaa agacgaaggt acttgaatat ggtgcgaatg cagtaatcaa
    15181 ctgtcatttt gagtatcagc aaaaaaaacg cgacggaaag tattttctgg aactttttgc
    15241 ttatggaaca gtgatccaat ttatccaaac aacgattggt taaatgaaac agacaaaaaa
    15301 agcggatcaa aactgaagac agtttcgatc cgcttttttc tatcaaagtt agtgactact
    15361 tcatcgtata ctctgataag aatgatttct agtaaaatat acttggtttg aaaaactttg
    15421 tcaacgtgat ttattcaaaa agtaatggaa tcaataatat tttttagcag gttctactac
    15481 taaataacga taaggttcct caccttccga atgtgttttt acatgttcat ctttgcttaa
    15541 tgcagcatgg atttgtttac gttcaaatgc aggcattggt tctaagaaaa caggctgccc
    15601 tgtccgtttt actttttcag ccgtgcgatc agctaaacgc tgaatgatct cttggcgctt
    15661 ttcacggtaa ttgcctacat tgactactac agacagctta tttcttgcta aacgatggat
    15721 gaagacttgt gccagatatt gcaatgcatt cagtgttttt ccatgtttcc caatcagtat
    15781 gccttgcttt tgtgtttcta aatggaaaac aaccatatta tttgattctc tttccatttt
    15841 gacaagagct ggagcatcaa gctgttttga aatttcagta agataaactg ccaattccgt
    15901 taaagctgct tcatcaccta gctcacttac ttcttctgtt ttttcttcaa gaatcgtttc
    15961 gttgactacc tcgatctcag ctgttggttc ttcttccaaa atttcttcta cagtctgttc
    16021 tactgtttca gaaacttttt cgctgatact tggctggatc gaaagccgtg cattctttcg
    16081 acccattcct aggaaacctt tttttccttc ttccagtatt tcgatttcga cttggtcttt
    16141 ggccaaaccc aaatcagcca gacctagttg gacagcttcg tctattgttg cagcttcata
    16201 aatcggcatt agcattacct cccctattat ttccgttttt tcttaggatt cattgctttc
    16261 ttaagagcac gttctctttc gcggattctc cgtgcttctt cttcacgctc ttttcgaatt
    16321 ttgaaagggt tgttgatcag cagcgtttgt acaacttgga aggcattgga aactacccag
    16381 tataatgaaa gaccgctggc taaattgaca cccatcacta aaatcattac aggcatcacg
    16441 aaattcatga tcttcattga tgcattcgat tcaatttgac tcatgctaga taaatacgta
    16501 cttgcaaaag taaagattgc tgctaaaatc ggcaaaataa atgttggatc cgcgttccct
    16561 aagttcaacc ataagaaatg cccttgtttc aactctggta cacgtgaaat agattgccat
    16621 aacgccatca agattggcat ttgtactaat aaaggaagac aaccagcata tggattgacg
    16681 ccattttctg catataaacg ctgctgctct tctcttaact tactttgtgt ttccggatct
    16741 cttgaactat attgttgctg cagtgctttt aatttcggtt gtaattcttg cgttttacgc
    16801 atgctctttg tttggaaatg catcaacggc aataggatca cgcggatgat cagtgtaaat
    16861 aaaatgatcc ctatccctgc attaccaaat gacaacgcct tgatcgcctc agcaaagtaa
    16921 taaacaatat aacgatccca aattcctgta ctctgtgcgc tgacttcccc agttccgcaa
    16981 ccggataaga cgatgaccaa actaacgata ccggccatca aaagtaaacg tttgtatttc
    17041 ttcacttact cttttcctct ctgtcgatta tttttgcaag ttttaagacg tggactaaat
    17101 tggagcgaac ctcatcataa gtcaacccct tcacacttgg tctagcaatc acaataaaat
    17161 ccaactccgg atcaatttct tcttttatag aataaactgc tgctcggatt tttcgtttga
    17221 cttcattgcg gtttaccgcg ttgccgacct tttttcctac agataaacca actcgaaaat
    17281 gaggctgtcc cggtttttcc aaacggtaaa cgacgaattt gcggttagca aaagaatctc
    17341 ccgtatggaa cacggcttga aactctcgtt ctttttttac tcgatacgct tttctcatta
    17401 gttttctttc cttttctatc tctcacttac gctcataatc ataccataat tacgattgtc
    17461 ctttcatcat ttccctaaaa aagagtataa aaaaaaccac tgaaacccca gtggcctaag
    17521 ctgaaagaac ttttcttcct ttgcgacgac ggctagctaa cacacgacgt ccatttttag
    17581 tactcatacg tttacggaat ccgtgaactt tttgacgttt acgtttattt ggttggtatg
    17641 ttcttttcat atattccacc tccaaaatgt tcttatctat tttataaaca cagacatact
    17701 ttgatagtat aacgacaaag tagtgatttt gtcaatataa aatagaagat cgattgttaa
    17761 aacttatcca caagattatt tttttctatg ggaatcctat gtggaaaaag ttatgcacag
    17821 ggattatcca cagataataa aaggctggtt ttacacagaa aacacacgtg taaaaaaagt
    17881 aatccacaat caggcgattt cctgtgtatt ccgtctccta actattggta tatctgtatt
    17941 ttttatccac agacgcaaga ggaaatctat tggtttttac gagttttcca cctgtgcatt
    18001 agtggtgtgg aaaacttttt ttgacaactg tgtttttctc ttttttcttt ttgtcgattg
    18061 tgtggataat tgtaattaaa aaattttttt cttcggtttc tgtggaaaac tattgaaaaa
    18121 aatgctaaat tagtactagt tattcacttt ttaatgaagg aggaggcctt atatggtatc
    18181 cctcgatgct ttatggaatg aattaaaagc aacataccaa aaggatttat cgcctgctag
    18241 ctataataca tggatcgaaa cagcgaaccc tcgaaccctt gatcaaaacc agctcgttgt
    18301 tgaggttcct agcaagattc ataaagaata ttgggaaaaa aacctggcga caaaaatcgt
    18361 tgaagtcggt tatatgttat caggaaatga gattatccct cgctttatta ctggcgaaga
    18421 agcagagcaa gaagaagtta tagaagaaaa aaatccaaaa gttgtggcac ctagtccgct
    18481 aaaaaaagcc atgctgaacc ctaaatatac ctttgatacg tttgtcattg gtaaaggaaa
    18541 ccagatggct catgcagcag cgctcgttgt agcagaagat ccaggttcta tttataaccc
    18601 gctcttcttt tatggaggcg tgggacttgg taagactcac ttgatgcatg caattggtca
    18661 tcaaatgctg caaagtcagc ctaatgccaa agtaaaatat gtaagcagtg aaacttttgc
    18721 taatgatttc atcaattcga tccaaaacaa aacagcagaa gagttccgtc aagaatatcg
    18781 aaatgttgat ttactgctag tggatgacat tcaatttttt gcagaaaaag aagctacgca
    18841 agaagagttt ttccatactt tcaatgcctt gtacaatgaa gggaaacaaa tcgtactaac
    18901 cagtgatcgg ttgccaaacg agattccgaa actgcaagaa cgccttgttt ctcgctttgc
    18961 atgggggctg tctgttgata ttacaccgcc ggatcttgaa acaagaacag cgattttacg
    19021 taaaaaagcg gcggctgaac gtctagagat cccagatgac acactaagct acatcgcagg
    19081 acaaattgat tctaacattc gtgaattaga aggtgcgctt gttcgagtgc aagcttttgc
    19141 tgcgatgaat agtgaagata tttcgactag tctagctgca gacgcactga aaacgttaaa
    19201 atcaggaaaa ggacatcctc aattgtcaat cctgcagatc caagaagaag tggcaaagta
    19261 ttatcacatc caattgaaag atctcaaagg aaagaaacgc gtcaaatcta ttgttgttcc
    19321 tagacaaatc gctatgtatt tggcacgtga gctcacagat aattctttac cgaaaatcgg
    19381 agctgaattc ggtggcaagg atcatacaac ggttatccat gctcatgaaa aaatccagca
    19441 gctgatggat tctagtgtca gtatgcaaaa tgaagtatca gaaatcaaaa actttttact
    19501 aaattaattt gtcgattcta tcaactgaaa atagcatgtg gataaaagaa aaaaacgttc
    19561 aaaagttata cacacttttt ccacagctgt tttttcgttt ctctcttacg ttttttgagt
    19621 tttccacaga atcaacatgc cctattactt ttattattct ttttaactaa taataaataa
    19681 acaaacattc aaaggagata catcatgaaa gttactttaa accgagctag ctttatgcag
    19741 gaattgcaaa ctgttcaacg agctatttca agcaaaacca cgatccctat tttgacaggt
    19801 gtaaaaatca cactgacaca agaaggtttg actttgacgg ggagcaacgc tgatatatca
    19861 attgaaactt ttttgtctgt tgaaaacgaa aaagcaaata tgcaaatcga atctactggt
    19921 tccattgttt tacaagcacg tttctttagc gaaatcattc ggagacttcc tgaagaaaca
    19981 tttactttag aagttttaga aaataaacaa gtagcgatca cttctggaaa agcgaatttt
    20041 atcgtaaatg gattagatgc agataactat cctcatcttc ctgttgtcga aagccataac
    20101 cagatgaaat tacctgtaca cgtattgact aaactaatca acgaaacagt ttttgctgtc
    20161 tcacaacatg agagtcgtcc aatcttgaca ggtgtgcatt tcattttatc tgataattct
    20221 ttattagctg tagctactga ttctcaccgt ctaagtcaac gcgtgattcc agtagaacaa
    20281 gcggctgatc attttgatat tgttattcct ggaaaaagtt tgatcgaatt atctcgctca
    20341 ttaacaaatg aagaagaaat cgtcgaaatc agcattatgg aaaaccaagt gttgttcaaa
    20401 acagaaacga tgtatttcta ttcccgtttg ctagaaggaa actatccaga taccaatcgt
    20461 ttgattcctt caagttttaa tacagaggtt gaattttctg ttcctagctt tttagcggcg
    20521 atcgaacggg cttctttact ttctcatgaa ggccgtaaca acatcgttcg tttatctatt
    20581 cgtccagatg cggttgtttt atacggaaat tcgccagaaa tcgggaaagt agaagaaagc
    20641 ttgagttata cagcaagcag tggcgatcca ttagatattt ctttcaatcc ggattatatg
    20701 aaagcagcgt tgcgtgcatt tggagatatg agcatcaaag tgaaatttat ctctgctatt
    20761 cgtccattta cattggaacc aacggaagat ggcgttcaat ttattcagct gattactcct
    20821 gtacgtacaa attaacaatg attttcaatg ttcttgaaaa tttaattgaa aagaaataga
    20881 aaaaataaaa ttttaaacag aaggccctaa aatctctttt aaggctttct gtttattttt
    20941 gtttaaatca ccttttttag attaaatcgt cttatatgga aaaatatatg gataaatttg
    21001 ttttattgcg aaaaaaaagg tataatagag tatactttta ataatgaaaa tgagggatgg
    21061 gcattgaaac aacaagtcat tctaaatacg gaattcatga cgttgggcca aatgttgaaa
    21121 gaagtcacag ttatcggtag tggcggacaa gcaaaatggt atttggcaga aaacactgtg
    21181 ttagtcgatg gcgaaccaga aaatcgtcgt ggacgcaaac tttatcccgg catgatggtt
    21241 gaagttccag aagtaggaac tttttttatg gtaaaaaaag gaaacgacga taatgaggct
    21301 gaataagctg tatttaaaaa attatcgaaa ttacgaagag ctgaacatcg aattttccaa
    21361 gaacctcgtc atctttttag gagaaaatgc gcaaggaaaa acgaatcttt tggaaagtat
    21421 ctacgtatta gctatgacgc gaagtcatcg gacaagtagt gagcaggaat tgattggctg
    21481 gactgacgat caggcgatga ttcaaggaga gatcacaaaa ggttcatcca ctcttccatt
    21541 agaaatctta ttatctaaaa aaggccgaaa aaccaagatc aaccatatcg aacagaaaaa
    21601 gttaagtact tacgtgggac aaatgaatgt cattttattt gcaccagaag atctttcttt
    21661 agtaaaaggc agcccgcaga tcaggcggaa gtttttagat atggaattag gccagatcaa
    21721 tccaatctat ctttatgatc ttgtccaata ccaagcaatt ttgaaacaaa gaaaccagta
    21781 cttgaagcaa ttgaatgaaa aaaaacaaac tgatttgctt tatttggata ttttgtccga
    21841 acaactagca gcgtttggca gcaaagtatt gaaagcaaga gcacagttcg tcaagcgatt
    21901 ggagttttgg gctaattctt tgcatcagca aatcacccat caaaaagagc agctggaaat
    21961 cgagtatctc accgcagtcg attctttaga aacacatacg caagagcaaa tccaagaaca
    22021 atttctcgca cttttgaacc aaaataaaaa gaaagacctt tttcggggaa caacgacagt
    22081 tggtcctcat cgtgacgatc tttccttttt tattaatcag aaaaatgtgc agacttatgg
    22141 atcgcaagga cagcaaagaa caactgcact aagtgtcaaa ttagcagaaa tcgatttgat
    22201 taaagaagag acaggggaat atccaatcct tttgttagat gatgtgatga gcgagctgga
    22261 tgataatcgt caactccatc tgctagaaac aatcgaagga aaagttcaaa cgttcttaac
    22321 aacaacgaca cttgaccatg taaaaaataa aatgactgtt gaacctgaga ttttttatgt
    22381 tcaacaaggg aaactaaaag gagaagaaac tgaatgacag aagaaagaag tttagtagaa
    22441 cgcgctaaag agtatgatgc cagtcagatt caagtacttg aaggacttga agccgttcgt
    22501 aaacgacctg gcatgtatat cggttcgact agttctgagg gtctgcatca ccttgtttgg
    22561 gaaattgtcg ataattcgat cgatgaagta ttagcaggtt ttgcaacgaa aatccatgtg
    22621 attattgaaa aagacaacag tatcaccgtt attgacgacg gtcgggggat acctgtagat
    22681 atccaggcaa aaactggacg tcctgcagtt gaaacggtct ttactgtttt acatgctgga
    22741 ggaaaattcg gcggtggtgg gtacaaagtt tctggtggac ttcacggagt aggatcttct
    22801 gttgtaaacg cgctttctac acagcttgat gtcaaagtat acaaagaagg aaatgtgtac
    22861 tatcaagaat atcgtcgtgg agctgtagtg gacgacttaa agattatcga acaaacggat
    22921 cgccatggaa caacggttca ctttacgcca gaccctgaaa tttttacaga gacaactgag
    22981 tttgattttt caaaattggc tacccggatc cgtgaacttg ctttcttgaa ccgcgggatg
    23041 cgtatatcga ttgaagacaa acgagaagaa gaaccagtca tcaatgaata ccattatgac
    23101 ggcgggatca aaagttatgt cgaatacttg aatgcaaaca aaaccgtgat ttttccagaa
    23161 cctgtatatt tggaaggtga acagcaagat atcgcagttg aagtctctat gcaatataca
    23221 gacggctacc attccaatat catgagtttt gccaacaata tccatacgta tgaaggtgga
    23281 acacatgaat caggattcaa gactgcttta actcgtgtga taaatgatta tgcccgaaaa
    23341 caaaagctga tgaaagaaaa tgacgacaac ttgaccggtg aagacgtgcg agaaggattg
    23401 acagctgtta tttctatcaa gcatcctgac cctcaattcg aaggacaaac aaaaacaaaa
    23461 ttgggcaatt ccgaagtacg gacagtcact gatcggttat ttagcgaaca tttcatgaaa
    23521 ttcttgctgg aaaacccaag tgtgggccgg caaattgttg aaaaaggatt actagcttcc
    23581 agagctcgtc tagcagcgaa acgtgcgcgt gaagtaacga gacgtaaagg tgctttagag
    23641 attagtaatc tgccaggaaa attagcggac tgctctagca acgatcctga aaaatgcgaa
    23701 ttgttcatcg tcgaaggaga ttcggccggc ggatcagcta agcaaggtcg taatcgagag
    23761 tttcaagcca tcttgcctat tcgtgggaaa atcttgaatg ttgaaaaagc aagcatggac
    23821 aagatcttgg caaatgaaga gatccggtct ttatttactg ctatgggtac agggtttgga
    23881 gaagattttg atgtatcgaa agctcgctac cacaaattag ttatcatgac tgatgccgat
    23941 gtcgacggcg cgcatatccg tacattgctg ctgactcttt tttatcgtta catgcgtcct
    24001 attgttgaag ctggatacgt ctatattgct cagccacctt tatacggggt aaaacaaggg
    24061 aagaatatta cgtatgtaca accaggaaaa aatgcagaag aagagttgaa acaagtcgtt
    24121 gcttcacttc ctgcctcacc aaaaccaagc gttcagcgtt ataaaggact tggagaaatg
    24181 gatgatcatc aactatggga aacaactatg gacccaagca atcggatgat ggcccgcgtc
    24241 agtgtggacg atgctattgc agcagatcag atatttgaaa tgttgatggg ggatcgagta
    24301 gaaccccgtc gtgcattcat cgaggaaaat gcacactatg tgaagaactt agatatttaa
    24361 tcatgaagga ggaaaaagaa tgagtgaaga aatcagagaa aacatccatg atgtcaatct
    24421 aaccagcgaa atgaaagact ccttcatcga ttatgcgatg agtgttattg tcgcacgtgc
    24481 acttcctgat gtcagagatg gtctgaaacc cgttcatcgc cgtattttat acggcatgaa
    24541 cgaattaggt gtaacaccgg ataaagccca taaaaaatct gccagaatcg tcggagatgt
    24601 catgggtaaa taccatcctc atggagacat tgcgatctat gagtcaatgg tacggatggc
    24661 acagccattc agctaccgtt atatgctagt ggatggtcat ggaaactttg gttctgttga
    24721 cggtgacggt gccgccgcta tgcgttatac cgaagcacgg atgagcaaaa tcgctacaga
    24781 aatgctgcgt gatatcaata agaacactgt tgatttccaa agcaactatg atgatacaga
    24841 aaaagaacca gttgtactac ctgctcgctt cccgaatctt ttagtgaacg gaacaacggg
    24901 gatcgccgta ggtatggcca cgaatatccc acctcataac ttgtctgagg tagtagcggc
    24961 tatcgatttg ctgatggata atcctgatgt gacgaccaat gaattgatgg aagtattacc
    25021 tggaccagat tttccaacag gtggtttggt tatggggaaa tcaggaatcc gaagagcgta
    25081 tgaaacaggt aagggttcta ttaccgtacg tgcaaaagtt gagattactg agatgccaaa
    25141 tggtaaagaa cggatcatcg tgacagaatt gccttatatg gtcaataaag caaaattgat
    25201 cgaacggatt tctgaactgc acagagacaa gcgtatcgaa gggatcacag atttgcggga
    25261 cgaatcttcc cgtgaaggta tgcggatcgt cattgacatc cgtcgtgacg ccagcgcatc
    25321 cgttatcttg aataacttat ataaattgac atccttgcaa aattcatttg gattcaatat
    25381 gttggcgatc gaaaaaggtg tgccgaaagt tttgagcttg aaacaaatcc ttgaaaacta
    25441 tatcgaacat caacgcgaag tcatcacacg acggacggtc tttgaaaaag aaaaagcaga
    25501 agcaagagca cacatcttag aaggtttgcg tattgctttg gatcatatcg atgaaatcat
    25561 tgcgattatc cgcgggtcta aatcagatga cgaggcaaaa gcaacaatga tcgaacggtt
    25621 cgatttatcc gatcgccagt ctcaagctat tttagatatg cgtttacgcc gattgaccgg
    25681 tttggaacgg gaaaaaatcg aaaatgaata ccaagaactc ttaaaactga ttgaagattt
    25741 aactgacatt ctggcaagac cagaacgtgt tacagaaatc atcaagaatg aattagcaga
    25801 cttgaatcaa cgatttggtg ataaacgacg cacagaatta ctagtcggtg aggtcttaag
    25861 tctggaagat gaagatttaa tcgaagaaga agaaatcgtt attacattga caaacaatgg
    25921 ttacatcaaa cgtttggcaa ataatgaatt cagagctcaa cgaaggggtg gacgtggtgt
    25981 ccaaggaatg ggtatccaca atgacgactt cgtcaaaaat ctggtttctt gttctaccca
    26041 tgatacattg ttattcttta cgaataacgg aaaagtgtat cgggcaaaag gatacgaaat
    26101 tccagaatac gggcgtacag ccaaaggaat cccaatcatc aatttactag gtatcgattc
    26161 gagtgaaagc atccaggcaa tcattgcggt gacgggagaa gcagaagaag gccactatct
    26221 cttctttacg acgcgtcaag gtacagtaaa acgtacttct gtcactgctt ttgctaacat
    26281 ccgaagtaat ggtctgatcg caattggttt gaaagaagac gatgagctcg tcaatgtgct
    26341 actgacagat ggtcaatcta atatcattat tggaacacat aacggctact ctgtcacttt
    26401 caaagaagaa gcagtccgcg atatgggacg tacagcttcc ggggtcagag ggatacgcct
    26461 gcgtgaggaa gattatgtcg ttggggcagc cttaatgaag gaaaatcaag aagtattagt
    26521 gatcacagaa aaaggttacg gaaaacggac aaaagtatcg gaatatccag tgaaaggccg
    26581 cggaggtaaa gggatcaaaa cggcgaacat cactgaaaaa aatggtccac tagctggatt
    26641 gaccactgtt tctggagatg aagacatcat gctgattaca gataaaggtg tcattatccg
    26701 tttcaacgta tcgactgttt ctcaaactgg acgttcaaca ttaggcgtac gtttgatgaa
    26761 gatggaagct gatacgaaag ttgtcacgat ggcagcagtc gaaccagaag ctgcagaaca
    26821 aacagttgaa gaacaaggat cagaagaata accatcaaga ctaagtgtgt aagctagcaa
    26881 gaataaccga ttaaaaaatc tggttattcc tgctggcttt tttgattagt tgaatttctc
    26941 ttaaaatatt cgaaactgtt cattctgtat tgcttttgac caaaatagag tgtataatta
    27001 gagcttgtga gtagtctaat taattagata actctccttg ctcttaggaa cctaagagcc
    27061 gaaagtccac agggaggtga atatcaatgg aaaacacgaa atatgaaatc atgtacatta
    27121 ttcgtccaaa cattgatgaa gaagcaaaaa ctgctttaat cgaacgtttc gatgcaatct
    27181 tgaaagataa cggagcagaa gttatcgaat caaaagattg ggaaaaacgc cgtttcgctt
    27241 atgaaatgaa cggttaccgt gaaggtatct atcacatcgt taaagtatct tctccatcat
    27301 cagcaaatgc tgtcaatgaa tttgaccgtc ttgctaaaat caacgacgat atcattcgtc
    27361 acatgattgt taaagaagaa gcttaattgt tgtttcatgt gaaacaatag aaaacgatga
    27421 gaggagatga ctattttgat taataatgtt gtattagttg gacgtctgac gaaagatcca
    27481 gatttgcgtt acacagcaag tggtacggca gtcgcaacat ttactttggc tgtgaatcgt
    27541 aattttacaa accaaagcgg caatcgcgag gctgatttta tcaactgtgt gatttggcgc
    27601 aaatcagcag aaactttagc aaactatgct cgtaaaggaa cacttttagg tgttactgga
    27661 cgtatccaaa ctcgttctta tgataatcag caaggacaac gtgtatacgt gacagaagta
    27721 gtagctgaca acttccaatt gctggaaagt cgttctgcat ctgaaaatcg ccaacaaagc
    27781 ggcggattcc aaagttctgg tcaatcagct ggcggatttg gcggaaacaa taactctaat
    27841 caaacatctc aatcatccaa cggtatgcct gatttcgacc gtgatacttc tgacccattc
    27901 ggctcaagtt caacgatcga catttcagac gatgatttac cattctaaac gaatttgata
    27961 ggagggaaat agaatggcac aacaaagaag aggcggacgt aaacgtcgta aagtagacta
    28021 cattgctgct aaccatattg aatatatcga ttataaagat atcgaattac taaaaagatt
    28081 tatttcagaa cgcggtaaaa tcttgccacg tcgtgtaact ggtacaggcg ctaaaaacca
    28141 acgtaaaatt actgttgcaa tcaaacgcgc tcgtatcatg ggcttgctac ctttcgttag
    28201 cgaagactaa taaatcctag gaattgaatg actgatcctt ttgatcagct attcaattcc
    28261 tttttttctg taaaaataac ctctattcgt ttagtcgctc gaatgacgaa agttatggta
    28321 aaatcaaatt agtcatatat gggaggaaaa ttatggaaaa taaaaacaat catttatccc
    28381 cgaaacctct cttccttatc ctgtttatat taatcgaagt attggtagtc ttaattttac
    28441 catttcgttt tgttagcgct tcggtcattc tattgatgaa tttatggggt gttcgctatt
    28501 ggttgaaaat gaaacgttgg cttattttaa ataaacgtga aaaaatccag caggcttcga
    28561 ctgtagctga aaccaatcta gcttatgtct cagaagtcat gcctgtagga gtgattgctt
    28621 atcagccatc aagccaaaaa atcgaatggc tcaatccatt agcattgaaa atcaaagatc
    28681 aaagtggatt aacagaggaa gaattgctag atactttttt tactgcaaaa gaaaaaaaga
    28741 aaaaccaagt ctatctagca gataaaacat ttcagtttga tatggataca gtaaagggtg
    28801 ttgtttattt taatgatatt acccaaaacc agttgttaaa actccatcag acgaacagtc
    28861 agccggtgat tggtatttta tctattgata actatgcaga tgccattgat aaattagatg
    28921 acaaagagat ttcttatttg aactctttcg tcactacact agtttctgac tggatgaatg
    28981 aataccatgt ctttttcaaa agaatcaacg cagaacggtt tttcttcgta gcacagatga
    29041 tcgatctgaa aaagatgatg gataaaaaat ttgatttgct ggatcgcttt cgagcagaag
    29101 ctgaaaaaca gagctcgcct atcaccctta gtatggggat tgcttatggc tcaagaaccc
    29161 tcgatgaaat cggtggagaa gctcagaata atttagatgt tgctttagtc cgtggaggcg
    29221 accaagttgt cgtgaaagag gtaaaagaag gagcaaaacc tgtttattat ggggggaaat
    29281 ctgctaagaa tgtcaaacgc acgcgtgttc gttctcgagc gatgagtacc gctttgcgtc
    29341 ggatttttga tgaaacaggc gatgtgttta tcatggggca caagtttccg gatatggatg
    29401 ctttaggctc tgcttttggt gtcagttgtc tggcacgctt caaccagaaa aaagcttaca
    29461 tcgttatcaa tgagaacgag atcattccag atgtagaacg gtgtcttgaa gaaatccata
    29521 agcatcctga attagaaatg caactaatct ctccagaacg tgccatagag ctgaaaaaag
    29581 aagaggacct cctcgtgatg gtggattacc ataaaccttc tctatcgatc tctcagggcc
    29641 tatatgagca atttgataag attgtgatca ttgatcacca tcgtcgtggg gaagagtttc
    29701 caagtaaacc tctgctgact tatattgaat cttcagcttc ttcagcttca gagctagttt
    29761 ctgagctgat ccaataccaa agcagcaaaa caaatcgctt agatcgtttt gaggccacat
    29821 tattgcttgc agggatggtt gtagatacaa atagtttcaa tgtccgtaca actgcacgaa
    29881 cttttgacgt tgccagctat ttaaggtctt gtggagcaga cgcatcctta gtacaatatt
    29941 tattgagctc ggatttagcg acatacttgg agatcagcaa tttgattgct ggaagcgact
    30001 atgtgacttc tgatattgtt gtggcggcag ggacagacga aaaagagtat aatagtgtta
    30061 tagcagcaaa aacagcagat acattgcttt cgatgatcaa tatcaatgca gctttcgtca
    30121 ttacaaagcg gacagacggc ttgattggga tcagtgccag aagtaccggc acgatcaacg
    30181 tccaaatcat catggaatcc ttaggtggcg gaggacactt cacaaacgca gctactcaac
    30241 tggaaaatac gacagttgag gaaacaaaac agaagctatt tgctgcaatc aaagaaaata
    30301 tgaatcaaat atatgataag gagtaatcca aatgaaagta atttttttac aagatgtaaa
    30361 aggaaaaggt aaaaaagggg acatcaaaga agttccagca ggttatgcac aaaattatct
    30421 aatcaaaaat aacttggcaa aagaagcaac gaaaagcagt atcagtcagt tagcaggaca
    30481 aaagaaagca caagaaaaac acgaagctga aatcttggaa gaagctaaaa agttaaaaga
    30541 attcttggaa aaagaagata ctgtagtcga attgaaggcg aaagcagggg aagacagccg
    30601 tttgtttggt tcgatccctt caaaacaaat tgctgaaggg ttaaacaaac aatacaaaat
    30661 caaattagat aaacgaaaaa tcgaattaga aaatccgatc cgttcactag gctacacaaa
    30721 cgtacctgtt aagcttcatc atgaggtgac ggctacgatc aaagttcatg tcgtacaaga
    30781 ataacaaatc ttgatagcga tgttcagctg gtaagtataa ccagaaggaa aaaagacaaa
    30841 ccgaaattta tctgagaaaa cagctgcagc aatgaaagca aaagctgcaa atcgatttca
    30901 gattgatttc ggtttctttt ttgggaacag caaaatagca taaagaaaac gagtataata
    30961 aaagagaact atttttttat tacactttcg gtacgaatga tgacttttct ctcattttct
    31021 tgtaaaatga agcaattgaa gacaaggagc gatacgtatg aatgaagtgt ggcaggatcg
    31081 aatcccaccg caaaatattg aagctgaaca agcggtttta ggctctgttt ttcttgacgc
    31141 agaaacgatc attgatgcca tggagtacat agagccaaaa gacttttatc gtcgtggcca
    31201 tcaaattatt tttgagacaa tgatcgagtt aaatgatcgt aatgaagcga ttgacgtggt
    31261 tactgtcaaa gaccggctgg aacaagccaa tttgttagaa gatgccggtg gtttaagtta
    31321 tctctcagac ttggcattag cagttccgac agcagcaaat atcgtctact atgcgaaaat
    31381 cgttgaagaa aaatcattgc tgcgtacatt gatccagaca gcaacaggaa tcgttacgaa
    31441 aggattcgag caaggagaag aagttcaaac aattttagat gatgccgaac gcagtatctt
    31501 ggaggtttcg gaaaaaagaa atcgaagcgg ctttttatct attgctgaag tactgaactc
    31561 cacgattgaa aatatcgatc gactagctca aaacaatgaa gaaatcacag gtctaccaac
    31621 tggctatcaa ggtctggata aaatgacagc aggtttccag cccgaagaac taatcattct
    31681 tgctgcacgt ccagcggtag gtaaaacagc gtttgcattg aatattgctc aaaatgtagg
    31741 aacaaaaaca gatcgttctg ttgcgatatt tagtttggaa atgggggcag aatctcttgt
    31801 aaatcggatg ctttgtgcag aaggttcgat tgaggccagc catttacgta caggtcagct
    31861 ttcggaagaa gaatggtcga atctgatcgt tgcgatgggg agtctttctc gagcaaatat
    31921 ttttatcgat gatacaccag ggatcaaaat ctctgaaatc cgtgcgaaat gtcggaaact
    31981 agcgcaagaa aaaggcaatc taggcttgat attgatcgat tatttgcagt tgattgaagg
    32041 taacggtaaa gagaaccgtc aacaagaagt atcagatatc tctcgccaac tgaaaaaact
    32101 ggcgaaagag ctgaaagtac ctgtcattgc tctttcacag ttatctcgtg gagtagaaca
    32161 gcgacaagac aaacgaccag tgctaagtga tattcgtgaa tccggatcaa ttgaacaaga
    32221 tgcggacatc gtagccttct tgtatcgaga tgattattat gaaagagaag gcggagatga
    32281 cgatgatgga aacgagccaa aaaatgacaa cgtcatcgaa gtcatcatcg agaagaaccg
    32341 aagcggtgca cgaggaacag ttgaactact gtttatcaaa gaatacaata agttttcgtc
    32401 gctttcgcca agagaagaat tttaaagaag aggttgtgag ccttgagcaa tcccgagagc
    32461 tctcgtaaga agatgcaagg agtaaccgca ggaacgacta gagtacttaa tttaaacaga
    32521 tctccaaagg gattgtgaca aaagtcttgt cacagtccct ttttccgaat aaacggtgtt
    32581 caatcagctg acccttggta ttagctcagc aaacgtcaaa atatgaggaa ctattttgac
    32641 gtttgtttct ctaatactca ggtcaaaacg ctgatcttac gacctctttt ccttaaacgg
    32701 tgttctcctg ccaactcctc ggtcacaagt cacaatatca tcaaacatga gaagcagttt
    32761 tttgaaattg ctccttgtga ctcagagtta aacggcaggc tcacaacctc tttcttaaac
    32821 ggtgttcaaa agcttgcatc tgcggtaata cgcccaatca aacgaaaaat atgagaagct
    32881 atttttgttt ggctgttctt attacttgat gcaggacagc tttttcacaa cctctgattc
    32941 acggtgttcc aaaagtagct tcttcgaaaa ataagtcgaa atttcacaaa aatttgagaa
    33001 gcaattttcg tacattcctt cttatttctt gaagctgacc acttctgtta caacctcttt
    33061 tttaataacc attctaaaaa gataaagttc gttttttaag cgtttttata aaataaaaat
    33121 aaaaatcatt cgattttatt tgaaatatgt atctattatt gatacaatgg ttcaggatta
    33181 aataatatag aacgaggtgt tcacatgtca tctgtagtag tagtaggtac gcaatggggc
    33241 gatgaaggga aaggaaaaat cacagacttt ctcagtgaaa atgctgaagt gattgcacgg
    33301 taccaaggtg gcgacaatgc agggcacaca attaagttta acggtgtgac ttataaactg
    33361 catttgattc cttctgggat tttttataaa gacaagatca gtgtgatcgg aaacggcgtt
    33421 gtagtcaatc ctaaatcatt ggttaaagaa ttagcttatt tacatgaaaa aggtgtagaa
    33481 acaagtaatt tgcgtatttc tgaccgcgcg cacgtaatct tgccttatca tatcaaattg
    33541 gaccaattac aagaagatgc taagggcgaa aataagattg gtacaacgat taaaggaatc
    33601 ggacctgcct atatggataa agcagctcgt gtaggtatcc gtattgcaga cttattagat
    33661 aaagaaattt ttgaagaacg tcttcgtttg aatctagatg acaaaaaccg tcaatttgta
    33721 aaaatgtttg attcagaacc acttgatttt gacgatattt ttgaagaata ttatgaatac
    33781 ggacaacaaa tcaaacaata cgtcacagat acttccgtta tcttgaatga tgctttggat
    33841 gcaggcaaac gcgtgttgtt tgaaggcgca caaggtgtaa tgctggatat cgaccaggga
    33901 acttatcctt ttgttacttc ttctaaccca gtagccggtg gggtaacaat cggaagcggt
    33961 gtagggcctt caaaaatcaa taaagttgtc ggcgtctgca aagcctatac gtcacgtgtc
    34021 ggtgatggac catttccaac tgaattattt gatgaaacag gagaacaaat ccgcgaagta
    34081 ggacgagaat acggtacgac aactggtcgt ccgcgtcgtg taggctggtt tgattctgta
    34141 gttatgcgcc attctaaacg agtttcaggt attaccaacc tatctttaaa ctcgatcgat
    34201 gtattaagtg gattggaaac agtaaaaatt tgtacggctt atgaattaga tggcgaacta
    34261 atctaccact atccagcaag cttgaaagag ttgaaccgct gcaaaccaat ctatgaagaa
    34321 ttgccaggct ggtcagaaga cattacagga tgcaaaacac ttgcagaact gccagaaaac
    34381 gcacgtaact atgtacgtcg tatctcagag cttgttggtg tacgtatctc aacattttct
    34441 gtcggacctg atagaacaca aacaaacatc ttagaaagcg tctgggcgca aatctaagga
    34501 taaaaacgac gactgaatca ggacgttctt accagagcga gtcgaagtgt ttatctatac
    34561 gataataata acttgaagag gtagtgaaag agacgtttag ccttattgcc gataggctgt
    34621 ctcttgaaca ctgtttattc agaataaggg actgtgacaa aaaccgtgtc acagtccctt
    34681 tttccgaaca aacggtgttc aaaagcttgc atctacggca ctaagcccaa tcagatgaaa
    34741 aatatggagt gctattttta tctgattgtt cttactgctt gatgcagagc agctttttca
    34801 caacctctcc actaaacggt gttctcctgc caactcctcg gtcacaagtc acaatattca
    34861 acaaacatga gaagctgttt tttgaaattg ttccttgtga ctcggagcta aacggcaggc
    34921 tcacaacctc ttggtaaacg gtgttcaatc agctgaccct cggtattagc tcaaaaaacg
    34981 gcaaaatatg aggaactatt ttttccgttt ttttctctaa tactcaggtc aaaacgctga
    35041 tttcacaacc tctgattcac ggtgttccag aagtagcttc tacggaaata agccgaaatt
    35101 tcacaaaaat ttgagaagca attttcgtaa attccttctt atttcttgaa gctgaccact
    35161 tctgtcacaa cctcttattt tgattagaac ttgcgaaaat aaaccggtta aagtgtgtct
    35221 agcgcaagag atttgctata atagacgata agggtaaaat gtaacgagaa ggggttgaaa
    35281 aatgtgtatc aaattgtaac agattcttgt tgtgatctgc cataccatgt attagcaaaa
    35341 gaaaaagttg attttttatc aatgaatctc caattgaatg atcaagagtt agtagatgac
    35401 ctcggaaaga cattcaatta tgaccgctac atgaatcagc tgaaaaatgg tgcgatgcct
    35461 acgacatcac aaatcaatgt tggccgatat ttagaatttt tccgttctta tgcggaaaaa
    35521 cagattcctg ttttatatgt ggctttttcc agtggattaa gcggctctta cagtagtgcg
    35581 cttcaagcgg ttgaattgct gaaggaagaa tatgaagacc ctgagatcta tgtttttgat
    35641 acaaaagctg caagtattgg ggaagggctt ttagtattgg aagctgtaag attgaaaaaa
    35701 gaaggtaaaa gcatcgaaga aaccctagag tggctggaaa gtaattattt gaaagtccat
    35761 tcatgggtaa cagtagacga tctgaaatat ctagaacgag gcggtaggat ttccaaagca
    35821 agtgctactc ttggcagtct attgagtgta aagcctatta ttacggtgga taaagaagga
    35881 aggctgacga atgttgacaa agttcgagga agaaacaagt cgatccagac aatcgtcaat
    35941 gagacggtaa aaacaatcgt tgcaccactt gatcaaatcg tttatgtggc ttacgctggg
    36001 gatctagaga gcgcggagaa ggcaaaacgg cttttggaag agcagataaa gatgaaagat
    36061 gtgaagcttt atcccttagg accaacgatc gccagtcaca caggctatgg ctgtatcgcg
    36121 attttttcga tgggaatcag tcgataaaag cagtaactca ttctgtaaaa aagcccagaa
    36181 attgattgta acaaaaacag cgactcttaa aagatagatg agcatcctat ctcttagagg
    36241 tcgctgtttt tttatttttt aagctaaata ggccattatg cccataactc gttcaattgt
    36301 ttttgaatat ctgcattttc caaaaattca tcatatgtcg tatcagctcg atcgatcaca
    36361 cctttatccg aaacggcaat gatgcggttt gccaatgttt ggataaattg atggtcatgt
    36421 gacgcaaata agattgagcc agtaaatgcc atcaagccgt cattcaatgc tgtgattgat
    36481 tctaagtcta aatggttcgt tggatcatct aatacaagta cgttagcttt cgaaagcatc
    36541 atttttgaaa gcatgcaacg gactttctct ccaccagata aaacattcac tggtttcaat
    36601 acttcttcac cagaaaacaa cattcttcct aagaagcttc ttaagaaggt gttatcatct
    36661 tcttctttac ttgcatattg acgcaaccag tctaaaatcg ttagatcagt atcgaattct
    36721 ttcgttgtat cctttggtaa ataagaatta cttgttgtca caccccaacg tactgttcca
    36781 gtatctggcg taatttcacc catgataact ttgaatagcg ttgttgttgt tatatcattt
    36841 tctgcgacga aagcgacctt gtcttctttg ctcaaattaa aagatatatc atctaaaatt
    36901 ttctttccat caatcgttac acttacattt tctacctgca ataagtcgtt tccaatctca
    36961 cgttcaggtt tgaatccaac gaatggataa cgtctagaag aaggttggat atcgtctaaa
    37021 gtaatttttt caagcatttt tttacgtgaa gttgcttgtt ttgattttga tgcattcgca
    37081 ctaaagcggg caataaattc ttggagttct ttgatttgtt cttctttttt ggcatttgcg
    37141 tttgcttgaa gtttagccgc tagttgacta gattccaacc aaaaatcata atttcctaca
    37201 taaagtttga ttttaccaaa atctagatcc gccatatgtg tacatacttt gttcaaaaag
    37261 tgacggtcat gggatacaac gatcacagta ttttcaaaat tgatcaaaaa ttcttccagc
    37321 caattgatcg attggatatc cagaccgttt gttggttcgt ctagcaaaag aacatcaggt
    37381 ttaccaaata gtgtttgtgc taataacact tttacctttt gtccaccagt taattcactc
    37441 atttgctgat ggtgcaattc ttcagggatg ttcagacctt gcagtaaaac agctgcttct
    37501 ggttcagctt cccatccatt aagctcagca aattctcctt ctaattcagc tgctcgaata
    37561 ccatcttcgt cagtgaaatc ctctttcatg taaatcgcat ctttttcttt cagcacttca
    37621 tacagacgct tgtgtcccat gatgactgtt tcaagtacag tatagtcttc atagtcgtaa
    37681 tggttctgtt tcaatacggc catccgttca tctggtccca tagtaacgga acctgtggaa
    37741 ggctgcagat cccctgataa cactttaagg aacgttgatt tacctgcacc atttgcacca
    37801 atcaagccat agcagtttcc gggggtaaat ttaatattca catcatcaaa tagtttgcgg
    37861 tctgaaaagt gcaaactcac atcatttaca gtaatcaatt tttttcctca cttttatcat
    37921 tattagtttt gacaagtctt atagaattat ataggggaaa ggtaaaagat tcaaggcaat
    37981 cgaacattct ttgtgtttca cgtgaaacag aatgaaaaaa ataaaaataa aagcggagcg
    38041 aaaatagcta ctataggatt tattagtttg caacagcata atttttgacg ttatttataa
    38101 gagggagtac tataaaactg taaaagaaat gaaagaagga ttgaaaatgg atgaaaaaag
    38161 aaccatcgac tggggctcat tgattttggg aattttattt gtacttgtct cattgttatc
    38221 atttcaagac cctgtcggaa atttagttgc aattgtagta gtctttgcga tttttgcctt
    38281 tattaaaggg atatttgaac tgtttgtacg gaacagatta aaagagctaa caggatataa
    38341 aggaaagatg cccttaataa tcgggatcat cgatattcta gttggcgtct ttttcttatt
    38401 caatattggt gcaggagtcg cagcattgcc ttttgttttt gcagtatggt ttattgctga
    38461 ttcagtttta gcgttattta ctgctgactt ggcaagagga gtaagtgaag gatattattg
    38521 gtttacgatc atcgtcaata ttttaggaat tttattagga attttcttat tgtttaatcc
    38581 aatttcttct gcgttgacat taagcttttt agttggattc tattttatgt tatttggtat
    38641 cacacatatc gtctatgcat ttagataagt aaaatcaaac aaataaaaaa gccggcttcc
    38701 gattacagtt ttactgttgt aattggaagc cggctttttt atcatggaca aatatgcacg
    38761 aggtgatttt taggtgtcga gacaaattta tgcaaaaatg tgagaagcta tatacacaaa
    38821 ttttatctta atactcgaag catagcgcag ttgtcacaac ctcttttcat ataattcatt
    38881 caatactggc aaatcatgga cttcatattt ttctttgaat gcttggattt cttcttcaga
    38941 atattttctt ggatctaaat gcagatcttc tactggcaat gggcgcttgt tattgatctt
    39001 cacatcgatt actacaggac gatctgattt tgcagctgca tcaaaggcaa atcgaagttg
    39061 cgagtattct gtgactgtaa agctatttgc cccgagcgct tctccaactt taccgaaatc
    39121 tgcatcttct aaaaatacac caaatttctt ttgttccgta tcttcttgtt ctgcttcgat
    39181 gaaaccaaaa gaatcattcg aaaagacaac attaataatt ggaagctgat atttgacttg
    39241 tgtaatgata tcttgcatga ccatagcaaa cccgccatca ccacttaagg taaatacttg
    39301 tttttctggg tagcttagtt gtgcggcaat tcctcctggc acaccattac ccatagttgc
    39361 gaaccatcca gatgtcgtat gtttttgttt accgttcata ttcaataaac gaatagaatg
    39421 gatagtcgtg ttaccgacat ctgtgacgaa aatcgcatcg tcttcagcaa tacgattgat
    39481 ttctttatag acagcctctg gacgaatcgg ttcttcctct cgatcttcaa aagatttcaa
    39541 ccaatttacc catttttctt tattttcttg atttgctagg taccagctat cagcagggcg
    39601 agcatcccct aactcgacaa gtctttgcaa agctgtgttt gcatcaccga ggatcgacaa
    39661 agatgtttca tggcgtcgtc caaattttga cgcatcgata tcgatttgga taaattcagc
    39721 agttggatta aaaaatgcac gtccaaatgg gaagtcgcta ccgacaaata agatcagatc
    39781 ggcttctgcc aatgcttcat ttccgggttt agtagcgaca cgtccagcaa aaccaagaaa
    39841 gttttcataa tcatcaggaa taattccttt tgctagaacg gaagcgacaa caggaactga
    39901 aaaatgttct gagaattctt tgatttgcgg aaatccgttt cgcgttcctt gaccaacata
    39961 aagaactggt ttttttgctt tttcaagata aggcaaagca gctagcaaat ctttttcttc
    40021 tggtaatatc acacttgttt tgtgagtgtg agcggtggag aaaggttgtt cgtcaatttc
    40081 ttcaaaacca agatcaacag ggatagtcac gatagccacg cctttatgct cataggcggc
    40141 tttaatggct tcatctacta catgcggaag actttgtggt gtcatgactg tacgattgta
    40201 tacgctaaca tctgcgaaaa taggattttc atttagttcc tggaaagagt tatagttcat
    40261 ggatgtagaa gcaacttgtc ctaagaacgc tactagaggc acatggtcca tctgtgcatc
    40321 atacaaacca ttgatcaaat gtgtcgcacc aggtcctgca gagccgaaga cagcgccgac
    40381 tttccccgtt aattttgcat ctgcagcggc tgcgagtgcg ccgacttctt catgacgaac
    40441 ttgaatgtag ttgatttcgg ttttttcttg atatagcgca tccataattg aattgaaaga
    40501 tcctccggga atgccgtaaa tatggtcgat accccagctt tcaagaactt ttaccattgc
    40561 gattcctgca ttgatttttg acatggtgtt tcactccttt agcaatgatg atacataaat
    40621 tttttgtatc tagagtatag aactttttat cgaaaatccc aactaattcg tcttttcaca
    40681 aaaatgtagt catgtttttg ggaatatgct agagtagtac atagatagat tcaagagagg
    40741 ggaatggcat ggttgaatgg ctattgagac ttgaaccttg gcagcaagca cttgctggga
    40801 cagcttttac ttattttatg acagctcttg gtgccgggtt ggtttttttc tttaaagaaa
    40861 tcaaaaaaga agtgctaaac ttgatgttag gatttgcttc tggtgtcatg attgctgcaa
    40921 gtttttggtc tttgttagat ccggctatta caaaagcaga agaaaatgga gatattgctt
    40981 ggctagttgt aagtattggg tttggtttag gcggtgtgtt tttatatatg gcagataaaa
    41041 cactgcccca tatgcatttt ggaccacaac atgaagcaga aggattgcct acccatttaa
    41101 aacggacgat cttgcttgtt ttttctatta cattgcataa cattcctgaa ggtttagccg
    41161 tgggggtagc attcggtgca gcagcaactg cggataatcc gacagccgct atattagccg
    41221 ctgtttcggt ggcgctaggt attggtatcc aaaacttccc agaaggagca gctgtatcaa
    41281 tcccgttgcg acaagaaggt ctgagtcgta aaaaggcctt tgtttacgga caagcttctg
    41341 gaatcgttga accaattgct ggagtgattg gagcgctact agtaacgaaa gtggagctgc
    41401 tgcttcctta tgcacttgct tttgccgcag gagcaatgat ttatgtcgtg gtggaagaat
    41461 tgatccctga agcacagcag acattgtcca gcaaacgtca ttttgcagtt tttggcgtca
    41521 tgagtggatt tatcattatg atgattttgg atgtggcatt aggataaata aaaaaaatat
    41581 atatgatata tcaaataggg ccgcgaactt atatcgcgac cctatttttt tagtaagtta
    41641 tatcataatg aagtgaatgg tatcatacat taataatagt tttttctatg tgtatttcct
    41701 ttgatccagc gaacaagcca gatgataagt gcgattggga ttagaacttt gaccgcaaac
    41761 cataaaagcc cgcttaggac actgacaaaa agactgatga cgaagctagc tgcaattagc
    41821 actaaaagaa ccatgaagat atttaagaac atattttttt gacgcataca aaaccctcct
    41881 cttgttcatt ctttactata gaaaaagtat aacagggatt gtttaaaaac acctccaact
    41941 gaagactgat ttttacttca tctacttaaa tgatcaaaca attgcttttt ctctggttta
    42001 tcatctaaaa tagagcggaa aaggaggaga atatcatgcc taaaaatcgt cttgaagcat
    42061 ttacagatgc tgtaatcgcg attatcatga ctattttagt tttagaactt catgaaccta
    42121 acggagacac gtgggaggcg ctcgctgcat taggttaccg attttttatc tatattgtca
    42181 gttttatcat tttagcgatt tattggaaca atcatcatca tttatttcaa gttgtacata
    42241 agataaatgg acgggtctta tgggcgaaca gtttctttgt ttttgcgctt tcgctttttc
    42301 cgtttgcaac ttcatggatc agtgagtaca tcaatagcct ggctccagaa atgacttatg
    42361 gttttgttac cttgaccgct aatattgcgt attatatctt ggcaaaagaa cttgtcaaag
    42421 caaataaagg ccacgagaaa gcattgaagg cgatgttgaa taattacaag aaatcgtatc
    42481 tatcgattgg tttgaatgtg ttaggaattg tcttagggta tttagtagcg ccagtagccg
    42541 tgctgatcgc aaacatcgtg attctagctg cttggatcat cccaagtcgt accatcgaat
    42601 cacaatatcg gtaaaatgat ccgtgtttaa aatttaagag aaaaatatag aaaaaataaa
    42661 taaacgatca aatatccgag tgatactagt attcaaagag ggatgcagat ggatcaaaaa
    42721 agaattcatg taggactgat tactagtttt gtcacgatgc ttatttattt ggatcaaggt
    42781 catttgactt ggacagtagt tggtgtcgta ggatttttga cattattcac agcaataaaa
    42841 aaataaacat ctttatgtaa tctgatgttc tatctattta agaaaagcat gtttacagca
    42901 gtatgattct gttgtaaacg tgtttttatt tgtggaaaat ttaattttta gaaaagtgta
    42961 tcgaaaaata gatagttagt ttttccacag tggataactt cgtaagagat aaaaataaaa
    43021 aattagtcaa agaagggcgt atagatttct tttgatagga gatatacaac agaaatgaat
    43081 aaatattaac gtgttttcaa acagtataaa acggtgtggt ttttggaata aaaaaataac
    43141 atatctcatt ttacactttt gttattttat acttaaaaaa taacgacaca gaaaataccg
    43201 tgtctcagat ttctctacta cttctaaaaa ctactgtttt tttcctttga aatgccttca
    43261 aaaagtagga tcaggcagaa tatactggca gaatcaagga gaagggagcc taaataagga
    43321 gaaaaaccat agaaaatata aagtaattac ttataaaaca agctttttat agaaaaataa
    43381 tatacctgag tagggtataa attaagacgg aattcctttc tagattgaga tattgactag
    43441 gaagaatgtt aagattttaa gcctttaagc taaaaaaaaa ccctttttgt taaataaggg
    43501 gaatatagcc tgtcagtttc aaactgcaag tcgtcctgct gcaataatat agtagattag
    43561 agatgaaaat agattttttt aaaccaatga ctgaataaaa agaccgtcat agatacacat
    43621 tggaatgtgt ctattacggt ctttttttac gaacgatttt attgaagacg gacagttcgg
    43681 gtcaaaaata aaaaagctac gatcagaaat aagaataaaa tgtatcccaa aataatggct
    43741 agacggtcta acttatttgt cgatagatgt ttgtttagaa gttgataggc ccataaacct
    43801 atcagtcctc ctgttgtatt agttatcaaa tcagtgatgt ccgctacgcc aataccgaat
    43861 aggaattgaa tcaattctgc caagaagctt acaaggaaaa taatccctaa ttttttagaa
    43921 aaagaaagtc tcttgaaatt gacattcaaa agcaccccaa aaggaaaaaa gacaatcaag
    43981 ttataagtgg tctcacgtaa taccgtcgta ttttcaaaag aaaaagtaga aaaagggacg
    44041 agattgacat tgctgtaaga ataagtgaga aattgtggaa gattaaatga tagcttgaat
    44101 aaaatcaacc ataatagaaa gaataaataa atccctaata aaaattttga tacataccgc
    44161 atagacatct cctccattta aaaagtatac catcgaaaac cgctgaaaaa tcataaaaaa
    44221 gtatgaagtt taaggaccaa cgtagttatc gtacgaaaat tagaagcttt tttttgttgg
    44281 gaatccggtt tctttaacaa agctaagata gcgagttgct tggaaaagtt gctatctttt
    44341 ttctgtcaaa acgactcgga atgttcgttt tttgttctaa tttctccaag ttgtttactt
    44401 tttgatacta agtttatgaa aaatcaagaa atagttgtta tactaggtaa tgctgacaaa
    44461 cgggattggc atttcatctt ggttcagctt tttttccaat tactaggaag gagggataga
    44521 ttgttcaaga cttattttcg tttcaataaa atacaagata atccttttcg tatcatgaat
    44581 ctgatgctac ttatctttat tgtattatgg ttaggttaca taaatcatga ttttttgatt
    44641 tcaagttttt catcattagg gatctttact tttttctatt atcaaaacat accgatgaaa
    44701 tatttgctga aacgtatgtt tctgatcggt tgcggactgt tggttgcttt cgttttgggg
    44761 atcttatcga cttatgtttt atggcttgaa ccatttgccg tagctttggt tgctttttcc
    44821 agcagattcg tgttgcgatt attccatatt tctaaaccag gtggcttgtt ttttgcgatg
    44881 ctttctgcca tgggaacgag catgcagctg ccaatcgctc aattaccgat cgtctcactt
    44941 tattttttta tgggtgtcgt ttttgccctg attgcggcag tgattacaaa attactggat
    45001 tcacggcctg agcagacaat tgaaaaagca actttaaaag aaagatttca cgaagagcct
    45061 ttagtgatca ttgattctgt tttctacagc gctgccttat ttctttccgt ttatgtcagt
    45121 cacggcttga atctgcataa tccttattgg ttgaccttgt catgtgcatc gattttgcta
    45181 gcagaaaact tggatgcgat gaagcatcga caagtccaat atctgattgg atcgatgggt
    45241 gggttatgtg tgtcggcatt cttgtcattt gtgccgttta cgcagttgca gaccattttt
    45301 ctgatcactt ttctatatgg aatcgcacaa tttctagttg ctagaaatta tgcagttgct
    45361 aatatttttc ttaatctgat ggcgttgatg ctatctacac ttattcgtgg agcatacttg
    45421 atttctttga tcgaatatcg ttttttaggg atcgtcatag gaagtttcat cggtttaggt
    45481 gttgcgtggg taatgacggt tggtttgcaa cattatttaa cggttgttcg tcaaaaatta
    45541 gaatagaagg acaaatatat gtgccaaaag aggttatgac aagatttttg tcacaacctc
    45601 taattcaagg tgttcaaaag ccagattctc agcattaagt caaattcaca caaaaatgtg
    45661 gaaaggaact ttcaaaaaaa ttttatattt attctatcaa aaaattttgt ttatgatggt
    45721 aactgaataa actttcctga gatggtcaat gctttccttg aaagtcttac agatattctt
    45781 tcttcataga aagtttaagt tttatagtta gtgtacaata aaagtatgtt tataccatat
    45841 ggtgtccgga taatcagtca tttaaacaat gaggttcaaa gatatgaaaa ggggaaattt
    45901 gatgaagaaa ataagcaatg aaatccaaat tctggcagaa actttaactc ttcccaaagt
    45961 cgttatagag caaatgattg tacaagcaaa aaacatcctt gatgaggtgt ttgagcaata
    46021 tagtcatgaa ctaatggatc ctaataaagc aaaggcgact gcaaagaaaa tagacaaagt
    46081 ctatgaagag gaaccttttg cagcattagc tatttatcta tatgcagctt ctcaaagttg
    46141 gaaaaaaatt tatcaaccat tgaagattcc aagaactatc tatttggcaa caatgaatgc
    46201 ttttactaga tttattcatg aacatttcca agtaacagga agctatcgct ttgaccgtgg
    46261 tttttggatt tggcattaca ctagtggctt gatctttcgg attggtgaat tggagtacga
    46321 aatgacatat tttccgaaag ggtacacgga tctaagatta gaaggaaagc agtgcctttc
    46381 tcttcatatt ccttccgatg cagatttatc tcagaataag ataagagata gttacgaaag
    46441 tgccattcgt ttttttgaac tttattttcc tgattaccat taccaagtga tgtacacaga
    46501 tacttggtta ctatcaccca atcttactaa atggttgaaa aaagagtcaa aaatctgtct
    46561 ttttgcagcc gattatcgat tattatcagt tgatgaacag gacgatagtg gagtcccttg
    46621 gatctttgga cgagtagatg cccaaataca tgattatcca gaaagtacga gtttacagcg
    46681 acaagcaaaa gagcaattgc ttgcaggcga gcacattggc tctggcttag gtattcttac
    46741 ttcataagtt tagtacttcg aaatagttta aaagagcgtg agacaaaagt aaagaacact
    46801 tttgtctcac gctctaaaca cgtataaacg gcggaagcag aagcaactcc ttcggaaata
    46861 agctgaaatt cacaaaaatt tgaaaagcaa ttttcgtgaa tttcttctta tttcttggag
    46921 ttaaacgctt ctgtcccagc ctctttttta gtgttctatc gaacacacag tacgttcaat
    46981 aggtaaacta gctataacaa tagatattaa agttaacgga caagagggcc ttttttaggt
    47041 agctaaaacg taataatgaa aatttatttc cagagatagg agtcaatcag ctagtctgat
    47101 cgactgtttt aacaagttga ctttcatttt aacttttgat accatttaaa acacaaatcg
    47161 taattgtttc gatttgtgtt ttttagcatg actacaatca aagaagaaag tgggtcgtcc
    47221 atatggaaaa aacagatatc tcaagtgcat accgccgctt gaaaagccca aatatcaaaa
    47281 cacgaaaacg tgcgctgaag atcatcaaag atgtgaaacg gaatagtggg aaaaggtaag
    47341 aagaaggaaa cttaattaaa aaaatagccg aaaaacgttt ttcggctatt ttcaataatc
    47401 ggtttttgtg taaggtttcc gaaagaactg ctgttgattt tccattttta acatatgata
    47461 aattggtaga atctttcttt tcaaattgat accattccct aattccagtt atatagttaa
    47521 ctacagaacc acaaagcacg tacgtttata aaagtattat ctgtagttat ttttcaattc
    47581 cctttcaacc tcttattttg accagtagta ttgatcactt taccttttaa cacctttgtt
    47641 cgtttttgaa acatttttag tcaagaatgg aaacgtaact acataatcaa aggttttgcg
    47701 aaatatttca tgaaatattt catgtagaag cgagcaatac ggttgcaaat aaccattatc
    47761 gtttaataag cagttacgaa agctcccaat atctctaacg ttcagctcac tccatttctt
    47821 ttcttcaaaa aaatcagtga ttcgtatctc tttctatttg tcgaaaaagt taactttaaa
    47881 aagtaacgcc gaaaaaatat gcaaaaaaat aaaaaagcag ttactattgc tagaataaca
    47941 tcaaggaata ataagttttg gttttcagat aatagtcgat aagttgtgta ttggaatttt
    48001 agatacagta ataaagtatt tttcatgaaa ttttgtgaga atcactatta caagtatctt
    48061 taataaaatt aatggttaag gaggaactta aagtagaatg taaatgaata gttgtagtca
    48121 gtcgattgtg atactagact caaagttgca ggaataagtt tttcaatttg tgattggaag
    48181 ataaaaaatg aaaaagaagg gctttttttt atactttgtt gattgtagtg tttattttcc
    48241 ctctgtttag ccaagtaaca ttttctatga ctcaaccagt ttcaagtgag gaacatgata
    48301 acctttttcg tgctacagaa gagtttaaga aatatcagga aaatattgtt ccggagactc
    48361 cattagctag tgagccaatt actgatattg atggaaacca aataggatgg tatatacagt
    48421 acgaagttat ggtggtaaat aatgactcaa tagtaaaaaa tacagtaggc ttagagctaa
    48481 caccgattga aaaggtgagt tcattgttaa cgtatattta tactgatgaa agtgacgaga
    48541 ctgagatcat tttaacggat tactcaaaaa ttgctactga taaaaagtat atattgtgga
    48601 tgtagacact agtgaaatac tttatgatat agaaattgat gaaaatttac tagatgtagc
    48661 acaagaaatc gatcatcaaa agcaggaagc tattcgatat gctggtagaa aagggaaaag
    48721 aaaatattta ttacgtgaat gtagcaaaag ttagagaaga tgaaaatgag tggaaagaat
    48781 tcaagagtcg ctactcaatt aactcgactc cgacatttac agtttacaga gaaggatcga
    48841 tagaaaaaac tgttttttgg actaaagaaa gtggaatgtc tttagctgaa gtagaagagt
    48901 ttttggatta tgttagtatg caacagtaag ggagttctta ttatgaaaaa ttttgacaca
    48961 tattatgtta ttgttttttc gatatctttg ctgttaatat tactaacttt aatgcagtat
    49021 aaagagtatc cagagtattt tattttaact tttgtatatt tagtaagtag ctacacactt
    49081 ctatataaat tcagaagttc ttctagagtt tctaaagttt gtttcttaat tagcttattg
    49141 attctataat atttttgatt tatataggaa attaaaaata ttatagaatc aacactcaga
    49201 gaacgggttg gtttttaata gatttaataa gtactgataa tgaaatttct cctggttggg
    49261 aaaacgaagt tggttaccct gtagaagaat tttttcaaga ttactttgcg gagtatgccg
    49321 ataggataat gagagattac gtaacagata aaactcaaga agtcatcaat caatcccagt
    49381 cattaaatga attatctgat aaggcgaaag aagaagcagt tgccattatt tcttcaggga
    49441 ttagtgacta tattgatatg agtggtgaaa tcaattatgg cgataaagac aaaattgtac
    49501 gcatgataaa agcttttatc aaaacttttg atgtgaaggc agatcttaag tttaatgata
    49561 cttacctagt aggaaaatta attaatatcc ttgggacaga gaaagatacg gaaacgttta
    49621 tttcctcttt gtctatgttg tttacagata agcatttttc aactcagctt ggtggagagg
    49681 aactagtgga aatcttttaa ccattacgcc cagcattaat aaaagtatat caaaaaagta
    49741 ctcctgatga tttttaagat tggtttgaga aataatgtga aaacactatt tttaatgtta
    49801 attatagata gagatgaacg cgtcatatgg ttaaaagaat gaaaaagtca aagatacact
    49861 gctatatttc actaaggata tagcagtgta tctttcatgt cgtttcactg gcagtttttt
    49921 ataaaaggag gtgtgcattt cagttgcttt tctttggtag ttagttgaat cgtttaagaa
    49981 ttaacggatg tggcagacag gtgctctgtc caaaaaccag taaaatcttt gatggttcca
    50041 tctttattta gctgtgcctg ttcaaatact gttttgtttt taccgaatgg atcaattgca
    50101 ataattgttc cgatccccgc acgtttggca gcaagtaatc ctgagtaagc atcttcgata
    50161 acaagacaat cagccggagc taagctcagt ttgtctgccg ctttaagaaa aatatccggt
    50221 tgcggtttcc cagggaaaga gccgtcgtca tagacaatct tgtcccagtc aaaccagcgg
    50281 gctaaatcga aaatatcaaa ataaaaagca acattctctt tgacagtagc cgtggcaatc
    50341 gttaatggaa tctgttggtc agtcagctgg tcaagagtcg cttcaagccc ttttgtcaac
    50401 tgaagctcgt tttcattttg caagcaaagc atacgataat agctttcttt ttcttctgac
    50461 aatacggcga tctcagaggc ggtcaaatgc tctgaaatga aatgtttcaa aatctccgta
    50521 ttcgtccgac catgaatatt ttttaaaata tcctcgtctg ataaaccatc ttttgtatga
    50581 tcatgtatca tatgcagcca agcagcttca tgcagatgag agtccaaaaa cattgttccg
    50641 ttgaaatcaa atataatccc tttcatagtt acgctcctat agtggtattt tcggttgaat
    50701 cagcaatttg cagatcgaca tattgtgcat agtgctcttg aacctcccgc gagatctggc
    50761 gatccgtaat gagcagatcg aaatcatgca aattataaaa cacataaaaa tcatctcgat
    50821 tgaatttttt attatcaacg agtagatatt tcttacggga attatttaga gcgatctgct
    50881 gtgcttctcc ttcttcggta ctgtaagtag agatcgcttc gttgtgaacg ccatttgaac
    50941 tgataaatgc ttttgtaaac tttagtcttc ttaaattgtc gttggtgatc ggtccgacaa
    51001 atgcgccggt atttttccgg aaatcaccgc caatcagtag aatttctggt ccatctgctt
    51061 gagcaagaat atcaaaaact ggataattat ttgtaatgat ccgaattttc ttgttcagta
    51121 aatgcgtcgc caatagttcg attgttgtac ctggtcctaa gaaaatcgtt tctccctcgt
    51181 gaatcaagga ggctgctgtg gcagcaattt cttttttttc ttgaatctgc agagtggctt
    51241 tttcagtgtg agaaagttct tgatctaaag tgtaggaaag actttgagcc cctccatgga
    51301 cacgcagcag cttgcccgat ttttctaatt catctaaatc tctgcgaacg gtcatatcag
    51361 aaacatctaa ttgattgatg atttcattaa cggtaaggat tcctttttta ttgaccattt
    51421 ctacgatagt aagcaatctt tcttttttca acattgtctt cacctctaat ttaaggataa
    51481 tctatcatat aaacggacaa cagtcaatct aattcgaaca aaagtaaaca tttagggtgg
    51541 tttttatgtt tgttacaata gattttattg ctgttttctg gtttttatga ttatttgtgt
    51601 ttgtttttgt tgacaaatgt tttggatgcg tttacaatgc aatcagttag aaagagcgaa
    51661 aactgattat agatttgagg agagatgtac atgcttaaat taccagaaga ttttattttt
    51721 ggcggagcca cagcagccta tcaggttgaa ggagccacta aggaaggtgg caaaggcgct
    51781 gttgcttggg atgacttttt agaggaacaa gggcgattca gtcccgatcc ggcaagtgat
    51841 ttttatcatc aatatgcaaa ggacattgaa ttgtgtgaac gttttggtgt aaatggtcta
    51901 agattatcca ttgcttggag ccgcattttt cctgatggag ctgggaaacc aaatccagaa
    51961 ggaatcgctt tttatcatcg tgtgttcgaa gaatgtaaga aaagaaacgt aacgccgttt
    52021 gtgacgttgc atcattttga tacccctaaa cgtttgtttg accatggaga ttttttgaat
    52081 cgagaaacaa tagaagcatt tgtttcctat gccatttttt gttttcatga attcaaagaa
    52141 gtcaaggtgt ggagtacctt taatgaaatc tatccagttg caaccaatca atatttgtta
    52201 ggtgttttcc caccaggaat caaatatgat tttacaaaga tcgtcgcctg tcttcataat
    52261 atgatggttg ctcatgcccg tgtagtgaac tatttcaaag aaaacgaact tccgggtgaa
    52321 attggtgtag ttcactcatt agagacgaaa tatgcggcaa cagacgcgcc ggaagataag
    52381 catgctgcct ttttagacga tgcattgtcg atccgtttct tattagatgc tacctattta
    52441 ggctattatt caactgaaac cttgactgct cttgatgaga tatgtgaagc aaatcaggcc
    52501 agctatcatt ttcctgaaga agatttcgtt gaattgaaaa aagcatccac gcgtaatgac
    52561 tacttaggga tcaatcacta ccaatgccat tttgtcaaag cttacgatgg agaaaatgca
    52621 atccatcata acggtacggg tgaaaaaggg acctctgttt ataaagtgaa gggaatcgga
    52681 gaacgtattt ataaagaagg aattcctcga actgattggg actggctgat ttatccagaa
    52741 ggtctatatg acttgttgct gcgaattaaa agcgattacc cacattacaa caaaatctat
    52801 atcactgaga acgggatggg gtataaagat cagtttgaag atggcattat tatggatcag
    52861 ccgagaatcg actatttgag agtctatttg gaatccttaa gcaaggcgat aacagccggg
    52921 gtaaatgtga aggggtattt tttgtggtcg cttatggatc ttttctcatg gacaaatggg
    52981 tataacaaaa ggtatggctt gttttatgtt gattttgaaa cccagaagcg ttacccaaaa
    53041 gaatcagctt actggtacaa gttggtcagt gaaaccaaaa cgattattta ataagtgata
    53101 atggaggaag aaaagatgaa taaagaggaa ttacaaatgc ttggttttga gatcgttgca
    53161 tattcaggcg acgctcgcag tacattattg aaattattga aagaagtaag acaaggaaac
    53221 tttgaacatg ttgattcggc gttgaaagaa gccgacgaaa atttaaccct tgcacataat
    53281 tcgcaaacaa aaattttagc ggaagaagca tctgggaaag agatggagat gggattcatt
    53341 tttatccatg ggcaggatca tttaatgacc actttattgc tgagagatct gattcaagac
    53401 tttattgtac tgtacagaca gaatcaagga taggagaaga acgctatgga tggaatcatt
    53461 aaacaaatcg aaaaagggaa accatttttt gaaaaaatct cacgtaatag ttatttagga
    53521 gccgttcgtg acggattttt aacagcgatg ccagctattt tattttcaag cattttcatt
    53581 ttgattgctg cggttcctga gatttttggt taccaatggc ctgaatctgt ctccacatgg
    53641 ctatggaaag tctataatta ttcgatgggt gttgtagggt tactcgtttc agcaaccact
    53701 gctcgctgct tggctgattc gatgaatcgt cgcttaccga aaaacaaagt aatcaacacc
    53761 acctctgtga tgctggcctc tgtggtaagt ttcctgttgt taagcgtctc tccgattgaa
    53821 ggtggatttt caacggaata tatggggaca aagggactac tggcttcctt tgtcgctgca
    53881 tttttaacag ttaatttata taagttttgt gtgctgaaag atattaccat taaaatgcct
    53941 aaagaagttc ctggaacgat ttcgcaaacc ttccgagata tttttccttt ctctttttca
    54001 gtatttgcgg ccgttttaat tgatttaggc attcgacaaa tcttccaaat ctcatttgca
    54061 gaagcaatca ttacggtgct gcagccgcta tttacagcag ctgacgggta cttaggaatc
    54121 gctattatct ggggagcgat ggcgctgttt tggtttgtcg gtgttcatgg accgtcgatc
    54181 gtggagccgg caattgcggc aattatctat gccaatgtgg aaaccaatct gcaattgttc
    54241 aattccggtg aacaagcttc taaagtactg acggtgggtt tagggaattt tgtaggaact
    54301 atgggaggaa ctggggcaac attggttgtt ccttttatct tcctactctt tgctaagtct
    54361 aagcagttga aggcagttgg gaaagcttcc tttattccag taagtttcgc tgtcaacgaa
    54421 ccattgctat ttgcggcacc catgattttg aacccttatt tttttattcc atttttgtta
    54481 acacctgttg taaacgtttg cctgttcaag ttttttgtcg atgtattggg catgaacagc
    54541 tttatgtacg ttttgccttg ggcaactcca gcaccgatcg gtttggttct aggaacgggc
    54601 atgggtatct tagcttttgt tctagcagcg gttctaatcg tcgttgactt cctgatctat
    54661 ctaccatttt gcaaagcata cgatgctgtc ttagtagaga aagaagcaca aaaagcagag
    54721 gtgatcgcaa ccgaggaagg tgcttctgcc gatgtacgtg aggaagtacc tgtggcagcg
    54781 agtcaagtta tcagcgatgc aaataaagaa atcaatgtgt tggtactttg tgctggcgca
    54841 gggacaagtg cgatgttagc taatgcttta aaaaaaggtg catctgaatc gaaagcaccg
    54901 atcagtgcaa tggcgggtgc ttatggttct cattacgaaa tgctgcagga ttttgatatg
    54961 gtggtcttag caccgcaagt caattcttac tatgatgata tcaaagaaga caccgatcgc
    55021 ttaggaatca agctggttgc gacaaaagga gcagaatata tcggattaac gagagatcct
    55081 gaaaaagctg ttcagtttat tctgaaccaa tttcaataaa ggaggagcca taatgaaaat
    55141 gggaatttgg ttgcgtaaag ctgttttatt tgcagctgtg ttaggggctt tgggggctgt
    55201 ttcaaaaact gttgaagcat ctgccagcga atttattcgc ggagcagata tttcgatcct
    55261 gtcagatatg gaaaaatctg gtgcaaaata ttatgaagac gatatttcaa aagatgcctt
    55321 agagatttta aaaaataatg gtgtcaacta tgttcgactg cgtttgtggc aggatcctta
    55381 taatagtaat ggagaaagtt atggagcagg gacaaatgat ctggaaacga cgattgacct
    55441 tgcaaaacga gcaaaaaatc tgggacttaa ggtgctgctg gattttcact acagtgattt
    55501 ttgggttgat cctgggaaac agaatcttcc taaagcatgg caaggtctga cctttgaaga
    55561 aatgaatacg gcattgtacg actatacaaa gaacgttctg tcggaaatga agcagctgga
    55621 cgtttatcct gatatggttc agatcggcaa tgaactgaat agcggaatgc tctggcctta
    55681 tggcaagtct tggggagaag gcgggggaga atttgatcga ttggctgctt ttttgaagtc
    55741 tggtatccaa gctgttcgag acactcaacc taaaacaact ccagtaatgc tccacttagc
    55801 tgatggtggc gatacaggag catttacttg gtggtttgat gaaatcacga gtcgaggtgt
    55861 gtcatttgat ttaattggag tgtcgtatta tccgtattgg cacggttcaa tgaatgaact
    55921 tcaaaccaat atgaatgcga tcagccaacg gtatggtaaa gaagtgatcg tagttgaaac
    55981 agcttatggg catacaacag cgaatgccga tactatgcca aatgcttttg gtgaagccga
    56041 agcagctgcg gggggatatg aaccaacacc gacaggacaa gcagagtatt tgctggatct
    56101 tgccgatcgg attcaggcag taccgaataa tcgcggggcg ggcttcttct attgggaacc
    56161 tctttggtac aacggaaatg tgagttgggc aacacaagca ggtatgaatt atctcagtgt
    56221 tcaatccact atgggaaacg aatgggacaa ccaagcgatg tttgattttg ctggaaacgc
    56281 cttgccaagc ttgcgtgcgt ttaaacaagc aggggcgcaa ataaatctag ttaaaaatgc
    56341 aagtttcgaa agcgatggac caacgaccag tccttccaat tggcaaacct ggtttcaaca
    56401 aggaaccgct aagggtacga tcaaaacgga agcgggcact ttttttgggg actacaaact
    56461 aactttttgg caggatcaag catacgaggc atcagcttat caaaatgtta gtggcataag
    56521 caaaggaact tacacattga gtgcctatgt aatgtctggg ggagagtttg atactaatca
    56581 gttatatatc aaaaattatg gtggagcgga actaaatact gcggttgtta aaacaagtga
    56641 atggacaaaa attacaatca aagacattcc aattacaaca ggtgtttgtg aaattggggt
    56701 ttatagcaag gcttcaggaa atgcttggac gaacgttgat tttgtttcat tggtgaaaca
    56761 atagactgcg gatcgtttcg attcaagatc ggaggttccc ttcagccaca tcgttagcgt
    56821 agaaggcagg aatcgaacct gcacctcgcc tttgccaaaa ataaccagaa acgatttcca
    56881 gtcattttta ccaaaacgag taaagcacca gacttcatct acactttgta ttatgctaaa
    56941 gagagttcaa cgatttcgta agaagcaatg aaaaaattgc aaaaagttgt gcagataaca
    57001 taaagtttgt ttaagaaagg gtaaccttag aattccgtcg tgaatatgat ttgagcactt
    57061 agatggatag aaaaaagttc tcggaaaaca gttcgttttt cgagaacttc tttttatgat
    57121 aagaatgaaa caaattaact tatattatag tctcttttgg tagggaacca tcttattttt
    57181 aatgaaaatc aagcatttta gctattgaag aatgttgatt tatagggatt ccccctccat
    57241 attatgttgg ctctttattc ccactccact gttgctggtg gtttagaagt gatgtcatac
    57301 acgatacggt tgacgtgggc tacttcgttt acgatacgta cagagatttt ttgcaagact
    57361 tcccatggga tacgggcaaa gtcagcggtc attccgtcga ttgaagttac agcacggata
    57421 ccgactgtgt agtcgtaagt acgtccgtca cccataacac cgacagagcg gatccctgga
    57481 agaacagtga aatattgcca gatatcacgg tcaagtccag cagctgcgat ttcttcacgc
    57541 aagatagcat cagattcgcg tacgatttct agcttgtctt cagtgatttc gccaagaaca
    57601 cgaataccaa gaccaggacc agggaatggc tgacgccaaa cgattgaatc aggcatgcct
    57661 aactgagtcc ctaattcacg gacttcgtct ttgaataatg tgtttaatgg ttcgatcaat
    57721 tcaaattgca tatcttctgg cagtccacct acattatggt gagatttgat cgtctgtgct
    57781 gtttctgtac cagattcgat aacgtcagta tacaacgttc cttgtgctag gaaagagacg
    57841 ccttcttcac cagcaagttt tgttgcttcg tcatcaaaaa catatacgaa ttcattccca
    57901 atgattttac gtttttgttc tgggtcagat acacctttca acttgtctaa gaagcgatct
    57961 cttgcattga ctttgatgat gttcaagccg aattttccgc ctagactttc cattacttgt
    58021 tccgcttcgc ctttgcgtag cagaccgtgg tcaacaaaga tagatgtcaa ttggtcaccg
    58081 atcgcttttt gtaaaagaac cccaacgaca ctagaatcaa cgccacctga aagaccaagc
    58141 aagacttttt tgtcaccaac ttgttcacgg attttttcta cttgcatatc gataaagtta
    58201 tccattgtcc agttaccttc acagtgacaa acatcaaagg caaagtggcg cagcaattcg
    58261 attccgtatt cggaatgacg tacttctgca tggaattgga taccataaaa attacgtgtt
    58321 gtgtcttgga tagaagcaat cggacaatct ttgcttgttc cgactgtttc aaagccttca
    58381 ggaactttag ttaccaagtc accatggctc atccatactg tttgttgttt tggtgtttct
    58441 ttaaacaaga cagcttgatc gcttgttact tctaacaagg ctttcccata ttcgcggttt
    58501 ttagcaggct ctacggaacc gccaagtttg tacgtgatca attgcatacc atagcaaatc
    58561 cctaatacag gaatacctaa attgaagatc tcaggatcga tcccaaatgc attctcatca
    58621 tatacactgt ttggtccgcc agaaagaatg attccctttg gattcatcgc ttttacttct
    58681 tctgcagtaa tgcggtggct caataattcg gaaaagactc caagatcacg aatacgtctt
    58741 gtgatcaatt ggttatactg actaccgtag tctaatacga taatcttttc aacgttcgtc
    58801 atatcggcaa cgtttgtcaa atttattacc cctatccctt taaattttat ctcaaaatag
    58861 tcaaactatt ttaaaatgct aatattttcg catgaaaatc gaatcgatga tatgattttc
    58921 agttttatgg agaattatat cggctcttcc tcgtgtaggc aagatatatt cctgtaaatt
    58981 tttcaaattc acatttttcc atacatcttt tgccatttta aaggcttctt cacgatctcc
    59041 gatagcaaaa cgataataat aattctctgg attttggaaa gccgtatcca gtaacgcacc
    59101 aaaacgatcc aaataccatt tttcgatcag cacgggatcg gcatcgacat agacagaaaa
    59161 atcaaaaaag tcgctgacgt agatttgctg attcgcaggt aattgaagcg tattgatccc
    59221 ctcaacgatc aagatatcag gctgctggat caattcgtac ttgtctggaa tgatatcata
    59281 cacatcatgt gagtaaacag gtacttttat ctcttcctga ccagatttta cacggttcaa
    59341 gaaatcgatc agcatctcca tatcataact ttcaggaaat cctttacggt ccatgattcc
    59401 acgctctttt aatatacgat ttggataaag aaatccgtca gtcgtgatca gctgaacgtt
    59461 tcttcgtttg aaggtacgag acaaaatcgt ctgcagcagt ctggctgttg tactttttcc
    59521 aacggctaca cttccggcga tcccgataat aaaaggcggt acttctacgt atttatgaag
    59581 aaataaacct ttactcaaga acaaagattc aaattctttc atataaagat aaatcagatg
    59641 agcaagaggt acatagatat ctcgaacatc ctgcatggaa atgcggtcat tcaaactttt
    59701 gatattatct agttcatctt gtgtcaaagg tgcttgccca ttacgataaa attctcgcca
    59761 ttcttctctt gccacacggt agtagttcat tggatcattc atattagcct cctgcgttct
    59821 cccatcaatg gaactatttt aacacaaaat aaacgcaaac cccaccactt aagagagatt
    59881 gccgatcttt ttgtataatc tttataatag aaagaaacgg agtcttattc ttgacttgcg
    59941 cacggataca taaaaggata gactgttgtc atgaaagaaa tgagaaggat ggaagacatg
    60001 acaaatcatt actattctga acgcccagat acggcgcacg attttgaaca atgggctttt
    60061 gagttaaaag gcagaacctt tcaatttgta acagattctg gtgtattttc acgtgaaaca
    60121 gtggattatg gttcacgtgt cctgattgat gctttcgatt gggaagagtt acctgatgaa
    60181 ggaaagatct tagatgtggg atgcgggtat ggaccgattg ggttagcttt ggcatttgcg
    60241 acacagcggt ttgttgaaat ggtcgacatt aatgaacgag cggtaagtct agcgcaagga
    60301 aacgcaaagc gtaaccaaat cgaacatgtt gatatccatc aatcgaatat ctatgaagca
    60361 gttcatgaag aaacctatgc agctatcgta agcaatccgc cgatccgtgc tggaaaaaaa
    60421 gttgtacatg aaattttgac aggcgcatat cctcggttga agaagggcgg tacactgaca
    60481 attgtgatcc agaagaaaca aggagcgccg agtgcgcaaa agaaaatgga agaaacattt
    60541 ggtaatgcag aaatcgtcac aaaagataaa ggatattata tcatcaaaag tgtgaaagaa
    60601 taactagctg aaaaatagtg gagcgacgaa agagtggaac tgggcgtgca tgtacgggta
    60661 cgtatcagtt ctttttttgt tgagaagttt ttaaaaacaa agaactattt tttcttcaaa
    60721 aagaaatagt aggaacagag tcgttgtttt ttacgataaa ataaataaga tacagaactt
    60781 gtatagaagg ctgccaagtc ggtagataaa ggaggttttt atcaaaaaac atactttcct
    60841 tttttctcta ttcttttctt gggggttttg gtacactgat agggaattta gatagaaggg
    60901 agccaatagg atgaagctga ccatcagaga tatcgcagaa atggcaggcg tatcagtaac
    60961 aactgtctca cagattttga ataacaaagg aagcagattt agtgataaga caagaaaaaa
    61021 agtgttgtcg atcgtgaatg aacatcatta taagcccgat tatttcgctt caaatgtgat
    61081 caatcgacat tcaaagacca tcggaatgat cgtccctgat gtcactgatt tcttcttctc
    61141 caaactgatc gaaggtgtag agagctattt gaatcctttg ggctatgtga tcatgttatg
    61201 caattctcgt catagccaag aaaatgagat caagtattta caagagcttt cccaccgctc
    61261 agtggacgga atcttgctgg ccacaccaaa tattttacct gagaaatatg cattagacag
    61321 cggtttttat caaaaaatgc caatcatttt aatcgatcgc ggattgaatc gtcgggataa
    61381 cggacggctt atcgtaaagg aatatgaggg cgcctatcaa gctgtttcgc tgtttattga
    61441 aaatggccat acgaagatcg gcatgctgaa ggaaaccact ggatactatc aattagaaga
    61501 acgtttcaac ggatatcgtc atgcgctgaa agatcatggc ctgcctttta atgggaaatt
    61561 tgttgaacat ggagagttga ctgtccaagg tggatatact gcatcaaaaa aattactcaa
    61621 acataaagat atcacggcta ttttttgcgg gaatgatgcc atggcgatcg gatgctatca
    61681 ggcgatcgat gaattgggca aaaaaatccc agaagatatc tcagtcatag gattcgatgg
    61741 attaaagctg tcagaataca tgattccgca attgacgaca gtgcagcagc caagttttga
    61801 tattggattt tatgctgcac gtttcctgat cgatacgatc gaatttcctc aaagaaaagt
    61861 tccgaacaaa gtgttcgaaa caaaattaat tattcgtgag agcgtaaaag tattgacaaa
    61921 gggctaactc ggcggtatat tgcttatgta agcatttaca aaggctagtt agtgcttttt
    61981 ttatttttag taaaccagtt tactaaagtg gtttaccttt cctaaaaata atgtataata
    62041 ccgaatgaag acaaggagga tcaggcaatg cgaatggtgg atctgattga aaaaaagagg
    62101 gatggaaacg agctgtcgaa agaggaaatc gagtacatcg tcacgaacta tacgaatgga
    62161 aagattccag attatcaagt cagtgcatta ttgatggcaa ttttttatca agacatgaca
    62221 aatgaagaaa tcacgaactt aacattggcg attgctaatt ctggtgatgt catcgatttg
    62281 agttctcttg aaggaataaa ggtagataag cattcgacag gtggcgttgg tgacacgacc
    62341 acattgattt tagcccccct agtagctagt gtcggagtga cagtggctaa aatgtctgga
    62401 cgcggtctag gttacacagg tggtacacta gacaagctag aagcaattcc cggatttcag
    62461 atcgagcttt cggatgaaga atttgtacgc attgttaatg aaagtaaagt agcggtaatt
    62521 ggtcaatcag gaaacttggc accggcggac aaaaaattgt atgccttgcg tgacgtcaca
    62581 gcgaccgttg attctcttcc gctgattgcc agttcgatta tgagtaaaaa aatcgcggca
    62641 ggagcagatg caatcgtttt ggatgtgacc actggagacg gtgctttcat gaagaacatc
    62701 gaagatgcaa gacgtctggc aaagaccatg acgagcattg ggaaactggc taaccgagaa
    62761 acagtggcgg ttatctcaga tatgtcagaa cctcttggcg aagctatcgg aaatagtcta
    62821 gaagttgtcg aggcaatcga aacattacaa ggaaatggtc cagaagatct agtagaaatg
    62881 tgctacgcct taggaagcca aatggttgtt cttgctggca aagctaagac aaccgatgaa
    62941 gcaagaacgt tgcttcaaga agctctggaa tcaggtaaag cgttagcaaa attcaaagaa
    63001 atgatccaaa atcaaggggg agacccagcg atcgtggaac atccagaacg aattttgact
    63061 gcacgctaca caatggagct tcctgctaaa caaagcggag ttgtgtcaaa aatcgtggca
    63121 aatgaattag gaattgctgc aatgatgcta ggtgctgggc gtaaaaccaa agaagaagat
    63181 attgatcacg ctgttggatt gaaactgcat aaaaaaattg gtgacactgt gacaaaaggt
    63241 gagtcactat tgacgatcta tagtaacgac gaagaaatca cttctgtcat tgaattatta
    63301 tataaaaata ttgaaattgg tgaatcggca atgaagccaa cgttgattca tgacatcatc
    63361 acagaataaa ggaaaaaagc ggactttacc gttttaaaga aaaggggaaa agaaatggaa
    63421 ctaaaccgaa tgatcgacca tactattttg aaagcagacg catctaaaga agctgtgatg
    63481 caaattatcg aagaagcaaa aaaataccat ttttattctg tctgcatcaa tccagcatgg
    63541 gttgcgctag cgaaagaaca attacaaggt gagccagtag ccgtatgtac agtaatcggg
    63601 tttcctctag gagcaaacac acccgagaca aaagcatttg aaacaacaga tgccatcaat
    63661 aacggtgcag atgaagtcga tatggtcatc aatatcggtg ctttgaaatc aaaggatgat
    63721 caacaagtcc aaaaagatat cgaagcagtt gttgaagcag caaaagatcg tgcgcttgtc
    63781 aaagttatta ttgaaacatc gttattagat agagaagaaa tcatccgagc ttgcgaaata
    63841 gcaaaagctg caggcgctga ttttgtcaaa acatcaactg gtttctcaac aggcggagca
    63901 aaggtagagg atgtccgttt gatgcgtgaa actgtaggac cagaaatggg tgtcaaagct
    63961 tccggcggta tccataacga agaagaagca atggcaatga tcgaagctgg tgctacaaga
    64021 atcggaacaa gcgcaggagt agcaatcgta tctggttcaa ctggtgaagg ctattaattt
    64081 tttcaaagga gaagctcatg gcagaactca aacaagaatg gatcaacaca gctattgaag
    64141 cgttagataa agcctatgtt ccttattccc attttccggt aggcgcttgt ctcgtaacgg
    64201 aatcaggcaa gatttatcaa ggcatcaaca ttgagaatgc atcatttgga ttaacgaatt
    64261 gtgccgagcg cacagctttt ttcaaagcag tatctgaagg agaaaaaaca tttacgcatc
    64321 tagtcgtagc ggggcacacc cccgatccta tctctccttg tggtgcatgt cgacaagtaa
    64381 tggcagaatt ctgttcccct gatatgccgg ttacgctagt aggagacaat ggtgtgacga
    64441 aggcaacgac cgttcgtgca ctgctgcctt atgcatttac tgaaaaagat ctataactca
    64501 tgttcattca gtcagctgac tgttgaaaaa taaaacaatt attttaggag gctttacaga
    64561 aatgaaaaaa gcaaaattat ttggtttagg tgctgtcgca ctagcagcag ggttattttt
    64621 aggtgcatgc ggcaacaacg gctctactga ttcatccggt ggcaaaagct catcagacac
    64681 aacaactgca gcattgatca cagacacagg tggtgtagac gaccgttcat tcaaccagtc
    64741 agcttgggaa ggactagaaa aatgggggaa agatcaagga ctttcacgag gaaatgatgg
    64801 cttccaatat ttccaatctt caaatgaatc agattacatc ccaaatattg accaagcatt
    64861 gaatgctgga ttcaaaacga tttttggtat tggttataaa ctaaaaccag cgatccaaga
    64921 acaagcaaca aacaatacag gtacgaactt tgttattatc gatgatgtca tcgatggatt
    64981 agataacgtt gtatctgcaa ctttcaaaga caacgaagct tcttatcttg caggtgttgc
    65041 agcagcttat acgactgaaa caaacgtagt cggctttatc ggcggtgtaa aaggtgaagt
    65101 aatcgatcgt tttgatgctg gattcaaagc cggtgttgat gcaggtgcga aagaattagg
    65161 aaaagaaatc aaagtattga atcaatatgc tggtgacttc tctgctccag ataaaggacg
    65221 ttcaattgct caaggtatgt atgcacaaaa cgcagatatc atcttccatg cttcaggcgg
    65281 tacaggtaac ggtgtattcc aagaagcaaa atcattgaac gaatcaggcg acaaaaaagt
    65341 ttgggttatc ggtgttgacc gcgaccaatc agacgaaggc gaatacacat taaacggcga
    65401 aaagaaaaac ttcacattga catctacact gaaagcagtt ggaacagtag tagaagattt
    65461 ggcacaaaaa tcagcggacg gtaaattccc tggcggcgaa cacactgttt atggattgaa
    65521 agaagacggt gttggtctaa cagaaggaca actttctgac gaagcgaaaa aagcagttga
    65581 tgaagcaaaa gaaaaaatca tttcaggtga cgtaaaagtt ccagaaactc ctgaagaaaa
    65641 ctaaaaccat ttctatagcc ttgttagaca catgtttgac aaggctatgg tttgattaga
    65701 acaagaattg ttcatttatc caagctgcct tgtgacagtt tggataaggg aacaactccc
    65761 tgaagtaatg tttgtttcaa aagaagaacg ctcactaagc gtgaatctta gtcaatcgtt
    65821 gccctagata gaaatcgtta ggatgtgaaa aaaaggtggt tcaagaaaat attgtcatag
    65881 aaatgcagaa tattaccaaa gaatttggaa cattcaaggc aaatgacaac atcaatttac
    65941 aagtaaaggc aggagaaatt catgcgttgc ttggagaaaa cggtgctggc aaatctacat
    66001 tgatgaacgt gctttccgga ttattagagc cgacatcagg gaaaattttg atgcgtggga
    66061 aagaagtaaa gatcacaagt ccgaccaaag ccaaccaatt agggattggg atggtccatc
    66121 agcactttat gcttgttgat gcctttactg taacagaaaa catcgtgttg ggaagcgagc
    66181 ctagtcgcgc agggatgctt gaccataaaa aagcacgaaa agagatccaa aaagtttctg
    66241 aacagtatgg attgtcagtc aacccagatg cttacgttcg tgatatttca gttggaatgg
    66301 aacaacgggt agaaatttta aaaacacttt accgaggagc agatgtactg atttttgatg
    66361 agccgacagc tgtattgacc cctcaagaaa ttgatgaatt aatcgtgatc atgaaggaat
    66421 tagtcaaaga aggcaagtcg atcattttga ttacgcataa gttagatgaa atcaaagcag
    66481 tagctgaccg ctgtacagtt atccgccgtg gaaaaggaat cggtacagtc aacgttaaag
    66541 acgtaacctc acaacaatta gccgatatga tggttggaag aacggtttca ttcaaaacga
    66601 tgaaaaaaga agcgaagcct caagaagtcg ttttgtctat tgaaaatcta gtggtgaaag
    66661 aaaatcgtgg attagaagcc gtgaaaaact tgaacttaga ggttcgtgct ggcgaagtac
    66721 ttggtatcgc tggaatcgat ggaaacgggc agtcggagtt gatccaagct ttgactggtt
    66781 tgcgaaaggc agaaagcgga catatcaagc taaaagggga agacatcact aataaaaaac
    66841 cccgaaaaat cactgaacat ggtgtaggac acgtgccaga agaccgtcat aaatacgggt
    66901 tagtcctaga tatgacatta tctgaaaata tcgcactaca aacctatcat caaaaacctt
    66961 acagtaaaaa cggcatgctg aattattcag taatgaatga acatgccaga gaattgatcg
    67021 aagaatatga tgttcgaaca acgaatgaac ttgttcccgc aaaagcttta tcaggcggaa
    67081 atcagcaaaa agcaattatc gctcgtgaag tcgaccgaga tcctgatctg ttgattgttg
    67141 caaatccaac tcgtggactg gatgtaggtg cgatcgaatt tattcataaa cgtctgatcg
    67201 aacaaaggga caaatacaaa gcagtgttac tgatcagttt cgaattagaa gaaattttaa
    67261 atgtttcgga tcgtattgct gttatccatg aaggagaaat tgtcggtatc gttgatccga
    67321 aagaaacatc tgaaaatgaa ttaggtctat tgatggcggg ctatacctta gaagaagcac
    67381 gaaaagagtt agaaaagggg gctggcgcac atgaatgatc gcagcgaaaa attccgtaat
    67441 ttattagtgc ctgtactttc agttgtttta ggattgatct taggcgcgat attgatggct
    67501 gcttttggtt ttcagcccat ccaaggttat tcttctatgc tagatgcagc ccttggttcg
    67561 caaagaggga taggtgaaac gttaaggcaa gcaacgccgt tgatttttac agcattaggt
    67621 ttttctgtag ctaactcagc tgggtttttc aatattgggc tttctggaca agcactttgc
    67681 gggtggatcg tcagtgtctg ggtggcactt gcgtttccag atctgccaaa agttgttttg
    67741 cttccgttat gcgtgatctt aggtgcctta gctggtgcga tggcagcggc tgttccaggt
    67801 ttacttcgag cattttttgg aacaagtgaa gtgatcgtaa caatcatgat gaactatatc
    67861 ttattatacg taagcacatt tgctttacat gaagtgatgc ctgaaagcta tcgctcaagt
    67921 ttggactctt ccaatatgat cagtcaaaat gcctcactgc ggttaccatg gttaacacaa
    67981 gcttttggcg gctctcgcgt aaactcagga ttatttttag caatcatcgc gcttattttc
    68041 gtttggctgc tgatgaagaa aacgacttta ggatttgaaa tccgttctgt tggattgaat
    68101 ccttttgctt ctgagtatgc aggaatgagt agtaaacgta cgatcgtttt gtcgatggtc
    68161 atttctggcg ctttggcagg gcttggcggt gtggttgaag gattaggaac gtatcaaaac
    68221 ttctttgttc aaacgacttc gctatctatt ggatttgacg gaatggctgt ttccttgctt
    68281 ggttctggat cagcaatcgg tattctttta tcagcgatct tgttcagtat tttgaagatc
    68341 ggtggacttg gcatgcagac aggagcaggt gttccgtttg agatcgtcaa tgtatcgatt
    68401 gctttgatta tcttctttgt cgggatcaac ttcctgattc gtttcgtcat ggctaaagtc
    68461 ttccaaggga aaaaacaaga agagatcgtt gcgacaatag aatcgaaacc aacgaatcaa
    68521 aaccatgaag ggggcgaact ataatgtcga ctatcgaatt aattgccttg atccttactt
    68581 ctacactggt ttattcaaca ccgctaattc taacgtcact aggcggaact ttttctgaac
    68641 gaagcgggat cgtaaacgta gggctagagg gaatcatggt catgggtgct tttagttcca
    68701 tcgttttcaa cttgacgatg agtccatcac ttggttctat gacaccatgg atcgctattt
    68761 tagtgggtgg cgtcgtaggt atcctatttt ctctgattca cgcagtagca acagtcagtt
    68821 tgcgtgctga ccatattatc agcgggacag taattaatct gatggcacct gctttaggtg
    68881 ttttcttgat caaagcatgg tatggtaaag gtcaaactga taacatcacg cagaatctgg
    68941 ggtatttttc tttcccagga ttagcagata ttccggtcat tggaccaatt ttctttaaaa
    69001 atacattctt acctgcttat tgtgctattt tgattgctgt cctagcttgg gtgatcatct
    69061 tcaagacgaa attcggcttg cgcctccgtt cagttggtga aaatcctcaa gctgccgaca
    69121 cattaggtat caatgtatat gctatgcgtt attcaggcgt actgatttct ggcttgttag
    69181 gcggtatggg cggtgctgtt tttgctcagt cgatttccgg taacttttca gctggtacga
    69241 tcgttggaca agggttcatc tccatggctg ctatgatctt tggtaagtgg aatcctttag
    69301 gggcaatggg tgcctcattg ttctttggtt ttgctcaaag cttgagtatc attggcgcac
    69361 aattaccagt tatttcttcg attccgccag tattgctgca aattgctcca tatttgctga
    69421 caatcattgt gctggttgtt ttcttaggga aagcttctgg tccaaaagca aacgggaaga
    69481 actatatcaa atcaaaataa tcttttagcg aataaaaagg gtgggttacg ggaagtgttt
    69541 ttcgtctcgc cctttttgtt ttaaaggcaa taaaaataaa aagaaagaag gaataatcat
    69601 gtttaaacgc gtacatctga tcgtcatgga ttcagtagga attggagaag caccagatgc
    69661 cgataagttc ggtgatgttg gttctgatac attaggacac attgcgaaag aagcaggatt
    69721 gaacgttcca catttagagc agcttggttt aggaacgatc cgtcctcttg aaggtgtgga
    69781 atcagtgaaa gatcatcaag gctatgcgac gaaattagaa gaagtttctg tagggaaaga
    69841 tacgatgaca ggccactggg aaatcatggg attgaatatc aaaactccat ttcgtgtgtt
    69901 tcctaatgga ttccctgatg atctgatcaa acaaatcgaa gaattttctg gccgtaaagt
    69961 cgtatgtaac aaaccttata gcggaacagc tgttattgac gattacggtg aacaccaaat
    70021 gaaaacaggc gatttaattg tatatacttc tgccgatcct gtccttcaga tcgcggcaca
    70081 tgaagaaatc attcctttag aagaattata tcggatttgc caattcgttc gtgatataac
    70141 aaaagatgat ccgtatatga ttggccgaat cattgcacgt ccgtacgtgg gtgaaccagg
    70201 agacttcaca cgaacaagca atcgccatga ttatgcgttg aatccattcg gacacacagt
    70261 ccttgattct ttgaaagaag caggaaagga cgtgattgct gttggaaaga tcaacgatat
    70321 tttcaacgga caaggaatca ctgaatctgt ccgcacgaaa agcaatatgg atggcgttga
    70381 ccagctgctg aaagttatga aaaacgattt ttcaggaatc agctttacca atttagtaga
    70441 ttttgatgca ttatacggtc atcgtcggga tgtagtcggc tatgcgcatg cgattgaaga
    70501 atttgactta cgaattccag aattgcttga agcgatggaa gaagacgact tgctaatgat
    70561 tacagccgat cacggaaacg atccgacgtt tactggtacc gatcatacgc gagaatatgt
    70621 accgttattg gtttacagta aaaaaatgaa agaacaaggg gcattgcctc aaggatatta
    70681 ttcagatatt tcagcaacga ttgctgagaa ctttagtgtt tcttcgactg aaaatggcga
    70741 aagcttcttg aaattattaa aataatcttt ttaaatagga gaaagcaaat gaccaagtta
    70801 agcgagatgt tacaagaaac gacccagttt atccttgata aaggagtaaa agaaattgat
    70861 tttggtttga ttctaggttc aggattaggt gaattagcag atgaaatcga agatgctgta
    70921 atgatttctt atgatgagat cccgttcttt cctgtttcga cagtagttgg gcatgcagga
    70981 aagctagttt atgggtctct ggcaggcaag aaagtgttag ccatgcaagg gagattccac
    71041 tactatgaag gtcattccat gcaaacagtg acctatccag tacgtgtgat ggccggacta
    71101 ggggcccatt cagtcattgt gactaatgca tgtggtggtg tcaacgaatc atttgtacca
    71161 ggagatttga tgctgattac cgatcatatc aattttactg gtgaaaatcc tttgatcggt
    71221 ccgaatgaag atgcaatggg ccctagattt ccagatatga gtcaagctta tacgcctgac
    71281 tatcaagcaa cagcaaaagc agcagcagaa aaactaggtt tggatcttaa agaaggcgta
    71341 tatatgggct attctggacc aacttatgaa acgcctgcgg agatccgaat gtctcggaca
    71401 ataggagccg atgcagtagg gatgtcgact gttccagaag tcatcgtagc agctcacagc
    71461 ggattaaaag tgttaggaat ctcttgtatt accaacttag cagcaggtat gcaagcgaat
    71521 ctgagccatg aagaagttgt agaaacaact cagagagtca aacaatcttt taaagcattg
    71581 gttaaagaaa cattgacatt attgtaatag gaggaacaat aaaatgagtg tgcatattga
    71641 agcgaaagaa ggcgagatcg ccgataagat tttacttccg ggagatcctt tgcgagctaa
    71701 atatattgcg gagacttttt tggaagatcc agtttgttac aatcaggtac gcggtatgtt
    71761 aggctttacc ggcaaatata aaggagaacg tatttctgtt caaggaacag gaatgggaat
    71821 gccatcagcc accatctatg cccatgaatt gatccagtcg tatggtgtaa agaaattgat
    71881 tcgagtaggt acatgcggtg cattgtcaaa agacgtccat gttcgtgatt tagtactagc
    71941 acaaggtgca gcgaccagtt cttcgatgat cgaaaaaaat ttccaagcct ttcattttcc
    72001 tccaatcagt gatttcaatc tattactgaa agcatatgag atagctaaag aaaagggcta
    72061 tacggttcat gtaggaaatg ttctttcgga agattctttc tacaaagatg atttgacaga
    72121 gacgttccaa ttagctgaac ttggcgtttt aggagtggaa atggaagcag cggctcttta
    72181 ttacttaggc gcgaaatacc atgtgcagac attaagtttg atgaccgtta gcgaccattt
    72241 gatcaccggt gaagaaacaa cagctgcaga acgtcaatct acgttcaatg aaatgatcga
    72301 agtaggattg gaaacagcga tagcagaata agaaggaaat gaaaaagctg ccctgcatta
    72361 agtaataaga acatccaagc aaaaatagct cctcatgttt ttgacttggc tggacttatt
    72421 attgcaaatg taagcttttg aacaccgttt accaagaggt tgtgaatctg ccgtttaact
    72481 ctgagtcaca aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt
    72541 gtgaccgaga agttggcagg agaaaaccgt ttatacgaaa aaagggactg tgacaaatcg
    72601 tgtcacagtc ccttttttat agtgtttttt ataggtagta ttttttagca actgttaagt
    72661 catcatttaa ttcataaaca agtggttgac ctgttggaat ttcaagatcc atgatgtctt
    72721 cgtcagagat accttcgata tgtttagcta gggcgcgtaa tgagttacca tgtgcagcta
    72781 ctaaaacagt tttgttgtct agtaatgctg gagcaatttc atcttgccag aatggcaaag
    72841 cgcgttctaa tgtcactttc aagttttctc caccaggaat atcgcgtttg tctaacattg
    72901 cgtaacgacg gtcatttgct gcagaacctt cgtcagtttc ttccattaat ggaggaagag
    72961 tatcgtaaga acgacgccag atgtgcactt gttcgtcgcc gtatttttca gctgtttctt
    73021 ttttgttcaa accttgtaat ttaccatagt gacgttcgtt taagcgccat gatttgattt
    73081 gtggtaccca aagttgatct gaatattcta agatcaagtt acatgttttg attgcacgag
    73141 ttaaaacaga agtgtaagca acatcaaatt cgattccagc ttctttgatt ttgcgtccgc
    73201 cttcttttgc ttcttcgatt ccttctggtg ctagatttac atcagcccaa ccagtaaatt
    73261 ggttcaatgc attccattca ctaagaccgt gacgagaaaa aactaatttt ggcatgttta
    73321 gctctccttt taagataggt ttatatttct gaaattttca gttctgtttt attgtacaat
    73381 tttttggaag agatttccat cgaaaaatta gataagattc tatgagaaaa caagtagaga
    73441 atataaaaaa acgaacagtg cccacgcaac tgttcgtttt taggtattag atgcataatt
    73501 gctcgatttc tttttcaggt aaattgatat atttataaca tgttccaact gaaacaccgc
    73561 attttgaaga aataaattgg atcgtttctt ttttttcata atagagacgt cggatttttt
    73621 tgatcgtttt tgcatcgatt tttggtcttc cgccgatttt tcctttttta cgagcttcat
    73681 ctaatccaac taatgtacgt tctttgatca atgcgcactc catgtttgct aggttttcca
    73741 ttaattggaa atagattttt cccatttcgc gcgtatcgat tccttcatca agactaatca
    73801 agtgcagccc gcgatctttg aataattgcg tgagttctgt taattgccgc gtagatttgc
    73861 ctaatcgatg aagttggcaa atgactagtg catcgccttg ttgcatgtca gcgatcactg
    73921 actccaaatg aacagcagta gaatcatttt gttccacttc atatgtctca tggaaaattt
    73981 cttgacaacc aaagtcagat aattgatcaa cctgagttgt taaatcaacg tcatttatgg
    74041 ttgtgcgtgc ataaccaata attttcatgg tgattccttt ctagcagtga ttttctaact
    74101 ggatataaca gtaaaaatgt agcataaagt acgtcaaaaa gatagttttt tcgcaaattt
    74161 tccgctgtct ttttttattg gatgatcaag cagtttttct ttatttacga cggattgcgt
    74221 caagaatctc aacgcctttt tctgagccga caactacttt agaaaccatg tttaggaaaa
    74281 gcccatgttc tactacccca acaagttcat ctaaataggt agctaagctt tctggctgtt
    74341 cgatgacttc tagatgcaga tcaatgatgt aatgaccacc atctgtggtt aagacttctc
    74401 cgttttcgtc tgtacgcaag acgggttgga atcttttttc ttcaaaaata tgaagtagct
    74461 gttgggagcc ataagggatc acttcaactg ggagtgggaa cttcccaagt ttatcgacta
    74521 atttagaaga atcaacgatc caaatcgttt cttttgagta cgtagcgact attttctcga
    74581 aaagcaaagc agcgccgccg cctttgatcc cttgaaaatc agcactgatt tcatcagcac
    74641 cgtctattgt caaatcgact acggggactt catcaatact ctttaacggt attcctaatt
    74701 tttccgcttg ttcttttgtc gcatttgaag tagtaactcc tgtgatacgt aaaccttctt
    74761 cacgcatacg gcgaccaatc tcttctacca tataataggc ggttgaaccg gtcccgagac
    74821 ctacgatcat cccgtccttt acgaatttcg cggcttcaat gccagctaat tgttttaaat
    74881 tcatctttgc gtccaccttt ttttagtttg agcatcgtgc tttcctcact attttagcgt
    74941 gatttacggt aaaaagatac cattcttttt tattccaata aagctttttc aaaaaaaatt
    75001 gcatctcttg ttgacatgtt ttttcattcg tgatattcta acaaaggtgc tttatctaca
    75061 ggtttctgtg aagaaacgtg ctgactgttt atcaaagcat atttgataag gatgcagaca
    75121 ttgcatttat tttttatcac aaaaaaagaa ccacctggat gtgtggaact agacgcaaat
    75181 cgtgggaagg aggaaacaag gaatgcctac aattaaccaa ttagtacgta aacctcgtaa
    75241 atcaaaggtg gaaaaatcta attcacctgc tttgaacaaa ggatataaca gctttaagaa
    75301 aactcaaact aacgtaaact cacctcaaaa acgtggggta gcaactcgtg tgggtacaat
    75361 gacacctaaa aaaccgaact cagctttacg taaatatgca cgtgttcgtt tgtcaaactt
    75421 aattgaagtt actgcttata tcccagggat tggtcataac ttacaagaac acagtgttgt
    75481 attattacgc ggtggtcgtg taaaagactt accaggggta cgttaccata tcgttcgcgg
    75541 tgctttagat actgctggtg ttaacgaccg taaacaaagc cgttctaaat acggaactaa
    75601 aaaacctaaa gcttaatttt aattattaaa caaatgaaac ttgcttctac tatatagaag
    75661 taattactat gaacaatatt tcggaaggag gagttacgga tgccacgtaa aggtcctgtt
    75721 gcaaaacgtg atgttttacc agatcctatt tataactcaa aattagtaac tcgcttgatc
    75781 aaccgtgtaa tggttgacgg aaaacgcggg attgcagcta acattatcta taatgccttt
    75841 gacttgatca aagaatctac aggtaacgat ccattagaag tgtttgaaca agcaatgaaa
    75901 aacgttcaac ctgttcttga agttaaagca cgtcgtgttg ggggttcaaa ctaccaagta
    75961 ccagttgaag ttcgccctga acgccgcgtt actctaggtc ttcgttgggt agtaaactac
    76021 gctcgcttgc gcggtgaaca cacaatggaa gaacgcttag caaaagaaat catggatgct
    76081 gcaaacaaca caggtgcttc tgttaaaaaa cgtgaagaca ctcataaaat ggctgatgcc
    76141 aaccgtgcgt ttgctcacta tcgctggtaa gattctctct gtttggtcgg cttttataag
    76201 tcgacctcag atagtgtcct tatttttagc aactagatag taaagagagg agaaacaagg
    76261 agaatgacca gagaattttc tttacaaaac actcgtaata tcggtatcat ggcccacatc
    76321 gatgctggta aaacgactgc tactgaacgt atcttgtact atactggtaa aatccataaa
    76381 attggtgaaa ctcatgaagg agcttcacaa atggactgga tggaacaaga acaagaacgt
    76441 ggtattacga tcacttctgc tgctactaca gcacaatgga agggtcaccg aatcaacatc
    76501 atcgacacac caggacacgt agacttcaca gttgaagttg aacgttcatt gcgtgtactt
    76561 gatggtgctg taactgttct tgatgcacaa tcaggtgttg aacctcaaac tgaaacagtt
    76621 tggcgtcaag caacaactta tggtgtacct cgtatcgtat ttgcaaacaa aatggacaag
    76681 atcggtgctg acttcttata ttcagtatca actttacatg accgtttgca agcaaacgct
    76741 cacccaatcc aattaccaat tggtgcggaa gacgacttca caggaattat cgaccttgta
    76801 acaatgaagg ctgaaatgta tacaaacgat cttggaacag agatcgaaga aacagaaatt
    76861 cctgaagaat accgcgaatt agctgaagaa tggcgcgaaa aattggttga agctgtagct
    76921 gaaactgatg aagaacttac tttgaaatac ttagaaggtg aagaaatcac tgaagctgag
    76981 ttaaaagaag gtatccgccg cgcaacagtt aacgtagaat tctacccagt actatgtggt
    77041 tcagcattta agaacaaagg ggttcaatta ctattggacg ctgttcttga ttacttgcca
    77101 tcaccgttag atatcccagc tatcaaaggg attgacccta aaactgatga agaagtagaa
    77161 cgtcctgcag acgacagcgc tcctttctca gctttagcat ttaaagttat gactgaccca
    77221 ttcgtaggtc gtttaacttt cttccgtgtg tactctggtg ttttacaatc aggttcatac
    77281 gtacaaaacg caacaaaagg caaacgtgaa cgtgttggtc gtatcctaca gatgcatgcc
    77341 aactctcgtt cagaaatctc tgaagtttac gctggagata tcgctgctgc tgttggtttg
    77401 aaagacacaa ctactgggga cacactatgt gatgaaaaga gtctagttat tcttgaatcc
    77461 atggaattcc cagaaccagt tatccaagtc gctattgaac ctaaatcaaa agctgaccaa
    77521 gataaaatgg gtgtggcttt acaaaaacta tctgaagaag atcctacttt ccgtgctgaa
    77581 acaaacgtgg aaacaggcga aacaatcatc gctggtatgg gtgaacttca cttagatatc
    77641 atcgttgacc gtatgcgtcg tgaattcaaa gttgaagcta acgtaggtgc gcctcaagta
    77701 tcttaccgtg aaactttccg tgctggcact caagccgaag gtaagtttgt acgtcagtct
    77761 ggtggtaaag gtcaatacgg tcacgtatgg attgaattta caccaaatga agaaggtgct
    77821 ggtttcgaat ttgaaaatgc gatcgtcggt ggggttgttc ctcgtgaata cattccagct
    77881 gttgaaactg gtttgaaaga tgctatggaa aatggtgtct tagctggcta tccattagta
    77941 gatatcaaag ctaaacttta cgatggttca taccatgatg tcgattcaaa tgaaactgcc
    78001 ttccgtgttg ctgcttctat ggcattacgt gcagcagcta agaaagctaa tccagtaatc
    78061 cttgaaccaa tcatggctgt agaagttgtt atccctgaag attatttagg tgatgttatg
    78121 ggtcacgtaa ctgctcgtcg tggacgtgtt gaaggtatgg aagctcgcgc tggcggccaa
    78181 caagttgttc gtgcgatggt tccacttgct gaaatgtttg gttatgctac aactcttcgt
    78241 tctgctactc aaggtcgtgg tacatttaca atgacatttg accactacga agatgtacca
    78301 aaatcagtac aagaagaaat cattaagaaa aacggcggaa aagctgaata atttcttgaa
    78361 accggatagt cctgcaaaga gattatccaa agagactata tttttaatat agtttcgtgt
    78421 aaaataatta tcgatgatat gggcatgtgg tgtaccttgt gtacatcata tacctatcag
    78481 acaaataaaa aatatattgt atttttagga ggacatttta aaatggcaaa agaaaaattt
    78541 gaccgttcta aaccacacgt taacattggt actatcggac acgttgacca tggtaaaact
    78601 acattaacag ctgcaatcac aactgtacta tctaagaaaa acggcggcca agctatggct
    78661 tacgatcaaa tcgatggtgc tccagaagaa cgcgaacgcg gaatcacaat ctcaacagct
    78721 cacgttgagt atgaaacaga tacacgccac tacgctcacg ttgactgccc aggacacgcg
    78781 gattacgtta aaaacatgat cactggtgct gctcaaatgg acggagctat cttggtagtt
    78841 tctgctgctg acggcccaat gcctcaaact cgtgaacaca tcctattgtc tcgtcaagtt
    78901 ggtgttcctt acatcgttgt attcttgaac aaagtagaca tggttgatga cgaagaatta
    78961 ctagaattag ttgaaatgga agttcgtgac ctattaacag aatacgaatt ccctggtgac
    79021 gatgttcctg tagttgctgg atcagctttg aaagctctag aaggcgacgc ttcatacgaa
    79081 gaaaaaattc ttgaattaat ggctgcagtt gacgaataca tcccaactcc agaacgtgac
    79141 aacgacaaac cattcatgat gccagttgaa gacgtgttct caattactgg acgtggtact
    79201 gttgctacag gtcgtgttga acgtggacaa gttcgcgttg gtgacgaagt tgaagttgtt
    79261 ggtattgctg aagaaacttc aaaaacaaca gttactggtg ttgaaatgtt ccgtaaattg
    79321 ttagactacg ctgaagctgg agacaacatt ggtgctttac tacgtggtgt tgcacgtgaa
    79381 gacatccaac gtggacaagt tttagctaaa ccaggtacaa tcacacctca tacaaaattc
    79441 tctgcagaag tatacgtgtt gacaaaagaa gaaggtggac gtcatactcc attcttcact
    79501 aactaccgtc cacaattcta cttccgtaca actgacgtaa caggtgttgt tgaattacca
    79561 gaaggaactg aaatggttat gcctggcgac aacgtaacaa tggaagttga attaatccac
    79621 ccaatcgcta tcgaaaacgg tactaaattc tcaatccgtg aaggcggacg tacagttggt
    79681 gccggtgtcg ttacagaaat caaagcttaa ttcacttaag ttttaaagta aagcaacatt
    79741 tattcggacg taatcgctcg gaacgaaggt cttaggatct ttgttccgag cttttttatt
    79801 tattgcagga tcaggaaaaa agacaggagt attataaata gaagaaacac gaaataaaag
    79861 agtcagtatt ttctaaatga aactttatga aaacaaaatg ttttttacaa gaaaaagcct
    79921 tgctttgtga gtttatatat attataatga caaaggtgcg tttactttag tgaattgcac
    79981 agacgtaatc agaatgattt cgtcggctaa gaaacgagag gttgcgacac gcccggacgc
    80041 tttgccacga acgagcgtga cggaaatttt cgtggagcta tgtctacttt tcaaaatagg
    80101 cgaaggaggg aacaagatgg caaaacaaaa aattcgtatc cgtttaaaag cgtatgaaca
    80161 ccgtatttta gatcaatcag cggataaaat cgtggaaact gcaaaaagaa ctggagctag
    80221 cgtgtctggt ccaattccat taccaactga acgcagtctt tacacaatca tccgtgcgac
    80281 tcataaatac aaagattcac gcgaacaatt cgaaatgcgt acacacaaac gtcttatcga
    80341 cattgtgaac ccaacaccaa aaactgttga tgctttgatg aagctagact taccatctgg
    80401 tgtaaacatc gaaatcaaat tataaaacta aaacaaaaat aatggaggtg tactcatgac
    80461 caaaggaatc ttagggaaaa aagtgggaat gacacaaatc ttcactgaat ctggtgaatt
    80521 aattccagta actgttgtag aagctactcc aaacgtagta ctacaagtga aaacaatgga
    80581 aacggatggc tacgaagcta ttcaagttgg ttaccaagat atgcgtgaag ttttatcaaa
    80641 caaacctgcg aaaggtcatg ttgcaaaagc aaacacggct cctaagcgct tcattcgtga
    80701 attcaaaaat gttgagctag gagaatatga agtaggaaaa gaaattacag tagatgtttt
    80761 ccaagcagga gacattattg atgtaacagg tactacgaaa ggtaaaggat tccaaggggt
    80821 tatcaaacgc cacggacaaa gccgcggacc aatggctcac ggttctcgtt accatcgtcg
    80881 tcctgggtca atgggtccag ttgcacctaa ccgtgtattt aaaaacaaac gtcttgctgg
    80941 ccgtatgggt ggcaaccgcg taactatcca aaacttggaa gttgtacgtg tcgatgcaga
    81001 aagaaatgtt atcttgatca aaggtaatgt tcctggagct aaaaaatctt taattactat
    81061 caaatcagct gtgaaagcta aataattgta ggaagaaagg aggaactaag gaatgccgaa
    81121 tgtagcatta ttcaaacaag atggaagcca aaacggtgaa atcacactta acgaagaaat
    81181 cttcggaatt gaacctaatg aaagtgttgt ttacgatgca atcgtaatgc aacgtgcgtc
    81241 attaagacaa ggaacacacg cggttaaaaa ccgtagcgct gttcgcggcg gcggacgtaa
    81301 accatggcgt caaaaaggaa caggtcgtgc acgtcaagga tctatccgct caccacaatg
    81361 gcgtggaggt ggcgtagtct ttggaccaac accacgttct tacagctaca aacttcctaa
    81421 aaaagttcgt cgtttagcaa tgaaatctgt attgtcagaa aaagttgctg aaaacaactt
    81481 ggtagcaatc gaaggcttga acttcgacgc accaaaaaca aaagaattca aacaagttct
    81541 tgctaactta tctatcgact ctaaagtatt ggttgttcta gaaaaaggta acgactttgc
    81601 tgctttatca gcacgtaact taccaaacgt ttctgtagta acttctgata atgtgagtgt
    81661 gctagacgtt gtatcaaata caaaagtgtt agcaacacaa actgctctta ctcaaattga
    81721 ggaggtgctt gcataatgaa cttactagac gtaatcaaac gcccagtgat cactgaaaaa
    81781 tcaatgcttg ccatggatga caagaaatac acttttgaag tggacactcg cgcaaacaaa
    81841 actttagtaa aacaagcggt agaagctgct tttgacgtta aagtcaaaaa cgtgaacatc
    81901 gtgaacgtac gtgcgaaatt caaacgtatg ggcaaatatg caggatatac aaaaaaacgt
    81961 cgcaaagcga tcgttacatt gactgaagat tcaaaagaaa tccaattatt cgaagctgct
    82021 gaataagcac taatagataa ctcattaaat taggagggaa aaacgtggcg attaaaaagt
    82081 acaaacctac ctcaaatggt cgtcgtaaca tgacaggttc tgattttgct gaaatcacaa
    82141 catcaacacc tgaaaaatca ttgttacaac cattgaaaaa ccgtgctgga cgtaacaaca
    82201 acggccgcat cactgtacgt catcaaggtg gcggacacaa acgccaatat cgcgtgatcg
    82261 acttcaaacg taacaaagat aacgttgttg caactgttca aacgatcgaa tatgatccaa
    82321 accgttcagc taacatcgcg ttagtacact atgaagatgg agtgaaagct tacatcttag
    82381 cacctaaagg actagaagtg ggtatgaaac ttgtttcagg tccagaagca gatatcaaag
    82441 tcggaaatgc tcttccatta gaaaacattc cagttggtac tgttatccac aacattgaaa
    82501 tgaaacctgg taaaggtgga caattgatcc gttctgctgg tacaagtgct caagtacttg
    82561 gtaaagaagg caaatacgta ttgatccgct tgaactctgg tgaagttcgt atgatcttag
    82621 ctgcatgccg tgctacaatc ggttctgtag gtaacgaaca acacgaatta atcaacatcg
    82681 gtaaagctgg acgctctcgt tggatgcgta aacgcccaac tgtacgtggt agcgtaatga
    82741 accctaatga tcacccacac ggtggtggtg aaggtaaagc tcctatcgga cgtaaagcac
    82801 cagtttctcc ttggggtcaa ccagctcttg gttacaaaac tcgtaacaaa aaagctaaat
    82861 cagacaaact tattgttcgt cgtcgcaaaa ataaataatt actttgacca tgtgtcgcac
    82921 attttgagag gaggttcacc atgggtcgta gtttaaagaa aggacctttt gtcgatgagc
    82981 atttaatgaa aaaagttgaa gctcaagcag gggttgaaaa gaaaaaagtt atcaaaactt
    83041 ggtcacgccg ttcaacagtt ttcccaagtt ttgttggata taccatcgca gtttatgatg
    83101 gacgtaaaca tgtaccagtt tacatccaag aagacatggt aggacacaaa ttaggtgaat
    83161 ttgcacctac aagaacttat cgtggacatg ctgctgacga taagaaaacg aaacgctaat
    83221 ttgaggggag gataaatcca aatggcagaa caaatcacat cagctaaagc aactgcaaaa
    83281 acagttcgca cttcacctcg aaaagcccgt ttagtaattg accttatcag aggtaaaagt
    83341 gtcgcagatg caatttcaat cttgaaattc acaccaaaca aatctgctgg aatcattgaa
    83401 aaagtattga tgtcagcagt tgcgaatgca gaaaataact ttgacttaga tgttgaaaac
    83461 ttggtagtaa gcgaagcttt tgttaacgaa ggacctacaa tgaaacgttt ccgtcctcgt
    83521 gcaaaaggat cagcttctcc aatcaacaaa cgtacaagtc atatcacggt agtcgtatca
    83581 gaaaaataag gagggataat cagtgggtca aaaagtacat ccaattggaa tgcgtgtagg
    83641 catcatccgc gactgggatg ctaaatggta tgctgaaaaa gagtatgctg agttcttaca
    83701 cgaagattta agaatccgta aatttatttc aactaaactt gctgatgctg ctgtatcaac
    83761 agttgaaatc gaacgcgcaa caggtcgcgt aaacatttca attcacacag ctaaaccagg
    83821 tatggttatt ggtaaaggcg gttctgaagt cgaaagccta agaaaagaat taaacaaatt
    83881 aactggtaaa agaatccata tcaatatcgt ggaaatcaaa aaaccagatc tagatgcaaa
    83941 attagtaggt gaaggaatcg cacgtcaatt agaaaaccgt gttgctttcc gtcgcgctca
    84001 aaaacaagcc atccaacgct caatgcgtgc tggcgctcaa gggatcaaaa ctcaagtatc
    84061 tggtcgtcta aacggagcag atatcgctcg ttcagaagga tactcagaag gaactgttcc
    84121 tcttcatact ttacgtgcgg acattgatta cgcatgggaa gaagcagata caacatacgg
    84181 aaaactagga gttaaagtgt ggatctatcg tggagaaatt cttccaacta aaaaaaacac
    84241 tgagaaagga gggaaataat catgttagta cctaaacgtg taaaacaccg tcgtgaattc
    84301 cgcggaaaaa tgcgcgggga agctaaaggc ggaaaagaag tagcattcgg tgaatacggt
    84361 ttgcaagctg ttgattcaca ttggatcaca aaccgccaaa tcgaagctgc tcgtatcgca
    84421 atgactcgtt acatgaaacg tggtgggaaa gtatggatta aaattttccc tcacaaatct
    84481 tatactgcca aagcaattgg ggtacgtatg ggttctggta aaggggcacc tgaaggatgg
    84541 gttgcaccag taaaacgtgg taaaatcatg tttgaaatcg caggcgttcc tgaagaagta
    84601 gctcgtgaag cgttacgtct agcttctcac aaattaccaa tgaaaactaa gatcgtaaaa
    84661 cgtgaggaaa tgggtggtga atcgaatgaa ggttaaagaa atcagagaat taaccactgc
    84721 cgaaatgctt gatcaagaaa aacaattaaa agaagaattg tttaatctta gattccaatt
    84781 agcaacaggt caattagaaa acactgcacg tattaaagaa gtacgtaaat cgattgcacg
    84841 catcaaaact gtgttgcgtg aacaagctaa gtaattatgg aaggaggcca ttaagcgtat
    84901 gactgaagga agaaatcaac gcaaagttta tcaaggtcgc gtggtttcag acaaaatgga
    84961 taaaacaatt accgttgctg tcgaaacaaa gaaaaaccac cctatctatg gcaaacgcat
    85021 gaactattct aagaaataca aagtgcatga tgaaaacaac acagcaaaag ttggcgacat
    85081 cgtgagaatt atggaaactc gtccattatc agctacaaaa cgtttccgtt tgttggaagt
    85141 agtcgaagaa gcagttatta tctaataaca cgagattttc ctatatagat tgaatgcaaa
    85201 atctgaaagg aggatacaca acgtgatcca agcagaaagt cgtttaaaag tcgctgataa
    85261 ctcaggcgca cgtgaaattt tgacgatcaa agtccttggt ggatctggtc gtaaaactgc
    85321 taacatcggt gatgtgatcg ttgctacggt taaacaagca acgccaggtg gggttgtcaa
    85381 aaaaggtgac gtcgttaaag ccgttatcgt tcgaacaaaa tcaggagctc gtcgtacaga
    85441 cggttcatat attaaatttg atgaaaatgc tgcagtgatt attcgtgatg ataaaagccc
    85501 tcgtggaaca cgtatctttg ggccagttgc acgtgaacta cgtgaaaaca acttcatgaa
    85561 gatcgtttca ctagcaccag aagttttata atcttgagac acttatcaaa ggaggtgcga
    85621 aacagttatg tttgttaaaa aaggcgataa agttaaagtt atcactggaa aagacaaaaa
    85681 caaagaaggc gttgtactag cagcgttccc gaaaaaagat aaagttattg tcgaaggtgt
    85741 aaatgtcgtt aaaaaacacc aaaaaccttc tcaagctgct cctcaaggtg gaattgttga
    85801 gatggaagcg ccaattcatg tttctaacgt gatggtggtt gactctactg gtgtagctgg
    85861 ccgagttggc tacaaagaag tagacggcaa aaaagtccgt gtttctaaaa aaaccggtga
    85921 agttttagat aaataaaagt ttaaggaagg agggtcttac tgaatgaacc gcctaaaaga
    85981 aaaatatctt aaagaagtaa ctccatcatt gatggaaaaa tttaactata gctctgttat
    86041 gcaaacacct aaagttgaaa aaatcgtcat caacatgggt gttggtgatg cggtatcaaa
    86101 cgctaaaaac ttagataaag cagttgaaga attaactttg atcactggtc aaaaaccaat
    86161 gattacaaaa gctaaaaaat caatcgctgg attccgttta cgtgaaggaa tgccaattgg
    86221 tgcaaaagtt acattgcgcg gagaaagaat gtacgaattc ttagacaaat tggtttctgt
    86281 ttcactacca cgtgtacgtg acttccatgg tgtaagtaaa aaagcctttg acggtcgtgg
    86341 aaactatact ttaggtatta aagaacaatt gatcttccca gaagtagatt atgatttagt
    86401 agataaagta cgtggtatgg acatcgttat tgtaacaaca gcaaactctg atgaagaatc
    86461 tcgtgagttg ttgacacaat taggcatgcc attccaaaaa taattaaagg aggcgaacta
    86521 cgtggctaaa aaatcaatga ttgctaaaaa caaacgtcct gcaaaatttt caacacaagc
    86581 ttatactcgt tgcgaacgtt gcggacgtcc acattcagtt tatcgtaagt tccatctttg
    86641 ccgtatttgc ttccgcgaac ttgcctataa aggtcaaatt cccggcgtga agaaagctag
    86701 ctggtaaaaa gtaaattcgc ataagaagga ggtaaaagat caatggtcat gacagatcca
    86761 attgcagatt ttctaactcg tatccgtaac gcaaacatgg ttaaacatga gtctttagaa
    86821 gtacctgcat caaaaattaa acgtgacatc gctgaaatct tgaaacgtga aggtttcgtt
    86881 cgtgatgttg aatacatcga agatgacaaa caaggcgtga tccgtgtttt cctaaaattt
    86941 ggtaaaaacg gcgaacgtgt tatcacaaac ttaaaacgta tctctaaacc aggtttgcgt
    87001 gcttatgtca aagctgacga agtaccaaaa gtattgaacg gtttaggtat cgcaatcatt
    87061 tcgacttctg aaggtgttgt cactgataaa gaagctcgtg aaaaaaatat cggcggcgaa
    87121 gttatcgctt acgtatggta atcaagtaga atctacaagg aggtgtcttt aagtgagccg
    87181 tatcggaaat aaagtcgttg ttatccctga aggagtaaca gttactcaag acggaaacaa
    87241 cgttacagtt aaaggaccaa aaggtgaatt gactcgtact ttttcagcag atatcaaaat
    87301 gaatatcgaa ggaaacgaag taactttcac tcgtccaaac gacagtaaag aaatgaaaac
    87361 aattcatgga acaactcgtg cgaacttcaa caacatggtt gtaggtgtca gcgaaggttt
    87421 ccaaaaagcg ttagaactaa tcggggttgg gtaccgtgca caattacaag gatcaaaact
    87481 tgtattgaac gttggttact cacatccagt agaaatcaca ccaccagctg gagtaactgt
    87541 tgaagtacca gctaacacac aagtaattgt taaaggtgcg aacaaagaag aagttggcga
    87601 attagctgca aacattcgtg gaactcgtcc tccagaacct tataaaggta aaggtattcg
    87661 ttatgttggc gaatatgtac gccgtaaaga aggtaaaact ggtaaataat cgtttagaaa
    87721 aattgcatag aagccagcag caggaaaaag gcatttggct gaagtttacc ttcaaaaaaa
    87781 tacattttta cttgtgccgc agcttctatt tttcgaaacg tatttactgc ttgaaatagt
    87841 agtacaataa aaataaagag gtgacaattg tgattacaaa accagataag aacaaaacac
    87901 gtcaaaagag acatcgtcgt gtacgtaaca atatctctgg tactgctgag cgcccacgct
    87961 tgaacgtttt tcgttctaac aaaaacatct acgctcaaat tattgatgac gtagcgggtg
    88021 taacgctagc aagtgcctct accttggata aagaaatttc aggtggaaca aaaacagaag
    88081 cagcacaagc tgtcggtaaa ctagttgctg aacgtgcagc tgaaaaaggc attaaagtag
    88141 tagtttttga ccgtggtgga tacctttacc atggccgtgt gcaagcttta gctgaagctg
    88201 ctcgcgaaaa tggactagaa ttttagaaga aggaggaata ccattcatgg tttatatcga
    88261 tccaaaacat ttggaattag aagaccgcgt tgttgcaatt aaccgtgtaa caaaagttgt
    88321 taaaggtgga cgtcgtctac gttttgctgc tttagttgtt gtcggtgaca aaaacggaca
    88381 cgtaggattt ggtacaggta aagctcaaga agttcctgaa gcaatccgta aagcaattga
    88441 agatgcgaag aaaaacttag ttgaagtacc tatggttggt tcaacaatcc cacacgaagt
    88501 aatcggtgct tttagtggtg gtagaatcct aatgaaacct gctgtagaag gttctggggt
    88561 tgctgctggt ggaccagttc gtgccgtatt agaattagct ggggtagcag atatcacttc
    88621 taaatcttta ggctcaaata caccaatcaa cgttgttcgc gcaacagttg aaggtttgaa
    88681 acaattgaaa cgtgcagaag aagttgcaga acttcgcggc aaatcagttg aagaattaat
    88741 cggataagga ggacaacaat aatggctgaa ttaaaagtta ctttaaaacg cagtattatc
    88801 ggacgtcctc aaaaccaacg cgatacagtt aaagcgttag gtcttacaaa gattaacagt
    88861 tcagtggtta agccttctaa tgaagcaatt aaaggcatga tcaatactgt ttctcaccta
    88921 gtggacgttg aagaaattta atttagaaaa atcataacta gaacattaag gaggtgccaa
    88981 acctatgaaa cttcatgaat taaaacctgc tgaaggttca cgccaagtac gtaaccgtgt
    89041 tggtcgtggt acttcatctg gtaacggaaa aactgccgga cgcggacaaa aaggacaaaa
    89101 agctcgttca ggcggcggtg tgcgtttagg atttgaaggg ggacaaacac cattgttccg
    89161 tcgtttacca aaacgaggat tcacaaacgt gaaccgtaaa gactatgcag tcattaatct
    89221 tgatgtattg aaccgctttg aagacggagc tgaagtaact cctgttgctt tagttgaagc
    89281 tggaatcgtg aaaaacgaaa aagctggaat caaagtatta gctaatgggg aactaaacaa
    89341 gaaattaact gtgaaagcag caaaattctc taaagcagct caagaagcaa ttgaagcggc
    89401 tggtggatca atcgaggtga tctaatgttt agactattaa aagatgcttt taaagtaaaa
    89461 gacattagat cgaaaatcct atttactgta tttgtgttgt ttgtatttcg cctaggtgca
    89521 catattactg taccaggcgt aaatgctgct tcattgcaaa acctttcttc tttgccattt
    89581 ttcaacatgt tgaacttggt aagtggtagt gcgatgcaaa acttttccat tttctcaatg
    89641 ggggtttcac catacattac tgcttctatc gtcattcagc ttcttcaaat ggacatcgtg
    89701 cctcgatttg ttgaatggtc gaaacaaggg gaagttggtc gtaaaaaact gaatcaagcg
    89761 actcgatatc tgacattagt attggcattt atccaatctg tcggtatcac tgctggattc
    89821 aatcaatacg ctcaattagg tttcgtgaat aatcctaacg tagcaactta cgtaacgatc
    89881 ggtttgattc taacagccgg tacgatgttt gttacttggc taggagaaca gatcacagaa
    89941 aaaggtatcg gaaatggcgt atcaatgatc atttttgcag gtatcatttc tagattgcct
    90001 gaaagtatca gagaaattta tgaagattac tttataaata ttgatccttc agacatatgg
    90061 aaatctgcta tttttgtggc cattttacta gtagcggtct tgttgatcgt gacattcgta
    90121 acatttttcc aacaagctga acgcaagatc ccgatccaat atacgaaacg cgtagctggc
    90181 gcgcctacaa gcagctatct gccgttgaaa gtaaatgcag ctggagttat tccagttatc
    90241 tttgctagtt cgtttattgc tacgccaaat gctgttttac aagcattgag cggtagattc
    90301 ggttctgaaa actggtacaa agtgatgatg gagatcttta actataatac agcacctggt
    90361 gctacgattt atacgattct gatcgttgcc ttcactttct tctacgcctt tgttcaagta
    90421 aaccctgaga aattagcgga aaacttgcaa aagcaaggaa gttatatacc aagcgtacgc
    90481 cctggtaagg ggactgaaga gtatgtctca agattactga tgcgtttaag tacagttggt
    90541 gcagtcttcc ttggtttagt ggcattgctt ccaatcattg ctcaaatggt ttgggatctt
    90601 ccacaatcaa tcggattagg cggtacaagc ttactgatcg ttatcggtgt agctttagaa
    90661 actgcgaaac agttagaagg cttaatgctg aaacgtaaat atgttggatt cattgagtaa
    90721 gaaagcgtgt tgaggtttcc cctcaacaca cgttctcaca catttattag gagggaaaac
    90781 aatgaacctc attttaatgg ggctgccagg cgcaggtaaa ggaacacaag ctgaaaaaat
    90841 cattgatgct tatggtattc cgcacatctc aacaggagat atgttccgcg cagcgatgca
    90901 aaacgaaaca gctcttggcc tggaagcaaa gtcttatatg gataaaggtg cattagttcc
    90961 tgatgaagta acaaatggaa tcgtgaaaga gcgactagct gaaccagata cagagaaagg
    91021 cttcttatta gatggctttc cacgtacgct agatcaagca aaagctctgg acgcaatgct
    91081 aaaagatttg aataaaaaaa ttgatgctgt catcgatatc catgttggtg aagaaatctt
    91141 ggtcgaacgt ctagccggcc gatttatctg cagcaattgt ggagcaactt accataaaat
    91201 tttcaaccct acaaaagttg aagacacatg tgatcgttgc ggtgggcatg aattctatca
    91261 aagagaagat gataaacctg agacggttaa aaatcgtctg gctatcaaca tcaaaaatag
    91321 tgaaccaatc ttggcttatt ataaagaaca aggtttgcta aacactattg atggtgctcg
    91381 tgaaatcgat gctgtatttg cagatgtaca aaaaatcatt gaaaaatagc acgattgccc
    91441 gtagccgact tgcattatgc agttactctt gtcaagttca ataaattatg atagaatatg
    91501 tcagtccgac ttctagagaa tcctctagat gatccatttt gagaattttg aggtgggaac
    91561 tgcagtttcc gccattttat cgtgtgaaaa tgcgaaacaa gtacaaggag gtactgtgcg
    91621 tggcaaaaga agatatgatt gaagtcgaag gtacagtcgt cgaaactttg ccgaatgcaa
    91681 tgtttaaagt tgaactagaa aacggacacc aagttcttgc tactgtttca ggtaaaattc
    91741 gtatgcatta catccgtatc ttacctggtg acaaagtaac ggttgaattg tctccatacg
    91801 atcttaaccg tggccgtatt acttatcgct ttaaataatt gtactccgta aaactacagg
    91861 aggtattatc atgaaagtaa gaccatcagt aaaaccaatg tgcgaacatt gtaaagtaat
    91921 tcgtcgtaaa ggacgcgtta tggtgatttg cccagcaaat ccaaaacata aacaacgtca
    91981 aggataattg gaggtgctat aaatatggct cgtattgcag gagtagatat tcctcgtgat
    92041 aaacgtgtag tagtttcact tacttatatc tatggtattg gtaacactac tgctaaaaag
    92101 atcttagctg atgctggtgt atctgaagat gttcgcgttc gtgaattaac gaatgaacaa
    92161 acagatgcta tccgtgcaga agtagataaa ttaaaagttg aaggggatct tcgtcgtgaa
    92221 gtgaacttga acatcaaacg tttgatggaa atcggttctt accgtggaat ccgtcatcgc
    92281 cgtggattgc ctgttcgtgg acaaaacacg aaaaacaatg cacgtactcg taaaggccca
    92341 gctcgcgcta tcgctggcaa gaaaaaataa ttcttaagtg aaggaggtta aaacttcatg
    92401 gcagcaaaaa aagtgaatcg taaacgccgt gtgaagaaga atatagaagc aggtattgca
    92461 catatccatt ctacattcaa taacactatc atcatgatca ctgatactca cggaaatgca
    92521 ttagcgtggt catcagctgg ttcattaggt ttcaaaggaa gcaaaaaatc aactcctttt
    92581 gccgctcaaa tggcagcaga aactgctaca aaagcagcaa tggaacacgg attaaagact
    92641 gttgaagtaa cagttaaagg acctggttct ggacgtgaag cagcaattcg ttcattacaa
    92701 gctacaggtt tagaagtgac tgcaattcgt gacgtgactc cagttcctca taatggtgcc
    92761 cgccctccaa aacgccgtcg tgtttaatga gtgccgctca ttttgagttt tatcagtaac
    92821 gtcattgaac aagacacttt tcgttttgaa aggggtaaag ataagaatga ttgagttcga
    92881 aaaaccaaga atcgcaaaaa ttgatgaaga aaaagattat ggcaagttca tcgttgaacc
    92941 tcttgaaaga gggtatggga ctactttagg gaattcccta cgtcgtattt tattatcttc
    93001 tttgccaggt gctgccatca ctaatattca aatcgatggt gtactgcatg aattctctac
    93061 catcaaaggt gttagagaag acgtcactca aatcatcttg aacattaaag gcttagcgct
    93121 gaaaatgtat ggccaagaag aaaaaaccct tgaaatcgat attaccggac ctgctacagt
    93181 aactgctggc gatattatcg ttgatagtga tgtagaaatt cttaacaaag atatgtacat
    93241 ctgtagtgtc tctgaaggag ctacgttcca tgctcgctta acagtgaaac ctggacgtgg
    93301 ttatgttcaa gcagatgaaa ataaaaaaga agatatgcca attggcgttc ttccagtcga
    93361 ttcaatctac acacctgttc gtcgtgtgaa ctatcaagtt gagaacacac gtgttggaca
    93421 ccgtgaagat tttgataaat tgacgatgga gatttggaca gatggttcaa ttgaaccgtt
    93481 ggaagcaatg agtttgtctg cgaaaatcat gactgaacat ttggatattt ttgtgaactt
    93541 aactgatgaa gcaaaaaatg ctgaaatcat ggtcgaaaaa gaagaaacac aaaaagaaaa
    93601 aatgttggaa atgacgatcg aagagttaga cttgtctgtt cgctcttaca attgtttgaa
    93661 acgtgcagga atcaatactg tacaagaact tacaaataaa tctgagcctg aaatgatcaa
    93721 agtccgtaac ttaggacgta aatcattaga agaagttaaa gcaaaattgc acgacttagg
    93781 tttaggttta cgtaaagaag attaaaacct tttacgaagg agggaaacca acgtgagtta
    93841 tcgtaaacta ggacgcacat ctagccaacg taaagcgatg ttgcgtgatt taacaactga
    93901 tttattaatc aacgaacgta ttgtgactac tgaagctcgt gcgaaagaag ttcgctcaac
    93961 tgcagaaaaa atgatcactt taggtaaacg cggagatttg catgcacgtc gtcaagctgc
    94021 ggctttcgta cgtaatgaag ttgccagcgt gcgtgaagaa aatgaggaca tcgtcattga
    94081 atcagcttta caaaaactgt tcaacgatat tgcacctcgt tacgcagaac gtcaaggcgg
    94141 atacacacgc attttgaaga cagaaccaag acgcggtgat ggtgcaccaa tggttgtctt
    94201 agaacttgtc taaaatcaca ctaataacat cactttatag atgattcttt tagagtgtta
    94261 tgatgttgga agatattttt ccgagtctag ctcagcgctg ctcctcaagc gaatatcgct
    94321 tggggagtaa gcactcatca taaagtgttt ttttatacct tcaattacct gtcatgacag
    94381 atatgtttga aggcataaaa aaacatttta agcgagaaat aattgaaaat tttgtctttt
    94441 tttgatagtg ttagtaggaa ctttaggatg aagaaggagt aatcatggag ccaattattg
    94501 aattagaaaa gataaattat aaataccagc cagatgatct tcgccccgca ttgaaagatg
    94561 tctcctttac aattgataaa ggtgagtgga tcgctattat tggacacaat ggttcaggaa
    94621 aatcgacact tgccaaaacg atcaatggat tgcttctgcc agaatcaggg atcgtgaaag
    94681 tgggaaatca aatattggat gaagaaaata tttggacgat ccgacagatg gttggaatgg
    94741 tttttcaaaa tccagacaat cagtttgttg gttcgacagt agaagatgac gttgcttttg
    94801 gccttgaaaa tcaagggatt cccagagaag aaatgctagt gcgtgtgaaa gatgcacttg
    94861 agaaagttag aatggctgag tttgcttcac gcgagcctgc tcgtttatca ggagggcaaa
    94921 aacaacgtgt tgccattgcc ggcgtggttg ccttacgacc tgatatcatt attttagatg
    94981 aagcaaccag tatgctagat ccagaaggtc gtgaagaagt gatctctacg atcaagaaga
    95041 tcaaagaaga aagtcaattg accgttatat ctatcactca tgatattgat gaagcagcta
    95101 atgccaatcg gatactagtg atgagacaag gcgaacttgt ccgtgaaggt acaccaaaag
    95161 aaattttttc tgctggtcct gaactgatcg acttaggtct agatctacca tttcctgaaa
    95221 aactgaagag cgcactgaaa gaacgtgggg ttgatgtgcc cagtgaatat atgacagaag
    95281 aaaggatggt ggattggtta tggacatccg ttttgaacaa gtagacttca cttatcagcc
    95341 gaatacacca tttgaacaaa gagccttatt tgacatcaat atgacgatca aagaaaacag
    95401 ttatactgca ttagtagggc ataccggaag cgggaaatca accttacttc agcatttgaa
    95461 tgcgcttgtt aagccaacaa gcggtacagt ccatattgga gaacgagata ttcagccgga
    95521 tacggataat aagaatttaa agcccatccg aaaaaaagtt ggcatcgtct ttcagtttcc
    95581 agaagcacag ctattcgaag aaacggtagc aaaagacatt gctttcggtc ctaaaaactt
    95641 tggtgtcagc gaagaagaag cactagtcct agcaaaagaa acattagaac aagttgggct
    95701 ggatgaaagc tatttggaac gttcgccatt tgaactttca ggagggcaaa tgcgtagagt
    95761 agcaatcgct ggtgtgcttg ccatgaggcc ggaagtactt gtattggatg aaccaacggc
    95821 aggacttgat ccccagggca gaaaagaaat gatggagatg ttttggcgtc ttcataagga
    95881 acagcagatc actattgtat tagttacgca tttaatggat gatgtagcca attttgcaga
    95941 ttatgtctat gtgttggaaa agggacgaat cgttaatagc ggggagccgc aagaggtctt
    96001 tcaaaacatt gaatggctaa aagaaaaaca actaggcgta cctactgcca ccgagtttgc
    96061 agaagaatta atggctaaag ggatgaactt tgctaccttg cctttgacag ctgaagaatt
    96121 agcagacgct atcgtccatt atgctggagg aaatgcccat gatgaataaa ttgatatttg
    96181 gtcgttatat gcctggagat tccttgatcc atcgattgga tccccgagct aaattattag
    96241 ccagttttta ttttatcgga atcatttttt tagcaaataa ttgggaaacg taccttttgt
    96301 tggcattttt cacattactt tctgtcttct tgtcaaaagt agatttgaaa ttttttatcc
    96361 gaggcattcg cccattgatc tggctaatcc tcttcactgt agctttgcag gtcttgttta
    96421 caagtggcgg agaaacttat tggagttggg gtgttttcca tatcactgaa tttggtgtga
    96481 tgaacggcct ctttattttc tgtcgattcg tgttgatcat ttttatgtcg actctactca
    96541 cgcttactac accgcctttg gaattatcgg atgcgattga gtacttgctt cgtccattga
    96601 aagccgtacg gttcccagtc cacgaaattt cattgatgct gtcgattgct cttcgttttg
    96661 tgcctacatt gatggacgaa acagaaaaga tcatgaacgc tcagcgcgct cgcggtgttg
    96721 attttggtga aggaaaccta gttcagaaaa tgaaagcagt cattcctttg ctgattcctt
    96781 tatttgtcag cagcttcaac cgtgcggaag atcttgcgac tgctatggaa gcaagaggct
    96841 acaaaggtgg ggaaggaaga acgaaatatc gtgtattgca ttggcataat agagacacga
    96901 tggttatcat tgcttttgta ttattaactg tgctactgat ctttttaaga ggataaacgt
    96961 tcagtgggcc gctatctatt cttttcttgc tgatctgttt gcaagaaagg atgcaagcag
    97021 tgtatgacaa gaaatattag ggtctgtggc agactaaggt cacaggcctc tttcttttat
    97081 ctgaaaagaa agaacgaaaa aattttatag aaaaatcatt attccagata accttttagg
    97141 aacaaaggag agaaaaacat ggtacgttac aaagcaatca ttgcgtacga cggaacaaat
    97201 tttaatggat tccaaaaaca gccaaatggg cgtactgttc aagaagaagt agaaaaaaca
    97261 ttacaaaaaa tggcaaatgg aaaagaaatc acggtctttg gttcgggaag gacagatgcc
    97321 ggagtacatg cgatcgggca agtaatccat tttgattatc cagaggagcg tccgttagaa
    97381 cgaatgcgtt tcgcgctaga tacccagtca ccagaggata ttgctgtaag acaagtagaa
    97441 attgtttcag aagacttcca cgcaagatat ttggtcaaag aaaagacgta tcaatttcga
    97501 gtggatatag ggaaaccacg cagtcctttt cgacgccatt atgcaagtta ttttccttac
    97561 ccacttgatt tggaaaaaat tcagcgggcg cttcctgatt tattagggac tcacgatttc
    97621 acctcttttt gtgcatcagg aagctcgata gaggataaaa tacgtacgat ctacgaggct
    97681 aagatggagg taaatgaaac aaaagacgaa ttgttgttta catttagggg aaatggcttc
    97741 ttgtataaga tgatacggat cttagtagga acgcttttga aaattggcaa tggccgattg
    97801 cctgaagata gtatcccaga tattattgcg aaaaaagatc gtaatgcagc aggacctaca
    97861 gcacaccctg aaggacttta tttgtatgaa gtgaaatatg aataagaggt tatgatagaa
    97921 gtggtcagct tcaagaaata agaaggtatt tacgaaaatt acttctcaaa attttgcaaa
    97981 atttcggctt attttcgaag aagctacttc tggaacaccg tttaaggaag aggtcgtgaa
    98041 atcagcgttt tgacctgagt attagagaaa caaacgggaa aatagctcct catattttgc
    98101 cgtttgttga gctaataccg aaggtcagct gattgaacac cgtttaagaa agaggtcgtg
    98161 agcctgtcat ttaactctga gtcacaaaga gcaatttcaa aaaacagctt ctcatctttg
    98221 atgaatatcg tgacttgtgg acgaggcgtt gataagtgaa tcatcttttt gttacctatt
    98281 ttttctgtaa ccaaaaaacg atggaacgtt gttttgattg cccttggtgc ggacagtctg
    98341 aacaggttcc atcgtttctt tattttctac gtcttcttct gtgtgtgttt cgtggttgtt
    98401 cttctattgc tggatcaatc gtgccataaa aggggcgatt gcggtcaagg taccagacat
    98461 aaagaataaa caatgcaata cataggacaa aaaggattgc acctatcgta aatccttcag
    98521 gcaagtaact gaaagtgaca gtatgtttac ctgctggaac ttttatcatc aagaatgcgt
    98581 ctttgaaatt gctgaccttg actggtttcc catcaatttt agctttccat cctttgtcat
    98641 aaggaatagt tgtcaccaac atggaatctt cagcggtatt tacagtccca gaagcttttc
    98701 tgccagtggc ttccatttcg acaccttttt ctttgatctg attcattgcg gcttgatagg
    98761 ctacaacatc tagaccaacg actttaggct ccatgaaact tatttctttc gtcccataaa
    98821 aacttgcagt aaatgtcact gttgtctctt gaggataata gccaagatca tagtattgac
    98881 cagaaatgtt gatttgcgtt tttcgcgatg taccattgac agtgatcgtc gcactagaac
    98941 tttctaattg cgcaaaattg gttgggaaca aacttaagta agcttgtgta tttgcaggca
    99001 catggacttc ccaagtgagc tcttttggct ggttatctgc tgtttctgag taagtgacgc
    99061 cagtcgttgt ctttgtgatc gtcgtattca actgattctt caaagtaggt gtatagaatg
    99121 taaaatactg ttcatttaga ccagacaatg tattgatcaa gtttgtctga ctggtcaaat
    99181 tatctgtcac tggctgctca acatcattaa cggacaacgg tgcagtaaat gctagtggca
    99241 atgcattgac attctgatac agtgtatagt cgccgctttg accagcttgg cggaagccat
    99301 atttactgaa cgtgctttcg tttttagaaa tgttatattt gattcctgta aacccatcca
    99361 tcagcaaagt attattgtta taacggatat tcaaatttgt ccctcttgaa cggaaaccta
    99421 atttatccag gtaagaggaa gaattacgat ttcttataga agaaaacaaa ctgataccac
    99481 tgtaaccata gttgatcgaa tcgtttgcag atactggatc cagattttcc aagcggtaaa
    99541 acgagtcgga atcttttttt gtttcattta caagagcttt caagtcagga taaggatcag
    99601 tatataaact gcgggaagca taattccaat catcaagaat gccatggatc atactgttcg
    99661 tattgaccag tgcttcagca gacatgatca ataataacaa tatactcaga tgattgaatg
    99721 aaagtttttt atgctgataa aatcccacag caaaagcata aatccctaag aaaaccactg
    99781 tcaatacaaa ggaagtcatc gtcacatagg tgtagcttcc ttcaggtttg atgccccatg
    99841 ccaccgcaaa gagagcagct acgaccaaaa tgattcctaa caacagccca cgatcgtctt
    99901 gttctatctt ttcccatcct tttcctgcaa gcaggagcac taagaaagag aacagatagg
    99961 cataacggaa taaaaacata ttcggtgcat gcataccatg ccagaacaaa ttcaatggaa
   100021 caagataaaa acttgcgata agtatggcga aaagactgcc gtacaaaaat ttatttttca
   100081 acggtacttt ccgactggca aaataaaaca gacagaggac taatggtagc agacctgcgt
   100141 aaacgaacgg aatcgatccg tattttgtcg tgtcataaac accaatcata tttttcatga
   100201 ttagatccag ataagaagtc gcttctgttt tcaacgtagt gatctgactc agtgcctcgc
   100261 catttgaccg aagatccagc actgctggca atacaatgat cattgaagcg ccgcctgcca
   100321 gtaaagaagt gataccataa ggaacaatcg tttttttata ttctgaccag tttgtcaata
   100381 gacgggcgat aaaatataag aaagaaaata cgccaatcat aaaacccata taaaaactgg
   100441 ataaaaacaa gagtaaataa ctgataaata aaagtttcgg ccgtttaaga tccataacac
   100501 gatgaatccc caaaatcact aatggaagat aaaaaaatgc atccagccac atgatgatct
   100561 ccgaatgtgc agtgataaat gagatcaatg cgtaactgac gctaagtgca acatgccccc
   100621 agcgaggaat cttgaacgta cgtgcaagaa accaaaaagc caatccggca cagccgacct
   100681 taagcaaagt cagaacatac agtgcatctg gcatcatctg gtttggaaaa aagaacacca
   100741 gtggcgtgaa gatcccgcct agatagtaag aaattaaaga taaataattc aatcctagag
   100801 aagcattcca tgtataaaaa atactttgct ttccaagaag aacatttcgg aagctggcat
   100861 gaaaattaga aaactgtgaa aaggcatcac ttgccaaaat acttctgctg cttccaggat
   100921 agattccgat acttagataa acaagtgcta aaatcaaaaa cggaatcagg aaactagctg
   100981 ctgcgtagcg tccgttttct ctaaagaatg actttaaatt ttttttcata tttattttcc
   101041 accaaatttc tatgaagttg tattcagttt atcatatcct tgtttttctc tgcttaaaaa
   101101 gagtctagtt ttcacaaaaa aataaaattt agaataaata acttcataat ttgttcgtat
   101161 aacaaaaata aacgcaaaaa aagggtagga atcaaaagtc ccacccttaa aaatttttat
   101221 taaataaaaa accgttcact atctgaacgg tccaagtatc attcaattaa acgcgttcaa
   101281 cttttcctga tttcaaagca cgagttgaca cccaaacttt tttaggtttt ccgtcgacca
   101341 taacgcgaac tttttgtaag ttaggtttaa cagtacgttt tgttgagttc atagcgtgtg
   101401 aacggttgtt tccacttttt gtcttgcgac ctgtaaagta acaaactttt gccatggtaa
   101461 tttcctcctt tgccttgatt tggagcaata gaataattca gctcatacca gactaacata
   101521 tcatatagaa tgggaatttg caagtacatt ttcatagata caattgacaa ttgagaagaa
   101581 taattgtaaa ttaaacaagg ttatacctga aattttgcaa ggtttttgag tactttgggt
   101641 aactgccttt tatttcaagt tgcgctatga taaaatatga tagaagaaaa atgggagttt
   101701 tttacccgtt aaggaggctc attcatggct gtaaaaattc aaacgtcagc tggaaccatt
   101761 gaaattacca atgatgtaat tgcaacagtt gttggtggcg ccgcgacaga tgtgttcggt
   101821 gttgtaggaa tggccagcaa aaatcaaatt aaagataata ttactgagat tttgcgtaaa
   101881 gaaaattact caagaggagt agttgtccgc caagaagata atggtattgc tgtagatgtt
   101941 tatacgatta tcagctatgg tacgaaaatc tctgaagttt cccgcaacgt ccaagaaaaa
   102001 gtgaaataca atctagaggc attattaggc gtaactgcca attcagttaa tgtctttgta
   102061 caaggtgttc gtgtactgcc agactaggca ggagactggc tggtaactca gcagtcgatc
   102121 tgaaggagga ttatctaggt gagtgtaaca gaaataagtg caagccagtt ccaggaaatg
   102181 gtccaggcag gtgcgaatcg tctgcaagta aatgcagaat acgtcaattc attaaatgta
   102241 ttccctgtgc cagatggcga tacgggaaca aatatgaatc tatctatgac tagtggagct
   102301 aaggcagtag tagattcaac ctcagaaaaa gtaggagaat tagcagcaat gctttcaaaa
   102361 ggattgctga tgggtgcgcg cggaaattcc ggcgttattt tgtctcaatt gtttcgtgga
   102421 ttttctaagc aaattccaga tgttgtctca ttaaatgctt ctgatcttgc tgcagccttc
   102481 actcatggag ttgaaactgc gtataaagca gtaatgaaac cagtagaagg aacgattttg
   102541 acagtagcta gagaagcagc gaaagctgga gaaaagaaag caaaaagtac tgatgatgtc
   102601 gttgaagtaa tgacagctgt tgtaaaagga gcaaagcgtg ctttagctaa aactccagac
   102661 ttgcttccag tcctaaaaga agtcggtgtc gtagacagtg gagggcaagg actgctgttt
   102721 gtttatgagg gctttttaag tgcattgaat ggagaattcc aagcggatga aacatatgaa
   102781 ccttctccag cagaaatgga tgatatggtc aatgcagaac atcatcgcag tgtccaaggt
   102841 caaatggcga cagaagatat ccattttggt tactgtacag aaatcatggt aaaaatcgga
   102901 gaaggaccta cagtcgatag tacattcgat tacgaagaat tccgtaacta tttagatggt
   102961 ataggagatt ctttacttgt ggtcaatgat gacgagatca tcaaagtcca cgtgcataca
   103021 gaaaacccag gagaagtctt gaattatgga caaaaattcg gttcattagt caaaatcaaa
   103081 gtcgacaaca tgcgcttgca gcatgagacg attttggaac acgatgaaga agtgaacgag
   103141 tttgcgaaaa aagaaatacc acgtatcccg tatggtatcg tagcgatcgc tgctggtaaa
   103201 ggcgtccaag agctgtttga aagtttaggc gctaattatg ttatcagtgg tggacaaaca
   103261 atgaatccaa gtacggaaga cattgttaaa gcgattcaag aagtaaacgc agataaagtg
   103321 attgtcttgc caaacaataa gaacatcttc atggcagctg atcaagcagc agaagtagca
   103381 gatatcccag tagcagttgt tccttcaaga acgatttccc aaggattgac agctatgctt
   103441 tcattcaatg atcaagcttc tttagaagaa aacaaagaaa cgatgacaga aatgttgtct
   103501 agcgttgtga gcggtcaagt cactcacgcg atcagagata cctcaattga cggtgtagag
   103561 atcaaagaag gagacttttt gggcatgatc gatggaaaaa tcctgatctc taacccagat
   103621 atcacagcaa cagcgttaag tacactagaa caaatgatca atgaggacac agagatcgta
   103681 acgatcttgg caggtgatga aggaacagtt gaacaagcaa atgaatttgc tgagaaacta
   103741 acagaaaaac atcctgattt ggaaatcgag atccaccaag gagatcagcc tgtctatcct
   103801 tatctatttt ctgctgaata atttctgtgt cagaatatct ttctaacaca gtattcattc
   103861 ataaaaggcc ttcggttagt gatcaggtat gatcgctgac caagtccttt ttttgttaaa
   103921 ggagggagga aaaatttatg cgttctttag atgatccaat cactatgctg gcaggagtcg
   103981 gagaaaaaag agcagctagt cttgccagct tgggcatcca aacgattgaa gacttactga
   104041 cttattatcc ttttcgctac gaagatattc aggaaagaaa tctaaacgag atccaggatc
   104101 aagaaaaggt tactttgaaa ggaatcgtag tctcgcctcc tgtgatgaat cgatttggtt
   104161 ataaaaaaag ccgattgcaa tttcgtatga tgcaagaaca tgatgtgttc aacgtttctt
   104221 tttttaatca accttatcta aaagaaaaag tcattttatc ggaggaaatc gccgtatacg
   104281 gtaaatggga tgcaaagcgt aaagccttga acgggatgaa gattttaggt tctcaaacaa
   104341 tggatgactt ttcgccgata tatcacgtca acaaatcaat tcgtcaaacg acattagttg
   104401 atctaatcaa aaaagggttt caagaatacg gcacattgat tgaagaaaat ctgccaaaca
   104461 accttgtgga gaaatatcga ctgcttgacc gagcttctgc tgtaagaagt atgcattttc
   104521 caaaagacca cgaagaaaac catcaagcca aacgacgtgt cgtttttgaa gagttctttt
   104581 tatttcaaat gcgtcttcaa ggattgaaaa aagcagaaaa agcagaaacg aatggtttgg
   104641 agatccttta tgatgtccaa aaattgaaac aatttactca aaagctacct tttgaactga
   104701 caaatgcaca aaaacgtgta acgaatgaaa tctgtcggga tcttcgcagt ccgcagcata
   104761 tgcagcgact tttgcaggga gatgtaggta gcggaaaaac agtggttgcc gcgattgcgt
   104821 tatacgctac cgttacagca ggtttccaag gtgcgttgat ggtgcctaca gaaatcttgg
   104881 cacaacaaca tatggaaagc ttgcaacagc tgtttgatcc caatgaagta accatcgcat
   104941 tgattacggg gtcaacgaaa gcaaaagaac gacgtgagct ccttgaaaaa ctagagcaag
   105001 gggaaattaa cgtcgtgatc ggcacacatg cattgatcca agatggagtc gaatttcaga
   105061 atttaggtct agttattacg gatgagcagc accgttttgg cgtgaaccag cggaaagtac
   105121 ttcgtgaaaa aggctggcga ccagatgtct tatttatgac agcgactccg attcctagaa
   105181 cattagctat tacagcatat ggtgagatgg atgtatctat cattgatgaa cttccagctg
   105241 gacgtatccc gatcgaaaca cgctgggtaa gaacacctca attggacagt gtactggatt
   105301 ggacgaaaaa agaactggca agaggacatc aaatgtatgt catctgtccg ctgattgaag
   105361 aatcagaagc attagatgta aaaaatgcag tggaaatcta tgaaaaactt cgtgaactat
   105421 ttgcaccaaa atatgaagtt ggcttgttac atggtaaaat gaagaatcag gaaaaagaag
   105481 taatcatgga agccttcaaa gacaaccaga tgcagatatt agtttcgact actgtaattg
   105541 aagtaggggt aaacgtgccg aacgcgaccg tcatgctgat catggatgcg gatcgatttg
   105601 gattggcaca gctgcaccaa cttcgtggac gtgttggacg cggaagtgat gcttcctact
   105661 gtatcttagt tgctaatcct aaaaatgaac taggtgtgga acggatgaaa atcatgacag
   105721 agaccaccaa tggttttgta ctaagcgaaa aagatctgga attacgtggt cctggtgaag
   105781 tatttggctt ccgtcaatct ggtctgcctc aatttgcgat tgctgacttg gtgacggatg
   105841 gaaatgtttt ggaggtagcc agagaagagg ctgcagccct ttggcaaata aaggattggc
   105901 agatgcttcc tgagtatcag cctttagcgc aaagcctcca aatagaaaat caggataatc
   105961 gttatttcga ttagcagact gtgctatact ttatactgat gacggaaaaa ggagtggaaa
   106021 caatgaaaat tgccgtagat gcaatgggcg gagatcacgc acctcaagca attgtagaag
   106081 gcgtggcttt agcgcagcaa gattttccag aagtagaatt cttactttac ggaaaagaag
   106141 cagaaatcaa gaaatactta acaaatgaaa aaaacatcac gattatccat acagacgaaa
   106201 aaatcaatag tgatgatgag ccagtaaaag caattcgacg aaaaaagact gcatcaatgg
   106261 tccttgctgc acaagcagtg aaaaacggtg aagcagatgc tgtattttca gcagggaata
   106321 caggcgcgct actagcagca gggttgttca tcgtaggacg tatcaaaaat atcgaacgcc
   106381 ctggcttgat gtctaccttg ccagtagtag ggaaagaagg tgccggtttc gatatgctgg
   106441 atcttggcgc taacgcagaa aacaaaccag aacatttgtt gcaatacggt attttaggtt
   106501 ctttttatgc acgtaaagtc cgaggtatcg gacgtcctcg tgtcgcactc ttaaacaacg
   106561 gaacagaagc aacaaaaggt agtgaagtga cgaaaaaagc ctatgaactc cttcagaatg
   106621 aaacaagctt gaattttatt ggaaacgtag aagctcgtga gctactcaat ggtgtggctg
   106681 atgtagtggt cacagatgga tttacaggca acgctgtttt gaaatctatt gaaggaacag
   106741 caatgaatat gatgaatcta ttaaaatctg ctattctaaa tgaagggatc aaaggaaaaa
   106801 tgggcgctat gcttttaaaa gacggcttac gttctttgaa agcagaaatg gattattcca
   106861 aacatggcgg tgccgttttg tttggcttga aagcaccagt cattaaaact catggagcaa
   106921 caggccccga tgctgttcgc tatacgatcc gccaaatcca cacaatgctt gagacagatg
   106981 tagttggaca attagtcgaa caatttgaaa aaagcgaaga atagtatttg gatatctgaa
   107041 gcgatccgtt ccattgaaaa aaaggtggac attcctgaaa aatgccagta aaattagcca
   107101 tggtgtaggt aattgacaag tgtatgattc ttggaggtga aaaagttgac tcgcgaagaa
   107161 gtatttaaca aagtagcgaa aattatttca aatcattttg agatagatac cgataaagtg
   107221 acggatgaat tgaacatcaa agatgattta aaagctgatt caattagtat tatggaattc
   107281 gttttagagc ttgaagatga attcggtacg gaaatttcag atgaagacgc tgaacaaatt
   107341 gaaacagtcg gtggcgcagt agattatatc agcagccatc ttaaataatt tcaaataaaa
   107401 gcaaagaagg ataaaaagct gcatgcagct ttttatcctt ctttttttaa tctttttgac
   107461 acgtaaaagt catttttctg ataattctgg cgataaacag aaattatgtt gcaaagtaaa
   107521 gcggttttct atataattat taataattca ttacacaaag ataagaataa cctttgttat
   107581 aaaacgattt attctgtgta atgtcctttt ttattaagaa aaacttccat ttaggaagca
   107641 gagaggggta ttcgattgtc cgataaccaa ttattagaag ttcagaacct acatacagga
   107701 ttccgactca aagatgacta ttacgatgca gtagatgacg tttcatttac tttagacaaa
   107761 aacgagattt tagcaatcgt tggggaatct gggtgcggga aaagtacctt ggctactacg
   107821 attatgggat tacttgatcc taataacaca aaaattacag gtgagatttt atataacgat
   107881 ctgaatttaa tcgacctgaa cgaaacgctc tataataaaa tccgcggaaa tgatatcgga
   107941 atgattttcc aagatccgtt gtctgcatta aatcctctca tgagaatcga agaccagatc
   108001 aaagaaggac tttcctatca cacaaagatg aatgcagaac agcgtcaagc tcgtgcacta
   108061 gaattgctag aacaagtagg gattcctaat cctgcgagag ttggacgcca atatccccat
   108121 gaactatcag gcggtatgag acaacgtgtc atcatcgcaa tcgcaatcgc ctgcaaacct
   108181 ccaatcgtca ttgcagatga accgacgaca gccctagatg taaccatcca agcacagatt
   108241 cttgatttac tgaaagacct tcaggaagaa acaaaaacag ggatcatctt gatcactcat
   108301 gacttaggag tcgtagcaga aatggctgat cgagtggcag tgatgtatgg cggacagttc
   108361 gttgaagtag caggtgtaaa agaactgttt gatcatccta agcatcctta tacacgctct
   108421 ttactgaact ctataccaca agaaggaaat catgatgcag aactccatgt gatcgaaggt
   108481 gtcgttcctt ctctgaaaaa catgcctcgt aaaggttgtc ggtttgcacc acgtattcca
   108541 tggataccag caagcgatca tgaagaagag cctatccttc atgaagtcgc accgaaccac
   108601 ttggtacgtt gctcctgcta taagcatttc cactttagag acgaaaaagg agaggtgtag
   108661 atgacagaat taatcacaat caaagacctg aaagttcatt accctattcg cagtggattc
   108721 ttcaatcgag taacggatca tgtatatgct gtagatggtg tggattttat tatcgaaaaa
   108781 ggaaagacat atggtttagt gggagagtct ggctctggaa aatctactac gggaaaagcc
   108841 gttgtgggat tagaaaaggt aacagccgga caaatcatgt atgaaggaaa agatgtaacg
   108901 aagcgaagca accgcaaaaa aatgggttat aacaaggatg tgcagatgat cttccaagat
   108961 tcaatgtcca gtttgaatcc taaaaagcga gtgttggata tcattgctga acccatccga
   109021 aattttgaac ggttaagcga tcaagaagaa aagaaaaagg taaaaggact gctagatatc
   109081 gttgggatgc cggaagatgc gctatataaa tatcctcatg aattttcagg cggacaacgt
   109141 caacgtctag gagtagcccg tgctgttgct accaatccaa aactgatcgt agccgatgaa
   109201 cctgtatctg ctctggatct atctgttcaa gcgcaagtat tgaatttcat gaaacgaatc
   109261 cagcaagaat acggcctcag ctatttattc atttcacatg atttaggtgt agtaaaacac
   109321 atgtgcgaca atatcgcaat tatgtacaaa ggtcgatttg tagaaatcgg tacaagagaa
   109381 gacatttata atgacccgcg acatatctat acgaaacgtc tgctttcagc tattccgcaa
   109441 atcgatgtag aaaataggga aagccacaaa gaaaatcgac gaagagtaga acaagagtat
   109501 atccaaaacc aaaaagaata ctatgatgcg actggtcgag tctacgattt gcgtacgatc
   109561 acaactacac acaaagtggc gttgaaagat ggaggtgcaa gctaatgtgg aaaacgattc
   109621 tgcgacgtgt tttattgatg atcccgcaag ttattatttt aagtgttctg atcttcttat
   109681 tagcccaagc gatgccgggg gatccattta ccggtttgat caatccaaat caggatccgg
   109741 cagtgatcga acagatgcga caagcagctg gcttgaatga cccatggtat gtccagtact
   109801 tccgttggat cggaaatgct ttgcaaggcg attttggtca aagtttctta tacaaacgct
   109861 ctgttgctga cttgatcggc gaacggatca tcaacacttt atatctatcg atcttgacag
   109921 ttgtccttac ttatttgatt gctgtgcctc tggggatgct tgctggccgg tatcaaaatt
   109981 caatcttgga taagattgtc gtaatctaca acttctttag ttttgctgtt cctttattta
   110041 tcttcggctt gatcatgcta tttgtttttg gttatcgttt aggctggttc ccaacaagtg
   110101 gatcacaaac actaggtttg caaggtggat ttatcactca atggatagac cgtttgaaat
   110161 atattctgtt accatcaatt acccaagcat ttttggcgac tgccgtcaca atccaatatc
   110221 tgcgaagtga agtaatcgat tcgaaatcgc tcgattttgt cagaacagcc cgttccaaag
   110281 gggtaccgac gaataaagta ttcagtcgac atattttccg aaatgcagcg ttgccgatgg
   110341 cttctcaaat ggggtatgaa atcacttcgt tgattgctgg ttctgtagtt atcgaaaaga
   110401 tttttggtta tccgggagtc ggaaaactat ttattgattc catcggccaa cgggactata
   110461 ccgtgatcac atccttagta ttgattttag gaatcgcgac gctagtgggg acattacttt
   110521 cagatatcat catgagtatc gttgacccgc gtattcggat tcaataaaac caatcagaaa
   110581 ggagaatgga tcgatgagtg aaaaaaacaa tacagcagtt caagaaagta ttcctcctat
   110641 ggggatacga atgattgctc gagaatttaa aaaagataaa attgctattt tttcattagg
   110701 tttgctagtg gttttattat tagtgatctt tatcggtgcg atgctgaccg atcaagacaa
   110761 agtgatgtat gttagtatct tcgacaaata cgctgcacca ggaacagttt cgacacaagg
   110821 acaaaagttc attctgggag cagatgaagg cggacgtgat gtattaggac aattgatcat
   110881 aggtgctaga aactctgtta cgatcggatt tgcgatcacg atccttactt cgatcatcgg
   110941 ggttggatta gggatcatct ctggctacta cggagggatc attgacaaca tcttgatgcg
   111001 gatcgttgat tttatcatga ttctaccgat catgctgatc attatcgtct tcgtatcgat
   111061 tatttccaga tacaatgtct ggtcctttat attcatcatg agtgcatttt attgggtagc
   111121 taaagcccga ttattccgaa gcaagacatt atctgaagca agaagagatt atgtcagtgc
   111181 ctcaaaaaca atgggaacca atgattttaa aatcatgttc cgcgaaatca tgccgaatct
   111241 aagttcatta atcattacta atttgacgat gaactttgcg gcaaacatcg gtatcgagac
   111301 aactttgact ttcttaggtt tcggcttgcc ggcaaatgta cctagtttag gtacattgat
   111361 cggttatgca agtaacggag aagtcttagt gaatcggcaa tggatctggt tacccgcatc
   111421 aatccttatt ttagttttga tgttgagtat caattatgtt ggacaagcat tcaagcgttc
   111481 tgcagatgcg cgccaacggt taggctagta tagaagcgaa gggggaaata ggaatgaaga
   111541 aaacgatttt tggatttgca gcgctgatgt ctgctgtcgt tcttgcagca tgcgggggcg
   111601 gaggtacaga tacctctgga aacaactctg gaggcaacgg caataacaaa gaggaattaa
   111661 cttttccaat tgcaacaaaa aataatgatg atgcaatcga agacggcgaa ttagaagctg
   111721 cggtcgcaac ggacagtcag tttaaaggat tgttccagtg ggagttttac caagattcat
   111781 atgatgcagc ttttatggct ccttctcatg aaccgctatt tgcaaacgat gacgatttca
   111841 gaatcacaga cgacggagct gcaacgcttg atctagatga agatacgaaa aaagcgacaa
   111901 tcaaagtaaa agacaacgtc aaatggtcag atggtgaaga tgtaacggcg gacgatgtga
   111961 ttttccctta cgaaatcatt gggaataaag attacactgg tatccgttat gacgacactt
   112021 tccgtaacat cgtaggtatg gaagaatatc atgatggaag tgccgacaca atctccggaa
   112081 tcaaaaaaat agacgacaaa acagttgaaa tcgaatataa agaagtaaac ccaagcatgc
   112141 ttcaatcggg tggtggtatc tggtcatatg cagcaccaaa acatacgtta gaaggtgtag
   112201 caatcaaaga tatggaatca agcgatcaag tacgtaaaaa tccagtgact tttggccctt
   112261 attacatgag taatatcgtt gcaggagaat cagtagaata cttgccaaac gagcactact
   112321 ggaatggtac gcctaaattg aaaaagatca cgatgaaatc tgtaccgatg gcaagtgcaa
   112381 ctgaagcatt gaactctaaa ttatatgata tgattttcca aatgccaaca gatacttacg
   112441 atacctataa agaaattgct ggctatacaa acttaggccg tgaacaaact tcttatactt
   112501 atctaggatt caagttaggt aaatggaatg cagatacaaa gagtgtggaa tacaatccag
   112561 attcaaaaat ggctgataaa tcattacgtc aagcaatggg ctatgcatta gataataatg
   112621 cagtaggtga acgtttctat gcaggtttga gaagcaatgc gacttcattg attcctccag
   112681 tattccagtc ataccatgat gatgagatga aaggattcac acaagatcta gacaaagcga
   112741 aaaaactatt agatgatgct ggctacaaag atgtggatga cgatggttta agagaagaca
   112801 aagatggaaa taaactaaca atcaactttg cttcgatgtc cggtggggaa acagcgcaac
   112861 cattggcaga ttactatatc caacaatgga aagaaattgg tttggatgtc caatacacaa
   112921 caggtcgtct gatcgaattc aattctttct atgataaatt agaaaacgat gatcctgaaa
   112981 ttgatgttta ccaagctgct tggaatacag gaactgatcc aaatccaagt ggactatggg
   113041 gaccaaatgc tgcgttcaac tacactcgtt atgaatcaga agaaaataca aaattgatca
   113101 acgatatcag ttcaagcaaa gcatttgatg aagagtacca aaaagaagca tatcaaaaat
   113161 ggcaacaata tgcatttgaa gaagcatttg cgattccaac gttgttccgt aatgaagtat
   113221 tgccagtcaa cgaccgtgtc aaagactggg attggtctta tgatgtacca aacccatggg
   113281 caaatgtttc tgttacagca gattcacgtt cataatcact aaaaatgacc gaaaaagggc
   113341 gatgtccttt ttcggttttt tgatattttt ctgtttattt ctctaatatt caggtcaagg
   113401 cgctgatttc atgacttctc caataaacgg tgttcaaagt ccagtttctc ggtattaagt
   113461 caaatttatg caaaaatatg gaaagccatt ttcacaaaat tttttcttca tactcgaaac
   113521 taaacgaatt tttcacaact tctcgtcaat cactaggatt attaaagctt tacttacctt
   113581 ttgaccgaac catttcaaat ttgacaaaaa accagtataa taaggaagta tcgaaaaaat
   113641 atccataaaa ggaatgtgga aaatggacaa ccagctgaca aaagaactaa aagaaaacta
   113701 taacatcgtt tttcacgatc taaatttgct tgaacaagct tttacccatt catcctatgt
   113761 gaatgagcat cgcaatctgc aattatcgga taatgagcga cttgaatttt taggagatgc
   113821 cgttttagaa ctgatggttt ctcagtattt attccgttta tatcctcatt tgccagaagg
   113881 aaaattgaca aaaacacgag ctgcgatcgt tcgcgaagac agcttgtcga aatttgccaa
   113941 agaatgtcac tttgatcaat atgttttgtt gggcaaggga gaagagaatt caggaggacg
   114001 tacgcgacca gcattgcttt gcgacctatt cgaagctttt ttaggcgcgt tatatcttga
   114061 ccagggattc gacacgaccc atgcattcat cgaaaaagtg atttttccta aagtcaaagc
   114121 aggtgctttt tcacatgaga tggatcataa aacaaaacta caagaagtct tgcaaaaatc
   114181 aggtgatgta tcaatcgaat accgattgat caatgaagaa ggtccagctc atgaacgagt
   114241 attttggatc gaagtttatg tagatgatca attgattggc actggacaag ggaaatcgaa
   114301 aaaattagca gaacaagctg cggcggaaaa tgcattggca gcactgtaaa agtaagtgag
   114361 ggaaagcttt gtatttgaaa cgaattgaaa tagccggttt taaatcattt gcagacaaga
   114421 cagtcattga ttttgaaaat agcgtcacag cagtggttgg accaaatggc agcggaaaaa
   114481 gcaatattac agaggctatc cgctgggtat taggagaaca atctgctaaa agtctgcgag
   114541 gcggaaaaat gccggatatc atctttgccg gttccgatac ccgtaagcag ctgaatattg
   114601 cagaagtcac agtgatttta gacaatagtg atcattatct gccattggag tacaatgaga
   114661 tcagtgtaac ccgccgttat cgacggactg gggaaagcga gtttttcatc aataaacaaa
   114721 gctgcagatt aaaagatatc caagaattgt tcttagattc tggattagga aaagaatcgt
   114781 tctcaatcat ttctcaagga aaagtagaag cgattttcag tagtaaaccg gaagaccgtc
   114841 gtgggatctt tgaagaagca gctggtgtct tgaaatacaa gcagcggaaa aagaaagcag
   114901 aacagaagct ttttgaaaca gaagacaacc tcagccgcgt ccaagacatc atccacgaat
   114961 tggaagaaca gttaactcct ttggccgccc agagtgaggc agcaaaggag tttttacggc
   115021 taaaagaaac actgactcaa accgatgtct ctttgatggt tgctgaaatc aagacagcaa
   115081 aaaaagattg ggacaacaaa caagcacagc ttgcgaaatt caatctagaa ttaggcaaac
   115141 tatctgagtc gatccaagaa caagaatcca ttttggcgaa gcagcgtaaa gaaaatgcgc
   115201 aagccgatcg tctgatcgaa aaaaatcagc aagtattatt agatctttcg gaaaaactga
   115261 agcagacaga aggacaaaaa gatgttttgc aggagcggac aaagcataca caaaaaagct
   115321 cgcaagaata tcaaacttca ttagcagaag cacaaaaaaa ggttaaacat tttgaaaagc
   115381 ttcaagaaag cttgatgaaa gcagcagcag aaaaagaaac agagatccaa aaagctgaag
   115441 caaatctcat aaaaacacag caagaattag aaaaatacca aaaatcaacg aaagaattat
   115501 tggcagaatt aagagatcaa tatgttgatt tgatgcaaga acaagcagct gtaggaaacg
   115561 aactgaagta tctagaacgt caatatatac aagaaacagc aaaaagcaag cagacgttag
   115621 ccaagcaatc ggaagtagaa gcatcagtag accgattgat cttacaaaaa gaagaattga
   115681 cacaaaagca ggcgcagcta aaatcatctt taactgaaac aaaagagaag ttagaaatga
   115741 tccaacaaaa cgggaaaaag ttccaagaag cattagcaaa agaacagcct aaaatgtatc
   115801 aattaatgaa tcaagtccag cagctacgtg ctcgtaagaa aagccttcaa gaaatccaag
   115861 aaaactattt tggtttttac caaggggttc gattggtctt gcagcataaa cagcagcttt
   115921 cgggtattgt cggagcagtg gctgaactga tcgatgttcc tgcagatttc acgttagcta
   115981 tcgaagcagc tttaggtggg gcagcccagc atgtcatcgt tgaaaatgaa aaagatgctc
   116041 ggcaagccat cacgtatttg aaacagcaaa gaggaggaag agcaacattc cttccactga
   116101 cgacaatcaa accaagacaa ttaccggctc atatattgac acaagcagca gctgtagaag
   116161 ggtttatagg gattgccagt gaacaagtca gttatcccga tcaaatccaa actgtggttc
   116221 aaaacctgct aggaacgatc cttttagcca aagacttaac tagtgccaat gcaatcgcgc
   116281 aaacgatccg ctatcaatat cgagtggttt ccttagaagg tgatgtgatg aatgccggag
   116341 gatccatgac aggtggagcg aataaacgag gaaaccaagg cagtttgttt gtacaaaatc
   116401 aagaattgaa acagttgact agtgaatttg aagaagcaga taaacaactc caagcacaag
   116461 aaaaaaaagt tcaggagctg caacaagaaa cagctcgttt agccgaagaa caagaagttt
   116521 tgcgtacacg aggcgaacaa ttacgttttg aagaacaaga agcgacaaat cagctacaaa
   116581 acatcataaa tgaacttgaa cgttttgaaa aagaaaagca aatctcaact tttgagacac
   116641 gtgaactgca gcagtttatc gaagattacg aaaagcaaac gaacgaattg aaagacaaac
   116701 aaacagatct tgaatcgcaa cgacaaaaaa tcgacgaaga aatcaaatcg ttaagtcaag
   116761 aaagcgacca gatggaagcc agaagagcac aagtacaaag tcaaaaagca caagaacaag
   116821 cagatcttgc tgtcttgaaa gagcagttta atcatctgca gatccagctc cgcggtgccc
   116881 gagtccaaaa agcagaggca accgaacgtc aagaagcgat agaaaagcag ttggcgacat
   116941 tgacagcaga cttttctgat catgaagtca cagaagaaag tttggaaaaa caaattaacg
   117001 aattatctgc tcagcgagaa acattaaaag ccgagttggc aaaagccaaa gagcagcgag
   117061 atagaaggca aaaggagatc gataagcttg aagcagtctt ggcagagcgt aatcgtgagc
   117121 aaaaagcacg actgtcggaa caatccaaat tagaagtcca aaaagaccga gctgaaatga
   117181 ttttagataa tcatttgacc tatctgcagt cagaatatca gctgacattt gagaaagctt
   117241 cccaagacta tcaagaaaca accgatatcg aagacagtag aacaaaggtt tcatccttaa
   117301 aagaacagat tgaaaaactg ggaccagtaa acttaaattc aatcgaacaa tacgaacaag
   117361 tcagtgaaag acatacattc ttagcaacac agcgagatga tctgttagca gcgaaaaacc
   117421 aattgtttga aacgatggat gagatggatg atgaagtgcg gacaagattc aaagaagtat
   117481 ttgaagctat ccgtcaagaa ttcaaaattg tctttccaaa tatgtttggc ggcggtaaag
   117541 ctgaattagt actgacggaa ccaactgatt tgctgaaaac aggtattgag attgaagtac
   117601 aacctcctgg caaaaaactg caaagtctaa gtttgctttc tggaggagaa cgagcactta
   117661 cagccatcgc gttgctcttt tccattatcc gtgtctgtcc tgttccgttt tgtatcttag
   117721 atgaggtaga agcagcgtta gatgaagcca acgtgaaacg ttttggtcgt tacttgagcg
   117781 atttccaaga tgacacacaa tttatcgtag tcacacaccg caaaggaacg atggtcgcag
   117841 cagatgtact gtacggagtg acgatgcagg aatccggtgt atcaaaaatc gtttctgtac
   117901 ggatggaaga tatcaatgaa gaggcgaaaa tcaacgaaac gaaagtgtag gaacgaaaat
   117961 gatcaaatta gtagcaatcg atttggacgg tacattatta aatgaacaaa agctgatttc
   118021 agatgagaat aaacaagcat tagcacaagc aaaacagcaa ggggtcaaga tcgttctatg
   118081 tacaggacgc cccttagctg cgatggcgca ttatctgcaa gaattaggac tggtagatga
   118141 aggtgactac agcatcactt tcaacggcgg tttggtacaa aaaaatgata ctggtgaaat
   118201 catggaaaag aaagtgatgg aagtatcaga tatccaacgc ttatacaaac tggctcagca
   118261 attggatctg ccactagatg ttttgtctga caatgtggtt ttacagctgc ctagtgcccc
   118321 aaggaagcaa tccttgtaca atgtgttgaa taagctgctg caattccaac cagcggatat
   118381 aagcgatatt acagatgaat ttgtgcttaa taaggcagta atcgcttatg accaagaaga
   118441 attagatcct aaaatcaaag aaataccagc agaattccat gaaatgtatg agatcatcaa
   118501 aacaagaagt atgcttttgg aatttatgcc aaaaggtgtt acaaaagctt atggtatttc
   118561 tttattggca aaagatcttg gtttgaaaca agaagaaatc atggcgatcg gtgatgagga
   118621 aaatgattta ccgatgatcc aatatgctgg actaggcgta gcgatggaga atgctgtgcc
   118681 tttcgtcaaa gaagcagcag atttcgtgac agccagcaat gaagaaaatg gcgtagccaa
   118741 agtgatccaa aaatttgtat tagagtaggg ggagtcaaat gggatttttt gataaaatca
   118801 agcgtgcttt tactggcgaa gagaaaacag aagaagttga agtacaggag aagtacgaca
   118861 agggtttaga aaagacgcgt aaaacatttg gtgaacggat gaatgagttg tttgccaatt
   118921 ttcgaacagt cgatgaagac ttttttgaag aattagaaga aacacttatc ggtgcagatg
   118981 taggttttga aacagcgata cgaatcaccg aagcattacg tcaagaagtg aaactcagaa
   119041 atgcgaaaaa atcggcagaa gtccaaaatg cgatcatcga gaaacttgtg gatctctatg
   119101 aagcggaagg aatcaatgaa gtaaatgaac taaatatcca agaagacgga ctaacagtta
   119161 ttttgtttgt cggtgttaat ggtgtaggga aaacaacaag tattggtaaa cttgcccatc
   119221 agttcaaaaa cgaaggtaaa aaagtattga tggcagcagc agataccttc cgcgctggag
   119281 caatcgacca gttagttgtc tggggcgaac gcgctggtgt tgaagttgtt cgtggcaatg
   119341 caggaggcga tccagcagct gtcgtattcg atgctttgga aagagcaaag acagagcagg
   119401 cagatgtttt actagttgat actgcaggcc gactgcaaaa taaagtgaac ttgatgaacg
   119461 aattagaaaa aataaaacga gtaatccagc gggaattacc agaagcaccc caagaaacac
   119521 ttcttgtatt agatgcaacc actggtcaaa acgcgatggt tcaagcaaaa caattcaaag
   119581 aaacaacaga tgtgaccggt cttgttttga caaaattaga tggtacagca aagggcggta
   119641 tcgtacttgc tatccgtaat gaattgcatt taccagtgaa attggtcggt cttggtgaag
   119701 gaatcgatga cttggagccg tttgatccaa atgattttgt cgttggattg ttcaaaggat
   119761 tactaaaaga agaataaccc atgtctaaaa agcaggagct gtgacaaaag ttgcagctcc
   119821 ttgtttgaat gattgttttt aacatagctg tgtttttgat gccttcattt ttatacttat
   119881 ttgtaagcgg ttctcaaaca atttcgttct ttttcttaga ctttttgatt ataattagat
   119941 tcatatgaat tggaggtcac ttaatgatga attttaagca attattattg catgtcaatg
   120001 cgcgcccttt tatcgaccaa gctcgatttg gtatagaacg ggaaggacag cgggttgatc
   120061 ttgcaggaaa tctagcaaaa accgatcatc cagcaatctt tggcgatcga tcctatcacc
   120121 cctatatcca aacagatttt agcgaaacac aaacagagat gatcacccct gttaccgatt
   120181 ctattcccga attatttcag tatctcgctg ctgtttatga tgtgactgct cgttctatac
   120241 cgaaagaaga gatgatctgg ccattaagta tgccacctgc cttaccggaa aaagacgaag
   120301 agatcattat tgcaaaatta aaaaatttcg aagatgtctt gtatcgacgt tatttagcaa
   120361 aagaatacgg gaagcgaaaa cagatggtaa gcggtattca tttcaatttt gaatttggcg
   120421 atgagttgct aagaacattg ttcagccatc aggaagaatt tcaagatttt tccgaattca
   120481 aaacagaact ttatttgaaa acagcgagaa actttatgcg ctatcgctgg atgatcactt
   120541 atttattcgg tgcttcacca atgagtgaga agaactactt tttagatgaa tcacatccgc
   120601 aagaacctgt tcgcagtatt cgcaacagtg cgctgggcta tacgaatcat ccaaatgtga
   120661 aagtttccta tgcttctatg aaacagtatt tagcagatat cgagcgaatg atcgaagaag
   120721 ggaaactttc ggaggagaaa gaattttata caccgcttcg tttccgaggc ggaaagaaag
   120781 tcgcagattt agcaacaaca ggtgttcgtt atattgaatt gcggaatatc gatttgaatc
   120841 cttatgcaag attgggtatc aatccagagc aagttcggtt cttacaatta ttcctgatgt
   120901 acatgttatg gacagaagaa aaagaagact gtgaccaatg ggtagcagaa ggtacgactc
   120961 gaaataacaa agtagcacta gaacaaccat cagatcaaac agaatttcat caagaaggta
   121021 gagaaattct tgaaggaatg aagcaaatgc tggttgaatt ggattggttg gattctcttt
   121081 atttagtaga ggaagcattg actcaaatgg atcatcctga acaaacgtta gcagcgaaac
   121141 tctatcaaga agcacagcta tcgagtcagc aagaagttgc tgtggcattg ggacatcaat
   121201 attataaaga aagccatgaa cgtccctatc agttggccgg ttttcgcgaa atggaacttt
   121261 ctactcagat atttatgttc gatgccatcc aaaaaggtgt ccaagtcaaa gtattagatg
   121321 agtcggatca atttttgcgt ctgcaattcc aagaccatgt agaatatgtg aaaaatgcaa
   121381 acatgacaag taaggacagc tatatcgtcc cacttatcat ggaaaacaaa acagtaacga
   121441 aaaaagtctt aaaagaagct gggttccggg taccaggagg cgcagaattt tcatccatgg
   121501 aagaagcggt aaaagcttat ccgagatttg ccgagcaggc atttgtgatc aaacctaaat
   121561 caaccaatta cgggttaggt atcacgattt tcaaggaagg agctagtctg gaagattatc
   121621 aagcaggttt agccattgct tttcgtgaag acagttcggt tttagtagaa gaatttatgc
   121681 cgggaacgga atatcgtttc tttgtgatcg acggagaagt ccaagccatc atgttgcgag
   121741 tccctgctaa tgttattggt gacagtatcc gaacagtgaa agaacttgtt gaagaaaaaa
   121801 atagtgaccc tttgcgtgga acgaatcatc gtgcaccttt agaattgatc caattaggtg
   121861 aattagaaca attgatgttg aaagaacaag ggttgacgat cgaatcagtt ccccaagcca
   121921 atcaaattgt ttatctaagg gagaactcaa atatcagtac aggtggtgat tcgatcgata
   121981 tgactgatga attttctgaa gcttacaaaa aaatcgccgt ttctgcggta gaagcactag
   122041 gagctaagat tagtggtatc gatttgatta taccagataa agaaatcgat cctacaactg
   122101 ataaaaaagc atatgggatc atcgaagcaa atttcaatcc agccatgcat atgcatgttt
   122161 atccttttgc aggaaaaggc agaagattga cgatgaatgt attgaaattg ttatatcctg
   122221 aagtatttta aaagaaaaag cttttgacag attgtcctat ttgatgtcaa tctgatcaaa
   122281 agcttttttt gcatgtaaaa atttatgtgt tttttcgttt taaaataaac gatcccagaa
   122341 gctttttttc ttttttggtt tttctttttc agctggcgaa gagttttctt ctgggtattt
   122401 tttttcgata tcaatcacag gttcatttac tgcttctttt gctgttaaga gcaatccgaa
   122461 ttcctcagga tttgcagtga cgtaatcgtt gactacagtg aaacggctat tgtttttcgt
   122521 tgctagctgg atatattggt tttggacaga aaggggtaga gccccattca agagtatgga
   122581 agcatctgga tgttttttga gttcttttaa aaagttttct ttgttttttg gttggatcat
   122641 ttcagcaaca gtcatactaa gatagcagcg ttccctgaat gtccctaaat atttatgctg
   122701 ttcttcaggg ttcactaaag gtgtaccata aagtccctta tccaaatggc tttttacatc
   122761 atcttgtgcc ataaaaaata ccctccttct tctctttgtc tttcttacga ttatagcata
   122821 ttcccaataa tacctaaaac atatgaaaaa gcggttgctt ttgttcaacg attcacttaa
   122881 aataagaatg aaaatcattc tcaattagaa aggtggattg gatgcgctac ggacgactag
   122941 tagcattatt agttatttta agtagtattt cattgttcat cggagtatcg aatgtacaat
   123001 tgtcagaact gctccagtta gatgggcaaa gcttacaaat cttgctagtg actcgtttgc
   123061 cacgaacgat ttgtttgatc cttgtgggag cgaccagtag tatttgtggg ttgatcatgc
   123121 aacatttgac ccaaaataaa tttgtttcac caacgactgc gggcacgatg gatagtgcgc
   123181 gtttaggtat tttagtggct atgattttcc tacctggtgc atccttgatt gtccgatcct
   123241 tgacagcatt ttgctttgct tttgcaggaa cattgctttt tttatccctt acgcgattgt
   123301 tcctccagaa aaatcaattg atgcttccgt tgattggagt catgttaggg aatatcattg
   123361 gttcagttgc tacttttttt gcttatcaat tccagttggt acaaaatatg acttcatggc
   123421 ttcaagggaa ttttgcgact gtgatacgag gaaattatga gttattgtat gctgttgttc
   123481 cattgttgct cttggtttgg ctatttgctt accgatttac gatcattggt ctgggcgaat
   123541 cgtttgcagt aaatctggga atagattacc agaagatgca gtttttaggc attggcttag
   123601 tatcattggc tagtgcagtc atgttgatca tggtaggaag cattcctttt atcggagtga
   123661 tcattccgaa tcttgtctct agaatatatg gagatcaagt gcacaaaaca attggcgtaa
   123721 ctgctttagc aggtagtttg ttcttagtat tttgcgatct tgtcgctcgc gtgttgattt
   123781 ttccttatga aataccagtc agtgtgatcg ttggaatcat cggtggggct atcttccttt
   123841 atcttctgat aaggggacga cgatatgctt aaaaaaaagc catctatttt tattggtatg
   123901 cttgttcttg ttttatttgt tacaggaatg tacttgacga tccatacata tggaaactgg
   123961 cattttgcat gtgcattgag gagtaagaaa atttttgcat ttatccttgt ctcgctagct
   124021 gtcagcagtg ccacaatcag ctttcagaca attacgcata accagttttt gactccttca
   124081 gtattgggac ttgagtcgtt atatgtcctt gtccaaacgg tccttttctt ctttatcgga
   124141 ggagtgacga tgcttcagca gacaggtaca gtttttttct tattgaacgt tgggctgatg
   124201 gtcgggctga gtctattgtt atttttcttt cttttgaaaa aaatgagagc gaatcttttt
   124261 ttattactga tgtcagggat gattttaggt acgctttttg gtagtgtcag taccttttta
   124321 caagtcatca tggatccgaa tgaatatgat ttgcttcaag gcagattatt tgcaagcttt
   124381 ggcaatatcc agagtcagta tcttttgcct gcttctttga tcattatcgg ctgctggtac
   124441 ctgttatttc gatatcgaca tgtgctagac gttttccatc tcggaaatga acaagcagta
   124501 aacctcggga tagacacaga tcgttttgaa tttttgattt tatgttgtgt cagtcttttg
   124561 acagggacat caacggctct tgtgggtcca gttacttttt tagggtttat tgtcgcaaat
   124621 atcagttatc gtgtcttttc tacttatcgt catggtcagc tatttatcgg aagctttctg
   124681 gtagctatgc tgtttctgac aggaggacag ctcgcagtag agcagatttt cggctggaat
   124741 acaacagtga gtgtcgtcgt tgaatttgtc ggaggaattt attttgtcac aaaattgatt
   124801 cgcgaaagga agagtatcct atgattgatt tacaaaaggt caataaactg taccatggaa
   124861 aatcagtggt taaagatgta cagttaacga ttcctgaaaa gaagttgact gcttttatcg
   124921 gtccaaatgg tgctggaaag tcaacggtgt tgtcgatgat cagtcgtttg ataccaaaag
   124981 atacaggtga aatctattta gaccataacg aagtaaaggc ttggaaatcc aatgaattag
   125041 ccaaaaaatt atcgatccta cgacaatcga atgaaataaa tgtccaaatc accgtgaaag
   125101 aattagtaga atttggacga tttccttata cgaaaggaaa attgacgaca aaagacgagg
   125161 aaatcgttca ggaatcactg gaacacattg ggttagaaga aatagcggat caatcgattg
   125221 acactctttc aggcggtcaa ctccaacgtg cgtatattgc aatggtgcta gcgcaagaca
   125281 cggattatat tttactggat gaacctttga acaatctaga tatgaatttt gcggttcaaa
   125341 tcatgcagat tttaaaaaat ttagtacaag aaatcgggaa aacaattgtt attgttcttc
   125401 atgatatcaa ctttgctgca agttatgcag acgaaattgt tgcaatgaaa gatggacagt
   125461 tatttacgca aggaacgacc caagaaatga taaaaaaaga tgtattggat gaattatacg
   125521 aaatggacat acggatttgt gagattgaag gccgcagatt ctgtctttat ttctaaaact
   125581 aacattagga gagaagagat gaaaaaatta gtagtaggta tcttgattct agcatcattt
   125641 ggtttagcag catgtggaaa tagcaacaac accagtcagg cagacacaaa aaaaagcagc
   125701 acacaaactg aaactacatt gacgataaca gatagtaatg gagaccaaat cgaggtgcca
   125761 aataatccag aaaaagtcgt cgtattcgac aatggttctt tagatacgat ggatgctctt
   125821 ggtgttggag ataaggtagt tggagcagct acaagcagcc ttcctgagta tctttcatcc
   125881 tataaaaaag tagaatcagc aggtggaata aaagaaccag acttagaaaa aattaatcag
   125941 cttcagccag atctaatcat catttctggc cgacaaagag attttcaaaa agatctaagt
   126001 caaattgctc cgaccatttt cctttcttta gatgcgaaaa atccatggga atctttccag
   126061 caaaatgtca ctgctttagg ggagatcttt ggtaagcaag aagaagctaa aactcaatta
   126121 gaggaattat ctagtgcaat cgatcagacg aaaaagaaag cagaagctac tgacaaaaaa
   126181 gcactagtca cacttgtgaa tgaagggcaa ttatcagcct acggatctgg ttctagattc
   126241 ggtttcatcc atgatttatt tggctttgaa caagcagatg atcaaatcga agcatccact
   126301 cacggtcaaa gtgtttctta tgaatatgtg ctggaaaaaa atccggatat cttatttgta
   126361 gtagacagaa caaaagctat tggtggagac gacagcaaag atgatatttc tgccaatgaa
   126421 ctggtagcac aaacgaatgc tggtaaaaat cagcagatca tctcccttga accagatgtc
   126481 tggtatttaa gcgggggcgg tttagaatca atgaaactga tgatcgaaga tgtgaatcaa
   126541 gcatttaaat agcataaata acagaggatt aaactctcat actatgtaac aataatgttt
   126601 cttacattct taaatagcaa gagcctatcc tcaaagaagt tatcaaaaac catccgtaaa
   126661 atcgtattga tgattttacg gatggttttt atttatttga gaaatctttt ttcaacgtga
   126721 attcattatc tacatcatac gggttgattc tagaatgggg agtggcaaaa aatagtgtga
   126781 tccatttttt cagcatcaag atcgcctcct ttgatttgaa tcaaaggtac aaagcgaaac
   126841 ttagataaaa cttaaattta gatattttta ttttgtaccg atctcttctt taaacgatgt
   126901 tccatctgct gcttctcggc aataagtcca ctagactaga aaatatgaga agctattttc
   126961 tgtccagcgt tcttattact caaaacaaat cgcagttgtc acaatctctt ctaaacgatg
   127021 ttccatctgc tgcttctcgg caataagccc actagactag aaaatatgag aagctatttt
   127081 ctgtccagcg ttcttattac tcaaagcaaa tcgcagttgt cacaatctct tctaaacaat
   127141 gttcaatcag ctgaccttcg gtattagctc aaagcactga tttcacaacc tctatttagg
   127201 catgtgtctc agcaagcggt tgatagttga tgatctgcat ttttcgataa aaatcggcgg
   127261 gaagcttact ccaccgtttt agtaattgat agaaataact ctcttctgct ttgattttat
   127321 actcgcttaa tatcaatcca aaactattcg ttttttttga agctaatttt ttataaatat
   127381 ccaatgcttt ggttttatct tcattgacaa agagtgtttt tacttcaata taagaaccct
   127441 cgatattttg tgtttcttcg ataaaataga tgttttttat atattccttc atagtgattc
   127501 ctccaaagaa gtcttttatt tgataagttt actctaccga agagttgtta ataatccgtg
   127561 aacaaaaagt aattcttttt ccaatagttg ttaaaggttt tttatttttt atagtatttt
   127621 ttgttattta agaaaggaat aaggttaaac aaataacagg ttgctttttg ggtctttttc
   127681 cgttcaagaa actgaacgaa gaaaagggaa agttgtctct tttgtgataa gagaaatcat
   127741 aataaacgaa tgacttactt tccaataaat tgagaacagt cagaaccaga caaattagtt
   127801 gcatctgatg aataaagtga ttattctatg acatgaggtg atacagatga aattagaagt
   127861 gacaaaagaa gcacaagaag tattgaagaa taaattagaa gcagacgatc aacttttatt
   127921 agatatcgaa aacggtgacg gtccatttgc agaaagccaa gtcagttgcc aattggatac
   127981 ggcatttcga ttgatcattg tcaaaaaaga tacacaaaca gatctaagtc tttattctgt
   128041 agtggctgat acaccagttg gaccaatcaa aatcaaagac agtgcaatac tttacatgga
   128101 tgatccatcc acacttgctt tagaaccaac ttataatagt ttacaattaa aaggaccaag
   128161 cggcttgaga aaaggcaact tgcaagttgt tcgaaaagac tagagggata aaatgggaaa
   128221 attggcgatc atcagcgacc tacatgtgga catcaataaa ttcggcgaat cagagctgat
   128281 gcagctttgg caagttttgc aaaagcacaa gatcactcgg cttcatctag ccggagatac
   128341 ggctaataaa gtagaccgtt gcctagctgt tgtcgaattt ttcgtcaata aaggattatc
   128401 tacgacattt catttcggca atcacgagtt ggcagatgtg cccaatgaac agatgatgga
   128461 ccattttcca gacccacatt ttttaaatga acggtatatt gctttaaatg agcagaccgt
   128521 tcttttaggg atgaatggct ggtacgacta tcagttttcc gaattgaaag attacgaggc
   128581 aatcgtacga atgaagcgtt tgtattggta tgatcggttc atccagcgtg ggaaaacaga
   128641 tctcgaggtc aatgcagaaa tgcttgcagc tgcaaaaaat ttgctagacc agctggaagc
   128701 aaaaaaactt gaggtgattt tatccactca tttcgttcct aaacgggcat ttatcatgta
   128761 tcaatctgct ccttatgagc gttggaacaa attaaatgca tttttaggat cagaatcatt
   128821 cggagaatta cttgaccgtt attcaaatat caaacaagta gtttttggcc atacgcatcg
   128881 tcgctttgaa gataaatcca tcggtcacac gatttacagc tgcagacctt ttggttatta
   128941 ttatgaatgg cagctgaccc gagattttgt cttgaaagaa cagttgctag acagctatga
   129001 tcctatgaaa ttacgtgtcc tcttgcgtgc ccatcaccca gcttttcagg cctatcaaac
   129061 acaacatttg caagaggaat ttgaacaagc gatgacaatc gttccgtatt gagacagcat
   129121 tagtaatgtg taaaagaaaa taccttgtaa aacataagga taagtaagag cagtagatcc
   129181 atttatcgtg gatatgctgc tttttttgcg gaaaataatc agcgaatgtt tatttgatcg
   129241 gatgatttgt aactcgcaga gtactagatg caagcgtgta ttcttttttt gaacgataat
   129301 cacaaaaaaa aatataatat acatttttta tttaattata attaatattg attgaagtct
   129361 aggcagtcaa gtcgagagtg taaaatattt tgtgtaaatg aaaaaatcca tacaaaaaag
   129421 gaagtc
//
LOCUS       DACDIE010000002       125496 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.002, whole genome shotgun sequence.
ACCESSION   DACDIE010000002 DACDIE010000000
VERSION     DACDIE010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 125496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 125496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..125496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.002"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="IV250_000130"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="IV250_000130"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAP8767448.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="IV250_000131"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="IV250_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAP8767449.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="IV250_000132"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="IV250_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAP8767450.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="hflB"
                     /locus_tag="IV250_000133"
     CDS             complement(3742..5853)
                     /gene="hflB"
                     /locus_tag="IV250_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAP8767451.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="IV250_000134"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="IV250_000134"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAP8767452.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="IV250_000135"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="IV250_000135"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAP8767453.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="IV250_000136"
     CDS             complement(8053..8523)
                     /locus_tag="IV250_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HAP8767454.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="IV250_000137"
     CDS             complement(8569..8994)
                     /locus_tag="IV250_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HAP8767455.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="IV250_000138"
     CDS             complement(9061..9330)
                     /locus_tag="IV250_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAP8767456.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="IV250_000139"
     CDS             complement(9341..10936)
                     /locus_tag="IV250_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP8767457.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="IV250_000140"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="IV250_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAP8767458.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /locus_tag="IV250_000141"
     CDS             complement(14489..15049)
                     /locus_tag="IV250_000141"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAP8767459.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15402..16376
                     /locus_tag="IV250_000142"
     CDS             15402..16376
                     /locus_tag="IV250_000142"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP8767460.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16546..17946
                     /locus_tag="IV250_000143"
     CDS             16546..17946
                     /locus_tag="IV250_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAP8767461.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18024..18746)
                     /locus_tag="IV250_000144"
     CDS             complement(18024..18746)
                     /locus_tag="IV250_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP8767462.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18825..19331)
                     /locus_tag="IV250_000145"
     CDS             complement(18825..19331)
                     /locus_tag="IV250_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP8767463.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19454..21253
                     /locus_tag="IV250_000146"
     CDS             19454..21253
                     /locus_tag="IV250_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAP8767464.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21321..22895)
                     /locus_tag="IV250_000147"
     CDS             complement(21321..22895)
                     /locus_tag="IV250_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8767465.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="IV250_000148"
     CDS             complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="IV250_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAP8767466.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="IV250_000149"
     CDS             complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="IV250_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAP8767467.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24672..25310)
                     /locus_tag="IV250_000150"
     CDS             complement(24672..25310)
                     /locus_tag="IV250_000150"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAP8767468.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25311..25961)
                     /locus_tag="IV250_000151"
     CDS             complement(25311..25961)
                     /locus_tag="IV250_000151"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAP8767469.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="IV250_000152"
     CDS             complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="IV250_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAP8767470.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="IV250_000153"
     CDS             complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="IV250_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAP8767471.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29104..29844)
                     /locus_tag="IV250_000154"
     CDS             complement(29104..29844)
                     /locus_tag="IV250_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAP8767472.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="IV250_000155"
     CDS             complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="IV250_000155"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAP8767473.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31215..32153)
                     /locus_tag="IV250_000156"
     CDS             complement(31215..32153)
                     /locus_tag="IV250_000156"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAP8767474.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32146..32637)
                     /gene="def"
                     /locus_tag="IV250_000157"
     CDS             complement(32146..32637)
                     /gene="def"
                     /locus_tag="IV250_000157"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP8767475.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32652..35060)
                     /gene="priA"
                     /locus_tag="IV250_000158"
     CDS             complement(32652..35060)
                     /gene="priA"
                     /locus_tag="IV250_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAP8767476.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35313..35618)
                     /locus_tag="IV250_000159"
     CDS             complement(35313..35618)
                     /locus_tag="IV250_000159"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP8767477.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="IV250_000160"
     CDS             complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="IV250_000160"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAP8767478.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(36724..37008)
                     /locus_tag="IV250_000161"
     CDS             complement(36724..37008)
                     /locus_tag="IV250_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8767479.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(37026..37916)
                     /locus_tag="IV250_000162"
     CDS             complement(37026..37916)
                     /locus_tag="IV250_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAP8767480.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(37936..38154)
                     /locus_tag="IV250_000163"
     CDS             complement(37936..38154)
                     /locus_tag="IV250_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8767481.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(38124..39824)
                     /locus_tag="IV250_000164"
     CDS             complement(38124..39824)
                     /locus_tag="IV250_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAP8767482.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            40091..40864
                     /locus_tag="IV250_000165"
     CDS             40091..40864
                     /locus_tag="IV250_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8767483.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(40945..41514)
                     /gene="thiT"
                     /locus_tag="IV250_000166"
     CDS             complement(40945..41514)
                     /gene="thiT"
                     /locus_tag="IV250_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HAP8767484.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(41580..41682)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(41688..42659)
                     /locus_tag="IV250_000167"
     CDS             complement(41688..42659)
                     /locus_tag="IV250_000167"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP8767485.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(42753..44126)
                     /gene="glmU"
                     /locus_tag="IV250_000168"
     CDS             complement(42753..44126)
                     /gene="glmU"
                     /locus_tag="IV250_000168"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAP8767486.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(44629..45447)
                     /gene="purR"
                     /locus_tag="IV250_000169"
     CDS             complement(44629..45447)
                     /gene="purR"
                     /locus_tag="IV250_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAP8767487.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(45614..46438)
                     /locus_tag="IV250_000170"
     CDS             complement(45614..46438)
                     /locus_tag="IV250_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP8767488.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(46411..47091)
                     /locus_tag="IV250_000171"
     CDS             complement(46411..47091)
                     /locus_tag="IV250_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP8767489.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(47114..48052)
                     /locus_tag="IV250_000172"
     CDS             complement(47114..48052)
                     /locus_tag="IV250_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HAP8767490.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(48297..49148)
                     /locus_tag="IV250_000173"
     CDS             complement(48297..49148)
                     /locus_tag="IV250_000173"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAP8767491.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(49302..51875)
                     /gene="mprF"
                     /locus_tag="IV250_000174"
     CDS             complement(49302..51875)
                     /gene="mprF"
                     /locus_tag="IV250_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP8767492.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            52016..52822
                     /gene="proC"
                     /locus_tag="IV250_000175"
     CDS             52016..52822
                     /gene="proC"
                     /locus_tag="IV250_000175"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAP8767493.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(52901..54457)
                     /gene="rny"
                     /locus_tag="IV250_000176"
     CDS             complement(52901..54457)
                     /gene="rny"
                     /locus_tag="IV250_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAP8767494.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(54868..55917)
                     /gene="recA"
                     /locus_tag="IV250_000177"
     CDS             complement(54868..55917)
                     /gene="recA"
                     /locus_tag="IV250_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAP8767495.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(56026..57267)
                     /locus_tag="IV250_000178"
     CDS             complement(56026..57267)
                     /locus_tag="IV250_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAP8767496.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(57507..58085)
                     /gene="pgsA"
                     /locus_tag="IV250_000179"
     CDS             complement(57507..58085)
                     /gene="pgsA"
                     /locus_tag="IV250_000179"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAP8767497.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(58294..59178)
                     /locus_tag="IV250_000180"
     CDS             complement(58294..59178)
                     /locus_tag="IV250_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8767498.1"
                     /translation="MAFETIGEKLRQARLNKKISLDELQQITKIQKRYLEAIDNDDFD
                     QLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQPETVSTSRKAMHQ
                     EESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDTASSLKVEGSVEES
                     SSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITDATKPVTLEFTAVN
                     RVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNATIKANGESVPVNP
                     GENNQSPKNVNLTLQYAE"
     gene            complement(59956..61242)
                     /locus_tag="IV250_000181"
     CDS             complement(59956..61242)
                     /locus_tag="IV250_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP8767499.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(61235..62503)
                     /locus_tag="IV250_000182"
     CDS             complement(61235..62503)
                     /locus_tag="IV250_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP8767500.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            62755..63216
                     /gene="mscL"
                     /locus_tag="IV250_000183"
     CDS             62755..63216
                     /gene="mscL"
                     /locus_tag="IV250_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAP8767501.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(63275..63721)
                     /locus_tag="IV250_000184"
     CDS             complement(63275..63721)
                     /locus_tag="IV250_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8767502.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(63814..64059)
                     /locus_tag="IV250_000185"
     CDS             complement(63814..64059)
                     /locus_tag="IV250_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8767503.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(64174..64752)
                     /locus_tag="IV250_000186"
     CDS             complement(64174..64752)
                     /locus_tag="IV250_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP8767504.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(64829..65359)
                     /locus_tag="IV250_000187"
     CDS             complement(64829..65359)
                     /locus_tag="IV250_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAP8767505.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(65363..66223)
                     /gene="rlmB"
                     /locus_tag="IV250_000188"
     CDS             complement(65363..66223)
                     /gene="rlmB"
                     /locus_tag="IV250_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAP8767506.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(66216..66611)
                     /locus_tag="IV250_000189"
     CDS             complement(66216..66611)
                     /locus_tag="IV250_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP8767507.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(66608..68020)
                     /locus_tag="IV250_000190"
     CDS             complement(66608..68020)
                     /locus_tag="IV250_000190"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAP8767508.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(68017..68553)
                     /gene="cysE"
                     /locus_tag="IV250_000191"
     CDS             complement(68017..68553)
                     /gene="cysE"
                     /locus_tag="IV250_000191"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAP8767509.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            complement(68875..70335)
                     /locus_tag="IV250_000192"
     CDS             complement(68875..70335)
                     /locus_tag="IV250_000192"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAP8767510.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPKMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(70505..71662)
                     /locus_tag="IV250_000193"
     CDS             complement(70505..71662)
                     /locus_tag="IV250_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAP8767511.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(71696..73066)
                     /gene="radA"
                     /locus_tag="IV250_000194"
     CDS             complement(71696..73066)
                     /gene="radA"
                     /locus_tag="IV250_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAP8767512.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(73129..73656)
                     /locus_tag="IV250_000195"
     CDS             complement(73129..73656)
                     /locus_tag="IV250_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAP8767513.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            73886..74692
                     /gene="proB"
                     /locus_tag="IV250_000196"
     CDS             73886..74692
                     /gene="proB"
                     /locus_tag="IV250_000196"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAP8767514.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            74689..75936
                     /locus_tag="IV250_000197"
     CDS             74689..75936
                     /locus_tag="IV250_000197"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAP8767515.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            76012..76356
                     /locus_tag="IV250_000198"
     CDS             76012..76356
                     /locus_tag="IV250_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8767516.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQRERW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(76473..76799)
                     /locus_tag="IV250_000199"
     CDS             complement(76473..76799)
                     /locus_tag="IV250_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8767517.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(77414..78616)
                     /locus_tag="IV250_000200"
     CDS             complement(77414..78616)
                     /locus_tag="IV250_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8767518.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            78733..79248
                     /locus_tag="IV250_000201"
     CDS             78733..79248
                     /locus_tag="IV250_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP8767519.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(79447..79893)
                     /locus_tag="IV250_000202"
     CDS             complement(79447..79893)
                     /locus_tag="IV250_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP8767520.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(79940..81418)
                     /gene="msr"
                     /locus_tag="IV250_000203"
     CDS             complement(79940..81418)
                     /gene="msr"
                     /locus_tag="IV250_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP8767521.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(81772..82401)
                     /locus_tag="IV250_000204"
     CDS             complement(81772..82401)
                     /locus_tag="IV250_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP8767522.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(82385..82849)
                     /locus_tag="IV250_000205"
     CDS             complement(82385..82849)
                     /locus_tag="IV250_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8767523.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(83336..84604)
                     /locus_tag="IV250_000206"
     CDS             complement(83336..84604)
                     /locus_tag="IV250_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAP8767524.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(84835..85572)
                     /locus_tag="IV250_000207"
     CDS             complement(84835..85572)
                     /locus_tag="IV250_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8767525.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(85639..86207)
                     /locus_tag="IV250_000208"
                     /pseudo
     CDS             complement(85639..86207)
                     /locus_tag="IV250_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
     gene            86335..87207
                     /locus_tag="IV250_000209"
     CDS             86335..87207
                     /locus_tag="IV250_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8767526.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            87549..88421
                     /locus_tag="IV250_000210"
     CDS             87549..88421
                     /locus_tag="IV250_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8767527.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            88418..90376
                     /locus_tag="IV250_000211"
     CDS             88418..90376
                     /locus_tag="IV250_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8767528.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(90708..91559)
                     /locus_tag="IV250_000212"
     CDS             complement(90708..91559)
                     /locus_tag="IV250_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8767529.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            91682..92149
                     /locus_tag="IV250_000213"
     CDS             91682..92149
                     /locus_tag="IV250_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAP8767530.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(92412..92894)
                     /locus_tag="IV250_000214"
     CDS             complement(92412..92894)
                     /locus_tag="IV250_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP8767531.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(92994..93857)
                     /locus_tag="IV250_000215"
     CDS             complement(92994..93857)
                     /locus_tag="IV250_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP8767532.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            94455..95330
                     /locus_tag="IV250_000216"
     CDS             94455..95330
                     /locus_tag="IV250_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8767533.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(95475..95627)
                     /locus_tag="IV250_000217"
                     /pseudo
     CDS             complement(95475..95627)
                     /locus_tag="IV250_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(95895..97226)
                     /locus_tag="IV250_000218"
     CDS             complement(95895..97226)
                     /locus_tag="IV250_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8767534.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(97375..98088)
                     /locus_tag="IV250_000219"
     CDS             complement(97375..98088)
                     /locus_tag="IV250_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8767535.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            98263..99687
                     /locus_tag="IV250_000220"
     CDS             98263..99687
                     /locus_tag="IV250_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015508962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8767536.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            99986..100693
                     /locus_tag="IV250_000221"
     CDS             99986..100693
                     /locus_tag="IV250_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAP8767537.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            100680..101003
                     /locus_tag="IV250_000222"
     CDS             100680..101003
                     /locus_tag="IV250_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP8767538.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(101092..102129)
                     /locus_tag="IV250_000223"
     CDS             complement(101092..102129)
                     /locus_tag="IV250_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP8767539.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(102450..103433)
                     /locus_tag="IV250_000224"
     CDS             complement(102450..103433)
                     /locus_tag="IV250_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAP8767540.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(103848..104618)
                     /locus_tag="IV250_000225"
     CDS             complement(103848..104618)
                     /locus_tag="IV250_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8767541.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMIFGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(104611..105546)
                     /locus_tag="IV250_000226"
     CDS             complement(104611..105546)
                     /locus_tag="IV250_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8767542.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(105862..107511)
                     /locus_tag="IV250_000227"
     CDS             complement(105862..107511)
                     /locus_tag="IV250_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP8767543.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(107628..110231)
                     /locus_tag="IV250_000228"
     CDS             complement(107628..110231)
                     /locus_tag="IV250_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP8767544.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(110243..111292)
                     /locus_tag="IV250_000229"
     CDS             complement(110243..111292)
                     /locus_tag="IV250_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HAP8767545.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(111318..111632)
                     /locus_tag="IV250_000230"
     CDS             complement(111318..111632)
                     /locus_tag="IV250_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HAP8767546.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(111648..112094)
                     /locus_tag="IV250_000231"
     CDS             complement(111648..112094)
                     /locus_tag="IV250_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP8767547.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(112095..114071)
                     /locus_tag="IV250_000232"
     CDS             complement(112095..114071)
                     /locus_tag="IV250_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HAP8767548.1"
                     /translation="MKIEIIIVHLSCIQEVKIMGIEFSERQMKILYFLIENEHTTYKE
                     LSEQLYVSTKTISKDILILKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKIN
                     IADSPEDRLTYVFAKLLNTNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLV
                     SDRNGVRIKADEKSKRRLMSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDI
                     LKKVAELLNRYMNKTQLKFTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNT
                     LILIKMIEKAFSIIVPIDEQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVR
                     IKLNEFYPDEELINNLVLHLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEE
                     IEERFCIHFNEDEIAYVTLHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFA
                     DMVEIEDVLSINELQDRKVTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQL
                     NEQTEDAFLNLLDKRFCFFTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKL
                     SSTAMNDFAMPHIEVDFIKKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKI
                     YETFNEIASNHELLANLKTSSSFEKLVYILKEGL"
     gene            complement(114229..115125)
                     /locus_tag="IV250_000233"
     CDS             complement(114229..115125)
                     /locus_tag="IV250_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8767549.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(115118..115798)
                     /locus_tag="IV250_000234"
     CDS             complement(115118..115798)
                     /locus_tag="IV250_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8767550.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(115799..116581)
                     /locus_tag="IV250_000235"
     CDS             complement(115799..116581)
                     /locus_tag="IV250_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8767551.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(116574..117491)
                     /locus_tag="IV250_000236"
     CDS             complement(116574..117491)
                     /locus_tag="IV250_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8767552.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(117655..117978)
                     /locus_tag="IV250_000237"
     CDS             complement(117655..117978)
                     /locus_tag="IV250_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8767553.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
     gene            118766..121993
                     /gene="fss3"
                     /locus_tag="IV250_000238"
     CDS             118766..121993
                     /gene="fss3"
                     /locus_tag="IV250_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAP8767554.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYCQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            122106..122420
                     /locus_tag="IV250_000239"
     CDS             122106..122420
                     /locus_tag="IV250_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP8767555.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            122433..122807
                     /locus_tag="IV250_000240"
     CDS             122433..122807
                     /locus_tag="IV250_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP8767556.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            122817..123152
                     /locus_tag="IV250_000241"
     CDS             122817..123152
                     /locus_tag="IV250_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAP8767557.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            123235..124575
                     /locus_tag="IV250_000242"
     CDS             123235..124575
                     /locus_tag="IV250_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP8767558.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            124653..125327
                     /locus_tag="IV250_000243"
     CDS             124653..125327
                     /locus_tag="IV250_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8767559.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
BASE COUNT        36061 a        26370 c        22451 g        40614 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gttttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc tcctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatgggat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttatact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaagaa attaaaaatt tcttcaaagt ttaatgaaat
    15241 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15301 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15361 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15421 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    15481 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatc aatactgcta
    15541 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    15601 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    15661 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    15721 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    15781 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    15841 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    15901 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    15961 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16021 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16081 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16141 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16201 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16261 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16321 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16381 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16441 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    16501 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    16561 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    16621 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    16681 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    16741 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    16801 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    16861 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    16921 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    16981 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17041 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17101 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17161 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17221 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17281 gatattatga gttggctgac ctacaatgat ggaaaagtcg atttagatac agaacaagta
    17341 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17401 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    17461 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    17521 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    17581 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggcccttgaa
    17641 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    17701 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    17761 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    17821 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    17881 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    17941 ttctaacaaa aataatatat aaaaagaaag cacccgtacg attggtacag gtgctttctt
    18001 tttattatct aacaatgtgc tacttacatt tcagcagcag taaagacttc tgaaacatca
    18061 tcatcgtctt ctagcttatc tactagacgt tccaattgct ctttttgttc ctcatttaaa
    18121 tcaatcgtcg tttgtggaat catcgtcaat tctgcttgcg ccaaagtgaa ctctttttcc
    18181 aactcatctc gtacagcagc aaaatcttct ggtgcagtat aaatctcgaa tacttcagga
    18241 gaagtttgca gatcttctgc ccccgcctct aatacatcct caaacatcgt ctcttcatca
    18301 acagtcaacc catcgcgttc aatagcgata tatcctttgc ggtcaaacaa gtaactgact
    18361 gaacccgttt ctcccagtga tccaccgttt cgagtaaaag ctactcggac atttgtcgct
    18421 gtccgattac ggttatctgt caatgcatgg actaagattc ctactccacc cggaccatat
    18481 ccttcatacg tgatttcatc gtaattcgtt tcatctacgg cactcgtagc ctttttaatc
    18541 gcccgttcca cattgtcgtt aggcatattt gcagcttttg ctttgtccac aacaagccgc
    18601 aaagctggat tcatagatgg atcaggaccg cctgctttag ctgccatata aatttcacga
    18661 gaaagtttct gaaagacttt ccctcgtttt gcatcttggg cattttttct tccttggata
    18721 ttatgccatt tactatgtcc tgacatgatt tttcctccct tgttcttttg agaatacact
    18781 tctattctag tactatttca caaaaaaaca aagccacagc caatctataa acgcttcttg
    18841 aatgccgcaa aaagcacaca taatatcaca gcagtcagag caccgatcga atccaacatg
    18901 acatcttgga acataggtgt tcgtccaccg gtcatcatct gatggaattc gtccaaacct
    18961 gcgtaccctg ttgctgaaag ccaagcaagg atcgctgtca gccaccaaat ttttagcttg
    19021 gggaaaacac ctaaaaacaa gctgcctccc aaaataaaat aggtaccgaa atgtgcgccc
    19081 ttccttaaaa agaactctaa aaatttggcg tagcccagat ggttgatact caccatactt
    19141 ccaccataat taaaacgtat acccgctaat gtgtctttaa atggctgatt aggcagccag
    19201 ctttctaatc gcgaaacttg tgattgctgt ccgtaagttt gagaagaact gtaaaataag
    19261 attataacga ctatcagtgc gattactaaa taaatatttc cgtttttcat ttgttttttt
    19321 gaagaattca taagtcacct ctgtatttta tcttagactt tttttgaaga aacaacaatc
    19381 attttcatag agacttcttc cataaaaaag tgctatactt aattttgtat tacaatatga
    19441 agggagtttt ttcatgagtt attcattaaa ttggaatcta gattctattt tttccggagg
    19501 cagtcattct gatgctttga accaacggat gaagcaattg gaagaccaaa tgaacgagta
    19561 ttatcaccgc gtaacaaaat ggtcacctag ttctgacaat gcagagcagt taaacgctat
    19621 tcttcaattg caagaaacga ttacgaatgg ttttacccaa tgcagcagct atattactgc
    19681 attgctttct gccaatgtca atgattcaga tgcaaaggtc ttatcaggca agctttacgc
    19741 aatgctgcct cgtctgcaat cagccgaaac tgtattgtca aagaaatttg cagaaatatc
    19801 tgacaatgat tggaatcaga tgctttctca atcttcgttt gaaacaattg cttttcgttt
    19861 aaacgagatt cgtcgtgatg gctcacaatt attatcagaa gctgaagaaa acattataaa
    19921 tacgctttct ttagatggtc taaatgcttg gagcagtcat tatgatacga ttgtagccag
    19981 catttcgatt cccttcgaac aagatggaca agtagtcgaa ttatctgctg gacaagcctt
    20041 caataagatg atgggcgatc cagatccaaa agtcagagaa acacttttcg cagcatggga
    20101 agaagcctgg aaagaaaagg cacccctctt cacggatact ttgaatcatt tagatggttt
    20161 tagactttct gactatgaac ttcatggtgt cacagatttt ctacaaaaac cattagagta
    20221 caatcggttg aagaaagaaa cattgtctgt catgtgggat acaatccaga aaaacaaaca
    20281 accccttgtt aattacttga ctagaaaagc taatcttttc ggtaaagaaa aaatggaatg
    20341 gcaagatcaa gatgcaccta ttattttagg cgatttgaaa gaaaaaacct ttagttttga
    20401 cgaagcagca gcatttatta tcgaaaactt taacaaattc agtccaaaaa tggctaaatt
    20461 tgcccaatct gcttttgaaa agagctggat cgaagcagaa gatcgtcctg ggaaacgtcc
    20521 tggtggttat tgtactgagt taccagaaac aaaagaatca aggatcttta tgacctacag
    20581 taattctgtt aatgaggttg cgacacttgc tcatgaacta ggacatgctt tccatagcag
    20641 cacgatgtgg gatttacctt ctttaaatcg cgaatatgcc atgaatgtcg cagaaacagc
    20701 aagtacattc gcagaactga tcgtagccga tgctacatta aaagtcgcaa aaacaaaaga
    20761 agaaaaaatc aatttgctag atacaaaact gcaaaatgct ttagctatgt tcatgaatat
    20821 ccattcccga ttcatttttg aaaatcgatt ctatgaagca cggcaagaag gattagtctc
    20881 agaagataag atcacagaga tgatggttga ggcgcaaaaa gaaggctatc aaggagcttt
    20941 agctacttac catccatatt tctgggctgc aaaattgcat ttctttattg atgacgtacc
    21001 gttttataac ttcccataca catttggcta cctctttagt ttgggcatct atgcttacgc
    21061 caaccaaaag ggagcaaatt ttgaagaaga atatatccat ttactacgcg atactgcctc
    21121 aatgacgacg gaagatcttg cgaagaaaca tttgaatgtc gatcttaccc aaccagattt
    21181 ttggcaagca ggtatcgaca tggtgactga agatatccaa acgttcatgg aattgacaga
    21241 agaatttatc tgatttctat tttatcacaa taaaaaaaga gttagtccag aatgggctaa
    21301 ctctttttga ttaatcattc ttacatgctg acagcaaagt caggggcaaa ccattgaact
    21361 gaaccaactt ttacgctttc gcctggttga ggagcatgga tatattgtcc tccgcctaaa
    21421 gcaatcgcta cgtggtaagt tccgccttgt gacccccaga ataacaagtc tccagctttt
    21481 gcttgagaaa ctgaaatttt tgttccagct gattcttgag gaactgtcca accaccgatg
    21541 tcacgaccag ttacttgcat gtaaacatag cgtgtgaacc ctgagcagtc aaagccactt
    21601 ggatctttac cgccccaaac atatggagta ccaatatatt tgtaagcttc tgctacgatt
    21661 gctgcaccat tcacacttcc agaaggagct ggagcaggtg ttacaggttt tggttgttca
    21721 ggtgttggtg ttggcgttgg agccggagta ctacttcctg tgccgtttcc tgtatcaaca
    21781 ctttgatctg ttgatggtgt tgtaggcgca ggtgttgttg attcttctgt tgttgaagat
    21841 tcaggcactg ttgttgattc ttctgttgct gaagattcag gcactgttgt tgattcttct
    21901 gttgctgaag attcaggcgc tgttgttgat tcttctgttg ctgaagattc aggcgctgtt
    21961 gattcttctg ttgaagattc aggtgttgtt gattcttctg ttgctgaaga ttgtgtagct
    22021 gatgatgatt cttcagtcat tgaagattgt gtagctgatg attcttctgt tgctgagctt
    22081 tgtgcagtag atgaagcttg agtagcttgt gcttcagcct ctgcttgttc gcgtgcttct
    22141 ttttcagctt tttcttgtgc agcttgttga cgagcttgtt ctgctagacg ttgttgttca
    22201 cgaatacgag cttgctctgc ttcagcttct gctttttgac ggttcaagtc agctttcttg
    22261 tcttcagcag ttgcttgttc agcagctaat gaagttttca gtacgttcaa atcagcttgt
    22321 ttagataaaa gatcgccttt ttgagattcc aaagcagctt ggttttcagc taattctttt
    22381 agtttagcat cgttttcagc ttttttgtct tctacagctt ttttatcttg tttttgttgt
    22441 tccatcaagt cgttgtttgc ttttaccatc gttgtcattg cttgaacgcg tccaatagca
    22501 tctgctaatg aatcagcatt caatacagca tcgatgtagt ttgagcttgt gttgcttact
    22561 tgtgcttcgc gtgcttgttt ttggattgta tcttcacgtt tttcaatacg ttcttgtaaa
    22621 tctgcaatgt cttttactaa ttgtgcagat tcttgacgca atgtatcttg ttttgctaat
    22681 aggtcttgtg cttgtgtgtt gatttcagaa acttggcttt ctaaggcatc gatttgtgat
    22741 tgtgcatctg cttgttgatt tttcaagtct gcaatctttt gatcttgctg ttgtatctga
    22801 gaatcaaaat cgtccgccgc ggcagcgatc ggtgatgcta cggcagtcaa ggtcattgaa
    22861 cataccatta ctgctgatat taaactcttt ttcactcttc attcctccga ctggcttaat
    22921 ttaataaata tctaaatata tttaagtctt tcttaacgat ataaaaattg taccatagca
    22981 gtgtgtcaac gatataatag ttatgttaca aaaatgtttc aaaacaactt ttaaaaatga
    23041 caaaactgtt cgtttcagaa aatgatcatg aaaaagccgc gttaagtcta gcatttctta
    23101 agataacaac gattcttttt cgctttcgac ctaaaaataa aaaaagaaat tattcatcac
    23161 tgaataattt cttaaaggga aaaatgaata atacaaatat aatcatgttc aacagcagtg
    23221 taggtcctaa aaaccttgta ataaaatacg tgttatttac tactgctaat ttaaaaatca
    23281 actggaccac catcgtaaac aattcataac ccgtgacaaa gatgatcata ctgaaaaatt
    23341 cagtaaagat gttgacatgg ataacgtctt tcattacata catcaaccat acaattaaag
    23401 ggagagctac tgcatagata ccgataacgc caatatagta agcgtcatag atagccccga
    23461 gaacaatcgt ggtaatcagt aaataacgtt tttcaaacgc catactgcaa cataataatg
    23521 ccaatatcag aaagtgggca tttgacatat aggtgccttt cgaccactcc cctagcattc
    23581 ttgttaaatg accatctatc aacatcaaaa agaacaaaac gattggcaga tagtacttca
    23641 ttgtttcttt cttcaacatg gactaatcct ccgctagccg ttggacaatc gtcacaactg
    23701 acacgtcata catttctgca taaggtttca cataaacttc ccgatccagc ccataactat
    23761 ctggtttcgt tttaataacc gtaccgatcg gcaagttagc tggagaattc cctccaagac
    23821 cggatgtctg gacaacatcc ccttctttga tatccgtatc accagttaat tgggtcacca
    23881 ctaaggcatg gagcttttca tcatagtttt taagcaaacc aaacgcttcg ccattagccg
    23941 aagatacccg tactggaaaa tgattggagc tttcattaga ggatgacagt aattcgattt
    24001 tagacgaagc cgtattgacc tcgatcactc ggccaatcaa tcctttttgc gacatgactg
    24061 ccatatttgc ttcgataccg tctttagaac ccttgtctac gatcagcatg tcctgccaac
    24121 tgtcaggcga acgagtaatg acatttgccg ttactttttc gtaactagtc agcgtctgat
    24181 tcaactcaag ttcttttttc aacgcttcga tttctctctc gtaattcttg ttttgctgtg
    24241 aaacgtcatc atagctatcg attttttctt ttagccgctc attttcggca taagttgaaa
    24301 aaagattatg gacagaatct actccatttc gtacccaccg tactggggca gagattgttt
    24361 tatcaaccat tgcaacactg tcattaacga tggattggaa gaaattgggc tttccttcat
    24421 tcgctcgctg cgcagcggtc acacttagta tcgtcaccac gatgataaca ataaccaagg
    24481 taataataat atttttatta ggattgaact tcttcacaaa gacacctcga attaattaga
    24541 ctcttgtgtt caattttatc actaaatgcc gtgaaacaaa aggtaagttg ctcctcttaa
    24601 gctttttttt agcaaataat agattctgtc atttttagcc tgaaagacaa atttttttat
    24661 ttgtttatgg atcaatcttt gctttgaata actgcaatca taccatctga aaaagaaaat
    24721 tctgcggtat ctgaaagaaa ttcgttactt gcataagatg tcggatattc tacttcttta
    24781 ttttccaata catacttgct gccttttaat gtaagattcg agacgggagt caagcagcaa
    24841 taagctaaat acttcatatc tggtatctgt cttactatat gctctccagg caaataataa
    24901 gacaaataat tttgacaatc tctgataata agctgatggg caaagggttt gaagcgaggt
    24961 tccataccta accagagatt ggctaaaaga tgatccaatc ttccgcccgt agctccaatc
    25021 aaagtgactt ccgcttgcgg gtaacgttca aatgttttta agatacctaa ctgtgtatct
    25081 gtatcgtctt tttctgcagg ggaacgggta tactctttgg cttttgcaaa tacttccttc
    25141 tgctcttcgt tcgacaatga atcaaaatcg cccactgcca gatctactgg gtagcctgct
    25201 tgaagcaggt acagcgcacc acggtctatc ccgacatagg catcgtattt ggacagcaga
    25261 gactgcggcc agttttgcgg attacctcca gcagctaata aaattttcat ttagtctaat
    25321 gcctctttta atcgttcgat ttgtccgact ggattttcgg ctccatagat atatgatcct
    25381 gcaacgaaga catccgcccc tgcatcttta cagatttttg ctgtttctgg aacgattccg
    25441 ccatctactt cgattgcata agtccaattg ttctctctac gtagatcagc gagttccgta
    25501 attttttcta ctgtttcttc aataaagctt tgtccgccga acccaggatt gacagtcatg
    25561 actagtactt gatcagccaa aggcaaaaca tgtttgatca tggagactgg tgttcctgga
    25621 ttgataacta ctccagcttt aactcctaag ctttttgtca tttgaagtgc ccgatgaata
    25681 tgaggagttg cttcgacgtg gacagtaatg atatctgctc cagcttttgc aaatgctgga
    25741 atgtagtttt ctggatcaac gatcatcaga tggacgtcta acggtaattt agtaacagga
    25801 cggattgcct gaacaatatt tggccctaat gtgatatttg ggacaaattg gccatccatt
    25861 acatctacgt ggatgtaatc tgcgcctcct ttttccacta actcaatgtc tctttgtaaa
    25921 ttggcaaaat ctgcactcaa aatggatggt gctatcttca ttcttgtttc ctcctatttt
    25981 cgtttacctt ctttttttgc atacattggt tttctttttt cgatttcttg taagaattgc
    26041 aaataattgt cgtaacgtgt ctgtgcgata ccacctgttt ctactctatg ttttacttcg
    26101 cagccaggtt ctttagcatg catgcactct ctaaatttgc aatggacaga agcttctaca
    26161 aattcaggaa attgtctcgg cagttctcct gcttcaattg tcaaaaagtc aatcgagcta
    26221 aatcctggtg tatctgctac taacccgcca tagataggaa tcagttccac atgacgtgtt
    26281 gtatgccgtc ctcttccaag tgcatcagaa atctctcctg ttgctaaatt cagttccggt
    26341 gatatttgat tcagtagcgt cgattttcct gctccagact gacccataaa gaccgatagc
    26401 cgttctggga agtaacggat cagttctgtc gtatcacccg tatgattggg tacaatcacc
    26461 ggatagccta ttttagtata ggtttgcttg atctcttcga ttttttctgg gtcgtctgac
    26521 aagtcggtct ttgtcaaaaa aataatcggt tcgatcgact cgtattccaa ggtaactaga
    26581 aagcggtcaa gtagattata agaaaatgca ggctctacga tacttgtgac aatgattcca
    26641 agatcaacat ttgtgactgg tgggcgaact aattgatttt ttctcggcaa gatttccaga
    26701 agatagccgt ctgtttgatt ttcactctca aagatcacct gatctccgac aagcggcgtg
    26761 attttccgat tccggaaatt tcctcttcct cttgtttgat aggtctctcc gtttgcgtat
    26821 acatagtaaa aaccgctgat tgcttttctg atttgtcctt tgataagtgc cacctccgtt
    26881 tttgtttatt ttaccatacg aagaggaaag taggtgttat tccatgcaaa cttctcaaag
    26941 tatatggctt gaaaattgat caaaagagat agaaacacaa aaaagagaat gcaacaaatc
    27001 aatgtcgcat tcccttctga tatattttat tcagcactta ttttgaactt tctgaggtgc
    27061 tagctgatga acttgtggag ctggaagatt cttcttttga agagttgctt gtgctactgg
    27121 agctacttga gctgctagaa ctgtctgaag agtctttttc agaactagaa gacgctggat
    27181 ctggtccttt agagtaaatt acgttcacaa ccattccttt ggtaattgtc gttcctgccc
    27241 ctggtactgt acgaatcact ttatctttat ccaccgtgtt tgaatattcg gaagtctcat
    27301 gcccgatata ttctgcaccg acactagcta aataactttg tgcttcgctt tttgtatagc
    27361 cggagatatc attcaattta ggctctgttc cttggcttac gacaaaattg atgtaagttt
    27421 cttctgctac aacttcaccc gaggctggat cctgactgat gatcgaacct gatggtacag
    27481 tgtcgctata gtcataagta gcactaatat tattgatatt gaatccttgt gtaacaagcc
    27541 aattacgaac agtttcgtag ttttctccta catgactact caaagtcact gtttctggtc
    27601 ctttgctgac tgtgaaagta atcgtgtctg ttttaggatc gacttctgta tcttctgatg
    27661 gagattgcga aatgatcatt ccgctttcta cctctgaact gaattcctct tctttttgaa
    27721 tcaagtcagc agaaaaccct aattctttca aacttgcttc ggcatcttca taacttttac
    27781 cactgtaatc atctaatttg atttttttcg tgccgctact aatatacaga gtgacttcgc
    27841 gtttttgctt gactgtcgtt cccgctgccg ggtctgtctt cactacatta ccttcttcga
    27901 ttttgtcatc tgcaatttct tctgtttttg ttgctacttc taatttcgct tttgcgagtg
    27961 tttctctagc agaagcttca gaaagatcag aaacatcagg aacaacaact tctcctcgcc
    28021 cgccagaagc aaagaataca gctgttccga ttcctaaaac agcaagaata gctaatagga
    28081 tcagccacca ttttttctgt cctttctttt taggatcagg cagaacttct tcctcctctg
    28141 gtaaggtttc gttttctggc ttgtcttttg gcagaggcat agatttgaat gaatcaggca
    28201 ttggtgcatc ttccgtaagg ggtgtgataa cttttgtttc attatccatc acatgaggct
    28261 gccatttttc ttcgtttgca cgagctggtg ataatactgt agaaagatca tcagacattt
    28321 cctcggctga cttataacgg tctgcaggtt ctttagccgt tgcccgtaaa atcacgtttt
    28381 ccagtgcttg tggcacattt ggatcaagtg cttttataga tggaagatca tcttgaaaat
    28441 gttttaaagc aattgttaca gcagattcac catcaaacgg tacgctaccc gtcaacatct
    28501 cataaaggat gatccctaaa gcataaatat ctgactgctt cgttgccata cttcctctgg
    28561 cctgctcagg ggacagataa tgaacagatc ctagcatcgt attcgtctgc gtgatcgatg
    28621 tttcagataa agcgatagca atcccgaaat ccgtgatttt tacaacacct tcattatcga
    28681 tcagtacgtt ttggggtttc aaatctcggt ggatgatttg atggttatga gctaaagaaa
    28741 tagcagaaag gatctgctgc ataatattta ctgctgtttc ataaggaaca ggataatgtg
    28801 tttggatata tcgtttcaag tccattcctt tgacgtattc catcaccaga tactgcatgt
    28861 tgtcttcttc accaacatcg taaacactaa caatgtttgg atgaacgagt tctgttgctg
    28921 ctaaagcttc tctctgaaat cgtcgaatcg cagtctggtc attttgaaag tcaaatcgta
    28981 agactttaac tgctacatca cgatccaaga ttaaatcgtg cgccaaaaat acatttgcca
    29041 taccaccgct accgatattt ccagtgattt gataacggcc atttaatttt ttgcccaact
    29101 cgatcataca ctgtcctcct tatattcgat aagaagaacg gtgatattat ccgctccgcc
    29161 tgcttcattg gcacgatcaa tcagctcagt tactttatga gacaatgggc cttcttgatt
    29221 gatgatcgtt cctatttctt cttcagacag catgttcgtc aagccatctg aacataacag
    29281 cagtcgatct tttagttgcc aaagatgagt agaaacatct acttcgactg ttccaggcat
    29341 gccgacagaa cgtgttaaaa tatttttcct tggatggttc actgccattt cttcgctgat
    29401 ttctcctgat ttgactagtt cattgactaa agaatgatcc tccgtcaatt gcatgatttt
    29461 atcttcacga atcaaataag ctctgctatc cccaacattg gcaatcgtga accagtcatc
    29521 tgacagagcg gccgctacga ttgttgttcc cataccgtta tattccggct gttcttgtcc
    29581 acgctgataa atcagcatat tttcttcttg aatcgattga atgaaccatt gggcgatttt
    29641 ttcatcgtca acagcttctt gtttttccca agcttcgcct agattcgtta ccgccatctg
    29701 actggcaatg tctccggcac gatgaccacc catcccgtct gctaatacag caagcttgag
    29761 acctgaacgg ctggtaaata cactggcata gtcttgattt gtgttacgtc gtcttcctac
    29821 atctgattga tattcaatct gcattttttc acctcattac tttttgcgca aacaacaaat
    29881 gaaaaatcca tctgtgtaaa actgatgcgg atacaaagtc aacattttct cttcgattga
    29941 tgattgtagc ataggattgg tcaaaacatc aatcctctca aaatcagggt gtctttccaa
    30001 gaaagcagaa acaacgtctt ggttttcttc ttttaagatc gtacaagtgc tataagtcag
    30061 aataccggaa gactttaaag ttggtgcgca gctttctaaa atttccaatt ggatttttgg
    30121 taaattcgct aattcatccg gctgtttttt ataacgaata tctggttttc gccgaagcag
    30181 cccaagacct gaacaaggcg cgtctactaa aatccgatca aactgctctg cttcgaactg
    30241 tttactgact tctctagcat ccatcttttg agcgtagaca gcagaagaga cacctagacg
    30301 tgctgcatta tcttcaatga gtttgacttt gtgttcgtgt acatctaaag cgaccacttc
    30361 tccaccgcat gacggatcta aaaaagaagc aatatgcgtc gtcttacctc cgggtgctgc
    30421 acaggcatct aagacatgat gttcaggctc gatttgcata gacggcgcta caagcatcga
    30481 actttcatct tggatcgtca tgacgccatc gtggaacaat gagctagaag ctaaatgccc
    30541 ttttttcgct actataccgt atggactgac ttggcttttt tcagctgtga tctcttcttc
    30601 ttgcaaaaca gtgatggcct gatctctgga aagtcggcgt gtatctactc gggcactagc
    30661 atggctaggc tgatatagcg ataaaccgag cttttcagtt tcttcgtatc cgatttgatc
    30721 caccagtcgt ttcgtcatcc atagaggcag gctgattttg acagaaagtc tttcgatagg
    30781 atcagcaatg ccttgcagat ctggtgctcc ttgacgctga taattcctca agacaccatt
    30841 tacaaatttc cctgtcccag gatttccttt tacttttgcg atttcgaccg cctcgtttaa
    30901 aatagcatgt gcagggactt tatctaaaaa ttctaattgg tatacagaaa gataaagaag
    30961 catcttgacc cagtcatcca ctttttttgc tttagcgatc aatggtgcaa cgtaaaattc
    31021 caatagtcgc tgatggctaa tcgtaccata gatcaattct gtaaataaac ccacatcttt
    31081 ttgatttagc tcgctttgtt tgatcatctc attcaacaaa agattcgagt aggcgccacc
    31141 cttgttcact cgctctaatg tcagtaatgc catatatcgt actgattttt tcattttttt
    31201 agggattttt ttcattattt cacctgctga ccaatctgaa cttgctgacc gcttccgtta
    31261 aggaaatcat gtacggcctg ttttccttta ccagctggct gtaattcatt gatggctaag
    31321 acggtctgtt taccgcaagc aatccataga ttttttttgt ctttccggat gatcgtacca
    31381 ggtgctttgt ctgtagtttc tgccaatact tcaaccgata ataatttcca gcgtatgccc
    31441 tcataggtcg tgaatgcaat tggccaagga cgcattccgc gtacttggtt gtcaatttct
    31501 tctgcagttt tatgccaatc gattgcttct tgttcccgtg tgatgtttgg cgaaaaagtt
    31561 gcttctgact cgtcttgagg tactggtttt tgtccatcca aaatattggg caatgtttct
    31621 agcaatagct ttcttcctaa aaggctaagc ttttcaaaca tcgtccctac atcatcttgt
    31681 ttagtgattg gtatgctttc ttgcgcatag attccgcctg catccatttt tttgatcatt
    31741 tccatgatcg ttacaccagt ttctttctcc ccattcatga tggcataatg aacaggcgca
    31801 cctccccgat atttaggaag taaagaagca tggacattga ttgcccctaa ttttggtact
    31861 tgcaataact tttcaggcaa gaattggcca aatgccgcag tgacgatcac atcaggcacc
    31921 aattcaatga tttcttccat ttctggcgaa ccggagattt tctctggctg cagcacacgg
    31981 ataccgtgct tcagagccgc tttttttacc ggtgtcggcg taattactcg tttccgccca
    32041 actggacggt cgggctgtgt taccactgcc tggatctcgt aacctgattc tatcagccct
    32101 tcaagaatcg gcacagaaaa agcaggtgtg cccataaata ctagcttagt catcaagatg
    32161 ttcctccata tattcttcaa gttcttcttc cgggatctgc tgtatcattt tctcaatgaa
    32221 caagataccg tctaagtgat cgatttcatg ctggaaggca cgggctaaat agccaaaagc
    32281 cgtcacttct atctcttctc cgtctcgatc ataataacgc acggtcactt catctgctcg
    32341 cttgaccgtt ccatagacat gcggaatact gagacaacct tctacgtcaa ggctttcacc
    32401 ttttgcttcg atgatttctg ggttaatcag ttcaaatttc tcgccttcat ctacttcgat
    32461 cacagcaatt ctcttgtttt gtcctacttg aggagcagcg atccctattc cgtcattcgc
    32521 aatcattgtc tcatataaat tgtctaataa agcgattgtc tcgtccgtga ttacatcgat
    32581 tggctgagca gttcttttta atttatcatt agggtggatc aatataggat agcgcataat
    32641 ttggcccttt cttaaataaa gtttaatggt tcgttgtcaa ttgccacaaa aagaccttgt
    32701 cgctgttctt tctggcttcc attcaaaatt tcatccaaaa catgaggaag attcggttca
    32761 tgtttatatt tgatgattag ttgataataa tatcggtttt tcactctcaa aatcatgctg
    32821 ggcgttggcc ctaatagcaa gctttgaggg cttaacactt cttttagctg attggcaatc
    32881 tgaaagattt tctttgccgc tacttgttct tcaggatggc tggcggttat tttcacagta
    32941 aagtaataag gaggatagcc tccacgatgc cgtacatgca tttcctgctg ataaaactgc
    33001 tcataatttt gttttttagc caattctatg gcgtaatgtt gaggattgaa tgtttggatc
    33061 acgacttcgc ctgctttttc cgctcgccct gctcgtcctg aaacttgggt aagcaattgg
    33121 aatgtatgct cactagaacg aaaatccggt aaattcaggg aggtatcagc attcaacacc
    33181 ccaactaatg tcacttctgg aaagtccaaa cctttagcaa tcatttgtgt ccctaataat
    33241 atatctgctt cttttgcacc gaatttttgt aaaatctgtt cgtgtgcccc ttttcttcga
    33301 gtggtatcta catccatacg caaaatccta gcttgcggat aaagttctcg gagttcctct
    33361 tctacttttt gagtacctgt tccatagtag cggattttat tgccgccaca attaggacag
    33421 cgattgggaa taggttcttc atgtccgcaa taatggcagc gcattgaacg tgtatccata
    33481 tgcagtgtaa gagagatatc gcaattagga catggaagaa caaatccaca gtccctacac
    33541 atcacaaaag acgaatatcc tcgacgattg agcagtagga cagtttgttc ttttttgtct
    33601 aatcgatttt gtattttttc ctgcaaatta gcagaaaaag tagaggtccg atgattctga
    33661 aactcttcac gcatatctac gatctcaata gctggaagct gtgcagctgc tttggctcgg
    33721 tggtttaact gcaataactg ataaacgcct ttttgagctc gtgctcttga ttctaaagag
    33781 ggcgtcgcgc ttcctagaac tattgggcaa tgatgatatt tactgcgcca aattgctaga
    33841 tctctggcat gataacgcgg tgtctcatct tgtttgtaac ttgattcatg ttcttcatcg
    33901 ataatgatca gcccgatatt tttcagcggg gcaaaaatag cagatcgtgc gcctactact
    33961 acttgcgctt cttcccgctc aatcttgcgc cattcatcat atttttcacc ttgggaaagt
    34021 ccgctatgta agaccgcaac tgattctccg aagcgtcctt tgaatcgctc gaccatttgc
    34081 ggagtcaatg cgatttcagg cactaacata atagccgttt tattttctga caaaacatca
    34141 gcgattgcct gaagatatac ttctgttttc ccgctgccag tgattccctc cagcaaaaag
    34201 acctgatctt tttgctgctg acctgctgtg atgatttgtt cgacagcatt tttttgttct
    34261 gcattcagtt ccaatgccgt cgtttggtca aatactcggt ctttataagg gtcgcgataa
    34321 cgttctgatt ctataaactc cagccagccc ttttcttttc cttgattcaa gacagcagta
    34381 ctgattcctt tatttttgaa ataagcaatt ggttcttctt ggctcagttc agataaacac
    34441 aaaagaagtt ctttttgttt cttggcattt tgccgtaatt ttgcccattc ttcttcgatt
    34501 tgcttttcag ttaacaaaga acggaccaaa cgaatcgttt ttactttatt tcgtgtatgg
    34561 acttcatatt tgatatctac taattgctct tggcgccagc gaatcaattg agaaaggctg
    34621 ccttcttctt ctgcttcctt ccaggaaatc acttctcgct gatcaaacaa tacctgaact
    34681 tctggtgtgt ctgccaaagg atagatcaat ttatcatatt ctgctttcat cacactcgga
    34741 agcatcgttt gtagacaagt gattttgaat gcgtaagtaa tttttttcat ataatcagct
    34801 aatgaaagta attcttcatt gaccaccgga gcaaggtcta gcaaacgaat aactgccttc
    34861 aaagatggat tagctgattc ttctgatttt tggatagcca taacaaatcc ttggacatga
    34921 cgattgccat taccaaaagg gacttctact cgcatcccga cttgcaaagc tgtttccatt
    34981 tcagagggaa ccaaataagt gaatggctga tcggtctgca tagtaggtac gtccacaatg
    35041 acttcagctg ttaatgtcat ggttcgtttc ctcctttttt tgtcttttct tatcaagcga
    35101 caaaaagttc cttgcctatt ttactagaga tcgatggatt tgtcttatct tctgccaaat
    35161 cctaacaaaa aattaacagc agataagaaa aaaaagaact gtgtcaaaaa gttcagacac
    35221 agccttttct accagacaat aagtggcgaa tgtttgaaaa ggaaacagct gggtttgctt
    35281 ttctttttag atgttcgctc tatttttctt tattacattt tttgctcttc acggatacgt
    35341 gattccaatt cgtgttgttc acgctctttt tgtgcttgac gttcctcttc ttccatcttc
    35401 aaacgttcac gttttaattc aggatgtgga tcattgacta catttcctgc atcgatttct
    35461 tctaaagctt gaccgacgct ttttactgaa tcaaaagaat ctagagtagg ctgtgcactt
    35521 gcatccaatt catgcgcgcg tttacttgct aaaatcacta gagaatattt tgaatttaca
    35581 cgatctaata gtgagtcaat tgaaggtttt aacatcataa gactacatct cctttaacat
    35641 tttaatatat ttaccgatta cgcgatctac tcgaaaatgt tcactggcaa taatgttttt
    35701 gattcgttca actgctagcg gcacttcatc atttacaaca gcatagtcgt aaagagccat
    35761 catttcgatt tcttcttttg caactttcat tcgtttttcg attacttcat ctgcatctgt
    35821 tcctcggcct acgatccgtg attttaattc cgctaaatct ggcggagtca aaaagatgaa
    35881 tacaccatcg ggcacttttt ctttgacttg ttttgctcct tggacttcaa tctccaagaa
    35941 aacatctttt ccttcatcca atgtcttgtt gacatatgac aaaggagttc cataataatt
    36001 cccgacatat tctgcgtatt ccagcatctc tccagcttcg atcattgatt caaactcttc
    36061 tttgcttcgg aagaaataat caacgccttc gacctcccct tcacgcattt tacgggtagt
    36121 cattgagatc gaatactgaa aatcattatc ctcactctca aaaattgctt ttcgtactgt
    36181 gccttttcct acaccggatg gaccagacaa cacgattaat aatccacgct ctgacatgac
    36241 gacgtccttt caactagtct ttttgtactt atcgtttatt ttgcactttt tttctgcaac
    36301 tttcaaggtt atctttaatt atagctagaa attggtaaaa aagctaccct gtaccaagag
    36361 gtcgtaaaaa agctgtcctg catcaagtaa taagaacagc caaacataaa cagcttctca
    36421 tgtttttcgt ttggttgggg ttattatcgc agatgcaagc ttttgaacgc cgtgtataag
    36481 aggtcgtgaa gaagccgatt agtttcgagt atgaagaaaa attttgtgaa aatagtttct
    36541 cacatttttg cataaatttg gcttaatacc gagaaaatgg tttttgaaca tcacttattc
    36601 aaaataaaaa aagattgtga cacgaatttt gtcacaacct ttaaatgtat tcctagagca
    36661 aagaagctaa gcgaagaaat ccaattctgt ttatttattg tatttctttg ttcagcatcc
    36721 ttgctaaaag cccatattgg acaaagtatt cactacaccc atgatctcat cttttcgaga
    36781 acgtgctttt tctgtcggat cttctacctc ttctgtgtca attcgaacaa taaatttccg
    36841 attcatcgtc acgtaagcac cgtcgttgat caaaataaac agttcgttct cattatagtc
    36901 tgcacaggca tctttccata cgtcaaagcc gtttttttca gaagggatat cccgtttaac
    36961 cacagttttt ccacttaatt cgtggaacgt tacgtcataa tatttcatta tctctacctc
    37021 tttcgtcatt caatattttg tacttgttct ctgatttttt ctaagatcgt cttagactgg
    37081 acgacttgat ttttaatttc gattgcattt gatttggaac cgatcgtgtt gatttcccga
    37141 ttcatttctt ggatcaggaa atctaattcc cgacctacag gacattgtgc ttcgataagt
    37201 tcatctagtt tttgtacatg gatcgccaga cgatccagtt cctcgtgaat atctccgcgt
    37261 tccaacaaga tggctagctc cgtcaacaac cgttgctcat cgactttcgc tccaagaaat
    37321 tcctccagct ttttttgata ccttatttga aattcctttt catagatttc tacaaaacta
    37381 tcgagttttt ttagtacttc tttgaattct tcagtattct ttttcaacac taaagctaaa
    37441 gcttgacctt ctttttctcg actcgcctca atagaatcaa gtgcgctttt caatgtatct
    37501 agagctaact gtgccaattc ttccatgctc tcttccggtt tttcttctac cgttacgaag
    37561 ttttcgtcca gcacgaagcg ttccgccatc cgactaattg acacttgatt ttgactaccg
    37621 tatcgttgtt gaattccgtc tgtcaattga gtgataagtg aatcaatcaa ttcccagttg
    37681 atgacgatct gtttttcgtt tttgccaata taagctagat tcacaaatac ttcgattcga
    37741 ccgcggctta actgctgttt aataagctga cgaaaatcgt tttcaacaaa gttcaggatt
    37801 ttaggacttc gtatttgaac atctagaaaa cgctggttga cactttttat ttcaatatct
    37861 aactgatacg cttttgtttc gcggatggct ttgccaaaac cagtcatact tttcattcga
    37921 cagggtcctt cctcattaca tattttctat tgctttttgc agttgttcaa actcttcgtt
    37981 cgtcaatgtc aggcctttcc ccattttttc atggtctggc gcccaatctc gaagatcgaa
    38041 ttttggcggg cgaccattcc agctgactag gttcagttct ttacgccacc ccttgttgtt
    38101 ttctgacaat atagcgattt cttctacgat ttcatatgaa aattcttgtg ccatggttgt
    38161 tcctccttga gataatataa atataggatc agtaatgtgt caactggttg cttaagcaat
    38221 tcatgctgca aacgatcctt gcgttttcct tgtagcagat aatcaaaata aacagttttc
    38281 gtgcgctcat tttgtatttt ttgctggtat tttcgatacc ttcgccattc ttcagtactt
    38341 aattgaccaa tttgtgccaa taaatcatcc ggcatacttt ttatatcttg ttggcagatc
    38401 atttgaatca tcatgtctac ttgatagatt tcattgggca gtaaaaaatg tttttttgct
    38461 aaaagttcta gcatcgccaa tccgcatgtc tctggtacga ccctgttctt atataaatta
    38521 cgcagttttt gccaaaattt tatgcttttg aaaaaagata attctaactg aatttctttt
    38581 gttaagtaag acaaaaatcc tcgccagtat gttcttgcag gtttaacaga atgaaatgta
    38641 taccagttcc catagtaata gcctaatcgt tttttcattt ccagatagcc taattgtaaa
    38701 tttaattggt tcctttggct atcaaaaagc agaaagctgc ctagtgtcca caatgtttga
    38761 catttccaat ggataaaaat gtctggcata cgaatttttt tcgctgggcc tggcccttgc
    38821 acatctacga caaatgcctc tgttgctcct ttgttaatcg caatatcaat aggtattttg
    38881 ttgcgatacc caccgtctgc atatttattt tttccgatct ttcgataagc catagcggga
    38941 taaaatgaag cagaggcgag tatccaatct ggtatttctt ctgtttttgt ctgttggata
    39001 tcagtcacta cttccgctag ttctggcagt ttagtagaca ccgtatataa acgtattttg
    39061 ggattatgtt tgagtaagga caaatccaaa ttgtctttga ccatcttttc caatggagca
    39121 gaagaaaccc cgccttcaat tagtgctgtt ttcgtcattt ggcgtctttc tcgatagaag
    39181 cgttcacgaa ggtcttcaac tgcagccatt tctggtaacg caagcacttg tcttgtcgac
    39241 agctttttcc ataaattcac agcttttttc atatctcctt gtaaaatcaa tgcgccgttc
    39301 aacgcaccta ccgaggtacc tgtgataatc tcaaagttga tatcatgttc cttcagcgcc
    39361 tgccacacac caatctgata agcaccgtgc gctcctcctc cacctaaaac aagtgctgta
    39421 tgatagacca gctttttatt taattgtgtg gttccttgat agccgttttt atgaaaaata
    39481 cgcaaccatt cttctgatag aggagattga aataaaaaac aacagttttc tttaataaaa
    39541 aaacgagcgc aagcttccat ataccgaaaa aattttttcc attcaaaagt ttcaccttca
    39601 atcaacagat tattaaaaat aacatctcct ctacttttag caatagtaaa atagacggta
    39661 tattttttcg atactgcttt aaaagcacat tgcttttttg cagaacgcca aaaagcgtct
    39721 ctactttctt gatgactttg aaaaaatgga aggggttgtt ttgcttgctg gataggcgaa
    39781 tacagcgaca ttttctgtga aaagtaaact ttttttatct ccatcgcgct ctcctttttc
    39841 ttcaagtata ccagtacgtc ataaaaaggt catgagtctc gtattcgact ttgtagattc
    39901 cataagaaaa caattcataa aacctccttt aaataaaaaa atacaaaaat cacaaaaatt
    39961 aattgaaaat gagagtgttt tcaatgaaaa cgtgattttg taatggaatt ttaaaataaa
    40021 agaaacaagc ttttttaatg caaatgttgt aaaataaaag agattaagag gaaataagag
    40081 gaggaacatg atgactgagt cagtttatgt acatattgat accacaagta atgctgtttt
    40141 aacaaaaggg ctgactgcta ctgactttgc aaacagtatc gtgcattttc ctcaaaactt
    40201 gctcttgctt gatccatctg cttctgctgg agaatatgaa tcccatacag ggctgaaagt
    40261 aatccgtggt acagaaaata ttcagcgttt cttttcctct tctcgtaaca ggcgtggatt
    40321 cgatgaattg aagtggatcg actttactga tttaacgatg ctgaaagaat tgacaccttt
    40381 agaaatttca gaattattat attttggtca tatgaaaaca catcttcatt cgccgttttt
    40441 ttataaacta cagaataatt ttgtatactt cgatcttaat gatcagctga atcggattta
    40501 ttatcgctat ttagatgaat tttataggat ttttgctagt aaattaagtt cgatcttgtc
    40561 agataagatc aatgtaaaaa aatctttttt caaaaaagaa acacctgttg aaaaaatcag
    40621 tgtggaatta ttgaaagaaa tgcgtaattt gatgcaggaa gggatcattt tttcttttga
    40681 tcaagttggt ttgatcgatg gtaaatatcg tatcccaatc tatgtgattg aagattatat
    40741 ttggaaatta aaaggcaccc ggtatcgaga agaggaaatc atcgctactt taatctactc
    40801 tgtcaccgat cgacactggc aattggtaga agatgccgat ctttcttatc tgccattgaa
    40861 gtagatctat ttcttatcaa aaaataaaaa ggaaacaaga aactaggaca tgattctgtc
    40921 tcaagtttct tgcttctttt ttatttacag ctcctgtcgt ttatctggca caaaaatcga
    40981 aggagtcgtt tgttggatcc ctaataaaac gatgatcgtt acaatagctg tactcaaccc
    41041 gctggcacca ttcattacga aagaaaagag ccaaggattc atcccccata gtgcatacgc
    41101 tccccagaaa accactcctg caataaaatg ccaaaaatat ctggaaagtg cgccaatcaa
    41161 tgcaccaaac acgatgcttt ttctaatttg tttcgtatta tcgtctacat agcctttttg
    41221 gacatttgaa gccatgagcc ctgctgcgcc tgcaaaagta taggcaatcg ggtactcaat
    41281 caacacttga acgacactta aataataaac ttgtcccaac ggaaaatgta ggatgcccca
    41341 aatgaaacct gccagcatcc ctgcctttgt ccctcttctt aacgcaaaaa gagtcaatgg
    41401 aatctgccct aatgaaatag aaaaactgct gccgatctgg atcggaatca acgacaacac
    41461 catcgctaac gctgcaacaa tcgttccttc tacccaaact ctattttttc ccataaaaaa
    41521 acttcctcct ttgaataaaa aagtcacaaa ggaggaagca gctttcgaaa atagtcaaac
    41581 agctgtttat tcacaattcc tacgctcgca taaccgatac aggtacgaag ggtttagaaa
    41641 tagattcaat ctcagcccaa ttggactccc ctttgtgagt ttatttatta tttatttttt
    41701 gtttcaaaca atggactaac tggtttgttt tcatggatac gtttgattgc atctcccatt
    41761 agtccgccaa cacttacttc atcgattttg tcgattttac gatcttctgg aagatagatc
    41821 gagtctgtaa caactagacg ctcgatagct gaatcttcta ttctttgtaa agcagggccg
    41881 gataatactg gatgtgtaca tgaagcatac acactagttg caccagcttc tttcaaagca
    41941 ttggcagcca atgtgatcgt acctgctgta tcgatcatat catcgatcag tacacaagtt
    42001 ttgcctttga cttgtccaat gatattcatt acttctgcaa catttgcttt tggacggcgt
    42061 ttgtctatga tcgcaatcgg agatttcaaa aattccgcta atttacgtgc ccgtgtgaca
    42121 ccaccatggt ctggtgaaac aacgacaaca tcatcgcctt tgattccttt ttctaagaaa
    42181 taattggcaa taagtggcgc acccatcaaa tgatcgactg gaatatcaaa gaaaccttgg
    42241 atttggactg catgaaggtc caaagtcagc attcttgttg caccagcttt ttgaatcatg
    42301 tttgctacta atttcgctgt gattggttcc cgtgcacgtg ctttacggtc ttgacgcgca
    42361 tagccataat aaggcatgac tacgttgatt gtttttgcac ttgctcgttt cagtgcgtca
    42421 atcatgatca gcaactccat caagttatca ttgactgggc tgctcgttga ttgaataaca
    42481 tagacatgcg atccacgaat actttcttca atgtttactt gaatttctcc atcactaaat
    42541 tgagtaacag atgattttcc taactcgacg ccgactgctg ctgcaatttt ctctgctaat
    42601 gggcgattgg aatttaacgc aaaaattttt aatcttgggt caaagtaatg ttttgacatg
    42661 gggacctcca ctttcttttt cacaactcta gaattctacc taataaatag tagtcatatt
    42721 ttttaaataa atcaagtgat tttgcaaaag tcttagttta gataagggag tttttttgca
    42781 tatccttctt tattgacttg tcttgctctc gcaattgcca gcgcatattc tggaatattg
    42841 tctgtaattg ttgacccggc agcaatcgaa ctgtttttag ctacctcgac aggtccgatg
    42901 atattcgtgc tggaaccaat aaagctgtgg tcgccgacag tcgtatgatg tttgttcttt
    42961 ccatcatagt tgacgaagac aacaccacat ccgacattga tttcttctcc cagcgtagca
    43021 tcaccaacat aagtcaagtg accgacttta gttcctttgc cgatttgagc atttttcact
    43081 tccacaaagt ttccgatatg gacgttttcg ccaatttcag cttttggtcg taaatgtgca
    43141 taaggtccca catctgcatg tttcttgaca tggctgcttt caatgactga gttttcaatg
    43201 acgacgtgat cctcaatcct actgtctacg atttttgaat gtgacccgat gacacaatca
    43261 gaaccaatga ctgtatttcc ttggatttgt acacctgctt cgatcaatgt gtctggaccg
    43321 atttctacgc ctgcatcgat gtatgtcgtt gctgaatcga caaagcttac gccattgacc
    43381 atatgcatct ggttaattcg ctttctcatg atctcatttg ctttagctaa cgcgatccga
    43441 tcattgactc ccattgattc ttcaaaatca tccgtttgat aagcagcaac tgtatggcct
    43501 tcttctttta agatttcgat gatatctgtt agatagtatt caccttgagc attgtttgtt
    43561 ccaactttgc ttaacgcatc gaacaaagct tcgttgtcaa aacagtaagt tcctgtattg
    43621 atttcttgta caagagcttc ttctggtgtt gcatcttttt gttccacgat tttttctacg
    43681 atcccaatat ggtcacggat gattcgtcca tatcctgttg ggttttctgc ttgtgccgtc
    43741 aagatcgttg cacttgcatt ttttccttgg tgatattcaa aaagattgtt cagcgtttca
    43801 gtagtcaata atggtgtatc gccactgata actaacgttg ttcctttttt tccttccaag
    43861 aaagaagcag cttgcaaaac agcatgtcct gttcccaatt gttctgcttg taacgcatat
    43921 ttactgcgtt ctcctagttg tgctttaact tgttctgctc cgtgtccaac gatcgtaatc
    43981 acttcatctg gtttcgtttc actgacccga ttaatgatat gttctaccat aggtttacca
    44041 gaaacgggat gtaaaacttt atataatttt gacttcatgc gggtaccttt ccctgccgcc
    44101 aaaataattg cataacgtgc gtccaactgc gtcactccta aatatagttt gctgatttca
    44161 gtttatccca gtatacccat tttttcaaca aaacaagtga ttttctttta tcccagcgtt
    44221 ttctggataa aaaaatagat agaccataac gggcctatct atttggtaac cggtgctctc
    44281 ctgccaactc ctcggtcaca agtcacacta ctcataaaac atgaaaagct gttttttgaa
    44341 atcgtttctt gtgacttaga gttaaacggt aggcttacaa cctcttcatt aaacgttttt
    44401 caatcagctg accttcggta ttagctcaat aaacggcaaa atatgaggag caatttttcc
    44461 gtttattgag ctaatactca ggacaggacg ctgatttcac aacctctaga taaacggtgt
    44521 tcaaaagcca gtttctcggt attaagccaa atttatgaaa aaatgtggaa agccattttc
    44581 acaaaatttt tcttactact cgaaactaag cggctttttc acaacctctt attctgaaaa
    44641 ataatttcct ggcacaaccg taatattttt cgtttgagta tctacatctt tgacataaag
    44701 cagtgacgta taatcatcga tggcgcgatg accgttgaat tttgattcgg caaatacagt
    44761 gatgccaaca agttctgatt cgaattcttc aatcaagctc ttcattccgt tgactgttcc
    44821 gccgcctttc atgaaatcat ccactacaag gacacgtgaa ccacgtttca aacttctctt
    44881 agacagttcc atcttttcaa ttctttcaga agatccagac acatagttga cacttactgt
    44941 tgatccttct gttatttttg agtcccggcg tacaataacg aatggcgcat tcagataata
    45001 ggacacagct tgggcaatag gtacgccttt tgtggctaca gtcattacag catcgatttt
    45061 ttcaccaagg tatttagaag caatgattct tccaacttga cgcagcagtt caggttctcc
    45121 taacaaatca gagagataaa catatcctcc tggaagcaga cggtcttgtt ctgatagacg
    45181 ttctgctaga gattcaatat attttttcgc ctcttcatat gaaatttcag ggatgaacaa
    45241 tacaccacct gcagcacccg ggattgtttc caaggttcca taccctcgtt ctttaaatgt
    45301 tttcttgatg attcccaaat cttcactgat agaagattta gcagattcat aacgttcggc
    45361 aaagcttgtt aaggaaatca attcatgcgg gtgattcaat aaatactgtg tcatgtcaac
    45421 tagacgttca ctgcgacgta ctttcaccta ttccaactcc ttttaagaaa ttttatcgtt
    45481 ttacattata taagtttttc ttcaaaaatt cgagcatttt atgaaaaaat aagaaaatag
    45541 ttcggtattt attaaaataa aaagaagcct ataaaaaatt tttcgttttt ataaggcttc
    45601 ttttctttat aatttagtta ttttttcttc tgaaggcaac caatattcgt ttgatgatat
    45661 ttacaatcag gaacaaagca ataaagacta atgtgattgt tgctcctgga ggtgtatcta
    45721 gataaaacga gctagttagt cctgacagca tccctagaaa tcctacacaa acactgatag
    45781 caatcacgac attgaagctt ttgcctaatc gcataccgat tgctgcggga agaaccatga
    45841 ttgcggatat caaaagtgca ccggcaatcg ggatcatcac agcaatagcc acaccagtaa
    45901 tcacattgaa catcatcgac atcaaatgaa cgggtagccc atccacgtgg gcggtatctt
    45961 catcaaaagt cagaacatac atcggccgtt tgaataaaaa gaacaatata ccgatggcaa
    46021 cgaataagat tcctaaaaag atgacttgct cccatgtaat ggtcacgatc gaaccaaaca
    46081 gataggattg gatacttgtt gctgaattcc ctccgctcaa attcatcaat actaacgcta
    46141 gcgccaaacc accagccatt aaaatggcaa tggagatttc tgaataggtt cggtagatcg
    46201 ttcgcagata ctccaatatc acagctgcta atacaacgac tagcatggtc gtaagtgtag
    46261 gattaaggtt caaaaagaat cctaacgcta cccctgctaa agagacatga gaaagcgtat
    46321 cagccatcag cgattgtctt ctgatcacta aaaacacacc tagcatcggg gcgattccgg
    46381 caatgaagat agcggcaaga aacgctcttc tcatgaattc atatgaaagc aacgccattg
    46441 agaatcctcc tttctcacca gtcggatctg tctgtccgca tactgtttga tatcttcatg
    46501 atcatgggtg atcattagaa ctgatttccc atggacatgc gcactatgtc ggagaagacg
    46561 ataaaattca tttctggatt cttcatccat acctgttgtt ggttcatcaa gaacaaataa
    46621 gtcagggtca gtagcaaaaa tacgcgctaa actgattcgc tgcttttgtc cgccagacaa
    46681 ttcacctatt cgccgatttc gcatatccca catccctaca gcttctaaag cacgctgtac
    46741 atgttgatga tctttttcag agagacgctt gaaccagcgg cctcttggat atcttcctga
    46801 acggactaat tcgatcaccg tactaggaaa acctgcattg aaagatgcaa cttgttgagg
    46861 aatataaccg atacttaatt tttttccttc ttgatttttc tcagcaatct ttatcattcc
    46921 ttttgaaggt ttcagtaatc ctaacgatgc tttcaccaag gttgacttgg ctgctccatt
    46981 ttctccagtt aaaatgacaa attcacccgc atctacatga taagagacat cttctagtac
    47041 aggttcatca tcatagtaaa aggtcaaatc agatatttcg atataacgca ccttacaacc
    47101 tccattctaa aacttaattg atactttctt ttaaagcttt cagatttttt tccatgacag
    47161 aaatataatt ttcgcctgct ttgatttgtt cattagtcaa gctttctaaa ggattcaaca
    47221 cttctagctt cactcctgtt tcattcgaca atgtctcagc taccttcgaa gaggcatttt
    47281 cttcaaaata aatcactttg atgttgtttt tcttcacaaa ttcttttagc tcagctaagc
    47341 gacttggtgt gggttcctga tctggtgaaa cgcccgaaat cgctacttgt tttaagtcgt
    47401 attgattcgc taaataagca aaagcagcat gctgagtgac aaatgtgcgg tttgttgcat
    47461 cttttagctc atcggagtat ttttgatcca gtgcttcaag ctttttgata taagcatcgg
    47521 cattcttcgt aaaagtttcc tttttatctg gatatttttc gcctaattct tcagcaatcg
    47581 ttctgacttc ttttatagct aataccggat cgagccaaac atgtggatcg tattcatgtg
    47641 aatgaccatg cccttcttct gattcttcat gttcgtctgc ttcttgcgat tctaaacggt
    47701 cgattccttt agctgcttcg atcatcaagg tctgtttgct atccactgaa tcctttaacg
    47761 aatccacaaa aaattccatg tctgaactat tatacacgaa aacatctgca tcggaaattt
    47821 ctgctctttc ttttgcactt ggttcgaaat catgcggctc cgttccagca gggatcaaaa
    47881 gtttcacgtt tccttcatcc cctacgatct cttttgtaaa atcatacatc ggataaaaag
    47941 tcgtaacgat cgtcaaatcc tcttttttgt ctgcttcctt gttcgtattt ccacatgctg
    48001 caaacagtaa agcacctatc attaccgtag ctgtcaaaag atattttttc atttatatgc
    48061 tcctttcaat gaacaaatcg gaattttacg atttattaac gtgaactaat gtatcaaaat
    48121 aaaaaaacac agtcaataga aatccatcaa tttctgattt aagcatgtat attcttgatt
    48181 aaatcgtaaa atggatgagt tgctgctcat atctagccat ttcactacaa taaaaaactg
    48241 aagaatacca ttttaggatt cctcagtttt tcatcaccta ttttacttct tatattttat
    48301 tgcagggttc gtactaaata aacttcctca caaaaacctt ttaacccatt atatactctc
    48361 tgcgctcttg aataatgttg acacaaggca aaaacggttg gtccgcttcc actcatcaat
    48421 gcagcatctg ctccgtactt gatcatacgc tctttgattt gatggatgac tggatgccgc
    48481 tttcccgtta tatcttctaa actgtttccc atttttttga tcatctgctg ataatcttgc
    48541 tctcggatgg cttcagcaag tccgtggatg tcttgatgtt ggatggcttg cgtatctact
    48601 tctctaaaca ctgtacgtgt agagacactc attcttggtt tgaccaacac aacccagcac
    48661 tgtggcatag aaggtaatac ttggatttct tcccctttgc caccgacaaa tgccgtactt
    48721 ccgtagacac agtaaggaac atctgtaccg acttccaaac cgatttctgc cagttcttct
    48781 aacgaacagc ccagattcca taattggttg atccctctta gagcagctgc acagtctgta
    48841 cttcctccgc ctaatccagc tgctactgga atattcttct taacaaagat cttgacaccg
    48901 gttttgatat tgtatttatt ttttagcaat aaagcagctt gataaacatt gttccgttca
    48961 tctaccggta aaaatgcttt atttgtttct acaatgattt tgtcttctgt tatttcttca
    49021 aaaaatagat gatctgccaa gtctacgctc gacatcacca tttccaactc atgataccca
    49081 tcttgccgtc tatataatac atccagtcct aaatttatct tggctggcgc tttttcaatc
    49141 aattccatcc tctcacccat tcgaaaagtc taagtcgcaa gtattctatc atagggaaaa
    49201 ataaaagtat agcttatgtc tgcctagtgg ttgattttgt catcgtttca acagctttca
    49261 cagaattttc tcttcataat ccaaacaagt tctagtgatt cttataattc tgtgagcact
    49321 tcttctgcca aactcggaac atttcctttt tcttttccta tcagactgag tagctgcatg
    49381 gtagcaaatc cgaaattact tcctttccta taagcaaaat aacgtccttc ccaatgatcg
    49441 acatatttta atttcttttc ataagcagac tggaaggaat agaaaaaatt accataatta
    49501 tatacgatat gcatgacttt ttcttgaaag aaagaaaaat ctgtttctcc aacattagct
    49561 aatggagcta agcctagatt ggcttccgag tagcccattt tctcgtatac aataaacaga
    49621 ttggctaaca gatagtcagc taattcctca gattctttag gtaagacccg caacaaatca
    49681 tatcccgcct gcttcttcgt catcggctga gcggtgatga agccaacgac tttttgatct
    49741 ttacggacaa taccgacacc gctagataat acatatgatt tatcaaatcg tcccacagag
    49801 aagtattttt ctcgtgcacc ttgaagccat ttttgtgaaa cttcatttac agcttgatgt
    49861 aattcttccg gcagttgatc gtaccaagta aaatggtatc cttctttcat caaacgttga
    49921 tattcaagtt gctgactcag ccgagacaag tcttctgttg ctagatcaac aatccctgct
    49981 tcacctactt tcgtgaagtg aaaacccaat tcatgaagca tcactacgta gctttctgat
    50041 atcttataaa acgcaagctg atagcctaaa caatctgctt gtttcataaa ctgattcgtt
    50101 gctgcccgca tcattttagg attgccaact ggatctccta aaacaaagca tttgtttcct
    50161 ttgatctgat aaccaaaaac gacttgatct tggtcgtctt cttggtaata aaaataactg
    50221 taacctgaca agcgaagctg atggcttgta gctgtgcctc cgtatgtttc caataaacgg
    50281 tcaaaacggt ctttttcgta ctcactgcca agcttcgtag tatcttcagc taaatattga
    50341 taaagcgcca ctaaagctag caatgagatg cccaaaccaa taagccctga gaaccacaca
    50401 tcatctgaag gaaacaaaat aaaatgattt cctgttattc gaccgactaa aaagcgtccg
    50461 ctgtgatagc ctgcaattgc gtaaacgata aacaaaaagc cgaacaagct agcatcaaag
    50521 attatagctc cccaagaata aacgaatttt gttcgataaa attcttttct ggataaccac
    50581 acagctaaca gaatcaagac atataccaat atcaactgcc aagataacgt acgtgtgatt
    50641 gtatttaaaa taccaaaaga tagtaaagca atcgtaggca gaaatgcctt tttgactttt
    50701 actgaaagtg ctctagcaag gccgagtaat aaaaagccaa ccaatagatt cactgtctga
    50761 tctaaaaaat tgaatgagaa cggcaataat acttgaaata gccgactgat tgcagacaga
    50821 ttagtgattg ttgatagaag taccatcatg atccccgcaa aatagacagc acttacgagc
    50881 acgatatgcg caaccttttg gaaaaacagt cgcggaatgt tatctaaaaa tcgattgatt
    50941 ttgactcctg tatgtgtgat gaacaataga agtcccgtca taaaaggtag aacataataa
    51001 aacaaccgat aatataatag ccaaactact gctacatctt gccgaacaga caattgactc
    51061 attcctaaga tcatcaaaac gtcaaaagtc cccattccac ctgggaccat cgtcagcatt
    51121 cctatcaatg tagcaatcat gaacatagga tagatcgaaa gcatcgaaat atctattctc
    51181 atcaacttgc cgatgatcaa aaataccgca agggctcccg tccattgtcc aaaggatgcc
    51241 agtagcagac caagcaagcc ttttggataa aactcgaaga acaatgtctt tctctttaga
    51301 taagcaaaaa agaaaatgac tggtgctaaa aaactaccgc cgaaaagcca gatccaatag
    51361 cttcgaaaaa tactttctga ctgaataaaa aagacatcaa taaatgtaag aaacgaccat
    51421 acagacaaac cagtcagcat aaatagtgcg acttttgata cagtggctaa aaccattttt
    51481 ctatttgtct gctttccata aaaactagcc cgcagacttg ctcctaccac accaccaaaa
    51541 ccggcgagat tgttgatcgt gtttgttacc cacgcagcaa gaagaaactc ttttctttcc
    51601 atttttggtt ttccttgttt ttctaagact ctgatcgtca cccaatcata caacaacatc
    51661 ggagtgacac ccagcagtcc agccagaatc atgagcagga tcgtcatgcg gctttgttgt
    51721 cccatccttt gccaaatatc ttgccaactc atgccattag caatatttgc gacttgattt
    51781 gccacaaaga ctaacacgga gccgaaaaaa atcagtttta aaatcagact atgttccttt
    51841 atccattgca gcatcaagta tcgtttattt ttcacatttt cccattccta acttgtatac
    51901 ttttgtgtca taccccaatg ttatcttatt tattttaatt tttccacttt agacctgttc
    51961 tttgcttgcg atgatagaaa aagaaagata tcctaatgct aaaggagagg ataacatgaa
    52021 agtaggattt atcggcacag ggaatatggc acaagctatt attgatggtt tattaaaaaa
    52081 aggaacagtc tcgaaagaag aggtgttcgt ttccagtgga cattttgaaa atgcacaaaa
    52141 atttgctgct gaaactggcg tccatgcatg caaaagtaat caggaagttg catcacaatc
    52201 agatatcgtt cttttagccg tgaaaccaaa aatcattgaa aaagtattgg aagaactaag
    52261 agaatatagc cagaaagtct tattcatttc tattgcttca ggaaaatcat taatagattt
    52321 agaaacagcg cttgaagatc ctcaggcctc catcgtacgt gtgatgccga atgtcaatgt
    52381 cagcgtagga gcaggcgttt ctgcgatttg caaaaatgaa gctgccacgg aggaacagct
    52441 tcagcaagct acgatgattt ttgaagcaat cggcagcgtc tatcctttag cagaatcaga
    52501 tttcagtaca ttcattggcc ttgctggcag ctcacccgca ttcgtttaca tgctgatcga
    52561 tgcgatggga cggactggtg tactgaatgg tttgtccaaa cagacagcca ctgagattgc
    52621 tgcaaaagca attatgggtt cttgtcaaaa attcctttca agtgataaaa atccatggga
    52681 acttgtagac caagtttctt ctcctggcgg tacaactgtt gcaggtgtag ttgccttaga
    52741 agaagcagga ttcatcccag caatcatcaa aggaatcaat acgacgatcg agaaagatct
    52801 tgagatgttg aataaaaaat aatgatagca atgttttttc gtgccttaca aaaaaagaag
    52861 agctgtggct tttgccgcgg ctcttctttt gtatttttta ttatttagca taatcagttg
    52921 cacgagtttc ccgaataacg attactttga tgtgtccagg ataatccagt tcttcttcga
    52981 ttcgtttacg aatatcacgt actaaacgaa cagcgtctaa atccgagatt tcttctggct
    53041 tgaccattac ccgaacttcg cgtccagctt gaactgcgta acttgattct acaccggcga
    53101 agctgttcga aatattttcc agacttctta aacgacggat gtagttttct aatgattcac
    53161 ttcgcgcacc aggtcttgct gctgagagcg catctgctgc agctacaagg acagagataa
    53221 cagaagtagc ttccacatct ccatgatgag aagcgatcgc gttgattact actgggtttt
    53281 ctttgtactt ggcagctaat tccgcaccga tctcaacgtg agatccttca atctcatggt
    53341 caagtgcttt cccaatatca tggagcaacc cggcacgttt tgctaattgg atatcttcgc
    53401 caagctctgc tgctaagata ccagctaatt ttgcaacctc gaccgaatgc ttcaagacgt
    53461 tttgtccata actagttctg aatcgcagac gtccgatgat cttgattaga tctggatgaa
    53521 gtgtatgagc accgacttca aacgctgcct cttccccgta ttcgcgaata cgttcatcca
    53581 tttctttacg agatttctcg accatttctt cgatacgcgc tggatggatt cgtccatctt
    53641 gtatcagttt ttccaaggtc atccgtgcga tttctcttcg gattggatcg aatccggaaa
    53701 gaacaactgc ttccggtgta tcatcgatga ttaaatcgat accagtcagc gtctcaagcg
    53761 ttcgaatatt acgaccttct cgtccaatga tacgaccttt catttcatca ttcggaaggg
    53821 taacgactga aacagtcgtt tctgaaactt gatctgctgc gcagcgttga atcgctaatg
    53881 ataataagtt tttcgctttg cgatctgctt cttctttggc acgctgttca gattctttta
    53941 ccattagagt taactcatga ttcagctctt cttcagtcga tttcatgatg acatctcgtg
    54001 cgtcctcttt tgataaacca gcaatttttt ctagttcttt ttgttgatct tcaatcattt
    54061 tttcaacgtc tttttctcgc tcttcaatta attgctgttt tgaactaagt gcttcttctt
    54121 tactttcaag tgagttctcg cgtttttcta acgagtcatc tttacgattc agcgtttgtt
    54181 cgcgttgaat caaacgattc tcttgagatt ttaattctaa cttactttct ctcagctcac
    54241 tttcaacttc agaacggtat ttctgatttt cttccttagc ttctaacaat gcttcttttt
    54301 ttagagtttc tgcttctttt tttgcactat cgatgattcc agcagctgat gattgtgcac
    54361 cagctatttc tttctcgtga cgtgactttg cgaccataaa ccctaaacca agaccgacaa
    54421 ttaaaccgat gatagcgagg agaatgttga ataccataaa atccacctcc atactatctt
    54481 tttcattttt atcatctata cagcttctat gataaattat tctaaactcg tcaatatgca
    54541 tcttcgcata tatgtgtaca aaaaacatgc acaactttat tctaatgttt gtaccccgta
    54601 gtgtcaactt taaataaaca aagaaacggc tttctactta ttcattaaac gatggttttt
    54661 aaaataaatg acactagttt catataggta aacctttaaa ttgagaaact tttctattaa
    54721 attattcttt gtagatgaac agatattttt attttatgta cccactcgta taattaaata
    54781 actcacgatt tctttattca ataaaaaaac ggcaggtcaa ggagagtacc tactcttcag
    54841 accagccgtt tttgtttatc ataattttta ttcttctact aaaggcaatt cttcttgtcc
    54901 tttttcatct tctgggacag cgacttcttc accaatacca taagctgctc taactagagc
    54961 agaaacttct gccatcattt caggatgatt agccatatag attttagcgt tttcccgtcc
    55021 ttgaccaatc cgatcttctt tataagaata ccaagcaccg cttttatcaa caatgtcttt
    55081 ttctactgcc atgtctagaa gttctccttc ttgtgagatc cctaaaccat acatgacatc
    55141 tacttcagcg attttgaaag gaggtgctac tttgtttttt acgactttga tctttgtacg
    55201 gttaccaaca atatctgtac cttgtttcaa ttgttctgca cgacgtactt ccaaacggat
    55261 cgttgcgtag aatttcaatg cgcgtcctcc aggagtgatt tctggattcc caaacatcac
    55321 gcctactttt tcacggattt ggttgatgaa aatagcgatt gtttttgttt tgttgatcga
    55381 accagagagt ttacgcaatg cttgagacat caaacgtgct tgtaacccga catgtgagtc
    55441 acccatttcg ccgtcgattt ctgctcgtgg aactaaagca gcaactgaat cgactactac
    55501 gatatctaca gccccacttg atactaaagc atcagcgatc tctagacctt gttctcctgt
    55561 gtcaggctgt gaaagaagta gttcatcgat attcacacct aatttttgtg catattgcgg
    55621 atctaacgca tgctcagcat caatgaaagc ggccgttccg ccattttttt gtacttctgc
    55681 aatagcgtgt agtgcaactg ttgttttacc tgaactttca ggtccatata cttcgatgat
    55741 acgtccacgg gggtaaccgc cgacacctaa agcgacatct aacgcaagag agccacttgg
    55801 aattgtagag atttgttggt cgattttttc gcccaatttc ataatagagc ctttaccata
    55861 gctcttttca atttttttca gtgcagcatc taaggctgct ttacgatcat ctgccaatac
    55921 gataatcctc cttatattta caaatcagag caacatttca ttatccttat catatctttt
    55981 ttagtgaaaa aaagcaagaa aaaaccgaac aaatattcgc atttatcatt ttcttaaaag
    56041 ctgttttctc aaaagatcaa atccttgcat aaccgcatta tatctgatag cagcacgatc
    56101 tctggcaaag tgatattttt ccgaaaccac gccgtcttta gatgaaatag cgatccatac
    56161 tgttcctgca gattgacctt ctaacgtgtc cggtccagcc actccggtga aagctacact
    56221 gtaatctgtt cttgctatct cacgtgcttg aatggccatc tgctccacac attctttgct
    56281 gactgttcca tattctgcta atagctccgg atcaatctcc agaaaaccag cttttgtttg
    56341 gagcgagtag gtaacgaatc cgcctggaaa tacttcagat acaccagcaa tgtcgcctaa
    56401 tgcggcttgg aaggcgccgg ctgtcaaact ttccgctgct gttacagtct gcttgttttc
    56461 tttcagtagt tccactacga cttttgctag cgaattatca tcgccgtatc cataaaaata
    56521 ttcccctact cgttcttgga ttttttcttc taatgctaaa agagcttgat tccctgcctg
    56581 aacatcattt gttttcactg tcagtcgtaa tgtcacttca tttggtttgg catatggtgc
    56641 gatcgttgga ttgatctgtg tctcaatcag atcttttagc tcagtgacca gtcgcgattc
    56701 accaatccca taaaagcgca aaactttaga aattagtttt tcttcagaag gaaaacgttc
    56761 ctctagtaaa ggacgtactt gttccacaaa cattggtttc aactcattag gcggaccggg
    56821 aagaaggata tacgcatgtg tatctgtttg ataaaaagtc cctaaagcaa gccctgtacg
    56881 atttggtaga ggaaccccgc cttcgataat ctgggactgt tgcagattgt ttttggtcat
    56941 ttttcgatgg gttgtttcaa aatatgccaa taattttttg tatccttccg tattttgtat
    57001 gagagactta ccgaggtgag ccgcagttgc ttcttttgtc aggtcatctt ctgtcggtcc
    57061 taatccgcca cataagagta tgagttcact tcttgtttca gctagagcaa tcaactcttc
    57121 taaacgctgc tgattatctc ctacaactgt ttgataataa acttcgatac ctagatcagc
    57181 tagctgttct gacaagaaag tggcattcgt attcacgact tgtcctagta aaagttctgt
    57241 tccgacagct attatttctg ctttcattac ttcccgctcc cttatatagt atacgctgta
    57301 tttttatatt tttttattta aaaagagggc aaggagctgt gacaaaaatc ttgtcacagc
    57361 tccttgttcc aaataaacag tgttctcctg ccaaaacctc ggtcataagt cacaatatac
    57421 ataaaacatg aaaagctgtt ttttgaaatt atcccttgtg actcagagtt agacggcagg
    57481 ctcacaacct ctccactaaa cgatacttac attgatcctt tgaacacacc gctgtttttg
    57541 atgaagtaat ccacacctga atagatagta aagatcagac aagcataaag catgatttga
    57601 tctactggaa taccgatcag gctgaatggg atattattga taaacagcaa gataatagca
    57661 accatttgtg tagccgtctt gacttttccc ggccaagctg cagccatgac ttctccatgt
    57721 tcgacaagta gtaaacgcaa gcctgtgacg gctaattcac gacagacaat gatcgctact
    57781 acccaaccag gggcctttcc ttggtcaacc aacatgataa aagcagtcat aactagcatt
    57841 ttatctgcca acgggtccgc aaacttgcca aaattagtga caaggcctcg cgcacgggca
    57901 atttttccat ctaaccagtc tgtgatacta gctaaagcaa aaataactgc acctactagc
    57961 tgtgtgacag ccagtgtggt gtctcctacg ttaaattgtc cccaatctaa aggtaaacct
    58021 acaaccaaaa tgaaaatcgg gatcatacaa attctaagta cagttaattt atttggtaaa
    58081 ttcacactat cgactccttt cttccctatt ctacgtgact atcagcgaag tttccaccga
    58141 tattatttac ttttccaaat gaatttattc aaaggagtat ttttattgaa attgatcttt
    58201 ttgaattaac gaagaaggac cgcgacacag atttgtcacg atccttcttt tttataccta
    58261 caagtaaaca gatgcgtaaa ctaatttata cgatcattct gcgtattgaa gagtaaggtt
    58321 cacatttttt ggactctgat tgttttcccc tggattaact ggtacggatt ctccattcgc
    58381 tttgatcgtt gcattgcttg ctgcaccaat cgtgatggtc gcatttgttg ctgtttccgg
    58441 taacacggta ctctgtgttt catttgctgc cagtgtccct tgataaacat aggcattgtc
    58501 tactagcaca cctaaccata cacgattgac agcagtaaat tcaagggtta caggcttggt
    58561 agcgtctgtg atatttactg cgatggcact gccagaatct tctcctgtag ctattgccat
    58621 tttcttttct tcacttgatt ttgttgttga ttctactgta gaagactctt tggtctgcgt
    58681 gctttgctct gaggaagagg actcctcgac tgatccttct actttaagcg aagaagctgt
    58741 gtctccgatt attggatcag cttgacgatc ttgccaagtc atatagccca cgacaacgac
    58801 aatcgccaaa gcaaccaatc ctaataaaat gacaggaaga cttgtccaaa agcgacttgg
    58861 atgcgattct tcctgatgca ttgcttttct agaagtactt actgtttcag gctgcggacg
    58921 ttcagggacc gatcctgtca atttgctttt cccatcaaaa acatcgacta aatggtctcc
    58981 atcttctcct acagcttcag cgtattgccg aataaatgcc cgcacgtaaa atttaccagg
    59041 caattggtca aaatcatcat tgtcgattgc ttccaaataa cgtttttgaa tttttgtaat
    59101 ctgctgtaat tcatctaaag atattttttt attcaggcgt gcttgccgta atttttctcc
    59161 gatggtttca aaagccactc gttcatcttc tccagtctgt ttgattctcc gaagcatctg
    59221 ctgttgcaac gaaagacgtt atgtttgggt gctgtgacaa aagtcatgtc acagctcctt
    59281 ttttccgaat aaacattgtt ctcctgccaa ctcctcggtc acaagtcaca atatccaata
    59341 aatataagaa gctgtttttt gaacttgttc cttgtgactc ggagttaaac ggcaggctca
    59401 cgacctcttt cttaaacggt gttcaatcag ctgttcctcg gtattagctc aaaaaccggc
    59461 aaaatataag gagatatttt tccgtatgtt tctctaatac tcaggtcaaa acgctgattt
    59521 cacaacctct tccttaaacg gtgttcaaaa gcttgcatct gcggtaataa gtccaaccaa
    59581 gttaaaaaca ttaagaagct gtttttactt ggctgttctt attacttgat gcagggcagc
    59641 tttttcacaa cctcttggta cacggtgttc cagaagtagc ttcttcggca ataagccgaa
    59701 atatcacaaa aatttgagaa gcaattttcg taaattcctt cttatttctt gaagctgacc
    59761 acttctgtca caacctcttt gattacttcg tttgatttta ttttaatata agttgattaa
    59821 aagagagcat gatttgtttt attttctcac taatttttcc aacacaacta agaataccat
    59881 aaaagctgat tttggaggag gttcctacta caagtctaaa acgaaattta agacttttcc
    59941 tcataaaata ctggtttatt ttggtctcat gtaaaactcg ctgaaggcct cttcacttat
    60001 aaatgcttcc ccagctgcca gtacgtcttt catttggatc gaatcgatga tttcaggaag
    60061 atcaaataaa gttcgatcgc caaataaact ttgcgtaaat tgattggcaa tatattcgat
    60121 cgaatttaat gattggaaat attgtccaag cattttcttc ttcagcaaat cgagatttgt
    60181 ttctgacact tccggatcat cagcgaatcc aagaatgatt tctttgactt tttctgctgc
    60241 tttctctggt tcgtccgtat ctccgctgaa gtccgcaaag tgaaattcac gatccaagct
    60301 gaattcaaaa ccaaagctgt catcgataat tccttgattg tacatcgcta gatagtttcg
    60361 agaagtattg cctaaaatca tttggaacaa cagattgatt gctgttttat agcgtaacag
    60421 ttctgttcct tcttgcggca atgtgtctaa tcctttaata ccaagtacaa atttatctcg
    60481 tgtaatggct gcttccaatg cactttgttt gatgatttct tttgtgtttt cagggaaata
    60541 acgtacaatt tcttgctttg gagggaaatt ttttgcttct tggttttccc gaataagttt
    60601 catcaatttc tcaggctcca tcttaccaac tacaaataaa accatattgc ttggctgata
    60661 aaacgtccga tagcacgtat acaagtcttg agctgtgatt ttgtcgatac tttcaaccgt
    60721 gcccgcaata tcgatatgca atgggtgtgt aggatacaag ttatttagta tgccaaaaaa
    60781 catccgccag ttagggtcgt cttcatacat ttggatttcc tgaccgatga ttcctttttc
    60841 cttgtttaca gtttcttctg tgaagtaagg tgcttgtaca aaatcgatca gagtcgtcag
    60901 attcttttct acctgatccg tcgtagaaaa cagatagctg gttttcgtaa aacttgtgaa
    60961 agcattggcg gaagctcctt gtttcccaaa taattgaaag acatcaccat cttctttttc
    61021 aaacaactta tgttctaaaa aatgggcaat tccatctgga actttcacct tttctttttc
    61081 accataggga ataaattcgt tatcgatcga accataattc gtactgaaca aaccatatgt
    61141 cttgtgatag tcatttttag gcaacaaata cacggtcaaa ccgtttggca gtacttcatg
    61201 ataaagtgtt tcattgattt gctcatattc tgttttattc atttgcattt cctcctgcta
    61261 aaaagaagat tgcctgcaat tcgatttgct cagctacttg ttgtatctct ttgatcgtta
    61321 cgctctccat acgtttcaac cattcttcat cagataattt tgtttccggc agccagctgt
    61381 ccaaaaaaga agcttcgatt gccgcttgcg gagagtctag agaaagcaga tattggtttc
    61441 tcagcattgc ttttgtctga gacagttcca actctgttat ttcaccattt cgcaaacttt
    61501 ctaattgctc gtgaatcaga tgaagtactt gatttcggtt cttttcatcg ataccggttt
    61561 ggacactcat gaatcctcgg aacgtatcca cgctactaga ggcataataa gctagacttt
    61621 ctttctctct gacattcatg aaaagtttag agtgaggaaa accgccaaat aaaccgttaa
    61681 agaccattag cgcaaaacgt tctggttcat cgtaatacac atttgtctga taagctaggt
    61741 tcagctttgc ttgaacgatt ggctcacgta cttgtcgttc cttcacgata ttcacttgag
    61801 gttgggtata aaacagatct ggataaactg tttcccgcga ctcaaatggt aattgcccaa
    61861 ctgcttcagc tgctttgtcc ggatcaacat cgccgacaac aaaaatgtcg atttgatcat
    61921 ttgccatcat ttcttgatag taggacgcta gcgaactagc agtgatcttg tccaaatctt
    61981 cgactacgcc caaccctggg atcttttgtt ccgggctgtt ttcaaaataa agttcttgca
    62041 aggcatagga agcaaaagtt tgtttatctt ccttcaaact ttccaaatat agacgcagat
    62101 tgttcttttc caagtcaaac gtcaactgat caaactgatg atttttaata tttggataaa
    62161 ataaaacctc ttttaaaaaa tctaccgctt gagaaaaaag gtttggatca ttgacatatt
    62221 caccattcac aaaactgatc cctgcgttta gccaatgtaa atttcctttt tttctaactg
    62281 ataatccaaa gcttgcgcca taaagctctg caagttgcgc acttaacttt gtctgatccg
    62341 aataattcaa actatttgtt tccaacatgc tgctgagaag tgaacgtttc gtgatgactt
    62401 cttgttgcag acgcgcagta aaacggataa aaatccggat cgttttgtat tgttttgttg
    62461 gtaagacatg aagattcacg ccttctgcca attgatacac catatttata tgctcctttt
    62521 cacagttgat tgcccgctta ctcttttaat gggtcaacct gcctttttta cttattcctc
    62581 tgcttgaaat ttataagttc aggcaattcg ccgttttttc taaatgatgt tacactttat
    62641 agtatagcat agatgttttt tatcaaaaga aacagacggc taaaagcgta tattgttttt
    62701 ttatttttta tttttcgata tactaaagac aaataaaaga agggagggtc attcatgatc
    62761 aaagaattca aagagttcat catgcgtgga gatgtcttgg atcttgctgt cggcgttgtt
    62821 attggtagtg cgtttacaag catcgtaaac caagtggtgc aaggcttgat cacaccacta
    62881 ataggttggt ttgtcgcaat gatcacaggt acttctgatt tagaaggagc actttcaatt
    62941 cttgactggt cgccgacaaa aggcgtcaca tttgcgtttg gtaacgttat tagtgctatc
    63001 attacatttc tgatcactgg ttttgtctta ttcttagttg tcaaagcggc aaaccgtgcg
    63061 aagaatttac gctccaaaga agagcaagtg gaagtggaag aaactcctac tgcagaagat
    63121 tatcttagtg acatacgcga tcttcttgcc aaacaagtgg cagaagacaa tacacatcca
    63181 gtagagcaaa caaatctttc cgatacttct aaataataaa gaatctaaaa aaaagaaagc
    63241 cgattttcag tgtcggcttt ctttttttag attcttaact tgctacttgt tcctttttca
    63301 tgtcgatatg tggaatatca tcttccaaat acacatctga gattgccttg aatccaaacg
    63361 actcataaaa agcttgtagg tattcttgtg cgccgatcac gattggtttt tctggaaaat
    63421 ttttctggat catgttaatc gtttcttgaa caatctttct tcctaaacca tctttacggt
    63481 tgtcctgatg aacaaggaca cgtccaaaat ggatactctc cgcttcttca tatactcgcg
    63541 tgtaggcaag gatcttccca tcagtatctt gaaaaaacgt atgccaagct tctccatcaa
    63601 tgtcatcaac ttcttgataa ggacactgtt gttccacaac aaaaactgcg attcgttctt
    63661 tgacgatcgc gtaaaactct gctgttgaca attcttcaaa tttctttacc tgatattcca
    63721 ctaagcaaac gccccctgct tttttattct tagcataccg attcgcacac aaaaaagcaa
    63781 acgaacctta cgaatctcag gctcgtttgc tttttaaact gcttcagtta aaaattcgat
    63841 ctcaactgtc cgagtaagta catctgaata gctgtaagat actcgttcaa aagaattttc
    63901 ttcaggatcc aaatcaacaa cgaatactga aggataggtt tctgttaaaa cgccgcggcg
    63961 ttctgtttga cgctttcttc ctgtctgagc aaccaacatg attgggctgc cgatacgacc
    64021 ttctaaatct ttcttgattg aagctaaagt tgttggcata cagttcacct ctactatcaa
    64081 ctataccaca tatttttaaa attttcaagt ttaccataaa aaaaaaagaa atgcaataaa
    64141 aataaataag aagctcatta agatttttct aaattattcc tcattataaa atttcaagtg
    64201 tttttttatt ttgagtttta ctctcccgta agcgctcatt acttgcttgt gatccattgc
    64261 taaacattca gccatgtcta caaaatcaac gccttgcata taataatgaa aaattttagc
    64321 ttctattttt gaaaactgga tttcgcaatt ttcgattgct tcttttaata aaagatgctg
    64381 aattgctaaa ggttcttgca atgtgatctc tgctaaaaag tctggtcctt ctgtctctat
    64441 tttttggtcg attgagactt catctttttg atcacgacgc ttcagcgcgt tctgcttcct
    64501 caacagagaa tagatttgat tgcttataac gcttttataa taacttccaa agctaacacc
    64561 tttgcttttt tcgtaagttc tcaatgcctg atataaagcg atccttgctt cttgttccca
    64621 atcctcagaa acataatttc ttaaataata ggtgtttttc aaacgtagta aaagtggaaa
    64681 atatttttga tacagggcag caaatgcttc ttcgtcacca ttacgtgcag cgcgcaataa
    64741 ctcctcagac ataaaataat tcctccataa attcgtttat ggaggaatct tatcatcaat
    64801 gattcatcga actgattaga acgatatgtt atttattttt tctcattaaa tcgtccaatt
    64861 tttgggacaa tttttttaac tgctcttcat tccatggaga attacgtcgg aaatcaataa
    64921 aatgcatatc tttattttct tgggctattg ttttttccgt tttttttatc gtcttatata
    64981 attcataagc agaagttcgt aatgctcctt gggaaaagat catccactgt tctgctaaat
    65041 cgcttgtcgc cactgtcact tgtgtcaaac ggtcattcag ctgaccagcg attctctcga
    65101 tataactgtc agccgtttcg tcttctttcg taaaaacaac atccaactgg tatttcttat
    65161 aattttgttg gatgcccgga actagttggg catcgaagac aacgatgact tccattcctt
    65221 catatttcgc ataatttgat aaacgatgta aaagagcttc tcttgcatcc gccattttgt
    65281 cctgcttttt aagctggaca agttccggcc acgaaccaat catgttgtag ccgtctacaa
    65341 tcaacagctg ttttttcatg gtctactccc cctgacgttg ggaaaaagcc tgatacataa
    65401 gcaaaccagc agcaacactg gcatttaagc tctggacgtg cccggtcatc gggatcgtca
    65461 gcaattcatc catttctttt ttcaatcctg gactcattcc tttgccttca ttaccaatga
    65521 ctaaagcaat tgatcctttg gcattccatt tttggaagtt tgtgccggtc atatctgtgc
    65581 caaataccca aaatcctgtt tctttgagct gttgcacagc ttgtaccaaa ttcgtcacgc
    65641 gtgcaacagg gacatgttcg actgccccag tggaagtctt tgttacgata ggtgtgattc
    65701 caactgctcg gtgttttggt atgatcacac catcgacgcc acttgcgtct gctgtccgta
    65761 aaatcgaccc aaaattgtgt ggatcttcga tgctatccaa aatcaaaaag aaggggtctt
    65821 cttttttctc ttttgttcgt tcaatcagtt ggtcaaggga taaatattca taaggtgtaa
    65881 tcgcaagaac gactccttga tgcacgccgt gatcgctcaa agtttctaat ttttgtttag
    65941 gaacccattt gactggcaca gcttgttcac gtgcgatttc tttcagttct tctacttttt
    66001 ctcctcgtga atcttcttga agaaagagtt tattccctcg cccttgttgt agcgcttcta
    66061 ctgcggcatg gtgcccaaag acgaaattat cttctaatgc ttcttcattt tgatcagaaa
    66121 ctgactgatt tcgtccagtt tgctttttat ttgaatatct attttgtttt ttgtcttcaa
    66181 atccacgttt cgattgatgt ttgtttgttc tttctttacc catttttttc accaactttc
    66241 tgaatacacc agcggatcaa ttcttccatc cgctcggttt gttttgtcaa atgcagatag
    66301 cccatgagcg cttcgaaccc tgttgcgacc cgatacgtcg taacatcagc attttttgcg
    66361 ctcgtatggc ttttagcatt tcttcctctg cgataataaa gttcttcttc ttctgtcaaa
    66421 agatgttctt ccagcatttg ctgaatcaaa aaggcttgtg cattcgcaga aacataattc
    66481 gttgccgcac gatgcagctt attgggtttt gtttctcctt gatcaatcag gtggtcacga
    66541 acatagattt cgtaaatcgc atctccaaca taagccaagg ctaaaccgtt caattgtgtg
    66601 taatctctca tgcttctctt ctccatcggg ttccttgcgg tgtatcttct aataagatcc
    66661 ctttttcttt caacaaatca cgaatttcgt cgcttcggac aaaatcacga ttgcttcttg
    66721 cttgatttcg ttcttcgatc aattgttcaa tctcttcatc taataactct tctgctgtaa
    66781 ataaaatacc gaagatcgaa agccattgcg agaataaagc ttctccttgt gccaagacag
    66841 cggcagaaac tttcggctgg tctagataag tgttcagcca cttagcaagc tcatagacca
    66901 ctgtgatccc atttgctgca ttaaaatcat cgtccatttc ttcgatgaat cgctgttcta
    66961 aattttctaa caaagcgatt ttatccgcat catctgccaa attctctgag gcagtttctt
    67021 ggcggaattt caaattgtca aacgtattct tcagtcgttg taaattggtg gctgcttctt
    67081 tcatcgtcgt ttcactgtag cggatcggac gacggtattg tgtggtcgcc atgaagaaac
    67141 gcaagacttg tggatcaacg tttttgatca tttcatgtac agtgatgaaa ttccctagag
    67201 atttactcat tttttcatcg tcttcgccaa tcgttacata gccattgtgc atccagtaat
    67261 tagcgaattt tttccctgtt ttcgcttcac tttgtgcgat ttcattttca tgatgaggaa
    67321 attctaaatc ttgcccgcca ccatggatat cgatcgtttc tcctaaatgt tttgttgcca
    67381 taacggaaca ttcaatatgc caaccgggac gtcctttgcc ccatggtgaa tcccaagaaa
    67441 tctcgccagg ttttgcttgt ttccataaag cgaaatctaa tgggtcttct ttttgctgct
    67501 gttcttcccc tgtacgctga cttgctccga cttccagttc atcgatggat tggtcactta
    67561 atttcccata attttcaaat ttacgggtac ggtaatacac atcacctgaa gattcataag
    67621 catagccttt ttcaaccaac acttgaataa atgctaaaat atctggcata tgatccatga
    67681 ctcttggatg cagtgtagca ggttcgacat tcaacgcttt cgtatcttct tcaaatgctc
    67741 ggatgaaacg atcagcaact tctggtgctg taatacctaa ttcgtttgct gtacggatga
    67801 ttttatcgtc tacatccgtg aaattcgata cgaaatcgac tttatagcct cgatattcaa
    67861 aatagcgtcg aattgtatcg aaagcaatgg tgctacgagc atttccgata tggatataat
    67921 tatacacagt cggtccgcag acatacatac cgacatgtcc cttttgtata gggatgaatt
    67981 cttctttttt tctggtcaat gtattgtaga tttttatcat tctgatctcc tcactttttc
    68041 tcctttgatt cgaacgactt ttgctggtac tcctaccgct gtagcgtcgt ctggaacatc
    68101 tttcaacaca acggcacctg caccgatttt ggcgtttttt ccgatcgtta ccggtcctaa
    68161 tatctgcgca ttcgcagaga gcatggctcc ttgtttaaca gttgggtgtc ttttacccgt
    68221 ttcttttcct gtgccgccta atgttacgcc atggaataaa atcacatcgt cctcgatttc
    68281 tgcggtttcg ccaatgacga ttcccattcc atggtcgatg aacacgccag ttccaatcgt
    68341 tgctcctgga tggatctcaa caccagttag gaaacgccag aattgagcat tgatcttcgc
    68401 caataaaaag agccggtgct gatataaaaa atgagagaaa cgatgccaaa acaaggcatg
    68461 aaaaccaggg tatgtcaaaa cgatctcagc tgtcgaacgt gcagccggat cgtttctctt
    68521 tgctgcatca atcgctcggg taaaccaact catgactttc cactcctttc gtaagaattt
    68581 tatgtaggaa atcatcttct tataaagaaa agaagcgcct tttaaataag tttccttatc
    68641 aaaagacgct ttccgcgtgg ttccactttt cttcataatc aatgattatc tctgacagct
    68701 aacgcctgtt atacgttctt ggctactttg tttcctataa aacagttttt cctgttttct
    68761 gtgtttcgcc aagaccactc ccagaggcat ttcggcaacc attcattgat accttgcacc
    68821 acccggtatc tctcttgtaa tgatcagctg cttacttttt tctgttcatc gtgtttagtt
    68881 cagtacttga tttaaatgtg ctaatgattt ttcacgacca agcaattcga tcgtttcagg
    68941 caattctgga ccatgcattt gtcctgatac agctacacgg attggcatga aaagattttt
    69001 tccttttacg ccagtttctt tttgaacagc tttgatggca gctttgacag ttggagcatc
    69061 tactacttcc atttgttcta gctgtgcttt gaatgcgtgg agaacagtag gaactgtttc
    69121 accagcaagt acttcttttg ctgcttcatt caatacagga tgttcattga aaaatagcgt
    69181 agacagatca acgatttctg cagcatagct cattttaggt tgatacaggc taacgatctt
    69241 cttcacccat tcgattgtct tttcatctgg gttttctgct acacgtccat cagcgattag
    69301 ataaggcaga cacatgtcag taaatgcatc tagatccatt gctttgatgt aatggttgtt
    69361 tacccattgc agttttttcg catcgaaagc tgcgggtgat ttacttaggc gttttggatc
    69421 gaacattttg atcaattctt ggtgttcgaa gatttcgtct tcgcccacag gtgaccagcc
    69481 caataatgca ataaagttga acattgcatc tggtagatag cctaattcgc gatattgttc
    69541 gataaattgc aagattgatt cgtcacgttt gcttagtttt ttacctgttt cactgttgat
    69601 gatcaatgtc atatgtccga aggttggtgg tgtccagcca aatgcttcat agatcatcag
    69661 ttgttttggt gtattggcaa tatggtcatc tccacgtaaa acgtgagtga ttttcatcaa
    69721 atgatcatcg accgcaacag caaaattata agttggcata ccatcgcgtt tttggatgac
    69781 aaagtcgcca ccgatattat cggattcgaa cgaaatagcg cctttcacca tatctgtaaa
    69841 tgcgtattct gtattgcgag gcacacggaa acgtacaaca gattctagac cttgtgcttc
    69901 tttttctgct tgttcttcag gtgtcaagtt cgcacatgtt cctgcgtaat gaggcatttc
    69961 tcctcttgcg cgttgtgctt cccgttctgc ttctaattct tcttctgtgc aatagcattt
    70021 gtatgctcga ttgctagcaa gcagctgatc aatcagcggt tgatagattt ctttacgttc
    70081 agattgacgg taagggccgt attctccagg attttcagga gattcatccc aattcattcc
    70141 caaccaagca aggttttcca actggctttt ttcgccatct tcgatattac gtttttggtc
    70201 agtatcctca atacgaataa taaagtctcc atcattgtgg cgggcaaaca aataattgaa
    70261 taatgcagtt cttgcatttc caatatgcag gtgcccagtt ggacttggtg catagcgtac
    70321 gcgtactttc gtcattttct tcctcttctt tcaaactaga ctttgactaa cagcaaaaat
    70381 tccgtcagtt acggcttctc aaatatcatt tatttaaagc cactcagcac aaattttacc
    70441 ttttctaagg taaatagtaa agccaactgg ttgaatttcc ttaccagttg gctgaatttc
    70501 ttttttattt tgattgttta gtctttttct ctgtctcttt tttattgtcg attcctcgtc
    70561 ctgcatgtgc aggtttagca aagatcatgc gtccagcagc tgtttgaagg gcacttgtta
    70621 caacgacttc gatacgttca ttcatgtaat gctgtccatc ttctacgaca accatcgtgc
    70681 cgtcatcaag gtaggctact ccttgttggc gttctgtccc ggcttttact actagaacat
    70741 tcatcgtttc accagggatg acgactggtt ttacggcatt tgccaacgca ttgatattaa
    70801 gaacaggtac attttggaat tcagagactt tattcaagtt atagtcattg gttacaatga
    70861 caccatctaa aagcttagcc aatttgatca atttgctgtc tacttcactg atatcttcaa
    70921 agtctccatc gtacatttcg acagatatac cctcttcttt ttgcaaggca tttaggatat
    70981 ctaatccgcg acgaccacgc acacgtttca aactatcccc tgagtccgcg atgtattgta
    71041 gttcgtaaag aacaaaatta gggatcatca aggttccttc taagaatcca gtctttgtaa
    71101 tatcataaat ccgaccgtca atgatcacgc ttgtatccaa gattttatat ttatggaaat
    71161 tttctcctga ttttcgttct aacagttgac tctcaacttg actagctgtt tcttgctcga
    71221 tttttttgct tcgtgaacca aaaactttct tccattcttc gattcgtgtc gttcccattc
    71281 tcaatcccag atagcctaag atgatcatca gtaaaatcgg caatacacta ttgacgaatg
    71341 gaatttccat attgtaaaga ggggtcgaga tcacaacacc taaagtcaat ccgatgatcg
    71401 caccgatcgc accgaagaga agataagtca agctgacctc acttagtttt tgttctactt
    71461 gtttgataat agctgagata tgtttggcca acagcaaaga taataaaaag aaaataagtg
    71521 cgcctaacag gctgttggta aatgtattat tcagccatcc gtttgactgt tgaccgattg
    71581 cctgccaagc tgagggtaaa aatgaaatac ccaagcttgc gccgacaatg atcatcagca
    71641 aagtaatgac acgtttttgc atcgtttttc ctccgtttcg acaatcattt attagttagc
    71701 ggaacacacg cttcaacgtt tcagccaaag ttgctactcc aactatctcg attccttttg
    71761 gcgcttccca gttacctaag ttgtttttag gtaggtatac ttttgtgaag ccgagttttt
    71821 ggacttcttt gactcgttgt tcaatatgac tgacacgtct gatttctcca gtcagtccaa
    71881 tttcaccgat gaaacattcc gttggctgtg ttcctttttc tttatagctt gaagcaatac
    71941 tgacagcgat agctaaatca atcgctggtt catttaattt taccccacct gcagctttta
    72001 agtaagcatc ttgattctga agaagcaacc cagctctttt ttccagcact gccatgatca
    72061 gagatacccg attgaaatca agacctgttg ttgttcgttt ggcatttccg aacatagtag
    72121 gtgtgactaa tgcttggatc tctaccaaaa ttggacgtgt cccttccatt gccacaacga
    72181 tagcagagcc agttgcaccg tctaggcgtt cttctaagaa aatctgtgaa ggattagcga
    72241 cttcttccaa tccatgctca tgcatttcaa aaatcccaat ctcattcgtt gagccaaatc
    72301 ggttttttac tgctcgcaaa atccgaaaac tatgatgttg ttcaccttca aaatataaca
    72361 ccgtatccac catatgttct aacattcttg ggccggcgat cgagccttct ttggtaacat
    72421 gaccgacgat aaaaatggct atcccatttg ttttagcggt tttgagtagt tctgcagtgg
    72481 tttctcttac ttggctgaca cttcctgcca cacttgtgat gtctggctgc gtcatcgttt
    72541 ggatggaatc aatgataaca taatcagggg agatatgttc gatggcacga ctgatttcgt
    72601 tcatatctgt ttcagcgtat agataaaatt ctgtgttgat acttccgagg cgttccgcgc
    72661 gtaattttat ctgctctgcg ctttcttcgc cggatacata taagactttt ccgcctatgg
    72721 ctgctaattg ctgagaaact tgtaacagca aagtggattt cccgatacct ggatcgccac
    72781 caatcaagac catagaccct ggcaccacac ctccgcctaa cacacggttc agttcttcta
    72841 attttgtttt gatccgaggt tcttttttag ggacgacgtc tgcgattttt gttggttttg
    72901 ctttttctcc tgtcaaactt gtccgagttc tgcggtccgt tgtatcttgt tcgatttctt
    72961 cgaccatcgt attccatttt ccgcaatttg ggcagcgtcc caagaactta ggcgacacat
    73021 acccgcaatt ttggcaaaca aattgtactt ttgatttttt tgccattgta ccgcctcctt
    73081 ttatcctaat catgagacat taaatcaagc ttaagtatct ttaacgtttt atttactgga
    73141 agaaccaaat ccgccttgac gttgagcagt cgtttgatct tgatctgctt ttaaaaacgg
    73201 caaaaagatc ccttggccga tacgctcgcc ttttttgatc actacatcct tgaaaccaaa
    73261 gttggtgaat tggaacatga tatggccttc atttgcctca ttattgtaat aatcactgtc
    73321 aatcacacct actccgtttg tcaacactaa aaaccgcttc aacggattgc ttgaacggtt
    73381 agcaagctgc aaaaattcat cttctcccat gtaagctttg attccagtag gcacgagggt
    73441 tggtttcaat tgtttttcat tttctgatac atcttttttc aatccatctg cgactgtttc
    73501 caaccccttt ttccagcctg ctttccagat acttggaatc acgacctctt ctgctgcttc
    73561 aaaatcatag ccagcagcat gatgtgtagc gcgctgcggt agattgatat tttgatcttt
    73621 atatttagaa ataacttcaa atcctctttt tctcaccggt tttcctccct aaataaacta
    73681 ctcctattct aacatatccg aggctgtaaa agactttctt tacaaattct tcaagaaatt
    73741 ctgctatctt aaagtaaacc attaattcgt tctttttcca aacgactgca agttgtacaa
    73801 aaaaactatt ttttacatga ctttactcgt caaaacaagt agaatagaac gaaattaatg
    73861 agtggatatt tgattgaggt gtcacatgcg taaagaaatt aaaaatgcaa aacgaattat
    73921 tgtcaaagta ggaacaagta cattgattta tcccaatgga aatatcaatc ttgcggcaat
    73981 cgatcagtta gcttttgtcc tttctgatct tagaaatcgg ggaaaggaag tgatcttagt
    74041 ctcatcagga gctgtaggcg ttggtatgca taaattacat ttgtcagaac gtccgacttt
    74101 gattcctgaa cagcaagctg cggcggctgt tggtcaagcc gaactgatga atatctataa
    74161 tcaaagattc agtgtatata atcaacagac ggcacagatc ctcgtgacaa gagacgtggt
    74221 agattatcct gaaagtcgta aaaatgtgag gaatacattg gaacagcttt tgattatgga
    74281 cacgatccct atcgtgaacg aaaacgatac tgtagcagtg gatgaattag atcatctgac
    74341 aaaatttggc gataatgatc agctgtcagc catcatggca aaacttgtcg cggcagacct
    74401 tttgatcgtc ctttctgata tcgacgggtt ttattcagat aatccgacta caaatcccga
    74461 ggctcagatg tatcgctata tcgataaaat cgatgaaaag attctggcac aagcaggtgg
    74521 tgctggtagc acatttggaa caggcggtat gcagagtaaa ttaaaagccg cttctaggat
    74581 atttgaagca aacagcgcaa tgattttagc aaatggaaaa aatccggcaa ttatttttga
    74641 tattcttgca ggaaaagaag taggtacttt atttaaggag gacttgaaat gactgacttg
    74701 atgcaattag gacaacaggc aaaagatagc gcgcaacaac tagcacttat gcctacaaac
    74761 cgcaaaaatg agctacttgt tcaaatggca caaaccatca aaaagaatca acaagcgatc
    74821 attgaagcaa atcataaaga cttagaaaaa gcagtcgaaa acaatatcag tgaaacgatg
    74881 caagaccgct tgcagctgac acctgagcga atcaatgcca tggctacaga gatcgaaaaa
    74941 attgcttcgt tagacgatcc tattggaaaa gtagatgaga tgtggacgaa caccgacgga
    75001 ttgagaatcg gcaaaaagcg cgtgccacta ggcgtgatcg gcatcattta cgaatctcgt
    75061 ccaaatgtca cgacagatgc agctagtcta gcattcaaat cagggaatgc cgttatcctt
    75121 cgcggtggca aagaagcatt cttctcgaat caattattag tccaattatt gcagcaagta
    75181 ctggtcagtg ctggcgaatc tccttatgcc atccagtttg tcgatgatac ttctcatgaa
    75241 actgcaggac agttgatgcg attgacagaa tacttagatg tgctgattcc tcgaggcgga
    75301 gcaaaattaa tccaacgagt caaagaacag gcaacggtac ctatcattga aacaggtaca
    75361 ggaaataatc acgtatatat cgataaagaa gcacagttat ctatggcagt gaacatcatc
    75421 gtcaatgcaa aagcttcccg tccatctgtc tgtaacgcgg cagaaacatt gctgatccat
    75481 tctgagatcg cacctttttt cttgccagct attgaaaaag aactggtaga acacggggtc
    75541 tctttacgtg cggatgctcg tgcgttagaa tatctagaga cggctgcttt agcagaagac
    75601 acagattggg atactgaata tctagattat atccttgctg tcaaagtggt cgattctttg
    75661 gatgaagcaa ttgcacatat caatcggcat aatactaaac attcagaaac gatcgtgaca
    75721 gatagttatg cggctagtca acgtttctta aatgaagtgg acgcggcggc tgtctatgtc
    75781 aatgcctcca ctcgttttac agacggatcg gtatttggtt ttggtgcaga gattggtatc
    75841 tctactcaaa agttgcatgc ccgtggtcca atggggttaa atgaattaac ttccacaaaa
    75901 tatatcattt atggcgatgg acaaatccgc gaataatgtt tcgtgtgaaa cattattccc
    75961 gccttttgcc ttttttctgt aaaggttcta tccaactaga aaggtgtcaa tatgtttgaa
    76021 attttaatga ttttttttat ttaccttatt tctctgaata tcgctgcttt tctgggagtc
    76081 ggtattttat cccttttctt tcaattcaaa aaacgaagca tagggagtca acgggaaaga
    76141 tggtcgcagt attttgacaa aatcgggcaa aaagggctgg ttacacgctt gcacattagt
    76201 tacatggttg cattatgctt attagcgaca atcaattact attctttttt cgatcattca
    76261 atcgcttata cgattacgct actgattgct gggatttttc acttatctta taaatatcag
    76321 ctgaataaaa accacttgaa tcggactttc cgctaaaaaa tatccgtacg ataagtagag
    76381 tgccttgaag atcggcaaaa aactgccgga ctttttagag gctgtgacaa agattttgtc
    76441 acagcctctt tttccatata aacggcgttc gcctaccagc tgcctggtaa atctgtttcc
    76501 tcttcgttaa gaagcgaaaa tccgtcaaat gtttcgccat cataataacg aagcagctca
    76561 tccatggaca agttcatcgt tttttctttt tcttcttccg ctgattcagc cagtagctgt
    76621 tcccgataca cctgatacat accagctatt ttttgctgca tcgcttttct agtttcacct
    76681 tccgcattga ttattggcaa ttctttgata tagccacgat aaatcaaccg atcatctgaa
    76741 aattcaatgg gtaattcaac gagtgtcaac ccgacttcct gaaaatattc ttccagcatc
    76801 tattatcccc ccattctttt tcttaaatga attttagctt acctactgaa aattttccag
    76861 tttcattatt ttgagaaaaa aagggaatgt gacaaaaatc gtgtcacatc ccctttttcc
    76921 gaataaatgg tgttcttctg ccaactcctc aatcataagt cacaatattc aacaaacatg
    76981 agaaactgtt ttttgaaatt gttccttgtg attcagagtt aaacgacagt ctcacaacct
    77041 cttggtaaac ggcgttcaat cagctgaccc tcggtattag ctcaaaaaac ggtaaaatat
    77101 gagaagctat ttttccgttt tgtttctcta atacttagga taaaacgctg attttacaac
    77161 ctctgttaaa cggtgttcaa tcagctgtcc ttcggtatta gctcaaaaaa cggcaaaata
    77221 tgaggaacta tttttccgtt tgtttctcta atactcagga caaaacgctg attttacaac
    77281 ctcctgataa acggtgttca aaagcttgca tctgcggtaa taagcccaac caaacgaaaa
    77341 atatgagaag ctatttttgt ttggctgttc ttatgacttg atgcaggaca gctttttcac
    77401 aacctctgat ttcttattct tcttcatttg tagcaaaata attacggtga taaatgatac
    77461 aacatacact gataagaaca atggcacctg caaaataaaa cgcatagctt tcaccaatat
    77521 gttcaccaat cacacctgca ataaatggtg ctaaagctcc accgaaaaag cggacaaaac
    77581 tatagctgga cgaagcaacg tttcgttgca gacctggtat ttccatagca atcgtcgtca
    77641 acagtgtatt gataattcct tggaaaaatc ctgctaacac aatgctgatc ggtacaagga
    77701 ctggcataga agttccagct cctaataaca gcaaacaaat aatgaataag acaaatgctg
    77761 ctaaaattgt ccgataagtc gtaaacttcc gctcaagagc cggtgcaacg aaaatcgaag
    77821 caatagccag aagcacaccc cagccaaaaa atacaaaacc gacttctaat tcagaatagc
    77881 caatcaataa aaatggcgca tacgcaagta aagtaaagaa tccgaaatta tataacaatg
    77941 cgattagtcc aactgaacga agtttatgat tgcttaacgc tttgacacct tcccaaaggc
    78001 ctacttttct ctttggtttc tcagtttcag gtaaaagtac cgctagggct ataaaaccgc
    78061 aaaacatcaa aaatgctacc ccaaaaaatg gataacgcca tgaaccactg cccaagaccc
    78121 caccgactag agggccaaca gccattccgc agccaagtgc tgcttcatac atcatgattg
    78181 ctttttctgt gttaccaact aaaatactga cgatcgttgc taaagcagta gagacaaata
    78241 aagcattccc gacaccccag ccggcacgta acccaatcag cattccgata ttcccagata
    78301 atccgccaag accagcgaat acaacgatga taaacaatcc tgtcatcaat gtttttttca
    78361 aaccgatccg actagataag aaacctgtaa atagcatcac cacaccagtg actaacatgt
    78421 aactagtaaa taacagggtc gtttcagctg gcgtagcgtt taactgtgca gcaatcgttt
    78481 ttaagattgg gtctactaga cccactccca taaaagcgat catacaggta gctgctacaa
    78541 ctagcgctga acttttcccc tttgtttctt catttgtttg ttcttgtata tctatttgat
    78601 tctccaatat ttccatccct ttctctctat tagcatcata catgatttat tttacatgtc
    78661 aatatgcaca tgtaaaacgt ggaaacttta ctgtgatata ataaagaaaa taagacgaga
    78721 ggtaggattt ttatgaatac tttagcctat atattattat gcgtgctttt atccaaacca
    78781 cgttctgggt atgaattgaa gcagctgatc acaatttttt gggaagccca tcacagtcag
    78841 atctatacaa cgcttgccaa gttagaccag cagggttatg tgaccgtttt ggacaaagac
    78901 gagcactctc aaaaaaagat ctaccacttg actgatgaag gacgtgctct tgtagaggaa
    78961 tggatcaaag aggaaactcc cgctcctacg caaaaagatg aatttctagc aaaaatctat
    79021 gctttttcga cgctcgataa aacaacagct actggactgc tattcactag agaacaacag
    79081 ctgaacaaaa tcatgcaaaa aaatcaaaaa aaattgaatg acctctcgct agacaagaaa
    79141 caagaatttg gacgttatgt tgtgatccag cgcagaatta tgctttgtca gcaggaactc
    79201 caatggtgtc agcaagtaag agaacaaatg cagcagtttt ttgagtaagg gataaacaga
    79261 acctttctag aaatatctat tgaagagcaa attatcttca ataaaagagc aatacagttg
    79321 gaaaagaaaa agcgatccga acgacaggcc aaaacctatc gttcggatca ctatttttct
    79381 agaactgcat tcaagagact atgacaaaaa gcatgccaag gccttttttc aaataacagt
    79441 gttccatcat tcattacttt gaattgcttt tagcacactt cttgaaagcg ggccaacgat
    79501 gatcaactgc aacggtaaag cggcaatcac attcattttc caagcattca tataagcaga
    79561 gaataattga tcaggtattc cttgttccat gatcacaccg aataaagaca tacatgtcac
    79621 cattcctaaa accatcaagc atgaaatagt taagatcatt tggaccttgc tttctggatt
    79681 tactggtagt ttaaaggcaa tttttttcgc cagcacaccg atgatgaaga catccaaaat
    79741 gaaagccacc acgaatccag gcactagacc aatcaacaaa cttgataaat gaagatcttg
    79801 atgcagccat aaattatacg cgctcatccc caagaccatc aaaaaacaca tcattgttgt
    79861 aaaaattatt ccttcttttt tatttgtagg caatctgtac cactccttta aatttgtcac
    79921 gaaaatcatc gtagcacatt taaaaatttc tcgtaagtac tttttttatt ttctgccctt
    79981 ttatttcata aacttcatca gccgtacgct cgacaaagta agaatcatga gaagtaaaca
    80041 aaatcgttcc cggatatatt tgcattagct tctctaaagc ttcgatcgtt gccatatcaa
    80101 taaaattagt cggttcatct aacagcaaca cattacttgg cttagtaaaa agcaaagcaa
    80161 tcgtcagacg ggtcgcttct cccccactta atgtacataa cggtttcgtc aaagtttcct
    80221 gagcaaaccc taagtgattc agcaaactac ggacaagcga ttctgaaaac tctgtttgtc
    80281 tcatgacaaa acgaatgatg gattcttcag atgtcatttg ataacccttt tgctgatata
    80341 cttgaaagct tactttcgga gagagaagga ttccttctcc ttgtttgcgg atatgttcta
    80401 ataaggttgt cttacctgaa ccattttcac cgacaatcgc gattttttta cctaaagaaa
    80461 actgaaaatc accatttacc agtaatgttc tcccacttct ttccaattgg acattttgtc
    80521 ccatgatggg atagcggctg tggagttcaa gagattttgg tactggaaaa cggatttgtt
    80581 tcatctgctg cggcttggtt gtttctccta ttttttggag tcttctctct aatgttttcg
    80641 cattcttttg gatggctttt tgtacactgt ctttttgttt agaggaagac aaccgatcca
    80701 gtctgatttg ttgctttttc tttttactgg acactttttc caacttttcc gcttgttttt
    80761 tcttttcttg gatcgatttt tccaatcgtt ttttctcttt catgatcgac tctgcttctc
    80821 gtagctgagt ctttttctcc aattcctttt gagtgagata ggcactataa ttccccgaaa
    80881 actctcggat ttctccgtct tttacttccc agattttcga tgctagctca tctagaaaat
    80941 agcgatcatg actaacaaaa accagcgtac cataataata acgaagctct tcaatcagtc
    81001 gatcaatact ttttctatct agatgggttg ttggctcgtc tagaagcatc ccttgttcat
    81061 aagaagaaag aagacgtatc aactcgatct ttcgttcttc ccctccgctc catttccgct
    81121 ctgaagtttc ttccagtctt aactggaaat gactggctaa ttccaaattt aggtcctttg
    81181 cttcagcgag ataggttaat tgaggcaggt agttgaaggt gatctctgtt tggattttcc
    81241 catgatcggg aaaaagttca ccggctatta gtttgagcaa agtcgattta ccgacaccat
    81301 tttctcctat gatccctata cgatcttgct gatagacacg aagagaatca atagatagtt
    81361 ctagctggtt gccaaagctg acttgtagat ttgttatatt tactgctaaa ttttccatat
    81421 gttttacact cctatttcat gtaaaacaac aattatcgat actaaaaaag agcagaaaaa
    81481 tctgtttgca aaaaaactgc atcacatctt ttcctactct gaacgaaata actatgacta
    81541 aatcaccaaa tctgtcatag ctttcaccta ttctactgtc gttcgaaaaa atggacatac
    81601 tccatccatg aaattgttgt ttatacatgt cttttgaatt tatttagttg ttattcaaaa
    81661 gttcgaaacg taatttcatc gatgcagtca tttttccgac ctcctttttt attaggttac
    81721 cacacacgat tcaaaaaaag caatagctac tgaatagtaa ctgaaaacct tttattcgaa
    81781 catagtgatc ttttctggtg ctaaacgaac agactcgtaa ccttcttcta ccgcttttcc
    81841 tattgataaa ataataactg gaacataacg gtctttatcc aaaccaaatg cctctgccaa
    81901 ctgatctgct tcgaatccac caattggatt tgtttcatag ccatgatcac gcgcaactaa
    81961 catgaattgc atagccgcta agctagaatc gactttgact acatcgttca tttcttctct
    82021 tgaaaaactc ttgtaataag gaatgatcgc agctaattgt tgatctctga cttcttttgg
    82081 catcttccct gattcgtaag cttggttgta gatttcttcg ccatattcgt aacattgcat
    82141 atcgccaaaa atcatgacca ttgcagaaga agtatcattt tgtctcgtat tgaaacggat
    82201 caaaggacgc aagatttctt ttgcttcctc gctttcagcg accacaaagc gccatggttt
    82261 catattgaca gaagaaggtg cagtcgtcgc ttctttgatc atttccagca tttcttcgtg
    82321 tgggattttg tatgtttcat catatacgcg aacggatctt ctaccagtca taattgttga
    82381 aaaatcattt ttttgttcca ttattattaa cctctttcgt ttctgttgtt atattgcaaa
    82441 gttgggtatt caatttatct aaaagtgtta gaaattgttt catttctaca tcgctgaaag
    82501 ccggggatag cacggaacct gtatctttat ggtccatttc acaatgcttg attttcttct
    82561 gggctttttc tgttagcgct acatacactt cccggttgtt ctgaggattt ctctgacgcg
    82621 aaacatagcc atttgcttcc aataccttca gatgacgagt aactgctcct tgatcaatct
    82681 gcaacttttc ttgcaaactg ctttgtagca atggattttc ttctttcagt ctcatcatga
    82741 tctcgtatct agtaagactt ataccgatat tttcttcaaa gatctggacg attttttgat
    82801 cggctaattt gatttgatat aaaaggtgac taatctcttc taaagacata atttcctcct
    82861 ttctaataat tgactcgtca acgattgact tgtcaataat agaataaaaa aagaaaaaag
    82921 tcaaagaatc agctgtgtaa taaaaaaaac gctctcaata gaaaagtata gagaaaagaa
    82981 attttttgta gaatgaacgg aaaaaatgat aaagaaagca caacgctaaa accaagatta
    83041 ttgattctta tcaaactata gaagatagct cttctgcagt agataatttt acaccgccaa
    83101 aaatttccga aatcgccttt tgcaagaatc ccgtcgaata ttagaaattg gatcagtatg
    83161 cttaaacagt aataaaaaag ttatgacaag atgttgtcac aacctttttt gagtaaacgg
    83221 tgttcaaaaa cttgcatctg cggtaataag ttcaaccaaa caaaaaacat gaaaatctgt
    83281 ttttgtttgg ctgttcttac tacttgatgc agggcagctt tttcactacc tctttttaat
    83341 aaccataaaa tcctattcct tcatatctcc agcccatcgg tgttagccaa tctctttcct
    83401 tagcactcaa ggtatagtga tgggtaccag ttttttcatt tggattaaat agtcgataaa
    83461 ttggcttgga accaccagaa taaaaactta gaccttcata acgccaaccg atttttgtta
    83521 aagagttgat ctcagattgt gccattgtgt aatgatggtc tccactgtaa ggattgtata
    83581 aacggtagac aggttgtccg ctgtttggtg cttgccaagc aatcccttca tattcccagc
    83641 ctacagttgg cagataattt ttttctccgg cattcaatgt atataaatgt tctcctgtat
    83701 gacggttata cagacgatac atcggagaag ttgaagttgg tgctgtatca tactgtgtca
    83761 gattataggt ttcgattaca ttgttcaatg tagaaccata attaggcgca gtggcataac
    83821 gacctgttag ccaagccgta gcatctttgt aagagttcgt attgcttttc catgcaccgg
    83881 aatagtagta aacgcctggc tgaaaagaag tcgtcttcaa taccgttacg ttatcttgca
    83941 atgattcttt gtacgaagga tattttctaa aagcggcatt caccgtaatc cattgcccat
    84001 tcacatattc cgaagtaggc atcgtaacag attggccatt atagcttcct ttaatcccaa
    84061 atagattgta attaggggca gaagccaaag tactgttccc ccagccactt tcaacgatgg
    84121 cttgagcaat catgactgat gcatataagt cattggcact tgccaaactt tgtgcagttg
    84181 aggcaatact atcaatgaac gcttgttgag aattagcaga tgagcgagca taaaaagcat
    84241 agccagtata gttttcgaat tcagacaaat ctgcttgatg ggtatgtgtc aatgtttgcc
    84301 catctgtcaa ttggttcgtg atttcttctg cattctctgg attgttctga gcttctgctg
    84361 atgtactgtc ttcggtttgt gtttgttctg ttgtttcagt tgttggttct tcggttgaaa
    84421 ttgtattttc tgagacagta gctgactgca aagtactttc tgtatcgttt tcaattcctg
    84481 tttctgaaga actcgatgtc tgagttgcga cagtttctgt cgattcttca gctagcactg
    84541 ttgtgttggc aactgctaga tttcctaaaa gaataacgct aaataataaa ttctttgctt
    84601 tcatcttttt tctcctttga attatgaaac ttttcctaaa taacagtgta actaaaaaag
    84661 taaaagcaag tcaatgtcca tatttaaata acatttgttt ctttttaata aatttgcttt
    84721 ttctgcataa aaagacgtaa atgaagaata ttcttctcca ttcacgcctt aaattgatgg
    84781 tcgtttgaat aactatttaa caagtaattc tctctggata ttatccgtta tggattatac
    84841 ttcctgtgtc gtcggacgga taatcatgtt gctgacagac atccggtctg gcgtttctat
    84901 cgcaaatgct acagcagaag ctatatcttc agatgttaat ccccattctt tttgcgcttc
    84961 atgaagttca agagcagcct ttttgtcgct gatggtttga tataattcag tttgaacagc
    85021 tcctggagag atgatcgtag atttgatgtt attttgccgt tgctcttgac gtaatccttc
    85081 catgattgca cggacagcaa attttgttcc gcaataaacg gcggaatcag gataaacaac
    85141 atgtcccgcc acagaatcag ttgaaatgat atgcccagat ttttgttttt ccatgattgg
    85201 aagtacggcg gaaattccat ttaacacacc catgatgttg atatcaagca tattttgcca
    85261 ttcatcacgg tgaccttcaa ctaatggagc ggttggcatg attcctgcat tattgtacaa
    85321 tacatcgatt cgaccgaact tttccatcgc taaatcgata actgcttgga cttgtgcaaa
    85381 atctcttaca tctgctgttt gaatatatag ctcagcttct ggtaaagatt cttttattgc
    85441 ttttaggcga tcttctcggc gtgcagctat aactagttta gccccttttt ctgctaatag
    85501 cttcgttgtt gcttcaccga ttccgcttga agcgcccatg atgacaatca ctttttctga
    85561 taaatcattc attctattct cctcctgttt tgaaaaatct cttgcttata ctcactatct
    85621 cttatttcat cttactgttc aaataatccg ttcgacaggt ttgacgattt tggcagggat
    85681 tcctgcaacg atggtattgt ctgggacatc tttcgtaaca gtgcattcgc tgcaacaatg
    85741 gcattctcgc caatcgttac gcctggcaga atagttgctc ctgctccgat ccaagcattt
    85801 ttcttgatgt ggattggctt tactgacaag cctcttcgtt cttttggatc ggttagatgg
    85861 ttcaccgtga tgaggttgac acgaggacca atcaatactg aatcttctaa acagatgcca
    85921 cctaagtcaa cgaacatcac ttgacgattg ataaagacat ttttccctat actaatatgt
    85981 ttgccgaaat ctgtataaaa aggcagcaga acagtcaccg tatcatctac tttgtcacta
    86041 atgatatttt ctagccgttt gcggatatct gctggttcat ggtacccagt attcagcgct
    86101 gctaaaggct cttcgttttc cgcttggatc tgatggatca cttcaaagag cggatccgtt
    86161 actcgaatct ctttattgac gatttgtttt tcgatgaggc ttgtcatttt gctccctcct
    86221 tttatcctta ctttataacg gacggcttat gatgtctaat acttataaaa catacttaac
    86281 tattcctttt aactatactt agtggtatgc ttttttaaaa acaggaggga aaaaatggat
    86341 attcgtatac tgacgtattt tttagcagta gctagagaaa aaaatatcag ccgagcagca
    86401 aaatctctgc atatcacgca acctactctc tcaaaacaac tgaaagattt agaagaagaa
    86461 cttggtgtga cacttttcac tcgaggctcc cgagagatac agttgacgga ttctggtcga
    86521 tatctttatt caaaaggaaa agaaatcttg tcgttggtag aaaaaacggc gaacaattta
    86581 acaaatgacg caatcatctc tggagaaatc tacattggcg gaggcgaaac taaagcaatc
    86641 tctttcattt ctgatagcct tcattcgttg atcaaggcgc atcctgatat ccagtttcat
    86701 ttatatagcg ggaatgcaga tgatatcata gacaaactgg atagcggatt acttgatttt
    86761 ggactcgtaa tcgaaccgac aaacaaacaa aaataccagt catttcgact gccagcccaa
    86821 gatcgttggg gacttttgat acatcggaat catcctttag caaagcaaac agtcatccat
    86881 ccgcaggaac tgaaaactgt tcctttgatc atttcccggc aaacgtcagt ggacagtcaa
    86941 ctagctgaat ggctaggttt cagcatagat cagttatcga tcgtcggaac atataactta
    87001 ttgtacaatg cttctttgat ggtcgataac gggacttttg gtgcattagc tctagatggg
    87061 atcatcaata cttatgggac ggaaaaagta ttcatcccac tcgatccgcc attacaagct
    87121 tcactgaatc tcatttggaa aaaagaccag cctctttctc ctgctgctcg taaattttta
    87181 gatgtcctaa cggagaaata cagttaagta gagttttttt ccttcgcttt cagcttttta
    87241 acaacgaata agttaagcaa aagatccaat gattaaataa cataagtttc ttaatttgtt
    87301 ccttcctttt atttctgata attccagtaa attactataa ttataaaaaa agaattgaca
    87361 aaacataact gtctatataa aattgattac gttaacaata attcgtttca tgtgactaga
    87421 tcatactagg catgaaaaat ctctgaggaa catggtttcc ctaggaggtt tttcgtgtct
    87481 atttttgttt ggccgcaatc aactggtcta gcaaacgtga aaaccaaatc ttcaaaaagg
    87541 gtgcgcgcat gaaaatcaaa aagatcttta atcaaaatgc agtattggta gatgacggtg
    87601 gacaggaaaa agtagctatt ggaaaaggga ttggatttga cagaaaaaga aatgatttga
    87661 tttttgatcg tgatgtcgaa cggatatttg tcatggaacc agaaggtcaa atcaagttgc
    87721 aaaatttact taatcagatt gatgaacgct ttttatttgc agcagaaacg atcattgatc
    87781 atgcggaaac tgttttgatg gaaaagttga atgaacatgt attgatcgct ttgaccgacc
    87841 acattgcttt ttctgcggag aacattaata acggtatcgt catccgcaac aaacttttaa
    87901 gagaaatcga agtgctttac agtgatgaat tcagtattgc tcaatgggca gtagagtatc
    87961 tgacaaaaga gcttgggatt ccatatactt atgacgaagc aggatatatc gcaatccata
    88021 ttcacagcgg acgaagcggt cggaacaata accatcgcag tatccgtgaa gtgacgattg
    88081 tatctgacat catccattta gtggaatcag aattggaaac agatatccat tcagaaccat
    88141 tttcactgaa ttattcaaga ctggtgaatc atttacgact gcttctacaa cgaacccatg
    88201 cgcaacaata tgctgtttta gacacggaaa tcgtagaaat ggtcaaacga aaatacccag
    88261 aaagctataa gatttctaaa aaaatccgtg tgttgctgac caaagaatac cagctcgcga
    88321 tcacgaaaga agaactgggt tacttggcca tccatatcga gcgtttaaga agcgcaatcg
    88381 tacaaacaaa tgttaacaat catgaggagg aaaaaaattg aaagcatatc tacaacgaat
    88441 gggacgttcc cttcaacttc cagttgcggt cttacctgct gctgctttac tagtagggat
    88501 cggaaactgg tgggcatctt atagtaacga cattgtcgct cattttttac aagctggcgg
    88561 taacgcagtt ttaggtcaat tacctttgct gtttgccgtt gggttagcac tagggatgtc
    88621 agtggacaaa tcaggtgctg ctgccttggc aggtttggtc gcatttgaag ttccagtaaa
    88681 tgttttaaaa actgattcag tagctacttt attgaatatc aaaccagaat cagttgatcc
    88741 atcttttgct cagatcagta acgtatttat tggtattatt gccggattga ttgcggcagc
    88801 tctttataat cgtttccacg aagcaaaatt accgatggct ttatctttct ttagcggcaa
    88861 gcgtctagtg ccaatcttgg ctgcctttgt catgttgctg atcagtgcag tgctactgct
    88921 cgtatggcct ccggtttact ctgctttagt ttcttttgct aaattcattt tgagtttagg
    88981 ctggggtggt gcaggtcttt atggcttctt caaccgttta ctgatcccta caggattgca
    89041 tcaagcaatg aactccgtgt tctggtttga cgtagcagga atcaatgata ttggtaaatt
    89101 tttggcttct gaaggaacaa aaggcgtaac aggaatgtat caagctggat ttttcccaat
    89161 catgatgttc gggcttccag caggagcata tgctatttac agaaatgcac gtccagaaaa
    89221 caaagcaaaa acagcttcct tgatgttagc agcagctttt gcttcattct ttactggtgt
    89281 aacagaacca cttgaattct catttatgtt cgttgcttgg ccattatatg tcttgcatgc
    89341 aattttcact gggatttctt tagcagtgag tgcattcttc cattggacag caggatttgc
    89401 ctttagtgcc ggatttgtcg attacttcct atctttgaaa aatccgattg cgaatcagcc
    89461 attgatgttg atcgtccaag gattagtaac agcagtgatt tactactttg gctttaactt
    89521 tgctatcaaa aaattccatc tgatgactcc tggccgcgag gaagcagatc tttcagatga
    89581 cgatactgca acgactaatt cagacaataa atatgcggca caagcaagca aaatctacgc
    89641 tgcattaggc ggagccgaca atgtaacttc gatcgataac tgtactacac gccttcgttt
    89701 gcaagtcaaa gatacaggcc tgatcgatca aaataaaatc aaagccactg gcgtacctgg
    89761 tatgaagatc atcgacggaa aaaatgcgca agtcatcgta ggtacagaag tacaattcgt
    89821 tgcagatgag atggcaaaac tacatggtgg tgctgcaaca cgacctgcac aaacaaacac
    89881 cgtggttaaa acagaaacat ttgccggtgg aacgatcgaa caagatattt acgctatcgc
    89941 aaatgggaaa ctgattccaa tcactgaagt ttcagatgat gtattcgcgg aaaaaatgat
    90001 gggtgacggc tatgctgtat taccagaaaa tggagaaatc ttctctccaa tcgctggtac
    90061 gatcacaaac attttcccaa cgaaacatgc tgtcggaatc caaacagatg ctggtatcga
    90121 agtcttgctt catatgggca tcaacactgt tgacttaaaa ggcgaaccat tcactttata
    90181 tgtaaaagaa ggtcaaaaag tcgcaagagg tcaattgatt gctttagttg atcttgccgc
    90241 tattcaatct gcaggcaaaa acacggatat ggtcgttgta ttcacaaatg gcgacaaagt
    90301 ccaaagctta gaaatcgaac ccgcacgtga tgtaaaagca aatgataaaa tcggctctgt
    90361 ttccaacaaa gcctgagcca tcgtcttccc cagacttttt tatcgataaa caaacaggag
    90421 ctgagacaaa aatcttgtct cagctccttt ttccgaataa aaacggcgtt ctcctgccaa
    90481 ctccgcggta ataagtccag ccaaacgtaa catatgaaga gctatttttt atttgactct
    90541 tcttattact tgatgcagaa cagctttttt acaacctctg ataaacggtg ttcaaaaacc
    90601 agtttatcgg tatgaagtca aatttatgca aaaatgtgga aagccatttt cacaaaattt
    90661 ttcttcatac tcaaaactaa tcggcttttt cacaacctct ttatacatta ttcctgatcc
    90721 aatatttctt ttaatgctgc ttcaagagaa gttaatggtt ttcccaatac tttttctaaa
    90781 tcatcagagg aaatagctaa ctgatccttt ttaatatcat gttggatcgc taaaaacatg
    90841 tcagctgctt cttgagggaa tccggcttct tttagatttt cgacaaacgc tgcatcactt
    90901 gcttcaataa tttctaactt tttacctgtc gcacgctcta atgccttggc taattcttca
    90961 tagtcagtta gattgccgga taattctaag atttctggag agtcactttc tactagtact
    91021 tttgctgcag cttctgcata ttcacgtttc aatgcccaac ctgtttttcc atttcctgca
    91081 gcataaacga attgaccagt ttttaaagcc tgcccaatga taggtaattc attctctaca
    91141 taccaattat tccgtaaaaa tgtatgggaa atacctgaat ccttaatcat tttttcagtg
    91201 aattggtgat ctggcgctaa tacactgatc gagtgatcgg catctgcaaa acttgtataa
    91261 gcgatataag ataccccagc tgcttgtgct gccttcacta cattcttatg ttcggcttga
    91321 cgatttcctg gaacgccaga gacaaaaagc aatcgatcta ttcctttcaa cgcttctttc
    91381 attgagtcta aatcatcata atcaccaata cgtatattaa atccagcttc tttcagtgct
    91441 tctccttttt cttcactacg tactagtaca tagatttcat tttgtggaac catctttttt
    91501 aagaattcca gcgcataacc accaaaacct ccagttgctc ctgttactaa ataatgcata
    91561 tttgccactc cctattcttt ttatttgtta agatttacag ctgtacattt ataaattaca
    91621 cctttatcat aaagagtgtg gtaagataag tcaagcaaaa aaaatgaaag gatgacaact
    91681 gatgaagtat tctctgcaat ttagtgatgc aatccatgta cttgcctata ttgaaatgtt
    91741 tcaagatacc cgatcacttt ctagtgagat gattgccagt agtatcgaaa caaatgcagc
    91801 aaatgtacgt aaaatcatga gtcagctcaa aaaatccggt ttgatcttta caaaaaccgg
    91861 aaaaccgcag ccttcccttg ccaaaagtcc agatcagatt tctttactag atgtctatcg
    91921 cagcatcgaa ggtaatacac agcttatcca agtcgatcca aaaacaaatc caaattgcgt
    91981 cgttggtgcg aatatccaag aagctttaga agaaagctac gctcggctgc agaaaaaggc
    92041 agaagaagaa atggctaaga tcacacttga tagtctgata cataaaatag ccgttttgga
    92101 aaaagaaaaa agaccagaaa acacaggcct cgtagagaga tttttataat tctctctact
    92161 gtgcctgcat ttttctgatc ggaatcgtct acataaattc ttttattctc cgggggacaa
    92221 aagccttgtc atgccccttt ttcctctcta atgctcaggt caaagcgctg aagtcatgac
    92281 gtcttgataa acggtgttca agtagctgac cctcggcatt agttctgaaa aatgcaaaat
    92341 atggaaagct atttcccatt tttcctctct aatgctcagg tcaaagcgct actttcacga
    92401 cctctatatt atcatggttt attgactacc ggtatccaga tttctcccat atatttcccg
    92461 ttggtaactc ccatttcgac tacagcattt ggacccccaa cataggcgta atcttctact
    92521 tctcttaaaa catgcccaaa ggccgtattc gtcaaatcat ttcttaattc ttcaggcgat
    92581 tctgctaaag cgattacaac aagatacgtt ccttttggga atgggatgat tcgggtagtg
    92641 tttggaacat cctgactcgt ttgaacaccg acatagtaca tcattttccc gttatagggt
    92701 tcgttgactg taaagaaata atgattttca gcagcttgtt tcaatgcatc tattgttcca
    92761 ttaccgataa ctttttgctc aaatgcttgt ttctctgcag ccaaaccgac aaaatccgta
    92821 ttgctactct tcaattccac accaataccc gtcaccgtaa aatctggttt ttcttcgatc
    92881 ctatactttg ccatttctcc caccctttct attatattaa gttcatctta cataaaatta
    92941 aagggaaatg acacgattac gctctttttt ctagggatat ttttattttt taactatttt
    93001 tttgctttca ctgcaatcca aatctcactg taaacattac tgaaatcctc tccccatttt
    93061 gtaaaagaga tctcgggagc ttcgactaac tcataatctg aagaaggaag ccattctgca
    93121 tagattttcc cccaagtatc ttgtaaagtt tggggaaatg gccctttatt aggaaaaatg
    93181 gcccaagtgt gttcttctat agcaagcatt tctaaatcag cgtacggatt ttcttttgtc
    93241 gtagcaaagc caatcatgtg catcaaactg cccttttctt ccaagcgggc tccatcaaat
    93301 ccataagaaa cgttgattac ttgatttgga tataaatctc ctaacttatg catctcttcc
    93361 ctttgagtct ccgtgatcgt ctgcgctaac ttttgtattt cttgattgac tccttcaaat
    93421 ttgacaggaa cttttttcgc cacacctgcc acataaaact gttctttctt ttcgatacga
    93481 aattccatcg attttcctcc tctgacatca ataaaaaatg tcaagcgtgg aaaagatttt
    93541 tgtatgccgt atctgctgat ttctgaaggc agatatcctg accaatcacg aaaagctctt
    93601 gaaaaacctt cgactgtttc atatccatat ctaaacgccg tttctgtcac tcttgatcca
    93661 tctaggagat cgcgattagc caaagccaat ctacggtttt tgatatagtc cattagcgac
    93721 atccctgcaa taaacgtaaa gatgcgtttt aattgatatt cagagattcc tgtattttcg
    93781 gctattattt tataggaaag tccttctgtc aaatgttctt cgatgtaatc gatcaattgg
    93841 ttcagatctt taagcatatt tatcttcctc cttgcttgcc tttttatcat attatcaaag
    93901 aaagtcaatt acttgatgaa tcaggaacca atttagtcag atgtagaaaa tgttgtatgg
    93961 atctaaagaa aattctcaag cagcgtttcg taacggataa ttggtattat ctgcctatct
    94021 atgttttacc tttagaaaaa taaattttgg ggaagacaca ttctaagtaa acaaagcggt
    94081 aaaaatatct tttattccct tagttaatcc gcttactacc tactgtacaa agcaaattgt
    94141 tgttcaaact gctgatcaga acgctgcttg gatttttctt tgcgatacat catacctctt
    94201 ttatcaattg gaacaattat ttatttctaa caaaatcact tcatttttgt cagataaata
    94261 aaaaacaaaa aaagttccat ctttaaaaca cgctattctt ttactttttc tcacattctc
    94321 taagaaaaga aaaggagaat gaatttttga caattcgagc aaaagtgatt atcaaatatg
    94381 taaaatcagc tacttttttc ttaaacgttt ctttatacta aatataaata agtgtttcat
    94441 gaaaggagaa ctagatgaat aattggcttg agtattttcc tgaaaacgtt ttggaacgcg
    94501 gttacagcta tcatctgcac ggttttgtcc gtcatttgaa ctatacaagc aagtacttgt
    94561 ccgcaacagt ttctggcacg gaagattata aggtagtgat cacttgggat gaaaagacca
    94621 acatgacttg tgattgtcta tatgccatag aaggaaaaaa atgcaaacac atggctgctg
    94681 tcttattcgc ttacgaagaa cgtccaataa aaaaatccaa ctactcactt tccgaactct
    94741 cgtcccttgt ttcctccgct agttcctctc ttgtacggga gcttttaaca gagattctta
    94801 tagaacatcc gcaatttatc gaacgcttca aagttaagat gccatttcat gcaataaatt
    94861 attcagataa attaacaact atcatccata aatacgatca tattatcaag aaaaacaaaa
    94921 acaggaaaac agcgaagttc atcatggaga tgcgaaagtt catccaagaa gcagttgaat
    94981 cgctcattca gcaaaatgcc tatcttcccg cattcgaatt gatcaatgaa gtgattgcta
    95041 ctctcgaaac attctattgg gagcctgagg atgaacgaac ccttcttctg atagaggact
    95101 gttattattt atggaaagaa ttattagcag aagcacctca tgcagaaaaa aggcaaatgt
    95161 tctcttggtt cgtctgccaa gtcgatcata ctgacgcctc ttattcaaaa cgctactcca
    95221 taaaaatcct gaaagaagac tttcgggaaa aagaattttc taaccaaaag aaaaaaatag
    95281 ataaaaaaac taaagaaacg gtaaagaaag acgatttcaa tgaaaaatag caagtaaacg
    95341 ataacaacca tcctaaatcc acaagaaata cagcagacct tgctttaaaa acacgaatca
    95401 tgcaaaggat gaagtaaaga agacttaacg cgtacccaat attagtcatt tgactattag
    95461 gtacgcgttt tgttttataa gtcatgacaa atttccttcg ttgctgggta gattttccgg
    95521 cattgttgat agattttatg atacttttct acattttgta agagaggttc cactgcatct
    95581 gtatattgga taaaattctg acaacaactt tcaggtgtgt cgaaccactc taatctttaa
    95641 agataaatat taaaataacg attaataata ttaacagtct ttatttttca ccaccaaaaa
    95701 cctatctttg catatactaa atagacgtat atacacagat aggtttgtct tgtgaaaaat
    95761 aatgattatc cttaccaaaa aatgtttgtt tttctacatg ttcgtactta tcaaaaaata
    95821 tgatccttat tgaaattact ggctatttat attccaacga cttcttgtgt taatttttgt
    95881 tatctcatag taaattatgt ttcattttca cttaatttag ttgtcacgtg ttcgtcaatt
    95941 cgtaaaaacg gtaaatatat aaacacactt gcggctataa ttaatatttg taaaacagct
    96001 gctcgccagt ctccggctgt tgctaaaaat cccgatataa ctggtggcgt agtccaagga
    96061 atttgcacta ctacatgatt aattaatccc acagcagttg caaaatatgc tactgtcaaa
    96121 gaaaaaatcg gtgcaagcac aaagggaata atcagactga tattaaatac aataggcaat
    96181 ccaaaaataa taggttcgct tatattgaat atagcgggag ttaccgataa tttagcaaag
    96241 tccttataat cttttctacg actgaagata aatattgcaa tcaataaagc aatcgtattt
    96301 cccgatccac ccattaccgc aaaggtatct ttaaaggcca tattgataat atgtggaatt
    96361 tgctctttgt ttgcataagc tgccatattt tcttgcatat tctgaatcaa aaacggatct
    96421 aacaatgaac ctgatattac tgcttgatgg ataccaaatc caaaaaatag attagcaata
    96481 gatgtaatta acaaaaatcc tggtaaacca gttgtaattt tacttaatgg ctctgtaact
    96541 atcgtggtaa ttagcttatt tatatccata ttaaacaatt gaatgattat aaatgacaat
    96601 aaagcaaacg aggtaatcgt aataatgatt gggattaata cattaaaaga ctttaaaaca
    96661 gcgggaggaa tatttccgga tagctgaatc tgtagtttct tattattaga aagtttaata
    96721 aataattctg tagccgctaa tccaacaaat atacctacaa acattcctga agctcctgta
    96781 taggatatag gtatcacacc cgtcacttca acagctttat ccgcattttc aggagtaaac
    96841 atcatctgca taggtgttaa tacaacaaat gtcgaaatag caactaatac cgctgcaata
    96901 tgatttgtat aatttctgct tgcacacata tgataagata ctgctgctac aactaataat
    96961 gtagttatgc ttaatgtacc attggcaatt gaagtaccaa tttgttgtaa cgtggtcaat
    97021 gtactagtat cgacaatttc ccccatgaat cctgttggtt ctaagatcac tgaattaata
    97081 agtgtcacaa atcctgctaa catcataaat ggcataaatg tagcaaacgc atctcgcaaa
    97141 gttcttaaat ggatctgatt ccccaactta gtcgcaacta taccaaattt ttcaaataca
    97201 atgtccttcc tgctactttt gcccatattt tttctccttt atgcttattg taatcgatta
    97261 ctttttccac ccaaataata catcacttat caaaaaaagt aaatactttt aattaaatat
    97321 atatatcttt tttagtataa aaaaataagc tctgaaaaaa taattttccc gagcttattt
    97381 ttcaacaaaa acagcttcaa aaacaaagtc ttgatataaa tgtctcgata ccgaatactc
    97441 gaaaggttta ccagacgata gagcaacaac tttgtctatc tcaatcataa attcacctgg
    97501 atctattttt aatatttttg cgtcaaattc attcgcagta tctcctttga tacgtatgtg
    97561 cgaagtcttt atttccagac ccagttcatt tgtgatataa gcatatacag atttctttaa
    97621 atgttttggt tccaagcctg gtatgactgc taatggcatg aatgtttgct caatcgagta
    97681 tggttgacaa tcaataaaac ggcttcgtac aatattatat acatagtctt ttacactgat
    97741 acttaattta tcagctactt ttttcgatgg aagttcaatt gagaaagtat ggatttctga
    97801 atctacgtgt tcttcaccaa ccacgctaaa caaaccttcg tttggaccaa gaactcgtga
    97861 gttatcaatt ggtgttctga cgacttcagt cccataccca cttctacttc gcaaaactcc
    97921 ttcggaaaca agtaagtcta cgcctttctt tactgttaac ttgcttgtat gataaataac
    97981 tgccaattca tctcctgtag gaagtttctc accaatttta tactctccat ttacaatttt
    98041 tttctttata tctaagtaaa tttccatata cttcgcaatt tttttcaatg gtgaccctcc
    98101 aatcaaaaac atatcttcat ctatacttta acataaataa accgataaaa tccacatcaa
    98161 aattatacac acaataaaaa agatatatat atttaaacaa aactatttac tttaagttgt
    98221 aagcggtgtt atcattaagt ggaaacagga ggtaaaaaaa ttatgacaac tatgaaaaag
    98281 ctaacttttc cagatgattt ttggtggggt tccgcttggt cagctgagca agcagaagga
    98341 aaaggagaaa caggaaaagc agagactatc tgggatcgct ggtttaaaga acaaccttac
    98401 cggttttatc aacgaatcga ttcctctatt acaaccgatc acatccatca atataaagaa
    98461 gatatagaac taatgaaaaa aactgggcat aactcctttc gcgtttctat ttcttgggca
    98521 cgaatgtttc caaatggcgg taccggaaaa gtaaatccca aagccatttc tttctatcga
    98581 aaattatttt ccgaaatgaa taataacgga atcaaagtat ttgccaactt atatcatttt
    98641 gacatgcctg ctactcttca agataaagga ggatgggaat cacgtgaagt agttgatgct
    98701 tatgttcatt acgctgaaac ttgttttaaa gaatttggtg acttagtttg tcattggttt
    98761 accttcaatg aaccacttgg gcctatttta ggtacttatc ttgaagattt tcattatcca
    98821 aacctcattg actttaaacg cggagcgcaa gcagcattct tcactatact tgcacacgca
    98881 aaagcaatac aagtatttaa agaactagct cttcctagta aaataggcgt tattctaaat
    98941 ttgagtccta cttttcctcg tagtcaaaat cctgctgatg tgaaagcagc agaaatcgct
    99001 gatttatttt atacgcggag ctttttagat cctatggtta aagggaattt ttctaaaaag
    99061 cttatcctgt tattacaaga atataatcag cttcctgaag attatacgaa agaagatctt
    99121 gtatgtatct acgaaaatac cgctcaaatt cttggactaa actattacga gccacgtcgc
    99181 gtcaaatctc gttcttctat ggtggataca tctagtccat ttttaccaga gtggtttttc
    99241 gaaccacata tcatgcctgg acgacgaatg aacgaatacc ggggatggga aatttacgaa
    99301 aaaggaattt atgatttgtg catggatatc aagaaccatt atggaaacat cgaagcattt
    99361 atctcggaga acggaatggg cgttgcggat gaagaacgtt tcatggatga tacagggcaa
    99421 ataattgatg attatcggat tgagtatatc aaagatcatc ttgcttactt atggaaggca
    99481 ataaatgaag gatgtaacat taaggggtat catctttgga catttattga ttgctggtca
    99541 tggatcaacg cctataaaaa ccgatatggg ctggtttcac tcgaccttac tactcaaaaa
    99601 agaacaatta aaaaaagtgg cgaattttac aagcaattaa gtgatacaaa tggatttaga
    99661 tacgatgtag acttgttagt tgaataatat tctaaaattc cacttgaagt gaacacatta
    99721 gaaaacaaga ataatgagaa ataccaataa caaatacttt ttcttcatac ttcatttata
    99781 cttgtcagta taagagactg tgacaaatgt agtgatcctc agaagttagg tctggaaatc
    99841 taacttgaga agattttttt aaactacgtc cagtcttttt tatttgttta aaattgaagt
    99901 tgacaagtct tcattaaaaa tttagaatat tcacaagttt gacgacaatt atgaaaattt
    99961 atgtcgtgct aaaggagttt tgttattgga tgaaaagtta gatataaaaa ctgcgatcaa
   100021 agacacgtta cctaccgttt ttggttatat cggtattgga cttgcatttg gtatcgttgg
   100081 gaaagctgcc gggtttcatc cgctggtcgt cacgttgatg tccctgctga tctatgctgg
   100141 ttctgcccaa tttatcacgg tcagcatgct tgctagccat agtccactgc tctccatcgt
   100201 tctctcgacc tttctagtca attcccgaat gattctgatg agtatgacga ttgctcctta
   100261 tttcaagaaa aatcggctgc ttcaaaatct gctgattggc acattgctga cagatgaaag
   100321 tttcgcactg ggaatgaata aattgaacta tactggacaa aagttaaact ttcgttggat
   100381 gaatacagct aattggattt cttatttgac gtgggtgggt tcttctcttg ttggcgcact
   100441 tttagggaat tttattactg atccgaaaaa gtttggtttg gatttcgcca tcgtcgctat
   100501 gtttatcggg ttactttatc ttcaagtcat ttctgataga aacacctcta aacgattaca
   100561 gttgatcctg attggtctca ctttgattct ggtatatgtc ggactgattt ttatcccaag
   100621 taacttagtg attttagttg taacactgat tgggtgcgga ttgggggtgt ggatcaaaca
   100681 tgccttcttt tgagtttaca ttgctgacta ttttaggttg tactttagca acttggcttt
   100741 ctaggatcct accatttgtc ttactgaaaa aattcgattt gccgcaaccg ttgcttgagt
   100801 atttaagttt tgtcccgatc gtcattatgt ctgctttatg gttcagcagt ctatttaccc
   100861 aaaatatcgg acatctgccg cagatcaatc tcgaaaatgc tttggcttca gttcctacac
   100921 ttttagctgc tattctttca aaaagcttat tggtgatcgt cttggtagga attctttccc
   100981 tttcgttgat tcgcttatta taaataaaaa aacaagagct tatggcaaat tactgtcatg
   101041 cgctcttgtt tttatcaaag tcaaataaga aaaaaatcgg cgtattcttg cttatccgtt
   101101 gaacggacca ctaacaacga cttttcctaa tatatgtcct gtttctacgt ctttggttgc
   101161 ttgtttcaag ttcttcgcat tcatcccatc tgaatagacc ttgctcaatg tactgcggat
   101221 aatcccttca tttgctaaag cagcaatgtg cgctaaagct tctccttgcg tttcgatttc
   101281 atagttatag tctgttttag cgaacatata ctcccagtcg aagctgatag attggttttt
   101341 ccaaacacct atatctaacg gctctttgat ccctacgatc gttcccacat gtccaaatgg
   101401 tcggacaagg tgtttgattt gatcaaagta gcgggtaata tcgaataata cagcgacata
   101461 ctctacttga tcgattccta attcacgaaa acttttactt aagtcgtgat gataatcgat
   101521 tggatagtct acacctgttt ttttcagcca ttcaaaattc ttcggacttg cagtcgcgta
   101581 gacttctagg ccagcccaat gcgctagttg attcaaaatc gaaccgacgc ccccagctcc
   101641 gttgatcact aagatttttt tcccgcggtt ggcattttct tctggagtca gaccaaattt
   101701 ttcaaataac aattcataag cggtcaatga ggtcaacggc aaggctgcgg cttcttcatc
   101761 tgaaagattt tttggtgcaa gagcgacaat acgttcgtca accagttggt attcttgatt
   101821 gcttcctgct cttgtcgttg tacctgcgta aaaaacgcga tcgccgacag aaaatttttg
   101881 cacttcatca cctacagcaa caactttccc gactccatca aaccctaaga tacgtaaagc
   101941 atttcttatc ccgttatctt ggcgaagttt cgtatcgaca ggattaacag atactgcgtt
   102001 gacttttacc agaagatccc gtgaactggg tacaggtatc tgttcttttt catcaataaa
   102061 gctttttgga tcatcgatcg acaacccttc ataaaaaccg actaccttca ttaattcctt
   102121 ttctgacata cgaattcctc cttagcttca tcaagttgga gcatccatac agttttcatt
   102181 tcaatatata tagtatgcta tttgttccac ttttattcat cataattatt ctcttactta
   102241 aagtaaacta tttgcacttt tttggttaaa aaagaggttg cgacaagact tttgtcacaa
   102301 cctcttttaa acggtgttca atcaactgac cctcggtatt cgctcaaaaa acgacaaaat
   102361 atgaggagct atttttgttt ggctgttctt attacttgat gcaggacagc tttttcacga
   102421 cctctaattc acggtgttca agcaagaaat cattgttcac tgaaaagcgt tggatgttct
   102481 ttttcaaagg tgtctaagat ctctcgtact tcatcagtag atttcgtatt catcaactga
   102541 actcgcaagt catttgctcc tggaaatcct ttgatataga ttttaaagaa acgatgtagt
   102601 cctgtgatgg aacgcgggat gatttctgaa taatgatctt gcagatctag ttgcagacga
   102661 agtagtccga tcagttcttt ttctgtatgt tccctcggtt ctttttcaaa tgcataagga
   102721 ttcttgaaaa tcccccggcc aatcatgatg ccatctacgc catatttttc ggccaattct
   102781 tctcctgtct ggcgatctgg gatatctccg ttgatcgtga tcagtgtttg cggagcgatt
   102841 tggtcacgta aagcaatgat tttagggatg acttcccaat gtgcatctac tttactcatc
   102901 tctttacgcg tacgcaaatg gacagaaaga ttggctatat cttgatgaag taggtgactg
   102961 atccattctt ccatctcaga catttccgta tagccgatac gcgtcttgac gctgacaggc
   103021 aaaccgccag ctttagccgc ttcaatcaat tgtgccgcta cttctgggcg aaggatcaag
   103081 ccactgcctt ttccacgttc tgctacatta gggactgggc agcccatgtt gatatcgatt
   103141 ccttggaagc ccatttctgc catagcaatg ctcatttctc taaaaaattc tggcttgtct
   103201 ccccaaatat gggcaaccat cggctgttca tcttctgtaa aggtcaatct tcctcgtaca
   103261 ctgtcttttc catctggatg acagaaactg tctgagtttg taaactcagt aaagaagaca
   103321 tccggtgctg ctgcatgctt tacgacatgg cgaaatacaa catctgttac atcttccatt
   103381 ggtgctaaaa caaaaaaagg ctttggcagc tctttccaaa aattagtagt catttatttg
   103441 ttactctcca ttctatcaag cagtgaagtc gcaatctttc tcaaaaacat tgtcattata
   103501 ctaggaaaca aaaaaagaag caaagtgaac agcttaaaag atgtttctga ttgagcaaaa
   103561 aaagactgtg acaaaaatct tatcacaatt ccttttccgg aataaatggt gttcaaaagc
   103621 ttgcatctgc ggtaataagt ccaaccaaac gaaaaatagg aggagctatt tttgtttggc
   103681 tgttcttatt acttgatgca gaacagcttt ttcacaacct ctccactaaa cggtgttcaa
   103741 tcagctgacc ctcggtatta gctcaacaaa cgacaaaata tgaggaacta ttttcccgtt
   103801 tgtttctcta atactcaggt caaaacgctg atttcacaac ctcttttcta acttctcaat
   103861 cctcttcctt tattgatcaa ataccaagta acggcgtaca aaacaacgat gaatagcaca
   103921 agtacgatca tcgatgttgc tactggcaca tcgattgttc caaggaatcc gtaacggaaa
   103981 ccgaagatca tataaacgat cggattgact ttcgaaacag cttgccaaaa tggcggcaac
   104041 atagagatgg catagaaaac gccacctaga taagttaacg gctgcaagac aaatgtaggc
   104101 acgatcgata catcgtcata agactgagca aaaatcccgt tgagcagtcc tgctaacgaa
   104161 aacaagattg ccgtcatcaa taatgtaatg atgaccatga accatgaata cacatgaagt
   104221 ggtacaaaga acaaggaaat gatcgttacg agagagccga cgagaatact gcgacccact
   104281 ccgccaatca caaagcccca aatgatcaca tgcgtaggaa caggcgctac caagatttct
   104341 tcgatgtttt tctggaattt ttgagagaag aaggacgaag aaacgttggc atacgaacta
   104401 gtgatagcag acatcataat caaaccaggc acgatgaatt ccatatatga aaaaccaccc
   104461 atgtcaccga tccgaccacc gatcattttt ccgaaaataa caaagtacaa ggatgtcgtg
   104521 atcacaggcg gaaccagtgt ttggacccag atacgcaaat agcggttcgt ttccttgata
   104581 gctaaacttt ttaatgcagt aaaataaaga ttaaacattt ttctcctccg tatgattatt
   104641 ttcttctgtg attttcaaaa acagttcttc taaacgattc gacttattcc tcatagaaag
   104701 aactttgatt ccttgtcttg tcagctgatc gaagattccg ttcacccctt gatttcgttc
   104761 aacttcgaca gcgattgtct gttcatcttc aaatgtacaa gcgtatcctt caatcacagg
   104821 tttggtctca tatggcgcta aatcaaagat aaatgtttca aattgcagtt tggatagaag
   104881 ctctttcata ctagtattct cgatcagttc acctgattgg atgatcccga tgttgcgaca
   104941 aagcatttca gcttcttcta aataatgcgt cgtcaaaatg atcgttgtac cattttcgtt
   105001 caattctcgg agaaactccc acatctcgcg tctcaattca atatcgactc ctgctgttgg
   105061 ttcatccaag atcagcaaac gtggttcatg catcaatgcc ctagcaatca tcagtcttcg
   105121 cttcattccc ccggaaagca tacgtgcacg gacatttctc ttttcccaaa gattggactg
   105181 cttcaaatat ttttcgctgc gtttcatcgc ttctttgcga gagacaccgt agtagcctgc
   105241 ttgattcacg acgatttgct ggaccgtttc aaagggattg aaattgaact cttgcgggac
   105301 taatccaatc tgttgctttg ctcggactag atccgtatcc agatcataac cgaagacatt
   105361 cacttttcca gatgttttat tgactaaaga agtaatgatt ccgatcgttg tagacttccc
   105421 agctccattt ggtcctaata gcgcatagaa atctccttct ttcacggaaa gatcgattcc
   105481 tcgcaatgct tcgaccccac ttccataaac ctttttcaaa ttatttattt caagcgcatt
   105541 tgccatttat ttcctatctc cttatagaca tgattcttta ttgtatcatg gaatacagca
   105601 gatgaaaaaa caacttggtt ccagcgaaaa atcaggttgc tgggctcagg aaaatctcct
   105661 aatgaaagac atcaatcgga cgaagtcaga caaccttttc agaaaaaaca aatcgaagat
   105721 aaaggattac tattcatatg tgcaacaaaa ggagcgtgag acaaaagtaa agaacacttt
   105781 tgtctcacgc tctaaacacg tataaacggc ggaagcaaaa gccaagattt ttgatccaat
   105841 aaatactttc gatcacttcg cctatcccaa tacatgccac tgccgctttt catcaaataa
   105901 cgcggcttcc gtttttgcgg tttctactaa agtctctggg taatccaaaa ctttcagcaa
   105961 ggcaatcgca tttcgggtcc ttgaaggtcc ttcttttaat ttgaaatcaa aactgattcc
   106021 ttttccttct gttacttgtt cttcaaagtg gacattctca caataattct ttaaaatctc
   106081 tgtcaattcg atatcatgtg tggcgacaaa tgctaaagat ggatactccg caagccaccg
   106141 aacgactgaa gcggaagaag caatccgttc gatcgtattt gtccctttta aaatctcatc
   106201 tacgaagcag tagcagcgtt ctttggaagc cacttggtcc aacaaacgtt tgatcgattt
   106261 gatttctgaa acaaaataac tgtcgccttc aaataaatca tcttcgattg ccatggaagt
   106321 caacacatgt ccaggttgca gggtaaactg ttctgcaatc gctgtttgga tcgtctgtgc
   106381 tagaatacag ctgatggcta tacttttgac atacgtagat tttcctgatg cattggaacc
   106441 cgtgactaat gtattcttct gccagttgac cggattcact accgcttctt ttaacaatgg
   106501 atgatagata tctttcgctg tcacaccgcc ctcagtaaac tctggtcggc aagtgatggg
   106561 catataagta cggtaattca atattgcagc ggctacttct aattctccca gcaattccca
   106621 cagcttgatg gcttcagttg aatgcttttc taatttagtg agaacgaaat gataggagat
   106681 aaatggcagc atgaacatga tgttgacata ttcaaaaatc aactctgctt cactcccact
   106741 cttcatacga aatgaaaaac ctaatttagg gatgtctctt aatggtttag tttgctgttt
   106801 cagctcttct tgaagtggtg tcggcagttt tgctagctga ttggcggctg aaatcgttga
   106861 aaccaaataa ctcatgctat tcagctctgt ttctagtgtt tgtttcttca ctagataata
   106921 gaccgtattg aatactaacc caccgattgc caaatagatt cctagtactt gtcctaaaag
   106981 aagcaaaccc aaacccacca gcggaagcaa gccaagaaaa ataaataaac ctacgcgtcc
   107041 aatcttttta ctttttccat ccgctagata ttgttttgca aaattatgat ctttttttcc
   107101 taaacgagcg aattgatatt ggattttttc tctcagttga ggattttcct gataaaaagc
   107161 aatcagtttt tctaactgct gatcttcacc aaaatcaaag ctgcgtaaac gttgatacaa
   107221 cgcttctgaa cctacactag aataggtact gttgattcct tcgaatactt cgaacatatc
   107281 caggtcatac caagtaagat catcgatttc gctatcccat gaacggaact tcttctccgt
   107341 gagccatgct tccttcaagc tttcttcttt atcaaatctc ggttgataag gaattttttc
   107401 ccatttgcta cgaactgttt cctttaattt caaacgtgaa aaaatgtcta aagcaactaa
   107461 taaaataaca gctgcaataa taattaaaac gataaattct ctttgaccca tatccctctc
   107521 cgttccttta tttgttagct ttagtatagc actgttgcgc tatttgaaaa gagggtaaaa
   107581 aaacaccttt caaaataaaa ggtgtttttt tcttgtatat tacatattta tctctcattt
   107641 atcaaaattg acaaataatc atagggagga attgttgtta attcttgttt attctccagt
   107701 gcattgacaa ctgtcccaag ggaaaggata gcctcaggta ctattttttc ttgttctgtt
   107761 ggattagcta agcgaagaac aaatccttgt tcatgaaaat aagaaggata aatcgatgat
   107821 acaattaagt cttcaggtaa ttcaatcaat gaaaatttac gaggaacatc aagttcattt
   107881 tgtaatggcc aaatcttgtt atctaacctg tgaataaata cattcagttt ctgcttttga
   107941 taagaaatag attgttctaa ccgtttttct gcctgttttg ccactttgaa ctcattaaat
   108001 cttccttctt ctacattaaa tccaaaagtg actttatact cacctatctc ttgagctagc
   108061 ggtgtttcca tcatgacatg cccttcattt gtcgtatctc ctgaagctcg cccaggtcgc
   108121 caagcaagat taggttttcc aagttcccct gttgttgaaa ataaggttat gattaattga
   108181 ttttctatac gttcatactc ttttaaacca tctgcataaa acgtaaaata atgatcattt
   108241 tctgccacag agacactctt atcgaatatt tctaaattaa caggtttctc taccatagtt
   108301 tctgaccaat tcggaggaat gacatcaggg gtattttcaa taaatccgtt ttgaatttgt
   108361 gcaattgatt tttcagattt atctaaaaca ttaattgcta agcgcatacg atgcgagtaa
   108421 acagtattat gaattttgag tgtgccttca acgatttgac tttctcccag actgaacgat
   108481 atttcataag atatttcttc catttcgggt ttttcagata aacgatcttt taaatcttta
   108541 ggaagttgag cttttccgta aacaactaat gtttcttgta aagaatcttt atatacttta
   108601 gctgtttcta attttagaga tagttctgta tctccttcta atggtgaata atcatacgta
   108661 tcgccatcat ttgctgaatc gatcaaatga acgaaatcaa gatattgtct atctccaact
   108721 atcaaattca attgcccatc tttaaaagaa agtttatatt tattgttact tatctcagtt
   108781 ttgtctatct cgattgctgt ttctaatttt tcattatttt tctcaaattc aacaacagtg
   108841 tatcctagtg ctggcacatt tacatttatt tttatgtcaa gttcataata agctggttca
   108901 tcaaaatatt cgaaacctgt agcaatcatc cgataagcat ttttccgttc tggaaaataa
   108961 acttcttttt caatgatatt atagggacta cctttgaagt gtattttctt tgaatcagct
   109021 actacatgca cgattttcga tccactataa ttctcagtcg ttgtgttgaa aacaaggact
   109081 tcattcgatt ttagctttaa tgcatcagcc atccgtttta caataaggtt ttcgattccg
   109141 tcagctatct cattagcttc tttgattcga tggaaaatat cttccgcaac attatctgaa
   109201 acacagccac caatactatc atgagcctgg ttttgcaaaa tctttttcca taaccgacga
   109261 agtaggcctt tactgatatg gataccattt ctttctgcaa tcagcatgag cggctcaact
   109321 cgttttagaa ttttttgttc caatttaaaa ttttctattt tcatttttgt tcttactgag
   109381 ccaatagaac gatgaacacg agagtaaacc ggctgacgaa gttcaccttt atattcaggt
   109441 aattcttttt tgtttctgat aacttctaca taagaaggat aatctgaaat aatattgtgg
   109501 aatttactgt atttattcaa cttctctacg acttgatcaa aatttaaaat cattgacttt
   109561 tgatctacac ccgaaggtac taaaatatct ttgttttttc cttgcatatg aagcatttca
   109621 actgcttcat ccaacttttc ttttgcaaat tcaggaatat ctttaattgc gtctaaggtc
   109681 atcattccag tcatataacc aaatggaaaa ttcatggcat aaacaaattt atctcccaat
   109741 cctttccaga taaaataagg agatggaacc atcgtttcaa agtttaatcc tcgccaacac
   109801 atgaaattat cgatttctac ttgatgcaaa agtgtaggca aattcgcatt gaacccaaac
   109861 gtgtcaggca aataccctac catcattgga tctccatatt taaggatcgt atcactgatt
   109921 ccaatcatta agtttcttaa aattgattca gcatccacta ataaggcatc tgtttgtgca
   109981 taccacggtc ccacgaatag ttgtcctgtt ttcatcagtc gttggataac cttaagcttt
   110041 tgcggattga tctccaaata ctcatcgacg attgaagttt gtccatctaa acaaaagttt
   110101 actttagggt tcctttccaa ttcatctaga acttcagtaa agacctgatc agacaatacg
   110161 atggcatctt catttgtaaa ataccattct ctgtcccagt gcgtgtgttg aattatatgt
   110221 gcatttacca tatcagattc tcctactttc cttcttctac tgaatttact tgttgttctt
   110281 ttttatactt gatggatttt agtgcattga ccattaacgc tgtaaccact cccccaataa
   110341 caattgatac taaatataaa ggtatattcg taacaaaagg aattgccata aaggttgaaa
   110401 gcattgtttc ttgagtaaca ccaaaaaatc catttagacc acatgctact gcagtgccca
   110461 ccacgatgct tggtattact cgtaaaggat ctttcacggc aaacgggatc gtcccttcag
   110521 taatccccac aaggcccata atcatagctg tctttgcatt atctcgttcc gattcatcat
   110581 aaagtttttt gtttaaaatt gttgctaaaa acattccaaa tggcggaata gcaattccta
   110641 ataacatggc tgtactaggt ccaaagcttc cgcttgaaaa agaagcaacc ccaaaagcat
   110701 atgcgatttt attgataggt cctcccatat caaacgccat cattcctcct aaaatagccg
   110761 acatcaaagc agcatttgct cctgacatgc cttctagcca attagtcagt cctgttgtca
   110821 ttgcttgaat aggcgtagct agtacatacc ataatatgat tccaacaact aatgtagtaa
   110881 caaaaggaat cagcataatt gattttagac taataacttg catcggtact tttacttttt
   110941 tcaatgcatt agctaaatag ccacaagcta atccagcaac cattcctcca ataaatccca
   111001 taccaagatt ttgacacatc atcccagtga taaaagctgg tgctagaccg ggacggtcag
   111061 caattgaata tgcgatatag gctgaaatca tcggtacgat aaactgtaat cccgtctgac
   111121 caatcattcg caggactccc cagaatgtat ccgtctggtc ccaaacacct gtgccaccaa
   111181 aaatgactgc aatagaagtt agtaaccctg ctgctacgac aagcgggatc atataggaaa
   111241 ccccactttg gaaatgattt agaatttctc ttccttgttt cttgaattcc attagtaact
   111301 cccccacgat atatttttta tttgttaatt aatgcaattg cttcatcaat cgtctcttca
   111361 ccatctttta ctacctctga ggtccctact gttaaaatcg gtttaccgtc aaagcgacct
   111421 cgttctgaaa catttgtatc tactgcaaat atgacaacat ctgcctcgga aacatccgaa
   111481 attgaaagtt tgttttctat ccccatagcc ccctgtgttt ctactttaat atctaaccct
   111541 ttcttcttcg caaacttttc taaattagct tttgccatat aagtatgggc aatccctgcg
   111601 atacatgctg ttactgctac tatttttgtc atatgaaatt cctcctttta aaatgataga
   111661 gcttttacca attctttttt atttctcgaa tcttttattc ttttaacaaa ctcgtcatcc
   111721 atcaatcttc cagataattc tgctaaaata cgtaaatgat tagtaccttt atcagtgtct
   111781 tttattgcta acaaaaagac gagatcaact ggttgatcat ctaatgcgcc ccattcaaca
   111841 gaccgcttta acttggctac agccactgtt gattcaatta ctgctaggct tttgccatga
   111901 ggaatggcga tatttttccc aattcctgtg gtcatatggt tttctctgtc aaatacgtct
   111961 ttttgaaatt gtttgtggtc agacacaaac ccattagtct ctaacaattt cgataatttt
   112021 tcgactacac tttttttatc agatgcattt aaatctatca ctatattatt tatattgatt
   112081 ttttcactga tcatttataa gccttctttc aatatataaa ctaatttttc aaacgacgaa
   112141 ctcgttttta aatttgctaa taactcatga ttcgaagcga tttcattgaa tgtttcataa
   112201 attttattca attcaggttt tactttttca ttaagtgcaa aaaagaaaac agaatgaaca
   112261 agtgtcccat cccaatcaat tccttctgga ttaacgtaga cacagattac tggctttttt
   112321 ataaaatcta cttcgatatg cggcatagca aaatcattca tcgctgttga agatagtttt
   112381 tctcttttga ttgctccctc tagtatccct tctttcgcgt aattttcttt taccaatttt
   112441 ttggtaatta tatttaaaac gttcacttga ttatccttta tttggtgggt aaaaaagcaa
   112501 aatcgtttat ctaataaatt tagaaaagca tcttctgttt gttcatttaa ctgtagatac
   112561 tgttttactt ttctgacatc tgcccctaac atcaaaggac taacaacaat aacaggaaaa
   112621 tttgtatttt caatcggaat cgtgcttata acaagttcat cagtgacttt tctatcttgt
   112681 agttcattga tgcttagtac atcttctatc tcaaccatat ctgcaaaacc gttatttatt
   112741 ctttgctcta ataattttga tgttccatat ccgctactac aaactaatat cactttcgtt
   112801 ttgtcaggtt tgtaacgatc caaaagtgat tgaatatgga gtgttacgta agcgatttca
   112861 tcttcattaa aatgtataca gaatctctct tcgatttcct ctaataaatc tacacttatc
   112921 ataaatgacc ttttaaaaga atatttgatt ttgtccgtat aagggttata gatatttact
   112981 cccaatttga gtcttttaac tgctgcattg agatgtaata ctagattatt gatcaattct
   113041 tcatcaggat aaaattcatt taatttaatt cttactaact cttgtagctc aacacccata
   113101 tctaaatagg aaagcttcgt ttgatctaag tcgtgattca tagcattttt ttcaatcgaa
   113161 gtaatgtgaa tgtcaacata agcttgttca tctattggca caataattga aaatgctttt
   113221 tcaatcattt tgattaaaat aagtgtattc tttgagattt cttcttcaga attattacta
   113281 cgtgataaga aattgcttgt ttcaatcctt tctatcgcaa tagcaaggtg gatagctaaa
   113341 gtttgaaatt cataatctgt aaatttcaat tgtgtcttat tcatgtatcg attaagcaat
   113401 tcggctactt ttttcaatat gtccttattt aagaaattat tcgcttcacc aatgaaattg
   113461 atattcaagt ctaagagatc tgttttactg ttagatgctg taaagccacc ccaataatag
   113521 ttcacaactt ctgacatcaa ccttctttta cttttttcat cagctttgat gcgcacgcca
   113581 tttcgatctg aaactaagga catcccatgc ttgtttagtc tttttctaac atctttcatt
   113641 gtgctttcta ccgtcgacct gctcacgtat aattcgtcag caagattttg aatctttaga
   113701 gaatcattag tattcaataa tttagcaaac acataagtta gacggtcctc cggagagtct
   113761 gcgatattga ttttatgact ggataataaa tttcgaagtg tttctttttt tccaataagc
   113821 gaaacgcctt ttttaggttg aaccaacatt tgtacatctg gcattttttg gattatttct
   113881 ttcaaaatta aaatatcttt gctaattgtt ttcgtagata catataattg ttctgataat
   113941 tctttgtaag ttgtgtgctc attttcaatc aaaaagtata gtattttcat ttgccgttcg
   114001 ctaaattcga tgcccattat cttcacctcc tgaatacagc ttaagtgtac aattattatc
   114061 tctattttca tacagaagaa agttcttgat taatgtagaa gtttctaata ttcttgatta
   114121 tttcaaatca ataaaaaagc ctgcaatatt tatatcacag gcttttttct ctattaaata
   114181 tatataagat aatacatttt tctactagtt ctcactaatc ttgtataatt aaaccctccg
   114241 caacttgacg gagatggaga agatgccccc gtctaatcta gcggtcatct ctcctcccat
   114301 tccttccacc aatttttttg acaggtaaag cccgattccg ctagattccg tatttgtgcg
   114361 gctggtattt tcggtataaa aacgctggaa gactttttca acagaggttt gcgttcctgg
   114421 tttgatagga ttggccactg ttaatcgaat gaatggatcc tcttttgaaa atgtcacctt
   114481 tgcttgtcca tctgcataac gcaataaatt ctgtatgata ttttgcagca ctcgcagcaa
   114541 catgagcgga tctgctaata cagttgtatt tatcgagtgt tcagcgaacg ctacctcgat
   114601 atttttctca gaaaattgtt cataaaaaga gaaaaatact tcttccacct ctttagaaaa
   114661 agaaagcgta tggagatcca tttctttttg cttcgtctct attcgagcaa gatcataaaa
   114721 ctgatccgtc atactgagta atcttgcaac agattgtttg atgatcgtta aatacttctc
   114781 ccgtttctcc gtatcctcta cctgttccag taattgtaca taaccgttaa tagcagtcaa
   114841 aggagttctg agatcatgag aaaggccgac gatcgatagt ctgatgtttt cttccatttc
   114901 ttgaaatgct tgtccttttt gttcattttc atccaaccat gtattcagtg catctactag
   114961 gtccattaga ttcttttctc gaaattcgac tgaaagacga ccaccatatt cttgtcgttc
   115021 agaaagagtg gcgattttct tagttaatct cttgatttcc ctttgcagga agaaaataaa
   115081 acttcccata aagataacaa cgagaagcaa cagccattta atcaaatcta aaccccactc
   115141 cccacaccgt atgaatatac acagcatgtg cctttgctag cttccctcga agattggaaa
   115201 tatgcatatt cactgttttc tcactatcaa aatatggttc ttgccaaact gtttcataaa
   115261 gatttgctcg cgaaaatact tttttcggat gttctaaaaa aagcttcagc agatcgaatt
   115321 ctcttcctgt caaatggaca gccacaccat tcacatttac atccctcgac tctttgtcca
   115381 gtaagatttc cttgtaatag atgcgttgcg acagatcttt tacttggaca gaagtctgat
   115441 gacgaagttg gatgccgatt cgtaacaaaa gttcttcgat atcaaatggt ttcgtcacat
   115501 agtcatccgc tcctgctgcc aacaaggaaa ctttatctct ttgatcgctt tttgctgaaa
   115561 gaacgatgat cggaacattc gatgtctgtc ggatttctgc taacaatgct tcacctgaaa
   115621 gccctggaag catgagatct agaaggatca aatcgacagg atatgtgctt aacaataatt
   115681 ttccttccgt acctgaataa gcatcaagaa gtttgtatct ctgttcaaga acctcctcca
   115741 gtaaggaatg aacagcttca tcgtcttcga taatcaaaat agtttccagc aggaccacct
   115801 acttaatgtc agatttttgg aaaataaaca ttcctagagc tgatgatacc acgattgtta
   115861 ctagactgac cagaatcatt ttcggtgctt gcgaataagt tgcttgttgc gcaaagtccg
   115921 tatacatgag ccacgtttcc gtaaaccact ccgttagaaa ttccaagcct ttgatttttg
   115981 cgaaaacacc gggaatgaag gccgttccca tgatataaag aaaagaaaga agcaaactga
   116041 cactggtact tctagtgatg aatgtcaaag ccactggaac agataatgtc cctaatgtca
   116101 gcagaagcaa tacaggaaat acttttagaa cataatttcc atagtttttg attccctcaa
   116161 cttcaccaaa tccgaaagca agtgtgtaag caagcatggc tagtcccgtg atcaaaacca
   116221 cgccaagtgt tgtgatcaaa agcgtaacga gaagtttcga cacataataa gtcattcgat
   116281 tccgtcctaa agataacgta ttgcgaatcg taccgttttg aaattcttcc ccaatataaa
   116341 agcacgcaaa tgctgcgaaa gccagaagaa tccatgcagc cattttttct accattaaaa
   116401 tccctgttgc accgttgatg actgtatccg ttgcttggtt taattcattg ccgtattttg
   116461 tatgcatata ccattcgttc caagaataaa aaagcggaag tacgaccatc agtcctagtg
   116521 agacccacat tttacggctc ttccatactt ttattttttc agcagttaat agattaaaca
   116581 tgtggattgc ctccagttaa ttgtaagaaa taatcttcca gtttttgccc aactaggtga
   116641 ataccatcca caggaacttt tgcttctacc aatgcttgat tgatggctgc tacttggtca
   116701 attccttcaa aaagctggat aacacggcta gattgtacaa aataatccct ataaccaaga
   116761 gagtcgagaa ccgttactgc ttgttccgtc gccgaagtgt caatttttat aaattgacga
   116821 gattcttgtg ccaattcttc tttcgataat tctttgatca agtgtccttc atgcaaaata
   116881 ccatagtgag tggcgatctg tgacaattca tctagcaagt ggctagaaag caagacggtg
   116941 atgccccgtt cttttaccaa acgttggatg atttctcgca tttctgccat ccctgctgga
   117001 tccatcccat tcgtcggttc atccaaaatc aaaaattctg gattcgtgat cagtgcattt
   117061 gcaatagcta aacgctggcg catacctaaa gagaaattct ttgctttctt tttccctgta
   117121 ttttccaatc gcatcatttt cagcaggtct tcgatttctc tatccgacac accgccgttc
   117181 gctgcttgga tacgtaggtt atctcttgca gttaattctg gataaagagc aggggtttca
   117241 atcgattgac ccatacgtcg tcttgctgcc tgcaaatccg tcgttccaaa taattcgatc
   117301 gaaccttcat caatcgagat caaacccata atagttctca taaaggtaga cttacccgct
   117361 ccattttccc caatcaatcc atagatcatt ccacgtttga tttcgattga tactttatct
   117421 aatgctttgg cagcaccgta ttttttggtt ataccagttg ctttcaagac cgtttcattt
   117481 ccaaaatcca tacttatcct tctttcttat tgatgagtga agtatagttg acgggtataa
   117541 agaaagcagc cagaaatgta taaagaaagt gtaaattttc caattgaatg tcaaaaaaaa
   117601 caatcggaca atacgtgtct ttcctattat caggcagttt gttatacgct tgtatcagtt
   117661 tcctgattct ttcttttcga ccaaagtcgt ttctctgtat tcctcttcac tgaaaatttt
   117721 tgcttctttc agatcaagct tgagtaattt taccagaccg gttataagga taactatagc
   117781 aagactccct cccagaaaaa ttagactgtc tcgcagacgt ggttgcgttg tcttccaatc
   117841 aaagagcgca ccaaacagtg ctaatccaag tggactggcc accatggtaa tcgttgttat
   117901 cgttgacata agtcgtcctt gtatatgcgt tggaacttct cgctgaagcc agaccatata
   117961 aaaagcaaaa gaaaacatta atccttaaaa acggacttat gaaaaataat acatgccgtt
   118021 tgggatttac aattagctac caaataagag gttatgacaa aagacttttc acaatccctt
   118081 cttccgaata aacggtgttc tcctgccaac tcctcggtca caagtcacaa tatttatcaa
   118141 acatgagaag caatttttgt aaattccttc ttatttcttg aagctgacca cttctgtcac
   118201 aacctcagaa gcggataaat aatattgttt gatgatactt tatctttttc ccgtttgttt
   118261 atagatcgaa tgatattata gaccaagaat cctacctatt aacatagcta aaaacacaaa
   118321 aaaggcactt tccaaattgt ggaaagtgcc agaaaagtta ggattaaggt aactgcgctc
   118381 agcattgctt cgcccagtac gattctcgta actcgtgaac tcgaacgata atcgcaatta
   118441 acgttttgag aactgtgaag ctttacgggc tttcttaaga cctggttttt tcttcgtatc
   118501 attgaataag tttgaaaaac cttgatttat aaaggttttc ccgtaaatat attttattat
   118561 atttgattaa atttgaaatg tttgtgttct aaaatgtgtt ctaatttaaa ggcagtatat
   118621 ttcgtatttt agattaaatt tttaggctca ttcattctca aatccattct attcagtcaa
   118681 taaaaatttt aaaaagactt tataaacaaa gatgttcaag cgagcatctt atttttttgt
   118741 gcttaatttg aaaggagaac atttcatgta taaaaaacaa aaaaagaaat tatgtgtatt
   118801 aactattttg agtttggctg ttcaagcaat tgttggagca attgctccta cgattacttt
   118861 tgctgatgaa attacccatc cacaaacggt aaccgtagaa ctagatttag cacatcaata
   118921 tgctgttgaa ggaactttta gtgatggtcg tccaatgtca gaagtcactg ttccgcatta
   118981 tgcagtgtac aacggcttta aacaagatat tttctgtatt gaaccaggag tcccgattta
   119041 caatgagttc actcctggct atgagaaaaa cccgttacca gatatgtccg ataaagccaa
   119101 attagtttcc gttttatgga aaaaagtagg tacagatgca gatactcata ttgtggcaca
   119161 gaagatgatc tggcaagaag ttaatgggta tacccttcac tcaataaaac gatcagatgg
   119221 tagtgccgta aatatcgcag ccattgaggc aaaaataaat caagccattg acgattatca
   119281 gaagaaacca accttccata atagtacggc taaaacagta ttagggcaat cgactactgt
   119341 gacggatacg aataatctga atttgtcaga gttcgatgag gttgtggaaa atgccgcaaa
   119401 cattgattat cgtgtgaatg gtaatcagtt gataatcacc ccaaatgcca attcaaaaga
   119461 aagtggtgtg ctgacgctta agaaatctgc tggtaccgga acacctgtcg cctataaaaa
   119521 ggctggccaa cagactttaa tggccggggc gattgataaa cctaatacct acacaatcaa
   119581 aattgatgtg gaaacggagg gttctttaaa gatcaaaaaa gtcgataaag aatcgggtga
   119641 tgttgtacca ggaacggttt tccatttaga ctttggaaaa actttacctg caaaagacgt
   119701 gactactgac aaagaaggca ttgctacatt ggatgagatt cctcatggta caaaagtaac
   119761 cattaccgaa aaatcagtac cggcgcctta tacgattgat accaccccca tgactaccac
   119821 cattaaagca ggcgaaacca tttacgtgac ttcaaaaaac gcacgggaaa aagggcaaat
   119881 cattctggat aaaagcggag ttgaaacagg gagtgatctt tggaatgaca attattcctt
   119941 agctggtaat acgtttgcca ttcgcaaaga cagtccaacc ggtgaaatcg ttcaagaaat
   120001 gaccacggat gaaaacggtc atgcggaaac accaaaagag attgctaatg cgttggaatt
   120061 gggaacctat tatgtgaccg aaactaaagc tagtcatggg ttcgtgaata cgttcaaacc
   120121 tgtaaaagtc gaattgaagt atgccaatca aaccgtagcc cctgttacca gtaacgtaaa
   120181 agggcaaaat caagaagtca ctggcgaaac cactttgacg aaagaagaca aagatactgg
   120241 ggataaagca caaggtaagg cagtctttga gggtgctgaa tacaccttgt ttactgctaa
   120301 ggatggaaaa gctgttaaat ggagtgaggc ttttaaacca gagttagcga agggaacgaa
   120361 aggttctgat gaaacggtca ctttgacttt agacgaaaag aaccaagttg ccgttaaaca
   120421 tttagctatc aacgagtatt attgccaaga aacaaaagcg cccgaaggat atagccttga
   120481 tgaaacaaag tatcctgtat ccatcaaaaa ggtggatgat agcgaaaaaa atgccgtgat
   120541 tacccgaaat gttacggcaa aagaacaagt gattcgcttt ggctttgatt tctttaaatt
   120601 tgctggatca gctgcgggca ctaccgaaac tggcttcaat gatctgacct ttaaagtgtc
   120661 gccattggaa gggaccaatg aaatcacagg tgccgaagat gaagcgacca cagcttataa
   120721 cgagcaatta gggtttgatg gttatggcaa gtttgaaaat cttccttatg gggattattt
   120781 acttgaagaa gtagaagccc cagaaggttt tcaaaagatt acgccattag aaatccgttc
   120841 tacttttaag gaaaacaaag aagactttgt gaagagtgag tatgtcttta ccattaccga
   120901 acaagaccaa aaacaaccaa ttaaaacggt gaccgttcct tacgagaagc tgacgaacaa
   120961 agcattttct gttagtttga accgtttaat gctctatgat ttgcctgagg aagaagatag
   121021 tttaacttct cttgcgactt ggaaagacgg aaacaaagaa ttaacttctc tggattctac
   121081 cgagctagtt gataaattga gttacaatct acatgaaatc aaagaagact ggtatgtggt
   121141 agcgcaagcc attgatgtgg atgcgacaaa agctgcccaa gaaaaagatg aaaaagccaa
   121201 accagtggtg attgcagaaa caagcgcaac gttggcgaac aaagagaaaa caggaacttg
   121261 gaaaattcag cataaattaa ccgccgaaca agttttaaat aaaactattg tcttattcaa
   121321 ctatgtgtat gaaaataagg aagcttttga agcaggcgat aagccagtag cgaaggatgt
   121381 tagcttgaat aaccaagccc aaactgtcag ttgtacagtg gaacaccatg tttctatcca
   121441 aacaaaggcc cacctagaaa acggttctca gacctttact catggtgatg tggtggatat
   121501 gtttgatgat gtgtcgatta ctcatgatgt actggatggc tcaaaagaag ctttcgaaac
   121561 aattctgtat gctttattac cggatggtac aaacaaagaa atttggaaat ctggcaagat
   121621 tgattatgaa gtaaatgata aagaattcac caaaactgtg cttgcgaaaa aagtagatac
   121681 cggaaaatat ccagaaggaa ccaagtttac ttttgcggaa atcaattacg ataaagatgg
   121741 gaatatcaat ggaaaacaca atgaagattt gaaagaaaaa tctcaaacct taacaccaaa
   121801 agaagttcca accatactaa gtacgccgaa acaaccggaa acaccaactg ttccaagtgg
   121861 ctctcaagaa tctagtccca cagtgaagac cttccctcaa accggggaga aaaattccaa
   121921 tgttttactg ttcattggtt ttactttgat ttttgcgacg gcgggctatt atttctggaa
   121981 tcgccggaac taaggtgatg agatgataca agtaaatcct aagaaccaac gtgcgcgacc
   122041 gcctcctaaa aaagtagtcg cgctattttt gtatccaaaa ctaacaatga aaagaggaaa
   122101 agaagatgga attgaaattt gtcgtgccag atatggctga aacctttgga aagttaagtt
   122161 atgccggtga aggagaaatt ataacagaag gatatggacg gaatactacg gtgattggtc
   122221 gcagttatca tttgtattcc agtaagcaac gggccgatga tattgaggtg gtagtagctg
   122281 cggaagccgg tgaaaaggat tttgatcaag accaaccgct aaaagccgtg aatccccatt
   122341 tggttgccaa aggctatgag attgagaatc gtgggtttac tgattacgtg ttgtatgttg
   122401 atgatttagt gaaagcatag gaggaaaaga agatgagatt aacagaaggt attgtcgtag
   122461 attcaggttt aacatttggg aagttgcgtt tttccgcatt acgccgagaa gtacggaagc
   122521 aaaatgagga tggaacaatt ggtaatgagg taaaagaacg aacctataat ttaaaatcct
   122581 ctgcacaagg gcgaatgatt caagttagta tcccagctaa tgtaccctta cgcgagtttg
   122641 cttatgatgc agaggtagag ttggtcaatc caatcgtgga taccgttgcc aattatgttt
   122701 ttcgagaagg aactaccgtt aattggttca ttaaggcgga tgatttagtc ttgaagcggc
   122761 agccgaatca aggaaatcct acgaaccaaa atgaaagaaa gaaataggtg aagattatgg
   122821 aaattaatgt tgaacatatt ttagattgtc tcgatcaata tggaaaaggc gagctgactg
   122881 aggaacaact cgttaaagca ttaacctctg atgaaaaaat gtttctgatt atacatcaag
   122941 gtttacttgg agaaaatagc gacagtgaag aagactctga tgtactagac tggttaggag
   123001 aagaagagtc tttctttata gttgtcgaag taaatgaaaa cttatgtcgg caggcagaat
   123061 ccattttgga agaaattggt gtggagatgc cggatgctat tgaaggcttt ttaaatcaat
   123121 tggttgaaac gaaacaactc ccctcctcgt agcagtaaat gactagcata actttttgaa
   123181 attgcctaat gaaatcctat tgggcttttt cgattggagg tggaaaccat caaaatgtac
   123241 aaaggacatc gcatacgcgc aggagatcag cacttggttt atcactttgt tcttggctgg
   123301 ctactcgctc tgttcatcgg ttggatgagc gtcttttatt ttcaagagtt tagacaattt
   123361 gatatttcta agctatcact ttctactatt gaaatcgttt ggtctataaa agatttagta
   123421 tgtctgcttg gaagtctcgc tttttcagga gctatgatat tgctttatat tcactttttt
   123481 cttgatcatt ggcgaagctt gtggcatcga caaaagctag ctcggatgat tctagagaac
   123541 cattggtatg aagtgaaaca aactcaaagt gaaggctttt tcaaagattt gaatagtagc
   123601 cgaaccagag agactatcag ttacttcccc aaaatctgtt atcgaatgaa agatggatta
   123661 ctgtctattc gcgttcaaat ttcacttggg aaataccaag atcagctttt aaaattggaa
   123721 aagaagttag aaagcggact gtattgtgag ttagtagaaa aggaactcaa agactcctat
   123781 gtggagtata ctttactgta tgatatgatc gccaatcgaa ttgggataga cgaagtagtg
   123841 gcagaaaatg ggactctgcg attgatgaaa aatcaagtat gggcttatga ttctttaccc
   123901 catatgttaa tcgctggtgg cacgggtgga gggaagacct atttcctcct caccattatc
   123961 gaagcactat tgaagtcaga tgcagaattg tttatcctcg atcccaaaaa tgcagattta
   124021 gctgatttag gtacggtgat gcctcatgtt tattcacaaa aggaagaaat ttctgcttgt
   124081 gtggaagatt tctatgaacg tatgatggct cgtagcaagg caatgaaaga aatgtctaac
   124141 tacaaaacag gagagaatta tgcctatctt ggacttccac ccaacttttt aatctttgat
   124201 gaatacgtgg cttatatgga gatgttaacg acgaaagaaa gtgcggtgat tttgaataag
   124261 ctcaaacaaa tcgtgatgtt gggtcgtcaa tctggtttct ttctgatttt ggcttgccaa
   124321 aggccggatg cgaaatattt aggagacgga atccgcgatc aattcaactt tcgcgtggct
   124381 ttgggtcgta tgagtgaact cggatattcc atgatgtttg gggaggtcga aaaaaacttc
   124441 tttatgaagc acatcaaagg tcgaggttat gtggatacag gtggcagtgt gattagtgag
   124501 ttttatacgc cacttgtacc aaaagggtat gatttcttag attcaattag taaattagaa
   124561 aagatgaatg attgagtgat cttcaatact gactttatac ttcaagtaac gtataatact
   124621 tttatatagt aggaagaaag gggataatta atatggaaag taatgattct tatttagagt
   124681 atgcaaaagc aaataaggat aagtttattt cttatattat caaagggaaa tcccccgaag
   124741 ttggacgaga cgcagttttt atggcaggga gtcctggggc tggaaaatca gaggttgcca
   124801 ttggattagc tgagaattat gataaccatg tgattattga tgcggattac tttcgcaccc
   124861 agtttcctga ttataatgga aaaaactcta gtctctttca aaaagcatct tcttggttgg
   124921 tagagcaggc atttagatat gtcttagatc atggctattc ttttattctg gatgcaactt
   124981 ttgcaatatt aagtgctgaa aaaaatatct cgcgggcgtt gaaaaaacat tttcgagtga
   125041 cgattttcta tgtttatcaa gaccctaaaa tcgcttggaa atttacacaa gaacgtgaaa
   125101 ttgcagaggg acgtcatgtg ccgaaaaagg catttattaa cgcttttttc aaatcaagag
   125161 agaacattgc aaaagtaaag agtaaacatc cagatgtatt gcttcatatc ataataaaag
   125221 attatacgaa taatatttcc gaagttcatt atgctacgga taatctagaa ttatttttac
   125281 cactagggta tagtaggaaa gatttggagg ttcaattaca tgactgagaa agaacaagta
   125341 acgaaaattg taaagaaata caataagagc attgcggatt tatcggaaaa tgctactgca
   125401 aaagagttca aaacggtgat taaatatgta gccgatcagg ccaatgaaaa acaacggaaa
   125461 ttagttggat taaataaaaa atagtaggac aaatta
//
LOCUS       DACDIE010000003       114475 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.003, whole genome shotgun sequence.
ACCESSION   DACDIE010000003 DACDIE010000000
VERSION     DACDIE010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 114475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 114475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..114475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.003"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(300..2486)
                     /locus_tag="IV250_000244"
     CDS             complement(300..2486)
                     /locus_tag="IV250_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP8767560.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            2704..3387
                     /locus_tag="IV250_000245"
     CDS             2704..3387
                     /locus_tag="IV250_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAP8767561.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            complement(3468..4187)
                     /locus_tag="IV250_000246"
     CDS             complement(3468..4187)
                     /locus_tag="IV250_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP8767562.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(4499..4705)
                     /locus_tag="IV250_000247"
     CDS             complement(4499..4705)
                     /locus_tag="IV250_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8767563.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            4950..5552
                     /locus_tag="IV250_000248"
     CDS             4950..5552
                     /locus_tag="IV250_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HAP8767564.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            5693..6973
                     /locus_tag="IV250_000249"
     CDS             5693..6973
                     /locus_tag="IV250_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP8767565.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            6986..7714
                     /locus_tag="IV250_000250"
     CDS             6986..7714
                     /locus_tag="IV250_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8767566.1"
                     /translation="MIPIYICEYDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(7783..9306)
                     /locus_tag="IV250_000251"
     CDS             complement(7783..9306)
                     /locus_tag="IV250_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP8767567.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            9558..10739
                     /locus_tag="IV250_000252"
     CDS             9558..10739
                     /locus_tag="IV250_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP8767568.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(10799..12427)
                     /gene="treC"
                     /locus_tag="IV250_000253"
     CDS             complement(10799..12427)
                     /gene="treC"
                     /locus_tag="IV250_000253"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAP8767569.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(12878..15589)
                     /locus_tag="IV250_000254"
     CDS             complement(12878..15589)
                     /locus_tag="IV250_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP8767570.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGTIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(15599..16300)
                     /locus_tag="IV250_000255"
     CDS             complement(15599..16300)
                     /locus_tag="IV250_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8767571.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            16486..17085
                     /locus_tag="IV250_000256"
     CDS             16486..17085
                     /locus_tag="IV250_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP8767572.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            17166..18179
                     /locus_tag="IV250_000257"
     CDS             17166..18179
                     /locus_tag="IV250_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8767573.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            18287..19204
                     /gene="rbsK"
                     /locus_tag="IV250_000258"
     CDS             18287..19204
                     /gene="rbsK"
                     /locus_tag="IV250_000258"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAP8767574.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            19232..19627
                     /gene="rbsD"
                     /locus_tag="IV250_000259"
     CDS             19232..19627
                     /gene="rbsD"
                     /locus_tag="IV250_000259"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAP8767575.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            19627..21000
                     /locus_tag="IV250_000260"
     CDS             19627..21000
                     /locus_tag="IV250_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8767576.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            21357..23501
                     /locus_tag="IV250_000261"
     CDS             21357..23501
                     /locus_tag="IV250_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP8767577.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(23553..24593)
                     /locus_tag="IV250_000262"
     CDS             complement(23553..24593)
                     /locus_tag="IV250_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP8767578.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(24870..26672)
                     /locus_tag="IV250_000263"
     CDS             complement(24870..26672)
                     /locus_tag="IV250_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8767579.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(26686..27438)
                     /locus_tag="IV250_000264"
     CDS             complement(26686..27438)
                     /locus_tag="IV250_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8767580.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(27447..27809)
                     /locus_tag="IV250_000265"
     CDS             complement(27447..27809)
                     /locus_tag="IV250_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP8767581.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(27985..28857)
                     /locus_tag="IV250_000266"
     CDS             complement(27985..28857)
                     /locus_tag="IV250_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAP8767582.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(28908..29396)
                     /locus_tag="IV250_000267"
     CDS             complement(28908..29396)
                     /locus_tag="IV250_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAP8767583.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            complement(29534..30364)
                     /locus_tag="IV250_000268"
     CDS             complement(29534..30364)
                     /locus_tag="IV250_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HAP8767584.1"
                     /translation="MRLKHFLITGIGLMLTGGLLAGATYTLGAQKSLTWDNGPRLIDM
                     VTETKKIKDANKIVIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKK
                     STGAIISLEDTSAQITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSF
                     NEFTGGTITAKDELGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSL
                     HEMENSGYTIQTSGESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD
                     "
     gene            complement(30380..30988)
                     /locus_tag="IV250_000269"
     CDS             complement(30380..30988)
                     /locus_tag="IV250_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAP8767585.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(30981..31298)
                     /locus_tag="IV250_000270"
     CDS             complement(30981..31298)
                     /locus_tag="IV250_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP8767586.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(31454..32182)
                     /locus_tag="IV250_000271"
     CDS             complement(31454..32182)
                     /locus_tag="IV250_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAP8767587.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            32341..33222
                     /locus_tag="IV250_000272"
     CDS             32341..33222
                     /locus_tag="IV250_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8767588.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(33459..34502)
                     /locus_tag="IV250_000273"
     CDS             complement(33459..34502)
                     /locus_tag="IV250_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8767589.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(34505..35173)
                     /locus_tag="IV250_000274"
     CDS             complement(34505..35173)
                     /locus_tag="IV250_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8767590.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            35315..37651
                     /locus_tag="IV250_000275"
     CDS             35315..37651
                     /locus_tag="IV250_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8767591.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(37766..38077)
                     /locus_tag="IV250_000276"
     CDS             complement(37766..38077)
                     /locus_tag="IV250_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8767592.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            38258..39733
                     /locus_tag="IV250_000277"
     CDS             38258..39733
                     /locus_tag="IV250_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAP8767593.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPTMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            39780..40364
                     /locus_tag="IV250_000278"
     CDS             39780..40364
                     /locus_tag="IV250_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAP8767594.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            40366..40785
                     /locus_tag="IV250_000279"
     CDS             40366..40785
                     /locus_tag="IV250_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HAP8767595.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(41066..41350)
                     /locus_tag="IV250_000280"
     CDS             complement(41066..41350)
                     /locus_tag="IV250_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HAP8767596.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(41702..42412)
                     /locus_tag="IV250_000281"
     CDS             complement(41702..42412)
                     /locus_tag="IV250_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HAP8767597.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(42409..43881)
                     /locus_tag="IV250_000282"
     CDS             complement(42409..43881)
                     /locus_tag="IV250_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP8767598.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            44019..44492
                     /locus_tag="IV250_000283"
     CDS             44019..44492
                     /locus_tag="IV250_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP8767599.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(44645..45196)
                     /locus_tag="IV250_000284"
     CDS             complement(44645..45196)
                     /locus_tag="IV250_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP8767600.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            45301..45831
                     /locus_tag="IV250_000285"
     CDS             45301..45831
                     /locus_tag="IV250_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HAP8767601.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(46314..46757)
                     /locus_tag="IV250_000286"
     CDS             complement(46314..46757)
                     /locus_tag="IV250_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8767602.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(46773..47933)
                     /locus_tag="IV250_000287"
     CDS             complement(46773..47933)
                     /locus_tag="IV250_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8767603.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            48440..50140
                     /locus_tag="IV250_000288"
     CDS             48440..50140
                     /locus_tag="IV250_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8767604.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(50242..51075)
                     /locus_tag="IV250_000289"
     CDS             complement(50242..51075)
                     /locus_tag="IV250_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAP8767605.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(51379..53544)
                     /locus_tag="IV250_000290"
     CDS             complement(51379..53544)
                     /locus_tag="IV250_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8767606.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            53804..56098
                     /locus_tag="IV250_000291"
     CDS             53804..56098
                     /locus_tag="IV250_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP8767607.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            56091..56756
                     /gene="pgmB"
                     /locus_tag="IV250_000292"
     CDS             56091..56756
                     /gene="pgmB"
                     /locus_tag="IV250_000292"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP8767608.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            56749..57756
                     /locus_tag="IV250_000293"
     CDS             56749..57756
                     /locus_tag="IV250_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP8767609.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            57778..58797
                     /locus_tag="IV250_000294"
     CDS             57778..58797
                     /locus_tag="IV250_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8767610.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(59078..60445)
                     /gene="nhaC"
                     /locus_tag="IV250_000295"
     CDS             complement(59078..60445)
                     /gene="nhaC"
                     /locus_tag="IV250_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP8767611.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(60554..61975)
                     /locus_tag="IV250_000296"
     CDS             complement(60554..61975)
                     /locus_tag="IV250_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8767612.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(62218..64095)
                     /gene="tdc"
                     /locus_tag="IV250_000297"
     CDS             complement(62218..64095)
                     /gene="tdc"
                     /locus_tag="IV250_000297"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP8767613.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(64386..65642)
                     /locus_tag="IV250_000298"
     CDS             complement(64386..65642)
                     /locus_tag="IV250_000298"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAP8767614.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            66222..67523
                     /locus_tag="IV250_000299"
     CDS             66222..67523
                     /locus_tag="IV250_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP8767615.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(67592..68191)
                     /locus_tag="IV250_000300"
     CDS             complement(67592..68191)
                     /locus_tag="IV250_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8767616.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            68445..69326
                     /locus_tag="IV250_000301"
     CDS             68445..69326
                     /locus_tag="IV250_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8767617.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            69339..70949
                     /locus_tag="IV250_000302"
     CDS             69339..70949
                     /locus_tag="IV250_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HAP8767618.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(71026..71685)
                     /locus_tag="IV250_000303"
     CDS             complement(71026..71685)
                     /locus_tag="IV250_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP8767619.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(71968..72633)
                     /locus_tag="IV250_000304"
     CDS             complement(71968..72633)
                     /locus_tag="IV250_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8767620.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(72780..73385)
                     /gene="dhaL"
                     /locus_tag="IV250_000305"
     CDS             complement(72780..73385)
                     /gene="dhaL"
                     /locus_tag="IV250_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HAP8767621.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(73413..74396)
                     /gene="dhaK"
                     /locus_tag="IV250_000306"
     CDS             complement(73413..74396)
                     /gene="dhaK"
                     /locus_tag="IV250_000306"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAP8767622.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            complement(74398..74793)
                     /gene="dhaM"
                     /locus_tag="IV250_000307"
     CDS             complement(74398..74793)
                     /gene="dhaM"
                     /locus_tag="IV250_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HAP8767623.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            74947..75267
                     /locus_tag="IV250_000308"
     CDS             74947..75267
                     /locus_tag="IV250_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAP8767624.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(75379..76248)
                     /locus_tag="IV250_000309"
     CDS             complement(75379..76248)
                     /locus_tag="IV250_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8767625.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(76344..77648)
                     /locus_tag="IV250_000310"
     CDS             complement(76344..77648)
                     /locus_tag="IV250_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP8767626.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(77645..78154)
                     /locus_tag="IV250_000311"
     CDS             complement(77645..78154)
                     /locus_tag="IV250_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8767627.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(78329..80464)
                     /locus_tag="IV250_000312"
     CDS             complement(78329..80464)
                     /locus_tag="IV250_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8767628.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(80547..82202)
                     /locus_tag="IV250_000313"
     CDS             complement(80547..82202)
                     /locus_tag="IV250_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP8767629.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(82204..82518)
                     /locus_tag="IV250_000314"
     CDS             complement(82204..82518)
                     /locus_tag="IV250_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP8767630.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            82688..82948
                     /locus_tag="IV250_000315"
     CDS             82688..82948
                     /locus_tag="IV250_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAP8767631.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            82962..83276
                     /locus_tag="IV250_000316"
     CDS             82962..83276
                     /locus_tag="IV250_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAP8767632.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(84006..84560)
                     /locus_tag="IV250_000317"
     CDS             complement(84006..84560)
                     /locus_tag="IV250_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAP8767633.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(84686..86615)
                     /locus_tag="IV250_000318"
                     /pseudo
     CDS             complement(84686..86615)
                     /locus_tag="IV250_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(86921..87637)
                     /locus_tag="IV250_000319"
     CDS             complement(86921..87637)
                     /locus_tag="IV250_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP8767634.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(87638..89461)
                     /gene="glpO"
                     /locus_tag="IV250_000320"
     CDS             complement(87638..89461)
                     /gene="glpO"
                     /locus_tag="IV250_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HAP8767635.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(89475..90971)
                     /gene="glpK"
                     /locus_tag="IV250_000321"
     CDS             complement(89475..90971)
                     /gene="glpK"
                     /locus_tag="IV250_000321"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAP8767636.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(91107..92567)
                     /locus_tag="IV250_000322"
     CDS             complement(91107..92567)
                     /locus_tag="IV250_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8767637.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(92557..93852)
                     /locus_tag="IV250_000323"
     CDS             complement(92557..93852)
                     /locus_tag="IV250_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP8767638.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(94184..94861)
                     /locus_tag="IV250_000324"
     CDS             complement(94184..94861)
                     /locus_tag="IV250_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAP8767639.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(94891..95982)
                     /locus_tag="IV250_000325"
     CDS             complement(94891..95982)
                     /locus_tag="IV250_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAP8767640.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(96083..96517)
                     /locus_tag="IV250_000326"
     CDS             complement(96083..96517)
                     /locus_tag="IV250_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8767641.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(97101..98558)
                     /gene="ascB"
                     /locus_tag="IV250_000327"
     CDS             complement(97101..98558)
                     /gene="ascB"
                     /locus_tag="IV250_000327"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP8767642.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(98555..100450)
                     /locus_tag="IV250_000328"
     CDS             complement(98555..100450)
                     /locus_tag="IV250_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8767643.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(100508..102283)
                     /locus_tag="IV250_000329"
     CDS             complement(100508..102283)
                     /locus_tag="IV250_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8767644.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(102333..103829)
                     /locus_tag="IV250_000330"
     CDS             complement(102333..103829)
                     /locus_tag="IV250_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8767645.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(104023..105645)
                     /locus_tag="IV250_000331"
     CDS             complement(104023..105645)
                     /locus_tag="IV250_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP8767646.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(105973..107286)
                     /locus_tag="IV250_000332"
     CDS             complement(105973..107286)
                     /locus_tag="IV250_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP8767647.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(107531..107755)
                     /locus_tag="IV250_000333"
     CDS             complement(107531..107755)
                     /locus_tag="IV250_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8767648.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(107775..108143)
                     /locus_tag="IV250_000334"
     CDS             complement(107775..108143)
                     /locus_tag="IV250_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP8767649.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPGEITDNNGILT
                     FNFSDPEGNYFVIGKAESSD"
     gene            complement(108339..109049)
                     /locus_tag="IV250_000335"
     CDS             complement(108339..109049)
                     /locus_tag="IV250_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8767650.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(109067..109564)
                     /locus_tag="IV250_000336"
     CDS             complement(109067..109564)
                     /locus_tag="IV250_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8767651.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(109601..110053)
                     /locus_tag="IV250_000337"
     CDS             complement(109601..110053)
                     /locus_tag="IV250_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8767652.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(110188..110793)
                     /locus_tag="IV250_000338"
     CDS             complement(110188..110793)
                     /locus_tag="IV250_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP8767653.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(111243..111863)
                     /locus_tag="IV250_000339"
     CDS             complement(111243..111863)
                     /locus_tag="IV250_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8767654.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(111865..112734)
                     /locus_tag="IV250_000340"
     CDS             complement(111865..112734)
                     /locus_tag="IV250_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAP8767655.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(112757..113164)
                     /locus_tag="IV250_000341"
     CDS             complement(112757..113164)
                     /locus_tag="IV250_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP8767656.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(113183..114472)
                     /locus_tag="IV250_000342"
     CDS             complement(113183..114472)
                     /locus_tag="IV250_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8767657.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
BASE COUNT        33478 a        23678 c        20038 g        37281 t
ORIGIN      
        1 ttaaaatacc aattattagc taataaaata taaaaaaaat caaaataact gattatttac
       61 gtttgaataa tgagaatttt tctatatatt ctctattttg ataatagtat tctcattttc
      121 aaaataaata agggaggtgt gacaaaattc ttgtcacacc tcccttttcc gaataaactg
      181 tgttccagaa atagcttctt cgaaaataag ctgaaatttc acaaaaattt gagaagcaat
      241 tttcgtacat tccttcttct ttcttgaaac tgaccacttc tgtcacaccc tctcttctat
      301 cactttcctt ttttcacacg actcccggtt tctttgataa atctcgatac tttcaatttt
      361 cttgaatttt tcatttgagg tacacataga tacagacgat ttcttgctct agtcatcgca
      421 acataaaata gtcttcgttc ttcttctatc gtcgttactg gttctgcttt tatttcatta
      481 aagtcgatta ctttcttttc tgtcgctccg ttataaggca aacttccttc gttacaacca
      541 atcaaaaaga catttcggta actaagacct ttacttttgt gaatgctctg gatacatagt
      601 gcatcaagtt ttccttgtct gtaagcaatc aagcgggttt ttaattcttt agctgtcgct
      661 tgggtttctt gcgataaggc ttggagtctt ctgaaagtag catgctgctt tacttcttct
      721 cgtatccatt cttgatcact ctggtcgatt tccttagcta tacgtggcag atgaagatag
      781 ctttgaacaa gcgaatagat ttcttgatgc tggtctgttt tacgcaacgc ttcctcaaac
      841 tctcggattt ctgcagattt cgattttaaa ttggggctta tcgacaaaat ataatctgca
      901 acggctatat tttgatattt aggagaaaag tattgatcgc attcactttt tttcaggtag
      961 cagtctgcca tcagctgccg gagcaattgc tggtaggcct gatttttctt ttgcagattc
     1021 agtgctgttt gccaatgaac aattttttga tagaaatgtt ggaaagactt gataccaaag
     1081 ataccttcat atggactatc tgcttccagt acaaaaggaa tattcgtttt cagcagagat
     1141 tggacaagct gctgcgtttg tgaagctgat cggtataata tgattgattc cttatatttc
     1201 ccattttttt gaataaatgt ctgaatttgt ttgattagcc actgactttc tgctttagta
     1261 tcagaaaaca aataggcttc cataacacct gcttcttttt gtgctggaat catcgccttt
     1321 ggtatccgat catttttatt atagctgatc aatctatttg ctccttggac aatagctgcc
     1381 cgagaacgat aattagttaa taagaaaaga gttttggtag gatattcttt ttcaaagttc
     1441 ttgatgtatg ctggctctga tcctctgaat ccatagatac tctgatcatc atctccaaca
     1501 acaatcaact tgatcctgtt ttcctttgtc atccgtttca cgatttccca ttgaagtgga
     1561 ttagtatctt gaaattcatc aatgaataga taatcatatt tttggccgat ataatgctgg
     1621 atctcctcat tctccaaagc gcttttcaga ttttggagta catcattgaa ctctagcaat
     1681 tgctggcttc ttttttgttc ttggagttcc gtgagcaatc catacagtcg ttctgatatc
     1741 ggctgtcgtg cattcttatc aatggaatca tatttttctt taactaactg aaccatttct
     1801 tctactgaaa ggttcatatt ctgccaatag tctaaccgag aagcaatcgt tttttctcca
     1861 tattgatcaa atatataaag attatattta cgtatcagct gttgcaaaac agcattcact
     1921 ttttgtgcag tatcctttga aaagtctcct tgtattcctt gcattgctac ttcaggaaat
     1981 tttcgacaaa tttcattgta tatcctaaaa aagatactat ggaatgttcc gatatggaca
     2041 gcggtttttc tctggctgaa attatctact cgttttttta gttcatttgc agcgttcctt
     2101 gtaaaagtga tcattagtat gcgttcttcg tctacttgtt tttgcgtcat cagataaaca
     2161 gcttttgcac agattgtttg cgttttgccg ctcccagctc ctgctatgat tcgaagatgt
     2221 ttcttcatat catatgtgat agctgctttt tgttcttgat tgaagcgatt ataggaacgt
     2281 ttctccatca ttttaaagaa agagctgttt ccttcacttt cttttacttg tacttcttcc
     2341 tcaataagtc cgggtgcttc gccataagcc gcttcatagg tagcaatgat ttcttcttgt
     2401 agccgaaaga cttgttgaag ctttttatac ttgttctccc agcgtttcaa ttctttttgt
     2461 aatcggagaa tttctttttc attcaactga ttgatttcct tcccatcttt cttctttgaa
     2521 tttgtttatt tcaagttggt tatttaaagt ttcgttgtct attttttctt tatactaaca
     2581 gacctttcta aaaatgaaaa tgggaagcga tatgatttta gttttctgaa aatttttcct
     2641 ttttttcaat cgtttatgct aagataagga agacaaaaat attcataaaa ggtgatgaaa
     2701 aaaatgcaga cttcctttca cgaggggcgt acaataggac ttttgctgac atttatcgga
     2761 ggagccatgg acagttatac ttatatccaa tatggtgctt ttgcctctgc tcaaactggc
     2821 aatatcattc tatcgatcat ccaagcattt gacggagaat ggctaagtgt tggtaaaaaa
     2881 gtattgtcta cccttttctt tttcttaggg attcttctaa caaagtatct aattgattat
     2941 tttagaaaaa aagaaaaaca tttctggcgc ttgtttgttc tatattatga agcagtgatt
     3001 tttttctttg ttagtttgac acccgtccat gagcatcccg cattagtaac gattttgatt
     3061 gcttttactg catcgatcca gtgggtagct tttgataaaa tcaacgggcg tgcctacact
     3121 aatttattta caactggaaa tttaaaaggg gtagcaacga acttatatga ttattgtatt
     3181 tctaaagaaa aagcagattt tgatcgattt ttccactatc taagtgtcgt tcttgccttt
     3241 atcgcaggag ctattgtcct tgttttctgt catcatctgt ttggcgccca ttcgattttg
     3301 atcgtttcca ttttattttt gatcatggcc ctcactcaga catttcattt atggcaattt
     3361 tatcatacga atcggttcac tatttgatct caatgaatca aaaaaactaa gcaagatgct
     3421 gctcacagag aaatatctgc gagtgagcaa tttgcttagt ttttatatta tttagttgta
     3481 aagattccct ttgctttcgg gttatttgta gtgtagcgga tcgactgggc atctgcctct
     3541 tctgtcattt tctttgcccg catcttgatt tcttgtacga tttgttctcc acatgccgga
     3601 tcggctacga aatcatattt gtccccatcg atcactattt tgggacatat atcaaactgt
     3661 tcataccact cttcgtatcg tcgattgagt tctttatagt aggaatagag ttcaggatct
     3721 tgttctagct gttcatattc ccggcctctt tttttgattc tcgctaacat agtttcaaag
     3781 gatacacgaa tataaaccag tagatctggt ttcttttgtg gcgaaatttc ttctaactcc
     3841 ttcagcatcg tttctaaaag accttcatac tgctttactt ctacttcaga aacacgccct
     3901 aaatctgcat tcaaatgaaa cagcatacta tcttcgtaga tggagcggtc tagtacgttg
     3961 tcatcatggc tgaaagcatc tttgattgcc aaaaagcgtt tgttcaaaaa gaaaatctgt
     4021 aataaaaaag tatacttttc tggattagaa taaaatagcg gcaatacttc attatcttcc
     4081 acgttctcat aaaaaggttt tgtttctaaa tcttgggcaa gcatttcagt tagtgtgctt
     4141 ttccctgcac cgatcgttcc tgcaacaatg atcacaatta tcggcatcct tcctgtaata
     4201 aaatctgttc ttcattttac tacttcattt atagaaataa aagccttgac tttatctgct
     4261 ccttaaaagc tacttctcta aaagtagcca aaagtaactc ctattcatga aaataaaggc
     4321 tgtgacaaaa gtcttgtcgc agcccttttt tcaaataaac agtattctcc tgccaacacc
     4381 tcggtcacaa ggcaatattc ataaaacatg aaaagctgtt ttttaacatt attccttgta
     4441 actcagagct aaacgggaga ctcacaacct ctttatgctc tcaacatttt atctgttttt
     4501 attcttctat tgtacttgat ttgcgtacaa ctaactgcca gtgcagatta tgagcggtat
     4561 cattgtcttc tactgcgcat ttcatgacac gtatcatcac actattttca tagatttttt
     4621 ctatttttcc ttttattggt gattcaaata aatcagattt aaatgcatat gtttcgccga
     4681 cttcaaacaa ttgttcattt tccataagaa tccctccatc accttttata aggtcagtaa
     4741 ttttttaaca taaatcactc gaaaaagcac aaacttgtca taaaaagtac ttttcgtatc
     4801 aaaactgtca ctttttttgg taaggttctt ggaaaacgaa gattttttta ggagatcatg
     4861 acggttttga tgtcaaaacc actttttatg aaacaaaaga cgttttttat tttttttagc
     4921 ataaaatttg tggtggaggt acgtatgaga tgaaagataa agttgtgaac aaattattag
     4981 aagccttatt cagcggagac aaatcggaag aagatatggt gtatgtccaa gtaaaatttg
     5041 tcttagaagt tttattgaat aatcttggaa aactagcggt cgttctagtc ttttctctgg
     5101 tgacaggctc atgggcggaa acaggaatca cctttctttc ttatatctgc atccgcagat
     5161 atgcttatgg attgcattcc gactctgaat tcgtttgtct gctgtggaca ctactttatt
     5221 tatggggcgt tcccttggtg atgaaacatc tgcaattgac tatctccttc ccaatgatgg
     5281 tctttttgtt aatcagctgc tttttgttac tgcttcgcta tggatcaaga ggtacagcaa
     5341 tcaatcccat tgaacccgag aaacgtcccc cattattaaa gaaggcgatc agcatgtttc
     5401 taattttttc tctcattacg ttatttttct cagcttctta cttttctacc tatttgctac
     5461 ttgggattgt tttagaaata gctacgttgc ttcctattac aaactacttg atgaattttg
     5521 gaggaatctt tcatgaaaaa aacaatcatt aaacaattaa ctaacttatc tgactttttt
     5581 gtatctggct gctgctttgg tatgagttct gaattcaaaa tccctgaaaa attaaaaaaa
     5641 taatacgcca attgtgctat aatttggtaa aaatcaatgg gagctgtact ttatgttttt
     5701 atggtccata gcgttgcaat atttgattta cacaatttgt atcttattga ttgaccatca
     5761 catccgatac aaatatctgt ttgcgggagt cattctgatt tcttgtattt tgattggatg
     5821 ggtctattcg attttaggga tttttactgc tctaattgct ataggaatgc tgatgcttac
     5881 ttttttcttc ttttcgactt cgcgtcaaca attggtttac gcgcttcctt ttgggttatt
     5941 gacgtcgctt ttaggagatc acttaacaag catcggcgat ttttttgttt tgaaatcttc
     6001 ggttattgaa ccaggagatt cattgcagta ttttcacatt gtcttatccg cttttttatc
     6061 tgctttatta gctttttgtt taaaaaaatc gttagagact tggtttcaca ctgtggaccg
     6121 ccgtatgatc ggtacgatcg ggacattgat attatttaca tactatatca ttatttttta
     6181 tacccgtttt tctggtgaaa cacctgatgt cttagctttg aatacgagtt ttttcattct
     6241 atatctgtgt gttggcctga tcatcttggt ttattattgg aaaatctcta ataagaaatt
     6301 agctgatcag ttattagagc aacaagtcag gatgcaacag gattatatca aagatctaga
     6361 aaaaaactat caagacttga gagaattcaa acatgattac caaaatctgc ttttttctct
     6421 tcacagctat atttcagaag gcgacttaga aggattacgg tcttattatg atcaaacgat
     6481 attacctaca cgccagatga tgaacctttt tccggccaat ctaagtatgt tggataatat
     6541 gaaagttcct gagatccgta gtttactgtc attaaaactc atgatggcac aggaaaaagg
     6601 gttgatcgtc cagcttcatt ttcctgagcc aatcgaactg gatacgaaac atacggtaaa
     6661 ccttgtacgt atgcttggta ttatcttgga caatgcgatt gaaggagcta gtgctgcaaa
     6721 gaagaaaaaa atcgaattga ccatactcaa gaagaatcat tccgtgatga ttcgtgtgat
     6781 caatacaaca acaaatacat taccgttaaa ccagttgaaa caaaaagggt tctcaacaaa
     6841 aacaaatcca aaaaacgaag gccttggctt gtttatcttg gatgaattga caaagaccaa
     6901 tccttatctt ttccttgaaa catcaattga aaaccagcgt ttttctcaaa cgatttatat
     6961 acaaataaaa taaaggagta atgatatgat tcccatttat atttgtgaat acgatccaaa
     7021 acaattagaa gttttatcaa cggtgatcaa aaatagaatc atgattgaaa acttagacag
     7081 ttatgtccat atttctacct ctgatccgga agaacttctc aatgcagcca agatcagaac
     7141 ttcttctttt ggtatttact ttttagatat cgaactagaa gacagtgaaa tggatggtgt
     7201 agcgatcggt aaattgatcc gtgaattaga tcctttagga aaaataatct atgtgacttc
     7261 ccatacggaa atgtcgatga aaatcttaaa aagcaacatc gaaccgactg attatatcgt
     7321 caaagaagat ctttatgatt taaaagaaaa tgtcgagcat atcctgagca aaattttcga
     7381 agattttcaa atgtcccaaa tccaaaaaga tatcttcaaa atagagttta atgatgagat
     7441 caaattttta ccgatcaaag acatccaata tttttctact tcgctaggag cgccacataa
     7501 actggaagtc catttgactc atgcccaatt acaattctat gagaagatca aagaaatcga
     7561 gaaaatgcat cggaatttta tccgttgtca taaatcctat gttatcaata cacaaaatgt
     7621 gcggtcgatt aataaaaaaa ccagagaagt cacgttggca aacggcgaga ccatcccagc
     7681 ctctatccgt ggattaaaga aattgatttc ataaaaagaa ccgaatagtt tggaccgact
     7741 aaatgaccag ccgatccaaa cccttcggtt cttttttgcc tatcagtgca aaagcatttc
     7801 ctgtgcaatt tcacgtccat ccattgtatc tggataaaat gttggccaat tctccatttc
     7861 tttcagcaat acttcttgac tttcatttcc tgcatataaa tgaaaatgtt cggagtcagc
     7921 cggtgcaatc gtgtgatcac tgaattgaat atatctaaaa gcgccacttt ccttatcttc
     7981 tgcttcgaat agatacctta cgccacggtt tcctgcctcg taggtcaaga tttctttccc
     8041 tacataacga taatttgaac gtttcgttgt tccattctta aacgtaaatg ccatctcttt
     8101 ctcactgatc ttgattgaag caatatctgt ttgatagccc ttcgtataat attctttata
     8161 ttcttgtgct gtcttatcct gattcaattt ttctttatat tcaaaaacag gatcaaaagt
     8221 cccatcttct acatacgtat ctactgtttg ccaagttcct gcccaatctg aaagagagcg
     8281 atcttgtaca tcctcgtctt caaaataacc gttgtagacg ctcttttctt ttttgggggt
     8341 caaagtatct tctagcgggt tttctgtctc tgtcgttttt ttcaacgctt cgaggtttgc
     8401 ttccatgata gaaagatagt tttctccatt ttctatttgt tcattcgtca atccttccaa
     8461 aggattcaat acttctaaag atacacctgc ttcgtttgct aacgttctgg caatactatc
     8521 tttggcgttt tcttcaaaat aaatatagtt gattccatgt tctttaacaa agtgtttcag
     8581 ttcagcaatt ctcgccgcag acggttcttc actagaagaa agaccagcaa tcgatacttg
     8641 attcaatcca tattctaacg ctagatagtt gaatgcagca tgttgcgtga caaaagatct
     8701 ttgttttgct tgcttaaagg tttcttgata aagctgatgg agtgcttgaa gtttctcggt
     8761 ataggctgct gcattttttg tccatacttc ctgtttttct gggtaagctt caatcagctg
     8821 atcacgtatg tttgctacct gcttgattgc taaacttgga gctagccata catgtgggtc
     8881 tagctcatgt gtatggcctt cttccccgtg ttcatggtca tgatcatgtc cttcttcact
     8941 tcccggaagt aaaagcatac cttctgtcgc ttttatcgta tggacattgc cttcttgtaa
     9001 tgttttttgg attgcgggga cccatgtttc catgttttca tcattataaa caaagatatc
     9061 tgtatcttgg atctttgcca tgtctttagc agaaggttca taatcgtggg aatcagtccc
     9121 tgctggaatc aataactcta cttctccttc atccccgacg attgcttttg taaaatcata
     9181 catgggataa aaggtcgtca tcacgctcaa tttttcttta ctgtcctcgg ctttcccact
     9241 tgccccgcaa gcagttaaca tacctgctaa aaccagcatg gccaatatac ctatcaatcg
     9301 tttcatttat gacactcctc ttctcatctt ctttcatttg aatgttccga ttcgcttttc
     9361 gcaaaccgta atagttacgt tttagcaaca ggatttaatt tatcatatgg ttaaaagaac
     9421 tgtcaagcgt tttcttattt taattgatga gaaagtttat atgaatgatt gacgatattt
     9481 ttgcaatcaa cctctatcat tttcctttta tacccgcttt tgtctataat gaatttatag
     9541 ataaaggagg aaaaagagtg aaaaaagaaa cagatacaac gaatcgtttg atccgctttt
     9601 taggaggaaa aacctcttac tatgttttag gaatagttat cttacttgcg ctgatcattt
     9661 attttttcca tcagatctcg tttatattca agcctgtcgg aatcattatc gctactatct
     9721 tgcccccatt attgtttgca ctgatcttgt attatctttt ggaaccagtc atcaaacgtt
     9781 tgagcaagca cctttcgagg aacttgtcag tcattatcgt ttatgtgatt attttatttt
     9841 tattggggct tggaagcata tggcttattc cttttcttga agaacaggca aagacattga
     9901 tcagtagttt gccagatttg tttcaagatt ttcaagagag tctccgacaa tttttggaga
     9961 aaacaccttt tgccgattca tttaatcaat ttttcgcttc tattgacgac gtgacgtctg
    10021 atatagctgg atttattgga aattattggg aaagtggcgc acaacggatc agcaacattt
    10081 tttcaactgt tacagctatt tttattactt tatttactgg tccaattgtc gcttttttcc
    10141 ttttgagaga tcctcaaaaa ttttatcagt cagttttagc gattgtccca ccagcatttc
    10201 gtacagattt taaaaatttg acaaagattg ctaatcaaca attgggttca tttctaaagg
    10261 ggcaaatcat tgcttcattt atccttggag cagtctactg ggtttgtttc ttactgatcg
    10321 gattagaatt tgctagtgtt ttagctattt ctgctgggtt gttatgtatc attccatata
    10381 ttgggccgtt tatcgccttc tttccaggtt tgatcattgc attccaagac tctactttta
    10441 tggctgtcaa atttgtcatt gtctggtttg ccgtacaact tctccacggt gacttggtga
    10501 ttcctcgagt aatgggtgat cgtttacaga tccatccgat tacgatctta atcgttttac
    10561 ttgtcatggg tgatctaatg ggaattgtgg gcgttatttt tggtattcct atctatacgt
    10621 tagtcaaatt actcgttact tttattttcc gtaaattcaa acaacgctac aataaatttt
    10681 atggcgataa aggagagtat gaaaactctt cattttctga agaagattat tttaaataaa
    10741 acacaaaacg tcgatcttac gagaatagct cgtaggaacg acgttttgtg tttttgtttt
    10801 aatttttcag caaagcaatt gcctggtatg gttttagctc cagactttga tgaagaacag
    10861 tatcggaata attatcgata agaatattgg cttctgtgaa tccttctggg atggcgatcc
    10921 ttgtatcttt atcatagaaa tttgttaata caagcagctg ctgtccttca aattggcgaa
    10981 gaaagccata aacttccgga tgatttgctt cataagcatg ataatctcct tgtgcaacga
    11041 ttggatatgt ttttctcagc cgaatcaatt tcttgtaata ggacaaaata gaatcttcag
    11101 aagatagttc tttttctaca ttaatctctt ctagcttgcc tccaactttt aaccaaggtt
    11161 tccctgtagt aaatcctgct tgttctgtgt ctgaccattg catgggtgtt cgagagttat
    11221 cgcgagattt tgctttgatc cgacgaaacg cttctttttc tgtgaatcct tgttcaagga
    11281 gtaacttata agcattcaaa ctttctacgt caatatagtc ggaaatcgat gaatagtctg
    11341 ggtcgatcat gccgatttct tctcccatat aaatataggg cgtcccacgg ttcaaatgga
    11401 tcatggttgc tagcatctct gctccttgtt ttcgatactg atcgatagct acaaatcgat
    11461 tgagcgcacg tggctgatca tgattattaa aaaatagagc attccagccg ttaccctcac
    11521 tcatcttttc tccccatgta tgaaatagtc gctttaattc tgcaaaatca aatggaggag
    11581 tagaccattt attcccattt tcataatcga ctttcaaatg atggaaatta aagaccattg
    11641 ataattcttc tctactaggt tctgtataca agatacaatt ttcgatcgtc gtagagctca
    11701 tttcaccgac tgtgatgata gtggaatccg tcccaaacgt tgcttggttc aattctttta
    11761 gatacgtatg ggtgattggt ttgtccgtat attcaggttt gccatcattt tctttattat
    11821 ttttcaattg ttcgtcttta ccgataacat tgataacatc gaaacggaat ccttttaccc
    11881 ctttttccat ccaaaaacga acaactttga atagttcctc tcttactgct tggttccgcc
    11941 agttcaagtc agcttgtgtc cgatcatata agtgcaaata atatttcccc gtatctccaa
    12001 atggcgacca agcacttcca ccaaattttg atacccaatc cgttggctct tctcttaaaa
    12061 tgaagaagtc ctgataatgc tggtcccctg ctagtgcttt tttgaaccat tcatgttctg
    12121 tcgaaacgtg attcagcacc atatcgagca tgatttctat ccggtatttc tttgcttcgg
    12181 ctattagctg ttcgaattcc tccatcgtac caaagatagg atcgatggct gtgtaatcag
    12241 agatatcata accgttgtct ttttgagggc ttggataaat agggtttagc caaatcatgt
    12301 cgatgcctag atcattcaga tacgggagtt tttctttgat tccttttaaa tctccaattc
    12361 catcaccatt actgtctttg taagatttgg gatagatttg gtaaatgact ttgtctttaa
    12421 atgtcatgga tatttacctc cattcaactg atttattacc taccacaact tatatataca
    12481 agttatcatt tattattata actgaaccga aagcgtttgt aaaatttatc tttatacatt
    12541 ctaagaggtc tgttgcccaa taaaaaacaa gagatcagac atgtatttgt ctatcctctt
    12601 gtttaaaata gcaaaatatt cttgtttctt ctataaatgg tgttcaaaag cttttatcta
    12661 cggcaatcag tcttctcaaa tgtaaaacat gaggagctaa ttttatttga gtattctttt
    12721 tgctcgatgc aggacggctt ttttacaacc tctgattcat ggtgttcaat cagctgtcct
    12781 tcggtattag ctcaaaaaac ggcaaaatat gaaaaactat tttgccgttt gtttctctaa
    12841 tactcaggtc aaaccgctga tttcacaacc tctgttttta ttcattcgat ttcaacgcat
    12901 caatcatatc gatatgcttt aatttgaaat gggtaacaat catcacaata accgtaaata
    12961 cgatagttaa tcctgctgat aagacataag cccaccatgt gatcactaat ggaaagatga
    13021 cattctccat agacgcttgt tgcaagatga agtcagtcaa tatatatcct acaccaaatc
    13081 ctccgatgat tcctaaaaga gtaaagatga tattttctcg aacaatgtac atcgtgactt
    13141 ctttatcaaa aaatccaagg acttttattg tagataattc gcgtacacgc tcggaaatat
    13201 tgatattagt tagattatac aagacgataa aagccagcag tcctgataat acgacaaaga
    13261 ttaagacaac cgagtctaag ttcgccattg attcttcttg cgtttcgatt tgtttggaca
    13321 tgaaagacgt atttgtcaca gcgtctgtgg caagcagttt ctcggaaagc atttcttctt
    13381 cttttgttgt catcttatct gtacgcagca aaaatgcatt atccatcatc gattgatcag
    13441 taactgaagc ataatattca ggggacatat aaataaagct acctagatag tttttcacga
    13501 tttcttttac ttcgactttc cattcttttt gatcttcatc ataaagcgaa atatgatctc
    13561 ctgtagataa gtcaaatagt tctgctactt tttgcgtaat agcgattcct gttttaggca
    13621 aggagcgtgg gttctcttca agatcagtta aatggataaa acgatccaaa tcagctgttt
    13681 tacttggtac gatcactgtc agactttgcg aagcatgtcc tttttttctc aattctacgg
    13741 tttgagtatg aatagctagg atctcattta ctttttcttg cttctgcagc atatccgctg
    13801 ctttttgacg actatcttcc gtggatgaat ccaatgtaac gattgcttga tagtcaatga
    13861 tcgttccaaa ttgtttggct gaaacagaac ttagagaatc tttcaatccc acgcctgcaa
    13921 ccatcagccc ggcacatcca gcaattccga taatcgccat aagcattctt gacttatatc
    13981 ggaagagatt tcggtaactg attttttgat taaagctaag gcgtgaccac agcggagtga
    14041 tgtattcaag aaaaatgcgt ttccctggct tcggtgcttt cggttgaagc aatgcagcag
    14101 gccgttctct taaatctttg aataaaacga tcattgcagc gccaaatgcg gcaatgaaga
    14161 atgcggcagc tgcttgtaaa atcggcggcc agtcataaaa aataagcgca ctcccaatat
    14221 cataccgctc gttagacagc tcaaaaatga tccttggcaa aagattcgtt cctaatatcg
    14281 ttcctaaaac aatacctatc cctgaggcta gacttgcata gattgcatat ttttttgcga
    14341 tttcaacttt cgtataacct aacgccttta atgtcccgat ttccctgcgg ttttcttcta
    14401 ccatacgtgt cattgtcgta aaggtgatca atgcggcaat aaagaaaaag aaaacaggaa
    14461 aaacattggc aatcgcagcg atacgatcag acagatcacc aaattcttga aaaccgacat
    14521 tttctgaacg ttcattgtat aggtatgatg gtttagttaa attttcaatt ttttctgcat
    14581 ttcgattcaa ttcttcctct gcagaggcca gttcacgttc tttctcaact aattgccttt
    14641 cttgttcagc aagttgagct tcttgttgag ataatgtttg aacagcgatt gttcgttgcg
    14701 cttcaggaag ctgctggata gttgctgact gttgcgctaa ctgtttttta gcatcttcta
    14761 accgtgttct tgcttgcgtt gtttgtgctt tgccatccgc tattttttgc tgtgccggtt
    14821 ctaattcttc gttggccgat tttttcagct cttctaagcg ttcttcgggt cgatcttgta
    14881 aatacttttc tgccttttct tgattttgct ccattttatc tttgtaagct ttgctgtacg
    14941 tattcaaatt ctttacgttt gtaaaatcaa gatataagac agaataaata tctgttttaa
    15001 aaacggattc aggtaaatag acaaaataat cgacgctacc atttccgacg ttcgcgtatc
    15061 ctctctccaa attgttgatg aacaatggag aacggacaaa gcccgtgatg gtatactctt
    15121 tttctttcaa ttcatctgaa tcaatctgat aaatatcacc gattttatac ccttcatctt
    15181 ccgctttttc atccaataca agctggtttg gtttttccgg gagttttcca tcagttaatt
    15241 gtagcttgtt ctgcttgtta tttttgttat aagaaaatat ttgaatcact tcgtttttgc
    15301 cagtattgga ataatagatc tgttttcctg tttctacttt tgcatctggg atcttttctg
    15361 cttgagcaat atctttttgt gtcaagccac cagtagaaac gatttgcaaa tcagaaagtc
    15421 ctgctgtacc aacatagtga tccatcgttt tttgcatgac aggtccagca gttttgacac
    15481 ctacaaataa aagtgttcct agcatgacaa taaggatgat tgcaatgaat cgtcctttag
    15541 attgccgaat ttcgcgaaaa cctgctttaa gatatgttct tgttttcata aaatccccct
    15601 accatacaat ctcatcaatg gatgaaggat gatcatttaa ttcaacagac cttactttag
    15661 catcattaat gtgaatgact ctatcagcga tgggcgctaa agcagaatta tgtgtaatga
    15721 tcaaaacggt attgccgtgt tgtctgctag cgttttgcaa taatttcaat acttgcttgc
    15781 ccgtttcaaa atccagcgcg cctgtcggtt catcacataa aagaagtttg ggatttttag
    15841 ctagtgcacg agcaattgaa accctctgct gttcgcctcc tgataattgg gacggaaagt
    15901 tattcaatcg atgagctagg cccacttgcc tcaatacctc tacaggatca agcgcatcag
    15961 gagaaacttc cgttgccaat tccacatttt cttttgctgt tagattagga acgagattgt
    16021 agaattggaa aacgaaccct acatctgttc gtcggtaagc agtaagctgt ttatctgaaa
    16081 attgcgcaat atctgtgtca tcaataatga tctgaccttc atcaggtgta tccatcccac
    16141 ctaaaatatt aagaatcgtt gactttcctg ctccacttgg accaagtata atcactaatt
    16201 cgcctttttc aattgaaaaa gaaatatcgt catttgctgt aatggtcgtt tctcccattt
    16261 tgtatcgttt gtattcattt tttacttcga catacttcac tctgattcct ccattttcac
    16321 gatatttctc gctatatcta tgcatcgaat gcgttttctc acacttattt attttaccac
    16381 tgaatttctc tagactaaag aaaggaatcc tttaatttag ttgacaaata aacgatttat
    16441 gatttactta cataaaaaac attgttattt gaaagaagga acatcatgaa tgatttaaag
    16501 gaaaatatga tttttgattt atcaaaaact gcttttgtcg cgatcgacat gcaaaaagag
    16561 atcgtaaata acggtacact ctctccacac actgcagcat ctattctgac aaacaatgat
    16621 ttgttagtaa gaacattgaa acgtaccgct gccttgaaag ttttagtgaa cgtagacatc
    16681 tccactttcc cttatttgag tcaacaaact gatatgggtg gaatgactgc ttctgtacct
    16741 ccagaattca ctgatttact tttgaaggat tctttgaaag atacagataa tatgttgact
    16801 atcacaaaat acaatccaag tgcttttttt ggtacttctc ttgatcttca acttcgccgg
    16861 cgtggaatcg aaactatcat tttaagcggt gtagcgacaa caaacggtgt atatgctact
    16921 gcattagatg cctttcaaca cgggtaccat atcgtgcttg ctgaagatgc ttgcagtgat
    16981 cgtgacaaag aatcccatca attgttcata aaaaagatat ttccgaaaac tgcacgagta
    17041 cgctcaacga aacaaataat cgaagctatc caacaaagca aataagtact ggcacacgtg
    17101 attttcattt acttcttttt gatttcatgg gaaaataacc acaggattta gataggagta
    17161 tgaacatggt gaaaaaaaag atcacaatca aagaagtcgc taaactttcc ggcgtttcaa
    17221 tcacaactgt ctccttgata ttaaatggaa atacggagaa attcagtccg aaaactgttc
    17281 agaaagtttt agctgccaaa gaagaattag gctatcagcc tgactatttt gctagacaaa
    17341 tgattaccaa ggaaacgaaa acaattggtg tactcgttcc tgatatcacg aatccattct
    17401 tcagtacact gatgcgagga attgaggata tcttgtataa gcagaatttc gtcacgatat
    17461 tatgtaatgc tgattctgat catcaaaaag aaatcgaata tctagccgag ctcacacgtc
    17521 gaggtgtgga tggattcatt atcgcgactt ctgctgtctc tacagatgca atcaacgaaa
    17581 atttgaaaaa acaaggccgg cccttcattg ttttggatca gaaaaaatct gaaggattta
    17641 gtgatgccgt acgaacagac gattttcgcg gtggttacct agcgggcatg cacttgctta
    17701 gtctaggaca tcaaacgatc gcccttgtct atcctgaaaa tccaccggaa aatgtccacg
    17761 ctcggattga ggggtttaaa tctgctcttg atgtttacca aatccctcac gaccaactga
    17821 tcctcttacc tacacagttc tcaaaacaag gtggctacca gatcacagca gaacttcttg
    17881 attctgctgc aactggtgtg tttgctttaa atgatgaatt agcttttgga ttatatcgcg
    17941 gattagagga agctggaaaa agcatccctg aagactacag cattattggc tatgataata
    18001 tagatatgtg cgagtatatc aagccgaaac tgacaacgat cgcacaacca atcttcgaat
    18061 tgggacaaac atctgccaag ttattgttag atcggatcca attcccagaa aaagaatggg
    18121 aagaaaaaag attacctgta cgattagaaa aaagattttc aactgcaccc ttgaaatagc
    18181 tttcaaggtg tgctatattt ttatcgaact attaaaacgt tttactaaaa cgttttaaaa
    18241 aataattttc ccatcaaaat aaatgagata gttggaggtt tgtaaaatga acacggttac
    18301 agtaattgga agtattaata tggacactac ccttcgttta accaacatgc ccaagccagg
    18361 cgaaacgatg catgcgcatg agatttttca cgctggcgga ggtaaaggtg ccaaccaagc
    18421 agtggcagcc aaacgttcag gcgcacggac atcttttatt ggaggcgttg gggctgacag
    18481 cgaaggacaa cagttattag atctactgac aaaagaaaac atcgatacat ctgggatagc
    18541 tgaaatccaa ggcgccacaa ccggacaagc aatgattatg gtagacgcag caggagaaaa
    18601 tagtatcttg atccatgcag gggcaaataa tgctttccat gaacaagaag tcctaaaaaa
    18661 caagcaactg attacaaaca gtgactttat tattgctcag tttgaaagta gcttagatgc
    18721 gacgatccta gctttttcta ttgcaaaaga cgcaggaaaa acaacgatct tgaatcctgc
    18781 accagctcgt gaaacgattc ctactgaact acttgaaaag acagatatca ttataccaaa
    18841 tgaaacagaa acagaaatca ttacaggaat cagagtgaca gatcataaca gtttggtcgc
    18901 agcagccgaa aaactccatg aattaggtat tggaacagta atcattacac taggcagtgc
    18961 cggcgctttc tatcatacag aaaaagaaca cggtatcgtc cctgctttta aagtggatgc
    19021 cgttgacaca acagccgcag gtgatacatt tattggcgca ttaagcagca cacttcagcc
    19081 agaccttagt aacttgaaag aagcgatcct ttatggaaat ctagcctctt ctgttgctgt
    19141 gcagtcttat ggtgcgcaac cttctatccc ttaccgcgaa gctttagaac aatcagcagt
    19201 ctaaaattaa caatgagagg aagaaacaaa catgaaaaaa ggaaaagtaa tcaattcaga
    19261 tatttcacga gtaatcgcac aaatggggca ctttgataaa ttaagtatcg gggatgcagg
    19321 catgcctgtc ccctttggta ctgaaaaaat agatttggct gtctcaaacg gtcttccaag
    19381 ttttttatct gttttagaaa acgttttaga agaacttgag gtacaatcga ttgaattggc
    19441 ggaagaaata aagacaatga acccggaaat attggaaaaa atcaaattat tgttaccaga
    19501 tacaccgatt agctttatcc cacatgaaga aatgaaaaaa gagttgaata actgtcatgc
    19561 atttattcgt acaggcgaaa tgacacctta cgccaatatt atattagtga gtggagtggt
    19621 cttctaatgg aaacgacaat gaatcctgaa cagatggtac tgtccgagca gattcatatg
    19681 ctgattgaac agttgggagg aaaagacaat attgattctg tccttccatg tgcgactcgg
    19741 cttcgtatca aattgaaaaa acagccagta gccaatgcag aaaaattaaa acagcaaatc
    19801 ccttccatta agtatttcat tttactcgat agatatgcag aaataatcat ggatcaggga
    19861 ttggctcctg cttacaagga attgacgcgt caaatcgatc attcagctcc gaaagaaaag
    19921 ctaaatccga tcgaaaaact atttggtttg atatcggaaa tctttacgcc gatcattccg
    19981 ctgtttgccg gttctggtat attaaaaggt ctggttgttt tagcgactac gattggctgg
    20041 cttagtgaaa caagcggaac ttatcatatt ttgtctgctg cgtcgaatgc cgtcttttat
    20101 tttctgccaa tctttcttgc tttttctgct gccaaaacgt ttaaagtgaa tggatatatt
    20161 gcggctgtca ttgctgcagc actgattgaa ccgaatctga cctctttact ttcagacagt
    20221 ggcaaagcag ctactgattt tctggggatt cctgtagtac tgatgcagta tacgtctact
    20281 gtcatgccgg cgattttagg tattttcttt tattcttttg ttgaaaaatt cctcaaaaaa
    20341 tatattcgcg aaaatatgca attagtattt gtacctttac tttcattagt tatcacggtt
    20401 cctttgacgt taatggtatt cgggcctgcg ggtgtttacc ttggcgaagg tttagctgcc
    20461 gcaattactt ggatgatgga tattaacgga ccgatcagtg gcgcaatcat cgctggtggt
    20521 tggaatattt tggtaatatt tggtatccaa tgggctgtaa accctgtcat gatcagtaat
    20581 atctctgctt atgggttcga ccgaatcgtt ccgttgacag gagcagccaa ttttggaatg
    20641 gctggtgcag ctttaggtgt atttctgcga agtaaacgtt caaaaacccg ttcgatctcc
    20701 ggttctgctt ttgcttcgat tttacttgcg ggagtgaccg aaccaactgt ttacggaatt
    20761 gcgatcccat tgaaaaaacc atttgttgct gcttgcattg gtgcagctgc tggtggagct
    20821 gtaatgggat ttgcccaagt gaaagcgatc gcttttgtat ttggtagtct gaccacattg
    20881 cctgcattca tttccggtac ttttttctgg tacttagcag gactggctgt tagtctagtc
    20941 gtagcaatga tcactactct cgttagcggg tttgatgaag acctaatgtc ttatgaatag
    21001 ttaatgaaaa agactgaagt atgagatgtt tatcacatat ttcagtcttt ttctttttag
    21061 gatcattatt tttataaatt tttgtgatac tttccaaact ttggtataat ttacatatgc
    21121 ataaccattt ttatccccac ttgtttatcc ccaaacaaag gtggtctatg caataaaatg
    21181 aacgtcaaag aaaaacgaat gaatgctcgt tgcttattta caaattgttt tatttagaat
    21241 acacagacag atttatgaaa agaaaaagct tttcgacacg attatctgtt ccaaaaaatg
    21301 tctaatgaat gctgaataag acagaaaatc aatgaaagga aaaggactag tttattatga
    21361 aaaataaaaa aacgatacgt ttgctagctg ctacttggct gattgctccg tctgttttgt
    21421 cgaccaatac cgtatttgcc gtagataatc aaatagctga tcaaacagtc acaacagata
    21481 cgactacatc cagctcagct actagtgaaa caacggattc ttttacagaa gacccaactg
    21541 gaacaactaa ctcaacaaca ggatcgaccg agccaactga aattgatccg gctgatccat
    21601 cagaaaatga aaagccagcc gagacatatt cccttacaat tgctccttct ttgattggta
    21661 agatccaact gactgcttcg acagcggatt cccctgaaca gcaacctttg gatattacgg
    21721 aaagtggaac ggtagaaggt ttattagcgg aaacgaaggt aacttacact attacgccag
    21781 ctgaaggtga acaaattgct tcagtcgctg ttaatggtgc caaagaagta aattatgatg
    21841 caaagacctt tacagtaggt acgaacaatt tatctattac tgcagaattt aaagctggac
    21901 cggatacttc aacagagaaa ccaactgatc ctagtgaaga agtccctagt acaccagatc
    21961 cggaaacgcc aaacaaacca gatgtgggta ataacaacaa taataatggt tcaaataatt
    22021 caaacaataa taacaataat tcgaacagcg ggaattcttc gaatcaaaca aacccatctg
    22081 gatcgaataa caatggaaat actcagagag aaccaaacca tccaaacaat ggttcatcga
    22141 atggcggaac gaacaatgct tcatctcgtc caaatacagc aaatcaaagc ggtatagaga
    22201 atccatcttc tgcttcttct gattttgttg taaaatcacc aattgatgct gtattgccta
    22261 caaatacaac gaatgtccaa caagcaatcg ttcgagaagc attcaagcat cttggaaaac
    22321 catatgtatg gggagcaaaa ggtccaaata cttttgactg ttctggtttg acttattacg
    22381 tatatatgaa agcaaccggt cattatatcg gtggctggac aggcgaacag caatatgcag
    22441 gtacgcagat tccagtaagt caggcccaac ctggcgacct cgtcttctgg ggaccttctt
    22501 ccggtgttac tcaccatgta ggtatctata ttgggaacgg acaatttatc catgcgcctc
    22561 aaccaggaga taaagttcga gtaacttcta tttcagattt cacgcctgat tttgctgtcc
    22621 gagtaaactt agcaggttta cctgctgctt caggttcgct gacaggcggc tcatctattt
    22681 tagatggatt agatggctcg ttccatttca gtcaaaacca aacaactgat cagttcttga
    22741 aaaagattgc agatgatgca caggaaatcg ggcaaaaaga aggaatctac gcatcagtca
    22801 tgatggctca agctatcctt gaaagtggat ctggaaacag cttattatca agcgaaccaa
    22861 atcataacct ttttggtatc aaaggatcat ataaaggaag cagcgtgacc ttcaatacac
    22921 ttgaacaaga tagttccgga caaagctacc aaatccgcgc acaattccgt aaatatcctt
    22981 cttacaagga aagtttggaa gactatgccg acttgatcaa aaatggactg actggcaatc
    23041 ctgatttcta caaaccaact tggaagtcgg aaacaaaaga ctacaaagaa gcaaccaaat
    23101 atttggaagg acgttatgct acagatcgcc aatattcaca aaaattgaac gcgatcatcg
    23161 aagcgtacga cctgacaaaa tatgatgaac cgaaaaaaga agcagcaaca actgaagaag
    23221 caaacgctga aatcctcaat atgtctaagc gattcgatgt gccaatcaaa tggacaacag
    23281 atggcgtgcc aatcacagga ttagaatttt caacgataaa acgcgtcaac aacgcttctt
    23341 ccatcttccg tgtcgcttca atctgggatt tgtggaacaa ttttacggca agacagatac
    23401 cagaaacaac tactcgtaca gttaaagctg caactgttcc tgtactaacc ttgttaagtt
    23461 tagacaaatc attctggctt gatctgcctt taaagaaata gaataaatta aaaaggggat
    23521 gtgacactgt cacactccct ttttaattta ttctatttct ttatatttat tttgtattcc
    23581 gttacaaact cgcgtacttg gttaggttcc agagtgacat ctccaaaact ttcaagtcgc
    23641 tccgctcctg gcagttcttg agtctcaaat gtgataccgc catgatttac taatttattc
    23701 ccgcgcattt ccggtccatc ttctccaaaa tttgctgtaa aaatcacgat tcctgaagca
    23761 tctgttttta cttggatcga tactttttca tctgggcttg tcaattccgc agcatttagt
    23821 aaaccagatt gttttttcaa caaaaatgga tgatcgatgc cgcccactat cattgattgg
    23881 cgaaaatcac tggcaaagac ctcacctaat tttttaggac gttggaagtc aaatgctgtg
    23941 ccacttactg aaactacttc tcctgtaggc gttgtatctt tattgagcgt agcaaataat
    24001 cggctattca accaaagtat atgttcgtcg attgactgtg ctggatcacc ggttaaattg
    24061 aaatagacat gatttgtagg attgaatact gttttgcggt cactgatccc ttggaccgtc
    24121 actcgccatg tatcatcgtt cgtcagcgta taccgtactt ctacatctaa agttccagga
    24181 aatccgtttt ccatatctgg gctagtctga tagaaaatga ccgatgcttc gttttcacca
    24241 ttgatgactg tataatgcca ctgcttggct tcaaagctag gtgcaccacc atgtaaacaa
    24301 tgaccagtgt ccgggtctgt ttttacttcg aacgtttcat catctaattt gaatgtgcca
    24361 ttcgtaatcc gtccagccat acgtccgatt gaagcaccga tataagggtc tttttctaga
    24421 tattcttttg cttcatcaaa acctaagatc aactctcgtt tttcattatc gattattgtt
    24481 tgaaaatgaa caacccgtgc cccaagatca ctgacagaca aagatgtccc attttgattc
    24541 gtgatcgtaa tcaagtgata gttttcgcca aaattttgtt cattgatttc catgttcctt
    24601 gcaccctctt ttctttcatt tgcactgatt ccagcataca tggaaaccgt tttcttaaca
    24661 gcttattccg tgagtaacga gaaaaaattt actatattct aataaaagac cgtgaaaaaa
    24721 aggttgtgac aaaaatattg tcacaacctc tttttccgaa caaacggtgt tcaaaagctt
    24781 gcatctgcgg taataagccc aaccaaacga aaaatatgag gagctatttt tgcttggctg
    24841 ttcttattac ttgatgcagg acagcttttt cacaatctct ttttcaatac gattccagta
    24901 tataaccatg tcgtcagtac atagtagatg aaatacaata cgaagaatat cgtaaacgga
    24961 acccaaatat ctttgtatgg attttgcatg aatagaccaa acatttgtaa tccgaataag
    25021 acatggacta cacctaagat tcctggtgct aagaataaca cgcctacttc tctacggatc
    25081 gattgtttaa gcaacccgcg tcgtgttcct atcttttcaa gcatttcata acgagcaatg
    25141 tcactcttgc ttcctgacaa gatcttgaac atcaaacagc tagcaagcat ggtcaagaaa
    25201 gctaaaccta ggaagaatcc cataaattca aaaccagaga atagaccatt atacacttca
    25261 tacatttcat atttctggtt gctgtactct actttttgca aagatggatt gttcatttta
    25321 ttttgagcaa caagtgtttc gattggtttc aggtttgctt ggaagtcttt tacctgaact
    25381 agctgtaaag tggattgtgg caagttcagt tgatcaaatt ctgattgact gactaccttg
    25441 atttcttttt cttgctgttc cggcaattca tagcttcgaa gttcctcttg tacagtcagt
    25501 tcattcattt ttttagcagg aactttttct ttcgtgatct tcggcggtgt tccatcttca
    25561 tgttttatca tgatcaatgg ttgagccgca aattcacttg cattatagta aatcgttccc
    25621 gcatcttctt tttgagtata agaagcagaa agtgttggtg aaagttcttt gattttttgc
    25681 tgatcttctg cccgaggatt attcatcact agatcatatc cagaattgtt ttctgtcaag
    25741 tctgcaattt catttttaaa acctaatcct accgtcaaag cgcccagtgc caaggcaaac
    25801 agcatagcga ccattgagag catttgtgta tattcacgaa tacgaaagtt taactgtgac
    25861 aatgtgaaat tgttcaattt tttcaatgaa atctgttctg atttctttag taatgataaa
    25921 aagaaaatga tcacagaatg gaaaatgaaa taagttccta atacgatcgt aactaaggct
    25981 acccctatac caaagacttg ataggtcccc acgtgtttca tcatatagta accgcagcct
    26041 agaaaagcaa ttcccagtac tacttcaagg aaaaacaaga atttatgctg cttgattctt
    26101 gttggtgtct gatccgcacg aatcaatgct aaaatcggtt ttttgacaat cgaaaaagca
    26161 ttgacaacag cagctaataa aaacaaaaca gcaaaaaaca aaacagtgat caacaacccg
    26221 tgaatactaa aaggtgtaaa atgcgaaata ttgagatcta actgatccac taataatcgg
    26281 tggacgatac ttgtcaatcc tacaccgaca gctgaaccca ctaaagtagc tgcgatccct
    26341 actaagaatg tttcagaaaa aatcatctgt gcgatttttc tgccttttgc tcccagcatc
    26401 ataaacattg catagtcttt ttgccgcatc gtcatcaaaa aggaatttgc atataaaata
    26461 taaacgaatg tgatgatccc tagtaaaaca gaaccgaatc ggaaaatgat aactgtagcc
    26521 gcaacgatcg tattactttt caaaaaagct tcgttgcttg ccattgattc aaacatgtaa
    26581 aaaatagcag atgccataac taatcctgag aataacacca agtagtctct taaacggctc
    26641 ttgatcccag taagtgataa tttccaaagc atagtcaatc tccccctact gttccaagtt
    26701 tccaagaata gatagtattt ctcgatagaa ggaatcacgg ctttgatctc cacgtttcac
    26761 ttcttgatgg ataccgccat ccttgatgaa taaaatacgt tggcaataac ttgcagaaaa
    26821 cggatcatgc gtcacaagaa gaatcgatac atgatctttt gtattcaatt cgtccatcgt
    26881 atctaaaaga tcccgggcac ttttggaatc cagcgcacct gtaggctcat cccctaatag
    26941 aatcgttggt tgtgtgatca atgcacgtgc ggcggcgacc cgttgttttt gtccaccgga
    27001 aatctcagaa ggatagcttt ccaaaatatg agagatggat aaacgttctg caattttttg
    27061 gacacgttgc ttgatttctt ttggtctgac gccttgtaaa gaaagaggaa cagcgatatt
    27121 ttcttgtgct gtcaagcttt ctaataaatt gaaatcttgg aaaataaagc caatctcatt
    27181 cgcacgaaaa tctgctagtt gatttccttt taatgtggtc acatcttttt gattgattcg
    27241 gatatgccca tctgttggtt tatctaatgt ggataaaata ttcaaaagtg tcgacttacc
    27301 agaaccagaa gcgcccatga tcccaataaa ttcgcctttt tctacatcga agctgatgcc
    27361 gcttaatgct tgcgtctttt tctcattgtt ttttccgtat acttttgtta cattctgtac
    27421 ttcgactatt ttgttcattt ctttccctac cctttaatta gtttttccag tgctgttttc
    27481 ggcacgtctt ttttttcgac ttcttcatac gaagtgacac ctttattttt gttccaggtg
    27541 atttttaaat atgctccttt acgaagcggt cgagtcaatt gtcccttgaa gtccagttct
    27601 ttttccttac cctctttcgt atagccaggc aggatatatc tatagttgac tgtttcttgt
    27661 ccagagtctg attttgaaac tgtttttgta ccatctgtcg taatttgtac atagtattct
    27721 tctccaccca taactacgta atctgcgatt ttcacccctg caagtaccac aacgatgaaa
    27781 acaagtagac cgatcagaag ttttttcact ttttcccctt ctctcttact ttatatactg
    27841 attatagaaa aaagcgaaca attctgcctt cgaatcaact atcattcttc tttcaatttt
    27901 gtcattttct ttaaaacaac taaaaaaaga gttcaagaca ataatccgtc ctaaactcct
    27961 tcttcctatc ttacaaaaag tatgtcactt acggtctttc atgtcgcctg tattgataac
    28021 aggttgcgtt cctttgtctg taatgattga gacaagtaaa ttattgatca attggacttt
    28081 ccgatcatcg gtgaaattga tttcttgtcc atcttcgatt tgctctaagg ccatttgagt
    28141 gattgtgaca gctccttcaa caatggtttg tcgagcagac aaaatggctt ttgcttgttg
    28201 acgttggagc atggcactag cgatttctgt tgcataggct aaatggttca agcgtgtttc
    28261 gattacttcg acacctgcta ctgctaaacg ctcttgcagc tctttttgca actcgtcaga
    28321 cactgccgtc gtgtttcctc gaagcgtcaa atcattctca ttgaatgtat catacgggta
    28381 ttggctagcg atatgccgaa tggctgtctc actttggatt tctacaaaat cttgataata
    28441 cgctacatcg aataatgcct ttgctgtatc aacgacttta aagactacta ctgctgagat
    28501 ttcgatgggg tttccatcca aatcattgac ttttaaaaca gaactattga aattccgaac
    28561 tttcaacgaa acagtcattt tttgtgccaa aggtatcgta aggaaaaagc cattttctcg
    28621 aatcgtacct aaatattgtc caaaaaacaa aattactttt gcttgattag gactgactac
    28681 agttgccgaa cttaacaaca ataacgtgat caaccaaaga aaaatactta aaaaaagtgc
    28741 ccaaactttc gcttgccaca tacctaaata gaagaaaaac aatccaacta aagctaatac
    28801 gaccaatccg acaatccctg cataaccatt gatataaaat gctctttttt ctctcatgct
    28861 acttcctgcg ctcctttatc cttcaataaa tatctaatgt tactttttta attttttttt
    28921 cgaatacgcg ccattacacc ttctagttct aactctttgt cttcagtgat gtaccaaccg
    28981 cttgcctgtg tccctccggc tgcctgcggt aaataatcca attttgttcg aacccagtgc
    29041 ttttcttctg taaaatagtc ttctaccaag acctctagat ccatccctgc tgaaagaggc
    29101 aaatcttccc gaacagattt aaagataaat cgttccttct tcccatcata cttcaatatt
    29161 cctgtatcga taatcggtaa agaataataa tccagctctt tcttcaccgt caataaatgt
    29221 ttgaaaactt catggtacac ttgctctaat tggcgacctt ctgaattgtt cgggtaatta
    29281 accatcactt tttctttgtc tggatcatag aaggtttggc ttagttgagt gatataagta
    29341 tgtaatcttt ccatttctgt ttttaacttt tctctttgac cttcttctac cttcatatac
    29401 tctttcaccc tgttttcttt ttagatattt tctttcttat tttactatag ttatgtgcgt
    29461 taagcgatga aaaacagcaa agattggaag gatctttgct gttttttctt aaaatatcca
    29521 gttgagtaca ggatcaatct gtttcattgt cctcttgatc cgtcgtaata ttgatcgatg
    29581 tgtctttagc agtgatcttt agctttttct gtccttgttc taacaaagta tttgttattg
    29641 gctggtagtc tttttcaata taagattcac cagacgtttg gatagtatag ccactgtttt
    29701 ccatctcatg caaagataaa ttgccaccgt ttaggatgac ttttccttct ttgctgatca
    29761 aactgtcttc tagatggaag ttgctgtttt cacctgtgac gatcaagtta tccacattgg
    29821 cctcatctaa tcgaagtgag cctaattcat ctttcgcagt tattgttcct cctgtaaatt
    29881 cattgaacga tacccagcca tcatgtgtct tagtattcac ttttttgatt gatacttcgt
    29941 tgatatcaat tgcactatta ttcccttcga ttgtcaattc agataattca tgatctgcag
    30001 ggacaacaat agtgatttgt gcacttgtgt cttctaaact aatgattgca cctgttgatt
    30061 ttttgactgc attcacagtc aacacgccat ttttctcctt tatttctggc ttttctgtct
    30121 tatcataagt cgtttttatc tcaaaatcca atccgcgtat gaccgaaact gtttgatttt
    30181 ctgcatggat gacgattttg tttgcatcct tgattttttt tgtctcagta accatatcga
    30241 tcaaccgagg accattatcc caagtcagac tcttttgagc gcctaatgta taagttgcac
    30301 ctgctaggag gccaccagta agcatcagac caataccggt aatcaaaaaa tgtttcaatc
    30361 tcaatttatg tgtcctcctt caaacgcccg ctgaatgcag atctcttttt gtaataaaac
    30421 gtcgtcccat ccatttaaca aagttcatca tgacataaaa aagccctttt gttcctttca
    30481 gcaacagtgg cgaaataaat actgcgcttc ctgtggcgaa cagaccgaca ccgataaaga
    30541 ataatccact cagaatcgat tgtccaaaca cactgattcc tgagatgatc atgaataggc
    30601 caacacctaa gatactgata cataaaacat agatactaaa aatgagcgct agtaaagcaa
    30661 ccaatagtcc cccaataacc aatataaacc ccagtgctag tggaatgagg acaggagaag
    30721 caaataaacc gataatgatt aaccaaatca ggcgaatttg tctttttgga tttttcacta
    30781 actcgttttc tgcttgttct tcttctgaaa agtaactgat ttttaatttt aaggcaaact
    30841 gctttggtgt accatattca tcaatgatct gatctaatgt ttttccagaa tctaaaaaat
    30901 attcctcata atactggatc aaatcaaaac gatcgtcttc aggaatttct tgaagatgat
    30961 ccacaacttc attcaaatat tcattcattt gtgctctcct ccaataaccg gtctaaagaa
    31021 tctcgaaaaa caatccattc atcaataatt tcttctaaat gacgacttcc cattggtgtc
    31081 aacgagtaat atcgacggtt tctgccttca aaaggttcat cataggtctc aagatatcct
    31141 tcttttttca atcttctcaa aaccggatat agtgtagatt cactgacaga gagactttct
    31201 tgtacgattt ttgttaaggc atacccatac atatcttcct tgcttaatgc tgctagcaca
    31261 gtaccatcaa gaagaacagt tggaatctgg atgtgcatta cgtcaccttc tattctcaag
    31321 tgtaatattt tatttcaatc tatattatac aacatataat atagtttcaa aatcagtttg
    31381 tcaaatacaa aaaaactgac taaaagattt gatccttttt agtcagcaaa aaatggcttc
    31441 tctttgattt ttattataac agtggcataa tccaatttgt ccacatccag ctcagtggca
    31501 tgataacgat cattgcaggt atcatgtcag ctactgggaa cattttcacg ttgatcattc
    31561 taaaaccggt agcgaccatc aagaatccgc cgcaagcttt aaaatctaag atcatgtctg
    31621 gtgtagtcaa tggataaata aattttgcca ataagaacaa aatataaaaa ataatgaatt
    31681 ggggaattgc tatcacagaa acgacatacc ctaaattaca cgcaaaaata gctgctgtga
    31741 aaaaatcaag gacagattta gaaatcaaaa tcgtactgtc tcctgtcatc ccagaatcta
    31801 aacttccata gatccccgta ccacttgcac aaaatagaac aatgaccgtc actaatgtgc
    31861 tgataaattc atcgtgggtc ataccaagct tttcactagg gaagatttta gaaataggta
    31921 cttgcatcag cgcagccccc ttatttatcc cattacctaa atgaacaatc aaaccaatcg
    31981 ctgttcctat cactaaagca aaaataacag ccggcatatt tttcatcggc gcaatggaat
    32041 agattcccat ccccatcgaa cacacaccaa aaaccatcgt cagctgtgct ttaaaatgtt
    32101 cacttaattt atttcctact aatccgccaa ttagtccgcc aaacaaaacg gaacctacat
    32161 tgatcaaaac tccagttggc atcaaaaaac tcctctcttt ttctacgaag cgaaaaaaat
    32221 cagaaaaatt tctgtaatta ttgctttcgc tgcaagaaag attgtaacag acagagaaga
    32281 ttacaccaat tcattttttg tgttaaagta ttctttattg gaataagtag gaggaatatg
    32341 atggatatcc ataaattcga agtttttctt gatttagccg aaacaatgaa tttcaccaaa
    32401 actgctgatc gacaattcac cacccaagga aatatttcta aacagattct ttcgttagaa
    32461 acagaattag acgtcaaatt attcgaacgc gcccatagaa aaatcgaatt gaccgaagct
    32521 ggaagcttat tgctcccata tgtaaaaaat gtcgtagaac aataccatgc tatgcaagaa
    32581 gtgcttgaaa cctatacgaa agacaacaat ctttcattaa atatcttaac gataccgaca
    32641 atgatcaatt ataaaggttt ttccaaaatc accgagtttt tgaaacgcca tcctgaattt
    32701 acagtccagt tgaaagaagt ggaaagtgtc gaattaactt acaatgacgc caatgaacat
    32761 agtgacacga tccactttgc tagaagtttc caggccccac caagttcgat tgagtggttg
    32821 cctactgaaa cagacgattt tgtagcagtt ctacctaaaa atcatccttt agctagtaaa
    32881 aagaaattgg atctaagcga actgaaaaag gagaactttt tgctccttgg ccccactacg
    32941 aacttgtatc aacctgtact agatctgtgt cgcgaagctg gatttgaacc aaaaatctct
    33001 tataaaggag caagaatcga cttgatcatc aatatgattg caaatgacat ggggattgct
    33061 attgttatgg aaaaaacagt caagaatcta ttaaaagaaa atactgtgat tctccctatt
    33121 tctccaacaa cagagagtta tcttgcattt actcgtaaag tcggtgagca ttctttggct
    33181 tcagatacat tttgggagta tttaaatcaa gaagaaaact aagccctaca ttttctgtcc
    33241 atctatccac ccaaaaaaag agagcgacgc atgactttcg ctctctttct tgcatcatgt
    33301 ccttgtttca atgacacctc taacatttct cgtagttcaa taagaaagca gcgattttta
    33361 tgccaaaaga accgtaagaa catttcccta atcacaaaaa aacgactaaa aacccctaaa
    33421 aaccttcaat agaattttta ggggttttat tctttttcct attgtttact taaccgtatc
    33481 attgctgtcg ttcctactcc ttcgatactg ctataatcaa tacgtccatc atgcagttct
    33541 agaatttttt taaccagtga aagtcctagc ccattcccag aaatttgacg acttgtatct
    33601 ccttgataaa atttttcaaa cattcgatct ctggtctctt cattcattcc tacacctgaa
    33661 tcggtcactt ccagtacaat ctctgtggct gtctcaaata gtttgatatg aatctgtcca
    33721 ttgatttgat tgtactttat ggcgttatcc atgatattca gccacacttg gtacaaaaac
    33781 tcttcattgc ctgtgtaatt tactgcagca agttcgatat ccaattctag ctgttctttc
    33841 tcccatttag gctgaaaaaa aacgattact tctctgattt gttcatctaa tcgatactct
    33901 ttcttttcca gttggatacg ctggttttcc agtttgttca attttaagat gttctctgtt
    33961 aattgtgaaa gctgcgtgat actttcgata atccgatata aaaatatttg gcgttcttcg
    34021 tcagagagat ttggggcttg taacaattga acatatcctt ggattgttgc tagtggtgtt
    34081 ttgaattcat gggatacact ggacacaaag tcattcctca gtgtctcgat actattgagt
    34141 tcttggacca taacgttgaa atctttatac agttgctgta cttctgctac tttctgctgt
    34201 tcatccattc gaatcgaaaa atctcccgtt gctaccagac tcatatttgt ccctagcgca
    34261 ctgattggat gcaggatacg ccgcccaaca aaaatcgata ttcctgtacc cacgaccata
    34321 ctgaaccccg caaagacgaa taacgggtat aacgttggtt ttccagccga ttcagcaggc
    34381 gagatagaat gtcgagacaa taagaaaata aaaccgagaa aacacagcag tgtcactaag
    34441 atcgaaatga agacaatact agcaaaatag atccataatt gtgaaaatag cttttttttc
    34501 atctttaaat aaccgcctta tagcctagtc cccgtactgt gatgatttga aaatcttccg
    34561 tcttttcaaa acgggtcctt aaacgtttga tatgtacatc gatcgttcgt tcttctgtat
    34621 tcgtatctaa tccccaaata tcatccatca actgttggcg agtgaagatc ttgttgggat
    34681 aagacagtaa tttatacaga agcagaaatt ctttttgagg cagaatcaac gaatgctgat
    34741 cttgtaacac tgtataagca tctagctcta attttgtttg tccgattatc aagctactgc
    34801 tgtaattgat ctttgaacgt cgaagcaatg cttccacacg taggatcatc tcattgacat
    34861 cgatgggttt tgtcatataa tcatctgctc ctaatttgaa cccgcgtttc ttatcctcaa
    34921 aatcggcctt tgccgtaatc attagaacag gtgtgtcaaa tctggtgcta cgtaatgagt
    34981 ctagcaagtc atagccatcc attttcggca tcatgatatc tgtaatgatt aattctatac
    35041 gtgtcgcttc tagttttttc agcgcatcct ctccattcaa tgcggtgatg actgaaaagc
    35101 ccgctcgttc caacacagaa cggtaaagca actgtaattg cggatcatct tctactacta
    35161 aaatacgatt cattgatctc cctctgtttt ttgaaaaatt tctagatttt ttcttgtagt
    35221 tcatcttcag ttaacattta tttcgtatac tacgatcatt gttctgatat acttattagt
    35281 ataccgaata tttcaatacg ggagaagggt gattatgctt caattaaaag atataaaaaa
    35341 atattataaa gtcggcgaaa ccaccaccaa agcattggat ggtgtgtctg tcgctttcag
    35401 acaaaaagaa ttcgtagcga ttttaggtcc tagtggttct ggtaaaacga ctatgctgaa
    35461 tgtgattggt ggactagata attatgattc cggtgatatg gtaatcaacg gaaagtctac
    35521 taaagacttt aaggacagtg actgggatgc ctaccgaaac aactctatcg gtttcatttt
    35581 tcaaagttac aacttgattg gtcatctagg cattattgaa aacgttgaac ttgggatgac
    35641 attaagtggt gtttctaaag acgaaaaacg aaaacgggca gaagatgcat tgcaccgagt
    35701 cggcttgaca aatcatatgc ataaaaagcc aaatcagtta tctggtggac aaatgcagcg
    35761 agtggccatc gctcgagcac tagccaatga cccagatatc cttttgtgcg atgaaccaac
    35821 cggagcgtta gatacagaaa caagtatcca gatcatggaa ttgatccaag agttatcgaa
    35881 agaaaaatta gtgatcatgg tcacacataa tcctgaactg gcccatcaat acgcagatcg
    35941 aatcattgaa ttttctgatg gtaagatctt aacagattcc catcctcaca tcgaacgacc
    36001 aaaagatgat caatttaact tacgcagaac aaagatgagt ttttggacag cgttaaagct
    36061 ttccttcaac aatatccgaa caaaaaaagg acgtactttc ttgacctcct ttgcttccag
    36121 tattgggatc atcggaatcg cgatcgttct gtccctttct tctggattcc aaaaacaaat
    36181 cgataacacg caagctgaaa cgatggcgaa atttccgatt actatttcta aggtaacgac
    36241 taaccagact cgtgacgatg ccggattggg tgcaagcaaa gcggattatc cagacagcaa
    36301 aacgattact gcaaaagtca gcgaagaaga taaagcacag cacacgaata agattgatca
    36361 aacatacgtc gattacgtaa cagacatcga tcctaactta agtaataata tcggatttac
    36421 ccgtactaca ggaatcaatc ttttacgtga tgtaaatggc aaagtacagc ctgtcagctt
    36481 ctcgaatcaa aatcctgata cggaatctct ttctttatct agtacgatgt cagcaatgac
    36541 aggtgtggga gtgtcaagtt tccctacaca gcttgacact agcaaagaaa actttttaaa
    36601 agacaattat tctttacttg ccggaagtta tccagcctca gcaacagatg tcgtgctgat
    36661 cgtagacggc aataataata ctaatatcaa cgcattgaag aatcttggat ttgatgtgaa
    36721 agaagacgaa aaacttgatt ttgatgatat cgttggtaca acattcaaat tagtaaacaa
    36781 caatacttac tatacaaaac ttccaactgg gaattttatc cctaatacag attatgatgc
    36841 gatgtatcag aatgcttctg atgaattgaa gatctcgggg atcctacgcg tgaaatcttc
    36901 ttctacaatg aatctcttat ctccaggaat cgcttatagc gatcaattaa cgactcaaat
    36961 cgtcaacgaa aacaaagaat cagaaatcgt caaagctcaa agagacagtg gtgtaaatgt
    37021 cttaactact gaaaaagttg acgaaaatgc caaacaaaca ttgcttagtt atttaggtgg
    37081 agacagcttg ccttcaagta tcatgattta tccgaacaat tttgaagaca aagaaaagat
    37141 cttggattat ctagatgatt acaataaagg caaatcagat gaagataaaa tcatttatac
    37201 tgatttagct ggaacgatga ctgaactgac tggcggattg atggatgcta tcacttatgt
    37261 ccttattgct tttgcaggga tctctttagt aacaagtatg atcatgatca gtatcattac
    37321 ttatacatct gtgattgaac ggacaaaaga aatcggtgta ctaaaagctt taggtgcacg
    37381 taaaaaagac atcactcgcg tatttgacgc agaaacgtgt attttaggta tttcttctgg
    37441 gatattaggt gtgtttattg catggctagc tacgttccct atcaatagca tcttatacaa
    37501 catgacggat ctaaaaaatg ttgctcaact aaatcctgtc catgcgatta ttctagtgat
    37561 cgtctctact atattgacga tgcttggcgg tcatcttcct gcacgaatgg cagcgaaaaa
    37621 agacgccgct atcgctttgc gtgcagaata aggttgtgac aaccacattt agatttgagt
    37681 cataagagcg ctgaaatagt agaaaacagc aagaaacgag gaagtaatat gttccttgtt
    37741 tcgcgctgtt ttttttaatt attttttata attctggtat acttttcttt cctttgtacc
    37801 acaactcttc tggcgtatac ctgtcttttc catttatgaa tactgttcca tctgcttcaa
    37861 tcgttacttc agtcccaatc cacatattac gagaaagtgc gagaagattt tttctttttc
    37921 ctcttccttt ttttccaaat tgcaaggcat cggttatctc ttctgtccaa tgagatttca
    37981 cgtattttac ttctccgttt tcaaatgtaa ctttcaaaat attcgctata ttttccactt
    38041 ttactgcttt aggaaatgac tcctccattt ttttcacttt ccttcctcca acactaagta
    38101 tgcgctttct tttagtgata agtatatcat agtttattct attatcaaaa aaacgcatcc
    38161 attccattgt agaatacttt accgaacaag ttgaattgtt ttcctctttt tcacctttta
    38221 ctatagatag gtaaagacaa ctaaggagga atctctcatg acagatcaac cgtatgattt
    38281 aagaaaagtc atcgaagaac taaaaacact tccaggtcaa tatcacgaaa cagatattga
    38341 aatcgatcct gaagcagaat tatctggtgt ttaccgttat atcggtgctg gcggtacagt
    38401 aaaacgtccg actcaagaag gaccaactat gactttcaac aatatcaaag gtttccccgg
    38461 cacacgtgtg aacatcggaa caatggctag tcgtaaacgt gtaggtgaaa ttttgcacca
    38521 tgattatcga acacttggtc gtttcttgaa agacgctgta gaaaatccaa tcaaacctgt
    38581 gaaagtagac aaagcagacg ctccagcaca agaagtcgtt catctagcta ccgatgcaga
    38641 cttcgatatt cgcaacattt tgcctgcacc tacaaatact cctgaagatg ctggtccata
    38701 catcacaatg ggtgtagtct taggatcaga tccagaaaaa acaatgagcg atgtaacgat
    38761 ccatcgtatg gttctagaag ataaagatac gatcggtatg tacatcatgc ctggcggacg
    38821 ccacattggt cacttccaaa aacaattcga agccttgaat aaaccaatgc ctatcactat
    38881 caatattggt ttagatccag caattgccat tggcacgact ttcgaaccac ctaccactcc
    38941 acttggctac aatgaactat ggtgtgcagg agctttgcgt aacgaaccta ttcaaatcgt
    39001 tgatggtata gcagtcgatg aagtgggtat tgctcgtgct gaattcatca ttgaagcaga
    39061 aatcatgcca aatgaaacaa tgcaagaaga tatcaacacg aactcaggaa aagcaatgcc
    39121 agaatttcct ggctatactg gggatgctaa tccagctgta caagtagtga aagtcaaagc
    39181 agttactcac cgaaaagaca atccaatcat gcaaacaacg attggtccca gtgaagaaca
    39241 cgtatctatg gccggtattc ctacagaagc aagtattctt gccttggtcg ataaagctat
    39301 tccaggaaaa gtagtaaatg tctacaatcc tcctgcaggt ggtggtaaat tgatgaccat
    39361 catgcaaatc cgtaaagaaa acgaagcgga tgaaggtatt cagcgtcaag cagctattct
    39421 tgctttatca gccttcaaag aattgaagac agtcatttta gtcgatgaag atgttgatat
    39481 tttcgacatg aacgatgtag tttggacttt caacactcgt ttccaaggag ataaagatat
    39541 catcgtgatc ccaggaatga ggaaccatcc attggaccca tcagaattac cagaatataa
    39601 tcctggctct attcgtttcc gtgggataag tgcaaaaact atctttgacg gtacagttcc
    39661 tttcgatatg aaagaacatt ttgtacgtgc aaaattccaa gaagtagaag attgggaaaa
    39721 atatttgaaa taatgaatga tctgcaactg ttttgacatc ataaaaagga gtgcggaaaa
    39781 tggaacaatt ggcacaacgg aagaaaaaaa tcgtgattgg tgtaagtggc gcttcaggca
    39841 ccatttatgc catcaaccta gtgaagaaac taaaagaata tccgatgata gaaacacacg
    39901 gaatcatcag tccatgggca aaacagaact taaagctgga atccgactta tcactaactg
    39961 aattcgaaag cctgtttgat taccattatt caaacaaaga tttaggtgct gctgtcgcca
    40021 gcggatcatt tttgaccgat ggaatgatca tcgtaccggc aagtatgaaa acagtcgcag
    40081 gtatctctgt tggtttaggc gacaacctga ttgcacgtgc tgctgatgta acaatgaagg
    40141 agcaacgaaa gttagtcatt gttcctcgtg aaacgccatt aaatgccatt cacttggaaa
    40201 acttgactaa attagcacga ctaggtgtgc aaatcatacc accaattcct gctttttata
    40261 atcagcctca aacgatccaa gatcttgtta atcaccatac aatgaaactt ttggatcaat
    40321 ttgaaattgt aaatgagcat tcaaaacgtt ggcagggtga ttaatatgga aaaagaaacg
    40381 atagttgaag taaccaaact aggtttcacg atccaagctg ctgttaaagt aatcggtaaa
    40441 gacttattga tcactttaac aggcggagat acaccacata tcggaacagt cactacattt
    40501 tctcgtgata cagagatgca aactgtacgt ttccctagcc acgatggtcg gttccataaa
    40561 gacgatgtct tagccgaaaa aatagctgtg atcatcaaaa ctgccgttcc gggaagttgt
    40621 acgatcactt ccggtatcca tgttgaccat atctccaatg cacaaatcaa cgcctctttt
    40681 ccgatgtcag aagagttagg ttacaagatt ttagattggt tggaaaaaat agatttccat
    40741 atcaaagaac cagtttatta taaagatggt gaaaaaccat tatgaaagaa gaggttgtga
    40801 aatcagcgtt atgacctgag tatgagagaa aaaaatggaa aaatagcttc tcatattttg
    40861 ccgtttttga gctaataccg agggacagct gattgaacgc cgtttatcaa gaggttgtga
    40921 taaagccgct tcgtttcgag tagtaagaaa aattttgtaa acatgggttt ccacattttt
    40981 gcataaattt gacttactac cgagaaactg gcttttgaac accgtttatc aagaggttgt
    41041 gacacgtctt ttgtcacaac ctcttttatt tttgcttcac tgtaactgct acaatatcac
    41101 cgatttcttt tccgatgttt ttacgaatat cttttcgtag tccaaggatg tagcagactg
    41161 aaccatctgg atttttcacg cccatattca cgatactgcc ttcatatggc acattgtcaa
    41221 aagttgcttg gactttaact cttccagcac cgaattcttt tcttatatca tacggaaaaa
    41281 taatatatgc cccgcccttg ccgacttcgg aagcatagat ttttgattca aaagaataaa
    41341 tttccctcat tttatttctc ctatttttta catctagtat agcatagcaa aaaaaagaag
    41401 gaactgtgac aaaagtcttg tcgcagttcc ttttttcgaa taaacggtgt tctcctgcca
    41461 actcctcggt cacaagtcac aatagtcaac aaacatgaga agctgttttt tgaaattgct
    41521 ccttgtgact cagagttaaa cggcaggctc acaacctctt ccttaaacgg tgttcaaaag
    41581 cttgcatcta cggcactaag cccaatcaga tgaaaaatat ggagtgctat ttttatctga
    41641 ttgttcttac tgcttgatgc agagcagctt tttcacaacc tcctacatgg tgttcaaaat
    41701 attattcgcc ttttgcttct ttcactaatt ttgcgaatgc ttgttgttgg aattcagcag
    41761 ctaaggcttt cgtacgttgt tcttgctctg ctttgctcat ttttggattt tcagtcattg
    41821 cttttaccat accgtcttga acataagctt ttgcttcact ttccatcgta ctttgctgtt
    41881 tcttcactaa ttcagctaac ttggctgctt ggtccgtact taattctacc catgtctctg
    41941 ggattttgaa tgtattcact cggctgtcat attcgtgatt gttatcagca atattgaacc
    42001 agaatttata catgtcgttt ttgtattccc aataagtagt tttcgtcact ttttcagcat
    42061 cgccgtctgt cttttctact ttatttgttt cgttatccgt accagttgct tttggttttt
    42121 cttgtttttc atttgttcgg taaagataca ctttttcttt tccattccct aaagctttat
    42181 acaaaagcat atctgctcct tgggaatcag cactggaaac aattttttct gtttttgttt
    42241 ccgttacttt ttccattcca aagtgattgg acaaattagc gacgatcaaa cttagtgaag
    42301 cgacgaagat tagtccgaaa atcaaagata aaaaggtttg ccatgttttt ttagcgaaca
    42361 cgttcgttaa agcaaaagca agcaagctga caatcaaaac tccaataatc atgcattatc
    42421 ccccgctttc attagaacag gttctttttt cttcaataaa aaggcaataa taaagccaag
    42481 aatcccaaag atgatcgcta cgataaacgc ggcatggtaa cctgacagtg tggcattgat
    42541 tgcctgatct ttataagcta atggcgtatt atccaataca gatttgcttg ggatatttgc
    42601 ttttgtcaca ttcgtcaaca cactgatcaa aatagctgta ccaatcgagc ttgcgatttg
    42661 acggaatgta ttatttaccg ctgtcccatg actgatcaag ttatttggca atgcattcat
    42721 accagaagtc gttaccggca tcatgaccat ggagatccca aacatacgga tcgcataaag
    42781 gatcacgata tataaaactg gagtcgtttt tgtcaagaaa gcaaatggtg cagttgccgc
    42841 tgtcaaaatg aacatccctg tgatggctaa acgtttcgcg ccgtatttat cgaaaatcgc
    42901 acccgtcaca ggcatcatag cccccatgat caatgcaccc ggcaacaaca tcaagccaga
    42961 atggaatgct gattctccac gaatattttg aatatacaat ggtacgacca tttctgcccc
    43021 taccatcgcc atatttgtta caccagctaa aacagctgca atcgtgaatg tagatgattt
    43081 gaatacacga agttccaaga atggatgttc caaatgtaat tgacgccatg agaacaaggc
    43141 gatgaaaatc acaccaacaa ctaagaaacc aattactttc aagcttcccc agccgtcatc
    43201 tcctacacta gaaaatccgt aaagtaaact accgaatcca atcgttgata aaatagcgga
    43261 taaaatatca atgctaggat ttgacaatgg aataacactt ttcattagga agaatgctaa
    43321 aaccaagaca agaacaacga ttggcagtac cataccaaac aagtcacgcc aatggtagtt
    43381 atcaatgata taaccagata atgtagggcc taaagcagga gcaagtccaa taacgatccc
    43441 tgacatcccc attgctgatc ctcttttttc tggcgggaaa atagatagca tgatattttg
    43501 caataatggc atcgatactc ccacaccgca agcttggatc aaacgaccga ttagtaatgt
    43561 actaaaatta ggtgctgtaa agcaagtaat cgttccgatc aaaaagatcg tcatggcaga
    43621 aagatataaa tttcttgaac tgaatttatt gatcagccat gctgttattg ggatcatgat
    43681 cccgttgact agcaagaaac cagtcgttaa ccattgcaca tcagacgctg aaatatcgaa
    43741 agctttcatc aatgtaggaa aagctgtcgt caataaggtc tgattcaaaa cggtacaaaa
    43801 tgtaccaatt aataagacaa caactaaaag tgtccgatta tatggttttc cgtaaatatc
    43861 tacgggttgg ttgttactca tatttctcct tctttctgaa ttttttctga aactactaga
    43921 atactttagt cctaaattcg ctatttgtca acctacttga cacataagtt aatacggata
    43981 taattaaggg aagcaagagg acaaaaggag tgacaaaaat ggttggggct tcccttaaac
    44041 gtttgttgga aaatgatcaa ttggttgttg gcatcagtga attaagtgat atcgtaggtg
    44101 tgtctccacg tcaattaagg tattgggaac aaaagggatt cattcgttcg atcgaagctg
    44161 atgcgaattg tcctagaaaa tatcgtttac ctacagtcat aaaagtagaa ctgatcaaaa
    44221 aatatttaga tgacggattt actttgtcaa aagctgcaga aaaggcagaa atccgccaaa
    44281 agaaaatgca ccatgtccgt aaagtttttt caaaagcaat caaagatatc gaattgatcg
    44341 acgaacgcta tacgatttta caaatcggta acttcaaaga agagaatgct aagatgtaca
    44401 tcattcatga caacgagaca gacgaattaa cttacaagct ccttcctgtc gatgaaaaag
    44461 ctgactacga acagttgtta aaagaactct aaacgccact aatctcctaa gtaccaagct
    44521 ttttaataag tagatgctac ataaatactt gaaaaaaggc ctgtttgaac ataagaatat
    44581 ccgaacggtg atctgcgttt ctttcatata gaaaggaatt ctccatcact gttcggatat
    44641 ttttttattt ttttcggtta ctttcttcta atacatctgt tagccaattt gcatattctg
    44701 tttcccgatt gatcgcatgc gctaaaatca ggtagtgtcc gtaattttca tgtttttctt
    44761 tttcagtagg aaagatcgtt ttttttcgct ctaatagatg aagtcgtttt tcttcatgaa
    44821 ggtcacgctg ctgctgaata atttctacta atgccggatc attcacgtct ttgacgaaat
    44881 acaatttcag aacaaattcg tctcgattaa ccggtagttc attggtgggt gtatgaatcc
    44941 actctgccaa ttgttccttc ccttcttctg ttaattcata aactttcttt tccaattttt
    45001 cacctgtgat ctctacttga tgctgaatga ttccttgctc ttccattttt gcaagttcag
    45061 gataaatctg actatgtttt gcttgccaaa attctcctat ttcatttttg aacgattgat
    45121 tcaattcata tcctgtcatc ttcttcttac ttaataaccc taacaaaata tatcgaagtg
    45181 tattttttct tgccatcagt tttccctcct tgacaaatct ttaaatccat tctatcataa
    45241 atgagtaatc gaaaacatgt agtttattac atgttttaat ttataaggag atgcttaaac
    45301 atgaaagaat ttaaagaatt ggaagatttt attggaactc actttatcta tacgtatgac
    45361 aatggttggg aatatgaatg gtatgccaaa aatgaccata ctgttgatta ccgtatccat
    45421 ggcggaatgg tcaaaggccg ttgggtaaaa gaccaagaag ctgatatcgt taaattgacg
    45481 gacggcgtat tcaaaatcac ttggacagag ccaacaggga cagacgtggc attggacttt
    45541 atgccaaatg aaaagaaact tcacggaatg atctttttcc ctaaatgggt tgaagaacat
    45601 ccagaaatca ctgttacttt ccaaaacgaa catatcgcag aaatggaagc agctcgtgaa
    45661 aaatatgaaa cttatccaaa attacttgtt cctgaatttg ctacgatcac ttatatgggt
    45721 gatgcaggta tagacaatga agatgttatt agcgaagaac catacgatgg aatgccagat
    45781 gatatccgta gcggaaaata cttcgatgaa aattataaac gagtaaaata aggttgtgaa
    45841 agagaggttg tgaaatcagc gttctgacct gagtattaga gaaagaaacg gaaaaatagt
    45901 tcctcatgtt ttgccgtttt ttgagctaat accgagggtc agctgattga acaccgttta
    45961 tcaagaggtt gtgaaatcag cgttatgctt tgagtaataa gaacgctagt tagaaaatag
    46021 cttttcatat tttctaggct agtgggctta ttaccgagaa gcagccgttt gaacaccgtt
    46081 tatcaagagg ttgtgaaatc agcgttttga cctgagtatt agagaaacaa atggaaaaat
    46141 agttcctcat attttgccgt tttttgagct aataccgagg gtcagctgat tgaacaccgt
    46201 ttatcaaaga ttgtaacaac tgcatattca cacagttaaa ttagtgactt caaaatgaaa
    46261 taatgctccc agcaaaaaat taggctggga gcattatttc gttattctgt tcatcaaaaa
    46321 tcagcgtttt gattccattt catcacaagt tcctcctcat ttactgcttc atctttttgc
    46381 tgtgtttgaa ttgtaaaacc ttgtgaaaga tagaaatgaa tagctgattg atttttttga
    46441 tagactgtca aagtcagtgt tttattggtt tcttttattt ttttcattag ttgcgtaccg
    46501 attctttgtt gccggtattc ccttttcaca aagattcctg cgataaactg atcaatcacc
    46561 ccagcaaaac caataatttc ttgatctatt tctgctaagt aaatcactgc atctggtaaa
    46621 gctttcctaa cagattctac ttgattctcc caataagttg atggaatgaa ttcatgagaa
    46681 tccaagttag catgcagcca aatcgataga acagattcca gttctttggc agacaatttt
    46741 gttgttgtgc ggatcatttt ttctctcctt acttattcat tgattttaaa ataaaaacca
    46801 tttttttgaa gctcttttac aagttttcgc ccctcaaaaa agtatatctt ctcttgtttt
    46861 acttttctaa taccaaaaga aacagcaact ttaggatcat cagctgaaac gattcgataa
    46921 gttccaccag gcaattgatc cagcttgact ttcccatgat ggtcgctttg tactgaaaac
    46981 ggcacaccat ttctagtaag tgtttttttc ttatgtttca aaatgaatga actaccattt
    47041 accggtttcc ctgacgaatc actaataata aatgaaaaat caagcacttt tttacttaat
    47101 ctatactgat agattagtcg tccgataaac actaacagta tgactacagc tattatcgtt
    47161 cctgcaagaa taaaccattg ccgcatggtt ctttcctgat ttccttttcc ttcttctttt
    47221 tttaccgatt cgttataagg aatccgatgt cctgtcacta gcaaacgatg cgtgttgatc
    47281 atatacggcg tacatgtaag caaggtaact aagtcttctc cttctgaaat cgttagaaca
    47341 gacgtgtcat ctggcagtac cacttgtata tttttaactt gataagccaa tttctccccc
    47401 aaaacagtca aaataaacgt gtctccgttt tctaaacgat ctaattcggt aaataaccgt
    47461 cgattaggta aaccgctgtg agcagagatc actgaatgcg tcgaatttcc accaagcggg
    47521 aaagaggttc cttgcaagac gcctgctcca ttttcaagga tcctaccatt gagccgatca
    47581 taaagtgaaa tatttaattg gatcttaggt atattgatac ttccgatcag ccattcctct
    47641 aattggctgg atgttgtact ttcatttctg ttcttttcat tgaaaggatc agtaccagga
    47701 ttgaatcctt ggtcagttaa tcgttggttt ttctcctcca ttttctttcg ctgcacttca
    47761 atttcagctg cggtccgttt ttgtacttgt tcgatacgtt tttgatcaat aaaactattt
    47821 aaagaatcca cataaaatgg atataaagcg atcagtaacc ctacgacgat acatattaat
    47881 aaaaacaaac gaaacaaaag tcgacgagac tgtttttgct tactctgctt cacatgtgtc
    47941 ctcccttgca agtttttttg ttctttcttt tattatatgg gttcaggaaa aaaatagaaa
    48001 atgaaagccg atgaggttgt gaaaaagctg tcctgcatca agtaataagt acagcccaaa
    48061 aaaatagcta ctcatatttt ttgttgggtt gggcttatta ccgcagatgc aagcttttga
    48121 acaccgttta aggaagaggt tgtgaaaact gcgttatgct ttgagtaata agagcgctag
    48181 tcagaaaata gcttctcata ttttctaggc tagtgagctt attaccgaga agcagctgat
    48241 tgaacaccgt ttaccgagag gtcatgaaaa agctactttg tttcgagtat taagaaaaat
    48301 tctgtttcaa aacaaggaga tgcaacaaaa cttatgtcgc atcctctctc cttagaactt
    48361 tcacttatgt ttttcttgta aaattcataa atattttcat gaattttcta atttcttgcc
    48421 tacctctcgc ataggatata tgatgaattt atctaaagtt tgcaaaggag ttcgacaatt
    48481 gaaaaaatat ggcccatttt tagcaatttt tttcattgct ttggcatttc tcatgcctca
    48541 tttattcaca cataaactaa ttttgggtgc tgatgcccca ttccattaca atcggttcta
    48601 tgatgccgcg caacaaatta aatatgggga tttccattat tttccttccc tttatggatt
    48661 ccagcaaagt ggcaggatcg tgaatgccgt ttacggccca tttttcgctt atttacaagg
    48721 attgatcgtg ttgcttgcag gtagctggtt tcattatcag attctttcaa atttcattct
    48781 ttatcttatt tcaggatttt ctatgctgat tttactgaga tatgtcaatg ttcgaaattg
    48841 ggtaagtgta ccagtagccg ttttattcat gagtacgtat tctattcaat actggtctat
    48901 caaccaagga ttcactagct gggggacttg tttttatcct ttatgtatga tccctattgc
    48961 tgatttcgtc cttcataaaa agttccctgt tatcaaagtc gctgtctcag tgggacttat
    49021 gacacagatc catttactgt cttcgatcat gttagctttg atgtatatcc ctttttatct
    49081 ttattacttt tttaaccaga ctgaaaaagg aaaagcactg cttcaactga ttaagtcggt
    49141 cttgctttat tttgttctga cagcaaatat ttgggcagga ttagctattg tttacagtgg
    49201 gaataagatt cttcctccct ttgttaacag agaaatgagt caaaaggcga tagatctgga
    49261 tggcagttat tggctccatt accctaaggc ctttcctata ttgatattat caggtttttt
    49321 gctttttatt ttctttcaac aaaagaacac cttattccaa aaaatcgtgt tgtacacaag
    49381 tcttgttttc ttttttcttt ctactagctt gattccatgg acgacattga tcgaaaaaaa
    49441 tgtgcctttt attagtttga tccagtttcc atttcgattt ttcgtgccat ttacgctatt
    49501 gcttctactt taccttgccc tttcactgaa ccagtggtct gaacaaaagt ggttccgtct
    49561 tttaagtata tttggcattc ttattttttc agcacagacg atccagaact tataccagca
    49621 tctggaaaag tgggaaaatg aaacatttgt cagtcgacat acttatctat tcgatacgcc
    49681 ggaagaagca aggaaaacat tcttcagcaa agatatgtct accagcttgt tcgttttcca
    49741 aaaaaccaca cctgactatc tgccaatcta taaggagaca aaagacaata aatacgaccg
    49801 gtataacgaa gaaatactag aaaacgaaac aacttttata aaaacacatc caggcggtct
    49861 tttgacaatc aagtggaaaa acactgggga caaaactgtt catatccctg ttatcgtcta
    49921 cgatcggact gtgctacagc aaaatggaaa aacattgact gattatgaag taacagatat
    49981 cgggacccca atcgtgaagc agcaaaaagg aatcaacgag ctcacgcttc attatcaaac
    50041 gccaatctat ttctatttca ttctttctct tactttgatt ggctggttta ccttgctatg
    50101 tttatttatt tatcatagat acaagctttt acgtgcttag gcaaaaggga taaatacatc
    50161 cgatcaaaaa actctccaaa cggtataatc gtttggagag ttttatttta atcagttttt
    50221 ttatgttata tgttatattt tttattcgtt cacatctaat tcgatttcca ccccgtagtg
    50281 atcactgatg attggactat cgatcccatc aaaaatccgt tgatattttt taacctgcat
    50341 tccttcaggg acgaaaataa aatctatacg caatttttct gtattttgtt cccaaccatc
    50401 tattttcttt tccacagtag ctgcttttcc tttttcattc gcgacaacat acgaatcttg
    50461 aagaagaagg caactttctc ttacagtata ataaccttga gagtctactg gattattgaa
    50521 atcacctagg aaaaaaagtg gtacgcgagt ttccaaaaag tagttttcca agtttttcca
    50581 ctcaaaataa aagccacttt ttttatctgt ccaccaagaa aagtggcagc tcgagacaaa
    50641 aatgtcctga tcatctatct ttgttttccc aatcaagatc ttgcgtgtct gataatcttc
    50701 tttcttttga aattgggaga cggtaaggac ctgactttcc aatgtttttt tcgtaagcag
    50761 tgcatttcct tcttcatatt tatcataacc gatgtggctc atttcccaac tccaaaaata
    50821 ctcttttccg tgttccttta aaagctgaac taaacggtag gcaaaattat ctttacggat
    50881 aggtacttga ttctttaccg gacagaactt cattagcttc gcaggttcta cttcttcaga
    50941 ttccagtaat tggttgattt cttgtaaagc aatcacttca taatcttcta acaggatctt
    51001 atctgctaat tcttgcaatt ttttctctgg ttctggctca agccagctat gcgtgttgag
    51061 tgtcatgaat ttcattggtt ttctccttgt cttcatttcg aaacaaaagg gttgtgacaa
    51121 aatttttgtc acaacctctc cagcaaatag tgttctcctg ctaactcctc agtaataagc
    51181 tcaatggacc aggagctatt ttttgtttgg ctgtttttat cacttggtgc agggcagctt
    51241 tttcacgacc tctccactaa acagtattca aactgctgct tctcggtaat aagcccaacc
    51301 aaacgaaaaa taggaggagc tatttttgtt tggctgttct tatgacttga tgcaggacag
    51361 ctttttcaca acctctcctt acattttaat ggagccgatc actttaccag ctgtcactgt
    51421 acctaattgc ttgatcacta cttcttctac tttatcgttt gtaaatacga tcatgatcgt
    51481 tggatctttt tcttcgtttt ggatcacatc aagatccatc tcagctaatt tcgttcctgt
    51541 tcctacttct tggccttctt tgacagcaat ctcaaaagct ggctgattca tttggactgt
    51601 atcgattccc atatggatca aaacttcgat accatctttc gtttccagcc caattgcatg
    51661 tttgctaggg aaaatacttg tcacttttcc agcgatcggc gcaacaactt ctcttgatgt
    51721 tggaataaca gcaaagccgt cacccatcat tttttgtgag aataccggat cttttacttc
    51781 agataaagga atcacttctc cagaagctgc ggaaactagt tcttgttcta tacctaagta
    51841 ttgcttacta cctgcttgtc catttcctgt agaaaccatt tctggctctg gaatctctgc
    51901 tcctgattgc agcagatctt cgatatctga tttcaagaca tctgcttttg gtccataaac
    51961 tgcttggact ccattgtctt tgacgatcaa tcccatcgcc cctgctcgtt tccaggcttc
    52021 ttcagtccct actttttctc gatcatttac acttacgcgt aaccgagtca tacaagcatc
    52081 gacatctttg atattttctt ttcctcccag caaatgaatg atctggacca cttgctgatc
    52141 tgctgttcct gttccgctgt tagatacaac ggtttcttca ctgtcattgt cgtaatttcc
    52201 gttacgtccc ggtgtagcaa agttgaattt tttgatcaag aaatttgcta agaaatacgt
    52261 aacaacacca aaagcgatca cacacaacac gaagttgata agatctccac ctagacccgc
    52321 tttgatagct aacggtgtcc gagtcagcaa ttcgatattc ccaaatgaat ggacccgcaa
    52381 tggcaagata tctgccatcg caaatgcagc cccttggatc actgaataaa tgacatataa
    52441 tggcacagca gcaaacataa acatgaactc caatggttct gttacacctg ttagaaagac
    52501 agccaacgct gctgaaaaat acattgattt atatcttgct ttcttatcag gatcgacatt
    52561 acgatacatc gcaaaagcca ttcccatcaa gatacctgaa gaaccaatca tttgtcctac
    52621 tttgaaacga gccggcgtcc agttatgcaa cacaaactca tattgcgaca tatcccctgc
    52681 acctttaaga ttcactaaat cagtggccca agccaaccat aacggatctt gcccaaatac
    52741 ttgtgtcccc gcctgtgcgc ctgataaaat ctcatacgat ccgccaagct gtgtatagtt
    52801 gataggaatc gtcagcatat gatgcaatcc aaatggcaat agtaaacgtt ctaatgttcc
    52861 aaataaaaac ggtgctagaa tcggtgcgct ttcttgtgat tctgcaatcc ataaaccaaa
    52921 attattgatt ccagcctgta cgtttggcca caccacagca agaatcaatg ccacgattgt
    52981 tgaccataag ataacaacaa acggaacaaa gcgctttcca ttgaaaaatg acaaagcatc
    53041 cggcaatttt ctataattat agtacttatt ataagccatt gccccgacaa atccagcgat
    53101 gatccctacg aatactccca tattcaaagc tggtgcttcc aatacacttg taaaaaatcc
    53161 tttaaccatg atcttcgtac cgaacagagt gtgggtaaat gcatcttcat tcgcaagcat
    53221 gtcacttgtg acaccaaaga tagccccagt gatccgattg attaaaataa atgagatccc
    53281 agctgcaaat gctccgcctg ctctttcttt tgcccaactt cctcctattg ctaaagcaaa
    53341 taaaatatga agattaccaa taattgccca accaatattt tcaattactc cgcccgttgt
    53401 gacaagcggc gtccaatcag gattgatcaa gggaattgat ttccctatac tgatcatcaa
    53461 cccagctgcc ggcataactg ctaccacaac cattaaagcc tttccaaatt tttgccaaaa
    53521 ctcaaaacta aatatctttt tcattccgtt tagctccttt acgcaatcgg tttcgttaaa
    53581 cgtattatag cgtgcgtttt cttttttgta aatgctttat tttttatttt tctgtctata
    53641 ccattctata aaacctataa tacgtgtgtt cccttctata attttctcct aaaaatttat
    53701 ttttttaagc ttttttctat gaaatatttt cttttgtcta ttgacttgtg caatcggttg
    53761 cgtttattat gaacgtataa aacagctcat taggaggaga aaaatgaaac agatcaaacg
    53821 actatttgct attgatccat ggaaaatcag cacgacacaa ttagataaaa aaaatcttcg
    53881 actgcaagaa tctttaacaa gtattggaaa cggttatatg gggatgcgcg ggaactttga
    53941 agaaacttac tcaggggacc accatcaagg tacttatttg gcgggtgtct ggtatcccga
    54001 taagacacgt gtcggctggt ggaaaaatgg ttatccagaa tactttggaa aagtgatcaa
    54061 cgccttgaac tttatcgcga tagatatttc gatcaacgat cagctgatcg acttggcttc
    54121 tttggaacca gaagactttt actgggaatt agatatgaaa aatggtgtat tatctcgtac
    54181 attcacaatc actacttcta ctaataaagt acgcatttcc ttcgaacgtt ttctgagcat
    54241 tgtcaaaaaa gaatcagctt atatctattt aactatagaa atgcttgaag gtagcggaac
    54301 agtcaaactc gtatcaaaat tggatggaaa tgttcaaaac gaagacagca actatgatga
    54361 acatttttgg aatgagattg atcgtggaac tatagaagaa atcggctatt tgacgactaa
    54421 aactattcca aacaactttg ggattgaaca atttactgtt actgcagcaa tgcgccattt
    54481 cgttaatgga aaatcagtca tccctgacta tcacgaagat actttagcat tacatggttg
    54541 cttgtctgca gaattatctc aaggtgagac atggtcagtg gaaaaagaaa ttatcgttct
    54601 tactagtaga gatattccag aagcgcagca agcagaaaaa gctgtctcag aattaacgaa
    54661 ccagacaaac aattatatcc aagcaaaaga agagcaaaca gcggcttggg cagaacgatg
    54721 gaagcttgcc gatgtggtca tcgaaggaga cgaagaagca caacaaggta ttcgattcaa
    54781 tttgttccaa ctattctcta cttactacgg cgaagatgaa cgattgaata tcggccccaa
    54841 aggattcaca ggagaaaaat acggcggagc cacttactgg gatacagaag cgtatgctgt
    54901 ccctctttat cttgctttag ccaaacctga agtaactaag aatctgctga aatatcggca
    54961 taatcaactg ccgcaagcaa tccacaacgc tcagcaacaa ggattaaaag gcgcgcttta
    55021 tcctatggtc accttcactg gtgtcgaatg ccataacgag tgggaaatca cattcgagga
    55081 gattcaccga aatggcgcga ttgcctatgc aatctacaat tatacgaatt acaccggcga
    55141 tacggaatat ttgaaaaatg aaggactgga agttttagca gaaatcgcac gcttctgggc
    55201 agaccgtgtc catttttcaa aacgagcaaa taaatacatg atccacggtg tgaccggccc
    55261 aaacgaatat gagaacaata tcaataacaa ttggtataca aatacgatcg cagcttgggt
    55321 cttgcgttac acgcgagaaa attatttaac ttatcaaaaa gaagctgcta tcactatcag
    55381 cgaagaagaa ctagccaaat ggaaagatat cgcagagaac atgtacttcc cagttgacga
    55441 agaattagga atctttgtcc agcatgacac attcttagat aaagatctca tgcctgtatc
    55501 cgatattccc acaagcgatc ttcctctgaa tcagcattgg tcttgggaca aaatccttag
    55561 atcttgcttc atcaagcagg cagatgtact acaggggatt tatttcttca atgatacttt
    55621 tacaaaagaa gaaaagcaaa aaaactttga tttctatgaa ccaatgactg tccatgagtc
    55681 ttccctttcg ccaagtatcc attcgatttt agcagcagaa ttaggaatgg aaagcaaagc
    55741 tgtagaaatg taccagcgaa cagctcgctt ggatttggat aattacaata acgacacaga
    55801 agacggcttg cacatcactt ccatgactgg tagctggttg tcgattgtcc aaggatttgc
    55861 tcaaatgaaa actgcccatg atcagttgag attcgcacca tttctgccaa aaacttggaa
    55921 agcttattct ttccatatca attatcgcgg acgtttattg ttcatctcag tcacagatga
    55981 acaagttact ttcgagttat tagaaggttc tcctctagaa atttacgtct atgaacagtc
    56041 ttattcttta gcggatcgtc tagaaataac gctaagaaag gaggaagcaa atgtttaaag
    56101 gggtattgtt tgatttagac ggtgtcatta ccgatactgc tgaattccat tatcgtgctt
    56161 ggcgaaagct tggtcaggaa atcggcatta cgatcgacca cacattcaac gaacagctaa
    56221 aaggcgtcag ccgagaagac tcactctctt tgcttttagc ttatggcgga aaagaacact
    56281 ctttttcaaa ggaagaattt gcagaattag cgaaaagaaa aaacgattat tatttagaga
    56341 tgatccagac gatcgaacca aaagatgtct tccccggggt cgtgccttta ctggattctt
    56401 taaaagaaga aaaaatcaag attgctcttg cttctgcaag taaaaacggg ccattcctac
    56461 ttgaaaaaat gggtcttaca ccttactttg atgctattgc cgacccggca gaagcaacaa
    56521 acggaaaacc tgcgccagat atcttcctcc ttgcagcaaa agctgtcgga ctaaaagcag
    56581 aagaatgtat cgggatcgaa gacgcgcaag ccggtatcca agccatcctc tcaagtggcg
    56641 ctcaaccaat tggcgtggga cgtgctgaag accttggaga ggagatcccg cttgtccctg
    56701 atacgacttt tcttacgatc gattacttga aagaaaagtg gcatgaccat ggataatcca
    56761 gagatctccg tcgtaccagt tccagagact gaaataaaga aaatcacatt gaagaacgac
    56821 tacttagaag tcgttcttct caattacggt gcccgcctgc accaaatctt cgcaccagac
    56881 aaagaaggaa aatcagaaaa tgttttattg tcttacgatc gtttcgaaga tgttctaaca
    56941 gacaaaagct tttttggagc aactgtcgga cctgtagccg gaaggatccg ggatgcttct
    57001 tggggtgagc atagattgga gaaaaactgc ggatcacacc atatccacgg tggcacgaaa
    57061 ggctggtctt tccaattttg ggaagtagaa gttttcaaaa ctccatcatc aataggtgtg
    57121 gtcttttact taaaagatga attttctacc tatccaggac caatcactgc tacgatcact
    57181 taccgtttaa caaaaaacga attagagatg acaacctctg ctagtagcct tgttgaaacc
    57241 atctgcaatc cgaccaacca tgcctacttc aacctttcag gaaatggaac aagagacatc
    57301 tatgaacatc agttaacggt ctttttagat ggtgtgcttg aattagatca agaaaaactg
    57361 cctactggaa attggacaaa aaaagaaaaa ctgccaatcg actttagaaa atccccaaca
    57421 ctgcaagaga tccttgcatg ttatccagat ggactagatg atgtatttct tttgcatcat
    57481 cctaggcttt ccaaaacttc cttacaatta ttcgagaagc attctggtcg ccaaatgact
    57541 atagcaactt ctaataaaag tatggtatta ttttctacga ctggattcga agctgatttc
    57601 tctgtcaatg gaaaacagat gcattcaaac tacggattag caatcgaacc gcaagaaatc
    57661 cctgatatcg ttcatttccc caaatttgga tcgatcaacc tccatcctgg tcaagaacgg
    57721 atcagtcaga cgatttatcg gttctctgca caataacaag agaaaggtgt ttttatcatg
    57781 gctattaccg taaaagatgt ggcgaaaaaa gccggtgttg ccacctcgac cgtctctcgt
    57841 gtcatcaacg atcatcctag tatttccgag acaacaaaga agaaagtccg aaaaattatg
    57901 gatgaactag gttatgttcc taacatgact gcccgcaatc taggaaaacg catttccagt
    57961 gccatcggcg taatcttacc ccctcttgat tcaaaagagc ggatcggtaa cccattttat
    58021 cttgagatca tggaagcaat caacgaagaa gcgcggaatt atgatatgac tacagcaatc
    58081 gctactgcta aaaattttga attgctgtta gagaacgtcc agcgtatgca tttacaaaaa
    58141 caagtagatg gttttattct agtgtattca gatagtcaag atactgtcat tgattattta
    58201 taccaaaacc acatcccttt cactttgatc ggacagcctt atcatcatga gaacgaaatc
    58261 gtttatgtag ataatgataa ccagcttcta ggtaaacaag caacagaatt tttgatccag
    58321 aatggacata agaatatcct gtttgtgaca aacacgactc atgaaagttt gtatttcgaa
    58381 cgatatttcg gttatcaaaa agcaatgatg atggctggtc ttcctgcgtt atccagtgta
    58441 gtactagagc atccggagga ttatctgtcc tttgagaaaa cgttgaaaga aaacgaagcc
    58501 acagcattgg tggtcattga cgatatcttc gcccttcgca cgatgcagtt ggcgcaaatg
    58561 tatggctatg tcattccaga gacactctcc gtcattagct tcaacaactc gatcttctca
    58621 acattaacac acccttacct tacaagtatc gatatcgata tctccgaatt aggacgtgtc
    58681 gctatgcaaa agctgcgcga attgatccat gaagaattga gcagtggcgt acgtttggtc
    58741 atccctcatc gcttgatcaa aagagagaca gtcgttcctt tagaaaaccc atactaaata
    58801 gctgactcac taaatacgct gaccatttat cctatggtca gctttttatg taaaagaaga
    58861 tactacttct aacacattgt ttatctgtaa aaaagagcgt gggacaaaag taaagaacac
    58921 ttttgtctca cgctctaaac acgtataaac ggcggaagca gaagcaactc cttcggaaat
    58981 aagctgaaat tcacaaaaat ttaaaaagca attttcttga gtttcttctt atttcttgga
    59041 gttaaacgct tctgtcccag cctctttttt ccttccttca attttttaca cgtcgacgat
    59101 tcaataaaaa accgcctaat attgtaaaga caggtgcaag aatcgagaaa aaggcgaatg
    59161 gcaagtaatg caacgcgcta actttcatcg ttcccataat gaatgtgccg ctgactcccc
    59221 acggtacgag agaattgacg gtcgcacctg aatccgctag tgttcgtgtc aaataggttt
    59281 ttgataagcc aacgtcatca aaagaacgct tgaatgtttc tcctggaaga atgatggaca
    59341 gatactgttc tccaacgatc agattgattc cgatacagct caaggcagta aacccgacta
    59401 aacgagaagg tcggtccatc ttttctttta attcttgaac gatggtatca acgatcagat
    59461 atttgatcag taacccacct aatgccaatg caagtaaaat caaactagca gaacctaaca
    59521 tactcatgat cccgcctcga gaaagcagtt catctagctt tggatcaggt gtatttgcga
    59581 cataaccatt catcaagatg tctgctgctt ttccgaccgt caaacttttc tcatggataa
    59641 aagaaagaac gagagcagct gtcgatcccg ctagtaatgt agggatagcc ggaatttttt
    59701 tgattgcccc cccaaataaa ataactagtg gaatcaatgt ccaaggagag atccaaaagc
    59761 tcgctttcat gttttggatc atttcattga tactagctag atcggctgaa tgactgttat
    59821 gtcctaatat cgtataaaaa atcaacgaaa ggacgaatgc tgggaaatcc gtataacgca
    59881 taccagcgat atgttcgaat agattaactc ctccaatacc cgctgccaag ttggtcgtat
    59941 ctgataaagg cgagcagtta ttccctaaaa aggcaccgga aacgactgcg cctgtagtca
    60001 ttgcatcatt gaatccaagt aaatgaccga tccccaaaaa agcaattcca atcgtcgaaa
    60061 tcgttgtaaa tgaactgccg acagtcactc cgacaattgc acaaacaaga aatgctgtcg
    60121 gtaaaaagaa acgcacagaa acaagtttaa atccataaac cataatcgta gggatggttc
    60181 cagataaaat ccatgaagca actaagacac caatcaaaag aaaaatcagg attggaatga
    60241 tccctggagt gattcctttg atgatcccgt catggatctc atcccatgtg aactttttaa
    60301 atctgccata aaacaacaaa cacatgaaag ctgcaaggat cgggatatgt ggcgagagct
    60361 ggaatccgat gatcagtact cctaggagtg ccaacaacac gatcaataca gcgatacttt
    60421 cttttaaaga aatattttta ttcatcgtcg atctcctatt tcaaatgaaa aggccctgcc
    60481 atgaaaaaac tattcacttg gcaggacctc ctactaaatc agcgacaaaa cgtcggctga
    60541 aagacttatt tttttatttt tcttctaaac ttttttctac ctcaatcccc caagaatctt
    60601 tcttgaagtg attgatgatc aatggaataa cgaacatgac cactgtgata gctgctacta
    60661 ataatgcatc actcaatgtc gctgtaccta atgtagcaaa tacgacaacg atgatagaga
    60721 agatcagcat gcagctgact acccaagcta atccgtttcc gtttttacca attcggtaag
    60781 gacgttcgaa ttctggatgt ttcttacgca ttttaagcaa tgaaacagca atcaaaatgt
    60841 acacgataca ataaataaca gttgtagcat ttgtaagtgt caagaataca gcacttacgt
    60901 catccattac accataaagc aaagcaaata atgaaataac gattgattgt gttagaacga
    60961 catttctaga tacgccgtat ttgttttctt tatggaatcc gaattttggt ggaaggaatc
    61021 cttcacgagc tacttggatg attgtttttg atggtccagt aacccaagct gataattgta
    61081 aaagcacacc aataaatacc atgaagctga agatatttcc gatcacttct ggtaagccta
    61141 ggatttgaca gtcaatcaag ataggctgcg tgatatttgc taattccatt tttccatctg
    61201 gtacgacatt tgctacaagc atcgcattga tgacattcaa taaaactaat ccgatcaaag
    61261 cgataaatac gccttttgta taattttttg ttgcgtcttt caaacgaggc atgtaaacag
    61321 aactcatttc gatcccaaca aaaataaatg tgataccagc taagtatttc aatgaatcaa
    61381 tatgttccaa gtcagggaaa gcttttgacc atgaaaagtc acctagatag ccgtttgaga
    61441 ctagtccgac tttgaaggct gccaaaacac ctaaaacgaa catgatgact actgggatat
    61501 atacccctag ccatacaccg atgttcccgc cgacttttgc catatcgaat ttcaagttca
    61561 agatcgtgat gatccagtag ataactaaaa tacaacctaa aacgaaccag tgattattgc
    61621 caagttctac attaccaaat gtgtttccta acaatggtcc taaagtagaa gcgaccatta
    61681 ccatacctgg gaacatttgc acccataata gccatgcaac gacaaatccc catttgctgc
    61741 ccagtgctgt ttttacccat aactgaggac cgccttcttg aggaagcatc gtggaaagct
    61801 caccggaaac cattgatatc ggaccggcaa aaaacaaaac tgcaaaaatt gaataaaaaa
    61861 tcaatgtcca accgactgcg gctaacgtag gaatactccg aaccgttgca cacagtgcca
    61921 ttgtcagacc gataaaggtc ccaagaccca ttttacttac ttcagattta ctcaccgttg
    61981 acactctcct tgattttttg gataaaataa cggtgtcttt gaagtagttt cagcaataaa
    62041 cgaaacaatt cagcgaatat ttgaggggat tctttgagtt tattgcttga agggtttctt
    62101 caaagacaac ctcaaaacaa agggatcgaa gagcttaggt ctccccttgc tcttcagatc
    62161 caaacatgcg tcagaaacag ttagtattca agctgttttc taacatgatt accttgctta
    62221 ttttgcttcg cttgccaata attggtctgc gtagattttt tccaattttt cttgaagagc
    62281 tgctttgatt ttttcagcat attcttcaaa gtgttcttct ttgttcatgt atggtgtcat
    62341 tacgctggca cgcaatacag ttacttttcc ggcacggttc cattcttcat ctgagaatcc
    62401 tagttggttc acaaattgta atgggctgtt gccatagtct gggatagcaa agtcagtatg
    62461 tgaagttaag aactcattac cgtaaatgct tcctttcacg tatgaagaat aatcatatac
    62521 gtcatggttc aatttattca ttgcaactaa gtcatcgttg cctttttctt tgaatacata
    62581 gtctaccata ttgaaatctg gataagttaa tggatgaact tcgatttctt tgtctcctac
    62641 tttaaatgac aagtcattca agaagttgta gaaacgatgt gcaccttcga ttgatgcacc
    62701 cattaattta ccatatcctg tgacattcaa aggtaagaca tggtgtgctg cccaaacgct
    62761 ggctgctgtt gcccctgctt ttgaaccttc caagatataa gccccaagta atgctgggat
    62821 atctgcgcct ttttcaaata catatgtcgc aaagtaagag ataacgtctc tcatgcggat
    62881 atctttgatg acgataccac cggcagaata tggaacatag cccattttat gtgggtcaat
    62941 cgttacagat tctgcttctt cgattgcttt atatgcactg tgtacttctt ctaagatata
    63001 atctttgttt tctgtaaaga cattgtattt gtaatgaaca tcttttaaat cttcaaatgg
    63061 aatgaagttg ttgtcttcgt ctaagaaaat cgcacggccg tatccgccat aagcagcatc
    63121 tacgtgtaag tagaagtaaa taccgtcttt ttctaatacg cgacgcaatg caacgatttt
    63181 atcgataccg tcgattgctc cttcttctgt tgatcctaca acaccgacta cacctaggat
    63241 tggtgttttt tcagctgcta gaccacgaac gattttttca agttcgttga tgtccatgcg
    63301 atagttgtgg tcaactggca ctggaatcac ttggtctaaa ccgataccga tgatgtcggc
    63361 agcttttagc catgaatagt gtttagtttg tggaaccaac cattttccta atttttctaa
    63421 atgttttccg ctacgtgctg agtgagcttt gatttcgtcg attttttctg ggacgctgtc
    63481 taacagattc atgatttctt ctgttgacaa gttcattaat tcccaatcgc tttttccagc
    63541 aactagttca ggtgtcactt ctttcattgc aagcggcaat gatttgatgt tgcgggcata
    63601 ccaaagtcct tctaagtttg ctaaagaacc atcagcaacg atgtggcccc agccatcttt
    63661 atagctcatt aattttgcaa attccattcc tacttcttct tccatttggc ttgttgcagg
    63721 agaagattca taggcaacgt tattaccatt ccatagcata gcaaaattgt acgctagtaa
    63781 agatggcatc aatgtttcag agttcatatg accccagtaa cgtccggcat tatgccaagg
    63841 aacggaatgg gtacgcatcc gcgctgaaat ttctgataga acatcctttg ttttattcac
    63901 agtgtgttca aaactagcac tgcttttttc ttctggcgta ataataggca tgtcttgagg
    63961 catgtagttt tgacgccagc ctaaatgttc atccaccaat tcattcaaca aagctttata
    64021 gatttggccg ttctcagctt tgtccccaat gaatagggca tttaggttta gatctttcga
    64081 caatgattca ctcatagtga aaccctccta aaaaaatatt taaattttgt cgagccctac
    64141 attacacctg ttcttctatt ctgtaaacta agttttggcc tgatttttaa tcatatttta
    64201 atatgtatca attatccgaa ttgttcacac ttttgaaata attaaaaaaa acgagggaat
    64261 ttacaaatgt aaactccctc gcaaagattg tattaacgac aaccagcgtt ctattttagg
    64321 atgggtttca aaattggaat gttgtaatag tatttccctt tttcaaggta aaaacttact
    64381 aaattctaat caattacttt ggccaaaaag taatttttct taccttttcg aataaccaca
    64441 aacttaccat cgaattcatc ggatggattt atgacaaaat ttaaatcggt aacgcggtca
    64501 ccgttaatac ttatagctcc gtttgaaaca tcttcacgtg cttgacgttt agatggttca
    64561 atttttgtgg aaacaagcag ttccacgata ttttcaggtg tgcttgaaat ttcaacgttc
    64621 ggcattttac cgaatccttg cgcgatttct tccgcattta gttctttaat atttccagag
    64681 aacaatgctt gcgttatttt ttgcgcttct ttcaagtctt cttcactatg cacaaatctt
    64741 gtaacttctt ctgccaaacg gcgttgtgct tcacgtttct caggttctgt ctctactttt
    64801 ttcgctaagt cttcgatttc ttcttgagac aagaaagtaa agaatttcaa gtatttcact
    64861 acatcgcgat catcttgatt caaccagaat tggtagaact caaatggtga agttttcttc
    64921 ggatcaagcc aaacggcacc gcctgcagtt tttccgaatt tcgttccgtc agccttcagc
    64981 atcaatggga tcgttaaacc gaacgcttct gcttctggtc cttctaattt tcggattagg
    65041 tctaatccag ctgtgatatt tccccattgg tctgctccac cgatttgcaa ttgcacatcg
    65101 tatgttttat gcagatgcaa aaagtcgata gattgcagga tttggtaagt aaactctgta
    65161 aatgagattc ctacttccaa acggctggca acgatatctt ttgctaacat cgtgttgatg
    65221 ttgaagtttt taccgtagtc tctcaaaaat tcaagtaaag agatttttga caaccagtcg
    65281 tagttgttca caaatgtgat atttgcatct ttaccaaaca attttctcat ttgattggaa
    65341 agtgcgtcca cgttatgttg cacttgttcc atcgtttgca acacacgttc tgttttgcgg
    65401 ccgcttgggt cgccgatcgt tcctgttccg ccgccaatca gaatatatgg atgatggcct
    65461 gccaattgga atcttttcat catcataaat ggaatcaaat gtccgatatg catcgaatca
    65521 ccagttggat caacgccgca ataaagtgaa atgctctttt cttcaacgag ctcgcgaagt
    65581 ctttcttcgt ttgtttgctg attaatggcg tcgcgccaag ttaattcatc aataatattc
    65641 attttactga cctccgtgta gtttttttat gaaagcagga acctgctagc atgtgtaatt
    65701 aattatttct tcaggatagg ataggaaata aaaaaagtcc cttggcatat aagccaaggg
    65761 acgaaatctc gcggtaccac ccaatttgta tgtctagata tccaacatac ctctcgaaca
    65821 tttaacgctg tcgcgtggca atcactgcca aagactcctg aaatgtaatt cgctttaaag
    65881 catgtactgg tttgcagcga ccaccagctt tctgaaacat tggctttaac aactacttcg
    65941 tttcgatctt cgtcttgtta ttattaatat gtgtctatca tagagcgcaa ctttttcttt
    66001 gtcaacagaa aaaggatatt ttttttaaat tcctcatttt ttcttttttg aatccgctct
    66061 aattccgaac attttatttt caattattgt taaaagaaat aactttttct gtattttttc
    66121 tgcaatttat cgagaagtca ctttactttg cctcttattc tttcttcgtt acaataagga
    66181 atgggacaag taaaaaaatg aaaaagcaag ggacgaggta tatggaaaaa aagcaaaaaa
    66241 ttattttagg agccacttct gctttaacta tcgttatttt aattctaagt ttgttttttg
    66301 tcatccgatc atctacaaaa aaagaaccaa tcattcctga aaaaaaaata acagaaaaag
    66361 caaaagaacc gacagatgat aagcaacaac tagttactca agcaaaaaat ctggctcaaa
    66421 gctacgatta tgaccaagcg attgcgttat tggaaaaaga tagtaagaac ggatctactg
    66481 aaacacaaca gcttcttcaa acactaaaaa aggagaaaga acaactgatc gactggtctg
    66541 atcctactca gatctctcat gtctttttcc acagcttgat cgtggacccg caaaaagcct
    66601 tccactctca acaggcacaa ggatacaaag attatatggt gactgttgaa gaattcaacc
    66661 gttcgatcga acaactttat caaaatgatt ttgttttagt taatttgaat gatttgatcc
    66721 aaaaagacga acaaggaaat ctttccttta acgggcttgc gctacctgaa ggaaaaaaac
    66781 cattggttct ttcacaagat gatgtcagct attatgaata tatggataat tccggttttc
    66841 ctagccgatt ggtcgtatca aataacaatg aaatcaaaaa tgtctataaa gagaaagata
    66901 aagaagaaat cggtgacttt gacatggttc ctctgatcga tgcctttgtc aagaaacatc
    66961 ctgatttttc ttatcgaggc gcaaaaggta ccttggccct gacaggttac aacggagttt
    67021 taggttatcg aacatcaaaa tctcaatatg gagacaatga aaaaacaaaa aaagagatta
    67081 aagaagctaa aaaagtggcg gaacagttga agaaagacgg ctggagcttt gcttcccata
    67141 catggggtca cttgaatatg actcaagctt cactagaaga tgtaaaaaaa gacaatgaat
    67201 tgtggcagca agaagttgca cctatcctag gaaaaacaaa tattctgatt tatccatttg
    67261 gtgcagacat cagcgactgg cagccttatt cagcagaaaa tcaaaaattt gcttacttga
    67321 agagtcaagg tttcgatatt ttctgtaatg tcgatgcttc tacaccagct tggggacaat
    67381 taggtacgga tttctatcga aatgctcgga tcaatatcga tggcattcgt tttcaatctg
    67441 atttagacgg aaaaaatcct attcttgatc cattcatcaa tgtccatgat gtctatgaca
    67501 aagaagcacg cgaatctgct tgattttacg tcatttatga accaaaaaag ccgaactgac
    67561 attttatcgt cagtttcggc ttttttatgc tttatttctt cttcggtgcg attcccttga
    67621 tcaggacttc tgtccattct gctgtatatg gaagcagttt tgcgtatatc tccggcttag
    67681 aaattccttc taccattgct tgaaaataga ggatcaagaa ctcatccgaa tatttcaaat
    67741 ccacttttcc ttctttccgc cctcgatgga aaagatcaag catgatactg aaactttctt
    67801 gcgtatacgc atccatcgcg tcagaaaatt cttttttttc tttttttgta tagtatgtca
    67861 tcaaatcgat ataaaattgt ttgttcactc gattgagata acggattttg ttttcactca
    67921 tggcaatcag tgtttcttca aatggaagat tttccgcaat caacgctttc gcttcttgcc
    67981 ctaaatcatt cataaatgca aaaaatactt cataaatcag cccttccttg ctgccaaaat
    68041 atttaaaaat cgtcgcttta cttacctgag cgttctcagc taccttatcc attgtcactc
    68101 cagcggttcc tatgtctgtg ttcatcaagt caaaagctgc ttgtaaaatc tctttctttt
    68161 tctcttgtgc tctcttttca aaaccgttca tttgtagagc ctcccgtaat tttaaacttt
    68221 tataatatat atagttcaaa tttattctga ggtttattat aactatctat tgagaaagta
    68281 tcaagctagc aattccttgt ttatgaattc attgacagaa atttataaaa agaatattat
    68341 gtactaaaat aataaaaaaa gttttataaa aaggtataaa acctttttat taaactattt
    68401 aatcttgttt agtatatatt tttagaaaaa ggagtggggt ttttatggca gaaatcgtca
    68461 aagtcacaca tttgaaaaag agctttggtc gtttcgaagc tttaaaagat gtctctttca
    68521 ccgtacattc aggtgaagtc gttggattta tcggaccaaa tggttctgga aaatccacga
    68581 cgattcggac acttttaggg attataaaaa aagacggcgg aacggcaacg atttttgggc
    68641 aagatgtatg ggaagatagt ttatctatcc atgaacgtct ttcttatgtt ccaggcgata
    68701 tctctttgtg gggaaatttg actggaggag cgatcatcga tctcttcatg aagcttcatg
    68761 gaaaagggag tgctcaaaaa aaagatgaac tgattcaacg tttctctctt gatcctaaga
    68821 aaaaagcaaa aaactattca aaaggaaatc gccaaaaagt gggcttgatt gctgctttgg
    68881 ctgttgattc ggatctctat ttatttgatg agcctacttc tggtttagat cctttgatgg
    68941 aaacgatttt ccaagaagaa gttgaaaaat tgaaagaaca gggcaaatcc atcctacttt
    69001 cttctcatat tctcagtgaa gtcgagcgac tagcggataa agtcgtgatc atacgcgaag
    69061 gaacactcga tgaagaagga acactcgatg aattgcgtca tttgactcgt tcgaccatca
    69121 ctatagaaac agaagcagag aatagtcact tagcacaatt gaacggtgtc catgactttg
    69181 tgcaaaaagg tcacctcgct caatttgctg ctgataacga agctttcgat gtgattttag
    69241 ctgaagcgtc caaattaggt gtgaaaaaat ttgaatctat tccaccgact cttgaggatt
    69301 tattcatgag acattatgaa ggatgaggag gaagcgaaat gactgataaa ttttcacgct
    69361 ggtccaccct tttttctcaa aaccttagcc gtgactggaa aaagatcctg atatggctag
    69421 tcggtgtcac tgctttttct ggtggttttg tccctgcgtt tgaagaattg acttccggac
    69481 aaggattagc tggattatac gaaaccttgc aaaacccggc aatgattgca atggtcggac
    69541 caactccagc ttcgaatgct gctgagtata ctttaggagc gatgtatgca aatgaaatgt
    69601 tgcttttttg cagtttgttt gctgctgtga tctctgtttt acacgtggtg agccaaacga
    69661 gaaaaaaaga agaacttggt ctgattgaac ttattcgttc ctttcgagtc ggacgtcaag
    69721 caaactcttt agctgtcatc gctgaaagta ttctgatcaa tttgctttta gcaatatcga
    69781 tcagttgtac gatgattttt ttccaagcgg attcgatcac tgcagaaggt gcttggcttt
    69841 ttgggctatc tgtaggtagt atggggattt tggggagtgc cgttgcactg tttgctgcac
    69901 aactgatgcc aagttcagcc agttcgaatg gtctgtcttt agcgttttta ggtggacttt
    69961 accttctacg agcaataaca gatgtagctg attcctccct ttcgatgtgg aatcctttag
    70021 gctggtctta tttgacctat ccatttacaa ctaacaactg gattcctttg ctttatttgt
    70081 ttttgttcag tctaatcttg ataatcagtt catttgtttt agaggaacat cgagatttga
    70141 acaccggcta tatccccgaa cgtgaaggaa aaccgtatgc taaacgttct cttttatcct
    70201 tgcgtggttt attattctat ctaaataaag ggatgacaat cagttggctt atcacttttg
    70261 gtttgatggg ggcggcatac ggctctatct acgggaatat gcaacagttt ttagaaagta
    70321 acgacttaat gaaacagatg tttatccaat ctggcgtctc aattgaaaaa aactttacaa
    70381 gcacgatcat ggtcgtcttg atttgtctag ctgctatttt accaattgct agcatcaatc
    70441 gtttatttac agaagaaacc cacggacgta tgaatcagct gttcgcaaca aaaatttcta
    70501 ggaaccaact ttattggacc aatatgttac tagctattct atttgctata ttggcaatcc
    70561 tgattagttc tggtgcacta gggctttctg ctttggcttc gatgaaggat gcgaaagaca
    70621 tggatctatt gatgtttctt aaagccgggt tcaatttcct gcctgccgtt ttagtgttta
    70681 tcggaattag tggctttcta ttcggatggc ttcctcgaat cggtaaactt gcttatatct
    70741 acttagctta ctcctttgca ctcaattact ttggcggaat tttggattta ccagaatggt
    70801 tttccaaaac agctgtccct agctgggttc cacggatgcc aaacgattct tttgatcttt
    70861 ccgtgttcct tacgttatca tgtatcagcc ttgcactgtt cgtccttggt tctattggtt
    70921 ataggcgaag agacttacta gaaggataat aaaaaagcat gtacaatttc atgctagcct
    70981 ccaaatctgg gtcaaaaatc caacatttgg aggtcatttt tttatttaca agtcttctaa
    71041 ctgctcttta tcaatgatcc agacttctcg attctttctt tttacataac cattctcctc
    71101 taataattta aattttctag ataaagtttc gggggtcgta ccgataaatg cagcaagttc
    71161 tttcattttc attggcagga aaaagtgatc cttccctgtc gctttagcta agtcaagcag
    71221 atagacagcg actcgttctt ctactttttc catcatcaaa aaagttgtct gctgctgtac
    71281 ttttgttaat ttctcaacgt tgattgctaa aagtttcaga ctaagggcgg gataatgcag
    71341 taagagttcc gagaaatctt tttgtctcaa tatgcaaatt tccgtttttt ctagtgcttc
    71401 gccaaataaa gactcatttg gaacgcctag caactggctc tctccctcat atccgcccgg
    71461 ctctattact cgtaaaagct gttcctttcc gctagcagat aattgataca ctttcatact
    71521 gccggaggcc acgatcacta attgaggttc tccattcggc gcaagaatct gttctccttt
    71581 ttccacatag tgatgtttga caagtgcgtg tactttttct tgatcttgct gtactaagtg
    71641 attgaacaaa ggaacaaatg aaacacataa atgatcatga ctcataatct ctcctccttc
    71701 taaacagcta tttctaataa agaaacataa cttcccttat tccaactgat aattttcttt
    71761 tatcatagca taaacgtcaa gcgaaccaga taccagcaaa tagagtggat ttctttttgt
    71821 cccttgcttt attttccttc ttttgagacc ttaagtaatt gattcctatc aattttttaa
    71881 atatttactt tttgaataat caaaaacaaa ccttcactcg caagccgaga gtaaggtttg
    71941 tttttcgcat tgcttatttc gtgttgtcta ttgaaatttt tccgcaccta gcttttgata
    72001 tagtggtgct agttcttgta aggtttcagc ggttatttgc tcaacgtgtt cgatttcttg
    72061 cggatagaag aatagtttac ccatttgaaa ggttcggtct ctcatcgctt ttttcaactc
    72121 ttcttgtttc tttgtatcgg acaaatagtc agacaaagct aagtggtcgt caagttcatg
    72181 ttctggttgc ggccatatgt aataatcctt gatatcgact gttttgctcc actctttcag
    72241 tatcgcaagc aactggttat acgctgcaat gctaccatat cgttcttcgc ttttataatg
    72301 ttggtacatc aaatagattt ggtattgttt ttgattcaac aaaactgcta gacaggcatt
    72361 ctcgtcttga cgagattcgc ttcggtacgc gctccaaaaa tgactgcgta aattccagcc
    72421 attcgtccag ctttcgattt taggtttgct catgccgtac atatcaggca aaagtgcagc
    72481 tgtctggagc tgaatctctt tccatttttg ccagtgttct ttatattctt tcttgatttg
    72541 ttcaatctta tctgttggat ggctctcttt gagctgtttg aatgcgtaga ttggtcgatc
    72601 aaacaactga taatctgatt ttccaagttc cattgtttcg cctctttccg ttatcttttg
    72661 tattctacct gaattttgcg atatggacaa atagaggttg tgaaaaagct gttctgcatc
    72721 aagtcataag aacagccaaa caaaaaactg cgatgagcat caccgcagtt ttattgaact
    72781 taaggttgat tgatcacatc taaaagcgtt gaaaataaaa tggcactaga agcagctcct
    72841 gggtcgatat gtccgattgc acgctctcct agataggaag ctcgtccttt tttagctacc
    72901 aagactttgg tgtgttcttt tgcctggtca atgatttctt ttgttaaatt ccctgccttc
    72961 aatgcctctg tgactggcag ccaaacatct aacatcgttt tatcgtcagc ttctgccttt
    73021 cctcggcttt gaatacccgc tgttccctgc tcgatcactt cgcctaattt ttcttgagaa
    73081 tcaatagaat cgacctcttt tgtcgctttc atcatgttca tgaatgcaga tccgtataac
    73141 ggaccagaag ccccaccgac tttcgatatc atcgccatag agaatacttt gaatgtatcg
    73201 ctgattgttt caggatgatc ggcttgaaaa gcttcttcat aagctgaaac ccctcttccc
    73261 atgttatttc catggtctcc atcgccaatc ggggtatcta gatcactgag atattgtttg
    73321 ttctgattga ttgcctctgc aaattgcgtg agccattctt ggatttcttg aacggttagt
    73381 ttcatattat caccctttta tcttgagatt ttttaccatg aaatcgtctg tacattgctt
    73441 tctaaagaat ctttccattg tgtctccgtc aagtcgatca gtgttagaga aagaccttgc
    73501 atatctagtg aggtcataaa attaccgact tttcggaaaa ctacattgac gcctctttct
    73561 tccaacagag ctaaaacatc attcatgaac acgaactgtt ccatcaaagg cgtcccgccc
    73621 attccgttga ctagtacacc tgcttctttt ggtaaattcg agtaatcatc taaaatctta
    73681 gtaacaagtt cctttgccag tactttagaa ggctgcatct tttctcgacg atagcctggc
    73741 tcaccgtgga taccaacacc atactcgatc tcatcttctc ctagttcgaa tcctggtttc
    73801 cctacttctg gtacagttgc tgctcgaagc gctagaccga tcgtcttagt tgcctgtacg
    73861 accttttctc ccagttcttt caactcagct agggaagcac cgtttcttgc tgcatcgccc
    73921 acgattttgt gtacaaggac agtacctgca actccgcgtt ttccagcagt atacgtacta
    73981 ttttcaacgg cgatatcatc atctacgatc acactttcta cttcaatatc gtccatagca
    74041 gctaattcct ttgccatatc gaagttcaat gcatcccctg tataattctt aacgatcagc
    74101 aaaactcctt ttccactatc ggcttcctta atagccgtct gaatctgatc tggagttgga
    74161 gaagtaaaca catcaccaag caccgctgca cttaacatcc cttcccctac aaacccggca
    74221 tgggatggtt catgtccgct tcctccacca gaaaccaaac caacttgttc cgctttctga
    74281 ttcttggcta caacacggga agattctaca cgatgaacaa gttctggata gctttttact
    74341 aatccttcca gcatttcttc gacaatattg cttggatcat tcatgatttt tttcaattta
    74401 ttcccatcct ttctttccgg cattttgtgc ttctgttaag atttgctgag tatcatctgt
    74461 tataccggca gtgatggctg ctgcaaaagc tccttccact aaaggcgcat ccaccagatg
    74521 ataacgttgc tgctgctctt cttccaacat gtcttttgcc agttccgagt tcaataccgc
    74581 gctaccgata tccgcaaaaa caaaataact agctgcatcg cttgattcat tgactgcttc
    74641 tacgattttc atcgggtctg atccgatctc gccatttatt ccgccgagag aaaaaatttg
    74701 cacctcttcg gatttttgca tctgctcaat catttcttta ataccatctg ttactttttg
    74761 actatgagaa ataagcaaaa tacttttttt cacaagaatc ttcctttctt ttacatagtt
    74821 gctaacgatt ggtttcgctt tcattaatga acaattcaag tatataataa atttctaatt
    74881 ttgggaaaat atgtgtagtg cttcttgaag tttgttatac tttaattaaa agagaggagt
    74941 gatcgaatga ttacaacaac aacgaacagt gtagagggaa aagccgtagt agcttataaa
    75001 gggatcgttt ttggtgaggt cattactggg atccatgcat ttaaagactt ggaggcgggg
    75061 ttgcgaaata tctttggcgg ccgctcaaaa agctatgaaa acgaactaat gggtgcaaga
    75121 gaagaagcac ttgccgaaat ggccgaacgt gcaaaagcat taggagctga tgcgatcatt
    75181 ggcgtaaaaa tggactatga agtattaggc agcgacaatg gcatgctgat ggtcacatgt
    75241 agtggaacag ctgtctccac gagataagta ggggacatct gttttaataa ctaaagagag
    75301 atgggacatg tagtggcact caagaattag atttttcagt ctaacttcga ggtactctac
    75361 cttcccatcc tctctttttt atttcttttt gcggatgatt ccgtaagctg ctgcgactaa
    75421 cacacccgcc cctgctaagg aataactgac agaagctgtt ttttcatttg tttttggcaa
    75481 agtctgactt gttgatttgg atggctctgt cgtttgctga actgtttctg tcgtgcttcc
    75541 agaggtcact gatttttctc ctgccgaatg caaaatatct tttattcgtt ggaaactagc
    75601 tgctaagtca gctttttttg cttcaaaatc attagcaact gtttgtgctt cgctgatctt
    75661 tccgtggatt gcttgactgg ccgctgtgat atccgcttga gtttgactta gctttccaga
    75721 agctgtttga gcaacctgat ttaattgctg attgaattct tttgcttgtt ggatcttccc
    75781 gtcaagatct gtttggacag cttccgcttt tgctttcgct tcggcaagct ttttctcaag
    75841 ttgtgcacgg tcatttccca ctttattttt aagatcagct aaatcttgat cgattgctgc
    75901 ttggtcttct ttcaatttgt tttttgcagc ttctaatgct tcttcgagtt cttttctttt
    75961 attttccaag tcttgcaaat gatcgatttg gtcagaagag gtagaactat ttattgatgt
    76021 atcttctgaa gatgaaatcg tttctcctga attatctgtc ggtacattgg aagtcatttc
    76081 tgtttccgag gcaccgtttt cagcaccaga agagcttatg gttgttgaag gttctgtttg
    76141 agcaggttca gaagtttccg caaaagctgt ctgtcccaat aagaaaatac tggaaacagc
    76201 aattggccag attaacggct tcattctttt tttcatcact tttcccatcc ttactacttt
    76261 ttaactaatt tcttattata agcaaaaatg tttggaagaa ccataaacac aacaggctgt
    76321 ttgtctaaaa aacggtgcat cttttatttt tttgtacgtt ccgggctttt ccatgtcccg
    76381 cttccataac ctaaaatcgt tttgactgct ggaatgaaag tcgtcacgat cgttatcgcg
    76441 ttcactatcc agaacaaaag catatatagt ggtgcgaaaa gaaggtattt caattttctt
    76501 cgtcgatcat ctacaatcag cgaagctagc aactggaaaa atcctgcgac catttcaaag
    76561 cagatgaaca caaaactgaa tgtaaaagta atataaatct gttcgtagtt tccttgatat
    76621 acataatgcc cgattaatcc agcaaaaaga actacggaaa tacaaaagaa tattgaccag
    76681 acaatactca atgtttgatc aatgaagatg atcgttcttc caatatgttt aaaaggatgg
    76741 agcatcactt ttttgaaatt cgtcagccag acttctgttc caccttttgc ccaacgtttt
    76801 ctgtgtcgat atagcatctt caacgtcaca ggtacttcca tgaaaaagat aatctcagga
    76861 gcaaaaacag ataaccagtc atttaactga tgatcccaag caatactgat gtcttctgtc
    76921 gctcgatctt gtctgaatcc gcccacatcg atcaaggctt cttttcgata catcgtattt
    76981 gctccactgt acgcataaag accaccgaaa acagctgact gtgttcgttt aatgatgccc
    77041 acgatacttg agaattcaac cgtttgagat tttgcaatca atttggtacg attttggaca
    77101 tccatatttg cagtgaccgc agcaatatgt cttgcaccag ggcgaatgaa ataattcaca
    77161 tacctaatca gtgcatctgg ctccggtacc gtgtctgcat cattgcttaa aatcaatttc
    77221 ccttttgcaa aggcaagtcc gatattaaac gcgtgggctt ttcctttatt tttttcgatc
    77281 cgaaccacac gaagattagc gtattttttc atcagtctag ctaatatttc tggtgtttgg
    77341 tccgttgaac catcgtctgt cactaatacc tcatagttgt gataattcaa tttcgtcatc
    77401 aaatactcga ttgtatattc gatcacaatt tcttcattat gggctggcac catgatcgtg
    77461 atgaacggtt cgacactcaa gggaacatct acccattctt tttgcttatg tttgtataaa
    77521 aacacataac accagacacc aataaaccat acaaatccgc ctaaaatcgg ataagaaacg
    77581 agaaaaaaat acataataga caaacaagta tctaagaata aattacctgt atgggtgatt
    77641 gagatcattt cagttccacc ccattttctt tatacaaatc tgcaatgaat gtcgtatcta
    77701 gatttttctc ttcggagaca gaataaaaac ggaccgtttc tctctcttct tttgttccaa
    77761 aacgttccgt atacacttct tcaagtaatt cttgtctttt tttcaatttt tcatcttcat
    77821 attcaaaaga tttatttact tttttgtata gatttcgatt gttccaccta gtcaatacaa
    77881 atgaggtaat caggaaactg aaaaaaatca atacaaaaaa gccgattaag aaatacagca
    77941 ttttcttttc tgataaaaaa gcgtacaagg aaaaatcctt ggctctttct ggtctcgtta
    78001 atgggaaaaa gacccatata aaaggaagaa caacgatcat ccatcctaaa agagccacta
    78061 acgtttgcca aatctttaac cagtaattac cttttgaaaa atacttatct ttcaccaaaa
    78121 ttgatgcaac ttcttctctt gttacttttt tcatatcctc acctcacgtt tttttgttac
    78181 gacgtaggag tttaccatac atttcatttc ttttcacata aagtgttcct aaatttttcc
    78241 taagaaaaag aacaaaacat ttctcatcag caaatgaaaa atattttgtt ctttttatca
    78301 gttgttcttt ttatttttta tctcttaatt atgttgctct ttccaatacg acaatgcttt
    78361 tcattcctct agatatcgcc gtgtacattt gtctgcgtct tctttgctca tctccatcgt
    78421 tttcaaatgg atataagatt acatgatcaa attccagtcc ttttgctaga ctcaaaggaa
    78481 tgatttggca gttttctaat ccatatgaat tttttttaat cgattcttgt agcgtttggg
    78541 tttcctcttc tgtttgggtg ataactgccg ctttttctcc ttttagagaa accaaaatct
    78601 cagctagtat tttcaacaat tcttcagagg atcgaaaaga gcggtattcg ggttcttttc
    78661 cttgcggtcg gatcgatacg atgtcgatct tctcttgatc cacagcatag gttttgaaaa
    78721 gctctgtgat tgctccgcta gaacggtaac tatttctaag atcataccga tggatcggca
    78781 gatggtagtc gtcgaaacaa cgcataatat cagcaaaagt agtggaagaa ttaaagattg
    78841 cctgattctc gtcacctact aaagtaaagt gagtttttgg aaataattcg atcaatagac
    78901 ctaattgtgc cagggtataa tcttgtactt catcaatcaa tagatagcga aatgctggta
    78961 catttacctt ttcaacaagt aaatgacgaa tagctaatat aatgactgct tcatcaacag
    79021 tacgtgtggg ttgccaagca taagctgagc catatcgttt ttcataaatt ctttcaaaca
    79081 actgatgctc atccacccat gccatttctt cgatttgtct ggttattttt cgatattttt
    79141 tttgtaaaag tttccttgca taagctacaa tactttgttc cgaatcatca gaaatcaatt
    79201 taccgaataa ttccaactgc ctatcttctg tcaacgagag tacctgattc tgcacagcag
    79261 gactttttgc ttgtttcaaa agatgattct tccattcgct catcagtgcc tttttcgttg
    79321 cttgaagcct atcgatcaat ttaggatgag caggaacttt ctcatatatt ttttcgatcg
    79381 tttttttaga aaggactgtt tttccatttt tagtaagtcc acggataaaa ttcggatgat
    79441 ctaaaaacaa aggatctgat tgttttagat tatcgataaa ctttttgcta cgcaaacgtt
    79501 ctgtctgctc cgatacattt tctcccgtga tacgtttgaa ataagcctct tcaccttcta
    79561 tttcctctgc tgaaagctgt gaaaccagct gtatgatggt tagattcagt ggatttcttt
    79621 cacctaatga tggtaggaca ttcgaaatat agtcgataaa ccgatgatta ggtgatagaa
    79681 tcagcaagtc atcggatgtc atcgtatctc gatataaata gagcagatac gcaatacgct
    79741 gcatgatagc agaggtttta ccacttcctg ctacgccatt taccaagata ttttgacttt
    79801 tcacatcccg aatgattgtg ttttgttccg attggatcgt agcagtgata gccttcattt
    79861 cattggtctc atcttggcct aagacttcta ataatacgtc atcttgaata gctacagacg
    79921 tatcaaaaaa cttgaccaaa cgatctcttt ccaaaagaaa ttgtcgtctt tctttaataa
    79981 ccgtatcgat tttattttga cgaaccgtat aagaagatgg accaatcatg ttattgtaaa
    80041 aaagttcggc aattggtgaa cgccaatcat aaacaagatt ttcatctgca tcatctgtga
    80101 aatttgctgt accgatataa aaggattctt ttggctcatt atccagaaag tctacaacga
    80161 cttttccaaa gtagggactt ttcaataaac gttcgatctt tgctagctga ttttctgcgt
    80221 ttttctgttt gatattcatc tgatcgattt ctctattttt catttcaatc atcgaaaaag
    80281 tatccaggtt atctaagtaa ctgtcaaaat tcaatcgaag ctcgccagcc atttctttca
    80341 acgactggtt tccttctatt tttgccgtat ccatcgattc ccgcagttcg ttttttttat
    80401 caattagctt ttgatacacg ttcttcagat atgcttcttc taactgtttt tgtgtgttga
    80461 ccatacacat tctcctcttt ttttaaagtg gtggaaccga ttagtttacc tcttttaaga
    80521 gtcgaatgca agctttttaa cgagtatcat ttgttaggat atatcagttc ttcaggtcgg
    80581 acagtttgat ataaagaaaa tgcaccatca aggtttttta cgtggtagcc agactgcttc
    80641 aagattcttt ccgctagata gctacgtaat ccgctatgac agctgacgat atattcttgt
    80701 tggctgtcta attcatttaa tcggtcccga agttcattca aagggatatg tttggcttcc
    80761 ttgaatttgc ccttttccaa ttcttctgct gttcgcacat ctaataaaat ttttcctttt
    80821 gctagttctg tcggcaattc atgccattgt acggtttcac taagcccttc tgcaatgttc
    80881 attgctgcat atcctagcat gtttactgga tctttggctg agccaaatgg cggtgcatac
    80941 gtcaattcta attccggtaa atcgaagatc gtcaaatgcc ctttgattgc tgttgctaaa
    81001 atatcgatcc gtttatccac accttttgcc ccaacacctt gtgcaccata aatctcgcca
    81061 gtctctggat ggaagatcaa cttcagtaaa atatctgttg cttctggata atagcttgca
    81121 tgatcttttc ctgatacatg aatgacttca aaagacagtt cgttttcttt ggctgttctt
    81181 tcacttaaac cggtggatgc ggcagtcatt ccaaacgttc gtataattgc tgttccaata
    81241 ccgccttgat ttcttcgaga aattccggaa atcgtgtccg caacttgtct accttgacga
    81301 ttggctggtg aagcaagaga aataagtgca tcttttccag tgatttgctg ttttacaacg
    81361 atagcatccc ccaccgcata aatatcagga atattcgttt gataacgttc gtctactagg
    81421 atacccccgc gtatgcctaa ttcgattcca gcggtcactg ctagctggtt atcaggacga
    81481 acaccaatgg ataaaataac taaatcagca gggatggtac gcccatcatc cagtcgcaat
    81541 cgtttcccgt ggtcttcaaa tccagcgaca gcatgagaag taatcacttc tactccttga
    81601 tgagacaatt ctttttctat aaaagctgcc atttcttcat ccagtgttgg aagaatatgt
    81661 ggtgcttgtt cgatcaccat gacttctaaa cctcttcttt ttaggttttc tgccatttcc
    81721 aatccgatga atcctgcacc aacgatcacg gcacgctttg gctgtttttc taatgcatgc
    81781 atcactttat cgatatctgg tacatttcta atagaaaaaa cattgtcggc ttctgttatc
    81841 cctggaatcg atggaacaac tggttttgca ccaggagata aaaccaatgc atcgtactgt
    81901 tcaatgtgtt tttgaccctc atgtacgact tctaccgttt tgttttcggg aaagatggct
    81961 gtcacttcat gatttggacg cacatctaaa caaaaacgag cggataatga ttctggcgtt
    82021 tggacaagaa gattttcccg ttcactgatt tctcccgaaa gataataagg caagccgcaa
    82081 tttgcaaaag aaacatacgg tcctttttca aaaacaacga tttctgcatc ttccattaat
    82141 cggcgaagcc gtgtagcagc agacattcca cctgctacac ctccgacaat aacgattttc
    82201 atcttatttc cctcctactg ttcgatcaaa ccattgattc atgccaccac gtacattcac
    82261 tacatcataa ccagatttct ttaattcttt agctgcttgt ttactcctca tccctgattg
    82321 acaaatcaca tagacggtct tttctttgtc tccatcatat ctgtttatac tttgtaatgg
    82381 aacattttta gctcctttta tatgcccgcc ccggtattct gcaggtgttc taacatcaag
    82441 taacgctgga ttttccaaaa gcttttcctt taattctggg actgttatcg attttattga
    82501 ttgaaatagc aaattcattt tctgcacctc atttacattt ttataaatac ctcatggggt
    82561 atattaaaca ggagctttat taatatgtca accaaaatga ctcttcattt ttttcactac
    82621 tttttatatt tgcataaaat accgataagg ggtattatct aattataaaa atagaggaga
    82681 atgtgttatg gagattgata ataaaaatat cctgaaccgt ttgaaacgta cagagggaca
    82741 aattcgtggt atccaaaaaa tgatcgaaga cgaaaaagaa tgtatggatg tcatcactca
    82801 attaagcgct gttcgttcta gtattgatcg cgtaatgggc atgatcgttg cagataatct
    82861 gaaaaattgc tttgagaaac ctgatagtaa tccagaagaa caagcggcta aactagaaca
    82921 agcgatcaag atgattatta aaaagtaaag gaatgatttc aatgaaagtc gttttccata
    82981 ttgatgaatt agaaaaatgg tcagaaacag ggaaaaacgt aaaaaacctg ataaaagctt
    83041 ctccagaaac gacgattgtc gtatctgtaa atggcatcgc gattaccggt tatctagact
    83101 ctgccaatgc agaattctta gatttgcaag gagtcgtttt tcacgcttgc gccaatgcta
    83161 tgcgtgctaa tcatatttca gaaagttctt tacctgaaca agtaatcgtt gtaccagcag
    83221 gtgtccttga tttagttgaa ctgcaatccc aaggttatgc ttatatcaag ccttagtcag
    83281 tagaggttgc gacagaagtg atcagcttca agaaatttcg gcttattttt cgaagaagct
    83341 acttctggaa aaccgtgaat caaaggttgt gaaaacgctg tcctgcatca agtaataaga
    83401 acagccaaac aaaaatagct tctcatattt ttcgtttggt tgggcttatt accgcagatg
    83461 caagcttttg aacaccgttt aaggaagagg ttgtgaaatc agcggtttga cctaagtatt
    83521 agagaaaaaa acgggaaaat agctcctcat attttgccgt tttttgagct aataccgaag
    83581 gtcagctgat tgaacaccgt ttaaggaaga gattgtgaaa aagtcgctta gtttcgagta
    83641 ttaagaaaaa ttttgtgaaa atggatttct acatttttgc ataaatttgg cttaataccg
    83701 agaaactggc ttttgaatac cgtttaagga agaggttgta aaatcagcgg tttgacctga
    83761 gtattagaga aacaaacgga aaaatagctg ctcatatttt gccgtttttt gagctaatac
    83821 cgaaggtcag ctgattgaac accgtttacc aagaggttgt gagcctacca tttaactccg
    83881 aatcacaaga aataatttca aaaacaactt ctcctgtttt tggttcggtt ggcttattat
    83941 cgagaagtag ctactgggag tctatttatc aaaaaaagga atgcgacata ggtcacaccc
    84001 cttttttatt ttctttgaaa catctttcgg tactcgacag catacaagac aaaagattgt
    84061 aaaagaccgt aagtgatgac ggcaaaaatg actctcaccc acagcgttgg taattctggg
    84121 aaaagcagca aaaggatcat gccaacatag atgacagcag tcgctacttt gccataccac
    84181 ttcgctccac ttaatttgat atttttagat agcccgatta atccagctac aaacataaag
    84241 ccttctttta tcaagaatag accaaacaac agccaaagcc acggtcgata ccatgccata
    84301 gacaaaatca gaaaaagttg tgtcaattta tctgcaaacg gatcaagcac cttccctaag
    84361 tctgttacct gattatatct ccgagcaatg accccgtcta gataatcaga caaacctgag
    84421 acaacaatga ccgctcctgc tatataaaat gactgtctca gcacaaaata aagataaatc
    84481 ggcagcaaaa aaatacggaa tatcgataaa agattcggaa tcgtttgtat ttctttacgc
    84541 cagtctgtgc ttaatttcat tttatcgccc tacctcctca gcactctttt ctttttattt
    84601 tagcttacac ggggaataaa accaatgatt tcactgtttt tatttttctt tacagacact
    84661 gatattatga gctaatttga gataatcact tgctgttttc atcgtttact cttctttctt
    84721 cctgtcgttt gcgcatttct tttactagta ctttatgagc tttttttgat cctcgctcta
    84781 ggtcacgctg ccgtttacgt gttttataat cgataaacag actgtctaaa tcgtaacctg
    84841 tcgggtacag atcttctgct gaacgcacca gacgaacacg ctgacgaggc acgtggatta
    84901 tttttttgtc taaatataca tcaatcgtat cttcacctat atcttcataa actaacgctt
    84961 ctttttggta ttcattgaca aatatccgat caccttttgc aaagagctga cgttcttttt
    85021 tattctcctt cctatttgta actgctgtct taggaagaaa ttcttttttc tgtgtccgat
    85081 aggctttttc cgccaagtaa cgctctgctt gatggatcaa tttcatcgac atatccattt
    85141 tatgagcaat ccacaaagct tggctttctc ctgtttcttc caacccgcaa ctgatattta
    85201 ggacgaagtg cttcccgatc gaaagccata gcagccggca caaaatcttc atgatctccg
    85261 gcgaatttct tgatttctcc ataatgggtc gtggcaatga tcaatgcccc ttgctcatac
    85321 atagattcca tgattgcgat cgctagtgca gccccttcat ttggctctgt tccactgcca
    85381 atctcatcta aaagtaccaa cgtattcctt tttatctttt tcaaaatagc agcgatgttt
    85441 tgcatatgac cagaaaatgt actcaacgca ttttctaaat tttggtggtc accgacatcg
    85501 acgaaaatct catcaaagac agcaagctct gtcccttctt ttgccggaac ttgcataccg
    85561 aacatcgcca tcaaagtcaa taaaccgact gtcttcaaca cgactgtttt gcctccggca
    85621 tttgctccgg tgataatcaa gcctcgatag tctttaccga tttcaacatc caatggtacc
    85681 gcatgctcag gtaaaaatgg atgtctgcct tgcttgataa tgatatgttc cgatttattg
    85741 attctaggtg tgcttccatt gatttctcgg ctaaattttg cccgagcaaa gatgatatct
    85801 aacaccgtga tcgtttcgat caataaatcg atcacttctt cgttttctgc gatagcacca
    85861 gtcaatgccg ccaggacttg atattcttca ctgatttctt cagcttttaa caactgatag
    85921 tgttcattca atttagaaat cacagttggt tcaataaaaa ccgtcgttct tttatttgat
    85981 tcatcgataa tcgttccatc tattttattt ttatagctgg ctttgatagg aatcgtatag
    86041 tattcgcctt tttgaacaat cattgcttct tggatcattt ctttgttttt cggatgacgc
    86101 aagaatttca gtaattttga ctggatttct ttttcgatta gttgtaattg tttgcgtact
    86161 ttacgcaaat tacgtgacgc gtcgtcactg actttattat tttttatttt ctggtcaatc
    86221 aactcttcta catttatcaa atcaggaaga tgtttgctat aagcaaacaa caagggtgtc
    86281 tggtattgat tcttatcaaa aaactttgtg atcatccgac tgcttctaag aaaatccgca
    86341 tattcgatca aatcggttgg ctgcagaatc aacccttttt tgacttgttc tgtcagtgca
    86401 tcgatcctcg gcaaccccat aaatggaaca tgctgactgc tttctaagat caagcgtgct
    86461 tctcttgttt cttcttgacg atccaagaca gtttgtaggt ttgtcgagac cgtcatatcg
    86521 ctgattcgtt ttttgctata gtttccaatt gctcttgctt ggatttcctt tttgatccga
    86581 tcaaattgta attgactaat catttcgttg tccatattct ctccttttct tgaagagata
    86641 cctgtcccaa aataaataag ggcctagaga taaataaaag gatagaacac agcttaaaaa
    86701 agtcatgtcc tatccttatt agaatacaaa gatattgttt tcctgacagg taccgtttca
    86761 taagcacacc aaataaactt tgtttattgg ctattcgact tactgattag ataccagcta
    86821 cactcacaaa acatacccca ttcttctagg tcttatttat tttccactta actatagcaa
    86881 gacacatttt ggttgtcaaa ctttagtatg ttttatagat ttacaccatt aatgaataga
    86941 cacctaccgc gataattgct ccgatcattg gacctgcgat tggtacccat gaatatgccc
    87001 aatctgaagt tcctttatta gctattggca ataccgcatg tgctaatcga ggaccaaagt
    87061 cccgagcagg gttgattgca tatcctgttg gccctcctaa tgaaagaccg atggctaaga
    87121 tcaatccacc tacagcgaat acgttcgttc caggtgcaaa ttcgttttgt ccaaaagcta
    87181 gcaacccaag aactaataca aatgtaccaa ttaattctgt gatcacattt gctggataat
    87241 tccgtatagc aggacctgtt gcaaacgttc ctaaaatcgc gctttcatct ttcgttgcat
    87301 tccaatgtgg caaataagaa agccaaacaa gaatggcccc tgcaaaagct cctaaaactt
    87361 gcgcaatgat gaaaggaacg acaagattcc aactaattgc ccctgtcatc gccatagcta
    87421 gagagactgc aggattcaaa tgagctgggc ccatgaatcc agaaatatag acagccattg
    87481 ttacagcgag tccccaacca aatgcgatga caatccagcc agaagcctgt gctttacttt
    87541 tgtttaaatt cacagccgca cacacaccat ctccaagcaa aactaggatc agtgtcccaa
    87601 taaactcccc catgatttga gtcatatcac tgttcattta tttctccccc tttagttccc
    87661 gcagatctga ttctgcaatc aatgcttcta aacgtttagt ctgttgcgcc ttctcctctt
    87721 ctgtccattc aaaatatcca gcaattgcat cgatcacagg ttgcttgatc tcatcaagct
    87781 gatctcgttc aaacaacaga tgatttgtac gacgaatgag ataatcgcct ggggccaaaa
    87841 ccatttcttc ttctaaacca taacgtaacc gagctgactc agcgagactc aaaccaggat
    87901 aaggtgccat ttcttttgct aattcaaaga tacgtcgggc attagtcccg taaaaatcag
    87961 cgatataagt agcatcttct tcctctaaac cggctgctac tccttctttt gctaattctg
    88021 tcactgtttc ttctaattta gttggatcga agtttccacc tgaaatctga tattttttag
    88081 agtcgatttc tttcgtctct attccgtatt cttcttttaa taattggcgt atcaatcgca
    88141 aagctccttc tgccatctta cggtaatcgg tgattttccc accagataaa gtcagcaaac
    88201 catctggttc tctttctaaa gaactacctc tggaaatcgt agaaggtgct ttttcatcac
    88261 ggctgttttc caaatggttc aacacgtctt ctacttcagc acgagaaact ttgttttcct
    88321 tatattcact tactgtatcg acaactttat tgaagctctt gtctgaaatc gatccattat
    88381 ctccaccatt gtaatcagaa ccagaattac caatcaacag tggacgaagt cctgcccagc
    88441 ttgcttcgat atcattcaat gtgatatttg cttctggata gcgatgattg atcacatcca
    88501 atagataatc tacatcttct tgtgtgactt tcggatccgt aaaatctcct tggtaatccg
    88561 tatctgtcgt accaaagtaa gtcttgtttt ctcttggaat agcaaaaacc atccgcccat
    88621 cttgctttcc tgtatcgaag tatgtgggtt gcggtacagg cagttttttc gcatctacaa
    88681 ctaaatggat ccctttagtt ggacgcattt taggagagac tggacgcgta aaattcaagt
    88741 ttcgtacttt atctacccaa ggaccactcg tattgatcac taattttgct ttgatttcga
    88801 tcacttcatc tgtcagcagg tcacgagctt tgacgccaac gatttgatcg ccatcatata
    88861 aaaagccaac cgctttcatt ttacttacta gataagcccc atcttcggca gcctttttga
    88921 tattatcaat cactaaacgg gcatcattgt tgcggaaatc tagatagaca cccgcgcctt
    88981 ttagcccttc ttttttcaaa aacggttctc tttccaatac ttcttctggt gtcagcgtat
    89041 aattctcata tttggttcct gtcacatttg ccagtttgtc ataaaggtcc attgctactt
    89101 tgaccgagaa catattgaag gttgttgctc cttcgccctc atagattggc aaaagcattg
    89161 gatctggttt tgggatgtgt ggggcaatac tttgtacgac tgcacgttca ccaactgtgt
    89221 cagctactac ttccacatca aatgtcttca gataacgaat accgccatga acaagtttgg
    89281 tcgagcgaga ggaagtccct tctgcaaaat cctgcatttc gatcaatcct gttttgattc
    89341 ctgatgctgc tgactggact gccacaccgg caccagtgat tccgccaccg atgatcaaca
    89401 gatccaattc ttcttgctgc attttttgaa tgctatcttg tcttgtcttg tttgaaaaca
    89461 tatggatttc ctccttattc aaatgcttgg gtagctttta ctgctttttt ccaaccgttg
    89521 taaagttttt ctcggcgctc ttcctccatt tgaacttcaa atagcttgcc agcttcataa
    89581 aattcgcgga tctcgtctgt atctttccaa taaccgacag ctaatcctgc taaaaatgct
    89641 gcccctagcg ccgttgtttc caagttttct gctcgttgga tcggtacatt caagatatct
    89701 gtttggaatt gcatcagata ttcattgttt gctgcaccgc catctacttt caatacagga
    89761 atcttgattc cagtgtcatc ttgcatcgta tcgatgatat ctctcacttg ataagcaatc
    89821 gactgtaaag tagctttaat gatatcttct tttgtcgtac cacgtgtcaa gccaaacata
    89881 gcccctcgtg ctgcctgatc ccaataagga gcacctagcc caacgaatgc aggaacaaca
    89941 taaacctcat cttcgcttgt agagcgtttc gctgcgtctt ctgaatcgct agctttttgc
    90001 aacatttgaa gtccatctct cagccattga atcgaagaac cagccacaaa aatacttcct
    90061 tctaaagcat agtaaacctt tccatttatg ccgtaaccaa ttgtcgtcaa taaattattt
    90121 ttggataatt gcggtttttc accggtattc atgacaataa atgaacctgt accataagta
    90181 ttcttgacca tgcctggttc gaatgccatt tgtccgaata atgcggcttg ctggtctcct
    90241 gccatccctg aaattggtgc ttcgcttcca taaaaatgat aaccttgcgt ataaccataa
    90301 acttctgagt tagaagtgac ttttggtaac atcactcgag gaatatttag taaatctaaa
    90361 atctcctgat cccaatctag ctgatggata ttgaacaaca tcgtccgact ggcattcgag
    90421 taatcagtaa catgtgcttg tccatcggtc agtttccata ctaaccaaga atcgatcgtt
    90481 ccaaacatca attcgccttt ttcagctcgt tcttgcgcgc cttctacatg atcaagaatc
    90541 cagcgtatct tcgttgctga gaaataggca tcgatcacta accctgtttt ttcgtggaaa
    90601 aattcttgat agccttcatt ttttaagtta tctgcaattc cagctgactg tctggattgc
    90661 caaacaactg catgatagat tggacgtcct gtatcttttt cccatattac tgttgtttca
    90721 cgctggttcg ttatgccaat cccagcaatc tgatttggtt tgatccctga ttcgatgaac
    90781 gctccggcaa taactgactg tacagaattc cagatttcat ttgcatcatg ttccacccaa
    90841 ctttcttgcg ggaaatattg cgtgaactct ttttgagaac tgccgatgtg tcttcctttc
    90901 ttgtcaaaga taattgctcg tgagctagtt gtaccttggt caatcgccat gatatacgtt
    90961 gattctgtca ttgtgattcc ctccattata tgcaaacgct ttctttaact actcttatca
    91021 tactactgtt ttaaaaaaag aaactgtcaa aattttattc tttttttctt tttttgacag
    91081 ttttttatta tgaagctatt ttttaatcaa ttcttttctt ttttcagcgt ctctattctt
    91141 cttttcaatc gaataatgtc gtaaggtgac gcaaattctg acagataata ttcacactgt
    91201 tttttgtttc tctggcaaaa tgtgatcacc aaatcatatt ctttaccttc ttcataactt
    91261 ccaatctcaa taccgccgat tcccctcagt tctctgatca gatattgttg aaaagcaata
    91321 cgataaatcg gcagactttc taaatcgatt ccaatcacta ctggttttgc aatataaaaa
    91381 tcgatcatca aaagaacatt ggcatactgg atcatcaggt aactaagctc actatcttca
    91441 gctcttttcc tcggcaattg ctgcaccgct gtttcctgta atttcgccag taactgtgtt
    91501 aaggaatgcc cgagcatttt ctgctgctgt tccaaaagcc gatcacgctc atagacttcg
    91561 atctttccaa caaagaaaaa ccattcattg ttgatctggg aaatttggta gcctactgcc
    91621 ttttcttctt tgattttcaa acgacgagga cgataatgcc ataacatcgt ttcccgaatg
    91681 taagtatcta aaacagcggt tggtaacttt ttgctccgtg tcaaatcata ctgataaaat
    91741 atttcttcgt ctactatcga aaaagaaaca aaaaagctgt aaaacatcat tgtttcgtat
    91801 acattcagtt ctgctgctgt tcgactaaga tgcatcacga tgattggatc gattgcttga
    91861 taaagccggt ccgactgata gagtaatttc ttttctttca aagttgcgta cgtacttttt
    91921 tcattcaata gtcttttttt cgtaatcccg agccagcaag caatctgtgc ttctgctgat
    91981 gccgtaaacg ttgtttctaa tacacggtcc aatcctagaa tgaacggtct tttttctggt
    92041 gtattttgca aaaaaagcgg tcgctgatct tccgggatat attggaatag ttcataataa
    92101 aaatatcgta tctgtagctc ttctccgtat aattgaccgt ttttgatttg taatccaaat
    92161 tcctctaaga gctggttcaa ttctttgatc tttcgaaaaa cagaagactc actgatatta
    92221 aacgctgtcg ctaatcgaac gatggatact tgctgatgtt ctaataaata gacgagcatc
    92281 ttaaacttca agctttcctg caataaaaac aatagaattt tttccaaact tacatcttcg
    92341 tccaacacaa gcagcgcctg atactcattt cgctgtactt ctactttttt ttccatcatt
    92401 cggccaatca attcaatatc ttccaaatat tgatcaaacg aggcttttga cagttggaca
    92461 tgctctctca aaccattgat agaggctgtt cccccttgca gaacaaggtt tcgcaaaatc
    92521 aggatctgta actgctcttt tctttctaat aatgcttcaa tcttcatagg cagtattcgc
    92581 cacctttgac cacaccatat ctggcacacg gtattctggc tggaaaaata gctcatgttc
    92641 ttctaactgt tcaaacgtcc atccttcttc tactgcaatt gtcagttggt caagaaggtg
    92701 gaacatctct tttttgcttt ggagctgtgc tccaagaatc tttttttcag aatcaaatag
    92761 aaatttgagt ttaactttgc tttgatttat tgctgtaagc ttgtagtcta cgtatcctgt
    92821 ttcaattttt tggggataaa aaatgctctc ttcttctgtc aaacctacgc ttcctaagaa
    92881 atagccaaac cactgattgc cgactggacg ataggttccc tgatcctttg tctgtgagcc
    92941 actaattgtc ttgctgacat ttcgtgctgt acggacagcg ttatttacta gagaaacata
    93001 cattttttca tttgttggac gaaaagttac ttggatcgca tcgccaatcg cataaacgtc
    93061 tttttgagac gtttgcaagt agtcatctac ccagatcgtt ccatcatcat tcaatgttaa
    93121 ttgttcttcc cacatctgat tgtcgggacg agaatgatta gccagcaaaa caagatcata
    93181 gttctccttt cttttttccg tctctacaat agcggtttca ttttcggtca aagacacaac
    93241 ttgttcttct aaaaaaagtg aaagcccttg atttttcatt tcctttacta acggttctgt
    93301 catttcagga tctagataac gaaaaagaat tgactttcgg ctttcgtaaa gatggatttg
    93361 tttttttctt gatgccaacc cttcagctat ttcgattcct gcttgtcctg caccaataat
    93421 tgcaattttc tttgcattca tgatcttttg ctgaagagcc gttacgtttt gtatcttttt
    93481 aactgggtga ataagcgttg attctgcaga aatatttcga aacttttgat ttgaaccagt
    93541 cgctagaatc aaacgatcaa aatccaattt ttttcctcct gctaaaacga cttttttttc
    93601 tttcaatcct attactgtct tttctgtgta aacatggatt cgatagcggt cagtcagttc
    93661 ttctttcgtg atccaagaaa gttgtttttc actggtaacc tttcctttta atatcaaatt
    93721 gaacccgctg ggaatccatc ctaacttcga ttgcttttca atcaaatata tttcacttgt
    93781 tggcatcaat tggcgaagat aaatagccgc atgaatccct gcaaacgacc cgccgacaat
    93841 tacaatgttc atctgcattc ctctttctta taaggtgttc tactgctaac ttctcaatca
    93901 gaaaacacag tattcataac tcgctttcaa ccttatgata catagcgatg cagcagaatc
    93961 aaccctctcc atcataccgt gttctaggtc tgcttctcaa taatgtacta gtggacctaa
    94021 ttgaagagta tcatttctga agagatgttt caaaaaatgt actcttataa aagaaaaaaa
    94081 ctcgcctttt acgtacctct gtccaaatag gaaacagaaa agaacgaaaa gacgagtttt
    94141 agcgtgtgta atgtaatagt tagaatagac acatcattta tttttaattc tttaatgctt
    94201 caacaactgc ttcgcctaat tgatgagcag attgaggatt ttgccctgta agaagccgcc
    94261 catcaatttg aaccacttct gaaaaagctg ctccagactg atagtttgct cctttttctt
    94321 tcaaagcatc ttctgctaag aatggtactt cattcgttgt accatttgct tcctcttctt
    94381 cattactgaa cccagtcact gtacgtccag ctaaaataga ttttccagac tcatctgtaa
    94441 ctggcaacaa tcctacaaca ccgtgacaaa cagctgagac cagcccttca tttcgataga
    94501 tttgtgaagc gactttccca agatctattg cttctggaaa atcccacatt gttccgtggc
    94561 caccagcata ataaaccgct gcataatcat ttgcatccac atcatcagga cgtaaggtat
    94621 ttcccaaggc ccgatttctg aaatcttcat cttcataata ggcccaatcg atttcatcca
    94681 tattcaaaac actttgcggt tctaaaggta catatccgcc tttaggactg acaaaatctg
    94741 catcgatccc attttttact aatacatcat aaaaatgagt cagttcactt aaccacaaac
    94801 ctgtaggacg ctcgatcttt tcgtactttt ctacatttgt taatacgatc aatactttca
    94861 ttttcccatc ctctttctat aaaaattgcg ttattgataa cgtgctttca agaaattata
    94921 taatgggctt ggcaaataaa gctctttata tgcttcttca aaaggtttcg ttacaaattc
    94981 atcctcacgg ttttccaata atttctctgc ccagtcagga tcgacgatca tttgctgtcc
    95041 aattgctgca agttctgcat atttcaatac ctgctctaca tcttcgcgat tgcggatccc
    95101 accgacacca actaaaggga tctttccttg aatcgtgtta tggaccactt caagaatcga
    95161 tttatcgttg tatttatcag aacgagctat tcgatcatac atatttaatg aaacatgcag
    95221 ataatctagt tttgactcac gtaatcgttc aagaagccag atcgtgtctt caaaacggat
    95281 acctggtgtt tcatattctt ctggtgagaa acgataccct acgatgaatg gttttttagc
    95341 atattgttca accgctgaga aaaccgttgt taataattct tcaataaaac gataacgatt
    95401 ttccaagcta ccgccccatt gatcctctcg tctattcgaa tgcggagaga aaaattgttg
    95461 gatcaaatac gtatttgctc catgcaactc gattccatca aatcctgatt caatcgctct
    95521 acgtgtggct ttgccaaatg cttggattgt ttcaacgatt tcttcttcac tcattgctcg
    95581 tggtgtttcc gcatcaggac gttttgctgc aactgcactt gcagatacag gctgttctcc
    95641 agataaagtt gctcgacttg tcattcgtcc accgtggaaa atctgaataa tcgcttttgc
    95701 gccttctcct tggattgctt tcgctagctc acttaatcga ggaatcattt catcatgagc
    95761 gatgctgagt tcacctggcc agccttttcc gatatcttgg acatttgcag caccagtaat
    95821 aacagcacca agacctttag aacgttgttt ataataagtg atctcatctt cggtgatcac
    95881 tccattgaaa aagctgagtt gtgtcgtcat aggcgacagc atcaatcggt tcttcaattg
    95941 aactcctgaa ggtaaagtaa ttgattgatc aaattgtgac attgtcattc ctcccataat
    96001 tcgctctgat taacctatcg agaatcatct atcattaaac cactcgatta gttgtacatg
    96061 caactaattt gcttaaaaaa ttttattttt tttgtaactt atctgttgca tcattcattg
    96121 tctgggctaa ttctttactt gcttcatttc ccaaaactgc tttgtatgac tgatgaagtt
    96181 cttcccagct accatcgact tccttagcaa attggcgtcc ttgttctgta agataaacta
    96241 atgacaagcg accttcgcta actgttttga cgtatccttt atattctaat ttttcaacaa
    96301 aacgagcaat cgttgatggg gcggaataga gcatttgtgc aatatctttt tgtggtattc
    96361 catctttttc ttttaatatc aataaaataa aaccataagt tggtgccaga cctgtccttc
    96421 taaacgcttc atcagctaat ttattcaaca tgcgatctag ctttttcacg ctaaagaaaa
    96481 gacattcatc taacgctttt tcaattggct tttccatttc atcccctcca ttggcgtaca
    96541 tatcatagta tgatttttat tttttgtcaa aaaacacgga ataatgtccg cctcctctgt
    96601 ctagatttga aaaaaagaag tagccatctt caaagaattt cggcttgttt tcgacaaaac
    96661 tccttctgga acaccgtgaa tcagaggttg tgaaaaagtc gtttagtttt gagtatgaag
    96721 aaaaattttg tgaaaatggc tttccacatt tttgcataaa tttggcttaa taccgaaaaa
    96781 ctggcttttg aacaccgttt atatcagagg ttgtgaaaaa gccgtcctgc atcaagtaat
    96841 aagaacagcc aaacaaaaat agttcctcat attttgtcgt tttttgagct aatgccgatg
    96901 gtcagctgat tgtacaccgt ttattgagag gtcgtgagcc tgccgttgaa ctccgagtca
    96961 caaggagcaa tttcaaaaaa cagcttctca tgttttatga ctatggtgac ttgtgatcga
    97021 ggagttggca ggagaatact gtttattcgt aaagaagggg ctgtgacaag acttttgtca
    97081 cagtccctta tttcctttta ttataggatt tcgccatttg attggatgac ttgtctgtac
    97141 caatcaaatg attttttctt taaccgtttc tttgtgccat tcccttcgtc atcttgatca
    97201 acatagatat atccataacg ttttgacatt tgtgaagtag aagcgctgac gatatcaatg
    97261 cagccccacg acgtataacc aaataaatct actccatctt tgatagcttc cttcatctgc
    97321 tcaatatgct gcttcaaata gtcaatgcgg taatcgtctt gaacggttcc atcttcttct
    97381 actttgtcta tagctccgag tccgttttca gctatgaaca gtggtttttg atacctatca
    97441 tataattgat tcaaagcaat tctcaaaccc ttaggatcaa tctgccagcc ccattcactt
    97501 gatggaagat acggattttt aattcctcca gttattaagt ttccttggac cccttcccaa
    97561 ttttctggat gagcactgac tgcactagac atgtagtagc taaaagaaac aaaatcaaca
    97621 gggtaggctg ctaagatttc ttcatcacct acttctattt ttaagttgac acctttttct
    97681 tcaaacattc gttgcgtata cgctggataa tatcctcttg cttgaacatc actaaagaac
    97741 aaagaagttc tttgctgctc aattgttttt tgaatatcat ccggatcaca agaatacgga
    97801 acaagaagct gatagctgat catacaaccg acttgtgaac ctttgataat ttcatgacaa
    97861 gcttttgtag ctaaagcact tgctataaac tgatggtgtg caccttggta tttcgcttgt
    97921 tccaaatggg gatggttttc ttcaattact cctacgctaa caaaagggtg atgttttaca
    97981 cagttgatct cattgaaagt catccaatat tttactttat ttttatagcg tttgaatact
    98041 gtttcagcga aacgaacgta aaactgaata agctttcgat taggccatcc accatattct
    98101 gtaaccaaat ataacggcat ttcataatgg gaaagagaaa cgactggctc aatcccatat
    98161 tttagacatt cgtcaaacac ttgatcataa aaaaataatc ccgcttcatt gggtgtttct
    98221 tcatcaccat gaggaaaaat tcttgtccat gcgatagaca tccgatagca tttaaaaccc
    98281 atttcagcaa ataaacggat gtcttcttta aagtgatgat agaaatcatt tccgtgtctt
    98341 ttaggataat aatacttgct gttgggatcc tgcgccgctt ttacttgctg atgcgtcatc
    98401 atatgccatt gatctgtcca tttttcttta ggtaaatcgc tgtcgaatgt atacacatca
    98461 gacacagata atcctttttc atctacatcc catgcacctt cacattgatt ggccgctgtc
    98521 gctccgcccc ataaaaatcc ttctggaaat tttttcatat cgatccccct aaacttgcta
    98581 gttttcttgt ttgattattt ttcttatgac tagtaatgat atttttgaat ctactactat
    98641 ttgtaacaat aaccattgtg gtcggatcat atccctcttt gattaattct tgcaaatcta
    98701 cttccatcaa taaatcgcct ttacgtactt tttttccatc agatactgca ctttgaaagt
    98761 atttgccatt taaattcact gtatcaatac cgatatggat cagtatctct gctccttctt
    98821 cactgactac cccaattgca tgtttcgttg gaaaaactgt tgaaacttcc ccgtttacag
    98881 aactgtagat ttttccgtcc gttggaatga tcgcaaaccc ttctcctaaa gatccactag
    98941 caaatgttgg atcgtttact tcagataaat caattatttc acctgctgca ggagtactta
    99001 acactatatc ctttgaggat tcttcttcga tttcatcctt gtagccgaaa agaaaagtta
    99061 gcatacagga actgatcatc gctaagaaat aactagcaac tagccaaaca aatccttcac
    99121 caatataaat cgggaaagac aaaatacttc ttgtagcaac tgccaaagca ccggctcctg
    99181 cggcaccagc tactccacca aatatagctc cacatataca agcaatgtaa aatggtcgtt
    99241 tatatttaag agtcacacca taaacagcag gttcagtgat tccaaataat gctgtgataa
    99301 atgcagataa agataaactt tttactttct tatttttcgt tttcaaaaat accccaagta
    99361 ctgctcctgc ttgcgcagct acggcagtca tacatgctgg acctagcgta tcacgaccgt
    99421 agaaagaaag attgttggtc attactggta caagtcccca atgtaaccca aaaatcacta
    99481 acacttgcca aaatccgccg ataacagcac cagataaaac agggctaaag ttataaacag
    99541 ctaaatatcc agctgcaatc agctgacttg cataatctgc aattggacct acaactaata
    99601 aaataattgg gaccatcacg atgatacaaa tgaatggtgt caataaattt cgaatacttt
    99661 cgtgcaagct tttgcgaatc ttaggctcaa tataagaaag aacccataca gctaaaattg
    99721 ctggaataac cgtacttgta taccttgcta gaatcacggg catccctaga aatcgcaagg
    99781 ttatgctatc actatatgca ctgacaatcg taggataaac gagagctgct gcaataacca
    99841 tagcgatgaa acgatctgta ttgaactttt ttgcagcagt atatgccaaa aatataggca
    99901 agaaatagaa aaatgcatca gctgctgcat tcaaaatgat ataagttcct gattcagtag
    99961 ttaataaatt caaactcgta cataaggcga gtactccttt gatcaaacca cttcctgcca
   100021 agacgcctaa agtcggggca aaaacagcgg cgatcatgtc tataaaagag tttaaaatgc
   100081 cttttttctc agttcctctc tttccactag atgcatcatc ttctagtttc atttgtttgg
   100141 ataatacgtc atacacttca tttactgcat ttcctactac gacttgaaac attcctccag
   100201 cttcgactac agtgatcaca ccttctagtt gctctacctt ctccttatct gccttcttat
   100261 tatcttttag ggtaaagcgt aaccttgttg cacaatggta aacattgctg acattttctt
   100321 cgcctccgac aaatcggaca atatctttcg ctaattgtga atagtccatt tttacttccc
   100381 ccttgttaat taatgccgta gcaatccttc ttacaagata agtatagttc ttagaaaacg
   100441 gtttctccat tcaattagta ccccttctaa tagcgaacga aactgtacaa tagtgtgtat
   100501 ggtttactta gccgagcaaa tcactcactg cttgaaagaa aacaagtata tcgtttctct
   100561 tatagtcaaa ggagacaaaa aaaacacgct tgatcttcct ttgctgatgt ataagtggta
   100621 tctcaagctg atactctgtg atttttgttt ctacttggct tttgataaga cagcacaaca
   100681 aagtctcggc atttaacgga agcataacta ggtcagaact aggagagatt agttgctgaa
   100741 tggaatctat ctttttatgg atttttattt gattttcttc tttgtagtaa tgatcaagca
   100801 gttttgtttt ctttttttct tcaactatgg tcagcttttc acgcaaaatc tgataaatct
   100861 cccaagattc aaactcatct agaacactag gtagataaat caaatcatcc cactcaaaaa
   100921 taagctcttg ctcagtagtc aaaattgcat catattccgt tattttgttt gattgatttt
   100981 tgaaaagata cactggttct atccggcatt ctttgatcca catgttcata ctttgttgaa
   101041 tctgtttttt tagtgtattc aaaatactaa ttggctggtt actaacaatc agtatctccc
   101101 cttgttttcg aaaaggattc aacgcttcgg caagatacaa aacaaaataa gcgctttcag
   101161 ctaaattggg acgtaattga aaatgtattg ggaagaaacg ccgaattaaa taaatttcta
   101221 aaggataacg ggagatgatc ttttgtacaa aatgattcat ggtattatgt ccggcatcca
   101281 tcctcgtttt catttgtttg atatgggtta gcaaaaattc tgtttcatgg aacaataacg
   101341 aaacaggaag atctaaaaaa cgaataatcg tttgctcaaa tgctcttaga ttcttctcta
   101401 tttctttgtt tttttcatca ttaatcatca aataatttaa ctctaatagt cgattttcaa
   101461 ttgcttgttt ttcttgattg gaaaaagaat agggtagttc tgatgataac ttttttgtga
   101521 gggtctctac catcggaata aataatgtat acctgtttgt tttttctaaa agttcttctc
   101581 ttcgaagaat ggtcaaaacg agataccgag aaaatcgtat gaagtcctct cctgaaacaa
   101641 tataattata tgatataaga acctctttta atgtcttttg gacagtcggc atgatattgg
   101701 aaaaaattgt actgatttgt tgaggaaacc ttgcgtgttg gatcaccgtt tctgttccaa
   101761 caagagataa gaaaattctt tttgaatttt cttcagcaat gatttttagt ccttttgtcg
   101821 acgaaacttc cagttctaat ttaggattgc gctcgatcca acgcaaaatc gtttttattt
   101881 gtaatgatac ggctgttttt gaaagaaaga aaatgtctgc tatcttttgc atactgacat
   101941 accctttttg aaacaataag tacgtcacaa tagttgaagc tcggaaatcc atgctggaat
   102001 agattttatg tttcgtcacc tcttgtatga tttgctgttt agtttcagat tcaattgatt
   102061 ctaaaaaata acctgaacgt ttatttgact ggataatgat tttcttctcg tagatagcaa
   102121 gcatattttt gatttcagtt tggatcgttt tcttgttggt acctaaagct tcagctaatt
   102181 cttcgcttgt tatccaatga ttcttatttc ttaatactac aagaatttgg agttgtcgtt
   102241 tatttaagtc catttgctta cacctcctct ttgttataaa catacaggaa aaacctctca
   102301 aatgacaata gtcacttgca gaggtttaaa aatcatttga agaaagatct atattcaagg
   102361 tttttaccat tagactggat aatttgttta taatattcaa aactatcttt tttcaatctc
   102421 ctaaagcttc cgctcccatc attttgtcta tccacataaa taaagccata tcgcttcctc
   102481 atttcccctg ttcctgccga tacgatatcg attggtcccc accataagta accaatgatt
   102541 tggcaaccat cttttattgc ctcagcaatt tcctgaagat gtatttttag atactcacaa
   102601 cgttttggat cgtggattcg attcaattca tctggtgctt catctagtcc aatgccattt
   102661 tcaactatga ataatggtaa ttcataacgg tctgtcaatt cattcatgac ataacgaaac
   102721 cctagaggat caattggcca actccaaggt tcgggagatt tttccttcaa aaagggatta
   102781 tcgaaaccat ttgctccgcc agtatctacg cgcatttctg cttctttttt atagacagcg
   102841 ctacgataat aactaaatgc aatataatct gccgtgttag cttgcaagca ctgttcgtct
   102901 ccatctaaca ttactggtgc acagtcttct tccttccatg tcctgtaaat ataagctgga
   102961 taatgccctc ggcacatgac atcggagaaa aaccatgaat tgcgtttaaa ttgcagtgaa
   103021 ccaaatacat tctctggatc acagtcatat gcataagtcg ctaatgcaga aaagctacac
   103081 ataataccca tcttagcatt tggatcgatt ttctttaata attgagtagc acgggcgttc
   103141 gctagaaaca tatgatgaca ggcttgataa atcacttttt ttgttagtgt agcaatgggt
   103201 tcaagtttat ctctagcagg gaatgtaacc aatccagcag ctgcataagg catacgaaaa
   103261 gcattattga tctcattaaa ggtcatccaa tatttcactt tcccttggta gcgttcaaag
   103321 acggtctgcg cataaaattc aaaaaagtcg attaattttc gattttgcca ccccccatac
   103381 tctcctacca atgctaaggg ggtttcataa tgactaagag taattactgg ctcgatatca
   103441 tatcgattta atgtattgat tagtctgtca taaaatacga gaccagcctc atttggcttc
   103501 tgttcgtctc ctcttggaaa tattcgtgcc catgagatgc ttgttcgaaa agcttttaaa
   103561 cctaactcct tcagtaaacg aatatcctct tcaaatcgat gataaaaatc aatcgcttca
   103621 tgagaaagat aatggagtga ttcgtctaaa gcaatctccc aaatcttttt ttcttcattc
   103681 cactgaatac taggatgctg gttcatgatc ccgataagcg tatctgtcac attaggtaat
   103741 ttgccgtctt ccaaccatgc accttcacat tgattagcag ctactgctcc gccccataaa
   103801 aaattttctg gaaatatttc ctgatccata actatctctc ctcgctatca acaatttctt
   103861 acttgaagta tagctcgatt catcaaaaca agatattcta actatttcgg cagaagagaa
   103921 gaaaaaaatt gaatgctact cttcattttt aatatttata tataacaaat acataagacg
   103981 atccccgaaa gaaaacactc gttaaccatc taagcaaacg atttatttta cttccaacac
   104041 aaatgcttca taaggtcgta aatcaacttt gccgtctggt aactgtttca gaggataatt
   104101 tgacacaata acccttaaag cttcacgtgg aatagtgaac gtctgctttt cactggacat
   104161 attacaaata acgagccatt gctggtcttc atatatgcga gaataggcaa agacatgctc
   104221 aggcgtcttt tccactaatt catagtctcc ccagacgatg atgtcatgtt ttctacgcaa
   104281 atcgatcaat ttcttgtaca tataaaaaac agaatcttga tctgctaacg cttcttctgc
   104341 attgatttct ttataatttg gattgactcg aagccatgga attccagtag taaacccagc
   104401 atgttcacgg gcgttccatt gcataggcgt cctagcattg tctcttcctt tcgccttgat
   104461 tgattccaag atttcctctt ttgtaaatcc agaagaaatc ctttcgttat acatattgat
   104521 tgtctcaata tcttttgctt ccgcgatatc atcgattggg caattcgtca tgcctaactc
   104581 ttctccttga tagatataag gggtgccttt catcatatgg agcaagatag caaacatctt
   104641 ggcactttct tttcgataca catcgtcatt tccccaacga gaaacaattc gcggaagatc
   104701 gtgattattc caaaataaac tattccatcc ttcatcacct aaagatgttt gccatttgga
   104761 taatactttt ttcagttcag agatactcaa gggtttcaaa tcccatttgt ctttgccttc
   104821 ttgctgatct aatcctatgt gttcgaattg gaagaccatg gataattcat ttcttttagg
   104881 attggaataa agtttagcga tttcggtagt tgctccccat gtttcgccaa cagtcatcag
   104941 atttttgtca ccaaaagttg cttgattcat ttcctgaaga tactcatgca gtttaggtcc
   105001 attacttgtg atttctttat ccggttcttt gccaatcata tcgatcacgt ccatacgaaa
   105061 gcccccaata cctttatcga tccagaaatt catcatttga taaatctctt ggcgaacctg
   105121 tttatttttc caattcaaat caggttgacg cttgctatac aagtgcagaa aatattgccc
   105181 cgttttttca tccaactgcc aagcagaacc actgaatatt gagcgcaatc tattaggctc
   105241 ttttccattg accggatcac gccaaatata atagtcccga taaggatttt ctttattttt
   105301 acgggcctca atgaaccacg gatgttcatc agaagtatga ttcaccacca gatccatgat
   105361 gatacggata tttcttttct ttgcttcttc aatcagttct tccatttctt ccatcgtacc
   105421 gaattctgcc atgattgctt gataattact gatatcatac ccattgtcat cattcggaga
   105481 ctcgtatact ggagaaagcc aaattgcatc aatacctaaa tacgccagat aatccaacct
   105541 ggaaataatc ccccgcaaat ctccaatacc atcttgattt gaatcttgaa aacttcttgg
   105601 atagatttga taaacaactg aatggtgcca ccatttttgt tccatgattt tcctcctata
   105661 aatggatgtt tctatttttt gcttaatcat ttattttagt ctctctcact aaaaattgta
   105721 acgctttcaa atagctattg caccttgtta tcggtaacag aataaaacaa aaaaacagct
   105781 gaggaaattc cctcaactgt tttttgaaag gattgattta tgcagggaag aatgctgatt
   105841 tcacaacttc tttataaacg gtgttcactc agctgtccct tggtattagc tcaaaaaacg
   105901 gcaaaatata agaaactatt ttttcgtttt tttctctaat actcaggcca gaacgctgat
   105961 ttcacaacct ctttatttca cattcaatat ccacatgaat cccacaaata caaagaacag
   106021 accatacatg attggagata cttcttttcc tcttttagct gctaacatgg taattggata
   106081 gaagataaaa cctaatgcaa tcccatctga aatactataa gtcaaaggca tgccgatcaa
   106141 gatcaagaat gaagggatcg caatctctaa gtttccccat tcgatttgtc gcaaagattg
   106201 tgccatcaat actcctacca taatcaatgc tggtgcagtt acttgtgaag taacaactga
   106261 tagtaatgga gaaaagaaca agcctaacaa gaagaaaatc cctgtagtaa tagctgtgat
   106321 acctgaacgt cctccaacgg cgataccagc tgatgattct acaaatgcac caacaggaga
   106381 agtaccaaat aaggaacccg caagcatagc tgttgaatca gaagctaaag ctttccctac
   106441 acgaggcatc ttgttgtctt tcatgaaacc tgcttggttt gctaatccaa ctaaagttcc
   106501 tgctgtatca aagaaagtca caagcaagaa tgtcagcact acgacccaca tttgaagaga
   106561 attgatgtcc cctacatgtt ttaatgcaac taaaaatgtt ggtttcaaac ttggtgctgc
   106621 ggcaacaatt tcagaaggca ttggaatcaa acctgtgacc agacctaaaa tcgttgtagc
   106681 tgccatcccg atgaagatgc cacctggtac acgccgaaca agcaagatag cagttacgac
   106741 taatccaaaa atcgtcaacc aagtagaacc aacgttgagt gatcctaatc ctactaatgt
   106801 agattcattc gaaacgatga tcccaccttc gctcagacca aggaaagcga taaatagacc
   106861 gataccgcca gaaatcgcat atttcaaatc tgctgggatc gcatcaatga tcatttcccg
   106921 cagcttgaag attgtgatca aaataaaaat caaagaagca acgaatacgc cggctaatgc
   106981 tgtttcccat ggaatcccca ttcctaaaca aacagaataa gcaaaaaaag cattgatccc
   107041 aagagcaggt gccgttccaa ttggataacg ggctaatacc cccattaaaa cacagccgat
   107101 cgcactggct aatgcagtcg cagtaaacac tgctccttca tccattccag aagcacctaa
   107161 tacagttgga tttacaaata agatataagc cattgagata aatgttgtaa atcctgccag
   107221 tacctcacgt tttaccgtgg tgttcaattt ttctatttcg aagtaggaaa gtattttttc
   107281 cttcatcttc catcccccta aatccgaaca ttataaataa agtccgttaa aatttcgatt
   107341 tattaataaa acaacgtgtt ttatcatata ctaaaacaaa acataagtaa acaaaaaatt
   107401 cggattttaa gcaaaatccg aacgttccat ctgattaaat ccctttatct caaagttcat
   107461 gaacattcac tttggattac aagaatttct ttattgtgtt aataaagttt cttcattcta
   107521 gtctcaaaaa tcaatagtgt gatttgattt taccatttcg tttctgttct tctttgatga
   107581 attcttttgt atcgacagga ttcagattat ttagtccgcc aaaacctgta tcgaccattt
   107641 ttccccagcc ttttgtcttc ttttttcctg atttgagatt ctttgctcga agcttctcac
   107701 tatgttttgc tcgccgatac ttcaaataac cttctaatct tcccattaga tccactgttt
   107761 ttcctccttt ttattcaatc agacgactca gcttttccta taacaaaata attgccttca
   107821 gggtcagaaa aattgaaagt caagatgcca ttgttatctg tgatttctcc tggttcttcc
   107881 atgttctcgt agatagtttc aaattcatcc gtaaaaaata ctaaagaagg cggtggtcct
   107941 aatacctctg gtgaaaactt ctgcacaaat acttttggga atacgcctaa ttctaattct
   108001 tttgagatgc tgagtacaat actcttaaat tcttctggca attcgattgt ttcagaaatt
   108061 tctgcaccaa ttttttctac aaagaatcgg caaatcatct ctacgtcatt gacataaagc
   108121 atcacacgtg ttttttgtat catcatttga cccttctttc tatttgattt tctttcctgt
   108181 ctataaactg attccattat atcacgaaag cgcaaacacc ttgtgtttga tactattcaa
   108241 gaaaaataaa gcaatagttt ctaggattgc tttcctccac aaactttttt tacaaaaaaa
   108301 aaacggcaat gaatgccgct cttcttattt cgattctttt ataattctgc ttctgattca
   108361 tctttggtta gtgtgtgtct aacactagtt acgccaaatg tttgatcgat tttttccttc
   108421 aacgcttcga ttgcctgtgc gccacgtgcc gcttgttcag cattgatgga ttcaatgacc
   108481 agcaccgctg cttgtgtttc ttccgtcagc atcgttcttt gggcttcacg ccagttgaga
   108541 cagcgacaga tcgctccttg ttcatcatag tagatgattt cttccggaag tgccggagca
   108601 tcggaatctg cacctaaagg caggaatgat tcgccacctt gggcttttcc taaatgcaga
   108661 tttcctgcga tcttttcgat atcttctccg ccgcaaggta cgccaaaaga caaggaaata
   108721 ctattataga tatctacaag cggattgatt gggaaaaatt cccgttcttg atttgcacgt
   108781 ttcagtaaag cttcaataga agaacgtgca ccttttttag tcttgaatgt ggtaaatgcc
   108841 tgacgccact gagcaatgac ttcgttttgg ctgaatgttt cttctgtaag gtaatttctt
   108901 gcttcttttg ctgcctcatt caataattga tgatacgttt cttcactttc ttttgttacg
   108961 tggttatcga taccatttac ggataagatt tcgatggttg cttcaggaaa tacttcccag
   109021 aaattggtat ccactattac ttttttcatc tgttcacacc tcttgattag aataattgct
   109081 tgatgaatag atagtctgtt tggggatcat ctcccatata aaaaacatgt tcgctgtatt
   109141 tgataaatcc ttttttacga taaaaagcga gcgcaacatc gttgtgttcc caaacaccga
   109201 gccaaatggc tgttttttgc ttttcttttg ctttttcaat agctgtatct agtaaaaatg
   109261 taccgatacc ttgatgttta aatttttttc gtacatagat tcgctcgatt tctaaaaaat
   109321 gatcagccat ttcctcagat tgactcgttc ctgtatttag tttcaggtaa cccgcaggct
   109381 cttgatcgat actcactagg aaaaaatcgg tttctttatc ttttagctct tctgtaagtt
   109441 tttctagtgc ataagcttgt gtcaagtaat cagctaaatg ctccggtgaa ttatcttttc
   109501 caaatgtatc tgtaaatgtt tcgatactaa tttgttgtag taagtctaca tctgaaggcg
   109561 tcatttttcg aatttcgtat ttattcacgg cttcttcctt tcaatagatc cttttattgc
   109621 cttttttgac aaaattccaa tcttcgctaa cattcttgca tactttattt aaaagatatt
   109681 ccaattgttt tgcttcttcg tcagaaagac catttaatgc gactgcattg gagtagttgt
   109741 tttctcgaat gatgaaagga tagatttctg ctcctttttt agtcggatag agtttcttga
   109801 tttttttatt ttctttatct tccaaacgtt cgatcatccc attggattct aatttcttga
   109861 ttgctcgggc agtcgttgta cgatctactt tgatcatttc tgctagtttt tcttggatga
   109921 ttccaggatt ttcgcaaata cgtactaaat aaagatactg tcctcttgtt aaatcgactt
   109981 ctttgaattc gatattgcta atagaatcta gcgctcgggc aatcattccg atgtctcgaa
   110041 ggatttcagc catttcatcc cacctttatt ttgtttgtaa gactagttca cctctttttg
   110101 ttgcaaatgc aactattttc aataaaaaaa tcaagttctt ttttaataac tagtaaagta
   110161 tattcttttc ttcttttacg aaaaagctca atcacaatag atctggtttt tgttttctaa
   110221 aataagtaac tgtttcttcg ttgttcgctt ttctgatagc tttggcaact cttctatact
   110281 aaaaaatccc atgttggaag tttcattatt ctcaataaac ttggcctctt caccgtggat
   110341 cgcacaagca aaaacaagtt tataatactg aaaaagctgt ggaatatctt tttgtttatt
   110401 cgtatcaaaa actgctcgca actgagtagt ttctactgtt aatcctgttt cttcatagac
   110461 ttctttacgc acattttctt caggagacaa tcctatttca gcaaatccac caggaagtga
   110521 ccattctttc gtccgtttat cttcgattag taggaccttg ccatcttttt tgatataggc
   110581 acgaacatct acttttggtg ttggatgtcc ttctccaatc gttgtgagcg cttttatttc
   110641 ttcgaaagtt tcttttccta tttcactgat cagttccaat gaaatcgtcc ggatctcctc
   110701 aaaacgctct ttatcaaata catcttttcc ataatataga ccagcttgag aaagtgcgag
   110761 tagtcttttg tattgagcca acttcttgtt cattctcttc ccttcttttt tagcaaatgt
   110821 atgactatgt aatcaagtat agaagactgg tttaattacg gcaaataaat ataatgaaat
   110881 ctgcaataaa taaaagaatg ttaaaaaact cgagaacaaa agacattcga cttctctggg
   110941 gctgtgaata aatggcgttc aaaagcttgc atctgcggta ataagcccca ccaaacgaaa
   111001 aatatgacga gctattcttg tctggctgtt cttattaatt gaggcaggac agctttttca
   111061 caacctcccc actaaacggt gttctcctac caactcctcg gtcacaagtc acaatattca
   111121 tcaaacatga gaagctgttt ttttgaaatt gctccttgtg actcggagct gaacgacagg
   111181 ctcacgacct ctcggtaaac agcgttcaaa agccaatttt tcgatattaa gccatttctt
   111241 tgttacttat tgtcattgat ttctttttct aatttatcca atagtggagt gtaatccact
   111301 tgagtatagt ctgttacatc aaattcatat agtgttccta actggaattt gtcatatttt
   111361 cggtcggcga tgatcttttt gaatgcttct ttcgtaatat gattggtccc tttcgttcga
   111421 tcttccaata cgtcaccata gtgataatga tccatgatat agctcaaatg acgatctgat
   111481 tcacgatcac gtttgtaccg acgttgccat aacacttcaa agtctgctgt taggcgaatc
   111541 gtgatcacct gatatccgta tctatctgct aattcttgga gtcgtgcttt ttgcttatca
   111601 ctaaaaggat actcggacac aatcactttc tttcctgctt ccatatacaa atcaagtgct
   111661 ttgtaataga aaggataaac tttcgtcctt tcttgttccg cccgttcttc caaattgtta
   111721 aacccgatgg attctgcata gtattccttg atttcatcga gcgtgatggc atacatatct
   111781 ggaaatcgtt cccgtagttt attcataaga tatgtttttc ctgtaccagg actaccagcc
   111841 agaagaatga aatacttttt catctcattt atttcctaag ttaattggac tttccaagtt
   111901 cataaacagt ccaatcttat gcttgacaat ttcttttact gcttcatttg ctggaatcac
   111961 attatggcga agagacttgt tgatttctgt ttcactaaag gtttctttag ctactttcgt
   112021 ccagttgact aaaagctccg ttccaacatt gaatttgcgg atcccgttat tgatcaattc
   112081 aggataatct tcttctttca cacctgttcc tccatggata actaggggga catcaactac
   112141 tgcatcgatt tctttcagca agggaatgtt tacttctgtt tttgatttga attgtccatg
   112201 attcgtaccg atagcaatag ctaatgcatc cacgcctgta gcttctacaa atttcactgc
   112261 gtcttccggt cttgtatata ccttgtcatt ttcagcgaca tggattcctt cttctgtacc
   112321 gccgattgtt ccaagttctc cttctacgga aacaccgtgc gcatgggcat attctactac
   112381 tgcttttgtc ttcaggatat tttctttgaa aggcagatgg gacccatcat acatgacaga
   112441 agtatatccg ctgtcgattg cttctttgat atcatcaaag tctcgggcgt ggtctaagtg
   112501 caagactgca tcaaccattt cttcttctgc aatagctgtc attacattga ctaacacatg
   112561 catgccgata tatttagcgg tatcgacact tgtttgaata atgatcggcg aacccatttc
   112621 ttttgctgcg cctagcatag caggaagcat ttctaggtta tgcgtattaa aagctccaac
   112681 tgtaaaattc aactcttctg ctgtttttgt tacttctttc aatgttgtat acatctttta
   112741 ttgccacctt atctttttat ttgtttcctt tgatcgttac ttgttttgca atttgtcgca
   112801 tctcatccat tttgcccatg tagtaatcga tcccttgatc atcaccagca cgagcagcat
   112861 ctgcacgctt ctggttatag aggctcatca gttttttata cccatctccg atcgtctgtt
   112921 tattttccaa actcttttct aaatattgga tggcttgatc gttcacatac aattcagaaa
   112981 atttaagtcc aacttgttca taaagtttcg ctaatttatg cttatcttgg gtagtggaaa
   113041 tctcttgttt caattgtgta atctctgatt gaatcttttc ggtcgcttct ggcgataggg
   113101 ctttttcttc ttttgccaga acttttttcg gttctttttc ttttttctta aaaaatccaa
   113161 acatcatttt ccctactttc aattaaaatg ctttcaaaac tgggcttaag atccaggcat
   113221 ataagagtgc tgcggctact gtatctacat cggttgctgt agcattgttg aatcccatcg
   113281 aattaaagat cgttactagc aaagcgggca ataatgtaat aaagaaacca tgagcaatac
   113341 cgccaatgat cgctcctcgg cgtccgccaa ctgcgttgcc aaatatccct gctgtccctc
   113401 cggcaaagaa attggttagc ataccaggta aaatcatcgc taagccgaac atgggcaaag
   113461 taaacatggc aataatcgta ccaatcgttg tcgtaatgaa acctaaaatg acagcatttg
   113521 ggctataagg aaagaaaact ggacaatcta aggcagggat cgcatcagga actaatttca
   113581 tagctatccc tcgaaatgcc ggcacgattt cacctaacag tagacgtaca ccagctagca
   113641 atacatacac acccactacg aattggatgg cttgtaaaaa ggcaaacatg atatagtttt
   113701 gatctcctga taattcagaa gcgaatggtt cgcctgcaaa taaaacagtg atgatatata
   113761 gcgggaccat cacgaccatg acagacaaat aagtatcttg cagaaattcg aaatgtgtcg
   113821 gcaacttgat atcttcgatc gactttttat tttcgccttt ctcaccaaaa agcttagcaa
   113881 caccggcttc aaataagtac ccgatcgtac aaaaatgacc taaagcaata tcatttgatc
   113941 cagtgatttt acgaatgatc ggttgcgcta ctgccggcat agcaatggcg aaacaagctc
   114001 cgataaaacc accgaccaag atcaatacga tccctcgcag accggcaaaa tagccaaaga
   114061 ctgtcgtcat cgttgccatc cataaaattg cttgaccagt taaaaaaatg tacttccatt
   114121 ttgtgaaacg agccaaaata atattgaata caaaaatggc taagaacgtc agtgcaatat
   114181 ctctacctaa acccaaatca ttcattgctt gaccattgat agattcaata gaagggatga
   114241 tcccttgcat atgaaatcct tcagtaaaga ttttcccgaa ataagttaaa cttccaacga
   114301 tgatgctcga accggcactc aacacttgaa atcctaggag tgtctttaac gtgccagaga
   114361 tgacttgtcc tgctgatttc ttctgcagta gtagacccaa catagcaatc aatgcaatgg
   114421 tgatcgatgc ctgagtcaaa atattttcga tgataaaatt gataacgccc atgtt
//
LOCUS       DACDIE010000004        88317 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.004, whole genome shotgun sequence.
ACCESSION   DACDIE010000004 DACDIE010000000
VERSION     DACDIE010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 88317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 88317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..88317
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.004"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..590
                     /locus_tag="IV250_000343"
     CDS             <1..590
                     /locus_tag="IV250_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8767658.1"
                     /translation="FLDMNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYF
                     GNTMGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLI
                     VGIYLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMA
                     VKELMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
     gene            complement(830..913)
                     /locus_tag="IV250_000344"
     tRNA            complement(830..913)
                     /locus_tag="IV250_000344"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(877..879),aa:Leu,seq:gag)
     gene            1296..2513
                     /locus_tag="IV250_000345"
     CDS             1296..2513
                     /locus_tag="IV250_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP8767659.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            2697..3533
                     /locus_tag="IV250_000346"
     CDS             2697..3533
                     /locus_tag="IV250_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8767660.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            3609..4757
                     /gene="nagA"
                     /locus_tag="IV250_000347"
     CDS             3609..4757
                     /gene="nagA"
                     /locus_tag="IV250_000347"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAP8767661.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            4978..6036
                     /locus_tag="IV250_000348"
     CDS             4978..6036
                     /locus_tag="IV250_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAP8767662.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            6055..7074
                     /locus_tag="IV250_000349"
     CDS             6055..7074
                     /locus_tag="IV250_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HAP8767663.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(7176..7892)
                     /locus_tag="IV250_000350"
     CDS             complement(7176..7892)
                     /locus_tag="IV250_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP8767664.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(7861..8646)
                     /locus_tag="IV250_000351"
     CDS             complement(7861..8646)
                     /locus_tag="IV250_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP8767665.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            complement(8637..9386)
                     /locus_tag="IV250_000352"
     CDS             complement(8637..9386)
                     /locus_tag="IV250_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAP8767666.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            9684..10208
                     /locus_tag="IV250_000353"
     CDS             9684..10208
                     /locus_tag="IV250_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8767667.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            10224..11723
                     /locus_tag="IV250_000354"
     CDS             10224..11723
                     /locus_tag="IV250_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAP8767668.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(11811..12413)
                     /locus_tag="IV250_000355"
     CDS             complement(11811..12413)
                     /locus_tag="IV250_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP8767669.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            12517..13473
                     /locus_tag="IV250_000356"
     CDS             12517..13473
                     /locus_tag="IV250_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HAP8767670.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(13778..14734)
                     /locus_tag="IV250_000357"
     CDS             complement(13778..14734)
                     /locus_tag="IV250_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8767671.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            14908..15468
                     /locus_tag="IV250_000358"
     CDS             14908..15468
                     /locus_tag="IV250_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8767672.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            15461..16286
                     /locus_tag="IV250_000359"
                     /pseudo
     CDS             15461..16286
                     /locus_tag="IV250_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
     gene            16296..16913
                     /locus_tag="IV250_000360"
     CDS             16296..16913
                     /locus_tag="IV250_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAP8767673.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            16931..17614
                     /locus_tag="IV250_000361"
     CDS             16931..17614
                     /locus_tag="IV250_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAP8767674.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            18297..19580
                     /locus_tag="IV250_000362"
     CDS             18297..19580
                     /locus_tag="IV250_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8767675.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            19596..20483
                     /locus_tag="IV250_000363"
     CDS             19596..20483
                     /locus_tag="IV250_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8767676.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            20500..21336
                     /locus_tag="IV250_000364"
     CDS             20500..21336
                     /locus_tag="IV250_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8767677.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            21339..21962
                     /locus_tag="IV250_000365"
     CDS             21339..21962
                     /locus_tag="IV250_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8767678.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            21959..23251
                     /locus_tag="IV250_000366"
     CDS             21959..23251
                     /locus_tag="IV250_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8767679.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      23610..23707
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            23823..24404
                     /locus_tag="IV250_000367"
     CDS             23823..24404
                     /locus_tag="IV250_000367"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAP8767680.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            24407..25714
                     /locus_tag="IV250_000368"
     CDS             24407..25714
                     /locus_tag="IV250_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAP8767681.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            26130..26618
                     /gene="purE"
                     /locus_tag="IV250_000369"
     CDS             26130..26618
                     /gene="purE"
                     /locus_tag="IV250_000369"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAP8767682.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            26602..27735
                     /gene="purK"
                     /locus_tag="IV250_000370"
     CDS             26602..27735
                     /gene="purK"
                     /locus_tag="IV250_000370"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAP8767683.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            27748..29043
                     /gene="purB"
                     /locus_tag="IV250_000371"
     CDS             27748..29043
                     /gene="purB"
                     /locus_tag="IV250_000371"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAP8767684.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(29223..29600)
                     /locus_tag="IV250_000372"
     CDS             complement(29223..29600)
                     /locus_tag="IV250_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8767685.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            29856..30584
                     /locus_tag="IV250_000373"
     CDS             29856..30584
                     /locus_tag="IV250_000373"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAP8767686.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            30584..30838
                     /gene="purS"
                     /locus_tag="IV250_000374"
     CDS             30584..30838
                     /gene="purS"
                     /locus_tag="IV250_000374"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HAP8767687.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            30840..31511
                     /gene="purQ"
                     /locus_tag="IV250_000375"
     CDS             30840..31511
                     /gene="purQ"
                     /locus_tag="IV250_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HAP8767688.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            31512..33734
                     /gene="purL"
                     /locus_tag="IV250_000376"
     CDS             31512..33734
                     /gene="purL"
                     /locus_tag="IV250_000376"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAP8767689.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            33719..35158
                     /locus_tag="IV250_000377"
     CDS             33719..35158
                     /locus_tag="IV250_000377"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAP8767690.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            35190..36233
                     /locus_tag="IV250_000378"
     CDS             35190..36233
                     /locus_tag="IV250_000378"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAP8767691.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            36230..36808
                     /gene="purN"
                     /locus_tag="IV250_000379"
     CDS             36230..36808
                     /gene="purN"
                     /locus_tag="IV250_000379"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAP8767692.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            36805..38340
                     /gene="purH"
                     /locus_tag="IV250_000380"
     CDS             36805..38340
                     /gene="purH"
                     /locus_tag="IV250_000380"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAP8767693.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALVFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            38356..39630
                     /gene="purD"
                     /locus_tag="IV250_000381"
     CDS             38356..39630
                     /gene="purD"
                     /locus_tag="IV250_000381"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAP8767694.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            39752..40666
                     /locus_tag="IV250_000382"
     CDS             39752..40666
                     /locus_tag="IV250_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAP8767695.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(40799..42364)
                     /locus_tag="IV250_000383"
     CDS             complement(40799..42364)
                     /locus_tag="IV250_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP8767696.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(42357..43808)
                     /locus_tag="IV250_000384"
     CDS             complement(42357..43808)
                     /locus_tag="IV250_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP8767697.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIRESKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(43822..44838)
                     /locus_tag="IV250_000385"
     CDS             complement(43822..44838)
                     /locus_tag="IV250_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8767698.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            45058..46146
                     /locus_tag="IV250_000386"
     CDS             45058..46146
                     /locus_tag="IV250_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP8767699.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            46200..47726
                     /locus_tag="IV250_000387"
     CDS             46200..47726
                     /locus_tag="IV250_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAP8767700.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            47728..48933
                     /locus_tag="IV250_000388"
     CDS             47728..48933
                     /locus_tag="IV250_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8767701.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            49190..50167
                     /gene="guaC"
                     /locus_tag="IV250_000389"
     CDS             49190..50167
                     /gene="guaC"
                     /locus_tag="IV250_000389"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAP8767702.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            50431..51855
                     /locus_tag="IV250_000390"
     CDS             50431..51855
                     /locus_tag="IV250_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP8767703.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            52316..53425
                     /gene="pdhA"
                     /locus_tag="IV250_000391"
     CDS             52316..53425
                     /gene="pdhA"
                     /locus_tag="IV250_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAP8767704.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            53428..54405
                     /locus_tag="IV250_000392"
     CDS             53428..54405
                     /locus_tag="IV250_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAP8767705.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            54430..56073
                     /locus_tag="IV250_000393"
     CDS             54430..56073
                     /locus_tag="IV250_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005236379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HAP8767706.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            56081..57487
                     /gene="lpdA"
                     /locus_tag="IV250_000394"
     CDS             56081..57487
                     /gene="lpdA"
                     /locus_tag="IV250_000394"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAP8767707.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            57649..57924
                     /locus_tag="IV250_000395"
     CDS             57649..57924
                     /locus_tag="IV250_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HAP8767708.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            57930..58697
                     /locus_tag="IV250_000396"
     CDS             57930..58697
                     /locus_tag="IV250_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HAP8767709.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            58865..60694
                     /gene="typA"
                     /locus_tag="IV250_000397"
     CDS             58865..60694
                     /gene="typA"
                     /locus_tag="IV250_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAP8767710.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            60955..62727
                     /gene="recQ"
                     /locus_tag="IV250_000398"
     CDS             60955..62727
                     /gene="recQ"
                     /locus_tag="IV250_000398"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAP8767711.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            62841..63143
                     /locus_tag="IV250_000399"
     CDS             62841..63143
                     /locus_tag="IV250_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HAP8767712.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            63146..64309
                     /locus_tag="IV250_000400"
     CDS             63146..64309
                     /locus_tag="IV250_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP8767713.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            64335..67763
                     /locus_tag="IV250_000401"
     CDS             64335..67763
                     /locus_tag="IV250_000401"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAP8767714.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            67784..68908
                     /locus_tag="IV250_000402"
     CDS             67784..68908
                     /locus_tag="IV250_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8767715.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            68898..69335
                     /locus_tag="IV250_000403"
     CDS             68898..69335
                     /locus_tag="IV250_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP8767716.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            69319..69657
                     /locus_tag="IV250_000404"
     CDS             69319..69657
                     /locus_tag="IV250_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HAP8767717.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            69776..70336
                     /gene="rsmD"
                     /locus_tag="IV250_000405"
     CDS             69776..70336
                     /gene="rsmD"
                     /locus_tag="IV250_000405"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAP8767718.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            70333..70824
                     /gene="coaD"
                     /locus_tag="IV250_000406"
     CDS             70333..70824
                     /gene="coaD"
                     /locus_tag="IV250_000406"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAP8767719.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            70814..71857
                     /locus_tag="IV250_000407"
     CDS             70814..71857
                     /locus_tag="IV250_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP8767720.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            72016..72744
                     /locus_tag="IV250_000408"
     CDS             72016..72744
                     /locus_tag="IV250_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAP8767721.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            72860..73105
                     /locus_tag="IV250_000409"
     CDS             72860..73105
                     /locus_tag="IV250_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP8767722.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            73102..73320
                     /locus_tag="IV250_000410"
     CDS             73102..73320
                     /locus_tag="IV250_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAP8767723.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            73456..73995
                     /locus_tag="IV250_000411"
     CDS             73456..73995
                     /locus_tag="IV250_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HAP8767724.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            73988..76261
                     /locus_tag="IV250_000412"
     CDS             73988..76261
                     /locus_tag="IV250_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAP8767725.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            76299..77345
                     /gene="holA"
                     /locus_tag="IV250_000413"
     CDS             76299..77345
                     /gene="holA"
                     /locus_tag="IV250_000413"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAP8767726.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(77434..77688)
                     /locus_tag="IV250_000414"
     CDS             complement(77434..77688)
                     /locus_tag="IV250_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAP8767727.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(78088..78357)
                     /locus_tag="IV250_000415"
     CDS             complement(78088..78357)
                     /locus_tag="IV250_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8767728.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(78827..79282)
                     /locus_tag="IV250_000416"
     CDS             complement(78827..79282)
                     /locus_tag="IV250_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAP8767729.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(79420..81348)
                     /locus_tag="IV250_000417"
     CDS             complement(79420..81348)
                     /locus_tag="IV250_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP8767730.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(81545..82378)
                     /locus_tag="IV250_000418"
     CDS             complement(81545..82378)
                     /locus_tag="IV250_000418"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAP8767731.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(82570..83274)
                     /locus_tag="IV250_000419"
     CDS             complement(82570..83274)
                     /locus_tag="IV250_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP8767732.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(83297..83923)
                     /locus_tag="IV250_000420"
     CDS             complement(83297..83923)
                     /locus_tag="IV250_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP8767733.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            84249..86624
                     /locus_tag="IV250_000421"
     CDS             84249..86624
                     /locus_tag="IV250_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAP8767734.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(86648..87139)
                     /locus_tag="IV250_000422"
     CDS             complement(86648..87139)
                     /locus_tag="IV250_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAP8767735.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            87326..88246
                     /locus_tag="IV250_000423"
     CDS             87326..88246
                     /locus_tag="IV250_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8767736.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
BASE COUNT        28619 a        15250 c        18966 g        25482 t
ORIGIN      
        1 tttttttaga catgaatatc ttatttttat tgctattttt cttaattttc cttctaacat
       61 ataggaaatg tcaaaactat tatgtgcgta tattgactgc attaccgttt ttcttaaacc
      121 tgattattta ttttggtaat acaatgggtc aaagctttac ttacgtcaat ggaaataaaa
      181 gggcaatgat ttggagtagt agtaacctga ataatctctt tacagagtta ggaacgaaac
      241 tgtcactttt ttatccaggt acttggatag ctactttagt agtcttggca ttattactat
      301 gtttaattgt tggaatttat ttaagttttg acaataagaa aacttctatt tttttagtta
      361 ttttgatgat aatgggattt tgttcaaggt taattatggg attttctccc acagtatggg
      421 cttccggaat gcggacgtac tatatacttt atgttgttat cgccatctta gtactaatgg
      481 cagttaaaga gctaatgaaa agcatgagtg tacaaaaaaa tgaatttatg caatttggat
      541 taaccgtgct tggtatttgt acctttataa taacggtgat aaacagataa tatacaaaaa
      601 atccctcgct ttagtattag gtgagggatt ttactcgttt ttcgattaat tgttttaaca
      661 cttgcaagtt tctacgagta gcttgtttcc gaccttcata tactcacctc aggctgttta
      721 catgattgta cgttcttttt gtaaaggata cagtgatatg tatataatgg cataaagaaa
      781 cgttcaaaca aaaaaactcg tgaagcaaaa ttaacttcac gagttgtagt gcagatgatg
      841 ggactcgaac ccacacgagc taacgctcac atgaccctca atcatgcttg tctgccaatt
      901 ccaacacatc tgcgtaataa taacacacta atgatagcga ggttttacat agttgtcaag
      961 ctcaaaaatg ggaggactta ttaagatgga gaaatatgac ggagaatttt caggactggg
     1021 aatgatctta ggtgtattga ttggacttgc attcggacgt tttttgcttg gttttatgct
     1081 aggaatcatt tgtggaattg caatggattg ggcagcgaat ctatggaatg actatcatga
     1141 caattaagtt ctcaacgctt gtttgtgttt ttttaatact tacgtttttc ctaaataagt
     1201 ggcaaaattt tctaggatgg agtatgatat tgaaatagta aataacaaaa aatttggaaa
     1261 gttctaactt tttgaaatga gggattggct aagtgatgag agaatttgaa aaatccaaca
     1321 aattagaagg tgtcagttac gatgttcgcg gtcccgttct agaagaagcg gagcggatgc
     1381 aagaagaggg cattagtatt ttaaaattga acacgggaaa tcccgcacct tttggatttg
     1441 aagcaccaaa cgaagtcatt cgtgatttga tcatgaatgc ccgtgactca gaaggctact
     1501 cagattccaa ggggatcttt tctgctagaa aagcaattga gcaatattgc caactgaaac
     1561 attttccgaa tgtgacgatc aatgatatct acaccggcaa cggagtgagc gaattgatca
     1621 cgatgtgtat gcaggggttg ctggacaatg gagatgaagt actggttcca atgccggact
     1681 atccgttgtg gacggcttct attgcattag ctggaggcac acctgttcat tatatttgcg
     1741 atgaagaggc agaatggtat cctgatattg atgatatcaa atcaaaaata acgagtcgga
     1801 caaaagctat tgttatcatt aatcctaata atcctactgg ggcactttat ccaaaagaat
     1861 tattagaaga aatcgttgaa gtagctcgac aaaataacct gattatttat tccgacgaaa
     1921 tctatgatcg cttagtgatg gatggtctag aacatattcc gattgctaca ttagcgcctg
     1981 atttgttcgt tgttacgctg aacggtctat ccaaatctca tcgtgtagca ggatttcgag
     2041 taggctggat ggtcctaagt ggagataagt cacacgtcaa aggctatata gaagggctga
     2101 atatgttgtc ctccatgcgt ttgtgttcca atgtattgtc tcagcaaatt atccaaaccg
     2161 cgctaggcgg ttatcaaagt gtcgatgagc tgctcctgcc aggcggaagg atctatgaac
     2221 aaagagaata tatctataat gcaatcaatg atattcctgg tctttcagcc gtaaaaccaa
     2281 aagctgcttt ttatatattc ccaaaaattg atacaaagcg attcaatatt ttggatgatg
     2341 aaaaattcgt tttggacttt ttacatgaac atcatattct acttgtacat ggtggtggat
     2401 ttaattggaa ccagccagat cattttcgaa tcgtttatct tcctaaaatg gatgatttga
     2461 aacaaacagc taagaaaatg agagaattcc tagcaaccta tcgccaaaaa tgattttaaa
     2521 aaccaaattt ttcagtagga gggccacaga caaacacact gaggctctct ttctagtgga
     2581 gttttcttaa ataactaaag tatattattt agttgcaagt cttttcttga agtttgcgac
     2641 ttcctaaagt aaaatacgaa tagaatgcga atacgcatat tttggggagg atacttatgg
     2701 aaacgttaat cgagtccttt ggaacgatca ttaaagagat tcgaaaagaa caaaaaatga
     2761 cgcaaaaaat gctttcgcat gatatttgtt cacaaagtgt attaagtcgg atcgaaaata
     2821 atgaggaact gccgaatgta ttggtgatga tgcagatttg tcaacggcta ggggtaacga
     2881 ttgatcatgt gatgaatctc tctcgaaaaa ctactcggac aaacgataaa ttatttgaac
     2941 tgttggatag ttgtttccaa aaacgtgatt atcggaagtt agaacaagta ctaaaatctt
     3001 ctgatatttc tgaaaatctg tatcaagcaa ctgactttca acgctattat tattatctcg
     3061 gtgtttgcga attcacgttg cgtcaaaata tctctcagtc cctttattat ttgaaagaag
     3121 cattaagcta ctctagccaa aaagaaagaa tccatgtttc tgacacagaa atacaactga
     3181 ttagttgtat cggaaaaata tatggtgtgg caggaaaaac ggctgaagca cgctactatt
     3241 tgagtcgaag catccaactg ctgcatcaat cagctgatga acgatcaaaa agtgaatttt
     3301 cgcaaatttt ttataattat ggaaatttct tattccatca aaatgaaatc gacgaagcgt
     3361 tagaacaggt gaatcaagga atctattggg ctcaagagaa gaatagctac tattacctca
     3421 atgatttatt tgtgcttaaa agcttaatat acaagcgtaa agaagaacca gaaagagcag
     3481 cattctatga aggcttagca caagctgtga aacaaatttc aaaaaatttg taaatggtgt
     3541 agaccattct tgacttcttc tctaaataag agtactttag aggtagaaaa acaaagggag
     3601 agaatagaat gagaactttt atctatgctg acaaattctt tttagcgtca ggtgtcaaac
     3661 atgcaggcta tcttgaaatt accgatggac tttttggtga atacaccaaa gaactacctg
     3721 agggagaagt caacatcatt gaccaaagcg gcaaatggat tgctcctggt ttggtagata
     3781 cacatattca cggctatatg aatcatgatg taatggataa tgatgcagaa ggcatcaaag
     3841 tgatgtctga aggtctgtta tcttgtggag tgacttcatt tttaccaaca acattgacgt
     3901 caagcaaaga acgtttaaaa gatgtagctg agacaatcgg caaaatgtat caggaagcac
     3961 caggagctaa aatcaaagga atctattttg aaggtccttt cttcacagaa gaacacaaag
     4021 gggcacaaaa cccaagttat ttcggggatc ctgatttgga tactttccat gaatggcaag
     4081 aagcttcagg tggtttaatc aaaaaaattg ctttagcacc ggaacgtcaa ggtgtagaag
     4141 agtttgtcaa acaagtgact gaagaaggag tagttgtttc tctaggacac agtaatgcta
     4201 cactagaaga agcgcaaaaa gctgttgaag caggcgcaag tgtatttgtc catgctttca
     4261 atggtatgcg tggattgaat catcgcgaac caggtatggt gggtgcgtta ttatctttag
     4321 aacatgtctt ttctgaattg atttgtgatg gccatcatgt acatccaaat gctgcgggaa
     4381 tcctaatgga aaaagcagga catgatcatg tcgcattaat tacagactgt atgatggccg
     4441 gtgggatgcc tgatggaaat tacaatttag gcgaatttcc agtagtagta aaagaaggaa
     4501 cagcacgtct agaatctggc aacttagctg gaagtatatt gaaattgaaa gaagcaatta
     4561 aaaatgttgt agactgggga ttagcaacac cagaacaagc agtcatgatg gctagtctga
     4621 ttccagcaat cagctgtaaa atcgatgata catgtggaaa aatcgctcaa ggacgcgatg
     4681 cagactttat cgtattgaac cctgatatga cattggaagc aacttatcta gatggcgtgg
     4741 aacgtttcca cgcataatat aatttaagtt aaccttaaaa ctaaacttct taagtttggt
     4801 tttaaggttt tttttgtgtt ctagtaaaaa tcactttaca taagtttcaa aagtttcttt
     4861 aatttcctat tgttcaataa gtaacaatgt aaagataaaa ttaaaaatgt taatacgtat
     4921 tagcaaaata aaagcttttt ttattgtgaa taaatgaaca taattaggag gagaaagatg
     4981 aacttatttg acatttttcg atcgactttg acagatatgt cgattgtcag tgcaatcact
     5041 tcaacagttt ttattatatt attagggttt gtttgtagaa aaaaggggat tttttctgaa
     5101 tctgttggaa aaattctatc gaaagtcgtc ttaacagtgg ctttgccagc actggcattt
     5161 cgagctttca tgcaagatat caatacagaa ttactgatgc aaggaatcaa tgtcttgatt
     5221 tggggattag ttatttatat tattcttatt ctagtttcaa aacctttttt cttaagctat
     5281 aaaggggata agcaaacaac gttgcgtgtc ctgacgatct ttggttctac gacctttttc
     5341 ggaacgccta tcgtcagtgc gatttatggt cctgtcggcg tgatgtattc ctcaattttc
     5401 aatattggct accgaatctt tttgtattct tatggataca taaaaatgag tggcttgaag
     5461 atggaagcta aaaatatcaa agcgatgttc gtcaatccaa tcattatcgc tacttttgcc
     5521 ggccttttca tttggctgtt ccaagggtat ctgccacaag tgtcagttac aggtgcagaa
     5581 ggagagataa gccaagtagc gtttttgcga attgaccaga ctgcaccgtg gctatttcag
     5641 ccgatggatt atttagctgg tttaagttca ccattggcgt ggctagcaat cggcgcaaca
     5701 ttaggtgaag taagcttcaa aaaagctgca tctgaaaaaa cttcatggta ttacagtcta
     5761 gtcaaagtgg tatttgtacc tgcattgaat atcgtgattc tagcagtttt gacggcaacg
     5821 aatattctac cagtcaattt tgaagcactg gcaacaatcg ttatcatgat ggctacacca
     5881 gctgcaacag tggcagcagc ctatgctatc agttttgata gggaggcact tttagcctcc
     5941 aatgcatcct tactgtcaac tatttttgct gtggtactga cacctgtatg gatcgttgta
     6001 ttacaaatga ttcatggcat aggtttattt tcataagaaa tggaggaaga aagtatgaga
     6061 atcgcatgtt acggtgttcg tccgaacgag atagattttt ttgaaaaatt gaatatttac
     6121 cattatgatc tcagtcttta tgaagaactg ttgacacatg acaatatcgt gtcagcaaag
     6181 gaccatgatg ctgtattgtt gaggggaaat tgtatagcag atgagaccaa tcttgcaaaa
     6241 atgcaggaat atgggattcg ttatgttttt acacggactg tgggagtcaa tcatatcgat
     6301 ctacaagcgg cagctgattt tggtatgaca gtagcccgtg taccatcgta ttcgccaaat
     6361 gcgatagcag agttatcttt gacttttgcc atgatgcttt tgagaaatac tgcgtatacg
     6421 acgatccgaa catctttcaa agattttcga gtggatgagc aaatgtttag tcgtgaaatc
     6481 agaaattgca cagtagggat cataggaaca ggcaggatag ggttgacgga agctgttttg
     6541 tttaagggac taggagccaa agtgatcggc tacgatattc atcagacaga agctgctaaa
     6601 aaagtattga cgttccactc gcttgaagag ttattagcca aaagcgatat tgtcagtatt
     6661 catgtacctc atatcccagg tgaaaacgat caaatgatca atgaaggatt tttagctcaa
     6721 atgaaaaaag gtagcatctt gattaataca gcccgtggag agcttcaaga taatgaagct
     6781 attttaagag ctttgaaaag caatcattta gctggttttg caaccgatgt ttttgcaaat
     6841 gaaacagagg tttttttccg atccttcaag ccatgggaga cgattccaga ccctgctatc
     6901 cagcaattag tagaaatgta ccctagagtt cttgtaaccc cgcatgtagg ttcaaataca
     6961 gatgaggcat tatcaaatat gatttcgaca agtttcgaga actttcgtga aataatagaa
     7021 actggaaaaa cgaaaaatga ggtatctctt cctaaagccc gtcaattaag ataagaaaac
     7081 atggatattc gtcaataaaa tgtttaaaaa tagtccaagt aaacagttgt tacttctgta
     7141 tacttggact attttgtatg ggataattcg atgactcaac agtaatctgt gagctttttc
     7201 cgcccattct ttgtcaattc ataacaggtt agcggccggc cgatttccaa atatttgatt
     7261 ctttcgttca atatatcttg ttctactaaa aaattcaaat atttcttgac ggatatacgt
     7321 gacagtgagc ttttttgagc cagttctttt gttgaaaaaa tagtatcttc ttttagaata
     7381 aatgcactta ttttttttaa agtcagtttt gacagacctt taggcaaatc gatgaatgat
     7441 ggtatttcgt gaatgggagt ggtgtggttg aatacttgat cgatcaattt ctgatcggct
     7501 ttttcagtcg tttggattgt ctgagagaaa gcaagatact tttcgatcgc cagctgaaaa
     7561 cgttgcaaag tgaacggttt gatcaaataa tctaaggcac cgtagcggat cacattcttt
     7621 atggtttcga tatcatctgc agcagtgatc aaaatcactg ggatatcatt tccttgctgt
     7681 ctaagccaag ataaaaaatg gataccactt tcgccaggca tgtatacatc aagcagcagc
     7741 agatctgcgt ttttttgtct gaaatatttt tttccttctt ctacatttct gaaactttcg
     7801 attacgttca tattagggat gctttccaca aaagtacgat tcaattctgc aaccattgga
     7861 tcatcttcga ttatcaggac atttgtcatc attttgatct tccttttttg gtaattctag
     7921 atagacgatt gtaccgccat ttgtttgttt ggaaaattct aaaaatccac cgtggcgttt
     7981 gacgattgaa tggatgagat tcaatccaaa cccacgattg attcccttcg tcgaaaaacc
     8041 tagcgtaaat attttttctg agatttcttc tgatatcccg acaccgtcat cgcagacggt
     8101 cacgatgaat acttcagctt ctgtgtcata gtttaaatga atccggacct gtccgtttct
     8161 ttcatgtttt tgtagcacgg cttcttttgc attatcaagt aagttcccta acacttgcaa
     8221 aagtgaatgg atggagtcat ccatctccat tttagggata aaagaatcgc tttctaacag
     8281 catttcaaca gattgttctt tggcttcgtt tattttgcct aataaaaaac cggcaattgc
     8341 aggtactttg atcagacttg agacttggcc aatctctttt ttataatttt gatttagctg
     8401 ctggataaaa tgggtgactt cttcatactt cttcaaatca atcaaaccag aaatgacatg
     8461 aagtttattc atgaattcat gggtttgagc tctcatggag tttatatatt gtttcgtccc
     8521 cccaagctcc tccattaatt tcattacttc tgatcgattt cgtaaagttg cgactgctcc
     8581 taaaaaacgg tcattgctaa aaagaggtgt aacgctagaa atagtttcta tcccatttaa
     8641 atacatggat tgagtccgaa cggtcgtttg atgctcaatc gtctctttaa acaaaacgtc
     8701 atatagatta tcactgattt ttttcctact tcgaaatcca atagctgctc tgtttggtag
     8761 aaaatgtttt ttgcaggtgt attgatcaag aggatagttt tatcagaaga gatggctaag
     8821 attccttcat ctacttcatt ttcgataatc gttttttcac ttaactgttc tgctatttct
     8881 tgtggttcaa gtccaagcag tacttttttc attttttggg caagaaaaac ggcgcctatg
     8941 atgcctacgc taagtccaat aatcaatgaa aaaacaatca ttttttgtcc atctttgact
     9001 tgattgtcaa tcgtgttgtt tgttaagcca acacaaactg caccaataat ctcttgatca
     9061 taataaatag ggacgaaata gcggaagccg aaccctaaca ttccttcttt ttcagagaaa
     9121 aattcttgtc ctccaatcac ttttttcaca tcttcatgac ttgagaaagg aagaccgatg
     9181 taacttgggt ctgcgtgaga atatcgaatg ccttcagggt ccatgacaac gataaaatct
     9241 aaattcgatg caatcatgat ttcatttgtt attccttgaa tcgcatgtgt cgggtcacca
     9301 gtctgcagct ttttttggat gagtttatca ttggcaacga ttttagcaat gctagagatt
     9361 ttatctgaca cactagatcg gttcatcgaa agaataaaat tcctgatcaa tatggcagaa
     9421 acaatcaaag aaacgaatgt ggagaaacaa acgacaagga tgatcgtgtt ctggagcttt
     9481 aatggttttt tcttaaaaaa aggaaacata ggctacctcg aaagaattta ttgtgaaaat
     9541 tttaacatga aaacaaaaat ttaacaaatt gtcaacgata aacacaacct tcctataaaa
     9601 aacaccactg agtcggtaaa agaagctata cagaattttc aaacattggt atgatagacg
     9661 tagaaatgga aggaggcgtg tatatgatag ggggaatcgc acttgagacg atttattggt
     9721 atacattgct gatttgtgca ggattggcag tcctattgat tatttttgga gatgtattcg
     9781 attttgatgg cccgcttgat ccgatgctta ttatcccttg gttaactttc acaagcttat
     9841 ttggttttct aggtgagcgt ttaacagatc aatcatcttt atttttattt attgctagtg
     9901 gaggcatatc gatagtgttg gtatttttgc ttaatttcta tatattgatg ccgatgaaac
     9961 atgccgaatc aacgatgtcg tcttctgaaa aaactctgga aggacaagta gctgttgtga
    10021 tcacaccgat ccctgtacga ggtatgggag aaattcagtt gaaaagtgtt accggttcca
    10081 ttagtcgtcc agcttgtttt tacagccccc aagaacagac agctgagcgg ggaacgaaag
    10141 tattgatcat tgagatcaag gaaagaatct gttatgtcat gccttatgaa agcatgataa
    10201 aagcttaaag gagcgtgtgt taaatggaaa taccagttgt ttttatcgcg atcgcaatcg
    10261 cagctttgat tttactgatg ttgctgatcg tctttgtagc gaagtaccag acagcaaaac
    10321 cggatgaagc gttgatcatc agtggaagtt atttaggaag caaaaatgtg cacattgatg
    10381 aaagcaataa caaaatcaaa attgtgcgtg gaggtggcgc attcgtcttg ccagtttttc
    10441 aacgttcgaa tcggatcagt ctattatcga gtaaattgga cgtctccacc cctgaagtgt
    10501 atacggaaca aggcgtaccg gtgatgtgtg atggtacttc gatcatcaaa atcggttcat
    10561 ctgtagaaga gattgctaca gcagcagaac agtttttagg caagacaaga gaagaactgg
    10621 aaaatgaagc aagagaagta ttagaaggac acttgcgctc gattcttggt tctatgactg
    10681 tagaagaaat ctatcaaaat cgcgataagt tcagtcaaag cgtacaagaa gtcgcttctg
    10741 tagaccttgc aaaaatgggg ttgatcatcg tttctttcac tatcaaagag gtcagagaca
    10801 aaaacggtta tcttgactct ttagggaaac caagaatcgc ccaagtcaaa agagatgcag
    10861 atatagcaga agcggaagct ctaaaagaaa cgcgtatcaa aaaagctcaa gcagaaaaag
    10921 aatcacaagc agctgaatta cagcgacaaa cagagatcgc agaatcatta aaagaaaaag
    10981 aactgaaatt agcaacctac aaacaagaac aagatgttgc taaagcaaaa gcagaccaag
    11041 cctacaattt agaaagtgcc cgagcacaac aacaagtaat cgaacaggaa atgcagatta
    11101 aaatcgtaga acgtcaaaaa caaatcgaac tggaagaaaa agaaatcaca cgtcgtgaaa
    11161 agcaatatga ttcggaagtg aagaaaaaag cagatgccga tcggtacgct aaagaacaag
    11221 aagcacaagc tcaaaaagtc aaagaagtga ctgaagccga agcagaacga ttccgtgtgg
    11281 aagcactggc agaagcggaa gccaataaga cacgtttagc tggacaagca gaagccgaag
    11341 cagcactagc aaaaggaaaa gcagaagccg aagcaaaaca aaagatcgcc aatgccttca
    11401 aggaatatgg cgaagcagct gtcttgagta tggttatcga tatgctaccg caattgatgc
    11461 gcgaagcagc acagccgcta ggaaatatcg aaaaaatctc tgttgtcgat acaggaagca
    11521 gttcaggaga aaccagtgga gcgaaccgag tgacaaatta tgccacaaat ttgctttctt
    11581 ctactcaaga aacactaaaa gcgacaacag gacttgactt gaaggaaatc gtagagaatt
    11641 tgtcgacacg tgggactact agccaagtaa atatgtatga agcgccaaaa gaggcagaac
    11701 aaaactcgat gaaggaagaa taaagtagta cacttcaccg ttcaatcaaa aatggaggaa
    11761 gactttttaa agttcttctc tccatttttt tattttattg caaaagaaaa ttagtcctcg
    11821 ctatttttct tttgtttttg gatcttagct tctatctctt gtaaatttgt tcttagcaat
    11881 cgttctttct tttggagttc gaccaaatct tctgatagtt cttccttctt ttctgtttca
    11941 ggaggacgaa gaaagaaatt gataccgatt gcaatgaatg tccctataaa agtatctagt
    12001 acacgttgga tagcatacaa agcggattcg ccttgaggga tgctcaacgc aatcagcaac
    12061 aacgtagcta ttgcggaaat gataccggaa ttattgttaa taccatctga cacgacgatc
    12121 acaataataa ctagaatagg aagcaagatc aattctacca aaaaatcatt gtgaaaatag
    12181 cttttgacaa aaaaataaat gatagctaat gtaccaccta atgtattgcc gatgatccta
    12241 gactttccaa aagaaacggt tgtagtcaag tcctgacgca aagaaaaaac agcagataat
    12301 gcagcaatca tcgggattcc tcgttgagtc aaatgaaaaa acaagataca taaaaaaaca
    12361 gctaaggcac ttttgatcgt acgcatacct agtcgaaaac ggccaaattg catagagatt
    12421 ctcctttttg gtctttttac tatcatacct aaacttttgt ctgatagcta gtttttcatt
    12481 attcaagtat aattaaaata aaaaaggggg aagtaaatgc gtataggaat gcgaacagtc
    12541 aaaacagtga tcagtgtctt cttttcgatg gtcacagcgg gtagcttatc gctgttgtat
    12601 tggccagctg cagggatcat tgcattatta agtgtaggaa ataccaagcg ctctacattg
    12661 cgtacgggca tgaatcgaat cgcagccttt atattagcta cggcaacagc actgttcagt
    12721 ttttttgctg ttgggtatac tatttttggc ttcagtcttt ttttattcct gttcatcccg
    12781 cttgcctctc gcttcaaatt aaccgaaggg atagtagtaa attcggtttt ggtcactcat
    12841 tatttagtgg aggaaaacat gtcttggcag ctgatcggca atgaagcggg attaatggcg
    12901 attggattag tatttgcttt gcttgcaaat gtttacatgc cagatcgtac gaagcagctg
    12961 aaggaaaacc aaatacagat cgaaaaagaa tttcgaaata ttttgagaca aatggcagaa
    13021 tttcttttat cagaaaataa agaagatgtg cagatcaaat gcgaacattt actgacattc
    13081 attcgcgaaa gccaagaaga tgccagagaa catcaagaaa actactggct cagacagccg
    13141 ctatactacg aaacttactt ctctatgaga agagcacagg ccaatgtgat taaagacatg
    13201 ctggaaaatc tagagagaat ccagcaacca gcttattatg gaaaacatat ctatggttta
    13261 ttgatttata ctgcagaaac attttcagag tcaaatgatg gcagacaaat tttaacacgt
    13321 attgaagaag tttatgtatt gtatcggcaa atgcctctgc cgactagtcg ccctgaattc
    13381 gaggatcgag cagaattgtt ccagtttttg cagtctttta aaagttttat tgaaatcaag
    13441 gcagaattct cccaacaaaa aatccaaaaa tagaggttgt gaattcagct aatattcagg
    13501 tcaaaaagct gatttcacaa cctcttcttt aaacggtgtt ctcctgtcaa ctcctcggtc
    13561 acaagtcaca atattcaaca aacatgagaa gctgtttttt gaaattgctc cttgtgactc
    13621 ggagctgaac ggtaggctca caacctcttt cttaaacggt gttcaaaagc tttcctctgc
    13681 ggtaataagt ccagccaaac gaaaaatagg aggagctatt tttgtttggc tgttcttatt
    13741 acttgatgca gaacagcttt ttcacaacct tttaaaatta catatttttc catagaactt
    13801 gcatttgatc caagatttcg atgacatctt ttgtcaagaa gagagagcga ttgggcaagt
    13861 caccttcaat catgtgtacc atattcttta tttcgtaatt cattgcttca gatgtattcc
    13921 cgctttcgat gaattccttt gtaccatctg taaataagat ctccgcacga tcagcacgcg
    13981 ggtaatcagc aatcgtcaca taaccgttct caaaggcaac gataccgact tttggcattt
    14041 ttgcctgaaa agtcaagctg acagcagcaa gttcattttc tttgttacgc aagatagtga
    14101 cggattgttc gtccacgcct gtttcaaaag gaaccattgt agaggcaact acttcaggtt
    14161 gagagctcaa aaagaaacga gcgaaagata cagcatacgt accgatatcc aacagagctc
    14221 ctccagctaa ttctggattg aagaaccggt ttttagggtc tggttctttg taactgccga
    14281 aaggtgcttg gatcattttt aaagccccca gttttcctgt atccattatt gagcgtaact
    14341 gctgataaag tggcatgttg aagattgtca tcgcttctgc taagatcaaa ttcttttctt
    14401 ctgctaagcg catagcgtca gccagttctt ttttattcat cgtgatggct ttttcacata
    14461 gaacgtgttt gcctgcttcc aatgctgcca agatatggtc gatatgctgt ctatttggta
    14521 cagcaatgta cacgatgtcg atttcagcat cgtctaacat ttcttgatag cttccgtaag
    14581 cttttggaat attgtaacgg tgagcgaagt tttccgcttt ttctgaagtt ctggaagcaa
    14641 ctgctgttag ttcgctcgtc tcttgattga agctttcggc aaactcatgt gcgatggtac
    14701 caagaccaac gattccccat tgatattttt tcatctgaaa acgctccttc atagttggat
    14761 tgagtttctt gtctgtctat tattataaca aaaatcacga agaataaaga ggagaggact
    14821 tgaaacatta aaaaaaatga aagcagcgat ttaagaggtg ctttttgatc aaaacttaag
    14881 tagaatagtt aaaaggaggg gataaacgtg catcaagtat atattgatct tattatgagt
    14941 gtattattag aacagtttga aactgaaaaa aattttttag atgaatactt gctgatcagt
    15001 ccagaacaat ggcaaaaatg gaaagatggc gagaaaaatc tgccatctga agcgatgcag
    15061 aaagtaaaaa atcttttcac tgattatgaa tggatgctaa cgcaaaagat tttgcgtcaa
    15121 acgattattt ttcccgaaaa acgaaacgtt gccgttgcag agtacaagca aatcaaaaca
    15181 cgcatcgcgc aaaaatggct tagcagcaaa gcagcaaaag tcgagctgat ccctttgaaa
    15241 aataatcagc aggctggtag ataccttgac ttgaaagtaa gtattcaata tgatgaatgg
    15301 ggctatgatg atattttgaa ttttcgtctg ccagcagatg ttcaaaaaca gatcgaagga
    15361 gaaaaaatcg aacttctcga ttgggtgaat gaaaatctgg aagaaactta cgttcatgag
    15421 gcgaatgggg aacgtgaaga acaacgaaag gatttttcaa gtgaataaat ttttaggatt
    15481 tggagcagtt gtgattatga tcgcagcaat gggctatgtt atttttagcg atgatgattc
    15541 gcatgttcaa gccggcaatc aagcagtcga acgaacaagc accaaaacaa ctgctagtaa
    15601 agaaaagaac agtcaaacaa ccagcagcaa ggtaaaggac aaagaaacaa aagatcgtac
    15661 gtccaaacaa ttgccagatg ttaaaacaga cgattggaag ctagttttgg ttggtccaga
    15721 tcacaaatta gaaaaagaaa ttgatgagtc tactcaattg gcatcattaa gtaacggata
    15781 cttgatcgac aaacgcattg ctccagaata tgaagcattt gccaaggctg ctgaagcagc
    15841 tggatttcca ttagtgatgg tctcagctta ccggtctgtt atcatcccaa caacaagttt
    15901 tcgaacaaaa tgtccaagat gtgatgagtc gtcaaggtgt atcagaagag gaggcgacaa
    15961 agattactaa agaaacgatt acggagccag gatacagcga acaccacaca ggtctggcag
    16021 tcgatgtcgt cgatcaaaac tggtacaaca gttatccttc tactgtattg gatgcaagtt
    16081 acggagatca gcctggagct aaatggatcg cagataatgc tgcgcagtat ggatttatcg
    16141 ttcgttatcc aaaaggacac gaagatataa ctaagatcac atatgaacct tggcacttac
    16201 gttatgtcgg taaagaaaac gctgagtata tcaccaaaca ccaattgacg ctagaagaat
    16261 atctgaatga attaaatgaa aagtaggaat tgatcatggc gaaaaaaaag tggaaaaagc
    16321 aaaagcgcaa gaatatccgt tggccagcag tggttgccgg tgtagcagtg atactcttag
    16381 cctttgtttt ttccttgaag aatctgtctt ctccttttac tgataatcag caagaaaatg
    16441 aagcgcaatc ccatcagcaa tttattgaac ggcttgttcc gcatgcccaa gaactacaag
    16501 atggttatgg agtgcttcct agtatcattt taggacaagc tatattagaa tcaaattggg
    16561 gaaaaagtac cttggctagc aaatacaata atcttttcgg gatcaaggct tatggggatc
    16621 agaagaaagt aagcctggaa acaaaggaat ttgttaacga agaatggata actatccaag
    16681 gagacttcaa agtgtatgat tcatgggaac agtccatgga tgaccataca caactgttcg
    16741 tccaaggagt agattggaat cctgcgctgt atgaaaaagt gattacggcc accaactatc
    16801 aggaagccgc acaagcattg caagatgcag gttatgcgac agaccccggt tatgcgcaaa
    16861 aaatcatcca ggtcatcgag acgtatcaat tgaatcagta tgaccggaga tagcaaagga
    16921 gaagtggctg atggcagaac aatttacgcc agaaatcatg acggaattga gaaaagatat
    16981 cgtaagagta ccggaaatca ttttagaagc aagcgggatc aagatatttg gaaaattgat
    17041 ccgttcgatc atctttacta cggatattgc gatcatacgt aatacaaatg cgaatgcagt
    17101 aattgctgtg tatcctttta cacctcatcc agcgattact aaaagcatta ttgaggcagc
    17161 tgacattcca gttttctccg gtgtaggtgg tggtttgaca caaggtgctc gttcttccta
    17221 catgagcatg ttcgcagaag cgcaaggttc gttaggggtc gttttgaacg gtcctacgcc
    17281 tttagagaca gtaaaagaag tttgtcgagt agttgatatt ccagttatca gtacggtaac
    17341 gtcgaaatat acagaaatag atgaaaagtt agcagctggt atttcgatta tcaatatcag
    17401 tgcaggtaaa gaaactgcag aagcagtacg ccattttcgt aaaaaatatc ctgaacttcc
    17461 tatcattgcg acaggtggtc caacggaaga aagtattgct gagaccattc aagcaggagc
    17521 aaatgccatc acatatacgc caccaagtag cggagagctt tttagtcgta aaatggaaaa
    17581 atatcgggaa actgagaaaa agaatcatga ataacaggtg agaatcaaaa gacgaggaag
    17641 agcagatagt ggctcttcct cgtcttttgt actccagtac tatttgttca gaattcaaaa
    17701 aaaagagaaa tttttttgtt tttttacctg taatgtacat aagaagagtt aaaacctttt
    17761 gttattatta gcattctaac aaaaaaatgt cttgatttat tgaagcgctt tctatagaat
    17821 gaaaaacgaa taaaaactaa aaaaggcacc cgacaattta gtagcagcta aattgtcggg
    17881 gcttgaaaag tcagcgggaa ctgaccaatc aagttgccat agtcaatgct tgtgcatcga
    17941 cgtgtatatt gtacctaatt tcatacagaa ttactagtta tttttctgtt ttttagggaa
    18001 gagcgcacac gaagaagcat gcttatttag ctatgtcaag gatattgggc agttgtctga
    18061 tatccttttt tctttgcgta attatcctcc catcattgaa gcaagaagtg tttttagcga
    18121 tgaaagaaga atcagcacta tctaattttt cttgaaagta ttgagttgtt catcctaggc
    18181 agaacgataa ggcaagtctt ggtttttatt ccccttgaaa aagatcgttt aataaatatg
    18241 ttctgtataa aaatacagta tattgaaaaa taacaatgga agtagaggtg cagagaatga
    18301 agaaaagaat gagaatcatc ttagctttaa gtgttatgtt attcttcttg acggcttgtg
    18361 ggcaagaaga tgacaaaaaa atcacgctga acatggcttg gtggggttcg caagtccgac
    18421 acgatgcaac agtaaaagta atcgagcttt atgaagaaaa aaatccccat gtgacgatcg
    18481 attatgaatt ttatgattat gaaggatatt acacaaaatt aggtacacta gcagcatcag
    18541 atgatatctg ggacattttc caactgaaca atcaatttcc tcaatacatc aatcagattg
    18601 aaccattaga tgaatatgtg gagcaaggga taattgatat ttctaatgca gatgaatcct
    18661 atttagcaac gacgacttac gacgggcaat tagttggact gtctaacggg gtgaattcgt
    18721 ttgcgattgc ttacaataaa aatatatttg aacaattaaa tatccctgaa ccagcttcaa
    18781 attggacatg ggatgaattt gaacaaatca gctatcaaat cactgaagaa ttaggaatct
    18841 atgctatttc aagattcgaa gattttatag ctggctgtat tattcaaata ccacaagttg
    18901 agaaaggatt gaatttttat aatcgggaag atctatctaa cagcttggga tttgaaaatc
    18961 cagactatct aacagatttt ttccagctgc gtaaaaatct ggtagacaaa ggggcccatc
    19021 cagaaccagg tggtggagct gcaagtacga cggatctgtc ttttcaggcg gttagggata
    19081 gagaagcggc aatggtgttt ctatcaagta accagttgac agaaatattg tcaggggctc
    19141 ctgaagatat ggagattcag ttgattaatc cacctcgcag aaaagcagat ggagaatctg
    19201 gtatcccttt acgttcttcg caaatgctga gtatggcaaa aagctcagcc cataaagaag
    19261 aagctgctaa gtttattgat ttctttcaaa acagtgaaga agccaatgaa attttaaaag
    19321 gagaacgtgg tgtaccaatc atgtcgaata ttcgagaaca gctcgctcgt gcagatgatg
    19381 caactttgca agcgacattt gattatttag aaatgatttc cgatatggac aacggcgaag
    19441 tgaatgtctt tgatagtgct ttgaatccgg aaattcaaga ccaatacaat cttttagttg
    19501 aaaaagtcat ttatgaagaa gtaacagcag aagaagcagc agagagtctt tatgagtttg
    19561 cgtcaaacat tctaggatag cagcaggagg aaggcatgaa aaaagaaaaa ctaaaacggt
    19621 tcttggatca agacaatgtg gtcggttacg tatttgcttc tccattcatc ctaggatttc
    19681 tattgataac agtgataccg atgatcttgt cactttatta ttcgttcacc gattatcgtt
    19741 tgggaaatcc agtctcatgg atcggcttgg ataattatat aaagatgctg agtgatgccc
    19801 gttttttgaa ctccatccga gtcacttttc tctatgtatt tgcatctgta cctgtgaaac
    19861 tgatctttgg tctattgatt gcttatcttt taacgaggaa tatcaaagga acgactttct
    19921 ttcgttcttt gtattatgtg cctagtttga tcggtggcag tatttctatc gctctagttt
    19981 ggcgggagtt gttttctaaa aacgggttag tgaatatttt cctggaacga ttaggcgcac
    20041 ctgcgctttc ctggtttagc gatcaaaaac tggtcatgat ccctcttgtt ttgatgtctg
    20101 cttggcaatt cggttcggca atgatcatct ttgctgcagg tatcaaagag atcccaggct
    20161 cgtattatga agcggcagag atcgatggag cgaacaaatg gcagacgttt tacaagatca
    20221 cgttacctct tctttcgccg atcattttgt ataacttggt tatgcagaca atcgcggcgt
    20281 ttatgacttt tacgcaggca tttatcatta cccaaggtgg tccgaatgat gccacgaact
    20341 tttatgctct ttatgtgtat aaccaagctt tttcttttaa ttctatgggg tatgctagtg
    20401 ctatttcgtg gatcatgctg ttgattatgg gagctgtcac ctttctgatt ttcaaatcat
    20461 caaaaaaatg ggtcgttgtg tgaatgagag gaggaagaaa tgaataatac agtaacgaaa
    20521 tggtttcaat acggttttat tttgctatta ggtctagtca tgatttatcc ggtcttatgg
    20581 atgatcgcag gttcttttaa aacggatcaa gagattatga gtggttcgct gaatttgatt
    20641 ccagagactt ttcaatggga aaattacgca aaaggatggg cggggtttgc tggaatttca
    20701 ttttttactt tctttaggaa ttcgtttgtc atctctgcag tttcgacgat agggacagtt
    20761 ctgagctcga cttgtattgc ttacgcatta tcgagaatcg atttcaaagg aaaaagattc
    20821 tggttcgtcg tcatgttagt aacgatgatg attcctgcac aagtgatttt gattccgcag
    20881 tttattattt acaatcggct gaatttggtt gggacgtatg tgccgctgat tttaccacat
    20941 ttttttggac aagcattttt catttaccag atcatgcaat tcatggtcag catccccaaa
    21001 gagttagatg aatcagcgat tattgacgga tgcagcaagt atagtgtctt taccaaaatt
    21061 atttttcctt tgctgaaacc ttcgatcatc acgacgatca tcatccagtt ttattggaaa
    21121 tgggacgact tcatgggacc attgatttat ttaaacaagc cgagatctta cacggtatcg
    21181 attgccatca aattatttgc agacgctgga tctagtacga cttacgcgag tatgtttgcg
    21241 atgtctacgt tgtcgctgct accggttttt ctgattttct tattcttcaa taaatatctt
    21301 gtccagggga tcagtacaag cggtctgaag ggctgaatat gaaaaagcca atagaagaga
    21361 tgtttcttta caagctcaac tactatgtct gtcggatttt tttaaccagt cttatcagta
    21421 atgctctttt cgctttagtt ctaggatgtt tgttcacttt aggattagtg ctgtctgctc
    21481 gtgttgtcta tctgatttat tttttgatga tgtatccttc ttattatcta tctgtccatt
    21541 acttggcaga aaaaaaggta tgttggagga aaggaaaaat caaacggatg tgtttcatca
    21601 ttacgttgtt tcagctgttt gtcattattt ttgcagttga ttggtattta ttagtgggaa
    21661 agattggagc ttggtatctg attccttttc tattgtgtct gttgcttttt gtatcccata
    21721 gtttttttag tacgctaatt gatatctgca aagaagataa agcagatagt cttgtttatt
    21781 tgatcaaaag gaatatctgg ctgttgtcaa aaaacgtcaa ggggtttctg tggtatttat
    21841 gccttttgta tggactgttc tggtgtatac agcatgattt tttactcact gcattactgt
    21901 atccagggct gccatttgtc acagtatttt gcttaaaaga aaaggagaat gactttaaat
    21961 gaagaaaaaa tatgtacaag tcggacttgg tgggagagcg cgtttttttt atgaggcaat
    22021 tgcctctacg tatcatcaaa cttgtgaact agtcggtttt tgtgatgtat ctcaaacaag
    22081 gatggattat gcaaatcgga tacttgtgaa caagtatgga cagaaaaaag taaagacatg
    22141 tatgccatca caatttgagc aactactgga tgacgtacgg ccggctgaag tcattattac
    22201 gtcagtagat cgaacgcatc atgattatat cattcgtgca atgaaaaaag gatatgatgt
    22261 tatttgcgaa aaacctctga ctatcgatga ggaaaaagct caagaaataa taacggtcca
    22321 aaaacagacg ggaagaagcc tgcgagtcac ttttaattat cgttatgcac cgtatcacac
    22381 gaagataaag gaactgatag aaagtgatgt gattggtgat gtgttttcta tccacttcga
    22441 atggctgtta aatacgcaac acggggcaga ttatttcaga agatggcatc gaaataagca
    22501 caatagcggc gggcttcttg ttcataaatc tacccatcat tttgatctag tgaatttttg
    22561 gttgaacagc cgtccccaaa aggtatttgc attcggtgga ttaaactttt atgggatgca
    22621 taatgcggag aaacgcggag tagaaatatt ttacagtcga tgctatggaa gcgaactagc
    22681 taaagctgat ccatttgcta tagacctgtc agccaatgag gaattgcgag aaatgtattt
    22741 agaagcagaa aaagacagtg gatatattcg tgaccgcagt gtattttccg atgatatctc
    22801 tattgaagat acactaggcg taacggtcaa atatgaaact ggtgcgattt tgacgtattc
    22861 gctaaacgcg tatatgccgt gggaaggatt caatgtttct ttcaatggca gcaaaggacg
    22921 gatcgaaatg aaagtgatcg aaaagtctta catcaatgca ggtggagata aaaaggatga
    22981 aggagcagcc gttcttcagt ccatccatgt ctatccgatg ttcggtgagc catatgaggt
    23041 aatcgtcgaa caagaagcgg gcggacatgg cggaggagat ccgcgtttat tagatgatct
    23101 atttggtgaa gaaaaagaag aagatccgtg gaatcgggca gcaactcacg tagatggtat
    23161 tttgtcgatt ttgacaggaa tcgcagcaaa tcattctatt gcttcaggaa aagcagtagt
    23221 gattgacgag cttgtcagct taaaagaata agaatagatt tacggaaatt acttcccgta
    23281 gtttataaaa tcttgcctta ttgccgatag gctgtctttt gaacactatt tattcggaaa
    23341 aaggggctat gacatgcttt ttgtcatgcc ttctttttat gtgggctgct tttttagata
    23401 ggagaaaata aaaataacta tttaaacccg aacaaaaagg aataatgcat taaaaatcat
    23461 tagattttac aaaaaaataa gtaaaccacg ttgtcagttt cagaaagtag tgttagactg
    23521 ttttcaatat ttgaaacaac attttttatt caaatatggc aaactgcctt cacgtgcaaa
    23581 acagttgcaa gaaaatgaat ttacactatt attagtttta catacctact tatatatcgt
    23641 cataatatgg atgacagttt ctagccagta ccgtaaatgc tggactataa gtaaaagatt
    23701 ggctatttca cccttctttt cttatgataa agtggagggt gtttttcttt aacagaaaca
    23761 tagtaggttt ttgcacaaaa gcggttattt cggtattaat aaatgataaa ggagcaaata
    23821 aagtgaaaga attagttgaa cgtattcaaa aagacggacg tatattaggc gagggcgtat
    23881 taaaagtcga cagcttcgtc actcaccaag tagaccctgt attgatggaa caaatgggaa
    23941 aacggtttgc agaagtattt aaagaacaag gaatcacgaa agttgtgacc attgaagctt
    24001 ctgggatcgc accagcttta tttgctgcaa gagaattaga tgttccaatg atcttcgcta
    24061 gaaaagcaaa aagtttgacg atggatgaag aattattgac agcttctgtt tattctttta
    24121 cgaaacaagt gacaagtacg atttctattt ctcgtaaatt tttatcttca gaagacaaag
    24181 tattgattat cgacgatttc ttggcaaacg gacaagcggc aaaaggattg atcgaattat
    24241 gccagcaagc tggagcgcaa gtagaaggaa tcgggatcgt catcgaaaag tctttccaag
    24301 atggacgtca gttacttgaa gaaatgggat tgagagtcgt ttctcttgcg cgtattgctt
    24361 ccttgacgaa tggacaagtt gagtttcttg aggaggacgc ataatcgtgg aaaaagaaac
    24421 acaaaatgga aaagctgcgg ttttagggct gcagcatctg ctagcgatgt atgcaggagc
    24481 agtagcggtg cctcttttga tcggtacagg actaggcttt aatcaagaac agatgactta
    24541 tttgatttca attgatatct ttatgtgtgg attagcgaca ttattgcaat tgacagttaa
    24601 ccgatttttc ggaattggct tgcctgtcgt tttaggatgt gcgatccaag cagtagctcc
    24661 actgatcttg atcggaacca atgaaggagt aggagctatc tacggatcta ttatcgcttc
    24721 tgggatcttt gttgtgttag tttctggtgt cttttctaaa attaaaaaac tattccctcc
    24781 tatagtaaca ggaacggtta tcaccgttat tggtttgact ttgattcctg tagcaatcga
    24841 gaaaatgggt ggaggtaatg caacagcagc tgattttggt gacaaaacaa atctgctact
    24901 tgcatttgtg acgattctcc taattgtagg cgtgcaaatg ctggcaaaag ggttcattcg
    24961 ttcaatcgcg gtattgattg gtttagtcgg aggaagcatc ttagctgctt tcatgggaat
    25021 gattgatctg agcgcgattg gagatgcgcc gttgttccat gttccacaac ccttttattt
    25081 tggtaaacca actttcgacg tatggtcgat tttactaatg atcatcattt ccatggtcag
    25141 catggtagaa tcaacaggtg tatattttgc acttggtgac attactggga aaaatgttgg
    25201 agaagaagaa ttgaaaaagg gatatcgtgc agaaggactt gccgttatct taggcggtat
    25261 tttcaataca ttcccttata ctggcttttc tcaaaatgtc ggattggttc aactttcggg
    25321 aatcaaaaca agaaaaccca tctatttctc tgcattcttc ttgattgttt taggattatt
    25381 ccctaaaatc ggtgcgttag cacaaatcat ccctgagccg gtacttggtg gaggaatgtt
    25441 agtcatgttc gggatggtgg cagtccaagg aatccgtatg ctgttggaag ttgatttcag
    25501 caatgataaa aatctattga ttgttgctgt ttctgttgga tttgggctgg gtttcaatgt
    25561 tacgccgaca ttattcagtc aaatgccgca aacattgcaa atgttcactg gaaatggcat
    25621 cgtaatgagt agcattacgg ctatcatatt gaatttgatc tttaatggat tgaaaaaaga
    25681 tgaagaaata ttggaagaag ccttcaagaa gtaatagagc aaaagaaagg ttgtgaaatc
    25741 agcgttttga cctgattgtt agagaaacaa acggaaaaat agttcctcgt attttgccgt
    25801 ttgtttgagc taataccgag ggtcagctga ttgaacaccg ttgaaggaag aggtcgtgag
    25861 cctgccgttc atctccgagt cacaaggaac aatttcaaaa aaaagcttct catgtttgtt
    25921 gaatattgtt ccttgtgacc gaggagttgg caggagaaca ccgtttatcc ataaaaagga
    25981 atgtgaccta acttttgtca cattcctttt ttttatcttt aataaacacg aacaataaaa
    26041 tgtttttctt tgttaacatt cgaaatttct ttgacatcaa aaaaagtgat tgttaaaata
    26101 tgaatgtaga aaaactggga ggaaaaacaa tgacagcaaa aatctctgtg attatgggga
    26161 gtacttcaga ttgggaaaca atgaaagaag catgtcaaat attggatgaa ttcggtgtgt
    26221 cttatgaaaa aaaggtcgtt tctgctcatc gaaccccaga tttgatgttt gaatatgcac
    26281 aaaacgctcg aaaaaaagga atcaaaatca ttattgcagg agcaggggga gccgcgcatc
    26341 tgccgggtat ggtcgcatca aaaacaactt tgccggttat cggtgtaccg gtccaatcac
    26401 gaacattaaa tggtttggat tcgcttcttt cgattgtcca aatgcctgga ggagtacctg
    26461 tagctactac agcaatcgga aaagcgggag caattaatgc tggactactt gcgatacaga
    26521 tgctttccat gtatgattta gaattggagg ctaaattggc tgaaagacgt tcaatgttgg
    26581 ctaaaactgt tatagaaagt agtgatcaac ttggttaaac ctttgttgcc tggttcaacg
    26641 atcgggatcg tcggtggcgg tcaattaggt cgtatgatgg cgattagtgc aaaagaaatg
    26701 ggctttaaag taggtgtgct ggatcctgtg agcgattgtc cagccgcaca agttgcagat
    26761 tggcatattg aaagtgtcta tgatgacacc tttgcgctga aagagcttgc cagaagaaca
    26821 gatgtcatta cctatgaatt cgaaaacgtc agtgtagagg cgttaaatgc gattttaccc
    26881 atgtcattta ttcctcaagg gacagacttg cttgcgatta cgcaagaccg cctgttagaa
    26941 aaatcttttt tggaaacgaa taacatcgtg attgctcctt atgcaacgat cgtcagtccg
    27001 acggatatcc aagatgcgat tgacgggatc ggctatcctt gtgttttgaa aaccactcga
    27061 ggcggctatg atggcaaggg gcagtatgtt ttgaaaagtc gggcggatct agctcctgcc
    27121 atggaccttt taagagaagg aacctgtgtt ttagaagcat ggatcccgtt tgaaaaagag
    27181 atttcgatca tggtggcagg aaatggtcaa ggagatttca cgacttttcc ggtcgtggaa
    27241 aacatccacc acaacaatat tcttcatgaa acgattgctc cagccgcgat tgatcaggat
    27301 gtgatcgagg aggctgagcg tattgcccgt gtgattgcag aagcagtgag cctttcaggt
    27361 gttttagggg tagaaatgtt tttaacaaaa acaggcgggc tatatgtgaa tgaattagct
    27421 cctcgtcctc acaattctgg acattattct attgaagctt gttctatgag tcagttcgat
    27481 gcccatattc gaggtatttg tggttggcca ttaggagaaa aagtacgttt gataagcaat
    27541 gcagtaatgg tcaatgtttt gggagaacaa ctgctggaaa gctatcgctt gattgagaat
    27601 aagccagact ggcaattcca tttctatgga aaaaaacaag caaaaaaagg tcgcaagatg
    27661 ggacatatca ctatcttgac agaagacatc cagcaaacgc tggaagaaat caaacaaacg
    27721 aatatttggg attagaggag aaaaaacatg ttagaacgtt atacacgccc tgaaatgggg
    27781 aatatctgga cggaagagaa caaataccgt gcgtggctcg aagtcgagat tttagcagat
    27841 gaagcttggg cagaacttgg tgaaatcccg caagaagatg tgaaaaaaat tcgtgagcat
    27901 gcatcatttg atatcgatcg gatcttggaa atcgaaaaat taacacgcca tgatgtcgtt
    27961 gcgtttacac gagcggtttc tgaaacattg ggagaagaaa gaaaatgggt ccattatggt
    28021 ctaacaagta cggatgtcgt agatactgct tatggctatc tcatcaagca agcaaatgat
    28081 attttacggg aagacttgaa acgttttacg gaaatcattg gcgaaaaagc aaaagaacac
    28141 aaatatacag tgatgatggg tcggacacac ggtgtccatg ctgaaccaac tacttttgga
    28201 ttgaaacttg ccacatggta ttcagaaatg aaacggaaca tcgaacgatt tgaacatgcg
    28261 gcaaaaggag tagaagcagg aaaaatctca ggagcagtgg gaacgttcgc aaatatcccg
    28321 ccatttattg aagagtatgt atgtaaacaa ttggggatcc gtccacaaga aatttctact
    28381 caagttctcc ctcgggatct acatgctgaa tatttctcag cgatggcgct tattgctaca
    28441 agtattgaac gttttgcaac agagatacgc ggattgcaaa aatccgaaac acgtgaagta
    28501 gaagaatttt ttgcgaaagg tcaaaaaggt tcttctgcta tgccgcacaa aaggaatccg
    28561 atcggttcag aaaatatggc tggtctagcc agagtggtgc gcggacacat ggtcactgcc
    28621 tatgaaaatg tcacattatg gcatgaaaga gatatttctc attcttcagc agaacgaatc
    28681 atcttgcctg ataccacgat tttattgaat tacatgttaa atcgttttgg caatatcgtt
    28741 aaaaatctaa cggtctttcc tgaaaacatg aagcggaata tgtctgcaac atttggtttg
    28801 atttatagcc agcgcgttat gctgaaattg atcgataagg gaatgacaag agaagaagct
    28861 tatgatcttg ttcagccaaa aactgcgtac gcttgggaca accaagtgca gttccgcccg
    28921 ctgttagaag aagatccaac catcacatcg gtattatcta aagaagaaat agacgatgcc
    28981 tttgattatc atcatcattt gaaaaacgtt gacgtgatat tcaaacgagt aggtttggaa
    29041 taaagaagag tgagttgcaa aattcaatta aaatagaata gcaaaaataa ccaaaaaaga
    29101 agcagatccg ttagcggttc tgcttctttt agtttattat attttcttat caataatagg
    29161 aacctgattt taaaagcggc tacctcttta ccgtgcctct ttacatacaa aaaatagagg
    29221 aattaagcgt aaatggcttg gctattataa ctaaaatgct ggttatggag atgttgcagc
    29281 aagttttcga gaaaagcgta ttccatctct tgtccattga ataagatctg gatacgataa
    29341 tgatttttga aacgcttctg ttcatgaatc tgactgattg aaagttcgcc tttgtgttct
    29401 gtctgataaa gaaaagtcct tttacggcat tcagcacagc gcttggttgc tcggcggatt
    29461 tttcggaatg tataaaaact agtatcttta ttgcataaat aaacgtcttt gatcgacagc
    29521 aaatcattat atagacagga acgttcttca gctgttaatt ctgataaaaa aagctgacct
    29581 tgttttattg catcttccat cggcaagtgc ctcctcgaac ccattatcca aatttttagt
    29641 taattctaat ataccatcat taagtataaa tgtaaacgct tgttgatggt ataagatttt
    29701 cgtttctgat aaaacacgaa cattaaaaga atctaatgcc ttaaatgtac atatttttgt
    29761 tgatttataa aaagatgatt gctaaaattt aaagtgaaaa aacgaacaaa aaacgatgta
    29821 tttgctttta atgtaaataa aaaggagaga cgtacatgga gaagaaagaa cttttgtatg
    29881 aaggaaaagc caaaaaaata tatcttactt ctgatgagaa cttgatttgg atagaatatt
    29941 tagatcaagc aactgcgttg aatggcgcga ggaaagacca gataagtgga aaaggagaat
    30001 tgaataatca aatcacaagt gcaattttcc gttatctgac tcaaagagga ataaaaaatc
    30061 attttattga acaattgtct aaaaccgaac aattagcaaa aaaagtacaa attatccctc
    30121 tggaagtggt tatccgaaat attgcagcag gaagtttctc aaaacgtcta ggtgttcccg
    30181 aaggtaaaga attcatcaaa ccaatcattg aattctacta taaggatgac cagttggatg
    30241 atccatttat caatgaagag catatctatt tcttacaact tgcaaagccg gaagaaatca
    30301 gtgagctgaa agcccaagcg ttggctatta atgaaatttt gaccgaatta tttgctaagg
    30361 tcggtttggt tttagtagat ttcaagctag aatttggccg gtcgaatgga caaattttgc
    30421 ttgcggatga agtaacaccg gatacttgcc gattatggga tgaacgtacg aatgaacata
    30481 tggacaaaga cgtttaccgc agagatctag gtgatttgat cccagtctat caagaagtat
    30541 ataaccgtct atcggaacta actgaagaaa aggagcgagt atgatgtatt tcgtaaaagt
    30601 atatgtaacc tataaagatt cagtgttaga tcctcaaggg gaggcagtaa aaaaagcagt
    30661 ccatcgtatg ggatatgaaa caatcgaaga tatccgaata ggaaaatact ttgagataca
    30721 acttgctgat gcagaagaac caatcgatac agtgattgaa acgatttgtg acaagctgct
    30781 tgcaaatgta aatatggaaa cataccgata tgaaatcaaa aaaatggagg aagtataaca
    30841 tgaaatttgc ggtcatcgtt tttccaggtt caaattgtga cgcagatatg ttatgggcag
    30901 ttcgcgagat catgggcgct gatgccgaat ttgtacggca tgatgagtcc tctttagaag
    30961 gatttgatgg ggttttactg ccgggaggat tctcctatgg tgactatttg agatgcggtg
    31021 cgattgctcg gttttctaca atcatggaag aagtgattcg ttttgctgaa gaagggaaac
    31081 cagtattcgg tacatgcaat ggattccaga tattgacaga agcaggactt ttgccaggtg
    31141 tactgagacg aaatgagtct ttgcatttta tttgcaaaac tgtaccattg aaagtcaacc
    31201 ccaagacggc ctttacttca gagtatgaag ccgacgaaat aatctatctt ccagttgctc
    31261 atggggaagg aaattatttc tgtgacgaag aaacacttca agaactaaaa aaaaataatc
    31321 agattgcctt tacttataat gacaacatca acggcagcgt ggaaaacatt gcagggatta
    31381 cgaacaaagc agggaatgtg ttaggaatga tgcctcatcc agaacgtgca gtagaagact
    31441 tgctaggatc agaagatggg aaacggttct ttgcttccat tttaaaaaac ttcggaaagg
    31501 tgaccgtata gatgaaaaca ttgaaagaac cgacagctga agagatcaag aaaaaaagaa
    31561 tctatgccga atggggattg actgatgaag aatatcgaat gatcgaagaa gatatcttag
    31621 gaagaatgcc taattatacg gaaactggac tgttttctgt tatgtggagc gaacattgtt
    31681 cctataaaaa ttctaaaccg gttctacgca aatttcctac tagtggacct catgtgttgc
    31741 aaggccctgg agaaggcgca ggcatcgtcg acatcggtga tggactagca gttgtcttca
    31801 aagcagaaag tcacaatcat ccttcagccg tggagcctta tgaaggagct gcgacaggag
    31861 ttggcggaat catcagagat attttcagca tgggggctcg tccaattgcg ttgctagatt
    31921 cattgaggtt cggcgaatta gataatccaa gaacgaaata tttactagaa gaagtagttg
    31981 caggtatcgg cggatacggc aattgtatcg gcataccgac tgtaggcggc gaaattgcat
    32041 ttgatccttg ttatgaagga aacccattag tcaatgcaat gtgcgtaggt ttgattgaac
    32101 acaaacatat ccagaaaggg caagccgctg gcgtcggtaa ttcgatcatg tatgtaggtg
    32161 caaaaacagg tcgcgacggt atccacggag caacttttgc ctcagaagaa tttaatcaag
    32221 aagaagaaca gcaacgttct gctgtacaag taggcgatcc ttttatggaa aagctgctgc
    32281 ttgaagcgtg tttggaatta atcttgcatc attcggattc tctaataggt atacaggata
    32341 tgggagcagc aggacttgtt tcatctagtg cggaaatggc ttcaaaagct ggctcaggac
    32401 ttatattgac attagatgaa gtcccacaaa gagaaactgg gatgacacct tatgaaatga
    32461 tgttatcgga atcacaagaa cggatgttga tttgtgttaa atcaggagag gaagaaaaaa
    32521 tccaagaact atttcagaag tatgatctag atgcagtcac tatcggtaaa gtaacagacg
    32581 acgggcaata tcggctatat catcaaggag aagaagtagc aaatctgcca gtcgatgcat
    32641 tagctgaaga tgcaccagtt tatcataaag aaatgaaaga accagctcga atcgtagaat
    32701 ttcagcagtt agctccttat cagccagtta tcgaagaacc tggacaagta ttgcttgatc
    32761 tgctgcagca accgacgatt gcttcaaaac gttcaatcta cgaaacttat gattcacaag
    32821 tacaaaccaa tacagttgtc cgtccaggaa gcgatgctgc tgtgatgagg gttagaggga
    32881 cgaataaagc gttagctatg accacagact gcaacgcgcg ctatctgtat ttggatccaa
    32941 aaatcggagg acaaatagca gtggcagaag ctgctagaaa tattattgct agcggtggta
    33001 agcccttggc gatcaccgac tgcctgaact atggttctcc tgataaacca gaagtttttt
    33061 gggaattatc gacttcagct gacggaattg ctgctgcgtg tgaaacgtta ggaactcctg
    33121 ttatttcagg aaatgtctct ttatataatg aaacggatgg tcaagcaatc tatccaacac
    33181 caatgattgg tatggtcgga ttgattgagg atcataaaca tatcactact caggaattca
    33241 aaaaatcagg ggatctaatc tatatattag gtaagacgtt tgcagatttt gatggcagtg
    33301 aattacaaaa gatgcagctt ggaagaatcg aaggtgtgat ccgaaacttt gatctatcca
    33361 ttgaaaagag aaaccaagag ttagtattga cagccatcca aaacggattg attgaaagtg
    33421 ctcatgactg ttcagaaggt ggcttggcaa ttgcactggc agaatcagct ttcaaacatc
    33481 agctagggat atctgtacag tttgaattgt cctctgctca attatttgca gaaactcaat
    33541 caagatttgt gttgacagtc gcaccggaaa ataagacacg attcgaagaa atgatgggag
    33601 atgctgctgt tttagcaggt aaggtgacgg atgaagcgat aatcgaaatt tcagcaactg
    33661 acggtcagat caaaatagaa actgcagtag caagaaagtg ctgggaggat gcaatcgtat
    33721 gtcttatgaa gtgaagagtt taaatgaaga atgcgggatc ttcggtgttt ggggtcatcc
    33781 agacgccgct agtgtggcat acttcgggct ccatagtctt cagcatcgag gacaagaagg
    33841 tgcaggtatc gtgtccaatg atcgaggaaa gcttaagggg catcgagatt tggggctgct
    33901 ttctgaggta ttcaaagatc aaagaaaatt gcagcagtta acaggagaag ctgcaatcgg
    33961 acatgtacgt tatgcaacag caggcaatgg aagtgtggac aatattcagc cttttctatt
    34021 caaatttcat gatcagtcga tcggcttagc gcataatggt aacttgatca atgctcgttc
    34081 ccttagaaaa gagttagagg aaaatggggc gatcttccat tctaactccg atacggagat
    34141 attgatgcat ttgatccgac gaagtgaaga aacagatttt ttaaataaat taaaaagtgc
    34201 tttgaatgaa gtcaaaggtg gctttgccta tttattaatg acagaaacgg caatgattgc
    34261 cgctttggac ccaaatggtt ttcgtccatt agcaatcgga caaatgaaaa acggagcata
    34321 cgtgattgcc tcggaaactt gtgcattaga agttattggg gcacgtttta taagagatgt
    34381 ccagcctggt gagatagtga tcatcaatga cgaaggaatc cagattgatt gctttacgaa
    34441 ggacacgcag ctttctattt gttcgatgga atatatctat tttgccagac cagattcaaa
    34501 catcgcaggg atcaatgtgc atactgcacg gaaaaatatg ggacggaatt tagcgaaaga
    34561 atcgccagta aaagcagata tggtcgttgg cgtacctaat tcttctttgt cagccgcaag
    34621 cggttatgcg gaagctagtg gaatccctta tgaaatcgga ttagtaaaaa atcaatacat
    34681 tgcccgaaca tttatccagc caacgcctga gctaagagag caaggtgttc gaatgaaatt
    34741 atctgctgta cgaggagtag tggaaggtaa aaaggtgatt atggtcgatg attcaattgt
    34801 tcggggtacg acaagcagac ggatcgtcca tcttttgaaa gaagcagggg caaaagaagt
    34861 ccatgtgagg attgcttctc ctcctttgaa atatccatgt ttttacggga ttgatattca
    34921 aacgcgtaaa gaactcattg cggcaaatca cacgattgat gaaatcaaag aatgtatcgg
    34981 tgcagattct ttaggttttt taagtgaaga gggtctgatc gaatcgatcg gtctggaaaa
    35041 agatgctccg tattcaggat tgtgtatggc ttatttcaac ggagattatc cgacgccgtt
    35101 atatgactat gaagaaaaat atcaggaatc actaaaagaa aaagtatcgt tcttttaaaa
    35161 gcagaccaag aagggagtat atgaattcaa tggcaaatgc atacgcaaaa gctggcgtag
    35221 atgtcgaagc tggatatgaa gtagtagaac ggatcaagaa gcatgtcaaa aaaacggaac
    35281 gtttaggtat gatgggcgct ataggaggat tcggcggttg ttttgattta tcggtctttc
    35341 aattaaaaga gccagtcctt gtctcgggga ccgatggcgt ggggacgaaa ttagcagtgg
    35401 caatcgaagc agacaaacac gatacaatcg ggatcgactg cgtagcgatg tgtgtcaatg
    35461 atatcttagc acaaggcgca gagccgttat atttcttaga ctacttagca acggggaaaa
    35521 atgatccgtt acgtttggaa aatgtagtaa aaggtgtagc agatggatgt gttcaaggaa
    35581 atatggcgct gattggcgga gaaacagctg aaatgcctgg tatgtactca ggtaatgatt
    35641 atgatctagc cggatttgct gtagggatag ctgaaaaatc tcaattattg acaggagaaa
    35701 cgatcaaccc aggagatgtg ttgcttggaa tagcatctag cgggattcat tcaaatgggt
    35761 actctcttgt tcgcaaaatt ttctttgagc agcagcagtt ttctttatca tcaaaactgc
    35821 cagaattgaa aaaaacactt ggcgaagaat tgcttgagcc aacgaaaatc tatgtttctt
    35881 ccttgcttcc gctgatcaaa gaaaagaaaa taaaaggagc tgcacatatc actggtggag
    35941 ggtttgtaga aaatatcccg cgaatgttac cagaatctat ggctgcacaa atcaaacttg
    36001 gctcatggcc gattcttcca atattcggct gtttacaaaa atatggtgaa attcctgaga
    36061 tggagatgta tgaaatcttc aatatgggca tcggtatggt cctggcagtc gaagaatcga
    36121 aagtaaaaga aatagtacac gaaattgagc aagctggcga aaaagtttat gaaatcggga
    36181 aagtgatcgc caaagaaaaa catcgtgtgg tgtttatagg agaagaacga tgagaatcgc
    36241 tgtttttgct tctggaaacg gaagcaactt tcaagcgcta gccgattatc tatcgaaaaa
    36301 aggattggaa tcttcgattg actggttgtt ttgtgatcag cctgaagcat atgttttgaa
    36361 acgtgcaact gcgttgagtg taccggcaga ttgtttttca cctaaagaat ttgattcaaa
    36421 aaaagagtat gaagaagcaa ttttgcataa attgaaagaa aaaaagatcg atttgatcgt
    36481 attagctgga tatatgcgaa tcattggacc ggtattgctg aaaaattatg ataaaagaat
    36541 catcaatatc catccgtctc ttttacctgc cttccctggt ttgcatggca tacgtgatgc
    36601 ctttgaagct ggagtaaaag aaacgggtgt cacaattcat tatatcgacc aaggtgtaga
    36661 tactggacca atcatccggc aggaaaaagt gagaattgaa caggaggaca cgttcgattc
    36721 tttagaagaa aaaatccata gagtagagca tcgaatctat ccagaagtga ttagtgaaat
    36781 tattgaaaat ggaggctatc tgaaatgaca agagcattga tcagtgtatc agataaaact
    36841 gggattgtag cattcgccca aggtttgatt gaacaaggag tagaaatcat ttcaacagga
    36901 ggaacaaagc atgcgttgga taaagctggg atcaaaacgc tttcgatcga agaagtgaca
    36961 gggtttcctg aaatgatgga tggccgtgtc aaaacacttc atcctaaaat ccatggtggt
    37021 cttttaggaa gacgcgatct ccctagccat atggaggcaa tggaaacgga aaatatccaa
    37081 ccaatcgact ttgtttgtgt caatttatat cctttcaaag aaacgattat gaaaccagat
    37141 gtcgaaatag cagaagctat tgaaaacatt gatatcggtg gtcctagtat gctccgtagt
    37201 gcagcaaaaa accatgaatt tgtcacctct gttgttgatc catctgacta tgaagaagta
    37261 ctttcagaat tacgtcagta tgggcatact tcactttctt tcagacaaaa actagcggca
    37321 aaagtctttc gtcatactgc ggcatatgat gcactgatcg ctcaatactt aacggattgg
    37381 gtggaagaaa aagaaccgga aaaattgaca cttacgtatg aacgaaaaca aacgcttcgt
    37441 tacggtgaaa atagccatca agccgctgcc ttttatcagt ccgctcttct ggaaaaatat
    37501 tcgatcgcag cagcaaaaca actccatggg aaagaacttt cttataataa tatccgagat
    37561 gcagatgctg cgctacggat catgaaggaa tatgacgagc caacggtagt cgccttgaaa
    37621 cacatgaatc cttgtggtat tgggacagca tctacgattt tagaagcgtg ggaagcagcg
    37681 tatgaagcag atcctgtttc aatctttggt gggatcatcg ttttgaatcg agaagtagat
    37741 ctgccaacag cagaacagat gcataagctg tttctagaaa tcatcattgc cccttcttat
    37801 acacaagaag ctttggaatt actgcaaaaa aagaaaaaca tccgattact gacagtagac
    37861 tttgcgcagg aagaaaatca aaatcgagaa gaaacggtct cagtcttagg tggacttctt
    37921 gtacaagatc aagatctggc aatagaaaat gaagaagagt ggaaagtcgt gacaaaacgt
    37981 aagccgactc cagcagaagc aaaagcatta gtgtttgctt ggagagctgt caaacatgtg
    38041 aaatctaatg cgatcgtact ggcaaacgaa cgtcagacag taggaatcgg agccgggcag
    38101 atgaatcgag tcggttcagt aaaaattgcg attgaacaag cgcaagagaa aatggaaggt
    38161 gcagttttag ctagcgatgc ttattttcca atggatgatt cggttgaata tgcagccaaa
    38221 catggtataa aagcaatcat tcagccagga ggaagcatca aagaccaaga ttctattgat
    38281 atggcgaata aatacgatct agcaatggtc tttactgatg ttcggcattt tagacattag
    38341 agaggagtcg gataaatgaa cattttggtg atcggtgcag gtggtcgtga acatgcaata
    38401 agtaaaaaac ttttagccag tccgaaggtg gatacggtgt attgtgcgcc aggaaatccg
    38461 gggatgacaa aagatggtat caattgtgtc gctatcagtg agcgagatca ttcagcgtta
    38521 atccattttg ttaagaaaga ggctattgat tggagtatcg ttggtcctga agttcctttg
    38581 ttaaatggga tcgtcgatga ttttatggcg gaaggattga aaatcttcgg tccgacaaaa
    38641 gcagcagctc tgatcgaagg ttcgaaaagt tttgctaaac aattaatgga tacttatcaa
    38701 atcccgacag ctaaatccag aagttttgaa tgttacgaaa aagcagcagc gtatatcaaa
    38761 gaaaatggtg cgccaatcgt agtgaaagcg gacggcttag cagctgggaa aggtgtagtc
    38821 gtggctacca cagtgaagga agcgctggat gcggcagaac aaatgttgaa ccagcatcga
    38881 tttggggata gtagcaaaaa aatcgtcgta gaagagtttt tggcaggaga agaattctcc
    38941 cttttggcgt ttgtccgtaa taccaaggtg tatccgatgg tcatttcgca agatcataag
    39001 cgggcatttg ataacgataa agggccaaat acaggcggaa tgggtgctta ttcaccagta
    39061 ccgcaaattt caaaaaagat gattgaaaaa gctgtggaag aagttttaaa accagcggct
    39121 ttgggaatga aagaactagg acgctctttc acgggtgttt tatatgcggg tttgattgct
    39181 acgaaggaag gaccaaaagt gatcgaattc aatgcgcgtt tcggtgatcc tgaaacgcaa
    39241 gtcgttcttt ctcggttgaa gagtgacttt gcgcaagtaa tcgatgattt attagagaat
    39301 cgtacagtag agttaaagtg gcagcaagaa ggatacaatg taggcgttgt ggttgcagct
    39361 gccggttacc ccgaagaata tgagacagga atggttctac cagattttct ggaagaagag
    39421 ctttctgttt actatgctgg agtttcctca aataacggca aactagttgg agcaggagga
    39481 cgtctttatt tagtagaagc atacggtgag acgatcaaag aagcacagaa aaaaatttac
    39541 accgcgttaa tggataaaac gacagatgga acattttatc gtacagatat tggagaaaaa
    39601 gctgccaata gcgaactgat cgtgaagtaa atcagctgct tccatttata aaaaatacaa
    39661 caaacataat cggaagcgcc gattttagtg aagccttttc ataaagagtg tggtacactt
    39721 ttgttgtgta tataaataag gaggagttca gatgaaaaaa attcaattag gtacttctga
    39781 tcagaaagca gctgcagtga tcttaggctg tatgcgtatc aacaatgcgg aagatccagt
    39841 aaaagtgatt gagacagctt atgaaaacgg cattgatttt tttgaccatg cagatattta
    39901 tggaaacggt gaatgtgaag aaatctttgc agatgctcta gcaaaaacat caatcaaacg
    39961 agaagatctt tttattcaaa caaaatgtgg aatcgttcca ggtgtgatgt ttgatttttc
    40021 taaaaaacat atcattgaat ctgtagaaaa aagtttgacc cgtctgaaaa tggattatgt
    40081 ggatgcttta cttcttcatc gtccagatac attggtagaa ccagaggaag tagctgaagc
    40141 gtttgatgaa ttggaacggt caggaaaagt tcgttatttt ggcgtcagca atcaaaaacc
    40201 aatgcaaatc gaattgctga aaaaatatgt tcgtcaacca ttagtagcta atcagctgca
    40261 atttggcttg aaacatactg gaatgatcga tcaagggatt catgtcaata tgacagatga
    40321 agcaagtatc gatcatgacg gtagtgtact tgattattcc cgattgcatg atatgacgat
    40381 ccaagcatgg tcaccatacc agtatggatt ttttgaaggt gtatttatcg ggaacgaaaa
    40441 attccctgaa ttaaaccaaa cattagaaga actagcagct aaatatcaaa cgacaccaac
    40501 tggcttggct tctgcttgga ttttacgcca tccagccaat atgcaagtga tcgccggtac
    40561 gatgaacgta gggcgaatca aggaaatcgc tcaagcttca gaaattaata tgtctcggga
    40621 agattggtac gaattgtacc gtgctgctgg aaacatttta ccttaaagat tgtcataagc
    40681 aggtcaaaag ggctataaat taaaaagaca gaataacttg aaagctgttt tcagcttcga
    40741 gatattctgt ctttttttat tgcaaatgga aagagtatca ttgtacggat ttcgtttatt
    40801 agttattttt gtttttaagg aaaagctgtg tccgatattc ttttggagaa atcccttgtt
    40861 ttttcttaaa taaataactg aaatagttag ggtcattata accaatttca aaagcaatat
    40921 ctgaaatacg atagtctgat tgctgcaata atttttttgc taattcgatt ctttgttctg
    40981 tcagataatc gatgaatgtt tggttaaacg attgagaaaa aatcgtactg aaatgagagg
    41041 gacttaatgc aacttgttga gcgacccaag ttaaggacat atctggatcg gtaaaatttt
    41101 ctttgataaa atccaatgca tggttcaaaa ttggctgata tttgttcctt gaaggctgtg
    41161 agggcagagc agttaagaaa gaaatgaaca tataaactgt cgaacggtaa agcgagtcgt
    41221 cggcgctgat tgctaagcgt tgactggtgg atgtgcaatg aaccatttct tgcgggattt
    41281 tcttttcttt caggcagaca tgctggagta gcttagtcaa gacaaataaa cgatataatc
    41341 gtgttcgagt tggtgtgcct tgtggtttcg tcaagtgttc gatgtactta gatacttcgt
    41401 ttttcgataa tgaagacaaa tcaagtgaga ggtcaaagta aaagacagaa tcaactggaa
    41461 gtttcgcttc ttgaatcaaa tcctcatagc tgataatttg ttctttaagg gaactggcgg
    41521 tttcaagtat catctgcgtg atctcaaaag atgcaggtaa ttcgctgatt ctttctgcga
    41581 caggaccaat ggcgacagaa aaattatttt tttgtccctt ttctaattca tggaccaacg
    41641 tttgggctac ctgataactg gtctcgatca cattttcttc aagagaatca actattaaaa
    41701 atttgatgaa tcgagaggaa acagcagaga agatcacttg ttgattctcc ctaaataaaa
    41761 acttcaaata atcactgaga tgagcgtaat cttcaaagtc tgtactgact tggtttgctg
    41821 ctaataatac ggtcattttt ttccctgcta aagagcgatt gaaagattca gcaagtttca
    41881 atgtttcact gatcgtaagt gagcctttga acaaaccgtt caagaaatga ttttttttca
    41941 attcgaaaac aagatcagaa gaagtgtgcg tttctttttg ctgcaaatct aaagcagtta
    42001 ctgctttttc tattgcttgt ttcaattcgc tacttttaat cggtttaagc agataatcat
    42061 ctgcctgtac ctggattgct cctttcatat aatcgaaatc gtcgaatcca ctaataaata
    42121 tgagacgagt ccaaggaagt agtttccttg cttcttttga aaaagtcaac ccgtccatga
    42181 aaggcatttg gatatcagtc agaatgatgt cgggctgtaa gtcgataatt gaagacagtg
    42241 ccatctcacc atctccagct tcgccgacat aagtgattgg ataataagaa ccaaggacag
    42301 ttatttgttc ccgcaagtta ttgcgaatga ggtattcgtc ttcgatgata aaaactttat
    42361 acatgtttgt ttcctccaat tgttttggga gatagaggga tcatcagtgt gacagttgtt
    42421 ccttctcggt atttgctgtc aatcgtgata ggtttttgat catttccgta ataaagaagg
    42481 agacgcttgt ttacgttgta aagtccgtag ccgatactga aatctgaatc gatccctttg
    42541 gctaactctt tacgtatttc tgccagtttt tctgcaggga tgccaatccc gttgtctata
    42601 actttgaatt ggatcatctc atcaattttg catgtggtaa tacgaataaa tcctttgtga
    42661 cggacgtact tcgttccatg gtatacagca ttttcaatca atggctgcaa gatcattttt
    42721 aaaatagaaa caggaaaaag tgtttctggt atatcgatct tatagtcaag catatggccg
    42781 tatcttattt ttaagatgac tagataatca ttgatatggc taatttcttt ttcaagagga
    42841 atccagtctt gtcctttgct taaagacgta cgaaaaaagc cggaaagtgc atgggtcata
    42901 gtcagagctt cttcaaagca gtcttgttcg atcaaagaaa taatggcatc caaactgtta
    42961 taaataaaat ggggagtaat ctgagcttgc aatacacgaa cttcactttg tgctaaatgg
    43021 tattgtttca aagcgttctc ccgtaaagac tggtttagat cttccgccat tttattcatg
    43081 ctagcggtaa gtgtttgtaa ttccagtgtt tccggcaggg ttaaacgaaa gccaaggttc
    43141 cctgaagaaa gttgttctga catctcgatc agctgattga tcggttcttg gacctttctc
    43201 tctaaaaagc gacgattctt tttcgcatag aaaaaaatgg tactaagaac tccaagttcc
    43261 actattgtta atagaatgag cgagcggaca agtgtcgtat tttttttgct tgctaagtcg
    43321 atctccacac gaacaaaatc ttgtaagata tctgacagca gttgagtgac agaatctacc
    43381 tgattgatga tctcttcatt ggtatccata aaatcttttg ccaagatatt attgatcagc
    43441 tcgttttgat aatcttccag tgtatcgatc gtacgtagcg atacatctaa cgtactaatt
    43501 tctttaagtg tatttgtatt atttttgctc tctcgaatat cattcctaag ttcttctagg
    43561 atattttcct cattgaactc atctacggtt acttggccag aaatcaagtc ccacatactt
    43621 tccaaaactt tttgatcaac cttcaaagca attgcatctg ctttttggat atttgcgatc
    43681 gtctgttgat aaatcaaaag ctgttgcgaa tataacagta tgctcagtag caaagggata
    43741 acagcaaata gaagcatggc tttgtttgct tgggcaaaaa tctgttttat gcttaaattt
    43801 tttgtcatga tacttctcca gtcaataatt tctggttggg atgttagcta tattctgctg
    43861 ggaaaagaca gtctcggaaa taaaaatatc tttaggtaat gtttttcctg tttgaatatt
    43921 tccaaaataa cgagtaacgg tattggctac ataccagccg acatcaggtt tacactcgac
    43981 tacacaattg acgattcctg agcgtagctt ctcgatcata tcttcttgtc catcaatagt
    44041 tacaatgatc aaatcttctc caggtttgat tgatgtttct tttatcgcag ctaaagcacc
    44101 gtgagtcatt tcatcactat ggctgtataa tacatcgatt tcggataaat catttgtttc
    44161 taaatatcgt ttcatctggt cttttccttt catccgaata aaatcaccga caattcggtg
    44221 aacaatagtc atcggaggtt tatggtcgat ggttccatct ttttttaggg ttggtttacg
    44281 attgattgat tccaaaaatc cttcggagcg tagcttggtc ggtgtggagt tctctgaacc
    44341 agcaagttct aggattctga tttcctgttt ctttgaattt gtgaagtgat tgctgacaaa
    44401 taaacccgcc cgatttcctt ctgctttgaa actggggcca atgtgggtga cgaaaagctc
    44461 gggatcattc gtttgaatat gtctgtcgac aagtatgaca gggatccctg ctcgctttgc
    44521 ttcttttaat acagagtccc acccggattc ttgcaaagga gtaaaaatga tcatatccac
    44581 tttataggca atgaacgtcc gtatatcttg gatttgccgt tcctgattca tgtagccatt
    44641 tcggtagaga acttggtaat cttctttttc aagcgcagtg ataattgatt cagtatgctt
    44701 tttccgccag ctactctccg ttcctgattg cgaaaaacca acgactagtg ggtcaggtgc
    44761 ttcctttgta ttaggtgtac accccatcat ttggtaagcg attacaacaa gggctaaaaa
    44821 aaataaaaca cgtttcactt taaatcactc catctggatt tgttagtact atcttatcag
    44881 ataaaaggga aaatcaaagt caaaaggaat caatcgaaaa aaatccaaga aacttaaaaa
    44941 aatccaagac aaaaaaagta cacctaaaaa atttaaagta aatcccataa aaaaatcaat
    45001 gttaacgctt tctttattag ctatactcgg tgtatcaatt gaaatggggg ataaaaaatg
    45061 aaaaacaaat ttagaaagat tgttgggact atcgctattt tgaccgcaag cattctatta
    45121 agtgcgtgtg gcaattcagg atctgcagat gatagtacgg ggtatgtagg aatcgctatg
    45181 ccgaccaaat cagcagaaag atggatcgct gatgggaaca acatggtctc ggaattagaa
    45241 aaactgggtt ataaaacaga tcttcaatat ggagaagata aagtagaaaa tcaagtagca
    45301 caaatagaaa atatgattac aaaaggggta gatacacttg tgattgcttc tatcgatggt
    45361 tcggctttaa ctgatgtgtt agctaaagca aaagaagcag atatcaaggt gatcgcctat
    45421 gatcgtttac tgatgaattc agaaaatgtc gattattacg cgacttttga taattttgga
    45481 gtaggtgtat cgcaagcagc ttatattgaa gagcacttag gattgaaaga aggaaaagga
    45541 ccattcacga ttgaattatt tggtggctcg cctgacgaca acaacgcact aatcaattac
    45601 aacggagtga tgtctgtttt acaaccatat atggacaacg gtcagttggt cgtgccatct
    45661 ggacagacga gtttcagtca aatcgctacg cttcgctggg atggttctac ggcgcaagca
    45721 aggatggata atctgttaag cgcaaattat actgaccaaa cattggatgc tgttttgtcg
    45781 ccatatgatc caataagttt aggaatcatt tcgtcattaa aaggcgtggg gtatgggagt
    45841 gaaagtaaac ctttacctgt gattactgga caagatgcga cagttgctgg cgttaaatcg
    45901 atcattgccg gggaacaaac acaaacgatc tttaaagaca cacgtatctt agcaaaaaat
    45961 acgattgaaa tgatcaaagc aatcagtgat ggagaagagg ttccggtcaa tgatactgaa
    46021 acttatgata atggggtgaa aactgtccct acgtatttag caaatacagt atctgtcgat
    46081 aaagataatt atcaagcaga gctgatcgac accgattatt ataaagaatc cgatctgaaa
    46141 aattaagagg aaccaaacaa ggaactaaag agatctaagt cagagagagg ataagaatta
    46201 tggcggattg tattttaaaa atggcagata tcaccaaaaa attttctgga atccgagcgt
    46261 tagattgcgt gaatctgatg attgaacgtg gggaaatcca tgcattgtgc ggagaaaacg
    46321 gagcgggaaa atcaacattg atgaatgtct tgagcggctt gtatccatat ggctcctatg
    46381 acggggaaat cttttatgat ggaaagctgt gtcaatttaa agatttgaaa gatagtgaag
    46441 aaaaaggcat cgtcatcatt catcaagaat tagcactaag tccttatctt tcaataaaag
    46501 agaatatttt tctcggaaat gaacaagcag aaagaggcgt gattgattgg gaactgacag
    46561 aaaagaaaac atatgaactg ctgaataaag tcggtttgtc tatcgacccg aatcttctag
    46621 tatcccagat tggcgtaggg caacagcaac tagttgaaat agctaaagca ttatcaaaat
    46681 ccgtccgtct gttgattttg gatgagccta ccgctgcatt gaatgaagaa gaaagcgcga
    46741 atttgctgag attgatccga gagttgaaag agcaaggtgt aacctccatc attatttcac
    46801 ataagctgaa tgaaatcgtg gatatcgcag atcgaatcac cattttacgg gatggacaga
    46861 cgattgagac actagaaaat gaaatgatca gcgaagagca tattattcgc ggaatggtgg
    46921 gcagagattt agttgatcgt tatcctgaaa gaaaaccgga tattggagaa gtttatttta
    46981 aagtagaaga ctggacggtc tatcaccctc ttgatcacga gcgaatcata aacgaccaca
    47041 tcaacttgca tttgaaaaaa ggagagattg taggaatagc tggactgatg ggagcgggca
    47101 gaactgaatt cgctatgagt ctatttggac attcttatgg aagcaagata tccggacgta
    47161 tattaaagga aggggcagag atatcagtta agaccgttcc ttcagccatt gcaaacggac
    47221 tcgcttatgt ctcggaggac cgaaaatctt taggactgaa tctgctgatg gatattcggg
    47281 aaaacgtgac gctgagcagt ttggataaaa taagtcaaaa aggagtatta gataaagaaa
    47341 aagaagtaac tgaggcagaa aatttccgga aaaagatgcg tataaaaaca aactccatcc
    47401 atcaaattgt cagtagtctt agtggtggga atcaacaaaa agtcgtttta tccaagtggc
    47461 tgatgacaca acccgatatt ctttttttgg atgagccaac ccgtgggata gatgttgggg
    47521 caaaatatga gatctacacg attatcgaag aaatggcagc aaatggcaaa gctgtctgtc
    47581 ttatctcttc tgaacttcct gaaatattag ggatgtgtga tcgaatctat acgatgaacg
    47641 aaggacgtat tacaggagaa gtctcgcgag aagatgcaga tcaggaagtc ttgatgaaat
    47701 taatgacgaa agaagaggtg ggttaagttg gaacaggcaa aaaatcttac ggataaaaaa
    47761 gagaaaaaac taaatttgaa aagtagagca gctgatcttt ttggtcgcta cagcatggtg
    47821 atcattttag cgggactact tattgcgttt caattgatga cggatggtat tttctggcgt
    47881 ccattgaata taactaacct tgttctgcaa aatagccata tattagtact agctgcaggg
    47941 atgttattag ttgtgctctt aggtcatgtg gatttgtcgg tagggtcagt gatggcgttt
    48001 gttggtgcaa ttgcgggcat gatgatggtc aatcgccaaa tgaacccgtg gatgacagtt
    48061 atcgcatgtc ttgcagtagg cgggctgatc ggcgcatggc aaggtttttg ggtagcttat
    48121 gcagggatac cggcgtttat cgtgacttta gcaggattgc tgatgtttcg tggactgaca
    48181 caagttgttc taggaggcca atctctagca ccttttccag ctgttttcca aaaaatgtct
    48241 acgggttatt taccagactt aagcgaagga agcagccttc acttcttgac attgatttta
    48301 ggaatgatca tcacgatcgt gttagttgtt ggtcaatggc gaacaagaaa tcgtcggaaa
    48361 aatcatttat ttgaagtgga atctccaaca gctttcttgg taaaggcggt atttacagca
    48421 gtcgttgtac taggaataac ttatatcttt gcctcttatc aagggtatcc gattattttg
    48481 atcattcttg gtttgatcgt tgcagcatat gggtttttga caaataaaac agtagcaggt
    48541 cgacagattt atgcgacggg cggaaatcga aaagcggctg aactatcagg gatcaaaaca
    48601 aagcgaatca cgttctgggt attcgtcaac atgggagtca tggcagcttt agctggatta
    48661 gttttagctg ctcgattgaa tgcggcaaca cctcaagcag gtacatcgat ggaactggat
    48721 gcaatggcgg cagtatattt tggtggtgct tcgacttccg gcggcattgg aacaatcatg
    48781 ggtgcgattg tcggtggctt ggtcatgggt gtattgaaca atggcatgtc gattttaggc
    48841 gtcggggtag actggcagca ggcaatcaaa ggattgatct tgcttttagc cgttgtgcta
    48901 gacttgtata ataagaaaaa acgaacaacg taggagaacg ccggttattc agaaaaaaga
    48961 ctgtgacaag acatttgtca cagtcttttt ttcgctaaaa gcgaacattc attgtttcgt
    49021 ttgtatagac taaaaataat atatgttttt ttaccattcg tataaattct ctttttgctc
    49081 ttgatttttt tgcttaattg agttaaatta catagggcgc taaaaaaagc cttgattaaa
    49141 ttaataatgt tcgtgttttg ctggatgtga acatcaggag gataatgtaa tgaaagtatt
    49201 tgattacgaa gatattcaat taattcctaa taaatgtatc gtaaatagcc gttctgaatg
    49261 tgatacaacg gtcacattag gtaaacatac attcaaaatg ccagtagtgc ctgccaacat
    49321 gcagacgatt attgatgaaa caatcgcaga attcttagcg gaaaatggtt atttctatat
    49381 catgcatcgg tttgatgaag cagctcgaat tccgtttatc aaaaaaatga aaaagcgtgg
    49441 attgattacc tcgatcagtg ttggcgtcaa gaaagaggaa tattccttta tagagaagtt
    49501 ggcagaagaa tcgctaaatc ctgattatat cactatcgac atcgcgcatg gacatgctaa
    49561 ctcagttatt gatatgattc agcatattaa aaaatatttg cctgaaactt ttgtgattgc
    49621 cggtaatgtt ggtacaccgg aagccgtccg tgaattggaa aatgcgggcg cagatgcaac
    49681 aaaagtcggg attggtccag gaaaagtatg tattacaaaa atcaagacag gctttgggac
    49741 tggaggctgg caactggcag ctcttagatg gtgtgcgaaa gcagctcgca agccgatcat
    49801 cgctgacggt gggatcagaa cacatggcga tattgcaaaa tctgtccgtt ttggtgcgac
    49861 aatggtcatg attggttcat tgtttgccgg tcacgaggaa tctcctggcg aaacaaaagt
    49921 cgaaaatgga atcgtgtata aagaatactt tggttctgct tcagaattcc aaaaaggcga
    49981 aaaacgaaat gttgaaggaa agaaaatctg gatccaacac aaaggatctt tgaaagatac
    50041 actagtggaa atgcaacaag accttcaatc atctatttct tatgctggag gaagagatct
    50101 ggaagccatt cgaaaagttg actatgtcat cgtgaaaaat tcgattttta atggagacac
    50161 aatctaaaaa ataaataaaa tattaggatg ttgtgaaaaa gttgtcctgc attaagtaat
    50221 aagaacagcc aaacaaaaat agttctttat atgttttgtt tggctggaat tattatcgca
    50281 gatacaagct tttgaacatc attattcaga ataagggact gtgataagat ttttgtcaca
    50341 gtcccttatg ttttgcaaaa aaagtttttc ttttttctcg taaattcggt aaaatcaaaa
    50401 gaaaaaatag acaatagaaa agagaagctt atgtgggaaa aagtagagaa aaaacaagga
    50461 ccgatttatc gtcaaattat ggaacagatt atgagagaaa tcgaaaacgg aaggatcaat
    50521 cctggaagtc aacttgaatc agaacgaaaa ctagggcaac tttttggtgt gaatcgttcc
    50581 accgttgtcc atgcattgga agagcttagg gagcttgggg tactaacgag caaacgtggc
    50641 agcggatggt ttgttaatca gactgaatgg gggaaattcg ctgttccaag aatcgattgg
    50701 cggcaaatga tctctcctcg ttatgagcag acggactggt atgagcaaaa aataagagaa
    50761 gcaaagcaga tccaaaaaac atcgtttctt gatttatacg ctagcgagat gccagatgag
    50821 cttttgccga attttgagtt tccttcttat tctcttgaag aaatcttagc ggaagaaaaa
    50881 gaaatggatc ttttaggcta tactccgtta cgtaacaaaa tcaaacggta tttggaaagt
    50941 aaatttgaat tggcattttc ccccaaccag cttcttgtga ctggtggcgg tcagcaagca
    51001 atctttttga ttttacaaac gattctttca gctggagagg ggatagctgt agagtcacct
    51061 tctttttttt accgattacc tcttttcaaa gcttcaggaa cgcgtttatt tgggataccg
    51121 atggatgaag aagggatcga cttagaaaaa ctgaaagaat cgatccataa aaataaattg
    51181 aaagcagtgc ttatcaaccc gaattttcaa aaccctacag gaaaagtgat gagtcaaaaa
    51241 cgacgagaaa agctagtaga attgtgtcga cagtatcggc ttccaattat tgaagacgat
    51301 gtatttacgg atctttcatt tctaaaccaa gaagaagtcc ttcctatacg aacgattgat
    51361 ccggataatg tgttatacgt cggttcgtta tctagggttt taggaaaaac aacgaaaatc
    51421 ggttgggtga taggacctga gacttttatc tcgcaactag ctcaagcgca agaaatgatg
    51481 gagttttcga tgaatatcct tacacaaatc acagtggcta atgtatttaa tgaagcaatt
    51541 gatttgaaaa tgcaagatgt aagaaaaaag ttgaaagaaa aaagccgact gataagcgct
    51601 tggggagatt cacaatcgtt tttcaagctt cattcaattt taggtggtta ctatgcctgg
    51661 attacttggg atgggaaaaa actagacccc gaactagcag aaaaagtagt agatatgggt
    51721 cttggaattg caccaagtct actttttggg caagatataa atggaatcag aatcaattac
    51781 agtaggctgg atgaaaacca aataccgttg ttttattcaa agatgaaaat tctagaaaag
    51841 tggttaacag aataatttca caaagtaaat agattaaaga atataacagc ttggatttaa
    51901 atttcaagct gtttcttttt tttcggaaaa agaatacgat ataaagaaac taagaaaaaa
    51961 ccattatttg tacgtccgtt cacgatgtgt aaataaaata aatgtttgca acaaaactgt
    52021 ataacaccaa cgtttttaaa tagaaacaga tggttgtaag tttgattaga aaatagaaaa
    52081 aatgcagatg aaatataaaa cgctttcctt attgtgattt tattcactaa tgctgttaat
    52141 atgcggattg gcataatttc ctttcatcgt gaaaatttgt gcttttagct tgtatttctc
    52201 ataaatccat gataatttat taatgaagac ggttaaatag tgatacagtt ttattcactg
    52261 tttcaatcgc agaatgaaga gattttctta tagatgagag aggaatgtgt gacaaatggc
    52321 aaacaaaaaa gcaccgatcg acttccaagc actgctagat gaagtcaatg cagatttccc
    52381 agtatatcaa gcacttgatc aagatggaaa cgtagttaac aaagatttag tacctgattt
    52441 atccgatgat gaactcgttg aattaatgac tcatatggta tggtctcgtg tattagacca
    52501 acgttcaaca gcgctgaacc gccaaggacg cctaggcttt tttgcaccaa ctgctggaca
    52561 agaggcaagt cagctggcta gtgcttacgc atttgataaa gaagatgttc ttcttccagg
    52621 ttatcgggat gtacctcaac tagtaaaaca tggtttacct ttatctcaag cttttttatg
    52681 gtctcgagga catgctgcag gtaattctta tccggaagaa ttgaaagctc taccaccaca
    52741 aattatcatc ggtgcacaat atgtacaagc agccggtgtt gcattaggtt tgaagaaacg
    52801 aaacaagaaa aatgtcgtct tcacttatac tggtgatggc ggttcatcac aaggtgattt
    52861 ttacgaagca atcaatttcg ctggcgctta ccatgcaaat gccgtatttt atatccaaaa
    52921 caacgggtat gcgatctcta cacctcgtga aaaacaaact gcagcaaaaa cacttgcaca
    52981 aaaagctgtc gcagctggga ttcctggtat tcaagtcgat ggaatggatc cgcttgcagt
    53041 atatactgta tctaaaatgg cacgggaatg ggcgatcagt ggaaatggac cagtcctaat
    53101 cgaaacactg acttatcgtt atggaccaca tacactatct ggtgacgatc caacccgcta
    53161 ccgtacaaaa gacacagatg acgattggca gaaaaaagat ccattgatcc gcttccgtaa
    53221 atatttgacg gaaaaaggac tttggtctga agaaaaagaa gaacaagtca tcgaagcaac
    53281 taaagaagaa atcaaagcag ctattgctga agccgacaaa gtaccaaaac aaaaggtttc
    53341 agatttcttg aaaaacatgt tcgaagtatc tccacaatca atcaaagaac aaattgcaat
    53401 ttatgaagcg aaggagtcga aataatcatg gcacaaaaaa caatgatcca agcaatcaca
    53461 gatgccttag cacttgaact agaaaatgac gagaatgtcg ttgtttttgg tgaagatgtt
    53521 gggaaaaacg gaggagtttt ccgggcaact gaaggattgc aggaaaagtt cggagaagac
    53581 cgagtattcg atactccttt agctgaatct ggaatcgcag gattgagttt cggtcttgcg
    53641 ttagaaggat ttcgtccagt tcctgaaatc caattctttg gttttatttt cgaagcaatg
    53701 gacgaagtag tagctcaaat ggctcgtaca agatatcgaa tgagcggcac tagaaatcta
    53761 cccatcacaa ttcgttcgcc atttggtggc ggtgttcata caccagaatt gcattcggat
    53821 aatttagaag gtttgatcgc tcagtctcct ggtattcgag tagtgattcc ttccaaccct
    53881 tatgatgcaa aaggactatt gatttcttct attagaagta acgatccggt tgttttccta
    53941 gaacacatga aactatatcg ctcattccga gaagaagtgc cggatgaagc ttacgaagtt
    54001 cctttggata aagcagcagt tactcgtgag ggaacagatg tatctattat cacatacgga
    54061 gcaatggtgc gtgaagcaat caaagcagca gacaatcttg caaaagaaaa tatatctgtt
    54121 gaaatcattg atcttcgtac agttgcgcca ttagatgtag aaacgatcat tcaatccgtg
    54181 gaaaaaactg gtcgcgtagt tgttgtccaa gaagcacaac gacaagcggg cgttgccgcg
    54241 caagtagttt ctgagatctc agaacgcgct atcctttcat tagaagcccc aatcggacga
    54301 gtttctgcac ctgatacagt cttcccattt ggacaagcag aaaatgtctg gttgccaaat
    54361 gcaaaagata tcgaagacaa agtaaaagaa atcgcggaat tttaagaaaa gaagggacga
    54421 aacaaagtca tggcttatca atttaaattg ccggatatcg gtgaaggaat cgcagaaggt
    54481 gaaatcgtca aatggttcgt caaaccagga gatacgatca atgaagatga tacattacta
    54541 gaagttcaaa atgacaaatc agtagaagaa atcccgtcac ctgttacagg aacggttaaa
    54601 aatgtgattg ttccagaagg taccgtagca aatgttgggg atgtattagt agaaatcgat
    54661 gcaccaggac acgaagacaa tgaaggggac agtggtgtag ccgcagaatc ccaaactcca
    54721 gctaaaccgg ctgctgaacc cactgttgat acagaatcag ctggatcgtc aagcgaagga
    54781 gtcttccaat ttaagttacc ggatatcggt gaaggaatcg cagaaggtga aatcgtcaaa
    54841 tggttcgtca aaccaggaga tacgatcaat gaagatgata cattactaga agttcaaaat
    54901 gacaaatcag tagaagaaat tccgtcacct gtcacaggga cagttaaaaa cgtgattgtt
    54961 ccagaaggca ctgtagcaaa tgttggggat gtattagtag aaatcgatgc tcctggacac
    55021 aatagtgcac cgtcaactag tgcgccatca gctgaagcac caaaagaaaa agtggaaaca
    55081 tcaggttcag caagtgtcgt tgaagctgcc gatccaaata aacgtgtatt ggctatgcca
    55141 tctgttcgtc aatttgcccg tgaaaaagat gtagatatct cacaagtaac tgcaactgga
    55201 aaaggtggca gagtaacaaa agaggacatc gaaaacttct tggcaggtgg tccaagcagt
    55261 gcaccagcga aatcagaagc gccagaagcg gcagcaccga aagaagcagc tcctgcagca
    55321 gaaagcaaac cagctgcacc agcaaaacca ttcaagtcca acttaggaga cttggaagaa
    55381 cgtgtagcaa tgacgccaac acgtaaagct atcgctaaag caatggtcaa cagtaagcat
    55441 acagctccac acgtgacatt gcatgatgaa gtagaagtgt ctaaactatg ggataatcgg
    55501 aaacgattta aagaagttgc tgctgcaaat ggtacgaaac ttactttctt gccatatgtc
    55561 gtgaaagctt taacagcgac tgttaagaaa tacccagtat tgaatgcatc tatcgacgat
    55621 gcaaaccaag aaatcgttta taaacattac tacaatatcg gtattgcaac tgatacggat
    55681 cacggactat atgtaccaaa tgtgaaagat gcagaccgca aaggaatgtt tgctattgca
    55741 gacgaaatca atgagaaagc aaaattagct catgacggca aactatctgc agaagatatg
    55801 cgcaatggaa caattacgat cagcaatatc ggatctgtag gtggcggttg gtttacacca
    55861 gtcatcaact atccagaagt agctatttta ggtgtaggaa cgattgcaca acaaccaatc
    55921 gtaaatgctg aaggcgaaat cgttgtcggt cgtgtgatga aactgtcatt aagtttcgat
    55981 caccgtatcg tagatggagc aactgcgcaa caagcaatga ataatatcaa acgtctttta
    56041 gctgatccag agctattaat gatggaagga tgataaacaa atggtagttg gagattttgc
    56101 tgtagaatta gatacagttg taattggtgc gggacctggt ggctatgtag ctgccatccg
    56161 cgctgctgaa atgggacaaa aagtagcaat cattgaaaga gagtatattg gcggtgtttg
    56221 tttgaatgtc ggctgtattc cttcaaaagc actgatagct gcaggacacc attatcaaga
    56281 gtcactagat tctgaaatgt ttggagtaac tagtgaaaac gtcaaactag attttgcaaa
    56341 aacacaagaa tggaaagaaa acaaagttgt ccatacgttg acttctggtg ttggtttcct
    56401 attaaaaaaa cataaggttg aaacaatcga aggagaagcg ttcttcgtag atgatcacac
    56461 actacgcgtc attcatccag attcagctca aacttattcg ttcaataacg caatcatcgc
    56521 aacaggttcc cgtccaatcg agattccagg atttaaattc ggaggacgtg tgttagattc
    56581 tactggcgga ttagcattga aagaagtacc taagaaattt gtgatcatcg gtggcggtgt
    56641 gattggtgcc gaattgggcg gagcgtacgc aaacttagga gcagaagtaa cgattcttga
    56701 aggctcacca caaattttac caacttacga aaaagatcta gtaaaattag tagaagatga
    56761 tttcaagaaa aaaggcgtaa cagtcgtaac gaatgcaatg gcgaaagaag cagtcgataa
    56821 cggtgacagc gtgacagtga aatatgcagt ggacgggaaa gaagaatccg ttactgctga
    56881 ttatgtgatg gtcactgtag gtcgtcgtcc gaatacagat gatatgggct tagaacaagc
    56941 gggtgtcgaa gtaggagaac gaggattgat cacggtggat aaacaaggac gtacgaacgt
    57001 acctaacatc tatgccatcg gtgatatcgt tccaggggcg gcattagcac acaaagccag
    57061 ttatgaagca aaaatcgctg ctgaagcaat ctctggcaaa aaagtagctg tagattataa
    57121 agcaatgccg gcagtagcct tcactgatcc tgaattggct tctgtaggta tgacgatcaa
    57181 agaagcaaaa gatgctggta tcgaagcaaa agcatataaa ttcccatttt cagggaacgg
    57241 tcgggcatta tcgcttggta aaacagaagg attcatccgt ctagttacga cgatcgaaga
    57301 taacgtgctt attggcgcgc aaatcggtgg agtcggcgca agtgatatgg tctctgaatt
    57361 agctttagca atcgaatcag gaatgaatgc tgaagatatc gccttgacga ttcatcctca
    57421 tccatctctt ggcgaaatcg tcatggatgc ttcggaatta gcattaggtt taccaatcca
    57481 tatttgattt gaataataca ggcgaaacgc aactgaaaaa tcagttgcgt tttgtttttt
    57541 agaattatca ataatattat tatgtaaact taaaaatatg acaacttctt ggtttattac
    57601 cacttcctat gctacactga agaggaatat gaaataggag agttgaagat gcaagaatat
    57661 cagtatccac ttgatttaga ttggacaaca gacgaaatgg tcattgtaat gaagatgtgg
    57721 gaagatctgg aacgtgcgaa tgaaaaaggg ataaacaatg aagctttttt gaagacttat
    57781 cagcagttta aaactgttgt caaatccatt ggtgaggaaa aacgattggg acgggaattt
    57841 gaaaaagctt ccggttattc tttatatcgt acagtgcagc aagcaaaaaa aaatccaaac
    57901 aaaactttga gaatgaacgg gtgagataaa tggaacagat gattgatcat atcaagtcct
    57961 ggcttgaaga agctggtgag aaaatcaaat cttctcaaca aacattaaca atctcgcaaa
    58021 aatcaaatcg aacggatcta gtaacaaatg tcgatcgaaa catccagaat tttctaatcg
    58081 aaaaaatcca ttcctttgat ccagaggcga aaatattggc agaagaagaa gggtgtaatc
    58141 aattatcgga tctcgatgga agagtcttta tcatcgatcc tattgatggc accttgaatt
    58201 ttgtgattga aggggaaaat ttttgtatta tgctcgctgt ttatgaatcc ggcgttggaa
    58261 agcttggttt catttatgac gtgatgagaa atgaactttt ttggggtgga aaaggaatag
    58321 gcgtgtataa aaatgatgag ccattgccta gaccagcaaa taaatcctta tcggaaggct
    58381 tacttggtgt gaatagttat cttttcggtc ataataaata tggaatccga gaaattgcgg
    58441 aaagcagcat ggggacacgt atgtacggat gcgctggtct tgaactagtg gcaatcttga
    58501 aaggaaacca cattgcttat atttcaaacc ttagcccttg ggattatgca gcgggaaatg
    58561 tcttattgga agagtttggt atggtaacaa gcggtctttt tggtactcct ttgacatttg
    58621 aaaaaaggga atactattta gcagggactt cttgtgcata tgcagaaatc gcagaccttt
    58681 tgaaaaaaga aggctgaaaa aaaggtagaa atcattcaca aaaatgataa aaacgagcaa
    58741 aagattaatt ttctcgaaaa aatgaaaaca ttttactata tttcatgaaa aaagtctgct
    58801 ataattagca gtcgagtaaa atttgccctg tcataaagaa ccaataaaaa aggagttagt
    58861 tacattgaat tatagaaacg atatccgtaa cgtcgcaatt attgcccacg ttgaccacgg
    58921 aaaaacaaca ttagttgatg aactattaaa acaatcagat acattagacg cacatacgca
    58981 attgcaagaa cgtgcgatgg actcaaatgc gctagaaaaa gaacgtggta tcacgatttt
    59041 agccaaaaac acagctgtag actataaagg tattcgcgta aacatcatgg atactccagg
    59101 acacgcggac ttcggtggcg aagtagaacg gatcatgaaa atggttgacg gcgttgtgtt
    59161 ggtcgtagat gcgtatgaag gaacaatgcc tcaaacacgt tttgtattga aaaaagcact
    59221 ggaacaacat atcacaccaa tcgttgtcgt aaacaagatt gacaaacctt ctgctcgtcc
    59281 tgagcatgtc gtagatgaag ttcttgaact attcatcgaa ttaggagcag atgatgatca
    59341 gttagacttt cctgtgattt atgcatctgc actaaatggt acgtcaagct tgtcagatga
    59401 tccagcagat caagaaccaa ccatggcacc tatttttgat acaattattg aaaaaatccc
    59461 tgctcctgtc gataacagcg acgaaccatt acaattccaa gtttcattac ttgactacaa
    59521 cgattatgta ggtcgtatcg gaatcggacg tgtatttaga ggaacaatca aagtaggaga
    59581 ccaagtagca ttaattaaat tagacggaac agttaagaaa ttccgtgtaa cgaaattatt
    59641 tggtttcttt ggactaaaac gtctagagat tcaagaagca aaagctggag acttgattgc
    59701 tgtctcaggt atggaagata tctttgtcgg tgaaacagtt actcctgtgg atcatcaaga
    59761 tgcgttacct atccttcaca ttgacgaacc aacattgcaa atgactttct tagttaataa
    59821 ttcaccattt gccggtcgag aaggtaaatt cgtaaccgct cgtaagattg aagaacgttt
    59881 gatggcagaa cttcaaacag atgtatcctt acgagttgaa ccaacaaact ctccagatgc
    59941 atggacagtt tctggtcgtg gagaattgca tttgtctatc ttgattgaaa acatgcgccg
    60001 tgagggatac gaattgcaag tatctcgtcc agaagttatt gaaaaagaaa tcgacggtgt
    60061 aaaatgcgaa ccgtttgaac gcgtgcaaat cgatacacca gaagaatata tgggtagtgt
    60121 tatcgagtcc ttgagcttac gtaaaggtga aatgcaagat atggtccata caggaaacgg
    60181 acaaatccgt ttgacattct taacacctgc ccgcggtttg attggttatt caactgaatt
    60241 cctttcaatg acacgtggtt acggaatcat gaaccatact tttgaccaat atctgccaat
    60301 gcttcctggt caaattggcg gacgtcacca aggggcgctt gtttcgatcg atacaggaaa
    60361 agcaacgact tattctatca tgagtatcga agaacgcggt acagtcttcg tagaaccagg
    60421 tacagaagta tatgaaggaa tgattatcgg ggaaaacagc cgtgataatg atttgactgt
    60481 caacatcacg aaagcaaaac aaatgacaaa cgtacggtct gctactaaag accaaacatc
    60541 tgtcatcaaa aaaccaaaac aattgacgtt ggaagaatct ttggaattct tgaatgatga
    60601 tgaatattgc gaagtaacac cagaaagcat tcgcttgaga aaacaaattt tagagaaaaa
    60661 tgctcgtgaa aaagcatcta aaaagaaaaa ataatctgta aaaggatgaa gagagaaacc
    60721 agcttgttat aaaaaatagc tggtttcttt ttttgtccaa ctcttttctg tggagaagtc
    60781 atctttgtca atggacataa attcagaaaa tacgccgaaa gacgtttaaa agacttttaa
    60841 aaaacttatt tctagagagt ttttgtttca taataagata atgtttcacg aaaggagatc
    60901 aacttgtcct tcgaagaaaa tcgaagtaaa atggcaagag aaaggagcgg gaagatgaac
    60961 gacttattaa aacaatactt tggctatgat gagtttagac ctggtcaaaa agaaattatc
    61021 cagaaagtca ttgatcagga aaatgtatta ggaatcatgc ctaccggaag tgggaaatca
    61081 atctgttacc agcttccagc tttactatta gatgggctta ctgtggttgt gtccccttta
    61141 atctctttga tgaaggatca ggtggacgcg gcgaatcaat taggtatacc cgccactttt
    61201 atcaatagct cgttagatgg ttacgaaaca gctagacgct tccaagaaat cgatagacag
    61261 cagtaccgtt tactttatat cgctcctgaa cgttttatca tgccggattt tatccaagca
    61321 atgaagcgat ggaacgtccg tatgatcgct attgacgagg cacattgtat ctctcagtgg
    61381 gggcatgatt tccgcccaag ttatttacag atggcgaacc agctagatca actgccaaat
    61441 cgtcctgtaa ttgttgcttt gacagctaca gcgactgtgc aagtagctgc tgacataaaa
    61501 agacttttaa aaattccaga aaacaaccat attcagactg gttttgaacg agaaaatcta
    61561 cgatttcaag taattaaaga tcaaaaaaaa gaacaatatc taatcgaata tttaaagatc
    61621 aacaaaaatc aatctggtat catttatgca gcaacgagaa aagaagtaga ccgtctctat
    61681 catttgctta aaaaattcga tttttccgtg ggtagatatc atggtggtct aaatgagaat
    61741 gagcgaacag aaatgcaaga agccttttta tacgatcgct tgcagctgat tgtagcgaca
    61801 aatgcatttg gtatgggtat aaataaaagc aatgtccgtt ttgttatcca ttatcaaatt
    61861 cctggctctc tagaagcata ctatcaggaa gctggtcggg cgggaagaga tggtctttcc
    61921 agtgaagcca tcctgctttt ctctcctcaa gatatccaag tccaaaagtt ttttatccag
    61981 cagtcccaaa gagaagaagg gcaaaagcaa aaagaatatg aaaaattaaa agcgatgaca
    62041 gaatacgtgt atattgagtc ctgtcttcag caatacatcc taaactattt tggcgagaca
    62101 agcagtccgt gcaaccgttg tggtaactgt ttagatgaca gagaaatagt agaagtaacg
    62161 acagaagcac agatggttct ttcttgctta aaacgtatgg gtgagaacta tggtaaacaa
    62221 atgctgatga aagtcttagc aggatcgaaa gaacaaaagc tgcaagcttt aggttttgga
    62281 catttatcta cctatggact gatgaaaaac caatcgcaaa aagaaacgat gcagttaatt
    62341 gaatatttga tatcgaatgg ctatcttctg accatcaatg gtgagtatcc tgttttgaaa
    62401 gtaacagaaa gagggataca agtattgaaa ggacaagaat ctgtttatcg aaaggaaccc
    62461 aagaaagtcc agcagctatc ggatgaagaa accgatacac tatttgaagt gctgcgggaa
    62521 ctgcgaacgg atctagcatc agaagcaggc gttcccccgt atgtcgtatt ttcagattcc
    62581 acattgaaag aaatgagccg aatacgtcca tcaagcagat tggagatgct tcagataaaa
    62641 ggagttggcc aaagcaagct tgataaatat ggcgaagcct ttttatctcg gataaaaaat
    62701 gcggactcta acgttctgga taagtagaaa gaacgaatac gtaaaaatga ataaaaacaa
    62761 ctggttaaat ctgttttttt tgctccttat gaaaatcaaa tatgttataa ttaaggaatt
    62821 agaatgggag gtaagcacag atggattctt tgtcgaaaaa acttgcgatc caagtattaa
    62881 atgatgaagc agaacggatc aaacgattaa tccgaaatca aaacaacagt ctctgtatct
    62941 cacaatgcaa agccttcgaa gaagtggtag atacacagat gtacggcttt tcccggcaag
    63001 tcggttttgc tgtccgtata ggagtaatcg gaaagaacga gggccagctg atgttaagtg
    63061 agctagaacg agaattgaat cagttatata gtgaggttta ccaagaaatt tatgataaaa
    63121 aagaagttgg caaggaggaa tagacttgcc taagaaagtg aaaaaaaggc atctattaga
    63181 ctatagcatc ttgattccct atttaatttt atgtgtattg ggattgatca tggtatatag
    63241 ttctacgtcg tatctattat tagaaaacgg acaaaatccg tccgcatcag tcattaacca
    63301 gagtattttt tgggtgctta gcttgattgc tatcgcacta ctttataaaa tgaaaacaga
    63361 tgttttgaaa aaccagcgtt tgatcatggc agccatcgca gttttgacga ttttgctgct
    63421 gattgttgtg ttttttggta aggagatcaa tggggcgaaa ggttggctgc aaattgcagg
    63481 attctcgatc caacctgccg aatacctaaa aatcatttct atctggtacc tgtctctgac
    63541 tttatctaaa agacaaaaca gtgtacaaaa agattttctt gggactgtga aacgtccact
    63601 tgcaatggtt atcggactta ctgcattagt cgctattctt cccgattttg gtaatgcggc
    63661 agttatcttt ttgattattt tggttctgct tttagccagc ggagtcaatt atgtgtatac
    63721 tttgattgta ggagtaggag gcttttgtct aagtactttt acgatctggc tgatcaatat
    63781 cacaaacgga aagatcttac ccggacgttt acagtatatc tataatcgat ttgctattta
    63841 ccaaaatcct ttctcagacg aattaaataa agggcatcaa ctagtcaacg gctactatgc
    63901 catgttcaac ggaggactat ttggcagggg attaggtaac agtatccaga aaaaaggatt
    63961 tttacaagaa gcacaaaccg attttattta tgcaatcgta gtggaagaac tgggagtgat
    64021 tatggggatt ttgatattag cgttgctatt ctttatgatt gtgcgcattg tccttgtagg
    64081 tatccgttca aaagatccgt tcaattcgct tttgtgcatt gggatcggcg caatgttttt
    64141 gatccaagta tttgtcaatc ttggaggaat tactggcgtt attcctttga cagggatcac
    64201 gttcccattt ttaagtcaag gaggctccag tttgctgatg ctttcgattt gtgtcggctt
    64261 tgtcctcaac atcagtgcgg atgaaaaacg caagagctta ggtttataat aaaaaaagat
    64321 gtaggagtgg ttcgatgaaa aaagttttag ttgctaacag gggtgaaatc gctgtacgtg
    64381 tatttcgtgc ttgtacagaa ttaggaatta aaactgttgg aatctatgca gaagaagatg
    64441 aatattccgt acaccgtttc aaagcggatg aagcatattt agtaggacaa ggaaaaaaac
    64501 cgattgatgc gtatctagat attgaaggga tcatttctat cgcgaaagaa tgcggagctg
    64561 acgctattca tccgggttac ggtcttttgt cggaaaactt gaattttgcg caacggtgcg
    64621 aagaagaagg catcattttt gttggaccca aactgcatca tttagatatt tttggagata
    64681 aaatcaaagc aaaagctgca gctatcgaag cggggattgc ttcgattcca ggcacagatg
    64741 gaccaatcgc ttctatagac gatgccttgg agtttgcgaa acaatacggt tatccgatta
    64801 tgatcaaggc agctctaggc ggcggcggac gcggcatgcg tgtagctcac gatgaaaaga
    64861 gtgcaagaga gggctacgaa cgtgcgaaaa gcgaagcaaa agcagccttt ggtagtgatg
    64921 aagtgtatgt tgaaaaatac atcgctaatc ctaagcatat cgaagtacag attctcgggg
    64981 atacgcatgg aaacgtcatc catctttttg aaagagattg ttctgttcag cgccgtcacc
    65041 aaaaagtagt agaagtagcg ccttgtgtct ctatgaatga acagcaacgt caaaaaatct
    65101 gccaggcagc tgtccaattg atgaaatatg tcgggtatgt caacgcaggt acagtagaat
    65161 ttcttgttga aggagatgat ttttacttta tcgaagtcaa ccctcgcgtc caagtggaac
    65221 atacgatcac agaaatgatt acagatattg atatcgtaac aacgcaactg ttgattgctc
    65281 aaggtttgga tctgcataaa gaaatcggat tgcctcagca agaaggaatt aaattaaatg
    65341 gttcagccat tcaatgccga atcactacag aagacccgtt gaataacttt ttaccagaca
    65401 cagggaaaat cgatacgtat cgttcacctg gtggttttgg cgtacgtctg gatgtcggaa
    65461 atgcttacgc gggctatgta gtgacaccgt attttgattc attgctagta aaagtatgta
    65521 cgcatggtgc tacctttgaa acagccattc agaaaatgga acgttgcttg aaagaattta
    65581 gaatccgtgg agtgaaaaca aacattccat ttatgctcaa tgtcatcaca catcctgaat
    65641 tccaatcagg aaatgcaaaa acaaccttca ttgatagtac agcaacatta tttgaatttc
    65701 ctcgactacg agaccgcgga aacaaaacga tgaaatatat cggtgaaatt actgtgaatg
    65761 gttttccagg tatcgaaagt ggcgaaaaac cattttatga agaaccacgt atgcctaaag
    65821 atctaatcac aagaactgat tacgtaacgg ctaagaatgt attagatgcg aagggtgccg
    65881 atgctttggt cgaatggatc aaaggacagg aaaatttgtt gttgacggat acgacattta
    65941 gagacgccca tcaaagtctg ctagcaactc gtgttcggac aaaggatttc aagcagattg
    66001 cacgtttgac tggagaaggt cttcctgaac tcttttcaag tgagatgtgg gggggagcaa
    66061 catttgatgt ggcttaccgc tttttgaatg aagatccttg gcaacggttg agaaaaattc
    66121 gttctttaat gcctaatacg ttgctgcaaa tgcttttcag aggttcaaat gccgttgggt
    66181 attctaatta tcctgacaat gtattggtgg aatttgtcaa agaagcagca gcgcaaggaa
    66241 tcgatgtatt tcgaatcttt gatagtttga actggactcc acaaatggaa aaaagtatcc
    66301 aagctgtacg ggatactggt aaaatcgcag aggcagctat ttgctatact ggcgatatca
    66361 acgatccaag tcgggcaaaa tacaatgttc agtattataa agacatggca aaggaactag
    66421 aacaactggg tgcgcatatc attgcaatca aagatatggc tgggctgctt aaaccgcagg
    66481 cagcttatcg tttgattagt gaattgaaag aaacgacaga tctgccaatc catctgcata
    66541 ctcatgatac cagcgggaat ggaatcatca cttattctgc tgcttcaaaa gcaggagtag
    66601 acatcgttga cgtggcaatg agtgcaatga gtgggaatac tagtcagcct agcatgagca
    66661 gcttgtatta tgcactggtc aatggtccac ggctgccaga aatcacgatt gaaaatgcac
    66721 agaaattaaa ccattattgg gaagatgtgc gcatgtatta caaaccgttt gaaaacggct
    66781 tgaatgctcc tgaaacagaa gtatatatgc atgaaatgcc aggaggacag tattcgaatc
    66841 tacagcaaca ggcaaaagct gtcggattag gtcatcgctg ggatgaaatc aaacaaatgt
    66901 atcatactgt caacttgatg tttggcgata tcgttaaagt aactccatca tctaaagtag
    66961 ttggagatat ggcactattt atggtccaaa atgatcttac agaagaagat atttatgaaa
    67021 aaggcgaaac actaagtttc cctgaatctg ttgtgacttt cttccaagga gaactagggc
    67081 aacccgttgg cggatttcct aaaaaattgc aaaagattat tcttaaaggc agacctgcat
    67141 tgaatgaacg tccaggtctt ttggctgaat cagtcgactt caacgaagta aaaaaagaat
    67201 tggcagaaaa aattggctat gaaccaaaac aagacgaagt attaagctat ttgatgtatc
    67261 cgcaagtctt tttggactat caaaaagcat ataatcaatt tggcgatgtt acattactgg
    67321 acacaccaac atttttccaa ggtatacgtt tgggtgaaac aatcaatgta caaatcgaac
    67381 gcggaaaaac gcttatcatt cgtttagatg aaatcggaga acccgatatc gaagggaatc
    67441 gcgtactgtt cttcaatttg aatggacaaa gacgtgaaat cactatcaaa gataattcga
    67501 tcatcagtac ggtccaaacc cgattgaaag ctgaaccgac aaaccgagaa caaattggag
    67561 caacgatgtc tggttctgtt ttggatgtat tagtgaaaaa aggcgataac gttaaaaaag
    67621 gtgatacgtt gatgattact gaagcgatga aaatggaaac ggccatcgaa gcacgttttg
    67681 acggagaagt cgcacatgta tatgtgtctt caggagacac tatttcgtct ggtgaccttt
    67741 tgattgaagt gactgagaaa taataagcga aaggtgggga aacatgaaaa gactgggttt
    67801 gttttggctg attctgctat tagttattgt ggcattctac atggaaccag tcttgtttcc
    67861 gccaaaactt gaagtgaagg aaaactctga tcaacaaata tcaaaccaaa tgacaacgtg
    67921 gaaataccag gagcttaaag caactggttt ttctacttat atcggaaaac cgatcgatca
    67981 gctggaaaag gattttggcc aagcctatga tcgattaact agcggatttg gttttgaaac
    68041 aagatattat gaaaataaag ctagtcatct cacatttgaa gcgaacatcc aagacagcag
    68101 agtcacaact gttaaagtac tgcaaacagg gcctgctgat atcgcgccgt tttatttagg
    68161 gatgacgatg caagatctta cgaaaatcac tactatctat actaatttca cttttgagta
    68221 ccatgataca gaagttggaa tcgaactaat ggaagaggat atgaattatc gtccgctaat
    68281 tgcttttgat aacgacactt ttgctatttt attttttgat caaacaacaa acggcctatt
    68341 ttcagttgtg tatttagata aagatagtct tttgaaacta ttgccatatc aagtgtttgg
    68401 tgaaggcctt ccacattatc aagcagataa atcggctgat tgggaatcaa tcaatcgcgt
    68461 taaagaacga aaaagtacga ctttattaaa tatgcttaga agagaagacg aacttcccat
    68521 atacaatcaa acacttgcct ttcaaaacca cagtcagctg ctactacatg attttttgac
    68581 aaggcctgag gaaatattgt cagaggaacg tatggctgat tggcaaaaat cgctaggcag
    68641 tgctcaaacg acagtgaaat tctcactgac gaatgatgaa ttggagaagt taatcagtaa
    68701 acaaaaatta gaaaatacta gtgggctatt tattcatccg gcgattgacg ctactttttc
    68761 gtttttatat tggtacagcg atccttacaa ccatgaccgt ttcatggacc cgggaacgga
    68821 ttctgtaggc attgcttttt ctaaagaaaa tatgttagtc ttattgcaag aagaaaacgg
    68881 agaaagtagt gatagcagtg atcgttaacg aagagctgtt tcaactggag gatcaatgcc
    68941 tccaagtcgc tgaaatgata aaaaagagta aaacgatggc ttcttatctt gaacataaac
    69001 agtcaatgga agaatctgca aaagtttgcc agttgaaaga agcgttcttg tctaagaaag
    69061 aagcatttga aaatgtagcg gcatatggtg agtatgcacc tgattatcgt gaaaaaaaac
    69121 gagctgttcg aaaagcaaaa agacagcttg atatggatga gcatgttgca gctttccgat
    69181 atagtgaaac acagctgcaa agtctgttgg atcaaattgt ccaagtactt gccgaagaag
    69241 tttctaatga aatcaaagta gatgcaggta gcccgttttt tgaaacgaaa aaacactttg
    69301 gatgcggagg taattgtcat gcaattagac aatagtgaat ttgaaataac aaaacgacgc
    69361 gggctgatcg tatgggtcta cagcttaaaa caattaaaga atctgaaaaa gttcggcttg
    69421 ttgcattatg tctcaagaag aatgaaatat gtcgtgcttt acttaaatga agagacattg
    69481 gaagaaacag aagaacgaat ccgtaaatta cattttgttc gaaacgtaga acgttcttac
    69541 cgaccggata tcgaaatgaa ttttgccgaa aaaataggaa acaaaacagt attaaaagac
    69601 gaaggtttcg aaattgaaga gctaaataca gaaatccgat tagctgaaca tctttaaaaa
    69661 tctcttatga gcagttttta aataaaaaat aacttggata acgatcgatt tttgatcatt
    69721 gtccaagtat ttttttggta tactagtacc agtaccattt ctaaggagtg agtagatgcg
    69781 gataatatca ggaaattatg gcgggcgccg tttaaaaagt ttagcagggg cgaacaccag
    69841 accaactaca gataaagtaa aagaatccat attcaatatg attggaccat attttgatgg
    69901 agagacagta ttggatctgt ttgcaggaag cggtggttta gctatcgaag cagtatctag
    69961 aggctgcagt catgcggtat gcgtagacaa aaattaccaa gcgctaaaaa tcatcaaaga
    70021 aaacatcgaa attacgaaag aaccagagaa atttaccaca ataaaacttg atgcggacaa
    70081 agcaatcgaa cggctagcag cggagaaaca acaattcgac tacttgtttc ttgatccgcc
    70141 atatgcaaaa caaaaaataa tcgatcaaat ccataaaatg gaagaactgg gacttttttc
    70201 taaaaccgca gttatcgtat gcgaaacgga taaaacagtc gaattgccag aaactgtggc
    70261 tggattccat caaataagaa aacagattta tggcataacg gccgtaacta tctatagaaa
    70321 agagggaaat caatgaaacg tgcattattc ccaggaagtt ttgatccttt tacaaaaggt
    70381 cacttagata cggtggaacg tgcggctaaa ttattcgatg aggtagtaat tggtgttttt
    70441 atcaatactt caaagaaaag tttgtttccc ccagaagaaa gaatgacatt gatcacaaaa
    70501 gctgtttctc atcttcctaa tgtaaaagtc atgcatcaag aaaatcaatt gacggtagaa
    70561 acagcaaaag agattggtgc agatgcgttg atcagaggca tacgaagtat caaagacttt
    70621 gaatatgaac gagagattgc tcagatgaat catcatctat atgatgaatt ggaaactgtc
    70681 ttcttattag caaaaccaga atatagtcac gtgagttcca gtattttaaa agaagtactg
    70741 cactttggcg gagatgtgag cagctactta ccgccagtga tcaacgaagc tttagctgga
    70801 aagcgtgaat caaatgaaag ctaaacaatg gttgaaaaca gtactgatta tcatatttgg
    70861 aatgttgatc attggatttg tagtagttcc cttgccgtat tatgtagaat caccgggagc
    70921 cacgatcgat ctgaaagaac taattacggt caatgataag gaagataaag gttctgggtc
    70981 tttctctctt acctctgttg gtatcaggca agctacaggt ttcttagctt taaaagctaa
    71041 aatgacccct tttgaagaat tgatcagtga agaagaattg actggtgggg cgacaaatga
    71101 agaatataat caaatgcaac agtattatat ggattcttcc caaaatgcag caatcgaaca
    71161 agcgttaaaa cttgcagaca agccttataa aatggaattc aaaggcgtat atgtcatgag
    71221 tgtagagaaa acatcgaatt tctttggcaa gctttcagtt ggagatacag taacaaaagt
    71281 agacgggaaa agctttcaaa gtacagaagc atttatggat tatgtaaaaa gccaaaaagt
    71341 tggacaaaca atcgaaatca gttatttgca taatggtgaa gaaaaaacag cttctggtga
    71401 tctgatcgag ttaccaacag ataaaaaggc aggaataggt atcacgctta ctgaccatac
    71461 cgagatcgaa tcagatacag atgttcgtat taattctggc tctatcggtg ggccatcagc
    71521 gggattgatg ttcactttag aaatctatga acaagtcact ggtaaaaact tgcgacatgg
    71581 taagcaaatt gcaggaactg gaacgatcaa cagtgaagga gaagttggcc gaatcggagg
    71641 aatcgataaa aaagtggcaa gtgcggataa agctggcgta gagattttct ttgcaccaga
    71701 tgatacgatt tcagacgatg ttaagaaaga atatcctgaa ataaaaacca actacgaaga
    71761 agcaaaagct gccgctaaaa aattgaattc ctcaatgaaa atcgtaccag taaaaacagt
    71821 acaagaagcg cttgactatc tagagaaaat gaaatgaaac taaataatta aagaggggtt
    71881 gtgacacgag ttctgtcaca acccctcttt ttcgtcaaaa atcttcacga taaaaataaa
    71941 aacaaaagaa aaaattgacg taattagatg ttgaagggac gattttgcca gagctcactg
    72001 tttcttcaat tttaaatgaa aggagtaatg aatgattttt actggtcatt ctttgaaaaa
    72061 agaacaatga tagaaaaaat cagcaagaaa atactagctt cacccaaatt gatcatttgg
    72121 atgtctatga tagttcttat cttttgtatc gcaagtggtt ttttattatc aaatttgcta
    72181 ttttatagag aaacagaaga gactgctgca aaagaaatac tttcttcgtt tagttccagt
    72241 ggagaagatc catcagctga aggaaacgat gtagaagagg atgctcgtcc aaaggtcatg
    72301 tacaccgata tcaaaggaag tgtgaaggag cctggtatct attccttttc ttcagaagaa
    72361 cgggtctatg atgtactaaa gcgtgcaggt ggattattag aagaagcgga tagcgatcga
    72421 atcaactttt ctgcgaagat agaagatcag caagtcctat atatccctgc tgtaggtgaa
    72481 gagctgccag aacatcttga tcagttggca tcctcggaag gaaaacagcc tacagcagat
    72541 tccgagcctc ccaaaataaa tatcaataca gccactccat cagaacttca gcagattcct
    72601 gggatcggat cagtcaaagc gcaagaaatc attcgattta gagaagaaaa cggatccttc
    72661 cagaaagtgg aagacctgca ggagatttct ggtatcggtg aaaagacagt tgaaaaacta
    72721 aaaaacttcg ttacaattaa atagaatgat tcaaaatcga ctggcacgag caataaatgg
    72781 acgctaatgg atactaagaa taatccaatt ctaagagctt ggcaggacga ggaggtagta
    72841 tttttggata ggaaatagct tgattgttcg caaagaaaca ttaaaaaaac caatgctgaa
    72901 tgtctatctc caaaataaaa tatctgggat tcatattatg aatacggctg tcagcggcaa
    72961 taacagtcaa gctctacgag aacgctttgc caaagatgta cttagctata cagcagataa
    73021 agtatttatt ctcattggga cgaatgatct agcagagcat aaacaacttt ctaaagaaac
    73081 gtaccaaaaa atctgcagtg gatgatagtt cagctgaaaa aagactatag ttctgatcat
    73141 gctttcttta tcacgccacc agcagtcgat gagacaaaac aaaaatatcg gaataacaag
    73201 ttgatcaaag aatatatctc tgttctcaca gaagtagcaa aacgccatga ttgtgaagta
    73261 tcgattttta tacggaattt cttgaacaaa tggattcgaa gaatttttat aatgggctaa
    73321 tggatgacgg acttcatttc ggcagtgaag ggtaggatct tttgtcagac aggatgattc
    73381 gttatattta aagaatattt tgtttaagca atgagtactt tttaatcaac ttgaaaaaac
    73441 ggaggaatat aaaacatgtt agaaaaatct tcaaaagcat gcgatacgaa taaagaaaaa
    73501 gttactgata agtcacataa tgaacgcatt ccttgggacc agtactttat ggctcaggca
    73561 gtactgttat ctttacgtag tacgtgtaca cgtttagaag taggagcgac actcgttcga
    73621 gaaaaaagga tcattgctgg cggctataat ggagcagtca gtggagatgt ccactgcata
    73681 gatgaagggt gttacgtcgt ggatggacac tgcttgcgaa cgatccatgc agagatgaat
    73741 gcactcctac agtgtgcaaa acttggtatc ccgacggaag gttcagaaat ctatgtcaca
    73801 cattttccat gtttggcctg tacaaaggct ttgttgcaag ctggtgtgaa agggatcaat
    73861 tatctccatg actatcgaaa tgatccttat gcccaagcgt tgatcgaaca attaggtgta
    73921 tctgtacacc aagttgcttt agactcacag catttttcaa aacttcaaaa tgaattaaca
    73981 ggaaaacatg tctagcgttt tagaaaggca tgttcgaaat cggttgattt ttccggcaat
    74041 tacgtcgatg attatctgtt tgttgtatta cactgacaat tggggttatc gtttgcttct
    74101 tgccggaatt tttttatggt tttcgctaaa agaaggacgt tttatcgcgt acttgtgtat
    74161 tttgtgtagc ataacggtat ccatttctta taggctacaa gagtattcat ttttagagga
    74221 aaaaacaaca tctgcgcaaa ttactgtatt tacagatacg attcgtgcaa atggtaattt
    74281 tttgacaatg gaaggaaggt tatcagagaa taaacaaaag ctccagcttt cttatcaagt
    74341 aaacacagaa gcagaattga aaacatggct taacttccga ggaaagggaa tagaattgac
    74401 tgctgaaggc gagttcatct ctccagcaaa aaagcggaat ctttataatt ttgaccaaga
    74461 agcttacttc aggaaccata gaatttctgg acggttccaa attcaaaaga ttgaacagat
    74521 caccatgcaa aatcactggc gaaactggct tcagagaaaa agaggatctt ttatctcctt
    74581 tgtccaatct gaatttccgc aaaaaacaag tgtatatatt aattctttat tatggggcta
    74641 taaagatgca ggattcagag aaccagaaga aatttttagg gagagtggtt tgctgcatct
    74701 gttcagtcta tcgggtctac acatccaatt ttatttaggg tggctgtatt atttatttcg
    74761 acgtatcgga tggccactac acgtcagtat tattccgttg agtctactaa ctatctgtta
    74821 tgtttctatt gcaggaagtt ctatttctgt tttgcgtgct agtttgctgt ttttattgcg
    74881 attgctagtc aaattgctga ataagcatta ttccaccatg gacttatttg cagttgtctt
    74941 ttggcttctt ttactatttg atcctcgatt tctttttcat gcgggtggac agcttagttt
    75001 tttcatgagt tttttgttta tcctgatttc ttttgatgga gattggttga aaaaaacggc
    75061 ctctcacgtc ctgtttacta tgatcacgat gccgttgatt gtatggcatt tttatgagtg
    75121 gcctatttta ggtagtcttt ttacattact gattgcaccg gtattcaagt ttctttttct
    75181 tcctggtgtg ttatttctca cactcttcaa tcgctggctg ccggaaactt tacttttgct
    75241 gatagaagag ggattaatct tatttgagcg aataatcgat ttttcttcag ggtcgtctct
    75301 cattatagga cgtttgcccc ttgtgctatc tgggatgtat attttcggat tactgatttg
    75361 tatggatagg ttgaaagaac aacctgtgaa acacttgttt tatgttggtc ttttttcttt
    75421 tatttttttg actttgccat ttttacgctt ttcttcaacg atcgctttta tcgatgtcgg
    75481 tcaaggagac agtatctttt tgcaaagtcc ttttcgaaaa gaaacgattt tgatcgatac
    75541 tggaggtcag ttacactttg aaagagaaaa gtgggcgcaa ggtatgatac gtcctcctgc
    75601 tgaaagaaat atcgttccat atctaaaagg tcaggggata agtgtaatcg ataaactcat
    75661 cttgacacac gacgatacgg atcatgtggg agaattaccc acgttatctc agcattttac
    75721 tatcaaaaaa atttatatcg gttggggcgc tggacgaaat gagccattac aaaggcattt
    75781 atctgaagct caaaaaaatg gtactgagat cattgaaatc aaacatgggg acaggatcag
    75841 cggttattat gatctttatg tattgactcc ttttgaaaag ggagagggaa gaaatgaaga
    75901 ttcgattggt ctatggatgg aatataacaa taggcgcttt ttatttttag gagatttgaa
    75961 tcaagcaatg gaaaaacaat tgcttttgat ttatcctaat ctgaaagccg atgtagtcaa
    76021 attaggtcat cacggtagcc gtacgtcaag taacacagat tttctttccc atattgaagc
    76081 aaaacatggg attatatcct gcggcgtaaa taatcgttat ggtcacccgc acagcgaagt
    76141 attggagaca ctagaagaaa atcagataaa aacatggcga acggatcaac aaggaatgat
    76201 cacctatcgc tggcatccag tttttcatcc tcatgggcta gtggaaacaa tgatagacta
    76261 gtaattctca tctgctcatg gtaggataat ttttgaaagt ggtgagtaat ttgagtatac
    76321 aagcagagtt acaaaaaata cgtaaagaaa aacttgcatc ttgttacttg attttaggaa
    76381 cagaaaagtt tctccaagat caagttcgga cagaaattgt caaaaaaatc caattagagg
    76441 gcgaagatga cttaaatttt ttaagttttg acatggaaaa tgcaagttta gatgaggtag
    76501 tagctgaagc agagacgttg ccgtttttcg gagatcaacg gttagtattc gtagagaatc
    76561 cttatttttt aacaggtgaa aaagtaaaca atggaatcga acaaaatact gatttattga
    76621 cggcttattt gaaagaacca ttagaatcga ctgtattagt attctttgcg ccatatgaaa
    76681 agttggatga acggaaaaaa gtaacgaaac aattgaaaaa aacagcaatt atcatcaatg
    76741 tccaacagtt gaatgaaaaa gaagtacgtc aatatctgat gaatacctta gagaattcct
    76801 caatcaaaat ggaacgaagt gcaattgatt tattcttgag gttgaccgac ttagatttat
    76861 ccaaaatgat gagagagtta cagaaattgg tcctttatgg acaaaatcaa cagcaaatca
    76921 cggtaagaga agtagaagaa ttagttccta aaacgttaga acataatatt tttgacatga
    76981 cccaatatat tttaaaaggg aagacggaac aagctttgcg tttatacgaa gatttagtta
    77041 tgcaagggga agaaacaatc aaaattaatg cgattttgct ttctcaatta cgtttatttt
    77101 tacagacaaa atttcttgta aagattggtt atcagcaggc aaatattgct gaaacattga
    77161 aaatccatcc atatcgtgta aaacttgcca tgcaggaagt aaaaaaattc gatgaacatt
    77221 tgctggtaca gttgtttgat caattagttg agatggatta tcaaatcaaa acgggacaaa
    77281 tcgaaaaaga attaagtttt caactattcg ttttgcgcac agggcagttg ttaagaaatc
    77341 gataagaaga aagacgaggg caaagcatgc gggaaggtcg tttgtttttg tccttttttt
    77401 gacaaataaa aaaatcggcc gaagccgatt cttttattta gcgatttttt tgcttaaacg
    77461 agatttgtcg cggttagctt tgtttttgtg gattagacct tttgattcag ccatatcaat
    77521 agctttaaca gcttctttat ataacgcatc tacgttgtca gcacctgaag ctacagcatc
    77581 ttcaaatttt ttgattgcag tacgcatagc gcttgtttga gatgaatttt tcacattagc
    77641 gttttcgctt gtacgaacac gtttgattgc agattcaatg tttggcatta gtttcacctc
    77701 cgataactta agttcttacg cgtgttaccg cataattcaa cattcattat tatacctaaa
    77761 ggcagcgtgc tttgcaatat gaacatgaca agtttttttc cttaacagtc ttttattagg
    77821 attttttttc agtaaaaacg gatagtacaa gctgaaaaaa atcaaaaaga cgctattgtg
    77881 ggaggcaata gcgtcaaacc aaactagatt tttatttatc tatttaaaca aagtttgaat
    77941 gggaatgttt ggtactaagt tattatataa caaagagaac cgtttgcaaa gttttttttg
    78001 aaaagggata cgattccctg ttgttctttg tttttctatt aagaaactgc agttatttta
    78061 taaccagtat cctttgatca gtaaaggcta ttctggtgta ccgattattt taatgtattc
    78121 atcgatattt tttatcaaca ttttttttat gaccaactgg ttccgacggt tccatttgcc
    78181 acttacttcc agcacatatt tatttagggg caaacgtaag atatattttg ccaaatagcg
    78241 atctttaagc aggccattga tcacacggtc ctcaagctgc aagttgatcc cagtcagcat
    78301 cattggctgt tcttgtaaaa cgtacgtatc aattaaaaga ccttcaaaag tttccacaag
    78361 cgtcttcctt tcttttcaaa gtaacctgaa aacaggcact aattcttcca ttttgatatt
    78421 cgttgaagtg gatgcgatca ttctttcttt tgtccactgt aatctagtat aacaaaaaag
    78481 agcagaagag ccaaaaagaa aaaagagcat cgaattctat aggaagtctc attggcacgg
    78541 cctaaattat tacaaaacat tgattaaaaa aagcccctgc atcgcaggag catcccctat
    78601 atcgttttaa taggtcaatc aaaaagcagt atcaaacaca ttttgtatca aatcaggaaa
    78661 taatagataa gctaagccca gaatcaaaag taccgctaga agaaaccagc cgattgttcc
    78721 tatgaaatac tttaattttt cgaacattgt atcacctcgt tgtcagtata tcacggatca
    78781 gtaaattggg ggtgttaaga atctcctaaa atagacaaaa tttgttctat tttcctaaag
    78841 caaatacttg taagatacag taagtgatac cagaagcaat gaccggtccg gcggcgattc
    78901 ctcttaagaa gacaacacct aaaatcgtgc cgaaaacaag tgcaacagtg atttgtggat
    78961 cagaagcaat caatcctaca cccttggaag ataagacagc cactagccca ccgcaaaaaa
    79021 cagctaccca gccaataggg gatttaaagg cttctaatag atcacgtaga ccgatttctc
    79081 ctgttgcgat agggattaga atagccgctg tgatgatcgt caccccaata ttgattcctt
    79141 tgccttgaag ccaagggaaa aaggaacggg aggctggtat gagcttcata atcaaaacaa
    79201 aagctgccgc tatcatcaga ctttgatttt tagctaaaat tgctacaagt aaaataattc
    79261 ctaaaaaaag ccaactttcc atgaaatata ttcctcactt tcttcgaaac aatcataaca
    79321 tgtttttaga aagttacaaa agtaggaatt catccttcaa gacctattca taagctgttt
    79381 ttttcataaa ataaacaaaa tcataaaaaa acactttgct tatttgacag aagacagccg
    79441 cgctttgtat agtggacaag acttacattt ttttgaagaa tcagaaagat caggtaaagg
    79501 tcttagtaaa cgcaagccat tcagaataac caatattgtg cttccttcgt gtcctacaac
    79561 tccaagaggt agatttaaaa attgcaggaa gttagaagca atcaacaaga gaataacaag
    79621 aatcgaaaaa ataatatttt gtttgatgat ccgccgcatt tttaaagata gccgatgact
    79681 gtagacaaat ttatttaagt cgcttttcat taagacgatg tctgcgatat ccatagcggc
    79741 atctgtccct tgccccatag cgacacctaa agaagcggct gctaaagcag gcgcatcatt
    79801 gactccatca ccaaccatcg catttacttg gtaaagttca ttctcttttt tgactagttg
    79861 tgttttttct tccggagtac agcttgcata gtaatgttca atccctactt gttcagcaat
    79921 ggcctttgct gtgccagaat ggtcgccggt tatcatactt gtatggatgt tttgggattt
    79981 aaagtaggaa attacttgtt gggtatttgc ttttggaata tctagcagac ctaaaacagc
    80041 aattaactgc tgatctttag aaagataaat cacggttttg ccttgatttt ccaaacgttc
    80101 gatcatttcc gcaatctctt tgctgattat catcgtctca ggatcatatg cgtgcttgcc
    80161 aactttccat tgcgcaccta aataggtagt ttgtaatcca aaaccagtaa tttcttctat
    80221 tggtaagtat tggatttctt caggtattgt gcaatcgaaa tattgcaaaa tcgcatgagc
    80281 aagtggatgc gtcgtttttt gttccatagc aacaagaagt tgttgtgcca aatccttttc
    80341 atcaaagaaa aagctgtcag ttacaactgg ttttccttta gtgagcgttc ctgttttatc
    80401 aaaggcaatc gcttttaatt cagaaagctt ttctaaatgg ataccgcctt tgattaaaat
    80461 cccatttttt gcagaatttg aaagagcagc tagagaagct ggtgtagcag atgcgacaag
    80521 cgcacaagga gaagccacaa ctaataatac cattcctcga taaaagcttt catcccaact
    80581 ccaacctagg aaaaagtggg ggagaaggat cattagagga acagcaagca aaatcaattt
    80641 tacataatta ttttcgattc gttcgataaa tgaagctgtt tgagttggcg tattctgcgc
    80701 ttcttctact aattggataa tcttagcaaa gacagtttcg gaactcgttt ttgttactcg
    80761 aatcgtgatg ggatttcctt gattcagcgt tcctgcatac atctcatcgc ctaaatattt
    80821 ttctacaggc acagattcac cgcttaatgc agactcatcg atcgttgcct gtgtgctttc
    80881 taaaaagcca tctatcggaa cagctgctcc tttagcgaca aataccaagt tatccacctt
    80941 tagttgagaa acatctactt cttgcatttg tccattttct tttaataatt gggctttttg
    81001 tggttgaaga ttcatcaaag cagtaatttc tttttgactt ttattagttg tgtattcctc
    81061 taatgcaccg cttaaacaaa agatgaatgt caacattgct ccttcaaagt aattaccgat
    81121 caaacaggca ccaatagcag ctaaagccat caataaatcc acattcagtg tccgatcatt
    81181 ccacaattct ttcaagcctt ccgatgtttg tttatatcca ccgatgataa tggcactgat
    81241 ataaaaaagt ggggtgatgg taagagagaa tcggtcgaag ataaaaccga agatcataaa
    81301 taaaaggcag ccgattgttg ctgcaaaagc ttttttctga ttagtcatcg ttattagacc
    81361 tccttgtcaa aacaaggata gcataataaa aatgtaattg ataataataa cagctaaatg
    81421 agaacgataa tggttatcat ttggtgatta agcttgatgt tgtttttatt atttataatg
    81481 attattatta aagaaaataa aaaaccagaa catttctcaa ataagagaag tgttctggtt
    81541 ttttctacat tgcaataaac caataaccaa tcaaaatgat accaaggata atacgatacc
    81601 agccaaaagc ggtaaagtcg tttttcttca aatagcttag taagaatttg atagctaaga
    81661 cggaaaccac aaaagatacg aatgttccta caagaagaac aacagtttct tccattttga
    81721 atgaattccc ttcagctaaa tatttgacga tcttcaagcc gcttgcgcca aacatgacag
    81781 gaatccctaa gaaaaatgaa aactctgtag cgacaaaacg cgaagcacca atcaagatag
    81841 atcctaggat cgttgcgcca gaacgggatg ttccgggaac aagtgccaag acttggaata
    81901 acccaacaag aagagctgct tggtacgtaa agtcattcag agaagtccaa cgaggtgttt
    81961 tgtttttgtt gcgtttttcg acaataataa aggcaatacc gtaaacgatc aaaacaatgg
    82021 caactggtaa gaacttgtgg aaatttgctt ctaaccaatc atccaaaggc aaaccaatga
    82081 tgatggctgg tatgcaagca acgatcactt taaaccaaag tgtccacgta tctttttttt
    82141 ctaccgttga tttacttgga gaaaatgggt ttaacttgtg aaaataaaga acgacgacag
    82201 ccaagatcgc ccctaattgg ataacgacat tgaacatcga cataaattgt gaactggcat
    82261 ccagtttgat gaattcatct gctaggatca aatgaccagt actactgatg ggcagccatt
    82321 ccgttattcc ttcaataata ccgagaatca ctgctttgat aatatttgca aaaaacataa
    82381 attccttcct tcccaattaa gatacatctt atagtatacc gttatttttt gagaaaccca
    82441 atctgttttt taatattaag aaaaacttga gaaataaagc agataagtac acgaagagaa
    82501 aaaagaagac aaagccctcg ttcagacttt gccttttatg attctctggt aaaaattaaa
    82561 atatccccat tatagaaacg agtcgcgact atgtgttttc ccgttatcga tataagacgt
    82621 atcattgatg ccgtggatag aaaatcgacc gtttgcataa gtgacctttg ttacgctgcc
    82681 atttaaaagc ccagcacgaa caggctgttt aggatcgatc agattaagca aaaaggcgat
    82741 cgtcaaacca tgagatacga ctaaaacatt tccaccacca tttttttcgc agtgatgggc
    82801 gatatcttca aatcctgtcc atacacggtc tcgcaatgtt tcataagttt ccgcccaccc
    82861 agcggtatct aagttgtata cagcattagc aatttgttca aaagaaactt ctgtggtaaa
    82921 catttcatca tatgttttaa aattcaatac acgagggatc acaccccaca tttccatgtc
    82981 atatccacct tcaagagacc cgaaacacca ttctctaatc cttttttcaa taaaataagg
    83041 aatttctttt cccttagaat gttctcctag aataatacgg gcagtttcga ttgcacgacc
    83101 actgtcacta gaataagcaa atgaaaagtc gacatttttt aacccgcgac ctaaataatg
    83161 aatgccttct tgtccttgct ttgtgagggg tgtatcacac cagccttgta ctcgttcaat
    83221 tgtattgaac atcgtttttc cgtgtcgtac gatataaagt gtagtttctg tcattttatt
    83281 ctcctcctaa gtcgttttaa cgattgaaaa aaggatttgt ccttttttcc tgttcaatcg
    83341 tcgttgcatc gccatgtcct ggatagactg caaattcatc aggtaataca aacagctggg
    83401 tccgaatact atgaagtaat tgttctaaat tccctgtata cagatcggtt ctaccgatac
    83461 ttcctctaaa cagggcgtcg ccagaaacga caaaatcatc aaaaataaag ctgacgcttc
    83521 ctatagaatg ccctggggta gggacgacgc gaaaagacat gtttcctaaa gtataagttt
    83581 tcatttcaaa ttcatattct gcaggcgaga caataacatc agcaatatca tcatgtctac
    83641 cgagtccaga aagattcaaa atcggatcac ctagccattt ttgctctagt gggctgacat
    83701 aaactgggat ttgataatac gcgcgtaaag gttccactgc accgatatga tcgtagtgag
    83761 tatgggtaag caaaatcgct accggctgtt ggtttgtttt tttgatttct tgtttgattc
    83821 gttccgcatc ttctccaggg tcaatgatca aaagtgcttc ttcgttgtag accaaataac
    83881 agttttcttc aatcattcct gtttttaatc tttcgatatg catgtgcatt cctccttgat
    83941 agccaaaact tctttcatcc attagtataa cgcattcatt gctaggaaac aaaaacaggt
    84001 acttatttct ctttacttat ctgaattatc agactatatg aaaagtattt ggatattcta
    84061 aagagaagga aagtaagttt tcatagcatt ttaatatgtc cttgttacat tagattcatc
    84121 caaaaatcca aattctatat cctatatccc tgcctaatca gcagggtgtt tttttatgtt
    84181 tggaaagaga gctctttttg ctaaactagg ttttttcagg taatcttaga gagaaagagg
    84241 tggtggaaat gatcaaacag tcaaaaatat ctgtgcggca cttagtagaa tttattttgc
    84301 gacgtggaag tatcgacaat cgtaaaaaaa gtaaccatac tgcattagaa ggagcgagaa
    84361 tccaccgcaa gctccaaaaa gatgcgggag aaaactatga aaaagaagtc tttttaaaaa
    84421 caacagttca attagatgaa tattacctga tcgttgaagg ccgtgcagac ggtattttca
    84481 aaagagacgg tatttattat atagatgaaa tcaaaacctc cgaaccacga tttgaagatt
    84541 tagaaccaga acaagtggaa cttttctttc atcaagcaag ggtgtatgcc tatatctata
    84601 gtcaagaaca tgatttacaa gaaataaatc tgcaattgac ttacttccag acggcagaag
    84661 aagtgatcac gagaaaggtt gaacatcaga caagagagca gttagaaact ttttttaaaa
    84721 agctgactga agattataaa gaatggcttg tttttcaaga aaactggcgc actgtacgca
    84781 atgcctcttt gatggctttg aaattccccc atgatgagta taggaaaggt cagcgtgaac
    84841 tagcggtagc tgcttataaa acgatccgta cgaaacagaa attgtttgta gaagcaccta
    84901 ccggaacagg aaaaacaatc tctacgttat tccccgcttt gaaagctgta ggggaagaag
    84961 agggagaaaa gatcttttat ctaacggcta aaacaataac tagacaagta gctgaagatg
    85021 ctatgacagc attaaaagat acaggagctg aagtaaaaag tgtcactttg acggcgaaag
    85081 acaagatctg ctttttagat gagacgacct gtaatccaga tcagtgccca tatgccaatg
    85141 gttattacaa tcggattaat gaaggtttat gggatctttt gaatcatgag aatcagatca
    85201 ctcgtgaagt catcgaaact tatgcaagaa agcatacact ttgtcctttc gaactatcat
    85261 tagatgtcag tatctggtgt gacgtaatca ttggggatta caattattta tttgatccaa
    85321 ctgtttattt aagacgtttt tttgaagatg aaaaaaacga ggactatctg tttttgattg
    85381 acgaagcaca taatttggtg aatcgttccc gagagatgta ttcggcagaa ctatcttatg
    85441 aaaaaacgaa acgaagtaag gaagctatac caaaggaatt caaaaaactt catcgccgtt
    85501 tcaacaaact attaaaagaa tttgattcga tccgagaaat agcaaaagaa gatcattggg
    85561 attatcatca tcaaaaagca ccggctgaat ctttagtaaa ggcaggttat caattaagcg
    85621 aaaaaattaa agaatggctg gcagaatttc ctgagcaccc atcacaagaa caattgcttc
    85681 cttattattt tgatttgctt cattttttga aggtcagtga attttatgat gatcactacg
    85741 aaacaacagt cgaaaaaacg tatcgcgatc tgatagtaaa agagttttgt attgatccaa
    85801 gcttgtttct tgagcagtcg ttggataaag gaaaaagcag tattcttttt tctgctagtt
    85861 tttcaccact ctcttattat caggaaacac tggggggaca aaaaagtctg gcttacaaac
    85921 tgccgagtcc tttcccagaa ggaaaccagc aagttttcat cgcaaattac ctagagacga
    85981 cttatcgaaa aagggaacac agtctggcaa aattagtaga gacgattcat gcttttgctg
    86041 atggaaaggt cgggaattat ttcgttttct ttccttctta tcaatattta gatcttgcgg
    86101 tggaagcatt cagacagcaa tatcccgaca gcaatgtgct aatccaagaa acggacatga
    86161 atgaggaaga acgcgaacga tttttaacaa aatttcaaac aaatcccgct gaaacattga
    86221 ttggattttg cgtacttggc gggatttttt cagaaggaat cgatttgaga ggggaccgat
    86281 tgatcggcag tatgatcgta ggtgtaggtt tgccgcagat gaatcatgag caggaactga
    86341 tcaaatcata ttatgatgag aaggaacacc ttggatttgc ttacgcttat cagcttcctg
    86401 gaatgaacaa agtcttgcaa gctggtggtc gagtgattcg tggcatggcg gatcaaggaa
    86461 tcgttgtatt ggcagatcaa cgattttcta gtttttctta tcgacgttta ttcccacaac
    86521 attggcaaac agcaagagaa gtacgaagtg ttggggaatt ggaagaagga atacagcgtt
    86581 tttggttatc aaaagaagca gtaaatcaaa aaaacgagga atgacagacg tcagcctcgt
    86641 tttttgatta acgatagtta gtgaattgca actcgattgg taagtcaagt ttacgtaata
    86701 aagcaatcac agcttgtaga tcatcacggt tcttacctgt cactctaatc tgatcttctt
    86761 ggatttgtgc ttttactttc agtttttcat ttttgatagc tgtagtgatt ttcttcgcgt
    86821 tttcacgatc gattccgttg accaaatcgg cagtttgtcg agctttgcca cctagagcgt
    86881 gttctgattc ggaaaaatgg atatttttga ttggcacatt tcttttgatc agcttaccaa
    86941 ataatacatc tttgatctgt tcgattttga aatcatcatc gctcaataca actagattgc
    87001 tgttctctaa tttgatttct actgtagaat ccttgaagtc aaagcgattg gacaattctt
    87061 ttgttgtttg ctgaatcgca ttttttactt cttctgtatt gacttctgat acaatgtcga
    87121 agctagcatc ttttgccata ggaaccctcc tgatatacat ataaatattt accatacatc
    87181 tatcttatca taggattagg gcagattctt acctgtttgc taaaattctt ctcatttgag
    87241 ttcgaagtat tttattttaa tagataaact gttattctta catttggaat accaaagtag
    87301 gggagaggtg cttgtgagta aaattatgac tgcgtatcat aaaaatgaaa gattaaagaa
    87361 aatagtggtt attttgataa ccggattgac cgctgcggtc gctttaaatt attttttgat
    87421 tccagccaat gtcttttctg cagggatgaa tgggatcgct caaatcattt cctcgctttt
    87481 atatgaatgg tttcatatca agatagacac aggaacattt atttttcttt tgaatatccc
    87541 tgtgtttgta ttagggtttc tcaaagtagg aaaacgagca acgatcctta gttttgtgaa
    87601 tgttgtatgt gtatcggtga tgacgatgct gcttccagtc ggacagatca ctgataatat
    87661 tttgatgaat gccctagtag gcggtgtttt attaggtctt ggcgtaggat tatctttaaa
    87721 aatgggcttt acaactggtg ggatggatat catctctctt gtactttcac aaacaactgg
    87781 aaaaacagtc ggaaactaca tgctgattct aaatggtatc attgtagtca tcgcaggctt
    87841 cttctttaac tgggaaagtg ccttgtatac gattatttcc atttatgcaa tgactcaagt
    87901 aattgatgcc atccacacga gtcatcaaaa agtaacagca atgatcgtta ccgtcaagcc
    87961 agaagaggta acagaagaac tagcaaaacg gatggtacgt ggaatgacat tactgccgtc
    88021 tgtcggtggc tattcaaaaa aacaagggaa catgatcatg atggtcatca cccgctacga
    88081 aatgtatgat cttgatcaga tcgttcatga aatcgacgac gatgcattta tcaatatttt
    88141 accaactcat tcagtatttg gccggtttgc aaatgaaaac gaacaaaaaa tgtatcgaac
    88201 aactggcgta ttgccagaaa taaaaaatcg ttcacaaaaa aaatgaggaa tcaggtttat
    88261 tttagaataa atttaatcta ttacatctaa taagaacatt tttataaatt taaacga
//
LOCUS       DACDIE010000005        80456 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.005, whole genome shotgun sequence.
ACCESSION   DACDIE010000005 DACDIE010000000
VERSION     DACDIE010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 80456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 80456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..80456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.005"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(278..676)
                     /locus_tag="IV250_000424"
     CDS             complement(278..676)
                     /locus_tag="IV250_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAP8767737.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(673..1446)
                     /locus_tag="IV250_000425"
     CDS             complement(673..1446)
                     /locus_tag="IV250_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP8767738.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1443..2126)
                     /locus_tag="IV250_000426"
     CDS             complement(1443..2126)
                     /locus_tag="IV250_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP8767739.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2293..3180)
                     /locus_tag="IV250_000427"
     CDS             complement(2293..3180)
                     /locus_tag="IV250_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8767740.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3177..4046)
                     /locus_tag="IV250_000428"
     CDS             complement(3177..4046)
                     /locus_tag="IV250_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8767741.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4183..5469)
                     /locus_tag="IV250_000429"
     CDS             complement(4183..5469)
                     /locus_tag="IV250_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8767742.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5742..6320
                     /locus_tag="IV250_000430"
     CDS             5742..6320
                     /locus_tag="IV250_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP8767743.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6320..7249
                     /locus_tag="IV250_000431"
     CDS             6320..7249
                     /locus_tag="IV250_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8767744.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7246..8982
                     /locus_tag="IV250_000432"
     CDS             7246..8982
                     /locus_tag="IV250_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP8767745.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(9045..9881)
                     /gene="pheA"
                     /locus_tag="IV250_000433"
     CDS             complement(9045..9881)
                     /gene="pheA"
                     /locus_tag="IV250_000433"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAP8767746.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9878..10387)
                     /locus_tag="IV250_000434"
     CDS             complement(9878..10387)
                     /locus_tag="IV250_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAP8767747.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10380..11675)
                     /gene="aroA"
                     /locus_tag="IV250_000435"
     CDS             complement(10380..11675)
                     /gene="aroA"
                     /locus_tag="IV250_000435"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAP8767748.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11688..12779)
                     /locus_tag="IV250_000436"
     CDS             complement(11688..12779)
                     /locus_tag="IV250_000436"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAP8767749.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12796..13962)
                     /gene="aroC"
                     /locus_tag="IV250_000437"
     CDS             complement(12796..13962)
                     /gene="aroC"
                     /locus_tag="IV250_000437"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAP8767750.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13964..15028)
                     /gene="aroB"
                     /locus_tag="IV250_000438"
     CDS             complement(13964..15028)
                     /gene="aroB"
                     /locus_tag="IV250_000438"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAP8767751.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(15061..16083)
                     /gene="aroF"
                     /locus_tag="IV250_000439"
     CDS             complement(15061..16083)
                     /gene="aroF"
                     /locus_tag="IV250_000439"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAP8767752.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(16742..17716)
                     /locus_tag="IV250_000440"
     CDS             complement(16742..17716)
                     /locus_tag="IV250_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HAP8767753.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(17697..19580)
                     /locus_tag="IV250_000441"
     CDS             complement(17697..19580)
                     /locus_tag="IV250_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HAP8767754.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDKITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(19598..21823)
                     /locus_tag="IV250_000442"
     CDS             complement(19598..21823)
                     /locus_tag="IV250_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP8767755.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEEKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            21938..22972
                     /locus_tag="IV250_000443"
     CDS             21938..22972
                     /locus_tag="IV250_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8767756.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(23039..24052)
                     /locus_tag="IV250_000444"
     CDS             complement(23039..24052)
                     /locus_tag="IV250_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8767757.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(24130..24318)
                     /locus_tag="IV250_000445"
     CDS             complement(24130..24318)
                     /locus_tag="IV250_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAP8767758.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(24758..24886)
                     /locus_tag="IV250_000446"
     CDS             complement(24758..24886)
                     /locus_tag="IV250_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP8767759.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(24889..26262)
                     /gene="rlmD"
                     /locus_tag="IV250_000447"
     CDS             complement(24889..26262)
                     /gene="rlmD"
                     /locus_tag="IV250_000447"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP8767760.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(26373..27428)
                     /locus_tag="IV250_000448"
     CDS             complement(26373..27428)
                     /locus_tag="IV250_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP8767761.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(27438..28868)
                     /gene="gatB"
                     /locus_tag="IV250_000449"
     CDS             complement(27438..28868)
                     /gene="gatB"
                     /locus_tag="IV250_000449"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAP8767762.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(28865..30337)
                     /gene="gatA"
                     /locus_tag="IV250_000450"
     CDS             complement(28865..30337)
                     /gene="gatA"
                     /locus_tag="IV250_000450"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAP8767763.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(30337..30642)
                     /gene="gatC"
                     /locus_tag="IV250_000451"
     CDS             complement(30337..30642)
                     /gene="gatC"
                     /locus_tag="IV250_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAP8767764.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(30748..32784)
                     /gene="ligA"
                     /locus_tag="IV250_000452"
     CDS             complement(30748..32784)
                     /gene="ligA"
                     /locus_tag="IV250_000452"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAP8767765.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(32801..35035)
                     /gene="pcrA"
                     /locus_tag="IV250_000453"
     CDS             complement(32801..35035)
                     /gene="pcrA"
                     /locus_tag="IV250_000453"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HAP8767766.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHHI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            35190..36749
                     /locus_tag="IV250_000454"
     CDS             35190..36749
                     /locus_tag="IV250_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HAP8767767.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVVGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(36860..38791)
                     /locus_tag="IV250_000455"
     CDS             complement(36860..38791)
                     /locus_tag="IV250_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP8767768.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(38809..39723)
                     /gene="pfkB"
                     /locus_tag="IV250_000456"
     CDS             complement(38809..39723)
                     /gene="pfkB"
                     /locus_tag="IV250_000456"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAP8767769.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(39720..40472)
                     /locus_tag="IV250_000457"
     CDS             complement(39720..40472)
                     /locus_tag="IV250_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP8767770.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(40631..40840)
                     /locus_tag="IV250_000458"
     CDS             complement(40631..40840)
                     /locus_tag="IV250_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8767771.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(40851..42179)
                     /locus_tag="IV250_000459"
     CDS             complement(40851..42179)
                     /locus_tag="IV250_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8767772.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            complement(42544..43131)
                     /locus_tag="IV250_000460"
     CDS             complement(42544..43131)
                     /locus_tag="IV250_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAP8767773.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(43214..44464)
                     /gene="clpX"
                     /locus_tag="IV250_000461"
     CDS             complement(43214..44464)
                     /gene="clpX"
                     /locus_tag="IV250_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAP8767774.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(44711..46000)
                     /locus_tag="IV250_000462"
     CDS             complement(44711..46000)
                     /locus_tag="IV250_000462"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAP8767775.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(46154..47086)
                     /locus_tag="IV250_000463"
     CDS             complement(46154..47086)
                     /locus_tag="IV250_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8767776.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHQVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(47243..47902)
                     /locus_tag="IV250_000464"
     CDS             complement(47243..47902)
                     /locus_tag="IV250_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAP8767777.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(47905..48930)
                     /gene="sppA"
                     /locus_tag="IV250_000465"
     CDS             complement(47905..48930)
                     /gene="sppA"
                     /locus_tag="IV250_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAP8767778.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(48943..49233)
                     /locus_tag="IV250_000466"
     CDS             complement(48943..49233)
                     /locus_tag="IV250_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8767779.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            49642..51447
                     /gene="glmS"
                     /locus_tag="IV250_000467"
     CDS             49642..51447
                     /gene="glmS"
                     /locus_tag="IV250_000467"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAP8767780.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(51519..52874)
                     /locus_tag="IV250_000468"
     CDS             complement(51519..52874)
                     /locus_tag="IV250_000468"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAP8767781.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPSDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(52898..54067)
                     /locus_tag="IV250_000469"
     CDS             complement(52898..54067)
                     /locus_tag="IV250_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8767782.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(54064..54951)
                     /locus_tag="IV250_000470"
     CDS             complement(54064..54951)
                     /locus_tag="IV250_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAP8767783.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTRAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(55140..58832)
                     /gene="nifJ"
                     /locus_tag="IV250_000471"
     CDS             complement(55140..58832)
                     /gene="nifJ"
                     /locus_tag="IV250_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAP8767784.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMTIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            59031..59552
                     /locus_tag="IV250_000472"
     CDS             59031..59552
                     /locus_tag="IV250_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP8767785.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            complement(60251..60816)
                     /locus_tag="IV250_000473"
                     /pseudo
     CDS             complement(60251..60816)
                     /locus_tag="IV250_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(60995..61651)
                     /locus_tag="IV250_000474"
     CDS             complement(60995..61651)
                     /locus_tag="IV250_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8767786.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            complement(62219..63154)
                     /gene="whiA"
                     /locus_tag="IV250_000475"
     CDS             complement(62219..63154)
                     /gene="whiA"
                     /locus_tag="IV250_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAP8767787.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(63188..64186)
                     /locus_tag="IV250_000476"
     CDS             complement(63188..64186)
                     /locus_tag="IV250_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAP8767788.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(64183..65067)
                     /gene="rapZ"
                     /locus_tag="IV250_000477"
     CDS             complement(64183..65067)
                     /gene="rapZ"
                     /locus_tag="IV250_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAP8767789.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(65202..65933)
                     /locus_tag="IV250_000478"
     CDS             complement(65202..65933)
                     /locus_tag="IV250_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAP8767790.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            complement(65937..67202)
                     /locus_tag="IV250_000479"
     CDS             complement(65937..67202)
                     /locus_tag="IV250_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HAP8767791.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(67465..70281)
                     /gene="uvrA"
                     /locus_tag="IV250_000480"
     CDS             complement(67465..70281)
                     /gene="uvrA"
                     /locus_tag="IV250_000480"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAP8767792.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(70291..72285)
                     /gene="uvrB"
                     /locus_tag="IV250_000481"
     CDS             complement(70291..72285)
                     /gene="uvrB"
                     /locus_tag="IV250_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAP8767793.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQVRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            72544..74046
                     /locus_tag="IV250_000482"
     CDS             72544..74046
                     /locus_tag="IV250_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8767794.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            74048..74785
                     /locus_tag="IV250_000483"
     CDS             74048..74785
                     /locus_tag="IV250_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP8767795.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            74791..74949
                     /locus_tag="IV250_000484"
                     /pseudo
     CDS             74791..74949
                     /locus_tag="IV250_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008788611.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding domain-containing protein"
     gene            complement(74956..76149)
                     /locus_tag="IV250_000485"
     CDS             complement(74956..76149)
                     /locus_tag="IV250_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8767796.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDRKYLYKYVDAFGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRANVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVLENDTKKKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQQVMKKRPKAEPFVIDGRGNFLFVNHKGKPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(76228..76434)
                     /locus_tag="IV250_000486"
     CDS             complement(76228..76434)
                     /locus_tag="IV250_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAP8767797.1"
                     /translation="MSNAQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEQVIDKLDAI"
     gene            complement(76427..77569)
                     /locus_tag="IV250_000487"
     CDS             complement(76427..77569)
                     /locus_tag="IV250_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP8767798.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRYPTTDALEIIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CDGVIRRFDLAINDMCGLLDIPVLSEKYRNGGADCRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRGTKAVQAVEELLLTYNPHG
                     LVFYLITDFVQFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDIADVIESEGVFYE"
     gene            78081..78716
                     /locus_tag="IV250_000488"
     CDS             78081..78716
                     /locus_tag="IV250_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8767799.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            78870..78947
                     /locus_tag="IV250_000489"
                     /pseudo
     CDS             78870..78947
                     /locus_tag="IV250_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646598.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            79192..79308
                     /locus_tag="IV250_000490"
     CDS             79192..79308
                     /locus_tag="IV250_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAP8767800.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            79324..>80456
                     /locus_tag="IV250_000491"
     CDS             79324..>80456
                     /locus_tag="IV250_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP8767801.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRY"
BASE COUNT        22584 a        17287 c        14359 g        26226 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaaatagcat attttgaaaa gaaagtctga tgcttagagt agaatttcat aaaaatttac
      181 tatttcataa gtattcacga gaaaaataca aacaaaaaag cggaaataac gctcatccgc
      241 cttcttgttt gtgtttattt cctcttcaaa cgcttgtcta tgattcagac ttttctctat
      301 cgttattttc atctaaaagt tcttgcattc cgataatttc tcgatattga tgaatcttag
      361 tgataccaat gactttgtct acatcaatac taaaacaaat accattacct ggaatatcga
      421 tgttgatgcc ttgaccaatc gcatccatta ctttattcgt caaactatcg ggtactaatg
      481 tcaacacaat atctttttct ggcgaaatgt ccaaacctaa aaatttccct gaatcatgta
      541 caccagaacc tcttccatgt aagatcgttc cgcctgctgc acctgcttct tttgaaaagt
      601 cgataacatc ttcgcccatt cctccgtcaa cgatcgtcac aatcatgtga agattcaatg
      661 ctacaagatc tttcatttgc tttcccccaa tttttgcaat ctttcttttt cgattctgtc
      721 tttttcatct tttcgtttat aaatatatcc tagtactaaa gtggataata ttggtgtcaa
      781 tgcaatcatt gcaaccacac caaacccgtc aatgatcgga tctcttcctt caatttgtgc
      841 agcgattgcc acgctcattg ataaaataaa tgtcgaacac atcgggcccg ttgctactcc
      901 accgttatca aaagccattg ctaaaaagat cttgtccact tttcgtccta aaataaatgc
      961 tagcccgtat cctgggatca agaaataata aagagagaat ccgacgagca aacgccacat
     1021 agacaatcct actgctgcgc cgatcccaat tgaaattacc atcagcatga ttttcgatcg
     1081 gattctcccc tcgctcaatt cttcaacctg tttgatcatt acatgaatcg ccggctcagc
     1141 aaaccctact aagaacccca ttaaaaagcc aaggggaacc agtatataat tgtaaggcaa
     1201 tcccgcaata gcactgccta aatgctgtcc taccggtaca taagccatat tgactccgtg
     1261 caggaaaagg atcaacccaa tagtcgttat caagaaacct ttcataatgt caaagaaacg
     1321 ctttttgcgc atacgaaaac taatgatatt cattaaaaca aataggatta cgataggtgc
     1381 cactgccaaa atgacttcca tggtaacgat ttcgattcca tctatcactt gctgccagaa
     1441 actcattgga agatcacccc caaaatcatc acggcaagaa tcggccctaa cgaagcaata
     1501 ccgaccattc caaaactgtc attttcattt ttcttttgtt tgatcatact agtcatccct
     1561 ccggcaaggg ccaagataaa tggaacagtt actggaccag tagtcactcc ccccgaatca
     1621 aaagcaatcg gcataaattc ggaactggtg aaaaatgagg cgatcaaaac gcctatgtag
     1681 cctatagcca tcaattgata ataggatagc ttgaatacga cccgaagcag cgcaaaggct
     1741 agaaatactc cagtgccgac gctgacgatc ccaatcagca acactctgcc aatctctccg
     1801 tcagatatat catatacttg ttgtcccaaa acttgcacgg aaggttcagc aatcgtgatc
     1861 acgatcccga tagcaaatcc caaactgacc aaaacggtca aacttttctt tttgatcata
     1921 tatttcccta ctagatctcc gacttccatc atagaataat ctgctccaaa caaaaagagc
     1981 gtcattccga tcatcatgat tgccgcacca accagaaatg aagcgaggtc ttctccttct
     2041 aacggtgcaa aaacaaacgt taaaatgaca atcaggacag tcatcggcaa tatagcagca
     2101 atcacttctt taaaattttt tagcatgtat cctcctcctt tactctttaa gtttacctta
     2161 aaatgaaaga gttttcgagc acaaacttaa aacgttatca tttaattttt ctcaacataa
     2221 aataacacac gtcccaaaag tatcttttta cttggaaaaa tgactttgaa ggaacgcgct
     2281 tattgtgttt ttttatttta cagagccgat cattccttga acgaattgct tttgcaaaac
     2341 aaaaaatacg atggcagttg gtaaagtagc gatgactaat ccagccatga tcattccata
     2401 gtctggtgta taggaagcac ccatagcaga taaaacaagc ggaaccgtcc gtttatcagg
     2461 agattgcaaa gctactaagg gccataaata attgttccag ctactcatga atgtgatgat
     2521 tgcagccgcg gcataggtat ttttagccgt cggcatatac actctaaaaa aaatacttag
     2581 ttcttttaga ccgtctaatc gagcggcttc tactagatct tttggaaaag atttaacatt
     2641 ttgtcgaaag aagaagatca aaaaagcagt gctaacagat ggcaggatga caaccaagaa
     2701 actgttgatt cccaaaaccg ttccgttcaa ttgtgaaaac attcgatata aagggatcat
     2761 caatgccgca aaaggaacca tcatcgatag cagtagaata ttgaaaatta ggtcttttcg
     2821 tttactttga taaatctcaa atccatatcc tgccattgac gaaatcagca atgcaaacac
     2881 agtggtcacc aaagcgatca tcgctgaatt ccaaagggaa cgagtaaaat tcagctcatt
     2941 ggaaaacaaa ttttgaaaat tgatgatcaa gttactgcca atctttattt ttcccgatgt
     3001 gatatccacg ggtgtattcg tcattcccaa aaccatccag acaaaaggga aaatggaaat
     3061 aaatgatagg atgcttaatg ctgtatactt taatactgac ccaattgtcg aacgaagttc
     3121 taatgcttgt tttttgacgt tggggcgttc gattgtttct ttttctacca tatttttcat
     3181 cgactttctc ctcctacttt cgtttggatt atcgacaata tcacaataaa ggaaacaata
     3241 acaaaggaaa ctgctgccgc atatccaaaa ttaggcgtga atttataact caaattatag
     3301 atgtattgag atagggtggt cgttgcattt gctggtcccc cgccagtaat attttgtact
     3361 tcatcaaaaa gctgcaatgt cccgatcgtc gaagtaatgg aagtaaacaa aatgattggt
     3421 tttaaatttg ggatcgtcac tttgataaac tgctgccatt tagaagcacc gtcaatagat
     3481 gctgcttcat aaatctccga atcaatattt tgcatgcctg aaaggaaaaa aatcatattg
     3541 tatccagtcc aacgccaagt gatcgaaaga atgatcagaa ctctcgccca aattggatga
     3601 gtcaaccagc caatcggtgt gtcaatcata ccaatgccca acaacaagtt gtttacaagg
     3661 ccagattgag aaaatagact tttcatgatc agagaataac tgaccatcga agtgatacat
     3721 ggaagaaaga tagcggttcg aaacaaccct ttatatttca actttttatc atttaaaagg
     3781 ttggaaataa aaagtgctag aaacaacatg atcggtactt gtacaagtaa atacaggaat
     3841 gtattaaaaa aagctttttt aaaagttgca tctgtcaaca tccgctgata attacctaac
     3901 ccgttaaatg tcatattgac agggggacca ctttgaaaag acgtgatcaa agccaaaacc
     3961 atcggaataa aaaccatgat tgatatcaaa atgactggca agattaaaaa agcatgcccg
     4021 tttttgaata aatctttctg cttcattctt ttccctccaa ttctatcatt gattatctac
     4081 atgaaaagac aagaaagaca atatctgtaa ggcagacgtg atcttttttg tcttttcgtt
     4141 tgtctgatga aaaagcgaag aaattcactt ttttaccacc gatcaattag ccaattgtgc
     4201 ttctacttgt ttttgtgtat ctgctagtac cttgtctgtc tcttctccat tgatgattcg
     4261 atgaagagct tcagctacga ctgcttcaat tgcataggtc tcatgtccgt aattcacttc
     4321 tggtatttca gatgtccatg ttgaaaaatc gtcaaagatc ttttgaccac tgtaaaattc
     4381 agaagcttct tgataagctt cttgttcatt cgcgctaagc atcgtagata ctagtccgat
     4441 ttctttcgca agagtgccca tcagttcttc attcgaagca aacgttgctt ctaggaaatc
     4501 tttcgctgaa tcttctccag ctactccttt gatcacatac cagccagcgc cgccaaggtt
     4561 agaagcttgc gctttttgca gattttctgg aagtgcaggt attggtgcaa tcttccattt
     4621 tccagattgg ttttctgctc cttggatcgt tgaagaatac caagcgccag ttgggctaga
     4681 agctactgct cctgattgta tcgcagttac tccagcattc cagtcagagg tttgttcgac
     4741 tagatcttct ttcaaaagag tggcaaacaa ttcaagacca tactttaaag atgcattgtc
     4801 ttggatcgtg actgtctccc catcttcaga tgtataccat tctcctgcct gttgcataat
     4861 catccttact cgaccaaggt cagaaggatt gacttcagcg attttctttc ccgtcttttc
     4921 tttgacatct cgcgctactt gaatatactc gtcccacttc agattctcca tatcctcttc
     4981 tgtataacct gcttctgcca tcagatccgt acgatagaag tttgctgtta cgcccgaatc
     5041 gaaaggaaca ccgtatattt tatctcctac tttgtttacc gcaaatttat acggtgcaaa
     5101 atgatcttcc tttacaatag cagacaaatt agcgaatgca tctggataag aagtcaaata
     5161 gccttgtatc ctataatctt caatcaagac gatattgggc aatccttctg tattcccgct
     5221 cgctaaagcg gtgttcagtt tctgaacgat atcatcctga gacatcgtaa caacttctat
     5281 ctctgtttct tcgttctcat aaactttttt tgcttcattc actgctttga tattgaatgt
     5341 ctcatcccac gcccagacag tgactttttc ttctttgtct gctgtactac tagcacttcc
     5401 gcatcctgtt aagaaacctg atgctgccgc taaacaaacg actccagcaa ttaactttct
     5461 gatgttcatt tttatccgct ccttttccat ccattagttg tttacgtttg ctacattcat
     5521 cataccaaaa tttgaaaacg gtttctttat tattcagata cttcaaaaat ttctcctaaa
     5581 ataataaaaa aatacaaatc aactatgaca aaaggaataa tcttagcatc tagaccaaag
     5641 atcgaaatgg tgaggtttct ttaaaataaa aatatccttg cattttctcc tagaaataag
     5701 tatttttaaa ttacaagact attcttaagg cgggtaaaaa aatgccaata cgcgtaattc
     5761 ttatcgatga tgaacagacg attttagatg gaatgaaaca gttgatcaat tggtctgagc
     5821 acgggtttca attaataggg actttttcca gtccgctgaa agcgcttgat ttttctttgg
     5881 tacatgcaat cgatatcgtg gtcacagatg taatgatgcc tgaattaaat ggaatcgaat
     5941 tatcgcggct attaaaacag caacaacctg aaacacagat ccttatcctc tctagctatg
     6001 atgaatttga tatggttaaa gattctttta aagaaggcgt cgctgattat cttttaaagc
     6061 ctcgattgag tcctgatttt tttctaaatt ccttaactgc cgtttcacaa aaaatcaaaa
     6121 aaaagcccaa caaaagccca atcaccaatc gttatgagca gatctgcgag aatctttcac
     6181 agcagattgc tggacaaact cgtccattgc ttgaccctct tctttttgaa cacaccttgt
     6241 ttttctgtta tataccgaaa aacggccatt cagttcaaaa caaggctcta aacaaaaaca
     6301 gtcgatcacc cattcattga tggatagcga cgattcgttt actatttatc ctgtttctac
     6361 cttttcggaa gaatccggct atgtaatcaa tactgattcc aaagatacac ttaggaagtt
     6421 tgttcaaaag tttcgtcaag aagtttttcc aggtgatttt tttgttgttt ctgattcttt
     6481 tgcaatccaa gatatcaatg aaatattcca agagatcaga aattgcacca aaggacaacg
     6541 tttctatcat aagggaaaga accttgctta tcagcatgaa cttcttccgt tattaaccaa
     6601 ccacgagcag caagcaagta gttatttaac aaaaatcgtt cataaagact acgctttagc
     6661 tgtcaaagaa cttgagaatt tcttcacgac gatattggaa cagcctattg atccagctta
     6721 tttgaaacat gaggcaatca atcgtttcta tgcattactc aacgctttgg ctgacgagca
     6781 tgttgggaat gaagaacttt ctttgctcaa gatcgccctt ccttctttat tagccgatgc
     6841 tgagtatttc gaagattttt ctattcttgt caaagaaaca cttgagaaac tcacccactt
     6901 ttctgcaaat gcaaaaaaag acaatttcga tatcttgact ttgatctacg agttcgtcca
     6961 ggaaaattat gagaaagaaa tcaatttaca acttttatcc gaaaaatatc atttttcata
     7021 tagctatctc tcagccatct ttactgaaaa atttggtatc aacttttcaa aatatctaaa
     7081 aaaagtccgt atcagtaaag ccaagctctt tttgacggaa acacggctta ctttgacgga
     7141 aatctgtgag aaagtcggct ataccgaact gggttatttc tccagagttt ttaaagaaga
     7201 gacaggaatg acaccttctc aataccgtaa aggaactttt gtaaaatgag aaaaaaaatc
     7261 aaagagcatg aactctttgc caaacttctg ttcattttgt tcatcggtct ttttctgcaa
     7321 gccatcgtca tttctttgtt tatttatcat cgatcaaggg atgcctatat ccagctgttc
     7381 aatcaatcga atgatgtggt actaaaaaag attcaatctg aatttgaaag tttgaatgat
     7441 acaatcgaga atacattagc cgcttttgat tctaatccag ctgtcaaaag ctatttttcc
     7501 aatgatccag cccaacatat ggaacagctt cagcaactaa gaacaatcca aaaaatgaat
     7561 gactctcttt caaaaatcca tccaatgatc gattacgatg tattgatctt tggagaaaat
     7621 ggaagaactt ttgtcggaaa cgacatgctc actgccgtat ctgccgattc tttttttcaa
     7681 tctgcgattg ctcagagagt gaatgaacgt gctgctgata cacaaatgct ttttgcacac
     7741 cacggattga ctcttcgcga taagaaagca cccagcgtct tctttgtcaa aaaactgaaa
     7801 aatacattga atcacgttta cggatatgct gttttatcga tctcatccaa gcaactagcg
     7861 aatctttttc aatctgtagt aaatcctgag atatcgaaga tctccttcat gaacgaagaa
     7921 cagacgatca tcgcatcaaa tgaagaaaat acaattggga gccgatcaat gattttcaaa
     7981 aaactaacag taggtgaaac aacgatttca aatggacagc agttgacccg tcttcctctg
     8041 taccgccagg attgtacatt actcagtcaa gtcgatcttc attctttatc taaccaaatg
     8101 ggcgtgattt ttccgattat tttgtataat atcgtgacac ttgcttttgc tggctcattg
     8161 gtatttatgt atttggatcg acacacaaaa tcgatctacc gcttgattga ttcgctaaaa
     8221 cggctagagg aagatggctt aaaagctact gttcctgtac aaggaagcca tgaagtccaa
     8281 ttgctaggga atacgatcaa tcaattgcta agtaatgctc agcatcatta tgaacagacg
     8341 atccaagacg aaaagaagaa acgttcgctt gaaatccaaa ctatgcaagc acagatacag
     8401 ccccatttta tttataatac gctgactagc ttgaaatttt tgatttggca gaaaaaaaca
     8461 gatgaagcag ttcaaggaat cgacagtttc attactttac taagaagtac gattgggaaa
     8521 aaagaagaag tgattccagt caaggaagaa atcaaaagcg tacaaagtta catcgatctt
     8581 ttgagtatgc gatatggcga aagaatctcg gctaaaataa tgattcctga tgaactgcag
     8641 tcattatcta tgccgaatat gatccttcaa ccaattatcg aaaacgccta tcttcatgct
     8701 tttcacaata aaacgcaagg attcatcacg gtgtacggca aaacaaaaaa tcagcttctt
     8761 ttattcgaag tgattgataa cggcgatggc ttcgatactg aaaataccaa acaaaaaaat
     8821 gatcattttt caggaatcgg catcagccat gtagatgagc ggatacgcct aatgtatggc
     8881 gatgcatatg gcttaacgat ccaatcagtg attgggacag gaacgactgt aacgatttgt
     8941 ctaccgcttc ttcctaaaaa tcaatcagaa tatcaaaaat aaagaatagc caagcgctga
     9001 tttttttatt ttaagttaaa taaatcaatc agtattcttg actttcatct acgcgtctct
     9061 tctactgtca gcactggata acagcccaat atttgacttt ctcctccgag catcttgatt
     9121 tcttggagcg cattctctac taatgtcatt ggacggtcca acaacaaatc gatcacgaaa
     9181 aaatattctc ccaagctagt tttcaatggt cgggattcaa tcttacttaa gttgatttcc
     9241 cgccagccaa aagctgccag cattttatga agcatccctg gccgattagc aggtagtgtc
     9301 aagatgacac tcatttttac tggagctgac tgctggctgg tcatctttcg gtcaccgacg
     9361 atccaaaaac gtgtctggtt caactcatta tcttggatat tctcagctaa gatctccaat
     9421 ccaacatgct gtgctgtttc taatgaagca atcgctgctg cgtcttcttt cggatgttct
     9481 gctacataca ttgctgcagc agttgttgat tctgttgcta ctaaaggaac attaggataa
     9541 tgcgtttcta aaaactgttg tgattgtgct agtgcttgtg gatgtgaaag gatttttgta
     9601 atctttgttg ctggattgcc taaaagctgc tgcttgatag gaagaacgat ttcactttta
     9661 acttcgactt ctggatgttt ggaaagaaga tcgattgtgt gatgaacagc tccttccaat
     9721 gaattctcta caggaacgac agccagatcg acttgtttgc gaaacaaagc gcgcaaacaa
     9781 acagggatgg aagcatacgc agtcaattgt tctgtaggaa atagctgaca agccgcctga
     9841 taagtgaatg aactttctgg ccctagatag ctgacgttca tatgcttttg atcctttcta
     9901 taatggtatc gatgatcttt tctggtgttt gattggtcgt gttgattcga tgtgtcgcac
     9961 tttcttcata ccaagaaagt cgttgtgcca ataatgcttt tatttcttct cttgtttttc
    10021 caagagctaa aggtcgaata gacgtttcat cttgttggat acgatcaact agacagtccg
    10081 cttctgcctc taagtaaaga acaatcggct gtgtttttaa aaaacgccga ttttctgcac
    10141 tttggacaat ccctccacca gtcgcgataa ttttgtcttc atttgacagc tttcttaaaa
    10201 tatctgactc cacttttcga aagctttttt caccgtaata gtgaaaatat tcggcaatcg
    10261 acaattggat atgttcctca atctttgtat ctaaatctac atactctttt tgtaaacatt
    10321 gagctaaccc tttaccgatt gttgtttttc cagcgcccat gaaaccaatc agcaaaatgc
    10381 tagccatgag cttctcctcc tgacacaagt tctgctaaat cgtcaaaaaa tgccggataa
    10441 gagattgaaa cagcttcagc tccttctagt tcgcaaggtc catcagctat caaagcagct
    10501 acttgcagca tcataccgat tcgatgatcg cctctgctgg acacttttgc gccatgcaga
    10561 ggtgtcggcc catggatgat cagaccgtct gctgtcgctt ttatctttgc tcccattttc
    10621 tgtaattctt ctgccaccgc atcaattctg ttggtttctt ttacttttaa ttcttctgcg
    10681 tctttgatga tcgtgattcc ttctgcttga gtagccacta aagctagaat gggcaattca
    10741 tcaatcagac gcggaatgat ttctccacca atcgttgctt tcttcaaatg actcgtcttg
    10801 acacttaaat cagccgattg gttctgttca ttgtgattcg tctgggtaat cgctgcgccc
    10861 atctcctcca acacatctaa gataccagtt cttgttggat tgaccccgac attttttaac
    10921 aacagttggc tttccggaac aagaagtcct gctgctagaa agaaagcagc tgatgaaata
    10981 tcacctggaa cagttatctg ttgaccagtt aatttttgcg gtccggtcac tatgatcgtt
    11041 ttatcttcca ctgtgatcct tccgccaaat tgacgaatca tttcttccgt atggttacgg
    11101 gaacgttctt tttcaacgat tacggatgtg ccttctgctt gtaatgccgc aaacagcaaa
    11161 gcagatttga cttgcgcact ggctacaggc atattgtatc ggataggtga taaagagcgg
    11221 gttcctgtca cagtgattgg tagatactgg tcattttctt ctccttgcaa gtcagcgccc
    11281 atctcgcgta aaggttccat cactcgcccc attggacgtt tagacaacga agcatccccg
    11341 aacagagtag tcgtaaaagg ctgtccagcc aaaataccca tcaataaacg tatggtcgta
    11401 ccggagtttc ccatgtcaat tgattcggtt ggttggacaa atgagtggct accgcgacca
    11461 tgaatcttga tcacctcttc ctcttcttcg atctcgacac ctaattgttg gaaggcatgt
    11521 aaagtactca gacaatcttc tgcgcgcaaa aaattttgta tagtcgtcgt tccttcagca
    11581 attgctccaa acatgatgct gcgatgggaa attgatttat ctgctggtat cctaacagtc
    11641 cctcttaatc catgtatttg ttgtaataat tgcatttatc cagcctccta tttgatccag
    11701 caatgataac tggtattaga cataatgatt ctttttgctt gtaacaaatc ctgttggttt
    11761 ttaaaactga tttgcagtat tcctaaaata tcttccctgg tctcttggat cttcaaatta
    11821 atcaaagaaa ctcccgcttt tccgatcagt ccagttactt ctgcaataac gccaggttca
    11881 tctggcacat cgataaataa atcaaaaaag gcaggaatcg cgccttgctt atggacaggc
    11941 aactggtctc gactttcttt tgcccgattg aaaaaagaaa agatttccga ttggttctca
    12001 gtcatgatcc attctgctat ttgtgtcatt tcttgttgcc aatctgccaa tcgttccaat
    12061 aatatctgct tattgctcaa caaaatatct gtccacatga caggatctga ggaagcgatc
    12121 ctagtgatat ctcgaaagcc tcctgctgcc agttgtttgg ctcgcggata ctcttgattg
    12181 aattgttctg cctgattgac taagccagct gccagaatat gtggaaaatg acttagcatt
    12241 cctgttatac ggtcatgttc tcttgctgta agagttacat attttgcttt ggttccgcaa
    12301 aatagttctt gcagtttttc aacatctttt ttcgtctttt ccaaaggtgt aaagatgtaa
    12361 taggcatttt caaatagttt agcatctgct gcctgtatac ctgatttatg agaaccagcc
    12421 attggatgtc caccgacaaa ccgaagattt ttctgctcag cggatgtcac gacttgctgc
    12481 ttcgtactgc tgacatccgt aatgatgacg tgatcagaca agttgattct ttcaaggaca
    12541 tcaagataag ctaaggttgt acggacggga acggctaata agataacgtc tgcttgttct
    12601 gcacctgttt caaaatcact tgcaacgtaa tcaacgatct gctgttctac tgcttgctca
    12661 cgggtttttg actgactatc ccaaccaact actatcagtt ctggattggt ctgacgaata
    12721 caaagtgcca tagaagcacc aatcagtcca agccccacga taaaaacatg ttgtgccatc
    12781 ttatttgccc cctttttagt aatgtttcaa gtagtcacgg tactccttca ctgcagcttt
    12841 caactcaaca agcgaatcac ttgggaattt tgacaatagc tctgtcgcta ccacagtggc
    12901 tacaacattt tccgcaacga cgctagctgc aggaactgct gtactatccg aacgttctac
    12961 acttgctttg taaggttctt ttgtatcgat atcaacactt tgaagtggct tgtacaatgt
    13021 aggaatgggt ttcatgaccc cgcgaatgac aagtggttcg ccattggtca ttccgccttc
    13081 gaagccgcct agattattgg tacgtcggaa atagcctctt tcttcttccc ataggatctc
    13141 atccatcact tctgagcctg gtaatgaacc agaagtgaat ccgaccccaa attctacgcc
    13201 tttaaaggca ttgatactaa ccacagcttg tgcgattttg gcatctagct tcgtgtccca
    13261 ttgtacgtag cttcctaatc cagctggaac gcctccaacg agcacttcta caattcctcc
    13321 gatcgtatct ccatttttct ttgtctgatc gatcaaatct tttattcttt gctcaactgc
    13381 tggatcaaca actcgtactt ctgacgcttc agaaatttct ttgatttctt gaatcgagat
    13441 atttccaggt atttctgctt ggattccacc aagacaaatc acgtgtcctg ctacgatgat
    13501 gtcaagttct tccagaattt tcttggcaat tgcgccgatt gctacacgca tggtcgtctc
    13561 tcttgcagag gaccgttcaa gcacattccg aagatctcga tgatggtatt tcataccgcc
    13621 taccaaatcc gcgtgtcctg gacgcggacg tgcaactcgc cgcagtgatt ggtctttttc
    13681 aggcacttct gctacagaca tgacagttgt ccaatttttc caatctttat tttctaccac
    13741 taaagtgaca ggtgaaccaa ttgttttgcc gtgacggatg ccagacgtga tccgaacacg
    13801 atctttttcg atcttcatcc ttcctcctct gccgtaacca gcttgtctgc gagcgagttc
    13861 gtgattgatt tcttcttctg acagaggtaa tcctgctggt agtccttcaa taatggctgt
    13921 taattctggt ccatgggatt ctcccgcagt catataacgc atcttacagt cctcctaagt
    13981 aatctttgaa tgtctctgtg ggaacaggaa cgatttttgc ttgcccaatc ttttctagca
    14041 agatgatatt caattggctt cctcttgctt tcttatcatg ggttatcgcg tggaataatt
    14101 catctggatg acttagatcg tacgtaatcg gcagatggaa tttttgaagt atcctttcta
    14161 aagcagctgt cgatccagtt ggagacaagc cgattttttc tgcatgagaa gtgatggcta
    14221 ccattccaat agctactcct tctccatgac tgactacccc atatcctgct gtattttcaa
    14281 ttgcatggcc aattgtatga ccaaaattta aaataagtcg atttccttgg tcaaagacat
    14341 cttcttccac tacttttcgt ttgatttcca acgtagcagt aatgattgga acagcatgtt
    14401 ccagaagatc tttttcgtct ttcagctcgt tcagctgttt ccaaagcgtt gcatctgcta
    14461 tagctgctga tttgatgatt tcagcgattc cttctcgtaa acgacgtacc ggcaaagttt
    14521 ttagaacctc aggatcaatc aatacaccag ctggctggct aaatgttccg acaaggtttt
    14581 tcgctttgtt tgtattgact gctgtcttcc cgccaatact agaatctact tgtgcaagta
    14641 gcgtagtcgg aatttgaacg aaatggatcc cacgcatgaa cgtcgaggcc acgaatgctg
    14701 ctagatcacc caccattcct ccaccaagtg caagtatccc atcgctgcgg gtaaaatcat
    14761 tatctgctaa aaaatcgtat aactcagctg cttgttctaa agatttactg gtctcacccg
    14821 ccggtacgat tgaaaggact ggttcaaaat tagcttgtct caattggaac atgatttttt
    14881 ctgcatatag tggagcaacg tttgtatcag tgatcacagc tactttttga ggtctccaaa
    14941 tcgtttgaag ccatgatcca gcctgttcta gtgcgccttt ttcaatatga atatcgtatt
    15001 tttggttatc aacatgtaca gtaagcattc gtttcacttc tttcttttaa aaagctggaa
    15061 ttaatcgagt gtctgaatct tttccattgc ttctttcatg atcgcaactt ctttcatcat
    15121 gcgtgagtaa gtcttttcat tcaacgattg gggaccgtct gaccaagcat ttgcaggatc
    15181 aggatggatc tctacgatca tgccatctgc tccgcttgcc acacccgcac gagccattgg
    15241 tggtacaagt tcccaaattc ctgtaccatg acttggatca acaatcacag ggaaatgagt
    15301 taattttttc atcaatggga cagcacttaa atcaaacgta ttacgtgtcg ctgtttcgta
    15361 agtccgaatc ccacgttcga taaaaatgac aggagatttg tcttcgactg caatgtattc
    15421 tgcagcatta agccattcat tgatcgtacc ggaaattcca cgtttcaatc caacaggttt
    15481 acctgtttta ccgacagcag atagcagttt aaaattttgc atattacgtg cgccaatctg
    15541 taggatatcg ctgtactcag ccaccatatc gatatgccct tcatccatca cttcagtaat
    15601 cactttcatt ccaaattcat cagccgcctg acggatgtat ttcaatcctt cttcttctaa
    15661 accttggaaa gcatatggag aagtacgtgg tttgaaggca ccacctcgca gaatttttgc
    15721 gcccccggct ttggccattc gcgcacattc tctgatttgg tctaatcctt cgatagaaca
    15781 aggaccagcc atcgtcacga aactgccgtc accaattttt actccgtcta catccacgac
    15841 tgtattgcca ggatggaatt ctcgagaagt caatttgtat gtgtttaaaa tacgcacagc
    15901 attttctact ccttcatagc gtgtaaatgc tccttcttgt aaacttcgcg tatcaccttt
    15961 tagtccaatc actactcgtt ctgttccttg atcgattgag actttgaatc cttctttttc
    16021 gatatgatcg acgacttttt ttatttgttc ttccgttgct tcttttttca taattactat
    16081 cattgttttt cccgctttct tttgtttact tatctatctt ggctgatttt ctctactttt
    16141 tcaaggtggt tttacattaa ttcctactaa atgaccgtgt cctgttgttc tcattttcgc
    16201 tccttctttc ctacttatga cccagataat caacaaaaaa agatgtccct gtaggaaatt
    16261 gcctctacag tgaacatctt caacgagcct taaaaagcgc tgcaaaaata taaaatagcc
    16321 actatagatg aatttctcta cgtggctgta agtcgtgttt aaaaatcttt agccatcata
    16381 gatacaaacc tggtttgttt gtacccacga agtcatgaat acaaacctat ctaaaatagc
    16441 taaagtaata cgtattcagt tttgtaagtt ttctagattt cataaaaaat cgctccaaac
    16501 tgatgtaaga attgtaccta gtatagacat aaaaaaatcg actgtcaatc atttttatga
    16561 aacttttttt aattttctga tagttaactt tgtttttcag aaaaataaca attggtttta
    16621 ttgaagaaaa tagtcatgaa caaaagtatc tgagaacttt tttactctct tgttcatgac
    16681 atatagaata aagcagcttt gtcttgtaaa ctccgttttt caaataataa tctaaagatt
    16741 gctactgaat aataaagccg atttcatggt cctcttcgct gctgattcgg taaggtgact
    16801 caacatccgc tccccagcta ttgatacccc cgactcctct tacttttgca aaaatcgtaa
    16861 gaaccgttcg tctttctggc ggtagttctt ctatatgtgt tgcctgttcc agttcttctg
    16921 ctttgaatgg taaagcggaa aaagcaaatg gcgtatcctg ctgttcaaat cgcaacgaaa
    16981 aatcttcttg atctttatcc gtattatcca acgtttgatt tcttgttagt tctacccatt
    17041 tcgtttccat atgcatgccg cattcttggg gaacaaggta tggggaaacg gggagtcctg
    17101 ccactttata aataccttcg acaccaccag ccatccgatc cggatatgtt tcccctgata
    17161 ggccttcata ctcaaaaaat tttgctttcg ttggcataat gaaccttaaa cccaatgcgg
    17221 gtaattccgg taatcctttt tgtccataat aatgcatatt gacaagcatt ttaccctctc
    17281 cagcgatttg gtaggaaatt gtcacttggg tttttggtac tgtgttgatt tccagtgtaa
    17341 aaatgatttc aaccgtttca gcgtattcat tacttgaata ttgattattt actggagcaa
    17401 tcggcaaatc gatcttttta ttgtccacca gtacttctat ttctttgact tttggataca
    17461 atccggctcc taaccacata ccggatttaa atccaaatcc gctacctctg tcattatcgg
    17521 tcgttgctcg ccaaaaaact ggaagtgttt cccggtatag ccattctttt ccgtttttat
    17581 tgagcgattc cagtccgcct cttgtataag aaaaaatata atgcgtattc cccgatttga
    17641 cacctaatgt acagtcgccg aagacgattg caactttttc cgtatccata tgacttttat
    17701 ttactgtatt tttcataata atatcttacc tcctgcatgg cgggtttttc ttgtccatct
    17761 gcaaaaacga tcccattccc tgagaattca tagtcacttg gtttatcgtc aaagtcaccg
    17821 ccatagcgca tcacttctct gcctgtgatt ggatcagtca cttggatcgc ctgatcttta
    17881 aaatcccata tatagcctcc ttggtacatt tcataacgat caagcaacgt catgtaagaa
    17941 tccattccac ctaacgaatt ccccatgtca tgcatatatt cacataagat gaacggtttt
    18001 tccgctttat tttcaaggta gtcaatgatc tcttctggtg gcgcatacat gcagcttttt
    18061 acatctgaaa tcgaatcttc gaatgctgga tttggaaaat tcccttcata atggacaagt
    18121 cgatcggggt ctactttttt gaagtaatcg ttcatttgtc gaatgttttc ttccgcataa
    18181 gattcatttc caagagacca aaaaagaata cttggatggt tttttaatgt ttcgaaattc
    18241 gtttttgctc gatcaagaac agccgcctgc cactgaggta ctgcacctgg tacgttgtaa
    18301 gaaggctcaa atgcgcccat tttttgccat gagccatggg attcgaggtt cgtttccgcc
    18361 atcacatgga ttcctgcttg gtcgcataaa taataccaag tgatctggtt ggggtaatga
    18421 gatgtccgta cgccgttgat ctttgcattt tttatgatag ccaaatcttt ttccatatct
    18481 tctatagtga tggcgcgtcc ttttttcgga tgccactcat gtcgatttac cccgttgata
    18541 atcatccgtt ttccattgaa gtagatgatt ttatctttga tttcgatttt acgaaaaccg
    18601 aaggaatagt caatatactc cgttactctt ttttcttcgt ctatcgtctc caattcaagc
    18661 ttatataaat tgggctgaaa atgcccccac agtgccacat tttcaaatcc atcgatgcga
    18721 atcgtcattt gctcttttaa cggatattgc tgttgataga tttccttccc atcaggatct
    18781 agtatcgcta cattcagctg actgtttttg gctacttgct caaaagacaa atctacttca
    18841 agtttgccat tttgacattt gtcatctaag cgaggtttga tccaaagatc ttctaaatgg
    18901 ttttgtcctc gagcaactaa gaagacatct ctgaagatcc caaaaaatcg gaaaaagtcc
    18961 tgatcttcca agaatgcagc tgtacttctt ttgtaaacct ccacagctag aatattttct
    19021 tcttccaaat aaggtgttaa atcaaattca gacggagtga acgaatcttc ggcatacccg
    19081 ataaaatgac cattcaacca aataaacatt gcttgttcta caccagcaaa atgaatcgag
    19141 atttgttttc cctttagacc ttcttctaat cgaaaagtcg tcaaatacga accaaccgga
    19201 ttgtctttag cagctgaaaa gatcccttcc gcctctttct cagggcttgt cgtgaaaggg
    19261 ggacggcgaa atacatgtcc ctcccacgga tacatcgtat tgatgtaatg gatcttgtca
    19321 taatttgcta attcaatatg actaggaaca gtgattttat caaaagaaca ggcatcaaaa
    19381 tcttttttat aaaatgtttt tggtcgtacc gaaggatttg gcgaataaca aaaacgccat
    19441 gatccattta aagactgttt aagggaggat gtggtttcta cttcttccct tgttgcataa
    19501 caaacatgat cgctatgtgc atttgattgg ttcactctaa aaacttgtgg atcatctaac
    19561 cattttatat ttgctttcat acttttttcc ttctctctca taactttttg aaatgaagca
    19621 gttctgtttg gaaatcacct tgaaaatcgg ggaagtaaag tccaactgtc atcaattcgg
    19681 cccccgaaaa tatccgcttc gaggtctgtt cctgataaag tgcctcatca tctaacccca
    19741 tcagatatac ttcatggaac gctggttgag ctgaagctag tatcctccct aaaaacacaa
    19801 tagcttcatc ttgtttagga gacacaaaca tccatgcggc aatattttct tcaaaaggac
    19861 tcgccaaacg ataataatgg ccatactgga tcaccggacg aatcgtttga taggatataa
    19921 tttggtcttt tacttgcttt tcttcttcag gagacagctt tgtcaaatcc agttcataac
    19981 caaaaacagc gctcatagct gtttctcctc ttgttttcaa cgaagtagat ctgtcggtct
    20041 gatgatttgg tacagctgat acatgagctg tgatggacga aatcggataa gcaaggcttg
    20101 ttccatattg gattttcata cgctccactg catccgtgtt atcacttgtc catgactgcg
    20161 gcatataata aataaacccg gcatcaaacc gtccacctcc accagaacag ccttcccata
    20221 agatttccgg ataatcagtt gtcaaatgtt caagtagttc ataaagcccg agcatataac
    20281 gatgcgctac ttctccttgt ctctgtgggt caagggtaat cgaatagaca tctgaaagcg
    20341 aacggttcat atcccatttg atgtagtcaa gaggaatcgt atctaaaatc tttctcattt
    20401 gtttctcaat cgtctgccga acatctaacc ttgtaaaatc cagtacatgt tgtgatctgg
    20461 aagcagaagg cattctacct ggctcctgca taagaaaatc aggatgtgtt cgatacagtt
    20521 cagaatcaac cgagatcatt tcaggttcaa accataaacc aaatttcaat cctttgctat
    20581 gagcataatc tgctatttca cgtaatccat ttgtcaactt gccttcatac tcgaaccagt
    20641 ctcctaatga agaattgtcc gcatctcttt ttccaaacca gccatcatct aatacgaaca
    20701 tttcgatgcc tagctccttt gcttcatcaa tgatcgcctt gattttttca ctattgaagt
    20761 caaagtaagt ggcttcccag ttattgacca gaatcggccg ttcagcaaat tgatgacgac
    20821 ctcttgcgat tctttcacgt agcaactcat ggtggatctg actcattttg ttcaatccct
    20881 gacttgagta agaaaggatc atttcaggtg tttgaaaaga ctggcctgca ttcaattccc
    20941 acgaaaaacg atggctgttg atcccaccga cgactctcaa ttgattgatc tgatcttttt
    21001 caagttcgaa agaatggttg ccggaataaa caaattgaag tccgatcgct tctccagtat
    21061 tttctgtcgt atgatgatga actaaagcaa taaagttatt catatggtga ctgctcgttc
    21121 ctcgacggct ttcaaatact tttgttccag ataaaacaga ttgccgtgaa atctgtcttt
    21181 cattgacatg tgctcctggc aaagcaatga cttcatcaaa tcgcctagta tgggcaaagt
    21241 ctagctggaa actagccgct ttttctagtt tgattgtttc tccggtttca tttcttatct
    21301 ttacagatcg tgtgatcact gctctatcag taaatatggt atagaacaaa tcgaaataaa
    21361 tcgctgtttc tctatcctta agtgaaatca ccaacgtttg tgcctctgac ttgtccatca
    21421 cataagtttg aggcaagcct tcgatctctg gcttcccatc tgtaatataa tggctgtcat
    21481 atctcagatc cagtaagtct gatccattct tgcgctgaat gatcgatgcg ggcgttcgaa
    21541 agtccatgct gccatgagaa gagtattctt gcggtagtgt atctttcgac agagaccggt
    21601 caggatttaa gggaacattt cctgaaaatc cacgatctct tcggggataa tttttattgt
    21661 ctttgtactg cttgactggt ttgccaaaat aaatatgcgc caatacattg ctttcttcta
    21721 tctttatcag ataagaaaga aatgtatttc gtaaatggaa gactcggctc ttttcatcaa
    21781 ataagatgaa atttttatct gtttttctat gcgtaatatc catcatttca ctcccaacaa
    21841 aacggaaacc gtttactttt ttttataatt cctgtatact gtttactaaa caattttgtc
    21901 aataaaaatt ttttattttt ttgaggagtg tgtaaaaatg gttggaatta gagatgtcgc
    21961 acgaaaggca aatgtctctc ctgctacagt ttcccgtgtg ttgaataacg atcagacgat
    22021 ccatgtggct cagactactc ggcaacggat agtagaggca gctgctgcgt tggattatga
    22081 catcagtact cgtaaatata tcaaaaagag aatgccttcg attggtgtga tctctacgat
    22141 cggcaaagcg agcgaagaac aggatatcta ttacaaacat ttgcgagtag ggatggaaga
    22201 agaagctcga cgtctgcatt tagggatgaa tcgaatctat aatctatctg ataatcctaa
    22261 acaatgggaa gatctagatc agttaggtgc ggtgatcgtt gttgggactg ttacgaaaga
    22321 atcgattacc gccttgttaa aacaaaacat gcaaatagtc gtcgtggata accctgacat
    22381 ccagcaagag gtcgatatgg tgtatgcaga tcttgaacgg atgactaaaa ccgtactgga
    22441 attgttctta gaaaatggtc ataaaagaat agcctatatc gggggatata aagtcgatat
    22501 ggatgaattc ggtgggaagc agatttctat aaatgaaaag aggatgcgtg cctatcagca
    22561 ttttatgatc gatcatcact tagaaaaata tatgtcttat catctaggtg gctggacaga
    22621 aaaacaaggt gaggtacttg cgactaatct attagaaaat gaatcccctc ttcctactgc
    22681 cattcttgtc gggagcgatc caatggcaat tggtgtctat cgggcattga aacaaaaaaa
    22741 tatccaaatt ggagaagaga ttgttgtagc aagctttgat aatatcgaga caatcgcaaa
    22801 cctctcccct ggcttgacca gtgttcaaat caatgcgaaa tcaattgggc aagcagctgt
    22861 tcgaatggca ttggagcgaa ttgacggtat acgggacgaa ccaatcattt tgacttatcc
    22921 tgcacgattg attgttaggg aaagttttat cccaagaaca aaaaggtatt gaaagagagg
    22981 ttgtgaaaag ccgcttcgtt tcttcatact caaaacgaaa cggctttttc acaacctctt
    23041 acttatactt attcacaaaa tccaatgtct gctggtaaca attttgcgat tcttttagcg
    23101 tgtaatcaaa ttggtattca tgggtaatct ctttttttgt atctgtataa aacaaggatt
    23161 gcacgggtac ctttaattct gtcaaccgat tttctaatgc ttgaccttgc tcctggaatg
    23221 agtatgcatt tccgtcagtt atatacgttg gcggaaattc cttagaaaca tggtcagcca
    23281 cactagcttc tttcaattgc tcactcgttt tccaatcttt tgttccaatc aacgaccacg
    23341 caacggtttt gacgaaaaac ttcataaatc gatcatccga atgctgctgt gctgttagct
    23401 tcaaatcaac aggtccgcaa taagaaatgg ttcctttcag actatctttt ggaagtagct
    23461 gtttgatttt catttgttta gcatacgcat cattcgtttg tatagctgca tattgcaatg
    23521 cgatttgtga acctgcacta tctccaccaa aaaataattg ttccatgtct agcaatttgt
    23581 cgtctttttc ttcagctaat aaatctttga tcagttcatt cacttgaaga acttgattag
    23641 ggtattctga atcaggcgct aattcataat tcatcgcaac cactgcgatg ttggcgtctg
    23701 aaactaattt tgtcgcaaat tctttgacac ccgatttatc tccgccgacg aatcctccac
    23761 catgtaccca aaatagaaca ggaacaggcc ccttgctgtt ttttggatag tagatgtcat
    23821 aagtattctc tttatacttg gactcatacg tcaaatctgt ttttttcgcc acgttttttt
    23881 ctgcttgatc gaacgttttt ttatcagtga tttgaacagt atttgaaaac atatggttga
    23941 tcaagaatgc tccaggtctg ggggaaagct ggaaggcaag aaagattgca aacgcgatcc
    24001 caagaatgat tccgaagaac catccgataa atttaagaat tttctttttc ataaggctcc
    24061 ctcagtagtt agtattaagc attctgatta taccatagtg ttatttccta tcaagctctc
    24121 ctagatgatt taatgatttt ccccaaactt cttctgcaat gcttttgaca aaagaaattt
    24181 tttccattgt tcttcaaaca aaacattttc ttcttctgtt gaagcaaatc cacattgagg
    24241 acttaagcac aaacgatcca aaggcagata tcgtgtcgct tcgtagatcc ggtcaatcac
    24301 ttgctggtgc tcttccattt cgccattttt cgttacagtc aaaatctaag acaactaatt
    24361 tatcttcaga gactttctct aaaagaacaa aactcccagc acgttcacta tcgtattcta
    24421 aataataggc acggacattt tcttgagtaa aataatgggt ctgctacgtc gtcatccccc
    24481 ctgatgccgc caaaatcgaa taataatttc cgcgtcaaac atgtatattt agtgttgaat
    24541 catttgagaa tatgaaaata aaaatctttt tgatcaatca gttaatcaaa ttgaaataac
    24601 gaaaaatctg agacagaatg acgttcattc tgacccagat ttgattttgc acaactcaac
    24661 agtccttatt ggcaaatcgc caaaataatg tagactaact aaaaagacta agaaacaata
    24721 acaaaagtta acacttctta gtctttcttt agcacgtcta tttattaaag attttagggc
    24781 cttttctcat gtttttacga tcattcttag gtgtttccat ttgtttggat cggcctccac
    24841 cttgtttctt tttgatcaat tctttcattt tttctttttg gtccattttt attctccttt
    24901 aacgaatgaa gcgactaatt cgatatgatg ggtttgcggg aaaagatcta caggctgaac
    24961 tttcttcacg gcataacctg cttctgcaaa taacttagca tctcttgcaa aagttgccgg
    25021 attacatgag atatagacga ttcgttctgg tgacatttcg attgctgatt caataaaatg
    25081 atgatctaat ccttttcgtg gagggtcaac tacgatcaca gatggtttga tccctttttc
    25141 ttgccatttt ttcataactt tttctgcttt tccgacttca taatgaacat tttcgatttt
    25201 attatttaaa gcattgaact gtgcatcttc aattgcttct ggaataactt ccatcccata
    25261 caccttatcc actctgtctg ctaaagacaa gccaatcgta cctattcctg aataagcgtc
    25321 gatcacaatg tcttttttat ctaactcggc aaactcgatg gctgtttggt acagatactc
    25381 tgcttgttga gtatttactt gataaaatga ctttgctgag attcgatagg tttttcctaa
    25441 tagctgatct tcgatatagt ctctgccata taaaactttt tctttgtctc ctaaaatcac
    25501 atttgttttt tcttcattga tattttggat gaccgaaacc acttctggca attcttcatg
    25561 aatagcttca gcaatttcct tggatttaaa gaatttttct ttacgagtaa ctaaaacgac
    25621 catcatttca tgggaataat gaccacgacg gataatgatg tgacgaagct gtccttcatt
    25681 tttttcttca ttatatcctc gtacttggaa ccgttgcaag atatctctga ctactttgat
    25741 tgcttgatcg atgacaggat cttggatata aaaatcttcg atagggacta gatcatggct
    25801 attctttttg taaaaaccgg tggctaactg tccatcgata cggcgaacag ggatctgtgc
    25861 tttattacgg tagccgaatg gttctgccat tccgaccgtt ggaagtacag gaacttctgg
    25921 catcttagca atcttcgcca ttacatgctc tacttgtttt tgtttgaaga caagctgttg
    25981 ttcatacttc aaatgggcta aaggtgcgat acctatgcga agtaatgcat tgttttctac
    26041 aggctgacga tcaggtgatt gtgtaataaa tgtttcgact tttccatatc caaacttatt
    26101 gcctgctttt actactagta tttctacttc ttctccaggg agtgcatttt cgataaataa
    26161 tggataacca tcgatttttg ctacccccat tccttcataa ctcaagtcga cgatttcgac
    26221 tgtatatcgt tcgtttttct ttactatttg ctgttctgtc atattttgct cctttaagcc
    26281 tatttttcaa tcctactgaa ctctactata aatcagctaa ttacacaact aaaaccggca
    26341 ctgaccttac tacgttcagc accggctttt gattaattct tttgattagg tgtttcgtct
    26401 gctatttttc cgtcaccgtc gatatcttca tccgttaaac gttcgacttc ttttacgaat
    26461 tctttgctga caacctctaa ttcttcttca tctgtacctg tgattgcttc atcaggaatc
    26521 tgatcggtat tggcaaagaa ctcaatgtgt tgatgaagat tttggaaatg catcggtgct
    26581 tccccgccgt attcaccatc taagttgatc attactggtc gattgatatc atcttctgtt
    26641 tcaacgaaca aactgcttgt tttggtatag attattcttg gatcatcgac atgtcgaccg
    26701 ccattcagca tgagtgctgc aatatgcaag atctcaaaga tatttgctgt cttcacaatg
    26761 attaaagaaa acttcccatc atccaatttg gcatctggcg cgatctgttc gaatcctcca
    26821 acagagttcg tcagccctag gaaaaacatc gaagcatttc cgatatattc cccttcatca
    26881 tacttcaagc gcattttgat tggctttact cgagggagca tctctgctcc tttcgcaaga
    26941 taagcaagat aaccaaaaat actctttagt tctgatggaa cttcataagt caattctgtc
    27001 aaatagccac ctgcagcgat attgatgaag taactgttat cagaaaggcc gatgtccatt
    27061 tttacggttt ggtgtttttt tatcacttct gctgctgctt taatattatc tctaggaatt
    27121 tttaatgctc gagcatagtc gttcgttgta ccagctggga tgatggccat tttaggacgg
    27181 tgttcaagcg gagcgattcc attaaccact tcgttgattg tgccatcgcc accagctgcc
    27241 actactagat caaaacctag ttctgcgact ctacatgctt cattcttggc tgaatctggt
    27301 tctggtgtag ttgcataggc gctggcttca aatcctgctt cttctaatgc ctgtaggata
    27361 tccgccaaat tttttttcaa taattctttt cccgaggtgg ggttataaat tacgcgtgct
    27421 tttttcattt tcagcctcta tcgtttcgct aattcatctt taagtagctt attcacgaca
    27481 ccaggatttg cttgtccttt agttgctttc atgatttgac cgactaggaa gccaaccgca
    27541 cgatcttttc catttttgaa atcatcgaca gattgttggt tgttatccaa aatttcatta
    27601 atgattggca gtagtttgtt cggatcagat aattgcaccc aaccattttt ttcaactact
    27661 tcttgggctt ctccgccatt tgtgatcaat tcacggaaga cttttttggc gatttttgag
    27721 ctgatcgttc catcggcgat caaacgaatc attcctgcta agttctcagg tgtcaatttt
    27781 gtctcagcta attccagttt ctcactgttc aagtaagcag aaacttcacc catcaaccag
    27841 ttagatgctt gcttggcatc tgcaccattt tccaaaacag cttcaaaaaa atcagacatt
    27901 tcttttgtaa gtgtcaatac cattgcgtca tactctggaa gtccaaactc acggatatag
    27961 cgttcgcgac gagaagctgg catctcaggc aaactagctt ttactttttg aatccattcg
    28021 tcatcgatca cgaatttcgg gatatcaggc tctgggaagt aacgataatc actagaccct
    28081 tctttgacac gcatcaaaac tgttttattc gttgtttcgt caaaacggcg tgtttcttgt
    28141 tggatttctc ctcctgaaag aagaactttt gcttgacgtt tttcttcaaa agctaaacct
    28201 tttttcacga aagataaaga gttcaagttt ttcaattctg ctttcgtacc gaactcttct
    28261 tgtccataag ggcgaagaga aatattggca tcacagcgca tagagccttc ttccattttc
    28321 acatcgctga cttctgtaaa ttggataatt gatcgcaatg cttctagata agcgtatgct
    28381 tcttctggtg aacgcatatc agcttcagaa acgatttcga tcaaaggcgt gccttgtcgg
    28441 ttcaaatcaa cgtaagaata accgccaata ccatgcatgt tttttccagc atcttcttct
    28501 aaatggacac gttcgatccg gatttttttc tttttacctt ctacttcgat ttcgatccat
    28561 ccgtcatgac cgattggttg atcaaattgg gaaatttgat atgcttttgg attatctggg
    28621 taaaagtaat ttttccggtc aaaatgagta tctttagaga tttcacagtt taatgccaaa
    28681 gctgctttca tcccgaattc cagcgcagct ttattcatta ctggcagcac gcctggataa
    28741 ccccagtcga tcacgtttgt attgctgttt ggttctgcac caaaatgagc tggcgctgga
    28801 gagaaaattt ttgagttggt ttttaactcg acgtggactt ctagtccaat gactgtttca
    28861 aaattcattc gtctttccct cctaaaatag ctggttgttg tttatggaat tctgttgctt
    28921 gttcaaacgc atatgccgct tgatacatcg ttttttcatc aaaataatta ccgatgatct
    28981 gcaatccaac tggtaatcct tctgataaac ctgccggtac agacatacct ggaagtccag
    29041 ccaagttgac agggatcgtt aagatgtcac tcatgtacat tgtgattgga tcgttaatgt
    29101 tctcaccgat tccgaatgca actgttggtg aagatggccc gatgatcaag tcataatctg
    29161 caaagacttt ttcaaaatct cgtttgatca atgtacggac ttgtcctgct tttttgaagt
    29221 aagcgtcata ataacccgcg cttaaggaga atgtacctaa catgatacgg cgttttactt
    29281 cttcaccgaa tccttctgaa cgtgagttga catatacatc atctaatgat ttcacatttt
    29341 cagagcgata accgtagcgg ataccatcga atcgttgcaa attggaactt gcttctgatg
    29401 aggcaatgat ataataaaca gccacaccgt attttgaatg aggcaagctg acttcttcca
    29461 caacagcacc taatgaacgg aatgtttctg ctgctttcaa tacagcttct tttacgccag
    29521 gggccacacc ttcgcctaag tattctttag gtaaagcaat cttcattcct ttgatgtcgc
    29581 ctgtcagtcc ctctgcaaaa tcaggaactg aaacgcctga agaagtaccg tctttttcgt
    29641 cataaccgct gatagctgtc aatgctaatg cattatcttt caccgtacgg gtcattggtc
    29701 cgatttggtc aagagatgaa gcaaaggcaa tcaaaccaaa acgtgagaca cggccataag
    29761 taggtttcat ccctacgatg ccgttaaaag ctgctggttg gcggatactt cccccagtat
    29821 cactacctaa agaaactgga atttgtccag ctgcaactgc tgctgcagaa ccaccagaag
    29881 agccaccagg tacttttgtt gggtcccaag cattttttgt tttcttgaaa taagaagttt
    29941 ctgtacttcc acccatagca aattcatcca tgtttagctt accaacaggg atcatatctg
    30001 cttgatagat tttatccatg accgttgcat cataaattgg ttcaaaattg taaaggatct
    30061 tagatgcagc ggttgtcaag atatcttttg ttacgatatt atccttgata ccaattggaa
    30121 taccagcaag gacgttgctt tcatcaatcc ctttttcatc taaagctttt gcctgttcca
    30181 atgctttttc ttcgttcaat gtaatgaatg cctcgacctc aggctctgtt tctttgattc
    30241 gttcaaatgt tgtttttgtc aaatcttgta cagtgatttc tttcgataca agcatatgat
    30301 gcagttcttc tagagaacga ttgtgtaatt ctgtcattat gcgccagcct ccccattatc
    30361 gatgattgcg ggtactttga taaagccgtc ttctgtttcc ggcacatttt tcaataattc
    30421 atcgcggttc catccagctt gcgcccgatc ttgtcgcatc acgtttactg tttcaaccac
    30481 attcgacgta aatggaacac cttccgtatc tacttcttcc agcatttcaa ccatatcgat
    30541 gattttacct aattggtcgg taaaacttgc aagttcttca tctgcgaacg caagttttgc
    30601 taatttggca acgtgtttca cttgttcttc actaatcgcc atactacccc ttctttctaa
    30661 acgagttaaa aactttgctc tctattttaa cacaagagtg ccgtgtctga aattgtttat
    30721 tgaaatttgt ctgaaaaaac tcatttttca ttcttctact gagcgacttt tagccaatgc
    30781 atctgccatt tgatcttctg tccaaacttc gattcctaat tcttgtgctt tggtcagctt
    30841 gctgccagca tcttctcctg ctaccacgat atccgttttt ttcgatacgc taccagtcac
    30901 tttcccgcca agattttcga tcgtctcttt tgcttcttca cgagtaaatc gtgttagttt
    30961 accagtaagg actactgttt tttctttaaa aggagactcg acttcttgca attgggcatt
    31021 tcgaagccct ttgtattcga aattgacgcc tgcttgtttt aattcattca tcaattcatg
    31081 gacttcttcg ttactgaaat aagtgactac actgtcggca atgatatcac cgatcgtatc
    31141 aagcgcaata atcgactcat agtcactttt actcaatgtt tctagatcac cgaaatgttc
    31201 tgctaaaatt tttgctgctt tcgctccgac atgtcggatt cctaacccga aaatcaaccg
    31261 ctcgaccgaa ttatctttgc tgttgtcgat tgctgtcaaa atattatttg ctgatttttc
    31321 ttttattttg tccaacgtca ataactcttc ttctgtcaat ttgtaaagat cagcaacatc
    31381 tgctactagt tttttatcat acatctgttc tagtacgcgc ggccccaacc catcgatatt
    31441 catcgcattt cttgaaacaa agtggttcaa accttctttc atttgagcag ggcatttagg
    31501 attgatgcac cgtaaggcta cttcttcatc caaatggaca agttcacttc cacaaactgg
    31561 acaatgtgta ggcaattggt attcttcgct gtctttaggt cgtttatcta acacgacttg
    31621 cgaaacttct gggatgatgt ctcccgcttt ataaatcaga actgtatctt tcaagcggat
    31681 atccttcaac ttaatataat caccgttatg caggctggca cggcttactg tcgttcctgc
    31741 tactcggact ggtgtcatga tagctgttgg cgttactacc cctgttcgac cgactgtcca
    31801 ttcgatgttt tcgattagcg tttcaacttc ttctggcgga aacttgtaag ctgctgccca
    31861 tctcggtgct ttgatagtaa aaccaagttg atcttgtagc gagaattcat tcactttgat
    31921 gacgattcca tcgatttcat agggcaaatc cacacgtttg tcatgatatt cttcaatgta
    31981 tgaccacact tcatcaatcg aatgacatag acgtttttcc cgattcgtgt ggaatccaat
    32041 tttttctagc tcttctagcg catcatactg ggtttttgct tgcataggac cgaaatcagc
    32101 tactgtgtat aaaaaagtgt ccagatttct tttcgctgtg attttagaat ctaactgacg
    32161 caaagaacca gctgcggcgt tccgaggatt cgcaaagata tcttttccct ctgcttctct
    32221 gtcttggttc aattggacaa atgaccgttt tggcatgaaa cattcgcctc ttacttcgat
    32281 gttcattggt tcttttaatt tgattgggac cgaccggaca gtttttaggt tttcagtgat
    32341 gttttctcct acagttccat ccccgcgagt cgcacctctc acgaaatttc catcttcata
    32401 acgcaaagaa acggaaagtc catctatttt tagttcacag caatagtcaa ctacacgtcc
    32461 cactgctttt tgtacacgtt gatcaaaagc tatcagctct tctttgctga aaacgtcgtt
    32521 caaactgtaa agcggaatat catgagtgac tttttcaaaa ccttctaata cttttccgcc
    32581 tactcgttga gtgggagaat cagacgtgat caaatcagga tattgttcct cgattgctac
    32641 taattctgcg tattctttat cataaacata gtcttccact gttggtttat cttctacgta
    32701 gtattcgcga gaccattggt tcagtcttgt acgaagttct tctgctcgtt tagcagcttc
    32761 gtcaatcgtc atttcatgtt ccatcattta ccctccacta ctaagctttt tcaattggcg
    32821 caaaggcagc caaaagacgt tttactcctt gacttgggaa agccacatct agttctagat
    32881 ccttcgacgt cccgcttact cggacgactg ttcctagacc ccattttttg tgtttgactt
    32941 tatctccagg tttccagctg ttattttccc ctccagtagc acttttagag ggaacaaccg
    33001 ctgtctctgg ctgtttatag ctatatgctg tttttttagc gaatacaggc gtttttttag
    33061 gcgtaggacg catgccttcg atttccagta actcctgatc gatttcttct acaaaacgag
    33121 aaggtcggtt atattgcgtc cgtccatata atgttctgga gaaagcattt gttaaataca
    33181 acgcttcttc tgctcgcgtg atccccacat atgcgagacg gcgttcttcc tccagttcgc
    33241 tttcttccat caatgctcga gataaaggaa aaaccccctc ttctagacca atcaggaata
    33301 caactggaaa ttctaatcct ttggctgcat gcaaagtcat caatgtcact tgggaagcat
    33361 cttcttctaa attgtcaatg tcggaaacaa gtgccagatc atttaagaag accgttagtt
    33421 tttcttctgg tgcatcagcg tcttcttcat tttgctgttc gaattgtttg tcgaattctt
    33481 gtgtgaccgt taaaaattcc tccaagtttt cgatgcgtgc ttgtgcttct aatgtatttt
    33541 ggatttttaa gtcttctaga tatccgctac gatccaaaac ctctttcgtc aattctgtca
    33601 ctgtcaagta tgggatcatc tgagtgactt cttggatcat ctctccaaat gcacctaatt
    33661 gctgtcctgc ttttccaccg atatttgcca gatccacatt ttgcgctgct tcaagcagtg
    33721 gccattcatg aagagaagca aaactgcgta gtttttcgat agatcctggt ccgatgcctc
    33781 gtttgggcga attaacgata cgctcaaaac tgattgaatc ttgaggattt accagcacat
    33841 tcaaatacgc taaaatatct ttgatttctt tacgatcgta gaacttgtgc cctccaacca
    33901 ttttataagg aatatttgct ttcaacagag tttcttccat tacacgagat tgtgcgttcg
    33961 tccggtacaa gatggcaaag tcgccgtaat tccgatgatt gcttcgaatc tcttcttgca
    34021 tccggtctac gataaaacgg gtttcgtctc gttcattatc tgcacggtag taagtgatct
    34081 tgtttccttc tttgttttct gtccacaagt tttttggttt tcgattgcta ttgttttcaa
    34141 tgacttggtt tgctgcgcta aggatgtttt tcgtggagcg atagttttgt tctagcaaaa
    34201 ttactgcagc atctggataa tctttttcaa aatccaagat gttctgcata tctgcaccgc
    34261 gccagccgta aatactttga tctgcatctc ccacgacgca caggttccta aatctacctg
    34321 ccaataaatt gactagcgta tactgtgcgt gatttgtatc ttggtactca tctacatgaa
    34381 tataatggaa tttgttttga taatacgtga gtgaatcagg atgttcttca aataatcgaa
    34441 tcgtattcat gatcaggtcg tcaaagtcca tacattgatt atattgaagc tctttttggt
    34501 aagcagcata acattttgcc gcaatctctt cgtaataact gccttgcatt tttgcaaaat
    34561 cctgaggggt ctgcaggctg ttttttgctt gagagattgt tcctaatata cttcgaggat
    34621 catatttttt cggatcaatg ttcagttctt ttagtatccg tttcatcaag gtcagctgtt
    34681 cagaagaatc gatgattgta aagttccggt tatacccgat aaaatcaaca tctcttctta
    34741 aaatacgcac acacatcgag tgaaaagtcg atacccagac atcttcgcct ccagatgcga
    34801 gaatcgcatt gacacgttct ttcatttctt ttgcagcctt attggtaaaa gtaatcgcta
    34861 ggatgttcca aggattgact tctttttcct caatcaaata ggcaatgtgg tgtgtcagca
    34921 ctctcgtctt tccacttcct gcacctgcca ttagcagtaa tggtccgtca gtgtgaagaa
    34981 cagcttcttt ctgacggggg ttcatcccgt ttaataattc agctttggat gacattaaca
    35041 acatcctttc ttttatcaat cttgttaatt atagcatttt cttggctttc attaaactag
    35101 ctagttcaaa atcatttgtt tctttttatg gatttttcct tgtcaattcg ttgatttcgc
    35161 gctaaattaa aagaaagcga ggcgtttgaa tggaaaaaaa aggaaaaatc aagaaattag
    35221 ataagactca gatatatttt attctaaaag gaatatttgt tggactgctg acaggaatcg
    35281 tcgttagcct gtttcgatta tctatcgaaa agctaagcga tttagttcga agtatctatg
    35341 aaatgagtcg ggaacagccg atttacttaa ttggcctagc tatttgctgc atccttgcag
    35401 catttttcgt gggttatctg gtcaaaaatg aaccggatat caagggaagc gggattccac
    35461 aagtagtggg acagcttcgc ggggaattat caatgaactg gttctctgtt ttatggaaaa
    35521 agttcattgg cggtgtactt tcagttggtg cgggcctttt tctaggtaga gaaggacctt
    35581 ctatacagct tggcgcatcc attggacaag gagctggaca attgtttcgg agtccatctt
    35641 cagaagaaaa gattttaatt tctagcggtg caagtagcgg acttgcagca gcattcaatg
    35701 ctcctatcgc aggattactc tttgttttag aagaaatcca tcactccttt tctccacttg
    35761 tttggctgac atcatttgct tcagcaatca ctgctaactt cgtctctctt tactttttcg
    35821 gcttgagacc tgttttatat ctaggtgacc ttccttcttt gccgttgaaa tattatggtt
    35881 ccttagtgat cttaggagta cttctaggca tcttaggttt tatttatcag aatgtcttgc
    35941 tggctttgcc aaaattttac aaccgattgc ctttaccagc ttattttcac gggttcgttc
    36001 ctttcttgct gattttgcca attggttatt tatggcctag tactctaggc ggaggcaatc
    36061 aaatcgtttt agcttttgga catgcttcct tgccaatcat tatgattatc ggtttgtttg
    36121 ttcttcgatt cgttttttcc atgatttctt acggtggggg tcttccaggg gggattttcc
    36181 ttccgatatt gacattgggg gctttaatcg gtagctttta tggaaacatt atggttgatt
    36241 tcgggatgga tccaatctac atcaaggatt ttgtcgttat tgccatggct ggttatttca
    36301 cagcgattgg aaaagcaccg ctgacagcaa ttattttagt gacagaaatg gttggctctc
    36361 ttaatcatct gatgccttta ggattggctg ctcttgttgc ttatatcgtc aatgaccttc
    36421 tcggcggtaa tccaatttac gaatcattat tggaacgtct acttggaaat actcagaccg
    36481 cctcactaaa aggtgcaagg acaacttttg attttccagt aacggcagag agttctttag
    36541 acggattaat ggtacgagac ttcaattggc ctaaaaatat gctgctaatc tctatacgca
    36601 gaggaaatca ggaaatcttg actcacggtg atacgatcat gaatgtagga gatattttgg
    36661 ttatcttgac tgatgaagca aacttgccaa gaatcaagca ggaaatcgag gctaaatctt
    36721 ctatcacaaa tttgaagcaa tcattttaac gaacatggaa aaactatttt acaacaacta
    36781 ttcatctaca ctacttgatt ctatatgatt ctataaaaaa agaagaggct gggaaaaccc
    36841 agcctcttct tttttatttc tatgcttctg ctactgcagg agcttttctc aataaaccat
    36901 aaatcacacc agatacgact gctccgatag caataaacac caaatacatg accgggtgac
    36961 tcagtaagaa tacaacgaaa atcccgccgt gaggagccag taatttgatt ccgaaagctc
    37021 cgactaatcc gccagttaaa gcagaaccaa taataaaact tggaatggca cgaatcgggt
    37081 cagctgctgc aaatggaatc gatccttccg tgacaaacga tagtcccatc acgatgttcg
    37141 tcaaaccagc atcttgatct gttttgctga atttattctt aaacaaacgt gtagcaacga
    37201 aaattgccaa tggaggtacc attcctccag ccataacaga agccatcacg acacttccgc
    37261 cagttgcaac agtcgtagct aaagttgctg taccaaacac ataagcggct ttgttgattg
    37321 gaccgcctaa atctgccgcc atcattccag ctaataatgc accaagtaaa gcagcattcg
    37381 aaccactcat acttgtcaag aaaccgttca atgcatcatt gattgctttc atagggacat
    37441 tgaccagcaa catcaagaaa ccagtaatca gtaagccaaa cactggataa aagaggatcg
    37501 ttttaattcc atccaatgat tttggtaagt tcttgaataa tttacgtaaa aatacgatga
    37561 cataaccagc taagaatcca ccaatcagtg cacctaagaa tcctgcaccg cctgtattag
    37621 ctaatgcacc agcagcaaat ccaacgatca aaccagggcg gtcaccgata cttgaagcaa
    37681 taaatcctgc taataccggc agcatgaaac caaatgcagc ttgtccgatt tggttgaacc
    37741 agctagcagc ttgattgtaa ttccctagat ttgataattg atcatgtggc acgccaataa
    37801 attggtcgat catgaaagaa agcgcaatag cgattcctcc accaataacg aaaggtaaca
    37861 tgtgtgatac gccgttcatc aaatctttgt aaattctttg tccgatagta ccatccgcat
    37921 ttccttcttc tgaactgctt tcttttccat caccatggaa tactggtgca gtaccactaa
    37981 gggctagatt gatcaactct tctggtttac ggataccatc actgacagga cgattgatca
    38041 gttcttttcc gtcaaagcga ttcatctcta cttttttgtc tgctgcaacg attacacctt
    38101 ctgcacgggc gatgtcttca gcagtcaatc ggtttttgat tccttctgaa ccgttcgttt
    38161 cgactttgat ctccacaccc atttctttcg cttttttctt caaagcatct tctgccatgt
    38221 aagtgtgagc gatccctgtt ggacaagctg taacggcaac aacgaatttt ctttctggat
    38281 gagcagaagc ttctgcagct tgtgtttctt gttctgcttt ttcttctgct tctttttgct
    38341 cttcagctgt ttggaacaag tcttggactt cttctggtgt atttgcttct tttaattttc
    38401 ctacgaaatc tgcatcaatc agcaatcgag atagtgctgc taatgcttgc aaatgtgtgt
    38461 cgtttgcgcc ttctggagct gcgatcatga aaaacagata agttggctgt ccgtctaagg
    38521 cttcataatc tactcctttt tggctttttg cgaataaaac tgttgcttct ttaactgcac
    38581 tgtttttcgc gtgaggcatg gcgattccat caccaagacc tgtagaagtt tgtgcttcgc
    38641 gggctagaat tccttcttta tatgtttcga tatcggaaat ccggccggca tcaaacattt
    38701 tttggaccat ttcatcgatt gcgccttttt tatcagtcgc ttgcaaatcc atgatcatcg
    38761 cttctttgac cattaaatcc ttgatattca tgattatcct ccttgaaact aatcaatttt
    38821 tgtgatttca acttctggca gcaactcatc aatcaattcg cgagctgcca agtcatcaga
    38881 aaaagctgtt gcgcttccgc aagcaactcc ccatttaaag gcttcgattg gatctttcgt
    38941 acggctgtaa gccccaataa agccagcaat cattgaatcc cctgcaccta ctgaattttt
    39001 caatgggcga actaatacat tcgttttata agttccttgt tccgtgaaga gcaaagcacc
    39061 gtctccagcc attgagatca gcacattctg tgccccttca gctagtaatt ttttcccata
    39121 cggaatgatt tcttctacag attggaaggt tgtgttgtaa agctcagcta attcgtgatt
    39181 gtttggtttg atcaacaacg gtttcttaac aagtgcattt tttaggtcat cacctgttgt
    39241 atcaatcacg aaatctgcac ctttttctcg aatgatatca atcagttctt gataaaatcc
    39301 ttttcgcagg ctagctggcg cgcttcctga caatacgacg atatctcctt gtcctactgt
    39361 tcctaatgct tgcttcaact catcgatttc ttcatctgaa atagatgggc ctaagccatt
    39421 gatctcagtt tcagtgtctg attttagttt gatattgatt cttgtatctt cttttacggt
    39481 tgtgaacaaa gactggattt gttctttttc aagccacttc gaaataaatt cgccagtgaa
    39541 ccctcctaaa aaccctagtg ctgtagattg agaatcgatc cgtttcaaaa tacgggaaac
    39601 attgatccct tttcctccag gtaacttgaa gtcttccttc attcggttca aatcgcctag
    39661 ttttaaccca tctacgcgta cgatataatc aattgatgga ttaagtgtta ctgtgtaaat
    39721 cattgtattg cctccttaat tgttgtcttt tcacggaatt cattaacgaa ttctaatgga
    39781 caacgctcgg ttatgatcgt ggctgcttcc aaaggtgcaa cagaggtgaa ggacaactgg
    39841 ttgaatttgg tatgatcaat caatacaaat gtctcttcgg aatttttcat ggctaaacgt
    39901 ttaacagctg cctcttctgg atccggagtc gtgaatcctt gttcaagatg ggcaccgttc
    39961 attcccataa atgctttatt aaaatgaaaa ttttttagtt gttctatcgc attagatcct
    40021 aaaacagcat tagttgccag ctttatcgtt ccacctaaaa taatggtagc aagctccaaa
    40081 tcaactaaat ttgccgcatg ttgaacggaa tttgtcacta ctgtgatctg ttttccttgc
    40141 agaaaaggaa tcatttctaa cgtagtagag ccagcgtcta aataaatgac atcttcgtca
    40201 ttaatggttt cagctgccga tttggcgatt tgctgttttt cgtgaacgtt tttgagtgat
    40261 ttttcactca tattctgctc aaaattcaat aattgctctt tcttagctcc gccatgaact
    40321 ctttctagaa acccactatt ctccagctct tgtaaatcac gtcttatcgt tgattcagaa
    40381 gcattcaaaa ggtttacaag atcttgtgat ttcacgattt tctgctgttc aagcaagtct
    40441 aggatttttt gtctgcgttc ctctgtaagc attttcacga tcacctcaat acaaataata
    40501 gcatattttt tttctactag caatataaat cagtcaaaaa cgttcataaa aattataaat
    40561 cagtcaaaaa aaaataaaaa accagtcaga aagactggtt ttttattttt ttgtctatca
    40621 tcaagccgtt ttatcgtttt ttattttcgc ttcttgtttt tttcatataa acagaagcag
    40681 gttttctttc cttatgaaga agataataat aaagtcccat cccgccaaac atgaccaaaa
    40741 aaccgatgac tgatcgaata ggaaaattag gaacaaaaaa gagacagacg gataataatg
    40801 atgccggaac cattattcga taatagagtt gtttatccat tttcttcacc tcatattttt
    40861 tgtaaagtaa tcaattgttc taattctcgg taatctaaaa attgcacacg cagatgctgc
    40921 tcacctttct tgacatcgaa ttggatactt cgccgctttt tctttattcg tttgaaggta
    40981 acatcagaga gtctatgttt gatttccttt aacggtaatt ctgacaggga ttctgaagga
    41041 acatagattc ttaaggataa aagatactct tgatattttt catctgttac ctctacttct
    41101 agatattccc taatataata acaataagct tcgattccat tatccaaagc aacagtcgga
    41161 tcgataactt tatagcacat catgcgtgat cgattctcgt caacccaagg aaaatacgcc
    41221 tgagcattta gtgttccgcg aacacgcctt tctttataat ctccttgttg gatatcataa
    41281 ctttttgtta caaaaaatga agcattcttt ttaggaacag gttgcatttg tgcattctga
    41341 tactggacag attggatcgt atggtcagtg taaatgtatt caacgaatgg cggtttcaat
    41401 aaaccgacac cattgtccca agcaagatga tcaaacccaa gctggtccct aaaataggat
    41461 tgaaacattt gattgtttgg cttttgccag ttaaaggggt ctcgataaag ctggatacat
    41521 atgacttcgt tttctaattc cttcactttt tctacttgtt ggatgtcgag aaaaatcggt
    41581 ttagggatga tttgatgata atagcgacta taatacattt cccaacacga agtgaaacat
    41641 aaaccttggt aaaaaagatc gtatcctgga aattggttgc aatcgactac tacttccccg
    41701 ttttgttgtt tcattttagg taaatgttcg atttcatcct ttttctcaaa caatagccgt
    41761 tgagagatct gttttgtatt gtgatagata aactcgctat aagctcgcat ataggcgaac
    41821 accccatgag caatcatttt tttctgaatg aagtctcgaa tcaaaaattg cttttctttg
    41881 aaaacagaga tagacaatac attttttatc aataattgat tatcttccaa gcgaaaacta
    41941 cattcgttct cgtcatttcc tacaactaac caactttcgt ctcttaccga tgctagcatt
    42001 attttttctt gaagctttcg aagatcatac ggatccgctt gttgtgtgaa agagcggata
    42061 aatgttggct ggatcaacag acgtgtcaaa atagcccaat cgcttatttc ctgttttttt
    42121 tcttcttcat ttaagtctgt gagctggata gtcatcaata atttatcttc gccaatcatt
    42181 ttctcacctc tttcaaatct ttttataatt taattataac caatgataaa gaaaaactaa
    42241 aactttatgt cttagaaacc aatttggacg attacatgat agactaagta aaaagaaaca
    42301 aagaggtgtt tacaatgggg ttgaaatttg aacgtacaga taatttagat aaacttttcg
    42361 aatcatttgc tgtcgatccg gaaaaaacag ttgcaccaaa agaagagatc gatcgatttc
    42421 taaaaccaca gccacaaaat gaaaagaaaa aagacaagaa acaaaagtaa gttaagctca
    42481 aaaaaagact taccagccaa acttttatga aagagtttga ctggtaagtc ttttttattc
    42541 tttttatgat tcaattgctt gttcgattgc tgcccacgct tcttcttttc cctgttttgt
    42601 aacggaagaa aagagaataa acgtgtcatt gcgatcaaaa tccaacgctt ttttgatttc
    42661 gctctcatgc ttgttccatt taccacgagg gatcttatct gccttagtgg caacaacaat
    42721 tacagggata tcataatact tcaaaaattg atacatctgg atatcttctt gagaaggctt
    42781 atgacgcaaa tctactaaag aaacgaccgc acgtagctgg tctctagtcg ttaaatatgt
    42841 ctcgatcatc tttccccatt ttgctcgctc agttttggat acttttgcat acccatatcc
    42901 cggaacatcg acaaaatgta atgcgttctc gatcaaataa aaattcaatg tctgggtctt
    42961 tcctggctta ccggaagtac gggctaaatt ctttcgatca atcaatgtgt tgataaaaga
    43021 agatttccct acatttgatc gtcctgccag agctatttct ggcaattgtg tatctggata
    43081 ttgttttggt gccactgcac ttatgataat ttcagcttga tggactttca tggtctctct
    43141 ccttaacaag gtgatgaata aagcaagcgg ctgtcgcaaa agccaaaatg acttttacgg
    43201 cagcctcttt tcatcaaccc gcttttttct ctgaatcttt atagattact gttggctcac
    43261 cagacaattc agctgcttct tttgtgataa tcactttttc gatgttttca tttgaaggga
    43321 tatcaaacat cacatccatc atgatttctt caatgatgga acgaagtcct cgagcaccgg
    43381 tattgcgttc gattgctttt ttcgcaatgg ctctcaacgc ttcaggttcg aattcaagtt
    43441 cggtattatc tagagaaaga agtttttgat attgtttgac tagcgcattt ttcggctcag
    43501 tcaagatccg taccagatca tctgttgtca atttctctaa agctgccata acaggtaatc
    43561 gtccgataaa ctcagggatc aatccgaatt ttagtaaatc ttcaggaatg atatgctgca
    43621 tgacactttc gtcttcagaa agtttttggt tttgtgtacc aaatccaatc gtcttttcgc
    43681 ccatacgatt cttcacaatc gtttcgatgc catcaaatgc accacctacg atgaacaata
    43741 tgttcgtcgt atcaatctga ataaattctt gatgtggatg tttacgtccg ccctgaggtg
    43801 gcacacttgc aacagttcct tctagaattt tcagcaaggc ttgttggaca ccttcgccgg
    43861 aaacgtcacg agtaatagat acattttcgc ttttacgcgc aattttatcg atctcatcaa
    43921 tatagatgat ccctttttct gcaagttcca cattgtaatc agcagattga agcaatttaa
    43981 gcaaaatgtt ttctacatct tcccccacat aaccagcttc tgtcaaactt gtcgcatctg
    44041 cgattgcaaa aggtacattc aatgtttttg ctaatgtttg tgctaaaaat gttttaccag
    44101 accctgtagg tccgatcaag cagatattgc ttttttgcag ttcaacatca tctgtgtttt
    44161 cttgtgcttg ctgattgaca cgtttatagt gattgtatac ggcaacagct aacgagcgtt
    44221 ttgcgcgatc ttgtccaatc acgtagttat tcaatacttc aagaatttct agtggtttag
    44281 ggacttcagt gaattcgcgt acagcttctt cataaaattc ttcatcaatg atttctttac
    44341 ataggtcgat acattcatta cagatgtaga cccctggacc agctacgatc tttttcactt
    44401 cttcttgggt tttcccgcaa aaagaacagc gtacggtctc attgctactt ggattgtcat
    44461 acatggtctt caccccttaa cttgcaaaaa gtatgcaaga aactatcaaa gactttacag
    44521 tcttttacta tcataacata gtttagtaaa aagaaaaagt gtttaacaaa ggatcaactg
    44581 acctatacct ttgcattttt tactcttaac aaaaaataag gaactgtgac aaaatcgtgt
    44641 cacagtccct tatttcgaat aaacaatttt ctagaagtag cttcttcaga aatgatttat
    44701 tacttgattt ctattctttc gcttctgtcg ctgtacctgt gatcaattcg attgcttttt
    44761 tcattgtcac atcatgttcc agcatatctt ctgtcaacac gcgtttgatt tgttcgattg
    44821 gcatgttgta tgtttctgat aattcttgga tttctgcatt gatttcttct tctgttgctt
    44881 gaagattttc agcagctgca attgcttcga tcacaagatt tgttcttgtg cgcatttctg
    44941 cttcaccttc gaattgttta tgcaagtctt cttctgtaga accagttaat tgatagtaca
    45001 tttcaggaga gataccttga cgttgcatgt tgttcaagaa ttcgtccatt gaacgatgta
    45061 cttcatcatg aaccatcacg tgtggcaatt caacgatttc tgcgttttca acagccatac
    45121 ggatcgctgc ttcatctttt gcatcgtccg ctgctttttc ttttgtttct actaattctt
    45181 tgcgatattt ttctttcaat tcgtctaatg ttgatacttc gtcatcaaca tcttttgcga
    45241 attcgtcatc taattcaggc acttcttttg cttttacttc atggattgtc actttgaaga
    45301 ctgcttcttt tccagcaaga tcttctgctt gataatcttc tgggaaagtt acatttacat
    45361 ctagactttc acctgattta tgaccaacta attgatcttc aaaacctgga atgaatgagc
    45421 ctgaacctaa ttcaagtgag taattttcgc cttttccgcc atcaaacgct tcgccatcta
    45481 caaagccttc gaagtcgatt actactgtat cgccattttc agctgcagca tcttctttga
    45541 taactaattc agcttgtgct tcttgttcac gtttgatacg tgcatccacg tcagcatctg
    45601 ttacttcacg gtcttgtttt tcaacaacta gatctttata ttgtcctaat tttacttctg
    45661 gtttcactgt tacttcagca gtgattaccc agtcttgacc tttttccatg ctttcaacat
    45721 cgatttttgg ttgagaaaca ggatcgatgc cagcttcttt gacagcagct tcataagctt
    45781 ctggtaatac agcattcaat gcatcttcat ataaagcttc ttcaccgtac atacggttga
    45841 atacttgacg agaaactttc cctttacgga atccaggtac gttcaagctt tttttcactt
    45901 tattaaacgc agttgttaat cctttttgga tttcgtcttg cgcaatcgta aattttaaaa
    45961 caccatcatt cgtgcctttt ttttcaaatg ttgcagtcat ttaattccct ccgagtttag
    46021 aaattcattt tatacatcaa aaacgtttca tgcatacctt taaatagtac actaaccctt
    46081 tcaaattgta aacatattga atggatagaa tccttgtttt tcataacatt tttatctttt
    46141 tcactgataa aatctaatgg aagaagatca tttgctgcaa aattttttct ctttttttta
    46201 aaaaatcttg tttttcatct tctgtacctt catacatcgt ttttcctgta cacgcctcta
    46261 cagtcagttg aaaccagcgc ttcgggtctt cgatgtatgt atcatagaca ggataaacaa
    46321 ttgcagattg caacgtgaat tcttctttta acacggaact aagcagcgca tcttcattcc
    46381 cgtaacgatc tgaaaataca cataattgct ggtaacttgc ctgctgctgc ggctttggca
    46441 gatcttttgg tatgactttg actaactttt cttcaatcgt caaatagaaa acttgatggc
    46501 attcgttcaa ttgcctaagt gtctccagta attttgccct tgctaaaata tggacctctg
    46561 gcgatacaat aagtttactc gctgtttgga taaactcatg ataaggaagc tgttcgatct
    46621 catcaataga cgcaagctga cgtgcaggtt ccaacgaagg aagttcctcc gatttattgt
    46681 ggagtgcctt gatcttctgc ctctccattt gtccggataa ttcttctgca aattggattt
    46741 tttcaatgac ccgctgctcg aaagcttcat aaaaatctgt acttgctatc aattttctag
    46801 catataaaaa atcttgtgta ctgattaaca agtcaaaata aaattcagca aactcctcat
    46861 tttgaagata ctgtttttca tgcaagagtg cttgcttcaa tgcttctttt ttctcgtcca
    46921 actcgtaaag acaaaaaaca agcaggctat tagcctcaaa gctttctttc aactcgtaag
    46981 ctcgttgaaa taattctttt gcctgctgat attgctggtt tgctagtgct tgttgtgcca
    47041 ctcgcattaa attctcatat ttttctggaa aatcaatcgg cttcacgcag tgtcctcctc
    47101 ttgctcatat tcagctcatg ttcaatagta gcaaaacaaa aaaagcatga caattaaaaa
    47161 taactgaagc accctctaaa agctagattt tagatctgac ttttagaagg tgctttcaac
    47221 tgttactgct ttttattgct catcagctgt tgacttcctg gttgaacgct ttgatcagat
    47281 tgatcgtaac aacacttgta ttagtgaaaa aatcgcccag atgttgcttt ttctgattaa
    47341 agactgctgg aagataccct aacatcagca aaggaaattt tagaatgaac cggcaagcac
    47401 cttctctaac taaaacggtt gaccagctaa gttcttcttc atcaaagctg atgactcgaa
    47461 taccaaagat cattttccca atcgtttggc catgattgaa tttcgtcaat aaaataaaat
    47521 acgctagata aataagtaag gctgtgaatc cgtagagact cagataagaa tctgaaaaat
    47581 taagccaacc tagatttttg gcaataccaa gtgttaaacg cgtaatagct ccgatacaca
    47641 aaagatcgac caagaaggca aacattcgga taaaaaaacc tgcatagaaa tacttaggaa
    47701 aatcatttgc ttttggcggt tcatttcttg tgtccgtata ctttttccat ttacgttgtt
    47761 tctttttgat ttcttcgggc gtcaaaggct ctctttcaaa agagctcagt accttttggg
    47821 aaaagttgga gtcaggtcct acagcaggct gctgttgctt atctttttct tgttccttat
    47881 tttcatttga aagctctgac atgcttactc acctccgtaa taatacattg cttttggtga
    47941 ttccgctgtc cctagattct caagaacgga taagatctga ctagtctcag aaggcttcaa
    48001 tccctggaat tctgctagtt tacttcctaa ccatgtacta gcaaacccag atggcgtgga
    48061 agaatattct actagttccg catcttcaag ctgttgttcc tttcgtatag cttttaaagc
    48121 atcttcagga aatcccaatt catcgactaa acctacttct tttgcttgga caccatcata
    48181 aatccttcca tcagcaattt ttttgacttc ttcttcagat ttattccgtc cctcgctgac
    48241 gattgctaca aaacgactat atgcgctgtc gatatatgct tgaagcaccg cttcttcttc
    48301 ttttgtctct ggtcgcgttc ctgaacccat atctttcaac gctccgcttt taactgttgt
    48361 atcttccaca cccagttttt ctagaagtcc tgaataattc aatcctgaca tgatcacacc
    48421 aattgagccg gtcactgttt cttctgttgc aaagatttta tctgcttgag cagaaatata
    48481 gtagccaccg ctagctgcca tgtttttcat gctgacatac attgggatat catgttcttt
    48541 acgaatcgta tccaatttct tagcgatttc cgcactttcg tagacaccgc caccaggaga
    48601 atttacttct aaaaagatac ctttgactgt tggatcttct tcaatcgcct tcagttgttc
    48661 cataaaattt ttatgattgt aaccttcaga agcaaataaa cctgaagacc ctcccgcagc
    48721 aatcgttcca tctactgata attttacgat tttttgcgta ctgtctccct cttccaacac
    48781 atttggagct agttcttcat ccccatataa ccaactgttc aatccctcaa tttgatcgtt
    48841 ttcctgaggc ttagtcaaac tagctgaaac taacgaaacc acaaataatc ctgcagcaat
    48901 cagaactgct gcccaacgtc ttttattcat tctttctcct ttctaataca agttttttat
    48961 gacgatcaca gtttctgact gctcagaagt gtcgaaccgc tctgcttttt cttcccgcgc
    49021 atacccggaa aacgactctt ttgttaattc atcttctacc gtaaactcac gagcttgagc
    49081 aacttccaag gcatcaggtg cgatttcttg gatgctatcc cagccagtat ctaaaaatat
    49141 tttcgtatca tctgatacac cagctgatgc ttcgaatttg gacaattgtt tttttaacat
    49201 tcccagtgtc atgatctcaa tttttgaatc catttttact cctcctctat ccgattactt
    49261 tctattgtac cgaactcctg aaaaagatga tagataaaaa cccattattt tttgacataa
    49321 aaaggacatt tgacaaagaa tttgatatat accaatttat tttttattct ttttttgttg
    49381 acaagaacat tcgcaaaagg ttatgatttt aaatgtttcc agataaaaaa caaaaaggag
    49441 gatagttata ccttttagga tagcgccagg tctgtgtaaa cagatgacga ggagagggtt
    49501 tatcgaaaga ttcggcggat ggccctaggg actgcactct acagaaaatc cacaaaaaat
    49561 gtctgcaaag ataaaacaaa aggattttcc tgcagctgga agcattcata cgaattcatt
    49621 atagaaagta ggatgttttt tatgtgcgga attgttggaa tgattggatt aaaaaatgta
    49681 acaccaggat tgattgacgg tctagaaaaa ttagaatacc gtggttatga ttcagctggt
    49741 atctttgtct cagatggcac aactgattat ctggtcaaag cacaaggcag aattcaaaat
    49801 ttgaaaaaca aaataaatat tgatactaca ggaaatatag gtattggtca tacacgatgg
    49861 gcaactcacg ggcaaccttc tgaagaaaac gcccatccac atacttccca aagtggacgc
    49921 ttcgtgctcg ttcacaacgg cgtaatcgaa aactttgaag aacttaaaat tgcttattta
    49981 gcaaatgacc actttattgg tgagaccgat actgagatta tcgctcatct aattgaaact
    50041 ttcgcgaagg atactacaac gcaagaggcg tttttaaaag ctttgcgggt catcaaaggt
    50101 tcttacgctt tcgctttgat tgatcggaca gcacctgatg ttatttacgt cgctaaaaat
    50161 aaaagtccac tattaatcgg actcggcgat ggattcaatg tgattgccag cgatgcaatg
    50221 gctatgcttg ctcatacgaa ggaattcgtt gagattgaag atgaagagat ggttaccgtc
    50281 acatcagaaa aagttattat tcaaaatttt gcaggtgaca tcgtagaacg ttcttcattc
    50341 gaagcacaag tagatgcttc agatatcgag aaaggtacct atccttttta tatgctgaaa
    50401 gaaatcgacg aacagcctat tatcatgcgt cgtatcgctc aaaaatacgt aacagaagac
    50461 aatcgtgtct ctcttgatca aaagctggtc gatacattgt ctgatagcga tcgaatctat
    50521 atcgttgctt gcggaactag ttaccatgcg gggttagcag gtaaacagac tttggaaaaa
    50581 ttgacacaga ttcctgttga ggttcattta gccagtgaat ttggctataa cacacctctt
    50641 ctttcaaaaa aaccattctt tattttcttg agtcaaagtg gcgaaacagc agacagccgc
    50701 caagtgctgg taaaaatcaa ccgtctcggc tacccttcac ttacgattac aaatgttgct
    50761 ggttctactc tttcgcggga agcatccttt acactgctat tgcatgctgg acccgaaatc
    50821 gcagtagcat caacgaaagc ttatactgca caaatagctg tactaacttt attagccaaa
    50881 gctatcggtg ataaaaaaga atttgaaact tctcttgtat tcgatatcac tcatgaattg
    50941 agtttagttg ctaatgcgat ggaatcggtc atcgcccaaa aagattattt agaagagttg
    51001 gcagaagtat atctcagcga tacaagaaat gccttttaca ttggacgtgg tgctgactac
    51061 aacgtttctc ttgaagctgc tttgaaattg aaagaaatca gttatatcca ggcagaaggt
    51121 tttgccgctg gcgaattgaa acatggaacg attgctttga tcgaagaagg aacaccagtg
    51181 atcggaatca tcagtgaaga agtgactggt gcgcatactc gaggaaacct gaaagaagtc
    51241 gaaagccgtg gcgctaaaac actagttatt gtctcagagg gattagaaaa ggaaagtgac
    51301 cagctggtgc taccaactgt ccacccttat ctaactactc taaccacagt cgtaccgact
    51361 caattacttg catattacgc aacgctatta cggggattcg acgttgacaa accacgtaat
    51421 ttagcgaaat cagtgacagt agaataataa aagaacaagg accagtgtca gacatctgac
    51481 actggtcctt gttcttttat tttttatgaa aatatctctt aatccagtcc gatttcttct
    51541 ttgactactg ccgcgatttt atcgacatag taattaactt tctcatcaga aggcgcttct
    51601 gccatcactc gaagtaatgg ttctgtaccg gaaggacgaa ctaggattct tccttctgat
    51661 cccatttcag cttctgcctc ttcaatcact tttttgattg ccggaacatc catcgcaccg
    51721 tttttatcac ttacgcggat attcactaat ttttgcggat agattgttac ctctgctgca
    51781 agctctgata atttcttacc tgtctgtttc atgatattca gtaactgaat tcctgaaagc
    51841 atcccatctc cagttgtgtt aaaatctaag aaaatcatgt gcccagactg ctcgccgcca
    51901 aagttataat cgtttttgcg catttcttct acaacgtaac gatcaccaac ttgtgtaacg
    51961 acatctttca aaccgattcc ttccaccgct ttgtggaatc ctaagttact catgacagtc
    52021 gtaacgatcg tatctttttt caatcgattt ttttctgcca agtatttagc acagataaac
    52081 atgattttat caccgtcgac aatgtttcca agttcatcca ccgcaataat acgatcgcca
    52141 tcgccatcga aagctagtcc agcatctgct cctttttcaa cgaccatctc agctaaacgt
    52201 tctggatggg tagagcctac gccgtcattg atatttaacc cgtctggatt tgtgcccatt
    52261 gtataaaaat ctgtttcaag atcggcaaac aaacgattaa cacttgttga agttgctccg
    52321 tttgctgcat cgacacaaat ggtcaaaccg gaaaggtcac cgttgatcgt ttgtacaagg
    52381 aattgagaat atttcaataa gccttctgga aattcttcta cagttcccaa tccttcagct
    52441 gatggacgag gcaattcatc cacttcagca tctaataatg cttcaatttc tagctcttgg
    52501 tcatctacta gtttaaaacc atctgcaccg aaaaatttga ttccattatc ttgtgctgga
    52561 ttatgagaag cagaaatcat gacccctgcg cttgcttttt gcaaacgtgt cagataagca
    52621 acacctggtg tagagatcac acctagctgg aacacttcga tacccaccga taaaagtcct
    52681 gaaatcagcg cttcttctaa catttgacct gagattcttg tatcccgccc tactaacacg
    52741 cgtgggcgtt ttccttcttc ttcatgctgg cttaggacat aacctccgca ccgtcccagc
    52801 ttaaatgcaa gttctggcgt aagttccttg ttggcttcac cgcgtacgcc gtctgttcca
    52861 aaatatttac ccatagttgt aaaattactc ctttaactta attttctgag gaatttgttc
    52921 tgctcatttg tgttgattcg cttgtttctg atgtttcttc tgtacttgtg gtcgagctgc
    52981 ttgtcccaga ttccgaactg gaagaaatgg ttgttgtcga tggttccgtt cctgaatcag
    53041 cagatgaaat acttctactc gtacttgtat tttcttgtgt ccgactgctc gtcgaagcag
    53101 agctactgct tgacgtttgc aacacgggtg taatctccac actaactgat tcgggcttga
    53161 tacttaaccc ctcttctact ggtatttcaa ttttacgagt tgttcgatga ttgatacctg
    53221 agacattgat cggtacagca atactgtcac cgatcgatgc aagcaaatcg ctagagccgc
    53281 ttacttctgc ttccagtttt gaaaaactga aatcatagct ttctaccccg gcagctggtg
    53341 ttccttgctg tatcccataa agtcgtaccg acttgcttgg cttgatcgaa tcagctctca
    53401 ctgttacttg cactggatca gagacgatac ttaatacttc ccctgctgca ttcagcgcct
    53461 gtattgggac ctcactatta atcccatcat ctgtgaccat cgaagcgtcc acatcagcta
    53521 ctactcgttt gatttctgcc aatgttttgt cacctgtagt gatagagact tttttaggac
    53581 taacggaagt atcatcaagt tcatatccag atggtgtgaa cgttgaagga atcaacgttt
    53641 ccacatcgaa atcttttatt acctttttct caaccgttac tgtaatagtc gatggttcga
    53701 tctttgctgt gacagcactg ctcaaatttt gaatttttag cggaacttca tgtgttcctt
    53761 cgcccagctt agtgatatct gctgtgacgc ggaacatccg tgtatcttcg tttgcctctg
    53821 cattcaactg aactcggttt gcactggaaa gtttgacagt cactgcattt tcatatccat
    53881 ggatatagta gcgctttgaa tcataagaaa tcttgatcgg cacccccgta actgtttgat
    53941 tatacgcttc atttccagaa agcgtatttg gaacggactg gccatttgca ttgaagtaca
    54001 gcgcaatact gaacagcaat gcaataaaac tgtaaagaat gcttgtccgt ttctcttttg
    54061 agatcatttg cgatcccctc ccttagtcat accgtctagg aaagattgaa tcacgttttt
    54121 cttgtttttt gttttctctt cagcaattag ttctctacgc aagattgcca aatattcttg
    54181 ctgggataag ccagccatca gttcattatc taacgtgatg ctgacgtccc cggtttcttc
    54241 agagacaacg atggtaatcg cgtcactgac ttcgcttata ccgacagctg cccggtgtcg
    54301 agtcccgaat tctttaggga tcaacatact ttctgataga ggaagatagg cgcttgctac
    54361 tgcgatcttt ccttctttta caataactgc accgtcatgc agcggtgtat taggaataaa
    54421 gatattgatc agcagctccc cagtgatatc tgcatctaaa gcaatccctg tctcaatata
    54481 ctcatctaat ccagtatgac gttcgatagt gatcaacgca ccgatttttc gcttggacat
    54541 atattggatg gctttgtcaa aagacaagat catcttctca tcttcctgct gttcgctttt
    54601 tgattgtttg aaaagcgaac tgcgtccaag atgttccagt cctctccgga tttccggctg
    54661 aaaaatcacg atagcagcta tcaccccgta cgtaatgact tggttcatca gccaagacaa
    54721 ggtgtgcaga ccaatgattt ccgctacgat tcggataaca ataaaaatag ctacaccttt
    54781 cagaagctga atcgctcttg tgcctcgcag cagttggatc agtttgtata caagatacca
    54841 gacaaccaga atatcaagta tgttcaccaa aaaattcaga gagaacaagt caccggaaaa
    54901 catttgctgc caatagtcca aattaaaaag ttccccgatt tgaaatgaca taaaaagcct
    54961 ctcttctctt ttgagatttc ctctctagta tatcatatgt tttaatttta gaggctgata
    55021 tgctcgtatt tttcttgaga aaaacgcata attttcaaga aaattgcaag tatgcgtttt
    55081 atttttctct gcaataaata tttttaaact tcagagaatt attcattata ttttttctgt
    55141 tatttttcct ttttttcttt tgtagcttgt tccatttttt tacgtcgttc tgctctggca
    55201 gctcttctag cagctcttct ttcttctgct tcaaggtccg tcgttttttc ttttctagtc
    55261 ttaacgggta catcgataga ttcctctggt tcaagtcgtt tcttgatttt ttctaattct
    55321 cccgtcagtg cagcatagtt gtaaaaacgt gttttagcgt cgtggacagt cttttcaaat
    55381 aaagcttttg caagatttgg atttgcgtta tctagcgagg aaaagcgaac ttgtttcctc
    55441 atgaaatcag gcatagcaga gaaatcaggt tttttgtagt ccaaagtcat tgggttcttg
    55501 tttttttcga ccattgtagg gttatatcga tataatgacc aataaccaga agaaacagct
    55561 tcttttgctt ctttaagcga ttctttcatt ccgccaagca agccatgtgt gatacaaggc
    55621 gtataggcaa taatcagaga aggaccagga tacttttctg cttcttcaaa tgccttgatc
    55681 gtttgcattt gattggcacc tgaagcaatt tgcgccacat aaacatttcc atatgtcatc
    55741 gccatcattc ctagatcttt ctttgaagca tactttccac tagccgaaaa tttagcgatt
    55801 gccgaagctg gtgttgcttt tgaggtttga ccgccagtat tggaatagac ttcgttatcc
    55861 aaaacaagga tattcacgtc tgctccgctg gcaagtacat gatcgatccc gccaaaacca
    55921 atgtcgtaag cccatccgtc tccaccaatc atccattggc tgttttttac gaatagatcc
    55981 tttctcttgt atatatcctc cagttcaggg aaaattgatc gttcgctttc gatcgcagct
    56041 tgcagttttg ctgctctttg ctgcgttcct tcactctcaa acatgtgtac taaccagtct
    56101 tccatcaatt ctcgtagttc ttctgatact tttggcaata cttctttgat tgaacttgcc
    56161 aaagcctcgc gacgagtctg tgccgctaga agcataccat aaccaaattc agcattatct
    56221 tccaataaag aatttgacca tgcaggccct tgaccacatt cattggtcgt ataaggagaa
    56281 gcaggagaag caccgcccca aatagaagaa cagcctgtcg cattcgcgat catcattcga
    56341 tcaccgaaca tttgtgtcaa taattttaca taaggtgttt cgccacagcc tgagcaagcg
    56401 cccgagaatt caagaagcgg ttgattgaat tgtgtagaca agacagtatt ctttcctttg
    56461 actggatttg ctttttgtct taatgtcatt gcaaaagccc aattcacagc ttgttctttc
    56521 tgttcttcat aaggcttcat cactaaagct ttttcttttg cgggacaagc ctctacacac
    56581 agaccacaac ctgtacagtc ttccactgaa acttggatac ggtattttac gccgtctgcg
    56641 cctcgcattt ctctcacgat aaatccttct ggcgcttctt ccatttcctc ttcatctgct
    56701 aaaaatggac ggatggcagc atgaggacaa acaaaggcac attcattaca catcgtacag
    56761 cgatcactaa tccactcagg tacttctaaa gcgacgcccc gcttttccat agaagccgtt
    56821 cctaaaggca tttcgccagt tgtcattcca tttgctacaa gtgtaccaac agagagttga
    56881 tttccttcct gtgcattcac tggttcaagt atttcttgta tataaccagt gttcctttta
    56941 ttgatttttt caggcatctc caacgttctc caatggtctg ggatcgcgac ttgatgaagc
    57001 agttcggtcg tccgatcgat tgcttgtatg tttttttcta cgatagtcat tgatttatgt
    57061 ccatagcttt tcagggcttc ttcttttagc agcggaagca cctcatcaaa aggaatgatg
    57121 tcagctaatt taaaaaaagc cgtttccatt gccgtattga tccggcgtcc caaaccgact
    57181 tcttgggcca atctcatggc attgatcgta tagaatctaa tgttattttc agccaagtaa
    57241 cgttttaatt ttaagggcag atgcgtttct aattgttcgt cgctccaaag agtattcaat
    57301 aaaaaaatgc cacctggctt caatcctttg accaagtcat agctatgcag gtaagctggt
    57361 gtgtgacagg caacaaggtc cgcatgctcg acaagatacg ctgaacgtat cggcgtatct
    57421 ccaaaacgaa gatgagagac agtcagaccg ccagattttt tcgaatcata ataaaaatag
    57481 ccttgcgcat atttatcggt gtgatctcca ataatcttga tagctgattt atttgctcca
    57541 actgttccgt cagatccaaa tccccaaaac ttggcctgga atgtttgcgg ttctgtcaaa
    57601 tccagacttt cctttggttc caaagattga taagtcacat catccacgat ccctatggta
    57661 aaccgttttt tgcgtatgct tgggtctttt ttcaattcat caaaaactgc tgagatttgg
    57721 tcgggagtca catctttaga ccccaatccg tagcgaccac caataacagc cgggcgaagt
    57781 tcagaatcat ataacgcact ttgaacgtct aataagagag gttctccacc tgcccctggt
    57841 tctttacttc gatcaagaac agcaattgtt tttactgtat gaggcaactt ttgcacgaat
    57901 gtttccactg gaaaaggacg ataaagatgg atattcaaaa aacctgtttt ccgtccgttt
    57961 gcattcaagt aatcaatcgt ttgttcaatc gtctgtgcaa ctgatcccat agaaacaatg
    58021 acttcttccg catcgtctgc tccgtaataa gtcaccaagt cataagaagt tcctcgtaat
    58081 cggttgatct ctttcatata tttttgaacg atttcaggta atttatcata ataggggttg
    58141 atcgtttccc gttgttgaaa atggatgtct ggattctggt tcgtaccact aacagtcgga
    58201 tgattgggat tcatccctcg tttacgaaat gaagctaatt tctcctgatt gatcagagga
    58261 gctaattctt catattccaa aacttcgatt ttttgaatct cgtgactcgt acggaatcca
    58321 tcaaagaaat tcaaaaaagg gacttctcct tcaatagaag caagatgggc aacaggtgct
    58381 aaatccatca cttcttgtac actgctttca gctaacatag caaatcctgt ttgtcttgca
    58441 gccatcacgt ctccgtgatc tccgaaaata ttcaacgcat ttgtcgtcac tgcccgacta
    58501 gcaacgtgga aaacacttgg cagtagttcc ccagcaatct tgtacatatt cggtatcatc
    58561 aaaagcagcc cttgtgaagc ggtatatgta gttgtcagtg caccagtttt caatgatcca
    58621 tggaccacac cagctgctcc tgcttcggat tgcatttcta ctacttttac tggttgaccg
    58681 aatacgtttt cttttcctgc tgctgaccat tgatctacta attcagccat ggtcgaactg
    58741 ggtgttattg gatatattgc agcaatctcg gtaaatgcat aagaaatata tgcggctgcc
    58801 gtattgccat ccattgtttt catttttcgc atgattcatc atccttgttc atattctctc
    58861 ttattatctg ttccattata acgtaaaaca tgccattttt ttcttttatt tataaaaata
    58921 ttttacatgt atctatttga attggtcatc taatttttag acaaacagtc taaatcgttg
    58981 cctttttttg tcagacatgt ataaactata agcgaaagag gtgttttaac atggatatat
    59041 cagtgattga tgcaacgaaa acaaatacac aaaaaggaat cctttatggt tcatcgaatg
    59101 ctcctaaaaa aatggtcgaa ttcatcaact tagcttgtcc gtattgccga caatggtttg
    59161 aagaatctta tgagctatta gaagaagcag tccaaagcgg gcaattgcag cgagtgatca
    59221 aattgttcga taaagaaaaa gaaagcttac agcgagggaa cgtgatgcac cggtatctca
    59281 caatcagcga tggccaaaaa gcgatcaaag agatcaaaca aatattcgat actcaagacg
    59341 agtggaaaca tctttctctc caagaagtag cggattttgc agtagataaa ctaaagctga
    59401 cagaacaaaa agacgaacag ctgagccaag ctgtcatcaa cgaagctgag caagcacata
    59461 ttcgatttgt tccaactgtc atcttgggta aagaaatctt tgatgaatct atttcaatag
    59521 aggaattaaa agaattgatc caaacaaaat aaaaaaataa ggagcgtggg acaaaagtaa
    59581 agaacacttt tgtctcacgc tctaaacacg tataaacggc ggaagcagaa gcaactcctt
    59641 cttatttctt ggagttaaac gcttctgtcc cagcctcctt attccgaata aatggtgtcc
    59701 aaaagcttgc atctgccaga ttatctcatt tcttatgaca agctaagcaa attatctcca
    59761 ccaaataatt tattcatatt tttgacttgc gcatactaac taattcttaa attttcatac
    59821 aaaatttctc aatttccctt aacgagtaat atttatgtac ggtgattctc actagtatcc
    59881 caatcacttt ttaagttatc ccgatttcaa ttctacttca aaaatagttg cttttatgta
    59941 tgctgttagt tgattagatg gtcgtttgtt gagctaggtc gtttatttta aacaagttgt
    60001 ttctttagct tatacctatc tcgtctacca aacgcaagat ggcaaacaca aatgcatacg
    60061 catctgtatt tgccactttg attcgtataa agctaacaac tcctaactcc tctcatttaa
    60121 tagttgctct cttttttatt cagatacatt tttgctgatc acagcctagt atttcaatgg
    60181 ttttgaacac taaaccaaat tgttccatta caacattcac tggctgatga aatggattaa
    60241 tagatttcga ttattcaaag cgattttcta ttggtggatt gtgttcccaa ctaacaagac
    60301 ggattccaaa gtcattatat aactcttcaa gagttaaccc tttcctgttc ccaatagatg
    60361 tatctctccc acgctcgata taaaatatgg agtttcgaat gccaagacct ggcaaatctt
    60421 tccttgttac agttacactg ctactaactt ccccatataa aatccagctc ctccaaaatg
    60481 ataatatatt tttcgaccat catctaactc atgtaacaat aattgttgtg ccctcttgtc
    60541 actcgccaaa tgcaaacgcc acgtgcgata ccatgccatg ctacctgcat ctgcagaaaa
    60601 tccatcaaat aatgcaacaa ttgtatcttc tatcacaatc ctttccccat ctacttcata
    60661 ttctaatcta aaatcaaact cccctcgggt aatcgcaggt cttggtggct taggacccaa
    60721 tattattcta cttaaaaccg cattcgttcc cagatgaaac acagctataa caacaataat
    60781 gattaatatt ttgtttctat ttgataaatt tctcattatt cgcaaatcac ctctacattc
    60841 tcacatacta gttcgtaata ctcctttaat atcaaaatat ctactgatcg taataatatt
    60901 cgctatgaat tcctactact cgtataagaa aggccaaatc atcagctttt acttgctgat
    60961 gatttggctt gtttctattt atcgtaattg ttttctaatc accaaaattg ttaacaatcg
    61021 gttcagatat ttcccaattc actagcctta tattatattg atttaacaat tcttcttcat
    61081 ttattgtatt ttgatcaagc ggaccaaatt gttctaataa ttctttgtca tgtaaaatat
    61141 cccttctagt aatgcctctt tccctttcaa tgaaagaagc actaggaaaa gtatgattat
    61201 aaccattcaa cctatctccc atgtaataat tagcaggtcc tggaaaataa aatatttgct
    61261 tattatcact aacttctaac agcaacacta aatcttctcc gctggctaaa cgacttgtcc
    61321 atctacgata tctccctatg ccttcgttca ttccaaatcc atcaaattca actattaacg
    61381 tgtcttctat cacatgtaat tcttcattca attcatactc aagtctaaaa ggaaattctc
    61441 catatgtaat ctctggcaat ggtggtgctg gagttctcca aataaataca ataactgtga
    61501 atagaaaaaa tccaacgatc gatataaatg ttatgagacc tgctcgaagt aaaacccgat
    61561 ataataaaaa tgctgtattt cgctttgcta tatctagaaa aataaaaagt actgcaatca
    61621 taatgatagt aaaagttgta aaaaaacgca tttttgccct ccctttataa acatcgattc
    61681 tcaaattcta aaccaaatct tggtagttca agttcaggta taacgtattc caaccataca
    61741 tctcaattac tatttatgaa cttatgatta tcactgaata aatcactcca tccccttgtt
    61801 aaaacttcta cattaattac acctggttgt tttcgactag ctaatccaat gaacttatca
    61861 accatcctta tccaccaacc acgagaatct tgaccagaag taagaagagc agttctcaag
    61921 gatgatcgag cagttccttc cagtttttta attaatcagg ccagttatag ataaatactt
    61981 gatcttatct atatcaggac ttctatatta agcaaaaatt tatgtacaaa actaaaatct
    62041 tcattcagtt gtctccccgc tttttatacg attgtattaa aaacaataga agcctttcca
    62101 aattatgtta ctccagtaaa gatggaatta tagtttgacg gaagaaaagc aagtataact
    62161 tttaaaaaaa actgatacat tcgcatctcc tgcttcatgt atcagttttt tctttggatt
    62221 aaactgcttc ttcacgcagt ttttccgcaa attcatttat tttgcgaatt cggtgattaa
    62281 ttcccgattt tgatatcgca cctgagggaa tcatttcccc taattctttc aagctgacct
    62341 ctggatgttg aagccgaagt tctgcgattt cctgaagttt ttcaggaagg gcatgtagac
    62401 cgacctttgc ttcgatcaat tcgatgtttt cgatttgttt agaagccgca tcaatcgtct
    62461 tgttcagatt tgctgtctca caattgacta gacgattgac cgaatttctc atatcccgta
    62521 cgattcgaac atcttcaaat cggagcattg agttggttgc accaatcaat gttaagaaat
    62581 cagcaatttt ttctgctcct tttaaataag aaatatagcc attgcgccgt tcaagcgttc
    62641 gagcatgtag accgtaataa tttaacatat cgcaaatgtc ttggttatgc tgttcataaa
    62701 tagaaaatat ttccaaatga taacggctcg tttccggatt attgactgaa cctgaggcca
    62761 taaaagcacc acgtaagtaa gaacgcattt tttgcgaatt tcccataatc tcgtccgaaa
    62821 cgtgtccatt gaacatcata ccatccatga tatcaagatc catcaacaca tcttttgtgc
    62881 cttgcttcaa ccggacgata tagacattgt tcttttttaa tttcattttt ttacgaacga
    62941 gtagctcact tcgtacctga taatgatctt tcaaaagaga atagatacgt ctggcaattg
    63001 cagcattttc tgtttggacg ttcaaaataa actgctggtt cacaatacct aaagagccgt
    63061 tcattcgcag caaagcagct aattccgctt tcgcatgttc tcgatgaact tccaaaccag
    63121 ttagttcttt cttcacatct gcagcaaatg acatcctatt ctcctccttt ctccaacaat
    63181 aaattgctta atattttgaa ccgaaaacta aacggaacaa ttcttctact actttgtctc
    63241 catcgtggaa gacaccacca tctttcaatt ccaaaaagtc ggtggaaatc actcgacatc
    63301 cttcatttct caatccgttg aagtcgtgtg cgacttgtac tagatactca tcatagatct
    63361 ctgggtccat ataatcttca ggcacttttt ccgtattgac caatactgta tcgataaaag
    63421 gctgattcaa atgcttgtgt aaaacacgta tatgatcggc atctgtgaaa tgttccgttt
    63481 cccctttttg ggtcatgata ttacagatat agacggtttc agcagttgtc ttgagcattg
    63541 cttcgccgat ttctggtatc actaaatttg gaaggatact ggtgaataaa cttccgggtc
    63601 ccaaaacgac catgtcggcg tccataattg ctgaaacgac ttttcgagcc gctttggctt
    63661 cacttttatc atttgtgttg cagacataca cacggtcaat ggttttacgg tcgatcgcga
    63721 ttttagattc tcctactgca gtcgttccgt ctttaaagac agcatgcaag gtcaaaggag
    63781 tttctgaaga aggataaaca tgtccatcta catgcatcat ttttgctaat aattgaatcg
    63841 cctcataagt gcttccccgc atctcggaaa ctgcggcaat gatcagatta ccgatcgtat
    63901 gattggctaa aaactgatct tcctttttga aacggtattg gaaaatatct tcatagaatt
    63961 gtggcatatc cgataacgcc actaagacat ttctcaagtc accaggagga gccatagcga
    64021 tcgattctct gattgcaccg ctgcttcccc catcatctgc aacagtcact actgctgtga
    64081 tatctgctcc ttgatttcgc aaacttttca ggataactgg cagaccagtg ccaccgccga
    64141 ccactacgat ctttggtttt cttatccggt aggttttcat tttcacgacc ggttcaccgt
    64201 ctctttcctt ttatccttgt cccgatgtgt cacattgact ttatagtttt tccctaaagc
    64261 atttgccaaa cgttcagtca atgccacaga acggtgttga ccgcctgtac agccgattgc
    64321 aactgtcaag ctgctttttc cttctttaat gtatccaggc aatacagttt ccagaagatc
    64381 caaaaattta tggtagaatg attgcgtttc atcaaaggac atcacataat catagactgg
    64441 ctgatctttt ccggtcaatg gtcttagttc cgggatataa tgagggttgg gcaaaaaacg
    64501 cacatccatc acaatatccg aatcgatcgg caacccgtat ttaaaaccaa atgatacaaa
    64561 ttcgatccga aatccttggt ctccctttga tttgaatgac tcattgattt tttcacgtaa
    64621 ttgtcgtggt gtcaatgtcg tcgtatcaat gatcacggaa gcttgggctt tcaagtcgtc
    64681 tagtatcgcc cgttctttac gtatcccttc agtgatcaaa ccgtccatcg ccattggatg
    64741 tgctcgtctt gtttctttgt agcgagccac tagttcttcg tctgaagcat ctaaaaataa
    64801 gatagaagtg tcgatgaagt tcgtattttc gatttcgatc agcatgtttt ggatttcttc
    64861 aaagaaggaa cgagaacgta aatccactcc taaagccatt tttgtgactt tgcctgattc
    64921 tttgattaat tcccagaatt ttggaatcaa tcgaggcggc atattatcga tacaaaaata
    64981 gcccatatct tcaaaacttt ggatagctac tgttttccct gcaccgctca tacctgttat
    65041 gatgactaat tgcaaattat ctgtcataat tacgaactcc tctcacattg tgcatagtaa
    65101 aattatatca ttccgggcgt atcattacca gttttctttt tcttttacaa aaagatttgg
    65161 accaaatatc ttttaggata tttgccccaa atccaatttt tttacttttc cgatgctgta
    65221 tccaatgcct tgcttctttc agctagcgct cgttcaatcg tccgatctgc taaaatagat
    65281 tgggcatcga tcactggata atggttatct tcagcaaatt catgcatcaa tgacaattct
    65341 gtgcaaccta agataacttt ttcacagttt aggcgctcta ccgcttcttc taagatttcg
    65401 taatatagct cttgattcaa ataatccgat tccttgatct catgatagat cagataattg
    65461 attttttctt gaagaacagt atcaggaatc actgtttcga atccaagatt tttgacttcg
    65521 cgttcataga ttccagcttt catacttcct tctgttccta gaatcgctac tctccctgtc
    65581 gtatgctgac ggacaagttc atttgctgct tctcttggca tatgaaggat aggaatatcc
    65641 gttgccgctt gtaattcttc aaaaaagtag tgtgcggtgt tacaagtcaa cacaatgaag
    65701 ttcggctgca ataaattttg tttctcaata tcatccagca gaaacggcat tggattttct
    65761 tctgatcgat ctaaaatata agctgtacga tcaggaactg ttgcatgatt gaataagaca
    65821 taattcaaat attcttgatc ttttgtagct ttcgttcggt ggttgatcaa gcgaacaaaa
    65881 ctttctgtcg ccatcgtgcc cattccgcct aaaatactga aaaaattctc catgaactag
    65941 ccttctttca catgaaaata ctttttgaat cttggaatat agttatggaa catatatttc
    66001 atcaacaacc agcgcttgat tgaccggtct ttttcataaa acacagtagt accgtaacga
    66061 ttttctttga tcatttgctc agctagtttt ttgtcttcat tttcccgagc atattctaaa
    66121 aagatttttt tgggcacacc catccataat cttgctttat ctgattgatt cgttccatag
    66181 gtcgtttcaa caaagggttt gttaaacact cgatcttctg tgacgaaacg agccagattc
    66241 aagccgttca atgtcacaaa gaaactgcta cgaccttgtc tcaaattgat ttcaaataat
    66301 ttatattctc catctctagg atcatacttc atatcgaaat tggcaaagcc tgtatattcg
    66361 attttttcca aaaatgcctt aatggtttga tagatttttt cgttataatc tggcatgatc
    66421 acaacgtaat taccaatcga tgctggtgta ggatcttcta acagtggatg accaagacac
    66481 atcatccgaa cttgatgatc ttcatctaca tacgcattta gcacacgcat attactatca
    66541 tcacccggga taaaatcttg tacaatcatt tcacttttat aaccggcttc gtatatccgg
    66601 cctaaaatta aatcgaactc ttctctagtc tctaaaataa aagctttttt tcttccttca
    66661 aattggacag aaagatactc cacactgttt gcaggtttca aagcaactgg gaattcaaaa
    66721 ggcaattcct gttccaaatg tccatttact aacatttctt ctctgacgat caatgtttta
    66781 ggatacggaa gatcatattc ttcacaaact tcataaaagc ttactttatt gatcagtctt
    66841 tcaaataaag aataatcaat atatggacag ataaaatatt ctgacagctc ttgtttatgt
    66901 tgggagatca attctgcata tccatcacca caagcaatca aaagatattt tttactctta
    66961 tcagaatatc tttcttttgc caaacgaagc atcgtctcga taaacacagg gtctttatca
    67021 aatcctggaa taacttctac attgacaatt ttactgtagc gggtaggggc taactgatcc
    67081 gaagcatatg cttggcagat tttgccgtat gcttcattaa aagaacgtgc cataccataa
    67141 acgttcatgt cgcttcctaa taatataggg ataaattctt cattttcaat actgttcatc
    67201 attatctctc acttactcat aatctcttgt ttttcttttc caactacaca aggaatatca
    67261 taccataaag aaaaaagcaa tcaaaggaaa ctcctccgat tgctcaattc ttttttgaac
    67321 ggattgaact tataggaagt ttttgtctat accaatcagt gtagaaagaa ctaccatgta
    67381 atgattctag aacactgata ttaataaaac aagcaaaaat tcattttttt atgttttttt
    67441 aactatctca gctatctatc ctctttatac cattattttt ttcaaatagt gacctgtata
    67501 actttctttt acgttaatga tttcttctgg tgttccagtc gcaaggatcg ttcctccacc
    67561 ttctccacct tctggtccta aatcaatgat ataatctgct gttttgatta catctagatt
    67621 gtgttcaatc actaaaactg tgttaccagc atctactaat ctttgtaata catgcaacaa
    67681 gcgggcgatg tcatcgctat gaagtcctgt cgttggttca tctagtatat agaaattctt
    67741 tccattagag attttgtgca attcactggc aagtttcatc cgttgtgcct caccaccgga
    67801 caatgtcgtt gctggttgcc ccatcgtcac ataacctaag ccaacatcaa caatcgtttg
    67861 cagtttgcga tgaatctttg gaatgtgctt gaagaattct acagcatctt ctactgtcat
    67921 ttccaaaata tcagcaatgc tttttccttt gtaatgcact tctaatgttt cagagttata
    67981 tcgtttgcca tgacatactt cacaaggaac atagacatca ggcaaaaagt gcatttcgat
    68041 cttaataatt ccatccccgc gacaagcttc acaacgaccg ccttttacgt tgaaactaaa
    68101 acgccctttc ttataacccc gcatttttgc ctcgttcgtt tgagcaaata acccacggat
    68161 atcatcaaat acacttgtat aagtcgctgg attacttctc ggcgtccggc cgattgggct
    68221 ttgatcgata tcgatgatct tttcgatact ttcgtagccg gaaattgtct tgaatttacc
    68281 tggcttagca gaattcttat ttaatttttg cgctaacgat ttctttaaga tactattgac
    68341 caatgtactc tttccagaac cagacacacc tgtgactgct acaaattctc ctaatggaaa
    68401 ttcaacacta atatttttca gattgttttc tgaagcacca gtgatcttga ttgctttccc
    68461 attaccttta cggcgttctt ttggtacagg gattgatttt ttccctgaaa gatatttccc
    68521 tgtcaaagaa ttttcatcat tagctacttc ttcaggagtg cctgcagcca cgatttgtcc
    68581 gccttgttct ccggccccag gtccgacgtc aatcagataa tctgctgccc acatcgtatc
    68641 ttcatcatgt tcgactacaa cgagggtgtt gccaagatca cgcattttct ttaatgagcc
    68701 aatcagtcga tcgttgtcgc gttgatgcaa tccgatcgat ggttcatcta aaatatacaa
    68761 aactccagaa agattcgatc caatttgtgt agccaatctg attcgctgcg cttctccccc
    68821 agataatgtt ccggaagcgc gactcaaagt tagatagtct agtcccacat tttccaagaa
    68881 tgaaagtctg tcatttactt cttttaaaat cggtttggca attactttct cttgttcaga
    68941 taacgttaag tttgagaaga aatctactgc tttttgaata gacaactcat ttgtttgccc
    69001 gatattcgtt ccattgattt gaacagatag agcttgggga ttcaatcgat aacctttaca
    69061 tgtctgacaa gttaattctg tcatataaag acgcatttgc tcacgcgtga aatcactgtt
    69121 cgtatcatga taacgacgct cgatattctt caatacccct tcgaaagtcg tttctacatc
    69181 gcgaacgcca ccaaaatcat tttcataatg gaaatggaaa agatcatctg tcccattcaa
    69241 gatgatatcc tgatgctctt ccggtaattc ttcgaatggt gtatctaaat cgataccgaa
    69301 actttcacaa gcctgcgcta acatttgagg ataatactga gagctgattg gattccatgg
    69361 aacgatagca ccttgactaa gtgtcttcgt tctatctgga atcactaaat cttgatcgac
    69421 ttctaatttg acacctaaac catcacagtc tggacaagct ccaaaaggcg cattgaatga
    69481 gaacagtctt ggttctagtt cgccaacagt aaatccgcaa taaggacagg catagtgttc
    69541 gctaaacaac atttcttctt caccgatcac gtcaaccaga gcatagccgt ctgccaatcg
    69601 cagagccgcc tctaaagaat caaatagacg agaacgaatc ccttctttca caatgatccg
    69661 gtcgatgacg atcgcgatat catgtttttt gtttttctcc agctcaggca cttcacttaa
    69721 atcatagatc tcaccatcga ctcgaacgcg gacgtatccc tctttttgga ttcgttcgaa
    69781 tattttttta tgttgtcctt ttttctttac cacgatcggt gccaacactt ggatctttgt
    69841 gcgctcaggc aaagccatta cttgatctgc catttgatct actgattgac tggtaatctc
    69901 gatatgatcg ttgggacaaa tcggatgtcc tacacgtgca aacaacaagc gtaaatagtc
    69961 attgatttcc gtcacagtac ctactgtcga ccgaggattt ttacttgttg ttttctgatc
    70021 aatagaaatc gccggactca atccgtcgat gctatctacg tccggcttat ccatttgtcc
    70081 taaaaactga cgagcataag cagaaaggct ctctacgtat ctccgctgcc cttcagcgta
    70141 cagcgtatca aatgctaggg aactttttcc agaaccggac aaacctgtaa cgaccacgaa
    70201 tttatctcgt ggaatcgtta catctatatt tttcaagtta tgggcacgtg caccgtgaat
    70261 cgtaatttta tcgttagcca tgttgtcctc ctactttgaa gcttttaatt ctaaaatcat
    70321 atctcgcaca ttagcggctg tttcaaaatc caatgctttc gccgcatttt tcatttcttt
    70381 ttctaatttc atcaataacg tatctttttc ttctttcgtc atttcttcat acgagacttg
    70441 taccgcttct tttgtatcat cttccgattc tttcgtaata gaaatcaaat ctcggatttc
    70501 tttaatgatc gtttttggaa cgatcccatg ttcttcattg tacttctgct gaatcgtacg
    70561 gcgtcttgat gtttcatcca tcgctagacg catagaatct gttactttgt ctgcgtacat
    70621 gattacttta ccttcttcat tccgagcagc tcgtccaatc gtttggacta aggaacgttc
    70681 actacgtaaa aagccttctt tatccgcatc caagatagca acaagggaaa cttcaggtac
    70741 gtctaatcct tcacgaagca aattgattcc aactaacaca tcaaattcac ctaatcgaag
    70801 atctcgaatg atttctgttc gttccagcgt tttgatatca ctatgcaaat atttgacttt
    70861 gatccctaat tctttgaaat aatctgtgag gtcttctgcc atttttttcg tcaatgtcgt
    70921 tacaaacacc cgctggtctt tttcgactcg ttcatttatt tcgcctacta gatcatcgat
    70981 ttgtcccatg attggacgaa cttcgatcac tgggtcaagc agccctgtcg gacggatgat
    71041 ttgttggata acggtatctg tttgttcatg ttcatatggt cctggcgtcg cagagacata
    71101 aatgatctga ttgacatgtt tttcaaactc ttctaaccga agtggccgat tgtctaaagc
    71161 agatggcaaa cggaaaccgt aatctactag catctgtttt ctcgctcggt caccattata
    71221 cattcctctg acttgaggca tggttacgtg cgattcgtcc gccacgatca aaaaatcttc
    71281 aggaaagaaa tccaatagcg tataaggcgg ttcaccttct ttacgtccgt ccatatgccg
    71341 agaataattt tcaattcccg acgtgtagcc catttctaac atcatttcaa tatcgtaatt
    71401 ggttcgctgt tctaaacgtt gagcttccag aagtttgttt tcatttcgca agacagttaa
    71461 acgctgttct aattccgctt tgatattcgc tacggcatgc tccatatgtt catcattcgt
    71521 cacaaagtga gtcgccggga agattgccac gtgttccgtt tctcctaaaa cttcacctgt
    71581 cagtgcattg acttctcgga tacgttcgac ctcatctcca aaaaactcca cacgtaaggc
    71641 acgttcatct cttgaagccg gaaaaatctc cacgacatca ccgcgcactc gaaaacgacc
    71701 gcgctgaaaa tctatatcat ttctttcgaa ttggatactg actaagtcac gaatcaactg
    71761 attgcgatct agttctgctc cctgtcgaat cgaaactact tgtttttggt actcaaaagg
    71821 cgaacctaat ccaaaaatac atgaaacgga tgcaatgacg atcacatcat ttctttctaa
    71881 taatgaacta gtagccgaat gtcgtaattt gtcgatctca tcatttacac ttgaatcttt
    71941 ttcgatatac gtatcgcttg aaggcacata agcttcaggc tgataataat cataataaga
    72001 aacaaaatat tcaacagcat tgttagggaa aaattccttg aattccccgt acagctgacc
    72061 agccaatgtt ttgttatggg caataattag tgttggttta ttcactttct caataagatt
    72121 ggaaatcgta tatgttttcc ctgttccagt cgcacctaat aagatttgtg cttttttccc
    72181 gccgactacg ccatccacta attggttaat cgcttcaggc tgatcaccag ccggttggta
    72241 tttcgagaca agctcaaagt gtcgggaggt atctctctca atcattcttc gtcctccttt
    72301 tcattcattc cttttcattt tacgaccaat aaaaagaaaa ggaaagcaaa ggcattcctt
    72361 actaaaaaat tataccacac cgaacatact ttcgccactt ttttatctta ttatgcatgt
    72421 tacgttttat aacataaaat gttagaaact cgcatttttt tcattttttc gagcttacag
    72481 tcttgtctga ttcagcactt ttcgcctata ataataaaat attggaaatt taggaggttg
    72541 attatgaaaa gaaagcattt atttcactca tttgttttct taatgacagg acttgtgaca
    72601 cttgcgtttg gacatgttgc acaagctgac aacactacaa cggcagagtc aagcacaagc
    72661 gagacaatgc aaaaaataga gcccaaaaaa gacgtatacg taattgccag tgactctgct
    72721 tttgctccat ttgaatttca aaacgcaaag ggcgagtacg aaggcatcga tgtaaaattg
    72781 atggaaaaca tcgccaaact gcaaggattt aacatccgaa tggactaccg tggtttctct
    72841 gctgctttgc aagctttgga atcaggacaa gcagacggaa tgattgccgg aatgactgta
    72901 acagatgaac gaaagaaaag ctatgatttt tctgattctt actttgatag cgggatccaa
    72961 ttagctgtca aaaaaggaga tacttcgatc aagtcttatg aagatttgaa aggaaaaaca
    73021 gtcggagcaa aagttggtac cgaaagtgct gaatttttaa gcgaccatga atctgaatat
    73081 ggctattcta tcaaatattt tgatgctgct gatcaattgt acgacgggtt aaaagttggc
    73141 gcaatcgatg caatgatgga tgattatccg gtaatcggtt atgcgatcaa acaaggacaa
    73201 gaattggaaa caccgatcaa aaaagaatct ggcggaaaat atgcatttgc cgttaaaaaa
    73261 ggacaaaatc ctgagcttct taatatgttc aatgaaggat tgaaagaatt aaaaagaaca
    73321 ggcgaatacg ataagattat taaagaatat gtagcggatg gttcagaaac ttccagtgag
    73381 tcttccactg ctgatgaatc aacatttctt ggacttatcc aaaacaacta tcaagcattg
    73441 ttgaaaggac tttggagaac aattctccta actttgatct cctttgcttt ggctatagta
    73501 atcggtgtga tttttggatt attcagtgta gcaccaatac gcgctcttcg cacaattgct
    73561 agtatttatg tcgatatcat ccgaggaatt ccaatgatgg ttcttgcgtt ctttattttc
    73621 tttgggttgc ctggtatcgt tggatttacg attcctgact tccttgctgg tgttatcacc
    73681 ttgactctta acgcaagtgc ttatatcgca gagattgttc gaggcgggat caacgccgtt
    73741 cctgtcggac aaatggaagc ttccagaagt ttaggtcttt cttataatcg gacaatgcaa
    73801 aaaatcattt tgccgcaagc gatacgcatc atgatcccat cattcgtcaa tcagttcgtt
    73861 atctcattaa aagacacaac gatcatttcc gcaatcggtg tagtcgaatt gctgcaaact
    73921 ggtaagatca ttgttgcacg tacaacacaa agtacgtatg tttacttgat cattgcgatc
    73981 atgtacttga ttttgatcac agcattgaca aaattagcaa aagttttaga aaagaaggtg
    74041 aagtaagatg gcagaaaaaa tcttagttga gcatttagtc aaaaaatacg gcgataacac
    74101 agtattaaac gacatcaatg tatccatcaa cgaaggagat gtcgtctgta tcattggccc
    74161 ttcaggttca ggaaaaagta cattcttgcg ttgcttgaat cgtttagaag aaccttctga
    74221 cggtgaaatt attattgacg gcgcccactt gatggataaa agcacagata tcaacttggt
    74281 gagacaacat atcggaatgg tattccaaca ctttaaccta ttccctcatc tttctatttt
    74341 ggaaaacatc attttagcac cgatggacct gaagaaagaa tctagagaag aagcagagaa
    74401 aaaagcaatc aaattactgc agacagttgg tctagaagat aaaaaagata tgtatcctga
    74461 aatgctttct ggtggacaaa aacagcgtgt ggctattgct cgtgctctag cgatgaaccc
    74521 tgacatcatg ctttttgatg agcctacttc tgcactcgat cctgaaatgg tcggtgatgt
    74581 cttgaacgtc atgaaaaagt tagcagaaca aggtatgacg atggttatcg ttacgcacga
    74641 aatggggttc gctaaagaag tggccaatcg cgtcatgttc attgacgacg gaaacttttt
    74701 agaagatgga acgcctcaac aaatctttga aaatccgcaa aatgaacgga caaaagattt
    74761 cttgaataaa gtattaaata tttaaaataa attatatatg tctttttgac tatcttttta
    74821 tatcttctta aatgtccgca aagccttatt ttataggctc tacgggcatt ttgcttttgt
    74881 ggtaaacctc acatatctag gtctatcttc ttatattttc actatcaagc gtggttaaaa
    74941 tcgtggtaaa tacttctatg caattagacg ctgaacctct gattttgcgg tatctatgga
    75001 cgcatgagca taccagttca ttgtgatact gatatttgaa tgccccatga tatactgtaa
    75061 atcttttggg ttcatgttct tgcttgccag tcttgtgcag aatgtatggc gtagcgtatg
    75121 cggtgtgata tgtggcaatg gattgtcctt gtggtgcttg ttgtatttct ttaccatacg
    75181 gataaataag gcgttataat caatcgcaac tttgggcttg cccttatgat tgacaaatag
    75241 gaagttgccc cgtccgtcta tcacaaatgg ttctgccttt ggacgttttt tcataacctg
    75301 ttgaaatgcc tgtatcgttt ctttgcttaa tggtacttgc cttattccgc tttttgtttt
    75361 aggcgtttca atataatagc cctgttcctt gctctttagt aactggtggt cgataatcac
    75421 aacttcattc ttgaaatcaa tgtcggctac tgtcagtccg cacagttccg aaatacgaag
    75481 tcctgtcttt aacagtatca gcacatcatc ataatgcttg tgatacacat tgtccgtctt
    75541 gatgaatgag agtaaggctt gttcctgttc ctctgtcaat gcgactttct ttttggtatc
    75601 attttctagg acttcactca acttgaaatc aaagggattt ttcctcacat aatcatcttg
    75661 aatggctatg tagaatgacg cttttaacga gcgtttatgg ttgttaatgg tattgtagga
    75721 aaagcctttg tctttcatgc gtaacgccca ttccttagcg tcagagggtt tgattgtatc
    75781 aatgctccta gcacctaact tgtcctcttt caataaccgc atgagttgtt cccgttgttt
    75841 ctgtgtgctt ttcttcacat ttgccctctg tgcgttctgt ttggcataaa gttggcaaag
    75901 cgtcattttc ttgccagtgc tgtcgatacc gtcctcaata tcccgtctta tctgctgttc
    75961 cagttctcga agtgagggtt tttcccgttt tccctttggt gtcgggtctg tgggtgtcaa
    76021 tctccaagca tacacatatt ttgtgtttcc aaatgcgtct acatatttat ataagtattt
    76081 tctgtctgtt cgttggctct ctccagtatg caggatacga cctttgttat cccgtctttt
    76141 ttctttcatg gtgtctgctc ctttccttgt tggaaagagc cttgatatga cttgtgttca
    76201 tcataacaca tacaaggctc atttgcatta gatagcgtcc aatttatcaa tgacctgttc
    76261 aaactgtcgg cgtttaatct gtatgcggtt gccattcata atgagccagc cagcgtcctt
    76321 gttttcctct gccaatcgtc ttaacttgtt ttctccgata cggaaatact ttgacgcttc
    76381 ttcaatggta agggtgtatt tttcccatac gggaatatcc tgtgcattac tcataaaaca
    76441 ccccctcact ttcgattacg tctgctatgt ccgtacacat ctgttccaca agcatttcct
    76501 gcttttcaga gaggtgggta cattcatcaa tcattttcat gtagtttgag cctgtcagcc
    76561 tgtcgatttc ctctatcatc acgattgacg gcatgacctg tctttccagc cattgcagag
    76621 tgcgttccat attgacaggt acggggttca tttcaaaagg taaactgtca tgggaaatga
    76681 atatctccca cagcggatag tcgggaaatt gcacaaaatc agtaataagg taaaacacca
    76741 gcccatgagg gttatatgtc aataacaatt cctcaactgc ctgtactgct tttgtaccac
    76801 gcagacgaat ttcaaaacga ttgataatat ccgtatgttt ctttttggtt gcctgttcct
    76861 tttgtttttc atacaggcag aagtattttg tgcttgcctt tgaaccgata taaagggtgc
    76921 ttgctaaatt acggtttttt ccgcttagtt ttccaccctg cacattttca taatttttac
    76981 agcgacaatc cgcaccgcca tttctgtatt tttcagacaa aacgggaata tccaacagac
    77041 cacacatatc attgattgcc aagtcaaaac gtcggataac accgtcacag tcaagacagc
    77101 ggtttaaaaa tgaataccaa tctctgttct gtgcctgcaa gacatattcc atgttgcgtg
    77161 agcctgcccc ttttaattcc aagaatacgc ccatgtgttc gtcatcagaa gccatgacct
    77221 tgatttctcc gtaggcgtaa tgctctttat agccataata cccatagtca taatggataa
    77281 aataatctgc tttcatgcca agcacctttc ggataatttc caatgcgtca gtcgttggat
    77341 aacggacaga cagataatca acgagcagat aaaaaggctt atcgcagata taataattca
    77401 gacaacgcag gataatattt tgtgtttcct tgctggcgtt ggcttttccg ttctcaaagc
    77461 ggttgatact atatcttgat acatgggtaa gacttgcaag ttctccttgt gaaagaccgc
    77521 ttgcaagacg tttgttcttc atttcaagtg caaattctaa attgttcatt gtattcctcc
    77581 aatcccacaa tagccttgaa atacctgtaa acctctattc aaaaagtgtg atatgcacta
    77641 tcttcttaaa acttctcatt actactcaca actttgggat tgtatggttt ttgacttgtc
    77701 ttttgtgaaa tagtgccccc ctgttagata acgggggcac agacaaggct cgcaggctcg
    77761 ccagccgaca aagcatttct tccccttgac acaaggtgtc atggcggtaa gcctgagagg
    77821 cttccgccac cgtgtcagaa atactttgtc agcaagttct gttttgcctt ttctaacaag
    77881 ttccatttca accgatattc atatctacgg atttaatcag ataggtattt ctttttgaat
    77941 gtttttatat ttcattctat tgtgcttatt cgattacaag aacatagtaa ttcttatatg
    78001 cttatttgtc aatatctttt tttgccttta caaatcatat taagcatgat agacttataa
    78061 tagcgaagat ataacgagat atggagggtt atgcaatgaa aataagccat ttaggaaata
    78121 atatacagac cataagaaaa tttaggggaa tgaaacaaca ggaacttgcg gacaaaatcg
    78181 gtatcaatat gcagagcctt tccaagattg aaagagggtt gaactatcct gcctatgaaa
    78241 cgctggaaaa gataatggaa gtgctggacg ttacgcccaa tgaattgttg tcgggagaat
    78301 ggaagtatgt caatcaatct gaaaaggaag tctgtcagtt tttaagagcc gaagaacgtc
    78361 taaatgcaga actgaaacat ggacactatg ataacttctt tgacagcgag gaagaatggc
    78421 tggaatatga gttagaaaag ttacgggaat atattacaga ctatatcaac gggaaaagca
    78481 ttgaagcgtc cgacctttac ccaatcaaag aatttatcca gcatttgaag tttcaaaaat
    78541 tattagaccg ctatgatgat ttatacagta tggatatgtt tggggaaagc atagagggac
    78601 ataagtataa gacaccttat caagtcgtga aaatgataaa cccgaactca aaagaagata
    78661 tggaactgtt acgagaagta ttaaggaata acaattttga tgatgaggac gagtaacttt
    78721 tttcctcttg cgagaagata cagcaaacaa aaagcccgat caagagtgca aagcaccacc
    78781 aatggtggca aagctcttga cagggctttt tcttttctgt tgtgggcaat caagaggaag
    78841 aaagggataa aatctgattt gtggtaaatc aggcaaaggc aacgcaggta tctcaatttg
    78901 atttggtact tgaaaagaac gggagtaatt ggaagattat agaataacaa atattggtac
    78961 attattacag ctattttgta atcacgtact ctctttgata aaaaattgga gattccttta
    79021 caaatatgct cttacgtgct attatttaag tatctattta aaaggagtta ataaatatgc
    79081 ggcaaggtat tcttaaataa actgtcaatt tgatagtggg aacaaataat tggatgtcct
    79141 tttttaggag ggcttagttt tttgtaccca gtttaagaat acctttatca tgtgattcta
    79201 aagtatccgg agaatatctg tatgctttgt atgcctatgg ttatgcataa aaatcccagt
    79261 gataaaagta tttatcactg ggatttttat gcccttttgg gtttttgaat ggaggaaaat
    79321 cacatgaaaa ttattaatat tggagtttta gctcatgttg atgcaggaaa aactacctta
    79381 acagaaagct tattatataa cagtggagcg attacagaat taggaagcgt ggacaaaggt
    79441 acaacgagga cggataatac gcttttagaa cgtcagagag gaattacaat tcagacagga
    79501 ataacctctt ttcagtggga aaatacgaag gtgaacatca tagacacgcc aggacatatg
    79561 gatttcttag cagaagtata tcgttcatta tcagttttag atggggcaat tctactgatt
    79621 tctgcaaaag atggcgtaca agcacaaact cgtatattat ttcatgcact taggaaaatg
    79681 gggattccca caatcttttt tatcaataag attgaccaaa atggaattga tttatcaacg
    79741 gtttatcagg atattaaaga gaaactttct gccgaaattg taatcaaaca gaaggtagaa
    79801 ctgtatccta atatgtgtgt gacgaacttt accgaatctg aacaatggga tacggtaata
    79861 gagggaaacg atgacctttt agagaaatat atgtccggta aatcattaga agcattggaa
    79921 ctcgaacaag aggaaagcat aagatttcag aattgttctc tgttccctct ttatcatgga
    79981 agtgcaaaaa gtaatatagg gattgataac cttatagaag ttattactaa taaattttat
    80041 tcatcaacac atcgaggtcc gtctgaactt tgcggaaatg ttttcaaaat tgaatataca
    80101 aaaaaaagac aacgtcttgc atatatacgc ctttatagtg gagtactaca tttacgagat
    80161 tcggttagag tatcagaaaa agaaaaaata aaagttacag aaatgtatac ttcaataaat
    80221 ggtgaattat gtaagattga tagagcttat tctggagaaa ttgttatttt gcaaaatgag
    80281 tttttgaagt taaatagtgt tcttggagat acaaaactat tgccacagag aaaaaagatt
    80341 gaaaatccgc accctctact acaaacaact gttgaaccga gtaaacctga acagagagaa
    80401 atgttgcttg atgccctttt ggaaatctca gatagtgatc cgcttctacg atatta
//
LOCUS       DACDIE010000006        78013 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.006, whole genome shotgun sequence.
ACCESSION   DACDIE010000006 DACDIE010000000
VERSION     DACDIE010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 78013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 78013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..78013
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.006"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(365..2000)
                     /locus_tag="IV250_000492"
                     /pseudo
     CDS             complement(365..2000)
                     /locus_tag="IV250_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2323..2802)
                     /gene="rlmH"
                     /locus_tag="IV250_000493"
     CDS             complement(2323..2802)
                     /gene="rlmH"
                     /locus_tag="IV250_000493"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAP8767802.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            3230..4519
                     /locus_tag="IV250_000494"
     CDS             3230..4519
                     /locus_tag="IV250_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP8767803.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(4558..5163)
                     /locus_tag="IV250_000495"
     CDS             complement(4558..5163)
                     /locus_tag="IV250_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAP8767804.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(5368..5832)
                     /locus_tag="IV250_000496"
     CDS             complement(5368..5832)
                     /locus_tag="IV250_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP8767805.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(5854..6174)
                     /locus_tag="IV250_000497"
     CDS             complement(5854..6174)
                     /locus_tag="IV250_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP8767806.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            complement(6318..7397)
                     /gene="pepA"
                     /locus_tag="IV250_000498"
     CDS             complement(6318..7397)
                     /gene="pepA"
                     /locus_tag="IV250_000498"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAP8767807.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            7597..7947
                     /locus_tag="IV250_000499"
     CDS             7597..7947
                     /locus_tag="IV250_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP8767808.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            8170..9117
                     /locus_tag="IV250_000500"
     CDS             8170..9117
                     /locus_tag="IV250_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP8767809.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(9174..10592)
                     /gene="pepV"
                     /locus_tag="IV250_000501"
     CDS             complement(9174..10592)
                     /gene="pepV"
                     /locus_tag="IV250_000501"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAP8767810.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            10741..11586
                     /locus_tag="IV250_000502"
     CDS             10741..11586
                     /locus_tag="IV250_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HAP8767811.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            11590..12030
                     /locus_tag="IV250_000503"
     CDS             11590..12030
                     /locus_tag="IV250_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAP8767812.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            12080..12274
                     /locus_tag="IV250_000504"
     CDS             12080..12274
                     /locus_tag="IV250_000504"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8767813.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            12410..13096
                     /locus_tag="IV250_000505"
     CDS             12410..13096
                     /locus_tag="IV250_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8767814.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(13504..14193)
                     /locus_tag="IV250_000506"
     CDS             complement(13504..14193)
                     /locus_tag="IV250_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8767815.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(14168..16297)
                     /locus_tag="IV250_000507"
     CDS             complement(14168..16297)
                     /locus_tag="IV250_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAP8767816.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            complement(16345..17682)
                     /locus_tag="IV250_000508"
     CDS             complement(16345..17682)
                     /locus_tag="IV250_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAP8767817.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            complement(17934..18293)
                     /gene="rplT"
                     /locus_tag="IV250_000509"
     CDS             complement(17934..18293)
                     /gene="rplT"
                     /locus_tag="IV250_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAP8767818.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(18346..18546)
                     /gene="rpmI"
                     /locus_tag="IV250_000510"
     CDS             complement(18346..18546)
                     /gene="rpmI"
                     /locus_tag="IV250_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAP8767819.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(18618..19142)
                     /gene="infC"
                     /locus_tag="IV250_000511"
     CDS             complement(18618..19142)
                     /gene="infC"
                     /locus_tag="IV250_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAP8767820.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            19462..20094
                     /locus_tag="IV250_000512"
     CDS             19462..20094
                     /locus_tag="IV250_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP8767821.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(20096..21001)
                     /locus_tag="IV250_000513"
     CDS             complement(20096..21001)
                     /locus_tag="IV250_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP8767822.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(21086..21739)
                     /locus_tag="IV250_000514"
     CDS             complement(21086..21739)
                     /locus_tag="IV250_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP8767823.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVRV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVAI"
     gene            22061..23005
                     /gene="mvk"
                     /locus_tag="IV250_000515"
     CDS             22061..23005
                     /gene="mvk"
                     /locus_tag="IV250_000515"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAP8767824.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            22998..23975
                     /gene="mvaD"
                     /locus_tag="IV250_000516"
     CDS             22998..23975
                     /gene="mvaD"
                     /locus_tag="IV250_000516"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAP8767825.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            23988..25073
                     /locus_tag="IV250_000517"
     CDS             23988..25073
                     /locus_tag="IV250_000517"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HAP8767826.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            25070..26134
                     /locus_tag="IV250_000518"
     CDS             25070..26134
                     /locus_tag="IV250_000518"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAP8767827.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(26383..27186)
                     /locus_tag="IV250_000519"
     CDS             complement(26383..27186)
                     /locus_tag="IV250_000519"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAP8767828.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(27206..28048)
                     /gene="kduI"
                     /locus_tag="IV250_000520"
     CDS             complement(27206..28048)
                     /gene="kduI"
                     /locus_tag="IV250_000520"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HAP8767829.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(28064..29071)
                     /locus_tag="IV250_000521"
     CDS             complement(28064..29071)
                     /locus_tag="IV250_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAP8767830.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(29078..29722)
                     /locus_tag="IV250_000522"
     CDS             complement(29078..29722)
                     /locus_tag="IV250_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAP8767831.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(29822..30580)
                     /locus_tag="IV250_000523"
     CDS             complement(29822..30580)
                     /locus_tag="IV250_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HAP8767832.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(30707..31858)
                     /gene="fucO"
                     /locus_tag="IV250_000524"
     CDS             complement(30707..31858)
                     /gene="fucO"
                     /locus_tag="IV250_000524"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HAP8767833.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(31940..32251)
                     /gene="rhaM"
                     /locus_tag="IV250_000525"
     CDS             complement(31940..32251)
                     /gene="rhaM"
                     /locus_tag="IV250_000525"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HAP8767834.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(32253..33098)
                     /gene="rhaD"
                     /locus_tag="IV250_000526"
     CDS             complement(32253..33098)
                     /gene="rhaD"
                     /locus_tag="IV250_000526"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HAP8767835.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(33112..34368)
                     /gene="rhaA"
                     /locus_tag="IV250_000527"
     CDS             complement(33112..34368)
                     /gene="rhaA"
                     /locus_tag="IV250_000527"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HAP8767836.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(34365..35825)
                     /gene="rhaB"
                     /locus_tag="IV250_000528"
     CDS             complement(34365..35825)
                     /gene="rhaB"
                     /locus_tag="IV250_000528"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HAP8767837.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            35978..36847
                     /locus_tag="IV250_000529"
     CDS             35978..36847
                     /locus_tag="IV250_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP8767838.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(36973..37872)
                     /locus_tag="IV250_000530"
     CDS             complement(36973..37872)
                     /locus_tag="IV250_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP8767839.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(38037..38861)
                     /locus_tag="IV250_000531"
     CDS             complement(38037..38861)
                     /locus_tag="IV250_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP8767840.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(38872..40167)
                     /locus_tag="IV250_000532"
     CDS             complement(38872..40167)
                     /locus_tag="IV250_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HAP8767841.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(40183..40662)
                     /locus_tag="IV250_000533"
     CDS             complement(40183..40662)
                     /locus_tag="IV250_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAP8767842.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(40668..41696)
                     /locus_tag="IV250_000534"
     CDS             complement(40668..41696)
                     /locus_tag="IV250_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HAP8767843.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(41713..42819)
                     /locus_tag="IV250_000535"
     CDS             complement(41713..42819)
                     /locus_tag="IV250_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP8767844.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(43048..45645)
                     /gene="adhE"
                     /locus_tag="IV250_000536"
                     /gene_synonym="adhC"
     CDS             complement(43048..45645)
                     /gene="adhE"
                     /locus_tag="IV250_000536"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAP8767845.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(45950..46330)
                     /gene="yajC"
                     /locus_tag="IV250_000537"
     CDS             complement(45950..46330)
                     /gene="yajC"
                     /locus_tag="IV250_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAP8767846.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(46427..47572)
                     /gene="tgt"
                     /locus_tag="IV250_000538"
     CDS             complement(46427..47572)
                     /gene="tgt"
                     /locus_tag="IV250_000538"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAP8767847.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            47790..48488
                     /locus_tag="IV250_000539"
     CDS             47790..48488
                     /locus_tag="IV250_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP8767848.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(48656..51295)
                     /locus_tag="IV250_000540"
     CDS             complement(48656..51295)
                     /locus_tag="IV250_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP8767849.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(51914..52945)
                     /gene="queA"
                     /locus_tag="IV250_000541"
     CDS             complement(51914..52945)
                     /gene="queA"
                     /locus_tag="IV250_000541"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAP8767850.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            53138..54313
                     /locus_tag="IV250_000542"
     CDS             53138..54313
                     /locus_tag="IV250_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP8767851.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            54317..54958
                     /locus_tag="IV250_000543"
     CDS             54317..54958
                     /locus_tag="IV250_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8767852.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            54955..55872
                     /locus_tag="IV250_000544"
     CDS             54955..55872
                     /locus_tag="IV250_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8767853.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            55869..56540
                     /locus_tag="IV250_000545"
     CDS             55869..56540
                     /locus_tag="IV250_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8767854.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(57081..57569)
                     /locus_tag="IV250_000546"
     CDS             complement(57081..57569)
                     /locus_tag="IV250_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HAP8767855.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(57770..58036)
                     /locus_tag="IV250_000547"
     CDS             complement(57770..58036)
                     /locus_tag="IV250_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP8767856.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(58036..58299)
                     /locus_tag="IV250_000548"
     CDS             complement(58036..58299)
                     /locus_tag="IV250_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP8767857.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            complement(58292..58564)
                     /locus_tag="IV250_000549"
     CDS             complement(58292..58564)
                     /locus_tag="IV250_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8767858.1"
                     /translation="MYWCCCIDSQALDSTFAKDDTLLPITLRLATASRKGVRSERGQR
                     SSLKRGLFLYVKKRKSLLNLSALLYFFDVLNQVRYNYIRKRRRDNG"
     gene            58775..58960
                     /locus_tag="IV250_000550"
     CDS             58775..58960
                     /locus_tag="IV250_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8767859.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(59102..60925)
                     /locus_tag="IV250_000551"
     CDS             complement(59102..60925)
                     /locus_tag="IV250_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAP8767860.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(61523..64228)
                     /locus_tag="IV250_000552"
     CDS             complement(61523..64228)
                     /locus_tag="IV250_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAP8767861.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(64534..65481)
                     /locus_tag="IV250_000553"
     CDS             complement(64534..65481)
                     /locus_tag="IV250_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HAP8767862.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLEASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            65671..65940
                     /gene="rpsN"
                     /locus_tag="IV250_000554"
     CDS             65671..65940
                     /gene="rpsN"
                     /locus_tag="IV250_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP8767863.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(66116..66478)
                     /locus_tag="IV250_000555"
     CDS             complement(66116..66478)
                     /locus_tag="IV250_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP8767864.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(66489..67610)
                     /locus_tag="IV250_000556"
     CDS             complement(66489..67610)
                     /locus_tag="IV250_000556"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAP8767865.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(67626..67976)
                     /locus_tag="IV250_000557"
     CDS             complement(67626..67976)
                     /locus_tag="IV250_000557"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAP8767866.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(68106..69617)
                     /locus_tag="IV250_000558"
     CDS             complement(68106..69617)
                     /locus_tag="IV250_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP8767867.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(69939..71291)
                     /locus_tag="IV250_000559"
     CDS             complement(69939..71291)
                     /locus_tag="IV250_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAP8767868.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(71444..72520)
                     /locus_tag="IV250_000560"
     CDS             complement(71444..72520)
                     /locus_tag="IV250_000560"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAP8767869.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            72716..74461
                     /locus_tag="IV250_000561"
     CDS             72716..74461
                     /locus_tag="IV250_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAP8767870.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(74741..75178)
                     /locus_tag="IV250_000562"
     CDS             complement(74741..75178)
                     /locus_tag="IV250_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAP8767871.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(75162..76535)
                     /locus_tag="IV250_000563"
     CDS             complement(75162..76535)
                     /locus_tag="IV250_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8767872.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(76857..77345)
                     /locus_tag="IV250_000564"
     CDS             complement(76857..77345)
                     /locus_tag="IV250_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8767873.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(77367..77969)
                     /locus_tag="IV250_000565"
     CDS             complement(77367..77969)
                     /locus_tag="IV250_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP8767874.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT        22479 a        16610 c        14233 g        24691 t
ORIGIN      
        1 tttctcagaa acaaaccact cattggaaaa gacatgaatc aaatcatcac ttacttgtgt
       61 aaaatagcga cgtttaatga caaagatatc ttcattgcta tctagtgtac cttcatttct
      121 ttgtgcttaa attcaaagat aattttctca ataaagattt ttgattccag taagattcct
      181 ttactccttt tacaacatag cgttgtcctt tgttttgaaa catcttcttc ccctctttgc
      241 cattaaaaat agcccaattc tctataaaga agattaagct tcattattat aatataaagt
      301 ttaaatttgg tttattactt actttttctt attctctagt aagctaatat tgaaaaaaat
      361 gtaatcagat cattattttt atatgttgta acttcctgat aagaaaacta attaaaagaa
      421 agaggtaact acataaacta attaaaaaag ctaacttcat agttggagga taaatggaag
      481 aaacttcaat gattgtttct ccttgtgttc cttcaatcga cactagtcct attatcaatc
      541 tctctttttt gtccatttac ttttgcgact gttccccaat aatcaaatac aggaagcgtt
      601 attttttgtc ctttagattc atgtactgta tagactattt tcgaatccat cacttgataa
      661 ggcatagtat gccattcctt gccaattctt acttcatgat tctgcaaatg ttctttgtat
      721 tgtaatgctt ccgtgggtgc atagtcataa agtcctccgg gaaggagtgt cgtgtaacga
      781 ataagatttt cttgattcat taccaagtga tctctctgag tgacaacttt atgttcatag
      841 tttgcttttg ttgcagcaga tacagccaac agtaataaaa caattgccgt tatattaact
      901 acttttcttt gcttagctga tgtgctcttc atgaaaacga acgataaaat caacgagcca
      961 aacagtactt gaaagcctaa gattcgccaa ggaaattgaa tcatttgaat gggtgtattt
     1021 tgtaataaag accatggaaa aaatttggtg gataagagcc aagagactac cgcactaagg
     1081 taaatcactt tttctttgcg attaaatcct ttaaaaaaga agagtggaca gatcaaagaa
     1141 ataataccaa tgattcctac cgtaaaggtt gtcaggtcat tattcatcgc accaatcaaa
     1201 ctatccgaga catttaatgc ttggtgttgt aaggtcgttt tatctggctc attaatcgat
     1261 tgaaccatga tttgtcggaa catcggatac caaaagtatg ctgttaaaag aatcgtcatt
     1321 cctactgcct ttgacaactc cacaatcaac cggatgcttt ctttttttct tgtttcactt
     1381 actacaaagc cgcctaagac tagtaagcca acggctatta cactcataaa agtggaaaga
     1441 acatgtgtat aaatgagtaa gctcatactg actgctaatg gaattgcaca tggctttttc
     1501 tcataaatca gtgcgtaagc ataatacata actatcggta taaccgtaag tgcaatcgct
     1561 tcagcaatag cagaacgatg ataaatatca atcgtacggt acccataaaa tgtatataaa
     1621 cagctaaata taaaagccgc atcgattctt tttaaaaatt ttagtccata atgaaacatc
     1681 agcatgatag tacaaacatt caatagccaa acatataaga tataactaac gattaaatta
     1741 ttacttatcc aataaaaaat gactgccgga aaaaaggtca agtagggata aaaataattc
     1801 actccgctcc cataatgatt aaacgtcgta aaattgattg gaccagaaaa aattgaagat
     1861 aaccctttga ctctagcaaa atggaaggca atatcccccg atgtagaaat agtatgaaca
     1921 tttctaaaat aattgattga ataaagtcca gcaataattg ctaggagaat ggacaatatt
     1981 atttttttct ttacttgcat tacttaatta tatactcctt tcatggattg ctttatctag
     2041 tataacttaa attttaaata aatcgttatt tatttttact ctcacttaaa aaagcactaa
     2101 aattataaga gagggaggta atggtctgaa caccaagaat tgttccaaca tcgaattgct
     2161 taactaatga gttcaaagct taattatgtg gctcttatat ttaccgagcg tggattgcga
     2221 tctctttagc gaaacaaaca tctttcataa aaaaaacagc catatccgct ttcttggata
     2281 tgactattta ctactacttt actatctttc attcttcttg tctcacttat gataaggcgc
     2341 ccccgcattg atccggaagg cacggtagat ttgctcgaca aggatcagac gcatcaattg
     2401 atgtggatac gtcatttttc caaaagagat ctgagcattg cttcttttca tgacggcttc
     2461 gctgagtcct aaagagccgc cgataacaaa aacaaggtgg ctttttcctt ggatacctaa
     2521 ttggtcgatt tgtttagcaa aagattcact tgtaggattt ttcccttcaa ttgctaaggc
     2581 atagacgtat tcttgttctt tgattttcgc taagatccgt tccccttctt tttcttttac
     2641 ttgtctcatt tgcgcttcgc ttaaattctc cggggctttt tcgtcaggaa cttcgatcaa
     2701 ttcgattttt gcataggcat tcagccgttt gacatactca ttgatacctt ggatcaggta
     2761 tttttctttt aattttccta ctgagataat tttgatattc atgttttctc tcctttggtc
     2821 ttcatgctca ctttgtttat tataatcaat tttttcgtct atatgtttat tttggctatt
     2881 tttgttaaaa agatttcccc aacagttatg cacaacatcc acaggtttat ccacagaaaa
     2941 cagtgttatt ttaccttatt tcatcacatt cattaggcaa acatacgttt tataagaatt
     3001 tatccacttt atacacaggc tgtggatatg ttttatccct tatttttcca atcctatccg
     3061 cgtagtttta cacagtatct gtggaaaaag aaaatcaaat attttcatct gaaaacaatg
     3121 atttttccta aagtttttca taaaaagttc acagtctcaa agtagactaa agtcaacaaa
     3181 taagactata ctaacagtag ataaatgaat gtaattggag gaggaacaca tggatagaaa
     3241 aaatgtgaca ccaaagatga aaaaaagtaa aaataatagc ctctggcgta agttaggtct
     3301 ggggcttgtt ggagggatcg ttggaggact actgacagct ggcatttttt atgcagcgat
     3361 gggcactggc aacaatagtt ctactgctac aagcggcaat cagaattctg ccggtgaaac
     3421 agttgtggaa aatgtcaaag tcaatgtaga ttctgatatc accaaagcgg tagataaagt
     3481 tcaagacgct gtcgtttctg ttatcaactt acaaagtcaa aaccaatcaa gcggttttgg
     3541 cggactattc gggcagcaag aagaaagtag cagctcagat gatagtaatt tggaagctta
     3601 tagtgaagga agcggcgtga tctacaaaaa agacgggaat actgcttatg tagtcacgaa
     3661 taatcacgta gtagatggcc agcaaggtct tgaagtcttg atgaaagacg gtacgaaggt
     3721 aaaagctgaa ttagttggaa cagatgctta ttcagattta gcggtgttga agatcaattc
     3781 agacaaagtc gaaacagttg cctcttttgg tgactcaagt gctctgaagg ttggtgagcc
     3841 tgcgattgcg attggttccc cactaggatc tgaatatgca aattctgtca cttcaggaat
     3901 catttcttcc ttgaaccgac aagtgacaag tacgaatgaa tcgaacgaaa cagtcaatat
     3961 caatgcgatc caaacagatg cagcaatcaa ccctggaaat tctggtggtc ctttagttaa
     4021 tatcgaagga caagttatcg gtatcaactc aagtaagatc gcaagtactt ctgaatctac
     4081 ttctaatgta agcgtagaag gaatgggatt tgctattcca agtaacgatg tagtgaatat
     4141 catcaatcaa ctggaaaaag acggaaaagt gacacgtcca gctctaggta tcacaatggt
     4201 cgacttatct gccgtttcta ctcagcaaca agaacaaatc ttgaagattc cttcatctgt
     4261 aactaacggt gtcatcgtga caaatgtcca aacagctaca ccggctgaaa aagctggatt
     4321 gaaacaatac gacgtcatta ctaagatcga tgacaaagaa gtttcttctg gtgtagaact
     4381 tcaatctgct ttgtataaga aaaaagtggg agattctgtc aaaatcactt actatgaagg
     4441 ctcgaaagaa aaaacaacta ctgttgaatt gacgatcgat caatcggcat taaaacaaaa
     4501 tcaaaatagt gaaaattaat aaaaaagagg ttgtgacaaa gacttgtcac aacctcttta
     4561 ttttggaaat gcttctccga ctacggcatc ctcaggcaat actaagattc ctttttttgc
     4621 tggagcattt ggcaaatgca attcacgagc ggaacagatc attccatagc tatctacccc
     4681 tcgcaaagtt cctggccaga tcatcattcc atctggcatc atagctcctg gttttgcaac
     4741 gacaactttt tgtccagctt cgatattggg tgcaccgcaa acgatttgca atactgtacc
     4801 attgtctact tctgtctcag taatcgataa atggtcagaa tcaggatgtg ggacgcatgt
     4861 ttttacgtac ccgacaacga tcttcggttc atgatcagct tcaagctgtt cttcgaatcc
     4921 tgcttctttg atcaatcgat tcaatttcgc taattcctca tctgttaagc ttacttggcc
     4981 gttcccttcg attgttagat gatcggagat atggaaaaag ttccaagcaa caacttgacc
     5041 ttgttcatct ttgatccgag caacattcca tttgcgttca acagtgtttt ctgttccttt
     5101 atcgtcggct ataataacca tcaaggtgtc tccgacgtgt gctttgttat aagcaaaaat
     5161 catgttcttt cctccattat cctttgctga gatttatctt aacaaaaaat tgcattcact
     5221 gcatgaaatt tttttcatgc tttatttccg aagaagcctc ttctggaaca ccatttattc
     5281 gtaacaaggg actgtgacaa gacttttgtc acagtccctt tactgtttta gttaattgat
     5341 taaaaaggtc gtacaagttc cacttgctta attacttggc tgttcttctt cgtcagaaac
     5401 gatcaagaca tcacaaggtg aatgacgtat cacgtagctg gctacacttc ctgtcataaa
     5461 gcgttcgact gcatttagac ctgactgccc aaccatgatt agatcgatct catatttttt
     5521 tggcaatttg actgccatgg attcacgaac agaaccatat gcaataattc cttctatatt
     5581 tgtgtaatca acagatttcc catattcttt acattcttcg atcatttcct tggctgtttc
     5641 agtttgctga tcgactagat tttgatcaat aggtgcatag cccataaagt tataaaattg
     5701 ctgatcgatt acgtttgcta catatacttt cccattatta cggcgagcca cttctaccgc
     5761 ttttttgaaa gcagcaagtg cttgttcact accgtcgata cctactaaaa tattcttata
     5821 ttcttgattc atcttgaccc ctcctaacag atgtcagcct tttgttacac tttctaggaa
     5881 cgattcgatt tcctgttttg ttttacgatc tttattcact agacgaccta attcttttcc
     5941 gtcttggatc acgacaaaac ttgggatacc aaaaatgttc cattcagcgg ctacatctat
     6001 gtacttgtca cggtctactt caataaattg ccaatcagaa aagtcatttt caatttcagg
     6061 catttgcggt ttgataaaac ggcagtctcc gcaccagccg gcagtaaaga aaaatacact
     6121 ctttccagtt tctacgtagc ctgctaattc ttctaaacta tttgggataa tcattctttg
     6181 tcctctactt tctgtttatg tatttttcaa ttaaattata cacctttctt tcgaactaac
     6241 aaacattgat cctcttttta taaaaaaaat ctggcaaaaa gcaatctttt gccagatttt
     6301 atagtttcac tatcgcatta ttttccgtat acgatcgtat ttactgttga tttatccaaa
     6361 cctttcagtg tttgaatcaa catttctcgt gcagcttcaa aatcgcgaat actgaacatc
     6421 gtttgatgcg tatggatata acgtcccact acgccaatca ctgtacttgg gattccttcg
     6481 ttttgtgtat gagcagcacc tgcatctgta ccgccttttg aaacaaagta ttgataaggg
     6541 atattgtttg tttcagcgat atccagcaag tactcgcgca aacgaggaag catgatcaaa
     6601 cctggatctt ggatacgcat caatgtacct tctcctaaat gtccgaatgt gccatttttc
     6661 gtcactgtat catctgctgc agagcagtct actgcaaaga acagatctgg cttgaattta
     6721 ttgacagaag ctttagaacc acgcaagcct acttcttcct ggacattcgc gcctgcaatc
     6781 aatgtatgac ctaatgtttc attttgcaac gcttctaaag cttctaatac cattgtacag
     6841 ccatagcggt tgtcccatga tttactgata atattctttc cgttggctgt tttgatcgtt
     6901 tcagcgtaag gaacgattgt gtctcctgga aggacaccgt aactcattgc ctcttctctt
     6961 gattcaaacc cagcatcgaa caaaatatcg gttacttcta ctgatttttg tccgcttgtt
     7021 cctcgcaata aatgtggggg aacggaagaa gaaatacatg gataattctt accagtagag
     7081 gttttaagtg taaaacgctg agcagaaaca acgtaaggat tccagccgcc tagaggtacg
     7141 actttaaaaa gcccgttttc ttggatttgt gtcagcatga atccgacttc atccatatgt
     7201 gctgcgacca ttacacgggg tgcatccgct tcttcatgat ggcgcagacc aaaaatgccg
     7261 cccaaaccat ctaactgtaa ctcatctacc agcggttcca tatgactttc catataagcg
     7321 cgaacatctt gttcaaaacc gcttgttcct tgtaattctg ttaactcttt gattcgttga
     7381 aatgtttttt cttccatatg ctattcgtct aaatttcaga ctaaactcct ctcagactta
     7441 tattgatctg cggataaaat atgatttttt ctgcagaccg atcgtattta ttatagctta
     7501 ttttttctgg tagcggttat ttatttcaaa gaaaatatga gagtgtggta aaataagcac
     7561 aaggagtaac aagtgagaag aaggaggtat gtccacatgt atgaagaaag cgaactaagc
     7621 tattttaaag gcgggttagc agttggaatc ggtctaggat tattgagcgg ggtagcttca
     7681 acgatttggt atcatcgcaa aaaagtattg tcagcagacc aagtacttga aaatatcaag
     7741 tcatcttttc taaaagaagg tgcaatcgaa ggttcttgga tctcttttga aaaggaacct
     7801 attaggaaat tcgccgttcg gtcgagtgct tatcggggag gtctttctcg aatagaagat
     7861 ggcgaaactg tttattatga atttttagca gatgctcata caggaaccgt attagaaatt
     7921 tcaagaaaaa aaggtgcaac agattaatac gtcaaaaaag tgagagaacg aatacgcacc
     7981 cactattcaa aagagaggta tcatgtcctt cttttgtagt gggtggtttt ttagatagag
     8041 ctttccaaac atatattcca gtattttctt tttaaataaa tgatatttag cgggaaaaac
     8101 tagcatttcc tttgattatg aggtattttt aatttagctt ttgttttcta tagttaagga
     8161 gagtgaaaaa tggaacaaca agaaaaaatt gctgttctga ttccttgtta caatgaggaa
     8221 gcaacaattt caacagtcat tgcagacttt aaacgagaat taccagaagc agatatttat
     8281 gtctatgata ataattcgac cgatcgaaca tacgaattgg ccgttcaagg tggagcaatc
     8341 gtaaaaaaag aacctcgtca aggaaaagga aatgtgatcc gccaaatgtt ctttgatatc
     8401 gatgccgact attatttaat ggtagatggc gatgatactt atcctgcaga agctgtacat
     8461 ggtttgctcg agaaattacg ttctggtgaa gcagatatgg tcattggtga tcgcctatca
     8521 aatggaacct actttgatga gaataagaga cctttccacg attttggaaa taaccttgtt
     8581 agaaatacaa ttactagaat gtataaaaca aaaattcttg atgtcatgac cggctatcga
     8641 ggattcaatc gtatctttgt caaaagtttt cctattatga gttctggttt tcaaatcgaa
     8701 acggaattga cgatccatgc tttagataaa aaattcaagt tcgttgaaat cccaatcgat
     8761 tatcgtgatc gtcctgaagg cagcgaatca aaattgaata cattctctga cggattcaaa
     8821 gtgatcatga tgattattaa aatgtttaaa gattataagc ctttgctttt ctttgggatt
     8881 tggaccattt tcttcttttt atttggattg gtcacgggga ttcctgtgat tcgcgaatat
     8941 atgctgacaa gctttattac acgcatccct tctgcgattt taagtactgg attgatgact
     9001 ttagcacttc tttcattagt aacagggttg attttggata cagtcgtcac gaacgctaag
     9061 aaagaatatg aattgaatct atatcatgtt tatcatgaat accacagtag aaagtaaaaa
     9121 aaggatcaag actgcaaaaa taatttgcaa ttcttgatcc tctttttaat taattatttc
     9181 gccaaacgat agatggcatc tgcatagatt gctgttgctc tgaacaagtc atcaagactc
     9241 ataaattcgt ttgcttgatg cattgtatca gtatatcctg ggaacatcgc tccataagca
     9301 acgccacgtt tcaacaaacg tccgtaagtt ccgccaccga tgatttgttc gtagcctttt
     9361 tctccagtgt ggtcttcata gacttgcaat aatgtagcca ccaatggatc atccattggt
     9421 acgtaatgtg gttccattac gcgagcacct cgagtaactg ttgcgccttc ttgccccact
     9481 gtttgttcga tgtcactttg caatccgtct actgttacac cttttggaaa acggaaattc
     9541 atattgatga agttcgcttc ctctgtacca ttttcttcaa aagcaaatag tccagcattc
     9601 attgtcaatt tgcccatttt ttcatcttca taagcgactc ctagtttttc tccataaaaa
     9661 tcttcatgga cataagcagc tgctacatgg ataaattgtt tagcagatcc taggaattca
     9721 tagttatcta agaacaatgc caagaaagaa cctgcattga taccagattg tgggcttgca
     9781 ccatgtgcgc ctttaccaac taattcgatt tttacatacg tattgtccgc ttcaatcgtt
     9841 ccgcttactg gatttgcttc gatgaattgg taaaatgctt cttccatcgc gatagctgca
     9901 tctgcacttg gtacttgaag tgctgcagtt gctgttcctg gcaccatatt ttcgcgaagt
     9961 ccggaaacaa aagatttcaa gacataatct cctgcattgt cgcctttgaa gcgtaaagct
    10021 aaagaaacat ttcctttttc accattgatg attgggaatt cagcatctgg tgagaaacca
    10081 aaatctggtt cttcttcatg ttcgaaataa taagccatat cgccccagcc gctttcttca
    10141 tcgctgccga ctacgaagcg aacttttttt gatagtttca agtcaagttc tttaatgatc
    10201 ttcatcgcat agtaagctgc catacttgga cctttgtcat cgcttgatcc gcgtgcaaag
    10261 atctttccat ccttgattac tggttcataa ggatctgtgt cccagccatc tcctgcagga
    10321 acgacatcca tgtgtccaaa tatccctagc gtttcgtctc cttcgccaag atcaatatgc
    10381 cctgcatagt tatcgacatt ttttacttta aatccatcac gttcgccata agctaacata
    10441 tgtttcaatg cgtcgcgagg tccaggtcca aatggtgcat ctggtgttac ttttgagtca
    10501 tcgcgctcgc tgttgacacg taaaagattt ttcaagtctt caagcaagtc ttctttacgt
    10561 gcttctactt ctttttgcca atcaattgtc ataaagttaa tcctccactt atttaagtct
    10621 tcgtgttcat gataccacaa attacagaaa aaaatagttc aaaaaatctg acaattcctt
    10681 ttcttatctg tttttctcta aaaaaaatat acgctataat gagtaaaagg aggtttttag
    10741 atgctgataa acgaacaact agtgaaaaga gtcatcacac ctcgtccaaa aatttcacat
    10801 aaaggaacat ttggacgtat cgttttgatt ggcggcaatg aacaatatgg cggagcaatc
    10861 attatgagtg ctttagctgc cgtacacagc ggagcgggtc tcacgactgt agtaactgat
    10921 gaaaaaaatc ggagtgctct tcacagtcat ctgccagaag caatgattgt agtttggacg
    10981 gatactgacc agtttctttc cgtaataaag caagcagatg tactcttgat tggtcccgga
    11041 ttgggtactt cttctaagag tttggaacgt ttcaaacaag tactagaaaa ccaatcaaaa
    11101 gaacaatggc tggtgattga tggctctgct ttaagtttac ttgctgctaa tcatcttttg
    11161 ctaccctatc ccgaacaaac tgtacttaca cctcatcaaa tggagtggca acggttgagt
    11221 tctctaccga tacaagaaca acgggataaa gaaaacaaaa agaagcaaca ggaaatcggt
    11281 gctgtgctcg ttttgaaaag tcatgagaca aaaatttatg gtcaccagca cttctacacc
    11341 aatcctttag gttctgctgc tatggcaacg ggcggcatgg gtgacacttt agctgggatg
    11401 atcacaggat ttttagccca gtttcctcaa aatgaccaaa cgattgcagc agctatttat
    11461 cttcatagtt tcattggaga ccagctggca aaagaacaat atattgtttt gccgacacaa
    11521 atcagcgaag cacttccgaa atggatgaat tatttttgta aaaaatcaat ggaaaacgag
    11581 gcatgagtca tggagaaatt tacaggttat gtgcgagaac ctttttatta tgaaacagat
    11641 caaatgggga ttattcatca cagcaattat atccgctggt ttgaagaggc tcgggtagcc
    11701 ttattggtac atctagggtt tccctataaa aaaatcgaag aatctgggat catcatcccc
    11761 gttctggaag taaattgcag ctataaagaa atgatccatt acggagaaaa agtgagcatc
    11821 catccaacta tccaaaagta tacggggaca cggttggatt ttagctacga aattgtggga
    11881 actgaagaca aaaaattaaa aactactggc agtagtaaac actgtttttt actttctgat
    11941 tcgcaacgat tagtgaagct gagcaaagtc aatccggagt tagatcagtt attccaagcg
    12001 tacgcttctt tggatgatgt aaattcttaa taaagaccgt ctaagcattt ttcttgtatt
    12061 gtgtcgagta gttcgtataa tgaaaagaaa aaagcaggtg atccccatgt atgtcgttaa
    12121 agtaatgcac ggttacatcg ataaaactgg ctgtcgcact cgggaaaaaa acttagacaa
    12181 tctgctcatt ttcaaagaca aaaaagaatc cgaagctttt gctaaaagaa tcggcggtcg
    12241 tgtcaaaccg atccaagagg tacgaccaga ttgattcaat atttttcttt tcgaggctct
    12301 aataggcatg cctatcaggg ctttttttgc agactgtgtt tttatgccca cacgatttgt
    12361 aagcgattta tgttataata gattatatct gcatagaaag gaaggttaaa tggttatcaa
    12421 taatttgcaa cagcaagctt atgaatctat acgtaaacaa atcatttatt ccgagttagc
    12481 acctggaagt aaaatctccg ataaagtgtt agaagaacaa ctagctatcg ggcgtacacc
    12541 gattcgagaa gcactgatcc aactgcgcaa tcaagagctt gtaaaaacca ttccacagtc
    12601 cggcacttat gtttcaaaaa tcgatatccg atctgcttca ttagcccgtt atacgcggga
    12661 aaagttggaa aaaccgatcc tgcaagaatg cagtgcgaaa atgaccaagc aagatcaagc
    12721 taaactagaa aaaatactag aacaaaccga tcaggcagta atcgctcaaa ataaaaaagc
    12781 tttttttgat ttggatactg attttcaccg aacttgttat gaaattgctg gaaaacgaga
    12841 aatttgggat tggctagaaa gttatagtac gcacttgaac cgtttcagat ggttacgttt
    12901 gacgatttca gaattagact ggggtcgagt attagatgaa catcagacaa tgcttcaatc
    12961 aatgattgat cataactttg atgaagtcgg atttctttgc acgatgcatt tacatatgat
    13021 tatcgaggag caagaatacg tgattcataa ttaccccgat tattttgaag atattcagga
    13081 cagacccatc aaataagagg ttttggaatc agcgttttga cctgagtatt agagaaagaa
    13141 acggcaaaat agttcctcat attttgccgt tttttttgag ctaataccga gggtcagctg
    13201 attgcacacc gtttattgag aggttgtgaa aaagctgccc tgcatcaagt aataagaaca
    13261 gccaagtaaa aatagcttct tatgttttta acttggctgg acttattacc gcagatgcaa
    13321 gcttttgaac accgttttcc aagaggtcgt gagcctgccg ttcatctccg agtcacaagg
    13381 aacaatttca aaaaacagct tctcatgttt gttgaatatt ttgacttgtg accgaggagt
    13441 tgacaggaga acatcgttta ttcggaaaaa aaggactgtg acacgacttt tgtcacagtc
    13501 tctttatttg taatctaaat catactccgt acatcttttt tctatttttt ttatttcttc
    13561 taagatccct tggctacgaa tttcattata tggccaaagt aagactgcct ctaatacata
    13621 aggcgaaaaa tcgctttgat tcatctcttt tgaaaaactc atcgctgcta tctcgctcaa
    13681 tgcacgaatg tttgattgat gtgtataaaa aggcaaccgc cacagcacaa ttttttcgct
    13741 ttgcgtataa attcccttaa cagaagcttc gatgtggtga aatagttcat gtccaataat
    13801 caaagcttgc gctgtttctt tagagatcga agctagtcct gaacatttta atttttctaa
    13861 cggctccgtc atcaatcgaa tcccttcatc ttctgcataa gttgcaaaag tgattcgttc
    13921 gtttatttgc tctgattcac aggaaacgat tggaacagcc atttgttcac aaatttctcc
    13981 tacatcggca aaagggtact tttttttgat gctacatgcc gtttcgatac cacaatcgat
    14041 tgattgttgt ataagaattt tctgcttgga caaaggaatc tttttccaaa ataaatctcg
    14101 ccgaaactgg tagaatcccc aaatttcttc ggggatctgt tgtaattcgt ttagtaactc
    14161 ttctaactta tgtttgtttg atatttccgg caacgacgat cacttcttcc tccggttcca
    14221 tagtagatac ttccagatcc atcaaaagct gtagttgttc cagatccgtc gctgtaaaat
    14281 cagagactct tgctcctaag cagagataaa attctggtcg ggcaatcaat tcagtttgat
    14341 aaactgccat atcttcccaa agttgttcga cagctgtcat ataattggct gcagttgttt
    14401 tcatacatga agcatgtcca cgttggacct tgataaatcc tttttgatcg atgatccgaa
    14461 gacttccagc attttttgaa cgattctgtt cgccgcatac ataaaagaaa ggtgtatttt
    14521 ccaataagca aacttcattt ttatttaatc gcatatcctc tgcagcaagc tctagtgctt
    14581 cttctgttgt gatctccttg gttagatcgg ttgctttcac ttccgtagaa ccagtagcga
    14641 tagccgttac ttttgaagtc tgtggatcga tttctacgtg gacttcgatc gattctggtg
    14701 tagcgcctga ttcaatcgct ttattcatcg cttcattttt caacgatcgt atatcttctt
    14761 tacttggcga agggatgata cgttctacaa catctcggac cattgcaagt gctacaccaa
    14821 tagaagaaat cacttcggcg ttttcaggaa tcgaatattt cacacccatt ttattactaa
    14881 agtaagtaat caatgaggca gcgccgcccc ctacacctac aagagaaatc tgatctttct
    14941 ccaagtgata cttgtctgcc aattctaaaa tcaccggttc aatcttagca taggattttt
    15001 ccatgatctg ttctgcgatg tcttcaaccg tcgtatgaca ataatcagct aatgcttgga
    15061 tggcttttcg tgcagatggg acatttccat aagaaaaatg ttcttcttgc actaatccaa
    15121 ggacattggc tgcacatgta tttgtaatag tcacttcttc tccgtcttcc atcactacct
    15181 tgacgtaatc agcagggtct ccttctttag gagagaaaaa ttcaacctta ggacctttga
    15241 ttttttctgt ctccgtaaat actgcataat ctagaccggc aatatgtgca gaacgtggac
    15301 cgacatcgat gattcctgac tgattagccc gtaccatcga accgcctgcc acacctagaa
    15361 cccgaacatc taatgaagag atatatgttg ggtggccacc tacaattgaa taatcgattg
    15421 ctggacgtcc atttttaatg accccgatat tggttgttgt tccgcctact tcaaaataaa
    15481 caccattaga cgctctcaag tacatcaaag agcccatgac tgatgctgct ggaccggaaa
    15541 gcatggttaa aacaggtcgt tttttcattt cattgatttc cattactccg ccgtctccgc
    15601 gcataatcat caaagaaaca ttgacccctg cttctcgtac ggaatcttct gtagaggttg
    15661 ccgtatctag catttttggt aaaatagatg cattgattgc tgctgtccga gttcttcttg
    15721 tcaatccata taattttgtg atgtcagaag ccatcgttgt agggatcgat tttaccgatg
    15781 ctgcttcata aacaactcgt tctggttccc cattatctac tccaaatgcc atagaagaaa
    15841 ccaagacctc cgctctttct ctttctaaag aagaaatcgt ttctgaaaca cggtctacat
    15901 ttaagtgttt gacaggtaag aatgcattga caatctctat ttttttctta ttccctaaat
    15961 cgatatcgtt caatctagtt tgtcgttttg ctaaaaaacc ttctaatcca ccttttgcca
    16021 tcccgattac gccgactttt gctacgtctc cttcaatcag tgcattagtc gcttgtgtgg
    16081 tagaatgtgc aacaaacaca acatcttctg gactgatatt attttccagc aaacaatttt
    16141 gaaaagactg aacgacacct gcggctactc cacggacatc atcgtgggtt gttttcactg
    16201 aagattttcc gatgatttcg tgcgtcgcat tatcgattgc gactgctttc gtatgtgtac
    16261 cgccaacatc aattcccatt cgcacaaaac gtttcatttt cttttcctcc attcatctaa
    16321 tcgtttttat cgacgttttg tttactaggc gttcccgtac aataagaacg tgatcaggca
    16381 taagatcgct ccgatgatcc aaccagttgg gatggatttc ttcatatatt ctcgagaagt
    16441 tacttttgta taacctaatc cccaagcaac ccaagattgt gtaatatcta aatgctgagg
    16501 agcaatcgtt gtgattgcaa ataacgggaa caaaaaggct ggcgacatcg ttggattaac
    16561 agctaatacg acagctaaaa tagctgagcc tgaaccgact agattcattg gaccgcggaa
    16621 aaatcctagt ggtgtcaata tagcaaatac gattgccaag actaatggtg attgcggtat
    16681 ccaatctcca aaaatagctg aaaaataagg agaagcatac gcagctgaat tattgaacat
    16741 cgctaaggtc atcaagaaac caaccattgg cgcaacatct actgcgccat ctgtaaataa
    16801 tttcgcaaac tgacgacaga tttctttata acctccgcgc aattttccag taaacagcaa
    16861 tgcccagata cctgctagaa taaaacctaa gatgattgga agatctaaaa gaacgacccc
    16921 taaaactggt aataatacgg ctaaccatga gatcattggt gcgtcgccac cttcagctgg
    16981 tacgtttgct gccattgcat agcgtttctt tttgttcatt gatatattag cgaccgttag
    17041 taccacaact aagcttacaa tcatcccaat cataccaatt tggaaatagt cattataagt
    17101 atagctttca gccgctggat tgaaaccagc ataaatcgtt tgatattgtt tgaaattgac
    17161 gatattagca aagattccag ccataatcga gcccataaat gagaacagtg caaccggtac
    17221 tgaaatacct aatgacatca agattggcaa cacaataact gcaatagaaa tcactggtcc
    17281 gattcctgtc atcgacatga atataacagc ggttacaata catagtaagc tcatcgtgat
    17341 tcttggcttg tcccctccaa gttcaactac ctttcgaatc agggtagctg cgattccgct
    17401 atctactaga acacgcccga aaaaggcacc aaagaagaca tttaccaaga tcgatttggc
    17461 gtactcagct ggccccgtcg tatagatatg cgtcaacaca tcaattactc cttgcccttc
    17521 catcgccggg tttggtgaaa acgtgtttcc gactagcgct aatcccatcc atacagtagc
    17581 catgatggca aagccgacca tcaagttgta gccttttaca cagtaccaga ccatgaaaaa
    17641 gaatgagata actaacaaaa taccaataat aatatcactc atgttattcc tccgtccatt
    17701 ttgtttttct gataagcaaa ctaatatatc agtcaaactc attctaactt atgtagttag
    17761 cgctttcatt aaagggtgac cataaatgtt cagttttcaa ttaaaaaacc ttaattatgc
    17821 tattttagac ttatacgtta ttgtcctgtt tcatttgatg gaggattgta aataaaaaaa
    17881 gaggaaagaa cataactttt cgttatcttc tttcctcttt ataattgttt agcttattta
    17941 gccaaagctt cttttgcttg gtcagccaat gcagtaaatg ctgctgcatc gttaacagcc
    18001 aagtcagcta acattttacg gttgatgtcg atttcagcaa ctttcaaacc gtgcattaat
    18061 tttgagtagc tcaagccatt catacgagct gctgcgttga tacgagcaat ccacaattta
    18121 cggaagtcgc gtttcttttg acgacgatcg cgatatgcgt agttgtatga attcattact
    18181 tgttcttttg ctgttttaaa taaagtgtgt tttgaaccgt agtaaccttt tgctaatttt
    18241 agcactcttt tacgacgttt acgagttact gttccacctt taacacgtgc catgtataat
    18301 tcctcctaat aaaaataaat cttcagattc tgttatttgt acttcttatt tcatttttgc
    18361 tagttgttgg cgaatacgtt tgaagtcgcc actagaaacc atgcttgctt tacgcaattg
    18421 acggcgttgt ttttttgttt tgccgtggaa acggtgactt gtaaacgcgc ggaagcgttt
    18481 caatccgcct ttaccagtac gtttgaaacg ttttgctgat ccgcggtgtg ttttttgttt
    18541 tggcatgact atatttcctc ctcaaaatct ttcgttatcc gactatcggc tgtccaacag
    18601 ccagttagtc attcaaatta cttactgtcg tttttcggtg ccagcgtcaa gaacatgctg
    18661 cgtccgtcca ttttcgcttt ttgttccact gttgcaatat ccgcagtttc ttcagctaag
    18721 cgattcaaga ctttttgacc aatttcttta tgggtaatgg cacggccttt gaaacggata
    18781 gaagctttca ctttatcgcc cttttccaag aacttacgtg cattacgaag ttttgtattg
    18841 aagtcattca agtcgatcgt aggacttaag cgaacttctt ttacattgat cactttttgt
    18901 tttttacgcg cttcgcggtc tttcttttgt tgttcgaaac ggtatttacc gtagtccatg
    18961 attcgcgcaa ctggtggttt cgcaccaggg gcaactaaca caagatccaa gtttgcttgt
    19021 tctgcaatat tcaatgcttc tgcttttgtc ttaactccta attgctcacc atctgaaccg
    19081 atcaatcgta actctcgtgc acgaatgccg tcgttaacca tcatatcctt tgctatggtc
    19141 attcacctcc aaatattatg agagaaagaa aagcttaccg aataaacggc aataaaaaaa
    19201 cgagttctct tacagaaccc gcgcgtactt cacctaatag aaccagtgaa aactatctag
    19261 cccagtgaca ttatcaacta aggcgagaag cgggtgcttc tgcttttatt tctcaaccat
    19321 catagtatag caaaaactaa tctttttgtc aatgtttttc atacagtttg tcttcctttt
    19381 ctgaaaatga ttagattagc agactttcta gcagtgattc ccgcttcttt gctatactaa
    19441 agaaaaacga ggtgaaccca catgaaaaaa atcgaaccta ttccaccttc tcttcctctt
    19501 cttacaaagg gagagtttta tttagaagat gaggagatct tctctggtct tctgatcaac
    19561 gggggtgacc aaagctatct cgatgccaaa aatatggtat taagagagag tcatatccaa
    19621 aaacttacga tgcaaaaaac agttttacgt cattttgaat gcagtaatgt catttttgaa
    19681 aaatgtgatt tttccaatct ggagtggatt ggtggcagtt tccaccaagt cgtgtttcat
    19741 caatgtaaat taacaggtac gaattttgcc gaaagttatc ttcgagactg tactttcact
    19801 gattgtattg cttcgatggc ttcctttagt agtacaaact tgaaagctgt ctccttcgat
    19861 cattgtcaat tagaagacag cgaattttat gaggtcactt ggaaaaacct ttttttgtct
    19921 gacaatcagt tgaccggttc gaactggttt cgaacttctc tgaagggatt agactttacg
    19981 actaatttct tcacagcaat cgctttatct caagattctt tggcgggact aatcgtcaac
    20041 caagagcaag cacttgtgat cgcccaagcc cttggtttgc agatcaaaga ctagatcaac
    20101 gaatccgccg ccacagtgca ataagcatcc aaatagataa gaccaaactg atgatcaagg
    20161 cgattcccca tccgaaaacg gaatgggtaa agggtaactg gagattcatt ccgaagaatc
    20221 ctgtcacaat agttggaacg gtcaagatca gcgaccagac cgtcaaaaat ttcatcgtgt
    20281 cattcaagtt gttattcagc aaattattat aagtgccaga gagctggtct aaaatctgcg
    20341 aagctaagtt cgtcatttca actgcttgtc gtgcttcgat cagcgcatcg tccaactctt
    20401 ctttttcctt ttctgacaac ttgcgataaa tagccaatgc cttgatttgt tctaaaagta
    20461 ctacattttg ttttgttcct gtaactaagt aaaccaaacc gatctctaaa tctgaaagtt
    20521 gcagcagatt cttgttcgtt gttttttctc ttaatttcag attaagccgc tgacgttcac
    20581 tattcacttc ctcgattaaa ggaaaaaagg aatcagaaat caaaaacaat gtacgaaaca
    20641 ataaactgta tacactcatg tctggcattc gttccaaaag cgatcgaatt agtcgaatca
    20701 cgtactcggt attcggcgtg gtaaatgtaa acaaaccgtc ttcttttaaa ataaaagcga
    20761 taggactagt ttcataatga ttatcgactt tttgctggtg cggaacattt gatacaagca
    20821 gtaacgcttc atcaaaagaa tcgtactcta cgcgcgcccg ttcattttta tctagagaat
    20881 aagtcagcat ctcgtcacta atgccatatt cttcttttaa gtttttaaca gctagcacat
    20941 cgttttgctg tacattgatc caaaaattac ggtcttgatt aaaatagtgt ttttcccgca
    21001 tactctatcc tcctgacatt tgtttccagt ataaagaaaa aagtcgctca aataaaggtt
    21061 taccctttat ttgagcaact gtaaatcaaa tggctacttt ggtttggcga atctctgcta
    21121 atttttcttc gatcattttc aagacttctt cttttgcttg aggatcttcc acaaaattat
    21181 agcggtctcc atcgatttgg atttttggac tttctttgta ttcttcatac cattgatcgt
    21241 aacgtctgtt caattctttg taataatcgt atagacttgg atcaaaggac aattgttcat
    21301 acgaacgacc acgtttttcg atacgctcca acatcgtatc aaaagataca cgaatatgaa
    21361 ctaataaatc cggatgtttt ttatgggcag catagggaag ttcttccatc atgttttcca
    21421 gcagttcatc atacacacga acttctgttt ctgttgctct tcctagatct gcatttagat
    21481 ggaaaagcag tgaatcttca taaatcgaac ggtccaatac attattgtca tctttcattg
    21541 cttgtttgat gcttgcaaaa cgtttattca aaaaataaat ttgcagcaaa aaagcatact
    21601 gttcagggtt tgcataaaat aagggcaaga cttcattatc atcaatcgat tcataaaatg
    21661 cctgactatc aaagtgttca gccatcattt ctgttaaact tgattttccg gcaccaattg
    21721 tacctgctaa aacgatcacg ctcatttacc tccagctcac ttcatcacta aatattgtgt
    21781 ttaactccaa cctttttaaa tataccccca atatctagta gtgtcaattg tatttttttc
    21841 tagtcaaaga agaaaaaaag tcaaaaaatt cttaagaaaa aagtgttttt tctttagaaa
    21901 acgtattctt agttttactt ctgagatggt tttagtaaaa taaaggagga atgttttttg
    21961 agacccttag tggacaaaaa aattcgtgaa gcagcgcaac tcgttttggg cgctttattt
    22021 tttgcttaaa aatcactttt acgtgagaag gagttagatt atggcaaact atggccaagg
    22081 agaatcaagc ggaaagatca tattgatggg cgagcacgcg gttgtttatg gagaaccagc
    22141 gattgccttt cctttctatg caacaaaagt caccgcattc cttgaagagc tggatgcaat
    22201 ggacgatcaa ctggtttctt cctactattc aggaaattta gccgaagctc ctcatgcatt
    22261 aaaaaatatc aaaaaattat tcattcactt aaaaaaacag catgacatcc aaaaaaactt
    22321 gcaactgacc attgaaagca cgattcctgc tgaacgtgga atgggatcaa gcgctgcagt
    22381 cgccacagca gtcactcgtg ctttttatga ttacttagca tttcctttgt ctcgtgaaat
    22441 actattagaa aatgtccagc tttcggaaaa aatcgcccat ggtaatccta gtggaatcga
    22501 tgcagccgct actagcagct tgcagccgat ttattttaca aaagggcatc ctttcgacta
    22561 cttttctttg aacatcgatg cttttttgat tgtcgctgat acaggaatca aaggacaaac
    22621 aagagaagcc gtcaaagatg tcgctcacct ctttgaaacc cagcctcatg aaactggaca
    22681 aatgatccaa aaattaggat acttgacgaa gcaagcaaaa caagcaatca ttgaaaattc
    22741 accagaaacg ttggcacaga caatggatga atcacaatca cttctggaaa agctgacaat
    22801 cagcaatgat tttcttaatc tattgatcca aacagcaaaa gataccgggg cattgggtgc
    22861 caaattaact ggcggtggac gcggtggctg tatgattgca ttagcacaaa caaaaacaaa
    22921 agcccaagaa atcagtcaag cacttgaaga tgcgggtgct gctgaaactt ggatacaagg
    22981 attaggagta catacctatg tttaaaggca aagcacgcgc atataccaac attgctctaa
    23041 tcaaatattg gggtaagaaa aatgaagaac tcatccttcc aatgaacaac agcctttcgt
    23101 taacattgga tgcattttat acagagacag aagtcatctt ttctgatagc tatatggtag
    23161 atgaatttta tctagatggc accttgcaag acgaaaaagc aacaaaaaaa gtcagtcagt
    23221 ttcttgacct ttttcgtaaa gaagccggcc tctcactaaa agcttcagta atcagtcaga
    23281 atttcgttcc taccgcagct ggattagcct cctctgccag cgggctagct gctttagcag
    23341 gcgcttgcaa tactgctctt aagcttggat tagatgatct ctctctttca agatttgctc
    23401 gacgcgggtc tggttcagct tgccgaagta ttttcggtgg tttcgtcgaa tgggaaaaag
    23461 gccatgacga cttaagttct tacgctaagc cagtcccttc cgattctttc gaagacgatt
    23521 tagcaatggt tttcgttttg atcaacgacc agaaaaaaga agtgtccagc agaaatggga
    23581 tgcgtcggac agtcgaaaca tccagttttt atcaaggctg gttagattcc gttgaagggg
    23641 atctatatca attgaaacaa gcaatcaaaa caaaagattt ccaacttctc ggagaaacga
    23701 tggaaagaaa cggactaaaa atgcatggaa cgactttagc tgcccagcct ccattcactt
    23761 actggtctcc tgacagctta aaagcgatgg atgcggtgcg acaattgaga aagcaaggca
    23821 ttccatgcta ctttacgatg gatgctggcc ctaatgtcaa agttttagtg gaaaacagcc
    23881 atttatctga ggtacaagaa acatttacca aattatttag caaagaacaa gtaattactg
    23941 cacatgctgg tccaggaatt gcgattattg aataaaggga gatttttatg attgaagtat
    24001 ctgcaccagg caaactctat attgccggag aatatgcagt tgttgaaaca ggccatccag
    24061 cagttatcgc tgcagtcgat caattcgtga cagtcactgt agaatctgca cgaaaagtcg
    24121 gaagtatcca atctgcacaa tatagtggga tgcctgtacg ttggacaaga cgcaacggag
    24181 aattagtttt agatattcgg gaaaatcctt ttcattatat ccttgctgct attcgcttga
    24241 ctgaaaagta tgcacaagaa aaaaacattc ttttatcctt ttatgatctg aaagtaacaa
    24301 gtgagttaga cagttcaaat ggccggaaat atggattggg ttctagcggt gccgtgaccg
    24361 ttgcaacggt caaagccttg aatgtttttt acgcgttgaa tttatctcag ttggagattt
    24421 tcaagattgc agcactagcc aatttagcag ttcaagataa tggttcctgc ggcgacatcg
    24481 ctgctagctg ttatggtggc tggatcgctt tctcaacctt cgaccatcct tggctccaag
    24541 aacaagaaac tcagcattct atcagtgagt tacttgccct ggattggcca ggtctatcca
    24601 ttgagccatt gattgctcct gaagatttac gtttattgat tggttggacg ggtagccctg
    24661 cctctacttc tgatttggtc gatcaagttc accgttcgag agaagataaa atggtggctt
    24721 atcagctttt cttaaaaaat agtacagaat gtgtcaacga aatgatcaaa gggtttaaag
    24781 aaaataatgt aacgttgatc caacagatga ttcgaaaaaa ccgacaatta ctgcatgatt
    24841 tatctgcaat cactggggtc gtcatcgaaa cgcctgcttt gaacaaattg tgtaatttag
    24901 ctgaacagta tgaaggagcc gcaaaatctt ctggtgcagg tgggggcgat tgcggaatcg
    24961 taattgttga ccagaaatct ggcattcttc ctttaatgag tgcatgggaa aaagcagaaa
    25021 tcactccact gccgttacat gtctatagcg atcaaagaaa ggaaaaccga tgaatcgaaa
    25081 agacgaacat gtttcactag ctaaagcctt tcatgataaa caaaaaaacg aatttgactt
    25141 cgtacggatc atccataacc ctttgccgca aatagcagtt tctgatgtcg atctaagtac
    25201 acaggcagtt gggtttacac taagcagccc attttatatc aatgcgatga ctggcggaag
    25261 cgaaaaaaca aaaaaaatca accaagatct tgcaatcgtc gcaagagaag cagatttgat
    25321 gattgccact ggttcagtca gcgctgcctt gaaagtccct tctttagccg atacttatac
    25381 gattatgcga caagaatatc ctcacgggaa aatcattgcc aacatcggtg cagggacttc
    25441 tgtcgaaaga gccaaagaag ctatccgatt gtttcacgca gatgccttac agattcattt
    25501 gaatgcacct caagagttgg ttatgcctga gggagaccgt gattttacca attggaaagt
    25561 acttattcaa gaaactcaaa cagccattga cgtccccctc atcgtaaaag aagtcggttt
    25621 tggcatgaca agagaaacgc tcaatgatct agctgcttta ggagttcata cagttgatat
    25681 cagcggtcga agcggaacaa gcttcaccca gatcgaaaac gcccgtcgct ccaagcggga
    25741 actgagttat ttagctgact gggggcaatc gacagtctct tcattgcttg aagcaaatga
    25801 agcagacact tctatggaaa tcctagcctc cggaggtatc cgcaatgcct atgacatatt
    25861 taaagccctt tgtctcggcg caaatgccgt gggtacttca ggaaccgtac tgacgcatct
    25921 gatgaatcac ggcgtagaag aaactattat actgatgaag caatggcaag aagagcttcg
    25981 tctgctttat actatggtcg gcgcaacgaa taccgcggcc ctacaccaac aatctctgat
    26041 cttttccgga ccagtgaagg attggtgcga agcaagaggg atcgacttag tgaagtatgg
    26101 aagacgcaca gaaaagagcg tgggacaaaa gtaaagaaca cttttgtctc acgctctaaa
    26161 cacgtacaaa cggcggaagt aaaagcaact ccttcggaaa taagctgaaa ttcacaaaaa
    26221 tttgaaaagc aattttcgtg aattccttct tatttcttgg agttaaatgc ttctgtccca
    26281 gcctcttttt tcgaataaat gatgtttaat cagctgtcct tcggtattcg ttcaatccgc
    26341 tgatttcaca acctctccac taaacagtgt actaaagctc ttttaaggtt gttttccgat
    26401 ataggcaagt atgcctccgt ccacatacaa gacatggccg ttaacgaaat cagatgcgct
    26461 agaagccaag aagacagatg gtccagccaa atcgattggc tcgccccagc gtgctgctgg
    26521 cgtacgtcca acgataaatt gatcgaatgg atgacgttca ccgttttctt gtgtttcgcg
    26581 taatggagca gtttgcggtg tagcaatata acctggtcca attccattac attggatatt
    26641 gtattgaccg tattcagaag cgatgttttt cgtcagcatt tttaatccgc cttttgccgc
    26701 agcataagca ctgaccgttt cgcgtcctaa ttcactcatc atggagcaga tattgatgat
    26761 tttaccgccg cctttttcga tcatatccgg aataactgct tttgaaacga tgaatggggc
    26821 atttaaatcc acgtcgatta cttggcggaa gtctgccgca tccatttcga tcatcggtgt
    26881 ccgtttgatg atccctgcat tgttgactag aatatcaact gaacctacat cttctttgat
    26941 ctgttgaacc atcgcttgaa ccgcttcttc atcggtcaca tcgcagacat aaccttttgc
    27001 atcgatgcct gcttctttat aatgcgctat accttcatca actgattctt gattcaagtt
    27061 attaaatacg atttttgctc ctgctgtagc taaagagcgt gcaatctcaa atccaatacc
    27121 atagacagca ccggtgacta atgctacttt tccatctaaa cgaaacatat ccatactaaa
    27181 ttccatcatt ttctctccct tatgcttatt ttaattgatc catttgcacc atatccatgt
    27241 ctgtataggt gatattttct ccgcacattg cccagatgaa tgagtaattg cttgtaccta
    27301 cacctgaatg gatcgaccag cttggcgaaa taatggcttg ttcattgtct acaactaaat
    27361 gttttgtttc atctggtttg cccatcatgt ggaacacacg tgtatcttca ttggcataat
    27421 caaagtagac ataagcttcc atacgacgtt catgtgtatg acaaggcatc gtattccaag
    27481 cgcttcctgg ttctaaaatc gtataaccca tttgtaattg acagctttcg catacatttg
    27541 gatggatgta ttggtagatt ttacgttcat tcagtgtcag gccttcacca gtctccattg
    27601 gttttacttg gtcgatactg atttttacat ttggatattt atgatgagct ggaacagaac
    27661 taatatagaa tttagctggg ttttcagcat cttgagaaga aaatacaaca tgtcgtgttt
    27721 cttttccgat atagtatccg tcttgttttt tcatttcttc tttttgccca tcgatctcga
    27781 tgaatcctgg tccaccgata ttgatcacac ctagttcacg acgctctaag aagtaatcaa
    27841 cacctagttc tttgtctaat gtgatttcta attctttttc agtaggtgtc actccaccaa
    27901 agatcattcg atcgttgtgc gtataagtca aactgatttt ccctggttca aatacctttt
    27961 ctactaaaaa ttcttttctc aattgttccg ttgaataatg acggatatct tctggactgt
    28021 gtgtataacg tgtttccatt tcttgcattt tcttctactc cttctatcga atgatttttc
    28081 cagagccgca tgctaaaaat tgtgcgactt cttcttgact aaattggttg cagtccccat
    28141 ggatcgtatg cttcaaggca gaagctgccg tagcaaaatc aatgatttct tgcggagaca
    28201 tattggataa aatcccatgg agcacgccac ctgcaaaagc atctccgcca cctactcgat
    28261 ccacgattgg ggtgattcta tggacttggg attccacata ttcatggttc atccacaatg
    28321 tccccttcaa ttcattttca cttgctgaat gaacggtacg cttggtcgaa taaaacacct
    28381 gaatattcgg gaacttttcc tgcatttttt gataataata aattagaggt tgttcttctg
    28441 cttctggcgc tttctcgata cctaacaaat aaagagcatc catttttcca gctgagcaga
    28501 tatctactaa aggtaaaagc tgatggatta cttcgctggc ttcttgctgt gtccataatt
    28561 ttccacgata gttgatatcg aagctgatca ggcagccagc tttttttgca gcttctatca
    28621 cctgtttcgt cattttttgc cattttttag acaaagctgg ggtgattcct gaaacatgaa
    28681 atatatctat tccttggaat aactcctcta gcgaccattc caaatgatcc atttcagcaa
    28741 aacttgatcc ggcacgatca tagatcactg aggcggcgcg ttcgccacta ccagtttcca
    28801 tataataagt acctagtctt gatccaccag ataatacctg tttcgtatct actccataac
    28861 attgcaagtg ctttttgact gcttttccca atgcgttatc aggtactttt gtagcaaaaa
    28921 tcgcttcatg tccgtaatta gctaaagaaa ttgctacgtt tgcttcccct ccgccataat
    28981 gggcagcgaa tcgttcactt gaatcgatac gggttccctg ctctgtggac aatcgcaaca
    29041 tgatttctcc gagtgtcact acttttccca taagcagcta cctcttgatt tctttcattt
    29101 ttgctgcata ctgttgcgcg acttccgtta ctttgtcaaa atctcctgta gctgcgggtg
    29161 ctaaaaggtt cccacctact ccgactgtta cgacaccagc atcgaaccac tctttcatat
    29221 tgtccagact cacaccgcca gttggcatga tattgacttg aggcatcggc gccttgaagg
    29281 cagaaatgat acttggtcca taagcgcttc caggaaatag tttgacgata tctaccccgc
    29341 ttttcagcgc tgttttgatc tcggtaatag tcatacatcc tggtaagtaa ggaatttgat
    29401 acagattgca catctctgcc gtttcttgat caaaggaagg gctaacaata tactctgcgc
    29461 cagctaagat agccaagcga gctgtgacag cgtctaatac tgtgcctgcg ccaatcacta
    29521 tttctggttg gtctttgtag aatgacaaaa gttctttgat tacttgatct gcttgaggga
    29581 ctgtaaaagt caactcaata ccagttagac cacctttgat gattgcgtgg caagcattca
    29641 aagcttcttc tttgcttttt ccgcgtacca ccgcaatcac tccggcgttt ttcaatcgag
    29701 ataaaatatc aatttttttc atcttctcca cctatttcct atttagaaat tttattttat
    29761 aacttggaaa tctataccca tattatacgg cagaaaagaa aaattgcaaa cgcttttttc
    29821 attatttttg aattctttgc tcgatttcgc tttttgtttg cttgagggca ctgattattt
    29881 gttcttttgt ctcatctgtt aaacgatatt tcggcatact tacactaaat gcaccaatga
    29941 tttgttcgtt tttcacgatt gaggctccta tacaaaaaat atcacgttcg atttcttcat
    30001 cgtcaaatgc gatgtgggtt tcttttaccg ttttgatttc ctttttcaac cgtaatgcat
    30061 tggtgatcgt atattctgtg taagggatca acggatgctt ttcaagataa tcagtgtact
    30121 gctcttcact gaattctgct aatacggctt tgcccatcgc agaattgtat aatggtcggg
    30181 tgattccgat ttttgaagac atgcggatcg tttgatgttt tggatctagt ttgttcacgt
    30241 acaagatctc attgttgtcc aataccccta agtggatagt ctcatcgatc ttttgctgca
    30301 gctgttctaa aaacggtaaa gtcaactcta ccaaatcgat ttgttcaatg tttttattcg
    30361 cgtagcgaat aagctttgca cctaaacgat agtttttctc tgaattttta tttacgtagc
    30421 cgatcatcaa caaagtatct agtattttca agactgtaga agaggtcatt cccacaccct
    30481 ttgcgatcgt ctgcaaagag acatcttggt tttccgctaa atagtctagt attttagagg
    30541 ctttgatcaa tacagttcca taaggctttt tattttccat ataatattct cctatttctt
    30601 tataagaaaa attatagcgg aaataccatc aatttaaaag agatatccag atataaacta
    30661 ccatttctct ttacaggcaa tttaatgttg aagatccgct tcgtttttac atcaaacttt
    30721 gatataaagc aatgatttct tctactgtcg tttctcttgg atttcctggt gtgcatacat
    30781 ctcgcaaagc atcttctgca attgctggaa tatctgcttc ttttacacct aattcagaaa
    30841 tggtttctgg gattccaacc gctttgctca accgatcgat ctcaccacaa gccgcttctc
    30901 ttacatcttc taaagacatt tcagctgctc ctttaatccc tagaacaaga gcgatctcac
    30961 ggtacttttc tccagtatat tctttgttat atttcatgac agttggcaaa agtgctgcac
    31021 acgctacgcc atgaggaatg ttgtaccatg cacttaacgg gtgagccatc ccgtgtacca
    31081 gtcctaaacc gacatttgag aagcccattc ccgcaatata ctgtcctaat gccatttttt
    31141 ctcgcccatt ttgatcgcca tctacagaag cttccaaaga atggccgatg atttcgattg
    31201 cttttaaatg cagcatatca gtcatttccc aagcgccttt tgtgataaac ccttcaatag
    31261 catgcgtcat agcatccata cctgttgatg cagccaattt tttcggcatt cccatcatca
    31321 tatcgctgtc gatgaacgcg acgattggga tatcatgagg atcaacacaa acaaattttc
    31381 ggtgatttac ttcatctgtg atgacgtagt tgatcgttac ttctgctgct gttccagaag
    31441 tcgttggtat agctaatata ggtaaacatg gatttttcgt atcagcaaca ccttctaaac
    31501 taatgacgtc tgaaaactct ggattcgtca aaataatccc aatggctttt gccgtatcga
    31561 ttggcgaacc tccaccgatt gcgatcaaac agtctgcctc tgcctcttta acaaaagcca
    31621 ctccatcttt tacattttgg atcgttggat taggtacgat cccgtcgtat aaagcataag
    31681 cgattgctgc tttatctaga agttctgtta cttttgttgc tataccgtgc tcgatcaatc
    31741 ctttgtcagt aatgacagct gctttgccga atcctcgctg ctgaaattca gggacgatcg
    31801 actcaatcgc tccttttcca aaatacgatg tttcatttaa aatcattcga ttactcattt
    31861 tctattcctc ctaatttgta ttatttttca caataaaaac gaaagaaccc caagcaaccg
    31921 gttgcccagt tgcttgttgc tacagttcaa agactggcat caattcccaa ctgactggag
    31981 aggcatctgg atttgtctcc atgatatctt ccatgtactt ccaccatttt tgattgatag
    32041 atgtatttgc cattttttcc cataactttt catcttcgat cactaagtaa ccaaataaca
    32101 aacttgtact cggatcaaga tagatcgaat agtcgatgac cccgtgttca cgaagtgctg
    32161 tacgcatttc tggccaaagt tcatcatgtc tttttttgta ttcttcttgt ttttctgggt
    32221 aaactttcat gcaaaaagct tttttcacca tgtcagacac ctgctttcgt atgaacttcc
    32281 tccaaataac ctgcttttgg agtgacacca aacgcatctg ctaaatgcca caggttttcg
    32341 tccgtgattg tttgcaagat tggtccttgc gctttgacgt acgtatagac ttctgctgcc
    32401 ttttctgctg tctcgatcaa accaaagact tcatccatgt cttttcctga cccgtagatt
    32461 ccatgttgtg gccataaaac caatcgagtt tctttcattt tttcagctgt tgcttcccca
    32521 atctcatttg ttcccggaac catccacgga atgatggaaa ttccttctgg aaaaacaact
    32581 aagcattccg tacacatttg ccatagcgta cgactaaagg aacgttcgtc taattcatgc
    32641 gtaaaactca tcgctaatag atgactagca tggcaatgca taacgatccg attatctggg
    32701 tctacttcta aacgggcgat atgactcatg aaatgacttg gcaactcgct tgtaggaagg
    32761 cattcgtttt ccagccccca caaaagttcc agtgttttgc catcttcagc aactcgaacg
    32821 attccaagat tctcggcagg ttcgctcgct acatttttaa aatatcttcc tgaaccagtt
    32881 acaataaaat agcatcctgc taaacggctg gcatcaaaaa tcatcgagat gcggcggatc
    32941 actttttcag gatccaagaa cggtgtgatc tcttcttctt ttagtaaata agaaatattc
    33001 ccgccatttc tttcatccca tcccaagcga taaagattcg acgtcgtttc gatcatttct
    33061 ttcacataag gcgcttgtaa aatatctatt tttttcaata tagccacact cctattttct
    33121 taattccaac acgtcttttt gatattcctg gacttcttcc agccaatcca aaccaactgg
    33181 aacttgattt ttgtaacaga aataattcca tacatcggca tacgggaaat ctttcaattc
    33241 ttccgtataa gccattcttg aagtaaagtc aaaagccagc tccatttctt tcaatcgctc
    33301 aactggttcc agcatagctt tcagcagtgc tttttgtgta tttctcgtac cgatgaccca
    33361 agcagcgata cggttgatcg ttgcatcaaa gaaatccaga ccgatatttg ttttttccaa
    33421 caattcgtta cggactaatt ctttagcgat ttcctgcaat tcatcatcca tcaaaaccac
    33481 atggtctgaa tcccaacgca ctggacggga aacatgcaac atgattcctt tgctgaataa
    33541 agctaatgaa gataatttat tggaaatcac ttctgttgga tgaaaatgtc cagcatctag
    33601 acaaacaagt ttgtcacgag tcaacccgta acccatataa aattcatggg aaccgactgt
    33661 ataagcttca gcgcctaaac caaatagttt gctttctact gcatcttgta cataccgttc
    33721 atctaactct tctgtaaaaa tttcatcgag tgattccatt aaacgttccc gtggggtaaa
    33781 acgatcaatc ggattatctt tcatcccatc aggtacccaa aagttattga tacttgtttg
    33841 cccagtttct ttcccaaagt actccgcgat tttgcggctt cttttcccgt gttcgatcca
    33901 gtattcacga acttctttat ctggatgagc caatgtaaag ccgtctttca tcattggatg
    33961 cgaaaagaaa gtcggattga agtcaagacc gatttcttct ttttttgccc actctaccca
    34021 tttttcaaaa tgtttcggct caatttcatt tagatcgaca gattcttctg tatccaaata
    34081 gatagcatga aggttcaatt tgtgtttgcc cggtatcaac gaataagcct tctccagatc
    34141 ttgtctaagc tcttcaggtg ttcgtgcggc tcctggaaaa ttccctgtcg ccatgattcc
    34201 accggtcaat tcgccatccg gtgtcaagaa acctttgaca tcatctcctt gccagcaatg
    34261 catagaaatt ttgatgtcct gtatcttttc tagtacagca tctgtatcta caccgatagt
    34321 tgcatatttt tcttttgctt gttcgtaatt tctttcaatc gtgttcatac gattactcct
    34381 ttgattttat tcacgtacca ttccatcgtg ttttgcttat tttcatctgg gtaaaaagtt
    34441 tcacatggaa acgactgttt gattacattt cttgcttgtt ctatattcga aaactgacct
    34501 gtactgagca tttgcatggc aacattcccg attgctgtgg cttcactagg tcctgcttct
    34561 acaatgattc cggcataatc tgctgtcaat tgattcagta gacgattatt cgatccgccc
    34621 cctacgatgt gcagcttttc caatttcgtt ccagttaagt gttctacttt gatcagctct
    34681 tgtgcgtaac aaagagcaag attatcatac acacaacgag cgatagctcc cgatgagaca
    34741 gggactggct gctctgtttc aaaacagtat tgctgaatct cttctatcat atttggggga
    34801 ttcaaaaatc gagggtcatt gatttccatc acttgctgca gcggtttttc ttttgcagca
    34861 agctctgcca attccgcata actgtactcg tactcttgca ttcgggcaac ttcctggatc
    34921 aaccacatcc ccatgatgtt tttcaaaaaa cgcaccgtgt tatgggcacc ccattcattc
    34981 gtataatttt cagaaaaagc attgcgagaa acattggcga tctttgtttc aatccctaaa
    35041 agcgaccatg tcccactgct gatataaccc caacgttctc cttgacctgg cgttcctaat
    35101 acagctgacg ccgtatcatg acttgctact gtgataaagg tcacatctgg caaatcatat
    35161 tttgaaaatt gttctttttg cagatctcct agtatcgttc ccggctctgt caatggtgga
    35221 aataattcct tcgagacacc gattttttca agaagttcta tttcccattc ttttgtccct
    35281 gcattcagca gctgcatagt agatgcatta gttttctccg tcaccatgtt accagtaaag
    35341 acatacccta aataatcagg tatcagtaac aatttatgtg cttttgccaa attttctttt
    35401 tcttcagcaa acaattggaa caaggtattg aaaggctgga tctgaatccc tgtctgctca
    35461 tacaacttat ctagagaata gatttcagaa aactgttcta ctgctccatt ggtacgcttg
    35521 tcccgataag caatcggatc atttatccga tccccttgtt catcaatcag acagtaatcg
    35581 actgcccatg tatcgatccc cgcaaaaagg cgatcaatcc ctgtctgctt taatttttca
    35641 aatccaatca aaatctcttt gataagataa tcgatcttcc agcgatcgtg accgtctttg
    35701 ttccgaaagc cgtttttgaa ccggtgtact tcttccaaga tcaaagaacc ttttgcatcc
    35761 ctcgtacttt tcattagtcg tccgcttgat gcaccgatat cgatggcaac aaatgttgga
    35821 ttcatttctc cacctctttt actttattgt tgattagcac tttattaata aagcgttatc
    35881 atttattgac aatgctatca taatacagaa tcttatattt tgcttcacac tattttggta
    35941 tataattgca ttatcttgac aagttgggag tgtttacatg gattactggg aaaaagccaa
    36001 ccactcatgg tcggaagact caaaaagagt gatcttgacg cctacacaaa gaagcaggaa
    36061 tttatttttt tatatccaag aaatcggtta cttcaaagca ccaatccctt actatactga
    36121 acgagcacat ttaccttctt acttgataaa gtttactttg tctggcaaag gttcgcttac
    36181 atataatgat aatcagcaac agctaaaagc tggagactta ttttttattg actgccaaga
    36241 atatcagcgt tatcaaacga tcagcgaaga accatgggaa atggattgga tccatttgaa
    36301 tggtcagaac gtccaatcat tttatcagga gttcacaaaa gacggccata acaccttcca
    36361 ttcaacagga gatccatcag aaaacaagat ccaccatttg atcacagaat tgatccacat
    36421 gcaacaaggc acgaatgccc gtaccgattt ccagtcctct gtagctatcc acgagttgct
    36481 gaatgaactg attttgcaaa agtatcagct agatttcgaa gaagaggata ttcccgccta
    36541 cgtttacaaa ctgaagcaat acttagacga acatttcaaa gaaacgatta cacttgatca
    36601 attagaacaa caattcttat tgaataaata tcagctgagc aaagattttt ccaaatttat
    36661 cggttctcca ccaatcgatt atttgatcag caaaaaactt tcttacgcta aagaccttct
    36721 tcggtataca gaatttacgg ttcgaatgat ttccgaagaa atagggattg agaattttgc
    36781 ttactttagc cgcctgttca aagcaaaaac aggactatcg ccgagagact ttcgtaaatt
    36841 cagctgattc atataatatg tacgtcattt tttcaaaata ttttgaaata tatagataaa
    36901 aataaaataa ccgcagtttc tgcttctatg taaacaacag cagctactgc ggttattttt
    36961 tagtgatcat tctcattgtt catcttcaaa atgttcataa tgaaccaact gtttctgtgt
    37021 atcacgtttc aaaaattctt taggcgttat gccgaactct ctcttgaatg ctttcagaaa
    37081 atgcggataa gaagaaaaac cacttttact atatacagca ctagagctgc ggtattctcg
    37141 caacaagtgc ttggcataga tcagcttttt cttcattaca tactgatgaa acgtaaatcc
    37201 agtgtacttt ttaaattctc gtgtgatatg gtatttgctg acaaaaaact gcttttccat
    37261 ttcctctaaa gaaagcggat gagataaatt ttttgagata tattgtaaca tttgttccac
    37321 acgctcatta ggaactacag cattctgttc ttgcgcactc tcttcttttt gtacaacttg
    37381 attgagaaaa atcaagtatt ctaccagagc ttgttcatat tttatgtctt caccgtaaat
    37441 ttttttatca ggaggatggt cgataaatga taatttttgc ttcaatactt cctgttctat
    37501 ttgcaataca cgactcctct tctctcccat tggttgaaaa caagccgata gattagacca
    37561 tggcgtggaa cgactttcta gaaaagaagg ggtgacaaag atatagacac gctcgaatac
    37621 atctgaagat tggcggacga tacgatgcaa atcattggaa tggatgagca gcacacttcc
    37681 tgctttgatc gtaaattgtc gtccgttcaa ataaaaagtc gcttctccct ttaatgtagc
    37741 atggatctca tagaaatcat gatgatgata gatcgtttta ttttggctga cgacatcttt
    37801 gacatgatag atttcatact gatcagaaac catcgtattg acttgttcac cgctgatttt
    37861 cttttggagc atgtgccgca cctccttttg ttcacttatg cacaatttta ttataccacg
    37921 ttttttgtaa tcaaaaagag ctgcttttat atactcttat aaaaaagaag agacgtggca
    37981 tcttctagcc ccgccccctc cattctaaat attagttaac caacgtcttc tttacattat
    38041 tcaagaagac ttcttccata acgaatccca tcatcttttg tttcttcaag aaccacatac
    38101 aggtacggac gattcttcat gatgatttct ttgatttttg tgtactcgat cacaccgtct
    38161 cccaaattta ccggcacgat tttttcctgt tcgacacgaa aatctttcaa atgaaccgca
    38221 catatctttt caccaaaacg ttcaaaagct tcttctacca attggacttg ttcttgataa
    38281 tcactacttg tgatcaaatt ggtcggatct aaaatgattc ctagatagtc cgaatctact
    38341 gcttggatca gctgctctac ccttgctaaa gaatacaccg gatgattcaa acctggttct
    38401 atccccacca tcatttggta tttttctcca gcttttacca agcgacggat aacggacaca
    38461 gcttcagcaa atgcttcatc tgtgaaattt tcttccgtat attggatttc cggcaaaaca
    38521 catccagttt ccgaagcaac catcgacgca ccaaaggaag aagcatagcg aagatactgc
    38581 tcgaactttg tcagcaactc ttgtcgtatc gttaaatctg gatgaatcat attgatatag
    38641 caacttaaaa ttcctacggt cacttcttgc ttttctaaaa ctcgtttgac ataatttccc
    38701 atcccagaat tgatctcatt tgcgccagaa ggtatttcag gaaacgaaag acctaaggcg
    38761 agctgcagtg tatgaatccc ttgttctttt gttttttctg caagatcttc tatcgtttgg
    38821 acttcggaaa gatcatgtcc tctaattgta atgttcaaca taactattct cctaactcag
    38881 catattgggt agccacatga cgatttgcgg gaagtaagta atcaaaaaca agacgactaa
    38941 gataacccca taaaaaggaa gaagcggttt cagcattgcc tctgctttta cgcctcctac
    39001 acttgcaccg acatatagtc ctgtacctac tggcggcgtg atggagccta cacacagatt
    39061 catgattgaa aacaacccgt aatgtacagg atcaacccct acacttgtga cgattggtaa
    39121 aaatattggt gtaaaaatca agatagcagg cgccacatcc ataaacatac ccaccagtaa
    39181 taacacaaga ttgactaata acagaataat aaatttatta tccgttacgc ccataatcaa
    39241 cgaactaatt gcctgaggaa taccggtaaa cgccatggcg aaagacatca tggaagaagt
    39301 agccaataag aacatgatcg tccctgacat tgctactgat tgaaacaaca tattaggtat
    39361 atcttttagt tttaccgttt tatagtaaaa catagcaatc aacagggagt aaatgacagc
    39421 tatcgctgaa gcttctactg ctgtaaatat ccccgtcaag ataccaccga taatgatgac
    39481 aatcaataga aggctaggaa tcgcctcttt tattattttt ccagcatgag ccatctgtcc
    39541 tttttcgaca acagggtatt tattcttttt agcaacgatg aatgctacta gcatgattcc
    39601 tagcgcccac aaagcgccta cgagataacc tcccataaac aaagcagaga tcgaagcacc
    39661 accagcaaca agtgaataca tgataaaagc agtacttgga ggaatgacca tacctgtcgg
    39721 agctgacgcg atattgaccg ctgtcgcaaa cttccggtca tatccttctt ccacttgctg
    39781 agggatcaac actccgccaa tcgcggtaga agcggcaatg gccgagctag agatggaacc
    39841 aaataaagca ttccctaaca cattcgtatg agccaaagcc cccggcacgc gcccgacgaa
    39901 caacatcgca aaatccacta gtttcttagc tatccctcca ttattcatga taatgcccga
    39961 gagaacgaaa aacggaatag cgatcagcga aaaactattg atacttccca ccattttttg
    40021 agctgaacta aaagcagaaa catcaaacgg tataactaac aataaagtca tcatcgatga
    40081 gaatgcaata cttatagcaa tcggcatccc taatacaagc aacacaacaa atacaaaaaa
    40141 taacactaat ccggcttcta ttgccatttt attcctccat ttttatattt tttcttgaat
    40201 tgctggcttg ttgcttaatg tatcgattag attgatcagg ctatagataa tgatgagtac
    40261 ccccgataca ggtactgaca gataaacata tcccatagga atacttaaag aaggcgagat
    40321 ctgccccatt gtcaatgtga cagcattcca tccgccatac accatgatca tcgcagcaaa
    40381 taccagaaat aaactttgaa caacaacttc taaaatttta ctttttttgc cagttaattt
    40441 ttcacttaaa accgttacgg ctaaatgtgc tttttgtccc acaacatacg cggaagacaa
    40501 catggcaaac cagacaagac cgaatcgaac aagctcttcc gtgaccgtat ttggatttcc
    40561 taaaaccgtt ctggaaaata cttgccataa aacaacagcg accatcccaa caagcaaaac
    40621 agctcccagg atctctaata ccttgtttaa aatcattttc atcacagcta cgctccttgc
    40681 tcttggatct tttgataaag gtctttgtag gtaggatcgt ttttgaatat atcatgaagt
    40741 ggggcaactg cctctcgaaa cggtgtatta tctacatcat tgaattggac tccatgttct
    40801 tcttgtgcta aagctttttc ttcttcaatt gcttttgcaa aagcttcaat ttcaaaaacc
    40861 gttgattctt tcgctgcttc ttcaatggct ttttcctgct catcagttag ctgttctttg
    40921 atggcatcat tcatgatgat gatatctggc acacgtgtat gctcatcata ggaatagtat
    40981 ttagccacac caccatgtcc agctgtatac aagacaaatt cattgttttc tgatccgttg
    41041 atcaaattgg attgaagaga cgtgtacact tcatcacttc ccataggtac gggcgaaccg
    41101 cctaaaagct ctaccatttt gatagcggtt tcgctttgca tcacacggat cttctttccc
    41161 ttcaaatctt ccggcgtatg gatcggaccg tctaccatat aaaagctgcg ctgaccagaa
    41221 tcataatagg tcaatccggt aaatcctaaa tttttggtcg attggtagat tggctccatt
    41281 atttcttcat tatccatcac cttaaagaaa tgttcttcat cattgaatag ataaggaact
    41341 gaaaaaatcg aataatcttt agagaaactt tccaacgctg aggcacttac ttttgtgaaa
    41401 tctactgctc ctgactgtgt caattcgatc agttcacgtt ctccacccag ttgtccatct
    41461 gggaaatact ggatctgtac ggaaccatct gttttttctt ttactaaacg cccaaattca
    41521 ttcattgcat caattactgg ctggctgttg cttgcataag caaagcgcaa ggtaactatc
    41581 tcatttacat ctgcatctgc gacagttgcc acactagaat tatttccgca gccgcttaga
    41641 cctagcagac ctaggctaag cgaacttgct gcaaacagca gtgatgtttt tttcatatgg
    41701 gtttcctttc ttttaacaat aagagacgag caattctgtc aaacaaagca ccgttaacga
    41761 ctgtccataa ggcattgctg tgattccgat attccgataa aaatctaaac tgtcccccat
    41821 tcctgtcccg attgaaacgt tttgaacttc tcctttttca tcgatttgtt caagcaatcc
    41881 ttggatagca cgataagcga cttctttata ttcttgtggt aaataacgtt tatggaccgc
    41941 tttcaaaata ccgtaagcaa atcctgcagt tgcagaagat tctacgtaag aagaaggatc
    42001 atccaacaac gtatgccata aaccgctttc agactgataa gtttttaatg cacgcacttg
    42061 tgcttcaagt gtttcgatca ataatttgcg caatgcatct tctttcgata attctaagat
    42121 ttcaatgatc tctggaatag caatcgtcaa ccagcagttt ccacgagccc aaaatgcttc
    42181 ggcatagtta tgatttcctt caaacgtcca gccgtgatac cacaatcctg ttcgtttatc
    42241 catcaagtat ttgatatgga tcataaactg gtaacgagct tcttctaaat attctgggcg
    42301 attcaacagt ttcccgattt ttgccaaagg aagcactgtc atcatcaatg tatcgtccca
    42361 caactgattc ttattttccg gtccatatgt ggcatgctgt aacccgtctt catttgtccg
    42421 aggcatgtca ttcatgaccc attctgccca ttgttctaaa taaggcaaat agcgtgaatc
    42481 tttcgtgtct tcatacaagt aagccatagt caatagcggt gccattgtat tgacgttttt
    42541 gggcggagcg ccttcttcca tacgatcctc aaaccattca ttgaccactt gataagcgcg
    42601 aggatttttt gtcatctggt aatttttata aataccatat agaccgacac cttgtggcca
    42661 attccacact gtccagcttt tgtcatctac tttcagtcca tcaaagtcca acaaaaattc
    42721 accattatcg tctttgatcg ttgtcaggtt ttcaacgagc aggtcaattt tttctttcac
    42781 taattcttta ggtacaaaaa tctcttcttt aataatcatc tttacgccac ctttttaaac
    42841 gctttcattt tatatcctta atatagcagt aataccgtcc actataaata tcgctttttg
    42901 aacaaaaact gtctaaaatt gacctaaatg ttaaataaca aaagtaactt tcagttactt
    42961 ggaaattaaa aaaagaactt ggagaaacaa cgttttccaa gttctttttc atctattttc
    43021 ttatcttttt tctacgcttt cttttgctta tccgttgtaa gcatcaatga tgatctgttt
    43081 caattcagag atcaatggtt ctttcggatt agctgtcgta cattggtctt cgtaagctaa
    43141 ttcagccata cggtctacgg ttgtatctag tgtttcttgt gtgactcctt gagcttttag
    43201 actcatgttg atgccgacag attttcctaa ttcataaaca gccgttgcta aagcttcgac
    43261 taattcctct gttgtttcgc ctttcagacc taagaatttt gcgatatctg cgtaatctgt
    43321 atctgcacgg aaatagtcat atttagggaa catcgcatgt ttttgtggat cttttgcatt
    43381 atagcggata atatgcggta aaagaatcgc atttgtccgt ccatgaggga tcccgtattc
    43441 cccgccgatt ttatgtgcga ctgagtgaca aatacctagg aatgcgttag caaacgccat
    43501 accagccatt gtagaagcgt tgtgcatttt ttcacgtgat tccatatcag gtgtttttac
    43561 tgatttttct aaatagtcaa agactaactt gattgcttgt aggctcaatc cacgtgtata
    43621 atctgaagcc atgactgaaa cataggattc gattgcatgt gttaacacat ccatcccggt
    43681 atctgctgtg acagatgctg gaactgacat gacgaattga ggatcgacga ttgcgacatc
    43741 tggcgtcaat gcataatctg ctaacggata tttcacatgt gtttcactgt cagtgatgac
    43801 cgcaaatggc gttacttcag aaccagtccc tgatgttgtc gggatacata cgaattgtgt
    43861 tttttctggt ttgtcgatct tatatgtccg tttacggata tccaagaatt tttgttttgc
    43921 accaaagaaa gatgtgtctg ggtgttcata gaacatccac attcccttag ctgcgtccat
    43981 tgcagaaccg cctccaagag cgattactgt atctggcttg aagtctgcca tcagtttcgt
    44041 tcctgcgtat acggtatttg tcgatgggtt cggttcaaca tctgagaaga cttcgatctt
    44101 cacatcattt ttacgtcgtg ccaatacttc acgaactgta tcgcagtaac cgaattggac
    44161 cataccaggg tcacaaacga tcatgacacg ttcaacgttt tccattttct ctaagtacaa
    44221 tagagagttc ttttcaaaga agatttttgg cggtaatttg aaccattgca tgttatttct
    44281 ccgtttcgct acagttttga cattgattaa gtttacagca gagacgttac gagaaacaga
    44341 gttcttacca taagaaccgc atcccaaagt caatgacgga atcatttcgt tatagatatc
    44401 accgatgccg ccttctgcag atggtgtatt cactaaaata cgacaagctt tcattcgaag
    44461 accgaatttg acatgcaact cttcgttttc tgaatggata actgctgtat gtcctaatcc
    44521 tccaagattc aacatacctt cacacaaatc aaacgcatgg tccgtgttgt tagctttgat
    44581 catggcaaga actggagaaa gtttttctct tgatagcgga taatctgggc ctacgccttc
    44641 caattcagca atcagcattt tcgttccttt tggtacactg attcctgcca attttgcgat
    44701 ttccatcgca gaatgtccaa cgattgatgg attgaccgca tattttcctt cgttcatcac
    44761 tgcatcttct aatttttgca attcatctgg ttttacgata taaacttggt gtgcttggaa
    44821 ttctgctttt actgctgcat agatttcttt atccacgatc acagcttgtt cagaagcaca
    44881 aatcattcca ttatcaaatg ttttcgaaac gatcaaatcg ttcactgcac gtttaatttt
    44941 tgctgttttt tcaatataag ctggtacgtt acctggacct acacctaaag ctggttttcc
    45001 tgtagagtaa gcagacttga ccattccagc tccacctgtc gctaagacga tcgcgatacc
    45061 tggatggttc attagcatag aagtcgcttc gatcgaagga tgttcgatcc attggataca
    45121 attttcagga gctccggcag cgatagcagc atcacgtacc actcgtgctg cttctgcaga
    45181 agatttttgg gcacttggat ggaaagcaaa aacgattgga tttcttgttt tcaacgcgat
    45241 cattgatttg aaaatcgttg tggaagtcgg attcgttgtt ggagttactc cgcaaaccac
    45301 accgactggt tcagcaattt caatcaaccc tttttggaca tcttcattga tcacaccaac
    45361 tgttttatca tgtttgatat tatgccaaat atattcggac gcatacatat ttttgattgc
    45421 tttatcttcg taaacaccgc ggcctgtttc ctctacagcc atcttcgcta atggcatatg
    45481 ttgatccaaa gctgccattg ccatttcatg cacgatatgg tctacttttt cttgatcaaa
    45541 gctttccatt tctttcaatg ctgcatttgc tttttctgcc aaatcatcaa tcattgcttg
    45601 tacgtcaatt gtcttcgttt tttctttttc tattacttta gccatcatta tcctcctaat
    45661 gggtcatggt tatttgttaa ataattcaca tgtatatcat acaatattta tttttctatg
    45721 taaacacttt tttgtgattt ttttcacagt tatttttaaa tataaaattg ctttatttac
    45781 aggtgtttgt atagaaaaat atataaagtt atttattatt atttatatga cacacccgat
    45841 tattcaatat tgagcaatat atcccatgct cttatctctt tcttttttga ataaaaaatg
    45901 aggtaggaca tctttctgcc ctacctcgtc tttaaataag ctttgatact tatttttctt
    45961 ccttctcttc tgtctcttcc agcgtttctg ttgtttcagt ttttacttca actgtctcag
    46021 ctggttcttc tgttgtttca acagtagtcg ttgcatcatt tgttacgaca gtccctggtt
    46081 tgaccgtttt gattgctgca cgatcgtact caaggaagat cccttcgcag tctaaaacaa
    46141 ctgtccgttt gtcattgtcg atttctgaaa caactccatg taatccgccg atcgttacga
    46201 ctgaatcacc tggtttcatg ctatctaaca agttttgacg ttcttgctgt tgtttctttt
    46261 gcgaacgtga cataaaaaac cacattgcta atagcgcgat aaacatcaca agcattggta
    46321 atgttcccat ttttttcacc tcactaattc tgtatctaca aaaaacactt tagcagaaaa
    46381 atgctgataa cactagtttt ttacttaatt tttaaaagat tttcctttag aagctttttg
    46441 cgttttcttt attgaacccg tattcttcaa aaaaggcttc tctaaattca agcaaattat
    46501 catccatgat tgcttgacgt acttgtttca ttagattcaa caagaagtat aaattgtggt
    46561 aagaagtcaa acgaatccca aatgtttcat cacatttgat caaatggcgg atataggcac
    46621 gtgtatagtt tttacatgta taacaatcac atttctcatc aattggacgg aaatcatggg
    46681 catatttcgc atttttcact actagacggc cttgagaagt catacatgtg ccatttcgag
    46741 cgattcgagt aggcaagaca cagtcaaaca tgtctactcc acggataaca ccatcgatca
    46801 atgaatcagc agcaccgaca cccatcaagt aacgtggttt gttttcgggg atcaacggtg
    46861 tagtgaattc taatacacga ttcatttctg atttaggttc cccgacagat aacccgccga
    46921 ttgagtaacc agggaaatcc atcccgacta gatctttcgc gctttgacga cgaagatctt
    46981 caaaccctgc tccttggatg attccaaata atccttgacg gtctggatta gcatgagctt
    47041 tcaatcctcg ttctgcccat cgagacgtac gttctactga ttttttcacg tagtcgtaac
    47101 tttcatcgaa tggtggacat tcgtcaaaac tcatcatgat atcagagccc aatttatttt
    47161 ggatgttgat tgctttttct ggtgataaaa acatacgcga accgttcaag tgatttttga
    47221 aatgcacgcc ttcttcttcg atattccgca tatcacttaa tgaaaaaact tgaaagccgc
    47281 ctgaatctgt taaaatcggt tgatcccagt tcataaattt atgcagtccg ccagcttctt
    47341 ctactaagtc ttcaccaggg cgtagccata aatgatatgt attacttaaa ataacgccag
    47401 cacccatttc tttcaactct tctggggaca ttgtttttac tgtagctaat gttcctactg
    47461 gcatgaacat tggtgtgggg aaagttccgt gtggcgtgat caattcccct aaacgcgcgc
    47521 cagtatgttt ttctttcttg attaaacgat agcgaatggc aggttgcgtc attcatttcc
    47581 ctactttcta tataaaatat cttgacaact cccaccatca taatcgtttc aagtgctttt
    47641 tgcaagttcc ctcggcttcc ttctacttta tttaatattc ttttttttat ccttttttta
    47701 tgaataacaa aaaaaagaaa aacattatga cagaaatatt ttttgataca tggtatattg
    47761 gtgttattaa agaaaaggag ggaaaaatca tgcaatcttc tgcagaatta aaacgccaag
    47821 caaaagagag tctaaaagga cgttggggac aagctgtttt actaaatcta atccctacat
    47881 tgattggtat tgctactatt cttcttatta tgattcctat tatcttgttt gccaccacca
    47941 tatatgatgg atcagcgagc gtctcagaag cgacaacttc aggaggcggt agttcaggag
    48001 gcggtggcgg aattatttcc agcatcatta gcgctttgtt catgagcggt atttcttgga
    48061 cttatcttga tattttacgt ggacaaaaag atcagatcga accattcaaa gatgcatttc
    48121 gtggattcaa aggtttgttt atcggcggag tgatcagctt agctgtgctg atctcgatct
    48181 ttacaacatt atggggtttg ctattagtta ttcctggaat cattaaaagc tattcttatt
    48241 ctcaaagcta ttttatttac tacgatatcg tccaagaaac aggacaaaca cctaaagtac
    48301 tggatacgat cactgcaagt cgaaaattga tggatggtta taaaggacaa cttttctggt
    48361 tagatctttc atttatcggc tggcatatct tagcattagc aactgctggt attggttatc
    48421 tatggctcag cccatacatt tctgcaacaa aagcagcatt ttatgaacaa ttgccaaaag
    48481 atatttaaaa gagagtgtga cttgtgaccg agggggtggc aggagaacac cgtttatcga
    48541 gaggcagtaa aagaaacatt cagcccatta gcaaaaagga ggtgtgacaa cattcatgtc
    48601 acacctcctt tttgtatagt tccagtaaaa agaaaatcaa cttagaaaca gttaatcatt
    48661 cctcttcttc tgcttccggt aatcgatgga acttgatcag ttcattcacc gcgattggta
    48721 cgatagataa aatccctacc aagaaccagt gattcaaact gatgtgtact aggccgaata
    48781 gttcttgcgt aaatggcagc aaagcaacag ccgttgtaat agctaatgcc agtaacgcca
    48841 tttctaccaa agctttattg gtcgctaaat tgactttgaa gatcgattgg ctgctgcgta
    48901 cattgaacac atgcagaatc gatgaataag ctaagatcaa gaatgcaaca gtttgaccga
    48961 catgctgact tgcgtctacc catgggctga tagtatcgac ataactacct aaataataac
    49021 caaacaaggt tacgatggtg aagacccctg catttatggc aattttttgc aataaacctc
    49081 gagcaaagat cccttcattt ttcggaatgg gtggctgatc catgatgccg tgttccgctg
    49141 gctctcttcc taaagcaaat cctggaagtc catcagcaac tacattgaca aacagtagtt
    49201 gcacagctgt aaatggtgct ccccatccta acagcatcgc aatcaaaaca ataaagatct
    49261 ccgaaatatt acagcttagc aaaaagttga tagctttacg aatattctga taaaccgaac
    49321 gtccttgact gacagcgtct acgatcgtcg caaagttgtc atccgtcaaa atcatatctg
    49381 cggcactttg cgccacatct gttcctgtga tccccatggc acacccaacg ttacttgctt
    49441 tcaacgctgg tgcatcattc acaccatccc cagtcatcgc tacgactgca cccgtacgtt
    49501 gccaagattt gacgatccgg atcttgtctt ctggcgttac ccgagcatag acagagagag
    49561 ctttgacacg cgcatctaat tcttcgtctg ataattgatg cagttctgat cctgaaagtg
    49621 cttctgacgg atcattcaag atacctaatt ctttcgctat ggcactggca gtcaccacat
    49681 gatctccagt gatcatgact gttttgatgc cagccttttt cgctcgcttg attgctcctt
    49741 tactttctgg acgtggtgga tcgatcattc cgatcagacc aatcaaacgt aagtcttttt
    49801 ccagtgcttc agaagaaata tcttgcggtt cttcgtcata agtagcataa ccaacagcaa
    49861 tcactctcag tgcccgttta ccaaagcgat cattaacgat cgccgcttgt tctacatcgc
    49921 ctgaactaaa gcgcggagca agaacatcga atgctccttt ggtgattgag atgtattttt
    49981 cgcccatttg gtgaactgtt gtcatcattt ttcgttcaga atcaaaagga agctcgttta
    50041 ctcgtggata tttttcttct aattctgcct ttgtatgata attctcttca acagccctta
    50101 cgatggctac ttctgttgga ttacctttga taacaaggtc gtcatcttct tgctcgaccg
    50161 tcacatctgt acatagtgca gccattttca atatttccat cgcgctgttc gtcattgaat
    50221 cttcgatatt cgttacttca tcttctttgc tccatacccg gcggacgcgc atttcatttt
    50281 gtgtcagcgt tcctgttttg tctgagcaga taacattagc ggttcctagc gtttcaactg
    50341 ctggcaactg tcggatgatc gcatgtttcc tcgccatctt ctgcacaccg tatgcaagtg
    50401 tcaacgtaac aataacagtc aaggtttccg gtacagctgc aacagccaag gaaatcgaag
    50461 tcatgaacat ctcaaggagt ggttctcctt gcagttcacc aatcacaaat actaaagccg
    50521 cagccgctaa agcgatcata ctgattcgtt tgcctagctg attcaatcgg acctgtaaag
    50581 gtgttttttg catagtattt tcgttcaaaa gatccgcgat cttacccatt tctgtagcca
    50641 tgccaatggc agtgacgatc gctttcccac gacctgcagc aaccgtacat cctttaaata
    50701 ccatattaag ttggtcacct aatgactcat cttcttcagc aataaatgct gcatcttttt
    50761 caactgcttc actttctcca gtcaacgccg attcttctac acgtaattgt gatgcttcga
    50821 tcaaacgggc atcggcagaa atagcatcac cggactctaa aacaagaata tcacccacta
    50881 ccagttgctc tgactcaaca gtggtcactt tgccgtctcg tatgacagtt gccgtttgtt
    50941 tattcatatc ttgcaaggat tccagtgctt tttctgcgtt tccttcttgg acaatcgcca
    51001 ataccgagtt gatcacaacg atagcgatga tcagcagtcc ttcaaataaa tccccatgtt
    51061 ctgtagcaaa tgcggtataa aaggaaatca ccgctgctac aagaaggatg atcgttgtaa
    51121 aatcggacaa gcttcgaaag aattttttga tcatcgattc tttcggcgcc tcatcgaatt
    51181 tattcaatcc ctgctttttt cgccgttcat ccacttcttg tgaagacaac ccagtatctg
    51241 gatctgcttc tgttaccttc tttacttctt ctattgtctg cttgtattct tccatcctat
    51301 tcctccttta aaatcttcgt acaaaaaaag gtacttattt tactataaca tccttttttt
    51361 atttacttca aataaagggc ttatcgatta tttgttaaga aagcgtaaaa tttaaagtgc
    51421 ttatatttgc aaacgctacc aaaggatgct attatataga tgtaaggaag gaggatacca
    51481 tcaaatagac agaagaattt ctcgatagaa cagtgtaagc aaacaaagtt gttagaagta
    51541 caggtttcac ccagacatgc gtgtagagta caaacctata aatagtgaat gcgtatggat
    51601 gtttggatca cgaagtatcc gaaacaagaa gtaatgcgat acgtaacaat tgaataacgt
    51661 aaacgtctga aaattgaata ggaacaagag atgtttcaaa tgttgaagat gtaacgaaaa
    51721 caaaattttt gtttaacatg aagaaattca acaccgtgct ttgcatgtat gatggaaagt
    51781 tacaatcatt tctgtttatt tgaatccttc cgaggtacaa gaaagccgag cttatcgaga
    51841 gctcggcttt ttctttgtca taaaatcttt gtaaagcttt tttgttattc aaaactagcg
    51901 gcttttcata accttatttc acgaacatcg catctccaaa actaaagaaa cgatagcgct
    51961 cttcaatcgc atgctggtaa gcagataaag tcaattctct tcctgcaaag gcactaacta
    52021 acatgactaa agtcgatttt ggcaaatgga aattagttga gaaagcttct accactttga
    52081 attcatagcc aggcgtaatg aaaatatcag tccatccgct gtctgctttg atttctccat
    52141 taaatttcgt tccaatcgtt tctaatgttc gaattgaagt tgttccgaca gccacgattt
    52201 tcccgccatt tttacgtact tggttcaatc gctcagccgc ttcttccgtc aaacggtaga
    52261 attcactatg catatggtgt tcttcaattt tatctacgct gacaggtcgg aatgtaccta
    52321 accctacatg tagtgtcaga tagactaatt caacaccctt tgcttcgatc tcatcaagta
    52381 attctttcgt aaaatgcaat ccagctgttg gcgctgcagc agaaccattt tctttcgcat
    52441 agacagtttg atagcgatct gggtcttcca gacgttcttt gatatatgga ggcaatggca
    52501 tttctcccaa tgattcaaga atctctagaa aaattccttc atactcaaag tcaatgatcc
    52561 gtccgccgtg ttccaattct tctttcacta ctgcttgcaa tctgccatca ccaaaagaga
    52621 ttcttgtccc aactttggca cgtttcgcag gtttgatcaa tgtctcccac gtatctcctt
    52681 gtgtattcgt tagaagaagg acttctaaat gcgcaccagt atctggtttt tctccatata
    52741 aacgagcagg cagaactcgt gtattattca taactagcgc atcaccaggc tgtaactctt
    52801 caatgatttc atggaaatgt ttatcttcca tctctccagt tttccgatcc aaaacaagta
    52861 aacgagaatg atcgcggtct tttaatggtg tttgtgcgat taattcttcc ggcaagtcaa
    52921 aatcaaaatc ttctgttgtt aacatatcgt cactcacttt ctatcatgtg cttcatcatt
    52981 ctatcatgtt tttcgttctg ttggcaaaat cagcactttt tcacttctat tttttgtttt
    53041 cctaaataaa aaaactaccc tatttgtatt ttttcattgt tttaggcata ctagtaatag
    53101 atagttcttc attgaaaata gaaaaagagg tggcaccatg attgaatttc aaaatgtgtc
    53161 aaaaatttat aaaggtggaa aagtcgctgt agaggacgtc aatctttcat ttgaaaaggg
    53221 tgagttcatt tgttttatcg gtacgagcgg aagcggaaaa acgacgacga tgcggatgat
    53281 caaccgaatg actgatccaa cacaagggaa aattttgatt gatggaaaat ccatccaaga
    53341 aattgatcca gtggaattgc gacgtcagat cggctatgtt atccaaagta ttggattgat
    53401 gcctcacatg acgatccgtg aaaatatcac tcttgtccaa aaattgttga aagtcagtca
    53461 agaagaacgg aataaaacag ctgaaaaaat gattgatctt gtggaattgc cccgtgagat
    53521 gttagatcgc tacccacatg aattatctgg tggtcagcag cagcgtattg gagtcgttcg
    53581 tgcccttgca gcgaatcaag atatcatctt gatggacgag ccattcggtg cgcttgatcc
    53641 aatcactcgt gattccttgc aagaccttgt caaagattta caagagcgtt tgggaaaaac
    53701 gatcgtattt gtaacccatg acatggatga agcgattaaa ctagcaagcc gtatcgccat
    53761 catgagtgaa ggccgtgtga tccaatttga tacaccagaa aatattttac gtcatcctgc
    53821 aaacgatttc gtagaagagt tgattggaga agatcgccta ctacaagcca agccaaatgc
    53881 gacaagagtc ggcgaagtta tgctaaatac agcaatcacg attacaccgg aaaaatcatt
    53941 acaagaagca atcaaactga tgagggaaaa acgtgtagat acgttgttag ttaccgataa
    54001 ttcgaatgta ttaaaaggat ttatcgatgt agaaacgttg aataaaaaac gaggaaaagt
    54061 atccagtgtc ggcgacattt tgaataaaca tgttttttac gtaaaagaat ccgcctatct
    54121 tcgtgatacc ttgcaacgca ttttaaaacg cgggctgaaa tacgtgccgg tcgttgatga
    54181 acagaacaaa gtcgtaggta tcctgactcg tgcatcatta gtggatatcg tctacgatgt
    54241 gatttgggga gaagatcccg caactactga tgttgcagag tcagctactc ctgaagaatc
    54301 taaggaggtc tgacctatgc agcagttttt tcatcagcac ggttcagaaa ttctctcaaa
    54361 aggttttgaa catctttata tttcaggact tgctttgatc attggtatca tcattgctgt
    54421 accgcttggt gtcttactga cacgttttcc aaaagctgcc acgattgtga ttggcttaac
    54481 cagtgccttg cagacaatcc cttctcttgc tttattggca ttgatgatcc cgctatttgg
    54541 cgtaggtaaa ttacctgcga ttatcgcact ctttatttat tcattattgc cgattttacg
    54601 taatacgtac attgggatga aaaatgtcga ttggaattat cgggatgtgg caaaaggcat
    54661 ggggatgact gagttccaat ccattttttc cgttgaattg cccattgcta tgccaacgat
    54721 catggctggt attcgtttag ctgccgttta cgtgattgca tgggctacac ttgcctctta
    54781 tatcggcgct ggtggtttag gcgatttgat ttttagtgga ctgaataatt accaaccaga
    54841 tctcattttg gcaggtacga tccctgtaac aattttagca ctattggctg attggcttct
    54901 aggtctcttg gaacaccggc tgacaccgat cgcacttaga gaggagaatg aagaatgaaa
    54961 cgtatactaa aaatgaccat ggttcttttc tctcttctta tacttgcgag ttgttccttc
    55021 cctggtcttg ccagcaatac agatgaagac accatttcga tcacaggtgg aatcacttca
    55081 gaagcacaga tcttagcaag tctagttgcc ggaatggttg aacattatac agataaaaat
    55141 accgcaatca tcaacaatct ggctactaca actatcaacc atcaagcgat gatgaacgga
    55201 gatgcttcaa tttcagcggc tcgctacaca ggaacagatt taacgactac attgaacctt
    55261 cctcctgaaa aagatccgaa aaaagcgttt gctaccgtga aagatgaatt tgagaaaaga
    55321 tacggtcaaa cgtggttccc gtcctatagt tttgaaaata cgtatgtctt tcttgttcga
    55381 aaagacaccg cacaaaaata ccatctatct aaagtcagcg atctgaaaaa tgtagcggat
    55441 gaattagtag ctggtgtaga tacttcttgg atcaatcgta aaggagacgg ttatgacggc
    55501 ttccaagaaa cttatggctt ctccttcaat tcgatattac ctatgcagat tggacttgtt
    55561 tatgacgcag tcgaagcagg aaaaatggat attgttcttg gctactctac ggatggacgg
    55621 atcgccagtt atgatctagt catgttggaa gatgatcgtc aattcttccc accatatgat
    55681 gctgctccag ttgtagacaa caagatatta gctgatacgc ctcatttaaa agaagcctta
    55741 aataagttag gtcataccat cagtacggaa aaaatgcagg aattgaacta cgaagcagat
    55801 aataatctag tggaaccatc agtagtagca caacgtttct tacaagaaaa ccactatttt
    55861 gaagggaagt gaagcaattg gataaaatga atgtctttca acaatttttc tattatttcc
    55921 aggaaaatgg ctcctatatc tttgcacaat ttatccgtca cttcctgatt tctatctatg
    55981 gtgtcctctt tgcagcagtt gtcgggattc cggttgggat catgatttct aggagacgta
    56041 aattagcaaa ctgggtgatt cgtttggcga atatcatcca gacgatccct tctctagcca
    56101 tgatctccat tttaatcatt ggattaggcc tgggagtcaa tgtcgtgatt gttactgtat
    56161 ttttgtattc cctcttaccg atcatcaaga atacgtatac cgggatgatc caagtagaca
    56221 aaaacatcct tgatgtcggc aaaggcatgg ggatgactgc tagacaacga ctattcatgg
    56281 tagagttacc actctctgtt tctgttatca tggcaggcat aagaaacgcg ttagttgttg
    56341 ccatcgggat aaccgcgatc ggtgcattcg tcggtgctgg tggtcttgga gatatcatta
    56401 tccgaggaac caatgcaaca gagggcacat cgattatttt agcaggtgca ttgccaactg
    56461 cattaatggc gatcatcacg gactggcttt taggtatttt ggaaagacgg ctagatccag
    56521 ccagccgtac atcacgctga tgttattgta aaaaacaaaa gcttctgcga ccatttgttg
    56581 gtcgcaaagg cttttttagt tctgtcattt ttaaggcaat aatccactct gattcttccc
    56641 tccttatggg caaagagttg cttttcttag ctatttatcc attccgtata ctccaacttc
    56701 atactgccaa cgggttcata tctgcatcca ccgactaagt ttttttctta tccgctaagg
    56761 cattttgcca atccattttc ccatcactcg tcatggaatt cataccacat gatctctaaa
    56821 tactcgtctg cttgttcttt gaattgggca tcaattctta catcctttgg cgtggctttc
    56881 atttaagcct tttccatcag tcctcgattg ttcgatgcct tcagctgcat catcaaaact
    56941 ggtgactgcc gtatcggtaa cttcgatcat cttttataca atcgggaact tgccatcctc
    57001 atccttggct aaaaacatat ctagccattc cagcttctcg ccatgggagg tttgcggttc
    57061 ctaaaaccgg tacacgattc ttatggactg catccgtcgc aactagactt ggcgatacga
    57121 tgatccttca ccagaagagc acccccgtaa accaattgat cgatatattg ccaataagaa
    57181 aagacattgg cgtaaaagat atcgatccct cgtgatgcat tgccgttgat actgctattc
    57241 atggccgaaa gcgccacgac tttaacttct tccgtttgag tgacattcat gttcggcaat
    57301 gtcgctttgg atacccgctc tgctaaagga acgacactgg tattgtattt cgtatctgta
    57361 tcttttccaa aagaccatgc caacgaatcc tctgggaacc aataagacga ctccggttga
    57421 ttgtccattg cttttttcaa catacttttg gctgtttctc gataaacgac atttccaatt
    57481 gtcttcgcct cactttggcg ataatcgatg gtcgttgcca ctgccaacac taacaacgat
    57541 gctccaattt tactgatttt ccttgtcaca cttcttcctc cttgttttac ataaacccgt
    57601 caacattcca ttcctaaact caaacaatga cccttcaaag gtggagatat aggaacaaat
    57661 taaagaaatc atgcaataaa tccaagaagg ttggttttag aacaaaaaaa gagtctcctt
    57721 tttggggaga ctctttgtta actatctaaa aaggttggct cttctcactt cactcataat
    57781 gtgtccaggc agaataaatt tctacaactt tattttcctc atcaactttg taaaccactc
    57841 gatgctgtct attcagcctg cgtgagtatc tgccttcatg tgtaggtctc agcttttcaa
    57901 aagactgcgt gggcatatat ggatcttcct tcaatgtctg aatcacttct tcaaattgtt
    57961 tctttaaatt cgtttgcttg atttttctta aatccacttt cgcagagttt ttgattgcga
    58021 ctgtatagtt actcatcaaa gagcttccca atcaatatca tcgatattcg taaagctact
    58081 gtcgtctttt tcgcggtctc gtactttatc taaagtacct gtttgctcaa gaaaaagggt
    58141 ttcttgaatc gctctccagt cttccagccc aatcaataca gcattgtttc cactacgatc
    58201 actaataatc tcgatttcag tatgattttc atttacctct ttcaataact gataaaaatt
    58261 tttacgagca gtgctaggat tcatcgttgt attagccatt gtctctcctc ctttttctga
    58321 tataattata gcgtacttga ttaagtacgt caaaaaaata caataaagca cttaaattca
    58381 gcaaactttt cctctttttt acatagagaa aaagacccct ttttagggag gatctttgtc
    58441 cacgctcact tcttactcct ttacgactag cggtagccaa acgtaaagta attggtaaca
    58501 acgtatcatc tttagcaaag gtcgaatcca aagcctgtga gtctatgcaa caacaccaat
    58561 acacgaaggt tcctcgttgc agtgtgactt gattgatatc ttgaataacc tgaggatacg
    58621 cacgaaagat ttgtaacggt tattcaaaac tgattttggc cgtcaagtct tctactgcct
    58681 caaaaatgat tgcctctttc gtctcctgtc attcatgttc actatcgatt tgatacttgc
    58741 ttagaccgtt gggtttgtga atcaccaatt aaaaatggca ctgagttatg gtaacaaaaa
    58801 atggtcactt gacgaggctg agattcctac ttatgaagac acatggcact taagagtcga
    58861 ggtaaaggaa gaagctgaaa atataggagg ctgtacatta tttggcaaag gattcggaaa
    58921 ttatgatcaa gacatcaaaa tcaaaatgtt ttacagctaa ataggaagaa taataacatt
    58981 tcatacgctg ttcccatctt taatggctta tagagggcac ctttagctga gaataagaca
    59041 aaacagaccc tctagcttca attaagagct agagggtctg tttttgattt taaaacttgg
    59101 tttatttctt caaatggtag ctataggtgc tgacaacgat attttcacgc caagacaatc
    59161 gcaatcttaa ccgcaaactt gtttggttat ggagaatatt ctgccagcgt ctgtttggta
    59221 caaattgagg aatcaacaca gtagttgtat agttttgttt cgctgcattt ttacttacga
    59281 tatctacata acggataatt ggattttcaa tcgagcgata agaagagtgg acgattgaaa
    59341 aacggacatc tgggaaatgc tttttaaatt ctgcttcgat ttctttttct ttttcgacat
    59401 cttcgtctag cgaaacatgc atcgcaacga cataatcacc gatcgaacgt gcgtaattca
    59461 acgcaccgat atttacccgt gtcacattcc cgactaatac aagaacggta ttcccgtcat
    59521 aatcatgtag ttttgcatca ttttctaaac gaagctgttc tgctactttt ttataatgat
    59581 catggatctt ataaaaaatg aacataacga ttggcataat gatgaagaaa ggccaaatgt
    59641 cccctaaccg atagacaaac aaaatcgcaa tgattgcata tgagatgaac gcacccgtaa
    59701 tattggcaat cgatttgctt aaccatcttt ttccttcttt tttccatttg acgaccatcc
    59761 ctgtttgaga taaagcaaat ggaatgaaca cgccgataga gtataacgga atcaagcgtt
    59821 ctgttgagcc atggaaaata aacagcaata cgatcgaacc tagcgctagt gtgataatcc
    59881 cattggaata gcctagtcga tccccacgat cttgatacat gtgaggcatg aatttatctt
    59941 ttgccagatt gtatgcaaga actggaaaag cagaaaaacc ggtgttagca gcaactgcta
    60001 aaatcagcgc tgtagcaaat tgaaggacgt aatacaaaat accatgtcca aagacagctt
    60061 taccaatttg agacaaaaca gtgacttctt tttcaggaac gatcccatac caatagttga
    60121 taaatgtgat ccccacgaag aagaaaccta agatcaatgc catcatcgtc aatgtagcag
    60181 cggcattttt tgctcttggc ttcttaaaga atggtacagc attactaatc gcctccaccc
    60241 cagttaaaga ggaagaacca gaagaaaatg ctcgtaaaat caaagcgata gaaacacccg
    60301 gcacagccgc tcctggaagt gcagtagctt gcaatggcac agcacctgtc acgatattat
    60361 ataaaccaac aacgatcagt aacgagatca caaggataaa tgtgtacacc ggaaataata
    60421 ggaacgatgc agattccctc agcccacgaa gattcagcat catcagtagc aaaacgattg
    60481 tcacagaaat agcgacttga tgaccgtaga gtgctggcac cgctgatgtg atcgcttcag
    60541 cacctgctga gacagatact gctactgtca acatgtagtc gatcaataaa gagccaccgg
    60601 aaatcaagcc agcattcttt ccaagattct cactgctgac gacatatgct cccccgccat
    60661 gaggataagc atggatgatt tgacgatatg atagcgttaa tgaaatcaat aaaataataa
    60721 caaaagcggc aatgggtagc gaataccaga tggctgcagc agacaaagcg actaaaacga
    60781 caacgatttg ttccgtacca tatgcaatag atgataacgc atcagacgac aataatgcca
    60841 aagcagcaaa tcgggttaat ttgtgctcat cattttcagc agattttaaa ggcttaccta
    60901 ctaacaacct ttttaagtaa tccaatttct aaactccttt acacacaatt ttacttcaag
    60961 ttttatagtc gaaaatttat agtaaacaag atgttatagt acgctcactg accttaaaag
    61021 tcaataaact ttaagaaaat aattggaaaa aaacagaaaa atatcaagcg ctttttcaaa
    61081 cgctttaata cagcaaaaat ctaacatttt tttatgggct ttttctttga attctataat
    61141 aaaaggatag aacttgactt agaaaatcaa gttctatcct ttttgatatc ttaacttttg
    61201 gaagagagat gtaaggctgc tttctaccca tcattcttac tactcgaagc atagcgcagt
    61261 tgtcacaacc tcttgataaa cggtgtttaa aagcttgcat ctgcggtaat aagcccaacc
    61321 aaattaaaaa cataagaagc tgtttttatt tggtcgttct tattacttga tgcagagcag
    61381 cttttttaca acctcttggt acacggtgtt caatcagctg accctcggta ttagctcaaa
    61441 aaaacggcaa aatatgagga actatttttc catttttttc tctaatactc aggtcaaaac
    61501 actgatttca caacctcttt ttttaatgaa aaatatggct ctcatgcgcc gcttctttga
    61561 atttcgtcat tttttcttcg atgaacggat agattgccgt tcctacgatt ccaaagacaa
    61621 caaacaaacc gatcatgatc cagattgctt tcgtagcatt tggccaataa attcctcctg
    61681 cagattcccg taacaagttt accgcatgag taaatggcaa gaacggattg atcatttgga
    61741 agaatttccc tgagacttgg atcggatagt tcccgcctcc gccagagatc gacagtacta
    61801 agatgataat agcgatccct ttaccgacat tatcgaataa tgcagccaag acatagacga
    61861 tcatcatgaa cgctaaggcg atcaagacag caaatgacac gctgtagacc ggattttgga
    61921 catctactcc tagtaagaaa atattcccta atgtaacgat caagctttgc gcaatcgcca
    61981 ttgtcaaaaa tgtcagcata cgagcaacga attgttctcg tttcgtaaaa ttgtttcggt
    62041 ctttctttcc taaatagtat tctgtcgtag caacacttga aagaagaaca gcgccgaccc
    62101 acagacatag tgcagtataa aacggggtgc tagctgaacc gttatttgcg attggataaa
    62161 gcgcattggt ttgtagctca acaggttgtg taaagaagtc actttctttt gttgcgtcta
    62221 gtttcaataa tttgatgact tcgcctagat cgacttcttg ttcgcctttt cggatcgcgt
    62281 ctgctgcttt ttggatacca gctttgatat ttggccagtc attgttgatc aagtccgcag
    62341 cagcgttcac agctgtttcg acttctggca ttttttcatt tactgttgtc aatgtaccgg
    62401 tgatttcatt ttttagtgct ggccaatcat tttggataaa atcagcggcc aatcctaatt
    62461 tttgctgaat gatcggtaat tcattgttgt ataggtctgc cccttcattg atgccattga
    62521 cgatggtatc catatttcca ttcagcattg tattggcatc atggatttct tgtccaattg
    62581 caggcatttc tttttgatat ttttctaaaa ttgccactgc attcgttaca gttgctttcg
    62641 ttgagctgag aagcgaatca aagtcgatct ggtccgcttt gctcagtgcg ccttgtgcag
    62701 tcccaatcgt atcgatgact tggtctagta gagaagtgac agtggaactg atttgacctg
    62761 tattgatacc agcaatgatg ccattgatat tattaacaac ggcatcaatt tgatctagca
    62821 attcatcaat agccttcgtg tcttcatttt gaacagctgt gttcaaagca gataggcggt
    62881 tttgcagatt gcttagcaag ggttttaatt gatttagctg atcaataatt ggctgtaatt
    62941 tcgtatttcc agcacttgtt tgcaatttag tcaggaactc gaccagttta tcgattgcat
    63001 tttgttgttg agatattccg tcaataaagc cgttcaatac attgctgatg tttttctttt
    63061 catcatcagt cagcttacca tcttcaatag ctgttcttac agtatctatt aacgaacctg
    63121 tcgttgttgc cacttgtcca attgacttca atgtaacatc tacactgttt gtgatgcttg
    63181 gtatcgcatc tttcagctga gaggcacctg attttgtttg gtttgctaga tcacttgcct
    63241 gattttctaa atttttcaca tctggcaaga tatcttgtac ttgttggatg atcgtcaagc
    63301 cttgttttgc ttcatcgatt ccttggttca ttgtctcttg gatcgaagca aaatcgccgt
    63361 cgatctctgc tagttgctta ccggcatttt ggatctctgg gattttttct tgcaaatcaa
    63421 ggatgatttt tgcttgatct ttcaactcag gcattttgtc attcaatgca acgacttttt
    63481 cacccatctc atctacttta gggatgtaat ccacgaattc atttgcttta ttcagttttt
    63541 ccttgatttc aggtagttga ctttgtaatt cgaggacttg ttttgtgtag ccgtcgatcg
    63601 tgtccaaatt ttcatctgtt gataaaatca tatctttaac tttattgata cttactaggt
    63661 tcgtatcgat gtcgtaacct atctcattga atactttcaa taaagtcgaa ctggctgttt
    63721 tgataaattc atttgtgatt tgcgattgga tcgaacttgc tcctttatcg gtgattttcg
    63781 gtgcaatcgc attgattttt tcatttaccg tatattcgat ttttggtttc ttgatatcac
    63841 cactagtaaa actcagcaga tcttctgaga aatcctttgg caagtaaata cctgcgtaat
    63901 actttccgga acggactccg tcttctaatt gttcttttga atcaacgaat tgccatccta
    63961 attgtttatt tttatgaagc gattcgatga cttgctcccc aatggccact tctttacctt
    64021 gaaactctgc tggcttgtct gcactgtaaa cagcaattgg cagctctcca gtatttccat
    64081 atggatccca taatgctttg atgttaaacc acgcatatag cgaagggatg atcatgatcg
    64141 cgatgatcag aagtgttgca atcgggtttt tgaaaatccg tttccagtca agcaaatata
    64201 atcgaagcgt atttttgatt gactgcatag ttctccccct tcttcttttt tcagctgaat
    64261 tactttacca tgtatttcat acttttttca atcggctttt ttcaaaaaag tcgaattttt
    64321 ggacatatca cggcaattgt ctcattgtaa tctcttacat attgagaaat aattaaggat
    64381 tacagccttt actttatcat gaaataaaag ggaaagataa ttttatgcca aagtttttat
    64441 ctattatacg tatacgcaaa aaagctaaaa cattttttgt ctttgcccct acaaaaatgt
    64501 ttcagctttt ttcgtttttt attcctgtat ttcttatttt tcgaatgaca ggatgatttt
    64561 cccagcagga ggatgtcctt caactagctg atgtgcttgg atgacatgct taagtgaagc
    64621 agacagattc attgaaataa aaacatgaaa ggctcctttt tggtaggctt tgattatgcc
    64681 agcaaaggct tctgcatcta gatattcctt tcttggaaca aacgattcat aaaagccttc
    64741 ctttttttgc cgcaaatcta gatctgggag gtcatttaat gcaacatacc tgccgcctgg
    64801 tttcatgatc tggatgccgg tttctccttt gatacttcct ttcgtcgcat cgattacaag
    64861 gtctgcctga tcagcaaatt gcagacctgg atttgtttta tcataagcag caaatgctga
    64921 agcttctaat ttttttacct tttcctcatt tttactggat gcactggtta agattcggat
    64981 tcccttttca tggagaagct ggatcagact tgacccgacc gcacctgatg ctcctagtat
    65041 cattacagta tctgtcggct ggatttccgt caaatggttg attagattat aagccgtaat
    65101 tcctggcgtg accattccgg ctgcttcttc ccaactcatt ttatctggaa tttttgctac
    65161 ttttgctgat ggcaacacga cttcttctcc gtaagtcccg ccgactgcat ggacagctac
    65221 tcgatcacct acatgaacag ttgtgacatc tgaaccaatc tctgtcacaa taccagcgcc
    65281 atcattacct ggtacataag ggaatttcaa tgttcgtatc tctttcattt ctcctaaacg
    65341 caaggcaaca tcataaggat tcacactgaa tgcttttatt tctacacgaa catgtccaga
    65401 accgacttct ctgggatgag catcgatttc ttcaaaaaca tcttgtgctt ctccgtaacc
    65461 gttaattgca aatcttcgca tggtcaccat ctcccttttt ctttaagttt acgtttatgc
    65521 ctttattttg acaaaaaaaa tgtcttttcg ttttggcgca tgattcaaaa caaaatagtt
    65581 gtcttttttc gcgtaacgtg ttaaattatt tatctaaaag cgtaaagatt acgatttacg
    65641 ctaataaaaa ataattggag gaatttgttg atggctaaaa aatcaaagat tgccaaagca
    65701 aaaaaacagc aagaaacgat tgaacgctat gctgctttac gccaggaatt aaaagcgcaa
    65761 aaagattacg aaggcttgcg taaacttcct ttagatgctc gtccagaacg attgaaaaat
    65821 cgtgatttaa tcgatggacg ccctcgcgga tacatgcgta aattcgggat gtcccgtata
    65881 aaattccgtg aattagcaca taaaggtttg attccaggag taaaaaaagc cagctggtaa
    65941 tccaagtctg gctgtaatgt aaaggaggag ggcgcatgga ccactccttg gatcatcaaa
    66001 aaagatacgt tcgcttcttc tagcagaaat gctggtacta gaagcagacg tatctttttt
    66061 tacgcacagc tggtcctttt ttatatgtaa aggctgcatg gttacttcgc tatatttatt
    66121 tagtaacagg agtcagtgct tcgataccca cactaatttt caatgctcgg tcaacttctg
    66181 ccataatggg tcgatccaaa tggcagacct tttctttcaa tcttgattta tcgatcgttc
    66241 tgatctgctc caacaggata acggagtctc tttctattcc tgttccagta gctttgatcc
    66301 caatgtgagt cggaagctta ggtttagcca tttttgccgt tatggccgca acaatgatcg
    66361 tcgggctaaa atggttacct agattatttt gaataaccaa aactgggcgt gttccgcctt
    66421 gctccgatcc cacgactgga gaaagatcag caaaataaat atctcctctt tttaccatag
    66481 gatcctcctt attcttgata ttctcttggt atccgctgtc ctaatccgca agccacttca
    66541 tagtggatcg tacctaattg atctgctata tcctgcatag tgatttcttt gtctgcattt
    66601 ttgccgataa gcgtcacttt tgttccgaca ggaaattctt gaggcaatcg gatcatcagt
    66661 tgatccatac atacacgccc aatcgtttca cagtagttcc cttctaccaa tagagaaaaa
    66721 ccctgcatct ttcgtggcca gccatctgca taaccgatcg gtatcgttcc gatccactct
    66781 gcttcaggag tgatatatgt ttctccatag ccgattcctt cacctgctgg gagttttttg
    66841 acttgaatca attcagaaac cagctcaagt gctggctgca atggataaac ttccggtagt
    66901 gcgtgcccag agggattaag tccgtacatt gctactccat aacgaatcat attgccgatt
    66961 gtttcatcat gccataaagc cgttgcacta ttgctgacat gtacatatcg aggaagttcg
    67021 ggaagttttt tcagcacttc tataaagcgt tctttttgca gattccaata tgtatcatct
    67081 gcctgatctg ctgtagaaaa atgggtaaat aaaccttccc attctaatac acgactttct
    67141 ttgatgaatg cagctgcctc ttttacttct tcaggtgtac aaaatccgat acgacccatc
    67201 ccagtgtctg cttttagatg aatcgaaaga ggcgtctcta gcttactttt ctctaaaaca
    67261 gcagctgctg cttgcaacca ttcccgtgta cctgcagtta ctgataaatc atgagcaaca
    67321 gctacagtca gtgcgtcgta aggaaccata ttcaagataa ggatgggctg agtaaatcca
    67381 gcttctctca actcgactcc ttcatctaaa tttgaaacac aaaagcccga agctccacct
    67441 gcaactgctg cctcagctgt ttcaatagct ccatgaccgt atccgttcgc ttttactact
    67501 gcaaacaatt cttttccttg cggaagtctt gcaacttcat ttgctacatt ttcagttata
    67561 gcttttttat gtatcactgc tttcgttggg cggtgccaag cgacaaccat cgtttttcct
    67621 tctttctagt cttccaaaat aatttgtgca aaagctgttt catttgtatg agtaatactg
    67681 acccatacat tgccttcatg aggtgaacgc gtcacaacag gctgaccatt ttcatttttt
    67741 aaaatctcga catcttgaaa ggtaactttt ccgatcccag ttccccaagc tttagagaaa
    67801 gcctctttgc aagcgtaacg cccgcctaaa aattctactt ggcgatgaaa aggcaaggac
    67861 tggaatagtt tcatttcatc agaggtcaag attcgtgcaa tgaattttgg tttttcttcg
    67921 atcagccgag tgatcctagg aagttcgact gcatcgatcc caattccttt tatcatatgc
    67981 ttttctcttt tcaataaatt tagaacactc tcattctacc aaattataac cattttgcca
    68041 caaataagtc cacaaataac aaaaatgaaa gtttctctta aaaaagagaa actttcattt
    68101 tatgtttaat cgttatttga acggattaca aatccgcgtt ttttatcatt gtgacgttta
    68161 ccgccatctt tttggctgtt attatttttg ctataaccgc cttttgattt gtttccacga
    68221 taattaccgc cgtcacgacg attacggttg tttccgccac cacgtttgcc atttttattg
    68281 tatccttttt tctgcattgg caatgggcgt tctggtgtga tcttcactgg cactgcatca
    68341 gctggatctt ttgctgtcgt ttttagcaaa agtgcaacta aatcttgtgc agaatattct
    68401 tctaataatt tatctgctgt ttgcaggtat ttatctagac cattttctgc aagttttgtt
    68461 tcgatttgtt caactgcagc acctaattga cctttgaatg cttctttttc ggttggtgga
    68521 cgtaaagtcg tcatccgttt tttcgtcaag ttttcaatca catgcaagta gtccatttca
    68581 tttggtgtaa cgaaagtcac agacataccg ccttttccgg cacggcctgt acgtccgata
    68641 cggtgaacgt agctttctgg atcttgaggg atatcatagt tgtacacatg agtgacacct
    68701 gaaatatcta atccacgtgc agcaacgtct gtcgctacaa ggatatctaa atgaccgctc
    68761 ttaaatgaac gcaatacgct cattcgtttt tgttgagaca aatcgccatg gattccttct
    68821 gcacggtaac cgcgtgcttc taatccgcgt gccaattcgt ctacacggcg ttttgtacgt
    68881 ccgaagacga ttgttaattc aggtgtttga acatcaaaca agcgagtcat gatatcgaat
    68941 ttttcgtatt cttttgcgcg gacatagtat tgatcgatca agtcagctgt catttctttc
    69001 gctttgatct tcacatgatg tggatttttc atgaatttaa cgccaatatt cttgatagct
    69061 ggcggcatcg ttgctgagaa aagtaatgtt tgtctttgat caggaacttg tgaaatgatt
    69121 ttttcgatat cttccaagaa gcccatattc aacatttcat ctgcttcatc caaaactaat
    69181 gtttgaacgg ttccaagttt caatgtgtgg cggttaatgt ggtcaagcat acgtcctggt
    69241 gtacccacaa cgatatgtgg acgatctttc aagccacgga tctgacggcc aatatctgct
    69301 ccgccgtaaa cggcttgtac acgtactttt ttgtcgcgcc ctaaacgata taattcttct
    69361 tgtgtttgga tagctaattc acgtgttggt gcgataacta aaccttgtaa ttcgtgacga
    69421 tctggatcga ttttttctaa cattggtaat ccaaatgcag ctgttttccc tgtacctgtc
    69481 tgtgcttgtc caataacgtc ttttccagct aatgctagtg gaatcgtttc tgcttggata
    69541 ggcgttgctt cttcaaaacc agcgcgttcg actgatttca acaattctgg tgataattct
    69601 agttctttaa atttcaaatg aatcctccta tatggttgtt aactgaggat gtagcttttt
    69661 tgctaccccg tcgatttgtt cggtcaaagt gtattgtttc tttttgacag tggccacgca
    69721 cgtttttatc cattcttagc ccctgtggca ccaccgaaaa atcactttgc gaagtttagg
    69781 cattagaaaa gccaaacggg tcgcgttcag ctttatcatt attatgacat ctggcttcaa
    69841 caagttgttt ctatggcaaa accagtccaa tttctcttca tctttcggca ataggttatt
    69901 ctaacatagt ttttcgtttt ctgcaagttt acagattttt atttcatttg ctgaagttgt
    69961 tctaccactt caaataagcc catgctgttg ctgcctttta gcataatact atctgttgct
    70021 tcaagatttt gtttgatagc atcaatcaat ttattctttt ctttttcaaa atagaacaca
    70081 ggtagttggg ggtatttttg gttcaattct tcttttagtg catgcatttg ttccccataa
    70141 aggaaaatca tggcgaattt ctcaggatcg atatgttcac tcatttgcgc atgcatattt
    70201 aaagactctt tccctaattc cagcatatct gctaacacag cgattctgcg tccttttgtt
    70261 ggcagatttt taaaagtatc taataccagt cccatggctg ttggatttgc attgtagaca
    70321 tcgctcaaaa tagctgcacc atttcctgcc gttagccatt cggttctgtt ttgagtgata
    70381 gaaacatggg cgagcccttg gcgtatctca ggtatggaca gtccaaagaa gcgcccgatc
    70441 gtatacgcaa tcaaagcatt cgttacatta taacttccag gcagcgggat cgtaaatgtt
    70501 tctccttcta ctacaaaatc ggtttgtgct tttccttttg ctgtcacttc cgcatgaaca
    70561 tcgccatttc caataccgaa tgttgtgact gtttgtacta atgttgccgt taaaggtgtc
    70621 agcaacggtt catctgcggg cacaacgagt aatccgtctg atacaagtcc gtcagtgatt
    70681 tccattttgg ctttagcgat tccttctcgc gacccaagat tttctatgtg tgcttcaccg
    70741 atcattgtga ttgctgcagc ttttggttga gccataagtg aaagttccgt gatttcattg
    70801 gcgtgatcca tgcccatttc caaaacgatc ttttctgtat cttggggcat gtgcaaaatc
    70861 gtgtaaggaa gaccaatatt attgttgtaa tttccttgcg tcttatatgt tttgtatttt
    70921 tcagacaaga cagacgcggt tagatctttc gtggtggttt taccattgct tcctgtaatt
    70981 gcaacgactt ctgcgcctac tttttccaag taataggttg ccaattgctg catggctttc
    71041 aaaggatctg ctacaggaat aactggaaaa ttttttggag cttgttcaac aggcaagctc
    71101 caaaatgaag cagatgctcc gtttgtgatc gcttgctgga taaattcatg tccatctcgc
    71161 gtaccagcaa gaggaacgaa taaacttcct tcggttatct tccggctgtc aaattcgaca
    71221 ttggtaatct ttgtcagtgc agagcattcg ctgcccacgg cttgtgcaat ttcttgtatt
    71281 gttagattca ttgagatctc tcctaaaaaa ggttgtgaaa aaagcgtttg gcctaaacgg
    71341 cgggttcgca tgactctcac ttccaagtaa atttctctat tcgtatttag aagctagaat
    71401 gaacgcggtt acgcaatcac tccagcctct tctttatttc tctttattca tttttttcaa
    71461 aaaaagattg acgctgatgg tatcgattca ttcctaactg gatcagttct tcaatcaaat
    71521 caccgtattt caagcccata ttttcccata agagtgggta catactgaac tccgtaaatc
    71581 ctggcataga gtttaattca ttcaggaata attcattttt atttgtcaaa aagaaatcgc
    71641 accggctcaa tccgcttcca cctaacatcg tgtaagctaa cttcgcgtac tcttgcgctt
    71701 tttgataaac ttcttctggc acttcggctg gaatctgcat ttcgatttta ttattgatat
    71761 attttgcttc ataatcatag aatgctacgt cttttacgac ttcaccaggc aaagtcgtcc
    71821 gaacgtcttc atttcctaat acagcaactt cgatttcgcg cgcttcaatt ccttgttcaa
    71881 cgattgctcg agaatcatac tgataggctg ttgctaaagc attttgcagc tcttctcggt
    71941 tttctgcttt tgtaatgccg acactagaac ccatattcgc cggtttgaca aacatcggat
    72001 aaagcaaaga accttcacat tgatcaaata cttttttagg attttctttc cattgattct
    72061 taagtactgg tacataagga acttgcggca caccagcagc ttgtaaaata tacttggtca
    72121 tgattttatc catggcacat gcactggtca atacgcctgc gccgacataa ggcatattca
    72181 atgtctctaa gaagccttgg atcgttccat cttccccgtt tggcccatgt aaaactggaa
    72241 aaacgatggc tccttcttct ttgatttcgc ccggattgat cacttttcct gtaaagcctt
    72301 cctctgtctg tccacttggg tcccatgaaa gatgcaagac atctttgctg gcaggttttt
    72361 ctgttagtaa tggacctttg acccattgtc cttcttttgt aataaatacg agttgaactt
    72421 ggtaataatt ataataaatg gcatttaaaa ctgaaaatgc ggaaagaatg gacacttcat
    72481 gctctgcgct gcgtccgcca tatagtaaag taatcttcaa aagattgtcc tcctgtattc
    72541 taaatcgttc agtgatttac tctcatattt tagcacaaaa caaaaataaa cgaataactt
    72601 atttataaaa aaacgagcca gcttctaaaa gaacgagcgt catttatttt ttgtcagcta
    72661 gattatttgt cccgtttttt ataaaaagtt atgataaaga aaaggaggga tgatgatggc
    72721 gcaaacaaat ccacgcagcc gcttggcaga aatcattcga gtgttcatca attacaatgt
    72781 agtgccaaac tttgtacagc aaaaaaatcc cgaacaggta aaaaaagcct ttgaagaact
    72841 aggccccacc ttcatcaaga taggacagat gctttctgtt cgagaagatt tgctttcttc
    72901 tgccttcact caaacattca aaacattaca agatagtgta cctagcgaca cattttcaac
    72961 ggtcaaaaag acgatcgaaa cagaactttc tctttcatta tcagatatat tcgatgattt
    73021 ttcaaaatcg ccttttgcct ctgcttctat ggggcaggct catcgtgcca agctgaagaa
    73081 cggtgacgcg gtagtcgtta agatccaaca ccctaacatc gccgaagaga tccgacttga
    73141 tcttcaacta tttgaacgag cgatcccttt gatcaaatac attcctgaaa ctagcgtagt
    73201 tgatctaaaa ggggtactcc aagaagtaaa acgttccttg atcaatgaaa tggacttctt
    73261 aaaagaaagt caaaatggcg aacaattcta tcaaaagaac aatggctgga aagagatccg
    73321 cagccccaag atttatgacg ctttttgttc gaaaaaagtg atcgtgatgg aagaaatgtc
    73381 tggtaaaaac ttgaatcatt taatgaacgc cgaaaacaaa actgaaactt ttatcactgg
    73441 aattcaaaac aagcagctga aacaagaagt tgcaaagctt ttagttgaga actttatgaa
    73501 acaggtgttt gatgatggtt ttttccatgc tgatcctcat ccagggaatc tgctattcca
    73561 tgtactaaca aaagaagaac agacacaagc tagccgaaaa actgaaacag tccatgaaaa
    73621 agagttcggt tcctttgctt ttcgagcctc cacttctgca gaagaccctg ttgcgccata
    73681 taccatcaac tacatcgatt ttggtatgat ggggcatctt tctgcaggac ttaggcaaaa
    73741 actcacacaa gccgttttag cgctgtatac gaaagatgct tatcgaatcg aaaaagcggt
    73801 ccttcgactt tgtcagcaag aaggctcgtt tgatgaaagt cggttccacc aagaactgac
    73861 aagttttctt gaacagtatt atgattctcc tattgatgaa atcaacttgc aagaagtatt
    73921 cacacatgtc gtgacgattt gtcatcaaaa taatctgcag ttcgatcgtg atatcacgct
    73981 tttgctgaaa gcatttggga cattagaagg ggtgatccgc gtcctcgatc ctgaagtttc
    74041 cctcatggaa gtcgcttcgc cttttgctca acattatttt ctcactcatc tggatgtaga
    74101 agatacattg aaacaaagcg gattagactt attggaaggg atgaaggctg cgcctaagat
    74161 ccctcaacag ctccaccatc tattagaaat gtggacttcg ggacaaggaa aagtcaattt
    74221 ggaattgaaa aagcaagaca aattattgag caggatcgaa agtatgatca atcggcttgt
    74281 tttcggtatg attttagctg cattgatcgt agggtcctcc ctgcttgtcc aagcagcacc
    74341 tgtagaaaac gcagaggtcg tttccttatt agggatattt acgtatgcta ttgccgcatt
    74401 cgtcattatt tttttggcaa tcgatgcatt gatccaaatg tataagaaga ggaaaaagta
    74461 gaaaaaggtt gtgacagaaa gaatcagctt caagaaataa gaaggggttt tacataaatt
    74521 gtttctcaaa tttttgtgaa aattcggctt atttttcgaa gaggctactt ctgaaacacc
    74581 gagaataaaa aatgtgaaaa agctgttctg cacccagtaa taagaacaat caaaaaggga
    74641 acatggaaca catttatcct gtttccctgt tccctttttg atgtattcga gcataaataa
    74701 aataccttcg ttacctacta tcttctgtac ttggtaaacc ttagtcaatt gcctttttta
    74761 acgcttgacg aaaagacata tgatctttct ccgtaaaggg ttttggaccc tttgtcctta
    74821 tgccagcttt tcttaacttt ccactatgat aacgttgttc tagaagcgtg ccgatattcg
    74881 cttcgtcaaa ttcttttcca gagtgatgga acactcgcac acccttcggc aatacaagtg
    74941 aagctaaacc ataatcttgc gtgatcaaaa agtcattctt cttaaccata ccgacgattt
    75001 tataatccgc actatcagct ccgcggtcta cgtagacgat ttggacatga ttagcgtatt
    75061 ctttggtcgt ataatggtcc acactggtca cgataaccac gtcaagacca taggattctc
    75121 ctaattttat gacatcttct tttactggag aaccatctcc atcaatgaat agcctcattc
    75181 tttttcgcct tcttccataa atgataaaca gttactaatc cgctcagtaa aagaccaaca
    75241 ttgatccatt ttgccaagat aagcagttgt acactttcta tcggcactga ttcatggttc
    75301 agcgtgatca ataatagatg gattggctct cccatttctt ttgctaaaac aaacaaaagc
    75361 gtcatcaata aaaactggtg gatcacactc cctttatttt gagtagtata cttgacaacg
    75421 atccggcgat ccattggaat agctgtatca gtttgataat attccttatt tagccagctg
    75481 cctgttacta cgtgccagaa actaagcaga aagacaccta tcgcaaaacc atttggaatc
    75541 aagaggacca ataaagaaga actcagtcca agcaattgca tagccaatgg agaccatgtt
    75601 aaacgtttca aaatccatgg tccgactatt tttgctgcaa tcaagcctgc tgcataaggc
    75661 aaatacataa aaatcgaaag tttttctaat ccgtacagta tgccaacata aaaactgcta
    75721 aataaaaaaa ggaaattccc tagcattcca tttagaaaag taaacagatt tagccagctt
    75781 ggcaacttcc attccttctt ctggtgtcct aaaacaaaca cgaacaagat gaacagcagc
    75841 aagaaaccat aaatagcaag atcaaaaagc ctttcatcga ataacaatcg ggcatttctc
    75901 aaaagaaaca ataagccaaa gaatatcaaa aataaaacaa gctctcgcgt agctaccaga
    75961 tttcttttga tgtctttgaa atccagttcg taacgcggat aatgactaac cgtatggtag
    76021 gccatgacgt aaaaaagtgt ataaatagta aagcttgctg caatctgagt cggtgcaggc
    76081 aactcaatcg cttgatacag gagaaatagc cagatagctg caaataagta ctttttccca
    76141 tccatattga taaaaccttg cgtcttttcg tgaaaattga ccgtggtatt agcaggtaca
    76201 agccatgctg cactcagccc tagcaaaaca cttgaaaaat aaaataaagg aaaataaaat
    76261 acgcctaata ttccaagcaa tgaaccagca ccaccaatca gcagagcgat catcagcaac
    76321 gtgtaactat ctagaccaaa tcgtagactg ttgactaaaa atagtccagt catccgaaag
    76381 gcataaaaca gcgtaaatgg caaaatcgtc agccaattat gcgagtctgt tagtcccaaa
    76441 aataaaagat aaggaataaa aataaccgtg ttacttaaga taaagggcgt tgtttgtgta
    76501 aaattacgcc caaatcgttt gagtttttcg aacatgacaa tggctcctta aacagtctta
    76561 taactaatct gctcttcgat tttagcatta aaggggaaat aaaccaattt tacagaatat
    76621 attcttgtaa aaaaaaatac agaaagaagc ataagcttct ttctgcattt ttacggtact
    76681 tgattactca agcagacaag cccttctcca caaaagtctt gcccgacacc cctgcatccg
    76741 gcaggtgata aagatagaaa tcaattatcc tgtgcctcca aaatcaatta ggagctctgt
    76801 aaaccagagt ccttctcgac ccgtcgcata aatttacttt aacacagttc ctatgtttat
    76861 gcgagagctt ttttcatttt ataatcgacc atttgaaatc cttgaccgat atctgaaata
    76921 agttcttctg taatctcaaa tccttcatgc ttataaacct cgattgcttg atgattcccc
    76981 tgattcactc gcaaaaagat ctctctttcc tttgtttgcg cagcaagttc ttcataccag
    77041 cgaaaaactg aagaagtcaa tccttgacca cgtactttat ttagcaaata taatttactt
    77101 aaataaattt ttccatttat ctcttcataa gcagtgtaac cgacagtctt ttcttcgctt
    77161 tttaataaaa agtaggacac accttctgtc agttcttttt gaatctgttt ttttgactga
    77221 taagttttca gcatgtaatc cacttgtttt tgaccaatga tcggcacaaa aacttccggc
    77281 catacttctt gtatgactgc catcagctca tccagttcat tttctgttac ttttattttt
    77341 tccatacacc cacgtccttt tttacattat aaatgacctt gcgcttgatg gaataaaaag
    77401 agcatgatca tgctatacac acccaccgac cacggtttca agaaaatcac aatctgcata
    77461 aattccctaa aagataattt cgtcaaacca gctactagac agatcaagtc gtctggcgca
    77521 aagggaagca gcaaggtaac aactagtact tttttaatat ctttcgtttt tcttgtcagc
    77581 aattcatcaa atttctgata cttttttggc gataaaataa gctggacaaa ggctacgcca
    77641 tagtaacgga caagcaaaaa tccgattgct tccccaatca ctaggccaat acaactatat
    77701 aaaagcccaa atccattgcc aaacatcagc atgcccgcta ctgaggagat ccctcccggc
    77761 aaaataggaa tgatcacttg gatcacttgc aacagaacaa atatcatcac tgcatacttg
    77821 ccaaattgct ggataaacgc ttgcaaagac gtgctcgatc gaaaaatccc atgatgataa
    77881 gcataaagaa tcaacacaaa gaaaaaaaac agaccaataa tgggcatgat ctgaagacat
    77941 ttttgtaaaa ctgatccttt tttagacatc tatattcccc acatcttttt tcatttatta
    78001 aaactgcctc ctt
//
LOCUS       DACDIE010000007        57096 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.007, whole genome shotgun sequence.
ACCESSION   DACDIE010000007 DACDIE010000000
VERSION     DACDIE010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 57096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57096
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.007"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..519)
                     /locus_tag="IV250_000566"
     CDS             complement(<1..519)
                     /locus_tag="IV250_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8767875.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNVASIVMSTIFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVA"
     gene            730..1176
                     /gene="nrdI"
                     /locus_tag="IV250_000567"
     CDS             730..1176
                     /gene="nrdI"
                     /locus_tag="IV250_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAP8767876.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEYEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQRFGFPFLADYELRGTPSDVTRVYQILAEKNK"
     gene            complement(1246..3228)
                     /locus_tag="IV250_000568"
     CDS             complement(1246..3228)
                     /locus_tag="IV250_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8767877.1"
                     /translation="MAKNELTLEELAKQIYQGAGGMGNVESIVHCMTRVRMKVINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQEMVDLAGVKLGEKLPSASATTAS
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAGTWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALATGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKAVPQASA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVISSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAADK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(3242..4138)
                     /gene="murQ"
                     /locus_tag="IV250_000569"
     CDS             complement(3242..4138)
                     /gene="murQ"
                     /locus_tag="IV250_000569"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAP8767878.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELPSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISEHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEAAQAKLAEANGFVKGTL"
     gene            complement(4141..5202)
                     /locus_tag="IV250_000570"
     CDS             complement(4141..5202)
                     /locus_tag="IV250_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP8767879.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQNRLIELGSEAKKNKLELMVDISGEALKRAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPNDIDELKEAAADFSQIEAWHNYYPRLETGLDKEWYQEK
                     NQWLKTQGFLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSEHVQEQFASFQKEQTVLLHTEPVDEVFYEYILGKHTNRQDDARDVIRSADARF
                     REIPPIPVRNTAKRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(5414..5941)
                     /locus_tag="IV250_000571"
     CDS             complement(5414..5941)
                     /locus_tag="IV250_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP8767880.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQALAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVVDRLNLPMTADERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECMEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELISYE"
     gene            complement(6008..7510)
                     /gene="lsa"
                     /locus_tag="IV250_000572"
     CDS             complement(6008..7510)
                     /gene="lsa"
                     /locus_tag="IV250_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lsa family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP8767881.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQAFVYFPQQTKDKERLTYYVLNEIMDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEIAKNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKKVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDALTMNSQVSHHKRLLSVENLQLGYENLLFEPVHF
                     TIEPHQRMAISGPNGAGKSSIIHYLLGTFNGKVVGEKSQPKHLTISYVRQNYEDNRGT
                     LAEFAKKNRVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYI
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLEKI"
     gene            complement(7768..8889)
                     /locus_tag="IV250_000573"
     CDS             complement(7768..8889)
                     /locus_tag="IV250_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP8767882.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALGGTWRTIDGMDLLFRQRKISYLLTNWQGKNTAVIESAQVLGLDLLDKP
                     TDQTIRLASSYALGELIKDTLLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTRKLHLDRRNVLVSKVKVIIAADVTNPYTGQQGAAWQFGRQKGGTDTTLAFLD
                     KQAEKIAQQIKDVWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFELVSQITGLEKQI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDRELEGLFDFLPIV
                     LSIQLGPIDLEEAMEHRQTLEKTEILGQTLSRFFNILPK"
     gene            9015..9956
                     /locus_tag="IV250_000574"
     CDS             9015..9956
                     /locus_tag="IV250_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP8767883.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVVLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNKHVDLIVALTKANQLDDMFRSIQSIITENTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEAKLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAEGM
                     VRTIVSEFASIAAKEGITLDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAVWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(10099..10995)
                     /locus_tag="IV250_000575"
     CDS             complement(10099..10995)
                     /locus_tag="IV250_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAP8767884.1"
                     /translation="MNKNDVLLIAKNKSLYFFPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMDFSSSEPKTDLFFGATNNGRKERAEKYKQEFTKR
                     GVTTNYFILPLRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYQFYHSENIFLLQERDLDELPEFLHTPFQPIPSELLEFFDAENWAKR
                     FFTTDSAIYEQYEYDLSSVKNK"
     gene            11277..12617
                     /locus_tag="IV250_000576"
     CDS             11277..12617
                     /locus_tag="IV250_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8767885.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGIILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHAADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFAAYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLIIFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLTLRKGTLW
                     DKGISFFALAVLWLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            12812..13255
                     /locus_tag="IV250_000577"
     CDS             12812..13255
                     /locus_tag="IV250_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAP8767886.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINAMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILTSKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(13517..14935)
                     /gene="nhaC"
                     /locus_tag="IV250_000578"
     CDS             complement(13517..14935)
                     /gene="nhaC"
                     /locus_tag="IV250_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP8767887.1"
                     /translation="MEKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKKYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDILEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPKLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(15339..16562)
                     /locus_tag="IV250_000579"
     CDS             complement(15339..16562)
                     /locus_tag="IV250_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAP8767888.1"
                     /translation="MTTTLSTNQLKDLIQYRLPEYQALALAIHDHPEVSNYEFYSSAA
                     LIKQLEKEGFWVEKEVAGHRTGFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDEIGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDAALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAALSTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSEKGLASIAIAAELLANTALDLLREPKLLEKIKQE
                     HQEIVARKKQEGATF"
     gene            complement(16594..17433)
                     /locus_tag="IV250_000580"
     CDS             complement(16594..17433)
                     /locus_tag="IV250_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAP8767889.1"
                     /translation="MKKNIRFAVITFVAIIWLAGCSQGKAATTEKKETTVKLGIIGED
                     TDVWDDVKDRLKKENIHLEYVKFTDYNQPNAALADGSIDLNSFQHQFFLDNYNEEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENKKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDEHARPYVNIIVARKEDEDNKTYQKIVDAYQTEETAKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(17450..18130)
                     /locus_tag="IV250_000581"
     CDS             complement(17450..18130)
                     /locus_tag="IV250_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8767890.1"
                     /translation="MTEFFKTYLPNVYLIPDEFIEATKQTLYMSFWTAFIGGIIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILVLVFLSQFISN
                     QLIKKVTH"
     gene            complement(18132..19205)
                     /locus_tag="IV250_000582"
     CDS             complement(18132..19205)
                     /locus_tag="IV250_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8767891.1"
                     /translation="MIELRDISVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEAGK
                     IIEKGSSIQIFSHPEEELTKDFIRTATHLDQALETISAHAAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRQKALDYLIKS
                     GVKVTILQKNELDNRIKVIEGGN"
     gene            complement(19855..20526)
                     /locus_tag="IV250_000583"
     CDS             complement(19855..20526)
                     /locus_tag="IV250_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP8767892.1"
                     /translation="MKGAIFDLDGLLVDTERTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVQAAGSPEKVQEVRAKREEYFYQQLTSGAIQLKPYAKEILTDLKERHM
                     KLGIATTTVTKRATDILDHFQLADYFDTFTFGDEVSENKPSPVPYLTALERTGLEALS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYSVEEKEKLNLLAEGKDLRTVIEMLD
                     KKTEK"
     gene            complement(20614..23292)
                     /gene="mgtA"
                     /locus_tag="IV250_000584"
     CDS             complement(20614..23292)
                     /gene="mgtA"
                     /locus_tag="IV250_000584"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAP8767893.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASPNGLS
                     EEQIERSRETYGENAISYGKKTPFIIEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGEYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNDTLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEAAETLDTDTINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVAPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELITGEKISEMNDHELAQVAEEHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGKFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQQVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKIPFIQSNATFAMLVITTLGIIVGSVLPFTGFGAELGLM
                     PLPGNYWLWLFITVVAYLALVTAVKKIYIRRFQELL"
     gene            23952..24389
                     /gene="ndk"
                     /locus_tag="IV250_000585"
     CDS             23952..24389
                     /gene="ndk"
                     /locus_tag="IV250_000585"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAP8767894.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFATMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKGADAVPGTIR
                     GDYALPGTENIIHASDSRDAATKEIARFFPPFDTNKPSEKAAK"
     gene            complement(24411..25277)
                     /locus_tag="IV250_000586"
     CDS             complement(24411..25277)
                     /locus_tag="IV250_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8767895.1"
                     /translation="MYDGELIKKLRVNKKLTQTQLAEGICSKTSLVGIETNSVKKVSF
                     GTLKSFLERMNMTLAEYEWIRNQLDEPKKLKKKRRMLDRIQEDSFDPYKEIANNRRHY
                     KKTEDLYYLVLNLHMSKKTEVSETEFEFLRYECRIIEEYLMQVPEYGQFEVSILADFP
                     WIFSDRFIENNYLKIKKRMRRIAQLNNHEQYLFTFLTNLTLFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKSENHQEFFAVIEYTLGKNQRNSL
                     EKKLLAIAKPSS"
     gene            complement(25511..27589)
                     /locus_tag="IV250_000587"
     CDS             complement(25511..27589)
                     /locus_tag="IV250_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP8767896.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVSKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPATLGQTEGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTIL
                     YPVTGTNAEQ"
     gene            28082..29299
                     /locus_tag="IV250_000588"
     CDS             28082..29299
                     /locus_tag="IV250_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP8767897.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEENTLRSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETREKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQKKALATQMIKKAEASPCFLLYQTFP
                     GIGALTAALLLGELGDITRFKTHKQLNAFVGIDIRRYHSGKYTGQDRINKRGNPKARK
                     IIFFTIRNMIRQQRAAPNHIVDYYYKLKKQPIPKKDKVATVACMNKLLKCMHAMVRAH
                     TEYDYAYAVSVDH"
     gene            complement(29758..30381)
                     /locus_tag="IV250_000589"
     CDS             complement(29758..30381)
                     /locus_tag="IV250_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8767898.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWEEYKLTANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLVVLLLTHDGDKVARLKNGKVVTAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     ASSQKNESTELSPSDSGSGQTITGNEENQASIITNESSEGQYQTYGQ"
     gene            complement(30537..31934)
                     /locus_tag="IV250_000590"
     CDS             complement(30537..31934)
                     /locus_tag="IV250_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8767899.1"
                     /translation="MKRYQFPKNFWWGSAASGPQTEGRISGDGKGENIFDYWYKNEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HHYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFQLFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKAYHEMDQSGVI
                     GIILNLTPSYPRDESNPEDVKAARIADAFFNRSFLDPAVKGEFPSELIERIKELDLMP
                     EIHPDDLTIIKENTVDLLGINYYQPRRIKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEKGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDRNGFEA"
     gene            complement(31956..32927)
                     /locus_tag="IV250_000591"
     CDS             complement(31956..32927)
                     /locus_tag="IV250_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8767900.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMEKISEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISNRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHAIDGLIVFLSEEEWSGFEMLTKMSIPVVALNCPGGPAGIDTIVVNDR
                     ESVAKVIAHHGGQGHKTIGFIAAAQTDYVARERLAGAKEAVQKDPSIHLEIFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            complement(32934..35234)
                     /locus_tag="IV250_000592"
     CDS             complement(32934..35234)
                     /locus_tag="IV250_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 3 glycosyl hydrolase"
                     /protein_id="HAP8767901.1"
                     /translation="MYDYPYQNPKLTIRQRVEDLLSRMNIAEKVGQVNQHLYGWECYE
                     KNGDKIELTEKLKEHVKWGGGLGALYGLFRSDPWSKTDYKNGIPAKESWKVANLVQEY
                     VLQHSRWKIPVLLVEECPHGHQGLDGISYPTNIGRGNTFNVALLEQSSRLMAKELAAK
                     GVHLALVSTLDLAKDPRWGRTEECFGEDPILSARFSEAVVRGFQGEIISEKVSFLDQT
                     VEEINKKKDQIGVVLKHCIAQGEALGGHNSGTVTIGRREFSDIYGPLLKSTRNAVGVM
                     AAYNDIDGIPCHINRALFEQTLRKEIGYQGIVMADGVALDRLSDVFTDKKTAAAYALA
                     AGIDLSLWDETYTKIAEAIDNQVVDEKLLDQAVRRVLSVKFLLGLFEQPFANDPKEYW
                     DHLMEESEHLNLETAKESITLLKNDGILPLDQQVKNIAVIGPNAHDVYHLLGDYSAPQ
                     TEDRLKKKIVQEIKTEFPQSKVSYAQGCEVRNQEDQEEKLLEAVTLAQNSDVIIAVLG
                     GSSARNFDMEFLRNGAVSSKGINMDSGENVDVASLSLGGYQEQLIEQLYQLGKPIITI
                     LVQGRPYDLMKIESFTNAIATAWFPGQEGGKAIAQMISGRNNPSGRLSLSYPRNSRQL
                     PVYYYQRAASKQENYYDLSGSPFYPFGHGLSYTTFEYSQMEVKTDIDKVIISIKVKNT
                     GSVSGKTSTLVYVRLIDGVVIQRTKLLKAFRKDELRSQEERLLVFELTSEDFSYMDID
                     DKRHYAKKAVIMVEQQEKEIKCNWQE"
     gene            complement(35236..36561)
                     /locus_tag="IV250_000593"
     CDS             complement(35236..36561)
                     /locus_tag="IV250_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8767902.1"
                     /translation="MKVVDRLSEKMLPIVSKMSSQRHLLAIRDSFIVTMPLVMAASVM
                     VLLNALIFSNATVQRILDLSPLAELASIVNNGTMSILAIIVCYNIGWNLATHYIQTGV
                     IDDPSFSPVHAGILSTAVMFILMSLSSTVTLMDGNTAEATGVFLQSLTSSSGLATAMI
                     AALVSTELFIKLAKMKSFKIRMPEGVPPAVAASFNSLVPEIVVILFFSVVVFTLNHTT
                     GLNVPQLIELAIQTPLKAFVLSVPGILFLQFFSDFLWVFGMHGSSILAPIRQAPLLQS
                     IQENMDAFSVGKEIPNIITEPFTNAFGLIGGGGCILPLVIAILWASKRQEQRSIAKFG
                     LTTCLFNITEPIMFGLPVVMNPIYMIPCAIIPSINLVIAYAATSLGIISKTVAAAPWI
                     TPPVIQSFIATGGDIRAAVLTVILIILDVFLFLPFVLAANKAKLAEGGY"
     gene            36792..37217
                     /locus_tag="IV250_000594"
     CDS             36792..37217
                     /locus_tag="IV250_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8767903.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DITNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGADSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKEATVTFTKIGNYRLKDKDGNIYSL"
     gene            complement(37715..38416)
                     /locus_tag="IV250_000595"
     CDS             complement(37715..38416)
                     /locus_tag="IV250_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP8767904.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDTFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(38432..38872)
                     /locus_tag="IV250_000596"
     CDS             complement(38432..38872)
                     /locus_tag="IV250_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HAP8767905.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVLAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFEKLPVETYVGEYL"
     gene            complement(38872..39447)
                     /locus_tag="IV250_000597"
     CDS             complement(38872..39447)
                     /locus_tag="IV250_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HAP8767906.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            complement(39858..40364)
                     /locus_tag="IV250_000598"
     CDS             complement(39858..40364)
                     /locus_tag="IV250_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HAP8767907.1"
                     /translation="MDYKKRVQQMFLDSGVVLKETELERIEYTDFGLGAIETEGLNLF
                     VYENNERYCGKELVLLPYQTCPEHRHPPRDENEGKKETFRCRKGLVYLYVEGKETKKL
                     SCIIPKGQERYYTAKKEIILHAGDQYTIEQNIKHWFQAGATGAVITEFSSSSDDPSDS
                     FTNPAIYR"
     gene            complement(40375..40854)
                     /locus_tag="IV250_000599"
                     /pseudo
     CDS             complement(40375..40854)
                     /locus_tag="IV250_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(41026..41727)
                     /locus_tag="IV250_000600"
     CDS             complement(41026..41727)
                     /locus_tag="IV250_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8767908.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQEILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(41835..43277)
                     /locus_tag="IV250_000601"
     CDS             complement(41835..43277)
                     /locus_tag="IV250_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8767909.1"
                     /translation="MQSRFSEKFLWGGATAANQLEGAYDRDGKGLSVADAMPGGKQRF
                     AIIGSEEFDWTIDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPN
                     GDEGTPNEAGLEFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYE
                     RFAQTVLERYSSKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVA
                     SSKAVKIGHELRSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGE
                     YPAYTARTHKKYGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSE
                     GNLLGGVKNPFLEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDG
                     TIIDDYRIDYLRRHIEAMANAVADGVDLMGYTPWGCMDLVSASTGEMSKRYGFIYVDL
                     DDEGNGTLNRSKKKSFDWYKEVIASNGVNL"
     gene            complement(43291..45156)
                     /locus_tag="IV250_000602"
     CDS             complement(43291..45156)
                     /locus_tag="IV250_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8767910.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(45277..46128)
                     /locus_tag="IV250_000603"
     CDS             complement(45277..46128)
                     /locus_tag="IV250_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8767911.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            complement(46438..47151)
                     /locus_tag="IV250_000604"
     CDS             complement(46438..47151)
                     /locus_tag="IV250_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP8767912.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            complement(47219..47545)
                     /locus_tag="IV250_000605"
     CDS             complement(47219..47545)
                     /locus_tag="IV250_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP8767913.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            complement(47668..47895)
                     /locus_tag="IV250_000606"
     CDS             complement(47668..47895)
                     /locus_tag="IV250_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP8767914.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            complement(48217..49470)
                     /locus_tag="IV250_000607"
     CDS             complement(48217..49470)
                     /locus_tag="IV250_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP8767915.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLIGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            49531..50271
                     /locus_tag="IV250_000608"
     CDS             49531..50271
                     /locus_tag="IV250_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8767916.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            complement(50357..51115)
                     /locus_tag="IV250_000609"
     CDS             complement(50357..51115)
                     /locus_tag="IV250_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP8767917.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(51112..52140)
                     /locus_tag="IV250_000610"
                     /pseudo
     CDS             complement(51112..52140)
                     /locus_tag="IV250_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
     gene            complement(52160..55327)
                     /locus_tag="IV250_000611"
     CDS             complement(52160..55327)
                     /locus_tag="IV250_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8767918.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            complement(55551..56435)
                     /locus_tag="IV250_000612"
     CDS             complement(55551..56435)
                     /locus_tag="IV250_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP8767919.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(56530..56967)
                     /locus_tag="IV250_000613"
     CDS             complement(56530..56967)
                     /locus_tag="IV250_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8767920.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
BASE COUNT        16390 a        12194 c         9793 g        18719 t
ORIGIN      
        1 agctactaca ataaataaaa ataatggcac taatttacaa aatgtgacga tagcattgac
       61 aaagctagca ttttcaactc cacgattcac tagccatgca tatccccata agaagatcgt
      121 actcatcacg atcgatgcca cattttgacc accttcgaat acggggaaga aataacctaa
      181 tgcactcatc aacaatgttg cgaacgccac gtttcctaac cagcatgtga gccagtaacc
      241 ccaaccgctg ataaaaccag cgaaacggcc aaaaccttct tctgcgtaac taaagatccc
      301 gccttttaaa tctggacgtt ttttattgat attattgatc gataaggaga gcatcaacat
      361 tgctgttcct acgagtaccc agctgaagat agctgggcca ccagccgatc cggagatgtc
      421 cgtcataata ccaaatacgc cacctccaat cgctgaacca atcactaagg cagcaagtgg
      481 aatcaaacca attccttttt tctgttgctc tgtatccatg taagcatcct ccaaagtaaa
      541 accgctttta tcaaaaagta taacaaacct tttttatttt tacttaatta ataaaaactt
      601 taatagaaat ataattctac cgactttttt ctcattatac aagcaaagcg cctagtccct
      661 ttccaaatta caccaccaaa aaaacttaaa aagatgctat actaaaggga aagaattggg
      721 ggatgatcca tgaacatttt atatatatcg atttcaggaa atacgcgtgc ttttgccaaa
      781 catctagcag aatatgctga aaaaatgcat gcagaagatc ctgtcaatcc tgaagttact
      841 ttaaaagaaa tccatgaaaa tagtgatttt acgaaagaaa cagaaccatt ttttactttc
      901 gttcctactt atttagacgg cgggaatggg ttagacaacg gtgacacaga aatcttgacc
      961 gaaacgatgc gcgaatatct ggaatacgaa gacaatcata aactttgcct tggtgtagtc
     1021 ggtagcggta ataaaaactt caacaatcaa tattgtctga cagccaaaca atacgcacaa
     1081 agattcggct ttcctttctt ggcagattat gaattacggg gaacaccatc tgatgtgaca
     1141 cgcgtttatc aaattttggc agaaaaaaat aaataacaca aaagaagaag ctgaaaaata
     1201 aatttcggct tcttcttttt atgttctcta ttgtttctat tcttattaag ctttgacatc
     1261 catcacaggt gcttttgctt tcgctttacc tgttttttcc aagacaaagt tttttacacg
     1321 atccatattg gttaccaaga cgatcatgga ggtttctttg tctgctgctt tgattgcttc
     1381 taagtcagct tctgctacaa gcgtatcagg tgtcacttgc tgtccttctg ttactttcac
     1441 atcgaatggc gtaccgccta agtcaactgt gttgatgccc atgtgaagca agatttctaa
     1501 gccatttggt gtcgtaatgc cgatagcatg ttttgttggg aaaacagaac ttattactcc
     1561 agtaaccggg gaatagatat gtccgttttc tggttctaca aagtaacctt cacccatcat
     1621 tttttgtgca aaaactgggt cacttgcttt atctaaagaa acaactgttc cgtcagcaac
     1681 tgatgataag atagtcacac tttcagctga agcttgaggt actgcttttt caggtacttc
     1741 atttactgtg gctgtttcgg tcgctagttc ttctttttct aaacggtctt taggaatacc
     1801 gaagaagaat gtagcaacga agccaccagc gtaagcagcc aataaaccaa ggatatagcc
     1861 taaccattta ccatcagaga tcaatggaat caaagcggct cctgaaggtc caatagcgat
     1921 tgctccgaca tttccgatca ttccgatcac tgccccaccg atacctccac cgatacaagc
     1981 agtaataaat ggacgtccta gaggtaatgt tactccgtaa atcaatggtt cgccgattcc
     2041 taagattccg actggtaaag cccctttgat catttctgtt aattctttgt cttttttgca
     2101 gcggatccat aatgccaatg ctgcacccac ttgtcctgct ccggccatcg caaggattgg
     2161 aagcaataag gtacgaccag tttcatcaat catttgtaca tggattggcg tcaagatctg
     2221 atgtagtccg aacatgacca ttggcaagaa taatgtacct agaacgaagc cggcaaatgc
     2281 tcctccaaca ccaagtaccc agttgatccc accgaccatg ccgtcagaaa tgaaccctgc
     2341 taaaggcatg atgaagaaaa tttcgatcag accgatggca atcagggaaa gagttggcgt
     2401 aacgatgata tctaccgcat caggtactac tttatgcagt tttttctcaa cgattgacaa
     2461 taaccaaact gcgaagataa cacctaagat cccgccttgt ccagttgcaa gagcttcacc
     2521 ggtaaacaag tttttcagtg gtgcttctgg attcatccct gtcagcatca caacagcccc
     2581 gataactcca ccaagcgttg gcgttgcacc aaaaacttgt gcagcattga tacctgtata
     2641 gataactaga tagctaaaca tcccgttttt caagatgttc atcacatcga tatattgttg
     2701 ccatgttcct gcgtctagtg ttccagctgt gactaagttt gctaaaactg cggcaatccc
     2761 tgccacgatc ccagtgccga caaatgcagg gatcattggt acaaagatat tagagatgtc
     2821 ttttaatacc gatttgattt tagagggttt ttgtttcgat ttttgtgccg ctttcatttc
     2881 ttgtgcccgt tcgtttacac ggtcttttcc agatgcagaa gaacttgctg tagttgcact
     2941 agcagaagga agtttttctc ctaatttcac gcctgctaag tcaaccattt cctgcgctac
     3001 tttgttcact ttcccaggtc cgatgatgac ttggagctgt tcatcctcta ctacgccaag
     3061 tactcctgga atcgctttta attccgagac atttacaaga tcatcattga tgaccttcat
     3121 acgtacacga gtcatgcagt ggacaatgct ttcaacattg cccataccgc ctgctccttg
     3181 ataaatttgt tttgccagtt cttccaaagt caattcgttt tttgccatgg tcttttcccc
     3241 ctcataaggt tccttttaca aaaccgtttg cttcagctaa ttttgcttga gctgcctctt
     3301 tatcactatt tgtcaaaatc atgacgatag ccagtttgac attttgatct gcttcctcaa
     3361 agtacttttc agctgtttcg tagtcagctt ctgtcgcttg catgatgatt cgttttgagc
     3421 gttcaattag tttttcattt gttggtttga cgtcgaccat caagttattg aatactttac
     3481 cgataccgat catactgata gtagaaatca tattcaaaat cagtttttgt gctgttcctg
     3541 acttcaatcg agtagagcct gtcaaaaatt ctggtcctgc atcgacttcg attgggaaat
     3601 ccgcatgctc gctgatttct gcttgtttat tgcaagaaat cgttcctgta acagcaccaa
     3661 tcgattttgc gtaatctaat ccaccaatca catatggtgt acgaccgcta gctgcgatac
     3721 caataactat atcatttttt gttaaattca gcttgattag atcttcttgc cctaattcat
     3781 aagaatcttc ggctccttcg acggctacag tcattgcttc ttctccacca gcaattaggc
     3841 ctatgaccat ttccggttct acaccgaatg tcggtacaca ttcagcagca tctaatacac
     3901 caagtctacc actcgttcca gctcccatat aaatcaagcg accacctttt ttaaaggatt
     3961 caatcgtgtt ggcgatcact ggctcgatac ttggcagttc tttttcaaca gcagcggcca
     4021 ctttctgatc ttctttgttc atgagttgga ctgcttgaga aatactcatt tcatctaatc
     4081 caaaagtttc ttggtttctg cgttccgttg ttaggttctc taaattgatt ttgctcattt
     4141 ttatcttcct tcttttctta taaattgata gttcatacca gctttgattt ggttgatcag
     4201 cggtagttct tctttgatga ctttggctac tacattcact ttttcatccg ctggcaaatc
     4261 atatttagtt aattggatct cacccatata gcgcaaatac ttttcgttat ctaaagtaat
     4321 agatcctttc atacgcttag ctgtatttct tactgggatt ggcggaatct ctcgaaaacg
     4381 cgcatctgcg cttcgtatga catctcttgc atcatcttgc cgattcgtat gttttcctaa
     4441 tatatattcg taaaaaactt catctacagg ttccgtatgg agcaagactg tctgttcttt
     4501 ttggaaagaa gcaaattgct cctgaacgtg ttctgacaga cctgcatctc cgatataaac
     4561 aatatccgtg ttacttgcta atagatcaag agcacctgcc agaggatgga tccctcgatg
     4621 ttcttccaaa gttggtaatc cttgatacag tggacctctc aactctgtat ctcctggtac
     4681 aaagccttgt acaaggaatc cttgagtttt gagccattga tttttttcct gataccattc
     4741 tttgtccaac cctgtttcaa gacgtggata ataattatgc caagcttcaa tctgtgagaa
     4801 atcagccgct gcttctttca actcatcgat atcattaggc gtgatcgtgc tggcattcaa
     4861 actgattttc aattgatgtg accagtcggc gatctgctga ttagaaatat gataatccat
     4921 tcttaaccct gttacaccaa ttgctttcaa gggtgttaga tcttctaatg aaaagccagc
     4981 tcgtttcaat gcctctcctg aaatatcgac catcaactct agtttattct ttttcgcttc
     5041 gcttcctagt tcgatcaatc gattttgata agccgaaaca tcatcttccg gtatatgcat
     5101 ggaagtgaaa ataccgacaa agccattgga tgccatttgg cggatatact gtttcttctc
     5161 ttctgacaaa gaatcattca taaatattga aaagccaaac atattatccc tctttttctg
     5221 tatccgtctt catttctatg actagagtat atatctaaag aaacaaaatt tcaatacttt
     5281 tgttaaaata attaaatttt atttctaaaa attggattta taatgccaaa agaattgaca
     5341 cagccacaaa aaaggcactg ctaagcaaaa gcagtgcctt gaaacatttt atttaaataa
     5401 cacaaaagtc taattactca taagaaatta attctgtatt gtctaccatg aatgtaaaga
     5461 acccttcatt aggcattgga tgaaaataag cattgatcac tcttatcaag gcattatgac
     5521 agactaacaa gtagacattt tcttcgtctt ccatgcattc tttcaataaa ggaacgatcc
     5581 gtgcgtacac atctaagacc gactctccat ttgggaaacg tacagcaaac gctaaacgcg
     5641 ctttttgaaa gttttcatct ttgcttggca gtccgtcata atccccaaaa tccatttcta
     5701 ccaatcgctc atcagcggtc atcggcaaat tgagacgatc aactactgct tgtgctgtat
     5761 ctcttgctcg ttgaagaggg gaatggatga ttttcgtgat tggaacaggc aaatctacta
     5821 ccttttctgc tagtgcctgt gcctgcatct gtcctttttc agtcagcggc acatctgctc
     5881 gcccgcaaac taatccttgt gcgttccaag ttgtttctcc gtgtctcgtt acataaagca
     5941 tgtgattctc tccttcgttg acaaagttct ccttaaccat accttgttgt ctttgagatt
     6001 acaaggttta gattttctca agagaaataa tctcagtaga tactttatct agaaaggttt
     6061 gatcatgttc cactagtaac atagcaggtt tcacgttcaa aatcagttgt tctaattgtt
     6121 cttgattgaa gacatccaaa taattcaatg gttcatccca tatatagagc tcagctggtt
     6181 gggataaaga tttagccaat tccacttttt tccgttgtcc catactcatc tgttcgattt
     6241 tattatgaaa aacatctctt tccatcccaa gctttcggag attattcaaa aatgcttgat
     6301 agtctactcg gtttttcttc gcgaattccg ccaatgtccc tcgattgtct tcataatttt
     6361 ggcgtacgta actaatggtc aaatgtttgg gctgggattt ttctcctaca accttgccgt
     6421 tgaatgtccc cagaagataa tggataatgg atgactttcc cgcgccgtta ggacctgaaa
     6481 ttgccatccg ctgatgaggt tcgatcgtaa aatgaactgg ctcgaataac agattttcat
     6541 accctaattg aagattttct acgcttaaca gccgtttatg atgagacact tggctattca
     6601 tcgtcaaagc atcgatatac tcgatatctt ttaataattt ttctttctca ctgatctggg
     6661 tttccatccg attcacgatt gctttagatc gtttcatcgt ccgtgcagca tcagcgccta
     6721 ctgctccttt attcactcgc ctagattccg tatcgatgaa tccgactttt ttctttgttt
     6781 tgtccccttc tcgggaacga gaccattctg ctttttcagc tgctgttttc tttagcctgc
     6841 tgacttcttt tttcaatttt tcatttttag ctatttcaaa ttcatcacga agttttttct
     6901 gttcttcata gatagagaaa ttcccttgat aaagttccag ttggctcttt tcgattgcta
     6961 aaacatggtc cactacttca tcgataaatc cccgatcatg actcaccacg atgaatcctt
     7021 gttttttctt tttcaagtaa gccgctactt gttttctgcc agagatatcc aaatgattcg
     7081 ttggttcatc gattaacggg aaatgagtgt catccacaaa taagagtgcc agtaggactt
     7141 ttgtcttctc tcctcctgat agtgtgctga attctctcca taagatttca ggatctgttt
     7201 gcatcagttg gagctctctt tcaatttccc atatctcaaa atccataatc tcatttaaca
     7261 cataataggt caaacgctct ttatcttttg tttgctgcgg gaaatagaca aacgcttgct
     7321 gatggatcac ttgcccttga taagggagtt tgttttgcag gatattcagt aaagttgtct
     7381 ttcctcgacc gtttcgtccg ataagtccta gtttccattg tgtgtcaaag tttagattgg
     7441 cttgttcaaa taataatgtg ccttggctgt catagccgaa tgtcagattt tttatttcga
     7501 ttttagacat tattccatca cctcgtaaaa aaagaccagt cctattttca gaactggcta
     7561 aagaggatgc aagggttggg gcacacaaaa agacccatac atcgaaagaa ccaagccaat
     7621 cccgaaaatc tgcacactgc tcccttgtct ccacaaggta cagtctgaat gcagctttca
     7681 tcaatcgact taatttttca atgcacggat ccctcacttt gtcctatatt tgaaattacg
     7741 atagcataaa gaaattgttt ttgtcaacta cttcgggaga atgttgaaaa atctgctgag
     7801 tgtctgtcct aagatttctg ttttttctaa tgtttgcctg tgttccattg cttcctctaa
     7861 atcgattggt cctaattgaa tgcttaagac aatggggaga aaatcaaaga gtccttccag
     7921 ctctcggtct ctacttccgc aaatagcgat tattggtttt cgatattttt ttgcaagcac
     7981 tgctacgcca tatggaactt ttccgtggat tgtttgctga tcaatccgcc cttctcctgt
     8041 aatgatgaca tccgcttgtt cgatctgttt ttctaatcca gtgatttggc tgaccaactc
     8101 aaaaccagag accatcttcg ctcctaacaa aaaaagggct ccgccaatac cgcctgctgc
     8161 tccgcttcct gggatgtcat ctaaatccac ctcccaaaca tcttttatct gctgagcaat
     8221 cttttcagct tgtttatcca agaaagctaa cgtcgtatcc gttcctcctt tttgtcttcc
     8281 aaattgccaa gcagctcctt gttgacctgt atatgggttg gtcacgtctg cagcgatgat
     8341 cacttttact ttagaaacaa gaacgttcct tcgatccaaa tgaagttttc tagtagataa
     8401 caaaggattt ccttctgtgt acccttctaa agaagcaccc aaagattgga gtaatcctag
     8461 accaccatcg ctacatccgc ttcctcctaa agtgacaatg atttgttcca cttcttgaag
     8521 cagtgtatct ttgataagct ctcccaaggc ataactagaa gcaagtcgga tggtttgatc
     8581 cgttggttta tcaagtaaat ctaatcctaa tacttgtgca gattcaatga ctgcggtatt
     8641 ttttccttgc cagttcgtca ataaataact gatttttcgc tgacgaaata agagatccat
     8701 cccatcaatc gtacgccagg ttcccccgag cgcgtaatag attgcttctg cagtattttc
     8761 tccgccatct gcaatcggca cattgatttt tcttccttta cggtcttccc aaccatttag
     8821 aactgcctga tttaattcaa atgaagaagc acttccttta aatgaatcga ttgccgccaa
     8881 aatatccatc tatatccctc actttcttct taagtaagac actttttttc acttttttca
     8941 aacaaaacaa tgctattttc ttctgttttt ctatatacta aaagggaact cattgaataa
     9001 aggagagaaa aaacatgaaa atcgcaatag ctggagccgg agcaatgggc agccgaatcg
     9061 gattaatgtt gcatcaaagc ggaaatgagg tcgtcttgat cgatcgctgg cctgctcaca
     9121 ttgaagccat tcgtacaaat ggattgattg ctgactttaa tggcaaagaa gtagttgcaa
     9181 aactgccaat ctattcacca gaagaaatca tcgaaagcaa caaacacgtg gatttgatcg
     9241 tagccttgac aaaagcgaat caattagatg atatgtttcg ttcgatccaa tccattatta
     9301 ctgagaatac ttatgttttg tgtttactga atggattagg tcatgaagat gtactggaaa
     9361 aatatgtacc aaagaaaaat atcttatttg ggatcacgat gtggacagct ggtctagctg
     9421 gtcccggaaa agtcacatta ttaggtgatg gagaaatcga actagagaac ttggaaccag
     9481 aaggcgaagc atttacaaaa aaagttgtag aagtctttca agaagcaaaa ttgaatccaa
     9541 tctatagtca taatgttcgt tattccattt ggcgaaaagc ttgtgtaaac ggaacattaa
     9601 atggattatg cacgatattg gattgtaata ttgctgaatt aggtgcgcaa aaagcggcag
     9661 agggcatggt tcgaacgatt gtatctgagt ttgcttctat cgcagcaaaa gaaggaatca
     9721 cattagatca agaagaagtg taccaacata ttgcttctac ttatgatcct gacaacatcg
     9781 ggttgcatta cccatctatg taccaagacc tgatcaaaaa ccatcgtttg actgaaatcg
     9841 actacatcaa tggtgccgtt tggcgaaaag gacaaaaata tgatattgct acaccatatt
     9901 gtgccttttt gacccaatta gtccatgcaa aagaaggaat ccttggcgca gaataaaagg
     9961 ctctctgatg agaatcagct ggatcagcag ctggttctca tttttttacc cccatgaaag
    10021 ttcaaaaaga aaaacacaat tctcatgttg ccataagaat tgcgcttgtt tattttcatc
    10081 tatgattggt tggcactttt atttattttt aacagaggaa agatcgtatt catattgttc
    10141 ataaatagcg gagtcagtag tgaaaaaacg ttttgcccaa ttttctgcat caaaaaattc
    10201 caacagttct gatgggattg gttgaaatgg cgtatgcaaa aattccggta attcatctaa
    10261 atcacgttct tgaagcaaaa aaatattttc agaatgataa aactgataat gagctatggc
    10321 ttgattatct gtcactagtt tcttttgaaa atacatagat tcaaacgttc gcaaagtcac
    10381 tccttgctgc ccttcccgca acagttctag aatccccctc gattccccgg tttttttcag
    10441 atattcttca taagtaaggt atccaggttg ttcatttcct cgaagcggaa gaataaagta
    10501 attggtcgta acacctcttt ttgtaaattc ctgcttatat ttttctgccc gttcttttct
    10561 gccgttattt gttgcaccaa aaaatagatc cgtctttggt tcgctagaag aaaaatccat
    10621 atgtttcgaa taaaaggaac tattatgttt tagtccaagt tctttcgctt ctaaaaaatc
    10681 aaaatgataa atctcatcga ttgccggttc atcttgtgcc tgcttcagca tcgcctcgtg
    10741 ttctgcttta aagtggttcc aaaaaaataa cacgattttt gcattggtaa atggacgcac
    10801 gcgtcgagcc atctccatat caaatccata atctaaaaaa acgatcagct gataatttgc
    10861 caactgttgt cgagctgatt taatgaattc cgactgtagt aaatcttttc cggaccaaac
    10921 atctaaagag cggatggaag gaaaaaaata aagtgattta tttttcgcaa ttagcaatac
    10981 gtcgtttttg ttcatctgtt ccgcctcact ttctctgtca ctttttccta tgatagcctg
    11041 tttaaatagt aaaaagccag taaaacgctt cctattattt ttataaaaat ttaacatttc
    11101 tatgaaaata cgatgaaata aatcattctt ccattaaaat atgcccaaaa gttttctgaa
    11161 tagttgtaat tatctgaaaa tttgttatca ttagtcatta ttattattac tatttattct
    11221 tactatttat tcttactatg cattttgatt gtgaatttaa aagaaagagg ttatttatgg
    11281 aagataaaaa attgaaaaaa caacttagct cccgacatat caccatgcta gctttaggtg
    11341 gagcgattgg tgcaggtcta tttaaaggaa gtggcgaagc gattggaatc gccggccctt
    11401 ctgtattgat cgcttttttg attggcggca taattttata tatcgtgatg aaaggtctgg
    11461 gaaaaattgt tttaagtggt ggtgatacgc accacggatt atctggattg attcgtccat
    11521 atctaggtgc tcatgctgcc gattttaccg attgggtcta ctggtccatg tggattatca
    11581 atatcattgc tgaagctgtt gcagctgctt cctttttaca gttgtggttc ccccacatcc
    11641 cagcttgggt atttgttttt atgttggctg ttttaactac tatcatcaat ctttattcgg
    11701 tacgattatt cgcagagaca gaatattggc tggcctttgc taaaatcagt gtcattatcc
    11761 tattgatcat ttttgctgct tatcttgtcg gtcagcaaat gttgggtact ggtgttttcc
    11821 ctacccttca acaattaact gatcatggtg gttttactcc ccacggtatg aagggaatca
    11881 tcagttcttt gcttgtagtt atctattctt acggcggttc tgagttgatt gctatcacag
    11941 taagcgaagc agatgatcct aaacgtgcga ttcctaaagc aatcaaaggc gtcatgggaa
    12001 gaatcatttc cttttacatt attcctttat ttttgctttt gattattttt ccttggaata
    12061 ctttagcagg cacgaacgtt agtccttttg tgatggtatt tgaaaaaatg aatattccat
    12121 ttgcggcaga tatcgtcaat ttcgtgattg ttcttgctct gttctcgtct atcaattcag
    12181 gtgtatatgc ttcttcgaga attttgttct tccgcttgaa agatcggaaa ggttcctcta
    12241 gaaaactagc tgttttaaac aagcatcaag taccgcaacg tgctgtgctg ttttgtacca
    12301 gtgttcttta tctaggtgtg gctctttctt attttgtcgg tgacaaatta tttggctatc
    12361 tggcaggttc tctttcctat acagtattat tgatctggat cattatcagt gctgcgtcct
    12421 ttgtactgac actaaggaaa ggaacgttat gggacaaagg aatcagcttt tttgcattag
    12481 cagtcttatg gttgattttc cttggtatcc tattcacgaa ctccattggg gtaactgtat
    12541 tgaccggttt gctttacctg ttcatttatt tcagttatca aaaaaagaat gatgctttcg
    12601 tcttgacgaa tgaatgaaac caatagagaa ttttattcta tttatattga atatctggag
    12661 ctattgaaaa aaaggagctg cgacaaaaat tgttgcagtt cttttttccg aataaacggt
    12721 gttcaaaaac catctgtgca gattaattga ttgttctcca cgctcatgac atgatagaag
    12781 aaaggaatga agaaaaagga gtttgtttta aatgaaaaag tattggaagt ttttgttgat
    12841 gattggtgtt tcgacggtag tcatgtttgg tatgatgtat ttgaatgttt atgcgcttga
    12901 tcatatcttc ttcagtcgca cacgtgtatt catggcgttg atgatgggtg cagtcatggc
    12961 agtcatcatg ttgttgttca tgtggaaaat gtatgaaaac aaaaagataa atgctatgat
    13021 tttagccgct agtcttcttg tttttgccgg ttccttattt atggtacgaa gccaaacaac
    13081 tgtcaacgat acggcgtgga tgaaggcgat gatccctcat cactctattg ctatcttgac
    13141 aagtaaacga gctgatttaa aagatcctga agtaaaaaaa ttagcggaag aaatcattca
    13201 ggcacaagaa gaagaaatcg acaaaatgaa aaaaatgatc aaagaaatgg aatgaccatt
    13261 atccatccaa ataaaaaaag aggatgttcc agaagtgatc aacttcaaga aataagaaga
    13321 agctacttct ggaacaccgt gaatcagagg ttgtgaaaaa gctgccctgc atcaagtaat
    13381 aagaacagcc aaacaaaaat agcttctcat atttttcgtt tggttaggct tattaccgca
    13441 aatgcaagct tttgaacacc gtttattcgt aaaagggagt gtgacaaaag tcttgtcaca
    13501 ctcccttttt acgtttttat tgttctaatt gcgctgtctt gcttactgct tcttgcggtt
    13561 tcacaaattt ctttgagatc ccccaacctg taaaggcact gaccatcgaa aacacaggtg
    13621 aaagcaaact gaaaaacacg aatggcaagt aagtgagtgt aggaatgcca aatgtacttg
    13681 ctacgaaact tccggcgatt ccccaaggca ctaagtagtt gattactgtt ccaccatctt
    13741 ctagtgttct ccctaataca gttggatcaa atcctgcttt ttcgtaagtt tctttaaatg
    13801 cattaccagg caggataaca gatagaaatt gttctccaat aaagatattt actccgatgc
    13861 ttgtcatcaa tgttgtaaaa atcaattttg gggtgttcgt catctttctt gatactcgtt
    13921 ccataacggt tgagatcaac ccaaattcga tcaacaaacc acctaatgac aatgttagaa
    13981 taatcaatga cacagttggc atcatgcttt cgattcctcc acggctaagt agtgaatcga
    14041 tttgttggtt tccagtattt gaaacaaatc ccgattcgat gacccctgcc aacttcgtta
    14101 aggaagtttg tgggttttga ataaagatca ttcctactgc tacagcaata ttcaacaaaa
    14161 tcgtcaaaat tgccggtacc tttttccatg cacacaagag cattaaaaat agaggaatca
    14221 gtgaccagat cgaaatagta aagtttgctt ctaaaatatc agtcacttca cgaatagatg
    14281 aaagacttgc ggctttactt gtgtgtccta aaatcatgaa taaaaagaaa gaaacaacaa
    14341 atgccggaac agttgaccac atcatatgct tgatgtgttt gaacaaatcg gcatcaacaa
    14401 tagctgctgc taaatttgtt gattcggata atggagacat cttgtctcca aaaacggcac
    14461 ctgaaacaat ggctccaaca acaagggctg gatttagtcc tagtgttgtc ccaataccga
    14521 aaaatgcaat cccaatcgtt gacataacag taaaggcact ccctacagcg ctacctacga
    14581 ttgcacaaac aacaaataca gaagggacga accactggac actaatcaat ttaaatccaa
    14641 tgaccatgag ggttggaata attcctgctt gtatccatac agcaatcaat gaaccgacta
    14701 aaataaaaat aaagattgga atgatccccg gtttgatgcc attgatgatc ccttcgtgca
    14761 gccaatctgt cgggtatttt tttatcactg cgtacaacat gaccattccg atagctgcca
    14821 aaatcgtgat attaggtgaa acctttaatc cgacaacgcc aatcgcaatg cctaaaacaa
    14881 tgaccatcaa tacaagtgtt gcttctaaaa aacttggtgt tccttttttt tccatgataa
    14941 tctctccttt tttagttgtt cttatgttct aaaaaagtta agatatcgga ttactccgcc
    15001 acaattcatc cgcgattcct cctttataaa aataaaaaga cctgctttca actaaggacg
    15061 aatgactccg cggtaccacc ttaattaaaa acagatctgt ttttcacttt caaacgatta
    15121 ttcaaatgta tggactttcc atgtaattcg tcaattaggc ttgatcggtt cacagcgacc
    15181 accgactttc tgaacaaaac acctaaccac tactgcgtga aaagttgact tgtatgaagt
    15241 ttaccttcca taagatcatt tgtcaatgaa aaagaaaata ttttttcatt tttctgaaaa
    15301 ttatttttat aactttcgcg cacaacttta tctttttact agaaagtagc gccctcttgt
    15361 ttttttcttg ctactatttc ttgatgttct tgtttgattt tttctaatag cttgggttct
    15421 ctcaataaat ctagagctgt attggcaagt aattccgctg caatcgcaat agaagcaagt
    15481 cctttttcac ttttagcagc agctttgaat tcttctgtat gtccggcaat atatgtctca
    15541 gagattgcta cagtaggctg tatcgttgga ataacttggc taacatttcc tacatcagaa
    15601 gaacctagac ttgttctttg ttccgtttct atttcttcat caggaacccc tgcttctttt
    15661 gcatgttgga agaataagtc atcgaaagaa ggcgttacga tgacatcatc gactgcattt
    15721 tggaacaatc caaactcaaa agtcgtaccc gtgcttaatg cagcacctcg tacgatattt
    15781 tccacttttt catatacttt gtctaatgtt tgtcgatttc ctgcacgtaa atagaaacgt
    15841 ccagcagcgt agtctggtat gacattggcc gccacgcctc catctgtaat gattccatgg
    15901 atattgacat cttttggcaa ttgcaagcgt agagcgttga ttccgttaaa cacttggatc
    15961 aaggcttcca aagcatttac tcctttttca ggtgctgctg ctgcatgaga agcgacaccg
    16021 taaaatttga tatcgactgg atcatttgct aaagattctg tcgttttccc ataacgaaaa
    16081 gctggatgga cacataatgc tgcatcgaca tcctcaaaaa aaccttcccg aacgaagctt
    16141 cccttagctg atccattttc gcctccttct tcccccggag taccatacac acgaatttct
    16201 ccgccaatct catcaatgaa ttgtttcact atgctggctg ctaatacgga gtaagtacca
    16261 aataaattat gtccgcatgc atgaccaata cctggtaaag catcaaattc tgccaaaaag
    16321 gcaattgtcg gacctggttt attggaaata taacgtgcat caaagcctgt tcggtgccca
    16381 gctacttctt tttctaccca gaacccttct ttttccaatt gtttgattaa agctgctgag
    16441 gaataaaatt cataattact tacttcagga tggtcgtgga tagctagtgc aagtgcttga
    16501 tattctggta aacggtattg aatcagatcc ttcaattgat ttgttgagag tgtggttgtc
    16561 ataaaaattt cctcctatgc tagtcgtttt ttcttatttt ctcccgaatg tttcccacgc
    16621 aggtacgctt gaaccttttg atgttttttc gattactttg gctgtttctt ctgtttgata
    16681 ggcatctact attttttgat acgttttatt gtcttcatct tcttttcttg caacgatgat
    16741 attgacataa ggacgtgcat gctcatcgac tggttctaag aagattgcat cagaagcggg
    16801 attaaatcca gcatctacag ctacaccgct attgatgact gaaatatcta catctgacaa
    16861 agcacgtgct gtttgggaag catctaactc agaaatgtct agattttttt tattctcggt
    16921 aatatcagaa acggtaggcg cttggccttt tgccttgtcc actttgatca atccagcagt
    16981 ttgcaataac agaagagctc tgccgccatt cgtgacatca ttagggattg caacttttgc
    17041 tccatctttg atatcagcca catcttttaa cgtattggaa taaattccca acggtgcatt
    17101 aaccgtattc ccaatcgata ctaaatcagt tccgtgttct tcattgtaat tgtctaaaaa
    17161 gaattgatgt tggaaggaat tcaaatcgat cgatccatct gctaaagccg catttggctg
    17221 attgtaatct gtaaatttca catattccaa atggatgttt tcctttttca aacggtcttt
    17281 cacatcgtcc catacatctg tatcttcccc aatgatccct agttttacgg tcgtttcttt
    17341 tttttcagtt gtagctgctt ttccttggct gcatcctgcc aaccaaataa ttgctacaaa
    17401 tgtaatgact gcaaaacgaa tatttttttt cattttttct cctccatttt caatgtgtga
    17461 cttttttaat cagttgatta ctgataaatt gactcaaaaa aactaagacc agaatcaata
    17521 atgtagcgac aatcgttaca tctgtttgga atcgattata tcctctcgat atagcaagat
    17581 tccctaatcc tccggcacct actgctccag ccatggtagt cagtccgatc acattgatga
    17641 tcgtcaacgc tgagacccga atgataccag gtaacccttc taacaaataa acacgtaaaa
    17701 tgatgccgat tgggctcgtg cccatcgctt ctgctgcttc gatcacccca ggatcaactt
    17761 ctagcagtgc attctcgatt tgacgggcaa aaaacgggac aacgccaaaa atcaaaggga
    17821 ctaaagctgc tttggcacca atcgtcgttc caactaagaa acgagtagtc aaagctaaaa
    17881 gagaaagcaa gataatgaaa gggatggaac gaatgatatt gatcaactta tcaagtattt
    17941 cgaacagcag cctgttagct agtaatccat ttggacgtgt cactactaac gtcacaccta
    18001 agatgattcc gatgatccca ccaatgaatg ccgtccaaaa agacatatat agtgtttgct
    18061 ttgtcgcttc gatgaattca tccggaatca aatagacatt cggcaaatat gttttaaaaa
    18121 attcagtcat tttagtttcc tccttcgatg actttgatac ggttatctaa ttcatttttt
    18181 tgtaaaatag ttacttttac accagattta atcaaataat ccaatgcttt ttgccgctgt
    18241 ttggtttctc cagctaaagt aacgatcaaa cttcctaagg gtgtttcttg taatagctct
    18301 acattaccgt ataagatatt cgctgttact tggtacttgc tgtaaagatg agcgattaga
    18361 ggttcgttcg tctggttgcc gatataactt aattccacca accatttatt tgcaatctga
    18421 tcggcaaaag cagcatgggc actgattgtt tccaatgctt ggtcaaggtg tgtcgctgtc
    18481 cgaataaaat ctttagtcaa ttcttcttca ggatgactga aaatctggat gctgctacct
    18541 ttttcaatga ttttccccgc ttccataacc gctaccttgt tgcagatttc tttgacaact
    18601 tgcatctcat gagtgatcag gacgattgtt agacctagct gacgatttag tttttttaac
    18661 agtgcaagga tctgaagggt cgttttcgga tcaagtgcac tagttgcttc atcgcataac
    18721 aacactttag gttcatttgc cagagctcgt gcgattgcca ctcgttgttt ttgccccccc
    18781 gacagttgtt taggatacgc agatgctttt tcttccagac cgacaagttc cagtagttca
    18841 ttcacttttt gacgacgttc ctgttttgat tttccagaat actttagaga aaaatctacg
    18901 ttgtcaaaaa tcgttcgact ttccattaga ttgaaatgct gaaagatcat accgatggcc
    18961 tttctttctt ttcgcaattc ttttggagag agctggctta aattcgtctg attgacgatg
    19021 acttctcctg ctgttggttt ctgcaacaaa ttcatcacgc ggactaacgt actctttcct
    19081 gcacctgagt aaccgacgat cccataaaca tccccttttt caatcgttaa atctacagaa
    19141 tctactgcct gtatctcttt ttctttttgt ttgaaacata cagaaatatc tcgcaattca
    19201 atcattaaca ttcctccttg tctataaaaa aagcatcctc aagaaaatga aaatagtcat
    19261 tttcttgagg acgccttttt gcgtgttacc acctcatgtt caagctgtgt tcacacacaa
    19321 ctcctcttca agtaccatca tactctagca agatatcggt cgcagccgtc accgccttac
    19381 atctatgctc gggatgaatt gctcgatggc cattttccaa tagtcgttcc ttgcttcttc
    19441 tcagctaccg aagctctctg tcaaaggttc ttctatcgta cttaccattt caatgcaaat
    19501 aatattcaaa aaaaaaatcc ttttactaac ataaactatg tcagtaaaag gacgacgtat
    19561 cgtggtacca ccttttttcg gaagtttcct tccctcaagc ccaacatctc aaaaagagag
    19621 attggcgttc gttttaacgg tcgaacattt ccgaatttgc ctacttatcg acaaattatt
    19681 ctccaaggcc attttcagct tttcttactt gactcttcac accagccaga gcctctctgc
    19741 aaagtttcca agcttacttt ccttctcaac gaaatcaata ttttgttgtt gggaataatc
    19801 tacaggagga aaactgaaaa gtcaagcatg aaatccaaat tattttttct atatttattt
    19861 ttctgttttt ttatctaaca tttcaataac agttcgaaga tcttttcctt ccgcaagaag
    19921 gttcaatttt tccttctctt cgacgctata atttcgaaca aaactggaat cgggaatcaa
    19981 aacaacgcct agtcctgcac gtgaagcggc tgtcgctcct actaaagaat cttccactgc
    20041 aaaagctgaa agagcttcta atcctgttcg ttccaaagct gtcagataag ggactggtga
    20101 aggcttgttc tccgatactt catctccgaa cgtaaacgta tcaaagtaat cggctaattg
    20161 aaagtgatcc aagatatctg ttgctctttt cgtaacagtt gtcgtagcaa tccctagttt
    20221 catatgacgt tcttttaagt ctgtcaatat ttcttttgca tagggtttca attggatagc
    20281 tccacttgtc agctgttgat aaaaatattc ttcacgcttt gctcggactt cctgcacttt
    20341 ttcaggactg cctgctgcct gcactaaaat atcaaaactt tgtttccagt tttttccgcc
    20401 ccaagcgtca acgatttctt tcgggatatg aatatcaaat tgctggaatc cccaaagcca
    20461 accatcccga tacgttcttt ccgtatccac taacaaacca tctaaatcaa atattgcacc
    20521 tttcatctat acattaatcc tttccaaaac aatacaccta gtttagcatg actgatccaa
    20581 aatgaacaga agcatagagc ccagccgcat cacttataag agttcttgga atcgacggat
    20641 atagattttc ttcacagctg tcacaagcgc cagataagcg acgactgtta taaacaacca
    20701 taaccagtaa ttgccaggga gcggcatcaa gcctaactca gcaccaaatc ctgtgaatgg
    20761 aaggactgaa ccgacaatga ttcctaatgt agtgataacc agcatggcaa atgttgcatt
    20821 gctttggata aatggtattt tcggtgtacg caatgcatgg agtactaatg tttgtgtcca
    20881 cagggattcg acaaaccatc ctgcatggaa taatgcgata aaaatcactt gttgtgagct
    20941 atccaatgta tggaaacttc caccaactac agcaggacaa ataacaaaat acatcaacaa
    21001 gtaagtagtg atatcaaaaa tcgaacttgt tggaccaaaa taaaccatga attttccaat
    21061 actagatgca ttccatttct ttggctcatc caaatattct ttatccatcc gatcccatgg
    21121 taaggaaatg caagaaacgt catagatcag attcaaaaat agaagttgca aaggcaacat
    21181 tggtaaaaat ggtaagaacg tactggcaat cagtacggaa aacatattgc caaaattaga
    21241 acttgctgtt gccttgatat atttcatgat gtttccaaaa gtttctcttc cagaaagaag
    21301 gcctctttcc aaaatcgtca aatctttttc taaaaggatc acgtctgctg attcttttgc
    21361 gatatccaca gctgtatcca cggaaatacc gatatcagcc actttcattg ctggagcatc
    21421 attgatacca tctcctaaaa atccgacagt atgcccattt ttacgtaaag ctgtcgtcaa
    21481 tcgtgctttt tgctgaggat tcaattttac gaatacttcg tgttcttcag ctacttgcgc
    21541 taattcatga tcattcattt ccgagatttt ctcaccagta atcaattcat tgatttctag
    21601 gcctacttga cggcacacag accgtgtgac taattcattg tccccagtca gaacttttac
    21661 agctacacca tgttctttta aagctttcaa cgcttgtttt gttgtttctt ttggtggatc
    21721 aaggaaagca agataaccaa tcagaaccat gtctgactca tctttgactg aaaattctcc
    21781 aacaacgctt ggattggtct tttgtgcgac accaatcact cgtaagccgt cttcattaag
    21841 tgattgtact tttgcaagca ccgcttcttt tcgttctttt gttaatggtg cgacagtacc
    21901 accaagatca ataaatttag aaatgttcaa catttcttca atcgcgcctt ttgtgatcat
    21961 ctgtgttttg ccagccggat cagcgactac aacactcata cggcgacgtt caaaatcaaa
    22021 aggaatttca tctacttttt gataattgat tgtatctgta tctaaagttt ctgctgcttc
    22081 atcaatgata gccacatcta acaaattttt cagccctgtt tgatagtagc tgtttagata
    22141 agcatggcgc aaaacacgat catcttcatt tccttcacaa tccatatgat attcaagaat
    22201 gattttgtct tgtgtcaatg ttcccgtttt atccgtacat aacacatcga tcgcaccaaa
    22261 attctggata gcatttaaat ttttgatgac tgttcctttt ttggccatca cacttgctcc
    22321 tttgaccaaa ttcgtcgtaa cgatcattgg aagcatttca ggcgttaaac caactgccac
    22381 agataatcca aaaagaaaag cttctaacca atctcctttt gtaagcccat tgataacgat
    22441 aactgctgga gccatcaatg acataaattt gatcagcagc atggatgttt tgtggatgcc
    22501 taattcaaaa cttgattgga tgggtttttc agatagcgtt tctgctacat gcccaaatag
    22561 tgtgtcattt ccgacagaaa caatgattcc ttccgcgctt ccactgatga cattgcttcc
    22621 cataaagaca agattttcat aacttgtttc actttgctga tcgtcttcac atactttcgc
    22681 atgcttttct actggatagc tttcccctgt caatgctgct tgagaaatga atagatcctt
    22741 tgcggacaat aaacgcatat ccgctggtat catatctcct gctgaaagcc gaacaagatc
    22801 tccacagacg atttcttcca taggaatctc tgtatactcg ccatttcgtt tgacagctgc
    22861 cgttactttc acaagtgatt tcagtttttc agctgcttga ttagaacgca cagactggat
    22921 caacgtcatc gtcccactga cgataaccat agtgaagata atggctacac caaagagatc
    22981 tttatctcct ggagcggcta atacgtattc cgtaatgaat gaaataattc ctaatgcaat
    23041 caacactaat gtaaagggtg tgatgtatgc tttcaatatt tctataatga atggtgtttt
    23101 tttgccatat gaaatcgcat tttcaccata agtttctctt gaacgctcga tttgttcttc
    23161 tgacaatcca tttggtgatg catggaacaa ttcaaataat tcttgcttcg tttttccagc
    23221 aaaagctgca tagatttttt gtgtatcttt ctttttttcc atctcgatct ctcctttgtg
    23281 tccaaaagac agctgttctc gctgtttttg gtcttttgct attcatcgga tcgatagaga
    23341 aaatgctgaa aggggctgag aacagataga taatagccct ggcctttcct ctaaatcctt
    23401 gtttttcgaa tgactgtcat ccatttgttc tcatctcctt tactaagtaa ttttaattca
    23461 tgacttccaa tcatttttcg aatagttatt ttcccaactc gctcacctcc ctttttgttt
    23521 tttcacctat gggatttgcg gcaacaaaaa aacctctcgc caaatactca gcgaaaggtt
    23581 tgaaaaagca aatcaaaaaa agcttctcag atctcatcgt tgagttttga cactgtacaa
    23641 cgtaaaaagc agatatctgc ttttagctac cttatgaaaa gccttatctc gacaattcct
    23701 gttctaccca ttggcgtctc tcgacgtttt tgggcagtag cctatgtttg tataggagcc
    23761 tcacctaaca aatcctattc ggttttccct cgttaatata acaactaaaa aagaggatgt
    23821 caaatcaatt ttccatgttt ttcatacttt tttaaaatac acgcgttata tttcctgtct
    23881 cacgcagttt tatttgtctc attttccacc tcccgctata atagaattat agtaggaaac
    23941 gaggtgctgt tatggaacga acacttgtta ttatcaagcc agacggcgta agacgccatt
    24001 tggtcggaag catcatccaa cgttttgaag caaagggact ggccattgct gaaatgaaat
    24061 ttgcaacgat gacacctgaa ctagcaaaag aacattatca gcacttgact gaacgtagtt
    24121 tttttgatga gcttatcgat tacatgactt ctggtccggt cgtttatctt gtgctggtcg
    24181 gcgaggaagt gatcgatatc gtccgtaaga tggtcggagc aacaaaaggg gcggatgccg
    24241 ttccaggtac gatccgagga gattatgcac tgccggggac tgaaaacatc atccatgctt
    24301 ctgattcccg tgatgctgca acaaaagaga tcgcccgctt tttcccgcca ttcgatacaa
    24361 acaaaccgtc tgaaaaagcg gcgaagtgat acaaatccta aactgttggt ctaactgctg
    24421 ggtttagcaa tggccagcag ttttttctct aaagaattcc tttgattctt ccctaatgta
    24481 tattcgatca ctgcgaaaaa ctcttggtga ttttccgatt tttcttcccc taaagcagca
    24541 gaaatcagtc gttggtaata atccgccatc aatgtttcat aaatcactgt acttctttct
    24601 ttatgcgcta aactccgttt catatcttca ttcacgctac gtgccttttt taagcgacca
    24661 ttttctatat aaaacaaagt caagttcgtc aaaaaagtaa agagatactg ctcgtgatta
    24721 ttcagttgcg cgatccgtcg cattcttttt ttgattttca aataattatt ctcaataaaa
    24781 cgatcactga atatccaagg aaaatctgct agaatagata cttcaaattg cccatactct
    24841 ggtacttgca tcaaatattc ttcaatgata cgacattcat aacgcaaaaa ttcaaattcc
    24901 gtctcagata cttctgtttt tttggacatg tgcagattca aaaccaaata gtacagatct
    24961 tctgttttct tatagtgcct gcggttatta gcaatttctt tgtaaggatc aaagctgtct
    25021 tcttgaatcc gatctagcat tcgtcttttt ttctttaatt tttttggctc atcaagctga
    25081 tttcgtatcc attcatattc ggcaagtgtc atgttcattc gctctaaaaa tgatttcaat
    25141 gttccaaacg atactttttt cactgaattt gtctcgatcc cgactagcga agttttagaa
    25201 cagattcctt ccgctaattg ggtctgtgtc agctttttgt tgactcgaag cttcttaatc
    25261 aattctccat catacataat agttctcctt ttttaattga aattaaccct tggcttttcc
    25321 agaatgcttg ccaataagag gaatgattat ttcaatttaa attttccgca ttcaataaat
    25381 agatacgtta aaaataaaaa atacacgaaa aaaataaaaa aatgtttttc tcataaaaaa
    25441 taccaacctt caagcatgag actgctgtct aatccctgaa ggttggtttg attgtttatt
    25501 gatcattttt ttactgctcc gcattggttc ctgttactgg atataggata gtcgttgttt
    25561 tattgccgtt ttttgaaaag acactggtcg aatgttcacc tttttgcttt tcgattgctt
    25621 cattacgttc cgcttcgttt ttatagtcat attttttcgg atcaatcgtc tcaaatccgt
    25681 ttggcacata aaaccgcaag agattttcct gattcagctt atctgaaaga cttaacgcag
    25741 tcttgacttt ttcctgatct ttggagattt cctcgtcgat tcctgctgct ttcgtatcga
    25801 tgatttctcc tgtctgatta ttgtaagctt ttcctcctag gacggtgtat ttgggcgtca
    25861 cgaagttccc atttcgaaaa gcaactactt gatcgtgctg agaagataat aagtctgtac
    25921 caaaatgaag ataatcttta tcatcgattc ccaatagatg tagtaatgtc ggcaaaacat
    25981 cgatttctcc accatattca tgattgactg tccctttagt gtaaccaggc atatggatca
    26041 tcaatggcac tcgttgcatc tgtgcgttgt caaattcatc ccatgtatct ggatctttac
    26101 caagagcact tgctaaatct ttattatctg tattcgagat cccaaaatga tccccgtaaa
    26161 tcacaaagat tgaattttgg taaataccag attttttcaa atacgtaaaa aattgttcca
    26221 atgattgatc cagataatgg gccgttcgaa cataattatc tactgtgcta ttgccagtat
    26281 tcaaagatgg aaagtcgaaa ttcttgtcat ccgtataata aggcgtgtgg ttggttacgg
    26341 aaagaaattt cgtgtaaaac ggctgttgta gatgttcaag atattgggca gattctctga
    26401 ataaatcttt atctaatatc ccataaccta acgtttcatc ggattcatca aaataagatc
    26461 gatcaaagaa attgtcatac cctaaatttt tatatacatg atctctattc cagaaactag
    26521 ctacattccc atggaaaaca gcacttgtat agccttctgt ttgaccaagg gtcgctggtg
    26581 ctgcttgaaa gacattatct gacccaagtt ctgtgaataa tgagccttga ggtaaaccaa
    26641 atgtgccagt ttccaacatg ttttcagcat cacttgtctt tccctgtcct acttcatgaa
    26701 agaaattgtc aaaactgatc gttgcttgat tctggaaaat actatttaaa aacggcgtga
    26761 cttcttgtcc gtctaccttc atattgatca aaaactgttg aaaactttct aaatgaagga
    26821 caatcacatt ttttccttta gcaatcccaa atgtttcagg atttggctct gcataatgtt
    26881 tctttgtata gtctaaaact tcatcaattc cattactgct tgcatgcgcg cgaacactgc
    26941 tagtcattcc tgttttgatt ccgtcataaa tagtgtacgc atcgattcct agatatttga
    27001 caatatagga acggtcaaat gtccgctgta gcaattgggg acggttggct tcgctcaacg
    27061 ctaaatttcc tgaaaaaatc aatcctgaaa gacaggtaac agcgatagcc atcggtttgc
    27121 tgacagggtt gcttttgata ggtattttct tgaccttcaa ccaaaccagt agcccaatga
    27181 acacagcgat atccaaccaa tagatgacat catggggttt catcaaagaa aaggagcttc
    27241 ctgacaaccc ttgtgacact ttcgaaaagc ctaatacaga tttgactgta atgaaatctg
    27301 tgaattcccg ataaaaaata atattcaagt acaacaaaag cgtatttaaa atatcaatga
    27361 tcaaaagaat ggctaacgct ggctttggct ttttaatata caaagaaagg ccaaaaaaca
    27421 ataaggtagt cgcaatcggg ttgatccaca agataaagta ctggatcgta ccttccacac
    27481 ctaatgaaaa atctgcatag taggcaatga tcgtcttcag ccagaaaaag aaaacagctg
    27541 tcaaaacgat tgctaaacga ttttggcaca attttttaag ttgtgtcacg tatatcactt
    27601 ctctctattc acattttttc ttattttgct cctgaaaaat gaaaatttcc taaaaaagtt
    27661 gttatcttaa gattttttta ttctttctaa agattattcc attcattttt cacagcataa
    27721 aatcgtttcg gtgtaagcgg ttaaaatagt aaggagataa aaatcttgtg gtgaccgatt
    27781 tactctatag tattcaaaag tagtttctcg gtattcgtac aaaaaaaaca tactaagatt
    27841 agaggggaac atcattgacg aatgatgttc tcctctattt tctattttta gattatagtg
    27901 aagagtgaaa gaaagttcga acaaagcctt gaggactacg agtccctatc aaaaaccgaa
    27961 cttctttact gtcattttag aatcaggttt acgtatgttg gggccatgag cccgtgtata
    28021 ggaaattgga atcgaaatga agcagtaaag ttctttcaac acttaaaaag gaggaaaaca
    28081 aatgccttac attcttgcat tagatgtttc aatgggacac agctacagtg tcctatacaa
    28141 agacgacacc tgcttatttg aagacgagat cgaacacact caaagaggat ttcatgcatt
    28201 actcgaagag atccagcact tgcctgaacg tccattgatt gtttttgaag caacgggtat
    28261 ttattccaga ccagtcgaaa agttctttca agatcatcac ttttcttact gtttgttaaa
    28321 cccgttagaa gcgaaaaaac agatggaaga aaacacctta cgcagttgga aaacagataa
    28381 atcggatgcc catcggctcg cacagactca tcttaaaaat caacgtcaac caaaagaagt
    28441 acaaagcaat ttttacttag agatgcgcga tttagcccgt ttttatcaag aaattgagaa
    28501 gaaaatcact cgactgcgca tggatttaca caactgcctt cagttgactt ttccagagct
    28561 tgaacagttc ttttcaaaca ggctgacccc ttacgcattg acattgatcc gtctttttcc
    28621 ccatccagat tttgtattgg catctactcg gacaaaaata aaaaataaac tgatcaacga
    28681 aacacgggaa aaaatctccg cgaatcgtgc cgagcaaaag gcagatcaaa tcatccacta
    28741 tgcacagtgt gcctatccag ctgttgaaaa agacagtatt cactgtcaaa aaacgatcta
    28801 ttacgccgaa cttctccaag atttactcga acaaaaaaaa gcactggcga cacaaatgat
    28861 caaaaaagcc gaagcctctc cttgcttttt gttgtaccaa acgttccctg ggattggcgc
    28921 actcacagcc gctttattgc tcggtgaatt aggagatatc acacgattta aaacccataa
    28981 acaattgaat gcctttgtcg gaatcgacat ccgacggtat cattcaggta agtatactgg
    29041 ccaagaccgc atcaataagc gtggtaatcc caaagcacgg aaaattattt tcttcaccat
    29101 tcgaaatatg attcgccagc aacgcgcagc tcctaatcat attgtcgatt attactacaa
    29161 attaaaaaag caacctatcc ctaagaaaga taaggttgcc acagttgctt gtatgaacaa
    29221 gcttctgaaa tgtatgcacg ctatggttag ggcacataca gagtacgact atgcgtacgc
    29281 ggtctcagtg gaccattaac taaaccactt acagtatacc acttctttgc ccagatcgaa
    29341 aattttttac gaacggggct ttatttagtg tgccttgatt ttgtcgcttt cgtttacgag
    29401 ctatttttct tgcacctttt acttgactaa tcgtaggaaa gaggctgtga caaaattctt
    29461 gtcgcagctc cctatttctt gatgcaggac agctttttca caacctctcc actaaacggt
    29521 gttcaatcag ctgaccttcg gtattagctc aaaaaacggc aaaatatgag gagctatttt
    29581 cccgtttttt tctctaatac tcaggtcaaa ccgctgattt cacaacctct gattcacggt
    29641 gttccagaag tagcttcttc ggaaataagc cgaaatttta caaaaacttg agaagcactt
    29701 ttcgtaaatt tcttcttatt tcttgaagct gaccacttct gtcacaactt ctgtttttca
    29761 ttgtccatag gtttgatatt gtccttctga tgactcattc gtaatgattg aggcttgatt
    29821 ttcttcattt cccgttatcg tttgtccact gccagaatct gaagggctta gttctgtcga
    29881 ctcgtttttt tgagaagaag cagttgtttt cttgacttgt tcgttggtct ttttccattc
    29941 cgctagtcga tcggccacca cttttttgtc cactttttca gaataagctg taaccacttt
    30001 tccatttttc aaacgtgcta ccttgtcgcc atcatgagtc aaaagcaaaa cgaccaaatg
    30061 tttgtctttg aattgatatt gtctgagaga aatcgcacta agtacgtcat cgatattgat
    30121 aaattgattt tcttcttttc cttttggcaa atagatgtcc aattcacctt tttcgtaatt
    30181 ggcagttaat ttatattcct cccaccaata agttgagaat tcttttttga aattatctac
    30241 tgattctttg cttgctacga ttttttctgt ttctttctga gcaagatctg ctgtcatttt
    30301 tcctgaaaat gctgcggata aaataagcag taagaaaaaa ccgaataacc aaaagagaag
    30361 aaacggtctt tttttcctca tgccttcctc cactcctatt taattttgac tattctatta
    30421 tatacaaaaa atgaaataga aagacaaaaa agttgaaaaa gatcgtaaat aattgaactt
    30481 ctttttcctg aagcggtaag aacgggaatt ttcatacatt ctctcttggc tattctttaa
    30541 gcttcaaatc catttctgtc tgatacatct ttgaaccaat gacccgattt cttgatcgtt
    30601 ctttttccat cttgtgctaa atctactgaa ataaaaccgt aacgattctt ataggcattg
    30661 gtccacgacc agttatccat gcatgtccac atatgataac ctaaacaatt ggcaccttct
    30721 tgaatggctt gatggacata tttcagatga gattgaacaa attcgatccg ataatcatcg
    30781 tggatcatac catcttcctt gataaatcgt gcttcatttt ctacacccat gccgttttca
    30841 gaaataaaac agtcgatatt cccataatga tctcgtacat tgatcaaaat atcatagatt
    30901 cctttttcat agatctccca tccgcgataa ggattcattt tacgaccagg catttcataa
    30961 ttgtcgaagt aatcctcagg cataggaccg tttttagtat cgattggtgt ttcttttgct
    31021 tttattcggc gtggttggta ataattaatt cccaaaagat ctactgtgtt ttctttaatg
    31081 atcgtcaaat catctggatg aatctccggc attagatcga gttcttttat tctctcaatc
    31141 aattcactgg gaaactcgcc ttttacagct ggatcaagga atgaacgatt gaagaacgca
    31201 tctgctattc gtgctgcttt aacatcttcg ggatttgatt cgtctcttgg ataactcggt
    31261 gtcaaattta agatgattcc gatcacaccg ctttgatcca tttcatggta agctttgatg
    31321 gctaatgctg aagccaacgc ttcatgataa ccaacttgga ttgccttttt catatctgat
    31381 tcttccggat aatgccatcg atacaaatag cctgcctcga ctggaacgat tggttcatta
    31441 tgggtaaacc atttttttac tttcgagcca aacaattgaa aacaactttt tgcgtatttt
    31501 tcataggctt caaccgtttc tcggttcaac catccgcctt tttcttgcag atacatcggc
    31561 atatcgaaat gatacagatt aacaaagggt tcgataccgt tttcaatcaa ctcatcaaaa
    31621 tagtgatgat agaaacgaac ggcttcttcg ttgattgttc cagattcatc aggtatcaaa
    31681 cggctccatt ggatagaggt tcgaaatgaa ttatgcccag tctgtttcat caactgtaca
    31741 tcctcttgat acttcgtata aacttgcgag gttttttcag gtcctacttg atcaaagaac
    31801 ttttgcggtt cgtttttgta ccaataatca aaaatattct ctccttttcc atcgcctgaa
    31861 attcttcctt ctgtttgtgg accacttgct gcagagcccc accaaaaatt ttttggaaat
    31921 tgatatcttt tcatcaaagc acctcttttt cttttttagt tgattccatg tatgatattt
    31981 tccatcacaa tcactttttc attttcttcc tcttcttttt tgattcgttt caacagctgt
    32041 tctgctgctc gttccccgat tttatctgtt ggtggcgtga tcgtgatttt tggaaggata
    32101 ctcagatcgt ttttatctaa ataatcatag tgactgaacg caatatcctt cccgattctg
    32161 acaccttctt gattgaaaca ttcgatggca ccaatcgaca tgttgtaatt gctgacaaaa
    32221 atagcagttg cccctttttc taataatgct tttgccccgt aatagccgct ttttctagaa
    32281 tagtctcctg taaagatctc taaatggata gacggatcct tttgtaccgc ttctttggct
    32341 ccagctaaac gttctcttgc tacgtaatct gtttgagcgg cagcaataaa tcctattgtt
    32401 ttatgtcctt gtccgccatg atgagcaatc acttttgcta cactttctcg atcattcact
    32461 actattgtat caatacctgc tggcccaccc ggacaattca atgcgacaac tgggatactc
    32521 atttttgtca gcatttcaaa tccagaccac tcttcttctg ataagaagac gatcaatcca
    32581 tcgatcgcat gcttcatcag gtattcgatt ttttgttcga ttttttctgg ctgatcggca
    32641 aacccgctta ataataagct atacccattt tcttcagcta ctctttcgat ccctgaaaca
    32701 acctccgaac caaatcggtt agatatcccg tttatcaaca gtccaataga gaaacttcga
    32761 ttattcttca atcctttagc aatgatgttt tctttatagt ccaacgctga aatcgcttct
    32821 gatatttttt ccatattctt ttccttcagc tgctgaccgt ttaaatagcg ggaaactgtt
    32881 cccactgaaa caccagccat cttagccacg tcacggatcg atgccataca ttttcactcc
    32941 tgccagttac atttgatttc tttttcttgt tgttcgacca tgatgaccgc tttctttgca
    33001 taatgccgct tgtcatcaat atccatataa ctaaagtctt cagaagtgag ttcaaaaaca
    33061 agcagtcttt cctcttgaga ccgcaattca tctttacgaa aagctttcaa taatttcgtt
    33121 ctttggatga caacgccgtc aattaatcgg acatagacaa gagtacttgt tttacctgaa
    33181 acacttcctg tatttttaac tttaattgat attattactt tgtcaatatc cgttttgact
    33241 tccatctgac tgtattcaaa tgttgtatag cttagaccat gtccaaaagg ataaaatgga
    33301 cttccgctta aatcataata attctcttgt ttagaagcag cacgctgata gtaataaaca
    33361 ggtagttgcc gactatttct aggataagac aaactcaatc gtccgcttgg gttatttctt
    33421 ccagaaatca tctgcgcaat ggcttttcca ccctcctgtc caggaaacca tgctgttgca
    33481 atggcattcg taaatgactc gattttcatc aaatcgtagg gtcttccttg cacaagtatc
    33541 gtgatgattg gcttgcctag ttgatacaat tgttcaatca gctgttcttg gtatccacct
    33601 aaagaaagag aagcaacatc tacattttct ccagaatcca tgttgatccc tttcgatgaa
    33661 actgccccgt tacgaagaaa ttccatatca aagttccgcg cacttgatcc acctagaact
    33721 gcaatgatca catctgagtt ttgagcaagg gtcaccgctt ctaataattt ttcttcctga
    33781 tcctcctgat ttcttacttc acatccttgt gcataagaaa cctttgattg gggaaactct
    33841 gtttttatct cttgtactat cttttttttc aaacggtctt ctgtttgtgg tgcagagtag
    33901 tctcccaaca agtggtaaac atcgtgagca tttggaccta taacagcaat gttttttact
    33961 tgttgatcca acggcaatat accgtcattt tttagtaaag tgatactttc ttttgctgtc
    34021 tctaaattta agtgctctga ttcctccatg agatgatccc aatactcctt tggatcgttt
    34081 gcaaatggct gttcaaataa acctaataaa aatttgacag ataaaactcg gcgaactgct
    34141 tgatctagta gcttttcatc tacgacttga ttatcaatgg cttctgcaat cttggtatag
    34201 gtctcatccc acaaactgag atcgattcct gccgctaatg catatgctgc agcagtcttt
    34261 ttatctgtaa aaacatcgga taaacgatcc aaagccacac cgtctgccat aacgattcct
    34321 tgataaccga tctcttttct taatgtttgc tcaaaaagcg ctcgattgat atgacaaggt
    34381 atcccgtcga tgtcattata agcagccatg acacctactg catttcttgt actcttcaat
    34441 aatggtccgt aaatatcgga gaactctctg cgtccaatcg ttactgtgcc tgaattatga
    34501 ccgcctaaag cttctccttg tgcgatacag tgctttaaca caacaccgat ctgatccttc
    34561 ttcttattga tttcctctac tgtctgatca agaaaagaaa ctttttctga aataatctct
    34621 ccttggaacc ctcgaacgac cgcttcagaa aaacgagctg agagaatcgg atcttcccca
    34681 aagcactctt ctgtccttcc ccaacgagga tcttttgcca gatccaaagt cgacaccagc
    34741 gctaaatgaa cacctttagc agccagttct tttgccatca atcgactcga ttgttctaat
    34801 aatgctacat tgaaagtatt tccacggccg atattcgttg gataagaaat cccatccaag
    34861 ccttgatgac catgtggaca ttcttctaca agcaaaactg gaattttcca tcgcgaatgc
    34921 tgtagtacat attcttggac taggttagcc accttccagc tttcttttgc aggaatccca
    34981 ttcttataat ctgttttaga ccacggatca gagcgaaaca gaccgtataa ggcacccaga
    35041 ccgcctcccc atttaacatg ttcttttaac ttttctgtta attcgatctt gtctccgttt
    35101 ttttcataac attcccaacc atacaaatgc tggtttacct gtcctacttt ttcagcaata
    35161 ttcatcctcg aaagtaaatc ttctactctt tggcggatgg ttaactttgg gttttgatac
    35221 gggtaatcgt acaccttaat atcctccttc tgccaatttt gccttattag cggctaatac
    35281 aaatggcagg aataaaaata catctagaat gataagtatt actgttaaga ctgctgctcg
    35341 tatatcgcct cctgtagcaa taaaagattg tataaccggt ggagtgatcc aaggtgcagc
    35401 agcaactgtt ttactgatga ttccaagact tgtggctgcg taggcaataa ccaagttaat
    35461 agatggtata atagcgcatg gaatcatata tattggattc atgactactg gtaatccaaa
    35521 cataattggt tccgtaatat taaataaaca agtagtcaga ccaaatttag cgattgatcg
    35581 ctgttcttga cgtttagaag cccacaagat agcaataact aacggtaaaa tacacccgcc
    35641 acctccgatc aatccaaatg cattggtaaa gggctccgta ataatattag gaatttcttt
    35701 tcctactgaa aaagcatcca tgttttcctg aatagactgt aacaaaggtg cttgccgtat
    35761 aggagccaaa atactcgaac catgcatacc aaacacccat aagaaatcag agaaaaactg
    35821 taaaaataag atgcctggga cggataaaac aaaagctttc aatggtgtct gaatcgctaa
    35881 ttcaattaat tgcggaacat tcaaaccagt cgtatggttt aaagtaaaaa cgacaactga
    35941 aaaaaataaa atgacaacta tttctggtac taaagaatta aatgacgcag ctactgccgg
    36001 tggaactcct tctggcatcc tgattttaaa actcttcatt ttcgctaatt tgataaataa
    36061 ttctgtactg acaagggcag caatcatcgc cgttgctaag ccagacgaag aagtcagact
    36121 ttgtaagaaa acccctgtag cttctgctgt atttccgtcc atcaaagtga ctgtacttga
    36181 taaagacatc aaaataaaca ttacggctgt acttagaatt cctgcatgaa caggactaaa
    36241 tgatggatca tcaatcacgc ctgtttggat atagtgggtt gccaaattcc agccgatatt
    36301 gtaacacacg ataatagcta aaatactcat cgtaccatta ttcacgattg aagcaagttc
    36361 agccaatgga cttagatcaa gtattctttg gactgttgca tttgaaaaaa tcagagcatt
    36421 cagcaaaacc atgacagaag cagccattac taacggcata gtaacgataa aactgtctcg
    36481 aatggccagc aagtgtcgct ggctactcat cttagatacg attggtagca ttttttcaga
    36541 tagtctgtct acgactttca ttatttcatc tcctagattc taaaattgaa ccggttcatt
    36601 tttaatatag cacttttttt aaagattgca acacattttt ttaaaacgct ttctaaaggt
    36661 ggtggatttt ttatgacttt tgaaaaatta ttctatttaa tggtattttg tgaatttttt
    36721 ctcttccatc cgtttgaagt tttactttcc aatggtataa tttctttatc ctatcaaatg
    36781 ggggaagtat tttgaagaaa ttaatcgtac tatttatttt gtctgcggga cttttgacat
    36841 tgactggctg tgatttgttt ggcggctctg accaatcctc ggacgaaaca accgattctt
    36901 ctgtcttaaa agcggaaaaa gcagatatca ccaacagtgt atatacgcta aaagtacgta
    36961 aagatatgga tttatccgcg ggacagttga catttacaga taaaaaaatg gaatggacac
    37021 gcacgtatca gacacaagga gctgattctt ctgaatcatc tgaagaaaaa acagtgctga
    37081 cagatatcaa gattactacc aaagatgacg aatatattat ttcaggaaaa gaaaataaaa
    37141 aagaagcaac agtcacattt accaaaatcg gtaattaccg tttgaaggat aaggatggca
    37201 acatctacag tctatagtgc catctgttgg atcttctttt gtttctcccc ttcttttctg
    37261 ctatagtaaa gaagcaagat gactgtaaag gagacgaaat ggatgatcga tagtcctgat
    37321 tattgtacat gtaataacaa caaaaatatc catacagaat ttaatgatca aggaagtttg
    37381 agtatatgcg acaattgcca caaactagtc aaagattctt ttaaatcgtt tgaatcttat
    37441 cctgtcaaaa attatgtaaa aagcaaaaga agataagcaa aatcaagcaa aactcactat
    37501 ggctgatttt tatgaacttc aaactcagct tcttcctttg atcaagcttc aaaaactgga
    37561 atcagtaaaa caaacagtgg atcatttcag ttcacaaatc aaagagtaac gttttattta
    37621 tttcacatct ttgaatataa aataaccaaa cgatagaaag acttattcta catcacgtag
    37681 taatttttct ttcccgtcgt ttggtttttt acttttagcc taattcttga cctttcgcat
    37741 gctgattatc tgtaaaatat tttccagcag gctttccttc caattcagct aatttttctt
    37801 ggatctgacg aatcaccttt tttgcatctt ctggattttc tacaaaatcg aatgtatccc
    37861 catccacttg tattttagga catacatcaa aatcctcata ccattgatta tagcgggtgt
    37921 tcagtgtttc gtaataggtg tataacgttt catcgctttc tagctgttca tagtcacgcc
    37981 cgcgtttttt gatacgttct agcatcgtgt ccaaagaaac tttgatatgg accatcaaat
    38041 caggacgttt ttttggagca acatcatcta actcgtttag catcgtatct aataagctat
    38101 cgtactgctg tacttcgata tccgttaccc gacctaaatc agcatttaga tggaacaaca
    38161 gactatcttc gtagatggag cggtctagca cattgtcatc gttcactaaa gcatttttca
    38221 ttgctaaaaa tcgcttattc aaaaagaaga tttgcaataa aaatgcgtac tgctcaggat
    38281 ttgaataaaa aagcggcaat acttcgttat cttcgacatt ttcataaaat ggtttagaat
    38341 caatctcttt tgcaagcata tccgtcaaac tgctcttacc tgcaccaatc gttcctgaaa
    38401 tcaaaatcac tgacatacaa cccttcctct tctataagta ctcgcctacg taagtttcaa
    38461 caggcaattt ttcaaaatca tattcacgta cagcttggtc tgattttaaa tacgtgtgaa
    38521 gactttgtgt aatcatcaag ttcgtaggta ccgtctcttc ttgtacgatg atcatcggtt
    38581 ttttcaacat agttgccaca cctaattcgt aagccgttcc tggatcgatt gtttgatgct
    38641 caaagtcaag gatagccaaa acaaattctg catttgtcaa ctcttccata tctttttcgt
    38701 atacttcctg cgcccatcca gcgctgaata gttcataatt gctttcttgg tgatgacgtg
    38761 gagagtagaa acttgtgact gttttgtttt cctccaacgc tttttcgata cgacttactc
    38821 gatcgatttg ctcctctgaa aaaaatggtc ctgctaaata tatttgactc attattttgc
    38881 ctccttcaat aataactgat ttttaataga tactgctcgt aattcattga ccagttccaa
    38941 tggattgttg gaaaagatca cttgatccaa ccatttttta tccataaaat cctcttttgc
    39001 aaaagtttct aataattgtt gcaagccgct gtaataaccg ccaatattca atattccgat
    39061 tggcttatca ggtgtcaggc cgatcgctat ccaagaaaga agttgcgaaa attcctccaa
    39121 tgtacctaga cctccaggga aaacgaggac agcatctcct ttttcaaata ataatacctt
    39181 tctttcatca attgtctctg taggaataaa tgtagtaaca tctttcccag gcagctcagt
    39241 ttcaaaaagg ccatttggat agatacctgt gacttttccg ttattttcca gtactgtttt
    39301 actaatcact cccataatcc cggagtatga accaccgtaa accagttcga tttcttcttg
    39361 tgccatatat tttcctaacg tttgcgcaat aaatttgtaa ctttctttat tgccaaaacg
    39421 ggaaccgcaa aaaacagtaa gtttcataaa aaaacactcc ctaactcaaa agtctatctt
    39481 cattctacac gtgttttttt aaaataaaag actagatttt gtagtttttt agtggtatat
    39541 agatcaaaca ccactatata tagtatttgc aaaaaaataa tttttcagct attttacagt
    39601 aaccaaaaaa ataaataact agtaaatata aaattgaaac gacttactta tagaagaaat
    39661 tgggatttcg tcctgcatgt caataacacg cattatttaa atagtcacac acttgtttct
    39721 cctattaatg aaaaatccat tttattctcg tgttatgttt caccaacgcc tgatgtttct
    39781 ctaaataaaa aagccgacga accaaataat cgaaatttga tcattttggt tcatcagctt
    39841 gttcccaaac ctttgtatta acgataaata gctggattcg taaaactatc acttggatca
    39901 tcactgctgg aagaaaattc ggtgataacc gcgcctgttg cacctgcctg gaaccagtgt
    39961 ttaatatttt gttcgatcgt gtactgatct ccagcgtgaa gtatgatttc tttttttgcc
    40021 gtatagtaac gttcttgccc ctttggaata atacaggaaa gtttttttgt ttcttttcct
    40081 tcgacataaa gatatactaa gccttttcga caacgaaagg tttccttttt tccttcattc
    40141 tcatctcgag gtgggtgccg atgctctgga caagtttgat aaggcaataa taccagttct
    40201 ttcccacagt atcgctcatt attttcataa acaaataaat tcaatccttc agtctcgatt
    40261 gctcctaatc caaaatctgt atattcgatt ctttctaatt ctgtttcttt caatactact
    40321 cctgaatcta ggaacatttg ttgtactctt ttcttgtaat ccattctgtt cctcctaact
    40381 gattgtcaag tcatcccaag atatctcttc ttcctctttt tcttcttgga tgacttctgc
    40441 atttgggtca actggttttt tcaataataa aatagttatt cccgtagcaa aagaaccagc
    40501 taacaatgca ccaatgaatg cccatggtct ggtcatcgta gcgactgcaa acattcccga
    40561 tgccggaata cggctatctg cgccccaaaa catgctcaat ccaccgccga ctgccccacc
    40621 gactcccgtt gctatgacat tgcgtgccaa gtcgttcaat acgataggga aacatccctc
    40681 tgtgatctga cagatcccca taggaaaagc ggtcttcaat gtatcgattt cttgacgatt
    40741 gaatatattt ttacgaatga ttttccctaa gaaataagcg atcgtcaagc cgaatggggt
    40801 taccatcgaa gctaaaatca atacagtgat tggttcatag attccttctg aaaatcgtgc
    40861 cacaccccct tatccagaat aaatggtaat ccagaaacag ccgggtatct gcggtaataa
    40921 gcctaaccaa acgaaaaata tgagaagcta atatgagaag ctatttttgt ttggctgttc
    40981 ttattacttg atgcagggca gctttttcac aacctctttt tttatttatg tttcataatc
    41041 aatatggtaa ggatgcagct ccgtatggta gaccgcctcc gaatattcaa tcatttcccc
    41101 aatttgcgta gacgatttcc gtatccgttt gatgccgatc tgtgcgtctg ttttcagaat
    41161 ttcttgttct tcttctgtta aaaatactgc cgagaaatca tcagaaaatg agtcaatagt
    41221 atatcctttt ttcgtcagca aacgatacaa agattctttg ccaaattctt ctctgctgaa
    41281 ttgcatcatt tcttgtggaa gataatgaac aaaataaata taaggctgat tgttcaaata
    41341 atataagcgc ttaaaacaaa cagcttgctc accgaaaaag gaaaacaaag gatcttcctt
    41401 ctttatatgg accaattgca aatccagcgt ttctttccgg acttcatatc gggagttctc
    41461 caagatttgc gtaaagcttg ccgctttgga taatcgattg taaggacgat tgcttagaac
    41521 cgtcgtccca cgaccacttt tctttgcgac atattcatct gatgccagca gttcgattgc
    41581 ctttcggaca gtgattttgc tgacttgaaa cagttcttct aattcacttt ctgtcggtaa
    41641 catcgaaccg actggatatt ttcctgagaa aatatcttct ttaattttat ctgcgacttc
    41701 caagtatagt acacgtgtcc ttttcataac gtcacccttt gctttactat tactaaaaga
    41761 atagcacata aaaaataaaa aaagaacaca aatcacatgt gagaagtgtg ttctttttca
    41821 ttcagctgct ttaattacaa gtttacgcca tttgaggcaa ttacttcttt ataccaatca
    41881 aaagattttt tctttgatcg attcagtgtc ccatttcctt catcgtctag atccacatag
    41941 ataaagccgt aacgtttact catttctcct gttgatgcac tgacaagatc catacatccc
    42001 caaggcgtat agcccatcaa atctacaccg tctgctactg cattagccat cgcttcgata
    42061 tgacgacgta aataatcgat ccgataatca tcaataatcg tgccatcctc ttccatatga
    42121 tcaatggctc caagaccgtt ttcaacaatg aataatggtt tctggtaacg attgtataat
    42181 tcattcaatg cgattctcag cccttctgga tcgatctgcc agccccattc acttgcctca
    42241 agaaacggat tttttactcc tcccagcaag ttcccttctg aagttgctgc ttgtggatca
    42301 gtctcattga ttgccgtaga catataataa ctaaatccga tgtagtccac tggatattct
    42361 tttagcaact ggaagtcttt ttccgtttgc tctaattttt ccggatcgac tccatatttt
    42421 ttatgggtac gcgctgtgta tgcagggtat tctccacgta cttggacatc cgtgcagaaa
    42481 aaattgaatt cttggttttg gatcattgtt gctgcctgat tgactggatt ggcatcgatg
    42541 ctgtaagttg ttgcatagat aatcatgcag ccgatttgga tatccgaacg caattcgtgg
    42601 ccgattttta cggctttgct acttgcaacg aattgattat gccatgcttg gaaaatattt
    42661 tggtactctc ctgctccatt gctttttact aatccttgac ttaaagctgg aaagtgaaag
    42721 gccgaattga tttcattgaa agtcatccaa tatttgactt tactgctgta tctctctaat
    42781 actgtctgag caaatcgttc atagaagtcg atcagttttc gatttttcca gccaccgtat
    42841 tcttttgcca aatgaagagg catttcgtaa tgagaaatcg tgatcacagg ttcaatatca
    42901 tattttaagc actcatcaat cagctgatca taaaattcta atcccgcttc gttaggagta
    42961 ccttcgtctc catttggaaa aattcgtgtc caggcaatcg aaaaacgata acatttgaat
    43021 cccatttccg caaacaaggc aagatcttct ttgaaacgat cgtaatgatc aattcctcga
    43081 tgattcgggt aaatatattt ttcttgatca atcgtccagt caaattcttc actgccgata
    43141 atcgcaaagc gctgcttacc tccaggcata gcatctgcaa cggatagccc ttttccgtct
    43201 cgatcataag ccccttctaa ttgattggca gcagttgctc caccccataa aaatttttct
    43261 gaaaaacgtg attgcatcct gatttcctcc ttattttaca attgccagaa catcttcgtt
    43321 tgcaataatc tctttttggt ctaattcaag tatatcggca aatgcatcag tattcgtaat
    43381 gacaactgga gtgattacgc tatagccagc tgctctaatt gcttcgatgt caaaacgaat
    43441 caataaatct cctgctttga cgagatcccc ttgtttgact aagatctcaa agccattccc
    43501 atttaattct actgtatcca tcccgatatg aatcaaaatc tccgctccgt cagtcgttgt
    43561 gatccctaca gcatgtccag ttggaaaaat agtcgtgatc tctccgtctg caggtgcaac
    43621 tagttctcct acttctggat caatagcgat accttttccc atcacgccag aagaaaaaac
    43681 ttggtctggt acttcactca atggcagtac tttacccgtt aaaggacttt ttaaggtatg
    43741 ccgtgctgaa gtaatcggtt ctcctgcatt cgcatgtttg ttttctaatt gattgtcttg
    43801 tgtttgttcg tcaaatccta aaatcaatgt acctacgaat gcaagaacaa aagcaattcc
    43861 tgtagctatt actgctactg tcacatttga ctctacacca tccaccgtat taatgaaagt
    43921 cggaatcgtc aataagctga ccaaactgct ggagaaagct tttactccgg aaaatccgat
    43981 aatagctcca ccaatacctc cagaaataca agcggcaatg aatggtttct tcaaaggtaa
    44041 tgtcacacca tacacagttg gttcagtgat tccaaataac gaagtcactg tagaagaaat
    44101 ccctaatgct cgaagtttcg tatcttttgt tcgcaatgct acagctaaag cagccccacc
    44161 ttgtgccaag atggctggaa gaagcattgg catcaataca tcaaacccat attgttcgat
    44221 attcaaaaac atgatcggta cgaatcccca atgcatgccg aacatcacga atacttgcca
    44281 taaacttccc atgattagac ctgctacgat tggactaaat ccataaatcg agttgaagag
    44341 accgcctaaa gcattaccgg caaccgtccc gattggtcct aaagcaagaa atgttaatgg
    44401 aaccatcact actaaaacga caagcggaac taaaatcatt tgcagaaatt gcggaatcac
    44461 tttttttacg aatggctcta atttactttg aacccatacg gctagaatga ttggtatgac
    44521 agaagaagta taaccagatg gtgaaagaat cactgggatc ccaaagaaac tgatagcttc
    44581 tcccgcacca gcaactgctg tgatagctgg atacacaagt gccatagcaa ttgctactgc
    44641 taaaaactgg ttggctttga actttttagc tgctgtaaaa gcaagcatga caggtaagaa
    44701 ggtaaagaca ccatctgctg cagcaaacaa tatttgataa gtaccgctat cagctgtcag
    44761 ccagcccatc accgttgcca atgataagaa tcctttcaat accccggcgg ctgccatcgc
    44821 ccctagaaaa ggtgtaaata tactagagat aatatcgatc agacgattca aaatattttt
    44881 ggactcttct tttccatcct ccgaaactgc aagaccttcc tcatccacga tcgattgata
    44941 aacatctgct acattactgc caatcactac ttgatactga ccgccacttt gtacgacttg
    45001 gatcacgtcc gggtcttctt ttaacttttg cgtatctgct aaagattcat cttttaatct
    45061 gaatcgcaac cgcgtagcac aatgtactaa acttttgaca ttcttttgtc ctcctaccgc
    45121 ttcccataca cgacgaccaa ctgcttgatt atccattgtt ttttccttct ttctttttta
    45181 tttaaaaaat aaaaacccag attgaaaaaa acagtcacaa acattcgtga acctattttt
    45241 tcaatccagg ttttgcctgc catacagtaa caatccttat gattggatgc tttttttctc
    45301 acttactact ctttgaatat gaatcgttaa ataaatctgc tctgcgatcc ccatttcctg
    45361 tttttgcgtt tgtcgaagat actcattgat ccgttcgaca caatgaaacg cttttggata
    45421 ctttccttga actaatgcgt agagaaattc atcttgctcg tcagatcctt cccctttgag
    45481 ataacgctgg gcaaaaaact gcagatgagt aatgatacgc tgataattca aagattcttc
    45541 atcgaacacg atgccgaaga atttactgat gatactcagt atatctctta ccattgtggt
    45601 agcggtcatc gtcacttgca tgtcttcgtt tccttgttgg ctattcacaa aatgaaaggc
    45661 aataaaaccg gcttcatcct cagaaagttc cacacccgtc ttttgtttca cgaacaaaag
    45721 agcctctttc gctgcttgat attctttcgg ataaaatttt tttgtttcaa ataataatgg
    45781 attaggcagt tggatatttt cttttgctcg tgtgatcgca taatgaagat gatcggtcaa
    45841 tgtcagataa atattaggcg aaaattcaac ttttagttta tcttcagcca gttcgactac
    45901 ttgtttgacc cattcgatct cctctgccgg aatatcagcc atcagctgct ggaattggtt
    45961 tttagcaaaa gaatctctta aaacaaattc tttttggacc gcttctggcg aaatctcgtc
    46021 cccaatcttt ttttgaaaag ctagtccttt tcccatacaa acgacttctt ccccgaatcc
    46081 atttttcgaa atgacgacat tgttattcaa aattttatca attttcattt tgcttcgccc
    46141 tctcaagaaa aataaaaaaa cctagaattc gcctactgtt tcttagctga attctggttt
    46201 tgcctgccgt acagtaacaa tccatttctt attcaaaatc atcataccat cgaattttta
    46261 tttatgcaag ccttttcata taaaaaataa aaaaactgga acacaagtct atctagaaaa
    46321 caaccaaatc agagatgcac ctctttgcgt ttctcttttt ttcagcaaat tggtttttcc
    46381 ttatcgattc atatcctttg gctgttttta atctgtaacg acttatgtcc cagtatccta
    46441 aaatgttttt gtctctagat tgaaagcaga tctcatatca tctgtcgcat cttttatcga
    46501 tgtaacactt tccagttctc cttgtacaac tagtcgcgtg acggcgtcca tgtcgccaca
    46561 agtaattagt gtgacaattt cttttccctc gacttcattc agtaattggg tatctgtagg
    46621 agcaactttt tctttaaaaa atgttttgta tgtatacaca ttttctaaat ctgtcaaata
    46681 gatcttggca cccatctcta aattttctag cggtgtaaac agcaattcag ggttcgttgc
    46741 acgatggctt gctaaggcat aatttccttt tcccatttct tgatctggag ataatgttcc
    46801 tgctccgtaa agtagtgacg tattatccaa gcctttgaaa accggtaaat tgattgaaac
    46861 tgaagggact gcgatactag caatcactgg tagatctgtt cctctcattt gcgacctcat
    46921 gaccccttct gtcgtcattg gttctaccgc atcaaagtca aaactgacat ctttatcgtt
    46981 attctttttc agatcttctt ttgttacatt cgctatagca tatttatctc ctgtctctct
    47041 gacaaaataa tccttgatct gttcattgaa aatcaaagct agtcctacta ataacaagag
    47101 gaacaagagg atattgacta gccatctttt tgcttttcct ttttttttca ttttctctcc
    47161 ctccagactc attttactgt ttttcttttg ctctttccaa cagccgaatg acacatttct
    47221 acaactctgt gtattttttt cgtacacctg cgcttttttc tacaggatat ttcaaattat
    47281 ttttgatcaa tttttcttca atgatctcat ctagatccag gtgaaggttg tctgccatca
    47341 tgtatgaata gatcaataca tctgccagtt cttctttgat tcgttctaat tgtgtttctt
    47401 ttacttcttc cggcgttttc cattgaaaaa gttctaacaa ttcagatgct tccaaagaaa
    47461 tagagatagc caaatctttt tcgttatgaa attgtcgcca attacgttca tctctaaact
    47521 ggttgacttt ttccatacta ttcatttgct tgccttcttc caatcacttc ttacttgatt
    47581 aatcatacca tttttttaca taaaaaatag atgatgcttt taaaaaaact cctgttttct
    47641 tttatttaaa ataaatgtca tagaaaatca ataatttagg tagccaccat gtgtatgcag
    47701 tagcgtgtct tcttcccttg aggctttttg tttttcgata cgttcttctt cccaaacaga
    47761 caacgctact agaatctctc ttaatgattt tccttcttcg gtgagcgagt actctacttt
    47821 aggcgggact tggttatagg cttttcgatg gatactttca tcttgttcta actctcttaa
    47881 ctgctgggtc agcatttttt ttgtgatcgt agggatttgc ttgcttctcc acgcctaaaa
    47941 taagcgatat tgctcttccc tttatctggt gtaagcaaag cagttagcat tttgacgatc
    48001 gtgggttccg cttttcactt cttatctact tagtatcatg aagtatactt tcgtactaat
    48061 actaagtagc ggaagtgtca actactttgt ttaactacat agcaaaagaa acagaagggt
    48121 gacaacggtc atcacgcttc tgtttctttt gaatatgctg tgttgttgtc tctatctccg
    48181 tgtagacttt cctcaaactt atcctttgac agtaagtcag taggctgatt tgctctttct
    48241 agatgccaga tacatttcta caaaaaaata gtgattctgt tttgaaaatt cgatttttag
    48301 ttctgcattt tcgtaaacat ccaacatttt tttcgcactg tatagaccta acccgccatt
    48361 ttttttagag gtttttcctc ttttgaccat ctccgatagc gtcaaattcg ttggatttgc
    48421 cttgttcgct atactaaaac gtgatccctc tttttcttcc agcagattga tcgtaatcgg
    48481 actctgatcg gctacacgat gatcgaatgc gttagaaata aaaatagata aacagcgaat
    48541 aaaaacaacc agatcaatcg ggattgtttt cacttcgaca ggaataacta cttggatcgg
    48601 gattttctcc gattctgctt tttctagaaa ttttaaaaag acacccctga cagctgggga
    48661 tacaatattt gttagctgtt gtatttgcgg tttttcaaaa atagctgcag aatgatcttc
    48721 taatatttct aaaaattcca acgtttcttt tttattttcc tgaagagtaa tttttaatga
    48781 aaacagtaga cttttataat catgtttgaa ttcctctaga tgatggtaat attcttcatt
    48841 tatttgatac agttgaagcg attcctgatg ttgtctttct tttacttgga aactgatcaa
    48901 cataatggaa aaaaacaaag aaaaaaaact gatgactatc gacaaaagag atagaagcag
    48961 ataattagat gagcggaagt gaacacttcc caataccaag taaatcacgc cccagcttaa
    49021 taaaatacaa atacctccaa aatagtgttc agttggataa gtaaaaagca agttgagaaa
    49081 tctcgtctgt ttgtctatac tttttactaa tagatagaaa ataaatgcga taagtagtgc
    49141 aaaaaaactt gctgttccaa gatgtctctt tcctaaataa tccatcagat caaaaaccag
    49201 aaaaaagctg agtccagtga tagcaaaaaa aagattactg atcaaacagc tgataaacag
    49261 gtttttcgta gtaaaataaa aaagaaacat caataataag ccaattaggt aaggaaagct
    49321 cactttaggg ataggaagta aaagtgtcac caccatcaaa gctatacaac caaagctgtt
    49381 ttttactagc agttgtttcg aaaagaataa atagatacct aaatctaaaa tcaaagtaaa
    49441 ggtcaaataa agtgtcactg ttgcgaccat cttattcctc cattgttttg tttcaaaatg
    49501 tacacatatt tttttaatag gagcctgcat atgaaaaagt attttatcat agaagataac
    49561 cagtttcaca gcgaatggat catcagagaa ttggtcaatc atcatatcga gaatatcgtg
    49621 cagctcactt ctatctctca aatcaatcag ttgatccagt tgggcactat tgataatcac
    49681 gatgttttct ttatcgatat cgacttgaag caatcactca caggtattga tgtcgccgaa
    49741 caaatacgta aaaaaaatgc acatagtaat attgtttttt tcaccgctta tacgaatttt
    49801 gcagtagaga tcatcaatcg gaaaatcctt ccgattggat atatcagaaa agacaggaat
    49861 ttatctgaac aaattcgtca agtgctagaa cagatcaatt ggaaagaaca gcagatcgta
    49921 aatgtcgata aaatctttat tgatcaaaaa ggtggaagtc tcgtcctgta tcctcgggaa
    49981 attttatata ttgaaagtat caaaggtttg aaaaaccaag ttatggtcaa aacaactaca
    50041 gaagataaat tagtgaatac ttctttaaaa caattgaaga cagagttaga aaagtatgct
    50101 tactttttac ttttaaaatc ttactgtatc aacttatcgc aattgcagaa aattgattct
    50161 gctcattatg tgttagaatt tttcaacggt gactccttac tcgttggtcg aaagatcttt
    50221 gaaaagacaa aggaacgttt ccatgacttc caaaaaactg cttcgagttg atgaataaga
    50281 gctgagacgt aagcatagca gatttcaggg agtgggcctt gaatcggctc cactccctga
    50341 agtcgttgat atgattttac gcttcctctt ctgtaggagc atctgttaac gaccatgtga
    50401 atgttgaaac gtagttgtct acagcataat tggatacagg aacaaataat tccactgatt
    50461 gtctcccttg ttccacattc tctcctaatg aataaatcca tgtgccaatc ccttcatttg
    50521 ctcctgcagt tactagatct actgcctgat ctgctgggat ttcgattcct tcagcgccag
    50581 ttccagaaac ggtagatggc attccactgc tattagttgc gctatttcga atcacggcgt
    50641 ttttgaattt cacctttgaa ccagttagtt tattcgtatc agatgttgta ccaataaatt
    50701 ctgttcgttt gactgataat ttccaaccgg cataatttcc gcttttatcc gttacttgta
    50761 cgaaattctg tacttgtgtt tgtgctccag tactgtccgt tccgctttgt aatgcagcat
    50821 atactgtttt tgctgtacct aacttgattg ttccaaaatc aagatctgga gcttggtcaa
    50881 tcgtcagtaa tccggatgtt ccctgatttg gttcgtcagg tgtaaaagga gtatcaggat
    50941 catttggatc aacaggagga actggaacaa cgtcttcagg atttgcagca tctggtttgg
    51001 tcacagaaat cgttccttct gtagtggctg agctcttatt ctcagcaaaa acactttgtg
    51061 tacataataa tagacttcca gctagaacgg tacttaatgc aattgttttt ttcatttctt
    51121 tttccttctt tcttttaaaa taaataatcc agcaaataag ccaataagaa tgactccaac
    51181 aagtatcagc catcgccagt caaatgtttc tggctgattt tttaaatcaa tatccttttc
    51241 attcaattgg tccgctgcat catcagttat ttcaaaatcg gtatggagat tccagcgttt
    51301 ttctttccaa gcacccttca caaccgcttg ataatgtccc ggagctaatt tttttccttc
    51361 taatgggaca agtaaatcaa aatttgtatt gggtgctaca tctatttttt ctttttttat
    51421 tgaaaggtaa ggctcttttg tttctttttt gtagatttca accgatgtag tcaagccgaa
    51481 catataagaa gcatttggat tttgaagatt cgccttaatc acatttcttc catcgctttg
    51541 atccgcataa gcttttttta atttcaaatc tggggaagac tctttttcgc tatttcaaat
    51601 caagattgct ttcacttgag aaaactgatt gacgacacca actgtacttt tgttagaaac
    51661 gttctctttt ttttcttgaa acgtgatccc tcctgcgacc acaccagcca gctctttttc
    51721 tggcgctttc agtgaaagtg tctcagtgac cgcactttga ggaggaacag ttatttctcc
    51781 cactgttttt gctaagtttt tcatatcata acttaatgtt tcatcaaatt tactttttga
    51841 ttctccatat tcgatcacac cgtttgaatt cgtcgtagcg gagtggatca atgtctcaac
    51901 tgttactgat ttatctgttc ggttaaagac cgtgtagggg atttcctgtg ttttcccagg
    51961 ctccatcctt acatttatat atgttttgtt cttatcaatc tgattagccg gtaattcaaa
    52021 atctaccgca aaattgaatt cggaggcatg gacaggtgtc tgccaaagga tgaatccaag
    52081 gagaaggatc ataagtaaag agcagttgtt tcgatgactt ttattccata ttcgattcat
    52141 caatcatcct ccttgatctt tattctgtcg gcgcatctgc taatgtccat gttatctctg
    52201 ctgagtaatt accagctatc tgattttctt tggttaagac aagtttaaac gcccgaccgt
    52261 tatttaaata ttctgaataa tctgcttgca cattcgttga attttgtcct ctgacaattt
    52321 ccagttcttc ttcattcact aattgatact cctctccttt cttgaaatat aggacatcct
    52381 gcatagaaaa tcctgcatca tttgtgaaag gctgagataa ttttatcgtt aagatccacg
    52441 gatttttcgt ttctcgctga tcataaatcc ataatttttc ctgtatatcc atttggccaa
    52501 atgcttcttg tgtcaaacca gttgctttga tctgaccaaa atcgagtttg gaaggggcaa
    52561 gaaatgagag accaccttct actacgatct tcgtagcagg tgcaaaaggc ggggcatcat
    52621 gataaacaac tggcgtatca cttggattgt gattcccaat ttcatcgttg gtacttggct
    52681 tgttctgcac acccttagca gtagcttctc ctgcttggtc gtttaataca atttctaata
    52741 catctcctgc aaaaagttga ttttctggga tagaaagaac aaattcccct gtatcatcaa
    52801 tagttgtttc tgacaaaaca gcttgatttt ttttcaatga aaccaaggct cctgcttcac
    52861 ctgtacctgt gatcgtgcga gaagctgcat tgatcaatgt ggtacttaaa ttagcaggtt
    52921 caggaggaat cacatcgact gtcgttttgg cagagtccag ttctgtcact tcttggccac
    52981 ctcttacttt tttagcagca acaacttgca cttgatcctc cgtttttaat ctctcatcat
    53041 tgggtacttg aacttcgaac aagccgcctt gttcgccttc tccccatggg ttatatcctg
    53101 ccccttcatt ttcatctgtt tgtccttgcg tgagagcagt aatttctaca gatgatttcg
    53161 tcgtatctgc gtaagtaata cgtaaagtca cttctacttc gcctgtccaa gcatctcttg
    53221 gctttgcctc gacaccttcg ggaacaacga catgcccata gattttttta tctgcattcg
    53281 ttggcactct taaggattca atcatcggga gctgattatt ggcactgatt cgattataat
    53341 ttttcattcc ggcacctgtc tgagcaaatt catttgttaa acctgcacta gggcttgata
    53401 cgaaattgcc taagtcggtc ccattgatcg ataagtctgt catatcagaa aaataatccg
    53461 ggtctttttc aaagttatcg aatgttgcgc ctgtttttcg ccataaagcg atttctgaat
    53521 gggcgatctt taaagaagaa tctccatttc cttggaatac ccagccgcct atgccattgg
    53581 tctcgcttcc aatccgtttg ttttggaaat caaaataggt aggagattct aagtccacat
    53641 tcaacacatc cccactgatg atgccggtct tatcttcccc tgttttcccg accatgacta
    53701 aataagtttt ttcacctgcc gtaaaattta aatcaatatc tccagttgta tctacagttg
    53761 ctccggaatc tgcgcgtata tcgatatttg atttttctcc agtgactgta aaatcagctg
    53821 aacctggact ttctagaaaa ccagaagcgt cataaaattg gatcgcttga tttccttgat
    53881 tgagtctgtt cacgtaagat ttgccatcac ctgtatttct taccctcaat gagcctccat
    53941 tggttactgt cagcttttgc gtaccattct caaaacgaac tgcgctggaa cgcccactct
    54001 gcttatcgat gtcgataact gatccggctc cttctacgat caaagaaagg ccttttccta
    54061 caaaacgcat cccattgttc ccgggttgat cgtcatctcc agccattttg accgagattt
    54121 gtccaccact tgatacatgg atttgactag aatctccttg caagcgaaat ccgcccgtag
    54181 ttgctgtatt gatggtgatt ttcccaccat tcgatacagt gactttcgat cctgaacctc
    54241 tcatttggat gattccgcga ttgttgtcat tggtaatatt gatctttgta tttaccgaaa
    54301 attcgcttcc cgaaccactg acttcaaaca tgacattacc aggtagcgta tcgactgcct
    54361 ctccttcatc gctaacgcca tctgcccgat cataatcaag agagaccttt gaaccgctta
    54421 agatatggat tcctggcgct gtttttccac taccagtctc atctcctgaa tctagaaaac
    54481 cgtagaattt ccctagatac ggtccataaa gttgactacc attgatcaca ttcaattgat
    54541 agcttctaca aaaataggct ttacccggag cggcattcag tccttccttc actggttgga
    54601 catccaacac accggaaaca ccatctaatg tgacagttgc acgaggagcg taaatcagtc
    54661 ctaatttgga cccatcatac aaatcaagtg tcccggtaaa ttttatttct cccgtaaaat
    54721 gatatctgct tccccaaaaa tcggtcatat aagcaatgat aatggcattt ccatcgatat
    54781 ttggtgtcaa tccacctgga tgtgcagctg ttcctttaaa attctggaac tcgatcgact
    54841 gaggtatctc attgatataa atatggtgtg tgccgaaatt gtgagcgaat ccaccaaaat
    54901 caataacgat atctcgtttg atctctaatt ccctgtctaa agtcaaaggc tgaatcagtc
    54961 ggatcttagt tactgattcg tctgcgagaa gctgcgccag ttcttctgtt gtggcagctt
    55021 cgctgtaccc atccgctgaa acagctcttg ccagtgccgc ttcactgcta tcagacgttt
    55081 gttcagctac ttctgaggtc tcacttgtct tattttcttc ttgcctttta ttagtagaag
    55141 cctcactttg agattctgta gtttcagaag taatcgattc ttctgaaaaa gtagtcttgt
    55201 caaaactttg attctctgat tgcgttgatt cgtttgtttt ctctacatcg gcaaaaacgg
    55261 atgtcaccat caagagaggg aagacaaaca aaatcgacgc aatcagcttt tttacacatt
    55321 gattcattct ccaccatcct ctctgacgtt ttgacaattt tattttatcg ttttaatcta
    55381 tttccaattg tactttcata tttaaaatat gtatttttat ggaaaaattt ttctattagg
    55441 tgtaaatttc ttttttgtac aaaaaaaagg ctgtgacaaa agtcttgtca cagtccctta
    55501 tcccgaacaa gcggtgttct cccgccaact cctcggtcac aacctccatt ttaacaataa
    55561 acctgtaaat gtgagggcaa tactttgatt gtcacaggta atttatcccc ttcatctcca
    55621 tcaacattcg tcgtcaattc ttcctcatca gctaatgaca caactaattg ctcacaagta
    55681 aggtaggcta gatttttcgt agattcatct actcctttta ataggtctgg caatgtttta
    55741 agcgtatcga tcaaagaaga atcttttaaa taaaccaaat ggagcttacc atcatccact
    55801 tttgcatttg gaagtaatgt ttcaaaaccg ccgactgagt tagttagtcc tatcagcaag
    55861 gtactgctct caatttcttc cttctttcca tcaacttcta cgtgaaacga gtaggattgt
    55921 gtggaagcta actgtttgat tcctgacatg aaataagcta gttttccaaa tttcgtcttc
    55981 ttctccgcat ctacatcatt gattgattca gggatagagc caattgcgac gacattcata
    56041 aaatagtcgt catttatttt tccaatatcc aatggcttga ctgattcaat cgaaaaatga
    56101 ttgatcgctt cctcaggttc tagcggtatc cctaatgctc gagccaagtc attcaccgta
    56161 cccaatggga aaaatccgaa atttggccga tgttcttgtt cagcgattcc actgattcct
    56221 tcgttgactg ttccatctcc gcccatgaca aacacactat gatacccttc cgtagccgct
    56281 tcgcgagtaa agttttttgc atcccctgct ttttttgtat gcaagacgac cacttcatca
    56341 aacaccgatt ccaattttgc tatcgctaat tgctcaaatt cctttgcttg ttcaccgcct
    56401 gaactggggt ttaccactaa taaaactttt ttcatcactt tctttccctt ctgattgatt
    56461 tctttaatat aacgatagca taaagaaaaa aattaggctg tgatgaagta ctgttcatga
    56521 aacattcatt tattttttga caagataaaa catccatact atgaaactga ggaaaattaa
    56581 aacaatgata taggaaataa aagaaaatga taggcattgt atacctaata aagtaaaaat
    56641 cttaggtggc agataaaaac acaaaaatag caagagaatg taatagcgtt tcttccactt
    56701 tttgttatca gaccttatag cgatcagagg aataccaaat gcataactga aaaactcttg
    56761 atgtactgtt aatatgtcac ggccttgaat aataaatgcg acaatccccg ctataattag
    56821 tgtgcctaac accaaaacgg aaaattgatg aatatatttt atatacaaat gatccattgt
    56881 acttcctgcc tttttatttg tgagaaaaac aaatccgtat acgtgaataa cttctagaaa
    56941 taaaagaaat ttgcaaaacc actccattta tctacttcta cgtgcatttt ttgatattgt
    57001 tttctttatt taagattata cagaaaaata tctagggtcg ctggattcta agtaaaaaaa
    57061 atttttaaaa taaaaagaac taaatcaagt agtaat
//
LOCUS       DACDIE010000008        54322 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.008, whole genome shotgun sequence.
ACCESSION   DACDIE010000008 DACDIE010000000
VERSION     DACDIE010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 54322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54322
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.008"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            3..371
                     /locus_tag="IV250_000614"
     CDS             3..371
                     /locus_tag="IV250_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP8767921.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            551..1492
                     /locus_tag="IV250_000615"
     CDS             551..1492
                     /locus_tag="IV250_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAP8767922.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1561..2292
                     /locus_tag="IV250_000616"
     CDS             1561..2292
                     /locus_tag="IV250_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAP8767923.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2436..4616
                     /locus_tag="IV250_000617"
     CDS             2436..4616
                     /locus_tag="IV250_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAP8767924.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4636..5118
                     /locus_tag="IV250_000618"
     CDS             4636..5118
                     /locus_tag="IV250_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAP8767925.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5134..5895
                     /locus_tag="IV250_000619"
     CDS             5134..5895
                     /locus_tag="IV250_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP8767926.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6026..7360
                     /locus_tag="IV250_000620"
     CDS             6026..7360
                     /locus_tag="IV250_000620"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAP8767927.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7667..8152
                     /locus_tag="IV250_000621"
     CDS             7667..8152
                     /locus_tag="IV250_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HAP8767928.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8145..8828
                     /locus_tag="IV250_000622"
     CDS             8145..8828
                     /locus_tag="IV250_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAP8767929.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            8954..11599
                     /gene="polA"
                     /locus_tag="IV250_000623"
     CDS             8954..11599
                     /gene="polA"
                     /locus_tag="IV250_000623"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAP8767930.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11644..12480
                     /gene="mutM"
                     /locus_tag="IV250_000624"
     CDS             11644..12480
                     /gene="mutM"
                     /locus_tag="IV250_000624"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HAP8767931.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12444..13073
                     /locus_tag="IV250_000625"
     CDS             12444..13073
                     /locus_tag="IV250_000625"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAP8767932.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            13125..13457
                     /locus_tag="IV250_000626"
     CDS             13125..13457
                     /locus_tag="IV250_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP8767933.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13769..14260
                     /gene="nrdR"
                     /locus_tag="IV250_000627"
     CDS             13769..14260
                     /gene="nrdR"
                     /locus_tag="IV250_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAP8767934.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14283..15656
                     /locus_tag="IV250_000628"
     CDS             14283..15656
                     /locus_tag="IV250_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation and membrane attachment"
                     /protein_id="HAP8767935.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15656..16582
                     /gene="dnaI"
                     /locus_tag="IV250_000629"
     CDS             15656..16582
                     /gene="dnaI"
                     /locus_tag="IV250_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HAP8767936.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16711..17151
                     /locus_tag="IV250_000630"
     CDS             16711..17151
                     /locus_tag="IV250_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP8767937.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            17295..17996
                     /locus_tag="IV250_000631"
     CDS             17295..17996
                     /locus_tag="IV250_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HAP8767938.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            17993..19114
                     /locus_tag="IV250_000632"
     CDS             17993..19114
                     /locus_tag="IV250_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAP8767939.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            19129..20361
                     /gene="pepT"
                     /locus_tag="IV250_000633"
     CDS             19129..20361
                     /gene="pepT"
                     /locus_tag="IV250_000633"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAP8767940.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            20493..21401
                     /locus_tag="IV250_000634"
     CDS             20493..21401
                     /locus_tag="IV250_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAP8767941.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            21436..21708
                     /locus_tag="IV250_000635"
     CDS             21436..21708
                     /locus_tag="IV250_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAP8767942.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            21719..22765
                     /locus_tag="IV250_000636"
     CDS             21719..22765
                     /locus_tag="IV250_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAP8767943.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            23016..25625
                     /gene="clpB"
                     /locus_tag="IV250_000637"
     CDS             23016..25625
                     /gene="clpB"
                     /locus_tag="IV250_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAP8767944.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(25776..26447)
                     /locus_tag="IV250_000638"
     CDS             complement(25776..26447)
                     /locus_tag="IV250_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8767945.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(26440..26934)
                     /locus_tag="IV250_000639"
     CDS             complement(26440..26934)
                     /locus_tag="IV250_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAP8767946.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(26954..28174)
                     /locus_tag="IV250_000640"
     CDS             complement(26954..28174)
                     /locus_tag="IV250_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8767947.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            28321..30156
                     /gene="lepA"
                     /locus_tag="IV250_000641"
     CDS             28321..30156
                     /gene="lepA"
                     /locus_tag="IV250_000641"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAP8767948.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            30352..31263
                     /locus_tag="IV250_000642"
     CDS             30352..31263
                     /locus_tag="IV250_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8767949.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNRIIQYAYIGNLSLEFLIYGI"
     gene            32259..32510
                     /locus_tag="IV250_000643"
     CDS             32259..32510
                     /locus_tag="IV250_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8767950.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            32593..32865
                     /locus_tag="IV250_000644"
     CDS             32593..32865
                     /locus_tag="IV250_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8767951.1"
                     /translation="MSDGFEQVSIIKNQVREILRKKGYLVDSYFEGDYETWVGVYARP
                     ENKPTYLDPTTSEDAYLQNRYRVDGFKQDFAEWFEWEIENGEVKEE"
     gene            32955..33194
                     /locus_tag="IV250_000645"
     CDS             32955..33194
                     /locus_tag="IV250_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8767952.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            33341..37624
                     /locus_tag="IV250_000646"
     CDS             33341..37624
                     /locus_tag="IV250_000646"
                     /inference="COORDINATES: protein
                     motif:HMM:NF019941.1,HMM:NF037291.1,HMM:NF037639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HAP8767953.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            37723..38088
                     /locus_tag="IV250_000647"
     CDS             37723..38088
                     /locus_tag="IV250_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8767954.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            38090..38542
                     /locus_tag="IV250_000648"
     CDS             38090..38542
                     /locus_tag="IV250_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8767955.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            39156..40466
                     /locus_tag="IV250_000649"
     CDS             39156..40466
                     /locus_tag="IV250_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HAP8767956.1"
                     /translation="MYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLV
                     RMLIDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGH
                     LLSEVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPN
                     MLIAGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEE
                     MIICLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDME
                     ARNRVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYL
                     MTFGEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYD
                     FTVKEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            40489..40800
                     /locus_tag="IV250_000650"
     CDS             40489..40800
                     /locus_tag="IV250_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8767957.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            41161..42417
                     /locus_tag="IV250_000651"
     CDS             41161..42417
                     /locus_tag="IV250_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP8767958.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            42414..42959
                     /locus_tag="IV250_000652"
     CDS             42414..42959
                     /locus_tag="IV250_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8767959.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            42960..43460
                     /locus_tag="IV250_000653"
     CDS             42960..43460
                     /locus_tag="IV250_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8767960.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            43541..44097
                     /locus_tag="IV250_000654"
                     /pseudo
     CDS             43541..44097
                     /locus_tag="IV250_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353559.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            44117..44461
                     /locus_tag="IV250_000655"
     CDS             44117..44461
                     /locus_tag="IV250_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8767961.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            44480..45391
                     /locus_tag="IV250_000656"
     CDS             44480..45391
                     /locus_tag="IV250_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8767962.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVPAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            45470..46462
                     /locus_tag="IV250_000657"
     CDS             45470..46462
                     /locus_tag="IV250_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8767963.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            46459..46677
                     /locus_tag="IV250_000658"
     CDS             46459..46677
                     /locus_tag="IV250_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8767964.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            46690..47100
                     /locus_tag="IV250_000659"
     CDS             46690..47100
                     /locus_tag="IV250_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8767965.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            47152..49680
                     /locus_tag="IV250_000660"
     CDS             47152..49680
                     /locus_tag="IV250_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAP8767966.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            49696..51906
                     /locus_tag="IV250_000661"
     CDS             49696..51906
                     /locus_tag="IV250_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8767967.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            51914..53005
                     /locus_tag="IV250_000662"
     CDS             51914..53005
                     /locus_tag="IV250_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAP8767968.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            53026..53568
                     /locus_tag="IV250_000663"
     CDS             53026..53568
                     /locus_tag="IV250_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8767969.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VDQEAQKIDAIKIYYVRSAY"
     gene            53790..54020
                     /locus_tag="IV250_000664"
     CDS             53790..54020
                     /locus_tag="IV250_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8767970.1"
                     /translation="MCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQKGYPYGPTN
                     LKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            54034..>54322
                     /locus_tag="IV250_000665"
     CDS             54034..>54322
                     /locus_tag="IV250_000665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8767971.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSER"
BASE COUNT        18455 a         8896 c        11700 g        15271 t
ORIGIN      
        1 agatgtttac gaatgaaata aaaatcatgc tttatgtgga cgatgtagaa aaaaatgcaa
       61 cgttttggag agcagttggc tttgcggaaa aagatcgtca agaaatggat gggacgctta
      121 taattgaagt aggagttgag gacagtcaaa cctcatttct gttatatgat cgagcatttg
      181 ttgagtccca ttcacctggc acagctacta gtgccccatc tttgatgttt tacagtaaag
      241 atatctttgg actatataaa aaaatgcaga cgcaatcagt cacattaggt gatatggtac
      301 agcttggaga agaatatgtc tttaactttg ctgatcccga tggcaactac tttgcggtta
      361 caggtaaata atagaaataa agactttgtt tttctgtgcg aaacaggaaa gcgaagtctt
      421 tttaattcaa ataataatat tatgtaaaca aaaaactttc catgcgtaat tatacacaag
      481 caaataaatc gtcaaatgcg aacgccttct ttatactgtt taggaacgaa aagaaggaag
      541 gaagaaatac atggattatc aagtactgct ttattacaat tatacaaaaa tcgaagaacc
      601 agaggaattt gccaaaaatc atttagcctt ttgcaaatct ttggatctta aaggtcgtat
      661 tctagtagct gaagaaggaa taaatggtac tgtgtctgga accgttgaaa acacgactgc
      721 ctatatgaaa gcaatgaaag ctgatcctcg atttgctgat acgatgttca agatcgatcc
      781 aagcgaagga catgccttta agaagatgtt tgtaagatac cgtccagagc ttgtaagttt
      841 aaagctagaa gaggacattg atccaaacca agtgacaggt gcctatcttt caccgcaaga
      901 atttaaagaa gctattttag atgaacagac agtggtgatt gatgcacgga atgattatga
      961 atatgacttg ggacattttc gaggggctgt acgcccagat atacgcagtt ttcgagagct
     1021 tccagactgg atccgcgagc acaaagaaga atttatggaa aaacgggtag tgacctactg
     1081 tactggtggc attcgttgcg agaagttttc tggttggctt gtccgtgaag gattcaaaga
     1141 tgtcggacag ctgcatggcg gtattgctac ttatggtaag gatcctgaag tccaaggaga
     1201 attatgggac ggcaagatgt atgtgtttga tgaacggatc gctgtcgata tcaaccaagt
     1261 agataaacaa atcgtcggtc gcgactggtt tgacggaaca ccatgtgaac gatatatcaa
     1321 ttgtagcaat cctgagtgca atcgtcagat tcttgcttcc attgaaaatg aagaaaaaca
     1381 tttaggagca tgtagcatag agtgtgcgaa acatccgcgc aatcgctatg ttcaagacca
     1441 tggaatctct ccagaagaag cagaactgcg gtttacagct ttaattaaat agaaaacaat
     1501 agttttgatt gtcgaccatt tccaatatgc gctatactag cgttgaggat ggtgatccac
     1561 atgaaatcat tgatttatgc tattggggat gtccatggca aacatgagct ttttgaaaaa
     1621 cttattcact attatgatcc aaagatccat cagcttgttt tattagggga tctcaatgat
     1681 cgaggtcctc gtacaaaaga atgtttttta ttaggaaagc aattagtcga agaaacagga
     1741 gccatttacc tccggggaaa ccatgaagaa tattttttgc aatttctgca atcaccggag
     1801 gactggtatg cgccttatat gagaaatggt ggaaaagaaa cgtttgaaag cctgattcac
     1861 ccaggggcat cggaggaata ctccccaact gaaatctcga tgatgatcca atcgagatat
     1921 tcagaactga ttaaattttt agtccagcgc cccttgtatt atgaatggga aaattatctt
     1981 tttgtccatg ccggtgtgga tttgacaaag aaagactggc gtaagacaag tctaagagac
     2041 tttatttgga taagggaacc atttcatgaa gggaaaaaca atacaggtaa gacaattgta
     2101 tttggacata cgattacacc aatgctccat ggagatatgc agacaacaga cctctggatt
     2161 caggatcata aaatcggaat ggacggtggg gctgtattcg gagggtcatt gcatggggtt
     2221 gtttttgaca aggaaggtat tcttcaagat attgaatatc agaacaacga aggaccttgg
     2281 catggaatct gactaatgga aagaatgaag gagctgtgac tttgttgcgg ctctttttat
     2341 ttttatagag aatattgttt ctttcagtag tttaacccag gataaaaagt atgatagaat
     2401 aaagaagcaa tttttagcag aaaagaggca ttttaatgac ggaaaatttg aaccagacga
     2461 tcattcagct tgtacaaaaa gaactttcag actatcgtcc taagcagttg actactgtac
     2521 ttaatttgtt aaatgaagga aacactgttc cttttatcgc gcgttaccga aaagaaatga
     2581 ctggaagtct tgatgaagtc cagattcgtg aaatcgaaga gcggtatgcc tatcttgaaa
     2641 acttagaaaa acgtaaaact gaagtgattc ggctaatcga tgaacaaggg aaactgactc
     2701 ccgagttaga agcagaaatc acccaagccg tgaaaatgca acaagtggaa gatttgtatc
     2761 gtccatacaa acaaaaacga cggacaaaag caacgattgc taaagaaaaa ggacttgaac
     2821 cactcgcgaa gtggctgatg cagcttacag atggagaggt gcaatctgaa gcagaaaaat
     2881 atattgacaa agaaaaagaa gtctcttctg ccgaagaagc gttgcacggt gctcatgaga
     2941 ttattgctga acaagtcagc gataatgcaa aatttcggac ttggattcgg tcttatacgt
     3001 ataacaaagg tatgtatgtc agcaatgtca aggatgagca agcagatgaa aaaggcgtat
     3061 atgaaatgta ttatgacttt gcagaacccg tccataaaat ggtttctcat cgaatattag
     3121 ctactaatcg tggagaaaaa gaagatattc tgaaagtatt ctttcaagta gatgaagcag
     3181 caattttagc ttacttggat cgccaaatag tcaaaaatcc tgcctctcct tcttcttcat
     3241 tcgtcagaga agcctatcag gatagttata aacgctttat tcagccagca atcgagcgag
     3301 aactgcgcaa tgaattgact gaaaaggcag atgagcaagc aatcgctatt ttcggcgaaa
     3361 acctccgtaa tcttttgttg caaccgccac taaaaggtaa agttgtcctg ggatttgatc
     3421 cggcttatcg tactggatgt aaactagcag tcgtagatgc gacagggaaa gttttagcaa
     3481 tcgaagttat ttatcctcat aaacctgcag cgcaagcaaa aagagaagct gctggaccag
     3541 cttttatcca attaattaat aaatatcaag tagatatggt agctatcgga aatggtacag
     3601 ccagtcggga atctgaactt tttgtggctg agcagttgaa atccgccgac cataaaacct
     3661 attacgctat cgtcaacgaa gcaggcgctt ctgtttactc cgctagcgaa attgcacgaa
     3721 aagaatttcc gcatctacaa gttgaagaaa gaagtgctgt ttctattgcc cgtcgcctgc
     3781 aagatccttt agctgaatta gtcaaaatcg atcctaaagc tgtcggagta ggccagtacc
     3841 aacatgacgt ttctcaaaaa cgtttggctg aacaattgga ttttgttgtg gaaacagcgg
     3901 ttaaccaagt aggtgtggat gtgaatacag caagcccgca gctcttgcag catatatcag
     3961 gattgaataa aacaactgcc caaaacattg tcatttatcg tgaagaaaac ggagaattca
     4021 ccgcacgaac tcaattgaaa aaagtgccac gcttaggacc aaaagcatat gaacaagcaa
     4081 tcggattctt gcgagtccct ggtgggaaaa acattttgga caacacaggt atccatcccg
     4141 aaagttattc tattgcaaaa gaaatattaa cttcagtcca actttcagaa aaagagcttg
     4201 gtacagaaga agcagtagaa aaattgacac gattatccgt agagaaactg gctgaaagcc
     4261 tatcagtcgg agaagaaacg ttagccgata ttttagctgg gctgacacaa cctggacgag
     4321 atatgcgtga tgagatgcca gcgcccttat tacgtacaga tgtattgagt atggaagact
     4381 tgaaaccagg gatggaattg actggaacgg tccgtaatgt cattgacttc ggcgcatttg
     4441 ttgatatcgg tgtgaagcaa gacggacttg tccatatatc gaaactcagc aaaaaattcg
     4501 tcaagcatcc tacagatgtc gtgtcagtag gagatatcgt aacagtttgg atagaacaag
     4561 tcgatacgaa aaaaggtaga atcagtttga cgatgctttc cccttatgaa gagtaacaat
     4621 gaggagtaac aaaaattgac acaagaagaa ctgcagcagt tagtagaaga gatatcatta
     4681 aaaagcttcc agcttccttt caagcaccaa gcaatgttca atccacgctt acgtacaact
     4741 ggcgggcgct atcatttaaa cgatcatcat ttagatttta atgcgaagct atttgaagag
     4801 tcagacgaag agacgattgc aggaatcatc aaacatgaac tttgccatta tcatcttcat
     4861 ctagctggaa gagggtatca gcataaagat atggatttca agtatctttt gaaaaaaaca
     4921 ggagggttgc gctatgcacc agccttgacg aagaaacaga taaagatgga gtattatcga
     4981 tgccaagatt gttctgccac gatctgccgc aagagaagaa tcgatgtacg caaatatcgt
     5041 tgtggaagat gtcatggaga aatcaaatgg attgagacaa aaagagaaga ctttaatgaa
     5101 gcacaaaaaa tcagatgaaa ggatagaagg cctttggaaa aaagtgagca gaggaatcta
     5161 aaagactgga atcagctatt aaatttttta gccgcacctg aagagggaaa ttttgaggaa
     5221 ctgacggcac ctacacttat tttagctgga aactgtcttc ctatcttagc tgataaagca
     5281 gcacaaatgt atttaaatgg ccaagtagat caattgtttt tggtcggagg ggtggggcat
     5341 gcgactagaa tcctttatga gaattttgaa aagcaaggat ttcattttga agatggcatg
     5401 agtgaaagtg aaatctgtcg tcagtatcta aaagaagtct atgatttgcc cgataaggct
     5461 tttctgatag aatcgaagtc tacaaattct ggagagaacg ccactttttc actggagatt
     5521 cttcattcct taggagctgt accagaaaaa atcttattga tgaatgatcc gacactgcaa
     5581 cgcaggactc gagcaacttt tgagaaagta tggcaaaatg aacaaactat tttcgcgaat
     5641 gctgtgccat ttgtgccgga aatccttcac ttttctgaag agatcatctt tacagcaaaa
     5701 gaactgaatc atcaatggcc taaggaatat ttccatgcgc tagtattagg agaaatggaa
     5761 cgtctccatg acgatgagaa cggctatggt ccgaaaggaa aagattttat tcctcacatt
     5821 gatatatcag aggaagtttg gtcttcttac accagaatta aagagtcaat aaaaacggat
     5881 ttttctcgta cttgaccatt ttagcaagcg aatcctcctt tccaaataaa tttggttgaa
     5941 attttcaatc gtttataaga taatgaggag cgtgttgaat gtaatagcta aaagaatgga
     6001 cgctagataa gagaagagga ttcaaatgga aaatcaaaat aaattgtatc attttgttgg
     6061 tatcaaaggt tcaggaatga gctccttggc tcttgtttta catgaacaag gtctaaatgt
     6121 ccaaggatca gacattgaaa aatatttctt tacacaaaga gatttagaaa aagcaaatat
     6181 cacgattctg ccatttaacg cagataatgt aaaaccaggg atgacgatca ttgcaggaaa
     6241 tgcatttcct gattcacatg aagaaatcca gcgagcaaaa gaattaggtt tggaagtgat
     6301 tcgctatcac gactttattg gtcattttat ccaaaattat acgagtatcg ctgtaacagg
     6361 ctctcacgga aaaacaagta cgactggact gctctctcat gtattgagtg gtgtccgtcc
     6421 aacaagttat ctaattggag atggaacagg acatggtgat ccgcaagcgg aattcttttc
     6481 atttgaggcc tgtgaatatc gccgtcattt ccttgcctat tcaccagact atgcaatcat
     6541 gaccaatatc gattttgatc atccggatta ttatacaagt atcgacgatg tatttacagc
     6601 tttccaaaca atggctggac aagtgaaaaa agctattttt gcgtatggtg acgatgctta
     6661 tttacgcaaa ttaaaagcaa atgtgccgat ttattattat ggtgtcacag aaaacgatga
     6721 tatccaagca cgaaacatcg aacgcacaac ttctggttct gcttttgatg tctatcatgg
     6781 ggatgaattt gttgggcact tcactgtacc agcattcggt aaacacaata tcttaaatgc
     6841 tttaggtgtt attgcagtag cttatttcga aaaactggat ctgaaagaag ttgcagaaga
     6901 aatgctgact ttcccaggag taaaacgtcg attcagtgaa aagatcgtag cagacatgac
     6961 cgtagtcgat gattatgcgc atcaccctgc agagatcaaa gcaacgattg atggcgcacg
     7021 ccaaaaatat ccagataaag aaataattgc agttttccaa ccccatacgt ttacgcgtac
     7081 gattgcattg atggatgaat ttgcagaagc acttgattta gctgataaag tttacttatg
     7141 cgatattttt ggttcggctc gggaagaaca aggaaacgtt aagattgaag accttggagc
     7201 gaaaatcaaa aaaggtggag aagtcatcaa agaaaataat gtatctccat tgttagatta
     7261 tcatgatgca gtagtaatct tcatgggtgc tggagatgtt caaaaatttg aacaagcgta
     7321 tgaaaaactt ttaagcagca cgacgaaaaa tgtactgtaa tacaattact gcagtgtcct
     7381 tataaaaata gaaagagggg gagtggcaaa aatcttgcca agcccccttt tttgcaactt
     7441 atgataaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaatcaa atcaaaaaca
     7501 tgagaagctg ttttttcttg gctgttctta ttatttgatg caggacagct ttttcacgac
     7561 ctctgtaaac tataggaatc tgttaaatca agggagcaga acttgccttt tacgctactt
     7621 ttggtacaat aatgtatgat taaaaataaa ctggagggtt tctatcttga acataggtaa
     7681 gccgagaaaa ctaggaaaaa caataaatga aatcgaggaa ggcgactcgt tatccttaac
     7741 agaatctatt gaagataacc agcttttgct gtatttggga ttgacgaatg atgcaaatcc
     7801 tttgtatatc caacatgact ttgcacaaaa aacagaatat caacagccaa tcgttccatc
     7861 agtgatgctg atggggatca tcacaagtgc aatttcaaag catcttcctg gaccaggatc
     7921 acatgttgtc aatttttctg tcaattttat tgaaccggtt ttccattatg aaacactgac
     7981 ttttcagttt gaggtcataa aagtagataa gatgaaggat gtggtgacga tttctgtaga
     8041 agcggagaat acgaacgaag atcgtgtgtt ggatgctgtc gttatggtcc aaccgccaca
     8101 agttcctgaa ttggaaatag caatcgaaag tgaaggtgaa ttgaatgaat aatccaagta
     8161 catcaatgtc aggtatcaat cgtttttatg caaaagttta tggattttta ggtatgggac
     8221 ttgcccttag tgcagtcact tcttacttgg ctctttgggt atttcctttg caattagcaa
     8281 cttttatcaa taattttcca ctaggctttg tgggtctgtg gatcgttgaa ctgattttag
     8341 taatggttct aggcgtaaaa gcacaaaaga atccgagctt agcaatcggt ggatttattg
     8401 cctactctct tttgaatggc ataacattag cggttactct tgcatactac gatattggaa
     8461 cagtaacgca agctttcttg acagctactt tgatgttcat ggggatgtct gttgtcggag
     8521 taacaacaaa aagagatctt agcggggttg gaagagcagg acaggcagct ttaatcggag
     8581 tgatcattgc actagtcttg aacatcttca ttttgagaag tagcgtagtt gagctgttca
     8641 tctccctact gttagtcgtc atctttgcag ggattacagc ttatgataac caaagcatcc
     8701 gtaatatcta tgtccagaca aacggaacag cacatagtgg aattgctgta ttcatggcat
     8761 tgaagctgta tttggacttt atcaacttgt tcttggcgtt tctgagaatc tttggacgga
     8821 acaactaaaa aagaaaaaaa ttcatcagtt ggggctatac cgaattattt ggtatggtcc
     8881 cgctttttct tttcgcattc cgcaggattc tttgttagaa tgaataagaa ctttacagta
     8941 aggagttaag aagatgacaa aaaataaatt attactcgtt gatgggaaca gtgtggcttt
     9001 tcgcgcattc tttgcgcttc acaattctct tgaacgtttc aaaaatagaa acggtcttca
     9061 tacaaatgca atctatgcat tcaatacgat gtttgaaaat gttatgcaaa aagagcaacc
     9121 gactcatgtt ctggttgcgt tcgatgcagg aaaaacgact tttcggacag aaatgtatgc
     9181 ggagtacaaa ggtggaagat ccaaaacacc tggcgaattc aaagaacaaa tgccatatat
     9241 acgtgatttg ttgacaggac taggggtaca gtattacgaa ttgcccaatt atgaagcaga
     9301 tgatattatc ggtactttgg cagaaaaagt agacaaagat caatttgatg tcgtcgtgct
     9361 ttcaggtgat cgggatttga ctcaattagc tagtaaagaa gtaaaagtgg atatcactgt
     9421 aaaaggtgtc agcgatatcg aaagctatac accagaacat gtagcggaaa aatatgatgg
     9481 gttgacgcca aaacagatta ttgacatgaa gggactagcc ggagatgcct ctgacaatat
     9541 ccccggtgtg actaaaatcg gtgaaaaaac agctatcaag ctattgaaac aatatggctc
     9601 agtcgaagga atttatgaac atatcgatga aatgaagcaa agcaaaatga aagaaaatct
     9661 gataaatgac aaagagcagg ctttcttatc gaaaaaattg gcgaccatca atacttcagc
     9721 gcctgtggat gtctctatcg actcattgaa gtatgaaggg aaaaatctcg ataaactcgt
     9781 tccgttttat aaagaaatgg atttcaatca gttcctttct aaattgaata tcgtagaaga
     9841 agaagtaaaa atggatgata ttttgtttga agtcgttcat gaattcaaag aggaaatgtt
     9901 tactacggat atggcgttgt acgtggaaat gatgggcgac aattatcata cagaggaaat
     9961 cgtcggagta gcgtggggga cagaaaagaa aatttatgtc acgaacgatc tatctgtatt
    10021 cgaaaaccga gcgttccatt cttggatcac tgatccgact cggctgaaaa aagtatatga
    10081 tgcgaaacga acttatgttg ctttaaaccg ctatactgga aaaacgaaag gaatcgattt
    10141 tgatgtcttg ctaggtgcat accttctcga tacgaatgaa aaaagcacag atatcgaagg
    10201 aatagctgct cattacggat acaacgatat ccaatcagat gaagctgtat acggcaaagg
    10261 ggcgaaaaaa ggtttaccag aagaagagga actctttttt gctcatttag cgcgaaaagt
    10321 agctgcaatc aatgcgttga caccaaaatt gagccaagaa ctggcagaaa aaaaccaaga
    10381 agatcttttc cggaaaatgg aactgccatt gtctatcatt ttggcagaga tggaaataag
    10441 cgggatcaaa gtagatgcga ctcgcttaca agaaatgaag ggcgagttct ctgctcgctt
    10501 aagagagatc gaacaaaaaa tctacgaaga agccggcgaa gaattcaatt tgaattcacc
    10561 aaagcaatta ggagtgatct tatttgaaaa aatgggatta cccgttatca aaaaaacaaa
    10621 aactggatat tccacagccg tagatgtact ggaacagctt cgtgaacaag caccaatcgt
    10681 agaggatatc ctcacctatc gtcaaattgc aaaaattcaa tctacctatg tcgagggact
    10741 gctgaaagtg atccaatcag atggaaaagt gcatacccgt tatgtccaga cattgaccca
    10801 aacaggacga ttaagctctg ttgatcctaa tttgcagaac atcccgattc gattagatga
    10861 agggagaaag atacgtcagg catttgttcc tagagaaaaa gactggctga tttattcttc
    10921 tgactattca caaatcgagt tacgcgtact tgcgcatatc tcaaatgatg aacatttgaa
    10981 agctgccttc ctagaaggtc aagatatcca tgccagcaca gcgatgcgtg tatttggaat
    11041 cgaaaaggca gaggatgtca caccaaatat gcgtaggcag gcaaaagcag tgaattttgg
    11101 tatcgtgtat ggcatctctg attacggact ttcacaaaat ctaggaatca gccgaaaagc
    11161 agcacagcaa tacatcgata cgtactttga aaaataccca ggtgtcaaag aatatatgga
    11221 aagaatcgta cgcgaagcaa aagaccaagg ctacgtcgaa acgctgtatc accgccgtcg
    11281 atatctgcca gacatcaatt caagaaatta caatatccgt tcttttgcgg aacgaacagc
    11341 tatcaataca ccaatccaag gaagtgctgc tgatattttg aaaatcgcga tgatcgaatt
    11401 ggataaacga ttaaaagaaa caggtcttca agctacgatg ctgttacaag ttcatgatga
    11461 acttgtcttc gaggtaccgg aaaaagaact tgaatcatta gataaattgg tgaaggaagt
    11521 tatggaacaa gctgtttctc ttcatgtacc actgatcact gacagcagtt ggggaaaaac
    11581 gtggtacgaa gcaaaataaa cattgtttaa gacagagcaa tataatgaag gtgaaaagaa
    11641 aaaatgccag aattaccaga agtagaaacc gttcgtaaag gattggaacg gttagtagta
    11701 ggaaaaacga ttcaaaaagt tcaggtcttg tggcccaaga tcatcgagca accagagaca
    11761 cccatctttg aggcttctct tgttggcgaa accatccaat ccattggacg tagagggaag
    11821 tttttaattt ttcatctcga tcattgtgaa ctgatctcac acttgcgaat ggaaggaaaa
    11881 tatcaattta cgaaagaaaa tacaccaatc gataaacata cgcatgtact tttctttttt
    11941 gaggatggaa gccaacttcg ctacaacgat gtacgaaaat ttggtcgtat gacaatcgtc
    12001 gaaaaaggag caagtgcaac ttaccgagga atcatgaaat tgggacctga gcctttgcca
    12061 gatagcttct tattagcaga tttcgcaaat ggactgaaaa agtcccacaa agcaatcaag
    12121 ccattgctgc tagaccaacg tctagtgact ggtctaggta atatttatgt agatgaagca
    12181 ttatgggaag cgaagatcca tccagaacaa ccggcgaaca cactgcgccc caaagaggta
    12241 gaacaattac gtctatcgat cattgatgtt ttggatcgtg caatagaagc cggaggtacc
    12301 acgattcgca gttatttgaa tgcattagga gaatcaggcg gatttcaagt agcactgcat
    12361 gtctatcagc aaactggcaa accatgtata cgttgtggaa cgccgatcgt gaaaacaaaa
    12421 gtggcacaaa gggggacgca ctattgtccg aattgtcaaa ggctaaaaat cgcaaagtag
    12481 gatttgtttt aggtttgacg ggtggaatag ctaccggtaa aagtacggca gccaaagtct
    12541 ttcagtctca tggatttcct ttgatcgatg gcgatgtgat tgccagagaa actgttgagc
    12601 ctggaacgct tgctctgagg aaaattgtat cagtgtttgg ccaagaggta ttgcagcctg
    12661 atgggcaact agatcgaggg aaacttggga tgattgtttt tccatcaaag gaaaaacggc
    12721 aaaaacttga tcagctatta gatccgttta tccgaaaagc gataaaagat caaatcgcct
    12781 ctctaagttc caatcatcct ttagtaattg tagatatccc attgctctac gaaggtcatt
    12841 atgaccacta tatggatgca gtagctgtcg tttatactac gccagaaact cagcttcagc
    12901 gtttgatgaa acgtaatcaa ctgacgctag aacaagcgca gcagagaatt agtagtcaac
    12961 ttccaatcga agaaaaaaag cagcgtgcgg atattttgtt cgacaacaat ggaacaaaag
    13021 aaaaacttgt gggacaaatc gaaaattggc tttttgctaa tcgctttctt tgagtatctg
    13081 tgtcaatttt gctatcctga gtatatagaa agtgagggat agcgatggga tcattaaaaa
    13141 atcaaaagga taaagtcgta ggtaacgcaa aagaaaaaat cggcgagatg ctcaataaag
    13201 aaaatctaat cgatgaaggt cgtacgcaat cagaaacagc agaagaaaaa gaaaagcagt
    13261 accaagcaag aatcgatgaa atagaagacc agcaggcaga agaagaagcg aagcggcaag
    13321 atttgactca agaacgaaaa aatccgacac ctgatcgtag taaaacaccg ctcccagaaa
    13381 gtgaccacat tcccgatgag caaaaaatcg aggaacagcg aatgaaacac tatacaggaa
    13441 tcgaagagaa gttataaaat agtaaaaaat cgagatgtga cttgaagcag atgagaaggt
    13501 gttcatctgc ttcaagtttt ttgtgctaga tgactaaatc agcttttgtg taagttattt
    13561 ctcagtaatc aaggtgatca gtattggaaa attgaaattt agtaaggaca ggggacacaa
    13621 ttacgcatga aagtagaaaa aattgttctt atatccgtgt tttttgggta ggacagtcgc
    13681 gtttgtgatt tgtttcgtgt tataatacaa ctagttgtaa agaaagacac atcctttata
    13741 taaaatgcga tgcaagaggt gagacaaaat gagatgtccc agatgtcaac ataataattc
    13801 acgagtaatc gacagccgtc aagcagatga tggacgtgcg atccgtcgcc gtcgcgaatg
    13861 tgagaattgt tcgttccgat tcactacatt tgaacgaatt gaagctgctc cgttattagt
    13921 aatcaagaaa aacggggaac gcgaagagtt caatcgcgat aaaattttaa gaggactcat
    13981 ccgttcagca gaaaaaagac ctgtagctat ggaacaaatg gaacaaatcg tcaaccatgt
    14041 cgaaaaccgc gtacgcagtc tcggagaaaa cgaagtatcg acgaatctaa ttggtgaata
    14101 tgtgatggaa gatttagttg atttagacga aatcgcttat attcgttttg caagtgttta
    14161 tcgccagttc aaagatatgt ctgttttctt aaaagagttg caagatatcg tggataaagc
    14221 aaaagaaaat aataaagaga ataataaaga aaacaattaa gaaacaaaaa ggaggcattt
    14281 ccttggaaaa tgcttggaaa gagcttcaac caaaaaatat ttaccaagga agaaaagcgg
    14341 ctccgctgag agaagacagc aagaacgcct tgctgtatct ttatcagcca ctaatcggcg
    14401 gagaagcctt gagtatctat ttcaccctgt tatcagaaat ctctcaagaa tctggcaaag
    14461 gtcctgaagg gatgcatgca gatctgctta gtagtctagg ctgcggtatc ccgcaatttt
    14521 atgatgcaag aaaaaagcta gaaggaatcg gcttactgga tgtttatttt aaagaagata
    14581 gtgatctggg acctcgtttt atttatgagc tgttagaacc actcattcct aaagcatttt
    14641 tcaaagactc tgttctggct tttcttcttc tggaaaaagt tggtgaacga cgattccaga
    14701 agcttaccca attctttgaa cctaaaaggc atacagcaga gggatatcaa aaaatcacaa
    14761 aaaaattttt agatgtttat cgcttcaatg aagcagtgta cgctgctaat caagaaaaca
    14821 tcgaatcgct gaataaatct tttgaagaat ctgaatcagt agcattactt gaagcagatt
    14881 cgacattcga ttggcagttt ttgaatgatt ggctggcaaa aaaacatatt gcaagtcttg
    14941 atgatccaat catcaaacaa gtcgacatgt atcatcagct ttacggtttc gatgaattga
    15001 cacttggcga actgatcagt caatcttatg actttagtga acaaaaagta tctgaaaaag
    15061 aactgcaaaa agccattttg gcaatgcaga gacgtccggt gaagcaaaca aaagaaacag
    15121 ttgcggtttc tgaaaacagt aatgaaccct cgtcaaacga acatttgaca aacgcagcgc
    15181 ttcagctgat aaaagaagca cagagcattc caccgatgaa atatctggaa gcaatcaagt
    15241 cagaaaaagg cggatatgtt tctaaacaag aaaactggtt actgcaagat cttgtttctc
    15301 aatcaggtct ttccagtagc gtgatcaatg ttttgctgaa ttatgtacta gtgatcaaga
    15361 ataatgctag cttaaatgcc agttatgtaa atacagtggc aaatgaatgg gcacaaaaga
    15421 aaatcgccac agcagaagaa gcgatccagc acattcggca aataagtaat caagcaaaac
    15481 aatcaaaaca aaagaagcaa acaaattatc aaactggcaa gcgaaatgtt cgacgggaaa
    15541 agcttcctga ttgggtcaac cagccaaaag atgaaaaaca gatcagcagc gagaaaaaag
    15601 cagagattga ccgccgtttt aaagaatacc tggcaaaaaa ggagggtgat atctagtgga
    15661 agacgtaggc aaagaattga agaggatcat cagcaaacgc aattatcaag aaaagtatgc
    15721 tgaaatgatc gctgaagtct taaatgatgc ggatgttcaa gcttttctaa gcgcacacca
    15781 agaagaattg acacaagcgg atattgaaaa aagttacgct aaattgtatg aattcgtgca
    15841 agaaaagcgt aaatatcaaa aaaacgatcc aacgatgatc gctcccggct atgagccgaa
    15901 gctgacgttg aattttcatt atgtggatgt cacttatgta ccaacggaag cattgttagc
    15961 acggcaaaga caagaagaaa tcagaagccg agtaaaagca atggatatgc caaaagatat
    16021 tcaatcggct tcttttgaaa actttgaacg tacagatgga cgaggatatg cgagcttaga
    16081 agccctagat tttgtagaga aatatactgc tgatccaaaa gggtatcata aaggactgta
    16141 tctagtagga agttttggta ttggaaaaac atatttatta ggagcaacag cgagagaatt
    16201 agcgattgct ggattcacca cgacgctagt ccattttccg acatttgccg ttgagatgaa
    16261 acaggcaatc ggaaaagatc aagtggcaga aaagttagat gcagtgaaac gttcacctat
    16321 tttgatgatc gatgacattg gtgcagatgc gatgagtagt tggatacgag acgaagtatt
    16381 cggtgttatc cttcaatatc ggatgcagga acagttacca accttcttct catcgaattt
    16441 tacaatggat gaattggaac agcatttgtc cgtcactcaa cgtggagatg aagagccgct
    16501 aaaagcgaaa cggataatgg aacgcatacg ttatttgaca aaagaaatcg aaatgactgg
    16561 tcgcaaccga cgcaatggtt gaacagttga caagtccaac tgacatttga cctagtagta
    16621 aaagcaggtc agcgaatcat tatatggaca gaaatgaccg gagacaatca ccgcaacaag
    16681 taatgcagaa acttaaacgg aggatttttt atgagggcta ttttttctag tatatctgta
    16741 gttggtctat tttttctttt agcaggctgc ggtagcgcag atagcaacac aggttctgtt
    16801 gattcgattg atgcgggtca aacagtaaaa acaagcagta cgaaaacgga gagacaaagc
    16861 atatttgtcg gaacgattga aagtatcact caagtagaag cagaagaaga aacgcttcag
    16921 ttgaaactcg tcgatgtcat ggccaaagaa gatccagcga atatgggaac gtcattcagc
    16981 aatgacggag tgatcttaaa tgctactgct gaaaaaataa gcgtggacat aaatgaattg
    17041 aaaatcggtg acaaagtcca atttacatta gttgagatgc cgatcatgac gatgtctatc
    17101 ccaccgcaag ttcctggaga ttcgattatt gatgtttcat tgaacaaata accaataaca
    17161 ttttttcaaa aataagaaaa tgttgtttta tagaacgaaa aggtatattt tttaaggaga
    17221 tatgggaatt tttttattcc tagtctcctt attttatttg gaaaatgcta taattatcta
    17281 gaaggagtga agctatgaac catttagatt tatctcagcg cttagccgca gtgggtgact
    17341 ttgtccctga atccgctcgt ttagcagaca ttggatcaga tcacgcttac ttacccgttg
    17401 cattgatatt aagaggaaaa atcgaatatg cagttgctgg tgaagtcgtt caaggtcctt
    17461 atgaatcagc acgtaagcaa gtcaaaaaag atggattaga agaccgtatc accgtacgcc
    17521 ttgcaaatgg tctagatgct atccagccac aagatggtat tacagctgtc acgattgcgg
    17581 gtatgggagg ttcactgatc cgagatatcc tagaagctgg caagttgaat caacgcttaa
    17641 gcggacaaga acgtttgatc cttcagccaa acatcggaga gcgtacatta cgatcttggc
    17701 tgcaagaaaa tcagtataaa atccaatcag aaaaaatact tgaagaaaat aaaaaaatat
    17761 atgaaatcat tgtagccgaa aaagaacaaa aaccagttac ttacaatgaa atggagctga
    17821 tgttcgggcc atttctgctt gaagagaaaa gtgagatctt caaatcaaaa tggacaagag
    17881 agcttcaaca aagaaaagct atccgtgaac agctcaaaca agcagctaat caagaggcac
    17941 gtcaaaaaca aatcgagcag gaaatccaaa tgattgagga ggttttaaag ccatgacaat
    18001 cagcggacgc actttcatcc aaaagtttga aagctattgt cctcagtggc tagctgaaga
    18061 aggagaccca gtcggactgc atatcggaac attggataag ccaatccaac gagtaatgat
    18121 gacattagac gttcgtccag aagtggtcga agaagcaatc aagaaaaaaa tcgatttgct
    18181 gattgctaaa catcctccaa tctttcgtcc tgtaaaacgg ctggttactg atcagccaca
    18241 agaaaaaatg tatgcagatt tgttgaaaca tgatattgcc gtctatgcag cacacacgaa
    18301 catggatatt atttgggatg gactgaatga ctggttttgt gaacttctcg gtattgaagt
    18361 ggagagttat ttagtaaaga ctcatgagat tcattataaa aaactggccg tttatgtacc
    18421 tgtggatcat gcacagaaaa tgcgtgaagt attagctgca gctggtgctg gtacacaagg
    18481 tgattatacc ggtacaagtt ttacctcgat tggacacggt cgatttactc cggaagctgg
    18541 tgcacaacca gcgattggga aagtagggaa gacagaacaa gttcaagagg caaaagttga
    18601 ggttattttg ccggaaacta tcgaaaaaca agtgattcaa gccatgcggt cagctcatcc
    18661 ttatgaggaa cctgcttatg atcttttcgc tatagatgaa ccagtcgaaa tgtttgggtt
    18721 aggacgagta ggtgaattgc cgcaagaaat ttctatagag gcatttgtag aacaagtaaa
    18781 agaagcgttc caactagatg gactgagaat agtccagcca aaaaatgcaa aatcctctgt
    18841 gaagcgcatt gcgatttgcg gtggaagcgg tgaaaaattt tatccgcaag caatcgccca
    18901 aagagcagat gtctatatca cgggagatat ctattatcat acggctcatg atatgcaaag
    18961 cgctgggttg attgcaattg atcccggtca ttatattgaa tcgttatgca agcaacgatt
    19021 tatcgagaaa tttgaatctt ggaaacaaga agaaaattgg gatattaact ttttcgtttc
    19081 tgaaacaaat acaaacccat ttcaatttaa ttaaaaggag agaatactat gtacgaaaat
    19141 cttttgccac gttttttacg atatgtcaaa acagaaacac gctccgatgc aacaagcaaa
    19201 acgactccat cgactcagac acaagttgct tttgcgcaag aattgaaaaa agaattagaa
    19261 gagttaggct tagctgatgt tttctataat gaagataacg gattcgttat gggaacatta
    19321 ccaagcaatg ttgatcatga agtgcgttcc attggattta tttcccacat ggatacagca
    19381 gattttaatg cagtaggagt caatccacaa atcattgaaa actatgatgg cgagtctacg
    19441 atcgtactag atgcggaaga acgctataca ttgaatacaa aagattttcc aaatttgaaa
    19501 aattatgaag gccacacatt gatcacaaca gatggcagta cattactggg agcagatgac
    19561 aaatcaggga ttgcggaaat catgactgca atggagatct tgatcaataa tccttctatc
    19621 cctcacggta ccatcaaagt ggcttttggt cctgatgaag aaatcggtgt cggagcggat
    19681 aaattcgatg ttgagcaatt cgatgtagat tttgcttata cagtcgatgg tggtccttta
    19741 ggtgagttgc agtatgaaac gtttagtgct gctcaagcaa atattacgat ccaagggaaa
    19801 aatgtacatc cagggacggc aaaagataca atgatcaatg ctctacagtt agcaatcgat
    19861 tttcataatc agcttccagc cgatgaagtt ccagaaaaaa cggaaggata cgaaggtttc
    19921 ttccatttga tggcattgga tgggaatcct gaagaagctt ctatgagtta tatcattcgc
    19981 gatcatgatc gtgaaaaatt cgaagcacgc aaagcacaaa tcacacatat ccaagaagag
    20041 ttgaatcagc gatttgatca agaacgaatc aaagtagata tgtatgatca atattacaac
    20101 atgaaagaaa tcattgaaaa agatatgagc atcgtggaat tagcgaaaca agcaatgatc
    20161 gagcttggta tctctccagt gatcgaacca gttcgtggag gaacagatgg ttcaaaaatc
    20221 tcttacatgg gtattccgac gccaaatcta tttgccggag gagaaaatat gcacggacga
    20281 ttcgaatttg tttctttaca agtaatggaa aaagcaacag acgtgattgt aaaaattgca
    20341 gaattgaaca caaagagtta aaatcgaaaa agaacagctt atgacaaggc gttcgtccca
    20401 atgatggaat gtcatctgtc taaggctaaa gggcaggaca atggaattgg ttgtcccgcc
    20461 ctcttttttg aagagtaaat aggaggtaaa gaatggaaaa gctgctagtt ttttataaca
    20521 aaagttcagg gaaagatgag ggtgagaaat tagcacgctg gtttcaagat tatgtaaaag
    20581 aaaagcagcc agaattatat gtagaactag ctgaaactgg cccatcgatt gatgatgcgg
    20641 aattagtcaa gaaagcagaa gccatagaag ctgatacttt agtaattata ggtggagacg
    20701 gcacgattca tcatatcgtc caagcgttcc aaaaaacatt atctcaatac catgtcggat
    20761 tacttccagg tggaactgtc aacaaccttg cacgtgttct tgagattccc ctcaaaaaag
    20821 aagaagctgc tgacgtgatc ctggaaggca atgtacggaa aatagactat ggtcaagtca
    20881 atgatgaagt cattatctct acactgacaa tcggaatctt agcagataca gcagtttgga
    20941 tcagtcaaga agaaaaacag cgttacggct cgtggatctt tatcaagaag ttttttaagt
    21001 tattgctgaa gaagaaaaaa tatcatttgg atttagaaac agacgaggag cattggaaag
    21061 gaaaatccca actgttgaca gtcacgatgt ctaactccgt gggcggtttt acaaattttg
    21121 acgactcagc gaccctagat gatggaaaat ttcatgtaac gatcgtacct tccttagata
    21181 ttttccgatt tattttttat ttgcctagaa tcatgagagg caaattctat acgatccccg
    21241 gcattactta tttcacagcc aaacgcatga aaatccaagc gcaggaaaaa tctgtgggca
    21301 ctcgaacgga tggagacacg acagatgatc ttccagttga attatctgtt gtatcaaaag
    21361 gattaacagt ttattcacca aagaaagaag aaaaagctta gaaatagtag ttaacagaaa
    21421 gttggaagaa gagtaatgga tgtattgtta cacaatcctt tgtggtttta ccttttcatt
    21481 gtgaattttt acttattttg tctgatgggg tatgataaac atcaggctag aactagcggc
    21541 tggcgtgttc ctgagaataa tttgatattt gtcagcctgt taggcggtgg tttgggagga
    21601 ttgatctctc gaaaagtttt ccatcataaa actcggaaaa agaagtttac tataggattc
    21661 gctgcaggtg tacttgttga tttgattctc atttattttt tccattaagg agcaggagat
    21721 gtcgtctaaa ggaacgaaaa ctaaaatcat tctgaatctg cttttgctga ttgtgatttt
    21781 tgccattatt tattatttaa tcaatcaatc atttgcagat atctttgccg aattgctgtc
    21841 taccagtgtt gttgttttgg cagcggctgt tatattaggg acagtttacc aaatcgctga
    21901 agggcgctca atcaaagaaa ttgctgaacc tttcgcgccg aattttacaa cgaaagatgg
    21961 gttcttgacc tctagctata ttgcgtttta ccgaatcgtt tcgtttggta caggaacgct
    22021 gatctcggag atttattttt ataacaaaaa aggaatgaag ctatcccagg gagcaggggt
    22081 gacagcactc catatgatta tgtacaaact ggcagtgatc acttatgcat tagctggctt
    22141 ggtcatccaa ttctctcttt tttattctaa tggtccatca atgatctggt ttatcatcgc
    22201 tggtatcctt ttgacgatcg tgattgttgc ctttttattg gcgatgtccg tcagcttgaa
    22261 tgttcaaata ttttttgtca gcttatcgaa taagttattt aaaagtgagc gtttgcggaa
    22321 atgggtggat acatgcaatc agcagatcta ctctttaagg gaaacggtcc aaaccattat
    22381 ccaagacagg actgcgctat tgcggattta tctatggaat atgttaaaac ttgtattttg
    22441 gtatgtactt ccttatttga ttctgattga aaaccatcct aaaatagatt ttctactgac
    22501 catttcgttt atcagctttg cagtagtgct ttcaggggtt attcctacgc ctgctggtat
    22561 tggttccttt gaatttgtct atttgttatt attcagacca gttgttggaa cagttgacgc
    22621 agtttcttcg cttttactgt atcgtttcag ttcctttatc ttgccatttc tctatggatt
    22681 tgtgtatgtg attgctgaac ggcgaagagt gatcaaagaa gaaataatag aagtaaaaaa
    22741 agagaataaa gaaaatcaag ggtaggtctt ttataagatc tacccttttt atcaaatcaa
    22801 agaaaaacgt tatggataag ataattttac gtttgtttgg ctcttttttt atatacatac
    22861 aatcaatttt gtttttttaa gatttatata agtcgagaaa aaaagtcaaa aaactatgtg
    22921 tatattttga ccaaaattga ccaaagcgat ataatagttt ttgtaaagat caaagaaata
    22981 agtgatctta cctttaatta ggagggtata cctgtatgaa tattgagaaa atgactacga
    23041 cgcttcaaga agcgattgct gaagctcaac agattgcggt cactcgaaaa catcaagata
    23101 tcgacattgc ccatgtatgg aaaatctttt tacaatctaa tcattttggt cgaaatttct
    23161 atacggatgc cggattggat gtcgaatctt ttgaacatga aattgatcgt ctactggatg
    23221 agtatccagt tgtttctggt ggaaacgtcc aatatggaca aaacctcagc cagaatttct
    23281 ttcgattatt gaatgaagca gaccaaatac gtgaaagctt cggcgacgag tttctttcaa
    23341 cggaagtcgt tattttagcg ttgatgaaat taaaaaatta tccgttaact gtttatttga
    23401 ataaaaacgg attatctgaa aaagaattgc gtaaaaacat cgaagagatg agaggaggag
    23461 aaagagtgac ctcacaaaac caagaagaac aatacaaagc attagaaaaa tacggcgttg
    23521 atttagtaca gcaagtcaaa agcggaaaaa tggatccaat catcggtcgt gatgaagaaa
    23581 tccgtgatgt catccgtatt ctttcacgaa aaacaaaaaa taatcctgtt ctgataggag
    23641 aacccggtgt agggaaaacg gcaatcgttg aagggcttgc tcaacgtatc gtacgcaaag
    23701 atgtgcctga aaatttaaaa gataaaacga ttttttcatt agatatgggt gcattgatcg
    23761 ctggagcaaa attccgtggt gaatttgaag aaagactaaa agcagtcttg aaagaagtca
    23821 aaaaaagtga tggacgtatc ctcttgttca tcgatgaaat ccacaatatc gtaggggcag
    23881 gaaaaacaga aggaagtatg gatgccggaa atctgttgaa accaatgctt gctcgcggtg
    23941 aattacattt gatcggtgcc acaactttag atgagtaccg tcaatatatg gaaaaagaca
    24001 aagcgttgga acgtcgcttt caaaaggtat tagtcaaaga accaacagtg gaagatacga
    24061 tcagtattct gcgtggatta aaagaacgtt ttgagatcca ccatggtgtt aacatccatg
    24121 acaacgcatt agtagcagca gctactttat ctgaccgtta tattactgat cggttcttac
    24181 cggacaaagc aatcgatttg atcgatgaag ccagtgcaac gattcgggta gaaatgaact
    24241 ctatgccaac ggaattagat caagttacta gacgtttgat gcagttggaa atcgaagaag
    24301 ctgcactgaa aaaagaatca gatgatgcaa gtaaaaaacg cttgaaaaat ttgcaagaag
    24361 aattagctga acttcgtgaa gaagcaaatg cgatgaagat gcagtgggaa acagaaaaag
    24421 aagaagtcaa ttctgtttca gcaaaacgtg ctgaaatcga taaagcaaaa cacgaattag
    24481 aagatgcaga aaataactat gatttagaac gcgctgctgt attgcgtcat ggtacgatac
    24541 ctcaattaga aaaagaattg aaggaattag aagctaaagc aaaagacagc gagattaaga
    24601 tggttcaaga gtccgttacc gaaaatgaga ttgctcaagt cgttggcaga ttaacgggta
    24661 tccccgtaac gaaacttgta gaaggtgaac gagaaaaact aattaaattg aatgaaacac
    24721 ttcataaacg agtgattggt caagatgaag cagttgatgc ggtcagtgac gcggtaattc
    24781 gttcacgggc tggattgcaa gacccaaatc gtcctctagg ttctttcctt ttcctaggac
    24841 caactggtgt cggaaaaacc gaattagcga aagcgttagc tgagaacttg tttgactcag
    24901 aagatcatat ggtacggatc gatatgagtg aatatatgga aaaacatgct gtgtctcgtc
    24961 tagtcggagc tcctccaggc tatgtaggtt acgaagaagg tggtcaatta acggaagctg
    25021 tcagacgaaa tccttataca atcgtcttgt tggatgaaat cgaaaaagcg catcccgatg
    25081 tcttcaatat cttgttacaa gtcctagatg acggacgctt gactgattca aaagggcgag
    25141 tagtagattt caaaaatacg gttctgatca tgaccagcaa tatcggttca cagttactgc
    25201 tggaaggcgt gactgctgat ggaacgattc cagaagcagt ggctgaacaa gtcaacacgc
    25261 tattacgtgg aaacttcaaa ccagaattct tgaaccgaat cgacgacacg attttattta
    25321 caccattgag cctagacaac gtgaaaggaa tcgttgacaa aatggtggcg caattggcac
    25381 aacgtctgga acatcaagaa atcctgctga caatcagtga tgaagccaaa acgtggattg
    25441 ctgaaaatgc ttatgaacca gcatatggtg caagaccatt gaaacgattt atcactaagg
    25501 aagtagaaac accactagct aaagaaatcg ttgcaggaca tgttatgcct aaatcaaaag
    25561 taacgattac tttacttgat ggacaacttc atttcaaaac agaagagcta gaagaaatcg
    25621 tctaatcttc cattgagact ataaaagaaa aaaggggtct gacacaacta gtgtcagacc
    25681 tccttttttc aaataaacag tgttccagaa gtagtttctt cgaaaaataa gccgaaattt
    25741 cttgaagctg cccgcttctg tcacaacctc ttttattagg tatttttcac tttttctaat
    25801 ttataacttt caaagtaacg ctcatctttt ttactttgag accatgcaaa caatcccatt
    25861 aaggcagcac ctatcgtatt tgtcagcaag tcgcccatcg tatccatcaa tgctgctcgt
    25921 ccgacaaaca gtgtgccatc agaagcagca aaacgttgaa gattcatgcc taagaactgg
    25981 tcgcataaga attcccagaa ctcccaaaac acgccgcaaa gtccagcaaa cgcaaagccg
    26041 aataagagga acaaccaagg actggttttt gatatctcag catctttcat caagtaacca
    26101 atcaatccat agccaagagc tgtcaggacc attggactca ccgcatgtaa gattttgtcc
    26161 caaaaagaga tgatactgat caaatgcatc cctgtgccta aaaagacaga gatgaacaag
    26221 aaaaaccagt aaaacaaaac gattgctgct ggtattttaa gtttgaaaac tttcaaaatg
    26281 acttgtggca tgaaaatcaa tgcaatccct aataaagatt ccacgataaa tgtgattcct
    26341 tgagaagtcg aatatttttg tgtgataaat tcataaaaat tatagcagac aacgaataca
    26401 ccaaaagcaa gtaaagctat ttgcaccttt ttgatttctt taatcactga aagtccctcc
    26461 atctaaaaat ctttttactt ctgaaagcaa ctcaatttct gaagaaacca cttttccgtt
    26521 caatttgatc aaacctattg tatataagtt caggtagtga aattgatttt cagctgtttc
    26581 ttgtagtgca gcgatttttt tcgggtgggt tccgccttgc tgtcgagtat cagtatataa
    26641 gccgatcact gggatgcctt tcgcataagc cacaccgatt tccgatgcta ccccggcatc
    26701 gatcgtcagc ccatctagta aagcaatcat taaatcacta gccaacactt tttctgtgtc
    26761 agcaagagca atcattcgac tgtctgcata agcagttttg tcattgattg cttcattttc
    26821 ttgaggaaga tagatcgaaa gctcagaaga gagctctctg atttctttaa ctaactgttg
    26881 gttatataac aaatcacttt tagcaaacat tggtgcggca aaataaattt tcatctaaac
    26941 atcatccttt ttatcaaaaa taatcgctaa agtaagcttc catgtttgga atgatacgct
    27001 ctagtgactt cagtgtttct tcatctgttt ctaaccgttc gattcttgcc aaataagaag
    27061 cacggcgata attcatcatc aagcaagtca atgtttggat atccagtcga acggattttc
    27121 ctataggttc atttaagaca cggacttgtc cctgttcatc ccatgttaaa gcgaagacac
    27181 cattattcca aggagcgaca ggatcttcaa tgatgaaatg aaaagcatca gctgttccaa
    27241 caaacggaaa ccgttgaagg aattctttta catcaacgat ccgagccata aaatatggtt
    27301 cgatctgctc tttgatctga ctatcttcaa tcaaaaatgc taaaggctca tttttataaa
    27361 tatctccatg tacccagtag atcatggaaa aatgtgcgga aataaagttc cataagccat
    27421 ttcgagcttc ttgatcaaga tagaacattt cttttacgtg aaatatttct tcagctaccc
    27481 agtagaacag aacacctctt ggttcatggt tgctatcgta gtagacagcg gctgtgcgct
    27541 catcttcatt ttcgaaacgc caatactctt cccaatgaaa atcactgcgg aataaagcac
    27601 catggttttg ttgggcaaat ttgtcataaa cggaaaatac gtctggatga tcaactggta
    27661 atcgctcaac cataccactt actgggattt gtttaggtag ctgtgtatct ctgattttaa
    27721 aggaaagttt atcagacatg atttcccatc ctttacgccg atagtagggg atactgtaag
    27781 gatacaaata agagatccat tgcttattct tgcgcatatt atccaatgca acgataatta
    27841 gatcttgcat caatccatgg ttagcatatt ctggataagt tccaactcca gtcacaccac
    27901 ccattttata acgtgcaccg tgaatattga cttcacaagg atagatagcg atttgagaga
    27961 tcagtttatt ttcgtggaac catccgaaca ctttcgatac ttccaaaatc ggttttttgg
    28021 aacggataaa agctcgttta ttctcaaacc cgctttcctc gatgtctgct tcagtcactt
    28081 ggaacacata ggaaagcaat tcattgaatt gatcgatatg ttcttgagtg accggtttta
    28141 attcaagatt ttctctaaaa tctttttgat ccattttttc atctccattt attagcttga
    28201 taacagtata gcaaaattct acaaaaaaaa cttctatagc tgactaatta aaaactcttt
    28261 aaattactgc ttttcgttgg tataatggaa agtgctgatt atatgaaaag aggagacgct
    28321 atggatataa agaaaatgaa agaacgccaa gagcagattc gtaatttttc gatcatcgct
    28381 catatcgacc atgggaaatc tacattggcc gatcgtattt tagaaatgac gcacactgtg
    28441 acgtctcgcg aaatgcaaga tcagctgtta gactcgatgg atttggaacg tgaacgtgga
    28501 atcacgatta aattaaatgc tgtagaactc aattacacag cgaaaaatgg agaaacatat
    28561 atctttcact tgattgacac cccagggcac gtcgacttca cctatgaagt ctctcgaagc
    28621 cttgctgcct gtgagggtgc cgttttagtg gtcgatgcag cacaaggaat cgaagcacaa
    28681 acattggcca atgtttattt agcgttagac aatgatttgg agatcctgcc agtcatcaat
    28741 aaaatcgacc tacccgcagc agatcccgaa cgtgtccgtc aagaaatcga agacgtaatc
    28801 ggaatcgatg ctagtgaagc tgtattagcc agtgccaaat cagggattgg gattgaagat
    28861 attctcgagc agatcgtgga atatgttcct gcaccaagtg gagacttaga tgcaccgcta
    28921 aaagctttga tctttgactc tatttatgat agttaccgcg gtgttgttct aaatgtccgg
    28981 atcacggacg gtatggtcaa acccggagac aaaatcaaac tgatgagtaa tggcaaaaca
    29041 tttgatgtta cagaggtcgg ggtcttctcg ccaaaagcag ttgctagaga ctttttgatg
    29101 gtcggtgatg tcggttatat cacagcaagt atcaaaactg tccaagatac acgagtaggg
    29161 gatacagtaa cgttagcaga caatcctgcc gctgaagctt taccaggtta ccgaaaaatg
    29221 aatccgatgg tttattgtgg tctttatcca attgatactt ctcgttacaa cgatttgcgg
    29281 gaagctttag aaaaactgca attgaatgat gctgctttac aattcgaacc agaaacctct
    29341 caagcattag gttttgggtt ccgctgtgga ttcctcggac ttcttcatat ggatgtcgtc
    29401 caagaacgct tggaacggga attcaatcta gaattgatca cgactgcacc atccgttatt
    29461 taccatgtta ataaaacgga tggtacgact gctacagtag ataacccggc agattttcct
    29521 gaacctgtaa cgattcagga tgtagaagaa ccattcgtca aagcacagat catggtccca
    29581 aatgattttg tcggtgcagt aatggaactt tctcaacgaa aacggggaga attcattacg
    29641 atggattatt tagatgatta ccgagtaaat gtcgtctata atattccgct ttctgaaatt
    29701 gtctttgact ttttcgacaa attgaaatca agcacgaaag gctatgcctc attggattat
    29761 gaaatgtctg gttatcaaaa aagcaagtta gtgaagatgg acatcttgct gaatggagaa
    29821 aaagtcgatg cattaagctt tatcgttcac cgagactttg cctatgaacg tggaaaagca
    29881 atcgttgaaa aactgaaaaa actaatccca cgccaacaat ttgaggtgcc aatccaagca
    29941 gctattggac aaaaaatcgt tgcccgttca gacatcaaag ccttgcgtaa aaacgtcttg
    30001 gctaaatgtt atggtggaga tgtttctcgt aaacgtaaac tcttagagaa acaaaaagaa
    30061 gggaagaagc ggatgaaaca aatcggctcc gtcgaagttc cgcaagaagc cttcatggcc
    30121 gttctgaaaa tggatgagca agatagtgtg aaataactta taatcaatcg aaaaacctat
    30181 taaataaacc tttttgagta gatgcaacac tgaggattat ctgtataaaa gattttaaac
    30241 tgtcatttgg ttatcacggc gatactaaaa ttaacaaaat tggaatattt ttcttgaaga
    30301 tttttagctg tcagtatatg atgaaagcat ctcataggag gtgctttttt catggttgat
    30361 aattcaagtg gaagggttct taaatctcta agaaaagaga aaaaactatc tcaaaaaaag
    30421 ttagctgatt tagcaggtat ttcacaaagt actttagtta aatatgaaaa agggtcaaga
    30481 aaaatcccta aagacgtaga taatactcta agcaagattc tgaatataga aactctaata
    30541 aaagatgagg aagataagat tgagattctc atcggtaaac taattgcata cagagatatg
    30601 aataagttac ttaacaaaga gttagctgat aatataggaa ttagtgaagt attactcagt
    30661 tatgtgttaa atagaaagag aaatccgtca aaagaaatgc aaaaaaaaat cgatattttt
    30721 ctattaagta atgaaaaaga aattttgaag gagattaatc gtgatagtga gatatttagt
    30781 ttatcaaaag acgataaaat tgttatggga aaaagaatac gagaagtccg aaagaaccgg
    30841 gaagagacgc ttgaaaaatt tggtaaaaac tttaccatct atacaggcaa aaatgttata
    30901 agtagatggg aaaaaggtat taatattcca gatattgaaa aactaatgaa tattgcctat
    30961 cttggtaagg tcactgttcc ttaccttatg tacggagaag attataagaa tatacttcca
    31021 aaagatgaaa gagttagcga ttttaaaaag ataaattctt ttagtatggg attaaggatg
    31081 aggaaaatta ggaaagatta ttatctagaa agggaggagt ttggaaagct tttttctcct
    31141 tcaatttcaa aatggtccat agataggtat gagaatggta gagatatacc caatactaat
    31201 cgaattatcc aatatgctta tataggaaat ctttctttag aatttttgat ttacgggata
    31261 taaaaatagt ttacgtcgat acttattaag ttatcgacgt tttttattat agaaaaaatt
    31321 tataaaagga ggtttcttga tatgataaga aacaaaaagg gtttgttgaa agttgcttta
    31381 ctggcagctt ttttctaagt aactttttga caagctcaat ggcaacacaa gcggaagaag
    31441 taatacaaga actaactgtt atgaataaca gctagataaa atgggctatg tatgttatcc
    31501 aacaatttag taatcactag aagcctagcg aaagttcgct tgataagtta tagatgagta
    31561 ttgatttgta gtgtttccaa atcaaaaggg tacagagcca catgaaatat gttgtcctct
    31621 gcagcgtcag gatgggaatc cataccatgg agattcggag atctgtgagg gaatacttac
    31681 gtggttaata tgtcagttgt gttggacgta gtaatggtca tagtgggtag attgactgaa
    31741 ttcttatatg agaaaatgaa accaacaaga agatagggtt atttatacag aattggggat
    31801 cttttctgga aaagtaatcg gataatgcga gattcgtacg gtctttttta aatttctcta
    31861 aaaaaaacgg catcgaatgg gttgggtgat tgataagcaa tgaatcatag actgatatag
    31921 aataaaaaat gattctattt atctttttac atctatcgtt ttttcaagta gccgcaaatt
    31981 atatcagcat tcaacaattt ttgtatacaa gttctagtaa aaggatctga agaaaaagtt
    32041 ggttgttgtg aagataaaaa aagaatcagg ttttactgag tttttcttca aaatttgtgt
    32101 tattaggtta ggacaaagat aagaagaagt atgaagattt aaaaaaggaa tgaggaaaag
    32161 atagtaattt ccgattattg gagttttttt atttataaag aagaagcgaa taagttgtga
    32221 ttcctagatt tcgcgcgtga ttaattaatg agcaaaaaat gcggaaagtt ttagtgttta
    32281 atacacgagg acaaagtttc ttaggtattg aaggcacacg tgtaacggtt gatgggtggt
    32341 gtgattgttt cgaggttgga aaacatgttg aacttatggc atttactgag ctaaaagata
    32401 agaacaacgt ggaatttttc gagagtgata ttggttggga cgatcatgaa gaagtttatg
    32461 gaaaatttat tttgcggaag ggaaattctt atacgaatgg ggaagcataa gtagcgattt
    32521 gttagagatc attgatagga ttgaaattgt cgggaatatt tatgagaatc tagagttatt
    32581 tgaggtgata gaatgagtga tggttttgaa caagtatcaa taattaaaaa tcaagttcgg
    32641 gagattctta gaaaaaaagg ctatttggta gatagttatt ttgaaggaga ttacgaaaca
    32701 tgggtaggtg tctatgctcg tccagagaat aaacctacct atttagatcc tacgacgtca
    32761 gaagatgcct atttacaaaa taggtatcga gtagatggtt ttaagcaaga ctttgcggag
    32821 tggtttgaat gggaaattga aaacggagaa gtaaaagaag aataagagta cttgttgtat
    32881 caaaataagc cagtagaaca accaaaccag tagctagtaa tgacaataat actgaggaat
    32941 cggatcatta tggtatgatt gatatttctc ttccagaaga tcttaagcca gatcctgtaa
    33001 caattggcaa tagtgtcatg atttttgatt cattagatga agctatgtca tatggttgga
    33061 aagaaattaa ggatgaaggt agtaattggt atggcatgag atttggtgat ggtgaagttg
    33121 ccaaaaaagc tggtattcca attgggaaat ggacagttga tttccacgaa gcaggttctt
    33181 tttttgttct gtaattatag tgggagggta aaatcattga tgaaatgtgt tcagataaga
    33241 gcgccagata aaaatagaag agacagttca cgtcgttatc acttttggta acggcgtttt
    33301 gttttattat ataaaattct caggaaaagg aggaattctt gtgtatgcaa aaacgaaaat
    33361 gaattggttg aaagcactcg cattggtggc tttttctcaa ttctgtttct tggtgggaac
    33421 tgctcaagca catgcagaag aaacgacggc tatacaacct acgatggaag ccattgccca
    33481 agtcgcttct gaagtgacag aagtccaacc agcagaagcc ggggtcccag ctggtgaagc
    33541 cactgttgaa caggcccctg tgacagaagc gccaacggtc aattccaaaa cgactctcga
    33601 tcagacgatt ggggaaacac aacacccagg gaatagtcct ggtacttcac aaccagttga
    33661 agaaactgcc tcaattacaa cccctgcttc agaaacgtcc actctagaag gagaaaaagc
    33721 tgccgaagca acgccttctg tgcagccaga gtcttctgag aaacaagcgg aagaaaagaa
    33781 acgaatagaa gaaacagtgg aacaaccagc ggctactgct gacaaagaag gaaaaaatga
    33841 ggaaaacgtt gaaaaaacaa tctcttcaac cgcagctagt caaaatcaac caacgaaacc
    33901 aaacaaagaa tctacggcta gtattgaaaa tcaaaagaaa aaagagcaga acattccaaa
    33961 aacaggtaca cctacactgt tagtaacagt accaaccaat cgttcggttc gtgttcgccg
    34021 tgcaaccaat gcagctgttt taggtggtcg gaaaattgat tggggcgaaa atattggtgt
    34081 tacttctagt ccgaacaatc taaacttcac tttaaattat atggttcgtt ttgagattaa
    34141 tggacaagtg gcgttttgta ttgagcctgg tgtctcagcc gcagaaggtc aagtttatac
    34201 agctactgct ttgaaaaact atttgaaaaa ttcaaatgta cggaaaaaaa caagcttgat
    34261 ctcttattta ggatatgtca caaataaaga caaatccgat gaacaataca ttgcggcaca
    34321 atttatgatt tgggaagctt tgggcacaaa aatcccaatc aaaaatatcc gtgttgatta
    34381 tgatcgtcga aaagcagaaa ttcaacgttt gattaataac tttggtgaaa aagcaacttt
    34441 taataatcaa acacatacgg taaaaattgg cgaaacgttg aaagtaaaga ataaaaatga
    34501 ttttttgaaa tatgtgaaag acattcaaaa accaaaaggt gtcaatgtat ctattcacgg
    34561 caatgaagta tggattacag tcacaaaaga ggcaccggat aaagcaaaaa ttcaattgaa
    34621 taaaattaaa gttcaaggtg cgccattagc ctatcgtcgt ccaggagctc aaacaattgg
    34681 tgtgttacat ccaacagaac ctaatgcgtc tgttattaat ttacaaattc taaaacaagg
    34741 aaacattcgt gtgtttaaag aagatgcgga aactggtgtg aaagcacaag gtgcagcttc
    34801 tttagaaaat gcggtctatg gtttgtatca agcagatggg aaaaagatta aagaaattac
    34861 cttgaaaaaa attaacggca aagtacaggc agaaattaaa gatcttgatc ctggcaaata
    34921 tgttttaaaa gaaatcaaag cgccaaaagg ttatgtgctt tctgatgaag caattgaaat
    34981 tgttgttgaa cctggaaaaa ctgtcaatgc gaccgcaaaa gatcaagtga tcaaaggaac
    35041 cattgattta gtgaaagttg ctaataaaga tttagtcgat tcaacgaatc cagataacaa
    35101 accaaaatta gctggcattg aaatcacttt gacgtcaaaa acaactggca aagtggtgaa
    35161 aaccgttgtg acagacaatg atggttatgc tacttttggt aaaaataccg ttgtctttga
    35221 tacgtacatt ctttctgaaa ccaaaggaaa agaagggtat aaactatttg aaccacttga
    35281 agtcacaatt tctgaaaatg ggcaaacttt ccattatgtt ttagaagata aagtaattga
    35341 acaacgtctt aaagtggtga aagtggatca agaaacagga aaagtgattc cacttgctaa
    35401 tacagaattc aagatttttg atactttagc caatcgttat gtcacaatgg agattccaaa
    35461 tgatacagaa acaacggata tctttaaaac caatgaaaaa ggctttttcc taactaacgg
    35521 tacacttact tatggaaaag atcgctatcg tttggaagaa atcaaagccc ccgtaaatta
    35581 cgttttgaat caaaacccat tagtgttctc gattaccaat gatacagaac cgatcaaagt
    35641 gatccatttt gcgaaccgtc aagcacggaa aagtgtaaaa ttattcaagt atgaagaatg
    35701 gaacaagaag aaaacgccgc tcgcaggtgt gaactttacc ttgtataacg gtaaaaatca
    35761 agcaattggt gaatatacca ctgatcaaaa cggggaaatt ttagtgaaag gtctagttgc
    35821 cgatgactat tacttcttgg aaaatgcccc attagaaagt ttccaaccaa atacagataa
    35881 acaagcattc acggttgcat tcaaacaaaa tgcgccagat gatggggaaa cgttagtggt
    35941 ttctgtacgt aattacttga ttccaccaaa actagaaaca caagcgtcaa ataaagccga
    36001 tggtgaaaaa gtggttgatc cattagaaac gatcgtcatt aaagacagtg tttcttatac
    36061 caacttgttt gtcggaaaag aatacaccat ttcaggagta ttgatgaaca aggcaacgaa
    36121 tgcgccaatt ttacaagatg gcaaagaaat cacaagtacc ttggccttta ttgcagacaa
    36181 agtcaatggc acaaaagacc ttgaatttgt ggttaatgct agtgttctga aaggtcaaac
    36241 gatcgttgtt tttgaaaaac ttttccgtga tggcattgaa gttgcagcac atgcggatat
    36301 caacgatgaa gcccaaagtg tgaaagtgaa taatccaacc gttcatacca cggctgtctt
    36361 tgaagacgga ttgacagtcg cagacccaac aggattgttt accttaaacg ataccgttga
    36421 aattaccgag attcttccag gaaaagcata cttggtcaaa gggatcttaa tggataaagc
    36481 aaccggtgaa cccttgcttg tgcatggaaa tacggtagaa acgagtgttg gctttattgc
    36541 caaagcagaa aatgaaacgg tgatcgtacc atttaccttt gatttgacag gtataaatgg
    36601 tcgtgaaatc gtcgtgtttg aaagtctttt tagaaatgat gaagaactag caaaccataa
    36661 agacatcaac gacaaagggc aaacggtacg agtaactaac ccaagtgtac gtacaacggc
    36721 tacacataaa cccactggct tgaaagaggt taatccatta acgaaagtca ccattacaga
    36781 cgctgtgaaa taccacgatt tggtcgttgg aaaaacatac acagtcaacg gtgttctaat
    36841 ggacaaagta acggaaaaac cagtgctagt agatggaaaa gaagtcacaa gtaccttaac
    36901 gtttgtggca gaaacaacgg atggtacagt caatctagac tttattctag acgcacgtgc
    36961 gttgcgaggc aaagaagtcg tggtctttga agatctgttt agagaaggcg tattattggc
    37021 ttctcacgca gatattaccg acaatgacca aaccatcaaa atcagtaatc caaccattac
    37081 gacgaaagcg acgaataaaa atggcggaaa agagatcttg gccttgccaa atcaaactgt
    37141 gcttgaatgg gtcaaagtag acggtcttgc gattggtcaa atctatcgct taattgttca
    37201 aggctactta acaccagacg gcacaccatt tgaaggcact catgcggaaa aagtctttgc
    37261 ggccgataaa gaaatgatgg aattcttatt tgaattctta gtggacggtc gcaatcttgc
    37321 aggaaaaggc ttgagttttg cggaatggct ccaagcgaaa acggaagatg agaaagaaac
    37381 gtttgaagaa gtggcgaaac acaatgtaga tctaacaaac aaagatcaaa cggttcaaat
    37441 tgtggaagcg ccaaaaccac cgcaaccagt gaaaccagaa caacgggtta aaccagctac
    37501 tgttgtacca atgttgccaa aaacaggtga acaaacaagt agtatactta tggttcttgg
    37561 attagtagta ttagtcattg ctttaggtat aacactttat aaagtacgta aagccaaaga
    37621 ttagtcatga ggaacaagcg gttaggattt ttaaccgctt gtttgattgt aatgtagaaa
    37681 tatttagaat ttattttggt ttttaagagg aggaaaaact ttatggcaga aaaagcattg
    37741 cgtatgaaga aagagttttt aggtcaagta tttttattgg gttcacggga acctaatcca
    37801 ccaaaggaaa ttaatggtgt gtctgggcga tatgttcaat tacaagcaga gtgttcgcga
    37861 gaagaacagc ctgctttttt tgtttatctc acagctgaag aggataaaag attacgatta
    37921 ccagaaaaag aggtgcgtat ttcatttgaa gaagcttatt ttgtttttcg taaagaacga
    37981 gtgaatagtc gtgaaacaaa tgatatggga caaacagagc gtcgaacaac tggctatcaa
    38041 gatgttggtg tctttttaac tggtgtaaaa attggggagg ttttgtaaga tgggattaaa
    38101 atttccaaat aattatgaca ttacagaagc attagatgaa acttgtttag gagccgtacg
    38161 ctttggcgca ttagaagagt ctgatcgtgt atttgataat gaaacaggcg attatacggg
    38221 agaaattcga tcacagcgag cacaattagt ttttggaaat ccagatgaac caaagattgc
    38281 acgacgagtt aaatttccag cagatgtaga tttgactggt tttaaacgac gtgatcctgt
    38341 gaaattaatt gatccagaat atcatgtggt atatattcca gcacaagatg gcaatcgtga
    38401 atattcagat ttagtgattt atgcgaaagg attagagaaa gtagaagctc caacaaataa
    38461 tcctaatatg aaacaatcac aagaaaaagg tttagatcaa aaacaaccaa gtgttgattc
    38521 taaggaaaaa gagaaaaaat aaggtttttt tgaaagaata ggaaggagtc ttcctattct
    38581 ttttatctaa gatagaagga aggggagatt ttgaattcag ataaaaccaa tcaaatactt
    38641 tgagagattg tgcttgttat caaaatatcc aacaattgat tgagtatttt ggagacaatc
    38701 caacgatttt tgtacaaaca aatgcatata tttgtgcggt tattgggcag tgtatgtttt
    38761 tacgtgatct tgttcgtttt tcattaccgt accagtggaa acaagatcat caggaagtga
    38821 tttggattcg tatagtacga atatgtaata tgtgtgcaca cccttataaa aatcgattaa
    38881 atctttttcg attatgtttt tttactaaat gtgatattcc tgtattagtt gctttttgtc
    38941 aaaagttttt agcacaggaa aagtaaaaac agaaaggagg aatgtgacaa ttgaaagctc
    39001 attttttcaa gtatcgtggt cggcgaattc gctatttcaa tcgttattta tggttttgct
    39061 attatggttt ctttttgttt ccatttttga ttctgttagg ttggtttagt tggaaagtat
    39121 tttatccacg tttaagtacg ttttcgaatt ggcaaatgta tcttattcct ggaataagta
    39181 ttgtgttgta tttgttgctg gttagtgggt taattttagg gttgatgcat tggtcgcgac
    39241 taaaagaagg atattttgcc tctgtttatt ggcgtcagtt acttgttcga atgttaattg
    39301 acaatcggtt tgtttataca aagaaacaag ttagtgagaa acaaacaagg gaaaaaatga
    39361 gattaccaaa agtttatttt tatcaaaaga aacaagaatt agtggtttct tttcctttag
    39421 atggtgggcg ttttcacgaa cgatttttaa aaatgggtca tttgttatct gaagtatttt
    39481 tgcgtgattt aattcgtgaa gaacgagaaa aagcccgaat caattatgta ttcctttcag
    39541 attctggtcg gattcctatt gatcaatgta tagcagaaaa tggacgtatt catttaatga
    39601 aaactttgga ttgggtatat gatcaatcac caaatatgtt gattgctggt gcaatcggtg
    39661 gtgggaaaac ctatttattg tattctttaa ttcaagcttg tttaagtgtt gggacagtag
    39721 atatttgcga tggaaaagcg gcagatttag cagcattagg tgacgtcgat gtattcaaag
    39781 gacatgtgtt ttacaaaata aatgaagaaa tgattatttg tttacgaaat gcattaaaag
    39841 aaatgaatca acggtatgcc tatatgaaaa ccatgaaaga tcctaaatat gaaccaggga
    39901 aaaattatgc ttattatggg ttaccaccgc attttatctt ttttgatgaa tgggcagctt
    39961 tttatggttc gttggatatg gaagcgagaa atagagtcga taaattgatt cagccattag
    40021 tattaaaggg tcgtcaagca gggatgtaca ttattttagc aatgcaacga ccagatgcag
    40081 aatattttcc aagtggtgtt cgggataatt taacattccg tatttctgtt ggtcgattgt
    40141 ctcccattgg ttatttaatg acttttggag aagattttaa aaataagcca ttttacaaca
    40201 aagatcaacc tggtcgtgga tatgcttttc caggatttga tgtacctgta gagttttttg
    40261 cgccatttgt ttacaaagat tatcacttta tcaaagaatt tgagaaattt actgaaatga
    40321 ttgtctatga ttttacagta aaagaggata gtcaagatca aacgatggga gatgataatc
    40381 agttgttaca agaacaacta gagcgccaaa tgtatttagc aaaagatcca aaaggaacaa
    40441 gtttttctga tgcgcatatt ttgtaatcaa ctaaggaggt gaagttaaat ggcagagagt
    40501 tgttattata gagtagctac agcaatacga atgattaatc cttctctttc ttccagaacg
    40561 ttttatgatt ggttgaatcg tattgaacaa gtaactgatt accgcttttt gcgcaaagaa
    40621 cgtgttttta caggaaaagt tattaatcaa gtattgctaa cgaaaaaaga tatagaacga
    40681 ttgactcgat tgtatcatta tcgagtagat ttagaagagg atttaacttt gtcaatttat
    40741 cgcgtttttt ctcctgaaaa atatagtgaa ataacaaaat tagatcattt aattttgtag
    40801 ttattatcta tcgggagggc tctagacgta gtctggtagc cgcactctgc ctggcggtgc
    40861 gcattaaggc tgcgaagcat gggcctataa tgcgcactcg ctagcagcgt gtgcagctca
    40921 caaagaggtc gtgcgcttgc gcacggcctc tttgtgtaaa gccccccgat atataatatg
    40981 gggggcagaa ataacataac acatagaaaa aagttttata aacccttttg tatcaagtga
    41041 ttatctaagg tcagcagttg tactactttt cccaaaaaac gagggggact agttgatata
    41101 tcggtgtttc taaagggtta aaaaaaatcg attttgttat tttataaaag gagcgagaaa
    41161 atgacaccga ttgagctaaa aagctacaga aagaagttaa gtgcaattca taatataagt
    41221 ggagataaaa agccgctatc acaagcaaga tttgcgcaat tgatgggctg ctcaaaagat
    41281 tacattagaa agatggaaca agggaaaaga aaaatcccag aggattttga acaacgaatc
    41341 aaggaacgct ttcgattgta tatgaaaagc ggaatagatc ttcgagcaat gattgattat
    41401 ttgcgtcttt ccttttttgg gacagactac aaaacagtga ttcaacgtgt attacgaatg
    41461 gatctgacag aatttgaaac acgtccaacg ggcttgtatc tttttgactg tctttctgtt
    41521 cgtgggaata tctgggtgtt ctggcattca aaacgaactg taacagaaaa tgtacttgtg
    41581 caattttcag gacaaggttg ccgggaatat gaggaagtca tgcgtgaaca acaaacagat
    41641 tggcgaacat ggctatattc catttggaaa aatcaaggaa agttggaagc tggttataca
    41701 cgtgtgcaat gtagccgatt cgatattgcg ttagatgaac tttggggatt ggaaacggaa
    41761 gaacatttcg atctctttga attactagaa aaacaaaaag caggtttatt agaaatggac
    41821 tttaagagat tccagaatat tggcggttgt tttttagatg atggctattt tcgaagtgaa
    41881 ggattatctt tgtattttgg atcgcgaaag agtcctctct tttttaattt ttatgagaaa
    41941 cgttttgaaa ttgcgaaccg agaaaaaatt tcagaattag aagcattaac gaaatatggt
    42001 atttataatc gatatgagtt acggttagcc aatgagaaag caacacaagc agtagaagca
    42061 tttattttag gtgattcacc gaaacgttta ggagagattg gcgttggttt aattaatgca
    42121 aaacttattg tttgggatag actggaaagt gatgcacgga ttgttgataa acgatgggca
    42181 aaattatttc gttcgatgga agaattggat tttacagtaa agccagaaaa attctcatta
    42241 gatcgttcag aacgctggtt tttgaaacaa gtagctccga cattaaagtt gttacaagaa
    42301 gcagatcgta ttttaggaag agactttgta caagaaggga ttgataatgc cgaactaaat
    42361 gacgagcaac aaaactatct agaatggatg ggaacgtcag gagggaaaat tgcatgaatg
    42421 catatacgac aacagaatgg ctgcgcctgt tggatttaaa ggctgcaatc gaagcattaa
    42481 atgaaaagat ggttgatttg tcttattttc gttttcgagt tccttatatt gaacaagcgg
    42541 taaaagctgg acgttatcag gaaaaagaga actggcaaga aattgcacgc ttattagaag
    42601 ttcgtaaggg atacgaacaa gaactcgaag agttggaatt tagccgaagg aaaggaaggc
    42661 tagaattcat tcgcttttat cgattttcat tgccaatacc tgcgatttta gcggttaaaa
    42721 aaggctgcga taaaatgaag atatatgaaa actgtgtggc agcacttagc agcgaaaaac
    42781 ctcttatgga agaaatttca ttggtgactg tcacttggga aatgagtcgc cagccaacag
    42841 aagaacagac atatctttct ttagaagaga ttgagataga attagaggaa attggtcgct
    42901 atgccacttg ttctacctat tgtggatcgg tcattagtat tgctggtgtg attgtctaaa
    42961 tgaaaaaaat tatgatttat gttggtgcgt attggtcacg tgatccaaca gtattagaaa
    43021 atccagaggc ggtacactat tgcttgcgcc aattattcta tctgtataaa gaacgattag
    43081 aatcgttgat tcgtcagtta ccatatactg acagacgttt agatgaactt cttctaaggt
    43141 atccagcaat gtataaacga cgaaaaaata gattgttacc agaagagtat cccattgaaa
    43201 aacatgaact tgaagggcga tttgtggctt atttctatga tgatgttcgt atgcgcttag
    43261 tagaacaacg aatggaaata gaaaatgatc gctactattt tatcaattat tgtaagaagc
    43321 gtaagtatca agtaactgat gatttttatg attgtatttt acaagatggg gaagtgattc
    43381 tatccaaaat tgctccaagt tttcgtgaac ttgtgccgat tgattttttg aaaaaatgtc
    43441 atttgcggat tcttccgtaa aaaagagcta taaaaggaaa gaaaagtaga agccaaatcg
    43501 gtttctgctt ttttattttt tttaggaagg agaagatggt atgtcactgg aatttttagg
    43561 agagccaatt gaaaaggaag agttcgtttc gcattatatg gatttgttta ttcaggtgat
    43621 aaaaaacttt gtgcgattga tgaattttat ccacgcgaac aagactatct agccgctgaa
    43681 cgaataaaaa tcagtttatt atgggaaaaa ttcacagatg aaaataaccg accgccagat
    43741 tatcgctatt tatcagattg tttaacgaat aatgtgcttt ctaaagaata cctatttcac
    43801 aaacgtgaga aaaatcaaat gtcggttgaa catttttgtg attgttatat tgcattattg
    43861 cgtagtcaac agttattaac agtagaacga tatggtgcag agtttggtta tgtcatgcaa
    43921 gctgagcgtc atagagctac aagatgctat gaaaaagaag atcaagtgat tgatggctat
    43981 gaagaattat gtattcgaaa caatcgctat ttgcaacaac gtttatatga acaattgatg
    44041 atggattttt caaatctcta tagagggtat gaatgttcga tgattcaaga aaggtagaag
    44101 aaaaggggga atttttatgt ggcgattatt atttttggtg gtatgtggta tggcattgtt
    44161 ctatgtgtat cgtgtctatc tctatccacg aatggaacga tggacaagtt ggtatagaga
    44221 tcctttaaaa caacgaaaag gattgaagcg gtatcgttat gaattacttg ttgaattttc
    44281 ggatgggcga caagcacagt attatttttt tgcaaactat ccaaaataca acaccagtga
    44341 actattgatt caattattca aaaataatcc atatgttggg ttagaagaaa acggacggga
    44401 gttcttttat cgttcagata atattagtgc ggcgtctatg aacaaaacac gaattgcata
    44461 aaaaattgga ggaataatca tgaaaaaaac aacattagca gtcgtaacaa cactaggaat
    44521 ttcattattt tcgtttcaat tagggagttc attggtttat gcagatgaaa ctgtagaaac
    44581 acctaagtca gaaattgtag aaacattgcc aggggaagga ttaccagatg ttgagacaga
    44641 gctaaatgga ggaacaacgg aaaaaccagc agaacctgaa caattgcagt cagagaaacc
    44701 tgaaaaagag ccagtgacac cagaaccaga gtttcctgaa tcaactttac cgactgattc
    44761 gatcgaagaa gaaggtgtgc atagttcaac aacaacggat tcaagtatag tcacaactcc
    44821 ggaacaatca ggaacaacca gttcttctga aaagaatact gattcgtcaa gtacggaaca
    44881 gccaacgatg tcgtctacca gtgaagaaca aaaagaagaa ccgaaacaac ctgttgaaaa
    44941 atcagaagca attccaaaag aaaataaacc tattgttcca gcaaaagaag tcacagtatc
    45001 ggtaacccca agcggtgaaa ttacgacgaa tacttctcaa ggaatgagtg taccgattgt
    45061 cactagtaat gtagaggaat tgacccatat tccaacacca accaccccat taaaagtagc
    45121 aggtgatcaa acaattgttg gtgtaaaaga tggtattcca ttaatccaag atagtgaagg
    45181 gaatttggta aaggatttgt cgattcctgt aaagaaactt ccaagtggca atattgaaat
    45241 caaaacagca gatggaaaaa caaaagtctt accaaagact ggtgaagaaa tacatgttgc
    45301 actttctgtt ctaggaggag tgttgacaag tattgctgga tttgttggat ataaaagaaa
    45361 gagaaaagga atagaaaatg agggtaattg aatggactat ttttcagaaa aacaagctta
    45421 tcgttgtcaa tcgtacgtac tagtaaaaac atactagaag gagtagctta tgaaaaaacg
    45481 acaactacgg cggaaagaaa aagtttatac agagaaagta ccgaagcaaa agaaaatcca
    45541 ttcaaaaaaa gcagtgaaat ggatttggtg tttggtttta tttctttgtt tttcaggggg
    45601 cttggcattt tttcaatcca tgaaaacgaa ggttcaagtg tcgtttttga aagatgaaat
    45661 ccaacaatta caaaagaaag aagtacaaag tcggtcagaa ttaacgtaca cacctgaaat
    45721 tgaatccttt atgaatcgtt ttgttgcttt gtatatgaat acattcgata atcatgaaca
    45781 agaacaacaa cgtcaagaaa cattaaaaaa agagttttat gcacaaaact ttattcaaga
    45841 accaggaagt gtttcaggtg ttcgaaaatt aactgctgct tcgtttattg gattaaaaga
    45901 aattgaaacc attccaactg ctagttataa agtaacttat gatattcgct cgatgaatcc
    45961 agagagtaag aaagaaacaa gtgtgacaat cacacaagta ctcaatcttc ccttttctat
    46021 ttcaaacaag ggctgtcgta ttattgctta tccttatttt acggcactac caaagaatac
    46081 agaaaaacaa cgaatgatta cttatgatga cacattatac gaaacggttg atgcgtcgga
    46141 agcgaaagat gttgaaggat ttgtggaaga tttcttagat aagtatgcga aaaacaatgc
    46201 aaaagatatg tcatacatga tgaaagaacc tgaaggactc aatcaaacag ctaaagttga
    46261 tcaagtagag atgaaagtat tgcgttataa agaaacgaaa aaacctttta ttgtaaaagc
    46321 aacggtcgtc tttcgagatg gtgatacgtc acttttacaa aaagaacaaa tgacgattgt
    46381 cattacgaaa aaaacaaatc agtattatgt tgaaaaatta acacataatt ggttggatag
    46441 gaaaggagaa gaacggaaat gacactgaag ggaattgtag attttatatt aacgcaaggt
    46501 ggctattttt taataatggt ggttgtttat ctgggattgc gaaattttat gaaatcaaaa
    46561 gtaggaaatg cattaatatc tatcgcagca ggtgccgcag tttatttctt cttaaatgat
    46621 ccaacacgtg tatttgatgc gtttggtaaa atcattgaaa aattatttat ggattaggag
    46681 gatagtgaag tggattggga accttatgat tattcgattg tttatgcgga accttatcgc
    46741 gtacaacaga ttacaagcaa ttggcgtctt ccatttccag ttgcattagt caatgcagca
    46801 acttttttgt tggtcgtaat ctttgaatat ttatttttac gttcatttat tggttgggtc
    46861 atttcttttt ttccaccagc aggagttgtt ttttatatag gactccccta tttatgtgtg
    46921 ttagcactgg caaaactaaa acctgatgga aaaaaagtgc atttttattt atgggattgg
    46981 ttttgttata tgattcgtat gatgaatggt gattgtgtgt attataactg ggacaaggta
    47041 tcaaacaatg cattggaaga attacttttt gtgaataagt atgtttattt agaggattag
    47101 aaaaagggac aatcaactgg ttgttccttt tttattggga ggtgaaattc attggtgctt
    47161 gtaaaagaac cagtaaaagc attcaagaaa aattgtttgt taaatggaga ggattgtatc
    47221 tgggcctact atcatgtttt tccacgaaca atttcaaaaa acacaccaaa aaagacacag
    47281 gaaataaaag aaatgatggc tcgtttactg ttgcttttag cagattatga agatgtggag
    47341 ttggtgatgt atccacgaac aatgaattta cggacgaagt ttgacacatt aggaaaggga
    47401 ttttctcccg atagtcaagc aattggtgcg tattatgcaa aagaaacagt gacattgttg
    47461 gaacgagaag gtcaagtcta tcgtccttca tttattttag gaatcaaagt caaagaaaaa
    47521 gcaacggtgg agagttttca tgatgtaata aatctaatct ggttagattt agcaggaagt
    47581 ttagcacgct tatttggatt agaagtagat gatttggaaa ctttgttttc tgaaagtgat
    47641 ccttatgtaa aagaagtgac acaattactt tatagctttt ctattcgtcc cttagtaaaa
    47701 aaagagctgt tgtatctcaa tcgtctgcaa tatattcgag gaatggctca caaagtagaa
    47761 gaacaaaata attgggaagt actgaaagac aatcaaggaa cgtatgtacg tataccgaag
    47821 gaacataaag gccttcttca attagaagat gagttaggga gtagttattt agcattgttg
    47881 cctattgggg aaacacctga aaatatgaga aattgtgatc tatttcaatt tgctcaggat
    47941 ctttctttcc cagcagaatt gcgaatgaaa attcactatc cacgcttgaa aggacaggga
    48001 ggtattggtt taaaactgaa ttggctatcg gaacggttca aaacagagca taaggaattg
    48061 gttcaaaatg atgaagaacc tagtgaacgc ttattaactg ttcgaggatt agtaaaacat
    48121 ttaaagcaac gagtagataa tgggcaacct gtcgtcgatt ggatcgcatg ttttattgta
    48181 tacggtcaaa cggttgaaga agttcgtcaa aatagtgcaa cggttattca acaacttcat
    48241 acgattatcc atatccaccg tgcaaaaaat gatcaacgag aattgttcta taaatgttta
    48301 ttagggcaac cattgggacg gcagttacat tggaagcacc gaacaactgt agaagcagtt
    48361 ggcgaatggt tgttttcagc agtatcagaa ttaggtatgg aatccggctg gtatattggt
    48421 cggcaagata cattagggga tggtggttct cgaggagaaa aaacaccatt agaagaatta
    48481 atttatgcgt caaatcgttt tgtctttttg aatcttctag ccattgcgga aggaatcaaa
    48541 ggtgcgttgt ttgatgcgcc tcatgtagca gtaactggaa aaaccggtaa agggaagacc
    48601 tttttagttg gcttgctctt tttgtatagt tcgtttatga atgtacaaag tttatttgtg
    48661 gatcccaaag gagaaaagcg caaatggttt acgaaactca ttcaagaccc gtattatcag
    48721 aaacattacc ctttgtttgt agaacatttg caaaaaatga gttatgtcac gttagatgct
    48781 acaaatcccc gtaattatgg tgtattagat ccaattgttt gtttatcccg agtagatgcg
    48841 aaacaagtag cccaggatat gattgatgag ttaagtccgt taggtaccaa tcacctttta
    48901 aaaggggcgg tattaaaggg aatccaagaa gttttagttc aaaaagaagc cggacaacaa
    48961 gttggcttga tgcatgtggt cgactattta gaacaaatgg aacaaaaaga ggttcgagaa
    49021 tatggtgagt ttttacgctt gacggtagaa gattcgattt tacgtttagg attttcttat
    49081 ggagaaaacc cgggattaga ctttaatgcc aaaacaacga ttttagagat ccaagatttg
    49141 aaattaccaa atgacaatgt ccgaccagaa ctatatacag atgcggatcg aaagtctttg
    49201 tgtttgatga ttagtttagg tcgtttttgt gaaatgtttg gaaaacgaga tagtagtaag
    49261 aaaacagcga tttattttac agaagcatgg gtatttaata attcgaacgc aggacgtagt
    49321 attattgcag caatggcacg tgtggggcgt tcccagatga atcaattagt gttagataca
    49381 caatttattg gtgacttagg ttcagataaa gaaaaaggga attttggtgt cgtctttgct
    49441 tttgatgaag acagtgagcg agaaaagatt ttgaatcatt tacgtttgga agtaaatgaa
    49501 gcgaacttag ctgaaatgag ccacatgatt aaaggacagt gttggatgtt agatccctat
    49561 ggacgtgtag gaaaactcaa tgtacacagt ttgtttgaag aaatgaccgc tgcattacaa
    49621 acaacggaaa aaacaagtca ctctcaagta gaagaacagg atacgttaat catgcaatag
    49681 aaatgaggtg aaacaatggc ttatataaag cataaggaaa agaaatggtg cgttttgttt
    49741 tttttaacga ttggattcgg actgcttttt gttggtggat tggtgtatgc ggcagaaaag
    49801 caggtggatc aatcgatcta tgagattggg aaatttcata ttgatacctt ttcttcttat
    49861 ttatctgata gtgaattttc attaggcaaa atgttatttg gggcaaatgg cacagatttt
    49921 ctaaaaatga tgacgaatat gttctttagt ttaagtaaat tgatttggca aggccttgat
    49981 tttgtcattg agaagctcta tgaaggagct gcaatgaacg agttaattca ccagtttttc
    50041 actttttcgg atactattta ttcaaaaata tacgatccta ttggtgcggg gattgtcgtc
    50101 ttgtatgtgt tatatctatt tactcttcgt atatttagag ggaaacatta cgcaaaaatg
    50161 gtgtttattc gatttctctt cgttgtcatt ttttcaatgg tttggtttgg ctatggtagt
    50221 aacgtaccta gtaaaggaga agcttttacc aaagggttaa atgatttgtc cgtggaggta
    50281 gaaggcttta ttttttctgc tacaaatagt ctagatgaat tggcgacaaa agatgtcaat
    50341 ggtgttttag tgccaacgac acaaaaggaa gccattcaaa aagtgcgcca actttattat
    50401 aaagcggctg ttgttgatcc gtatatattg ttaaattatg ggacaacaaa tttagaagaa
    50461 ttgtatcaag cagagattga accacaagaa tttctagcca aggaagcgtc aagtgaagaa
    50521 ttatctgata ttgagacaaa agtgaccact gctgcaggag aagatagagg gaaaaagaag
    50581 gcttatcgtg tctatattca accaactaaa gctgtttata aagcagtcgt tggttctatg
    50641 gtgccattgc ttaatctaag tattggatta ccacttgtag tgattggcat gattcgtttt
    50701 atctttcaaa tgttggtgtt gtgtttatta cttgtgattc ctttttcatt actcgcttct
    50761 tttgttccag tatttgatta tatgctcatg aataccttaa agtccttttt gggcttgatg
    50821 tttcaaaaat ctttttacag tgtgattatt ttggtggcat ttttgatcta taacatagta
    50881 gacgatttaa ttccaatgaa tactgttttt ggttttttag gaaatatgtt tgtcaaagca
    50941 ctcctatcaa ttttactcat tgtaaaacgg aaagatttat taaagaaact agggttagga
    51001 gctgcagatc aaactataaa caacacgaaa aacatagcaa gtcattcttt gtctcaaatg
    51061 aaacaactgc ctggtcaact atcaactgct ggacaaaatt tggcaatcaa aggtgcagga
    51121 ttagcaggga atgtgtatcg agcaacgaat ccactcgctt ctgcagcgat acatggattt
    51181 caaaagtttc aacaaaaacg tacaccacaa gaatcggtaa aagggaggca agtggttaat
    51241 ccctttgaac taggtgggcg ttcaaatcaa gccttgcctt atagtggtcg tcatgttcgt
    51301 tatcatgggg tagagcctga aatgtcttct accaaatcga aaacagagtg gaattctggt
    51361 ctttcaaatg gtttgattcc aaattcgtcc atgaattcag taagaccaaa acaacaggta
    51421 caatcattgg cacaattgag taagcaacaa gggaataatc aaagaaaacc attgttaaat
    51481 aaaggtatcg cttttgttgg tcaatcgatt ccgttgcctt ctggaaaagt acccgtgcca
    51541 caattaacag ctgctggttc tctttcacaa cagattcgcc aagtgaaaaa tcaggcgaaa
    51601 aatgggagtt atccagcaat gcagcagtta ttgaaccaat actctgttcc taattcgtcg
    51661 gtagccactc tatcaggaaa tcgttctgtg caacaaccgg ggcaacgttc gtctgaaagt
    51721 aaaccgtcga tttccgcacc aaaatcgtta caacctgcat tttcttcttt agataagcca
    51781 acagagcaat cggaaaaaat agaaaaggat cctaaaacac tgccgatttt ggacgcaaaa
    51841 ccaggaactg attttacttt accaaaagga cgaacagtcc aaaaaccatt atcgaagaaa
    51901 ggatgaggaa gaaatgaaga aagggtgtct aggttgtctt cttttcttta ttcttccctt
    51961 ttttctattg ccattctttt tttcaggaga actttctgag catggaggag aagctacagg
    52021 tctagctgtc gcttttaatg gggaatatac cgaaggatta ccgatttttt cagagattaa
    52081 aggacgtggt ccaatcacag atgaacatgc gcaagcagca gttggtgcag ctgtaaagta
    52141 tcagttatta ccgagtgtga ttctttctca attaggctat gaatcagctt atggacagtc
    52201 acaatctgga agaaacgata cgaacttttt tgggattacc tggtttcctg gttgtcccta
    52261 cccacaaggt tctgcacgtg gaattggtgg ttctgaaggt ggtttctata tgaagtttcc
    52321 gaacttaaaa gcttgttatg actattatgg ctttatggtg gcgagccaaa gcaactttaa
    52381 tcaatgtgtg ggcaataaag atcctggaag ttgtttattg attctaggtc gtggaggcta
    52441 tgcggcagca ggtattaccg aagggagtcc gtattatcga ggagcaatga gtatcataac
    52501 gtcctataac ttaacggaat atgatacttt tgcgatcaag cattggaata gcctaccaac
    52561 aaaaaaaact tcgcttattg caggaaaaag taaaggcaat gcgaaggttt tggatcggat
    52621 cattggacga tccgtaaata atggggaatg ttatggtggt acggcatggt atgtatctca
    52681 attaggtggc ccacagttaa tgggttctgg acacatgttt gcgatgttaa taggggaaga
    52741 ttataactgg acaacctatg gttggcaagt aatcatgaac ccaaaaccaa gtgatttaca
    52801 agcaggagat gtggtgaatt ggcaagcagg tggtgcattg tctcctggaa tctatggtca
    52861 caccggaatt ataacagatg tttcatatgg cggacaacat ttctctagtg tggaacagaa
    52921 tgccgagcaa ggacggatta tggctcggta tcaacgcacg tacaatcaaa caaaaattcg
    52981 tagtctggtt cgtaaggtga aataagaaag gcagtgacta gaaacgtgaa aaaagggata
    53041 attttagtat ttagttggtt atgtttgata ggtcttactg gttgtcattg gttttcttct
    53101 gaaaaacaaa aaacacaatt gagaacgaca gaagaaacaa tcgaagcagt aaaagaaaca
    53161 agtggaactc ttcaaagtac tgaaagtaac agtcaaattg gttcattaga agacgagaca
    53221 gcgcaacaaa tgcaaataga tgaaatgaca aaaaatttct taaaagattt tgctagaaaa
    53281 tggagtaatt tcacagatgt ttataaacgc aatcaagctg tgagggacta tatgacagat
    53341 cgttgtatta aagacaatag tattgatgta gacccgcatg tacaaatgaa aacaactggg
    53401 aaactttacc aggtgacaca agatatagag aatccggatc aatatttgtt atttggagaa
    53461 gaaacaatga ataaggttac tcaatttgta ttacttcaat tgaaagtaga tcaagaagct
    53521 caaaagattg atgcgatcaa gatttattat gtacggagtg cgtattaaaa taaaaaatga
    53581 acactcatat ttattataca ccgaagaaat agaaaaaaac agactgtttt tttaagaaaa
    53641 actatgtcat tctttacttg aaaaaagttg ttataggaca gaaaggataa aacaaaaacg
    53701 gaaaaaatat gtcttatgaa taactataag aactattcta ttttttagga gatggatacg
    53761 agatgaaaaa actaattggc tatagtgtga tgtgtattgg atttgttggt gtgggattgt
    53821 ttgtatcgac tgaatctgct tctgcggctg aatggtataa tcctatctgg tataatccga
    53881 acattgaatg tcaaaaaggc tatccgtatg gaccgacaaa tctgaaaaat ggaaaatgta
    53941 gagtaaattg gaatggcgtt gtagatagtg taattactgg ttcaatggac tctgctctac
    54001 atggctttac gggtcactag aaggaggaag aaaatgtctg aagagaaaaa agaaatcgtt
    54061 gaatcattag tagctttaaa agattctctt tctaagattg aatatgttga taggcaagaa
    54121 attcaaaaac taattgatga tactattatt gaaatccaag atgcgcgttg tgaaggtata
    54181 aaaatttctg tagctttatc taaagtaata gaaaaaatga accgttcatt agcgtttaat
    54241 ggattgaagc tagatagaca aactagtctg atatgggatc atttaaaaga cttgtatgac
    54301 aagtctaaaa gaagtgaacg ga
//
LOCUS       DACDIE010000009        51951 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.009, whole genome shotgun sequence.
ACCESSION   DACDIE010000009 DACDIE010000000
VERSION     DACDIE010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51951
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.009"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..98)
                     /locus_tag="IV250_000666"
     CDS             complement(<1..98)
                     /locus_tag="IV250_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP8767972.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGI"
     gene            790..948
                     /locus_tag="IV250_000667"
     CDS             790..948
                     /locus_tag="IV250_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8767973.1"
                     /translation="MNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKD
                     FMNTFKIT"
     gene            1020..2246
                     /locus_tag="IV250_000668"
     CDS             1020..2246
                     /locus_tag="IV250_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8767974.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(2334..2726)
                     /gene="rpsI"
                     /locus_tag="IV250_000669"
     CDS             complement(2334..2726)
                     /gene="rpsI"
                     /locus_tag="IV250_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP8767975.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(2742..3185)
                     /gene="rplM"
                     /locus_tag="IV250_000670"
     CDS             complement(2742..3185)
                     /gene="rplM"
                     /locus_tag="IV250_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAP8767976.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(3704..4171)
                     /locus_tag="IV250_000671"
     CDS             complement(3704..4171)
                     /locus_tag="IV250_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAP8767977.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(4291..5829)
                     /locus_tag="IV250_000672"
     CDS             complement(4291..5829)
                     /locus_tag="IV250_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAP8767978.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            5909..6595
                     /locus_tag="IV250_000673"
     CDS             5909..6595
                     /locus_tag="IV250_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAP8767979.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(6796..7323)
                     /gene="msrA"
                     /locus_tag="IV250_000674"
     CDS             complement(6796..7323)
                     /gene="msrA"
                     /locus_tag="IV250_000674"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP8767980.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(7407..8084)
                     /gene="pgmB"
                     /locus_tag="IV250_000675"
     CDS             complement(7407..8084)
                     /gene="pgmB"
                     /locus_tag="IV250_000675"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP8767981.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(8081..9211)
                     /locus_tag="IV250_000676"
     CDS             complement(8081..9211)
                     /locus_tag="IV250_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP8767982.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(9211..11394)
                     /locus_tag="IV250_000677"
     CDS             complement(9211..11394)
                     /locus_tag="IV250_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP8767983.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(11391..12416)
                     /locus_tag="IV250_000678"
     CDS             complement(11391..12416)
                     /locus_tag="IV250_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8767984.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(12461..13288)
                     /locus_tag="IV250_000679"
     CDS             complement(12461..13288)
                     /locus_tag="IV250_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP8767985.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(13285..14352)
                     /locus_tag="IV250_000680"
     CDS             complement(13285..14352)
                     /locus_tag="IV250_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAP8767986.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(14378..15214)
                     /locus_tag="IV250_000681"
     CDS             complement(14378..15214)
                     /locus_tag="IV250_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8767987.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(15211..16080)
                     /locus_tag="IV250_000682"
     CDS             complement(15211..16080)
                     /locus_tag="IV250_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8767988.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(16150..17433)
                     /locus_tag="IV250_000683"
     CDS             complement(16150..17433)
                     /locus_tag="IV250_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP8767989.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(17447..19132)
                     /locus_tag="IV250_000684"
     CDS             complement(17447..19132)
                     /locus_tag="IV250_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAP8767990.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGTRVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            19407..20432
                     /locus_tag="IV250_000685"
     CDS             19407..20432
                     /locus_tag="IV250_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8767991.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(21027..24680)
                     /gene="rpoC"
                     /locus_tag="IV250_000686"
     CDS             complement(21027..24680)
                     /gene="rpoC"
                     /locus_tag="IV250_000686"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAP8767992.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(24759..28376)
                     /gene="rpoB"
                     /locus_tag="IV250_000687"
     CDS             complement(24759..28376)
                     /gene="rpoB"
                     /locus_tag="IV250_000687"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP8767993.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            29104..30072
                     /locus_tag="IV250_000688"
     CDS             29104..30072
                     /locus_tag="IV250_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8767994.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            30090..30995
                     /locus_tag="IV250_000689"
     CDS             30090..30995
                     /locus_tag="IV250_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8767995.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            30992..31798
                     /locus_tag="IV250_000690"
     CDS             30992..31798
                     /locus_tag="IV250_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8767996.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            31955..32923
                     /locus_tag="IV250_000691"
     CDS             31955..32923
                     /locus_tag="IV250_000691"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAP8767997.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(33240..33764)
                     /locus_tag="IV250_000692"
     CDS             complement(33240..33764)
                     /locus_tag="IV250_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HAP8767998.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            34051..35862
                     /gene="scm"
                     /locus_tag="IV250_000693"
     CDS             34051..35862
                     /gene="scm"
                     /locus_tag="IV250_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAP8767999.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     SASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTS
                     ASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSS
                     TTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKR
                     ALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(36017..37003)
                     /locus_tag="IV250_000694"
     CDS             complement(36017..37003)
                     /locus_tag="IV250_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP8768000.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(37026..37592)
                     /locus_tag="IV250_000695"
     CDS             complement(37026..37592)
                     /locus_tag="IV250_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAP8768001.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(37606..38022)
                     /locus_tag="IV250_000696"
     CDS             complement(37606..38022)
                     /locus_tag="IV250_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAP8768002.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(38479..40413)
                     /locus_tag="IV250_000697"
     CDS             complement(38479..40413)
                     /locus_tag="IV250_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP8768003.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            40715..41650
                     /locus_tag="IV250_000698"
     CDS             40715..41650
                     /locus_tag="IV250_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAP8768004.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            41740..42843
                     /locus_tag="IV250_000699"
     CDS             41740..42843
                     /locus_tag="IV250_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAP8768005.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            42856..43809
                     /gene="menA"
                     /locus_tag="IV250_000700"
     CDS             42856..43809
                     /gene="menA"
                     /locus_tag="IV250_000700"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAP8768006.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(44744..46090)
                     /gene="gor"
                     /locus_tag="IV250_000701"
     CDS             complement(44744..46090)
                     /gene="gor"
                     /locus_tag="IV250_000701"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAP8768007.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFCGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(46224..47465)
                     /locus_tag="IV250_000702"
     CDS             complement(46224..47465)
                     /locus_tag="IV250_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP8768008.1"
                     /translation="MTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAYGLRS
                     RAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIVS
                     DPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIRK
                     NTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQERN
                     SLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKAA
                     VDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFVG
                     EVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNPM
                     MILTMPVAQPVKPGDMIRKRK"
     gene            complement(47483..48406)
                     /locus_tag="IV250_000703"
     CDS             complement(47483..48406)
                     /locus_tag="IV250_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP8768009.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(48627..51119)
                     /locus_tag="IV250_000704"
     CDS             complement(48627..51119)
                     /locus_tag="IV250_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAP8768010.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(51126..51593)
                     /locus_tag="IV250_000705"
     CDS             complement(51126..51593)
                     /locus_tag="IV250_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAP8768011.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(51728..51801)
                     /locus_tag="IV250_000706"
     tRNA            complement(51728..51801)
                     /locus_tag="IV250_000706"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(51765..51767),aa:Arg,seq:acg)
     gene            complement(51859..51932)
                     /locus_tag="IV250_000707"
     tRNA            complement(51859..51932)
                     /locus_tag="IV250_000707"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(51896..51898),aa:Pro,seq:tgg)
BASE COUNT        15074 a        10941 c         9538 g        16398 t
ORIGIN      
        1 ataatacctg ttaaagcacc ttcttgtatt tttaaagaaa tctcgttcaa tgctaaaaaa
       61 tcgttataac aaacattaag ctttttcact tctaacataa ctttctcctt ctaaatgata
      121 atgattatca cgatcatatg gtatacaaaa attatatttt agtcaaccct aaattttata
      181 gtttagattt ttttctgttg ctgaagatta gaatgacaaa actatttgaa actacgcgta
      241 tagtaagtag ctttcaaaat atataaaaaa ggagcgttct caaatgagaa catgctccta
      301 aaactacttt attggcgtat ggaaaaatta tacttgaagt ggatagcggg ttgaataagt
      361 tgataagcat caaaacattt ataaaaatta ttcaagacta tgtctgttcc atatacaaat
      421 atgaaaatca gtaaactcca tttaactttt ttcgtagtgt gtctttttat acatgtttat
      481 cgttattaat agtgaatagg aaaaaaacta tttttgatca ttgacaatta aatacaattt
      541 ttgaataacg atccctgcta ttcgagctag aaactcaagt acgccatgac ctgattagcg
      601 acaaatactt agagatttct ttttgttctg ggaaaacaaa atgccatgac aatagcagca
      661 aataatgata cttccgtaag gctcgccgta aactggggag aggtgaaatt cctatgtgct
      721 tacccaaagc aattcaaaaa tttcataact actattttag gagatgattt tatagaatcc
      781 agagtctacc tgaataaaaa acaaacctgt gcatttctag gaatcgataa taatactcta
      841 gataagtgga ttcgtatata taatttccca gtaataagag ttggtggtgt agttcgcttt
      901 gacgtagaag atattaaaga ctttatgaat acatttaaaa taacttgaaa ttttcttctc
      961 gccgcatata ctaaaattag tgaaatatac tgccttactt tttttaagga gtgaggattt
     1021 tgcctacaaa gctttcaaat gggaaatata aaactaatct gagatatcca aaaaggttta
     1081 aagaaataac aggaattgca tcagaaaagt atcaaaaaac atttccaaat aggcaattgg
     1141 cgattaaagc cgaaaatgat atgaagaaga aaatagaaaa agttttgaga gaggagaatg
     1201 caaactcatt agaattaaaa ggaaaaataa cgtttaaaaa attttacgaa tctaaatggc
     1261 ttcctagata tgaattaggt caaacaatta gatcaaacag gcctcctagt gatataacca
     1321 ttagtaatac aaaagatata tttcggttgc atattttacc tatgtttggt gaatacgcaa
     1381 tgaattatct aaatatcaat actgagataa tatcagatga attaaccaaa aaatcgaaag
     1441 aatatgcaaa tataaaaatc ataaaaggat atgttcgttc catgttcgat attgcagaaa
     1501 tactaaatta tatcgaattt aatcgaacta ctaaaattat tcaaagtatc actgcaccta
     1561 aaaaaaatgc tttagaagaa aaaagaatac aagaaggtaa gcaagcacta tcatcaaaag
     1621 aacttactaa ttggatagaa gcagtaaatg atgattttaa taatcattta cttacttttc
     1681 atgattacac actttttatg attaccctgt atcttggtga tagaaaaagt gagacttacg
     1741 ctttgcaatg gaaatatatt gattttgaaa aacaaacggt ccgtcttaaa catactttag
     1801 ataaatatca aagaaaaaag tttacaaaag gtcgaaaaga taccgtcatt caagtaccag
     1861 aagttgtaat gacattgcta agtgaatgga aatcggtaca agctgatcaa ttgttgaaat
     1921 taaaaattaa gcaaacgtta gatcagtatc tctttactta tactaaacca tctggagaag
     1981 taaattgtcc tgttcatgcc gattacctaa actatcgaat caattcaatt aaaagaaggc
     2041 atcctgactt agttcattta tctccacaca aactccgtca tacttatgca actatagcta
     2101 gacaaggtgg agcagatatg aatcaaattt caaatgcttt aacacactca gatatttcta
     2161 caacaaagat atatgttaac acaccagata tagttgataa ggctgttttt gaagcttttc
     2221 agagaggatt gaataagtgt gattgatatg tgttcttaaa tgtgttctta aaaacaaaag
     2281 aagcactttc caaataattg aaaagtgcca gaaacgttga tatataaagg atattaacgt
     2341 tttgagaatt gtgaagcttt acgtgctttc ttaagacctg gttttttacg ttcgaccata
     2401 cgtgcgtcac gagtaagtaa gccagcacgt ttaagagcta aacggaagtc aggatcaact
     2461 tctagcaatg cacgagcgat accatgacgg atagctcctg attgtccagc gtatcctccg
     2521 ccgtttacgt taacaaatac gtcatatgcg ccttttgttt cagtaacagc gaaaggttga
     2581 ttgattactt cacgcaagtc agcatgtgga atgtattctt caacatcttt tttatttaca
     2641 gtaattttgc cagttcctgg tactagacgt acgcgagcaa ctgcgttttt acgacggcct
     2701 gtgccgatat attgaacttg tgccaatgaa attccctcct attagattaa gtttgtgatg
     2761 tctaaaactt ctggttgttg tgctgcatgt ggatgttctg ctccaccgta tacgttcaat
     2821 tttgtaaatt gtttgcggcc taaagtgttt ttaggaagca taccttttac agaagtttcg
     2881 atcaaacgac gagaattttt agcacgcaat tcgccagcag tgattgattt caatcctcct
     2941 gggtagttgc tgtgacggta ataaattttg tcagtcgctt ttttacctgt taatttcact
     3001 ttgtctgcat tgatgacgat gacgaaatcg ccagtatcca aatgaggtgt gaaagttggt
     3061 ttatttttac cacgtagtac agatgcaaca actgttgaaa gacgtcccaa tggaacatca
     3121 gttgcgtcta ctacatacca tttacgttct acttcgcctg gtttggccat atatgttgta
     3181 cgcacgttat tttcctccaa tttaagttct gatgtttgac atacacttcg agtttccggg
     3241 gctctttgtg gggcaaacaa taccatttaa catattaccc gctaatctag tcaatgtcaa
     3301 cgtttttctg cttgattgaa aaatttatat gaaactttac ttcttttctt tcttttatat
     3361 aaaagggtcg tttcctgtca aaatgaaaaa caaaaagagg ctgtgacaaa agtcttgtca
     3421 caacccctta ttttgaataa acggtgttct cctgccaact cctcggtcac aagtcacaat
     3481 atccataaaa cataagaagc tgttttttaa aattgtttct tgtgactcgg agctgaacgg
     3541 caggcttaca acctcttggt aaacggtgtt caatcagctg accctcggta ttagctcaaa
     3601 aaacggcaaa atatgaggaa ctattttgac gtttgtttct ctaatactca ggtcaaaacg
     3661 ctgatttcac aacctctttt tcggtcaagt tcaacaacag tttttacgcg tcgatctctg
     3721 gcgctttgcc caaatgagct tggatcatcc agatacgttt ttctgtggct gttttgaatt
     3781 cgataaagat atcttgtgtt ggatcgtcgc cttcttctcc agaaacttcg attccttttt
     3841 ggtaaagatc tgctagataa cgatagcctg caacaagtgt ttccaaacgt tctggaatag
     3901 tacgatccca gcggccaatt tcatcaggga tttttgtgtt atctgcaaat tcacgcaatg
     3961 tagaataagg tgaaccgtct aatgtgatca agcgttcgga tacttcatct aactgagcgt
     4021 tcaaatcatc catgaactca tccatcattg ggtgcaatgt aaggaaacct ggtcctctca
     4081 tgtaccaatg tgtttggtga acaacaacag acatttggct tagatctgca actaattgat
     4141 ttaatacttc tttggtttgt tggaatttca tctttcttgc ctcctaacat tttcttacac
     4201 ttaaaataat agtagacttt caatgaaaag tacaacaaaa tcatttacct gagaacgatt
     4261 ttcagttcgc ttttttatcc gtaaagccaa tcatttttca gaatcagcta attcttctcg
     4321 cagttgagca aattgctgat aagtctgtgc taaatgcttt tgtgtcgaaa caaataatgg
     4381 atagattttc tgatattttt tatgatcagt ttcttgtact ccaacttgct ctttcaattc
     4441 tatcaattcc tgtgcttcac tgatactttg gatctttccg atgcttctcc agcctaaaat
     4501 cacagctcct aatgcggatg cttctgtgac agtagggaaa acaagcgggt aacccagtac
     4561 atcagccatc atctgacgaa aaatcggaga ttgagcaaac ccgcctgtag cacggatctc
     4621 tgtgagctga ttttcctggc aaactccttt taaaatccga taaaggttga gacagatccc
     4681 ttctagaatc gccttcatca tttctttttc tgtatgttgc cgctgtaaac cgataaaact
     4741 gccaaacgca tctgcgtccc aaagcggtgc tcgctcacca agtaaatagg gatgaaaaat
     4801 cagaccattg gcacccgctg gcacaaaagc gatagccgaa agcagtgcat catatggatt
     4861 ttctcctttt tctttagcag attgaacgac ttcctgtaaa aatgtatgcc ctgcccaatc
     4921 aaagatgcca gcaccattgc tagtcgctcc tcccactacc caatgtttct gatccagaac
     4981 ataacaaaac gtctccattt ctggatggaa ctgaggctga gtagtaataa aacgaagtgc
     5041 tccgctcgtt cctacggtca aagcggcgat cgtatcacca aaagcaccta accctagatt
     5101 agaaagcggc ccgtctgccc ctcccaaaac aaatgatata tcatttggca aacctagttc
     5161 tgctgctgtt tttgatggta gacctgttac ttgataggtg gaaggaaaga cttgcggcaa
     5221 ttgctctatt cgaatgttca agtagtcaag cacttcttct gcccattgga attcatggat
     5281 atcaaaatat ccagttcctg aagctgttcc gtagtcgctg atcagttcgc caaacaatct
     5341 ataaaagata tagtctttga taccgacaaa ataagcagtt tttttgaata attcaggttg
     5401 gctgttttgc agccacttga ttttgttcaa tggtgtcatt ggatgaatcg gcgtcccagt
     5461 ctgccgataa aattctttgc caagcacggt atatttcagt ttttctgctt cgtccgctgc
     5521 tcggttatct gcccaagtga tcattcgaga taacggtgtt ttctcttcat ccaacaatat
     5581 caggccatgc atcgcagagg agaaggagac aaattgaatg attgtgtctg aaggcaactg
     5641 tttaattact tcttttataa cggtgcagac accttgaaag atcaattcct gatcttgttc
     5701 tgccatccct tgtgtttctt ggatcaaagg gtagcgaaaa taagcggatg ctgcttcttg
     5761 ggcttcatct gtgaaagcaa ctgctttggt ttgtgtggtt ccgatatcta atccgattga
     5821 aacgttcatt ttttcacctc aaattttatg tatgtacaaa aatttagttt aagcttgcgt
     5881 atcttggttt tgaaaagggg gagatggcgt gttactttat tttatcattg gttgctgtat
     5941 cggatcattt ttatgtttaa ccgcccagcg gattccgtta ggtcactcta ttatttatcc
     6001 tagatctcat tgtgtcaaat gctgtcgttc gctttcttgg tatgaattga ttccgattct
     6061 ctcgatcatc gtccaacgat ttcgctgccg ctattgcagg tgtcggttgc ccttctacta
     6121 tttgttggca gaagccttat gtggtggtct ttttgcctgg ttctttactt tttcttccgc
     6181 aagaaatttt tcgacgttca tatggatgct ttcggctttg ctattttcgt tgatggattt
     6241 attttacttc atggtccatt ctcacacgct attctgttct tgggcaattt tatggatttt
     6301 ttggttgcag gccggcgtct tccaatggca aagtgtgctg ttaacgttta tcgtaggcgg
     6361 tgtgtgcctt cgctatggtc aatcctatct cggagctggc gatactctgt tgatgctttc
     6421 ttggagtggt ggcttgtctc tagaagagtt gcttcagatc ttgtttcttg ctagtctgtt
     6481 tggaatatcc gttttttctg tttactttat ctgtcataaa aaaaagttgg aaaagctgcc
     6541 tttcattcct tttttgagca gtgcgttgct gattgtcctt catcttaggt attgaaaaaa
     6601 ggttgtgaca aacgacttgt cacaacctct gatatatagt gttctcttac caaatccttg
     6661 tgacttggag ctaggcgaca ggttcacaac ctctccacta aatggtgttc aatcagctga
     6721 ccctcagtat tagctcaaaa tgctggtttt acaacctctg atatacggtg ttccataagt
     6781 agctttttcg gcttcttatt tcttgacgcc ccaatgtcga cgaaggaaac ctgcacgtcc
     6841 agaaccttct cgataaagat tgtaatggag cttgtttttc ttgtaataat cctgatggta
     6901 atcttctgca ggataaaatt cgcttcttgg ttcgattttt gtcacgattg gcttatcgaa
     6961 tcgcccgctg tcttgcaatg cttgttttga acgctctgca atatttcgtt cttcttcatc
     7021 attatagtag atcactggac gataagaatc accgcgatca gcgaactgtc ctaatgcgtc
     7081 agtcggatcg gtttgctgcc aatagatttc caccaactga tcataagaaa taacttctgg
     7141 atcatattcg atctctactg cttctgtatg ccctgttgtg cctgttgtta cttgttcata
     7201 agttggattt ggtacatgcc cgccagtata accggatgtc acagaaatga tccctggctg
     7261 ctcttcaaat ggtttgacca tacaccaaaa gcatccgcct gcgaaaactg ctttttttgt
     7321 catataaaat cccccttttt tctcatcata ccttgatttc caaaaggagg ataacgtttt
     7381 gcttgtgaca gaaaaaacgg aaactattat atacgtgata cggcttctga taaatgccaa
     7441 aacgaacgta cttgatcaaa aacggttaaa tcaggaatca catattcagc agaaggaaga
     7501 tttatgtttt cacctattcc caatacatgc atgttaccag ccaaagctgc ttgacaacca
     7561 gcttctgaat cttcaatgac caagcatgca tttggcggta cattcaattg ctgtgctcct
     7621 ttgagaaaaa cttctggatc aggttttgct tttgatactt gcgtgccgtc aataaggaca
     7681 tcaaaataac ttgtcaaatt cgttttttct aaaatgaggc gtgcattttt gcttgctgat
     7741 cccaatccga tcttgatgtt tgtttttttc aagtactcaa gaacagctat tgcaccaggc
     7801 aaaacggatg tttcatccat tttttgaatc gcttgaagat atagattgtt tttttgctca
     7861 gcaaaggctg atttctcttt ttctgagaat ttctgttcca tttttccaaa tgacagcaat
     7921 cgctctagtg actcggttct ggatattcct ttcagttgct cgttttcttt ctcagtgaat
     7981 ggaatggaca gctggttagc taaaaccagc cacgcttcgt aatgataacg agctgtgtct
     8041 acaagaacgc catctaaatc gaatagtgct gctttgattt tcatcgatta cctccttgaa
     8101 aatgaacgac ttgaagtaaa gatgcgatgt ttttagctgt gcgatgaaga ttatcagtgg
     8161 cttgctgtaa agcttcttgt aaaggcacag ctgcaggaag gatcggaaaa acggccgaaa
     8221 tgccaacatc tgctgctttt tcgcttcctt taccaacact tccgcaaata gctatcacag
     8281 gaacgccctc tggtactctt ctagcaatcc caactggcgc ttttcctttt agactttgtc
     8341 catctagacg tccttcgcct acgattacga ggtctgcttg ctgtacccgt ttatcgaact
     8401 ccagtaaatc caatacgtag tcgatgccag agactatttt ccctgaagca aatgctaaca
     8461 gtcctgcacc cattccacca ccagcactac tgcccgatgc ctcgattact tgaggggcaa
     8521 atctcatata aaaatcatgc atggcttggt cagccactgc taattcattt tctttcaagc
     8581 ctttttgtgg tccgaaaaca aaagaagcgc cttcttttcc gcataaaaga ttagtaacat
     8641 cagtaatgac agtgattttg gctttaatca gctcaatagg cacgttcgtt ttatcaactg
     8701 tagcaatttt acctagattt tctcccagtg cttctacttt ttgattatct tgatcgtaaa
     8761 aagcataacc tagaccttct gccatgccga tgccgccatc gttggtacta cttccgccaa
     8821 cgccgatgat gatgtgtcga acaccttgtt gcaacaattg tcgtatcagt tctcctactc
     8881 ctcgcgtgga taacctaagt ggctgttgtt ggtttagtgg gacatctttc aatccgacga
     8941 tttctgccat ttcaaaaatc gcagtttgac cgtctgaagc gtactggatt tctttgtttt
     9001 tgccaaatgc attagtaacg actgctgttt gccgcttcaa ccttaaactg tctgctaatg
     9061 atgaaaccgt tccttctcct ccatcgccta taggtagcag atcatattca gcctgaacaa
     9121 aaacttcttg aaaacctgat tgaatggctt gtgctgcctc taaggctgtc agactttctt
     9181 tgaaagaatc tggcgcaatc agaatacgca ttattttccc ctccgttcca agatcagttc
     9241 tttttccagc tggtattggt ctccattgac aaaaataggt aatgtttctt tcgtctcttt
     9301 ttttattact atttgttttt ttgtcaaaac aaatagcaac ctgtgatttt tccaaataaa
     9361 aggaaaagaa attttttccc atgctgaagg aagtcttgga gaaatagaaa gagttccatt
     9421 cgttactgta atacctgaaa aaccaaatat aactgctagc catagtgctc ctaatgaagc
     9481 agcatgaatt ccgtcatctg atgaatgcgg atctggtcct aaatcaatca ggcacgcttc
     9541 ttggaataaa tgatacgccc attccatttc ttggatacgg caagcctcta tggcgtgaat
     9601 cgctttactc aacgatgagt cgtggattgt tttgttttca tagtaatgca aattcttaga
     9661 aacgattggt tgagaaaaat actcaggtaa taagtataac agcatgacaa gatctgcttg
     9721 cttgctgact tggagttcat ttacttcgct acgggcataa tcaagtaaaa tagactggct
     9781 gccaggacgt tctttgtaag tggtcaaatc aataactggc ttactgagat acgtgtcatc
     9841 ttgcggaatc agctgttctt tgttaggcac tggcaaatat aacttatcta aaaaattttg
     9901 acattctttc aaaaaaagcg gatcgccagc ttgatattta acagctaagg ccacattata
     9961 atgcgccaga tagttcgtat atgcattatt gtcgatatat tctgtgtatt catctggacc
    10021 aatgactctg tgtatctcta aatgatcatc gacttttaca gctctgctca gccaaaaagc
    10081 tgctgtttca tgaatgagcg tttttccctt tgtttctaaa aacgattgat cctctgttgc
    10141 ttggacatac tcttgaacgg cataagcaag gtctgcactg atatgatgtt ctgcggcagc
    10201 tgaagcaact tcttgtcttg ttccggtttt gatattcaat gcagcaaatt tgggtgtctc
    10261 ttcctgacca gtgtgtgcgc tttcccatgg gaaaagtgca ccttgatagt gataatctct
    10321 tgctttttgc cgtgcgcctt ctaatcgatc ataacggtac agcagcaaat tctttgctac
    10381 ttcaggaaac tgatataaaa agaaaggcag gataaaaatt tccgtatccc agaaaacatg
    10441 ccctttataa ccttcgccag ttagtccctt cgcccctaca ctaacacttg gatcattgcg
    10501 gggagtcata atctgtaaat ggtaagcagc gaagtccatc gctaactgat cgaaagaaga
    10561 atcgccttgg atcgttatcc gatgctgatg ccaaaaatct gtccaataag acatcgactg
    10621 ctctaaaatt tctgtataac taacgctttt ctgggcttgg gcaatacttt tcttaaacgg
    10681 gtctgtttgc tgaagagagg tagagagcac gatgatcttc tccagcgtaa aggatgtatt
    10741 ttttctgatc ttttctgaaa aggaagcggt gatccggcga ttttttgctt ggaatacacc
    10801 tgctttattg aatttagctg caatcccaat atcaatttta ctttctgttg tctggtagga
    10861 tgcactgatc aaatcgtcac caaatactcg cagttctttt tcaaccaact gctgtgtacc
    10921 aaaattggtt tgctgtccat cgatacccgt tttcacatct acttggcagt cttcggtcaa
    10981 cggcttgatg accactttca gggctgcctg atggagatta ttttgatcag caacacgaca
    11041 agtaatgact tccacttttt ttccttgagc agtttcaatg agcgcatagc gtttaagctc
    11101 tcccatttct aaatctagtg cccgctcgta tgcaagaact ttcatcgtct caagagaaaa
    11161 atgttcaccg tcgattttta tttcccatct gatgacatct gggagattca ctaactcggc
    11221 tgattctgct cctaaaggtc ggttatatac accagctgca aacattcccc ttacttgatt
    11281 ccgatagtct tcctcctggc ttcctctgat accaaaaaat ccatttccaa tggtcatctg
    11341 actcgcatac ttattgatat actctaaatc aaactgtttt tcttctactt tcataaggta
    11401 ataacttctc ctgtttctgc cgaacgatat aaattttcca ccagattctg gatgacatat
    11461 ccttgccagc catctgcaat catctgagtc tggtcaccga gcgcccgatt cacaaaatga
    11521 gccatactta gttcatgtgg gtcattaact ggcgtggtct tttgtaagga aaccagcttt
    11581 ccatctacat cttcgaatag ttcgcctgga tacagattag cacctgcctg atcgccacaa
    11641 aaagccacat tcagtttgtc ttctctgata ttcaaagcaa acgaagtatt cagttggatg
    11701 atcttgccgt tttctaattc cagtgtgcca aaaattgcat cttctactgt gaatttgctt
    11761 ggatcccatt tcccgaattt ccctgaattt tttttcgtac caattttttg gaagctgtac
    11821 gcccgaacgc tttttacttt aggaaatcca gtaatataca tcgctgtatc aagcatgtga
    11881 atgccataat cgatcaaagg cccgcctcct tgaagttctt tgtcgataaa cgttccccag
    11941 cctggaacac cagaccgacg aagcgcattc gcttctacgt aataaatatc cccgagaaca
    12001 tcaagatgat tcttgatcat tgttgcttcg tcagaaaaac gatgctggaa tccataagcc
    12061 aaaatctgcc cgtttttatc ggctgtctct gccatctttc ttgcttgatc agctgtcata
    12121 gctggtggtt tctcgcacat cactgaagca ttagcttcga gggcagccaa tacatgttcg
    12181 aagtgaaagc gattgggtgt acagacactc acgatatcgg gagaaatctc attcagcatt
    12241 tgtgtaactg atgtatacgc atgagaaaat cctgtacgtc ggcaaaagtc ttctgctcgc
    12301 tggctgactg gatcaaccac agcggccaat tctaagacat ctgatctttt ttgataataa
    12361 gcagcatgta ctttttctgc tacttgtcca gcgcctatga ttgctacttt cttcatttat
    12421 ttctccctcc tttgcttatt tacttattgt tattctttct ctattctttc tctaaatcat
    12481 ttaaatgacg cgctgtttcc agacagtttt tcaagtattg caatgattcc tgataaattt
    12541 tcacttcatt ttctcctcgc aaacgacatt catataccat ataaccttca tactcatttt
    12601 gaagtagcgt ttgaaaatgg cgagtaaaat caattgcgcc agatcctggt tggtagcgat
    12661 gattatctgc taaatggata tgacccacat actctttttg cgtagtcaat gcttttgaaa
    12721 tatcatcttc ttcaatattc atatgataaa aatcagcgat gatctgcaca tgtttcaatt
    12781 gattagagtc aatgtaatga cgcgcatccg ctaacaaatt gatcatatgg tcttggtatc
    12841 ggttcaaagg ctccaaataa ataaccgtac ctgtttcttc ggctacttta tctaaataac
    12901 gaagtgattc gctgactgct ttatagtctc cttctttgct tcggggtgaa accatcggcg
    12961 gtaatcgata agtgaacatt ccccaagctg ctgggataac gattcctttg cctccaacct
    13021 cgcttagtgc acgcaaaatc tcagcaattt gacgtacacc attaagacgc cgttcttcga
    13081 taaagtcacc gatccaccca tcgtagccgt tacaagctgt cactactggg atccctgttt
    13141 ctttgattgc ctgtttgact gtgtcgatat tttctattaa caacttaccg tcgatttcgt
    13201 attcttcaaa tccacagtct ttgataaatc taaatttttc taaaatgtct cttgggaaaa
    13261 atgcttgatc ttgcgtaccg aatttcattg ttctcctcct cggacttgaa cgcccatttt
    13321 gatactttct tctgggtgct ggtctacata acgcatgtaa gcttctgcgc tttctgcaaa
    13381 tggcacaact ggatcaatga tttcttcaca atccaagtaa ccgttcatca ataattccca
    13441 acatgtgtcc tcgattcgtt tacggttcca tcttgggtaa tctgggtttg gttcactaga
    13501 tgctcgagca aagacgattt ttgcattatt gaaatgtgct tcgcgaccaa gattaaaccc
    13561 agctggaaaa ggtttagcaa aggcaacata agaaatgatc ccgccatagc cgatccctct
    13621 taaagaagct tgtaatgcgg cagcactgcc actcgtttca ataactgaat ctactccacg
    13681 tttatctgtc aattccttta gacgaaaacc gacatctgct ttcataggat ctagcgtgta
    13741 atcggcaccg tatttttctg ctaattctct acgctgcgct atgggatcga tcccaacgat
    13801 ggtagtagca cctgcttttt tagctaattg aatcgcaatt tgcccgatag cgcctaaacc
    13861 gaagattgct acagcatcac ctgctcgaac atgtgcttcc cggataccac ctaaagcgaa
    13921 ttgagcagga tcgtaacata ctgcattttt ccattgatcc aaccgatcaa gtttacgtag
    13981 tttataatta tcaacaccgt ttgtaatcag cgtttcacga atgggtccat aagtgcagac
    14041 taactctcct ttttgatact ctgtcaccaa actgcccgtt tcttcgatcg taccgacaat
    14101 catatttcct aattgaaatt cgccaaatac cacaccgcgt tggcttcctt cttctcttgg
    14161 caagaatagg cgccattctt ctgaaaattc ttcatcgata aatggactta atcctctaaa
    14221 gtcgacaatt tctgttcctg gttttggagt agcgaaaatt actcggatac gtacttcgtt
    14281 cttttcgatc tcacggtctt cgtaatcttt gaatccggct attcttggtt ctaatgcaac
    14341 taattttttc atcttttctt tcctctttct tttcttttta tccttttacg ccgccttcga
    14401 ctaaatcacc tttaataaat cgttcagata acgcatacat aatgatgact ggtaaagcgg
    14461 tcaccaatga agcggccatc attcgtcccc aaatgtagtc tggtgtactg aataatgtgt
    14521 tcaaaccgat tggtaaagta aatttttcgg aggtcgacag gaaaatcgaa gcaaataaat
    14581 aatcattcca cgaaaccata aagcaataaa cgaagacaga aatgattcca gaaatagcca
    14641 atggtataat gatccgaata atgatctgga tccggctaag accatcgatc attgctgctt
    14701 cttcaatatc agtcggaatc gtgtcaaaat aactttttag catgaatatc gcagtaggaa
    14761 gtgtttgaac gaccatcgtg ataatcagtg ctgtattggt atcatacaaa cctagaccgg
    14821 aaatgatttt aaataatgga acaacgatca aaataccgga aaacatataa acggtgtaaa
    14881 agctagcatt gattttcgag cgagctttga agcgtagttt tgacaaagcg tatgccccgc
    14941 tgatcccaat cactactgca gttacggagg ctatcaagga gatcactaaa ctatttttga
    15001 aatacatgat gaatggaaaa atctcagcat tgaaaatatc ctgataatgt tcaaaagtcc
    15061 attctttcgg aaaaatagtt tgtgtcgttg caattgcttc ttgtgaatcc ttgaatgatg
    15121 tcatcagcat gatcaaaaat ggaaacaaat tgacagctaa tagcaacaaa atcgtcgcgt
    15181 aaaataagat tttcctgctc tttttatttt tcatttcaag tccaccttct ttcgtgagag
    15241 caagacgacc gctgcaataa tgatgaacaa tagaatcgat atagctgctg cttttccaag
    15301 atcattgaac gcaaaggctg tctgatacaa ataaacgcct aagatattga ctttattcgt
    15361 cagtagatag acatctgtaa acatatagaa catccagata gatcgtaaaa caatcacagt
    15421 tgctagtact ggtttgattg ccggcagtgt cacgatcttg aacttctgcc aagcatttgc
    15481 accgtccatt tcagctgctt cataaagcgt tttgtcgact gtttgcaaaa tagccaaaaa
    15541 tgaaataaac gcatacggat agtatctcca gatagcaaac aaaacgacca taaagaagct
    15601 agcaactggc tggtcgaacc acagcggtgc ttcgttgaaa agctgcaacc tatctactaa
    15661 cagatcattg acgattccat aactattatt gaacatatat ttccaagcaa aaatcaaaga
    15721 aatagatggc gtaacataac ttaataaaat cagtgaacgc accgttttcc taaatctgaa
    15781 cttgcggtta agcaaaatag ctacggctaa tcctaaagct gtactgcctg ctacaactaa
    15841 gaatgtgtaa gcaactgtta aacctaaaga acgataaaat tcactatcac ttaaaatttc
    15901 aagataattg cttaatccga taaaaacggc gtctgagtta ggattcagcg gcatatcgaa
    15961 aaaactgatc tgaatattgg aaagcattgg aatcagcact aaaacagcta ataaaagcat
    16021 gcttggtgct aaaagtccca tggctaattt taagtcagaa tgtttccttg gacgattcat
    16081 tgaaacctcc ccctgataat ctgccaatag ctatctactt ttttcgaaca ccattggcag
    16141 aacttcttgt tatttcgtat ttttttgtgc gtcagctaaa gcttgatcga catcttcgtt
    16201 tccgacagtt acttggttca ctgcttttcc gatcacacct gagcttgtga tatctcccat
    16261 tttcgtgaaa ttcttttccc cgactagtcc aaagacttgt acttcatcaa aagcactagc
    16321 aatctcagat ggcagctcgc caaaagactt gatgacttca ttttctttgt aaccatccag
    16381 ttcgactact tgtttattca ctggttgagc accaccagga gacatcaaga cccacttttc
    16441 catgtttttc ggttgagata agaaagctgt gaatgcttca gcagcctctt tttgtttctc
    16501 gtctagacca gaagaaatcg tcaatccaga tactgtgcca tagacagctt gatctttttg
    16561 tgttgggata gcaaaaccga tattttctga gttccccgct tcataaacag aaggcaaaat
    16621 gtaagttgaa tagattgcca ttggtgctgt tccattcatg aaagcatctt tgatttctgt
    16681 cacatcatta gagcctggca tcgtgtattg ggataagttt tgataaaacg acaatgcttc
    16741 tctcatcttt tccgtatcga tcgttacctc gccttcatca tccaaaacat ttgctccatt
    16801 tgacaaagca aattgagaga acgcttgctc actcattgtt ccttcagcag taggcatagc
    16861 aattccatat tgtttattct ctttatctgt gaaatgttga gcaattttta aaatatcatc
    16921 ccaattttct ggttctgaaa aaccagcttc tgataatttt tctttgtcat accaaatacc
    16981 ttgaacccag ccgctaattg gtgcagcaat atagcttttt ccatcttctg aacgaacaag
    17041 gtttttggct ccgtcataat aattatcttc tccagcttcc tcgataacgt tttggactgc
    17101 cgtctgatca atcagttcat ctttatccat cactttagca aaatcctgac tgacttccat
    17161 tacttctggt aatttaccgg aacgagctaa cgtaacgatt tttgtattga acgctgattc
    17221 ttctaccggc acttgcttga ttttgatgtc tggattttct ttttcaaaat cagcaaccag
    17281 ttcattgatc acacttaatc gttcctgttc gacagaagag tgcatgaatt gaatcgttgt
    17341 ctgttgtgat tcgttagagc tttgactttc accacaagca gtaagaagaa atccagtagc
    17401 tagcagacag gtagtaacaa taattttttt catttttttc cacctcttat tttattattt
    17461 tcagccacat atattcatac ggctggaata tcaactttcg ttcttgactg caatggataa
    17521 tttgtccaga aaacaggtct aggtaatctc cttttggtaa aagataacct gctgattgat
    17581 tggaaaaatt atggataact aagactttct cttccttcgc ttcgcgaata aaactgataa
    17641 aagaatctgc tgagtccagt aagtaaatca aactattcgg atggaaggct ttttcttttt
    17701 ttcgaataga tagcatctgg gtcaattcac catatactgc tgttcttaat ggatctgtct
    17761 gcaactcttt ctcaatatct gctaacaaaa atttttgccg attgattttt cgattctctc
    17821 ctgttttttc tacgcctttg taatcatttc gacttcccag cacactttga atataaattg
    17881 ccggcacgcc tggtaacgtt accaaaaatg aatgagcagc taaaaagcgt ttgcttctcc
    17941 attccgtcgt atctgttgga gaacttaacg catctagata cgtagcgttt atctcatatg
    18001 gactggtcgt tccatctggg tttttcttgt agtttactcg ggtaccctct ttcttcagct
    18061 gttccactaa ttcaaggatc gttttttccg gcagaatacc tcgtaccgga ttcaatccga
    18121 taccgtcatg ggaggctaaa aaattaaaaa aagttgtttt tttcgatgcg aaatcgatgg
    18181 tttttaacca ttctttcaac ttacttgtat tacccgaacg aatcgcttga agggtcaaag
    18241 gtggtagcga aaactgatag accatctgtg cttcattctc gccattccca aaataactga
    18301 tattatcttt gtgtggcaca tttgtctcag tgatcaatac cgttcctgga gctgcttcat
    18361 cagcgattgc acggaataat tggatgattt tatgcgtttc tggcaggtgt atacaagaag
    18421 ttcctagttc tttccataaa aatcccacag cgtccaagcg aataaatgaa gcgccttttt
    18481 ctaagtaaaa caaaagaata tcgatcattt tcaataaaac ttctggattt ttaaaattca
    18541 agtctacttg atcttcgcta aaagtcgtcc atacgtgttt ttctttcccg tttttgtcaa
    18601 tgaacgttgt aagtaacggg ctggtccttg gtctagtcac gctgcttgta tctgtatcca
    18661 gtggcaaatc aataaaaaaa tctttgtacg tatcattcaa gtttaaatat tcctgaaacc
    18721 aagtactttc ggcagacata tgattgcaga caaagtcgaa cattaaccgt gtttcgcttc
    18781 tcatttcttc tatatcttcc catgtgccca aacggtcgtt gattgcctga taatttacaa
    18841 cggagaaacc atcatcagat gtatatggga aaaatggcaa gaaatgtaca actctaaatg
    18901 tatctgccaa atacttttga taaaatttct taaatgtacg caaagttggt tgctgctctt
    18961 caaaaaattg atccccataa gtaatcaaga aaatatcttc ttcgtcccaa tgcagtttgc
    19021 ctgcctggcc tttcttctgc tgataaacac tgactttttg ttcaatcata gaggcggttt
    19081 tctcagacaa atctgagtcg cgataaatcg cttgtaaaag ttttgtatac atcggattcc
    19141 cctttctttt ttgggagcgt tcccaacgat ttgatcatat tatggaaaac gcttactgtc
    19201 aatagcattt ttttaaataa ttttgatata cacaaaaata caaataagaa agcttatgta
    19261 tcaataatta atagcttctt aaaaaaatat ggtctctcat gatcatgcaa gctattccta
    19321 aattttcaaa atcatagaca aaaaagaatg tctattgtac tatggaatta ttctaaaacg
    19381 ctcccaaaaa ggaggtgcat ataaaattga aacctactat ttatgacatt gcgaaaaaag
    19441 ctaatgtctc aaaatcaact gtctccagag tattaaataa ccagccaaat atctctgata
    19501 aagcaaaagc aagtgtactt caagcaatca aagaattgaa ttatgaaccg agcaaaattg
    19561 caagaggact gtcaggaaat ggttttgacg cgattctagc attatctcat cgtacttctc
    19621 atacgaccta tggcaatcct tttttttcag atgttattca atccatttct gccgtttctg
    19681 aacaaaataa cttcgacttg attctacaaa cggcaaaaac accagaagaa gagctagaaa
    19741 aatgcgcgaa aaagatccaa gaaaaaatga tccgaggaat tattatttta agctctacga
    19801 tggatgaaac acttcttttt gaattggatc aatacaatat tcctattgtg gtaatcggaa
    19861 aagtcggaaa cgactacacg aacatctact cagtggacac ggataatttc caggatagtt
    19921 acgatttggt ggaaacactg atcaagaatg gacaccgtca tatcggctgt ctccatgctc
    19981 cgacagaagt ttatgtatcc aatgatcgtc tcaatggtta ccgtcaatgc ttatttgatc
    20041 accacctaga tatccgtaat gaatggatta tagacggcgg ctttcgctta gaagatagca
    20101 tggcagcaat gaatgattta atgaagctac gagaaagacc aactgcgatt ttttctaccg
    20161 atgcattaaa atcattgagt atttacaaag tggcagaaca atctggaaag cagatacctg
    20221 atgatctttc gatcgcagga ttcagtgatc ccttattgtc ctccctattt tcgccacagt
    20281 tgactcggat agacattccg acaaatcagt taggaatcaa agccacagaa cttttattcc
    20341 aactaatcca ccaccaatac cctgaagaaa aaagaatcat tctttcaacc gagattactc
    20401 tcacagattc gatccaacaa caagaaatgt agaacagaat tataaaagaa cagaaacact
    20461 gaggataagt cagttactct gttctttttt caatcactca tccctcgtta ccccctcccg
    20521 gtattttagc gaatttttct tattttccat ctgttttaga tagctgtgga taactataat
    20581 gtttgtgtta aactattttt tccacaggga agaaaaaact atttttaacc acgttgtgta
    20641 taagttttta tagttcgttt ttttaaacac atttaccctt atttcaatcc atgttatttg
    20701 gggatactta tctctaaaac ataaatagaa taacagacat gtctactggt tttgttttat
    20761 tcgaggaatt attcaataat agctagcaaa atattaaaaa taaccgctct atcttcccat
    20821 caatttattt tgcattcaaa aaatgatcta tctttacttt tgttgattga cttctcttgg
    20881 aagatgatcg ttccgatacc ttaattggat acgaaccgat gttgttaaga tcctttccca
    20941 cttttcttct agttagacaa attattttag acaaaccaaa aaagaggttg tgacaataat
    21001 ttgtcacaac ctctttttta aaatatctat tttgtttgat tgttcattgc gtcttctgca
    21061 gccatttgcg cttcaatatc tgaaatactg tagacatttt cgcttgctac accgacttct
    21121 tttggttcca tgtcacgata acgttgcata ccagtaccag ctgggatgat cttacctata
    21181 ataacatttt ctttcaaacc aagaagagga tctttcttac cgcggatcgc agcatccgtc
    21241 aagacacgag ttgtttcttg gaatgatgca gcagataaga aactatttgt ttctaatgat
    21301 gcttttgtga tacctagtaa gacaggtcga gaagttgcag gaacgcctcc tgagactagc
    21361 gtattgtagt tttgatcttt aaagtctgcg atgtctaata atgtacctgg taagatatct
    21421 gtatcacctg gatccattac acgaacttta cgcagcattt gacgaaccat tacttcgata
    21481 tgcttgtcgc cgatttctac cccttgcata cggtaaacac gttgtacttc acgaagcaag
    21541 tagttttcaa cagataatac atcgcgaact tgcaacaatt gttttggatc gattgatcct
    21601 tctgtcaatg gtgcaccacg gtggatcata tcgccttctg ctactttcat acgtgctgta
    21661 taaggaacag tatacgtacg tgtatctgtt tttcctttga ttgttacttc tttggtacgt
    21721 gttgcaggat cttctgagat atcgatgaca tcccctgtta cttctgtgat cactgcttgc
    21781 cctttcggat tacgtgcttc aaagatttct tggacacgag gaagaccttg agtgatatcg
    21841 tctccggcaa ccccacctgt atggaatgta cgcatcgtca actgagtacc aggttcaccg
    21901 attgattgag cggcgatcgt accaactgct tctccgactt caacgtcaga gccagttgca
    21961 aggttgcgtc cgtaacaatg tttgcagaca ccatgttttg tattacatgt gaagacagaa
    22021 cggattgtca ctgtttcgac acctgcatca acgatttgtt ttgcgatgtc ttcggaaatc
    22081 aattggtttc ctgcaatgat caattcattt gtttcaggat gacgtacttc tttacgggtg
    22141 taacgtccca ctaaacgatc ttctaatgat tcgatgattt catttccctc gcggatggct
    22201 tggatttcaa gacctcggtc agtaccgcaa tcttcttcac ggatgattac atcttgggcc
    22261 acgtcaacca gacgacgagt caagtaacct gaatcggcag tcttcaaggc tgtatcggtc
    22321 atccctttac gagccccgtg agttgagata aacatttcca agacggatag tccttcacgg
    22381 aagttcgaaa tgattggcaa ttccatgatt cgaccattag gagcggccat caaaccacgc
    22441 attccggcaa gctgtgtaaa gttggaaatg ttaccacgag ctccagaatc agacatcatg
    22501 aagattggat ttttcgcttc caaaccttcc atcagttttt gttggatgct gtcttttgca
    22561 gcattccaaa cagcaatgac acgttcataa cgttcgtcat ctgtgatcag accacgacgg
    22621 aattgttttg taatagtttc aacttgttta tgggcatttt caatgatttc ttgtttttca
    22681 ttcagtacac tgatgtcagc gatccctact gtgataccgg catgtgtcga atgtttgtac
    22741 cctaagtctt tcatacggtc aagcatctta gaagtttctg ttactttgaa tcgtttgaag
    22801 acttctgcga tgatgtttcc taagtttttc ttcttgaacg gtaatactaa ttcttgctct
    22861 ttgatatgtg caggaatatc agtaccagct tcaacaaagt atttgtccgg tgtttggacg
    22921 gtcaagttga agtctgttgg ttcattcaga taagggaatt caggaggcat gatctcgttg
    22981 aagatgatct taccaactgt tgtgatcatc gtacgttctt tttgccattc agtaaatggt
    23041 ttttcaccta atgagttagg gttcacaccg atacgtgaat gtaaatgaac atatccgttg
    23101 cgccatgcga taacggcttc gttttgatct ctgaagacca ttccttcgcc ttcgcgtcct
    23161 tcttcttcca ttgtcaagta gtagttacct agaaccatat cttgggaagg agtaacaact
    23221 ggtttaccat ctttagggtt caagatgttt tgagcagcta acatcaacat acgtgcttct
    23281 gcttgtgctt cttcattcaa tggaacgtgg acagccattt ggtctccatc gaaatcggca
    23341 ttataagctt cacatacaag tggatgcaaa cggattgcgc gtccttgtac taaaactggt
    23401 tcgaatgctt ggataccaag tcggtgaagc gtaggtgccc ggttcagcaa tactgggtgt
    23461 tccttgatta cgtcttctaa aacatcccat acttcgtctt cttgacgttc gattttacgt
    23521 ttcgcatttt tgatgtttga agctagttcg cgttgaacaa gctcacgcat cacgaatggt
    23581 ttgaataatt caatggccat ttcttttgga agtccacatt ggtacatctt caagaaaggt
    23641 ccaacaacga taacggaacg acctgagtag tcgacacgtt tacctagcaa gttttgacgg
    23701 aaacgacctt gtttcccttt caacatatga gaaagagatt tcaatgggcg gttacctggt
    23761 ccagtaactg gacgtccgcg acgaccgtta tcgatcaatg catctactgc ttcttgcagc
    23821 atacgttttt cattttgaac gatgattcca ggtgcattca agtctaacaa acgtttcaaa
    23881 cggttattac ggttgattac acgacggtaa agatcattca agtcacttgt tgcaaaacgt
    23941 ccaccttcta attgaaccat tgggcgtaaa tctggtggaa taacagggat aacgtccata
    24001 accatccagc ttggttgatt tcctgaagcg cggaatgctt ctaaaatatc caaacggcgg
    24061 attgcacgtg tacgtttttg tccttgtgca gatttcaatt cttctttcaa ttcggctgct
    24121 tctgcatcta gatcaacatt atcaagtaat tgtttaatcg cttctgcacc catagcagct
    24181 tggaattctt gtccatattg ttcacgtttt tcgcgatatt cgcgttctgt caacaattgt
    24241 tttttctcta aattcgtatt tcctggttcg atgactacat atgaagcaaa ataaatgatt
    24301 tcttccaaag cgcgtgggct catgtctaat acaagaccca tacgtgaagg aatcccttta
    24361 aagtaccaga tatgtgaaac aggagctgct aattcaatat gtcccatgcg ttcacggcga
    24421 actttcgaac gagtaacttc cactccgcat cgatcacaaa cgattccttt ataacggata
    24481 cgtttgtatt ttccgcaggc acattcccag tcttttgtag gaccaaaaat ccgctcacag
    24541 aacaaccctt cgcgttcagg ttttaaggta cgatagttaa tcgtctcagg tttttttact
    24601 tcaccgtaag accagcttct gattttttcg ggagaagcca aacctatttg catactttcg
    24661 aatttattta catcgatcaa aaggggttcc ctccatttca tttaatggca aaccgcaaga
    24721 cggctgatct tcgaccgcct tgcagctcat agaccggatt agtctaaatt atcttctgcg
    24781 gtttcaaagt tttggatgac atcgtctttt tcatcttcca cttgttcagc agctttcttt
    24841 tctaattctt tggcagtttg ctgttcggca aattttgtca aagcatcaac agtgatcaaa
    24901 tcgtcgtctt catcgtccat gtcgcgaagt tcgatctctg tttcttcgat atccaagaca
    24961 cgcatgtcca gacctagtga ttgcaattct ttcaccaata cgcggaatga ttccggtaca
    25021 cctggttttg gaattggttc acccttgacg attgcttcgt atgttttcac acgtccgact
    25081 acgtcatctg atttgtatgt caagatttct tgtaaagtat aagccgcacc gtaagcttcc
    25141 agtgcccata cttccatttc accaaaacgt tgtccaccaa attgagcttt acctcccaat
    25201 ggttgttgtg taaccaatga gtaaggtccg attgaacgtg catgcaattt gtcatcgacc
    25261 atgtgagcaa gtttgatcat gtacatcaca ccgacagaca cacgtccatc aaatggttca
    25321 cctgtacgtc cgtcgtacag aacagttttc gcatcgctgg ccataccggc ttcacgaact
    25381 gtttcccata cgtcttcatc acttgcacca tcaaatactg gcgttgctac gtggatacct
    25441 aactgacgtg cagccatacc caagtgtaat tccaatactt gcccgatgtt catacgtgaa
    25501 ggcaccccta aaggattcag catgatatcg attggtgttc catcaggtag gaatggcata
    25561 tcttcttccg gcataatacg ggatacaacc cctttatttc cgtgacgacc ggccatttta
    25621 tccccttcat ggattttacg tttttgaacg atataaacac gaactaacat gtttacgcct
    25681 ggtgacaatt catctccagc ttcacgagta aagattttca catcgtgaac gataccgccg
    25741 ccgccatgtg gtacacgtaa tgatgtatcg cggacttcac gagctttttc gccaaagatc
    25801 gcatgcaata gacgttcttc tgcagataat tctgttaccc cttttggcgt gactttccct
    25861 acaagcaaat cgccatcttg gacttctgca ccaatacgga tgatacccat ttcgtctaaa
    25921 tcttttaatg cgtcttcccc gacattaggg atttcgcgtg tgatttcttc aggtcctaat
    25981 tttgtatcgc gggcttctga ttcatattct tcaatatgga tagaagtgta tacgtcatct
    26041 tttaccaaac gacggctcat gatgatcgca tcttcatagt tgtacccttc ccaagtcatg
    26101 aaaccaacta aaacgttttg gcctagagcc atttcgcctt gttccataga tggtccgtct
    26161 gccagagtat cccctttttc gactttctca cccaaatgaa cgattggacg ttggttgtag
    26221 cttgttccgg agtttgaacg acggaatttt gttacatcgt atttatctaa tgcaccgttg
    26281 tcgcggcgta cgcggatttc agaagcatca acgtattcaa cgacaccgtc atgtttacat
    26341 aacaaagcag ctcctgagtc atgcgctgat ttgtattcca taccagtacc aacccatggt
    26401 gcttgaggat tgatcaacgg cactgcttga cgctgcatgt tggcacccat caaggcacgg
    26461 ttggagtcat cgttttccaa gaaaggaata catgctgtcg caacggcaac tacttgttta
    26521 ggtgatacgt ccatgtagtc tactttatca atagatactt ctaagttttc actttgagca
    26581 cgagccataa caacgtcttg agcaaatgtt ccatcttcat tcaatgggct gtttgcttgg
    26641 gctacgatgt aatggtcttc gatatcagct gtcaagtagt cgatttggtc agtaacacga
    26701 cctgtttcac ggtctacacg acgataaggt gtttcgataa atccgaattt atttactttc
    26761 gcataactgg acaaactatt gatcaacccg atgtttgggc cttcaggagt ttcgatcgga
    26821 cacatacggc catagtggga gtagtgaacg tcacgaactt cataaccggc gcggtcacga
    26881 gtcaatccgc caggtcctaa ggctgaaaga cgacgtttgt gtgtcaattc gccaagaggg
    26941 tttgtttgat ccatgaactg tgacaactga gaagaaccaa agaattcttt gatacttgcc
    27001 acaactggac ggatattgat caattgttga ggtgtcaatg tttcagtatc ttggatcgac
    27061 attctttcac gaaccacacg ttccatacgt gctagcccga tacggaattg gttttgtaaa
    27121 agttcaccga ctgaacggat acgacgattt cctaagtggt cgatatcatc gacattgccg
    27181 ataccttcca tcaagttcaa gaaatagctc attgaagcaa tgatgtcggc tggacgaacc
    27241 gtttttacag ctgcttctgg atagccgttt ccgatcacgt ttacttcacg ttcagggtct
    27301 cttggtgaaa atactttgat gacttggaca gtcattggat ctgtgaccac accgtcttct
    27361 gatggatagt aagtcactga atttaggcca ttatcaatga aaggagctag tgtttccatg
    27421 acttggtgag tcagaactgt tcctttttca acgatgatct cgcctgtttc tggatcaact
    27481 aatgtttcag ctaatgtcag attcaataga cgtgttttca aatctaattt tttgtttact
    27541 ttgtaacgac caacgtttgc taagtcgtaa cgttttggat cgaagaaacg agcattcagc
    27601 aagttacgtg agctgtctgc tgtttttggt tcgcctggac gcaaacgttc gtaaacgtct
    27661 ttcaaacctt cttctgttct tgagtcgctt gcgtttttat gcaagtcttt ttcgactgta
    27721 ttgcgtaatg tttcgctgtc gccgaagatt tcaaagatcg tatcatcgga accaaatcct
    27781 aacgcacgaa ctaatacggt taaaggaatc ttacgtgtac ggtcgatacg tacataagag
    27841 atgtcttttg catcagtttc catttctagc caagctccac ggttagggat aacagtagaa
    27901 ccgaatcctt cttttccgtt tttgtcgact tttccatgga aatagacacc tggtgaacgg
    27961 actaactgag aaacgattac tcgctctgcc ccgttgatga tgaatgttcc ttgttctgtc
    28021 attaatggga aatcaccgaa gaatacttct tgtgctttga tttcgccagt ctcgcggttt
    28081 gtcaaacgca atgttacatg taatggtgca gagtagtttg catcatgcgc acgagcttct
    28141 gctactgtat actttggttc tttcaattca taatctacaa attccaaaga tagattgcca
    28201 ttgaagtcat cgattggcaa aatgtcttca aacatttctc taagtccctc atctaagaac
    28261 cattggtaag agtctgtttg aatttcgatc aaatttggta attccaacac ttcactgatt
    28321 cttgcgaaac ttctacgttc gcgatgtttt ccgtatttta ctacgtgtcc agccaagctc
    28381 ttcacccctc taaaatgtca ttcaaaaaga ctacccgatc aattgttaca ataatatttc
    28441 aaatattatt tttaggtaac aacagacatt ttttgcggct tttaggcaaa aaaaaaccaa
    28501 aaatagaaca ttctcttgaa accttacttc gtgagcttct acttttgttt ccttatatta
    28561 aagccgaaac ggacaatatt tcgtttctgc cttttcttca tcagatagtt tttgcaactt
    28621 catatactac acttattttc tataaaagtc aacacttcct ggcgtttctt ctgctttttc
    28681 gcaacatttt cttaatgttt tcaattttat gttttcaatt atctgacaat tcttaattga
    28741 cagacctttt tatctcggtt atactttaga aaattcattt tgtggaggaa cttctatgcg
    28801 aaccaacatc aaccaaatcg caccaacaca actgaataac ttttacttta gctactttag
    28861 ataggtttgt attcatcatg atggatacaa acgttaaacg acagtttgta tctatgatgg
    28921 ctaaagaact ttgtattgca gcaaaaagcc acgtagacaa aatgctaagt ggctgagcag
    28981 tgaaaataca aagtaaaagt atcaagcatt ctactttttc aatttatttt ccgacttttc
    29041 aatccatttg gacattttgt ctgaatggat tttttgtttc ccaaaattaa ggaggaaaaa
    29101 gaaatgaaaa aaaatataat catcggatta ggcgctgccc tgctactagc tttaggaggt
    29161 tgcgcacaaa aagacaattc cgcagaggca tcagatggac aaaaaacaat tggcgtattg
    29221 caacttgtac aacataactc gttagatgct gcctataaag gattcaaaga aggattagca
    29281 gaagaagggt ataaagaagg cgataatcta acaatcgatt atcaaaatgc ccaaaacaat
    29341 caagacaacc tgaaaagtat gagtgagaaa ctcgtcaaag aagatcctga tttgctactt
    29401 ggaatcgcca ctccagcagc acaatcttta ttaaatgaaa caaccactat cccgatgact
    29461 gtcacagcgg tcacagactt ggaacaagcc catttagtca catccaatga aaaacctggc
    29521 ggcaatgtga ctggcacgac agatatggtg ccaatcgaaa aacaaatcgg cttactgtta
    29581 aatatcgtgc cagacgccaa aacaatcggc atcatgtata acaacggtga agcaaactct
    29641 aagatccaag ctgatttggc agaaaaagaa ttgaaaaaag cgggcgtcaa agtcaaagta
    29701 ctgacagcaa attccacaaa tgatgtccaa caagtgacga aaagtctagc taaagatgta
    29761 gatggaatct atattcctac tgataacacg ttcgcaagtg cggcaagtgt tgtcggtgaa
    29821 gtcgcaaaag aaacaaaaac acctgtcgtt gctggcgcag ttgatcaagt caaagacggc
    29881 ggtctagcaa cagtaggaat cgactacgaa agcttgggta aacaaacagg gatcatggca
    29941 gcaaaaatct tagacggcaa gaatccaagt gaacttcctg tcgaaacagc gaatgatttg
    30001 gaactgtatg tcaataaaga catggccaaa gcattaggca tcaatccaga cagcatcaaa
    30061 gaagctgaat aaaacacagg aggattctca tgacaaattt gctctctctt ttcttatcat
    30121 ctgcatcgca aggggtctta tgggccctac ttgcgattgg tgtatacgtc actttccgta
    30181 tcttggacat tgccgacttg actgctgaag gaagctttcc tttaggtgca gggatcgcgg
    30241 cagtaagtat tacaaatggc tatcatccat tgacagcttg tcttctaggc tttttagggg
    30301 gtgcggcagc agggcttgtt tccggactgc ttcacacaaa attgaaaatt cccgcccttc
    30361 ttgcagggat catcaccatg actggtttat attctgtcac atcccgtgtg atgggtgcgc
    30421 cgaacatctc tttattgggt cagccaacga tctttcattg ggtcgagtca ttcggcataa
    30481 caaaagacaa cgctgtactg atcgtcggtc tgctttttgc tttgatcgtt gtatgtttac
    30541 ttgttttgtt cttacgtaca gaaacaggac ttgctattcg tgcaactggc gataatctgg
    30601 caatgagcga agcaaacggg atcaacacag ataatatgaa gatcatcggt tatatgattt
    30661 ctaatggatg cattgctctt tctggctcct tgcttgcgca aaacaacggc tttgctgatt
    30721 taaattctgg tattggaacg atcgtaattg gacttgcttc tattatcatc gcagaagttt
    30781 tgttacgtaa ccagccacta tttctccgtc tcttgactgt cgttttaggt gcggtcattt
    30841 atcgttttat tttagcgtta gtcttcgaac tgaatgttga accaagtgat tcgaaacttg
    30901 cttctgcttt cgtgctagtg atctgtcttt ctttcccgca gatccagcaa aaattccatt
    30961 tacctcaaaa aaagaaaggc ggtagtgtca aatgaatgac gtcttaacat tggaaaatct
    31021 ccatcatacc tttgaaaaag gaacgatcaa cgaaaatcat gtcctgaaag gcattaatct
    31081 ttccttagat cccggcgact tcgtgacgat catcggtgga aatggggctg gtaaatctac
    31141 tttgctgaac agcatcgccg gtacattttc tgtcgatcag ggtcaaattt tattgaatgg
    31201 aaaaaatatc acgaaaaaat ctgttgtcga acgttcgaaa tccatcagtc gcgtattcca
    31261 agatcctaaa ctagggacag ccgttcgttt aacggtagaa gaaaatcttg ctcttgctat
    31321 gaaacgagga aaaaaacgcg gcttcttccg tggtgtcaaa ccacaagacc gaacattttt
    31381 caaagaccag ctggttcggc taaatttggg gttggaaaat cgtttatcct cagaaatcgg
    31441 tttgctgtct ggaggacaac gtcaagctat tacgttattg atggccacat tacagcgtcc
    31501 agaactgatc ttattagatg aacacactgc agcgcttgac cctgctactt ccaaaacggt
    31561 gatggaattg acagaaaagc tgatcactga gcagaatctc actgctttta tggtcactca
    31621 tgacatggaa gacgcgatcc gctatgggag tcgactgatc atgctgcatc aaggaaggat
    31681 cgtcgtagat atccagcaag aggaaaaaca gcagctcaca gtgccccaat tattagcgtt
    31741 gttcaaacaa aacagcggca cggcattaaa agacgatcaa gtacttttat cttcataatc
    31801 agaaagagga tgggacaaaa aatgaacgcc caccctcttc ttttttctct ttccttcttt
    31861 cgtttttgct ttatgtatca accgttgttt cttcgaaaac tattttgttc tctccttctt
    31921 cctgatagaa tggatgggaa aggagcttga tgccatgaca acgaaagaca aggtacttca
    31981 attattgaaa aagacaccag attttctttc cggagaaaag ttggcacagc aactggaagt
    32041 ttcccggaca agtatttgga aagccatcaa agaattggaa aaggcaggct atcgttttga
    32101 gcacgggcct acagggtatc gttacctgcc ctctgacgta ctagatgcaa aagaaatcca
    32161 gcaagagcta cggtcgttta ttcctgattt gaaagtacaa acgatgttga cttctgaatc
    32221 tacgatgaaa gatgccaaac tggcagcagc tcaaggagat tctgtttcga agttatttgt
    32281 tgctgataca caagaacaag ctcgtggacg ttttggacgg ccttttttcg ctgaacctgg
    32341 acgaggaatc tacatgagtc tcttgctcca tccagacaaa cggtttgatg aattgcctca
    32401 atatacgatc atcgcagcca gtgcctgtgt gaaagccatt gaatctgtaa ccggtcgttc
    32461 tgcttcgatc aaatgggtca atgatatcta catggatgga aaaaaagtct gtgggatttt
    32521 atctgaagca gtcagtgata tggaaagcgg caggatcagt catatcatca ttgggatcgg
    32581 gcttaatttt tctgttcctc aaaatcattt tccagctgat ctccaagaaa aagccatctc
    32641 tgtctttccc gacggacaag ctagtgtcac aaggaatcag ctaatcagca aaatctggca
    32701 agaattgttc acttatcttt ctcacccaga aaaagcgttg gacttctata aagaaaaatc
    32761 attcgtattg ggaaaaaccg tgtcgttcaa tcaaaaagga acgacttatg tcggtcttgc
    32821 aaaagaaatc ggtgatcatg gcgaactgat cgtccaaaca aatgaacaga agatgagttt
    32881 gtcttctggc gaaatcagtc tcacatctat ccgtacgcaa taattatcag atttcacaaa
    32941 aggacacctg atctcataca gtgtcctttt ttctgaattc tcttcaaaag actctcctta
    33001 ttctatcagc tgacagtttc tctatcctct agaaaaaggt attctaaaaa tatctataaa
    33061 gcatggtaca ctttctttaa atgaatcaat gcaaaaaaca agagagtcat cagtttacac
    33121 gatcaaaaga gatagcaggc cgcttccttt ttcaatctta tagatagacc atgggtaacg
    33181 ataaaaacaa aaaagaactt gcgacttatt ttgccgtaag ttctttttta gctagtgcct
    33241 taatctctta atcccaacaa acgtctcgca tagatcacct gaccagagtg catagcagca
    33301 tcatcaataa tagaaacgag acgcattcct cgtgtgactg gtggtgtcca gttctcgtca
    33361 attacttcat ctagctcgga aggttccagc cttcttaagt aagaaatagc aaaatcagca
    33421 gctcctcgca agtatcctag cagatcttct atttgattag cttggatctg ctttgcttgt
    33481 tccagactat gcttccaatc ttgttcgtta tctgctatcg tcatcggaaa ccggctttcc
    33541 cagtcttgac tatgccaaat tggagaagtt cctgctaaat cagcaatctg gaaatctaat
    33601 tctcgagcgg tatgccaagc cagccaagtc attgatttga tctgcggggc tctatcttcc
    33661 actggaaaac gattggcctc ctcaatagag acgccttcaa acaaacgttc aaaccgatcg
    33721 tatgcccgat ccacactctc gcatagcata tctaaataat ccatttattt tcctcctttt
    33781 tatttatcct acttactttt tcctaattct aggataccta cttatatgtt actacacacc
    33841 cttatttttt acactttaac agtcaccatc atcaatagtt tggagattga aagaaaaaga
    33901 tagtgtcgaa ctatcaattg aacaagaagc aacaatatga acgtttcatt aatattcttt
    33961 aaataaacaa tgatttttca aacgaacagt ttaagactag gagaaaaaga gtataatagg
    34021 aaaatgtttg aaaggaaagg aaggggaaaa atgaaaaaaa gggcaagttt actagtccta
    34081 gttgctttgt tgttcaatat cttttcgtat ggtgtggccg tttcagcgga aacatacatt
    34141 cctaaagacc ctgggaatat ttataatgat ctaactggta actatggctt gttaggaatt
    34201 gctagtcaat tccacgtatt tgctaaaaat aagacaactg tgaatgcaca tacagacgga
    34261 aatatagcaa cacatgaact tgatgcaaaa aataattttg gtacagagct tcatgaaggt
    34321 ttattgaacc aagaaataaa ttatatacaa aaaactgatt ctttcgctaa ttcttcaggc
    34381 atacctacag gtagcacaag aatgaataaa tttgtcgttg gcagtaacgt ggatgtagca
    34441 cttcaagatg gtcatcctac agtcaatgga tccaggcttg atcacttaac tccagatgat
    34501 ttttataaag atcgtgctgg gcatgaatat attgatttcg agcaggaatt tgctaaattg
    34561 aatgtagcag caaatgatct tgcaacgatc actccagcta aaacatatac tgctgcagat
    34621 tttccagaca tgaacaatcg gacaatcgat ttaactgatt tatcagatac cgggttttta
    34681 ttggtaaata tcgatgcaga agtgttaacg atgaatacgc cacttcaaat cattaatcca
    34741 aatgatcaag tagttgtatt taatgtcatc aacagttctt ctgcattgaa cgtacagtca
    34801 ccaatcaaat acaatgatcg ttcaaatcat gaaacagaag atttttcgga tgcgaatatc
    34861 tcttggaatt ttggaaatga gatgactgat ttgacaatca gtgctccttt tcaaggaaca
    34921 atccttgcgc ctaatgctac tatccgcatt actcaaaacc aagacggaac gattatagga
    34981 acaaatgtga ttttagatgc agctaccaat cgttgggatc ctaacgaaat atttatcagc
    35041 gacaatggta cagatacgac agacacaagc gacagtacag atacatctac cacaagtgat
    35101 agctcagatt catcaacttc aagcgacagt acagatacta cctctaccac aagtgatagc
    35161 acggatacat cagcttcaag cgacagtaca gatactacct ctaccacaag tgatagcacg
    35221 gacacatcag cttcaagtga cagtacagat actacctcta ccacaagtga cagcacggac
    35281 acgtcagctt caagcgacag tacagatact acctctacca caagtgatag cacggacaca
    35341 tcagcttcaa gtgacagtac agatactacc tctaccacaa gtgatagcac ggacacgtca
    35401 gcttcaagcg acagtacgga tacatctacc acaacaagtg atagcacgga cacgtcagct
    35461 tcaagtgaca gtacagatac tacctctacc acaagtgata gctcagattc atctaccact
    35521 acaagtgata gcacggacac gtcagcttca agtgacagta cagatacatc ctctaccaca
    35581 agtgatagct cagattcatc aacttcaagc gatagtacag acacgtcgtc taccacaagc
    35641 gatacacgtc atgcaagtgt ctccttctca aaaacaagtt cggataacag gacgaacttt
    35701 aacttaacta ataaaacaag caatgacaat ggaaacaaat caaaaagagc acttcctaaa
    35761 actggaagtc aatcgaataa ttggataaca ctcgcaggcg ttatattact tgtaatcggt
    35821 ttaagaatca cttttatgag ttataagaaa tcgaaaaggt aaaaaaatat tcaagataaa
    35881 aaagagcaac gtcgctaacg gacgttgctc tttatttttg ctttttatca taaatacaag
    35941 aactacaatt caatttgtca aaaaacacgt agatttcctt ctgagaaatt tatttacgta
    36001 tcgaaccttt ccctattcag tctttgcgat ccaaaatcag ttgggtaagg gaaaagagat
    36061 tctcttttgt tcgaagagag gtggcaggta atttctttat ttccttcaaa gccttttctg
    36121 tgtaagaggc cgctaacttc ctagcttctt ccacgcctcc tgtatgttca acgattttgc
    36181 gtacggcgtc gacatcttct tctgtcattg ccgctttctt ctctaaataa ggtagtagct
    36241 ttggtttgtt tttttgcaaa gagtagatca acggtaaaga atagacccct tgacgcatgt
    36301 cttctagtac aggtttgccg atttcctggc tctcttttgt ataatctaaa atatcatcga
    36361 taatctgaaa ggccatcccg atagaaagtc cgatatctcc tgcttttttt gcgaagcgct
    36421 cgcttgttcc actttcaaaa gcgccaacag aacaacttaa ttggaacagt tcagctgtct
    36481 tacctgaaat attttccaaa tattctttta cagaaagttc ataattatag cgatcatcca
    36541 tctgccctaa ttcgccattc aatacttttt ccatactgcg agaattcaat tggagacttt
    36601 tcaaggaagt ggagtaatca gacaatagtt taaaacagca gacaaataaa tagtctcctg
    36661 catagacaga aacgacatta ccaaactgac tacggatatt aggctgacca cgtcgcagat
    36721 ccgcttcatc aacgatatca tcatggatca gtgtggccgt atggagcatt tcgatagaag
    36781 ctgccaaagc gatggctttg tctcgatctt gctctggtcc aaattgtgag aacaatagct
    36841 gataggcagg acgcaacagc tttccgccgg catggatcgt ttctttgatt gcttgttcga
    36901 caggtttgtt tttcaggcgg acagtttctt ccatccgttt tagtgtttct gtcaattctt
    36961 tacttaaagc aggatagttt tcccacattg gatgtagctt cataaaaaaa ctccttgtgt
    37021 gacgattatt ttttggtaaa cattgggctc catttttgtt tggttccctg ctcataatct
    37081 ttttggaatc gttgaagtgt ctgcacatgt cgtaaaagat aattcgctgc gatcccgatt
    37141 gcaatccctg ataaaatccc taaaactgaa aggactggca agtacaacat gactgtccat
    37201 gattgtgcaa tgaaagaagc catcaaaagc tgtccgacat tatgcatgaa accgccggcc
    37261 gcactaattc cgatgatact gacacgtttt ggtcctaatt gcttgaccaa taacattcca
    37321 aaataactaa gcaaagcacc actcatacta taaaggaatg tggaaatcgt tccgcctagc
    37381 aaagtagtca ggaatagtcg tagccaaagt agtaagaagc tgtctcgttt tttcatggta
    37441 aaaatggcaa cgatcgtgat tagattggct aagcctagtt ttgcaccagg cgcaaaagaa
    37501 aaaggataag ggatcatatt ttccaataaa ccaatgatca ctccttgtgc aacaagtagc
    37561 gaaatataaa tcattttttg taatttactc atggtgtgtg aaggtctaat aaatcagact
    37621 gtcttcgtca cctccatctg ttgaacggac ttcgatcact aatttatgtg gcaaacagac
    37681 gatggtttcg ccatttttcg ttgcccagcc tcgacgtacg cagatctggt catcgcaatc
    37741 tgcttctttt atccgtactc gatcgccatc tatctcgatc aagttataat ctccgtgttc
    37801 atctttgtaa gtatatgtgt tgctctcttc tccagccatc aaagggaatg ttttgatttc
    37861 tgtgccatcc acacgcagta ccgcttcttt ttctgctgtt gcggtgtttt gctgaagacc
    37921 aaaaatcatg actggtaaaa aagaaagaac agtcaaaagc aaaataataa aaacatccca
    37981 aggtttgagc agactttttt cagcaaattc tttaaggctc atttttgttc tcctttttcc
    38041 agattaactc acccaatttt agcataaaaa caagaggttg tgacacctgt gtcttgcttt
    38101 gagtaataag aacgaagaat agaaaacagc tttccatgtt ttctaattct ttgggcttat
    38161 taccgagaag cagcaggtgg aacaccgttt ataacaagag gttgtgacag aagtgggcaa
    38221 cttcaagaaa taagaaggaa tttacgaaaa ttacttttca agtttttgtg aaatttcggc
    38281 ttatttttcg aagaagctac ttctggaaca ccgtttataa caagaggttg taacagaggt
    38341 tgtgacacct gtgtcttgct catggaggct tatctcgggc atcttattcc tttaatttta
    38401 cataaaaagg aggctgtgac ggtttgtctc agcccctttc tattgaaatc ttgcattcaa
    38461 ataacgttct ttatctattt atttcttgcc gtactgggaa cttggtctac cgtaccacca
    38521 attacctagt gtacgttgta gccatggaat tacccagcga tccaacccaa tcgcacgtcc
    38581 ggaaccgttc atcaatgcga aagcaacaaa aatgaaccaa atgtttaccc agtaaaacat
    38641 gcctgataag cagaacgtga tcaccaatac aatcgttgtc gcactgcata gccatgtgaa
    38701 taatccagca atcaatgcta atgccaaggc aatttcaaag aataccatga atttttggaa
    38761 gaacattgct acatcattgt ttggcatgat cacttgcatg atctttgtga accagtctgg
    38821 tgacttctca atcacagcca ttggttcttc accgtatgca taactcaaac caaaatgtgc
    38881 agcttgagtc gctgcttctc cggcacttgc ccctgctccg ctggcagctg tggttgcatc
    38941 accagcagca gaagcaccac tggcaacatc tgttacattg tattcccatg tccaagggaa
    39001 cacactgttt ccagtgaacc atgatccttc accaaaccaa gtacttggtt tgaacacttc
    39061 accattccca atgatttttt tggaagcttc aacgaaccaa actaaaccat agaaaatacg
    39121 aagtggcaca ctccataaga cattgccgta acgagaagaa tgtcctcgag tcacgttgcg
    39181 atcatctttg atatggaaaa actcatgcat taaatattgg aacatataat aaccagaacg
    39241 aatatcaaag aagtatttca aattcacgat atgtttcatt acgatagcta aaaatccgct
    39301 taaatggatc ttatcaaata ggttagctac cccccatttg gcaccaatag aaaccataaa
    39361 tccttgatag tttcctttaa atttatgctt ttctccgcct ttgatcgaag ccacgatatt
    39421 cgctgcggct gtgtgtcctg tttgttcagc agcttgaacg atctgcggtg tcggtgttcc
    39481 tgggaattct tcataaaaga ctaaatcacc aatgatatag atgtttttat cttcgtagcc
    39541 ttttgcctgc atatattcat tggcgatcaa ccgctgtccg cgtgctgatt ctaatccaaa
    39601 gtcagcagcg tcagaagttc ctttgacacc agctgtccaa atcagtgtgt gagtcggaat
    39661 gttgcgtccg tctttcaatt tgatatgatc agcagccact tctacgattg gtgcattcaa
    39721 taataattcc acgttgtgct tacgcaaata gcgttcagct tttgcagcat catttcgatc
    39781 caacatattc aagatcgtcg gcattgcctc tacgaccatc agtgtgattt cgtttggatc
    39841 taatttataa tctttggcta ggcgattttt ccagtcgatc agttccccaa tcatttcaat
    39901 accggtaaat ccagaaccac agacaacgaa agtcagcatg gctttacgca gttcatcatc
    39961 tggttccaat gcagcttttg ctactgtctt ctcaatatgt tcccgaatcc ttacagaatc
    40021 ttcaaatgac caaagggtga atccgtgtgc tttgacacca ggtgtaccaa agtcattcgg
    40081 ctctccgccc attccaagga tcagctgatc aaaaggatag gaacctaatt tggtcttgac
    40141 gatttttcgt tctttatcga taccgattac agtgtctgtg acaagttgta cattcttttg
    40201 acgagcaaac aaatgctgca gatcgtattg gattgcttct ggttctacac gtccgccagc
    40261 tacttcatgc aactcggtca tcatagtatg gtacgaatga cgatcgatca gagtaattgc
    40321 tacatcttct tttttcagtt tctttgccaa gtattttgta gctgaaacac cggcatatcc
    40381 tgctcctaca acgacaatat tttgctttgc cattgatcat ccgctcctta tagactatct
    40441 atataatgaa tatatgataa ttcttttagt ttgatactta ttacacaatc tccatatatt
    40501 atatagatgt tctgttcttt tgtctaactc cttttggatt tttttcaaat tttaaaataa
    40561 agcgtttttt cacgaaattt gctgaaagaa aacgtttgaa atattacatg ggttatttat
    40621 caaaaatagt tgacttatag gctaaaaaga ttagaatgta taatgaatca caaagaaaaa
    40681 ttgtggacta gaaatggaat ggtggggtat taaaatgaag aaaaaacgtt ttgtaactgg
    40741 atttgccgta ttagcatttt cagctctagt gttaggcgct tgcggcgcag atgacaacgg
    40801 aagctcaaac tcttcttctg aatcatctac agcacaatct tcgactgcaa aaagcacaac
    40861 agaatcatct gcaaaagttg tagctggcgg agatttgcaa gacggcacat ataaattaga
    40921 agaaaaaaat tattcaaatg gttaccgtgc agtctttgaa atggttgtaa aagacggcaa
    40981 aatcactgaa tcaaaatatg ataacgtcaa tgaaaacggc gaatcaaaaa caaaagatgc
    41041 agaatacaac aaaaacatgg aagcaaaatc tggcacaaac ccagaaaaat acattcctga
    41101 gttgaatgac caattcttaa aagcacaaag cgcaagcggt gtggaagtcg taactggagc
    41161 tactcactca tctgaaagct tccaaaacta cgcacaacaa ttgatccaag ctgctcaagc
    41221 tggtaacaca gatacaatcg aaatcgacaa tggtgctgat ttgaaagacg gcacttacaa
    41281 gcttgaagaa aagaactact ctaacggcta ccgtgtgcaa ttcgaaatga cagttgctgg
    41341 cggcaaagta actgaatcaa actttgatta tatcgacaaa gacggtaaat caaaacaaga
    41401 tgatacagaa tataatgaaa atatgaaagc aaaaagcggt acagaaccaa aaacttacat
    41461 ccctactctg aacgacgaat tcgtcaaagc aatgggcgaa gaagatggct cacctgctaa
    41521 tgtagaagtt gttacaggtg ctacacacag ctcacattca ttcatcatgt acgcacaaca
    41581 attagtgaat gcagctgaaa aaggcgacac tcaaacaatc gaagtagata atatcgtaac
    41641 agaaaaataa tcacataatc atactaataa aagttggagg gtgggacgat agccagatta
    41701 ggcagtcgtc tcacctttca aaatgaagag aggaaaaaga tgcggaaaaa actactattt
    41761 tccttaatca cactgggcat ctcttttatg gtgctaactg gctgcagcaa ctcagcaaat
    41821 aaaacaacca ctgaaagttc agcagctacc aagatattga aagatccata ttctgatgaa
    41881 cagttcttgt tagggactta cgttcgtatc cgtgtgtatg atgaaggcaa agaatcagca
    41941 ttgaaacctg ccttcgatcg agtaaaagaa cttggtgata aaatcacgat caatcaaaaa
    42001 ggttctgaga tcgatgaagt gaatgagcaa gctgggatca aaccagtaaa agtttctgat
    42061 gacgtctaca cgttagtcaa gcgggcctac gaatatagcc aagattctca aggcggattc
    42121 gatatggcga ttggtgcaat cacacaatta tggcgaatcg gttttgatga cgccagaaaa
    42181 ccttcccaag aagaaatcga tcaggcattg aaattagtgg attatcataa gatcgaacta
    42241 aatgacaaag aaaaaactgt ttatctaaaa gaaaaaggaa tgatcatcga tttaggtgca
    42301 atcgccaaag gttacattac ggacgaagta gtgaaagtct tgaaaaaaca aggagtgacc
    42361 actgccatcg ttgacttggg cggtaatgtc tatgtcttag gccacagccc aagaggcgaa
    42421 aaccaagact ggacagtcgg catccaagat ccgaacatgg ctcgcggatc tgttttaggt
    42481 tctattaaag aacggaacaa aacattagtt acctctggaa tttacgagcg ttatttagaa
    42541 gtagatggca aaaaatacca tcatttgttt gatccaaaaa cgggttatcc gttcgacaat
    42601 gatatcgcaa gtgtcacgat cataacagat aaatcaattg atggtgacgg gttgtctaca
    42661 gcagtctttt caatgggtgt gaaaaaagga ctggaatacg tcgaaagcga attgaataac
    42721 gggacggaag cgatcttcgt gacaaaagac gataaagtat atgtgaccga tccaatcaaa
    42781 gacaccttca aattatcgga ggactcccat tacacgatgg gtgaccgttc tgaactaaaa
    42841 taaggagtac gaaccatgac actaaaaatc tttttagaag tagttgaaat acgaacaaag
    42901 gtggctagtg tatttccttt tgcggtaggg gtattattct ctatcgcttt ttttcatgag
    42961 atacattggc taaacacttt tttatttttt attggaatgc tagtttttga tttagcgact
    43021 accgctatca ataattacat ggattatgaa aaggctcatt ctgaagtata taagtatgaa
    43081 gaaaatgtca ttggccgcag aaatctatct cctgtattga ttcgaaacat gattttagct
    43141 atgattgcat ttgtttttgt tatcggcgtt attctaacga tcaatacggg ttggctttta
    43201 cttttgatgg gatttatctg ttgttttatt ggagtattct atacattcgg accgattcct
    43261 ttatctcgaa tgcctttagg ggaaattttc agtggggtga ctatgggcct tggaattttt
    43321 gccatggtcg tttatgtcaa tacattcggt tcagatgtct atgatctgac agtcaacttt
    43381 tcatctggac aatttgcttt ggtaggaaat ctttgggcta tagcagcaat cgtattagct
    43441 tccttaccct tggtgtttac gattgccaat atcatgttgg ctaataattt gcgggatctt
    43501 gaccgtgata ttgaaaatca tcgctataca ctagtttttt atatcggacg tcctgcggga
    43561 acagtcttat tccagctatt gatgtatgct tgctatttcg tgattttaat ggggttgttg
    43621 agcggtatct atgaatggcc gatcttggtc accttcttga ccttgccaat tgtgaagaag
    43681 catttggatc aattcaagca aacgttaccc cagcctatca gtttcagcta ttccatcaaa
    43741 aatatggtct tattcaatgc cagttatgta ttggggctat tactaacaat cattatgcaa
    43801 gccatttaac agcatgaaga ggttgtgaaa aaactgccct gcatcaagta ataagaacag
    43861 ccaaataaaa atagctcctt atatttttcg tttggttggg cttattaccg cagatgcaag
    43921 cttttgaaca ccgtttatca ggaggttgtg aaaaagtcgc ttagtttcga gtattaagaa
    43981 aaattttgtg aaaatggctt tccacatttt tgcataaatt tggcttaata ccgagaaact
    44041 ggcttttgaa caccgtttat cagaggttgt gaaaaagtcg cttagtttcg agtattaaga
    44101 aaaattttgt gaaaatggct ttccacattt ttgcataaat ttggcttaat accgagaaac
    44161 tggcttttga acaccgttta tcagaggttg taaaatcagt gttttgtcct gagtattaga
    44221 gaaacaaatg gaaaaatagt tcctcatatt ttgccgtttt ttgagctaat accgaaggac
    44281 agctgattga acactatttg ttcggaaaaa gtgactaact atgacacaat ttttttcaca
    44341 gtcccttttt tcacttgatt tgattcgtac cagcgaactt gacgagaact ttcttggttt
    44401 ggtaactata tggtattctt ttaacatcct cgtttgcttc ttctaattta tttatacaaa
    44461 gtcacacttt tttaaacatc tgttcttcga tacatttcta tttcttttaa acatcgatac
    44521 caatattttg aacttaagag tgcataccaa atggattcat ttactcttcg tcagttacga
    44581 agataatcaa aatctccaac ttccctactt atttcgtttt gagaattatc gaagcaatct
    44641 tacataaata actaaaaaac aaaaaaccag aaaatcagta tactgatttt ctggttttac
    44701 atggtttgtt caataaaaag ctgttggaac tgttaatatt aaattatcgc atcgttacga
    44761 attcttccgc tcctgttgga tggatggcta ctgtattgtc aaagtcttct ttagttgcac
    44821 ccatcttgat agccacggca aaaccttgca gcatctcatc gacaccaaca ccaatagcat
    44881 gtaagccgac gattctttct tcttttccga cacagatcaa tttcatttcg catttttggc
    44941 gataatcatt taatgcaaag tacattgggg taaagcgtga acgataaact ttcaattcat
    45001 tttcgccata tttttccaat gcttcttctt ctgtcaaacc aattgttgca attggtggat
    45061 gcgtaaagac gactgtcgga acaagtttat agtcaagata agaatctttc tttccattaa
    45121 ataaacgttc tgataaacgt ctaccagctg cgattgcaac aggagtcaga tctaatttac
    45181 cgatcacatc accaacagca aaaatatgtt catccgttgt attttggtat ttatctactt
    45241 tgataaaccc ttttttgtcc aattccacat ttgtattttc cagacctaat ttttcggtat
    45301 tcggtacacg tccaccggca aaaagtacac aatcaccagt taaggtttca ccattttcaa
    45361 atagcaccgt atattcgtca ttgtttttag taatttcttt agcaatatga tgtgcgtaag
    45421 tttttattcc agcttcttgg tacatttgga tcaaattgtc cgaaagcatt ttatcaaacg
    45481 ttctaagtgg tcgttctttt cgatatgccc acatgacttc actacctaaa ccacaaaata
    45541 cacctgaaag ttcagccgca atgtatccag cacctaaaac gattgctctc ttaggtaact
    45601 ctgttaattc aaaaaatcca tttgaatcaa ttgcgtattc tccaccagga atggtcattt
    45661 tacttgcacg gcctccagta gcgatcaaga ttttaggagc acgataggtt tcaccgttta
    45721 cttcgatcgt ttgactatcg ataaaagtag cgtaaccttt tatagcctcg acatgattgc
    45781 tatctaatcc tctttgataa gcaccgtgta gaaaatcaat atatttttct cgtctttcta
    45841 ctagaccggc gaaatcaaca ttttttaaat ctgcttggat accgtaactt gaggcatctc
    45901 gttcaattgt ttccaaaatc gtacttgctt gccacataac ttttttaggt acacaaccga
    45961 cgttgacaca tgttccgccc aattcattcg cttcaatcag caaaactttt gcgccatgca
    46021 tccctgctct gtttgctgaa gcaataccgc cagagccgcc accaattacg atgtaatcat
    46081 atatttgcat tttgttcctc cttattgaag agatcaatca aaacctttat ttaacgatcc
    46141 tttttctaca gaaaacaagt gtaacacttt tcttgaattt tgtatgcgaa gatgcttaaa
    46201 acagggatat atcagctttt tccttattta cgttttcgga tcatatctcc aggtttcacc
    46261 ggctgtgcga ctggcatagt taaaatcatc attggatttg gtgctcggtc aatggattcg
    46321 ccttcttcat tatgcataac ttcaacggtt tgactgaaat gactgaatcc tggaccataa
    46381 aattcgattt catctcctac agaaaacaag tttctctgac gtatcgtagc aatctgtgtt
    46441 gcctcatcat aagataatac ttcaccgaca aatttatata ctgggatttt acgacgttca
    46501 ccgaataatt gttcgttttc agttggtgta ttgtagtaaa aacctgtagc taattctcgt
    46561 tgtgctactt tccataactc gtcgatccat tcttgcttgc agacatagtt ttcaggatct
    46621 tccatataac tgtctacagc cgctttatag acatttgaca ccgtagatac ataatggatc
    46681 gatttcattc ttccttcgat cttgaagctg tctacgccgt tttcaatcaa ttctggaata
    46741 tgttcgatca ttgacatatc cactgcactc atcgagaatt cttcttccac ttcgcctgta
    46801 tcagttaaac tgtttcgttc ttgaccaaaa ggcatatcga acaatccata cttccagcga
    46861 caagattgag aacatccgcc acggtttgca tcccgcatcg acatatggtt ggacaatgtg
    46921 catcgaccag aataagagat acacatggca ccatggataa atgcttcgat ttcggtatcc
    46981 gtatttttac gaatctctgc cacttcctcc attgaaactt cacgagctaa aacgacacgt
    47041 tccaaccctt cttctttcca gaattccaat gtctcaaaat tagttgctga agcttgagtg
    47101 gataaatgga tcggcagacc tggggcttct gtcgcacaaa tttcgatcaa agctggatca
    47161 gaaacaatca ctgcagaaat accgacatca cgcaaactgc ggaaaaattc ccctgctcca
    47221 tcttcatttc cttcatgggt gaccatatta gcagcaacgt ataccttcgc atcatgttct
    47281 tttgcaaaag caactccttc agccatttct tcataagtaa aattacctgc acggcttctt
    47341 aaaccatatg cattcccgcc aatatatact gcatctgctc cataatggat cgcggttttt
    47401 aacttttcca gtgttcctgc tggcgctagg atttctggtc gtttcaattt tctttttact
    47461 gtcatttctt tcatcatact ccttatttga tagcagaagg atcaatataa aagaatcctg
    47521 tatctaattc acggttttca ggatgcaatt tactgatttc gctagtcatt ttttccgctt
    47581 tttcttgatc ccagctacct tctaaaattt gtttttttgc ttcggcgaat agtcgagtga
    47641 tgtttacgaa tttttcgcct ggagtaaata atccgtccaa tttccatgta cagtaatgat
    47701 gctgataaag ttcattcaat tcattcgaga gattcagatc attgctggca aaaatatgtg
    47761 tgccatgact atcttcataa atagaataat gtgtctcttc ttttttaggt tccgagataa
    47821 aaagacctct ttcacgatct ttttgttcgt cttgtttcgt atagttatag taattctgta
    47881 gcagtggacg tttggattga tggatgcatg tagcaccata cacaagggtt tctacaggaa
    47941 tatcgagttt ttcttccatc gccttcatct cttcaaaagg aacttcacga gcaagtactg
    48001 ctccaactgc gccttttttc gcccagaaat tgatttgacg tgcgctcgtt actaatgttt
    48061 cagcatcata aatatacggt atacgcaatt caggattttt tttcatcgta taaataatac
    48121 ctgtatcacc aactgtgatc acatctacgt ttatttcttt taaaaaagcc aaatattcag
    48181 gtacattttt cattttttca ggatgaatga tcccatttac agcaatattc gcttttttac
    48241 catacttgtg tgctaattct accaattgtc gctgttcttc tcgtgtaaat gaagcaggca
    48301 gacgcaatcc aaattgctct tcaccgaaat atagtgtatc tgcacctgca gcaagcaact
    48361 gttctgcttg ttcaatagtt tctactgtgg ttatgagttc gatcataatt ttctcctcct
    48421 acaacttcat aatagtagca tagctctaaa aactttccaa tacttcttct atttaaagtc
    48481 ggatttttga aaattatttc acaaaaaatc aaaataaagt tcgatatatt ctgtccaaat
    48541 ataaaaagag attgtgacaa aaatcttgtc acaacctctt aatcaagtct tactattata
    48601 aaaattcagc tacacaggcc aagtaactat agggaaccta ctgtttcctt tttaggtccg
    48661 cgaacattca atgtgatctt accttttgaa gcacctactg ttacgtggtc acctaaacga
    48721 atttctccgc caagcaaagc ctcgctcaat cgatcttcaa tttctttttg aagtgcgcga
    48781 cggattggac gtgcaccata ttcaggatca aatccagctt tcccgatcac atcgattgca
    48841 gatggtgtga ttttcacttg aatatcttgt tctttcaatc gtttgataat cgctttgctc
    48901 atgatttgga cgattgtatg gatttcatct ttactcaatg aatggaagac gacagtctca
    48961 tcgattcgat tcaagaattc aggtcggaaa gctttcttta actcttctaa gatacgtttt
    49021 tgcatggctt tgtgatcttt agtaacatct tggacattga atccgacatt tttttcttct
    49081 cgaatttgtg tcgctccgat atttgaagtc ataatcatga ttgtatttct aaagtccact
    49141 tttctacctt tagaatctgt taaatgacca tcatccaata cttgcagaag taaattgaaa
    49201 acgtctggat gtgctttctc cacttcatct aagagaatca ctgaatatgg tttttgacga
    49261 attttttctg tcaactgtcc accttcatca taccctacgt atcctggagg agacccgatt
    49321 aatcggcttg tactgtattt ttccataaat tctgacatat caacacggat cagggcatct
    49381 tcacttccaa acatgacttc tgaaagtgct ttggccaatt ctgtttttcc gacaccagtt
    49441 ggtccaagga acatgaatga accaattgga cgatcaggat ctttcaatcc gcttcgtgca
    49501 cggcggatcg cacgtgaaac tgctttgaca gcctcatctt gaccaacgac acgttcatgc
    49561 agaagtcctt ccagttctaa caagcgttcg ctttcttttt tctccaactg ctgtagaggg
    49621 acaccagtcc actccgatac aactgtagca acatcttctt ctgttacacg atcagaataa
    49681 ccttgctcct gttttgcttc aattgcagaa actttttgca gctttttacg tagtgctttt
    49741 tcttgtctgc gtaattgagc agcattttca aagtcttgtt tttggatcgc tgcttccttt
    49801 tcttgtacta gttcatcgat ttctagtttg atcacagttg attttgttaa gtgatcggtt
    49861 tgatccaaac ggactttcgc tgctgattca tcgatcaaat caattgcttt atcgggtagt
    49921 tgacgagaag tgatataacg aacagaaaga ttgactgctg ctcgtacagc ttcatctgtg
    49981 atctccactc catggtgctc ttcatatctt gtacgtaatc cttgaagaat cacttctgct
    50041 tcttcaggag ttggctcatc tacttgaatg cgagcaaaac gtcgctctaa tgcagaatct
    50101 ttttcgatat atttttgata ttcatctagg gtcgttgctc cgatcgtttg taattctcct
    50161 cgagctaacg ccggtttcaa gatattcgaa gcatcaattg ctccttctgc tccacctgca
    50221 ccaattaagg tatgcaattc atcaatgaat aagattactt gaccatcatt atagatttcg
    50281 tcgatgacct ttttcattcg atcttcaaat tcaccgcgat atttcgtacc agcaaccaaa
    50341 gatcccatat caagcatcat caggcgcttt tcttgcatgt cttctggcac ttcacctaaa
    50401 atgatttttt gagctaaccc ttcagcaatg gctgttttgc ctacaccagg ttcacccacc
    50461 agtacagggt tattctttgt ccgtcggcta agaatctgga taagccgttt cacttctctc
    50521 gaacgaccaa caactgggtc cagcttgttt tcttttgcta atttcgtcaa atcacgtgct
    50581 aatgaatcta gcgtaggtgt tccttgcggt gtttgccgtt tatcttgtac atttacattt
    50641 ctacgacgat tcattccttt tccagcagaa tcaccagcta gccccatttt ttgtttcaga
    50701 acttgacggg cttttgaaag gctgatacct aagttgatca tgattcgagt agccatgata
    50761 ctttcatctt ttaataagct catcaaaata tgttctgttc caactttttg agaaccagtt
    50821 ttcttcactt cgctgctagc taaagcaaaa acttgtttca ttcttggtga atatggaaga
    50881 aagactcctt tagggtaaga acggatacca ccataaccaa tcagatgttc aatttcttca
    50941 taaatttccg tttcattcaa atccatttca cgtaatactt ttccagcaat tccattttgc
    51001 tcgaccacta aggctagtaa aacatgttct gaactaactg attgatgttt caaccgtttt
    51061 gcttcttctt gtgcaatttc taatactgct tgggctcttt cagtaaaaag ttcttccatc
    51121 taatctcact tcccatcttc ataacttaaa cgttccagaa atgctcggag aatccgagca
    51181 cgtgtatgat cttcatattc attgaaattc aatgtgtttt ttgcaattgc actcaacatc
    51241 aagtttcctt ctttttttgt aattatttgg tcttcataaa gtttttgaat aatagcaaaa
    51301 gcatttttct cactgattgt atcatcaaat aactgattta tctgctcaag taattgtttt
    51361 ttatctgaaa tccttacttt tgcaattcgt atataaccac caccaccacg tttgctttct
    51421 actagataac cacgttggat ggtaaaacgg gtattaatca catagttgat ttgtgaagga
    51481 acacagttaa agaggtctgc catttcaatc cttcgaatct cgatcatttc actattttcc
    51541 agaattttct ttatatatga ttcaattaaa tccgaggtat tctgatgtgc catcttattc
    51601 attccctttg actaatattg accttaatta taacgaattt gataatagaa agaaagaaga
    51661 acgctttttg ttaaataaat cctttagaat caacaaaaaa aatacggaat atactattcc
    51721 gtatcgacgc gcccaagagg agtcgaaccc ctaacctttt gatccgtagt caaacactct
    51781 atccagttga gctatgggcg catattcaat ttaaaaaaat tataaatcat ttgaaaacaa
    51841 aaagcaagat aaaaaaaatc gggaagacag gattcgaacc tgcgacccct tggtcccaaa
    51901 ccaagtgctc taccaagctg agctacttcc cgtttaaaaa ataaaaaaga c
//
LOCUS       DACDIE010000010        51195 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.010, whole genome shotgun sequence.
ACCESSION   DACDIE010000010 DACDIE010000000
VERSION     DACDIE010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.010"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            290..1135
                     /locus_tag="IV250_000708"
     CDS             290..1135
                     /locus_tag="IV250_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8768012.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1416..2636
                     /locus_tag="IV250_000709"
     CDS             1416..2636
                     /locus_tag="IV250_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HAP8768013.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2633..5353
                     /locus_tag="IV250_000710"
     CDS             2633..5353
                     /locus_tag="IV250_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8768014.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5337..6281
                     /locus_tag="IV250_000711"
     CDS             5337..6281
                     /locus_tag="IV250_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8768015.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6350..7360)
                     /locus_tag="IV250_000712"
     CDS             complement(6350..7360)
                     /locus_tag="IV250_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP8768016.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7540..7884)
                     /locus_tag="IV250_000713"
     CDS             complement(7540..7884)
                     /locus_tag="IV250_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP8768017.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7877..8311)
                     /locus_tag="IV250_000714"
     CDS             complement(7877..8311)
                     /locus_tag="IV250_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HAP8768018.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8449..9186
                     /locus_tag="IV250_000715"
     CDS             8449..9186
                     /locus_tag="IV250_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8768019.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            9179..10390
                     /locus_tag="IV250_000716"
     CDS             9179..10390
                     /locus_tag="IV250_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8768020.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10597..11376
                     /locus_tag="IV250_000717"
     CDS             10597..11376
                     /locus_tag="IV250_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAP8768021.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHGD
                     VNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11446..12108
                     /gene="trmB"
                     /locus_tag="IV250_000718"
     CDS             11446..12108
                     /gene="trmB"
                     /locus_tag="IV250_000718"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAP8768022.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12206..13960)
                     /gene="ade"
                     /locus_tag="IV250_000719"
     CDS             complement(12206..13960)
                     /gene="ade"
                     /locus_tag="IV250_000719"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAP8768023.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            14092..15021
                     /gene="cpaF"
                     /locus_tag="IV250_000720"
     CDS             14092..15021
                     /gene="cpaF"
                     /locus_tag="IV250_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAP8768024.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            14978..16027
                     /locus_tag="IV250_000721"
     CDS             14978..16027
                     /locus_tag="IV250_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HAP8768025.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            16024..16302
                     /locus_tag="IV250_000722"
     CDS             16024..16302
                     /locus_tag="IV250_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAP8768026.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16277..16747
                     /locus_tag="IV250_000723"
     CDS             16277..16747
                     /locus_tag="IV250_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAP8768027.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16764..17066
                     /locus_tag="IV250_000724"
     CDS             16764..17066
                     /locus_tag="IV250_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8768028.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            17083..17454
                     /locus_tag="IV250_000725"
     CDS             17083..17454
                     /locus_tag="IV250_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HAP8768029.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            17426..17785
                     /locus_tag="IV250_000726"
     CDS             17426..17785
                     /locus_tag="IV250_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8768030.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17957..18964
                     /locus_tag="IV250_000727"
     CDS             17957..18964
                     /locus_tag="IV250_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8768031.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            18989..20173
                     /locus_tag="IV250_000728"
     CDS             18989..20173
                     /locus_tag="IV250_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAP8768032.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20610..22193)
                     /locus_tag="IV250_000729"
     CDS             complement(20610..22193)
                     /locus_tag="IV250_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP8768033.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22771..22914)
                     /locus_tag="IV250_000730"
     CDS             complement(22771..22914)
                     /locus_tag="IV250_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAP8768034.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(23136..23615)
                     /locus_tag="IV250_000731"
     CDS             complement(23136..23615)
                     /locus_tag="IV250_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP8768035.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23688..23972)
                     /locus_tag="IV250_000732"
     CDS             complement(23688..23972)
                     /locus_tag="IV250_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8768036.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(24050..24244)
                     /gene="ssrS"
                     /locus_tag="IV250_000733"
     ncRNA           complement(24050..24244)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="IV250_000733"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24316..25602)
                     /locus_tag="IV250_000734"
     CDS             complement(24316..25602)
                     /locus_tag="IV250_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAP8768037.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25740..26411
                     /locus_tag="IV250_000735"
     CDS             25740..26411
                     /locus_tag="IV250_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAP8768038.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26377..26865
                     /locus_tag="IV250_000736"
     CDS             26377..26865
                     /locus_tag="IV250_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HAP8768039.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26878..27825
                     /gene="prmA"
                     /locus_tag="IV250_000737"
     CDS             26878..27825
                     /gene="prmA"
                     /locus_tag="IV250_000737"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAP8768040.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27826..28590
                     /locus_tag="IV250_000738"
     CDS             27826..28590
                     /locus_tag="IV250_000738"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAP8768041.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28823..31036
                     /locus_tag="IV250_000739"
     CDS             28823..31036
                     /locus_tag="IV250_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAP8768042.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            31047..31493
                     /locus_tag="IV250_000740"
     CDS             31047..31493
                     /locus_tag="IV250_000740"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAP8768043.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31664..32923
                     /locus_tag="IV250_000741"
     CDS             31664..32923
                     /locus_tag="IV250_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HAP8768044.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(33012..33959)
                     /locus_tag="IV250_000742"
     CDS             complement(33012..33959)
                     /locus_tag="IV250_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP8768045.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34353..35645
                     /locus_tag="IV250_000743"
     CDS             34353..35645
                     /locus_tag="IV250_000743"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAP8768046.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35660..37429
                     /gene="aspS"
                     /locus_tag="IV250_000744"
     CDS             35660..37429
                     /gene="aspS"
                     /locus_tag="IV250_000744"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAP8768047.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37538..38413
                     /locus_tag="IV250_000745"
     CDS             37538..38413
                     /locus_tag="IV250_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8768048.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38679..40562
                     /locus_tag="IV250_000746"
     CDS             38679..40562
                     /locus_tag="IV250_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP8768049.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40637..40921)
                     /locus_tag="IV250_000747"
     CDS             complement(40637..40921)
                     /locus_tag="IV250_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP8768050.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40911..41645)
                     /locus_tag="IV250_000748"
     CDS             complement(40911..41645)
                     /locus_tag="IV250_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAP8768051.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41774..42397
                     /locus_tag="IV250_000749"
     CDS             41774..42397
                     /locus_tag="IV250_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAP8768052.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42589..43710
                     /locus_tag="IV250_000750"
     CDS             42589..43710
                     /locus_tag="IV250_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAP8768053.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43717..46845
                     /locus_tag="IV250_000751"
     CDS             43717..46845
                     /locus_tag="IV250_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HAP8768054.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46864..50058
                     /locus_tag="IV250_000752"
     CDS             46864..50058
                     /locus_tag="IV250_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP8768055.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(50204..50911)
                     /locus_tag="IV250_000753"
     CDS             complement(50204..50911)
                     /locus_tag="IV250_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP8768056.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        16751 a         8945 c        10631 g        14868 t
ORIGIN      
        1 tttaaatctt ttgctaagtt gaactaatag agtcttttat gaatatcgaa gttagttttt
       61 cacctaactt cgttttttat agttggttat tataaaatag taatatagct gatttggatt
      121 caattcatat ttatttataa atcaaaaata cagagaatag tatataaatg tcctttccta
      181 ttcttaaaat gatgtttgta cttacttaaa ttaggtataa ttagtatatg ttttgtgaga
      241 aatttagact tacaataagc agtgaattta taatcaacgt ataggagaca tggatgaaat
      301 gaaactaatt ggtatagatt tagacggtac tcttttgaat tctgaccaga gcattagtca
      361 aaaaaatgca agaacaatga aacatttacc ggaagactgt tttccattca tttgttctgg
      421 aagagaagta gaagacatta ataatattct caaaaaatct ggtttaagga taccagcagt
      481 agggttaaat ggttctgttg gatttgatgg agaaaaaaag atatttgagt cttccttcga
      541 taaaaatgat gtaaaaaaaa ttgattctgt cctatcgaaa tttccaacca aaatatatac
      601 aaatctcgga agttatgaat ctcagaatta taaaaataaa atgaaaaaaa tttttcacga
      661 ggtagggagt gaattttcta tcaaagagct aaactatgag cttgagtatg aaaaatctat
      721 ttgttcaatc ccttataata atattgagga aattatagaa aaggatgcta tcagcattta
      781 caaatttttt atttttattc ctaatcgtca attgaaagct aatatattta accatttgaa
      841 aaagatgcta aatgtgtctg ttactgaatc ggcacctgta aatattgaaa tcgtacctca
      901 aaatgtatct aaaggtgtgg tgtttgatcg tttggaatca atctacaatt taaagaaacc
      961 tttgagaatc gcgatcggag atagtttaaa tgacttatct atgtttgaaa gtgcagatat
     1021 tagttttgca atgggaaata gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt
     1081 ttcaaatgat aaagacggag tatctcaagc atttgaaaga attcttacca tataaactaa
     1141 ttaaggtagt atagaacata gaattctaaa tacggaaaaa tggagcataa attccagtac
     1201 aaatagtact tcttcagtgt caaacgaagc gaatataaca tagatattta ggattgtgac
     1261 aaaaatcatg tcacaatctt tttttcgaat aaacttattt tttgaagtcg gccatttatc
     1321 gtaatttctt tgcataatac gaacaaattt tcggttattg atgaaaaaaa acattgaaaa
     1381 tggtatgata aaagaaaaaa aggaggaaat ttttaatggt gaaatttatc catgcggcag
     1441 atttacatat ggatcggtca tttgaaggac tgacaactct tgataaaact gtacaggaaa
     1501 aactattgaa gacgaatctg accgttttat caaatatcat tgaacaggca ataataaata
     1561 acgttgattt tgttttactt gcaggagata attttcatca aaatcgtcct tcattaaaaa
     1621 tacagaaaca tttttcagaa cagatgaaac ggttagaaaa gaaccatata cctgtattta
     1681 taatattcgg aaatcatgat ttctatcaaa aagagcggta ttggttctcc tttcctaaaa
     1741 atgtccattt atttacatcc gaaacggtag agacaaaaaa aataacgata aaaagtggag
     1801 aaactgtctc tctcagtgga tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga
     1861 tggaatttcc ttctagagaa ctcacagatt accatattgg gttgtaccac ggggaacctg
     1921 gcgtctctca aaaaggaaac tatgctcctt tccaaccctc aaagatgcaa gaaaagggat
     1981 acgattattg ggctttaggg catatccatg tgccaacggt cttaaatgaa aagcaaacaa
     2041 ttgtttatcc aggtgcgcct caaggtcata ctaaaaagga acaagcttct acatctattc
     2101 tactagttga attaagtaaa ggaagttgcc aaatacatcc gattaaagta gcagaggtct
     2161 attggaagac aaaggaaata tcattgagga aagcaagaac gacaaaagag gtcattgttc
     2221 acattcgtca gcagctttca aaagtagagg atggatttag tttaattgag ataaaattaa
     2281 aagattatga ccatctcaat acagatgttt tggaacggat acaatcagga gagctattag
     2341 actatttgtt agaggaattc tcagatagga taaatgattt ctttatttgg cgtatttctc
     2401 tcatagagaa tactttttat acgaagaccc cacttgctgc ttctgctaaa ttgatggaac
     2461 agttatttca atactatcag acacctcaag atttccaaca aattttaaat gaagtatata
     2521 gtcatcaaga agcggctaga atattgagcg aactgccgga atatcaagaa gaaacacttg
     2581 aaaaagccaa gcagctgctt aatcaagatt ttttatttga ggaggatcag gaatgaagat
     2641 tttaaggatc gagattgctg cttttggaaa atggcgtcaa aaaagttttg acttttattc
     2701 aggcaatcag ctgatctatg ggggaaacga agcaggaaaa tcaacgattt atcaatttat
     2761 tcaagcaata ttatttggtt tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa
     2821 agacggctca gcctatggcg gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc
     2881 tgtcgagcgc tacaagcagc aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt
     2941 gggaagtgat gagttactag aaaaactgat cgcacctttg acgaaagagt tattccagca
     3001 ggtatttact ttccagcaag agcagttgat ggaactagat catttgagag aaaaagagct
     3061 acatgatgcg ctgatttctt taggcatcac gggcagttct caagtattcc aaaagcggca
     3121 agaatattat cagaaagcac aacaattatt caagaaaaaa ggccaaaaat tactaatcaa
     3181 tcaaaaatta aatgaatgga aagagctgca agaaaaaatt catcaaaaac aagaacagga
     3241 agcttatttt ggcaagttga tccaacaaga acaggaatac gagtcgcaac agcagcagtt
     3301 attggcacaa ttaaaagagg cgaacaaaca attttcatta gccagacagc aagaacttaa
     3361 tttttcttta tatgaagaat ggcaatctct caaaaaaaac agcactacag gtcttcctga
     3421 tgagcaactt cttttagatc tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg
     3481 tttggaagaa ttagaagcaa cactggaaaa gcatagcggg atggatcagc agtcagccag
     3541 atattatttt tatttagagc aggagcagga actaaaagat ctccagcaac ttcaatttac
     3601 agcggaacaa ttaaacgaag aatggcaaag attacaaatt gagactagag aaacaaattc
     3661 agaattgaag attttggaac aaaggtggca ttggcagaaa aatccgttac cggaagaatt
     3721 tttatatgaa gaagaatgga agcatcttct ggctgaagaa agaaggattg ctgatctaga
     3781 aagccagatt tctgtgcgtc tccaaatagc taaagaacag caagagatga ttgaagcaga
     3841 agtgaatgac ttggaagaga cctatccagc attattacaa aatcagtcag agatagcacc
     3901 agtctcttca tctaaaccgt atatgctgat ggctggcgga gcaataatca ttgcaggtat
     3961 atttgtacca atgcccttga aagtgatcct tttactaatt ggtgcaggca tgattggcta
     4021 tttcttctac aaaaggcaac caaaaattaa aactaaaaat gatttctctg agataaaagg
     4081 aatgtggcaa gaaaaactag gacaacttga tctagcctta gctcaattag ctgaagcaga
     4141 agaaaaacaa aagagaactg gagaagaaaa acagattctt cagcaagaag tatatcgttt
     4201 tgcggatcat cggcacttag gaaagatgga tacgctcaaa ctcatgaaag aatatggaca
     4261 ggaagtcata gattatcaga aattatatgg cgtatttgag cggaaacata aaagagaaca
     4321 ggaaatacaa gaaaaactac gacaaatcga ccaaaaattt gagtttgtta aagaatggtt
     4381 accgatacaa gataagacaa tttcagaaaa aatggtattg ctttcccgtt ttatacaaga
     4441 gatggaacag atgaaatttg ccagaagcta tcagcaaaat actttattga agcaagaaat
     4501 caatcaattg aaaaagaaac aaaaagtatg gatacaagaa tacagcgaac aattgactca
     4561 agcaggctta tcttatcctt cggaagtacc tgtcttttta caaaaagcca aacaagccga
     4621 acagcagatg aaaagataca atgaattatc ggaaatgctt actccactat ttcctgaaga
     4681 gctgacaaaa gagaaattat ctgtaagact gcgccaactg aaagaaaagc aatcccaact
     4741 ccagcaagaa gaaaaagaag caagtgaaaa tcggcaacga ctccaacttc aagtagaaga
     4801 gttgcaaaga gacggcacat tggacgaact ttatcaacaa gctagccggc aaaaagcaaa
     4861 attacaggaa ttactggagg aatggggagc acttgaaatc gccgggacat tcttaggtga
     4921 tctttcaaca gagatgagtg aaagacaatt accccagttg ttaagagcag caggtgatta
     4981 tttaggtatt ttaacaaaag gaaattatca gaagatatta ctgaaaaatg atacactgac
     5041 tttaacagac ggatatacag aatttcccat ctatgagtta tctacaggta cgaaagatca
     5101 gctgattatg gcgatgagat ttgcttacct ctcattagaa gctaaccgtt cgatatgccc
     5161 gatcatcatt gatgatggct ggcttcatta tgatcatcag cgaaagtatc aattagctga
     5221 acttttagct aaatttggag aaaagtatca aataatttgt ttttcttctg ataaagaaat
     5281 ggtaagctat taccatgaga tgaaccaaat cgtaaaacaa ttagaaggag caaataatga
     5341 aaaaaattcg tgatttagta atcgatgagc agtttgaaca atttgtcctg atcaaaagtg
     5401 cagatgtaag aatcgctaaa aacggaaaaa aatttattgc ttttactttc caagatactt
     5461 caggtactat cgatggtaaa tactgggatg cttcggagga ggaaatcaat aaatttacag
     5521 ccggcacagt cgtttttcta aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa
     5581 agattcttca tatgcgctta acaagggtag atgagccagg agagcctagt ttatacatgg
     5641 aaagagcccc tttgaagaaa gaagaaatgg aagaagaaat caatcaattg ttgtttgaga
     5701 tcaccaatgc gcattggaat cgtattgttc gctatctact taataaatat caaaaacaat
     5761 tttttgagtt tccggcagca aaaagaaacc atcatgcatt tgccagtgga ttagcttatc
     5821 atactttgac aatgcttcac ttagggaaat caatcgccga tgaatatcct gagctgaatc
     5881 gagcattgtt gtatgctggt atcattttgc atgatttagg aaaggtgatt gaactttccg
     5941 gtcctatgtc gacagaatat acgcttgcag gaaatttgat cggtcatctg gtattgattg
     6001 acgaagaaat aactaaagcc tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc
     6061 ttgtattacg tcatatggtt ttagcacacc atggcttatt agaatatggc tcacctgtac
     6121 gtccaagaat aatggaagca gaaatattac atcaaatcga caatatcgat gcttcgatgc
     6181 agatgatgtt gacttcgatt cgtcaaacag aaccaggaaa atacaccgat cggattttcg
     6241 gtatggacaa ccgaagtttc tatgtaccaa aagatttata gcaaaaattt atttttttca
     6301 ttcaaaacaa aaaaagcgag acataagtct tcgctttttt tgttttcttt tattttgatg
     6361 aatcagttgt ttcttcagtt gaagattctt ttgttgaatc agttgatttt gtgtcagaac
     6421 tctttgttga agctgtagtt tcacttgagt ctttcgtact tgatgaagtt gttgtgaatg
     6481 cactcaacac attttcaaag gcatcatctt tgatttttac atttgcatct tttaattctt
     6541 caccgattac tttagttgtg aatgcattgt cacttagttt agtttcagta gcgatatctt
     6601 ttagttgatc tttgtattta tccatgtcat tgcctttatt ttggtttttc accattttga
     6661 caacgtagta ttctgtcgca taagaagtag gatttgttgt agtaataaca tcggagatct
     6721 caccgtcttt caatttgaaa gctgcttctt ttacttctgc aggaatagtt gttgtcgttg
     6781 agtcgaattt aactttaccg ccatcttctt ttgtctctgt atcagttgat ttgtctttgg
     6841 ctaatttaga gaaatcatct ccatcatccg ctgatttttt cacatctttt gcttcatctt
     6901 cacttgataa tttgatgatt tgtgcttcta cttctggatg gaaagatttc caagcagttt
     6961 tcaaatcgtc atctgtaata tctacgtgag ctttcaagcc agcttcaaaa gctaaattgt
     7021 tacgaatata ttctttataa gtatctttcg tatatccagc agcttctaat tgtgattcaa
     7081 aagtatcacc taatgatttc gcttgtttgt cgtattctgc atccacttgc ttatcggtta
     7141 ctttatcgcc atatttgtta ttgaatactt tataaatgat catccgttgt actaaggatt
     7201 ggttcgatga ttcaagtttt gcttcatcat agaaatcaga tacagtgatc gttccgcctt
     7261 tcatcgttgc gatatcttta ttagtatcgc ctgaacaagc tgctagagtt aaaacagcca
     7321 acgcactagt agctgccaaa ataattgatt tttttttcat taaaatttgc actcctgtaa
     7381 ttagttatta tttttatata ttaactatac catatcccat ttcctttaaa aacgttgtcg
     7441 tggtagtttc aaacaaaatt caaaatttca tcacattcct aggaaatacg caaacttttc
     7501 tcacttatat agttaaatct gttgagaaaa taatcattat tatatttgtt caggatactc
     7561 tggcagatca gcttttagtt tttctacttg ttcattgatt tgtgtgatac gtggttcggc
     7621 ttggaattta aaatcttcga tatctttttt gattgcttct tggaaagcag gaatcacaga
     7681 ttcagctgtt tcttttgttt cgatcaatgc gcttttaaaa tgattcaggc tgttatttaa
     7741 atcatttgtc agagttgttg cttcttccaa ttcttcgatc agtttctttc ttgtttcatt
     7801 cccgctcctg ggcgccaata acagacctcc gacagtgcca gcagcagctc caaataacag
     7861 tcctttgaaa aagtttttaa tcatcgcagg acacctgctt tacgattgtt tcttgaatag
     7921 cttttagttc ttccgatcca taactatctt gatgattgac aaagtgaata gaaaaatcat
     7981 cctctttgct gtagcgaggg actaaatgga tatgtgaatg aaaaacagat tgataggcta
     8041 gttcacggtt gttattcaag atattaagac ctttcatttc tggaaaagct ttttccaatg
     8101 cttgagcaat cttaggcaca cgcgaaaaaa cctctcctgc aagttcggga tcatattcaa
     8161 aaatatctgc tacatgacgt ttaggcacaa ctaatgtgtg tcctttcgtt gcttgagaga
     8221 tgtctaaaaa agcatagacc ttgtcgtctt catatatttt atagcttggg atttcttgat
     8281 tgatgatttt gcaaaaaata cagttttcca tataaaacat cctttctata aagaataaat
     8341 acttcagctt aactttacca taagttaaag ctattgtaag gaaaaaaaca aaataccgtc
     8401 attttcttcg tggtataata aataaaaata aatggcggag gaaaaagaat gagtttaatt
     8461 atcgatcatg tcacaggggg gtacggacat ctccctgtgt taaaagatat cagtttcgat
     8521 gtacagcctg gagagatagt agggttgatt ggactgaacg gagcagggaa aagtacgacc
     8581 atcaagcaca ttatcggtct gctggaagct tcaaaaggaa cgatagaaat caatcatcta
     8641 tccttaaaga aaaatacgga agaatatcgc aaacaaattg ggtatattcc agagactcct
     8701 tctttatatg aagaattgac attgagagaa catatcgaag tcactgcgat ggcctacgat
     8761 atacctatgg atatagcaat gaaaagagca gaaaagttgt tggagacttt tcggttatct
     8821 aatcgtcttg actggttccc agctaatttt tcaaagggaa tgaaacagaa agtgatgatc
     8881 ttatgcgcct ttttgatcga gccttcttta tatatcatcg acgaaccatt tttaggcttg
     8941 gatccactag ctatcaatgc attgcttgaa ttgatggtag aaatgaaaaa agaaggagcc
     9001 ggtattttga tgtctacgca tattttagct actgcagaaa aatattgtga tcgttttgtg
     9061 gtcttacata acggagagat acgagcagac ggttctcttg agatgttgag aaaagaattt
     9121 cagctgccgg attcttcttt ggacgaaatc tatattgctt taacaaagga gcaggcacat
     9181 gagtagtttt tttcaaaaac gtttagcaag acatcaaaag aaaatgatga aatatatgcg
     9241 ttatgtattg aacgatcatt ttgttttagt ttgtcttttc ttgattggcg gtgtgggatt
     9301 atattattct aattggttga aaaccctgca gccaccgttt gcaattggag ggctcataat
     9361 ctctgtattt tggatgctct gtttattcgt gggtaagatg gctacttttg cagaggcagc
     9421 agatattgtt tttttacttc ctaaagaaaa agaaatgcaa acctatttgg aacgagcttt
     9481 tcgctattct tgcatttttc cgttgatctt tttgatattg gcatgcggat tggccatgcc
     9541 attagtggtg gttagtacgg agcaatcctt ttcgatgttt ttcgtctatc tgatcatatt
     9601 atggagtttg aaattcagtc atctgcaaat caagagagca gaattgtttc gactagatca
     9661 acaagttatt cgttattgga aattaggctg gtttttttct tctctgatct ttttgtttct
     9721 atcattttat tggctaatat gggcaggaat agcaggtgca ttagtacagg caagtctata
     9781 ttactggttt ttgtggaagc aacaagaaaa aggattggat tgggagaaaa tggtgcatgt
     9841 agaagaacat aggttgcatc agatctatca atttatcaat ttattcacag atgttcctga
     9901 aattacggca aagatcaaaa gaagacgcta tttggatcct atattgactc atattaaaag
     9961 agaatcaaaa aacacttatc tttacttgtt tgcaagaaga ttggcaagag gttctgattt
    10021 tagtggtctt tatttccgat tagtaattat aggtgggtta ttgattgccg gagtagtcga
    10081 cttttatttt agtctgatta tcggtttctt atttttatat ttgatcggtt tccaactgtt
    10141 acctttagcg aaccagttcc gctatatggt tctcgttcag ctttatccaa tccataagaa
    10201 tcagaagaag caggcgattc agtatctact cacatgggta ttaattatcg cgtcggttat
    10261 ttttggtttg attgctgcag tcgtattaga tgggcagcag cgatgggttc cgctagcggc
    10321 ttatttgatt gttactatgc tctttgttaa actatatgta ccaactcgtt tgaagaagat
    10381 gtcagactga aaacaaagct gtgtagggta aaaatgagaa tgaaaaatag agcgttgaca
    10441 gtaagaatgc tgttcgttct attttttttg agaaaagctg gttttttgaa tggcaattaa
    10501 atgaaaaaga acgtatatgt gcttgacgtg tagtgttcag gcgactagaa taataagtac
    10561 tgcaaaacaa agagtaagag gacgggagaa tgcatgatgg agtttcagtt agaccaagag
    10621 tggcggttac gtcccatcaa aggcgacaca ggaaaaacat acataggcat gcgtgatgag
    10681 gacaaagtct ttattaaacg aaacacgact ccgatgttgg ctgctctttc aagggaagga
    10741 attacaccaa aacttgtttg gacaaaacga acggggaatg gcgatacttt gacagcacaa
    10801 gagtggctgg aaggtcgctt gctgactcct aatgagattg gtcaaagaaa cgacgtgatt
    10861 gatgttttgt accatttaca tcattcaaat tctcttaaaa ccatgctgac ccgtatcggt
    10921 ggtcgtgtca tgacgccgga gaagatgctg caaaaatatg aaaaagaatt accggaagaa
    10981 ctccagaaaa atcaatttct gcaaattgtc catcgttttc ttcgtgagca gatcccgtca
    11041 atagataagg aaaacttatc agtagtacac ggagatgtaa accatcgtaa ttggttagta
    11101 tgtaaaaatt atttatatct agtcgattgg gattcaatca tgtttgcaga ccctgcggtg
    11161 gatattgcaa cgattttagg aaattatgtg ccactgtcta gctggaatca gtggctgatc
    11221 agttatggca tacgcccgac tgacgaaatg cttgaaaaat ttcattggta tgcggccatg
    11281 aatttgctcc aagaaatcac cagatattgc aaacgtgaag atcatcgacg gatgaacgaa
    11341 gaaattttac aattaaaacg aattttcagt ggctgaggtt tggcccaaac tctgcggaga
    11401 tttggccaga cctttttaat tagaggaaag gaatggagat aaataatgcg tgtccgaaat
    11461 cgtccgggag cagcggaaat gttagcggct cacccgaatt ttgtaattag tgatccaaca
    11521 ttgtggaagg gaaaatggaa tgaattgttt gaaaacgatc atccaatcca cattgagatt
    11581 ggtatgggga aaggccagtt catcactgga atggcaaaag cccatcctga aatcaattat
    11641 attggagtag aaatgcaggt cagtgttgtt tcgattgcac tagacaagtt gatcgaacaa
    11701 ccgcttccta acctgaaact tctacatgtg gatggatcag cgttgacaga gtatttcgct
    11761 gacagcgaag tagatcaaat ctatctgaat ttttccgatc catggccaaa aaaacgtcat
    11821 gaaaaacggc gattgacata taagactttt cttgctgtag atgaacagat tcttcgtcca
    11881 aatggagaaa tccatttcaa aacagataat caaggattgt ttgagtattc tctggcaagt
    11941 ttttctcaat atggcatgat cctcaaacag gtatggttag atttacatca aagtcaattt
    12001 gaaggcaata ttatgacgga gtatgaagaa aaattttctt caaaaggtca acgtatttat
    12061 cgagtagaag ctcaatttca agataagacc aaaaagaaaa gtctgtgaaa ctaactttct
    12121 gctttcataa aaacattaat atccataaat aaatctcaga aaatgaacat tacagatgta
    12181 atgtattttc tgggatttat ttgatttaaa atgtatttat ttcatcggtt tttccacaag
    12241 gtggaatcaa ttggaagcga gaaaatgaaa ataatccttt gtcggtgatc ttcaattcgg
    12301 gtatgacaga taatgttaag aaagaaagtg tcaaaaaagg atcaaaggtt ttagctgctc
    12361 ctagttgatg tgctttagtt attaattcat tcaactgttc atagacgatc gaaaaaggtt
    12421 cctgtgtcat gattccgcct ataggaagag aaacacaagc tatttcctgc tgtttgttga
    12481 caacggtcat cccgccacct ttttgaatca aaagattagc agcatacagc atatcctcgt
    12541 cgtttgtccc aacgacaact agattatgcg agtcatgggc aatcgttgtc gctatcgctc
    12601 cttgtttcag ctgaaatcct ttcactatgc ctaatccaat gtttcccgtt tgatggtgac
    12661 gctcgatcac cgcaatcttt aattggtctt tttcgattga aggaatgaat cgttcgcctt
    12721 gtcggtccac tttttctacc aaatctttag tcactagact attcgggata atttcgatca
    12781 catgggcttg tgaagaagaa agaagcagct ctaacgaatg tggatttaat ggttgagcgt
    12841 ttagttttgg cagacaacac gtatatgaat ttgaatgatc agcaaataaa tggtcttgga
    12901 gttgcccaga ttgaacaact aatgttccct tatagaaaac ttcatgaatt ggcagcttgg
    12961 taagatcgtc tgttaagaag aaatcagctt gatacccagg ggcaatcgca cctagtgttt
    13021 tcaaatcaaa acattctgct gcattcaatg ttgccatctg aatggcttgt aatggatcga
    13081 ttccaagtgc aatagcttga cggacgatat gatcgattga tccttctttt actaaatcat
    13141 tgatcagttt atcatcggtg acaaagaagc atcttcttga attttcaggt gtgatcgccg
    13201 gtagtagcga tgccagatct tttgcaacgg tcccttctcg aaccattaaa tacataccaa
    13261 gatccaaacg ttctttagct tcttttgcag ttgtagcttc atgatccgtc cgaatacctg
    13321 ctgcaggata aacatttaac tcttccatgc cgataccagc tgcatgcccg tcaatttttt
    13381 tcttatgctg gtgcatcaat tggatctttt ctaggatgtc tgtttcatta tttgctactg
    13441 cttgatagtt catgacttca gctaagccta aaacttcggg tttttgtaaa aaaggggcta
    13501 atttttttgc atttaaattt gcaccattgg tctcaaaagg ggttacaggt acacaagaag
    13561 gtagcataaa aaagatattc atgagtgttt gtcttgcatc ttctatcata aactcaagac
    13621 ctgtactccc agcaatgttt cctatttcat ggggatctgt cactacagta gtaactccat
    13681 gaagcaagga tacttttgaa aattcgcttg gtgataaaag tgagctttcg atatgcacat
    13741 ggccatcgat gaatcctggc actagatatt gcccattagc attatgtacg ctttttcctt
    13801 cgtattcacc gattcctacg atcattcctt cgcagattgc tacatctttt tccatgattt
    13861 ctttcgtaaa gacgttgacg atcttagcat gcttgatcac taggtcagca ggcttttttt
    13921 tacttgctac ttcgatatat ttccttaatg ttgtttccaa tggagtgcct cccaagataa
    13981 atttttataa gtataacata aattcggctt ttgccaatat ttttttgtaa attcatttta
    14041 tttgaaacaa aaagatcaaa aagacgtatg ttttttgtgg aggggagaat aatggaaata
    14101 aaacgattat cggatcaaat gataagtgaa agttttgcca aacatgccca agacgtatat
    14161 atattgccag cttcagaagt attttacatc tattttcgct atggaaaaaa acgaacattc
    14221 tggaagaaag tctcttcggc agatggacag caactgatct caagatttaa gtatttggga
    14281 cagatggata tcggagaaaa aagaaaagct cagttaggtg catttactta ttctttagaa
    14341 aaacaggaag ttcgtctgcg gttgtcttct gtaggagatt accttcaacg agagagttta
    14401 gtgattcggc tattatacgg tacttttgaa acaccccatc gttgctttag ttctcgagat
    14461 tttgcaacta ttttacagaa aaccgtcaga cgtggattat accttttcag cggaccagtc
    14521 ggatcaggca agacttctct aatgtatcgg ttggctttgg aagaaaaatt gcaagtgatt
    14581 actatcgaag atcctgtaga gatagaagaa ccgttttttc tgcagctaca gatcaatccg
    14641 aaaatagcac agacttatga tacacttttg aaactcgcat tacggcatcg tccagacttg
    14701 ctaatcattg gcgagataag agatgaatca actgcacatg ctgcggtaag agcagcacta
    14761 acagggcacc gggtttttgc aaccgtacat gcacgcaatc ttgaaggtac attggtcaga
    14821 atccatgaac tgacaaagaa aaaagaagag ctatctgaat gtttagctgg tgtcatttat
    14881 caagaacttt tggaagatgc caatcaccat ccttctgttt tatggagtta tcaatttatg
    14941 gaggaagaat cagtaaaaaa atcatgggag gaaagttatg aagaagctca ggaaaatatt
    15001 tggcagtcag aactattcta aaggattaac aagaagccag caaaaccagt ttgcacaaac
    15061 catgggagaa ctgcttttat ctggttttag tctgcaagaa tccctttatt tattactcac
    15121 gatccgttcg tttccacctg ttattttggc aaatatccaa catcagctaa aggaagggca
    15181 agcgtttagt gaggtacttt cgcaaatagg atatggagca gagcaactgg tacagatcga
    15241 gttagctgaa cgtcatggga atctgccgac aactttgttg gaaattgcca aacagtttcg
    15301 gcttgttgaa ggatttcgca atgatttgaa aaagctgctt gcatatccga tgctattgct
    15361 cacctttttg ctaggtatct tgtttagtat gcggttgttc gttttgcctc agcttattgg
    15421 ttcaggaatg gtcatgcaag agcattgggg tattcagctg atacaagtct ttcattggta
    15481 tgttctacta ttcatcctag cggttactct attggcttta ctagtaaggt atcgtctgat
    15541 gaagttgtct cctgttgatc agagtgaatg gataagcaga caattattga ttggaccgat
    15601 atattccttg tatcaatcgg cttatttgtc actagagatt ggaaagcttt ttcatgaagg
    15661 tttcgaatta aggcaaatca tagtctgctt aaaggaaacg aaacaagaaa gtctgattca
    15721 gcatcttgcc tctaagttga ttcaaggatt ggaggcaggt atgtcattgg ctgagcagtt
    15781 ccagaattat cgttttctat cagaagaatt cagtaaaatt gttttacagg gagaggcaaa
    15841 gggaagttta ggtaaagaac tgatttttta cagcgaatgg acaagaaaac agcttttcaa
    15901 acgtcttcat cactggatgc attttattca gccaatcatt tttttgggcg tagcggcttt
    15961 gattttatta gtttatgccg ctgttttatt acctgtttac ggaagcatag aggaggtttt
    16021 gccatgaaaa agagaaaaat atcttattat agcggtttta cattgatcga aatgctaatc
    16081 gtcttactga tcatcagtgt attagtgttg ttgttcgtcc ctaatttgtc gcgttatagg
    16141 aatcatgtag accaagaaag tcgtgaagcg atcatccagt tggtagacac acaaaaagag
    16201 ctctatgcgc ttcaaaataa tggaagagtt cctactgtgg aggaattgtt gaatgaaggc
    16261 tatatcaaac gagaacatgc agaaatctat cagcggcctt gaaggtaagg ttttagcatc
    16321 aggatatacg ttgtttgaat cactcttact gattttatta cttacaacga tcctttcttt
    16381 tccagttctc gcattttctg catggaagaa tgaattggca gtctatcaat ttttcggcca
    16441 atttgaaaaa cgaatctatt ctacgcaaaa aatcgctatt gtgaatcaga tccaaacagg
    16501 cttttattgg aatagtgaag agaatcaaat catttttcat gttccgaatc cagataaaat
    16561 aaattgggaa gtattagata ttcctgatga gattaccttg aaacgccatg cttctattac
    16621 ttttgctgct agaaccggga atgaaagttc actaaaagct tatcaatttt attgggaaga
    16681 aaaagagcgg acgattacgt atcaattcca gatagggagt ggtcgctata caaaaagaat
    16741 tgaataaaac aggatttgtt ttgttggagt cccttacagc gcttatgatc gcttctgtct
    16801 gtgtctactt agtgagcttt ggccagctgt ttttgcttca ggcagaaaag aaaagtgggg
    16861 aggaactgct atttattcga atgatttatg aagatatcaa gacgcagcgg atgtatcaca
    16921 aaagaccaga gcgaagggaa gaaatgatcc catacgatat ccaaattgtc gataataatg
    16981 gagtaatcga aaaagtgttt gtcaatatgg agaaagaagc gatcagtatt gaaaaaacaa
    17041 gtaattccag catttacgct gattgaatgt ctaattggac tgatgatatt gagcagtcta
    17101 ttgctcacat tcaatctttt gattcagcaa accatccaag taagtgaatt tttgcagcgt
    17161 aaagatcaaa aagagtggct aatattttta gcacaattag aagaagaact caagagcagt
    17221 cacaatgtct ctgtgaaaaa tcagcaactg cactacacga tcgaggataa acagtatatc
    17281 atcgaaagat atcaggcaat gatacgtaaa agaagaactt ccggcggaca tcaacctatg
    17341 ttaacaagta tcactgagct tcaattattg gaaaaagaac acacaatttc tttttatgta
    17401 cactttgaaa atggagaaga tggatatgcc atatggacaa aaaacgatca gtagaaaatg
    17461 ttataagggc tctattttat tttctgtctt gcttttattt cttttatgta cgtttttatt
    17521 gatggcagca ttagaaagct accgtttgtc aatggatctt aatatacgaa tcaaaaatta
    17581 ttatatagca aaaataatga agcagatggc tttatcggaa cagtctacgc ttgccgaaag
    17641 ttcggaagga gttttttact acacaactgg atctgtaaca tatcagtggg tacaacagtc
    17701 cctattctta gaggtggaag tctcaccgtt tatctttcgg ttcacagaag aagtaaaaaa
    17761 tgatacagat actagtacag aatgatggaa tgttcgtgaa aacgccctct tttctgttat
    17821 aacactgttt ttaataaaaa aatgtttgcc gtttcacaaa taaaaaacag tgaaaacgat
    17881 tgaaattttg tcaattgttc gatagaatta aacaggtgaa aaaattgtta taatatatct
    17941 agaaatgagg tgcaccatgt taccagaaaa aatggaacaa gggtttaatc aaaacctgga
    18001 agctattcag cttttacaga atgcattggg aacttccttt ttagaagcct atgtcgaaaa
    18061 tgcagaaaat ttaatagatg attatcaagt acgtgtggtg gatggagtgc cgacaaaaga
    18121 gacaacgcaa cggatcacag ctctttatga agagttgaag aaactttctt tcgagccaga
    18181 agaatggcgc agactgtctc aattactttt attgaagggc agccagacag aacatttgca
    18241 ggcgaaccat caactcactc ctgatagtat cggtttttta tttgtttttc ttattgaaca
    18301 actatacaca aataaaaaag caccggttaa gattctggat attgcggcag gaatgggcaa
    18361 cttgttatta acagttcttt taaacctatc gaatgcgggg taccaaacag aaggaattgg
    18421 tgtggatatt gatgatacat tgttggctgt cgcagcttct acaagtgatt tgacacaagc
    18481 aaatgttcaa tactttcatc aagacgggtt gcaggaatta ttaatagatc ctgtcgattt
    18541 tgcaatcagc gatttgccaa tcggttatta tccgaatgac gaaaaagcaa aagagttttt
    18601 aacaagtacc gaagaaggcc atagttatgc acatcattta ttgctagagc aatcaatgaa
    18661 atatgtgaag ccggatggtt ttggtttgtt tttgatgccg agtggatttt tagaaacgga
    18721 tcaaagtgaa gaaatcaaaa aatggtttaa agaagaaggt tatcttcaag gaatgattca
    18781 attgccagat gaattgttcc gtaataaaca atcgcaaaaa agtattttga tcttacagaa
    18841 aaaaggacca caagcacaac aagtaaaaga agtcttgctt gtgaaacttg cttcattaaa
    18901 agaaccagag aaggtgacgg aatttttcaa cgagtttaaa aactggaaat catctgctct
    18961 gtaatcattc aacgagaagg agagtcatat gtctaaaaca attgcaatca atgctgggag
    19021 ttcaagtttg aaatggcaat tatatcaaat gccagaagaa accgtagttg cgaaagggat
    19081 cgtcgaacgt atcggtttac aagattctat ttttaccatc aagtacggcg ataatcaaaa
    19141 attcgaacaa atcatggata tcgaaaacca cgaagtagca gtaaaaatgc tgttagatca
    19201 gttgattgaa ctgcaaattt taagctctta tgatgaaatc accggagtag ggcaccgtgt
    19261 tgttcacgga ggagaaactt atggggattc agtagtgatc gatgagaaag taatggaccg
    19321 cattgctgaa ttagcagaat ttgctccttt gcataatcct gcaaacttaa tggggatcaa
    19381 agcgtttaaa aagattttac ctgacatttt gagtgtggct gtattcgata catctttcca
    19441 ctcaacaatg ccaaagcaaa attatttata tagcttaccg gtgtcttatt atgaagattt
    19501 caaagtaaga aaatatggct tccatggcac aagtcatcgt tatgtgtcgg aacgtgcggc
    19561 aaaattattg aacaaaccga tcgaagaact aaaaatcatc acttgccact taggaaatgg
    19621 tgtatcgatc acagcagtcg atggaggtaa atccatggat acttctatgg gattcacacc
    19681 attagctggg gtgacaatgg gtacacgttc aggtgatatc gatccagctg ttctcccata
    19741 tttaatggaa aaattagaga ttgatattga tgaaatgatc aatgttttga acaagaaatc
    19801 tggtctgcta ggcctaacag gattgtctag tgatatgcgt gatcttgaaa agaactatga
    19861 aaaagaacat attcgtttag cttatgacat tttcgtggac cgcatccgaa aatacatcgg
    19921 cggttatgtg acaacgatga atggtgttga tgcaattgtc tttactgctg gtatcggcga
    19981 gaatgacgga catgtacgta gtgaaatcat caaaggtatg tcttggtttg gttgtgaaat
    20041 cgatccagcg ttgaatgaat tgcgtggtga agaagtagat atctctacaa aagaatcaaa
    20101 agtaagagta ttagtgatcc caacagatga ggaactaatg attgcccgtg atgtggagcg
    20161 tctgcgcggc taagccaaag cattcgttag gataaaagat gaaacgcatg caagagggaa
    20221 ttcttgcatg tgtttttttt gcggagaaga ccgcaaaatg gtaatttaac ttcaatttcc
    20281 cttctttttt acaggatctt cttagggaat tttgcttgga aaagtggtca aaagggaaaa
    20341 gactggcatt ttatttattg attttcagga aaatcccaat attttatttt cattcatctt
    20401 tataagggac attacatatg taaaaagaaa aaacattttt agatcagcct cgtgggctgt
    20461 cgactttatt cttcgcttta tgtttagcta gaaaaccgat ccgaaacatt cattgatcga
    20521 ataataaaaa cttgattttc cacctgtact ttgcctgaat cgtgaaagca caggtatgaa
    20581 aatcaagttt tttgttgcga aaaaacgtcc tagtcaatca gttggccgag cggctggcgg
    20641 ctgatctgct cctgatcaat aacgagtgct tcttggaagt gttgctgcaa aaagtagcgg
    20701 tcatggctga ctgctaggag cgttccttga tagtcttgca gcatttcttc aatttcttct
    20761 cgagcatagc tgtcgaggtg attggttggt tcatccaaaa tcaggacgtt gattttttct
    20821 aaaaagagtt tcagcaacgc caaacggatt ttttcaccac cggataaatc tttgatccgt
    20881 ttacccacat cgctttgata aaagccactt ttggctaatt gccgtcgagc gacttcttcg
    20941 tttccagtca atgtgatggc gtaagccagc aaacggctat cgggatcggc aaactgcagc
    21001 tgctgcggca aataaccgat ttgcaagcta ctacctaatt tgatggtgcc accgtaaggc
    21061 ttttcctcac cgagtaataa tttgagtaag gttgattttc ctgcaccatt gtcgccgatg
    21121 atggctaaat gatctccacg atagactgca aaactgctgt ctttgaatag tacttgatca
    21181 gcgtactgct ggtgcaagtt ttgaatgatg aaaacttctt tgccagattt tccaccatta
    21241 gacaagcttt gcagccgatt tttcggggga ggtggcggtt tgacgagtgt cagtttcgcc
    21301 aggcggcgtt ccagttcttt ggcttttttg aagaaggact cattgtctcc ttcatgtgcc
    21361 cactgacgaa aacgacgaat catgactttc aaccgctgga cttctttttg ctgcagttcg
    21421 taattttttg tcagctcagc caagcgagct tctttcaaga ctcgaaaacg gctgtagttt
    21481 ccgggatact caatcaatgc accttcttcc agttcccatg tttttgtggt gacttgatcg
    21541 agaaattgcc gatcatgaga aacgaccaga accgattttt ttgtattctg taaatagttt
    21601 tccaaccagc gaatactgtt aagatctaaa tgattggtcg gttcatcgag taaaagtaaa
    21661 tcggcatcgg ctgctaaaat tttggctaaa gcgactttaa ctcgttcgcc tccgctaagt
    21721 tctgttaacc gtgcttcctg acggtgtgga atccctaagc cttgcaagat cgctgcaatc
    21781 cggtcttcta atacgtaacc gcctagttct tcaaagcgat gctgcagctc accgtaacgg
    21841 gccagacact tctctaagtt tgtatctggt tctgtcatca atgcttcgta gcgactcatt
    21901 tgctgtttga ggtcctgtat ctcagcggca gaatggagca agtagtggtt gacggtcgct
    21961 tcggaaactg taagttcttg ttcgaccgtc ccgatcttta agcctttttt gcgactgata
    22021 acaccgctgt cgacaccttc tctgccggtc aagatttgta aaaaggttga tttgccagtt
    22081 ccgttgatac cgactaaggc aattttttca ccagcttcaa tttgacagga taaatcctca
    22141 aataatggat tcccgccgat tgttttttta atagaaattg cttgtatgat catgatctct
    22201 ctccttgaga ccactcaaaa aggccatggt tatccctggc ctgtgttatt taaactcaga
    22261 ttatcatgag aaaaatacct tgagatggcc attgatttta cgtgaattca actgttttgg
    22321 acaaacttgt cccgtcaaca gcaactgtaa aaccaattgc atggctaaat gcgatatggt
    22381 atcgttggta attttcatta cttgttattt tagtcatgcg catcatagta ttttccctcc
    22441 ttcgtgtagt tgtcttttcc agtgtacctg aaagtgtcag aaagttcaac tagcgctgac
    22501 atttacttaa tatttatcga tgtgtaattc tgtcagagag ctttttttat ctgactgagg
    22561 gtaatgtctc gtggaccaca tcattgtatt ctgagctaaa ccagcgttca ggcgaaatca
    22621 tatggtgttt gataaaaaac tttgtctaca tgacttcctt ttgccatttg cttaataccg
    22681 ttgcattaaa cagctcttct ctttggaaat ctcaccaaca tcgcgttcag gagtatcgtt
    22741 gtggttgcaa tggcaagcgg tagcctttca tcaattcttg aatgatttac cgaatgtagc
    22801 acttgccttc tcgcggtacc cgaacccgaa ataaaatttt agtttggcga taaaatcggt
    22861 atccgccatc gatacttcat attttttcgt atcagattca tcggaaactt ccacattgac
    22921 gaaccaataa gctttggcat gcttaggtcg ttttaaacca caccagggcg actaatcgcc
    22981 ctggtgttca ctttttttat tttacttgaa ctcattgact gttttccctt tattttaaac
    23041 attataaaga ccgatgagtg cttgccgcgt cttttgtttt tatcacggtt ttttgtgcaa
    23101 aaaaacctgc cgatagttgg caggtcgtct acgtgttaag ctttggtgtg gtctttagct
    23161 ggcaattggt tttctaaatc agttcggaca acaagcacat cgcaagctgc gtttctaata
    23221 acatattcag aaacagaacc aataaataaa cgttctacag cattcaatcc tgtggctcct
    23281 aacataatca aatctacttg attatcttct gggatttctc tggcaataat ttgttttggt
    23341 gagccatatt ctacgacaga ggttacatta ttcaagccag ctttcttcgc attgctttca
    23401 taatctgcca atgtttgctt tgccatttca gtagcttgtt ctgcaagcat tccatcaaag
    23461 gatgaaacgg tttggaaaga acgtgtgtca atgacatgtg ctaatagaag ttctccgtta
    23521 tttcggatag ctacattgac cgccttttta aaagctaatt cagcttcatc agaaccatct
    23581 acggcaacca tgattttttt gtattgttgt aacataagca ttcactcctc aagtcaatcg
    23641 tattgtactc ttttattgta gaacacttta gggaaaaaga gaattattta ctgctttggt
    23701 tcagcaattt tttttgcaaa tcggatactg aaggtggcag aaacaataat tacgatcagt
    23761 aaaaagacca aggcaataaa gcgttgattg aaaaaagctg ttaaaatacc cattacgcct
    23821 aaaacagcat atgcagcacc gtaaaattgt aggaatcctt gatttttatc atttttgttg
    23881 atcaatacaa aaaagatatc ctgtttatta aataaataat agctaacgaa taataatact
    23941 aaagtggcga taatcattaa aatcctaatc atgaacaaat cctccaaaat aagcaacaaa
    24001 tagaaaaagc aatttgccaa gagcaaattg ctttattctt taaaagatgg taatctccgt
    24061 taatgccgtt gtttgtcctg tagtattgat cccgcgaatt caagcaggtg ggtcccatgg
    24121 tcgtagcttc gaactaccaa atgataaggc atgttaccag cagtgacaca agacgggtcc
    24181 caagatatca ataaaaatgt tcggtcaaca cacaaagagg acaattcgaa catcacagat
    24241 ttcccacctc tatagtagca tatgaattta aaaagagcaa tgagagagct cgcagaaaca
    24301 gaaccgtttt cttgtctagt tttttggagg atgctttttc cactctttta tacggtaata
    24361 ctgttgaccc agagcctgtt catatttccc tgtatctttt ggctgataat actgagcatt
    24421 cttcagttta tctggtaaat attgctgatt gacccaagcc tgatcaaaat gatgaggata
    24481 ctgataaccc acgccacggt tgagtgattt cgcacctttg taatggctat ctctcaaatg
    24541 atcaggaaca tctccagctt tgccctctcg aatatcagca agtgctgcat ctaatgccat
    24601 gtaggcagaa ttggattttg gcgacaagca aagatcgacg acgacgtctg ctagtgggat
    24661 acgtgcctca ggcaatccca atttttcagc agctaataca gcgttcactg tacgagccgc
    24721 agcagcagga ttacctaaac ctatatcttc ataaccgata accatcagac gccggcaaat
    24781 acttgccaga tcacctgcct caactaaacg ggcgagataa tgaagtgcag catccacatc
    24841 acttccacga atcgattttt gaaaagccga aataacgtca taatgagcat ctccattttt
    24901 gtcatgggta agagcttttc tttggacaca ttcttcaatg atcgacagtg taagatgaat
    24961 ccttccttct ttatctgaag gagtcgatag tgtagccagt tccagcccat tcaatgcact
    25021 ccgcaaatcg ccgttcgttg cacgcgaaag gtgaagaaga gcatcttcgt ctaactggat
    25081 tgcttgttgt ccaaggccgc gttctttatc ccttaaagca tgttcgactg ccaactggat
    25141 gtcttgttct gttaatggtt tgacttcaaa gatctgtgta cggctgcgga tcgctggatt
    25201 gatggtaata taaggattct cagtagtcgc tccgatcaag atgattcggc cactttctaa
    25261 atgaggaagc agaaaatcct gttttgtttt atctaatcgg tgtacttcat ccaataagag
    25321 gatcactgta ccactcattt ttgcttcttc cgctacaact tgtagatcct tttttgaatc
    25381 tgtagcggcg tttagcatgc gaaatgcata attagtagag ccggcgatcg cacttgcgat
    25441 actcgttttt ccagtaccag gaggtccata tagaatcatg gaagagagca tgcgagcatc
    25501 gaccatgcgg cgtatgattt ttccttcccc gactagatgc tgctgaccaa cgacttcatc
    25561 gatcgttcga ggtctcatgc gataagccaa tggctgttgc ataggatttt cctccttctt
    25621 gtttttacgt cccttaatta tatcatattt agcaagatgc ctagaaagaa cagccttctg
    25681 ttgttttgtt ttcatagctt atccaactaa tattgtgtat gataaagtag gtgagaaaaa
    25741 tgacggataa acaaaagata gatgaatttt tttggaataa aacgacgcca gaagtggcac
    25801 gggatttatt ggggatgtat ttggagcatg acacaccaga aggaagatta gctggatata
    25861 ttgtagacgc ggaagcatac ctaggtccag aagacgaggc cgcccatagc tatggtttaa
    25921 gaagaacacc gcgtgtgcgg gcaatgtatg aaaaaccagg tacgatctac ctttacacga
    25981 tgcatactca tcggattttg aacatcatta ctcagccaga agggattcct caaggagtaa
    26041 tgatacgtgc aatcgaaccc gccgcaatga ttgatcaaat gagtaaaaat cgtggcggaa
    26101 aaactggtcc agatatcagc aacggtccgg gaaaattagt ggaagcatta gcaattcctc
    26161 aagaacttta tggtcagtct atagcagata gttcgttacg cttagttttt gagaaaaaaa
    26221 agacaccaaa aaagatcaat gctttgccta gaatcggtat cccaaacaaa ggtgtatgga
    26281 cagaaaagcc gttgcgtttt gttgtgtcgg gaaatcctta tatctcttta caaagaaaaa
    26341 accaaatcga gaaagattgg ggttggagga aagaaaatga aaaagaagga tctattaaca
    26401 tatttagatg aaataattga aaaacaagtg acagactatg atgtagcttt agattgggat
    26461 acaaaaaatc atacgatcga gatcgttgtt cgcttgtttg cagaaaataa agcacaattt
    26521 gagattgatg atgcagaagg gatcgtttcg caagaaccaa ttatcgaatt tgaagacggc
    26581 gtacttcttt ttaatcctca gaaatctgtt tttgatgaac gcaattatct agcagtaatc
    26641 ccttatgaag gaaaaaaagg gttggcaaaa tcagtagctg attctttagt ggaatatcta
    26701 aatgaagtat tagctcaagg gcaaagcgat ttgcttgatt ttttggatga agacgatgac
    26761 gaagctgtat tcgagcttca ctgggataat caacaactag cagaattagt agaagtaaaa
    26821 cagcaaaatg aagcagatac ttatttaccg tacccaagtt attaaggagt gacagatatg
    26881 aaatggaatg aagtaaaagt agaaactgaa agtgaagcag tcgaagcagt tgctaatatt
    26941 ttgatggagg caggggcgag tggtgtcgcg attgaagatt ctttagatgt tgaacatttt
    27001 cagtcggatc aatatggtga gattttagat aaaaatgatt ttacatcgct gaaagaaggc
    27061 gcactagtca tggcttattt tccggaaacg actttcttac cggaaatctt gccttttatc
    27121 aaagatcgta tcacacaatt acctgagttt ggtctagcta ttggaaaaaa taaagtaaca
    27181 gttagtgaag tagaggaaag caactgggca acagcctgga agaaatacta tcatccggtg
    27241 cgtattacgc gaatgctgac ggttgcgcca agctgggaaa actaccagac agaagatcct
    27301 ctagaaaaaa ttatcacact agatcctggt atggcatttg gcacaggcac tcatcccacg
    27361 acttccttga cattgacagc actagaaaca gtcttgcgtg gtggtgaaac attgctagat
    27421 gtgggaactg ggtcgggcgt gctaagtatt gcagcaaaat atcttggtgc aaaagatgtc
    27481 tatgcctttg atttagacga agtagccgtt cgttcggcga aagaaaatat ggatatgaat
    27541 gaaatagcca aagatgtcca tgtatcagct aacgatcttt tgacaggaat cgagattgaa
    27601 agtgatgtaa tcgttgcgaa cattttagca gatatcatct tattgatgat cccagatgct
    27661 tggcgtttac tgaagcaaac aggcacgctg atcgtttcag gaatcattga agagaagaag
    27721 caaatggttc tggatgccat gttagaacaa gggtttatcg tggatcaaat tttccagcaa
    27781 aaagactggt atgcaattat tttgaaaaaa ccagaggtgg aatagatgca gcggtatttt
    27841 ttaaaagaat catatgaatc aaaagactat tttgttttag aaggggaaaa ctatcaccac
    27901 attgtacggg ttatgaggat ggaacccgag gatgaagttt ttttggcatt caatgatcaa
    27961 atagccattg ttgcagaaat cacggagatt actgaagagg ctgtttatct gaaagaaatt
    28021 aaaaaagaag aaaatcaaaa ggaactgcca agttcgatta ccatagcgag tggctatcct
    28081 aaaggggaaa agctggattg gatcgttcag aaatccactg aattaggagc gcaagctttt
    28141 cttggtttcc cggcatcagc ctctgtcgtt aaatgggatg caaaaaaacg tatgaaacga
    28201 caacaacggc ttgaaaagat tgctcaagag gcagcggaac aatcccatcg gcagatcgtg
    28261 ccatcagtag agttattgga aacgaaacaa gatttatttg agcggctaga tgattatgac
    28321 gtggtcttgg ttgcttatga ggaatcagca aagcaaggcg aaaaatcaaa tgcagccaaa
    28381 attcttagtg aaacaaaacc agatagtcga atcttagctg tctttggacc agaaggtgga
    28441 ttatcccgcg atgaagtcga ttattttgta gaacacaaag ggatactttg tggattagga
    28501 ccaagaatcc ttcgtacaga aacagcacct ctttatttat taagtgctgc tagctatcaa
    28561 tgggaattaa tgaatccttc tttcagctaa cgttcttcat taataaaaga gtatcgacag
    28621 aaagaattga aaaaatagtc aacaatttgt ataatagaaa caattattta tacaatggat
    28681 acgaaaaatc gtttgaaaaa gcttttaaaa agcgaacatt tccgtaaaaa aacgtataac
    28741 tgcacggatt ttgggacaat tatcaaacag atatttgtcc cgattcccgt tttaccgaaa
    28801 aaagaaaggg ggatcgattc ctgtgacaaa agaagaaatt ttaacgggac ccggtgtcat
    28861 aaagctcgtt agtcaataca tgggtcccga acatgtagca tttgttgaga aagcatgcga
    28921 atacgcgact gctgcacatg acggacagtt tagaaaatca ggcgaaccct acattattca
    28981 tcctatccaa gtcgcaggta tattagcaga tttaaaaatg gatccccata cagtggctac
    29041 aggcttctta catgatgttg ttgaagatac agaaatcact ttagaagatc tgagagaaga
    29101 atttggcgat gacgttgcta tgttagtgga cggcgtaacc aaattaggga aaataaaata
    29161 taaatcccac gaagagcagc tagcagaaaa ccaccgaaag atgctgctag caatggctca
    29221 agatttacga gttatcatgg tcaaattagc tgatagacta cataacatgc gtacgttgaa
    29281 gcacctgcga gaagataagc aaagaaggat cgctcaggaa actttagaaa tctatgcgcc
    29341 tcttgcacat cgtctaggga tcagccggat caaatgggaa ttagaagata cagcacttcg
    29401 ttatctaaat ccaaaacagt attaccgtat cgtccacttg atgcagacga agagagaaga
    29461 acgtgaaaaa tacgtaagtg gtactgttga agatattcga atagcgacag aagagctggg
    29521 gatttttgca gaaatctatg gacggccaaa acacatttat tcaatctatc gtaaaatgaa
    29581 agatcagaaa aagcagttca acgaaattta tgacttgcta gctattcgag tgatcgtaga
    29641 ttcaatcaag gattgttatg ctgtattagg agcaatccac acaaaatgga aaccaatgcc
    29701 cggcagattt aaggattata ttgccatgcc aaaagctaat atgtaccaat ctttgcatac
    29761 taccgtgatc ggtccggcag gaaatccggt agaaattcaa atccgaacac aggaaatgca
    29821 tgaaatcgct gaattcgggg ttgctgcaca ctgggcctat aaagaaggaa aaaatgaaaa
    29881 agtagaacca gatggtatga cgaaacaatt aagctggttc catgagatac tcgaacttca
    29941 agacgaaagc tatgatgctt ctgaatttat ggaaggcgta aaaggagata tctttagtga
    30001 taaagtctac gtcttcacac caaaaggaga cgttactgag ttaccaaaag gatccggacc
    30061 attggacttt gcatacagta tccatacaga tatcggtaac aaaaccactg gtgcaaaagt
    30121 aaatggcaaa atggtgcagc ttgattacaa attgaaaaac ggagatatca ttgagatcat
    30181 gacttctcca aattcatttg gcccaagtcg cgactggttg aaattagttg ctactagcaa
    30241 agcaagaaat aagatcaaac gtttcttcaa agcccaagat cgagaagaaa atgtgatcaa
    30301 aggccacgaa tccgtggtca aatgtattac agatctagga tttacgccta aagatatttt
    30361 gacgaagaac aaactgcaag aagcactcga tcgttttaat tatcaaacag aagatgatct
    30421 ctatgcagct gtagggtatg gagaagttag ccccttgacg atggccaatc gtctgactga
    30481 aaaagaacgt aaagaacaaa aaatcgagca gcaaaagcaa gaagcagaag aaatcatgaa
    30541 tcagccgaaa aaagaacctg acaaaatgaa agtacgtcat gaaggtggcg ttgtcattca
    30601 aggggtagaa aacttactaa ttcgtatcag tcgctgctgt aatccgattc ctggtgatga
    30661 tatcgttggt tatatcacta aaggcagagg gatatccatt catcgtcgag attgtccgaa
    30721 tgttcagcct gacaaaccaa atgtagcaga acgtttgatt gaagtcgaat gggaagatac
    30781 atcgaataca cgaaaagagt atgatgcaga tttggaaatt tacggctata atcgttcagg
    30841 cttattgaat gatgtacttc aaacagtcaa tgcgctaacg aaaaatctca acagcgttga
    30901 agcacggacg aataaagata aaatggcgac gatccatttg acggttggta tccagaattt
    30961 atcccaccta aagagtatcg tggataaaat caaagcagta cctgatgtct acagtgtacg
    31021 ccggacgaat ggataggagg acagatatga gagcagttgt tcaacgggta agtaaagctg
    31081 ctgtgtcagt cgatcagcaa gaaattggaa aaattggcta cggcctactt gttttattag
    31141 gcattcatga aaccgatacg caaaaagatg tggactatct ggtaaaaaaa attgcacaaa
    31201 tgcgtatttt tgaagatgaa caaggaaaaa tgaatattag tgtagaagat gtgggaggac
    31261 aaattttaag tatctctcaa ttcacactgt ttgcggatac gaaaaaagga aatcgaccaa
    31321 gctttgtgga agccgcacga ccagaaacag ctattccttt atacgaatca ttcaatgaag
    31381 gaatcagaag tcgcggcatc acagtcgcta ccggacaatt cggagcagat atgtcggttt
    31441 ctctcgtcaa tgatggacct gtaacgatca ttattgattc taaaaacaaa taaagtaatc
    31501 tggttttcac tagtaaaaac tatgaaacac gtcgcttaag ttagactgat aaactctaac
    31561 ttaagtggct tttttgtctg caaaaagcgc ttcttttgtc agcagtcgga aaaaaagtta
    31621 agcttaaatt atacaagtta ttttttatag gaggttgctg aagatgaaag aagtaccagt
    31681 agtagatatc aaaatcacag atttaaaaaa attgtatcaa aaaggataca acatgttgga
    31741 ggagttacgc catgaagcgg acgctcccgt cgtcaaagca aagattttca ataaagaagc
    31801 tatcacgatc tacggatcat cagcagcaaa agtattttat gatccaagga atttcaaacg
    31861 aaaaggtgct atgccaaaat tagtattgaa gacacttttt ggtcaaggtg gagtacagac
    31921 tttggatgga gctgctcatc accatcggaa aaatattttt atggatctaa tgacaccgga
    31981 aagaatggaa gattatcatc gtatcttaga taagaatctg acacaagcac ttgaagcaca
    32041 gcatggtcaa tttgaactat ttgatttaag taagatggtt ttctttacat ctatttgcga
    32101 gtgggcaggt atcaatttat cagcgatttc taaagatgag gtcgaaaaat tagcagaata
    32161 tcaaatctct atgatcagcg gaacgtttac ttctccgatt gatcacataa aaggtgtcga
    32221 gaatcgcaaa aaatcagaaa aatgggcgca aggcttgatt gaagaagctc gacaaaatcc
    32281 tgtggctgga aaagaaaatg tggcattata tgcttttgcc aatgccacag atttagatgg
    32341 tcagctttta cctctggaag tggcagctgt tgaattattg aacatcatcc gtccaacggt
    32401 tgctctaact gtgtgggcgg cattgatggg acatgcactt ttcagtagac cagatttgta
    32461 tcagcaatta aaaaatgact tttcgacgtt acaagatccg tttattcaag aaatgagacg
    32521 ctattatcct ttcttcccaa tgctacctgc gatttcgctg aaagaagtag aagtagatgg
    32581 ttatcgtata ccagaaggca gttgggttat tttagattta tatggaacag accatgatga
    32641 acgaacagtt gaagcgccag attcctttat gatcaaacgg tatgtaggga aagcaaaaga
    32701 tatttcctat aaagaagaat atgagatgat tgcacaaggc gggggaaatt ttcgacaaat
    32761 gcatcgctgt gcaggagaat ggatcactct tcatagtcta cgtgtgtttt ccgatcagct
    32821 agtaaataag tttgagttct ctgtacctga acaagattgg acgattccat tcaatcagtt
    32881 tccgacttat ccgaatagcc gagcgctact ttacaagaat tagattagca taaacttatc
    32941 gaaaaaaagg aagatcaaac agtgctgatc ttcctttttt catattacta taaattgata
    33001 aggtcaattg atcaaaatcc acgaaatgct tcatcaatgg tttgataatc ttcattggat
    33061 aaatgcaaat ctaaagcttg gacattgtca gatacttgct ctggacgctt agctccaggt
    33121 ataactacag acactcgtgg attcttcatg taccaagcta aaacaagttg cgcaggagtg
    33181 gcttggtatc ttttagcaat ggggcgaagt acatcgacag cggttaaaat ttctctgaaa
    33241 cgttctcctt ggaaatctgg atttcttttt cgaggatctc cttcaccaaa ttgtttttcc
    33301 tcaccaagtt catattttcc tgtcagcaaa ccggaagcaa gaggaaaata gggaacaaaa
    33361 ctgattttgt ttttttcaag ataagggaat aattcttttt cagcctgccg atggatcaaa
    33421 ctgtatttat cttctactac atcgacatat ccatctgcat tcgcttcttt caattgttct
    33481 aacgtgaagt ttgacacacc gactgctcgg atttttcctg cttcttttaa ttcgtgcaat
    33541 gtagcaactg attcattttt tggtgtgctt tcatccggaa agtggatata aaaaatgtcg
    33601 atgtaatcag tttgcagacg ttttagtgca tcttccaccg attgtttgag aaactcagga
    33661 gaattatcaa atgttcgccc tttatttggt acatgagcag cttttgtagc gatgacgaca
    33721 cgtgagcgat catattcttt ttccttcagt acctcgccaa tcagttcttc ggaacggccg
    33781 tttccgtaag cataagcggt atcgatcaaa ttgatcccgc tattcaaagc tgtacgaact
    33841 acttgttttc ctgtctcatc ttccaaaccg tcaaaaagat tatgtcctcc aacggcattt
    33901 gctcctaatc caagctgttc tgcgtagact tgtgaatgtc cgattcttat ttctgccata
    33961 gctgattcct cctttttgtc tagtctaaaa caaaatacca aaaagctgaa ctaaaacgtt
    34021 tttgtgaggg gaaatagtca gatatctctt gactttgaga ctggttttta gtaggttata
    34081 agtaatatcg aatgtcaatg aaagagcgag taagtttttg aaactttaca agagaggatc
    34141 atcgaggctg aaaatgatct aaagtttcat tagcctaaag acactctgga gatggacgag
    34201 tgaaaagccg tcccgcgtac gagcgttatt cgtaagagga aagagaaatc tttcaaaatt
    34261 aggtggtacc gtgtatgaat ttcattcacc cttgttcatg atgaacaagg gtgatttttt
    34321 tatttacata gctaagaaag ggagagaaaa ctatgagttt ccaaagacca aaaggaacaa
    34381 atgatatttt gccaggcgaa tcagaaaaat ggcagtttgt cgaagaaaca gcacgtttat
    34441 tattcagaga ttatcagtat aatgaaatgc gtacaccgat ttttgaacac tatgaagtga
    34501 tatcccgaag tgtaggggat acaacggata tcgtttcaaa agagatgtat gatttttatg
    34561 acaaagggga acgccatgtg acgcttcgac cagaaggtac agcaccaatc gttcgttctt
    34621 ttgttgagca taaattgttc ggcccagagt atgcgaaacc gttcaaaact ttttatatgg
    34681 gaccaatgtt tcgctacgaa cgccctcaaa aaggacggtt acgtcaattc catcaaatcg
    34741 gagtagaagc atttggatca gaaaaccctg caactgatgt ggagagcatg gtgatggcac
    34801 ttgatttctt caaacagttg gggatcacgc aaatccggct agtcatcaat tcattaggtg
    34861 ataaagaaac gcgtcaagcc tatcgtcaag cattgatcga ctatctttta ccttttgaat
    34921 cagagctgag tgaagactct aaacgccgcc ttcatgaaaa tccattacgc gtattggaca
    34981 gtaaagacaa acgagatcaa aaatttgtgg cagaagctcc atctatctta gattatctaa
    35041 gcgagcctgc tcaaaaacat tttgataccg ttgtttccat gctggatgca ttggctgttc
    35101 cttatgaaat cgacagtaat atggttcgcg gactagatta ttacacacat acgatttttg
    35161 aaatcatgag tgacgcacca aaaatggggg cacaagcaac gatttgcgca ggcggacgtt
    35221 atgacaatct agtggaagaa ctaggtggtc cttcaacacc tggcttcgga tttgcaatgg
    35281 gtatcgagcg attactcttg actatggagg cagaggaagt agttatccct gcatttaatg
    35341 agttggatgc ttacgtggtc gcactcggag acgaaacaaa tatcgaagca ttgaaagtgg
    35401 tccaagcgat tcgaaacttt ggtttttcag ctgaccgcga cttcatgaac cgtaaagcaa
    35461 aagcgcaatt taagacagca gataaagcga atgccaaatt agtattggtg atcggtgaag
    35521 atgagcttgc tagtcaaacg attaatgtca aagcaatggc aagccgaaaa gaaaaaagtt
    35581 ttccattaga agagatatat gaacattttg atgaagtcta tgatgaaatg actttattcg
    35641 agtaggagga aaaaaataaa tggcacaaag aacgatctat tgcggattag tttccgaaca
    35701 acaattagaa caaacagtta cattaaaagg atgggtacaa aaaagacgag accttggggg
    35761 agtaatcttt atcgacctac gtgatcgtga aggaatcgtt caaatcgtct ttaatcctga
    35821 aatcaacaaa gaagcatggg aaatcgcgga taaatgtcgc agtgaatacg tgatcgaagt
    35881 gactggggaa gttagaagac gcggagaaaa tgcagtaaat cctaaaatgg ccactggtga
    35941 attcgaagtg attgcaagtg aaatcgtgat tttgaatcaa gcaaaaaccc cgccatttca
    36001 aattgaagat gatcaagcaa tcagtgacga aatacgtttg aaatatcgtt atttggatct
    36061 gcgtcgtcca aaaatgacta aaaacatcaa attaagaaat gcgacaacaa aagcgattcg
    36121 tcattattta gatgataatg actttctcga tatcgaaaca ccttacctag ggaaatctac
    36181 accagaaggc gcaagagact acctagttcc ttcacgtgtc catgctggac atttctatgc
    36241 gttgccgcaa tctcctcaat tgtttaaaca attgctgatg aatgctggat tcgaccgtta
    36301 ttatcaaatc gttcgttgtt tccgcgatga agatttacgt ggagaccgtc agcctgaatt
    36361 tacgcaagta gatattgaaa caacgttctt aacagcagaa gaaatccaaa cttacacaga
    36421 gggattgatt gcgaaggtaa tgaaagaagt tcgcggtatc gaagtcacat tgccatttcc
    36481 tcgtatgacc tatgatgaag caatggctcg ctacggcagc gacaaaccgg atacacgttt
    36541 tgatatggaa ttgatcgatc taagcgatac agtcaaagaa gtagaattca aagtcttcca
    36601 aatggctctt gaaaacggcg gtgtcgtgaa agcattgaat gctaaaggcg ctgcagatcg
    36661 ctactcacga aaagacatgg atcagttagg tcaatatgta ggacaattcg gagctaaggg
    36721 gcttgcatgg ctgaaagtag aagaagacgg attgaaaggt ccaattgcta aattcatggg
    36781 agaagcaaca gaagcaatca ttaaagcaac agatgcaaaa ccaggtgact tgctgatgtt
    36841 tggcgcagat aaaagtgaaa tcgtcgctgc tgcattaggt gctatccgga cacgtttagg
    36901 aaaagaatta ggactaattg atgaatcgaa attcaatttc ttatgggtaa ctgattggcc
    36961 acaatttgaa ttttcagaag aagaaggtcg atatgtctct gctcaccatc cattcacgat
    37021 gccaaaagaa gaagatatcc cgttattggc tgaagaccct gcaaaagtct atgcagaagc
    37081 ttatgatatc gtcttgaacg gttatgaatt aggcggaggt tctatccgta ttcatactcg
    37141 tgagcttcaa gaaaaagtac tagaaactct aggctttaca aaagaacaaa tggaagaaca
    37201 atttggtttc ttgttaacag ctttagatta cggattccct ccacatggcg gtattgcatt
    37261 aggattggac cgttttgcaa tgttattagc cggagaagat aatattcgtg aagtcattgc
    37321 ctttcctaaa aacggaaaag cagcagatgt gatgagtgat gcaccaagta tggtttcgcc
    37381 tttacaattg ttcgaattga atattgatgt aacgaatata gaagaatagc cgataaaaag
    37441 gtaaaaagga tttgtcgaat acgcttcttt tgacaacctt gatttaattg aaaaacactt
    37501 tataatgaag ccataacata taaagcgggg cttttccatg aaaaaaacat tcgaaaattg
    37561 ggcttatcat gattacacga ccaagctttc ggtatctatc gtttatgcgt tgatcgcatc
    37621 agtcgcattg aatttctttt atcagccagg acatatttat tcaagtggta ttactggtct
    37681 agctcagatt ttgacgactt tgtcaaaatc actgatagga ttcgaagtac cggtatcaac
    37741 aactttgtat ttactgaata tacctttgtt tgtattggcg tggttaaaaa tcgggaagaa
    37801 gtttacaatt ttcactttta tcacagtcac tttgacctcc ctttgtatcc atattttacc
    37861 ggaaacagtc ctatctaccg atccaatcat ctgtgcgttg tttggggggg ctgtgatggg
    37921 actaggtatt gggctagcgt tgagaaacgg cctatcatcg ggcggtctag atatcgtgag
    37981 tatcacgatt cgtaaaaaaa caggaaagtc gattggttct atttcgatct acttcaatgc
    38041 attgatcgtt tttgttgccg gaattctatt tggctggcaa tatatgtttt acagtacgtt
    38101 gtctattttc gtcagtggaa aagtaactga tgtcgtttat actaaacaaa agaagatgca
    38161 ggtaatgatc gtaacgaaac atccagaaaa agtagtaaat gaaatccaaa actgtatgcg
    38221 tagagggatc acgatcatca atgaagcaga aggtgcttat aagcatgata agcagacagt
    38281 attgatgacg atcgttacac gatacgaact gcctctactt agagaagcaa tgaaagcttc
    38341 tgatccacaa gcttttgtaa gtattgcaga aaacgtccag atacttggtc gtttctatga
    38401 ggatggcatt tagatcgagt ttgacaaaaa ttcatgaaat actgctgtag acagacgttc
    38461 gtttgtctgc agtttttttc taccatagta gaatcagcaa agaaaaactg cttttttatt
    38521 tgaaattttc ttaaagttga aaaaaagcat gtttattttt ttaaatttat gggatgatag
    38581 aaaaaaatag tagagaaagg acacgattct acttgctatt ttcacttaat acccgtatat
    38641 tacgaatgaa aaaagtttat gaaaagttgg aatattgaat ggaaaaacaa aaaaggttaa
    38701 aaaacagccg ctacatcact gggtttgatg ggctccgttc attagctgtg gtgggtgtta
    38761 tcttctatca ccttctgcca actagtatga aaggcggcta tttgggcgtt ccgatttttt
    38821 tcgtcgtatc tggttatttg atcacggatc tgctgcggca ggaatgggaa caaaatggaa
    38881 aaatcaatat ttggcaattt tatgtcagaa gaatgaagag actctatccg gcacttgttt
    38941 ttatgcttgt gacagcttcg gcttatatca ctttgtttca gcgagggctg ttaaacaatc
    39001 ttcgaggagt tgtggtcagc agcttactgt atgtcaacaa ctggtggcaa atcaataacg
    39061 gtttatctta tttcgatcga tttgctaatg agtcaccgtt tacgcatatc tggtctttag
    39121 cagtagaagg tcaaaactat ttggtttggc cgatcttgtt tgtattgttg atggtatttg
    39181 ttcgtaaaag aaaatggatc ttttatactg tgttaggtac atccttcctc tccgccatct
    39241 tgatgatgat cttgtataca ccaggaggcg atccaacccg tgtttattac ggtaccgaca
    39301 ctcgtctgtt ttctatatgg ttgggaagtg cgcttgcgtt tgtttggcca agtacgcgtt
    39361 taaagaaaaa catcccgaaa caagcgaaaa atattctgaa tatcgctgga ggaatctcgc
    39421 ttttggcact gattctttgc ttcttctttt tagatgatca ttacaatttc atctattacg
    39481 gaggaatgct cctagtgagt ctgttatgcg tagtgctagt tgcagtgaca gcacaccccg
    39541 gagcaagttt gaatcgatgg ctgacaaatc cagtatttac ttggattggc aaacgaagct
    39601 atggcattta tctgtatcag tttccagtta tgatttttta tgaagcaaag gtgactgata
    39661 ttgccgatca tgttttgttg catactttag tggaaatcac attgattcta atcatcagtg
    39721 aactttctta tcgatatgtg gagcgtccgc tagcacgttt tgattatggt cgtacattcc
    39781 aagtgatgaa aggatggttc agcaaaccga tcatcagtaa gagcaaacca tgggttgttc
    39841 caggcacatt acttgtagca attgcactcg ttggtttcgt tacggcaccg aaaaacgctg
    39901 taacagccga tcaaaaacaa ctgcaggcaa aaatcgctga aagtaaaaaa ttagcagaag
    39961 aaaccagaaa caattcagaa aacaccacta gttcggttga ccaagcgatc cttgataagt
    40021 acgagttgtc caaggcacaa gggaataagg cacaaaaatt agaactaacg gcatttggtg
    40081 attcagttat gttggatgct gcaacggatc tacaagaact ttatccaaaa gtagtggtag
    40141 atggagatgt cggacgacaa ttatatgcca gtgtaccgta cattgaaaaa ttaaaggaac
    40201 aagatttgtt gaaagacact gttctgattg gactaggaac gaatggctca ttctcggaag
    40261 ctcagtttga tagtgtgatg gatgcaatcg gtgatcgaaa agtatactgg gtcaatgtcc
    40321 gagtaccgac gaagcgctgg caaaatgacg tgaatgcgat gcttgaacag atgacgaaaa
    40381 aatacgacaa tatgacgctg attgattggt acagctacag taatgaccat agtgactggt
    40441 tttatgatga tcaagtccat ccaaatcctg aagggatgac acattacatt cacttagtca
    40501 gcgaagcctt gctaggagga agttcaagta cgaacagcag tgagacaaac gtgacagaat
    40561 gatagctaca aaaaacttca ggcaaaaaca tttgtttttg cctgaagttt ttttatattt
    40621 accgtgtttt taacattcat atagattcag gaagcaaaac ggttgagtgt gtctgtaaat
    40681 aacgttcttt ttgttttcgg gtgagttttt tgaatgcagc ttctgctttt gttgcttcgc
    40741 ttcttgaaga aaatacttct gcatggatca tcttaacagg tcttcttttt tgaagacgag
    40801 tatatttagc accagtacca ttgttatgtt cctctaatcg tctagaaaga tcggttgtat
    40861 atccaccata aaatgtctga tctttacaga ataatacgta aaaataatgt tcatttgcca
    40921 tagagcatct cacgaatctc tgcagtgtat tctccatcca tattgtaagt gatcaatggt
    40981 ggagcgactt tgaatccgtc taatttgcct tctttgattc cttcaactaa caaaatattt
    41041 gcctcttttc caggttttgg gtaaacaaat tggatccttt taggtgcaat tcgatagtct
    41101 tccatgacct gaatgatttc taagaaccga tcaggacgat ggaccatagt aaagcgtcca
    41161 tttgttttta gtagcttact tgaaatatcg atcacttctt ctaacgtcgt atgaatctca
    41221 tgacgggcga ttgccaggtg tttattaggg ttcgttttat ttgttggcag ccctttgaaa
    41281 taaggtggat tacagacgac taagtcacaa gaattatgtc gaacgacaga taagctgtct
    41341 ttcagatcga tggtgtggac agtcatttgt tcatttaaat ggttaagcgc gatactacgt
    41401 cttgccatgt ctgctagacg aggttgaagt tcgatagcgt cgatagccgc ctgggttcgt
    41461 ttacttaaaa aaagtccaac tgcgccattt cctgcacaaa ggtcaacaat cttccctttt
    41521 ttaggtacac gggaaaagtg agcaagcagc acagcatcta aagaaaaaga aaacactttg
    41581 gaactttgga taatcttgat atcttctgcg tataattggt cgattcgttc gtcttcgtga
    41641 agcatggatt ccctccaaaa tgaaaaaaat agtcgtctgt attatactat ttttattaaa
    41701 aagaaccaag gtggcttgtc aaacctctat tttttatgca tactatgacc ataagaaggg
    41761 aaaggaagag actatgtttt atcgatttat gcgcggggta gttcgagtac tattagcctt
    41821 agttaatgga aatgcccgct atgagaataa agaagtttta ccgcaagatg agaattacat
    41881 actagttgct ccccatcgta cttggtggga cccattatat ctggcggtag ctgcagcacc
    41941 caaggaattc agctttatgg caaaagaaga actgttcaaa aatccagttc tgcgatttat
    42001 attaactcga tccaatgctt ttccagttaa aagagaaaat ccaggaccaa gcgtcattaa
    42061 aacacctgta aagattttga aatcgacaaa tttgagtttg atcatgtttc ccagtgggac
    42121 aagacatgct acagaactta aaggcggaat ggcattgatt tccagaatgt ctaaagcgaa
    42181 aatcgtgcca gctgtatatc aaggtccgtt gacacttggg gatctgttca agcgtcagag
    42241 agtgacagtg cgtttcggcg aaccaatcga cttatcagat atcaagaaaa cggataaaga
    42301 aggtatggcg gagatcgaac gacgaacaca agcagctttt gacaaattgg acaaagaagt
    42361 gaaccctgat ttcaaatatg aagtcaaaaa aaaataaaat gataagttta tgcgtgggcg
    42421 gtcttttgaa tctactgtcc tgttcagttc agactttttc aatgaaaacg agggagagac
    42481 gtcaagtctg ccctcttttt gcgttccaat atgtaaaata gaagacaggt gtcatgtaat
    42541 ggatcaaagt gttataataa ttagaaggac attttttgag gtgattgaat gcgtttttta
    42601 catacagccg attggcatat cggaaaaaaa ctacaaggtt atgacttgat tgaagaccag
    42661 cgtcatgtac tatcttctat actagatatc gcgaaagaag aaaaagtaga ggcaatcgtg
    42721 attgccggag acttatatga taggagtgtc ccagctgttg atgccgtaga attatacaat
    42781 gaattgatgg tcgattggaa tttaaaggaa aagatcccga tttttgctat atcaggaaat
    42841 cacgatagca gtacgagact ttcagcgggt acgccatggt tcagttattc gaactatttt
    42901 ttgcgaacac agctagcaga agcatttgaa ccagtcgagt ttaaaaacgt acaatttttc
    42961 tttcttcctt actttgaacc aattgcagcg agattatatt ttgaagatga caatatccga
    43021 acgatcaagc aagcggtagc caaggtgata gataaaatgc aggagctatt tgattcagaa
    43081 aaaaaacaag tattgattag ccattttttt gtaatgggaa gtgtaaaatc tgattcggaa
    43141 acgaaaatcg aagtaggcgg gttggattcg atccctggtg aactacttac accgtttgat
    43201 tatgtagcac taggacattt gcataatcaa gctgctttga gacaggataa tgctcgctat
    43261 agcggttccc cattgaaatt ctctttatct gagatcaacc aaaaaaaagg cgtatggatc
    43321 gttgacctga caacaggcct ggagttgaat tttagggaga tcgaacccct gcatgatatt
    43381 gaagaaataa cagcgagttt caaagaattg ctggatcctg ccttttatca gtccgtcaat
    43441 cgggagaatt acatctatat ctatctcact gatagaacga tcatcccgaa tatgatgaac
    43501 cagcttcggc aaatatatcc tcgtattttg gggattgaac gggcaaacgg tcgagcagaa
    43561 caaacaaagc gcaaacgaaa agtactagaa atgaaagatc ctcaaaaatt agcagaagca
    43621 ttttttagtg aaatgacgga agagcagcta actgcaaacc aagaaaaatg gctcggagaa
    43681 acgttgcaag aaatcatgga aatggaatga tcaagaatga aaccgaaaaa attacaactt
    43741 aaaaattttg gtccttttat caatgagacc gtagattttt cacgtttgac agaagcaccg
    43801 ctttttttga tcagcgggaa gacaggagct ggcaaaacga cgatttttga tgggatcacc
    43861 ttcgcacttt ttggtgaaac ttccggaaga cttcgctctg gaaaagagat gcgttctttg
    43921 tttgctacac cagacgaaga aacaagcgtg actttttctt ttgaacatca gcagatgagc
    43981 tatgaaatcg aacgaaaacc agaacaagtt ttagcaaaac gaaaaggaaa cggaacaaaa
    44041 aaacaggcag ccaaagtaac gttgaccatt tttgatggga aaggaaaaga aataaaacaa
    44101 atcacaaaac gtacagaagc agatcagttg atcagagaac tgatgaatct cgatgcgaaa
    44161 cagttttctc aaatcgtttt actaccccaa ggagaattcc gtaatttttt ggtttcatca
    44221 agcagtgata aagagactgt tttacgtcat ttattcggta ctcaattttt ccaacagttc
    44281 aatgagcgat taaaagaaaa agcaaaacaa cagcaacaaa aattggatca tttagagcaa
    44341 gagctgcatc ttctgcaaaa aaggtttgtt ttagtcaaag aagaggaacc agcagcaggg
    44401 tttgaagccg ttttagccca atggacaaac catcaggcaa tactcgaaaa acaaatcacg
    44461 gaagaaaaag aagctctaac ggaacttcag gaaaaacaaa agcaactaga aaattcctac
    44521 tatcgttttg aaagcttgaa aaaaagttat gaagaaaaaa cgatattaat aaacaagcaa
    44581 aaggaattag ctgctcaaaa agacgaaata gaggaaaaga aacgatggat tgggtattat
    44641 gaatttacgc aacgattagt cgaaccaatc cagcgcctac aagaaataaa agttgaaaaa
    44701 atagaactgt cacaaaaaag tagtgagcaa acaaagaaac tagcgatgac tacgcagaat
    44761 tatgaagagt ggcaaaaaaa tgaagcaaac cgcgtacgcc gacaaacaaa aatcaaagaa
    44821 gctgctcaaa aactgcaaaa caaacagatg atggtgccaa ttgtcgaatc cttacgtagt
    44881 aaaaaacaag acgcagaaca attaacaatt tcattggata aagaagaatt gaatgccgag
    44941 aagctgtatc aaaatctcgc tatctatcag gaaagaactc gaattcttca agaaaattta
    45001 ctggatcagg aaaaactgca agaagatcgt cttgcttttg gacagttaaa atatctagat
    45061 gaaaagcagc aagaaataga aaaacaaaaa gcaaaagaca ctgggtcgct cgcacagata
    45121 agagaagaac tcaaaaaagc aactgaagaa ataacaaaaa atagtcagct attgaaagaa
    45181 cagacagaac aagcagaaca gctgaaaagc caatgggcaa agcagcaaat tgctcgtctt
    45241 cagctttttt tgcgtccagg ggagccatgt cttgtttgtg gatcaacgga acaccccata
    45301 caacaattaa aacacgaaca gccaaataag gaagatatct taaaagtaga acaacaactg
    45361 gcagaagctg agtctgctgt agaaagaact cagcaaatca ttgccaaaag tgaagcacat
    45421 gaaaataatc ttcaacaaca attatcagat ttagaacaaa aaatcatggc aacgaatata
    45481 caattggctg aacaagaaga acacttgcaa caggctttga aaaaggcatt tcctgagata
    45541 acagaacaaa aaacaaaaac aactcttgta gtaatcgaac aatctttaga tagacgaaaa
    45601 agcaagatgg ccaatgcaag aatagagctg gaagaaataa gacaacaagc agaaaagctc
    45661 gaacaacaat tagctgtcta caaacagcag cagacagaaa aaaataatca gctccagcaa
    45721 ctgcaaggag aaatcaaaag tttagaacaa caattgcaag atacggatag taactgttta
    45781 gaagaggaga tacgacgact tgaaaaacag ttagaagaag actcgaatca actagctgtt
    45841 gagcagaaag caggagtgca attgttgcaa gatcttcata cgcttcggac acagcttgat
    45901 cttcaacatg aacaaataga aaaaatagat gaaaaaatag aaaaggaaca agaaagaata
    45961 gctaaaaagt tagaaagcca gtcgtatttt tcaacaattg aagaaatctg tgcatttgca
    46021 caacagcaag cacaatataa agaaaaccaa cgtacagtta cttcttatca tgaagagcaa
    46081 ttagtgacag ctgaccggct gcgtcaatta gaaaatatcc agctaactga gacattccca
    46141 gatacagaag agatcagcca acagttagaa gaaataagac gtatgatcga aataaagcaa
    46201 gaaaagatat acggtttaca agaacaaaaa attagtaatc aaaaaatcta cgatgaatgt
    46261 ctttcgattt atcacacaag ccagacgaaa ttagatgagc ttacgcaatt acagcagctt
    46321 tcgcaaacat tcaacggaga aaatcctaag aagactagtt tggaacggta tgtgcttcag
    46381 gtttatcttc aagaagtgtt acaagtagca aatgatcatc tccaacgttt aacgaaaaat
    46441 cgttatcaat ttgagctagc ggataccatt ggcagttatc gagggaaaac cggattagaa
    46501 atcaatatct atgatgatca ggcaggtatg tctcgaagtg cgcataccct gtctggcggt
    46561 gagagtttta ttgcagcact ttctttagca ttagctcttg ccgatgtgat ccagacacaa
    46621 gctggtggtg tagcaatcga tgcattgttt attgatgaag gatttgggtc attagatgaa
    46681 gaagctttgg aaatggcaat ggaagcttta gaaacgatcg aaagtgaagg aagaatgatc
    46741 ggcatcatta gtcatgtaag agaattgaaa gaacgagtat tacaacaaat aagaattgat
    46801 acagaaggta gcggacaaag caaaatacgc tatcaacttg gataaaaagg gagattggta
    46861 actatgaaat ggagtattcc agaaaaagtc attgcacgtg gacgtcaata cttgcaagaa
    46921 ggccgagtgt tatcggttgt ccctgaccaa gaagaaaacg tgtggcatgc agaagtcttg
    46981 ggcagtgaat tgtatatggt tgatttggat ggtacagcaa aagagataga ttactgccaa
    47041 tgtccctatt gggaggaaca taaatattgt aagcatacag ttgctgtgga actttattta
    47101 cggtcgaaaa atctatctcg tatcatgaaa aaagaccagc caatgctaga accggtaaaa
    47161 gcaacaagtg agggagaaat cattgcaaaa ggtttttctc gtctaaaaac acaaaacaat
    47221 cctcaagcgg tgcaaccttt gattattgaa taccaagtag aaacgattga gactaatcag
    47281 tatcacccag agctttctat tttggcactt tacttgcgta tcggttcatt ggcatcaccg
    47341 aaaaaaacat atatcgtaaa aaacatctat gattttttac aagcttacac agaaaaagaa
    47401 acttacactg ttaataaaca gtaccagttt cgtctagata aacgggcgtt tcaaaaagaa
    47461 gatcaaaaaa tactcacgca tcttgcagga agtgctcaga cgcatcaatt attaggtacg
    47521 aacggcttac aagtcaaagg aaaactagat aaaagatatc tgcttctccc agtagaacaa
    47581 gcccgcttcc tgttagagca gatgaatcag actacacgtt tgcatctgca gataaatgaa
    47641 caaaacgatc aaggaataca attttccgat caaaacaaac ctttatcatt caaagtggaa
    47701 aaatttggag aagattattt gctgcaagca ttgaatgatt ttgatttttt ctttatgcat
    47761 tatcagtggg gagttatcca agggacattt tattctctga cgaaatatca acaaacggtt
    47821 tatcaaactt ggaaacaatt gattcgccga ttggagaaac cagaagtagt gttcaagaaa
    47881 aaagaattac cgtctttgtt taaagagatt atccctctgc tgtcagagat cggcgacgta
    47941 gctattgccc ctgcagtaga agaggaagta gcagatatcc ccgtcgaatt tattttcttt
    48001 ttccgaaaag caaaaggaaa aatccaagct cgagtcgatt tcgtctatgg tgaagtcatc
    48061 tattcgactg acccgaagca tgaggtctcg acagaacagg cttttcaggt gttacgtgac
    48121 acagtagaag agaaacgagt acttgatcta ttcagaatgt atcgctacca gaaaaatgaa
    48181 actggttatg aacgtccatt gccaagcggg gaagaactat atgccttttt ccgcacagaa
    48241 ctatctgtct tccgtcagtt tggtgaagtc agacttggaa gaaaattgcg agagttgtat
    48301 ttagatgcgc ataaacatca accgaaaatc gaagtaaccg agaccggttc atggctcgat
    48361 attcgattcg atgtgacagg gatagaggag caagaaatcg atcatgtcct gcaaagcttg
    48421 ctgagaaatg atgcttttta tacattagaa aatggtcagg ttctttcttt tgattcagaa
    48481 gaatttcaac aaacaagcca agtgttgcaa caattacggg aatcgatacg tacagaagaa
    48541 ggaacgatcc atgtacctaa aaatcaagga ttgatcattc aaaatcaatt ggaaaaaagc
    48601 aatgctactt ttagtgaatc gttccaaacg atggtacaag atctcattca tccagaaaga
    48661 taccaggcac agcttcccaa aggattgaat gcaacgatga gagattatca aaagcaaggt
    48721 tttcgatggt tgaagatgtt aggtcattat cagtttggcg gtattttagc cgatgaaatg
    48781 ggacttggga aaacactaca gacaattgcc tttttacttt cggaaaaaga agaacggaaa
    48841 agcttctctg ctttgatcgt agcgcctgcg agcttaattt acaactggca ggcagaggtg
    48901 agaaaattcg ccccttcttt aagtatccaa gtaatcaatg ggaataaaaa agaacgtgaa
    48961 gaattactgg ctaaagacac agatatccga gtgacttctt atgccagctt aagacaggat
    49021 ctagctaatt atcagtcgca aaaaattgat tatttgattc ttgatgaggc acaaatggtc
    49081 aaaaatagca gtacgaaaac tgcacaagca ttgagagaat tagctgtacc tcagcggttt
    49141 gcactgagtg ggacaccaat cgagaataat ttagaagaat tgtggtcatt gtttgcaacg
    49201 attatgccag gatttttccc gactaaaaca aaattcaggg aacttgccgc agaagaaatt
    49261 gcgcaaatga ttcgaccgtt tattttacgg cgagataaac aaactgtgtt gaaagacttg
    49321 ccggaaaaaa cagagatgaa tctatattct gcacttacag aagaacaaaa aactgtttat
    49381 ttagcttact tgcgtcaaat gcgtgaagaa atcacatcaa tggattctga agcattcaag
    49441 aaaaaccgga tcgggatact tgctggtctg acaagactgc gacaaatttg ctgcgatcct
    49501 cgtctgttta ttgaagatta ccaaggcggt tccggcaagc tcgaacaagt aaaagatcta
    49561 ctggtagctg ccaaagagaa caaacgaaga gttttgctgt tttctcaatt tacagggatg
    49621 ctgactatct tgcaagaaga gttagccgag ctagggatct cgacatttta tttaagaggc
    49681 agtacgaaac cacaagatcg gttaagtatg gtagacgcat ttaatgctgg cgaaaaagat
    49741 gtttttctga tttccttaaa ggctggggga actggattga atctcacggg agcagatacg
    49801 gtcatcttgt atgacctttg gtggaatcct gccattgaag aacaagctgc tggacgtgca
    49861 catcggatcg gacaaaaaaa tgtggtagaa gtgtggcgta tgatctctga aggaacgatt
    49921 gaggaacgca tggattcact ccagcaagaa aaacgcgaac tgttccaaaa agtgatccaa
    49981 ggtaatgaag aacagttgac gaaactgact gaagaagaca tccgcatgat tttaagtgta
    50041 ggagaaatcg aggagtaaga gactcgaaaa agcgttcagt aatgggtaag aaagcctttc
    50101 taaagaagcg attgtttatt tgatttaaaa aatagcaagg aggctgggat tccattttat
    50161 cccagcttcc ttgctatcat ttttttattt ctatttttaa aggttataca aagattgtct
    50221 tcattgtctc taacagctga tcaaaagaag aaatctcgat atcgcaaatt ggagtctgtt
    50281 caattttgcg ttctcgatgg ttgaaccaaa gcgcttgcca gttagcactt tttgcaccta
    50341 gaacatcatt gtcgtaatta tcgccaacgt ataaggtctc ttctggaagc atatgaaatt
    50401 ctttctcagc cagctgaaaa atctcttttt ctggtttttg gaaacctgtt gcttgagaaa
    50461 tgagcatatt gcgggaagga atccaattgt tcaattgaag ttgcttcagt ttttttgtct
    50521 gatggtcagt cggcccattt gtgataattc ctaatgggac attcttttcc ttcaagaaat
    50581 cgagggtttt tttgacctcc tcatgcaagc agatattatc aagttcttcc tcgtaaattt
    50641 tttgaaataa gaggccatct tcttccgtaa tatgagggta gtctaaatct tttagtgatt
    50701 ggctgatccg atgtgctcgc atatattcta gtgtccaatc tcctgccatt acttttggga
    50761 agttttcatc actatgatgg cggaaccgga tatatagtgg atgcatatct gcatctgaaa
    50821 caagcggaac gactcttttg accgcgtttc gaaatggctg ctgctgatca tacatcgtat
    50881 catcaacatc aaacacaact gatttaatca aaattttaca cgcctttcgg ttttcattga
    50941 ttttttcaca aatccttgac tattgtaaca gaaaaaattg tctaaaaaag ctgataaata
    51001 aaggtttttc ttactatatt tctatttcct tgacagaata aggtttttat atttcagttt
    51061 ttttcactaa aaatcgtgaa acacgtcaat aagtttgatg aacaaattaa tcattatggt
    51121 agaagtcgat tgatttattt taatgcaatc tctgtggaat tttcgaaaga tttatttttt
    51181 taatacagtc tcaat
//
LOCUS       DACDIE010000011        50704 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.011, whole genome shotgun sequence.
ACCESSION   DACDIE010000011 DACDIE010000000
VERSION     DACDIE010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50704
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.011"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            85..972
                     /locus_tag="IV250_000754"
     CDS             85..972
                     /locus_tag="IV250_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8768057.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1112..2038
                     /gene="cysK"
                     /locus_tag="IV250_000755"
     CDS             1112..2038
                     /gene="cysK"
                     /locus_tag="IV250_000755"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAP8768058.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2274..2720
                     /locus_tag="IV250_000756"
     CDS             2274..2720
                     /locus_tag="IV250_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP8768059.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KVLEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2993..4345
                     /locus_tag="IV250_000757"
     CDS             2993..4345
                     /locus_tag="IV250_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase"
                     /protein_id="HAP8768060.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4516..4803
                     /locus_tag="IV250_000758"
     CDS             4516..4803
                     /locus_tag="IV250_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8768061.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            5029..5472
                     /locus_tag="IV250_000759"
     CDS             5029..5472
                     /locus_tag="IV250_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8768062.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(5478..6662)
                     /locus_tag="IV250_000760"
     CDS             complement(5478..6662)
                     /locus_tag="IV250_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HAP8768063.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            6930..7628
                     /locus_tag="IV250_000761"
     CDS             6930..7628
                     /locus_tag="IV250_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAP8768064.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            7635..8312
                     /gene="nth"
                     /locus_tag="IV250_000762"
     CDS             7635..8312
                     /gene="nth"
                     /locus_tag="IV250_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAP8768065.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(8438..8971)
                     /locus_tag="IV250_000763"
     CDS             complement(8438..8971)
                     /locus_tag="IV250_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8768066.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(9268..11649)
                     /locus_tag="IV250_000764"
     CDS             complement(9268..11649)
                     /locus_tag="IV250_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP8768067.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(11639..12286)
                     /gene="recU"
                     /locus_tag="IV250_000765"
     CDS             complement(11639..12286)
                     /gene="recU"
                     /locus_tag="IV250_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HAP8768068.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            12350..12889
                     /locus_tag="IV250_000766"
     CDS             12350..12889
                     /locus_tag="IV250_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HAP8768069.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            12979..13398
                     /gene="gpsB"
                     /locus_tag="IV250_000767"
     CDS             12979..13398
                     /gene="gpsB"
                     /locus_tag="IV250_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HAP8768070.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            13420..13787
                     /gene="rnpB"
                     /locus_tag="IV250_000768"
     ncRNA           13420..13787
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="IV250_000768"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            13996..15162
                     /locus_tag="IV250_000769"
     CDS             13996..15162
                     /locus_tag="IV250_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAP8768071.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            15208..16704
                     /locus_tag="IV250_000770"
     CDS             15208..16704
                     /locus_tag="IV250_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAP8768072.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            16785..17468
                     /locus_tag="IV250_000771"
     CDS             16785..17468
                     /locus_tag="IV250_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8768073.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            18113..19303
                     /locus_tag="IV250_000772"
     CDS             18113..19303
                     /locus_tag="IV250_000772"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAP8768074.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            19405..20358
                     /locus_tag="IV250_000773"
     CDS             19405..20358
                     /locus_tag="IV250_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAP8768075.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSGNVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            20427..21776
                     /locus_tag="IV250_000774"
     CDS             20427..21776
                     /locus_tag="IV250_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8768076.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(21959..22282)
                     /locus_tag="IV250_000775"
     CDS             complement(21959..22282)
                     /locus_tag="IV250_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8768077.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            22411..23574
                     /locus_tag="IV250_000776"
     CDS             22411..23574
                     /locus_tag="IV250_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8768078.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            23644..24993
                     /locus_tag="IV250_000777"
     CDS             23644..24993
                     /locus_tag="IV250_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP8768079.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(25038..25469)
                     /locus_tag="IV250_000778"
     CDS             complement(25038..25469)
                     /locus_tag="IV250_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8768080.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            25857..28499
                     /gene="alaS"
                     /locus_tag="IV250_000779"
     CDS             25857..28499
                     /gene="alaS"
                     /locus_tag="IV250_000779"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAP8768081.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKEHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            28699..29295
                     /locus_tag="IV250_000780"
     CDS             28699..29295
                     /locus_tag="IV250_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP8768082.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            29417..30565
                     /locus_tag="IV250_000781"
     CDS             29417..30565
                     /locus_tag="IV250_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP8768083.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(30603..31016)
                     /locus_tag="IV250_000782"
     CDS             complement(30603..31016)
                     /locus_tag="IV250_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HAP8768084.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            31095..31526
                     /locus_tag="IV250_000783"
     CDS             31095..31526
                     /locus_tag="IV250_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HAP8768085.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(31625..31858)
                     /locus_tag="IV250_000784"
     CDS             complement(31625..31858)
                     /locus_tag="IV250_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAP8768086.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            32041..33711
                     /locus_tag="IV250_000785"
     CDS             32041..33711
                     /locus_tag="IV250_000785"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAP8768087.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            33858..34244
                     /locus_tag="IV250_000786"
     CDS             33858..34244
                     /locus_tag="IV250_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8768088.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            34231..34929
                     /locus_tag="IV250_000787"
     CDS             34231..34929
                     /locus_tag="IV250_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP8768089.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            34934..35398
                     /locus_tag="IV250_000788"
     CDS             34934..35398
                     /locus_tag="IV250_000788"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAP8768090.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            35395..36306
                     /locus_tag="IV250_000789"
     CDS             35395..36306
                     /locus_tag="IV250_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP8768091.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            36541..37080
                     /gene="pyrR"
                     /locus_tag="IV250_000790"
     CDS             36541..37080
                     /gene="pyrR"
                     /locus_tag="IV250_000790"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAP8768092.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            37094..38377
                     /locus_tag="IV250_000791"
     CDS             37094..38377
                     /locus_tag="IV250_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAP8768093.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            38390..39316
                     /locus_tag="IV250_000792"
     CDS             38390..39316
                     /locus_tag="IV250_000792"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HAP8768094.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            39335..40615
                     /locus_tag="IV250_000793"
     CDS             39335..40615
                     /locus_tag="IV250_000793"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAP8768095.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            40620..41696
                     /gene="carA"
                     /locus_tag="IV250_000794"
     CDS             40620..41696
                     /gene="carA"
                     /locus_tag="IV250_000794"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAP8768096.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            41701..44886
                     /gene="carB"
                     /locus_tag="IV250_000795"
     CDS             41701..44886
                     /gene="carB"
                     /locus_tag="IV250_000795"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAP8768097.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            44940..45716
                     /locus_tag="IV250_000796"
     CDS             44940..45716
                     /locus_tag="IV250_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAP8768098.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            45713..46645
                     /locus_tag="IV250_000797"
     CDS             45713..46645
                     /locus_tag="IV250_000797"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAP8768099.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            46642..47358
                     /gene="pyrF"
                     /locus_tag="IV250_000798"
     CDS             46642..47358
                     /gene="pyrF"
                     /locus_tag="IV250_000798"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAP8768100.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            47365..47994
                     /locus_tag="IV250_000799"
     CDS             47365..47994
                     /locus_tag="IV250_000799"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAP8768101.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            47982..48296
                     /locus_tag="IV250_000800"
     CDS             47982..48296
                     /locus_tag="IV250_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8768102.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            48334..48975
                     /locus_tag="IV250_000801"
     CDS             48334..48975
                     /locus_tag="IV250_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8768103.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            49006..49710
                     /locus_tag="IV250_000802"
     CDS             49006..49710
                     /locus_tag="IV250_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HAP8768104.1"
                     /translation="MKKNMDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKG
                     EETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSE
                     HIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVN
                     PDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPI
                     QPLNGREIYYFEEFEQ"
     gene            49707..50609
                     /locus_tag="IV250_000803"
     CDS             49707..50609
                     /locus_tag="IV250_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8768105.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
BASE COUNT        16709 a         8816 c        10917 g        14262 t
ORIGIN      
        1 aattaaaagt ataatttaat taattaagtt tgttttaaga agagaacttt ttattaagtt
       61 tttttatttt gaggaggaat ttgaatgcaa gagctgaaga cccgcagtgc ccggcaccat
      121 agacctaaaa gaaaaatggt gatcaataaa aaagcgattg ctcctttaat tggaacgagt
      181 cttttagttg ggccgatgat ggttacagcg gtaccgcaac atgttcatgc agatgaacaa
      241 ggatctcaaa cagtcgctga tcctcaagcg tttatcgatc aaatcggctg gtctgcgtca
      301 gaagttgctg caagtaatga cttgtatgct tcagtcatga tcgctcaagc gttgttggaa
      361 agtgcatatg gcacttccgg attagctagt gcgccaaatt ataatctatt cggtgtgaaa
      421 ggaagctata acggacaggt cgtttatatg gcaaccaaag aatatgtaaa cggacaatgg
      481 gtaacgatga atgagccatt ccgaaaatat tcttcttatt gggaatcctt ccaagaccat
      541 gcgaatgttc ttcgttcaac cagtttttcc acgggcgatt atcattatgc tggcgtgtgg
      601 aaaagcaata caacaagttt ttatgatgca acagcgtact tgacaggtcg ttatgctacc
      661 gatccaaatt atggggccaa attgaactca ttgattaata cttataattt aacccgtttt
      721 gatacgccat caacaggttc ggctacaaca accacaagta caacaacaac gactactacg
      781 acgacttcaa cgtcatcatc ttcaacaaca gatgtaaata caaataatgg tcagacatat
      841 acagtcgtgt caggtgatac gctatgggat attgcacaaa aatttggcat gtctgttgat
      901 gatctaatgg cgaaaaatgg catgtctatg tccagttatt ctttattagt tggtcaagaa
      961 ctaaacgttt gatttagata aaaaggcaaa gaaattacca tatatggtat atctttgtct
     1021 tttttttgct agtcaaagac gaatcactcg ccataaatga acagattgta ttatgataag
     1081 taagaagagc aaagacaagg aggaattgaa gatgacagct atctataact cagtcaccga
     1141 attgattggc aaaacgccaa tcgtcaaatt aaataaaatc gtaccagaag attcagcaga
     1201 tgtttttgta aagcttgagt tttttaatcc aggaggaagt gtaaaagatc gtattgcttt
     1261 aagtatgata gaaaaagcag aacacgatgg tctactgaaa ccaggtgata ctatcattga
     1321 acctacttct ggaaatacag gaatcggatt gtcgatggta ggagttgcaa aaggatataa
     1381 agtaatcatc gtgatgcctg agacgatgag tatcgaaaga cgtttattga tgaaagggta
     1441 cggcgcagag ttgattttga ctcctggtgc ggatggtatt tctggatcaa tcagagaagc
     1501 agaacgtttg gcaaaagaaa acggctactt tttaccatta caatttgaaa atgaagcaaa
     1561 tcctcttgtg catgagaaga cgactggtcc tgagatacat caagcatttg gagtaaatgg
     1621 cttggacgcg tttgttgcag gtatcggtac aggaggaacg atcactggcg ctggccgaga
     1681 attaaaacgt gtgtatccga aaatcgaatt aatcggggta gaaccagcag aatctgctat
     1741 tttagaaggg aaagaagcgg gacctcacaa aatccaagga atcggtacag gttttgttcc
     1801 taaaacactg gatacttctg tgtatgataa agtcctttct attagtggag acgaagcgat
     1861 ggaaacagct agagaagttg ggcgaaaaga agggatttta gtaggcatct catctggcgc
     1921 tgcaattgct gccgcgctaa aagtcgcaaa agaattagga aaaggcaaga aggtattagc
     1981 tgtggttccc gacaatggtg aacgttattt atctacagcc ctttaccaag agatataagg
     2041 cacttggttt ttctccagat aggtttatgt aaaaaacttt taaagatgaa aagaacacac
     2101 aaaaatttat gatgagaaaa attatttgaa tagagacgta gaatagtgaa cgcgactgtt
     2161 ttacgtcttt tttcattaaa atacaataaa attgaatttt cttttcatgt taagtggatt
     2221 ttaaaatgta aaaatagtat gataagacga gaataacaac gtggggtgtt tacatggaaa
     2281 ataagttagt cgaagaggtt atcgaaaagt taaaaaaaga gaacattcga atcacacctc
     2341 aacggtacgc ggttttagaa tatttgattg aacatcattc ccatccaacg gctgatgaga
     2401 tttataaagt acttgaacat cggtttccaa atatgagtgt tgcgactgtt tacaataacc
     2461 ttcgcttatt tacttcgatt ggatttgttc aagaaatgaa gtacggcgat gcttctagcc
     2521 gatttgattt cagttcaaag cgtcattatc atgtaatttg tgaaaactgc ggtaagattg
     2581 ttgattttca ttacccaggg cttgaagata tagaaatggc tgcaagcaga ctgactgatt
     2641 ttgagattaa tgaacatcgg ttggaattgt atggactctg tccggattgt aaagagtcag
     2701 caaaaaaggc gcaaatgtaa aaacagtttc tacaagtgtt atacctaaaa aaagctagct
     2761 gtgtaaaaaa aacgctagct tttttatttt tttgtctttt attcacaatc ttagatataa
     2821 aagtgaatat tattaaaata agaatcttgt agatattggg ttcttatgct tgaaaaggaa
     2881 tgaaactaca aacaaacctt tgtgaattca caagcattaa actgttgtat ccgttttatt
     2941 tctgttatag tagttttgtc gaaggacaaa atgttaatcg gagggaccat aaatgaaagt
     3001 agtagtaatt ggctgtactc atgcaggtac tgcagctgta aaaagtattt taaaaaatca
     3061 tccagaagca gaagtgacag tatatgaaag aaatgacaat atttctttct tatcttgcgg
     3121 tatcgcactt tatgtcggcg gagtagtaaa ggacccagca ggcttgttct actcaaatcc
     3181 tgaagaatta tcttcattag gagcaacagt taaaatggaa cacgatgtaa cagacgtcga
     3241 tacagaaaat aaaacagtaa cggcaaaaga tctgaaaaca ggagaaacag aaacggtttc
     3301 ttatgataaa ttagtgatga caactggttc atggcctatt atcccaccga ttagtggaat
     3361 cgatgcgaaa aatgtactcc tatgcaaaaa ctacaatcaa gcaaatgaaa ttattgcaca
     3421 agcgaaagaa gcaaaacgtg tagtaattgt tggtggtgga tatatcggta ttgaattagt
     3481 agaagctttt gtcgaatctg gaaaacaagt gacattgatt gatggattag atcgcatctt
     3541 gaataaatat ctagataaac cattcacaga tatcttagaa aaagaattga cagaccgcgg
     3601 tgttactctt gcgctaggcg aaaatgttac tgaatttatc acagatgaag aaggaaaagt
     3661 gaaacaagta gctacaccaa cagatacatt tgatacagat atggtcattt tatgtgtggg
     3721 cttccgtcca aatacaaaac tgatcgaagg aaaagtagat acactgccaa acggggcaat
     3781 caaagtgaat gaatacatgc agacaagtaa tccagacatt tttgcagctg gagactctgc
     3841 agtagtcaac tacaatccaa gtggcacgca aaactatatt cctttggcaa caaatgccgt
     3901 tcgtcaaggt ttgcttgtgg gaaataactt gacagatcat aaaatggcgt atcgtggtac
     3961 tcaaggaact tcaggacttt acctatttgg ctggacgatc ggttcgacag gtgtgaccaa
     4021 agaaagcgca actttgaacg atctagaagt ccaagcgact gtatttgaag ataattatcg
     4081 tccagagttc atgccaacta ctgaaaaagt aatgatggag ctagtctatg aaaaaggaac
     4141 gaatcgtatc gtaggtgctc agttcatgtc caaatacgac attacacaat cagcaaatac
     4201 catgtcttta gctgttcaaa ataaaatgac tgtagaagat ctcgcacttt cagatttctt
     4261 cttccaacca cattttgacc gcccatggaa ttacttgaac ctacttgcac aagcagcgct
     4321 aggcgaaatc gaaactgcac aataaagaaa aagggttgtg acacaatttt gtcacgaccc
     4381 tctgtctttt tttgtgaaaa gtacaattag ttgtctttct tcgaattttt tgtcacaata
     4441 agcagcaaag ataaagctgt tacataaaag aacaagcagc tttatttgat tttgaaaaga
     4501 aaggtgagaa agaagatgaa taaagaatat tttgtccaac aagatgcatt tttagaagat
     4561 tataaaagtg cttcgaagaa attaacgatc aatattgatg agttggcaga tcaagtactc
     4621 tctcagttga atgaaacgca agaggaatat tacacgatgc cagcaaatga cgccaaagat
     4681 aatgtttctc atcgttttcc tttcagtaga cgcatctact cagatgaaaa tgacggcaca
     4741 ttgaatacag agtacacata tgaaggtcag ccatatgtgc tagaaactga tgatgatctt
     4801 taaagacagt ttttgaaaag gccgctcatc cttaagtgat gacaaaaaat catcacaatt
     4861 accgatgact gtctctttac tatcttttaa tcagaaaaag gggttatgac aagctttgtc
     4921 aacccctttt tttcttggtt tcagattggg ttttcctaca aaatattttt atctttgttg
     4981 aataggaaac aaaaaaacaa tataatgaaa gaaaaagaag gtgtagaaat ggataaaaga
     5041 caactgctaa tagaagaata tctggattta gctaatgaga ttattcatcg taataaacca
     5101 gaaatggaaa aagaatttgc aggatttact ctaaatgaac tagaagtgat tgaacatatc
     5161 gggaaaatcc ctgatcctaa cgtgacaaaa ttagcgaatg ctagttacat gactagagga
     5221 gccatcagta agctaacaaa aaaactgata gctagaggga tcataaacac ttatcaaagc
     5281 gaagagaata aaaaagaaat ctattttcaa ttgacgccaa tcggacaaaa aataaggaat
     5341 agacacgagc aactccatca aaaatgggta cagcgagatg ctactgtatt tgaaaatatg
     5401 tcaaaagaag aatttgacac agtatttcgt tttatcggtc gatacagaga atttatacga
     5461 agtagcaaat gaagctacta ttttttattg tagtgataat aggcaaaaat cataagcaaa
     5521 aatccgaata ccgtcggaag aaatggtgct gcagcataaa gctgtcctgc gattagcggg
     5581 ccgatgacgc gcgatagaga ttgtatactt tgggtaccgc ccattacaag accttgctgg
     5641 ttatctgaga cagaattgga tagttggcca ttgaatacag gagtaaaaat agaatcaccg
     5701 aaaccataac agataatccc aaataaaaac aaaatcggca gtagaagaat tccagataat
     5761 gtgatgaata gataggcgag gatttcactg aacatcccta ttcttgtgat ctgctgttct
     5821 gaaaattttt tgagcaatcg aggcataatg aacaattgcg agacagtatc caagattcca
     5881 atcaaagaga agcttaaacc aaccaaccca gccttccact gaaaggcgtc aatcgaaaat
     5941 tgagaaaaga tagattgaag agagccagct gccaaccaaa cagcaaaacc tgctgtcaat
     6001 aaaggaataa cggatgaaat cttgaataat tgttgtaatt ggtgaaaagg tcttacatgg
     6061 gagaaactta aatctactga acgttttttt attggaaggc tttctagcat aaatgtgtag
     6121 ccataaacgg catttaagaa agtaaagagt gctccaataa atataggaac agaattcccc
     6181 aactcagcta atagaccccc tataatagga cccagcgttg ttcctatacc gactaaagca
     6241 cccatccatc caaaaacttt ggttctttca ttaggttccg tgatgtcggc aaagtaagca
     6301 tataaggtgg agatttctcc tgccgtcaat ccttcaatga tacgaccaag gaacaacatc
     6361 catatcgaat ttcctaaacc aaaaagcaaa tatccgatac ttgatcctaa taaactgata
     6421 ataaggatcg gatgacgccc aaaatgatca cttaagctac cgagaatggg ggcagataag
     6481 aaggcagcta gagcataaac agacatgagc aaagtgatat agaaagcttg ctgatgtatg
     6541 gagtgagaat aaggcagcgt taaaaacgga agtacaggac tgacgatagt aaatcctaat
     6601 ccagtgaata agacagagac gtatccaaaa actttagaat gtttatttgt gaaactgtac
     6661 atgtaaaaac tcctttatgc aataatgtta acttggaaac aaaaatagtg tatagtaatt
     6721 ttgtttcctt gtcaacattc taaggaacag cgaaatctac ttaatttatt tattccgaaa
     6781 agtgaagata cgcatcaaaa acatgaacaa acaaaaatca agtagacgac attgtaaatc
     6841 tgaccgatca gtgaagtaaa tagaaaagaa acctgttctt gcttcccatg ttttttttac
     6901 gtatactgtg attgagcgga ggagaaatca tgttgaattt agaaaattac ttaagcgctg
     6961 gacaaacagt catatctaat cttttactta aaaattatca taggatcggt ctacaatctg
     7021 aagaattttt atttgttcta cagttgcata tggcacagct tgaaggagat actttccctg
     7081 atttgcagat gattgctatg gatatgggaa tcaaacaaga tcagattttt caaatactgg
     7141 atcgtctcgt aacaaatgga tttatcaaga tagaaacgac gattgtcaac ggtaaaaaag
     7201 cagatcgata taatctttac cccatctatg atctattagg tgagtattta aaaacacaag
     7261 aaaaaaaata cgaagaaaaa acacaagaaa aagaaatcca gtcagtgtat caattatttg
     7321 aacaagaatt tggacgcccg ctttcttcga tcgagttcca gcggatcgga caatggctag
     7381 aagaagacca ttatcagccg gaaattttga agctagcact gcgggaagcc gtattaaatc
     7441 aggcttatag ttttaattac gttgaccgca ttttgctatc gtgggaaaga aaaaatctac
     7501 gtacaaaaca acaagtggaa gaagaccaaa aacgaagaaa acagcagctt cttcaaagag
     7561 aagtagaaca gtcagctcaa caagaagaat tgccgaaagt ctcactgaaa aattggttgg
     7621 aggagtagcg gaatatgtta agcaaacaaa aaacaatgga agctctagaa acgatgtatg
     7681 gaatgtttcc cgaagcacat ggagaactca agcacaataa tccatttgag ttattgattg
     7741 cagtgatctt aagtgcccag gcgacagatg tatcagtgaa taaagcaacg ccagatttgt
     7801 ttgcctcttt tccgacaccc gatgcgttag cagaagcttc aatcgatgaa atcattctta
     7861 aaataaaaac gattggtttg tatcgaaata aagcaaaaaa catcaaagcg tgtgcacagc
     7921 agctgattga acgtttcgat gggcaagtgc caacgtcaag agaagaattg atgtctcttc
     7981 ctggtgtagg cagaaagaca gcaaatgtcg tattggggga tgcattcggg ataccagcaa
     8041 tcgcagtaga tacccacgtg gaacgtgtct caaaacgcct gaggatctgc aaactagatg
     8101 ctacggtcat ggaagtagaa gaaacactga tgcggaaagt accacaagaa ttatgggtaa
     8161 agacacatca tacactgatt ttttttggta gatatcattg cacggcaaga aatcccaagt
     8221 gcgaggtatg tcctttgcta tcaatatgtc aagatgggaa aaatcggatg cgtctaaaag
     8281 aaaaggcgct aaaaaagaaa ccacgcctgt agacgtggtt tctttaattg caaataaatg
     8341 tatgaattat cttgttatta tggagaaaag ctgtttcgtt tatctttttt gcatgaaaaa
     8401 gtaaatgtac atactcagta gctaggatgg taacttatta caatttattg cgataaaaat
     8461 aaaagttgtt atttttattg ggaaaatacc cgatgtagaa aataatgcct tccaatatac
     8521 ctataaaata agtccagtat tccaaaggat tatataaata agtgacgtat ctatctaaag
     8581 gtgtaataaa aatgataagt gataataaaa aaataccatt tataactaaa cataaactaa
     8641 tggatttggg cggtttcagg ctcatcagaa aaatatagat tctggtgaac aacaaccata
     8701 aaaaagtgag aatcattgca tgagagcttc tttccataaa ttttccttta agagtagtgc
     8761 ttaaaagtac agaaaaaata ataaatgcaa ggataggagc aattaaagta aatttattta
     8821 ataataaaca aaatttttca gcttttttat tcttggaata acgaggtatt gttttcgata
     8881 aaaaaagatg gtgcagacaa attatgataa ttaagcatac tatcagataa ctcaaaatga
     8941 agccttcttt caaaaaatta ttcaaaacca tatattgtat tttttatttg ataataacaa
     9001 tgggattatt aagaagtggc actaaataaa agactagtgt gtatttagcc gatttaatta
     9061 taaacaagta aatggataaa gtatatgtaa tgataaaaga aaaatggatt ttctaatttt
     9121 taagctaaat gttagatcac acatgtatat gctggatagt cttataaaaa atcatgtaat
     9181 acagcttggt acatgatttt tatttgtagc tgttttttac atagacacaa aaaagaaacc
     9241 acgcttgtag acgtggtttc ttttttacta tctgtttcca gaagctgagg tgctgctgga
     9301 tggtgtatgg tcatttgcag catccgctcc gctagttgct ggctcactgg aagaagcagg
     9361 aggagcagat gaagacggtg tcgagctttc ttcggatgaa gaagaaggaa ccgttgttga
     9421 agacgctggc tcgcttgttt ctgctgtcgt gctttctttt gaggactcag cagttgattc
     9481 gctttgacta gtagagctag agctttgagt cgttgtctct gttgttgagc ttccaggagt
     9541 ctggacatag ctggaagatg gaatagttgt tgatggtgta attgcattcg atctagctgt
     9601 atattgatct ttgtagtaca gttcaccgcc cacacgaatt aatccgcttg gcatctccca
     9661 gtctgtattt gtcacgttag ctgaaacata ctgcataagc tcgcgataaa catcagaggc
     9721 tacgtgtgac gattctgaag tgacaggtgt cattttttta ttgtaacctg tccacacgga
     9781 aatcgaataa tttggcgtat agcccgcaaa taggatatct ggatacaccc cagaagaaat
     9841 accaagtttt gcatactcgt catctgtgta attggatgtt ccggtcttac cggcttgata
     9901 taacccagcg atttgcgcat tcgtaccagt tccttcagtg atcgtatctt ttaaaatgtc
     9961 tgtgatcata taagctgttt ctggagacat ggctgttttt ccatctggct cataagtttc
    10021 ttctgttccg tcttggaaga cgattttatt aacatattga ggtttgtaat aagtccctcc
    10081 attggcgaaa gccgcatagg ctgctgccat ttttaaggaa gaagctccgt attttgtccc
    10141 atcttgttct tccgtattac tagagatcgc attcgattga tggatcgtac tgtattcgat
    10201 cccaagattt tttaagaaag aagccacttt atctgaaccg acttcattga ataatttgac
    10261 tgctggaaca ttacgggata agtaaagtgc ttggcgtaaa gtaatggtgc ccatatattg
    10321 attatcccag tttcctacag gagtcgaagt cccttcatag ttatacggtg cgtcagtaat
    10381 agtttttcca gtagaatatt tcaaatattc aaaagctggt ccgtaatctg tgacaggttt
    10441 catcgtagaa ccaaaatcac gggaagtatt cactgctaag ttgttgccta aagttacgtc
    10501 ttctgcgatg tgacgtccgc caatctgggc ttttactttc ccggtattgg tgtcgataag
    10561 ggtggaggca acttgcatct cgtcgtctgg gtaagagaca tactgatccg tgttgacgat
    10621 atcatatagt ttcttttggg cgtccagatc caaattcgta taaatctcta aaccgtctgt
    10681 gtagacattc ttatctgttt tttcttgtac ttcattgatg acttctttga cataattatc
    10741 gacgatctta gtattgtcat cggattgagt tagttcttgc agaccatccg ttaccggtac
    10801 attaacagct tgatcatatt ctgtttgaga gattttttcg ttttgaagca tcgtatacaa
    10861 aacagtatct cgtcttttct tcgcctgatc tggataaaca taaggatcat aggcggaagg
    10921 ggcttgaggc atcccagcaa gcaatgctgt ttgcggtaaa gaaagttctg ataatttctt
    10981 accaaagtac atctcagaag ctgtctccat cccgtacaag ccgtttgaca tatacacttt
    11041 atttacgtag taggtcaaga tctcttgttt cgattttttt tgttccaatc ttacagccat
    11101 ccacgcttct tgggccttgc gtttcagagt ctgatcctcg gcactggtgg agaagaagga
    11161 aagtttgatc aattgctgtg tcaaagtact tccgccttgc agtccgccag aggtaaagtt
    11221 agagagggca gaaccgatga tccgaatagg gtcaaccccg atgtgtttat aaaaacggcg
    11281 atcttctacc gacacgatcg catcttctaa tgttttcggt aattcattcg cagaaatttt
    11341 ttcacgcttt tctgcgccta aatcttcgaa tagttcgcca tcttgtgtat atagtttgga
    11401 agatacggta gcgtctaatt ttttatccgt caattcgggc gcatcttttg cgtaatacca
    11461 gaaaagtcct acacctgcta aaaagaggat acagaaaaat gagagtattc ctaaaaggat
    11521 cttgataaat aatccttttt tatgtgttcc agaggacttt ccagaacctt tgccagaatt
    11581 cttcttcaca ctctttttag gagtaggctg tttctgtcgt ctagagcttc tagtttgttc
    11641 atttgccatt cttgctttct ccttcaatag ataatgttga taaatagaag tcaaccgcgt
    11701 ccaaataagg aatccgcgga gaaataccag ttttgatctc tattcctagt tcttcgatca
    11761 aagataaagg tagtgatttg cgtccttttt ccttttgggc gtcccagtaa gcaatcaaat
    11821 gaacactatt caaataaaaa cagcgcttca aagacgaaaa ccataacaag acaaagcaga
    11881 ccccattttg ttcgaggcat gctttcatat gttcgatttg atgggcatgg aagtttttaa
    11941 agggaaaaga tgttttgttc ttggtttctt ttgcttcaaa gtccaagtaa aagcctcgat
    12001 agacgccatt atagtctgta gttgaggctt gggtaaagta ggcttccttg atcacagcag
    12061 cacttctctt aggatagtct actttcacga tttggatagg ggtaggcttt ttatggataa
    12121 ccgccaaccc gcgggaaaga taatatttat tactttcatt gattgcttct tcaaagcgca
    12181 tcccccgatt gccgaattca gtgatctgat ttcttttttc ttgaattttt tcgacgggag
    12241 aggcattctt ataataaggt tggccatttg gatagcgcaa taccatcttt aatcacactc
    12301 ctaggccaca ttatagcaaa aaagcggata gaaagaaaag gaatctgaga tggataagtc
    12361 aaaagtaatc tatgtgtctg ggtatcgaag tttcgaactc ggtgttttta aagaaaatga
    12421 tcctaaaatc caagttgtta aaaatgtttt aaaaaaagaa ctccatcagt tagcagaaga
    12481 agggctggaa tgggtacttg tcagtgggaa tttgggcgtc gaactatggg ctgtgcaaat
    12541 agtagcggaa ttgaaagaag aatatccgga actatctctt ggtattttgt atcctttttc
    12601 tgattttggt aataactgga atgaagggaa ccaagaaaaa aaacaattag cagaacaatt
    12661 agcagattat gtcgaagctg ttagccatca accctaccaa tcacctagtc agctaaagaa
    12721 tcacactcgt tttatacttg agcatacagt gggctgtctg ctgatctatg atgaagaatt
    12781 ccctggaaaa acgaagtatt tttttgaaga tgcacagaat taccaagcag atcatcccta
    12841 cgaaattcgg ctgatcagta tggacgatct tcaaaatttt agtgaataat cgatattgtg
    12901 tttgatttat caactttttt cctatacaat gaaaaaggta gaaatgttat tacgtaaatg
    12961 aaaactagag gtgtaaatat ggcgaatttg gtttatagtc ctaaagacat tttacaacaa
    13021 gaattcaaaa caaaaatgcg cggctatgat cccgttgaag ttgatgaatt cttagacaat
    13081 atcatcaaag attatgaaac ttacagcaaa gaactccttg ctttacaaga agaaaacgat
    13141 cgattgagtg caaaagtagc tcaattatct aaaactcaag gagcagctca aacacgcgtg
    13201 caacaaacag aggcaccaaa gagcgcagca gtgaccaatt ttgatatctt aaaacgcttg
    13261 tcaaatcttg aacgtgaagt atttggtaaa aagcttgatc aacaagcaag tgcagtcaaa
    13321 ccagcacaac ctaatccaaa taactacaca aatgcagaca caagcttaga tgataatgaa
    13381 aaaacacgtc aattttaatt tttgattaat gaatttgcgg ttttcgggta atcgcggtct
    13441 gacttgatca ggctgaggaa agtccatgct cgcacaggct gtgatgcctg tagtgttcgt
    13501 gcttagcgaa accataagct aaggtactct ttggagtaac ggcaggaaaa caagctaagg
    13561 cttcggctat gcttaagtat ccttgaaagt gccacagtga cgaagcggct ggggaaaccc
    13621 atccggtgga acgcggtaaa cccctcgagc gagcaaccca aacaatggta ggggcgcttt
    13681 ttgtctggaa gaagaacagg acaaaaaggc agtataactg cagatagatg gttaccgctt
    13741 tgttttttct ccctggaaaa gcaaagtaca gaacatggct tatagaaaac cgcaaacatc
    13801 agggaattag gtcgtgacaa aagtaatgcg ttataagaaa caagaaaaac atttgaaagc
    13861 tatttttcaa tacttcagat gaatcgttct tattacaaaa caaacttttg tatcaccggt
    13921 tattcggaat ttgagtaaaa gtgtggggta ggactgatgt cccacctcct ttttttataa
    13981 ataaggagat tgtgtatgga aaaaaacagg aagttcaacc tcgtcgcaac tgcggcaagc
    14041 gggctagaag cactggttgg taaagaatta agagatcttg gaatcgaatg ccaagttgag
    14101 aacggacgtg cacgttttga aggaacaatg gaaacgatcg ctacagctaa tttatggtta
    14161 cgtacagcag atcgagtaaa aatcgtggtt ggggaattcg atgcgtatac gtttgatgaa
    14221 ttgtttgaaa acgtaaaagc attgccttgg gaagattttc ttccactaga tgcagctttt
    14281 cctgtagctg gaaaatcgat caagtcgaaa ctatatagca cacctgattg ccaagcgatc
    14341 acaaaaaaag caatcgttga acgtctccgt acctattatc accgtccagc ttctgtacca
    14401 ctgacggaaa ctggcgcaca ttttcagttg gaagtagctt tgctgaaaga ccatgtgatg
    14461 gtaactctgg atacgacagg accaagttta ttcaaacgtg gttatcgctt agaaaaaggt
    14521 ggagcaccat tgaaagaaaa catggcagca gcacttatta tgttgacaaa ttggcgaaaa
    14581 gaccgtccat tttatgatcc tgtatgtggt tcaggtacgc tttgtattga agcagcctta
    14641 ataggccata atatcgcacc aggattcaat cgtgagtttg cttgcgaatc ttgggattgg
    14701 ttcagtcaag aaatcatgga aaatgttcga tcagcagccg aagaaaaagc agattatgat
    14761 attgaattag atattactgg ttcagatatc aatggacgga tgattgaaat tgccaaagcc
    14821 aatgcagaag aaatcggatt aggctcttcg attaccttta agcaacaagc agtcaaagac
    14881 ttcaaaacgg ataaagaata cggggtcatc gtggctaatc cgccttacgg agaacgtcta
    14941 ggagaagaag aaactgttcg gaaattatat aaagaaatgg gcgatgtatt ccgtccatta
    15001 aaaacatgga gtaaatatat cttgactagc gacttagcgt ttgaggaatt ttacggacaa
    15061 aaagcaacca aaaaaagaaa attgtacaat ggtgcattgc gtacggatct attccagtat
    15121 tggggaactc gtccgcctag aaaaaaagag aaagacgaat aatcttacta attgattcat
    15181 tcaattggat gtttggagga atagggaatg aaagaaaaag agtttttaca agaagtaaaa
    15241 gaaatcaatc ttctacaaca agctttagga atcctcgatt gggatacaca aacaggtatg
    15301 cctgaaaaag ctagtgcata ccgaagtgaa gtagacagct atctttacag catttatttt
    15361 tcaaaaaaaa tcggtccaaa aatcaaagaa gccattcgct atttttctga acatccggaa
    15421 gaattgtcgg aagttggaac tgccgttttt gccaaagtaa aagaagagta tgatttggaa
    15481 catcgcgtac cggaagatct tatgagaaaa tatacagctg ccgtttcttc agcacacgga
    15541 caatggcaaa aagctagaga aacacaacaa ttttctgatt ttcaacacgc attgaatcaa
    15601 aatattgaat taacaaaaca gttgatacct tactggagaa aagatgaaaa gacgaattat
    15661 gatgttttgc tgaatcaata cgaacccggg atgactgtcg aaatccttga taaggtattt
    15721 gatcagctta aagaaggaat tctttcgatc cgccgtacgc tccaagaaaa agggactgaa
    15781 cctgaaacag actttttaaa tcggagaatg acgaaaaaac aacagaagag atttgtagtg
    15841 aaagtaatcg aacaacttgg ttatgatttt agtcgtggtc gtttagatga cactgtccac
    15901 ccatttatga ctggaattaa tccgaatgat gcacgaatca cgacacgatg gaacgaaaca
    15961 gatttcaaaa tggctgtttt tggagtgatc cacgaggctg gtcatggtat gtatgaacaa
    16021 gacatcgatg ataaatatgc gtatacaccg gtttatgaag gtacatcaat gggtatccat
    16081 gaatcacaat cgttgtttaa cgagatcatt attggaagta gtcgcagctt ctggaaaaag
    16141 caatatcctt tctttcaaga atgtgcagaa ggaacattcg atgatattcc ttttgacaaa
    16201 ttttatcgtt cattgaacca tacaaaatca agtttggttc gtatcgaggc agacagtttg
    16261 acttaccctc tccatatcat cattcgttat gaaatcgaaa aaatgatttt caatgaaggg
    16321 gtagatgttg ctgaactacc tgaaatatgg aatcaaaaat atgaagagta tctgggaata
    16381 cgaccaacaa atgatgtgga aggcatccta caagacgttc attggtcagg tgcaagcttt
    16441 ggttattttc cctcttatgc attagggtac atgtatgcgg cacaattact tcattctttg
    16501 cagcaagaaa tagatgtgga aaatgttcta gcgtcagacg attattctcc aatcaaaaac
    16561 tggatgtcag agcatatcca tcaatatggt gcatctagaa aaccaaatca attaatcatg
    16621 gatgcaacac atgaaccgct taatccgcaa tacctgatcg actatatgaa agacctgtat
    16681 tttaatgtat atcaggtaaa ataaaataca atttacaaag atagctgaaa ttcatctaat
    16741 ctatagatgt tttcagctat ttacttaaga aagaagtgga acgaatggat aaattacaag
    16801 tgatcgctga ttatagtatg gaccaattag cgcatgatca aacaggacat gggagcgatc
    16861 ataccaaaag agttgtcaag ttagctgaaa aaattttgga gacggaaccg caggcagacc
    16921 gctttgtgac gcttgcagca gcttacctac atgatacgat tgatgataaa gtcgttaaaa
    16981 atgagaacga ggcaaagcaa cagcttcgtg tattccttcg ctctttgcca atagtagagg
    17041 aacagatctc gatgattttt tccattatcg aaaatatgtc cttttctaaa aatctctctg
    17101 aagcggccga actatctctg gaaggtaaga tcgttcaaga cgctgatcgt atcgaagcat
    17161 taggagcaat cggcattttg cgtactgctt atttcggcgg tggacatggg cacccgattt
    17221 ttgatcctga actgtatccg caaacattca aggacaaaga aaattaccga aaaggcacga
    17281 cagtgatcaa tcatttttat gaaaaactgt tcttgctgtc tgataaaatg aatactgatt
    17341 acggatatga agaagccaaa cgaagagaaa ccttcatgcg tgaattctta gaagaatttt
    17401 ttctggaatg gtctattgac gatgaaaatt ttactccaga caattggatc aaaaataaca
    17461 aaaaatgatg gaggttgtga cagaagtggt cagctccaag atatttcgga tcattttccg
    17521 taaaagttac ttctggaaca ctgtttacca agaggtcgtg agcctgccgt tcagctccga
    17581 gtcacaagga acaatttcaa aaaatagctt ctcgtgtttg atgaatattg taacttgtga
    17641 ttcggaattg gcaggagaac accgtttatt cggaagaata ggactgtgac aagacttttg
    17701 tcacagtcct tttttcgaat tcaacaagta aaacttttaa gaaatattca gagagtaaaa
    17761 ttaaaaattg ctgataaacg aagatggaaa ttttatgtat ttttattggt ctatgcaata
    17821 aataaattaa atattcagaa tacaattgat tttaacgaaa gggcttctat caatggttgt
    17881 gttgtattta tctgcccagg tagagtaaaa tagatcatta tctaaaaggt aattgttcat
    17941 actatataag tcatttgaaa gtaaaaagat actgtaaata aaaaatgtgg ttaaataatt
    18001 ctcataatca gtgtaagcgt tatcaaaaat atagcatact tgctttgtag atgaatgaca
    18061 cgatatatgt ttggtatgtt ctgcaagaca gatgaatatg gaggtggaga caatgtcttc
    18121 caaagaacta acaatgtatt tagagaaaga attggatcaa ctgaaagaaa aggggttata
    18181 caatacgatc gacatactag aaagtgaaaa tggtgcttgt atcatcgtag acggaaaaaa
    18241 gatgatcaat ctagcatcca acaattatct tggatttgcc aatcgcgaag aattgaaaaa
    18301 agcatgtatt gaagcaaccg aaacttatgg tgtaggagca ggagcagtcc gaacgatcaa
    18361 tggttcgctg aaaatccatc agcaattaga agaaaaaatc gcagaattca aagggacaga
    18421 agcagcaatc gcctttcaat ctggattcaa ctgcaatatg ggcgctatct ctgcagtaat
    18481 gacaaaagaa gatgctatct tatcggatga attaaaccat gcatctatta ttgacggttg
    18541 ccgcttatcc ggagcaaaaa tcatccgaat taaacatcaa gatatgaaag accttgagaa
    18601 aaaagcaaag gaagcgatag aaagtaagaa atataaaaaa atcatgtata tcacagatgg
    18661 tgttttctcc atggatggag acattgcacg attgccggaa attattccga ttgttgaaaa
    18721 atatggactg atcacctacg tcgatgatgc gcacggctca ggcgtaactg gaaaaggtgc
    18781 aggaaccgtc aagcattttg gattaagcga caaaatcgat atgcaaatgg gcacgttatc
    18841 gaaagctata ggtgttgttg ggggttatgt agctggatcg aaaacactaa tcgactggtt
    18901 gaaagctaga agtcggccat tcctattttc tacttcgcta actccaggtg cagctgcagc
    18961 tgcattggct tcgattactt tgatgcaaga acatccggaa cttgtcgaaa aagtttggga
    19021 aaatgctaat tattttaaag aagaattgaa aaaagtaggc tataacatcg gtatgtctga
    19081 aacacctatt actccagtca ttttgggaga cgaaaaagtg actcagactt tctctaagaa
    19141 gctgatcgaa catgggattt atgctaaacc aatcgtttat ccaactgtgc ctcttgggac
    19201 aggcaggatc agaaatatgc cgactgcgga acatacgagg gaaatgcttg atgaagcagt
    19261 agcagtttat caaaaaatag gaaaagaaat ggaaattatt taactaaatt tagttttttc
    19321 gtctatttaa aattagagaa catgttcacg catatattgt taatcatctg aagatagtag
    19381 taaaaaagat cagggaggat catcatgaaa aaagtattgg tgacaggatg tttaggacaa
    19441 attggttcag agttgatcac acggctacgt caagaactag gcagtggaaa tgtcattgcg
    19501 acagatatac gcatgcctga agtcattgag aaaaacgata tctttgagat cctagatgta
    19561 atggattacg aaaaaatgcg cagcatagca gaacaatatc aagtggacac attgattcat
    19621 ttggctgctc ttttgtcagc tgttgcagaa gcaaaaccac aattagcctg gaatttgaac
    19681 gtgactgggc tggtcaatgc attggaagtc gctagagaat tccagctgaa attttttacg
    19741 ccaagttcaa tcggcgcgtt tggtccaaat actccaaaag atcatacccc acaagatact
    19801 cttcagcgtc ctacaaccat gtatggggta acgaaggtag ctggagaact actatgtgat
    19861 tattattatg tgaaatatgg cgtagatact cgtggtgtcc gtttccctgg attgatttct
    19921 tatgcaacct tgccaggagg cggtacgaca gactacgcag tcgatattta ttattcagca
    19981 atcaaagaag gaaagtacac tagtttcatt gatcaaggta catcaatgga tatgatgtat
    20041 atgccagatg cgatcgatgc cattataaaa ctcatgaatg cagatcctga tcgattggtt
    20101 catcgaaatg ccttcaatat atcggcgatg tcttttgacc cggaagagat caaagcatct
    20161 attcaaaaaa tcataccaga ttttgaaatg gattatgatg tggatcctgt acgtcagtca
    20221 atagcagaat cttggccaaa ttcactagat gtttcttgtg cgcaagagga atggggcttt
    20281 tcgccgaaat acgacttaga tgtgatgaca aaggatatgt tggaaaaact gaaagaaaaa
    20341 ttggtagaga cggtataaaa caagaaccac atccaaaaag ttttttatac ttttgggatt
    20401 ggctatggta tgaaagggga caagggatgg aagaatatca agccacgcca gtaaaagaag
    20461 taaaaataga aaatgaatta aaacgtacga tgggattttt tactgcttta tcgactgtta
    20521 tgggcacagt gatcggtgca ggggtatttt ttaaagccgc aagcgtagca gaagttacag
    20581 gatccgccag tttgcatatg ttttcttggt ttctcggtgg tatgatttct gtctgtgcag
    20641 gactaacagg agctgaactg gcagctgcta ttccagaaac aggagggatg attaaatata
    20701 tcgaacggat ttacggaaat acagcagctt ttttattggg ctgggcgcaa gtcgtgattt
    20761 attttccagc aaatgtagca gccttgtcga tcattttcgg tacacagttc gtcaatctat
    20821 ttggtctttc ccagtcaatg attgttcccg tagctgttac agctgctgta tcgatcctgt
    20881 tgatcaattt tttaggttct aaagctggag gggcttttca gtcgattacg cttgtctgca
    20941 agcttattcc tttgtttgtg atcgtcattt tcggtttgtt ccgacaagaa ggagtagact
    21001 tccaactgtt tccgatccaa gcaggagaaa atctttcatt tttctcagcc ttgggagcgg
    21061 gcttgctagc aacgatgttc gcatacgacg gttggataca cgtaggaaat atttcgggag
    21121 aattgaaaaa gccggcgaaa gatttgccaa aagcaatttc tttaggaatc atcggaatca
    21181 tgatcgttta tttattagta aatgcggtgt tcttaagaac cgcatctatc gacggagtcg
    21241 caggaaacag caatgcggca agtgacgttg caaaaatgat ttttggcggc ttcggtggaa
    21301 gactggtcac agttggtatc ttgatttctg tttatggaac catcaatggc tataccctta
    21361 ctggaatgcg tctcccttat gttatggcaa aagaaaacaa cctgcctttt agcaagctct
    21421 ttgctaaact tcatgataaa actaaagttc ctgtagccgc aggtatctta gagctagtca
    21481 tcgctattgg catgatgatg atcggtggtt ttgatacact aacggatatg ctgatctttg
    21541 tcatttggat tttttatacg atggtctttg ttggtgtcat tcttcttcgt aaaaaagaac
    21601 ctgatttgtt tcgaccatac aaagttccaa tgtatccgtt tattccacta gtagcaatca
    21661 ttggagggac ttttatcgta tcaagcacgc ttatcactca gaccttttta gcttcgatgg
    21721 ggatagtatt gacacttgct ggtatcccga tttatctcta tttaaaacgt agatagctaa
    21781 aacattcgaa ttttattaga actgcacttc tctgccaaac aaaatatttt atttaaatga
    21841 ggagtacgtg gaagaaatgt caaaagtact cgttaatttt gatcacgtaa atgtcgtttt
    21901 gtctacgatg aaatagcaaa gtaaaagaca gaaatcgggc tcaaccgatt tctgtctttt
    21961 actttgattt gcgtaactga actattcggc gtacgccgcg ataaatattt aataaaacca
    22021 agcaaccaga aatgaaaatg ggaagccaaa tcagatagac agggtagcca agcactttag
    22081 acaaagcatg tggagtcatt ttatccattt caagaagtgc ttgctgccaa gtctgaagat
    22141 tgaagactag catgaaaccc gaaataatca ggagaataaa tcctaaataa tgaattgtag
    22201 ttctttttct tacataagcg atcgtagcat aaaggaacag taaaaaaata ggtacgccaa
    22261 aaacaaagta catattcagc atctcgacac cccctttgtg tttatcttac tttactttga
    22321 aagaaatgag aaataatagc ctcgttactt gcttgaaaca gcaaaggact gctaagataa
    22381 gagagataca aagaatcaag gaggagtcat atggcaagtt atatcgaacc gattcgtttt
    22441 gcgctgattg tttttccatt tttagcctta gcgatctcat ctgttttttt tatttatgaa
    22501 tatcgaaagt atggaacatt tctagtaaca agggcagtta ttttgtattc tttcgttttt
    22561 tatttattgt gtgcgtattt tttggtgatc ttaccacttc cggcaaagga gacggttgcc
    22621 caaatgacag ggccaaagat ggagctgcat ttatttgctt catggcaaca tttcagagac
    22681 aacacggtat tggtcttgtc tgaacctagt acctacctgc cggcaatgaa gcagagtgta
    22741 tttttggaac cagcttttaa tgttttatta ctcataccat ttggcattta tttaagatat
    22801 tatttccggc tttcttttat gaaaacgatg attgctagtt tctgcctttc tctatttttt
    22861 gaattgacgc agctaagtgg actttatttt atctacccaa gaccataccg cttatttgat
    22921 gtgaatgatt tagtgacaaa tacattgggt ggaatcattg gttttgtcgt tacgccgtta
    22981 tttacattta tgcttcctac aagaagacga atggatgaac tttcttatga aaaaggaaag
    23041 actgtctcat tgactcgacg cttggtcgct tatttgatcg actggacatt tttatctctt
    23101 attaatttgg tagcggcgat tttctttaaa attgcgttcg attcaggggc agttacttct
    23161 catatctggt ggcttttatt agaagtaggg gtatacttta tgattgtacc gtttataacc
    23221 aatggatgga cacttggcaa aaaaattgtg cgtatccgtt tggtggaaga agaccgcgaa
    23281 cgaatttctt taaaagcatt gatgatccgc tacgggtatc tgtactttct tttttatggt
    23341 ttaagctggt tggggcagtt gcttcagcaa atcatgaatt cgacaacgaa tacggtgttg
    23401 cagatgagtg cagtattcat ttctctcggt gtcttgttac tacaaggtat ttttatttta
    23461 aatatcttgc tatcattttt gagaaaaaaa cggattctgt tttatgaaaa agctagtcac
    23521 acatatactg ttagcacgat tggccaaaca aaggatgatg attctcgaaa ttagctgagc
    23581 aaaaacgctt tgtctgtgtt ataatgccaa agtcgtgata atttaaagag aagaggtgca
    23641 cgaatgaata attttgatat attccgcttc aaaccgttta tcaatgaagc gttaaccgca
    23701 aagggcttca aacagcccac agaggtccag gaacgtttga ttcctttaat tgcaaaagga
    23761 aaaaatatca ttgggcaatc tcaaactggt acaggaaaaa cacatacgtt tttgttgcct
    23821 ttgtttgaaa aaatcaatgc agaaaaagag gaagtacaag ttgttattac agctccaagt
    23881 agggaactag ctgcacagat ctatgaagca gctacacaaa tcgctaaatt ttctacaccg
    23941 gagatccgtg tctcgaattt tgtcggcggt acagacaaac agcgtcaatt aaatcgtctg
    24001 catcaccaac agccacaggt tgtagtaggc acgcctggta gaatattaga catgatgaat
    24061 gaacaagcgc tgaagattca caccgcattt gcatttgttg tcgatgaagc ggatatgaca
    24121 ttggatatgg gttttttaaa tgaagtagac caaattgcaa gtcgattacc agaaaagctg
    24181 cagtttttag ttttttctgc cactattcca gaaaaacttc gtccattctt acgaaaatac
    24241 atggaaaatc caatcattga agaaatcaaa ccaaaagcag ttatttctga agacattgaa
    24301 aattggctga tttcaacaaa aggtaaagat gagaatcaaa tgatttatca actgctgaca
    24361 attggtcatc cttatttagc aatcgtattt gccaatacaa aacagcatgt ggatgaaatt
    24421 gccgattact tgaaaaacca aggcttgaaa gtagcaaaga ttcatggaga catcacccca
    24481 agagagagaa aacgagtgat gcgacaagta cagaatctgg agtatcaata tgttgtagcg
    24541 actgatttag ctgctagagg gatcgatatc gaaggtgtct ctcatgttat taatgcagaa
    24601 attccagaag atctagactt ctttatccat cgtgtcggac gtactggacg taatggctta
    24661 aaaggaacgg ccattacgtt atattcgcca aatgatgagc aagccattga tgcgatagaa
    24721 aaattgggcg tctcatttga accaaaagaa atcaaaaacg gggaaatcgt caaaacatac
    24781 gatcgtaata gacgtgccaa acgagaaaaa tcaaaagatg tattagatcc cacgctgatt
    24841 ggtcttgtga agaagaaaaa gaaaaagatc aagcctggtt ataagaaaaa aatcgattgg
    24901 gcaattgcag aaaaaaataa aaaagaacgc aaaatcgaac gcagacagca aagccgtact
    24961 gctcgtaaaa ataagaaaaa ttcacataaa taaaaaacaa gaggttgtga caaaaggcgt
    25021 gtcacaatct cttgttttta agctagactt tttttcattc cgatacaaag tacttcatct
    25081 tcaataaaag gttctgtgca agtatgatac ccttgttttt cataaaaatc tacagccgtc
    25141 atttcagcta ctacataact agtctgacaa ccttctgaaa ccgcccgttt ttcagcagct
    25201 tcaagcaact gaccgcctaa cccttttcct cggtattctt ttgctacgca aaatcgttcc
    25261 ggcttgaagc aagtaggtgt tttcttttca taacgcaaag tagctactgg tcttttttga
    25321 tcaagaaata gataataggt ttgttgcggg atatcttttc cgtcgaattc tagttctgca
    25381 ggaatccctt gttctaatac aaaaacttga tatctcaaaa agcagctagc tgcgcgtatc
    25441 cattcatccg ctccaactat ttttttcatc atattcctcc ttgtcatgaa tagcagagat
    25501 gtattgattc ctttattctt atcgtaccaa aaatcgtgga gtatgaccaa aaggaaatga
    25561 aaataaaaat tgagatattg acagtgatca atagaatgcg tatactaaat aagtacgaaa
    25621 aggatatgat ttgtttttta agactcacag aaaattcttt cttggctgaa aaaagaatag
    25681 tcactaatca aattgctccc tttttctatt ttggtagaaa taccgacgca gctcagcgtt
    25741 atcgagttta agaggtaatg attacacatc attgcaatca aggtggtacc gcgagttttc
    25801 gtccttggct gtgatggtgt ttttttgtat ttaatttata gaaagcaggt aatgacatga
    25861 aacaattaag tagtgcacaa gttcgacaaa tgtttcttga tttcttccaa tctaaagaac
    25921 atacgattga acctagtgcg tctttgatcc ccgtgaacga tccaacctta ttgtggatca
    25981 actctggtgt tgcaacattg aaaaaatatt ttgatggttc agttgtgcca gaaaatccaa
    26041 gaatcacaaa tgcccaaaaa tctattcgaa caaatgatat cgaaaatgtc ggtaaaacag
    26101 cacggcacca tacgatgttt gagatgctgg gtaatttttc gattggtgac tatttcaaaa
    26161 atgaagccat ccactgggca tgggaatttt tgactgatcc aaaatggatg gcattcgatc
    26221 cagaaaaact ttatgtaaca gtttatccaa aagatacaga agcaaaacgc atctggcatg
    26281 aagaagttgg cctgccacta gatcacatta ttgatgtaga agacaacttc tgggatatcg
    26341 gtgcaggccc atgtggtccg gatacggaaa ttttctatga ccgtggagaa gcttataacg
    26401 atttagcaga agacgatcct gaaaactatc caggaggaga aaatgaacgt tatttagaaa
    26461 tctggaactt agtattttca gaattcaacc ataagcctga taacacattt gaaccacttc
    26521 ctcataaaaa cattgatacg gggatgggat tggaacgcat ggtctcaatc gttcaagatg
    26581 cgtcaacgaa ctttgagaca gacttgttta tgcctattat ccacgccgta gaaaagctaa
    26641 gcaaacaagt aaaatatggt gaaaacccag taacagatat ttcttacaaa gtaatcgcag
    26701 atcatattcg cgccctttca tttgctattg gcgacggtgc attgccatcg aatgaaggga
    26761 gagggtatgt cttacgccgt ttgcttcgtc gtgctgtcat gcatgggaaa aaattaggta
    26821 ttgaacaagc attcttatat cgtttagtcc ctgttgttgg ggaaatcatg gtaagttatt
    26881 atcctgaagt acttcaacaa aaagaattta ttgaaaaagt tatccgtaca gaagaagaac
    26941 gtttcaatga aacaatcaat gaaggtttgg aaatcttaaa tcaagtgatc caagaagtaa
    27001 aaaacgaaaa cgggacgact ctagcaggaa aagacatctt caaactatac gacacatatg
    27061 gcttcccggt agaattaacg gaagaggtgg cagaagatga aggactgaaa gttgatcatg
    27121 ctggttttga aaaagaaatg gatgcacaac gcgaacgtgc tcgctctgcc cgcagtactg
    27181 aaaaatcaat gggggtacaa tctgctttat tgacagatat caaagtagaa agtaaattca
    27241 ttggatacac agaacaagaa tcagatagtc aattattggt catcgttaaa ggtgagtctc
    27301 ttttgaatga agtcgcagaa ggtgaagctc aaatgatctt tgatcaaaca cctttctatg
    27361 cagaaatggg tgggcaagta gcggatcaag gatggatcat gaatgaagta ggcgaaatcg
    27421 tagcagttgt tacagatgtt caaaaagcgc ctaatggtca atttatgcac accgtagaag
    27481 taaaagcacc aatgagtgaa ggacaaacct atcatctatt gatcgatcaa tcgatgcgca
    27541 atcggatcat caaaaaccat acagcaaccc atctactgca caaggcattg aaagaagtac
    27601 ttggcgagca tgcaaatcaa gctggttctt tagtcgctcc tggacattta cgattcgact
    27661 ttacccactt tggtcaaatc acttcagatg aattagctga aatggaaaga atcgtcaacg
    27721 aaaaaatctg ggaagctatc ccagttgaaa caattgaaac agatatcgat acagccaaaa
    27781 acatgggtgc aatggctttg tttggtgaaa aatacggaca tgacgttcga gtagtaaata
    27841 ttggcggctg gtcaatcgaa ctgtgcggag gcacacatgt agcaaataca gaaaacatcg
    27901 gaatcttcaa aatcgtttct gaatcaggaa ttggtgcagg cgtgcgacga atcgaggctg
    27961 taacaagtaa agaagcgtac gaactacttg aagaagaaga aaaacaacta aaacaagtag
    28021 caacaattgt taaatcacct caattgaaag aagttgtttc aaaagcggaa caattgcaac
    28081 agcaagtaag agagctacaa aaggaaaatg aacaattagc tagcaagtta gcgaatcaac
    28141 aagctggtga tgtattcaaa gatccagaaa cgatcaatgg aaaaacaatc attgcagcac
    28201 aagtaaaagt gaaagatatg aatcagctgc gtcaattagc tgaccaatgg aaacaaaaaa
    28261 atatttcaga tattcttgtt ttagcaaacg aacaagaagg aaaagtcagt ctgctagcgg
    28321 caatgacgaa agaatcaaat gaagctggac taaaagctgg cgacttgatt aaagcaatcg
    28381 caccgaaagt aggcggtggc ggtggtggac gtccagacat ggcacaagct ggcgggaaaa
    28441 atcctgctgg tattccagat gcacttaaag ctgcgaaaga atgggttgcg caacaatagg
    28501 gcgtttacgt ttgacagaaa aacaaaagtt caaacattga acgaaacatg attatggggg
    28561 cgaagacaaa caagtcttgc cctcatactt ttttcaattg gatggcgcat ctaatcaaat
    28621 ggtcggattc ggatggtctt tagttgacga aagtgacaga aatttttacg attagtataa
    28681 ataaaaaggg gtgaaaaaat gaatagtaga aataagacct ttcgactagt cttacgtgcg
    28741 atcttgttag caatcatcat cgtacaagcg atggttcctt ggctggggtt tattccatta
    28801 ggattcatta gtttaacaat tatccatatc acagtaatta ttgctgcagt agtattaggc
    28861 ccaaaagatg gcatggtgat tggcttgttt tggggaatcg ctacaattgt tcgtgcctat
    28921 gccatgccaa caacgccatt tgatacatta gtgttcacga atccgattat ctcggtagtt
    28981 cccagagtac ttgttggctt agtagcaggc ttggttttcc actggattta tcaaaaaaat
    29041 aaatcaataa cgatcagctc agcctttgca ggggtactgg gttcattggt caatacagtc
    29101 ttagtgttag gattcatggg attattttat acaggtgcaa cggcagatgc ctatggtgta
    29161 gactctgctc ttttatttaa aaccttagca ggggttgcag caatcaatgg gataccagaa
    29221 gcaataggtg cgggaatcat aacaccatta ctagcaaaag cgttatttgc agcaacaccg
    29281 ttgaaaccag aataatgacc atcttttaat agtaatagga cgcacgatag atagaaaaag
    29341 gaagagagga gaggaaaaca atcgtcctct ccatcttttt acctgagcaa caagcaaact
    29401 agaaggggta aaaaacttgg ctaagaaaca agaagaaaaa aagaaacaaa taccgcaaca
    29461 gcggacaaaa aaaggagtac tagcgggtat cttaggattg gttacgaatc tgatcttgtt
    29521 tgtctctaag ctaatgatcg ggcttttttc aggaagtgtc tccattatgg ctgatgcagt
    29581 gaacagtcta tcagatactg cttcatccat attgactttg atcggattta aaattgctgc
    29641 aaagccagca gatcaggaac atccttatgg acacgaacgc ttcgaatata tcagtgggtt
    29701 atttgtttcg atcatcatta cttatgtagg atttcaattt ttagagtctt ctgctgaaaa
    29761 aatcatccat ccagaaaatg tctctttaac acccatcgtc ttttttgtcc taatcatttc
    29821 catctttttg aaatttgcac aaggcaaaat gtatcaggtt attgcgcaat ccattgattc
    29881 tgaaacattg agagctacag caacagatag ttataatgat gtctttacta cactggctgt
    29941 attgctttca gcagcaatcg aatgggtaac tggatggcgt attgatggat atataggttt
    30001 tgctttagca ttatatatcc tttatagtgg attgacgatg cttcgaaatt ttgtatacga
    30061 actattagga agtcgcccaa cgccagcaga gattgaagaa atggaagagc atttaaatgc
    30121 ttgcgatgat atacttggct atcatgattt attggtgcat aactatggac ccaataaacg
    30181 ctttgcatca gtccacatcg aagtagatga atcaatgaat ttgaatgtgg ctcataagat
    30241 tattgatctg atagaaaaag actttaaaaa gtctttggat gtggaacttg tctgtcactt
    30301 ggatccaatg cctgttgata acgaaaaata ctataatatc ttgcggcagt taaagcaaat
    30361 tcttcttgaa acaggtacag ggcttaatat tcatgatttt agagtaattc atcaaggaaa
    30421 aacgctgcaa tttgatgtag tggtccctga gaaattccag tttgttgata cagaactaga
    30481 aagaatctta cgtagtcaaa tagaagaaaa aatcggagat taccaattgg aaattacgtt
    30541 tgatcacaat tatcttttat attaaatgag aaactataaa aagactcctt aaagttttta
    30601 atttaaagag tctttttttc ttttaattgt ttatggagag cctgacgtgc aagattatct
    30661 gctcctttat ttttgttttc gggcaaccat tgcaggatca ataaagaaaa ttcggataga
    30721 agttcttgga ttctttgaag gtagggaagg aagagctcgt tcttcgttgc gtttttgtcg
    30781 accgtagaaa ctagtgtttt gctgtctgaa aaacagaaaa tcgtttcatt ctgtaagtcg
    30841 tttttcttta gaaattctaa agtttttaaa aagacttcga actctgcttg atggtttgtc
    30901 cctatagaaa gcgggataga tagctgttct tgtttacctt gatagacaat caagattcca
    30961 cctccactag gaccagggtt gccttttgta gaggcatcaa tgtatacttt taacataaaa
    31021 agagattctc ctttttgttc aaactaatgt tatggtaaga ttatatcatt actgaatcgg
    31081 tgagaggtta gagaatgaaa aaaaatattc gatggcagcc tgaactggca catacgatca
    31141 tctattggtc attgaccttg atcattctgt tttttagtct gattctttct ttagaaaaca
    31201 caggtcctta ttggaaaagc aattttgttt taggtatttt tcttgttttt ctatttctag
    31261 gtttgcggcg ttcttttatt ttaaaatcaa actctgtgat tatacgttat gctgcttttt
    31321 ggaaagaccg agaaatatct gtcggacaga ttcgatcttt gacgccaact aatactgggg
    31381 tgcaaatgat gttgcaagat gagaaagccc catctgtttt catgatgcga aaaaaagatc
    31441 aagtagtcct ttttgatcat tttgaaaaaa acggacccaa atcagaatca attccttatc
    31501 cttatacgaa aaaaagaaaa agctaatttt aaaaatcaga acctaaaaaa acgaattttt
    31561 acaggaaaag aaaagagtga gacgtacggt tcatcccgtt tagcgtctca ctctatttgt
    31621 ttatctattc atgcatacta gtatcggtag tctgaacgtt gcctttcttg acatgggcag
    31681 cttgtgttcc tcgattccct tcgatgatct caaattcgac cacttgattt tcttccaaag
    31741 ttttgaatcc ctcttcttca atcgcagtaa aatgtacaaa aatttcttct gtgtcatcat
    31801 aactgataaa gccgtatcct tttttgttgt caaaccattt tacaattcct gtggtcaaag
    31861 cactcacgac ctttctaaag tctgatcata gccttcatta taaagagtat ttataagtac
    31921 gtcaaataaa ttaaaaacct gtaattttat gttttttttc tgaataaaaa aaggtataat
    31981 tggcatatct taacaaagaa gatattttgt tgaataaagt tcgtgaaaag gggtaatcaa
    32041 atgaagaatg atctgcaaat ttcacaagaa acagagctag aaccgattgt tgaagtggct
    32101 agtaaattag atcttaagga agacgatttg gaactgtatg ggaaatataa ggcgaaaatc
    32161 aatttttctg gaatcaatcg atcaaaggat aacgctgagg ggcatttgat cttagttact
    32221 tccatcaatc caacaccagc tggcgaagga aagtcaacga tcactgtagg gttaggagac
    32281 gcgctcaata aaatcaataa aaaatctgtt attgcattgc gagaaccctc acttggtcct
    32341 gtgatgggaa tcaaaggtgg tgctgcaggc ggaggatatg cacaggtatt gcctatggaa
    32401 gatatcaact tgcattttac tggtgacatg catgctatca caacagctaa taatgcatta
    32461 tctgctcttt tagataatca tattcatcaa ggaaatgaat tgaacatcga tgcccgccga
    32521 gtgatttgga aacgggtagt cgatctcaat gatcgtgaac tgcgtaaagt aattgttggc
    32581 ttgggtgggc caatccaagg tgttccaaga gaagatgggt ttgatatcac agttgccagc
    32641 gaaatcatgg ctatcttgtg tcttgcggca gaccttgaag atttaaaggc tcgtcttgca
    32701 aggatcgttg tcggctatac atttgaccgt cagcctgtga ctgctggaga cttgaaagca
    32761 gaaggcgctc tggcattact gctgaaagat gcaatcaagc cgaatcttgt acaaacaatt
    32821 tacggaacac cagcttttgt ccacggggga ccgtttgcca atatcgctca tggctgcaat
    32881 agtattttag ctaccaagac agcattgaga ttagcagatt acgttgtcac agaggcagga
    32941 tttggtgcag atcttggcgg agagaaattt ttagatatca aagtacctaa cttgaaaaaa
    33001 gcgccggatg ctgtggtgat tgtcgccact gtccgtgcat tgaaaatgca tggcggaatg
    33061 aaaaaagatg aacttaaaaa tgagaatctt gatgctttga aagtaggttt tgcaaattta
    33121 aagcgccata tacgtaatat ggaacaatat cagctacctg tgatcgtcgc aatcaatgaa
    33181 ttcgtaacag atacagacaa tgaactcaca ctgttggagc acttgtgtga aaatcagggg
    33241 attcttgcca agagagcgag tgtatgggca gatggagcag aaggcggcgt tgatttagca
    33301 aaagctgtag tacgattgat tgatagaaaa gaagctaatt acaagccatt ataccgccta
    33361 gaagaaacga tacaagaaaa gacagaaaca atcgtcaaga aaatttacgg aggaaatggt
    33421 gttgttttca gcaaaaaagc gcaaaaacaa atagaagagt ttacgaagaa tggatgggac
    33481 aaactgccga tttgtatggc aaaaacacag tattcatttt ctgatgatcc ttcactgcta
    33541 ggtgcaccgg aagatttcac catcacgatc cgcgaaatca tcccaaaatt aggggcagga
    33601 tttttagtag ccttgactgg agatgttatg acgatgcctg gtttgcctaa aaaacctgct
    33661 gcattgaata tggacgtgac aaatgacgga gaagtgttgg gattgtttta agcttgatta
    33721 taatctacca tcgaaggagc tgtattttga ctacagcttc tttttttaga cgatagggaa
    33781 ttaattttgt ttacactagg atcattcttt ttctttattg tattttgagc tatactaaag
    33841 cataatagga gggtatgatg aaaacgacat ttataagaaa aaccggtttt tacggtatgg
    33901 gtagctcgat tcagctaaga atcaacaatg aaaaacgcag tgttctcagt catgaacaaa
    33961 agataacgct tgatctggaa gtgcccttta cgatgcaggt caccttttat tggctaaaaa
    34021 gccccgttta tacagtgaaa gaagcaaagc ctgcttatgt gatcacaatg aattacttga
    34081 ttcttcaaat ctatccgttt ttatttctag caactgggtt gggggccgtt ttcctacaaa
    34141 atttattttt cagttttttt gcagttattg tgttgattgc gttctttttc tatataaaaa
    34201 accgggcata tttgatcaag gaggaaaatt atggcaaact ctgatctttt tttaatcagt
    34261 ttcaatcgaa tcgaaaaatg gatgagagac gagatgggaa atgcacgaaa tatgggattt
    34321 accgaacttg tcagacgcct agctcaaaga aaacaattaa tgatcagaaa atacgaagat
    34381 gatcttttac agcttgctca gttaaggaat gcgatcgtcc atgatcggat cgcagtcgat
    34441 tttgtcattg cagagccaaa tgattggaca gtcaagcgaa tccagctgat cgaacaagaa
    34501 ctgattcagc ctgaaaccgt gctgcctcga tttgcaaaac gtgtaacagg ttttgaacaa
    34561 gatcttccgt tacttgaact gttaaagata gtagcagaaa aaaggtattc acaatttccg
    34621 ttatacaata aaggaaaatt tgtagcattg attactttac gtaatatcgg gttttggcta
    34681 gcaaaagaaa gccaaaaagg tccggttgat ttaacaaata aacgagcgtt ggatttgatc
    34741 atccaaaacg gaaagtacac aaattatcat tttgttccag ctaaaacaca tatttacgag
    34801 gttgaagcga tgtttcgtga gcaagggact ttggaagccg tacttatcac taaagatggg
    34861 aatcccgatg gtaatctgct cgggatcgta cgacctcgtg atatttataa acaaatagaa
    34921 aaggattgat tttttgttaa ttatttattg gatattcagc gctttgctag taggagtgga
    34981 tcagttggtc aaatggtgga tcactgataa tctagcgctt ggagagacga aaaatcttat
    35041 accaggtatt ttgtcactga atcatattcg aaacactgga gccgcatgga gtatgctaga
    35101 agggaagatg tggttcttta ctatagtgac actcattgca gtcgttgtga ttctcacatt
    35161 gatgattaaa aatcgtgaaa aaggtaatcg ctggttcatg attggattga gtttgattct
    35221 agctggtgcg attggcaatt ttatcgaccg tattcgtctt ggctatgtag tagatatgtt
    35281 ccagacagat tttatgagct ttccgatttt taacgtagca gatgtgacac ttgtatgtgg
    35341 agtgatttgt atgttaattt atattatttt agatgaaaag gatcaaagaa aaaaatgaca
    35401 gaacttaaaa cgacgattca ccatgaatct ggtaggatcg ataaagtatt gactaattta
    35461 ttcaccgatt acagtcgttc gcaaattcag ctgtggctga aaaatggagc ggtttctgtc
    35521 aatggacagc cagtcaaagc aaattacaaa gtaaaacaag gagacgagat tactgtcttg
    35581 atccccgaac cagaaacatt agatgtggtc ccagaaaata tcccgttgac gatcgtttac
    35641 gaagatgaag atgtagcggt gataaacaaa cctcaaggaa tggtcgttca tccttcagca
    35701 ggtcatacgc atggcacaat ggtcaatgcg ctgatgtacc atatcaagga tctttctacg
    35761 atcaacggcg ttattcgtcc aggtattgtt caccgaatcg acaaagatac ttccggactg
    35821 ctaatggttg ccaaaaatga ccaggcacac gaatcattag ctcagcaatt aaaagataag
    35881 acctctttgc gcaaatatgt tgctttagtc catggagaga ttcctcacga aaaaggaacc
    35941 atcaatgcgc caatcggccg ttcgaaggaa aaccggaaaa tgcaggcgat aagagaagat
    36001 gggaagtcgg ctgtcacaca ttttactgta ttggaacgtt tcgaaggatt tactctggtt
    36061 gaattaaaac tagaaaccgg aagaacacac cagatccgtg tgcatatgaa gtatatcggt
    36121 tatccgttag ctggagatcc agtatacgga ccgaagaaaa ctttgaaagg caatggtcag
    36181 tttctccatg cgcaaatgtt aggttttacg cacccgacta caaacgaaaa gatgatcttt
    36241 gaagcacctc ttccagaagt ttttgaaaaa actttagaaa aattaagaaa aaacattgca
    36301 ttttaattta gataacggta tagtaattaa caacgaaagc ataaaactta atagaattcc
    36361 tttaagttta gtcccgtgag gctaagaagg gtgggagcaa ggatacgtat ggtgcgccat
    36421 aagtagattg ctcattgaca tagtatagct atcagtgtat cagtatacgc tcagtctaaa
    36481 cattcctcct gtcctcttag ggcaggagtt tttttatgca aaaaagtagg gggaatgaaa
    36541 atgcaagcaa aagaagtagt agatcaggta accatgaagc gagcattgac tcgaattact
    36601 tatgagatta tcgagagaaa ccatagtatc caagatatcg tattggtagg tatcaaaaca
    36661 cgaggaatct acattgcatc aagaattgcc gaacgcctga agcagctaga agatatcgat
    36721 attcctgtag gagaactgga tatcacttta taccgagatg acaaaaaaga aaatccagaa
    36781 gaaccggaac ttcattcttc tgacatccca gtttccttag aaggaaaaga agttattttg
    36841 attgatgatg ttttgtatac aggacgaacg attcgagcag caatggacgc agtaatggat
    36901 tttggcagac caagaaaaat ttcattagca gtcctggtcg atcgtgggca tcgagaattg
    36961 ccaatccgtg cagattatgt tgggaagaac attccaacat ctcgggcgga agagatttta
    37021 gtagagatgc aagagcttga tggacaagat cgcatcatga ttttgaaaga ggaagattag
    37081 aggagagaga aacatgtcgg agaaaaaagg atttcataat gatgacgtgg tgttagatat
    37141 ccacgatcgc ccaaccccag gacattgggt aggattaagt ttacaacact tgttcacgat
    37201 gttcggagca accgtgttag tccccatctt agtaggtatc gatccaggta ttgcgctagt
    37261 cagttcagga ctaggtacat tggtgtactt atttactact aaaggaaaga tacctgcgta
    37321 cttaggaagc agcttcgctt tcattgcagc gatgcagctt ttgatgaaaa cagatggtta
    37381 tccagcgatt gcacaaggag cagtcacaac tggattagtc tatttgattg ttgcgctgat
    37441 catcaaaaag atcggttcag catggctgga taaaatcttg cctccaatcg tcgtcggtcc
    37501 agtcatcatg gttatcgggt tgggacttgc ggcaaatgca gcaacaaatg ctatgtataa
    37561 tcagggcacg tatgatttca agtatattgc tgtagcgctg atcacattag gattaacaat
    37621 tttcttcaac atgtggctaa gaggttttct cggattgatt ccgattttac taggtattat
    37681 tagcggatac atcgtagcct tgctgtttgg aatcgtggat actaagccaa tcgtagacgc
    37741 tgcctggttt gctgttccta actttgagat tccctttgta caatatgaac caaagctata
    37801 tcttggagca atcacaacaa tggcacccat cgcttttgtg acgatgacag agcatatcgg
    37861 ccatcttatg gtattgaata aactaactaa acgtaatttc tttgaagaac cgggtcttca
    37921 taagacgttg acaggtgatg gattagcaca aatcgtggca gcctttgtcg gaggtcctcc
    37981 tgtaacaagt tacggtgaaa acattggtgt tctggctatc acacgtgtcc atagcgtgtt
    38041 tgtgatcggt ggagctgcag tatttgcagt cattttagga ttcgtaggaa aattaagcgc
    38101 tttgatttta agtatccctg gaccagtcat ctcaggaatc agttttatct tatttggagt
    38161 aattgccgca agtggaatga agattttaat cgagaacaaa atcgattttg ataaaaagaa
    38221 aaatctgttg attgcttcag ttatcttagt agtaggaatt ggcggactcg tctttgaagt
    38281 aggcacattt acattgtcgg caatggcatt agcaactgta cttggcatca ttttaaatct
    38341 tatcttacca gaaacttcac gaagcgaaga aaactaagga ggaaacatta tgatcatcaa
    38401 atcagaacgt atcagcttaa agcatttatt gaccgttgag gcacttactg atcaagaagt
    38461 tatgggcttg atccgcagag ggcaggaatt caagcaaggt gctaattgga ctccgcaaaa
    38521 gcgccagtac tttgctacaa acctcttttt cgaaaatagt actcgcacac ataaaagctt
    38581 cgatgtagcg gagaaaaaac tagggctaga agtgattgaa tttgaagcaa gcacaagctc
    38641 tgtccaaaaa ggggaaacac tatacgatac agtactgacg atgtcagcat taggcgtaga
    38701 cgtagcggtt atccgtcatg gtgatgagaa ctattatgac gaactgatac aaagtaaaac
    38761 aatccagtgt tcgattatca atggaggaga cggaagcgga cagcatccaa cccaatgctt
    38821 gcttgatttg atgaccattt atgaagaatt tggtcatttt gaaggtctga aagttgccat
    38881 cgtaggtgat atcactcact ctcgcgtagc taaatccaat atgcagatgt taaaacgtct
    38941 aggcgcacaa gtattcttct caggtcctag agaatggtac gacgatgaat tcgaagtgta
    39001 cggacattat atgccattgg atgaactact agatcaagtt gatgtcatga tgatgcttcg
    39061 ggtgcaacac gaacgccatg atggaaaaga aagtttctca aaagaaggct accatcagga
    39121 acatgggtta acagttgagc gtgcgaagaa gttgcaaaaa catgcaatca ttatgcaccc
    39181 agcaccagtt aaccgtgatg tagaattagc tgattcatta gtggaaggat tacagtcgcg
    39241 gatcgttcag caaatgagca atggagtatt cgtcagaatg gcaatcttag aagcagtgct
    39301 aaatggaaaa gcgtaattat aggaggatga aaaaatgaaa acactcatta aaaacggtca
    39361 gatcaataca cgaaaaaata tgacgacacc agcagaaatt tggatagaag atgggcgaat
    39421 caaagcaatc ggtacaggat tctcagaagc cgaatttgat gaagtgtttg atgcaaaggg
    39481 acaattgatc acacctggtt tagtggacgt ccatgttcat ttgagagagc caggatttac
    39541 atacaaggag acgatcgaag ctgggactcg ctcagctgct cgtggcggtt tcactactgt
    39601 atgcgcgatg ccgaatttaa atcctgtacc tgatacggca gaaaagctga gacaagtata
    39661 tgacatcatt agaaaagatg cagttgtgaa ggtattgcag tatgcgccaa tcacagaaaa
    39721 tctgcgaagt gagaaattag ttgatcaaga agcactgatt gaagaaggtg cttttgcttt
    39781 cacaaatgac ggtgtaggtg tccaaacagc tgggacgatg tatctggcta tgaaagaagc
    39841 tgcaaaaaac aataaagccc tagtagccca tacagaagat gaatctttat tatttggcgg
    39901 cgttatgcac gctggcaaga aggcagaaga acttggtctt cccgggatct taagcgtaac
    39961 cgagtcttct caaattgcta gagatctgtt gctagctgaa gcaactggtg ttcattacca
    40021 tgtttgccat gtttctacta aagaaagtgt ccgtgtaatc agagacgcaa aaaaagcagg
    40081 aatccacgta acagcagaag tttcacccca tcatcttatt ttgatcgacg aagatattcc
    40141 cgaagatttc ggcttttgga aaatgaaccc gccgctaaga ggacgggagg acagagaagc
    40201 tttgatcgaa ggcttgcttg acggtacgat cgattgcatc gctacagacc atgcccccca
    40261 tggattagaa gaaaaaagcc aaagtttcat gaaatcacca tttggtatcg taggaagcga
    40321 aacagcgttt caactgatct atactcattt tgtagaaact ggtcgattca cgttggaaca
    40381 agtgatcaat tggttggcag ttaaaccggc tgagattttt ggtttgaatg caggaacgct
    40441 aacggttggt gcccccgctg atgtagcagt ttttgatatt acacaaactt gcacgatcga
    40501 taaagaagac tttttatcaa aaggggagaa tacacctttt atcggctgga aagtaaaagg
    40561 cgaaacacaa atgacttttg tcaacggaaa acttgtgtgg cagaagggag aataaaagta
    40621 tggaacgttt gcttatatta gaagatggaa cagttttcga aggaaaggct tttggtgcag
    40681 atgctagtgt cttcggagaa atcgttttta caacaagtat gactggttat caagaaacta
    40741 tcactgatca aagttttaac ggtcaaatca ttacatttac ctatcctatg gtgggtaatt
    40801 atggggtaaa tagagatgat tatgaatcga tttctccaac atgcaaagga gtagtagtaa
    40861 aagaacatgc acgtgtagct tcaaactggc gaaaccaaat gacgctagat tcatttttga
    40921 aacaaaaagg aattcctgga atatcaggta tcgatacgcg cgcccttact cgcaaaatcc
    40981 gtgaagtcgg aacgatgaaa gctagtatag ttgatgctgg agattccttc gaacatgcat
    41041 ttgatcagct gaaagcaacc gttctagcaa ccaatcaagt gcagcaagtc tcaactaaca
    41101 aaccttatcc aagtccagga acaggtcgga aagtcattgt cattgacttc ggcttgaaac
    41161 atagtatttt acgagaattg agtaaaagaa actgtaacct cacagtactt ccctataaca
    41221 cagatgccga gacgatttta gcgctatcac cagacggagt catgctgacg aatggacctg
    41281 gtgatccaaa agatgtgcca gaagcaatcg aaatgatcaa aaaaatccaa ggaaaagtac
    41341 cgatttttgg tatttgtctc ggtcaccagt tatttgcttt agccaacggt gcagatacct
    41401 ataaaatgaa attcggacat cgaggattaa atcacccggt acgagaagta gcgaccggaa
    41461 gaattgattt tacttctcaa aatcatggct atgcagtcga tgaagccacg attgatccag
    41521 aaaaactcat cgtaactcat gtagaagtaa atgacggaac agtagaagga gtcaaacaca
    41581 gagattatcc cgctttcagt gtgcagtttc atccagatgc tgcccctggt ccgcatgatg
    41641 cactacattt atttgatgaa tttatggaaa tgatggacgc aggaaaggag cagtaaacag
    41701 atgccgaaac gtacagatat caagaaaatt atggtgattg gatctggtcc gatcatcatt
    41761 ggtcaagccg cagaatttga ctacgcagga acacaagcct gccttgcctt aaaagaagaa
    41821 ggatatgaag tggttttagt aaattctaat cctgcgacga tcatgacgga taaagaagtg
    41881 gcagatcaag tatatatcga accgatcaca cttgaatttg tatcaaggat tcttcgaaaa
    41941 gagcgtccag atgctctcct tccaacttta ggcggacaga caggcctcaa tatggcaatg
    42001 gagctggcag catcaggtat tttagaagaa ttgaacatag aacttctagg aacaaagctt
    42061 tctgcgatcg atcaagcaga agatcgtgat ctattcaaac agttgatgaa agaattgaat
    42121 cagccaatcc ccgagtctga aatcgtcaat acagtagaag aagctgtaga ttttgcaaat
    42181 aaaatcggct atcctgtcat cgttcgtcca gcctttactt taggcggaac tggtggaggt
    42241 atgtgtgaaa atgaagaaga attacgtatc gtatcagaaa atggattaag tctttccccc
    42301 gctacccaat gtttgattga acgaagtatt gcaggtttta aagaaattga atatgaagtg
    42361 atgagagatt cggctgataa tgcaatcgtt gtctgcaata tggaaaattt tgacccggtg
    42421 ggtatccata caggtgattc tatcgtattt gcccctagtc aaacattgtc tgaccatgaa
    42481 tatcaaatgc ttcgtgatgc ttctctttcg attattcgag cacttaagat cgagggaggc
    42541 tgtaatgtcc aactcgctct tgatccacat agttttaatt attatgtgat tgaagtcaat
    42601 ccgcgtgttt ctcgttcatc tgcgttagca agtaaggcga ctggctatcc gatcgctaag
    42661 ctagctgcta aaatagcgct tggattaaca ttagacgaaa tgaaaaatcc tgttacagga
    42721 acgacttttg cagaatttga acctgcatta gattatgttg tagctaaaat tcctcgctgg
    42781 ccgtttgata agtttgaaaa cggagaacgg cttttaggca cgcagatgaa agcaactggt
    42841 gaagtgatgg ctatcggtcg gaatatcgaa gagtctttat tgaaagctgt gcgttcttta
    42901 gaaatcggta cacaccatat cgaacttcct gagttggcag acatcacgga cgatgaccta
    42961 atccaaaaag tggttcgagc acaagatgat cgcctgtttt atgtagctga agctattaga
    43021 agagggtaca ccatagaaga attagcggat ttgacaaaaa tcgatttatt ctttttagat
    43081 aaactgttgc atatcgtaga aatcgaacag cagttgaaac agcagccgtt ggatgacaag
    43141 atattgaaaa cagcgaaaca aaacggtttt acagacaaaa aagtggcgga gctgtgggaa
    43201 ataaacgaag aagccgttcg ccagatgaga aaagaaaatg atttgattcc tgtatataag
    43261 atggttgaca cttgtgcagc ggaattcgaa tcaaaaacac cttattttta tagttcctat
    43321 gaatatgaaa atgaaagttc acgatcagaa aagccctcaa ttctagtact gggatctggt
    43381 cctatccgta tcggacaagg ggttgaattt gactatgcga ctgtgcattc tgtcaaagca
    43441 atccagcagg caggatacga agcaatcatc atgaacagca atcctgaaac agtttcgact
    43501 gacttttcta tttcggataa attatatttt gagccattga ccttcgaaga tgtgatgaat
    43561 gtgatcgagc tagaacagcc gataggagta atcgttcaat ttggtggtca gacagcaatc
    43621 aacttagccg aacctttgac tgctgctggt gtaaaaatct tagggacaac gatcgaagac
    43681 ttggatcgtg cggaaaatcg tgatttattc gagcaagcgc tgaaagagtt ggatattcct
    43741 caacctttag gggatactgc tacaagtaaa gaagaagccg tggttatcgc ttcgagaatt
    43801 ggttatccag ttcttgttcg tccaagctat gtattaggcg gacgtgcgat ggaaatcgtt
    43861 gaaaaccagc gagatttaga agattacatg gaacatgctg tgaaagcatc tccagaacat
    43921 ccagtactag tggaccgtta cttgcttggt ggtgaatgcg aagtggatgc gatttgtgac
    43981 ggtgaaacag tactcatccc agggatcatg gagcatattg agcgtgctgg tgtccattca
    44041 ggggattcaa tggctgtgta tccacctcaa acattatctg aatcgatcaa gaaaacgatc
    44101 gaagattaca cgatccgctt agcattaggt ttgaattgtg tcgggatgat gaatatccag
    44161 tttgtcatcc atgaaaatga ggtttatgtg atcgaagtca atcctcgagc aagtcggact
    44221 gttccattct taagtaaggt gacaggaatt ccaatggcac aaattgcgac gaaagctatt
    44281 ttgggagaga aactaaagac acttggttat gaaaacggac tttatccaga aacaaaagaa
    44341 gttcatgtta aagcacctgt cttttctttt acgaaattgc agaaagttga tacttactta
    44401 gggcctgaaa tgaaatcgac tggagaagta atgggatctg acagacaatt agacaaagct
    44461 ttgtacaagg cattcgaagc ttctggatta cacttgcctg aatatggagc cgtattattt
    44521 acgattgcgg acgaaacaaa agaagaagca ctagaattag ctaaacgttt tacagaaatt
    44581 ggctatagtc tgataggaac gaaacataca gctgcttatt tcgaaaaaaa tggtctggtc
    44641 gttgaaacag tagcgaaaat ttctgaggaa gcagcagaaa aaaatgtagt cgacttaata
    44701 agagatggta aaacacaagt ggttgtcaat acgattgata aagatcgggg aaatgcttct
    44761 aaagacggtt tcatcatccg aagagaagca gttgaacatg gtattccatt gtttacttct
    44821 ttagacacag cagatgcgat cgttcgagtg atggaatccc gtgctttttc aactaaccct
    44881 atttaacggc gagcacatgt taagacaaaa agacgaaaaa ccgttgcagg aggggacaga
    44941 tgaaacaaga aatgatggaa atcgttcagc aaaaacagct ggcaccaaag atttttgaaa
    45001 tgacattgaa aggtgatcta gtaaaagaaa tgcagcaacc aggacagttc ttgcatattc
    45061 tcgtgccgcg tgcagacttg ctattacgcc gaccgattag cttgaatcat atcgaccacg
    45121 aaacaaatac ttgccgtatc atttatcggg tggaaggtga ggggactaga gtcttctcta
    45181 ctctttctgc cggagaccat ttagatgtca tgggtccttt aggcaatggt tttgatttgt
    45241 ccaatcttca tacaggagac gaagtattta taatcggtgg aggaatcggt gtccctcctc
    45301 tatatgagtt atcaaaacag ttagtcgaaa aaggaattaa gccaacgcat tttttaggtt
    45361 ttgccacaca tgaagtcgtt tattatgaaa aagaatttaa tcgtctagga gaaacaagaa
    45421 tcgctactga cgatggaaca tacggtatgc acggaaatgt cggtaattta ttgttaggaa
    45481 cgaagacgga acctacagct gtttttgcat gtggttcaaa cgggttattg aagacagtgg
    45541 aacagctgtt ttcttcgcat caaaatgtcc agttatcatt agaatcccgc atggcatgtg
    45601 ggataggggc ttgctatgct tgcgtttgtc atacgccgga agatgaaaca ggaacaaaaa
    45661 gtgtgaaagt ttgtgacgaa ggaccgattt ttaaaatagg agaggtaatt atatgaatga
    45721 gaatccttta gcggtcaaac ttcctggcct agaattaaaa aatcctatca tgccggcaag
    45781 cggctgtttc ggttttggca aagaatacgg aaaatactat gatttgaatc agcttggatc
    45841 gatcatggtc aaggcaacga ctccaaatgc ccgctttggc aatcccacgc caagagtggc
    45901 ggaaacacca tctggcatgt tgaatgcaat tggtttgcaa aatcctggtc tagacgttgt
    45961 tatgcataaa cttcttccag aattagagga gtacaatgag ttacccatca tcgcaaatgt
    46021 tgctggtgct tgtgaagaag actatgtaga agtttgtagc aaaattggcg aagcaccaaa
    46081 tgtgaaagca atcgagctga acatttcttg cccaaatgtc aaacatggcg gaattgcttt
    46141 tggaacagat tctgatgttg cttttcaatt aacacaagca gtaaagaaag tttcttctgt
    46201 tcctgtctat gtcaaattgt ccccaaatgt aacagacatc gttccaatcg ctcaggcaat
    46261 cgaagctgga ggggcagatg ggtttacgat gatcaatact cttcttggta tgcggattga
    46321 ccttaagaca agaaaaccga ttttagccaa tcaaacaggt ggactctccg gaccagcaat
    46381 caagcctgtc gctattcgtt tgattcacca agtagcaagt atatccagcc ttccaatcat
    46441 tgggatgggc ggcgtgcaga cagtggatga tgttcttgag atgtttatgg ctggagcaag
    46501 tgctgtagct atagggaccg cgaatttcac cgatccgtat atctgtccta aactaattaa
    46561 agagctccct gtgcgaatga gcgaactagg catcgaatcg ctggaaagat taatcaaaga
    46621 agtgagagag gaacgtgaac gatgaatcaa ctagaccaac gaccaattat tgcattagat
    46681 ttttcaacta ggcaagaggt agaagacttt cttcgatttt tcccgaaaga agaaaaattg
    46741 tttgtgaaaa tcggaatgga attgttctac caagaaggac ctgaaatcgt acgttatcta
    46801 aaagaggctg gtcataatgt atttttagat ctgaaacttc acgatattcc gaatacggtc
    46861 gaaaaagcaa tgcggggatt ggcaaagctt ggtgttgatc tcacctgtgt tcatgccgct
    46921 ggcggtattc gtatgatgga ggcggcaatg cgaggattag aagaggggac tccagaaggt
    46981 ggaaaacgtc cgctacttct agctatcact cagcttactt ctacaagtga agaagaaatg
    47041 catgccgatc aactaatcga agtttcttta gaaaaaagtg tgatccatta tgctagctgt
    47101 gccaaaaaag caggattaga tggtgtggtt tcttctgctt gggaagtaga agcgatcaaa
    47161 gaaatggcag gagacgaatt tgtttgctta actccgggta taagaccaga aggaacagtc
    47221 gcaggagatc agacaagagt agtcacacct tctcaggcaa aaaagatcgg ctcaactttt
    47281 atcgttatag gtcgaccaat cactcagtca actaatcctt ataaagcata tcaaacgatt
    47341 caaacagaat ggagctaacc taaaatgaat gttgaacaaa gtatcgcaaa agacttatta
    47401 gaaattgaag cagtattttt aaatccaagt aatcctttta catgggcaag tggaatcaaa
    47461 agcccaattt actgtgataa ccgaatcacg atgagctatc caaaagtccg aaaagagatc
    47521 gcaaaaggat tagcaagcaa aatcaaagaa gcttaccctg aagtacaagt gattgctgga
    47581 actgcaacag caggaatccc tcatgccgca tgggtagcgg aaatactgga tttgccaatg
    47641 gtctatattc gaagtaaggc gaaagatcat gggaaaggta accaaatcga aggacgtata
    47701 tttgaaggtc agaagatggt tgtgatcgag gatttgattt ctacaggcgg aagtgtcttg
    47761 gaagcagctg aagcagcaaa aagagaaggt gcggatatcc ttggtgttgc ggctatcttt
    47821 acgtatgagt tgccaaaagg aaaagcaaat ttcgaaaaag ccgaaatccc tcttatgacg
    47881 ttgaccaatt attctgtatt gattgaagca gctttagaag atcgttatat cgatgagcaa
    47941 gaattaactc tgctaaaaga atggaaaaaa gatcctgaaa attggcagat ttaagcgcgc
    48001 ttgatgaaga tagagaaatc cgatttgtgt ctccagaatc atcaattgga atagcaaaaa
    48061 tccaaaaact gattgcgaga agcatctttt catcaggtat gaaaagaaac ttctatcgtg
    48121 atcagttttt gattttttta aaattcattt ttctaagact ggttagcaga aagaagagaa
    48181 taatctcttc tcttttttct attatatata gattttctct attttttggt aagggagctg
    48241 tttcaaaaat ggtaaagagc ggaaatgttg cgaatatcag ttatttaatg atatgatatt
    48301 cggaaaaaga aatctcaaag ggaggtaact tttatggggt tgaatgaatt tattttagga
    48361 ttgaacaatt tagagacgat cacaagggca cctggatttt ttaaatatac agagcataca
    48421 gttgcagcac attcgtatcg tgtagcatct attgcgcaag ttctaggaga cattgaagaa
    48481 gcgaatggac aaccgatcga ttggaaactg ctctatgaaa aatcattgaa tcatgactac
    48541 acagagcgtt ttattggtga tatcaaaacg ccggtgaaat acgcaagcaa agagttaaga
    48601 gggatgctgc aaacagttga agaaaagatg acggacgaat tcatcaacca agagattcca
    48661 gctgagttcc aagaagttta tcgccgtcgg ttgttcgaag gaaaagatga aaccgtagaa
    48721 ggagaactat tagccatttc agataaggtt gaccttcttt atgaatcttt tgatgaaatc
    48781 atcaaaaata acccggaaaa agtttataag gagatgttca ttgaggcagt gactacgatt
    48841 aaaagctttg aacatcggcc ttctgttcag tatttcttta aagagatatt tccggaacta
    48901 ttgaaccagg aattttatgg aaaagaggcg tttttagaaa ccgtctcaac gattgtaaag
    48961 aaagaagaaa aataaaaatg atatgagatg cagggggaag tatagttgaa aaagaatatg
    49021 gatgtcgaat ggggctattc tggagacaaa ggtccggaaa attgggcgag tttatgtgat
    49081 tggtttgcga gaggtgcgga atttccattg cagtcgccta ttcatttgac caagggggaa
    49141 gaaaccaaaa tagaaggaga cacgctttcc tttcattatc aaaagcaacg gtttactgac
    49201 aaggaattca aaaacacgat tcatttagta ccttttgacc aactcagtta tgtagagttc
    49261 aagaaagaac gctattattt gacagatatt catttccact tgccttctga acatatcatc
    49321 aacggaaaac aggaagatat tgaatttcat tttgttcata tgaacagcaa aaaagagaat
    49381 ctagttgtgg gtgtcatgta ccagctaatg gtacaggaag gttggctgta taatcaagaa
    49441 aatggggaaa gttggaactt agaaaaacat gagcattggg taaatccgga tgtgttcttt
    49501 ccggagcaaa aaagccattt ccattatatt ggctcattga caactccgcc taccagcggg
    49561 ccaatccaat ggtttgtaat ggatcatgta ggaaaactga accaagagtt tcttctcccc
    49621 ttcgatggtc agtatgctcg accgaacaac cgaccaatcc agcccttgaa tggaagagaa
    49681 atttattatt tcgaggagtt tgagcaatga atattcagca gatgaaatac gtggtcgctg
    49741 tagcaaacaa tggtagtttc agagaagcgg caaagaaaat gtttgtctcc cagccaagtc
    49801 tttcagctgg tatcaaagaa ttagaaaccg agttagggat cacgctgttt actcgaacga
    49861 atcgaggggc atatttaaca gaagaagggc aagacttctt aaatcgtgcc gagaaaatat
    49921 tggtccagct tgatacatta gaaactcact atctaacgag tgaaaagaga gacaattttt
    49981 cgatcgctgc ccagcattat gatttcttag gtcctttgac cgcacagcta ctcgaggaat
    50041 tcaaagacga gatcaaacaa tttcggatca tggagacgac aacagcgaga gtgatcgaag
    50101 aagtcaaaga gcagcacagt gagatcggca ttatttatat gaatgagcat aatcaagctg
    50161 gtatcagaag atatttacaa caaggagagc taagtggccg cagcattgga aaattcaaaa
    50221 cgcatatttt tttaaggcaa ggacatccat tagctgaaca cgcagagatc accaaagaag
    50281 aattgttatc ttatcctcaa gttcgtttta cacaggatgg aaataatttc ccgtattttt
    50341 atgaagacct tatcgagatc cctgatcaag aaacagtgat ttatacgagt gatcgaggta
    50401 ctttgatgaa tatcgttctt gaaactgatg cttacgcttc aggatctggt attgtgattg
    50461 gagggattcg cgagcacttg cggttaatcc ctttggcaga aggcgaacta aatgaatttt
    50521 atatcattta ttcggcgaaa cgtccgctca gtgaaatcgc tcaaagattt atcaaagagc
    50581 ttactcggtt actggaccag cagagctaag aaaagcagat gagaacgctg tttattgaac
    50641 accgttgatc aaaaaagagg ggttgtgaca gaattttgtc acaactcctc tttttgtagc
    50701 aaac
//
LOCUS       DACDIE010000012        49759 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.012, whole genome shotgun sequence.
ACCESSION   DACDIE010000012 DACDIE010000000
VERSION     DACDIE010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49759
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.012"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            317..3151
                     /locus_tag="IV250_000804"
     CDS             317..3151
                     /locus_tag="IV250_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8768106.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNV
                     QEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDL
                     DMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNK
                     RQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVI
                     LDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTT
                     GIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLED
                     QIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
     gene            3342..3833
                     /locus_tag="IV250_000805"
     CDS             3342..3833
                     /locus_tag="IV250_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAP8768107.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            3936..4925
                     /gene="manX"
                     /locus_tag="IV250_000806"
     CDS             3936..4925
                     /gene="manX"
                     /locus_tag="IV250_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAP8768108.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            4958..5761
                     /locus_tag="IV250_000807"
     CDS             4958..5761
                     /locus_tag="IV250_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAP8768109.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            5782..6696
                     /locus_tag="IV250_000808"
     CDS             5782..6696
                     /locus_tag="IV250_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAP8768110.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            6779..7153
                     /locus_tag="IV250_000809"
     CDS             6779..7153
                     /locus_tag="IV250_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HAP8768111.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            7292..7537
                     /locus_tag="IV250_000810"
     CDS             7292..7537
                     /locus_tag="IV250_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP8768112.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            7709..8773
                     /locus_tag="IV250_000811"
     CDS             7709..8773
                     /locus_tag="IV250_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP8768113.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            9019..9444
                     /locus_tag="IV250_000812"
     CDS             9019..9444
                     /locus_tag="IV250_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP8768114.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            9437..9886
                     /gene="nusB"
                     /locus_tag="IV250_000813"
     CDS             9437..9886
                     /gene="nusB"
                     /locus_tag="IV250_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAP8768115.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            9983..10837
                     /locus_tag="IV250_000814"
     CDS             9983..10837
                     /locus_tag="IV250_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HAP8768116.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            10838..12175
                     /locus_tag="IV250_000815"
     CDS             10838..12175
                     /locus_tag="IV250_000815"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAP8768117.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            12180..12404
                     /locus_tag="IV250_000816"
     CDS             12180..12404
                     /locus_tag="IV250_000816"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAP8768118.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            12406..13296
                     /locus_tag="IV250_000817"
     CDS             12406..13296
                     /locus_tag="IV250_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP8768119.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            13305..14123
                     /locus_tag="IV250_000818"
     CDS             13305..14123
                     /locus_tag="IV250_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAP8768120.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            14230..14679
                     /locus_tag="IV250_000819"
     CDS             14230..14679
                     /locus_tag="IV250_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP8768121.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            14697..16379
                     /gene="recN"
                     /locus_tag="IV250_000820"
     CDS             14697..16379
                     /gene="recN"
                     /locus_tag="IV250_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAP8768122.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(16434..16550)
                     /locus_tag="IV250_000821"
     CDS             complement(16434..16550)
                     /locus_tag="IV250_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HAP8768123.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(16623..17579)
                     /locus_tag="IV250_000822"
     CDS             complement(16623..17579)
                     /locus_tag="IV250_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP8768124.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            17750..18118
                     /locus_tag="IV250_000823"
     CDS             17750..18118
                     /locus_tag="IV250_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HAP8768125.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            18288..18719
                     /gene="mraZ"
                     /locus_tag="IV250_000824"
     CDS             18288..18719
                     /gene="mraZ"
                     /locus_tag="IV250_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAP8768126.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            18740..19699
                     /gene="rsmH"
                     /locus_tag="IV250_000825"
     CDS             18740..19699
                     /gene="rsmH"
                     /locus_tag="IV250_000825"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAP8768127.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            19704..20105
                     /gene="ftsL"
                     /locus_tag="IV250_000826"
     CDS             19704..20105
                     /gene="ftsL"
                     /locus_tag="IV250_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAP8768128.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            20106..22292
                     /locus_tag="IV250_000827"
     CDS             20106..22292
                     /locus_tag="IV250_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP8768129.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPSED
                     PDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYRNL
                     EADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVTGW
                     SKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            22318..23280
                     /locus_tag="IV250_000828"
     CDS             22318..23280
                     /locus_tag="IV250_000828"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAP8768130.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            23294..24655
                     /locus_tag="IV250_000829"
     CDS             23294..24655
                     /locus_tag="IV250_000829"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAP8768131.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVSECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            24681..25769
                     /gene="murG"
                     /locus_tag="IV250_000830"
     CDS             24681..25769
                     /gene="murG"
                     /locus_tag="IV250_000830"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAP8768132.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            25784..27004
                     /locus_tag="IV250_000831"
     CDS             25784..27004
                     /locus_tag="IV250_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAP8768133.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            27160..28485
                     /gene="ftsA"
                     /locus_tag="IV250_000832"
     CDS             27160..28485
                     /gene="ftsA"
                     /locus_tag="IV250_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAP8768134.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            28508..29749
                     /gene="ftsZ"
                     /locus_tag="IV250_000833"
     CDS             28508..29749
                     /gene="ftsZ"
                     /locus_tag="IV250_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAP8768135.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            29766..30443
                     /locus_tag="IV250_000834"
     CDS             29766..30443
                     /locus_tag="IV250_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAP8768136.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            30457..31056
                     /locus_tag="IV250_000835"
     CDS             30457..31056
                     /locus_tag="IV250_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAP8768137.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            31071..31358
                     /locus_tag="IV250_000836"
     CDS             31071..31358
                     /locus_tag="IV250_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAP8768138.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            31407..32189
                     /locus_tag="IV250_000837"
     CDS             31407..32189
                     /locus_tag="IV250_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAP8768139.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            32210..32917
                     /locus_tag="IV250_000838"
     CDS             32210..32917
                     /locus_tag="IV250_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAP8768140.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            33179..35977
                     /gene="ileS"
                     /locus_tag="IV250_000839"
     CDS             33179..35977
                     /gene="ileS"
                     /locus_tag="IV250_000839"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAP8768141.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKSYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(36026..37552)
                     /locus_tag="IV250_000840"
     CDS             complement(36026..37552)
                     /locus_tag="IV250_000840"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAP8768142.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(37567..38214)
                     /locus_tag="IV250_000841"
     CDS             complement(37567..38214)
                     /locus_tag="IV250_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HAP8768143.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(38398..38727)
                     /locus_tag="IV250_000842"
     CDS             complement(38398..38727)
                     /locus_tag="IV250_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HAP8768144.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            38904..39632
                     /gene="sfsA"
                     /locus_tag="IV250_000843"
     CDS             38904..39632
                     /gene="sfsA"
                     /locus_tag="IV250_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAP8768145.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            39648..40661
                     /locus_tag="IV250_000844"
     CDS             39648..40661
                     /locus_tag="IV250_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8768146.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            40661..41938
                     /locus_tag="IV250_000845"
     CDS             40661..41938
                     /locus_tag="IV250_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP8768147.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            42001..44703
                     /locus_tag="IV250_000846"
     CDS             42001..44703
                     /locus_tag="IV250_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8768148.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            44855..45172
                     /locus_tag="IV250_000847"
     CDS             44855..45172
                     /locus_tag="IV250_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8768149.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            45202..45522
                     /locus_tag="IV250_000848"
     CDS             45202..45522
                     /locus_tag="IV250_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP8768150.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            45630..47090
                     /locus_tag="IV250_000849"
     CDS             45630..47090
                     /locus_tag="IV250_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8768151.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            47158..47379
                     /locus_tag="IV250_000850"
     CDS             47158..47379
                     /locus_tag="IV250_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8768152.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            47410..47592
                     /locus_tag="IV250_000851"
     CDS             47410..47592
                     /locus_tag="IV250_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HAP8768153.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            47592..48005
                     /locus_tag="IV250_000852"
     CDS             47592..48005
                     /locus_tag="IV250_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAP8768154.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            48128..49309
                     /locus_tag="IV250_000853"
     CDS             48128..49309
                     /locus_tag="IV250_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8768155.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
BASE COUNT        16560 a         8421 c        10747 g        14031 t
ORIGIN      
        1 tttactttgt ctttttacac ttaatatttt tttgccaatt tggaatcagg atgttttgat
       61 aactatcgtt ttcaattgat ggaagctata ttgtttgcta tcagtgaaat aattatatgg
      121 taaacagtgt tctttttctt gtttccatta taaaaatcgt atcgggattt ttcgttcttt
      181 taaattgaac atttctatct atccgcgtgt tatcaacaaa acaaaatcag gtaaatagtt
      241 gacgaaacag aaataaatac atagtattat gttttgaagc ctaagtacaa tagtctatag
      301 aaaaggagcg ataccattgg caaaacgcat tgaccgaatt tatatgtatg tcaaagaaaa
      361 aaccgctcat ttaacaaagg cagaatatga tcaaggagta acaacacagg aaattgctga
      421 agtgttaggg attcagcgaa caaatagcag taaagatctg aatcagctag tgcgcgaagg
      481 aaaactgata aagacagacg gacgaccagt acgttacata tatcaaacac tcgttacgca
      541 cgtaaagcca gttacaaaac atgtagtcag ttacaaagaa acgccggtct ctgaagaaaa
      601 aaatacactc caaatagata ctaaggatat ctttgcaaaa atcattggag ctaacggcag
      661 catgaaaaat tcagtggaac aggcaaaagc tgcgattttg tatccgccaa gaggattaaa
      721 ttgtttgatt acaggaccta ccggatcagg aaagacttat tttgcacacg cgatgtatca
      781 ttttgcaaaa gcgcatcatg taatagatga ggaaacggaa ctgattgttt tcaattgtgc
      841 tgattatgcc aataaccctg agcttttgat gagtcattta tttgggtacg caaaaggtgc
      901 ttttacaggc gcagatgaag aaaaaatggg gattatcgat caagcggatg gcggtatgct
      961 gtttttggat gagattcacc ggttgccgcc tgaaggtcaa gaaatgatct tttactttat
     1021 ggatcatgga acttacagtc gtcttgggga aacaacgaaa tcccatgaag caaatgtacg
     1081 gatcgtaggt gcgaccacag aagacccagg ttcttctcta ttagaaactt ttgttcggcg
     1141 tatcccaatc aatatcaaac ttccggcttt tgaaaaacgt cctgcaaatg agaaagtcga
     1201 tttagtaaaa atcatgattg cccacgaagc aaaccgcacg cagcgaaaga tttctttgac
     1261 agaagatgta gcaaaagcat tgatcggcag tgtgacctat ggaaatatag gacaattgaa
     1321 gtcaaatatc cagttagtat gtgcacgagg atttctaaat catatgcaat cacccgaaat
     1381 ctcgatcaca atcgatgatt tgacagaagg gattcgtagc ggattgattc aattagcaag
     1441 caaccgcagt gcaatggcag agctttctaa aatattggag ccaaagataa ctgtagcacc
     1501 aaatgatacg atcatgaaaa tccaatcgga ctcctatgag ttaccttata atctatatga
     1561 tatcatcgga gataaagcag ctttactgaa gtcggatggt ttggatcaag aagcaatcaa
     1621 tcatttcatt tctacagata tcaacatcca tctgaagtct ttctataaag accatggttt
     1681 ttcttttaat gcagacagca aattagcaga atttgtagat ccgaaaatca tagaagtgac
     1741 caatcagatt tatgtaatgg tcaaaagatc tttaccatat gagtttcagc aaaactttat
     1801 ttatgctatg agcttgcata tcagttcttt tttgaaaaaa atcaacttag gagaagaacg
     1861 gcatacgaat gataacatcc gtgagatggc ttttgatttt ccagatgaat atgctgttgc
     1921 gaaagaagtg cgtagttata tcgaaacgta ttttcaagtg gagattccag aaagtgaaga
     1981 ctattacttg accgttctac tcgtctcgtt acgggcaaat caagcaagcg gcagaatcgg
     2041 tgtagtcgtt gcagctcacg gaaacagcac agcaagcagt atggtccaag ttgtccaaca
     2101 gttgttagat gcagagaacg tacgagcagt agacatgcct ttggatatgg atccgaaaac
     2161 agcattggac cgaattgaac gcaatgtaca agaagtagat gaaggaagcg gggtcatact
     2221 cttagtagat atggggtccc ttgcgtcgtt taattcgcaa attcaaagag acactggaat
     2281 tccagtacgt actgttgata tggtaacaac ttctctagtt ttggaaactg tacgaaaggt
     2341 ctcagtatta gggacagatc tagatatgtt gcatgaatca ttgaaaaatt ttcgaggtta
     2401 cgcagaaata tcttctgtag aatctgttca ggaagcaaaa cctattcata agaaaaaagc
     2461 tgttttggct atctgcgcat ctggcgaagg tacagcacaa agaatcaaag aattgatcga
     2521 acgagcggta aacaagcgtc aagaaacaga attagaagtt ctagctttgt cgattgtgga
     2581 agtgaaagaa gaactgccag ttatccaaaa agaatatcag atcatagcta ctactgggat
     2641 tactgatttg aatatccaag ccccatttat tcctttagaa cgttttattg atcaaaatat
     2701 cgaagtcatt ttggatcagt tgctgatgga atcagaattg gaggaaactg aattcatctc
     2761 cctagatgaa gagtcagcta aaaatacgtg tgttgagttt atctctgata acttcatctt
     2821 tattaatggt agtaagttaa tcgatccaat gtggcaattt tctacacaaa tctctcagac
     2881 gacgggaatc ggcgatgaag aatatggatt caaaatcaac ctagtgatgc acacagcagg
     2941 aatgatcgaa cgtattattc ggaatgaacc attgactgtg gaagaaaacg aattgacaaa
     3001 tacaacgaat gatcctcttt atagccaatt ggcagcctca gttgttctgt tggaagatca
     3061 gataaaagta aaagtgccga tagaagaaat gtattatctg ctacgtctag tccataatca
     3121 gttagataaa aaagaataca ctgtgccatg aatacacaaa aacgatacac ttttatagtg
     3181 tatcgttttt gtgtattggt taagaatcct ttaaattcaa ttagtgtatc gtaaaaaagt
     3241 tggcacgctt tttgcttata gtagtgtgtg ctaggaggtg gttgttcaca cagaaatgat
     3301 aggggtttct gtgaagtagt gaggaagaag gagttttgca gatggatatt cgattggcac
     3361 gtatagatga tcgtttaatc cacgggcaag tcgcaacagt ttggtcgaag caaacaggtg
     3421 ttgaacgtat cattgtgatc agcgatgtag tcgctcaaga taaattgaga aagtttctac
     3481 taaaagaagc tgcaccgccg gggatcacag caaatgtaat aaccgtgcag aaaatgattc
     3541 aaatctatca tagtggatta ttatcacgag caaaagtgat gcttttattc accaatcccc
     3601 aagatgttga agcggttgtc cgctgcggtg tacagttgaa gtcgttgaat atcggcggta
     3661 tgagttttac tgaaggcaaa tcaatgatta ccaattttgt ttcagtaaat caacaagata
     3721 ttgattcctt tcgttacttg aacagtcaag aagtggagtt agagattcga aaagtacctg
     3781 ctgacaggaa aatccttttg atggaactac tagcgaaaat caaaaaggtg tagcaagaat
     3841 ggttgttgga aaaggtcaaa taattctgtt gtaatggtta gcaattatta atatttttga
     3901 atttgttaca acaaaagtaa ataggaggta taaatatggt agggattatc ctagcaagtc
     3961 atggacaatt tgctgaagga atcttacaat ccggttcaat gatctttggc gaacaagaaa
     4021 acgtcaaagc cgtgattttg aaaccaagtg aaggtccaga tgacttgaga gccaaattgg
     4081 aagaagcggt tgcttctttt gataatcaag acgaagtgtt attcttagtt gatttatggg
     4141 gcggaacacc gttcaatcaa tcaaacacat tattcgaaga gcacaaagat aaatgggcaa
     4201 tcgtcagcgg tttgaattta ccaatgttga ttgaagccta cgcatcacgc ttttcaatgg
     4261 aatctgctca tgaaattgca gcacacatca ttgaaacagc aaaagacggt gtaaaagtaa
     4321 aaccggaaga attagaaccg gcagaagcac cgaaagcagc agttgaagac gcacaaccta
     4381 aaggagcact tccagaagga actgtggtcg gagacggaaa aatcaaatat gtattagcac
     4441 gtgttgactc acgtctgtta catggacaag ttgcgacagc ttggacaaaa gcagtgcaac
     4501 caaaccgtat tatcgttgtt tcagatgcag tatcaaagga tgatctgcgt aagagactaa
     4561 tcgaacaagc tgctcctcca ggtgtaaaag caaatgttat tcctattagc aaaatgatcg
     4621 aagtagcaaa agaccctcgt tttggaaata ccaaagcatt gcttttattt gaaaatcctg
     4681 aagacgtatt gacagctgtt gaaggtggcg tagacatcaa agaattaaat gttgggtcaa
     4741 tggcgcattc ggtcggtaaa gtcgttgtaa gtaaagtatt gtctatggga caagaagacg
     4801 ttgaagcgtt tgagaagctt gaacaaaaag gtgtgaaatt cgatgtacgt aaagtaccta
     4861 atgattcacg cgataacatg gatgatattt tgaaaaaagc aaaagctgaa ttagcaaaag
     4921 cgtaataaat aatattaaaa tttataggag gcttatcatg tctattattt caataatttt
     4981 agtcgtatta gttgcctttc tagctggtat ggaaggtatt cttgatgaat ttcaatttca
     5041 tcaaccacta gtggcatgta cattgatcgg tttagttacc ggtaacctcg aagctggtat
     5101 cgtacttggc ggtacgcttc agatgatcgc tttaggttgg gcaaatatcg gtgccgctgt
     5161 agcaccagat gctgcattag catcagtagc atcagcaatt attttagttt taggtggaca
     5221 aggtgttaaa ggtgttcctt ctgcgatcgc gattgccgtt cctttggctg ttgcagggtt
     5281 gttcttaaca atgatcgtac gtacaatcgc tgttccaatc gttcatttga tggatgcggc
     5341 tgctgaagaa ggaaacatcc gtaaggttga aatgtggcac atcattgctg tatgtttaca
     5401 aggggttcgt atcgcggtcc cagcagcagc gttgctattt atcccagcac aaactgtgca
     5461 atcattcctt gaatcaatgc cagcatggtt aacagatggt atggctatcg gtgggggaat
     5521 ggtcgtagct gtaggttacg ctttggtaat caacatgatg gctacaaaag aagtatggcc
     5581 attcttcgtt attggtttcg ttgtagctgc aatttctcaa ttaacattga ttgctctagg
     5641 tgctttaggt gttgcattag ctcttattta cttgaacctt tctaaaatgg gtggttcatc
     5701 aaataatgga ggcggaagca actctggtga ccctttaggc gacattttga atgactatta
     5761 atcttgaagg aggaaaataa aatggcagaa gaaaaaatca aattatcaaa aaaagatcgt
     5821 ttagctgttg cgtggcgctc taccttcatt caaggttcat ggaactacga acgtatgcaa
     5881 aacggtggct gggcattttc tatgattcca gcaatcaaaa aattatacaa aacaaaagaa
     5941 gaacgttctg cagcattaaa acgtcacttg gaattcttta atacacaccc atacattgct
     6001 tctccaattt taggggttac attggctctg gaagaagaac gtgcgaatgg cgcgcctgtt
     6061 gatgatgtag cgattcaagg ggttaaagta ggtatgatgg gtcctttagc tggtgttggt
     6121 gacccagtgt tctggtttac agtacgtcca atgctaggtg cactaggtgc ttcattagct
     6181 atgggaggaa atatccttgg accaatcatc ttcttcttag gttggaacat tattcgttgg
     6241 gcattcatgt ggtatacaca agaatttggc tacaaagctg gttctaaaat tactgatgac
     6301 ctttctggcg gattgttaca agacgtaaca aaaggtgctt caattttagg gatgttcgta
     6361 ttagctgccc ttgtacaaag atgggtatca atcaaattct taccagttgt atcaactgta
     6421 aaattagata aaggtgctta catcgaatgg gataaattgc cttcaggtgg tgaaggaatc
     6481 aagagtgcat ttgaacaagt taataatggc ttagcccttt ctcctgaaaa ggtaacaaca
     6541 ttacaagaca acttagacca attgattcct ggtttagctg cattaggatt aacattgtta
     6601 tgtatgtggt tgcttaagaa aaaggtttct ccaatcgtta tcatccttgg tttattcgta
     6661 gtcggtgttg ttggacacgt catcggactt ttataatcca taaaaatata gctcgggcgt
     6721 tgtcccgagc tttttttata tagtatactg ttcataaaca aatatctgga gggagaaaat
     6781 ggttcaatca atcaatacaa aagtagactt ggtaattgat gctacttcgt ttacgggtct
     6841 gacagattac gggcaaatca tgataggtga taatggtttt gagttttacc atgcgcgaga
     6901 tagacgcaaa tatatccaaa ttccatggga agaagtggat tatgtactag catcagtcat
     6961 gctaaaggga aaatggattc ctcgatatgc cattcaaacg aaaaaaaatg gtacatacac
     7021 attttcttcc aaagaaccta aaaaagtgct tcgtgcgatc agagaatatg tagatgaaga
     7081 tagaatggta caatcattga gtttctttga tgtagtgaaa cgtggtattc gtagtatgtt
     7141 taaacgcaag taaaaaataa agaaaacttc taagaggagg ttttctttat ttttttactt
     7201 tgcagaagaa aatatgaaat agcaaaacca attatttgtc ccctttaaat cccatatgct
     7261 aaaattgatt agcaaagaag gaggttatat tatgagttgg ttatggacat tgattgttgg
     7321 agccgtgatc ggagcaattg caggtgcgat caccagtaaa ggaaattcga tgggctgtgt
     7381 aacgaatatc attgctggac tagtcggttc aagtattgga caagcgattt tcggcgaatg
     7441 gggaccaaaa gctgcaggta tggcacttat tccatctatt ttaggcgcag tgattttggt
     7501 agcagttgtc tcatttttct ttggaaaacg atcataagtt tttacaaaat agtaaactaa
     7561 aaggagttgc agcgagactg tgactccttt ttggctttat ttatttaaaa taaaaagaag
     7621 ttttccggaa atttttattt aactgtcgtg tacgaagaaa agtttgctat aatggaaaaa
     7681 agcgtgcaaa gaaagaggga gtttcattat gatgctacgt gtagaaaaat tgcgcaaaaa
     7741 aatgcaagaa gaaaatttgg attcattttt ggttaccagt ccgtacaatc ttcgttactt
     7801 aacgaacttt acaggtacga caggattagc tgttattact ttggaaaaag cattttttat
     7861 tactgatttt cgttataccg agcaggcggc agcgcaggct caagggtttg aaattattaa
     7921 aaatgtcgga cctatttttg atgaggtagc aaacttagta cgcaaagaag aactgcactc
     7981 attagctttt gaggaaacaa cagtctcatt tttagaatat tcggttttag aagaaatcat
     8041 cgatgcggaa ttagtacctg tttcacagat gatcgaagaa ctcagagaag tgaaagatga
     8101 agaagaaatc gctattatcg aaaaagcatg tcatattgcg gatatggcat atgatcacat
     8161 cctgaaaatg atccaaccgg gcatgactga gattgaagtc gcaaatcaac tagactttta
     8221 tatgcgttct cttggcgctt caggtgtttc ttttgatacg atcgttgcta gcggattgcg
     8281 ttcggctatg ccgcatggtg tagcaagcaa aaaaatcatc gaacaaggtg acttgatcac
     8341 attggacttt ggttgctatt acgaagggta cgtttccgat atgacacgta cattcgctat
     8401 tggtgatcct ggggaaaaat taaaagaaat ttatcaaata gtcttagatg ctcaattagc
     8461 agttattgat gctgcaaaac caggaatgac tggtgtccaa ttagatgcag ttgctagaga
     8521 ttatatttca aaacatggtt acggggaagc atttggacat tctacaggac atgggatcgg
     8581 tttagagatc cacgaaggac caaacgtttc ttctcgtgca gaaaagcaat ttgtaccagg
     8641 aaatgtgatc actgatgaac cagggatcta cttaccagga atcggtggag tacgtattga
     8701 ggatgacttg ttgatcacag aagatggaaa tcgtgtgttg actcgttcac caaaagaatt
     8761 gatcatctta tagtttcatc aatagaagaa gtaagcgtaa aaatcagtgt tcacagactt
     8821 tgttatagaa aatcactcga tttaatgata cactatagaa gaacttttaa gctgtaagag
     8881 ataataaagt tgggtgtttt ctccaattca tagcagtttg cagctgatcg gcggcagttt
     8941 tcttaccacg tgatcactat gccgcatcat agagaattcg gatgtatctc gtgcttaggc
     9001 aaagggagga ataaataaat ggcagatgaa aaaaatttga ttttaaacgc aaaccaagat
     9061 ttaggagaga tcgtgatcgc tcctgaagtc atcgaagtaa ttatcgggat tgcagcttct
     9121 aaagtagaag gcgtatacgg aatgcgcgga acttttgcca gcaatgtcac tgaattatta
     9181 ggacgtgcag ctcacggaaa aggtgtttac ttagcaaacg aggaagatgg cttgaaagta
     9241 gatctttact gttatctaga atatggtgtt tcagttccta aagtagcaat ggaaatgcag
     9301 gaacgggtca gccagcaagt ccttttcatg acggatatcg atttatctga agtcaatatc
     9361 catgtagtag ctgttgttcc tgaaaaatta gaacaaccaa caatcgaaga actttttgaa
     9421 gacgaagagg aagagaatga ataaagaatt atcaagacac gagattcggg aaatggcatt
     9481 gcaggcttta tttccattag acttcaatgc agacctaaca aaagaagatg cgattttcaa
     9541 cgcaatcgaa ttagaccacc gcgacatgat caacgaagat gaatcagagt ttgttcctgt
     9601 ttatcttgat acgctagttg gcggtgtctg tgcaaaaaaa gatgaattgg ataaagtgat
     9661 tgaaaaacat ttaaaaaaca attggcacat caatcggatc tcaaaaatgg atttggtgat
     9721 cttgcgtatc gcgatttttg aaatgactta tgtgacagat gtaccagcgg ctgttgcttt
     9781 aaatgaagca atcgagttgg caaaaacatt tagtgatgac cgttcacgta aattcgtcaa
     9841 cggtgtttta tccaatgtat tgaaagaatt agaagctggg gcgtaagtcc tagctttttt
     9901 ctatgaatca tcataaacct gttataaatc gggaaaattc tatggtagaa tgaaaacaca
     9961 aaaaatatct gggagtggtt agatggcaga attgattaac ggaaaagaat tagctgaaaa
    10021 aatgcaggca gagattgctg aaaaaatcca aaaattgaaa gataatggta tccatcccgg
    10081 attagtcgtt ttattggtag gggaaaatcc agccagccaa atctatgtaa gaaataaaga
    10141 acgtgtagca aaagaaatcg gaatccattc attggtagag cgttatcctg agacgatttc
    10201 tgaagacgaa ctacttgcag aaattgaaaa atacaatcaa gatcctagtt ttcatggtat
    10261 attggtccaa ttaccattgc caaagcatat agatgaagaa aagatcctgt tagctatccg
    10321 ccctgaaaaa gacgtagacg gttttcatcc gatgaattta ggcagactcc ttgcgggtaa
    10381 accggacaag attccttgta ctccatacgg catcatgaaa atgtttgaag cttattctat
    10441 cgatccggcg ggtaaacgag cagtagttat cggacgaagc aatatcgtcg gtaaaccgat
    10501 ggctcagcta ctcttgatgg cagatgcaac tgttactgtt gctcattcta aaaccgaaaa
    10561 tctggctgaa ctggcacaag aagcagatat tttagtcgta gcaataggac gtggacattt
    10621 tgtcacaaaa gaattcgtaa aaccaggtgc tgtagtgata gatgtaggga tgaatcgtga
    10681 tgctaacggt aagctgatcg gtgatgtgaa atttgatgaa gtggagccta ttgcaggctg
    10741 gatcactcct gtgccaaagg gtgtagggcc aatgactatc acgatgctga tgtatcaaac
    10801 cgtaaaatct gccgaagctt ttggagcagg tgaatagatg gcacaagaat atttaactgt
    10861 cactacatta acgaagtatt tgaaaagaaa atttgatgcc gatccttatt tagaacgcgt
    10921 atatttaaca ggcgaaatat cgaatttccg tttacgtgcc aatgcccatc aatatttcag
    10981 cttaaaagat gaccatgcaa aaatatctgc tattatgttc aaaggagcat tccaaaaatt
    11041 acgattccag ccaaaagaag gaatgaaagt ccttgtcgtt gggcgtattt cattatatga
    11101 agcgggaggt tcttatcaaa tctatgtaga gcacatggag cctgatggaa taggtgcttt
    11161 atatcaagaa ttggaagaac gaaaagaaaa gctgatgaag gaaggccttt ttcaagggcc
    11221 taaaaaaatg ctgccacgtt tcccaaagcg aatcgcggtc cttaccagtc caagcggagc
    11281 ggttatccgc gatatcatca cgacggtgaa aagaagatat ccaattgtcc aattggtttt
    11341 gttcccaaca gttgtacaag gaaaccaagc agcggacaat gttgtcagga atatccagag
    11401 agtagaagag atgggcaatt tcgatacaat gatcatcggt cgtgggggtg gttcgattga
    11461 ggatttatgg ccgtttaatg aagagcgggt tgctcgggcg atttatcaag ctaatacgcc
    11521 ggtgatttca tctgtaggac atgaaacaga taccacgatc gctgatttag tagcagatgt
    11581 tcgggctgcc actccaacag cagctgcaga attagctgtt cctgttttgt cagaagagtt
    11641 gatgcgcatt caagaacggc aagctagatt gcagcaggcg tttgtacgcc agatccagcg
    11701 caaacaagaa cggttcgagc gtgctcgacg ttcctatgta tttcggcagc ctgaaagatt
    11761 gtatgaaggt cagaccatca aattagacca gttgaaacag cgattatatc aagctacaca
    11821 gcaaatttat catgcgaaag aaaaacaaac gatcgcatta agccaccaat tggaacaagt
    11881 ggctccgctt tatcgagtta aagcagctcg ccaagaaact gcttatctag aaaaacgctt
    11941 agaagagaaa atccaacaat atatgaaaca acagcagcag cgttttgctc aagctgttca
    12001 atctcttgat ttgttgagtc cattgaaaat catgggaaga ggctacagct atacgacgtt
    12061 aaatgatcaa gtagtaaaaa gtgtcaccga gattcatcaa ggggatttgc tgacgatcca
    12121 ttatcaagat ggaatcgtta aaggacaagt acaagagata gacaaaggag aataaaaata
    12181 tggaaaatcc aacttttgaa gagtcactgc aagaattaga aaaaattgtc atgcagttag
    12241 aacaaggaga cgttccatta gaatcagctc tagattcgtt taaacgagga atggaattaa
    12301 gcaaacattg ccaagataca ttagctaaag cagaagagac cttgacgaaa atgatgacag
    12361 aatcaaatga agaagttgat tttgacggaa acgaggaaag ctgagatgag attaactgac
    12421 ttttcagcta ttcatctacc tgctgtagaa aaagaaatct tgtctttttt agatgagcat
    12481 acaacggata gaggactcta tgatgcgatg agttattcag tgaaagcagg cgggaaaaga
    12541 atacgtccct tattactttt aacggccgtt gcttcctttg atgagcctat tgatgttccc
    12601 gtctatcaag tagcagctgc tttagaaatg gtgcatacgt attccttgat ccacgatgat
    12661 ttgcctgcga tggacaatga tgatcttcgt agaggaaaac ctaccaatca taaagtattc
    12721 ggcgaagcat tagctatatt ggccggagat ggtttgctta caggagcatt ccagctaatc
    12781 agtatggcac acttaggtaa ttctcctaag cttctgctgc ttcagcaatt agctgtttgt
    12841 gcgggatcac aaggtatggt tgctgggcaa gctgcagata tcgagggcga atcgaaaaag
    12901 ctttctttag aagaattagc atttattcat gaaagaaaga cagggcattt gattcgttat
    12961 gccttgctgg ctggtggcat attagcgaag caacctgaag aaatcttatt gctacttcaa
    13021 cgattagccg aacatttagg tttagctttt cagataagag atgatctact cgatgtgatt
    13081 ggtacaacga aaacattagg gaaaacagct ggtaaagatg aacgtatgga aaaaaatact
    13141 tacccgcgtt tattaggcct agaaaagaca cgtgaggcgt tggagattga gctgttatcc
    13201 gccaacaaga tcatagacaa attagaagag gacgtttttt cttttgatgg cgggctgatg
    13261 cgccagatga tcaagcaatt tgatgtggag aaatgaggga aagaatggaa aaagaacgcg
    13321 tagatgtatt agcggtcaaa caaggattat ttgaaacaag agaacaagcc aaacgttcag
    13381 tgatggctgg tttgatttac aacgaaaaaa acgagcggtt tgataaaccg ggagaaaaaa
    13441 ttccagtgag tagtgaatta agagtcaaag gtaaaaaact tccttatgtt tctcgagggg
    13501 gattaaaatt agaaaaagca ctcaaagcat ttgatctatc tgttgaagga aaaacattgt
    13561 tagatattgg tgcatccact ggcggattta ctgatgcagc tttgcaaaat ggcgcaaaaa
    13621 tgagttatgc tttagacgtt ggctacaatc agctagcttg gaaaataaga caagatccta
    13681 gagtcgtcgt aatggaaaga gtgaattttc gttatgcaaa acccgaagac tttaccttag
    13741 gaattccaga agttgctgtt atcgatgttt cttttatttc actaaaactg atgctgcctc
    13801 cattacatgc tattttaaaa gaagagggag aagtgattgc tttaatcaaa ccccaattcg
    13861 aagcaggcag agaagctgta gggaaaaacg gaattgttcg tgaccctcag acccatcaaa
    13921 aagttttaga agacattttg tcatttgcgg cacaaggtgg atatgatgtg ctggaattaa
    13981 gttattctcc gatcacgggg ggagaaggaa atatcgagtt cttggctcac ttgaggaaag
    14041 tacccgaaag cggaacaatc aattcagcta ttaatatggc agaagtggtt tcaaatgcac
    14101 acgaacagtt tgaccataaa taaattgtaa ctgtgaaaat caagtgagaa gtatgcggat
    14161 tcttctttat tataccaagg aaataagcta tcattgatta agaagaatat gctaaggagc
    14221 gaagacgtaa tgagaaaaaa agatcggcat cgtttgatta ctcgattgtt gagtgagcaa
    14281 gatatccgaa agcaggaaga atttgttgaa atcctgaaaa gcaaaggaat atctgttact
    14341 caagctacga tttccagaga tataaaggaa ctgaaactga tcaaggtacc ggcttcagat
    14401 ggcggctacc gttatagcat accagctgaa acaaacgaag atgtaaatac taaattggaa
    14461 aaactattaa aagacgcttt cgtggcagtt gaccaaatgg aaaaatttgt tattttgaaa
    14521 acattgccgg gaaatgcttc ggcagcagca aatttgattg ataaacgtta caagaaagaa
    14581 ttattttcga ttatcaatga tgacgataat gttttgatga ttacacgaac tgaagaccag
    14641 gcagttttat tgaagaaaga cttcctgcat tacctttaag ttgaggagcg aatgtgatgc
    14701 tacaagaaat cagtattact aactttgcga ttattccgga attgcggctt tcttttcacg
    14761 aaggaatgac cgcgctgacc ggagagactg gtgcgggtaa atccattatt atcgatgctt
    14821 taggtctgtt agcaggagga agaggttcaa gcgattatat acgtcaagga gcagaaaaat
    14881 gtgtgcttga agggttgttt gaactaccaa aacaagaagg attctctgaa ctgatggttg
    14941 aattagggat cgaaacagat gaggacaatc tgattgttcg acgggatatg tccttaactg
    15001 gaaaaaatgt ttgtcgagtg aatggtcata ttatcacatt agctaatctg agaagaatcg
    15061 gcagttattt agtagatatc caaggacaaa acgaacatca ggaactgctc cagcctgaat
    15121 cacatcttgc tttattggat cgttttggag atgtagcttt ccaacagaag aaaaagagtt
    15181 atcagcaaga atatatctct tatcgtgagc tggaaaaaag ggtacgaaaa gtgcagcaaa
    15241 atgaaaaatc ctatgttcag agaatagata tgctccattt ccagcaagaa gaaatagctg
    15301 ctgctgattt gcaagtcgga gaagaagaga aattaaaaga agaaagagaa aaacttagca
    15361 attatcaaaa aattgctgat ggattagctg caggttacgg agcactgacg aataatgaac
    15421 aaaacagtgt agatggtgtt ggcttggccg tttcagaact gcaagggatc gcccatctag
    15481 atgtggagta tgaagcaatt tatgaaaata tccaaagtgc ttactatttg ctgcaagatg
    15541 caatcggcga tatgagtcgt cagattgatt tattggagtt agatgagagt cgtttagaag
    15601 aagttacgca acgtttagaa ttaatccgac agttgaaacg taaatacggc gaatccattg
    15661 agtcaatatt ggcttactat gacgaaatca ccgaagagct agcttcttct gatttttcag
    15721 aaggtcaatt ggacaaaatg aaagaggaac tagagcaaaa agagcttcta cttcagcaac
    15781 aagccgcaga tcttcatgaa gcgcgtaaaa aaatcgcaaa agaattggaa aaatccattc
    15841 tgcatgaatt gaaaagtctg tatatggaaa acactgagtt tgaggtacgt ttcttaaaag
    15901 aagaaaatag acaattgaat catgatggat ttgaccaaat cgaattttac attacgacca
    15961 atccaggaga gccactgaag cctttggtga aagtcgcttc tggtggcgaa ctgtctcgga
    16021 tgttgcttgc tttgaaaact attttttctt ctgagcaagg tgtgacaagt attatttttg
    16081 atgaagtgga tacaggtgtg agcggaagag ttgcacaggc catcgcagat aaaatttctc
    16141 aaatatcgaa atactctcaa gttttgtgca tcactcatct gcctcaagtt gctgcagtcg
    16201 ccgactatca atattatatt gtgaaagcag tgattgatgg acgtacgcaa acttctgttt
    16261 cagagttgaa aacaaaagaa agagaagaag aaattgcacg tatgctggct ggaagtgaga
    16321 ttacaaaact cacactcgaa catgcaaaag aactattgaa actcgcaaaa aggacctgag
    16381 ccacctcgca gtggttcagg tccttttcag cagctgatag ctatatttta tttttacatg
    16441 acactcatta cagcagtcaa tacaacagat ccaatcgtta aaatcagcat tgtccaaatg
    16501 acaaattttg ttactttagc aaaagtactt gtttgttttt tttcacgcat tgttttcaac
    16561 ctcgtcatta attagttgat tcaagtgtac aatctttttt ccatttccac aagagggctt
    16621 ttttataaaa acttcccttt ttttaaataa taggtagtta cgatacaaat aaataatgtt
    16681 aatagaacta gccatataaa ggcatcatcc ctttcagcaa taggtaactt aatgttcatt
    16741 ccatagatac tgccgacgat tgtcggtata gttaaaataa tcgttaaaga agttaaaatt
    16801 ttcataatat tattcagatt attcgatacg attgctgaaa aagtctgatt catttgttct
    16861 actagtttta attgaatgcg agaagtagtc attgcctgct ctgtttccac caatacatca
    16921 cgtaaatgat ttttcaaagc atgattttct tggaaaagtt tattttgcgc tagttctttc
    16981 aatgcatcta aattagcaga agtcgcttct ctgaaataaa cgaggctttt ttggatatcc
    17041 atgatttgat aaagttgttt gttttcagtg gatacttgaa gtttggcttc caaagtatcg
    17101 cattgagatt gaagcgtagc taaataactg ttgtaagaga gtgtgacctg ccaaatgaat
    17161 tgaaagaaga tattgagatt aggcgaaata tcactttttg gaaaggtctg gctaaagact
    17221 ttctttaaaa acagtggttc atgattcgta attgtgataa gcttcttttt gggcgtcaaa
    17281 ataatagaga gcgggtatgt atccacttga aaataaccgc taggacttgt tttagcaaaa
    17341 ggatattgta acaataataa cgctggcttt gtcaactttg attgattgaa ttcttctgta
    17401 cgggaatttt ctgcatcgtc caatatggaa gtgatgtaat ctgacggaat ttcaaattga
    17461 tgtgtcagct gatgaatctc ttcttgatta ggacgttcta cacaatacca gatcgtttgg
    17521 tcagtttcct ttccggtatt acgcagtttc tcattatcta atgtaaaata tttgatcatg
    17581 attttcctcc tgttgcctgt ttcttttatt ataacggata gggaattgga atacatggaa
    17641 tgtgtttgta agatatagaa tataactgca taaatttaaa taaaatgaaa cagttgtgta
    17701 aagtatatac agtatacttg aagcaacgat aaaaaaggag tttacgttca tgggcacatt
    17761 cacaccactt atcttgtttt ggtatattta cccggtaatc gttttgtttg gttgccaatt
    17821 tcttgtaaaa ttattttcgt taaatcgtcg ttttaaaata aaagcccctg atttggcgat
    17881 tccttttttg tggggcggta tgcatgcatt gtctagaaat acagggacaa tttctatctt
    17941 accgtttttc tttatttctg ttttgctaat gggaatttgt attgctgttt tccaagcata
    18001 ttattatgaa gaaattattt atccgcgtta tttcaagatg acttggcgtt tggtttttct
    18061 gttgacaatc gtcttgtacg ctgtcttgat cattgtgaac atactttctt acctatgaaa
    18121 aaaatagaaa agctgtaaaa agtcttttaa aaggcttttt gcagcttgat ttttaaaaaa
    18181 atgtttatga ataaaaaaag ttgttcccgg atttcccaga aaaggtggta gaaagtgggg
    18241 gattgtggta gactaatttt agttagtgga ggaatggggg gctttcaatg ttgatgggtg
    18301 aattccaaca taatattgat gcaaaaggac gtttaatcgt gccctcaaaa cttcgtgaag
    18361 aacttggcga aaagtttgtt ctgacacgcg gattggatgg ctgtcttttc ggctatccga
    18421 tgagcgaatg ggaaaatctg gaagctaaac tgaacgaaat gccgttggct aaaaaagacg
    18481 cacgtacttt tgtgcgattc ttctattcgg ctgccactga atgcgaactt gataaacaag
    18541 gacgaatcaa tatacccagc acgttaagaa attatgcagc tttgacaaaa ggttgcgtag
    18601 tcatcggtgt atctaaccga attgaaattt gggatgagac acgatggcaa gatttttctg
    18661 cagcagcaga agagaacttt gacgaaattg ctgaaagtat gattgatttc ggactataag
    18721 aataggaaga ggctgactaa tgactgaaac atttcaacac tatacagtca tgttgaaaga
    18781 aacggtagat ggattgaaca tcaaaccgga tggcgtttat gtagactgta cactcggcgg
    18841 tgccggacat agtgaatatt tattaagcca actaagttct aaagggcatc tgtatgcgtt
    18901 tgaccaagat caaaaagcaa tcgatcacgc caagctgcgt ttagcttctt atgtcgaaca
    18961 aggtatggtc acatttatca aagcgaactt cagagaatta gaagaacgct tgagtgagca
    19021 tgtagataag gtagacggca ttttgtatga tctaggggtt tcctctcctc agctagatga
    19081 agcagaacgt ggatttagtt atcatcaaga tgcaccgttg gatatgcgca tggatcagtc
    19141 agctccttta tccgcttatc acgtaattaa tgaatattcc taccatgaat tagtaaagat
    19201 tttcttccgc tatggcgagg agaaattttc caaacaaatt gctcgtgaaa tcgaacgagt
    19261 acgaaaaaca aaaccaatcc aaacaactgg tgagcttgtc gaaatcatta aacaagtcat
    19321 cccagctccc gcaagaagaa aaggcgggca tcctgcaaaa agaatttttc aagctgtgcg
    19381 gattgcagta aatgacgaac tcggtgccga agaggcctct ttagaacagg caatccggtt
    19441 attgaaagtc aatggaagaa tcagcgtaat cacattccat tcattggaag acagaattgt
    19501 caaaagtatg tttaaagaat atagtacggt acaagatcta ccaccgggac ttcctgtcat
    19561 acctgacgaa ttccaaccag aactgaaagt cattactcgt aaaccaatcc ttccttcaga
    19621 ggaagagcta acagaaaaca atcgatcacg cagtgcaaag ttacgtattg cagaaaaaat
    19681 aagaatgaaa gaggagtaga atgatggcag aattaaagaa aatgcaagat tacgaatatg
    19741 atttacctga attggtggat cagccggaac aacaaatgcc tgattcttct tcgaatcaag
    19801 aaatcattct tccacaatca ccggcaaaac gattaaagca gatttctgct ttggaaaaaa
    19861 ttgctgtggc aagcgtggcg attgctattg ttgctttatg tgttttaaca gttatgctgc
    19921 gcacaaatat cagcggagct gaaaaagaaa tcacgtcaat acaaacagaa atcaatgata
    19981 aaaagcaaga aaaaacgagt ttagaacaag aaaaaaacga actttctcgt actgaacgga
    20041 ttaaacaaat cgcagaagaa aaaggattgt ctattaatga cgacaattta aggaaagtga
    20101 agtaaatgag tcttaaaaat aaaattcggc ggtttatgga aaagaaaaat ctaaatccga
    20161 tgaacaatcg caaaaaagta ggtatcattt tatttgctac gagtattgga ttgttctttt
    20221 tatttgccgt ccgtttctcg tatatcgtta ttggaggtca tgttgctggt acctcattag
    20281 ctgaaaaaac aaaacagttg taccaaggaa gtgaagtagt aaaagcaaaa agaggaacga
    20341 tttatgatcg taacggtgtg gcgctcgcag aagatgcctc ctcttattca attaaagcga
    20401 tactctcaaa aacatatacc tctggggata aaaaactgta tgttgaagaa aagaattttg
    20461 ataaaatagc agaaatcctg cataagaatc tttctataga caaaaaagat gcactgaata
    20521 ttttagagga cggcgcaaaa aaagagctgt atcaagttga atttggtagc tatgggaaaa
    20581 atatctccca agaaaccaag caaaacatcg aagcggacat gaaaaaagaa ggtgtcgctg
    20641 gtctttattt tgtcgaccat caggctcgga tgtatccaaa tggcgtcttt tcttctcatt
    20701 ttattggata cgcagttcca gacaaagatg agaatggctt agttggtaaa ctaggtttgg
    20761 agtctgctta caatgatatc ttgagcggta aagatggtaa gattatttat caaaaagata
    20821 attttcaaaa tcctctgcca ggtacagtgg cagaagaaga aaaagctgtc gacggacaag
    20881 atatctacac gactttagac agcagactac aaagttattt agaaacactg atggatcaag
    20941 taaatgaaga atatcagcca gaagaattga cagcagtcct gatgaaagca aaaaccggag
    21001 aaatcttggc aatgggacaa cgacctactt tcaatcctga aacaatggaa ggattgactg
    21061 gaaaagatgc tatctggcgt aatttcttgg tacaagatag ttatgaacca ggatcaacca
    21121 tgaaagtttt cactactgca gctgctatcg aagaaggaga attcaacgag aatgaaacct
    21181 tccaatctgg aaaaatccaa gtagaagatg ctacgatcaa tgaccatgac tttggtgaaa
    21241 aaggtgtgct gaccatgcgt caagctcttt catggtcaag taacgtcggg atggtcatac
    21301 tggaacagcg attaggcgga cgatggtata actatttaca aaaactggga tttggacaaa
    21361 gtactcattc cggattagat gatgaagtaa acggtgcttt acctacatta aatattgtcg
    21421 accgagcaat gagtgcttat ggacaagcgg taggtgtgac gaatttccaa atgatgaaag
    21481 ctttcacttc tattgcgaat aacggtacaa tgattcaacc tcgatatatc agcaaagtgg
    21541 tggatcccca aactggagaa gagcgaacaa ctcaaacaga agttctaggg caaccttttt
    21601 caaaagaaac aacagaaaaa gtccgtgaat atatgagaga tgttgtcgaa agcgaaaact
    21661 acggtagtgc ttatggtgtt gtacctggat acaatgtatc ggcaaaaacc ggaacagccc
    21721 aaatcgcttc tgatacaggg ggatatcaaa caggagatac agcctacctg tattcgattg
    21781 tggagatggt tccttctgaa gatccagact atgtcttgta tctaacaatg aaacacccta
    21841 agacatatga tcgaatggca ttggctaaaa tcgcaaatcc attgatgaaa cgggcaatgg
    21901 attttaaaga aactgaagaa gattcagata cagaaacaaa aacagaaaaa gtatccgtag
    21961 ccgattatcg taatcttgaa gcggacgttg cagcagctga tgctcaaaaa agtggactgc
    22021 aacctgtagt gatcggtaat ggtaaaaaag tacaaaaaca atctactgcc aacggtgatc
    22081 agttgatctc tggagaaaaa ctgatcctat atactggtgg tgataagctg atgcctgacg
    22141 taacaggatg gtcaaaagca gatatcatga agctggggaa aatcctcggt attgaagtta
    22201 gttttgatgg agatggttat tgtgtcaaac aagaacttgc tccatatgaa aaaatcactg
    22261 aagacaaact tagttttact ttagaagaat aagaagagaa ataggagaga aaagagcatg
    22321 gaatggacac aagcattgat tcccattgtc agcagttgtg caatgacaat tgccgcaatg
    22381 ccgctattta ttggttattt tcaaatgaaa aagcaaggtc aggcgattcg tgaggaggga
    22441 ccaaagtggc acaatagcaa agccggaact cctacaatgg gcggcttagt gtttctgatc
    22501 ggctctattt tgacaggaat ttgggttgga gcttggcaaa aacagctaac tcctactctt
    22561 ttcatcttgc tttttgtatt agcgttatac ggagtaatcg gctttcttga tgattttata
    22621 aaaatattca agaaaagaaa catgggactg aactcgaagc agaaattgat tggacagatt
    22681 ctagggggaa tcatctttta tcttgtttac cgttctgaag gttatccagg tgtattgaat
    22741 ttcttcggac tagatctacc tttaggaatc atttatggta ttttcgctct cttctggctt
    22801 gttggatttt caaatgcagt aaatctgaca gatggcatcg atggattagt tgctggctta
    22861 ggaagtatct cttttgcagc ttacggtcta attgcttggc atcagcatca gtatgatgta
    22921 ttaatcattt gcttaagtgt tttaggaggt ttactgggtt tcttcgtcta caacagaaaa
    22981 cctgccaaga tttttatggg agatgtcggt tcattagcgt tgggcggact tttagcggca
    23041 atttctataa tgctaaacca agaatggaca ttgcttttga ttggtttgat ctatgttatg
    23101 gaaacagcaa gtgtgatgct tcaagttact tcctttaagc ttacaggaaa acgaattttt
    23161 aaaatgtctc caattcatca tcactttgaa atgtgcggat ggtcggagtg gaagattgat
    23221 acagtttttt ggttgatcag cattgtgact tcgttgatca cattatggat cgtttggtag
    23281 gagatgacgg gaaatgaaaa aaatatcaac atatgaaaat aagaaagtgc ttgtcttagg
    23341 actcgcaaaa agcggagtaa gtgcggccaa attattacat gaattgggcg cacttgtcac
    23401 cgttaatgat ggaaaacctt ttgacgaaaa tccggaagca caagagctgc tttctttagg
    23461 gataaaagta ataacaggta gtcatccaat cgaattgttg gatgaagaat tttcattgat
    23521 ggtcaaaaat ccagggattc cctacagtca tcctttagta gcaaaagccc aagaaatggg
    23581 tattccggta attacggaag ttgagctagc ttacgaagta tcggaatgcc caattattgg
    23641 gattacagga acgaacggaa aaacaacgac aacaacgatg acaggactac tactgaatgc
    23701 aggagcagat caaggaattg cacgtttagc gggtaatatt gggtatccag caagtggtgt
    23761 agcgcaagaa gcaaaatcag aagataaaat tgtgatggaa ttgtctagct tccaactcat
    23821 gggaatcaca gatttccgcc ctcatatagc ggtgattacc aatatctatg aagcacatat
    23881 tgattatcat ggtacaagaa aagaatatgt caaagctaaa tggaaccttc aaaaaaacat
    23941 gacagaaaaa gattatctga tccttaactg gaatcagtca gaactacaag aattagcgca
    24001 aagaacaaaa gcacgcgtac tgcctttctc gacaaaagaa gtgttggaag acggtgtgta
    24061 tgctgacgat tactcaatat actataaaaa agaaaaaata atggaaatat ctgaacttgg
    24121 cgtaccaggt aagcataatg tagaaaatgc actagctgct atttctgtag caaaattgta
    24181 tggtatatca aatgaagcta tacgagaaac gttgcatttt ttccatggcg taccacatcg
    24241 tacgcaatat gtaggagaaa tccaaggcag aaaattttat aatgattcaa aagcaaccaa
    24301 tattttagcg actaaaatgg ctctaagtgg atttgaaacg tctaaggtcg tgttattggc
    24361 tggcggatta gacagaggaa atacatttga tgaattaatt ccttcattaa aaggaatcaa
    24421 agcaatggtc gtatttggcc aaacaaaaga aaagttgatg gatgctggaa aaaaagcagg
    24481 catcgagaca atagtaacag ccgattctgt agaacaagca gttcctttag cattagaaaa
    24541 cagtacagat ggtgatgtag ttttattatc accagcgaat gccagctggg atcaataccc
    24601 aaattttgaa actagaggaa acagattcat ggaagctgtg aaccgattaa aatagaaaaa
    24661 gtagaagcga gcgataaaaa atgaaaattt tggttacagg cggaggaaca gggggacata
    24721 tttatccagc tcttgccttt gtcaattatg taaagactca agaaccgaat gctgaattca
    24781 tgtatgtggg agcaaaacga ggtctagaaa acaaaatcgt ccctgataca ggaatgcctt
    24841 tccatacctt ggagattcaa ggatttaaac gaaaaatttc catgcataac atcaagacgg
    24901 tccgattgtt tcttaagagt atccgtcaag caaaaaaaat cctcagtgat tttcaaccag
    24961 atatcgttat cggtactgga ggatatgttt ccggagcggt agtgtacgct gcagccaaaa
    25021 tgggtattcc aacagttatc catgaacaaa acagtgtacc aggtgttacc aataaatttt
    25081 taagccgata tgtggatcgg atcgctcttt catttaaaga tgcggcttcc ttttttcctg
    25141 aaaacaaagc agttcttgta ggtaatcctc gagcacaaga agtagcagat actaaaaaat
    25201 cagatatttt gaaaacattt gatttggatc cagaaaagaa aacagtcttg atttttggcg
    25261 gtagtcaagg agcacttaaa atcaatcaag cagtatcagt ttttttaagt gaatatattc
    25321 cttcttctta ccaagttttg tatgcttcag gagatagata ttatgaagag atcctaaata
    25381 aaataaatcc tgaaatacaa aatattagtg tccagcctta tatcaaaaat atggcagagg
    25441 tcatggcttc cagtgatctg ctggtggggc gtgctggtgc aacttcgatc gcagaattca
    25501 cagctctggg gttaccggca gtgttggttc ccagcccata tgttacgaat gatcatcaga
    25561 ctaaaaatgc aatgagtctt gttcacgcag gagcagcaaa aatgattgca gataatgaac
    25621 taacaggtga atcactaagt cagacagtca acgaaatcat gggtgatgaa gaattgcaaa
    25681 agcaaatgtg ccgagcttct aaagaacaag gaatccctga tgcttcaaaa cgtctttatg
    25741 acttagtaaa acaaataatc aaaaaataaa ggcggtggtc ttagtgagta agaaaaaaga
    25801 accaaaaaaa ttgtctcatc cgtcttctga tgaaaacaat ttgactcctt ggcaaaaggc
    25861 taaccgaaaa tatctggcag aacaagagat gaaaaaccaa gagccaaaaa aagaagacat
    25921 aacattagat acaccccttt cagattctga agaagaaaaa acggttcaag aggaacagca
    25981 agaagaaagc cagtccgaga aaaaaatcga attttttgaa gagatcaagc aaaagaaaaa
    26041 cggtggacca tataacggtt cttttttgaa ccggctgccc aatttaaaaa gtcaacggca
    26101 caaagtacta tttcgtcggt tagctttgat catcactgta ttgggtatac cgctgatttt
    26161 tttgatctat tatgtttctc cttatagcaa attacaagca gttgcagtat caggcaataa
    26221 gacagtcaac agccaagaaa tcatctctga tacaaaattg tcattaggag aaaatgtttg
    26281 gggacagtat tttcatcgat ctacttatat cgatcgcctt aaaaaagcac agccaagaat
    26341 cgaaacagca aatatacatt tcaaagggat gaatgaattc gatttggatg taacagaata
    26401 taaagagatt gctttgatcg caaaaaacaa tcaatactac ccagtgattg aaaatggaac
    26461 agtattagat gaaaaagtag caaatcctac aaaaaatctc cctatcttgg aagagttcaa
    26521 agactctgca aaaatcaaag aattaacaaa gcaatacaat cagttatcat cagaattaca
    26581 aaaagcaatt tctgaaatca agtacacgcc aagagcaagc aataaaaatc tgatccaatt
    26641 gaatatgaat gacggcaatc aagttattgt aaatatcaat aatcttgcga accaaatgaa
    26701 atattattcg caagtagcca aagatatgga tgaaaaaggt gtgatcgaca tggaagttgg
    26761 tatcttctcg catccttacg gtagcactca agaaacggaa gaatcgggcc aatcggaaga
    26821 aacaactgaa agtgaagaaa atgcggatca aacaaacgaa tcaagtgaag aggcagaaac
    26881 gcaaaaagca caaacacctg gatcgtctag cacaaacgga tcatctgatt caaatgattc
    26941 aagtgaaaca agtcaaatag aagataatcc aagtacaact ccgtcaagtt taaatgaaaa
    27001 ttgataaaac aactttaaaa agtaagaatg tactttctaa aaaccagtaa tttatggtag
    27061 aattggaagc agtgaatata tctattaata agtatatttt aaaacggacg ctagttgttc
    27121 gcttgtatat ggaatagtaa gtaggaggga atcccattca tggcaaaaac aggaatgtat
    27181 gtaggccttg atattggaac gacatctgtt aaagttgtcg tggctgaata tatcgacagc
    27241 caaatgaata ttattggagt aggaaacgca aaatcagaag ggattaaccg aggcattatc
    27301 gttgatatcg ataaaacggt ccaagcgata caacgagcag tacgacaagc agaagaaaaa
    27361 gcaggtatcc agatcaaagg cgtaagtgtc ggtttgccag caaacatgct tgaagtagaa
    27421 aattgccagg gaatgattgc agttaacgga gattcaaagg aaatcaccga tgaagatgtc
    27481 agaaatgtgg cttcagctgc ccttgttcgt tcaatacctc ctgaacgtca aatcgtgtca
    27541 atattgccac aagattttac tgtcgatggg ttcgagggaa tcaaagatcc tcgtggaatg
    27601 atcggcgtaa gattagaaat gtacggtctg ttgtttacag gacctaaaac gatcatccat
    27661 aacatacgta aatgcgtgga aaatgccgga ttaatcgtca acgaaatggt gattactcct
    27721 ttagcgctga ctgaatcaat tctttctgac ggagaaaaag attttggtac gatcgtcatt
    27781 gatatgggtg gcggccaaac aacaacggct gttatgcatg ataagcaatt gaagtttacc
    27841 aatcttgacc aagaaggtgg cgaatttgtc actaaagata tctctattgt attaaatacg
    27901 tcatttaaca atgcagaagc gttgaaaata aactacgggg atgcataccc agaacggaca
    27961 tcaccagatg aagaatttcc agtagatgtg attggtcagt cagaaccagt gaaagtcgat
    28021 gaacgctact tgtcagaaat tatttcagct cgaatggaac agattttcaa caaagcaaaa
    28081 gatgcattgg accaaatcga agcattagag ttgccaggtg gcgtcgtatt gacaggcgga
    28141 gctgctagtc ttcctggtgt tgtggacttg gcacaagaaa tcttcggcgt aaatgtaaaa
    28201 ttgtatgttc caaatcacat ggggctgaga aacccggtat ttacaaatgt aataagtatc
    28261 gtggattatt cagcaaactt gagtgaagtg tatcaattgg caaaaattgc tgttacgggt
    28321 gaaacctcag cagctcgtca aatggtagtt gaacaagaaa caacaaacac atatgagagc
    28381 tatgaagcac ctgaagaaac aatctacgat gagccggaac caaaagagtc cggagaaaat
    28441 gtaaaaaata aaatcaaggg tttctttaca aatatttttg attaattcat cctgggagga
    28501 ataagatatg gaattctcaa ttgataataa tatcaatgac ggcgcagtaa tcaaagtcat
    28561 cggagtcggt ggtggcggtg gaaatgctgt aaaccgaatg attgaagaaa acgttaaagg
    28621 tgttgaattc attacagcca acacagacgt gcaagcatta aaaaattcaa aagctgaaac
    28681 agttatccaa ttaggcccca aatatactcg tggtttaggt gctggttctc agccagaagt
    28741 tggacaaaaa gcggcagaag aaagtgaaca atccttacgc gaagctttag acggtgcaga
    28801 tatgattttc atcactgcag gaatgggtgg aggaaccggt acaggtgctg caccaatcgt
    28861 tgcaggtatc gcgaaagaat tgggcgcatt gactgttggt gtcgtgacac gtccattcac
    28921 ttttgaagga ccgaaacgtg gccgttttgc tgcagaaggt atcgctcgtt tgaaagaaaa
    28981 tgtggataca ttattaatta tctcaaataa ccgtttatta gaagttgtag ataaaaagac
    29041 accgatgctt gaagctttcc gtgaagcaga taatgtttta cgacaaggtg tacaaggtat
    29101 ctctgatttg atcacagcgc caggttacgt taaccttgac tttgctgacg tgaaaacagt
    29161 gatggaaaac caaggtacag cattgatggg gatcggtgtg gctagtggag aagatcgcgt
    29221 agtagaagca acgaaaaaag caatctcctc tccattgctt gaaacatcta ttgatggagc
    29281 tgaacaagta cttcttaaca tcacaggcgg tttggatatg acattgttcg aagcacaaga
    29341 tgcttcagat atcgtagcaa atgcagcaac tggcgatgta aatatcattc taggtacatc
    29401 tatcaatgaa gaaatgggcg atgaaatccg tgtgacggtc atcgcaacag gtatcgatga
    29461 atcaaaaaag gaacgcaagt ctagccgacc agctagacaa gcacaaatgc aatcacctgc
    29521 tcaaaagact gtattagata tggatcaagc taaaccaact tcttcagaag aagaaaacag
    29581 ttttggcgat tgggatatca gacgtgaaca aaatgttcgt cctagagttg atgattccaa
    29641 ttttgacaat atcgaaaaaa aagaatttga tacattcaac cgcgaagaaa caaaatctca
    29701 tggcgatgat gaattaaata cgccaccgtt tttccgtcgt aaaaaataaa ggagaggaag
    29761 agcacatgat tgctgacaac ttgcagaaaa tcaagcaaga aattcaggat tcgtgtgctc
    29821 ttgttcatcg tgatccgaaa gaagttacat tagtagctgt aacaaaatct gttgaatccg
    29881 ctcaagctga agaattggtt agagctggcg ttatgaattg tgcagaaaac cgagtagata
    29941 aattattaga aaagaaacaa gatttatttg attttcctga aataaagtgg catttaattg
    30001 gtaatttaca gcgcagaaag gtaaaattga ttgtaaatga aattgattac tttcatgcgt
    30061 tggacagttt aagattagca gaagaaatcc agaagcggtt agagaaagaa ttatcctgtt
    30121 ttattgaagt aaatgtttct ggtgaggaat caaaacatgg aatttcacca gatgagcttt
    30181 taccgtttgt ggaatcttta gctgcctgca caaaaatcaa ggttgttggg ttgatgacta
    30241 tggcgcctaa agaagcttct gatgagcaaa tccataaagt gtttggtacg ctgaaagaat
    30301 tgcgtgatct tgttcaagca aaaggttatg cccatgcacc atgtagtgaa ttaagtatgg
    30361 ggatgagtca agactttcaa attgcgattg aagaaggtgc aacatttatc cgtgtaggaa
    30421 cagcgctatt caaagacgaa taaaggagga acaaaaatgc cgcttttaaa cagaggagca
    30481 attgctaatt ttttcggatt agctgatgag gaagagtacg aataccaaga ttatcctgaa
    30541 acaacaaaaa aggctgtaca gccaactaaa aaaaatacaa cacagaatca atcaactcat
    30601 tctgtatctg ctgcgaatac tcatgaaaaa caaaaaacgg cgacttcacg tcctgtaacg
    30661 catgaaagta ttcagccggc tgaaaagaaa aaagtaattg aactacatca atcacctaca
    30721 aaaactgtag cgaaagaaag aacagcttca agaacctcta cacaacctaa agcaaatctt
    30781 gctggaaaaa tcactgtaat ggaaccaaga gcttattctg aagctatggc gattgctaaa
    30841 cgaatcattg ctggagaagc aaccttagtg aattttcatt tagtagaaga aaaacaagcg
    30901 agaagaatcg ttgattttct aacaggaaca gtttatgcac tagatggaga tatcaaacgt
    30961 gttggagatg agatttttct ttgtacaccg actgggatgg agattgacag ttcaaccgct
    31021 caatcttttg cagatgcgaa tttgtttgat ctgtaagagg aggaactacg atttatacta
    31081 ttgcactatt acttcattta cttcaacggg cagcctattt ctatagcatt atcttgatgg
    31141 tatatgcatt gctctcttgg tttccaggcg ggtatcaatc agggctagga cgattattag
    31201 ctaagatctg cgaaccgtat atcagtttgt tcgatcgtct gcctttgagg attggaccct
    31261 tggatttcac aattttaggg gcaatcctga tcttgaactt agccgtacaa ggtttagcac
    31321 agatcgttat atggataact gtccatttag tttattgatt gaagcaacaa aatctttatt
    31381 ctcagaatag ataggagggg aaaattttgg atgcaaatgt ataccagcac tttcgggcag
    31441 atgaacgtac atttatcgat tcggtaggcg attggattga acaggttcaa ggacaatatg
    31501 caccctatct gaccgaattt cttgacccaa gacaatccta tattttagaa acactgattc
    31561 gacaaagcag tgatttgcgt ttcatgtttt atggcggata tgaacaggca gaaagaaaaa
    31621 gatgtttgat tttccccgat tattacgaac ctgtagagga agattttgaa acagcattgt
    31681 atgaaatcca ttatcctagc aaatttgcca gcctaagtca tgggaaaatc cttggaactt
    31741 tagtgggaac ggggatcaaa cgctcttact ttggcgatat catctcggac ggtgatcgct
    31801 ggcaagtatt tattgcaaat gaggtggatc attttgttgc tgcacaagta acaaagattg
    31861 gaaaggttgc tgtacgcttg gaaccaatca aatacacaga aatgattatg ccgaaagata
    31921 gctggacaca agaaagagga acagttagtt ccatgcgact agacagtgtg atctctgaga
    31981 tttataatat ttccagacag cggtccaagc agctgatcga agctggtaaa gtaaaagtga
    32041 actggacaga aaatacaaga cctgattttg tacttgaact attagatatt atttcagtca
    32101 gaggttatgg cagagtccag atccaagaac ttgaggggaa gacgaaaaaa gaaaagtaca
    32161 gggtactgtt cggtgtatta cgtaaataaa ataaaaaggt ggtttactca tggctttaac
    32221 tccattagat atccaaaata agacattccc gacaaaaatg cggggatata atcaagacga
    32281 agtagatgat ttcttagatt tagttgttcg tgactatgag gaattgactc aaagaaatcg
    32341 agaattagaa aaagcagtca aacattctga agaaaaactt gagtacttca atgaattaaa
    32401 agatgctttg aaccaatcaa tcatcgtagc tcaagatacg gctgataaag taaaaacaag
    32461 tgcaagcaag gaatccgaag tgatcgttac ttctgcacaa aacaaagcag atgagttagt
    32521 tgcgaacgct gaaaaacgtg cacatcaatt aacaactgat gcagaagaaa aagcaagaaa
    32581 aatcttgaca gatgcaacag aaaaagcaag acagcttgcg acagaaactg aagatcttaa
    32641 gaaaaaaaca cgagtattcc atcaacgtat cagcttgatg cttgagtctc aattagaaca
    32701 agtgaaaagt ccggaatggg atgaaatttt acaaccattc tccagctacg tgactgacag
    32761 ccatgaagtg atcaaagaag ttttagcaaa acagcttgac aatgaaaatg atactgatgt
    32821 aaactcagac acaggagtca tagaagcacc tatgacggca tttgatacac aggctattga
    32881 tatctcgatc gtgcctggtg aaaatgaaaa cgagtagaac agaaaagcac tttgttattc
    32941 ttaaagcgag ccgatgagag tggaagttcg gtaagacact gcatggtgaa agatcctcta
    33001 cttgagacct gcttttccga aattagagta agaagcagcg gctgacctcg ttattggtca
    33061 cagagagttt gcactagata gatgcaacta aatttgggtg gtaccacggc atgttcgtcc
    33121 cttgcggctg aacatgcttt tttgtgtccg gaaaacaaat aaaggagtgt atggttgaat
    33181 gaaaatgaaa gaaaccttgc aattaggaaa aacaaaattc cctatgcgtg gtaatttgcc
    33241 aaaccgagaa ggcgagtggc aaaaagaatg ggaagaaaat cgaatctacc aaaaaagaca
    33301 agagttgaac gaaggaaaac ctagctttgt tcttcatgat ggacctccat atgccaatgg
    33361 taatatccat ttaggacatt cattgaataa aatcagtaaa gacattatca ttcgttcaaa
    33421 atcaatgtct ggctatcgtg caccatatgt tccaggatgg gatacacatg gattgccgat
    33481 tgaacaagta ttggctaata aaggcgtgaa acgtaaagaa atgtccatgg cagaatactt
    33541 ggaaaaatgt cgtgaatatg cactttccca agtagataag caacgagcag atttcaaacg
    33601 cctaggagtt gcaggagatt gggaacatcc ttatgttaca ttagatcctt cttatgaagc
    33661 tgcacaagta cgcgtatttg gtaaaatggc tgaaaaaagc tatatttaca aaggattgaa
    33721 accaatttac tggtctccgt caagtgagtc ttctttagcg gaagctgaaa ttgaatataa
    33781 agatgtaaaa tctccatcta tttttgtagc tttcaatgtc aaagacggca aagatattct
    33841 tgatgaagat acagcttttg tcatttggac gacaacacca tggacattgc cagcaaacca
    33901 aggtattgct gtaaatccaa aatttacgta tgtattggtg gaagccgatg gccgtaagtt
    33961 tgtcgtagcg aaagatttgc tagaaacagt ccaatcagaa atcggttggg aaaatgtaac
    34021 agttctaaaa gaatttgctg gacaagaaat ggaatatatg actgcaagtc atccattcta
    34081 tgaacgtgaa tcacttgtga ttttaggcga tcatgttact ttagacgctg gtactggatt
    34141 agtccatact gctccaggac atggggaaga tgactatatc gctggtagca aatataaact
    34201 atctgtagtg tcacctgtag actcaaaagg attgtttaca gaagaagcac ctggatttga
    34261 aggtatcttt tatgacaaag caaatcctat gatcactgat ctattaaaag aaaaaggagc
    34321 tttgctgaag ctagatttct ttactcatag ttatcctcac gattggcgga cgaaaaagcc
    34381 tgtgatctat cgggcaactc cacaatggtt tgcatctatc gataaattcc gtcagaatat
    34441 tttggatgaa gtagaaaaag tagagtgggt tatcccatgg ggtaaaacac gtttgtataa
    34501 tatgatccgt gaccgcggcg attgggtaat ttcccgtcag cgtgcatggg gagtaccact
    34561 tccgattttc tatgctgaaa atggagaagc aatcatcaca ccagaaacaa tcgaacatgt
    34621 ggcaaaatta tttgctgaac atggatcaat tatctggttt gaaaaagaag cgaaagaact
    34681 tcttcctgaa ggatttaccc acccaggttc tcctaatggc gaatttacaa aagaaaaaga
    34741 tatcatggat gtctggtttg attctggttc ttctcatgaa gctgttttac gtcagcgacc
    34801 agaattatca ttcccagcag atatgtattt agaaggatca gaccaatatc gtggctggtt
    34861 caactcaagt atcacgacta gtgtggctat caatggtgta gcaccataca aatcagttat
    34921 ttctcaagga tttgctttag atggtgaagg ccgtaagatg agtaaatctt taggaaacat
    34981 cattgcacca gaaaaagtga tcaaacaatt tggtgcagat atccttcgct tatgggtatc
    35041 tagtgtagat tatgaagcag atgttcgtgt ttctatggat atcttaagtc aagtggcaga
    35101 agtatatcgt aaaatcagaa atacgatgcg tttcttatta gcgaatacag aagatttcga
    35161 tcctaaaaaa gatactgttt cgtacaatga tttacgttct gtagacaaat atatgaccgt
    35221 tcgattgaac caagtaatca aagaaatccg agaagaaggg tacgacaaat ataacttcat
    35281 gcatatttac cgtacagtta tgaacttctt gacggttgat ctatcgtcat tctacctgga
    35341 ttttgctaaa gatgtagttt atattgaagc agaagatgat tatcaacgtc gttgcatgca
    35401 aactgttttc tatcaaacag ctgtagcttt gacaaaatta ttgactccta tcattccgca
    35461 tacagcagaa gaaatctggt cattcctgaa agaagaagag gagtacgttc agttaagcga
    35521 gtttcctggt tatgaagagt ttgcaaacca agaagaattg atggatacct ggactgcttt
    35581 catggacttc cgtgataatg tcttaaaagc attggaagaa gcaagaaaca gcaaattgat
    35641 tggtaaatct ttggaagcaa aactaactgt ttatccaaaa caacaagttc gtgaaatgtt
    35701 aaaagctttg gatgcagata ttgcacaatt attgatcgtt tcaccagact actttgaagt
    35761 tatggcagca gatgaacaag tgccagataa cgctatggta tttgatgatg ttgctattac
    35821 agtggaaaaa gcagacgggg aaacttgcga tcgctgtcgc caagttcgta aagatgtagg
    35881 agtagatgaa aaacttcctc atctatgcgg tcgttgtgca aaaatcgttg aagacttcta
    35941 tcctgaagct gtagctgaag gattcgaaga aaagtaaaag ggaaaagggg gtgtgacatg
    36001 ttctttgtca cacccccttt tgttatcaat cgtttaattt tccacgttca cgataccagt
    36061 tatctggttg ccagatccat tcgaatccgt ctttttcaag tagttcgaaa gctgctttag
    36121 gtcccattga tccagctttg tagttgggaa aatccggttg tgtttggtcc caagcttttc
    36181 gaatgacatc cacgatatgc caagattgag cgacttcttc ccaatgagag aagttcgttc
    36241 catcaccatt caacgcatct aatagtaatt tttcgtaagc ttctgggcta ttttcaacca
    36301 tttctgcact atttctaaat tccaatttga ttggttccgt ttcaaatcct tgccctactt
    36361 ctttcccatt caaggataaa gaaaatcctt cagttggctg aatatagatc gttagaacat
    36421 taggtggaag tgtcgtatcg tcacatggtt catcgacaga tgttttgaat acattaacag
    36481 gaacttgttt gaatacgatg ttgatccgag tccctttttc agtcaaacgt ttgcctgtac
    36541 gtacatagaa aggaacacct gaccaacgga agttatcgat agtgaatttg cctgcgacga
    36601 atgtttcagt ctctgattga gcatccactt tgtcttcttc gcgatagccc ttgaattgta
    36661 caccatccaa ttcaccttga gcatattgcc cgcgaacgaa gttttctaac acttcttctt
    36721 cgttataaat gcggatagct ttcaatgctt tgatcttttc tgtacggatt tctttttcgg
    36781 aaaatgctac tggcggttcc attgcaagta atgctacaac ctgtaaaata tgattttgca
    36841 ccatgtcttt aagtgcgcca ctatgatcgt agtagccccc gcgatcttct acaccgaggt
    36901 tttcgccaaa agtaatttgg atattgtcga tatagcgatt gttccattga gcctcaaaaa
    36961 tattattggc aaaacggata gctgagatgt tttggatcat ttcttttcct aaataatgat
    37021 caatcctgaa aatatcttgt tctgggaata cttctctgat ttgctggttc aattcaaaag
    37081 cagactggta atcagaaccg aacggttttt ctatgatcaa acgattgaag ccattttttg
    37141 tcataatatt ttgtgatttc aaatgagaaa cgatcgttcc aaaaaattga ggtgccatag
    37201 ccaaataata gacacggtta ccgtttaatt gatagtgtcc atctaatcgg tcagacaatt
    37261 cttttaatgt ctgataatgc gttgaatcat ttacatcatg ggattgatag taaaaatggc
    37321 ttgcaaaggt agttgcttct tcatctgaag gattcaatgc acggattgtt tctttgacaa
    37381 cttcacggta gtgctcatcg ctccagggac ggcgagcggt tccgattaca gcaaaatgct
    37441 cgcctaaatt tccttttctg taaaggcgga acaaagacgg gtacaattta cgctgggcta
    37501 ggtcacctgt accgccgaat atcgtaaaca atacattttt ttcattagac atgtggatac
    37561 actcctttat attttatcga caaaaatcgt gctggccgcc ttaaagctga cggggatttt
    37621 ctttctatca ttttggatag tgatcggtcc ttcataagct gcgatgtcaa cgatctcata
    37681 ttcttcgttg atattcaggt caatagtgac taagtaatcc aacaattctt tttcatcaag
    37741 cactcgtgcg atacgcactt ttgtaccgac aggataggac tccaatgttt gtcggcgttc
    37801 ttcatggaca acttcctctt ttttaggaat cgatcctcca tgaggacaat gttcaggatg
    37861 attcaaatag ttttctaaat gatctgcaag atgttccgaa gtcacatgtt ccaatacttc
    37921 agcatcatca tggacttcat tccagctata gtctaaatgt tcaactaaaa aaacttccca
    37981 taagcgatgt ttgcgaatca gactgctagc tttttgcagt ccgcttgcag tcaattgtac
    38041 accttgataa ggggaatgtt ctacaagctc ttctttcaca agtttagaaa tcatttcact
    38101 gacagatgca gctgagacat ctagactaga tacgatttgt ttattgttga ctttcgttgt
    38161 atctccacct agttctaaaa tgattttcaa ataatcttct cgatttggtg tcatattcga
    38221 tcatcctttc atggtaaaaa accatcatta gtaagtgtaa caaaaaaaac gttaattgac
    38281 tatttttcta aataaaaaac aaaggatttt tagacgtttt tacaacaaaa aacgaaagat
    38341 gatagagaag actcattctt tcgtttataa aggatatata tatacattgg gagggatcta
    38401 tttttcagat aacaacttat atgaagtgtc tgcagattcg tcataaagag tttgttcggg
    38461 ataagaaaag ataagataaa aaccatactg atcacgtacg actaaacgat attcatctat
    38521 ttctaccaca gatgtgccaa gagactgacc gttgatcaaa aacgtgtaat cagatgtaat
    38581 ctgtaattgt tgtttttgct ggctatcact aaagaaccat gtaccttgat actttgagag
    38641 tccttctgac ttttccgctt gttttttctt tttcaagtaa ataaaacttg agagaacggt
    38701 aatgagtgaa ccaagaaaat agatcatttt ttttatcacc aaatcactcc tttatgagaa
    38761 aataatcgca tagacatatt ctttcttctt tatttcattg taccgaataa aagctaaaat
    38821 ttctctaaga ctttcgactg gtttttttac tgtttttcta caaattatga taggatacat
    38881 agtaggaaga aaggtgacat aatttgaagt atcagcaagt ggaaatagct ttttttattg
    38941 accgcccgaa tcggtttata gcacattgta tgaaaaaaga cggagaaata attattgcgc
    39001 atgtcaaaaa tacaggtaga ggaaaagagg tcctgatacc aggcgctgaa gtggctattg
    39061 tatttgctcc tggacctaaa cggaaaaccg cctatgatct gattgctgta aaaaagaagg
    39121 gtgactggtt taatatcgat agtcaattgc ctaatcggct ggcaattgat ggtattttag
    39181 acggcacgat ccttttacca ggattaactg gtccatttac ggtatacaag cgtgaagtaa
    39241 cttatcttca ctccaaattt gatatttatt tagagacggt ggtcggacaa aaagcatttg
    39301 ttgaagtcaa aggtatgaca cttgaaaata aagcgattgg tgcttttcct gatgcgccca
    39361 cactcagagg attgaaacat ataggagaat taacgtatgc cgcacaagat ggttatgccg
    39421 cttatgtctt gtttattgct cagttcgaac atcttcatct ggcaacgatc catgaagaaa
    39481 tgcaaccagc actagctgat atggttcgac atgcgcagca aagcggagtt caaatcctcg
    39541 cctataattg tcaagtatcg gcagatgagg taactgtcaa gcaggccatt ccatttgatg
    39601 tagatatccc ttttgaagat cctatacaat agaaagaagg taaaataatg atcaacttag
    39661 gtattatcgg aacaaattgg attactcatc aatttgtcca agcagcttta agcacaaaaa
    39721 aatatgaatt gacagcggta tattctagac atttggaaaa agcacaggaa tttggttctc
    39781 attatgaagg ggatatcgaa ttcgcaacag atttgaagac ttttttcaat attactcata
    39841 tggacaccat ttatattgct tctccgaata gtcttcactt tagtcaagct aagcaagcaa
    39901 ttttggcagg aaaaaatgtg atagttgaaa agccagcttt ttcgacgcca aaagaaatgg
    39961 atgaaatcat ccatttggca aatgaacaca atgtgtattt ttttgaagca gcaagaaata
    40021 tccatgaatc aggttttaaa aagattgccg attacttacc attaaaagac gagataatcg
    40081 gtgcaaattt cagctatatg aaatattctt cgcgctatga ccaagtatta gatggagaag
    40141 aaccgaatat cttctcgact catttctcag gtggtgcatt gatggattta ggtgtttacc
    40201 ttgtatatgc agctgttgct tggtttggaa tgccaaaaga agtccattat ttcccaagaa
    40261 agatttctac tggtgtagat ggtcttggat gggggatctt acgttatgag aattttgatg
    40321 tatcgatcca gccaggtaaa atcggtgatt cttatttacc gtcagagatt tattttgact
    40381 caggaacgct agtgatgaat ggagtcaatg caattgaaca agcacaattt catgatcgcg
    40441 cgcataaaga aatcattgat ttggcggtaa cagctgcaga aaatccaatg gcagatgaag
    40501 cagaggattt tgccaatgtc atggctcatc cgaaagatcc agcttgggga ttgttgtatg
    40561 aagagtggat cgagctagct agaaatgtga accaagtgat ttatgattta agaaaaaatg
    40621 gcggcatcaa attcgatgca gataatgagg aagattttta atgaacgaat ttgtacaagt
    40681 atttcccgaa aattggcaga aaaaatatga ggataaaggg tttatctcac cttctcttat
    40741 tcaacgagca gtttttcagc cactaagtaa taaacaaagt atagtagcgg tttctcctac
    40801 aggttcaggt aaaacactgg cttatctttg gcctctacta ctaaacgtgg aaccaggtga
    40861 agcaagtgcg ttggtcattt ttgcttcttc tcaagaacta gctattcaag tagcagatgt
    40921 tgcacgtgaa tggggaaaag acaaagagtt gaaagtccaa tctttggtgg gcggagccaa
    40981 cgtcaaacgt cagattgagg gtttgaagga aaaaccagaa atcttgattg gtacaccagg
    41041 aagaatattg gaattgatga aagcaaaaaa aataaaagca catcaagtga aaacaatggt
    41101 atttgatgaa gcagaccagc tatttgacgc tgggaacagc caaatcattg atcaaattct
    41161 tcatcaagct ccaacagagt atcagctagc ttttttttca gcgacagcag atcgttccct
    41221 tgaaagcatt gaaaaaatca ctgggaaaac gtttgagaca attgacgtga cagcagagga
    41281 cgattctcga aaaggactga gacattactt tttacgtgta ccgatacgaa agaaagatga
    41341 ctacttgaga cgtctcacgc atatagaaga ttttcatggt ctgattttct ttaatcaact
    41401 gactgaatta ggcgtaatgg aagaaaaatt gctgtataga ggtgtaccgg taggaagtct
    41461 ggcctctgat cagaacaagc tactccgaaa agcagcgcta gaacaattca aaaagaaaat
    41521 ggtcagagca cttttgacca cagatgtggc ggctagaggt ttagatatta caggtcttcc
    41581 ttatgtagtg aatgcagaag ttccgttaag tgaagaagct tatttgcatc gttcgggaag
    41641 aactggtcga atgggtaatg aaggaatagt aattacactc gtacaagata ataatctaag
    41701 agatttaaaa cggcttttac gtccatcaaa tataacactt gaagagattt ttttatacgg
    41761 aggtaagctg caaactgagc agccaaccaa agaagatgca gctcctgcag taaataaaaa
    41821 gtatgcgaaa actttttctg gaaaggagca gaacccagaa tataacaaag aaaaaataag
    41881 aaaaaaagtt aaaaataaat caaaaaaaga gagaaataaa ggagcaaggc gcaaataagc
    41941 gcctttttat gctatgatta tgaaaacggg aacaaaattg gttgccagag gaggggttat
    42001 gtgaaagaac gtcaagaaga gatcttgcag ctattgacag aaaaccaaca aggattgaca
    42061 gcaagtgatg tagctgagcg cttaactatc gatcgaagca atgcaagccg atatttaagt
    42121 gaactataca aggcccatca tattgtaaaa accgctggcc gaccagtcgt ttactctttg
    42181 ccaacggaaa agtcaaaatc tgatgaagtg catgtcgact cttctacgca agtgactttt
    42241 gaaacactcg ttggagaaaa tgattcttta aaagtcagca tacagcaggc aaaagctgcg
    42301 attctttatc caccgcgggg gttgcatacg attatttttg gagaaactgg tacagggaag
    42361 tcgatgtttg ctgaatgtat gtatcatttt gcaatcgatt cagagatgct ttctgcagat
    42421 gcgccatttg tttctttcaa ttgtgcggat tatgctcaaa atccgcaact gctctttgga
    42481 cacatctttg ggatcaaaaa aggggcttac acaggagcag ctcaagatag tcccggtcta
    42541 atagccaaag cagacggcgg tatattgttt ttagatgaga tccaccgttt accgccggaa
    42601 gggcaggaaa tgctgtttac tttcattgat aaaggtattt accgtccgct tggagaaagt
    42661 gcacaagtac atgaagcatc tgttcagatc attggggcga cgactgaatc ttcagaaagt
    42721 ttcttatcta cgttcaatcg acggattcct atggcaatca ccttgccgaa tcttgcttca
    42781 cgttcactgg atgagcgtta tgaaatcatc tctcttttta tcaaacaaga agcgaatcgt
    42841 ttgaatcaac gtatcgatat cgaaaaagaa gcgattcttg ccttcatgct ttatgatgcg
    42901 gaaggaaata tcggtcaagt gaaacgagac ttgaaactag tctgtgcaaa atctttttta
    42961 cattaccgta ctcatcatga gaaaaagcta atcattcgaa aagaagaact ttcccttcaa
    43021 gtacaaaaag gattactgaa aatcaaggaa ataccggaac gtctcgaccg attcatggac
    43081 agcaaaagtc agtatttgac atttgaacca ggatttgctg atgttgtctg gtcacaagat
    43141 ccagaacgaa acatgcaggt ctataacgat attgaggaaa aagtcacttc tctttccgaa
    43201 accggtgtgg agaacattga tctagagacc ctcatttcaa aagatgtgga tgcttatttc
    43261 cagacatatg tcaaagaatt gactaaaagt tcgattccca aagatctttt gccagatgaa
    43321 atctggcggt taaccaatca attgtatgat gtggctgaaa aacggttaga ccgtatttat
    43381 aatgaaaaag ctcgttttgc ttttgctttg catctgcaaa gtacattgga tcgtgtgaga
    43441 gaaggtcata tgatcgtcca cccagatttg aataacgtac ggaaaaattt taagagggaa
    43501 ttccaggtag caatcgatct ttcaacgatc attgaagaag aagaacaagt tgagattcct
    43561 tttgatgaaa tcggctttat tagtatgttt ctttctatca atctaggaga aagtgagcct
    43621 gttaaagaaa acaaagtaga tgttctcata ttgatgcacg gcagacagac tgcttcaagc
    43681 atgttggaga tggcacaaga actattagga acaaaagtag ggaaagcttt taatatgcca
    43741 ctggttatgg aagttcaaac aatgtatgaa gaagtgttac aatatgtcaa aaactatcaa
    43801 gagcatttga cgaatggcct actcttgtta acagatatgg gatcattaaa caccttcagt
    43861 gatttgattt atgaagagac tggcattcgg acgaaagcta tcacgatgac aagtaccatg
    43921 attgtgatag aagcgatacg gatggcaagt gttggaagaa gcttagaaga tatttatcaa
    43981 aatatccagc tttcctttga atcgatcgta cgtgatcagt tcaaagcgga aaagtctgaa
    44041 actttacagc gtaaaaaagc cgtaatcgtg acatgcttta ctggtgaagg agtagctgcc
    44101 aaattatatc agcgtattat gccagtgatt aatcaaaata aggtagaact gatccagatg
    44161 cagtttttag aacgtgaatc attcaaaaaa cacatcgatg ccttattgga agaatatgag
    44221 atcaaagcaa ttgcgggaac agttgatgtt gattatcaaa atatcccatt tttttcagcc
    44281 tatgacgttt ttgatgatga aaaactaaat attttaaagc gtatagctga tgatgagatc
    44341 ccgacagcta aaattgtcga ttcacttgct ggatcattaa gcgatgtctt ttctattgat
    44401 gaactgatcc gttcattgca aaagacagtt cagcagatcc agaccgattt gtcgattatc
    44461 gtggaacctg gagtagatac tggaatcgtt atccatttag ccttcatgtt agacggattg
    44521 atcaaacaag aaagccatcg gcaatttgag aaactgtcta ttttctcaaa aatttatcgt
    44581 ttggaaatgg atatcgttcg ggcaaacttg atggcactag aacgaaaata tcaggtaaaa
    44641 attccagaag atgatatcgc atttcttaca caaatgtttc ttgaaaataa aataaaaaaa
    44701 tgaacacact aatcatactg tttacacact aaacatctgt gtgcgcacta taaaaaagag
    44761 ctaaaaagca tgaaaacgct atttcagctc ttttttttat tggcatgctt cttgcgttat
    44821 ataaaagtgt aaaaaaataa aaggagtgtt aaaaatggca aaaaaaacaa tcatgttagt
    44881 ttgttcagca gggatgagta cgagtttatt ggtcacaaaa atgcaaaaag cagcagaaga
    44941 aaaagggatg gaagccgata tttttgcggt ctctgcctca gatgcagata ataatttaga
    45001 atctaagaat gtcgatgtat tattattagg tcctcaagta cgtttcatga aagctcaatt
    45061 tgaacaaaaa cttgcgccta aaggtattcc attagatgtc atcaatatgc aagactatgg
    45121 catgatgaac ggagaaaaag ttcttgcaca agcagaaaat ctaatgaaat aaatcaagta
    45181 tgcagttggg ggtataacaa cgtggaagat caacaaaatc tagaagccat catggggtta
    45241 atcatgtttg gcggtaatgc aaagagcgat gcaatggaag cgattgcggc tgctaaaatc
    45301 ggtgattttg agttagcgga taggaaaatc gctgatgcag aagaatcact tatacaagcg
    45361 catcatgcac aaacagacat gttgacacaa gaagcacaag gaaatcatat gcaagtgaca
    45421 ttattgactg ttcacagtca agatcactta atgacatcta ttgcgtttac tgatttagca
    45481 aaagaaatca tcgaacttta tcgtcgcgta tacagcgaat aaatacaact tcttttttga
    45541 acggaagaag aaaaattttt gaacggattt tttattttgt tataaggaga actctcttta
    45601 tataaaaaac ttataggagg aagtaaaaaa tgaacggact atcaagttgg atggagaaat
    45661 acatcctgcc ggttgccgca aaaatcggag cgcaaaaaca tttagttgct ttgcgtgatg
    45721 cttttatcgg cacactgccc gcaacaatgg cgggatcagt cgctgtaatg atcaatgcaa
    45781 ttattcgtga tttaccgcaa cagtttaaca gtacctatgc tgacagttta gcagcagaca
    45841 aaggaatctt tgccgtattg aatgtcatca tcggaatcaa tggttttgtc tggaacggta
    45901 cactagcaat cgccggattg atctttgcct tttcttgggg gtataatctt gcccgagctt
    45961 atggagtaaa tgatctagct ggggggatcg ttggtttagc taccttgatt caaggtattt
    46021 cctttggtcc agcattatca gcagcattag atgtgaatgt tccttcaaat gtcgcagatg
    46081 caatcaatag ttctgagatt ggtgcaacga ttgcagatgg cacactgtca gttggattat
    46141 ggggttggct aaaactagat catttgaatg gaaacgctta ttttacagtt atgatcatgg
    46201 gagctatttc agtcattatt ttttgtaaat tgatgcttgc caacattacg atcaaaatgc
    46261 ctgattcagt accaccagca gtatctaaag catttgcagc cattttacca gcgacgatcg
    46321 ctttatacgt cattgctatt atcaattata tcgtaggtcg tgtagctgat gggcaactga
    46381 tcattgattt ggtgcaaaaa tatattgcag aaccattcct aggattatct caagggttgg
    46441 gagcagtatt gatcgtcaca gtatttgttc aagtattctg gttctttggt attcatggac
    46501 caaatgtatt ggcaccagtg ctagagggaa tctggggaca agcacaacta atcaatattg
    46561 atattttcca aaaaggttat aacggaaaaa ctggtacacc agccgtgtta gatgcaatcg
    46621 aagatggaaa agcttatatg tgggtccgcg gatcgttcga tgcttttgca tggtttggcg
    46681 gatcaggcgg aacgatcgtc ttactgatag ctatcttgat tttctctaaa cgtgcagatt
    46741 atctaacagt agctaaatta tccattgggc ctggtatctt caatatcaac gaaccagtga
    46801 tgtttggtct gccaatcgta ttgaatgcaa tcatgtttat cccattcttg attgctccag
    46861 tagtagcaac agcaattgga tggacagcta cttatcttgg cctagtcgct ccggtatcgc
    46921 agcaagtcac atgggtagta ccgccgttct tattgtcatt cttagcaaca ggagcggatt
    46981 ggcgggcacc gatcgtaaca ttagtatgta tggtcgtgac tttcttgatt tgggcaccat
    47041 ttgtcatctc tgcaaacaaa atggaccctg ccgcaggcga acaacaataa gatgaacgtg
    47101 aggagcggga cgctttaagt ttcgttcctc ataatgaatt ttcgaggagg aactgaaatg
    47161 attacaattg ccagtgcaat gcaacaagat gaaatcaaag aattgttaga aggatacaat
    47221 gaagaaggaa tgacttttac atttaaagaa aaacaaggaa tcaagctgtt ctttgaaaca
    47281 agtgcagaag atccggaagt tgcggctaaa aaagcaaagg aactaatcaa agcacagcct
    47341 tggggaacag tcctttattt tcaatcaaca gctgtttaga aaatggttta aaaagatagg
    47401 atggttgaga tgataaaaaa cggtttattt ttatgtagta ttggttcttt gatcgtgttg
    47461 tatagtttga atccacattc gcaaaggtat gatttgtata gtatggtcac aggtcttttt
    47521 ctaacaggat taggaatcta tttcttcatg aaggggaaaa aaagagaaga agcaaaggag
    47581 aaaaataact aatggaaatc acgaaaaaat taaatattcc agcatcgttt ttttacgata
    47641 aagttatgga ctctgtcctt ttcgatgtac gtaaagcgac cggcaaatca gtaactagaa
    47701 aacagctgaa aaatttggag tacgtcaaac aattctcgca aaataaccgg gctcgcataa
    47761 aaatcgaaga agtcattgaa aaccagtctt ataagtttcg tacatcgaca acgaagaatg
    47821 aattcgtcgt tcattatcaa atcaaaccat tggacgattc gacctgtgag gttcattaca
    47881 cagaaaaaat ggagtcttat ggcatgcttc aaaagttgaa tgatatgctt ttaggaacaa
    47941 tcttgatgta ttttaaacgg agacgtttca aaaaaatgct agatatgatc gaagcaacgt
    48001 attaaatcaa tgaaacatgg tactatttag ttattgaaaa atgaagtaag ctagaaggaa
    48061 tttttcatag gaagatagat ctttcctgcg aatcctcact agcttttttg taggaagggg
    48121 cagcaaaatg acaaacaaag taagagagct aggtgtatct tcgattgagg aaatgtttga
    48181 tttagccact tatgcattta atggggcaga tacacctgaa aggcgagaac gttttcgtac
    48241 acttgcggag aattcttgga attacggatt ttttgatgag gaggatcggc taacaagtca
    48301 agtcatggcg accccttttc cagtgaattt ttacggacaa gagtacctaa tggccggcat
    48361 tggctatgtt gcttcttatc cagaagcaag gggacaaggc gggatcaatc agatcatgga
    48421 aaagatcctt gaagattgcc gtgatcgtaa agtaagctta tcttatctgg cgccattctc
    48481 ctatcccttc tatcggcgct atggctatga acaatcattt gataaaatca gttatcaact
    48541 aaacagtcgt gatgtgccgt ttatcaaaaa aaaatctgga aaaataaagc gtatggattt
    48601 tgaagatgca aaagctgcaa tcagaagaat ttatgaacag atgccggaaa accaacgagc
    48661 tggtctgaag cgcgaagact ggtggtatac gtataaattc aagcaaaaag gcaattacca
    48721 gtttgctttg cattttgatg ataatgagga agcaacggga tatatcgttt atcagcttgc
    48781 aacgtcttcc tttattattg cggagtgggg atttttggat catgatgctt tttgcagcct
    48841 tgttcgcttc gtaagttctc acaatggtgc ttttgaaaca tttgaatatt catgcggcta
    48901 cggcggtaat gatcaaaatt atctattaga aaatccattt gcttctgtaa tgatcacacc
    48961 ttatatgatg gcgaggatcg tagatatttc tttgtttttg gaaaattatc catttaaaaa
    49021 gagacaagct gcatttgccc tacaaataga agaagatcat tatgcgaaat ggaatgaagg
    49081 aatcgtggaa gtaacaatcg aaaatggaaa aaatcatctc tcaaaagtag aacaaacaaa
    49141 actcccaatc gttcgaggaa gtatccaaca tatcacgcag ctattattag ggtatcgtaa
    49201 agtggaagag ttggtattcc aagaaagaat acaggtgaca aaagaatatg aagaaacgtt
    49261 aagctcaatc ttgccacagc aacagccaat tcttaatgac tatttttgat aaaaaacggg
    49321 tgatgacaat atttttgtca ccaccctttt gtttttcatt tggcatttga aggaaaatgt
    49381 taaagtaaat cctctttttt cgtaaatttg aattttttgt tttcttttgt tttagggttt
    49441 tcttttttgt ggtcttggat tgcttctttc gtaatctttt cccgattttt tttagatgaa
    49501 tctaagcgtt ctgcgtatac tttttctttt ggcttatcat tcattggaaa ggacctccct
    49561 acattatttc attctacctc tagtttatca tctgttaagg actagtggaa acgttttgcc
    49621 taacttataa gctgattttt ttagcagata agcttgcaag taaaaaacaa acaagctaca
    49681 attgatttat tgaaattgtt cctgtagctc agtaggatag agcaatcgcc tcctaagcga
    49741 taggtcgctg gttcaattc
//
LOCUS       DACDIE010000013        49634 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.013, whole genome shotgun sequence.
ACCESSION   DACDIE010000013 DACDIE010000000
VERSION     DACDIE010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.013"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            168..1133
                     /locus_tag="IV250_000854"
     CDS             168..1133
                     /locus_tag="IV250_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAP8768156.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            1510..2553
                     /locus_tag="IV250_000855"
     CDS             1510..2553
                     /locus_tag="IV250_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAP8768157.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(2615..3079)
                     /locus_tag="IV250_000856"
     CDS             complement(2615..3079)
                     /locus_tag="IV250_000856"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAP8768158.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            3325..4272
                     /locus_tag="IV250_000857"
     CDS             3325..4272
                     /locus_tag="IV250_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP8768159.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            complement(4483..6555)
                     /locus_tag="IV250_000858"
     CDS             complement(4483..6555)
                     /locus_tag="IV250_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP8768160.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(6488..6865)
                     /locus_tag="IV250_000859"
     CDS             complement(6488..6865)
                     /locus_tag="IV250_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAP8768161.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(6994..7554)
                     /locus_tag="IV250_000860"
     CDS             complement(6994..7554)
                     /locus_tag="IV250_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8768162.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            7704..8996
                     /locus_tag="IV250_000861"
     CDS             7704..8996
                     /locus_tag="IV250_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP8768163.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            complement(9056..10174)
                     /locus_tag="IV250_000862"
     CDS             complement(9056..10174)
                     /locus_tag="IV250_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAP8768164.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(10188..11609)
                     /gene="arcD"
                     /locus_tag="IV250_000863"
     CDS             complement(10188..11609)
                     /gene="arcD"
                     /locus_tag="IV250_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HAP8768165.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(11855..12976)
                     /gene="mnmA"
                     /locus_tag="IV250_000864"
     CDS             complement(11855..12976)
                     /gene="mnmA"
                     /locus_tag="IV250_000864"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAP8768166.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            13380..13979
                     /locus_tag="IV250_000865"
     CDS             13380..13979
                     /locus_tag="IV250_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAP8768167.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(14117..15403)
                     /gene="efmA"
                     /locus_tag="IV250_000866"
     CDS             complement(14117..15403)
                     /gene="efmA"
                     /locus_tag="IV250_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HAP8768168.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(15674..16018)
                     /locus_tag="IV250_000867"
     CDS             complement(15674..16018)
                     /locus_tag="IV250_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HAP8768169.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(16155..17291)
                     /locus_tag="IV250_000868"
     CDS             complement(16155..17291)
                     /locus_tag="IV250_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP8768170.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            complement(17480..18454)
                     /locus_tag="IV250_000869"
     CDS             complement(17480..18454)
                     /locus_tag="IV250_000869"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP8768171.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(18534..19409)
                     /locus_tag="IV250_000870"
     CDS             complement(18534..19409)
                     /locus_tag="IV250_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HAP8768172.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            19539..21446
                     /locus_tag="IV250_000871"
     CDS             19539..21446
                     /locus_tag="IV250_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAP8768173.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(21987..25265)
                     /locus_tag="IV250_000872"
     CDS             complement(21987..25265)
                     /locus_tag="IV250_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HAP8768174.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(25536..26081)
                     /locus_tag="IV250_000873"
     CDS             complement(25536..26081)
                     /locus_tag="IV250_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP8768175.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(26093..26785)
                     /locus_tag="IV250_000874"
     CDS             complement(26093..26785)
                     /locus_tag="IV250_000874"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAP8768176.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(26804..27088)
                     /locus_tag="IV250_000875"
     CDS             complement(26804..27088)
                     /locus_tag="IV250_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8768177.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(27101..27664)
                     /locus_tag="IV250_000876"
     CDS             complement(27101..27664)
                     /locus_tag="IV250_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP8768178.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            27892..28554
                     /locus_tag="IV250_000877"
     CDS             27892..28554
                     /locus_tag="IV250_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HAP8768179.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            28678..29874
                     /locus_tag="IV250_000878"
     CDS             28678..29874
                     /locus_tag="IV250_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAP8768180.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(29975..31351)
                     /locus_tag="IV250_000879"
     CDS             complement(29975..31351)
                     /locus_tag="IV250_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8768181.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(31388..32254)
                     /locus_tag="IV250_000880"
     CDS             complement(31388..32254)
                     /locus_tag="IV250_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HAP8768182.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(32343..33368)
                     /locus_tag="IV250_000881"
     CDS             complement(32343..33368)
                     /locus_tag="IV250_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8768183.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(33386..34060)
                     /locus_tag="IV250_000882"
     CDS             complement(33386..34060)
                     /locus_tag="IV250_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8768184.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(34244..35137)
                     /locus_tag="IV250_000883"
     CDS             complement(34244..35137)
                     /locus_tag="IV250_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8768185.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(35211..36908)
                     /locus_tag="IV250_000884"
     CDS             complement(35211..36908)
                     /locus_tag="IV250_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP8768186.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(36901..38214)
                     /locus_tag="IV250_000885"
     CDS             complement(36901..38214)
                     /locus_tag="IV250_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP8768187.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(38214..41732)
                     /locus_tag="IV250_000886"
     CDS             complement(38214..41732)
                     /locus_tag="IV250_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAP8768188.1"
                     /translation="MDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEK
                     KEISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMV
                     NFYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETL
                     YKDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDV
                     LFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGS
                     SNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAI
                     GRNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVS
                     PFGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYK
                     FSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSS
                     SIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFET
                     KMPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEY
                     TSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNI
                     NKLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDG
                     KYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRL
                     YDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTL
                     DGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQP
                     LHLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIID
                     GVLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPND
                     SSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTL
                     KVEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYIL
                     LAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(42025..42483)
                     /locus_tag="IV250_000887"
     CDS             complement(42025..42483)
                     /locus_tag="IV250_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP8768189.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(42780..43298)
                     /locus_tag="IV250_000888"
     CDS             complement(42780..43298)
                     /locus_tag="IV250_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAP8768190.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(43300..43917)
                     /locus_tag="IV250_000889"
     CDS             complement(43300..43917)
                     /locus_tag="IV250_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAP8768191.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(43914..44768)
                     /locus_tag="IV250_000890"
     CDS             complement(43914..44768)
                     /locus_tag="IV250_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8768192.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(44765..45676)
                     /locus_tag="IV250_000891"
     CDS             complement(44765..45676)
                     /locus_tag="IV250_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8768193.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(45717..47051)
                     /locus_tag="IV250_000892"
     CDS             complement(45717..47051)
                     /locus_tag="IV250_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8768194.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            47259..48089
                     /locus_tag="IV250_000893"
     CDS             47259..48089
                     /locus_tag="IV250_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HAP8768195.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(48333..48582)
                     /locus_tag="IV250_000894"
                     /pseudo
     CDS             complement(48333..48582)
                     /locus_tag="IV250_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(48773..48967)
                     /locus_tag="IV250_000895"
     CDS             complement(48773..48967)
                     /locus_tag="IV250_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8768196.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
BASE COUNT        14311 a        10574 c         8806 g        15943 t
ORIGIN      
        1 ccatctataa tagaaatgta caacaaaaca gttgctgatc caactagtaa cttagatgaa
       61 gacaatcagc tgtctgcttg ctcgtaatca tacattccta ttcattttac aatgtattcg
      121 ttcatcttgg tactaaaatc tattaaataa aaaggagaat attatatatg aagaactttt
      181 tgaaaattat tccgatcccg atctgcgggt tgatccttgg gttagcttcc cttggaaatc
      241 tgtttaaaga ctatcaccat gttgctttag gcaatctaac tggcggtatc tctatgattc
      301 ttatgatatt catttttgtt aaattgttta tcttattcga acacacgaaa caaacgttgc
      361 atgatccaat catcgcctcc gtttcaccaa cttttacgat gtcactcatg gttatttgta
      421 cttattttgt ttcatatgaa agtattgcac caattgtcaa attcatttgg ctgtgcgctg
      481 ttatcttcca agtgatcctt gtcttttatt tcaattatca ccatgttatc aaagctgatc
      541 tttcaattga agcaatttat ccaagctggt ttatcattta cgttggattt ggggtcatca
      601 ctgtaactgc cgggaacttc ttcccactta ttggaaaaat tttcttttgg atttcattga
      661 tctgctatgc gttcttattg ccgatcatca tcaaacgtgt ctttttcgta agaaatatgg
      721 ctcatccgac acgtccgctg attacgatcg ttgcagcacc tggttctctt tgcctaacag
      781 gttatcttaa aaactttgct gaacctaatt tttatcttgt cgtagcattg ttcgctttat
      841 ctcaagtatt gtatttcgtt gtgctgtcta tgcttccacg tttgttcaag cttcgttttt
      901 atccaagcta tgcagccttt acattcccgc ttgttatctc tgcaacagct atatttacta
      961 ctgttcatta cttcaacagt ttaggtatct atagtagcgt attggatttc ttaaaagttg
     1021 cggaatgtct gtttgcaagt gtagttgtct tctacgttct tgctcattac gtacgtttct
     1081 taattaaaga acatagcaaa catggaaaat ttgtttctaa aaaaatcgct taataatcga
     1141 ttcaataggt tgtgaaaaag ctgttctgca tcaagtaata agaacggcca aacaaaaaca
     1201 gctgttcatg tttttgtact ggttgggctt attaccgcag atgcaagctt ttgaacaccg
     1261 tttaccaaga ggtcgtaaaa gaagtggtca gcttcaagaa ataagaagga atttacgaaa
     1321 attgcttctc aaatttttgt gaaatttcgg cttatttccg tagaagctac ttctggaaca
     1381 ccgtttattc gaaacaagag gtcgtacaag acttttgtca cgacctcttg ttcttttgtc
     1441 aaaatactgg tttagtgcta ctatgttcga gtatgctttt agtatttatt gacgaaagga
     1501 accgatcaaa tgaaatcatt gtcacatcca ataaaatctg taaaggattc agcggtccgc
     1561 gaaatcttac ccggtttact gctttctttt gccatcgctg ccttcagcag gcttcttgct
     1621 atttatctgc cacagttagg tggagcgact attgctattt tattgggtat tttattagga
     1681 aatacttttt ttaaacagcc tgttctagca atcggcacaa aatttgctga aagcaaacta
     1741 ttagaattct ctgttttttt actaggtgca actgtcacag ctcaaacgat tgctcaaatt
     1801 ggtgtaaaag gaattgtttt tgtgttactg caaatgtcag tcactatcat cggtgcttac
     1861 ttgattggta aaaaactgca attcagcgat acttcttctt tgatgatggc tggaggaaac
     1921 gctgtttgtg gttcttctgc tattgcttca atcgcccctt ccatccatgc gaaagaggaa
     1981 gaaaaaggac agatcatcac actagtcaat ctactgggta ctgtgatgat gttgactctc
     2041 ccttttcttg gattacagtt gtttggtgga aatctcattg aaaaaagtgc acttttgggt
     2101 ggtacactcc aatctgttgg tcaagtagtc gctggggcaa gcttacttga caattctgtt
     2161 gtcgaattcg ccatgctatt taagattcta cgtattatga tgctcgttgt tgttgtctat
     2221 ctgtttgaaa aattcatgca caagaaacaa ccagctctgt ttgaaacgcc aactgatacg
     2281 cagaaaaaac gaaaattacc gcttccttgg tatgtattag ggtttgtgat cttttgtttg
     2341 gtcaacagct tgttttcttt acctgtagtt attgaccaat cggcacattt tatcagcagc
     2401 tggtttgaga tcactgcttt agcggcaatt ggtctgcgtt tagactttgc aaaattcatg
     2461 aaagaaggta cacgcttctt atcttatgga ttagctgtcg ggcttctcca aacagtgatg
     2521 gctttactat ttatttggct gatcaaagtt tagaatgaac atgaaaaaac agttgtgatt
     2581 ctcgcatgga acaatgtccg aatcacaact gtttttaatt ttgcagatat ccttctaaca
     2641 acaacaattg gcgtttgatt tcaaccccat ctattgcgtt tcccatatat cctgtcagtt
     2701 ttccattttg cccgatcact cgatgacaag gaataacgat tggcaatgga ttggttcgat
     2761 ttgcctgccc aatcgctcgc acagctttcg gactatcaac agcaacggct acttctttat
     2821 acgttcggat ttctccataa ggaatggtcg acaaagcttt ccagacattt tgctgaaaag
     2881 gcgtacctct ctttatagat aaaggaacat caaattcttt tctttttccc gcaaaatatt
     2941 cttcaaactg ttcttgtgtg cgcgttaaaa ttcgctgatt cgctgattcg ctttctgaaa
     3001 ttggctgaaa agatacagca gtcaatccgg attcatctgc atctaaccat aaaaagccga
     3061 taggtgtgtg tattttcatt ttgtttgctc ctagtcaata gactggaact tcctcctttt
     3121 tctctatcca aaactacttg ccaactaata aaaattgttc ttaaaagttt gataaaccga
     3181 ggaaacatgg taaactaaat acgttagttt acaaaaagaa tacctgttct tttacgtaaa
     3241 atcaaatttt aaaggtgtta tttaaggttt attaaatttt gaatgtaaac catatataaa
     3301 catacgattg aaaggataga aacgatgaaa gtatcaattg ttgtcccatg ttttaatgaa
     3361 gaaaaaactg ttcctctttt ttttgatgag gttgaaaaat tcaaaggtgc ccatgagttc
     3421 gaatatttgt ttattgacga cggatcaaaa gacggtacgt tagcagcaat caagaagcta
     3481 gcctcagctt atccttatgt ccgctttgtc tcattttcca gaaattttgg gaaagaagcg
     3541 gctctgtacg ctggattgca agcttctaca ggagatttag tgaccgtgat ggatgttgat
     3601 ctgcaagatc cgcccgaact gcttcctgag atgatcaacc ggatcactac ttctgatatc
     3661 gattgtgtcg gcacccggcg ttcgactcgt gaaggcgagc ctgcgatacg cagtttcttt
     3721 gcgaaaaaat tctaccagtt gatcaataaa atcagcgata cagaaatggt cgatggcgct
     3781 cgtgatttcc gcttgatgac tcgtcaaatg gtggatgccg tcctagaatt aacagaatac
     3841 aaccgatttt caaaaggatt atttagctgg gtcggattta aaacagaata tatttcttat
     3901 gaaaaccgcg aacgtgtggc tggcgaaaca tcttggtcct tctggaaatt attcaactac
     3961 tcgattgatg gtatcgtgaa cttctcggat gcaccgttaa cgattgcttc atttatcggc
     4021 gctttgtctt gtgtcggttc tggtattgca attttgttca ttattttgcg ggcattgttg
     4081 tttggtgatc ctacatcagg atggccttcc atggtctcga tcttcctgtt cattggtgga
     4141 atccaattgt tatgtcttgg aatcatcggg aaatatattg ggaaaatctt cttagaaacg
     4201 aaacagcgtc ctgtttatat cataagagaa acagaaaaag tgaagccaaa tgaattgaag
     4261 cctcaccaat aatatacgtc aaatgaagta aacatttttt taaatgaaaa aggactgtga
     4321 caaaagtctt gtcacagtcc ttttttccga ataaatggtg ttccagaagt agcttcttcg
     4381 gaaataagcc gaaatttcac aaaaatttga gaagcaattt tcgtaaattc cttcttattt
     4441 cttgaagcag accacttcta tcacaacctc tttcatatct tattacttct tcattgctgc
     4501 ttttagtgct gctgctaatg ggctttcttc tggttcttct gcttgtgcat attttttcag
     4561 caagcgtcgt tcttcatgtt tggtcatttt cttcttctgc tcttttttat caagcaattt
     4621 ttctgtcgtt ccatcgtatt tacaacggaa ataagcaccg tttttacctt cgatgatttc
     4681 cattttccgg tgacattgcg ggcatcgatg attagagact tttggatctt ttcttctgcg
     4741 gtagttgcaa tctgaattgg tgcagacata gattttgccg tcacgtgtgt ttttctcgcg
     4801 cagattggcc ccacattctg ggcatttctt ttgggtaatc gagaaatctt gatattcttg
     4861 tttgcttgat ttgatctcat tcaccaagcg ttttgtttct ttttcgattc cattcaagaa
     4921 ggttcctgct tgtttctttc ctgcagcaat agcttcaagg tctttttccc agctttctgt
     4981 caaatctggt gttactaaag agggatttac caatactaat aattgcttac ctttaggaga
     5041 tacttgcagt ccttgagggg tacgttccat cagttcagat ttgattagtt tctcgataat
     5101 ctctgcacgc gtagctgggg ttcccagact gtatttttcc atttgaccaa gcaagcttgc
     5161 ttctgacaat ggtttaggcg gtgcagatag ttcttttttg atggcgaaat cgggattgat
     5221 tctcattcct ttttcccatt tgactgtttc cgtctcagtt tcagtttcgc ctttccatcc
     5281 tgcttggacg acacgatttt gccgaaaaat gaatcgttct ttaccaaatt caaccgtaac
     5341 ttttgtttgg ctgactttat ggggttgagc aaacagtcct aaaaaacgag agacaatcat
     5401 ttgatagatt tttcgctctt cattacttaa tttttcataa cgtgctctgt tttcagttgg
     5461 caaaagtgca tggtgatcgg tcactttact gtcttggaat acttttgtct gttggacgat
     5521 ggctccgttt ttcaaatact gcttgacctc aggagcaaag tcgctgactg cttgcaatcg
     5581 ttctttcatc gtacttttca tgtcatttgt cagatatttg ctgtctgtac gcggataagt
     5641 aacgatctta tgggtctcat acaaactttg tacaagactt aatgttttct ttgcagaata
     5701 gccatagcgt tgattggctt ctctttggat ctctgttaaa tcataaggca atggcgcaga
     5761 ttccgttttt aatttttctt ggacttctgt gacagttcct ttgcttgtac tgatcaattg
     5821 aacaagtttt tgtgcttctt cttgtgtttt taaagcaaat tgattttttt gaagcatttt
     5881 ggcttgttct tttccagctt gcaacgtgat cgtgaaatac gtttgtggac ggaactgctc
     5941 gattttcttt tcttggtctc tcactaatgc tagtgtcggt gtctgcacac ggcctgctga
     6001 taaattatcc gcgtacttca cagttaaggc tctggtcaca ttcaatccta ccagccagtc
     6061 cgcttttgcc cgggcaagag cagattcata caatgcatca tattctttcg caggtcttag
     6121 ttgcttgaat ccagttttga ttgctttatc tgtttgagaa gagatccata accgcttcac
     6181 tggtttattg aaatgcacat actctaagat ccagcgagca acaagttccc cttctcttcc
     6241 ggcatctgtc gcaatgactg cttctgagac atcttttcgc tgagccagct gcttgattgc
     6301 ttttaattga tgtcctgttt taggaagtgg tttgattcct agttttttag ggatcattgg
     6361 caacgtgtcc atttgccagc tctgccattc cttattcaga tcttccggca ttttcaatcc
     6421 taataaatga ccaagggccc aagtaacgat caccttgggt ccttcataat aatttttgtg
     6481 cttttgatta gcaccaagca ccttgcttag gtctttcgca acgcttggtt tttccgctag
     6541 aattagttgt ttcataaaat tcctttctgc tttctactgt gatgggctga tgatttttca
     6601 acaacgcaag tcccttgtat tgctgtaaat catcgtcaca ctcttcacac cagcgctcat
     6661 ctccgtcatt ccaaaaggcg ctgagaaagt tactttttgc attgatatat tcataatttt
     6721 gatggatttc gttccattgt ttttcgtacg cttgtcgtgc ttcttcgaaa gactcgaatg
     6781 tttcttcttc ttcaatatct tcttcccagt cttcaaaaaa ccaccaaggc tcattgtccc
     6841 cgaacatggt aattacttga tacatactcg tgctccttct ttcagctttt ctagactcat
     6901 cttagtcact tttcaagaaa gttgcaagaa ttttctctta taaaagggtc atttaatgaa
     6961 catcttaaaa ttgttacaaa tgaatctgat tgtttaaggc aaagtagcga taaagttatt
     7021 gatcatccaa aaatagctgt cttctggatc gattgttggt tcttttgtaa agtatcctaa
     7081 aaaacgtaag gaaagataac cgtggatcat actacgaagt tccctgcttc gatgaacttt
     7141 ttcttctttc gttaattgaa aagcctcaaa tatctgcaaa atgatctgat tcgtttcatg
     7201 gattccatct aataaaacag cttcattggt atgcggtaca ctgatcagta actcatacac
     7261 tgcttgattt gaaaaagcaa aggatcgata agtttctgca tagatctgca gtgcttcctg
     7321 accacttttt cccacaagag cccgtcgcag ctggtcatta agttcctgca gaagtttcgc
     7381 tgttagcgct gttttgacat cacgaatatt tttgaaatgg ttatacagcg agggagcctt
     7441 gatatccaag cgtttggcta aatgctgaaa ggttatttgc tgaacatcca tctcttctgc
     7501 tagttctcga aagcagtcga tgattttctc ttgagacaga tttctttttt tcatttccat
     7561 gtttcctctc ataaaataaa tccttcactc tttcagttta ccacgaaaaa gaaagagaaa
     7621 aaagagactt tcattgataa actacatatt gtagtttata ataaagagct gtagatgaaa
     7681 attttcagaa agaaggagtc taattggctg caagaataaa ggaaaaatta tttaggaaaa
     7741 gagaaacttg ggaacaaaac ttgtttgtct tatggtttgg aacatttatg gctggtatcg
     7801 gatttagttt agtcatgcct tttatgtcac tttacataga tacgttaggt aattatagta
     7861 cgtcacaatt gaacttttgg agcggattga ctttttcatc tactttcttg gtgactacgc
     7921 ttatctctcc ttggtgggga aaattagccg accaaaaagg aagaaaattg atgctgttgc
     7981 gtgcttcatt aggaatggca atcgtcattt ctttgatggg ttgcgtgact agtgtgtatc
     8041 aattagtcgc attgcgtctg cttcaaggga ttttttctgg ttatatcagt aatgccactg
     8101 cccttgtcgc aactgggacg ccaaaagaaa aaagtggtca agttcttgga accttagcaa
     8161 ctggttctgt tacaggaaca ttacttggac cgcttcttgg cgggatctct gcttcagcgt
     8221 ttggctatcg ccctaccttt ttcattacag ggatcatctt atttttcgtc ttcttgttga
     8281 gtctgttttt cgtccatgaa aaatttgtcc cagtgaaaaa agaaaacatg gtttctgcca
     8341 aacagatttt caaagacttg aaatatccac acgtcgtcat cggtatgttc gtcacaacga
     8401 tgatcatcca agcatccaac aattccatta gtccaatcat cagtctgtac attcgccaat
     8461 tgctgcatgg acatgggaat gttacgttga tcagcggaat cattgcttcg atacctggga
     8521 ttgccacttt gatagccgct ccaagatttg gtcgcttagg tgacaagatt gggagtgaaa
     8581 gaatacttgc gattggtttg ggttttgcga ttctggttta tatcccaatg gcattcgtca
     8641 cgaatgtttg gcaattagct gccttacgtt ttttgattgg tatctctgat gcttgtctgc
     8701 tccctgctgt ccaagcattg atcacaaaat attcgcctca tcaagctgct ggacggatct
     8761 tcagttataa ccaatctttc caagcaactg gtaacgtagt agggcctatg atcggctcca
     8821 gtgtttccag tgcatttggt tatcgcggcg tctttctttc gacttctctt cttgttttgg
     8881 tcaacttctt gcttgtacga cacaatacac aagagatcca tcagcaagaa actgcagaag
     8941 aaaaagcttc aggagtctcc atagcttcag ggcactctcc ttctcataga caataagcgt
     9001 aaatgaaaaa cactgcttga acagttgtca cagactgtcc ttgcagtgtt tttgtttagg
     9061 cctgtttttt caagaactca gataagagga ataatccctt ttccaaggtt tcttttttac
     9121 aacagtaacc tagtcgggca tgcttcggta aatcaaaggc acttcctggt actaatagta
     9181 ctcctgtttc ttctaataaa ttgatacaaa atgtctggtc atcttcttcg atggctaatt
     9241 taatgaaaga agtagagacg taattgggag cgacaagttc aactttcggt tcattggcta
     9301 cccattgttt taaaatcaca aggttctctg taatgatctt tttgtttcgc gccaatattt
     9361 tttctttatt cttcaatgca tgtacagcaa gatcatctga caggactccg ccgcaaatca
     9421 tcgtgtagtc tcgatacttt cgaaaaactt ctgccaactc aggtccagta gctgtccagc
     9481 caatacgaat acctggaatg gagtaggttt tggataggga attggttgct ataccttttt
     9541 catagacatc tacgatcgac aagaattcac ctttatccgt taaaggcgca tatacttcat
     9601 ctatcaatac atatgcatct actgtacgag caatttctgc gattttttct aaagttttcc
     9661 gatctagcaa tgtgcctgta ggattgtttg cactattcaa acagatgatt tttgtttcag
     9721 gtttgactaa ggcttctagc tgttctaaat caaactgcca atcttcttct tccttcaaat
     9781 gaacaaagtc aacagcggca cctaaagatt tggggatgtc atataattgc tgataagtag
     9841 gaagcatcga aacaacatgg tctcctgctt ccactaacgc gtatagagct aaaagattag
     9901 caccagtcgc tccattcgtt tgtaagatgt tttccggatc gacggtttga taaagagaag
     9961 cgacttcttg tttgaaaaca tctgatcctt cgatccagcc atagtccaat ggttcgtttt
    10021 tgcgtttttc gaaaaattta cttacgctag tcccatctaa gccaacgagt tcgtctaatg
    10081 tcaatgcttc gatcgagctt tgtgcaatat catataccgc tttcttttcc catttattta
    10141 gccattcttc tacaccgaaa tttgcgattt tcatgtttct ttccccctta tatcttgatc
    10201 cagcctacag caagcaaccc gattgaaagc agtgcggcca cacaaatcag cccaatcatt
    10261 actttttctt ttcccgtaat ttgatgtttg gtttcacgac aagcgatcca gtagaagcaa
    10321 aaaccaggaa gataggcaat actacataaa aggacataag aaaatcctgc taaaatgatg
    10381 cccatcaatt caaaaactac agcaatccca ccgatcacta cttgtttttt gattccttct
    10441 tttaaagaaa actggacttg atagatcccg acaagtagat aacaaatcaa aattgccgct
    10501 gtacataaag aataggcaaa ttgataggca taatcagtaa ctaagaaagt caataagaat
    10561 agattagtca agattgcagt aacaactaac gaataagttg gggcattttt tttattcgtt
    10621 tttccccaac ctttcggcaa cagaccgtct cgagccatca acatcgttgt ttctgctggg
    10681 agcatcgtcc aagacaacca agaaccaatg atcgaaataa tcaagccgat attgatcagc
    10741 gctgctcccc aatttcccac gattcctttt aaaatatttg ccatagcagg ttgtgaaata
    10801 gttgctaact cttcttgaga taagacgccg tatggcaaga tcgaagcaag gatatagata
    10861 aacaataaac aaagcagacc aatgatcgat gcttgctggg cttctgaacg tttcttcgcg
    10921 cggttggcta aaacacttgc gccttcgatc cctacaaaga cccacatcat aaccatcaaa
    10981 caacctttaa tctgcaacca gatcgttcct tgctgccctg ttccattcac tagattatca
    11041 gatacgttac cccaaaaatc agcagtgaat atattcactt taaatgcgat aaatacaaat
    11101 attaaaaata ggaataatgg cactaattta cagatcgtca cgattgtatt gatgacgctc
    11161 gctccttcaa ctccattgtt tactaataaa gtcagacccc aaataaaaat acttgcaaat
    11221 aaaatagaag gaacattttg tccacctttg aatatcggga aaaattctcc catggcgctc
    11281 atcagcatcg ttgcaaaagc aacatttccc agccacgcag acaaccagta accccaacca
    11341 ctgatgaagc cacctaattt accaaatgaa gcttccgcgt aaccgaaaat accaccatct
    11401 agatctggac gcttttctcc taaatgattc agcgataata ccaaagctaa aataccaatc
    11461 ccaacgatta tccatgaaag gatcgctggc cctggagctg cagaactagc cagatcactt
    11521 gtgatcccaa acacaccgct tccgattgac gaactgacaa ccagagcaac cagtccccat
    11581 ttgctaatac ccgtcgattt cttttccatt tccatcaccc tcacttataa aattgaacaa
    11641 atcacttgca aaagattccc ttctattttg caactgctct tgcttaacaa aaagcaactt
    11701 tattcatatt attggatatt atgaactttt tacacaagta tttcaagtat aaaatgaata
    11761 aaaattaatt ttattgcaaa aaaacagcta tgctatccaa aagaaaaggt gtttcttttg
    11821 ttagccatag ctgttttgat tcatttaata tcgcttaaac gtattgtaaa acttttgttt
    11881 cttgatatgc gtggtcgatc aaaccaccac ccagacattc cattccatca taaaatacaa
    11941 ctgcttgtcc aggagtgatt gcacgtacag gttcgtcgaa tacgacttct gcacgattat
    12001 catccaataa gcgaactgtc acaggaacgt cttgctgacg ataacggaat ttcgctgtac
    12061 acttgaattc ttttggcatt ggttcattcg tcgtaaagtg gatttcactt gcatccaagc
    12121 ttgtggcata caatgcagga tgatggaacc cttgccccac atataatgtg tttgtcgcta
    12181 gatcttttcc tacaacaaac cagggttctt gcgaatctcc tccgccaccg atccctagtc
    12241 cttgacgttg accaatcgta taatacatca agccagcatg ttgtcctttg acttcaccat
    12301 caagagtgac catatttcct ttttttgctg gcagatagtt gctcaaaaat tgtttgaagt
    12361 tcttttcacc aataaagcag attcccgttg aatctttttt cttagcagtc gcaaggcctg
    12421 cgcgttcagc aattgcccgt acttctgatt tttccatgcc gcctagaggg aacatcgttt
    12481 ttgaaagctg ttcttgtgat aattggctaa ggaagtaagt ttgatcttta ttattgtcta
    12541 ctccacgaag catgtggacc acgccgtttt catcgcgagt gacttgcgca taatgtccag
    12601 tagctacata atcggcgcca agatccattg cataatccaa gaatgctttg aatttgattt
    12661 ctttgttgca catcacgtct gggttaggtg tacgtcctgc gcggtattcg gctaggaaat
    12721 attcaaatac acgatcccaa tattcttttt caaaattcac agagtaataa ggaatcccga
    12781 tttgagcagc tactttcgct acatccttat aatcttccgt ggccgtgcat acaccgtttt
    12841 cgtccgtgtc atcccaattt ttcatgaata ttccgatcac gtcgtatcct tgttccttta
    12901 acaaaagtgc tgttacagat gagtcgacac cgccgctcat ccctacgaca acgcgtgttt
    12961 tgctgttgtc tgtcatgata tcaccatcat ttcaattaga ttctgaaaca tctacgagtt
    13021 gaaggtcgca acttttcagt attttacatt atacgtcata tttctgaaaa tttcatccct
    13081 taattatcaa gtcgtaaaaa tactgttcta ctggacctat tcagtacaca aacatgatag
    13141 tcaaccaaaa aatcactcat cttttgagca ttcgatataa aaaattggca tactcataaa
    13201 aagggccagc ctttcggcaa taaggaataa ttttataaat tgtgaaaaac tatttttcaa
    13261 aattgcttct tatcgcttaa agctgcacgt ctcatttaca accttttctt tttagatgtt
    13321 atgagttgct cagataagtg attttgttta ctctaagaaa aaagggaggc aaaagataca
    13381 tgaattactt tatcagtgat atgcactttt tccatgaacg cttattagga aaaaacgact
    13441 ttgctccgcg gctctttgca aatgtggatg aaatgaacca acagttgatc actagctgga
    13501 atgcagtaat aaaagaaacc gaccatgttt atcatctagg cgaccttgct atgcatccgc
    13561 aatatgaaaa agcaaatgca gaaattttgg aactggtcac tcaactgcaa ggaacaatcc
    13621 acttgatcaa agggaaccat gacagtcgcg cttttttccg ttatttagaa aaaaatgatc
    13681 ctaggcttac tgacggaagt cccaaatttt atttttatga tgtaggtgcc atcgttaaat
    13741 tcgaccatca acaatattac ttgactcatt atccgatgtt gctcgggcct aacgacacta
    13801 tccgcaatct tcatggacat atccatcatt acagcgtacc tatcgagaac gatgtcaatg
    13861 taggcgtaga cgcaccggaa agagaactct taaaggaaaa actaccattt ggcactccgt
    13921 tatctgagca acaaatcaaa gaaatctatg aagcaaagag gaagtacgat aggtcgtgaa
    13981 aaaactgccc tatatcaagt attaaacaaa taaagataat tactcatatt tttcatttga
    14041 gtaggcttat tgccacagga acattaggat cagaaatgga gttgtaacaa gacttctgtc
    14101 acaactccat tccatactat tcgtcttttt gctcatggtg ttcttttacc agttttttct
    14161 gtaattcttt atcataattt cgggcagctg acgttaaaaa taaaacgatt ccgcataata
    14221 aaatcccgat tccggaaaac aagaacattt tttctacccc taaacgatca gctaatggtc
    14281 ctgcaaataa taatccaatt ggacctgcca ggttcatcaa agagttgaag ttccctaaga
    14341 cacgacctaa aacattcgat tcataactct gctggatcat tgccatcagc agtgtattga
    14401 aatatggcgt tgcacaacct gctcctgcat tcaagataag aaagtacaag aacccttgac
    14461 tatcgtttgg aaccaatccg cttgcaccga tcgtcaggcc cataatgatg taggctataa
    14521 taataggttt cattcggtct ttccatgttc ctataaaact gatcagtgca ccgcctaaga
    14581 gcattccgac tgcgtagact acttccactg ctcccgcctg tccaacggaa ccaccaaagt
    14641 aatctagcgt catcaatgga tacagactga ttgctggcat gaacaacagc atcacaaatg
    14701 caccattgat cgttatgtac cataaccctt tattatccat caattgctgc agcccaaatt
    14761 ttgcatcttt aaaaatagta agcggaactt ccaatatttc aggagaaact tttgggatct
    14821 tcacaaaaat caataagccg acaccaaaaa ctgcccccaa tacatctaat aaaataaggt
    14881 aattgacagg aacgactgaa aacaaagccg cacctagagc tggcgcaatg atgaaattcg
    14941 ctgattggac cattcctaac tgtccattcg tccttgttat gtgagaagat ggaacgattg
    15001 tcggcataat cgactggatc gttggcattt ggaatgtttg tgcgaccgaa cgcataaaca
    15061 aagacacaaa aactagccaa atcgggaagg attctgaaat cgttccgact actgctaaaa
    15121 taagcgcgaa aatcgctaca ataatatccg tgacaatcaa aagagctttt ttatcccagc
    15181 gatccactag cggtcctaca aatgggctta gtaaaaccat gggaatcatt cctagtaaag
    15241 tagcaaaact caaaattgtc gccgaaccgg tttctcttgt caaataccag atgatcgcat
    15301 attggacgac catactagtg attcctgaca aaaactgtcc cgataaaaag aacaaataat
    15361 ttctcttcca attcgttaaa acgaccgact gttcattttc cataaacatc cctcttttct
    15421 ttttcatttt gtatagcgtt tgcacaacaa tcattatacg ggattctttt ttcaaaaaat
    15481 acctttttcc ctaaataaca atcgtttcaa tttaaatttc tttacttaga aacaataaat
    15541 cagcaaaata ttaaaaataa caacatgtgt taaatttaaa aataaaaaag acaactaatt
    15601 tgcgtcgtcc ttacaaagtt agtcatctaa caataggccg tttcaaaact agttgtcttt
    15661 ttatatccac agatcattcg actttttcga ataacccacg gctaatcatc ccatccatca
    15721 gaaaataaaa tttatcttgg ctcattttct gttttggatc tttgaaaata ttgactgctt
    15781 tcaatccatt tgctgtagtg atcgacattt tcaatgtttg aatatctttt gatacagtga
    15841 tttttaattt gaatccttct ttgctttgcg gcgtggcatc taatggtcgg atcaattgat
    15901 aattgcctcc gtttgtttct gtaaatccat tatcgcgcaa tgtatatctt tttgcatcag
    15961 gatgcaaacg atagctaatt ggtgcgccca gtaccgttgc tgttgtttca aatgccatga
    16021 ttttcacctc gataaaatta gtttctcttt attataaaag tttatcctga tttttactag
    16081 gtttttcagt taagttccct aaaaaatgaa ggcgttctct cagacatctg ttcatataag
    16141 aatgacttca ctagttattt tttgacagca cgaactaatt gatcaaggaa atactggatg
    16201 tcgtcttcgg tatttcccaa accaaagctg atacggatgg attcttttag tatcggtgta
    16261 tcttctccat acattgcttc taatacgtgt gaaggctcga tatttcctgc tgtacaagct
    16321 gaaccagtag aaatagcgac cccattaaga tcaagcttca tcagtagcaa atcatttgct
    16381 tcgccaatta agcgaagatt caacacatga gggagtttat tcgttaaatc accatttact
    16441 tgataaggga tttcgtttgc ttctaatgta tcaagaatca attgctggaa tcctttatat
    16501 ttgttgttac gtgcttcacg ttcttcaggt gacagcagtt caactgcttt tgccataccc
    16561 cagatccctg ctaaattttc tgtacctgca cgccgctttt cttcctgctc cccgcctaaa
    16621 agcaatgcag ggatatttac tccgtctcgt ttatataaga aaccgacgcc ttttggccca
    16681 ttgattttat gagcagagat acttaataaa tcgattccta attcttctgg atggatcgtt
    16741 tggtttccgt acgcttgtac cgcatctgta tggaaataag ctggatgatc cgctaaaagt
    16801 tcaccgattt ctttgattgg catcaaattt ccgatttcat tgttcccgta catcacagag
    16861 accaagatcg tatcttctcg taacgctttt ttgacctctt cgatcgacag attccctttt
    16921 tcatctacag gcagatatgt aacgtcaaac ccctttgtct ctaaatattt catggtatta
    16981 agaacagcag gatgttcgat agctgtagtg attagatgtc tgccttcttt ttgacgcgag
    17041 aacgctgttt ctaagatagc tgtattatcc ccttctgttc ccccactgtt aaagatgatc
    17101 tcgtgaggtt ttgcttgcag actgtctgca atgatttgtc ttgctgtttc caatttttca
    17161 tgcgcgtggc gaccaaatcc atgtatgctt gaaggatttc caaacgtgtc ttgcatgatt
    17221 gccgtcatcg cttcaattac ttgtggatgc atgggggtag ttgccgcatg atccagataa
    17281 atagttttca cttcattcat cccttccaaa ttattgctag gcacattcta gcataaaata
    17341 gcgaacaaac caaagaagag ctcatttttg tctagaggac gtttcatggg catagaaaaa
    17401 aggagctgtg acaaaagtct tgtcgcagct ccttgaagct gctcacttct gtcacaactt
    17461 ctttccctct tttttttgtt tattcgaatt ctttttcttc cagacggaat aacggactca
    17521 tcggtgtatt ttcatggatg cgtttgaccg cttcacccat taattctgaa catgtaacga
    17581 ttgttaagtt ttctggatga cgattttctt ctgtgaaaac agaatcagtg atacaaatat
    17641 ctttgattgg tgcattgtct aaaatacttt tcgcatgatc ggacaataat ccatgtgacg
    17701 cacaggcata tacttctttt gcgccgtttt cttttaatac atttgctgct cgtgcaagcg
    17761 ttgctcctgt gttcaagata tcatctacca tgatacatgt ctttccttga acatcaccga
    17821 tcacataacc accctcagtt gaagcatcat ctgcatgatc tacgatggct aatgtgctgt
    17881 tcaaatattc tgaaagacta cgtgcacgct gaacaccgct gttttttggc gaaacaacta
    17941 cgatgtcatc tcccgtcaga cctaattgac ggtaataatg agcaaacaac ggcatcgtaa
    18001 ataaattatc tacaggaata tcaaagaatc cttgtacttg aaccgtgtgc agatctaaag
    18061 tcagtactcg agttgcccct gcttcttcga tcaagttagc gatcaatttc gcagtgatcg
    18121 gttcatgagg tttcgccgtg cggtcttgcc tagcataccc atagtaaggc aatacgacat
    18181 tgatcgtttt cgcacttgct cgcttcattg catcgatcat gatcagtagt tccatatagt
    18241 aatcattgac aggtgcattc gttgattgaa cgatatacac atgatcccct cgaatacttt
    18301 cttcgatatt gatggaaatc tcgccatcac taaattgctt cacgacgctt ttacctaatt
    18361 ctgttccgaa cactttcgcg attttttcag ctaatggacg gttgccattc aaactaaaaa
    18421 tcttcaacgt gtcatcctga tacttattac tcatgtagtc ctccccaaaa cttctctatt
    18481 cttcgatagt ttaacatatt tttccaagat tttctgttaa tttcagcaaa ttcttaagga
    18541 agtgaaactg ttccgttact ccaactggca ggaatgactt tatgataatc gccccagttg
    18601 tcgtgtactt tgtaataacg ttctcctgtt tcagtttcaa aataagcgta cgctgtcacc
    18661 caatgattgc catatgtact gccaagtact ttcaaaatac caatcatcac aggtttgcct
    18721 tcacgtatac gttttgtagc tcgctgccat gaacctagaa gagttgctcg tgcacgctca
    18781 tgattgccat tcttctttaa aaaactagaa atccctgtac taacctgaaa aggaatcgta
    18841 ggaagtccta gtggttggat ctggctacgc aatgccttcg tcaaaaccaa cgaatcagcc
    18901 gtatttttct tgcgaatctc atttggaagc atccacccct tacgatagtc ttgataatag
    18961 gcaaggagaa ctgctgccgc gtaagtacca caaagatagc ctgagggggt agcccatttc
    19021 ttatacctca aaaaacgttc ttctggcaat cccacccagc aatttgcgag ttcctttcct
    19081 gacctttttt cttcgatcaa aacgggaata tcttcttttc tgttgatcca tctagataac
    19141 tcgattggct cttcattgat cagcttccat tttttacaag aaagaccctc ttgataagta
    19201 tattttgctg gagaatattc taccggatgt cctgattgat cgataatcac ataactttga
    19261 tcttcaaaaa ccagagctaa aaaaagcaat ttactttttc ttgactgcag ctgctgagtg
    19321 ctggctaatt ttttaatttt tttatctact ttgaaatata aaggtttatc cactacttga
    19381 ttcagttggg atcgcctcct ttttttcatt ataagtgaag gattgaaaaa aacaaatcag
    19441 tttgattgtt cgaattttca attttttatg ttctatttgt taattccttc attttgtaag
    19501 ataatgggca cacgaaacaa aaaaggagcg atcgatgaat gaacaaagaa cgattgaaac
    19561 aagatttcta tgaagcagtc aatgaagaat ggcaaaaaac agcaaaaatc cctgcagata
    19621 aaccagctac aggcggtttc caagatttag ttgacgggat cgaccaactt ttgatgggag
    19681 aaacagacga tctgcttgcc catccagaaa aagtgtcttc agaaaaaatg aaacatttcc
    19741 ttgcatatta tcaactggca aacgattatg accgaagaaa tgagttgcat gctcaaccgc
    19801 ttcaacctat aatcgaaaaa gttgaatcat tgcagtcttt cgacgaattg aataagcaat
    19861 ttccagaatg gctgttggat acattaccat tgccatttaa tctggatgta gatgcagata
    19921 tgaagaatgc gcaaacgaac gccttctttg cttatccgcc ttctttattc cttccagata
    19981 agacgtatta tgcatcagac catcccaacg gtgcacaact aataggcgtt ttctttgata
    20041 tggctgttca gttattagag cttgccggca aagaaaaaag tcaggcagaa gcaatcacgg
    20101 aacaagcagt gcaatttgat aaattgattg ctcctcatgt aaaaagtgca gaagaaaacg
    20161 cagattacag caaaatgtat aatccgcaat cttttgatac atttattaaa tacacagata
    20221 aactggactt gaaaaaactt gtcacgggtt tgattgggac aactccagat aaagtgatcg
    20281 taactgatcc agtgtacttc gatcatttga atgacatcct gacagaagct cattttccgt
    20341 tattcaaaag ctggatgatc attaaaacag tacgttctct tagtggttat ctatcagaag
    20401 aattccgcca agtcagcgga atctattctc gcgtattgtc tggaacagac gaagcaatgc
    20461 ctcagaaaaa agcagcgtat taccttgcct ctggacaatt cgatcaagta gttggcgatt
    20521 actacggaaa aaaatatttt ggcgaagctg ctaaaaaaga cgtagaacaa atggtgaaaa
    20581 aaatgatcgc cgtttataaa aaacgtctag aagaaaatga ctggcttggg gatgcaacaa
    20641 aagaaaaagc cgtcataaaa ttaaataact taggtatcca agtaggctac cccgataaga
    20701 tcccagcatt attcgaccag ttcaaaacag tacctgcaga tcagggtggt actttgcttt
    20761 caaatactct tgttttcagc aaactcacat taaaagatcg tttcagcaaa tggaacaaac
    20821 cagtggatcg ttcagaatgg gaaatgagtg ctgacacggt caatgcttat tatcatccat
    20881 ttcggaatgt gatcgtcttt ccggcagcga tccttcaagc accattttac agtctagaac
    20941 aatcaagctc tgctaattat ggtggaatcg gagctgttat cgctcatgag atttcccatg
    21001 catttgataa taacggcgct ttatttgacg aatatggcaa cttgaacaac tggtggacag
    21061 aagaagatct tacacaattt caaaacaaag cacaagcaat gatcgaagaa ttcgatggtc
    21121 tgccttttgc tgacggaaaa gtaaacggaa aattaaccgt ttcggaaaat attgctgatg
    21181 caggcggtct aagctgtgca ttggaagcag caaaaacgga atcagatctt tctatcgagg
    21241 aattctttgt caattgggca acgatttggc gtacaaaagc aaaaaaagaa taccaacaac
    21301 ttttactgca aatcgatgtc catgcccctg ctaaactccg agcaaacgtg caaccaaaaa
    21361 accttgacga attctatgaa acatttggta tcacggaaca agacgctatg tatctagcgc
    21421 cagaaaaaag agtgcgaatc tggtaagagg ttgtgccaga agtggtcagc ttcaagaaat
    21481 aagaaggaat ttgggaaaat cgcttctcaa atttttgtga catttcggct tatttttcga
    21541 agaagttact tctggaatac catgtattag aggttgtgaa aaagctgctt agtttcaagt
    21601 agtaagaaaa attttgtgaa aatggctttc cacatttttg cataaatttg acttactacc
    21661 gagaaactgg cttttgaaca ccgtttatcg agaggttgtg aaatcagcgt cctgacctga
    21721 gtattagaga aaaaaacgga aaaatagttc ctcatatttt gccgtttgtt gagctaatac
    21781 cgagggtcag ctgattgaac accgtttagt ggagagattg tgaaaaagct gtcctgcatc
    21841 aagtaataag aacagccaat caaaagcagc aaggagaacc tcatctataa aaagatagaa
    21901 ggaagtgaga catctattgg tctcatttcc ttctattttt actttcctca gtcaattgtt
    21961 ttacatcata aaaggagaaa ttccacttat ttttttcgct gtttcatgac ataaacaact
    22021 acaccgatta acaaaattcc tccagctaat gctagcaacg ggttattaaa ttctcccgtt
    22081 tttgggagtc cttgagtgcc atttttttca ctttcttttg ctgtcctatc cggatgagaa
    22141 ggttttccat ttggttttcc atttggtgat tcatttcctg gattttctct ttctggtttg
    22201 tcatttcccg ggggcacatc ggcagtttct cgcacagtga tcgctttttg ctgggtaaca
    22261 gattctgtat gagcagacca tttttcccca tcaaatattt ccgaacgata agtgacttct
    22321 agcgtatatt ctccttttga aggtgctgaa aaaatagcgg tatgattaga aaattgagca
    22381 cttgactgtg tttcaacgat ttggatcttt tctgggaaat aacgcatatc ttcaaagaca
    22441 ggcgttcctt gatttggtgt gaaagttgac acaaaaactg taaaaggctt cagttcaatg
    22501 atatttcctt ctgcattctc cgccacacca tttgcttctt gtataataat cggattattt
    22561 tgttcatttg gttttgtctg atttggttct tgtacgattg catactctgg atattccgaa
    22621 gacggtgagg tttctccgtc aaaaatagca cctacagata atggaagacc atccactctt
    22681 tccttttctg tttgacgatt ccccggttta atctgcatca gtccatgaac atggatactt
    22741 ccattttcat agcgcgtaac aggctgctga ccttctttcg aacatgtatt tagagactcg
    22801 atccaatctt ttgatatggt atgtccaaga gtattttctg attgtttctc tcgttcattc
    22861 caatagtagt tcaacgagtg gtagatttga tcttcgtttt gattcaatgg gcggatctgc
    22921 tctctcatct caagattctc tgttcggaaa gataagatac cagaagcatg gtagtctgtg
    22981 tttcctgctg aagaggtata aagagccaca ttatatgaac cgttattaaa acttgtactg
    23041 ctattgacga taatatctgt tccgctattg gaatcgattc ctttcgcaag attttcgaaa
    23101 gacaaactat tgatcaattc atgagggccc gttaaagaag aaccgcccat tttgaatcca
    23161 ttaccgttac cttctccctc aattcctggt acccagccgt ttcgataagc aactgagttt
    23221 ttgatgatta cttttccgat cgaacctgtt tcatttttag caaaaaggtc ccaaccgtca
    23281 tctgcattat gatacgcgat acaaccgtca aatacatttc cttctcctat tgtcagtttg
    23341 gccgcaaatc catcggcatc ttcaaatcct ggatcggcat tttcaaaaga tgtacagttc
    23401 ttgactaagt tatgcgcggg ccatgcttcg aaaggatcat ttgagctacc gctgatttgg
    23461 attcctgtat ttccattccg ataagcatga atctcttcga tcaaattatg atttccagaa
    23521 atctgtaatc ctttttgcat agctgcacta tttgtaatgt caaaccctcg taaaccccag
    23581 tagtgactga acagtgtcat cccatttcca gttcctttaa aatcaagtac aggacgcttg
    23641 ttttctggat ttttctctgc aatcaactga atcgggtttt ctcttgtccc gttaacgcct
    23701 tttggaatcg tcaatcctct ttcgaaggaa taagttccag gcgccatgac gattgtctgc
    23761 ccagcatatg cgtatctcaa tgcttgtgtc agagataatg gattttgtct gcttccgttt
    23821 cctgcatctg acccttctgg tgttacataa actgtcgaat atttaggttt acggtagtcc
    23881 actgtatgaa gaatctcttt tgtttcataa ctagtcatct ctacgtattc ttctggcgga
    23941 tagtttttat ctggcgtaaa actgaaagta aattcgttcg ttccttccat cagttttgtc
    24001 tgataatcaa attcccgatt cgcagtgata gccaagtttt ttgcgttctt aagcacctct
    24061 cctgttgctt tatttttaat tgtcaataat ccgtcagcat ttgctctaaa ggacatagta
    24121 tgatcttggt ttcctgaatg attactagag gtcacagtgt aaatcggatc aatcaagcga
    24181 gtaggtcttt cttgcccagg aggatctgtc gcaggatctg agtaagcaac ttcgattcct
    24241 tctaccttga tcgtcatatt ccttgctgca gcaaatcctg cataaataac cgattcatct
    24301 gaagcgtata atttttccca gtcatacatg atgtcagaag ccatttcttc tcctgtttca
    24361 ggatcgtaat aatatgcctc taaaccagta ttcgtttttt tcatctctac gtccagttta
    24421 gtaatcgagt tcgtggccgg aaacagattc ccatgaccgt ttccgaatat attataagaa
    24481 cctgtttctt tttccaagaa ccctgctgaa gtctctaaag gtgttgtttc cactgagaca
    24541 ctcccaggag ctggcgcagc ttgcggatct tcagatgata ttcctgtgat tgcccgtgtc
    24601 cccaatccaa gtcgcatcgt atatttgctg ccgctcgtgt tgaccacttc ttgtctatcc
    24661 gagtcccaga catattcgat ccgactgcct agtatggcaa atgaatttga ataaaatgcc
    24721 tgcccaaaca aatgattttt tggtactgcg tctcttacca ttaaagcaaa tccttcttgg
    24781 gcattcgtat aggtccaaga ttctatttcg atcgtcgccc gcatcctaaa gttgatgtct
    24841 ttggggattg cttgataata ataggtcatt ccatctgatc cgcttgactg gaattttccg
    24901 ccgttattcg tggaagagag ccgcacgccg tcttctaatg gttcaatggc atacctacta
    24961 gcacttgtgc tttctccgaa aacaacccct ttccactctt catttgcttc ttcagattgc
    25021 ttttttgtct tttcattttc tgattctttc atcataggat catttccttc ttccgtatcc
    25081 atatcttcac tagttcttgg tatttcctcc attgttgaca tcccttcaat gttcacgtgt
    25141 gcattttctt cgttcacaga ctgttgttcg ttagcagcaa cttcaacgac agaaattaaa
    25201 accggggtac tgagcataag gagattcatc attagcgcac tgtattttat acgttttttt
    25261 aacaagtgta ttcctcccgt aacaaattag aaagcgttta cttaaagtat tacctaattt
    25321 atttttactg tcaataaaaa acaaaattat aataagtgta ttttataaaa agaaaaaata
    25381 taacttaagt tgttgataat aaagagattt attaacgtaa aaagaataag tttttttaaa
    25441 ataacgtata aaaaagagca taaaacaaag tcgtttgttt tatgctcttc ttttattatt
    25501 ttgtgctttt tattttatca aatcactttg tttttttata aaatttctgc gcctagtgtg
    25561 gactcaaaat gtctcaatgc ccattcatgt ccaacgggat cgaaacttgc cacggcatct
    25621 ttgaaaatat ggagtttgta tcctagatta tacgcatcga ctgctgtatg caacacacag
    25681 atatctgtac atacgccggt caaataaata tctgtgatct ggcgttctcg caaacggatg
    25741 tctagatctg ttccactaaa agcggaataa tgccgtttgt ctatccaata aacattttcc
    25801 tcttgctcat agtcttggta taaacttttt aaagagccgt acaattcccg tccacccgtt
    25861 cctatcacat tatgcggagg aaataagcgg ttctccggat gaaaggaatc ttttggatca
    25921 tgcgcatcaa tggcaaaaac gacaaaatcc ttttgatcaa taaacttctt tgtatgagca
    25981 gtcagctttt tttcaattac ttgtcctgct gtcccagtag tcagctttcc atcctctgca
    26041 acaaaatcat atgtgtagtc aatcgagatc aatgcgttca tcttagtcct ccttatacga
    26101 gatgtttgac aaattcgatc accatttctg ctgatcgttt gcctgcgtct tcgataaatt
    26161 cgtcaaagct ttgtgtcgcc gagtgatctg ctgtgtcgct cattgcacgt acgatcaaaa
    26221 atgggatatt gaactgacga gctgtttgtc caacagcggc tccttccatt tcgcaagcta
    26281 atgcttcagg gaaattcgtt aagatttctt tgattttatc tggcgagtcg acaaatgtat
    26341 cacctgtaac gatcaatcct tcttttgcgt tcaaattcgt tgcttttgct gctttaacca
    26401 tttcactgcg caaatatgtg cttgcttcat aatatagagg cattcctggt aattgtcctg
    26461 ggttgtagcc aaatcctgtt gcatctgcat caaaataagc tactttgtct gatataacga
    26521 tgtcacctac ttgtaagcct tcaccgattc cgccagctga acctgtattg atcaccatgt
    26581 tcacgccgta ttgttggatc aacagacttg tggtaataga tgctaagact tttcctatac
    26641 cggaacgtac gacgatcact tcgtggttac ctaatgaacc tgaaataaat aaagcgcctg
    26701 cgcgttccca tgataatggt tcgcttaggt tctctctcaa aatttttact tcttcttcca
    26761 ttgcgccgat aataccgatt ttcatctttt taattccttc tttctatata aatgcgattg
    26821 ctaaaaaaac cacacataac attaggataa caataataag agatttcatc aaaaaagagt
    26881 tccatttggt cgtcttttcc cgttcgctga tccttgtttt cgtgattggt ttattctttt
    26941 tgctttcttt acgataaaag ctggcaatct ttttttcttc ttgctgataa gctgtttctg
    27001 cttttcgctg tttttttaat gactcgcttg cttgtgcttg ttgtcgcttg cgaagttcgc
    27061 ttcgcgtaat aagcggtcct ttggccataa gccgatccct ctactttcct ttggtcaata
    27121 gttcccagta ttgaatcgca taaatcgtct ttgcatcaca aatcaactga tctttcatcg
    27181 cttgttgcgc ctcttcaaga gttagatgat acaactccag tacttcgtct tcatcttgtg
    27241 ctaaaggatt ctcgaccttc tctacccctt gtgcatgata aatatgaagg acttcatttg
    27301 caaaacctgg tgaaagatac atcgaagtaa gatggctcag cgacttcgca cgatagccag
    27361 tttcttcttc taattctcta gctgctgtca tttcgggatt ttgtccttct cctggatcga
    27421 tttttccagc tggaatctcc aaaatcactt tttctaaagg ttttcgaaat tgtttgacaa
    27481 gaagcagacg atcttgttcg tcaaaagcaa tgatcccgac accgccagga tgaaatacca
    27541 gttctctctt cgaagttcct ccatctggca aacgaacttc gtctacagca acatcaataa
    27601 tttttccgct atagatttct ttgcgactga ttgttttttc ttcaaattgc tcattgttca
    27661 gcatatcttc tcctgctttc tttgtaatca taactgcttt tttttcattt ttcaattttt
    27721 ctcaaaaact ccttattagt ttaccataga taaaccttag attttctcta aatatagaaa
    27781 atccttcttt agtccgatac tctttatagt agtccgtatg ctaaaataag tatgtaatcg
    27841 aataacgaaa agaattcaat actcaacgat aaatgacatg gagggtttat tatgataaaa
    27901 cgaaattggc cgtggcttct agcattggtc ttgctatttc tctttgtccg aaacaatagt
    27961 ttttcttttg ttctaggact tttattgata ggtgtagttt tagttttgtg gggactcttt
    28021 ggttcgcgca ggaaaaaggg aaaacaagaa gaaattcctg cactgactga cgaacttgaa
    28081 actcactatg aaaaaagcgg tatgacagcg aatgaaattt cattttttcg acaaacaatg
    28141 aatcaaacaa aaaatgagat caatcaattg caaatgaata tgcagcagac agccaaactt
    28201 aaagcaatcg atctccgcca tgatccagtc aaagcagcga aagctttatt caaagaattg
    28261 gtgaaagaac caaaccgact gcatgaagca agtcagtttc tttatacgca tctgccgaat
    28321 ctagttgatt tgacaaataa gtataatgag atcaatgacc atgagatcaa aaacaaacag
    28381 acctatgaaa aactagaaga aagcgcgcag atcatcgacc agctgtcagc tttaatcgca
    28441 caagattatc aaaaattcgt tgctgaggat ctagatgata tcgacgtaga gatatctgtt
    28501 gcgaaacaga gcttaaagcg tgataataaa tatgaaccag aacaaaagga ttaactcttt
    28561 tgttcataaa agaagactcg gaacattcag atgatgtctg gctgtttcga gtactttttc
    28621 taaagtagag acattcatgt aagaatcgag aacagattca acaaggagga attttttatg
    28681 gatcataaac cgactgaaac aaaagatgtt acaccagtta acgatacact tgaagattta
    28741 ttgaacaacc cgttttcaac tccaacagat acgctgacca cttcacaaca agcggagatt
    28801 gatcagctcc aagagcaaca gacagctgct cgtttaattg acaaactacc ggcggaacgc
    28861 caagaacaag ctaaacagct agcagcaaaa atcgatgcta atgatgcaca gtctgtcatc
    28921 agctacggat cggctgccca agcaaaatta agtgaattct cacaatcgat gttgaatcat
    28981 gtgcaagcac aagatattgg tcctgtagga gattccttaa ctgaactgat gtatcgtctc
    29041 caagaagcca atcctgacga actgcgtgcc ggagaaggga acatcttcca gcgtgtcttc
    29101 gggaaggtca aacaatcgat ctatgaagtg acagcaaaat atcaaaagat tggtgcacaa
    29161 attgacaaga tttcagtgaa attagataaa gaaaaagacg gtctgctaaa agataatctg
    29221 atgttagaac agctttacca gaaaaacaaa gactactttg atgcactgaa tatttatatc
    29281 gctgctggag aattgaaaat ggaagaactt caaacaacga tcattccaga agcgatgaaa
    29341 agagcagaag aaactggcga tcagatggat gttcagattg ccaatgatta cacgcaattc
    29401 ctagatcgtc tggataaacg tacgcatgat cttcgcttag ctagacagat cacgatccag
    29461 caagcaccac aaatccgttt gatccaaaac acaaatcagg ccttggctga aaagatccaa
    29521 gcatctatcg ctacagccat tccgctgtgg aaaaaccaag tcgtgatcgc attgaccttg
    29581 cttcgtcaaa aagatgcagt aactgctcaa cgccaagtct ctgaaacaac aaacgactta
    29641 ttgaagaaaa attcagaaat gctgaaaatc tcagccatcg aaacagcgaa agaaaatgaa
    29701 cgtggaatcg tggatatcga gacccttcaa accactcaaa atgatttgat cgaaacaatc
    29761 caagagacat tgcgtatcca aaaagaagga aaagaaaaac gtcgtcatgc agaagtagag
    29821 cttggacaca tggaagaaga tctgaaacaa aaactgttgg atttaaccca atagatcacc
    29881 tttcgcttgt tactaaaaca aacgcccaac aacagtttat tacaactgtt gatgggcgtt
    29941 tttcttatct ttcttctaac ttctccattg gagtctattt ctcattcatc ttgctgtgtc
    30001 catagccata gccaaaataa atgatcagtc caataaccat tacgataccg aacacgatcc
    30061 atgtcacagt ttgcagacga agcatcaata caatacaagt caagatggag acgattggca
    30121 atagcggaac gaatggcact ttgaattctc cagcttttgg tttgccaaac atttttctca
    30181 acttcagtaa gcctagacta acaagcatca aataggcgag cgccatgata ttcgttagtt
    30241 ccgctaatac attcaatggg aaaaatccgg caaagaatac agctgccaca ccagcggcat
    30301 aagtcgcttt tttcggtgta cgatgctttt gatcgatttc tgtcataaac ttaggaagta
    30361 agccatcctt gctgacagca aacagtaaac gagcaagtga atacatcatc gagatcgtta
    30421 ccgtcagtag cgttaaaatt gcccctacag cgatcaccgc gcccactgtt ccatgtccta
    30481 catacctcat tgcaaaagcc actgggtcat tgactcctag ttcagtatac ggaacgattc
    30541 ctgtcaagat cagcgtcacg atgatgtaaa ggacagtagc aatcagtatc gaaccaatga
    30601 ttcctcttgg cacatctttt tgcgggtttt tgacttcctc ggctgccatt gcaacggcat
    30661 caaatcccaa gaaagcaaaa aacacaagag ctgcaccatc tagcacaccg gaaaaaccaa
    30721 aaggcatgaa tggctgccag ttatcaggtt taacgaaaaa tattcctaca atgataaaca
    30781 acgcgatgat accaaatttg acatatacca tggcattatt caaacgtaat gctttcttgg
    30841 cttcaagcga cacgatgtaa gtgacgaata tcactacgag caccgcaatc aagtcaatgt
    30901 atgtaccatt ttccggattg tacccgccag ttaatgcttg tggaagttcg gcccctaaac
    30961 ttttcaaaaa accttgcata tagccagacc atccggaagc aacagaggaa accgctagta
    31021 aaaattcccc gatcaacaac caccctgtca gccaggcagc aaattcacca aatacaacat
    31081 acagataggc atatggccca ccaatcactg gcactcgcga agcaaattct gcgaaactta
    31141 gtccagacaa aatgacaacg attgcagcta agacaaatga aagtgacagt gctggaccag
    31201 cattttgatt ggccgcaacc cctgtcacca caaatattcc cgtaccgatg atcgcaccaa
    31261 ttccaagcat gatcaaatca gacgttttta gttctttttt catctcactc gtatgattga
    31321 gattgatctc tttcttacga aacaaatcca ttgaagttcc tccccatttt aggttggaaa
    31381 acagcgatta aactgttttc atatacccta aaaggatacc acctaaaatg actaaaaggc
    31441 aaccaacaat aacataaatg agctcttttt tcgtttttct ttcgccaagg atgaaaatcc
    31501 cacctaaagt agagataatg atccccattt gtgaaagtga gaagccaaca gctaaaccaa
    31561 gtgatttgac tgttagaagc atgcagatgt ttcctaagcc ccataataat ccacagatca
    31621 tgtttttcca cacaaactga tcgactttaa ctttacggaa agcaaagaag cttgcgccta
    31681 agatcattcc gatactttga ggtaagataa ttgacatcgc atccaaattc gcccagttga
    31741 cgatgatggt atatacacca taaccaatcg tagagaagat caatgctcta aaacccttac
    31801 caaaatcaag cattttatta tctgtatcta tttttccttc gtcatcttgt cgggcagtca
    31861 aatacgcacc gcttacaaga agagctaaag caataatacc gtatacataa tctttcgttt
    31921 gtgtccattc atggaagaaa acggctcccg caatcgtatt caaaattaat tgaaaaccag
    31981 tagacaatgg tagccctaca gaaacaccca tgtacttcat tccatggaac tgtccatttt
    32041 gtccaacact ccaagctaag ccggataaaa atccgatcaa gaaaatccaa ggggtgatta
    32101 caggactaac aacgaaaaag acaactaaag aaaacagaaa tgcaccgatc gtcattccta
    32161 gtgtttgttg attggcatcg ccaccaattt taccacttac taaaccaata ctaccccatg
    32221 ccaacatagg gactaatgct attaaaattt ccataaccaa tctcctttta tttcacatga
    32281 taagaaactt atcagcgaaa acgttaacaa cacaagcact ttttttcgtt tctaacgatt
    32341 ttctaacgtt tttcattata aaaatttaag attgggaaaa ataaagtcat tgttgtccct
    32401 tttccttcag ttgattcagc cgtcaaatgg atacctaatt tttcggccag attttttgct
    32461 aaataaagtc ctaatcctgt tgaatgtgtt ttgctaagtc gaccatttct cccagtaaat
    32521 cccttatcaa agatccgttt caagtcttcc gctgggatac cgatccctga atctttaact
    32581 gaaaggctga ctccttgcgc ttccttttct atgctcacaa tgatgtttcc gtgatccggc
    32641 gtatatttga tggcattact aagcaattgg ttgaagataa aagcgatcca tttagcatcg
    32701 gtcaaaacag tatgatcctc accaacgatc tcgtagcgaa tatttttttg gataaaataa
    32761 tttgcctgac tgcgtatcac aggctgaatg attcctttta gtccatattc ttggatgagg
    32821 tagtcccgcg agaaactgtc gagtcttgca taatacaaaa cttgttctac gtattcatcg
    32881 atttttgata attcattttc tagtaatgta tacttttgat cgtcaatatc gaactcgatc
    32941 gaacgaacta gcaatttaga agcggaaagt ggcactttga tttcatggac ccatgagtca
    33001 atataatctt tttggtcttg ttgattactg attgctgatt gcattacttc ttggtgttca
    33061 cggatcagct gattgatata ttcttctacc agtttttctt cttctgtcct cccaccactc
    33121 aaataatttt gcaaagggga agttttttgt atgtcggcta attttcccca ccatcttctc
    33181 ttgctcaagt aaccacccag cagataaaaa atcagaaaga cgccctcaat caaagctaaa
    33241 taagcaattg tgctcaaatc aactttgatg tttggcgcca gccacatcac aaagatcgtc
    33301 aatgcaataa aaaacagcca tcccaagatc aatgtccact gatctttcaa atatttaaaa
    33361 aaattcatct taaccgctca tcctttcagg gaacgatata tccctgtcct acttttgttt
    33421 cgatatatcc ttggatacct gcctgttcga tttttcggcg taaccgattg atgttgactg
    33481 tcaatgtatt gtcatctaca aatcgttcgt catcccacag tgctcgcagc aatttttccc
    33541 gacttaagat ctttccattg tgcttcatca aaataaataa tagacgatat tcatttttgc
    33601 tcaaatcaat gatctctccg ttgatttcca tacttccgtt gtcaacattc aacgtaatgc
    33661 cattgtggct catcatttca ctagataatt ctgtgtagtt gtaagaacgg cgaagtaatg
    33721 cattgatttt tgcaatcagt acatcgattt ggaaatgttt tgtcacaaaa tcatctgccc
    33781 ccatgttcat agccatgatc atatccatat tcgtattcct gctggagata aaaatgatcg
    33841 gtactttcga tacttcacgt attttttgac accaataata gccatccaaa acgggaagat
    33901 taatatctag taatacaagc tgtggttctt ctctttcaaa gtctgagaac acttgattga
    33961 aatcggtcgt tccaaaggtt tcaaattgcc atttctcaag ttcctccatg atcaattgtc
    34021 gaatgatctc ttcgtcttcg actaccatga tttttgccat aaaaaatccc tccatctttc
    34081 ttcttgctac tacaatatca aagaagccct tgtgaaacaa gttggcttac ctggaaaatg
    34141 tcattttagc aaacgttaaa aaaaactgga aatatgcgat ttaatcgcgt ttgtctcttc
    34201 caagcagctt aaatttatta tgttattctt aataattggt ctattatctg ttatttttct
    34261 ttcgttgttt ttgtctttta aattttcgat aacaccaata accgaaaagc gttagcacta
    34321 gcaatatacc tagaaggagt ataagtaatc gataaagatg gtattttttt gtctgttcta
    34381 tctttgaaga gacttcttct gtaaagggaa ctcgtcttcc tgtcactagt aatcggtgag
    34441 tgttcacggt gtaaggtgta caagtgataa gtgtgactag atcctgcttt tctctaattg
    34501 ctaatgtatt tgtttttgtt ggaaggacaa caattttctc aacgacttca taagcgagca
    34561 tccgaccata cacttcaaga tagaatcggt catcttgctc catttcttca agatccgtaa
    34621 agagtttttt ctcagcaata ccgctatggg cggtaatcac ggaatgtgta ctctctcctc
    34681 cagttggaaa agaggtgcct tgcaggactg tcgctcctct atctaacaaa agatcattcg
    34741 tttcatcaaa taaaggcaag ctaacattga ttttcggaat atagatggct ccgatcatat
    34801 gttgttcgta atattctttt cctggatttt ggacatttct aaccgcttgc tcaaaaggat
    34861 ctttgacttg tcccattcct gggattactg atgtatgagc aagcgtctga tttttctttt
    34921 catgcactgc taattgttgc tcatgttttt cttctttatc gcgttcaagc tgcttttgat
    34981 aattctcgat ccttctttga tccaaataat tgctgagagc atctgctaaa aatggatatg
    35041 taaaaactgc agctccggca agaaacatca ctgccataaa taattgtaat aaaagactga
    35101 tttgggtttg aggtctatta cctattttct ttttcatttt cttcaccttc ttatttaaaa
    35161 aaactgcagc aacctttata aaaccgattg ctgcagctta tttttgttgc ttatacttca
    35221 gacttcattt tagatttacg gtaccagctg tacgctccaa tcatcaaact tattccgatt
    35281 agtaagaatg ccaagagccc gttcccacca gtagcaggaa gtgacccgcg tttcatattc
    35341 ggtactcttt ctggccctgg attttctact cctacgatct gaatttcttc attatagctt
    35401 ccataagaga tgataaattc agtaaaggct gcctcacctg ttggaagtac ataaccttct
    35461 ggcgccatgg tctctttcat ttgataggtt ccataatcta acccttgaac ctcaaagtat
    35521 ccatttccat ctgcgacaaa ttctgttgct tgtccgcttg ttgcccactc aattctatct
    35581 gctcctgatt cataaacagc taaaggcgac tcccctttat aaaatattgc gtattcagaa
    35641 cggtcttcat tccacaattc gaatcttgca tctttaagca attcactgct tgatgcatca
    35701 tatttttcaa actttcttcc acctgttgtg actttctctg gcggctctac tccttcttca
    35761 taatcaaatt catcatttcg tccgatttca ataaccgctg tgttgttatg gaaatcatcc
    35821 gcttctagtt ctgtggaaac cttcaaatta tacgtgatcg ttaaatctcc tccacgtgca
    35881 tctgctaatc tacctaaagt atctactttg gctactccat tttcttctgt caaaatcgtc
    35941 acgacaaacc cgttgcttac tacttctact atataatctt gtccttttgt taaaatcgtc
    36001 tcgccggctc gcacttctat ttcctgatca taataagtta gaccaactgt aggcgtatct
    36061 gtgattttgt aatttttgat aacagcgctt ccgttttcaa ggacagaatc gatgaaataa
    36121 ggaataggtg ctgtgatcgt gtaagaagcc gtttttcctc tttctagatc aatataagaa
    36181 atcgtttctt gttctccagc ttcattaata atcgttactt gtctaaggtc gctttcctct
    36241 acattcatct gtttatctgc ttctctgatt tcgtttttcg gatacaaatg cacatctgta
    36301 ttgtatgtgt ctttccatgt agaaccatcc cacacgccat ccaccatatc aggcatcatc
    36361 actggcatca taacaaccat tggagacgcg attctggaaa tatgagctgg cgaataagtt
    36421 tctaagaaaa gatagacttt atctctagaa gaaggtgctt cttccgtcac ttggacggta
    36481 gagaataccg cttctccagc ttcattcgtt gtttgtttgt caatacttgt cagtcgatat
    36541 tttaaaaacc aactttctgt gtgccgtttt tgaatccatt caatcgcttc ggcagaatta
    36601 agaccgtctt ctcgttccgg tgttaaagga ttatctcgaa tcaaatcgta atatacttcc
    36661 gttacgtcaa acaaatcaaa atcgactcct ggaatatttt cttcaccaaa atccgtagaa
    36721 accaatccgc tattcggtcg ttcttccgga acagatgaga aacctttttt gtgtaatgta
    36781 atcgtcactt cacttggttt ttgtgcgact tcttcggctt tgactagttt tccgttcccg
    36841 aaaatcccta ttcccgcagt aacgagtaac agtacactca ttcccgtgac aatcattttt
    36901 ttattcattt ttttctcccc gttctttttt tattaataaa acagcaccta acaataaaag
    36961 aattcctagt aaagaaatca acgttcttgc ttcattcgtt tgcgggaata cggaccattt
    37021 tcctgattga cctggattgc ttggaccact cggctctttc ggtttgtccg ttgtttcttt
    37081 tacggtctcg ttataaacac gtggtgttgc ggattcatag gcagagactg gaactttccc
    37141 ggattctaat tcagccatct cctgtccgtt aactactaca tattgtggtt ggccttttgc
    37201 atccacccaa aattcaggga tgatgacctc gattctctta cttgcttcat ccatctcgta
    37261 accttccaca ccttttaatt cttcaaaata ataggtgcca gaaggcaaga aacgttgtcc
    37321 tgtcgtaaca aggccctctt tatccgaaat gaatttgctt acatttttgt cattcaaagg
    37381 atcattgtct gctggtttga tccattgatt tttcaagtca ttagcaggag ccatatctaa
    37441 ataatatttc cccccttcta tcatctgata tagagcaaaa acagcacctt ctaatggttt
    37501 tcctttttca gttgttcctc tttgacgtcc atacttgaag aagtatggat ctcttagata
    37561 accgacattt ttaggataga tatgaatctc ttgcagttcc tcggttgatt ctgtcgggtg
    37621 ataaattggc aaaatagcag caattgggat agctgtctga tcgatatcaa catccaatcc
    37681 aacttctgga tcaatcgatt cttcgaaaat caaataaact gcatcccgat cttggttttt
    37741 cttaggtgct ttgatacgtg caatcccgtc tcctaatccg ccaaccgtat ctttcccagt
    37801 tgttaccgaa gtccctattt ggacaagcct ttcttctgtg attagctgac ggatattgtt
    37861 tctgttcgtt tcagcgattt gctggacgaa ttctttagga gtcatgtttt cagcagcttt
    37921 tttttcatga taataatcgg tcatgtcgta aatggcaaat gtcgctccat ttagtggcac
    37981 cgtctgtgaa atgatatctg ctgtttcccc tgcttcctca gagatagcca agccatcatt
    38041 ttcatggtat tcaaattgat cgttataatc gatatctcga atcatccgtt tatgcaacac
    38101 gaattccacg acatcttccc ctgattcttc tgccgacatc gcatgtggaa gaaggactgc
    38161 tgcaagcggt agtaaaacaa gtaattgaag catcaaacac aacagctttt tcattacctt
    38221 gctccctcct ttttagattg tctgacaaac aaaccatagg ctgctcctgc aagcgccatg
    38281 aagaatgcag caagcaggat atagaataat cgtccggatc ctcctgtagc aggcaacgga
    38341 ttttcaggat cgttcccgac actaagctgt aatctgattc gatttgtctc caaaagttca
    38401 tattctaact cttgctgtag atcaggcgtc tctgcttctt cgtttggata atacgaaatc
    38461 tcaactttta gtgtacccgc ttcttttcct ggaactaccc gatcattttc tatgtcaggt
    38521 tcttctcgat aactaatttc taaaataaag taacctggaa gttttctata gctgtcaggt
    38581 gacttcactt ccttgatccg atagacccct tccaacaagt ctgaagcatt gaatagggaa
    38641 gaatcgtttg gatctggtgc caagcctccc tcgatgattt catactctcc tgcactattt
    38701 tgtttctcaa ttgaaaattc tgcgccttca agacgtcggc tattcatact gtcaattttc
    38761 aaaagttcca aatcgattgg cgctagttca tcttctaagg ttacttgtag tacaccatcg
    38821 atgatttcat agccttccgg caagggagca gtttcgtctt cgatgaccgt atcttcaatg
    38881 ataaacttac cttcttcatt cagtgcaaac tggaacactt gatcagatag ccgataccca
    38941 tttggtgcga cagattccga gacctcatat tcaccaatag acagatgcag aggttggtcg
    39001 taatttaaat cgacatactt ttctactcct acggcacgag aatagaagta tccgatccca
    39061 ggcaaacgtt tcgattcttc tggttctaac ggattaccag tgtatgctcg atacagcggg
    39121 aaggaagaat cccccacttt cctgatatcg aaacctgcac catctaatgt atggccttca
    39181 tggtctaatt ttctaactcg gatctcccga taatcaaacg tattgtaagc agtgaatgct
    39241 acccataaac gatcctcttc atctgttgta tacgtgagat attgccaatt ttcttcacct
    39301 gtttgcttga gttggatagt ccctatagca ttatcgtaaa gtcgcaagat ccatgtgtca
    39361 ttttcatcag gttctttgaa accttctggg ttctggctct caatgatttt gtattctgca
    39421 tagttggtag tttcttcact atccttataa acaaatgaat ctggctctcc gattggatga
    39481 ataactttct caccatccac gattacatat ttcccgtctt catctactga atagaacgtg
    39541 agtctccctt tctcttcctc tacttcgttc acaccagaaa gccctttcgc tactggatat
    39601 ccgatttcca gtccgttacc atctgtttca taaagttcaa actctgcgcc attgattggc
    39661 atattactgc cttcaattag tttattgata ttcatgtgga ttggcttgaa ttcatttcgc
    39721 atttccagtt tgacatacaa ctcatcatac tctgtgcctt ctatgttagg ctcttgtaca
    39781 acagaaagct tgtgatattc tccttcataa taggaattcg gatctttacc ttccggtccg
    39841 ccgactctcg aaccagatgt atattcatct acaagctcgt agtgacctaa catctcatct
    39901 tcgttggctg catatttttt gatttggaat tgatacgtct catctactaa ctgataacgt
    39961 ggtggtccag cgatctctct gatccaaaat atgtcgctga ctgctccagt ttcatctcct
    40021 aaaatcggca tcttcgtttc aaagatcgct attccgtccg tggtagtttt acttgtttcg
    40081 attggttcgg cttccccact ctctggattt tggtcatata atgcaaattc gactccagcc
    40141 aacggctcgc cattttgacc cttcttgctg acttggaaat tacggccttt atgttcgtaa
    40201 cgaatggaag aagatgggaa atacatccgt ccagtagtga tatcattctc ataatcctcc
    40261 aggttctgaa tataggccat attgttcact gtgcaaaaat ttccgtcttg agctcccgca
    40321 tcaagtttta cttggtactt gatcctgacc atttcccctt cacctaaaga aatcggagaa
    40381 aatttataag cttggttttc ccgtccgaca tacgttgtcg gagtcgccac tacttctggt
    40441 ggggcttgtc catgacgttc ttcatcccat atttctggct cttggtactt catgccgttt
    40501 tctcctggat ctagatggta aaactgaata tctgccgatc tgccagctcc aaatggtgag
    40561 acattaaaac cagtttcatc ataaagtgtg gcttgctgga tcgttctctt gaaggtagaa
    40621 gagatatcct ccaaggctgt aacaatgtca ttcttgttgt tcgcattgta atatcgcggt
    40681 gttacgccat cttcttctaa atcagcaata tttttcaata catttcgtcc aatagcatct
    40741 agtcgttgtc tcccgctaac gtttccatcg attcctaaac caatggagaa gatacttggc
    40801 gggttctctt gatacttgtt tttcagccaa tgatgataag caactgtgtt gatcgcagat
    40861 gacttttctt gcgaagtagg actgccattc gtattgttat tagaagaacc atcaccaacc
    40921 cagtgagtcg ctttattatc tccttctttt acctctccat aacgatagtg tacatttact
    40981 tcatcttctg gacggttaaa gtcagatgca taggtaactg gatgagtaaa ccctgcatta
    41041 gaagtattcc cagaaaactc ctcaaaattt ctgaacaaaa cgtcattatc ttcagcaatc
    41101 ttatcttcca tcacagtcca gttgctccaa gctccattag tgctcgtacg ataacttgac
    41161 tgaatcggtg cataactgaa ggtcggtgtc ccatctccca caacgattag gattttttct
    41221 ggatttctat tttctccgcc attgtcttta tacaatgttt catagccatt tttaagccct
    41281 agtgtaagcg gagttcctcc gattggtgta cgatttaatg ctgtattctc acttcccgtc
    41341 aaacgattga tatcatttgt caatggaaag atgtccgaac tgatttgctc atggttattg
    41401 atagctgtac tattatggta aaaattcacc ataccgattc gaatatccag actcgggtca
    41461 gagagcagat ctttactcat ttcgtttact gcctcaatca acgcagcatt tttagtttga
    41521 ctattggtcc cagcggttaa ttcactcata ctcgctgact tatctagaac aaaaacgata
    41581 tcgattggac tgatttcctt tttctcgccc ccgatgacat ctagatagac attgtaaagg
    41641 gttgttgcat cttcaggatg tagatcagat gctgtgctgc ttggcagaac aggttctacc
    41701 cattttttga tgtacgcccc gttataatcc aagtaactgt tttcaaaatt ccgttcattt
    41761 ccccaaagct gaacggtttg agctggaacg tttctatctt cttcgcctgc agttgatcca
    41821 ttattagtag catagtcgta atttcgccag ttcgctcccc cgtttgtata atcactatca
    41881 tcgatcaagt tctctccgtc atatggcgag cctttgtctc cttgatagcg ttcattgctt
    41941 tgtgtccaat ttcctaacac ttttgtatag tcattcgttg taaacttcat ctattctttc
    42001 atcaaccacc acttgaagag gtagttaccc ttcgttcaca tcggggatct tttctgtttc
    42061 aaaaatcaat gtgccggttt ctgcttcttt tgaataaact tttccgacat accattcttg
    42121 accatcgatc gtctcgactt ttaggtcttc tccatcttca tcataggagg atagacctgt
    42181 tccacgaacg ttttttacgg taccaatccc ggcggctgcc gcatcgattg ctaaacgtat
    42241 tctaccttca ttggcacttt cctttttttc aaaagccaga tgccattcaa gggtttcttc
    42301 tttttcaata tacttccctt caatttttgc atagtcctca tctaagaata cctcatcctc
    42361 ctttgtttct gttgactcag cagctacatc cagggtcgtc atatgataga ctgactggaa
    42421 atatggtcca aatagcaaac tgacagttac taactgcaaa agcttcaatt ttaacccttt
    42481 cacatcctta tactcctttc aaaaatattt gtcttctttc acaacaatca acaaaacaat
    42541 gcccatctaa ttataattta atttaatttt aattttaaat caaatgatat cctttttagt
    42601 taaaaacacc aattatacaa catttattat taaaattaaa ttataaaatt ttataacgtt
    42661 ctcatttaac aaaacttaag ttattaagac aaatgaataa acaactacgc tttcctcgaa
    42721 acaaaaaaat aaagcacaaa tgcgcattta cgcactcatg ctttattcgt acaattaatt
    42781 cagataacgg cagcgcatag gatgccaata acacttccta aaatagctcc tcctaaaatg
    42841 tcagtaggat aatgtaaaag taaaaaaatc ctcgaataag cgatcaaaca agcaaataaa
    42901 aaggctaata ttcctgcaac tggacttaat tcgaacaacg cagctactga taaaaaggct
    42961 agcgctgtat gtcctgaagg aaaagaagaa gtcttcggtt tacttgtgta aaggacaagc
    43021 cgacgatttt tttcataagg tcttttccgt cttgtcgttt ttttgataaa aagcaaacca
    43081 acaaaaaaat agatcaaact gccgataaga atggctagac catattctct agtatcaggt
    43141 atcaagacta aaacagcagc aaggagcaac caaataaaac caccattaga gatattactg
    43201 ataaatttca aaaaattgat ttttcgacta tccgttttcc gttgcttggc gaacttgttg
    43261 ataatactat catcgatttt agcaatcaaa cgcaacatgt tattcctcct ttttaagaac
    43321 cttttcttgc gtacgatgga taaaaagcag gacttgaaaa taaaacaaga aactgaaacc
    43381 taataaagca aaatagattg gtcctgcaaa ccagaatgcg acaagaagga tcaacgtgcc
    43441 tgccaaatag aaaagcgcgc ttttgaaatc attaaaaaac aagaaaaatg cagtaatcca
    43501 agaagcttct ttttgttcca gttgaagcaa gattttgtaa ctaaagagaa gcgcgcttaa
    43561 tagaagcaaa aacaacgtag tatacatcaa tgtatagcca attcccccac cccaatagcg
    43621 gaaaatctga taatcgatca atagaagaag aaaataaaca cttgctaaac tatcttgctt
    43681 tccataaaga gagaagttct cacgaaactt tttccagaat agtgaaccga gctttttctc
    43741 ttcatttttt ttgtaatgat aaatcatata cagaactgtt cgacttgcag ttcctaatgt
    43801 gaaaacaggc aagacgcaca ggaaccatag tagctgtaaa tggataagac gtggaatcca
    43861 ttcaatgatt tctaagaaat aatcattggc ttttttacca atcgatcgat tcatcatcct
    43921 tttgaacctc ctgtcaaatc catcccttca atgaaataac gttgtgcaaa aaagaacagc
    43981 aaaatcgtcg ggatcaagac aagtgttgct cctgccatca gataattcca ttgtgtcgtt
    44041 gcttggttct gaaaaacttg caagccaatc tgtaacgtgt atttttcttg atcttggata
    44101 tataacagag gacctaagaa atcattccaa gctgcgttga aggaattgat cgcaatggta
    44161 atcaaggcag gtttcgtcaa tggaagcatc aaatgactcc aaatatacaa atgattcgct
    44221 ccgtcgattt cagcagcttc gatcaattca ttattgatcg ataagtaaaa ttgacgcatc
    44281 agaaatatat tgaacgcatt gccgaagaac gatggaacga tcaggggcaa ataagtacct
    44341 acccagccga tcttggagaa taatacatat tgagggatca tcgttacaaa ccccgggatc
    44401 atcatcgtag aaagaaccaa agcaaacatc agctttttcc caggaaaatc aagcttcgca
    44461 aatccataag caataaaaga atttgaaagg acattaccga tcactactag cacagtgatg
    44521 aataatgtat ttcttgcata gcgccaaaaa ggaaaggcgg caatcgtatc cagatagttt
    44581 tcccatacga ttttttctgg gaaaaaggtc ggaggataac gaacgatttc ttccattggt
    44641 ttcaatgaag taaacaccat ccaaagcaat ggaatgatca aaatgattcc tattactgtc
    44701 atgaggacat aacttagtat tttttttgtt tgccgattca cactggtctt tttgaaataa
    44761 tccatcattt gccgtcacct ccagaataat agacccactt aggtgcaagc ttcaaattga
    44821 caaacgtcag aaccaacacg atcagaaaca ggatcattga catagccatc gcataaccca
    44881 tatcaaaagc tacgaacgcc ttgttccaca tatgcagatt ataaaaaagc aaagaatttg
    44941 caggtccgcc tgttccattt ttcgtcatga cataagcttc ttgaaaaatt tggaaagaac
    45001 cgatcgtaga ggtgatcaca tcgtaaaaaa taataggagt gattaatggc aaagtaatac
    45061 ttttgaactt tccccaaacg cctgctccat cgatttctgc tgattcatat aaagatctag
    45121 gaacattttg taatgtagct aagtaaagta gcattgctcc tccagaactc catattttca
    45181 tcaaaatcaa cgcaggtttc gtccattgtg gatcaaacag ccaagctggt ccagggatat
    45241 ggaaccagcc cagaaatgtg ttgatcaatc ctgcagatgg agaaagcagc tgcatccata
    45301 agatgtagac tgctactcct gataagactg ccggtaaata gaaagctgta cggaatacac
    45361 tgattccttt gattttttga ttgagtactg tggctaaaaa cacactgaaa atcgtcgtca
    45421 gcggtacatt gaagatgacg aaataaacgg tgttgtataa cgatttccaa aataaatcat
    45481 cgtgaacgaa cattcttttg aaatttgcta gaccaatgaa attcatcttg gatgtcaaat
    45541 tataatcggt gaaactacca ataactgaaa aaagcatagg ccctagcata aataccaaga
    45601 acccgataat aaaaggcgag ataaataaat aggcccatct tgcttctttt ttctttcgga
    45661 tagataaacg cttcaaattt tcttgcctcc tctgtaccta gacagtcaaa aatggattat
    45721 tgattttgct tgactaagtt ttctactgct ttttgtgcat ctgccaatcc ttcttttggt
    45781 gattttgttc ctaaaagaat ctggtcaatc tgttcattca ctaatgtcgt gtaatccgga
    45841 gcatctaaag gaactggtgt gatcaccgta tttttaaagt tttcgtccgc catttgatag
    45901 atcatttttc ctttatccgt taggttgtca ttattcacta aattctcatt ggctgtcttg
    45961 ctcgccatga tatcaaaact tttttcgccg aatttttctt gtacttctgg agaagaaaga
    46021 tatttgatga attcataaga ggcctcagga tcttttgctc cgtatggtac ttccaagacg
    46081 aatccgccac cccacgacca atggcctgaa ccttcttctt tttctgggag ctgcatcacg
    46141 ccaaaatcaa aatcatccgg tgcattttcc atcaaactag agtaataatt gatattttgg
    46201 gcacgcatgg ctactaatcc ggaaataaat ggatcagata caccagaccc aaattcggct
    46261 tctaatttat tgatcgtatt tttcccataa taatcttgcc agtcttggat ccactccaag
    46321 gcttccactt tatttttagt atcaatcttg actttttcat ctttgtcaaa ccaactagtc
    46381 ccgttatctg cgtttagcgc ccatacatca gcccctaaat tccataatgg ataaaaaccg
    46441 atgcgtgtaa attcttttcc ttctttttta tctagtttac gtgctgtttc ttctaattca
    46501 tcccaagttg tcggtagatc ttgttcactg attccagctt ctttaaataa ctttttatta
    46561 taaaaaatga cttgtgtatc tgtattgaat gggatcgcat aaggatcacc ttcatattct
    46621 actgtatccc aaagctgcgg ataaaaatcc ttagaagtgt ctttttctac atatttggag
    46681 agattagttg cttgctgtgc ttctgctctt tgggcaacgg aatttatgtc ctgtacaata
    46741 acatcgggtg catttccagc tgttattgca gataaagatt tagtccagat gtcaccccat
    46801 ggctgatact tataatttac atggatctcg ttttgtgatt gattgaaatc ctcaatgatt
    46861 tcttcgatag cttcttttcg tgcaccggat ccccaaaagg accagaaatc aatatttttt
    46921 cttccatcac ttgttttttc agaagaattt gctgcacttg aactggcatc gcttgcatta
    46981 cttgatccgc atcctcctaa taaaaacaat gctgaacttg ccagcaaccc tgtaaacaat
    47041 aattttttca tattttacct ccaaatttta atgtaaccga tgccggtttt aatataacga
    47101 tatcattttg ttctgaaaat aacaatttat aaggtatgat tctaaaaaaa ttattaatga
    47161 taccaatact ttatataatt cgcgagcgct ttctttgaat tttttaatct ttcgtagcag
    47221 aatgagaatg tattaaaaat agattaggag gaattttgat gtttagacac cagaaagaat
    47281 tgcagtttga agcaaaacca gatcgtccga atccattaat cgcgaaatat ctgcaggagc
    47341 tgatcggcgg ccaatatggc gagatgagtg tagctatgca gtacctgttc caaggctgga
    47401 gctgccgagg agaagaaaaa tataaagata tgctgatgga cattgcaaca gaagaaatcg
    47461 gccacgtgga aatgttggcg acaatggttg ctcgtttatt agagggtgcg cctgttgaag
    47521 atcaagaagc agcgatgcaa aaagatccaa caatcggtgc cattatctct ggaatgaatc
    47581 cacagcacgc gatcgtagct ggcttaggac ctcgtcctgc tgacagcgtg ggcaatccat
    47641 ggagcggtgc ctatatcatt gcgagtggca acttgttagc agactttaga gctaatttaa
    47701 atgccgaatc acaaggaaga cttcaagtag cacgtattta cgaaatgatc gatgatcgtg
    47761 gcgtaaaaga tatgctgtct gttctccttg caagagatag ctatcatcaa aatctatggg
    47821 aatgtgcagt gaaagaatta gaagaaaaag aaggttcaat cttagtccca agtactttcc
    47881 cgcgtgacaa agaacgaaaa gacattgctt atgacttcta taacttctca gagggagacc
    47941 aaagtagtga aggttcttgg gctagtggca aagcactgga tggcgaaggc gaatatcaat
    48001 ggttgaaaga acctaagatc gaaggcgctg caccaaaatt agaaccagta acaccaaaaa
    48061 tgtatgggac acctccattc aagaaataaa tatctacccg tagaaacacc cgttttttgt
    48121 tccacacgct ttgtgagcaa aactgactaa agtcattaat cataaccacc cttcaaaagg
    48181 gtggttttct ctagagctat aagctcaaat caccggccag cgcctaaaga cgctggcttt
    48241 catattttat gttcaagcca tattgccttt gccagttttg cccacctctt aaaaggttta
    48301 tgtactactc cttagccctt aaaaggatcg tcttactctc gtacactcag cttatctaac
    48361 gcactatcat gtttttcttg ttcttgaatg tactttttga tggtcgcttc attttgtcct
    48421 acttttgtca catagtatcc ttccacccaa aagtgtcgat acccaaattt atattttaga
    48481 tttgcacaat ctcttgatga tctcctgaat attggctcga tattgattat aaattatttt
    48541 tcttctatac tttggtgtga atacaatgtg gtatttacac atctactttg tacgggctaa
    48601 actatttccc tttattgctc cacccgcata gcaagtggtt ttgtatttca atcgctcggc
    48661 gcgctcaatt aggtctaaag acaataaatc taaaaacccg ctgcttcact gacagaaaca
    48721 acgggttttc agaaaaaaat gaaatgaaaa agatctctta cggcaatcta ttttattttt
    48781 ttgatttttt ataaatggaa aatgcttcaa gaagtccaag aaataaagcc gtataaaaac
    48841 aagaacctat gaaatcttta tttattagat attcaacaga aatcccgata acactagcca
    48901 acaatatatt tatcagaagt gtaaaataag gatgcttttt atagaataga actagtgttg
    48961 ttttcataga ctctcctttc ttcatatttc caattaatca caaattcacg gcattactag
    49021 aaaaattaaa ggggcgcaga agtggaaaaa tactcttcga ctttccctac aaaccaggcc
    49081 atcttgtgtt taataactat accaaatcta aggtacctct aaatctaaat tccatttgtg
    49141 gcggtgacct ccaattttta actcttctat ctcccatcca gtatacataa ccttgatact
    49201 tcgaccactt atatatcgaa cacacaaata cctatccatt gcaggagtac tacctaactt
    49261 agttgctact tgcatctaaa taacagctga aaagatagga cttactctta gtaaagtatc
    49321 tgaactatta atttttaaac tatcattttt atcctcaact acatgcacat ctgttgcaac
    49381 ttcactttct gtctcatcca gataaatgat taaggaatcg ggaattgcaa tatcttcagc
    49441 tgacgctgga atagttgagc taaattagtc atcatatctt tattataaaa taaccaacag
    49501 caaaaaatac aatgaaaaac aaacttggaa ctaatgtatc attaataaat gttgaaaaat
    49561 tcagcaactc attactgtat gattttagca gcccaaccaa catataccct ataaaagcta
    49621 ataagatggt tagg
//
LOCUS       DACDIE010000014        48174 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.014, whole genome shotgun sequence.
ACCESSION   DACDIE010000014 DACDIE010000000
VERSION     DACDIE010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48174
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.014"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            40..1938
                     /locus_tag="IV250_000896"
     CDS             40..1938
                     /locus_tag="IV250_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HAP8768197.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(2268..2987)
                     /locus_tag="IV250_000897"
     CDS             complement(2268..2987)
                     /locus_tag="IV250_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAP8768198.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     gene            3324..3656
                     /locus_tag="IV250_000898"
     CDS             3324..3656
                     /locus_tag="IV250_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8768199.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            3643..5646
                     /locus_tag="IV250_000899"
     CDS             3643..5646
                     /locus_tag="IV250_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAP8768200.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            5665..6135
                     /locus_tag="IV250_000900"
     CDS             5665..6135
                     /locus_tag="IV250_000900"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAP8768201.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            6156..6740
                     /locus_tag="IV250_000901"
     CDS             6156..6740
                     /locus_tag="IV250_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAP8768202.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            6755..7741
                     /locus_tag="IV250_000902"
     CDS             6755..7741
                     /locus_tag="IV250_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HAP8768203.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            7731..8042
                     /locus_tag="IV250_000903"
     CDS             7731..8042
                     /locus_tag="IV250_000903"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAP8768204.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            8064..9845
                     /locus_tag="IV250_000904"
     CDS             8064..9845
                     /locus_tag="IV250_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HAP8768205.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            9838..11214
                     /locus_tag="IV250_000905"
     CDS             9838..11214
                     /locus_tag="IV250_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAP8768206.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            11221..11838
                     /locus_tag="IV250_000906"
     CDS             11221..11838
                     /locus_tag="IV250_000906"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAP8768207.1"
                     /translation="MARLNVNPTRMEQLTTATRGHKLLKDKQDELMRQFILLIRKNNQ
                     LRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVKVPVMNF
                     EYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASEIEKTRR
                     RVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            11868..13223
                     /locus_tag="IV250_000907"
     CDS             11868..13223
                     /locus_tag="IV250_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAP8768208.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(13415..14809)
                     /locus_tag="IV250_000908"
     CDS             complement(13415..14809)
                     /locus_tag="IV250_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP8768209.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            14901..15872
                     /locus_tag="IV250_000909"
     CDS             14901..15872
                     /locus_tag="IV250_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAP8768210.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            15869..16651
                     /locus_tag="IV250_000910"
     CDS             15869..16651
                     /locus_tag="IV250_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HAP8768211.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(16820..17191)
                     /locus_tag="IV250_000911"
     CDS             complement(16820..17191)
                     /locus_tag="IV250_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP8768212.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            17325..17768
                     /locus_tag="IV250_000912"
     CDS             17325..17768
                     /locus_tag="IV250_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP8768213.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(17811..18620)
                     /gene="yidA"
                     /locus_tag="IV250_000913"
     CDS             complement(17811..18620)
                     /gene="yidA"
                     /locus_tag="IV250_000913"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAP8768214.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(18639..20006)
                     /locus_tag="IV250_000914"
     CDS             complement(18639..20006)
                     /locus_tag="IV250_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8768215.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(19990..20820)
                     /locus_tag="IV250_000915"
     CDS             complement(19990..20820)
                     /locus_tag="IV250_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAP8768216.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            20983..21420
                     /locus_tag="IV250_000916"
     CDS             20983..21420
                     /locus_tag="IV250_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAP8768217.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            21496..22116
                     /locus_tag="IV250_000917"
     CDS             21496..22116
                     /locus_tag="IV250_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HAP8768218.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            22305..22811
                     /locus_tag="IV250_000918"
     CDS             22305..22811
                     /locus_tag="IV250_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HAP8768219.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            22828..23727
                     /locus_tag="IV250_000919"
     CDS             22828..23727
                     /locus_tag="IV250_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAP8768220.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            23814..24698
                     /locus_tag="IV250_000920"
     CDS             23814..24698
                     /locus_tag="IV250_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP8768221.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(24890..24961)
                     /locus_tag="IV250_000921"
     tRNA            complement(24890..24961)
                     /locus_tag="IV250_000921"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24926..24928),aa:Arg,seq:ccg)
     gene            25021..26355
                     /gene="rpoN"
                     /locus_tag="IV250_000922"
     CDS             25021..26355
                     /gene="rpoN"
                     /locus_tag="IV250_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAP8768222.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            26558..27595
                     /locus_tag="IV250_000923"
     CDS             26558..27595
                     /locus_tag="IV250_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAP8768223.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEA
                     AANEILKGVTL"
     gene            27636..28637
                     /gene="gap"
                     /locus_tag="IV250_000924"
     CDS             27636..28637
                     /gene="gap"
                     /locus_tag="IV250_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP8768224.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            28747..29937
                     /locus_tag="IV250_000925"
     CDS             28747..29937
                     /locus_tag="IV250_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAP8768225.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            30041..30796
                     /locus_tag="IV250_000926"
     CDS             30041..30796
                     /locus_tag="IV250_000926"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAP8768226.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            30912..32210
                     /gene="eno"
                     /locus_tag="IV250_000927"
     CDS             30912..32210
                     /gene="eno"
                     /locus_tag="IV250_000927"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAP8768227.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            32610..33749
                     /locus_tag="IV250_000928"
     CDS             32610..33749
                     /locus_tag="IV250_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HAP8768228.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(33816..34391)
                     /locus_tag="IV250_000929"
     CDS             complement(33816..34391)
                     /locus_tag="IV250_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP8768229.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(34396..35370)
                     /locus_tag="IV250_000930"
     CDS             complement(34396..35370)
                     /locus_tag="IV250_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HAP8768230.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(35394..35438)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            35653..36621
                     /locus_tag="IV250_000931"
     CDS             35653..36621
                     /locus_tag="IV250_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8768231.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     gene            36734..37012
                     /locus_tag="IV250_000932"
     CDS             36734..37012
                     /locus_tag="IV250_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP8768232.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            37130..37366
                     /gene="secG"
                     /locus_tag="IV250_000933"
     CDS             37130..37366
                     /gene="secG"
                     /locus_tag="IV250_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAP8768233.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            37478..38224
                     /locus_tag="IV250_000934"
     CDS             37478..38224
                     /locus_tag="IV250_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP8768234.1"
                     /translation="MRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSLAEQ
                     QLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLRWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            38227..40584
                     /gene="rnr"
                     /locus_tag="IV250_000935"
     CDS             38227..40584
                     /gene="rnr"
                     /locus_tag="IV250_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAP8768235.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWATELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKTRNGKSGKRE
                     GKQAGKRPERNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            40606..41070
                     /gene="smpB"
                     /locus_tag="IV250_000936"
     CDS             40606..41070
                     /gene="smpB"
                     /locus_tag="IV250_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAP8768236.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIIRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            41372..42091
                     /gene="atpB"
                     /locus_tag="IV250_000937"
     CDS             41372..42091
                     /gene="atpB"
                     /locus_tag="IV250_000937"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAP8768237.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMVISLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            42144..42359
                     /gene="atpE"
                     /locus_tag="IV250_000938"
     CDS             42144..42359
                     /gene="atpE"
                     /locus_tag="IV250_000938"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAP8768238.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVVALILVFGV"
     gene            42450..42974
                     /gene="atpF"
                     /locus_tag="IV250_000939"
     CDS             42450..42974
                     /gene="atpF"
                     /locus_tag="IV250_000939"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAP8768239.1"
                     /translation="MLNQLAIAEVGNAMLGNVIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            42961..43503
                     /locus_tag="IV250_000940"
     CDS             42961..43503
                     /locus_tag="IV250_000940"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAP8768240.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNKAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYAYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMNYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLENMRNQLNR"
     gene            43526..45082
                     /locus_tag="IV250_000941"
     CDS             43526..45082
                     /locus_tag="IV250_000941"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAP8768241.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            45096..45998
                     /locus_tag="IV250_000942"
     CDS             45096..45998
                     /locus_tag="IV250_000942"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAP8768242.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            46021..47427
                     /gene="atpD"
                     /locus_tag="IV250_000943"
     CDS             46021..47427
                     /gene="atpD"
                     /locus_tag="IV250_000943"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAP8768243.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            47441..47863
                     /locus_tag="IV250_000944"
     CDS             47441..47863
                     /locus_tag="IV250_000944"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAP8768244.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT        15921 a         8548 c        10305 g        13400 t
ORIGIN      
        1 tttataatat tgagtatacc aatttttgga ggcgttttaa tggaccgtta tttagattta
       61 ctttcagaaa aattcccctc aacggaggat gtcatcacgg aaatcatcaa tctcgaagca
      121 attttgcagc ttccaaaagg aacagagtta tttttgagtg atatacatgg cgaatttcct
      181 gcttttgatc atattctgcg tatcggttca ggaaatctta aagaaaagat caaagacctt
      241 ttttctgaac aaatgacgga aacggatatc agccaattca ctatttttac ggcttatcca
      301 gaatatgcat taacgactga ttggtacaaa gaacaagata aatcgcagct gatccatcat
      361 ttgatcgatc ttttacggtt tacgacagtc aaatataccc ggtcaaaagt tcgaaaagga
      421 ttgccgaaag aatatagcta tatcattgag gaactcttgt atatcgatga ccgtatcaat
      481 ggaaaaaaag actatgtaaa aaaaatcatt gaacaacttg ttttgatgaa ggaagaagaa
      541 aaatttttaa cgaaacttgc acagacgatc caaaaatctg tcatcgatca tttgcatatc
      601 gttggagata tttttgaccg aggaactcaa gcagccaaag tgatggatcg aatcatggat
      661 ttttcttctg tagatatcca atggggaaat catgatattt tgtggattgg tgcgtattgc
      721 ggctctgagg catgtttact gacactttta cggattgcag cacgctataa ttatttattt
      781 gaattagaga aagaatatgg gttgaattta cgtccattgt tttcttttgc tcatcaaact
      841 tatcagaaga atccagtatt tgcgcctaaa aacagtaaaa atctatccga aagagagcaa
      901 gaagaattag aaaaaatcca tcaggcactt tctatccttc aattcaaatc agaacatcag
      961 ttattggatc gtcgacctga atttaaaatg gacgatcgta aattgctgga aaaaatcgac
     1021 tatgaagcat caacgattga tttagaaggt cagctctatg agttgaaagg aacttgtttt
     1081 caaacgatcc agccagatga tccaaaaaaa ttcttggaag aggaacaaaa agtgattgat
     1141 agtttgatgt attcattcca gcactctttg agaatgcaaa aacatatgac gtttttgatc
     1201 gaaaaagggg gaatgtatct cacgaacaat cagcatgtgc tgtttcatgg ctgtatacct
     1261 gtagacgaga agggacagct cttagcattt gaattagacc aatcttatcg cggaaaagaa
     1321 ctgctgggct tttttgaaaa acagataact gaaagcgcaa aagatctgac tgcaaaagaa
     1381 gatttagcaa ccgatttgat ttggtatgcg tggagtgggc cattttcgcc tttattcggc
     1441 aagagtaaaa tggcgacttt tgaacgttat tttctcacag agcctcatac gcacgtcgag
     1501 aaaagcaatc cttattatca cttgcgagat gaagagtggt tcagtgaaaa aattttagca
     1561 gaattcgaca caaaagaaga agggtcagca atcattaatg ggcacacgcc agtaaaagta
     1621 aaaaaagggg agtcaccgat caaagcaaat ggaaaagtat ttgtcattga cggaggttta
     1681 tccaaagctt atcaaaaaac gacaggaatt gccggttatt cactattatg caattcttat
     1741 ggtttccaaa tcgtgacaca ttatccgttt ttgcctattg aacaacaatt tgcaaaaaaa
     1801 tccgaccaaa caaatgtgaa aaaagtaatc gaacagcaac taccacgcaa actcaatcgt
     1861 gatacaacaa aaggtatgga actgcagcag cagattaccc agctcaaaag gctgttggac
     1921 tatcgaaagg acgattgaga agaattccag tgaacattgt tccatagact ggagactgag
     1981 acaaaagtcg tgtcgcagtc tctttttccg gataaacggt gttcttctgc caactcctcg
     2041 gtcacaagtc acactattca acaaacatga aaagctgttt tttgaaattg ctccttgtga
     2101 ctcagagttg aacggcaggc tcacgacctc ttggtataaa cggtgttcaa aagccagttt
     2161 ctcggtatta agccaaattt atgcaaaaat gtgaaaagcc attttcacaa aatttttctt
     2221 aatactcgaa actaaacagc tttttcacaa ccccctcaga aagtctatta aaattcattc
     2281 acaaagaaat cgaataagcg ctgttcagat ggatgtacac ggtgggtaag ttccggatgc
     2341 cattgaacac cgaggatttt atgcatatca tctttgcttt gaaccgcttc aacgagtcca
     2401 tcgttagaaa aagcgatggc ttttaataaa ggtgaaagat ctttgactgc ctgatgatgg
     2461 taagaattca caaaatatgg atcggggaga aggttgctta acaaactgtc tgaaacaatc
     2521 ttgacttcgt gagtcgtaaa ttgcggggca gtgggctgtt gttcatgttt tactgaccac
     2581 tttggatata atgaaagatc ttgataaagt gtaccttcca aaacgacatt caatagttgg
     2641 agtccccggc agactgcaaa aattggtttt ctttgtttca atgcttccaa gatcagtgcc
     2701 tgttcaaatt ggtctcgttg aatatttgtt tccgttagct ttggatgagg atcttccatg
     2761 taaagttgtg gactgacatc ttgaccacct gcaagaataa gtttgtcgat ttgtccaatc
     2821 agttgaggag ctgaatctgg agacatgact ggcagaataa gaggcaatcc ttgagcatct
     2881 aaaactgcat ccacgaatcc ttgcggtgta tagctcactt gatttccttg gaaagtatct
     2941 gttgctcgaa tcagttgatt tccggcaatg ccgatgattg gtttcatttg tattccccct
     3001 aaagtagact tattgtaaca aggatttaaa aaaaattaaa ctatgtaaga cttgccccta
     3061 tacttatctg ttcttgctgt gttactatct gtaaagagaa atttcgtgtg aaattcatga
     3121 cgatagttga aaatattgct taatttgtta taataaaaag taagcagaaa taaaaaggtt
     3181 gggcgtaagc ttcaaggatc tagttcttca aggggtgaga aggacatttt attgaagggg
     3241 cctgcctata ctaagttatc tttagaggta catactttca atgaaaaaag aaagcatgta
     3301 cctctttttt aaatcgaaag gaagtgaagc aagtgttaga agctttggaa caaatccgaa
     3361 aagcagaaga agaaaatgaa aagcgtgctc aaacactgcg gcagcagctg aagatctatg
     3421 caacagaaaa agagcaggaa ttggaaacga tccaaacaaa ctatcgacaa caactaaaac
     3481 agttggtgga agaaaaagaa caatttgaaa tacaagaatt agaaaaagaa aaaatgaaat
     3541 tgaaagcaga agcagacaag gtgcaagcaa ctttgaaaga tcaatatcaa aagcatcaga
     3601 gtcaagcgat cggtgcgatc attgaaaggg tgaaagaaac ttatggccgt cactaagatg
     3661 gaaaaagtga ccctcatttc agataaaaga aatcaggaga ggattttaca agcagtacaa
     3721 ggaatccaaa acgttgaaat ccgggatctg tatcaaaata gaacaaacaa ccagtgggtg
     3781 gagaagtatt ttccagatac cacgattatt gataaagaag caaaattaag cgctttaggt
     3841 aatcggttga aagacatcca agaagctatc caattcatac aacaccacgg cgatagccgt
     3901 caaaagaaac tccatttgaa acgtcaagaa ctttccttac aagatttgga agccaattat
     3961 tccgaagaaa atttcataaa taaattgaaa gaagtccttg atttaaaaga aaagtgggaa
     4021 caactaactg aagaacgtga tcgtttagaa gaagaggaag aatggcttct tcattggcaa
     4081 cagctggata ttgctccaag tatgtatgca agtaagacga ccatttttga gatggggact
     4141 gtgagtgcag caaattttga aagtttcaag gaagaactga gtaagcttga tgacgtttat
     4201 atggaagaaa tagattatac gccaaaacaa gtttatcttt cgtatgttgc tttaaaaaaa
     4261 tcagttgctg ttatagaaga aattgctggg aaatacgggt tcaataaaga aacgtatcct
     4321 tatgaacaaa atcctaaaga aaaactgcaa gagaccaagc agcaattatc cattgttcac
     4381 gagcagcaaa agaagataac gacagcgtta ggtcagtgca gtggctatat ccaagaattt
     4441 gaatgggtag aagaagtcac acttgccgtt gcagaacgag aaaaaataaa agaacggttt
     4501 atccatgcga cttatctgat cgttttgcaa ggttgggtag atgcagaaga aaaacagaat
     4561 ttgctgcatg tgttaaacga aaccgtatca gaagaagata tatacgtcag tttcgaaaca
     4621 ccaaactcag aagagattca gcaggaagtc cctactaaat tgaaaaacca tccgcttgtc
     4681 gaaccttttg aattgctgac tgaaatgtac agtctgccaa aatatgaaga agtagatcca
     4741 acaccttgga tgacaccatt ttatcttgtc ttcttcggga tgatggtcgc agatgttggt
     4801 tatggtttgc tgatgctcat tgggacaata ctagctcaaa aactacttgt tctaccacgc
     4861 gggatgaaac gtttcgtaaa attctttgag attctctcga taccatcgat catctgggga
     4921 ttgatctaca gttcattttt cggacagtca ttaccaaaag aactgtttgg catccgcttg
     4981 cctttcccaa tcctatctac gacggatgac gtcaatacga ttttgatttt atccgtgatt
     5041 ttcggtctga tacagatttt ggttggatta tttgtggcag caaaggaaaa tatcaggaga
     5101 aaagattact tgagtgctgt cagtgatgga tttgcctggc aagggatcct aattgggatc
     5161 gttattgcgt tggttggtgc catgttgata aaaaatacag catttgttta ccttggcgct
     5221 gggctatcga tcattgctgc cttatgtatc ttgatcgttc cgattatcca aacgccgtct
     5281 aaagtcaaag gtgctgccaa aggtgcgtat aatctttacg gactgaccgg ctatatcggt
     5341 gatttagtca gttatacacg tttgatggca ttgggtattt caggcggaag tattggagcg
     5401 gcattcaata tgctagttgc tttcatgccg ccagctgctc gattcagtgt agggatcctt
     5461 ttgatcatcg ctttacacgc actgaatatg tttttgacac tgctaagtgc ctatgtccat
     5521 ggcgcacgat tgcagtatgt cgaatttttt gggaagtttt atacaggagg cggacgtgcc
     5581 tttgatccgc tgaaaacggc ggaaaaatac gtcaacatca atcacaagaa aaaaaacaat
     5641 aaataattgg aggattttta tataatgatg gattacttaa ttagtcaaaa tggtggaatg
     5701 gtatttgcgg tattagcgat ggcaacagcg acgatttttt cagggatcgg ttcagctaaa
     5761 ggtgttggaa tgacaggtga ggcagcagca gcattgacaa caagtcaacc cgaaaaattc
     5821 gggcaggctc tgattctgca attgcttcca ggcacgcaag gtttgtatgg attcgttatt
     5881 gcctttttga tttttatcaa cttaggaaat gacatgtcag ttgtacaagg actgaacttt
     5941 ctaggtgctt ctctgccgat tgcttttaca ggcttgtttt caggtattgc ccaaggaaaa
     6001 gtcgcagcgg caggtattca aattttagcg aaaaaacctg aacatgctac aaaaggaatc
     6061 atttttgctg cgatggttga aacatacgcg attttaggtt tcgttatttc cttcctatta
     6121 gtgttgaatg cctaagcata aaggaggaaa agcagatgaa cgccattgaa aaaatcatct
     6181 ctcagatgaa cgaagcagca gagcaggaaa gagcggcgtt agaacaagaa gagcgaatga
     6241 agatcgatca aaactttgaa caaaaacgta cgcaagttga aacagaacat caaaaacaaa
     6301 aagaaaaaca aatcgaatta ctcgaaaaaa aataccgtca gctaaggaat cgccagcaag
     6361 tagaagtacg acaagaaaat ttgaatgcga agcaggaatt tttaagacga ttgtttgcag
     6421 atgcggtaac tgaaatggaa aattgggatg aatctgaaca aatccagttt atcaaaaatg
     6481 cattgtattc attacctttg acaggaaaag tagcattcat tgctggagaa aaatcagcag
     6541 cctatctttc tcagacgttg ttggatgaat ggaacaatga acttcccttt atgatggttt
     6601 taagtgatga aactgttgct gatcaagcgg gttttctgat aaacgatcaa ggagttcagt
     6661 ataattttct tttcagcagc cttgtacaag atatccaagg aacgatgagt tttgagattg
     6721 ccaatcaatt attcgaataa ggagggatca gtgtatggaa taccacgaac taaatccctt
     6781 gattcgcgga agagagcttg aacttttgtc caatgagaca tttgaacgga tgattcaaac
     6841 aaattctgtc gaagcacttg gtgaaatatt gaaatccacg atttacagtc catatattta
     6901 tgacggatat gaatatgact tcgaacagaa tcttttaaag gagcagagcc gtctatttcg
     6961 ctggttaaag gaatcagcac cggagcctga aatcgtttgg atctatacga tgcgttatac
     7021 tttccataat ctgaaggttt tgacaaaagc tgagatgaca aatcaaaatc tggaccattt
     7081 gtatatcgac gatggatttt attcattaga aaccttgaag gatgccattc atacgcaagt
     7141 ttcaaatgaa cttcctgaaa gcattatgga cagtatacga gaagtacgtg attattttga
     7201 agagtctacc attcttcagg gaattgacgt gatttatgat cgacagtttt tgacagagca
     7261 gcgacggctt gccgataagc taggatatca agaattgctg acagaagtca ttgcttttat
     7321 tgacttgacc aatatcacaa cgatggctcg aggaatattg cagaaaagaa catatgggtt
     7381 catgacgacg gtactttcca gctcgggaag tatcgaaaaa gaagtatttt tgaactttgt
     7441 agataaagat atggaagatt acacgaattt tctattagag acagattatg ctgattttct
     7501 gcgccctgtc atccgtgagg gaaatattga tctcgtaaag cttgaacagt tgaaagatga
     7561 ttatctaagt tcattgtatc aagaagcaca aacccaggct ttcggaccac tgccactact
     7621 agcctttttg aacgcaaaag aggtagaaag caaaaacctt cgtttactgg ttatcggcaa
     7681 aagaagccat ttcgaatcag aaacaatcag aaagcgggtg agacaagtct atgacgcata
     7741 agatcggtgt agttggcgac aaagattctg ttttgccatt caaattgttt ggctttgatg
     7801 ttcgttatgg tacaaccaag caaaccgttc gtcaggcaat tgaagaaatg gcaaagaatg
     7861 agtatggcgt gatttatgtc acagagcaat gtgcagcatt gagcccagaa acgatcgaac
     7921 gttacaagga acaaatgata cctgctatcg tattgattcc caatcatcaa ggatcattag
     7981 ggatagggct ggaagaaatt caaaaaaacg tagaaaaagc agtaggtcaa aatatcttat
     8041 agtatgaaaa ggagatagaa attttgcaaa ttggaaaaat cataaaagtc tccggacctc
     8101 ttgtaatggc tgagaacatg tctgatgcca gtatccaaga catgtgtcta gtgggagatc
     8161 taggagttat cggagaaatt atcgagatgc gtggcgatgt ggcgtctatt caagtatatg
     8221 aagagacttc tggaattggt ccaggcgaac cggttcgctc tactggtgaa gctttatctg
     8281 ttgaattagg accagggatc atttcacaaa tgtttgacgg aatccaacgg ccattagata
     8341 cctttatgga aatcacacaa agtaatttct tagggcgcgg agttcaattg cccgcattgg
     8401 accatgagaa aaaatggtgg ttcgaaccaa cagtagaagc cggagaaacg gtgagtgctg
     8461 gagatgttat cgggatcgta gaagaaacaa aagttataaa acataaaatc atggtgccaa
     8521 atgggataaa aggaacgatc aaaacaatcg aaaaaggttc attcactatt gatgaaacag
     8581 tatgtgtcat tgaaaccgaa aatggtgaca aaaatttgac gatgcttcaa aaatggccag
     8641 taagacgtag tcggccaatc aaagaaaaat taaacccgga tgcacccatg atcactggac
     8701 agcgagtgat tgatacattc ttcccagtga caaaaggagg agcagcagct gtacctggtc
     8761 cttttggtgc agggaaaaca gtcgttcagc atcaaattgc aaaatgggcc gatgtggaca
     8821 tggttgtcta tgtgggatgt ggcgagcgtg gaaatgagat gacggatgtt ttaaatgaat
     8881 tccctgaatt gattgaccct aatacaggag aatcgttgat ggaacgtacg gtattgattg
     8941 ctaatacatc aaacatgcca gtagcagctc gagaagcttc tatctatacg ggaatcacaa
     9001 ttgcggaata tttccgtgac atgggttatg acgttgctat catggcagac tccacttcac
     9061 gatgggcaga agcacttcgt gagatgagcg gacgtttgga agaaatgcct ggtgatgaag
     9121 gttatccagc ttatcttggt tcacggcttg cggaatacta tgaacgatct ggaagagtca
     9181 ttgcattagg atcagaaggt cgcgaaggaa gtataaccgc aatcagtgct gtatccccgt
     9241 ctggagggga tatttcggaa ccagtcacac aaaatacatt gcgggtcgta aaagtattct
     9301 ggggtcttga ttccagttta gcccaaaaaa gacattttcc ttcgatcgat tggatacaaa
     9361 gctattctct ttattcaaca gaagtaggga agtatatgga tcatatcctt caagctgact
     9421 gggcatccat ggtcaccgag ggcatgcgta ttttgcagga agaacagcag ctgaatgaaa
     9481 tcgtgcgttt ggtagggatt gattcccttt cagataatga ccgtctgaca ttagaagtgg
     9541 caaaatccat tcgtgaagat tatttgcagc aaaatgcttt tgatgatgtc gatacattca
     9601 cttcaagaga gaaacaattc aaaatgttga agatgatctt gacctttggt gaagaaggac
     9661 gccgtgcgtt gtcgttaggt gcttatttaa gtgagatcat ggacggtacc gtatcgattc
     9721 gtgaaaggat cggacgaagc aaatatatcc cggaaacaga attagaaaaa ctagatcaaa
     9781 ttattggtga aatcaagcaa acgatgcaaa cgatcgtttc agaaggaggc atgagcaatg
     9841 attaaagaat atcgtacgat cggtgaagtc gtcggtccat tgatggcggt tgaaaaagtt
     9901 gctggtgtca aatatgaaga attaatcgaa gtccgtatgc aaaatggcga gattcgtcgt
     9961 ggacaagtat tggaagtcca agaagataaa gcaatggtcc aaatattcga aggaacaagt
    10021 ggtatcaatt tacgtgactc ttctgtacga tttttaggcc atcctttaga actaggtgta
    10081 tcggaagaca tgatcggtcg agtattcgat ggtttgggac gaccgaaaga taacggacca
    10141 gagatcctgc ctgaaaagca tttggatatt aacggagaag taatcaatcc agtagctcgg
    10201 gattatccag atgagtttat ccaaacaggt atctctgcaa tcgatcattt gaatacatta
    10261 gttcgtgggc aaaaattacc agtattttct ggctctggtc ttcctcataa agaattagct
    10321 gctcagatcg cgcgacaagc aacggtactc gattcatcag atgatttcgc cgtagtcttt
    10381 gctgctattg gaatcacttt tgaagaagct gaatttttca tggaagattt tcgtcaaacg
    10441 ggcgcaatcg accgttctgt catgtttatg aatttagcaa acgaccctgc aatcgaacgt
    10501 atcgctactc ctcgtatggc tttgactgct gcagaatatc ttgcttatga aaagggaatg
    10561 catgttttgg ttatcatgac agacatgacc aactatgcag aggcattgcg agagatttct
    10621 gcagcacggc gagaagttcc aggaagaaga ggatatcctg ggtatcttta cacgaactta
    10681 gctactttat tcgaacgagc aggaagaatc cgcggactaa aaggatcggt tactcaaatt
    10741 ccgattctaa caatgccaga agacgataaa actcatccaa tccctgactt gactggttat
    10801 attacagaag gacagatcat cttgactaga gagctttaca agagtgggat tcagccacca
    10861 attgatgtac tcccgtctct ttcgcggtta aaagataagg gaactggacc cggaaagaca
    10921 agagaagacc atgcagctac gatgaatcag ctttttgcag cctatgcaca aggaaaacag
    10981 gcaaaagaat tagccgtagt tttaggtgaa tctgctttat ctgatgtaga caagatctat
    11041 gcgaagtttg ctgagcggtt tgaaaaagaa tatgtcaatc aaggattcta cacgaaccgt
    11101 tcgattactg aaacacttga tctagggtgg gaactgctat cgatgttgcc aagaaccgaa
    11161 ttaaaacgaa tcaaagacga catgctggac aaatatctgc cagaagggaa gtgagttctg
    11221 atggcacgat tgaatgtgaa tccgacccgc atggaacagt tgacaactgc cacaagaggg
    11281 cataaattat taaaagataa gcaagatgaa ctgatgcgtc aattcatcct gttgattcga
    11341 aagaacaacc agctgcgtca agaaatggaa gagtctatga cagaagcaat gtctgatttt
    11401 gtattggcaa atgcttcgat cacggaacct ttcatcgaag aattatttgt tttgccggcg
    11461 gaaaacgttg atttgtctat cgtagacaaa aatatcatga gtgtaaaagt acctgtgatg
    11521 aattttgaat atgatgaaca gctgacagac acacctttgg aatacgggta tttgaattcc
    11581 agtgccgaac tggatcggtc aatcgatcgg tttactaccc tgcttccaaa acttttggaa
    11641 ctgacagaaa tcgaaaaaac ttgtcaactg atggcgagtg aaatcgaaaa gacacgtcgg
    11701 cgagtcaatg cattagaata tatgacgatc cctcaattag aagaaacgat ctattatatt
    11761 cgtatgaagc tggaagaaaa cgagcgcgca gaagtaacaa ggttgatcaa agtaaaaaat
    11821 atgggtacca ctgactaagt ggtatctttt tttaaaagag gaaaacgatg attaaacaaa
    11881 gcaaacaaat aaagaaacgt ttgccggctg ttcagcttct cgcattagga ttttttctcc
    11941 tgattttagt tggcggcggc ctacttaccc taccattttt tagtaatagt gggcatggga
    12001 caaattttat cgatgcgctt ttcactgcaa cttctgctgt ctgtgtaacg ggattgacaa
    12061 cattgaatac agcagagcat tggaacagtg ctggtcaatt tttgatcatg cttttgattg
    12121 agatcggcgg tcttggtttt atgatggttc cgattctatg ctttgcattg ataaagaaga
    12181 aagtcagtct cagcacacgt attatattaa aagaagcgct gaatctagaa gaagtatcag
    12241 gtgtgatgaa gctcatgctt tacatcctaa aattggcctt tgtgatccaa ttgattggta
    12301 cggttgcttt atcttttgta tttgtgcctg aatttggctg gttgaaaggt atctggtaca
    12361 gtttgttcca tgcaatctct agtttttgta atgcagggtt tgatttgtta ggagacagtt
    12421 tagccaatca ccagcaaaat gtttatttga tcatggtggt atctgctttg atcatttctg
    12481 gtgggttagg cttcatcgta tggcgtgatt tactggaata tcatcagaaa aagaagatga
    12541 cgattcattc aaaaatcgca ttaaccgtta caggtaccct tttattaggc ggattcgttg
    12601 tgttcttttt cacagagcgc aatggggcaa cgctagtgga agggaatatg tttcaacgat
    12661 tggcaaacac cttttttatg agtgtgacac ctagaacggc tggatattat tcagttgatt
    12721 acctccatat gagccatgca ggtctgattt taacgatgtt tttgatgtac atcggcggaa
    12781 catctgggtc gacggccggc ggattgaaaa caactacgtt aggtgtgttg ctgatccaga
    12841 tgcgttcgat gttgaaagga cgtacgagag cggaagcatt tggccgtacc atccgacaag
    12901 gcgcagtgtt gcgggcactt actttatttt ttgttacgtt gacgttgtgt gtagtcgcca
    12961 tcatgatttt atcggtgact gaaacgatac ctaaaacttc tggcatcgaa tatattgctt
    13021 ttgaggtctt ctctgcgttt ggtacagtag gtcttacgat gggattgacg ccagacttga
    13081 ctgcaatagg gaaattgatc attatagcgc ttatgtacat tggacgagtt ggtgtattga
    13141 ctgtggtctt ctctctgatg acaaaagcac aaagaacaca atcaaaatac aaatatccag
    13201 aagaaagtgt catgattgga taatttcatc ttctgacaga aacctagtga tttagtaaaa
    13261 taagaaaata aaataatttt tcttgcttct tggatacttt aagtgcaaaa tgttcgttta
    13321 ttttttgagg cactacccgt tgttgaacct cgggtagtgc ctctttttta ttgaaaggaa
    13381 tcaataagat ttgattcctt tatttatcaa taggttataa ttcatatttt tttatttttt
    13441 cttttacgat acttagtcca gaagctaaaa tttcaaggtt attagaggat agggaaattc
    13501 gaacgaatgc atctggttgc gcacgtgtag taaagcgtct ggaatgataa agtcggatac
    13561 cgttagcaag aaagtcattc tcaattatct tagggtcaat ctcaggatca actggaagaa
    13621 ttcgatagta agggacacga ttcgttggtt tttctacttt gaaaagagaa tcaaaaagct
    13681 gattggctct ttttgtaaga cttaatttag tttctaagat cctatttgct tcatttgaat
    13741 ggaggatttg agtgatgact tccacatcta atccagaagt tttcacattt atattaaaaa
    13801 ttgcttgttc tatcttgctt tggacatgta ctggaactac aagataagca actcgcaatc
    13861 cagaacaaat aaattttgtc attccagcta agtagattgt ttggttaggt aataaatttt
    13921 ggaaaggagg aatgatcgta tgttgatagt aagttgttaa aaatgaatgg atatcatctt
    13981 caataaccca tagaccttct ttttcaatca cttcggctaa ttgctcccgt cttttgtgag
    14041 agatttgaaa ccctatagga ttattacaag aaggcataag aaagacacca tgaatcttct
    14101 tttttctaca ttcctgaatc aatatatcag gtcgcattcc ttgctcgtca aaatcaattg
    14161 gaacgatctc taaatgatat aactgagcca attcaatgaa attagcgtat gtatatcggt
    14221 ctgttgctat tctatcacct ggagagaaaa gtgcagaaag tgtgatagcc aatccatttt
    14281 gaacaccgga aacaatagat agagattcta aggacggaat gactccttga gtttttaacc
    14341 aatttgctgc agtagtcaat tgaaaatttg tgcctttggg atcttggtaa ttaagcaaac
    14401 caacaagatt tgggttatta ctagttgatt gaataaacgg gacaacgaat ttattacatt
    14461 cttcaaaaga actaaccaga ccaaaatcaa tgatatgttc ggttacttca ttggtagaga
    14521 tggtgatgga ctcaaagaca ttgggagaaa taaatgtgcc acttccaata ttcgtataca
    14581 gcaaaccttt ctcaattcca tatttataag ccttgccaac ggtagtaaaa tttaaatcta
    14641 aataatcagc tagctctctt tgggagggta actgagtgtt tttctgtaat ttattcttta
    14701 aaatatcttg ttctaattgt tgaatcaacg atcgataata aggacgcttc aatttttgtt
    14761 tgtctggtcg ccaagttaac gggtaattgt cataagaatt aataggcatt ttctccactc
    14821 cttaaaattg tataccatac aattttacca ttgattgtat tgattttaga agttaatctt
    14881 ttgtgaggag ggaattgtct atgaaaatta ttaattttga ggaagtagta gcttctttta
    14941 gttttgaaga agcgattcaa gtaatgaaaa aatgtttttt tgattatgaa agtggtaaaa
    15001 tcagccaaaa tcctcgattg gtggaaattt tacctgacgg aaaaggcaaa aatatgtttg
    15061 cgatgatgcc tgcttatttg ggggagaaca gatattttgg cacaaaagtc attaccgctt
    15121 ttccggataa tcatcagtat catttacctt ctcatttagg agagattttc ttgtttgatg
    15181 ctcaaaacgg actacccaaa gcattgataa acgcaaatgc agtcacctgg attcgaacag
    15241 ctgctgtatc tgcgctggca acagattact tagcaaatcc gcatgcagaa acacttgctt
    15301 taattggagc tggtcaacaa gcattgtcgc atttgactgc gatttctacc attcgtccca
    15361 tcactacagt gtttgtttac gatattttag aagaaagacg aaatactttt atcgctaaag
    15421 ctaaaaaaca gtatcctaaa ataacgttta taaatgcaat atcagtagaa gatgcagtac
    15481 gtccagcgga aatcatttgt acgttgacct ctagtaaaga ggcatttctt caaaaagagt
    15541 gggtaaggga agacgcacat atcaatgctg tgggcacatt tactcctgat acaagagaga
    15601 taagtacatc gttgatggaa acttctcaaa tctatgtaga tgattactca gctgccttaa
    15661 cagaaagcgg ggacctgctt atagccatgg cagaaggaga tttagaggaa tcttgtattg
    15721 ttggtacatt aggcgaatta gtttctgaga aaaaaacatt ttataaagga gaatcgaggc
    15781 cgacgatttt cgacgcagtt ggtctagcta ttgaagatct ttgttgtgca gaatatattt
    15841 ataacaaatt agagaaaggg gctttaaaat gaacttagat tattacgtag ttgatgcatt
    15901 tgcagatgag gtgttcaaag gaaatccagc agcggtttat gtgctagaag aatggctgcc
    15961 agaaggtacg atgcaaaaaa ttgccattga aaacaattta tctgaaacag catttacagt
    16021 gaagaaaaac caagaatttg agcttagatg gtttacgcca gatcgagaaa tcgatctttg
    16081 tggtcacgcg acattagcaa cagcatttgt tttatttaac tattacaaaa tacctgatga
    16141 aacgattaaa ttttctactc aaagcggtaa tctatttgtg acaagaatta aagattatta
    16201 ttacatggat tttcctagta ttatgccgaa aaaagtgccg atattgtctg aatatgaaga
    16261 agcgattggg gctaagatta aagaagccta cttagcacgt gatttatttt tcgtattaga
    16321 agatgaaaaa acggtggcta aattgcagcc agattttaca gctatcaaaa attttgaatt
    16381 aggaatagga gtcatcgtga cagcagaaag tgtgcaaaaa gactttgttt ctagaacctt
    16441 ctttccaaaa ttaacaatca atgaagatcc agtatgtgga tcagcacatt ctaatcttat
    16501 tccttattgg gcagaaagaa tgaataaaac aaaattgtca gcttatcaaa tgtcatcaag
    16561 gggcggggat ctagaatgtg aattaaaaga agatcgtgtg ataataggag gaaaagcagt
    16621 tttgttttct aaaggtacct cgtttatttg atttctttca aatgaattaa aaataaattg
    16681 gtggagagtg gcaaccttat agctacaatg gttgttaaaa aaagacgcaa actttttcaa
    16741 tttaatgaaa agtttgcgtc tcattttaat tattggtgtt tgttttttat tcaatcaaga
    16801 gtaaaatgcc ccatactaac tatcctcgga tgacttcgat tttataacca tcagggtcaa
    16861 tgacaaagta gtaagaaggc gcagtgccag gtaatccttt taagtcagta atctcaagac
    16921 cagcagcttt atgttgctcg tgtaattttt ctagatcatc agctgctata gcgatatgac
    16981 catagccgtc tcctagatca tacgcaccat gatcatagtt gtaagtcaac tctaattcat
    17041 agtcatcccc aggaagtgtc aaataaacaa gtgtaaattt gttttctgga aaatcacggc
    17101 ggcggctttc ttcaaaacca aatgcctttt gataaaattc gattgatgct tcaagatctt
    17161 tcactcgaac acatgtgtga gccattttca tagattcatc tctccttttc cttttactca
    17221 atcataacaa aaagaagtaa ggaaatagtt gatttgtgct tgttcatgag aaatttctca
    17281 tgtaaaatgg atagaaaata aaactgttaa ggtggaaact attcatggat aaaccaattt
    17341 ttggtgaaaa aaagcaattt gctgattatc aaacccgtta tgcagcatat attgtcatag
    17401 aaagagatca gcagattgca gtaatcgagg ctccaaatgg tgcttttttt cttcctggcg
    17461 gggaaattga aggagaagag acgaaagaag aagcgattgt ccgtgagctg atagaagaaa
    17521 tgggaattgt ggcagaaatt tcttcttatc tgggagaagc tgatgagtat ttttactcga
    17581 attatcgtgc aacttattat tataatccag gatatttctt tgttgctgat tcttggaagc
    17641 gtataggaga accaactgaa aagaccaata aaatttggtg ggtgagccct caagaagcgc
    17701 ttgtgaaatt gaaacgtggt agccaccgat gggcagtcga gagatggatc gaaaaaaata
    17761 aaaactgaaa gaaagcgagt agatcagctt tctctcagtt tttttattta ttacagtaca
    17821 tattttttga ttgcttcagc tacaccgtgc tcgtcgtttg atgaagtgat gacatttgct
    17881 gcttctttga caagagaaac ggcatttccc attgctactc ctagaccagc atattcaatc
    17941 atggataagt cgttctcgtt atctccgata gccatgattt catcttgatc aataccaagg
    18001 tgctgtgcga gattcgcaac tgccgcacct ttacttgctt ctttatttag tacttcgaag
    18061 taatagggcg cacttttgac cgttgtgtat ttatcacgga aagtttgcgg taaacgcgca
    18121 attgctgcat ctaaaatttc tggttcgtcg atcatcatca ttttgacgat gttcatgtct
    18181 ggtgtcattt cttcaggtgt tcgatatttt agcggcatct tgactaaact tgcttcgtgg
    18241 atcgtataag cgctgatatc tcgattaggc gtgtagatcg ctttgtcatc gatcgaatgg
    18301 agatgactgc cgacacggcg tgccattact tcgatctcta ggaaatcttc atgtgtcagt
    18361 ccgtgacggg cgatgatttt acctgatttc gtttgttgta cgagtgcccc gttataagta
    18421 ataacgtaat cattatcttg aaaaaggttc aactcgttta actgttcctg gacacctggt
    18481 aagggacggc cagtgcataa aacgatcgaa acaccttttg caacagcagc ggcaatcgct
    18541 tcttttactt cttttgttac ttctcttttc tcatttaata aagtaccatc gatgtcgata
    18601 gcgactagtt tgatagacat aatttcctcc aatatatttt aatttatttc aatcaatgcg
    18661 ccattacgga tatggctggc aaattcttga taagtttcgt caaataaatc ataatgatca
    18721 cgcaaggacg gatcaaccat ttcttttgga aagaaaaatc gctcatctcc ttgttcttgt
    18781 cccgctaatg cagcaacaag ctgactaacc tgtgataatt ctatcaaagt tccgtcattt
    18841 cgcaaaatct cgatttgtgt gcgatgtctg ccttgttctg ggcgataaaa atcataaggt
    18901 agatcgtagc tagaattcac agcagtgtaa taaacaggat tatatccgac cttttctact
    18961 aacaaagtga gttcttggac taattttgaa tcccggttgc cagaaaaagt agcagatttc
    19021 agtggtttac gattcaaaaa acgtttagct aaatcattta aaataggatc agcagattca
    19081 gaccattgag taaagtaagt gcccaagacc ccatcatcaa gtttgaggta ttcttctaaa
    19141 gtaaagcttc cgtcaagaaa tggcagaagt aattgagaat ggagttcaaa actgctggca
    19201 tcttcttgat acaactcgtg tgcgcgatga agcagatgct ccaaaattac ttccatacca
    19261 cgggaaacag gatggaagta cacttgcaca tacatttgat atcggctaac gatataatct
    19321 tccactgcat gcatgccact catggaaaaa gcgatcccgc cttcataagg acgaatcaca
    19381 cgtaatattc gggtaagatc aaatgttcca tattctgttc cagtgaaata agcatcccga
    19441 agcaaatagt ccattcgatc tgcatcgatc tgactagaaa tcatttggac aacttgtggg
    19501 ttggggtaag ttttttggat cacactagct accttttcag gaaaaccttc ttctactcgg
    19561 ttaagaatct gatacacttc tgtttctgga gaagtgatga tctctacagt gatggcttca
    19621 tgattcgtat ggaagatatg ttcaaatgta tgagagtaag gaccatgacc gacatcatgc
    19681 aacagtgccg cacataaagt caccagccgt tcctgatcat cccagcctcc gttgcctatt
    19741 ttttcttttg aaaaatttcg agaaaagata tcgcagattc gtcttgaaat ctcataaacg
    19801 cctaatgaat gagtaaaacg gctatgttct gctccgtgaa aagtgaaaga agaagtgcct
    19861 aattgtttaa tgcgtcgtag acgttggact tctttcgaat tgatcaaatc taagattact
    19921 tgatgctgga catggatata gttatggact ggatcacgaa aaactttttc cataggcagc
    19981 atttgatatt tataaggggt tgtcataatg tagctccttt atgttattac gttgttccat
    20041 tctctggatt tggttgttcc attgctccag ctggatttct ttttttagcc agtcggtaga
    20101 atcctgtaaa tcacttgcag aatacagctt ttcaaaagct tttgcaaatg cttctttgac
    20161 ttcttttaca aaaaggggtt ggtcaagcag ttcttcaagt gttgccattg tagcaggatc
    20221 cactgcagga tagccatttg taccaaagtc ttcttttaaa gcagattgat aaaatcgctt
    20281 catgacttgc ccacgttttt cctgattacc gttgacactt aagtacatca taacagaaat
    20341 cccatttttg atccgacgct gagcgatacc ggcaaatttt ttgccatgga tgcttaaatc
    20401 ataagttcca ggacaataag aatgggtgat ttcgaatgct tgtatcggtg cctttggaaa
    20461 agcgtattta gtcaatagga gcatttgctc gtaagcgtca tctatggtta ttttttttgt
    20521 gtcactttgg gggaaaatca aagaaatatt caaaattcct tcatctgata cgatccctaa
    20581 tccacctgaa tttctgatca ctggagcgta acctgcatct tccagtgttt ggatcccgtc
    20641 acttagaaaa ggagttcgaa catccttcat acctaagatg actgtgcagt catattgcca
    20701 gaaatgtaaa aagatttgct ggtgttcttt agaatattcc gttaatatat ctgttaatgc
    20761 aaaaggggaa aagtcgtttt tttgataaat gtgctgatct agtcgaatcg cagggttcat
    20821 tcaaccggct cctttgtata aattcatatt ttctacaata ttatactcta aaatcaaaga
    20881 gttgttttgg aaaaaaggtt ttctgtaaaa atggacaagt tggttgacaa aacaaaagaa
    20941 actaggaaca atgtagctat gagaagtaaa ggagaatgaa taatggaatt atcacaagga
    21001 acaccgattt caatcaaatt gcgtacaaaa gtaaaacagc aaggggaagt gcaagatttt
    21061 tattttgatt tgaaagggca aatggtgaca attggcgata cgctgtacat acgttataaa
    21121 gagattcagg aggagacggg cgatgagatt cctgtaacga tcaaattagt tccagatggt
    21181 catgtacagc tgatccgtgc aggagaaatg cggatgcgtt tgaaatttgg ctataaagaa
    21241 cggttagaaa catcctatcg aacaccatac ggtatgatcc agattgctac ttttacgaaa
    21301 gaactgcatg ttagtttgaa agaccgtccc acagcaggga aggttcgaat cgattatgat
    21361 ctgttcatgg gaccggaaaa aatcggcgaa tattatttga ccttggattt tacagcgtaa
    21421 atataaaaga aaagtttgat tttttgtgtc tgattttgta tgataaggag tagactgtga
    21481 aaggacgtgt cctgattgga aattagtgta tttgaaggtg caaacaaaag cgaattatca
    21541 atgatcgaag tagcacacgc gatcttagaa caacgtggcg atgtcatgga tttttctgat
    21601 ttagctaacc agatccaaaa ctatcttggg aaatcagaca gtgaaattcg tgattcactc
    21661 gcacaatttt atactgactt gaatattgac ggcagcttta tctcattagg ggataatcgt
    21721 tggggcttgc gttcatggta tgctatcgat tcgatcgatg aagaagtaaa ccatggaatg
    21781 gacgaagagg atgaagatac tccacgtcgc cgtaaacgca agaaagtcaa cgcctttatc
    21841 aatgatgatg aagacgcgat cgattacaat gacgatgacc cagaagatac agatctgaca
    21901 gaagaagatg acgatgatct gtttgatgac gatgatgatg aagatgaaga aatcgctgct
    21961 tataattctg acttgcaaga aatcggagca gatgacccga cagatgatga agaagacttg
    22021 ccaggaaata tcgaagaaga tttaagtatc atagacgaag acgatgatga tgaagattat
    22081 gaagaggaag aattttctga ctctgaaaaa gaataaacat tattttctta cgagaggttg
    22141 tgacagagac gatcagccta tcagaataag gaggtgtgac acgatttttg tcacaccttc
    22201 ttttttgtat ataaaaatcc caagtaaata cataaaaaat aaaaaaattg aaacttttag
    22261 cgcaccttgt acgtcatatt aagtgaggca gaaaggagtg gagaatgatt tttactggat
    22321 ataagaaaaa acggcagatc gaaaaatacc gtcacctgct gaatggactc atcgaaactg
    22381 attatcaaaa aatgttccgt atcacgatga attacgtaca tgataaagaa gaggccttag
    22441 atgtgatgca agatagtttt cataaagccc tgaactattt tgaaaaagaa aaagagatcg
    22501 agcattttag tgcatggttt tatcggatat tgatccgcac ggcattagat agttggcgca
    22561 gacaaaaaag gacgcctgtg gacttatttg aaattcaaga tatcccccaa cagttacctt
    22621 tagaaacttc cgttgcagag ctccatatga ttttagataa aatagacagt ccggaaaaag
    22681 agattctgat cctctacttt tttgaaggat ttcgtttgaa agaaattgcg actatcttgg
    22741 atatgaatga aaacacagtt aaaacgaaaa tgtaccgctc attgaatgct ttacgtcaaa
    22801 tcctagaatg aaggagagaa gagtgccatg tattcccaaa aagaaaattt aaaaaaacta
    22861 aagaaaactt ataaagaaca agcaattccc aaagggctta aagaggagat ccgccagcgt
    22921 tttattatcg aagagcaatc atttataaaa gcaaagcgga gaaagagacg tctccaaggg
    22981 ttcggagtat ctttggctat cgtaatgatt acaacaagta gccttctttt taatagtcaa
    23041 gttcgaagct ttgcagaaga tttacccatc ttaggatcag tcattgaatt gatcttaggc
    23101 gaacgcttta ctgatcgatc tgaaaaaatc gatattcagg taccgaagat caatacacaa
    23161 aatgagaaag aaaataaaac aattcatgga ttaaatcaaa aatactttcg cgaaggacaa
    23221 gcagattttg aaaaagctga aaaagaatat ggaaattttg aaacggatca ttaccaagtt
    23281 ttgggagatt atcagaaaat cgtagacgat aaccggtttc tcgtgattga aaggcagatc
    23341 acccaaacag cagcagatag ccatgttgaa aaaagatacg acacaatcga caagaaaaac
    23401 agtgtccagc tctcacttcc acttttattc aaagatgaca cctatctatc tgttttgaca
    23461 aaagaagtca aacggcagat ggccgaacaa gtcaaggaag atccatctaa gtactattgg
    23521 actgagcaag atattcaaga agggacgata gaaaaaccaa cacttgttac gcctacacga
    23581 agtttttatt tgaacaaaga gcatcaatta gttttgactt tttctcaata cgaaattgct
    23641 cctggttata tgggtacacc agaatttgtc attcctaagt ccgtgacaaa aacgattttg
    23701 gcatcggaag attatttgga tcattagacg agaagtagtt gcgactagca gtttcaattt
    23761 tcaagtaagt acaagtaaat aataagaaga gaaaaagaga aagaagagat atagtgaaaa
    23821 gaaattattt agttatcagt attattgctt tatccgtgct tttgtttttc gaaattactt
    23881 cggtggcctc cttgatcacc gaagtaaaag gacaaacaca ctcttcggag cattcgtcac
    23941 aatctttcaa acaatcagaa caatcaacca ttaaaagcca atcaactgaa acagctaacg
    24001 tcaaagaagc cattcaagaa tcagcggaaa aggtacaaag tcagtcatca caatcaacac
    24061 aagacagtac aaaaacgagc cgagatccgg ctgtcaaaag cgtcgcaatc agctttgatg
    24121 atggaccagg agcgaccaca acaccgcagc ttctgcgaat cttaaaagaa aaaaacgtgc
    24181 atgccacttt tttcgtctta ggagaaaata cggcgcagca tcctgaaatc gtgaagcaga
    24241 cagctgaagc agggcacgag attggcaatc acacatacga tcatcagaat ctggccactc
    24301 tttctgccca gtcgatgacg gaggaagtga cgaaagcaga cactgagatc aaaaaagtca
    24361 ctggcaaaac ccccactttt gttcgtccgc cctatggttc tgtcacgaat gtaggagcaa
    24421 cgatcatcca gcgaccgatc atcgaatggt ctgttgattc agaagattgg aagacaagaa
    24481 atcctgattt gattttgcag aagatccaag caacagtata cgatggggca attattttat
    24541 tccatgatat ctatcctgaa acgattcgtg ccgttcctca agtgatcgat tatctgaaag
    24601 agcaagggta tcgcatcacg acagttgggg atttattagg gcatccaacg gctgtcgaga
    24661 actattatgg cagaaacgat catcggccag ttcagtaagt gagacaagta tctttaataa
    24721 ataacacatt ttaataagta gttgaacaca taactacctt acttgcaaag gcttaacaat
    24781 atctatacag gtacaacaaa aaactgtcca ccaattttct ttggcggaca gttttcactt
    24841 aaaaataaag aagcaactat tcgtaaatta tgctaaaata gcagtagaac gcgtcctgag
    24901 ggaatcgaac ccccgtctca agaaccggaa tcttacgtga tatccactac actaaggacg
    24961 caagcactgg gataattgta actaaacaga taaaaaatta caaggggatt ggacaaaaaa
    25021 atgaaatttg agcaaaattt ttcacaaaaa cagcagcaga cacagaaact agccatgacg
    25081 caacagttgc aacagtccat ccaagtttta caatataaca gtgaggagct gttggcattc
    25141 gttgagaatc aagcaatgga aaatccattg gtggaagttg ttgagccgga atggcagcca
    25201 gattacataa aagcttcttc atcttcttat gaaggagaag agacaaatta cctaaatcag
    25261 attcctgata caaaagggtc tttatttgat tcattgatcg aacaagtcca tttgaattac
    25321 cgagatactt ttttgaggaa aatcgttctt tatcttgtag aatacatcga tttgaacgga
    25381 tttttgacga ttagtttaga agaagccgga aaagagattg gagctacgcc tatccaaatg
    25441 ctcgatgcgt tgactctgat tcagcaatta gaaccagctg gagtaggtgc acgtagtttg
    25501 caagaatgtc tcatgcttca aacagaacga gatgattatg cgccggaatt agcttatatc
    25561 gtgttggaag aatgttttga ggaactggtg gagcgaaaat ggaaagaaat cgcacaacgt
    25621 ttttctgttg atctacatgc tgttcagcag atattcgact atttacaaac gttgagtcca
    25681 agccctggcc ggattttcga ccgtagcagc gaattgtaca ttattcctga tgtccgggta
    25741 ttagtggacg acgataaaaa tgtacaagtt atctcaaacc gaaaaaatca gccaaatata
    25801 cgctttcaag agagttactt caaacaaatg tcacaacaag cagataaaga aactgaaaat
    25861 tatttaaaag aacggaaaca ggaattcgaa tggttgaaga aaacgatcct acaacgtggg
    25921 gatacaattt tgcgggttgc tcaagtcatt gtttcccggc aaaaagcttt ctttatcgac
    25981 aaggaacgtc caatcaagcc tcttacgtta aaggaagttg caactgagat agatgtgcat
    26041 gaatccacgg tcagtcgtgc agtcaacggg aaatatttgg aaacagactt cggtgtattt
    26101 gaattaaaaa aatttttcac tacacgaatt cctacgaata gtacagaaca gacagaagac
    26161 ctttcagcag atacagcgaa gaaaaagctg caggaattag tcgatcaaga agacaaaaac
    26221 aagccgttgt ctgaccagaa attggttgaa ttgctgaaaa aagatgagat agcgatctca
    26281 agacgaacag tagcaaaata tcgtgattta ttaggaatcc cgagttcatc aaaaagaaaa
    26341 cgttatgata actaatcagt caaaagacta gaaaaagtat ttcacttttt ctagtctttt
    26401 tagttgaaaa acgagagcaa aactgttaga cttacagtcg tgaggttgag atttcccttt
    26461 attgatgtga aattcgaata gttaatttct ttttttatcg tatatgggtc gttttaagtc
    26521 tacattggac gtaaagtgtc caactaagga gatcgctatg ctggatgaaa taaaaatgat
    26581 tgaatctgtt gcaccagata ttatcgaagt gttgcaagaa cgctataaaa ttttacgaaa
    26641 tatctattgg atgcagccga ttggacgtcg aagtttatcg gaaagtatgg ggatcacaga
    26701 acgagtattg cgaacagaaa cagatctttt gaaaaatctg aatttgatcg acacctcaaa
    26761 aagcggtatg acgctgacaa ctaagggcga agaagtttat caaagccttg aaaacttcat
    26821 ggatcagctt ttaggtacgc atcaaaccga acaacagttg gcagaatatt ttggtaacca
    26881 acgctgtatc gttgtttcag gaaacagcga agagcagacc aaagtggctg atgcattcgg
    26941 cgaagcgctg agcgaagccc tagaccgact acttccagaa ggcgagaata tcatcgctgt
    27001 gatgggagga actactatgg ctgtggtagc cgaacaattg tctaatctgg aaaataagaa
    27061 acggcataac ctgtttgtcc ctgcaagagg cgggattggt gaagcgataa ctgtacaggc
    27121 aaattctgtc agtgcaagaa tggctgctaa agcaaacggt aaccaccgtg cactttatgt
    27181 tccggaacaa ttaagtctgg caacttacaa ttctttactc aatgagccat ctatccaaga
    27241 agtcttaaac ttgattagtg aagcgaattg tgttattcat agtattgggc gtgcattaca
    27301 catggcagca cgtcgtaaga tgactgaaaa agaattagtc atgctgaaac aagcaaatgc
    27361 tgttgcggaa tcttttggct actttttcaa tgaaaaaggt gaagtcgttt ataaggtccc
    27421 tagaatcggc atagaattgc aagatttgga aaaagtcccg atcatcatgg caatcgcggg
    27481 cggaaaatca aaagccaaag caattcgagc ttatatgaaa aatgcaccaa aacaaacgtg
    27541 gctcatcact gatgaagctg ctgcaaatga gattttaaaa ggggtaaccc tttaaaataa
    27601 atattttttt gattttcata aggaggaaat cttaaatgac agttaaagta ggtattaatg
    27661 gttttggacg tattggacgt ctagcattcc gtcgtatcca agatgtagat ggaatcgaag
    27721 tagtagcaat caacgacttg acagatgcta aaatgttggc acacttgtta aaatacgaca
    27781 caactcaagg acgtttcaac ggaacagttg aagttcatga aggttcattc aacgtaaacg
    27841 gaaaagaagt taaagtatta gctaaccgca acccagaaga attaccatgg ggcgaactag
    27901 gcgtagatat cgttctagaa tgtactggtt tcttcacttc taaatcagca gctgaaaaac
    27961 atttaacagc tggtgctaaa cgcgttgtta tctctgctcc tggcggaaac gatgttccaa
    28021 caatcgttta taacacaaac cacgaaacat taactggtaa agaaacagtt atctcaggtg
    28081 cttcatgtac tactaactgt ttagctccta tggctaaaac attgaacgac aaatttggtg
    28141 ttgttgaagg attaatgaca actatccacg cttacacagg tgaccaaatg actctagacg
    28201 gacctcatcc taaaggtgac ttccgccgtg cacgcgctgc tgctgcaaac atcgttccta
    28261 actcaacagg tgctgctaaa gctatcggtt tagtaattcc tgaattgaac ggtaaattag
    28321 acggagctgc tcaacgtgtt cctgtaccaa caggttcatt aacagaatta gtaacagttc
    28381 ttgaaaaaga agtaactgtt gacgaaatca atgcagcaat gaaagaagct tcaaacgaat
    28441 cttacggata caacacagac gaaatcgttt cttcagatat cgttggtatg acttacggtt
    28501 cattatttga tgctacacaa actaaagtaa tgacagttgg cgacaaacaa ttagttaaaa
    28561 ctgttgcttg gtacgacaac gaaatgtcat acactgcaca attagtacgt actttagaat
    28621 acttcgctaa cttataagat agacaaacgt acaagtgtaa attatagaca tgtacattta
    28681 aaagcgggga agcaatcgcg cttcttccgc tttttttgat aataagttta ttcaggagga
    28741 aataaaatgg ctaagaaaac agtgaaagat atcgatttaa aagacaaaaa agttcttgtc
    28801 cgtgttgact tcaacgttcc tttgaaagac ggcgtgatca ctgacgacac tcgtatcaaa
    28861 gcagcattgc caacaatcaa ctacgttcta gaacaaggcg gaaaagcaat ccttttctct
    28921 caccttggac gtgtaaaaac agaagaagat aaagaaggaa aatctttagc tccagttgca
    28981 aaacgtctag gcgaattatt aggtaaagaa gtaacattcg tacctgaaac tcgcggagaa
    29041 caattagaag aagcaatccg caacatgaat gacggcgacg ttgtcgtatt cgaaaataca
    29101 cgatttgaag atattgacgg taaaaaagaa agcaaaaatg atacagaact aggtaaatac
    29161 tgggcttcat taggcgatgt atttgttaac gatgctttcg gtacagctca ccgtgcacat
    29221 gcttctaacg taggtatcgc ttcaactggt atcccaacag ttgctggatt cttgatggaa
    29281 aaagaaatca agttcatcgg tgaagcagta gaagaaccaa aacgaccaat gattgcaatc
    29341 cttggtggcg caaaagtttc tgataaaatc ggcgtaatcg aaaacttgat tcctaaagca
    29401 gataaaatct tgatcggtgg cggaatgact tacacattct acgaagcaaa aggaatcaag
    29461 atcggtaact ctctagtaga agctgataaa gtggaactag caaaagaatt aatcgaaaaa
    29521 gcaggcgaca aacttgtctt gccaatcgac aatgtctgtg caccagaatt ttcaaatgat
    29581 gttgaaacac aagtaatcga aggagacatt cctgatggtt tgatggcttt agacatcggc
    29641 ccagcatcag taaaattatt tgctgataca ttacaaggtg caaaaacagt tgtttggaac
    29701 ggacctatgg gtgtatttga aatgagtaac tttgctaaag gtacaatcgg tgtttgtgaa
    29761 gcaatcgcta acttagaagg cgcaacaaca atcatcggtg gcggagactc agccgcagca
    29821 gcagaacaat taggatttgc tgacaaattc acacacatct caacaggtgg cggcgcaagc
    29881 ttggaattgc ttgaaggtaa agaattacca ggactagcag caatcaacga taaataaacg
    29941 accaagtttg ctcaattctg acgagtaatc gatcaaaagg tgaagggagt tttcatgact
    30001 ccctagccat ttttcaataa attaaggagt gtgttcctca atgcgtaaac ctattatcgc
    30061 aggtaactgg aaaatgaaca aaactgcatc agaagcaaaa gaatttgctg aagcagtaaa
    30121 aaacaaaatc ccttcaaatg acaaagttga ttcagtcatc ggatctcctg cattattctt
    30181 acaagaatta atgacatcag ctgaaggtac agaactaaaa atctcagcac aaaactgcta
    30241 ctgggaaaac tcaggtgcgt tcactggtga aacttcacca gcagctcttg ctgatctagg
    30301 tgttcattat gtgatcatcg gacattcaga acgtcgtgaa tatttccatg aaacagacga
    30361 agacatcaat aaaaaagcga aagctatctt tgcaaatggt atgacaccaa tcctatgctg
    30421 tggcgaatct ttagaaacct atgaagcagg caaaactgct gaatggatcg aaggacaaat
    30481 tacagctggt ttagctgatc tttcagctga acaagtgtca aacatggtta ttgcttatga
    30541 accaatctgg gcaatcggta ctggtaaatc agctgacgca caaatcgctg acgaaatttg
    30601 tggagttgta cgtcaaacag ttgaaaaact ttatggaaaa gaagtatcag aagcagttcg
    30661 tatccaatac ggcggttctg taaaaccaga aaatattgct gaatacatgg caaaagaaaa
    30721 tgttgacggt gctttagtcg gtggcgcaag cttgcaagca gattcattct tgtcattgtt
    30781 agacgctgta aaataattgt tgaaaatttg cgaaaaatgc aagaaatgtt tgcactaatg
    30841 agcaaaatta actaaaatcg tatatgaggg aaatccctta ggaaaaaaca aactcaaagg
    30901 agagacaaaa catgtcaatt attactgatg tttacgctcg cgagatctta gactcacgtg
    30961 gtaacccaac aatcgaagta gaagtataca cagaaagcgg agcttttggc cgcggaatgg
    31021 ttccttcagg tgcttcaact ggtgaatacg aagcagttga attacgtgac ggagacaaag
    31081 ctcgttatgg cggtaaaggt gtaactaaag ctgtcgacaa cgtaaacaac atcattgctg
    31141 aagcaatcat tggctacgat gttcgtgacc aaatggctat cgacaaagct atgatcgctt
    31201 tagacggaac tcctaacaaa ggtaaattag gtgcgaacgc tattttaggt gtttctatcg
    31261 ctgttgcacg tgcagctgct gactacctag aagtaccttt ataccactac ctaggcggat
    31321 tcaacacaaa agtattgcca acaccaatga tgaacatcat caacggcgga tcacatgctg
    31381 ataactcaat cgacttccaa gaattcatga tcatgcctgt tggcgctcca acattcaaag
    31441 aagctctacg tatgggtgct gaagtattcc acgcattagc ttcaatcttg aaagctcgcg
    31501 gattagcaac ttctgttggt gacgaaggtg gatttgctcc aaaccttggt tcaaacgaag
    31561 aaggttttga agtaatcatc gaagcaatcg aaaaagctgg ctatgtacct ggtaaagacg
    31621 ttgttcttgc tatggatgct gcttcttcag aattctacga caaagaaaaa ggtgtttacg
    31681 tactagctga ttcaggcgaa ggcgaaaaaa caactgacga aatgatcaaa ttctacgaag
    31741 aattagtttc taaatatcca atcatctcaa tcgaagacgg attagacgaa aacgactggg
    31801 acggattcaa gaaattaact gacgtattag gcgacaaagt tcaattagtt ggtgacgact
    31861 tgttcgtaac aaacactcaa aaattatctg aaggtatcga aaaaggaatt gctaactcaa
    31921 tccttatcaa agtgaaccaa atcggtacat taacagaaac tttcgaagct atcgaaatgg
    31981 ctaaagaagc tggctacaca gcagttgtat ctcaccgttc tggtgaaaca gaagattcaa
    32041 caatctctga tatcgctgtt gcaacaaacg ctggccaaat caaaactggt tctctatcac
    32101 gtactgaccg tattgctaaa tacaaccaat tactacgtat cgaagaccaa cttggtgaag
    32161 ttgcagaata caaaggtttg aaatctttct acaacttaaa aaacaaataa ttcttttcta
    32221 atgaagatac atgatcgact attgaaagat agtcgagtat gatcacttag ttaagtaatc
    32281 gaactctcta cttgctgaaa tatcagcagg tagagagttt tttttgcaac tagcttctgt
    32341 atatgatgtt tacaaagaat ggtacaagaa aagatcatcc ttgaaaaagg aaagctaaca
    32401 ataactagca tcatataaga gcttttaagg atagaacagt agaataggaa tctatgagca
    32461 agtcctctcg tataaataaa ataggaggga agtagcagca tatttttcat aaagtggctc
    32521 tctttcttca tttttacttt ggttgctcct atttgaaaat tcgagcacac tcaaatacaa
    32581 taaaaagaaa tggcaaggga gaggattaga tgaaaaaaga agaaaaaatc gcgattttac
    32641 aagagatcat ccgaattaaa tcagtgaatg gaaacgaagg tgaagtagca gcctatttga
    32701 ataagctttt ggcaaagcat gacataacag gagagattgt ttcttacaga gatggtagag
    32761 ataatttgat tgctcgttac caaaagggcc aatcaggaaa agtgttaggc ctatctgggc
    32821 atatggatgt cgtggcggct ggtgatgaat cgtcttggac ctatgcaccc tttgcagcag
    32881 aaatccatgg gaaccgtttg tacggacgtg gtgctacaga tatgaagagt ggcttggcag
    32941 caatggtgat tgccatgatc gaactgaaag agtcaggaaa accatttaac ggaacagtca
    33001 aattattagc cactgtcgga gaagaagtgg gtgaactagg tggggaacag ctcacgaaag
    33061 caggctatgt agatgactta gatgccttga tcatcggaga gccaactaat tacagtttga
    33121 tgtatacgca catgggatca atcaactata cagtcacctc gcacggaaaa gaagcgcata
    33181 gctctatgcc agaccaaggt tataatgcaa tcaatcacct aaatgaattc attacgaaag
    33241 caaatgctga aatgaatcac ctcgctgaaa caatcgagaa tcctgtccta ggaaaaacaa
    33301 tccataatgt tacattgatc agtggcggga atcaagtcaa cagcatccca agccatgcgc
    33361 agctccaagg gaacattcga tcgattccag aatacccaaa tgataaaatc attgcattgc
    33421 tgcaatcaat cgtcaatgaa ttgaatcaag agacagacta tcatttggaa ttgatgatcg
    33481 attacaacaa aatacctgtc aaagctgatc cagattctcc tttgattcat tgcattcagc
    33541 aacaattcag ccagccatta cctttagttg gagcggcagc aacaacggat gcagccgaat
    33601 ttacgaaagc taaccattcc ttcgactttg tcgtctttgg gcctggtgtc gttactttgc
    33661 ctcatcaagt agatgagtat gttgaaatcg ataattattt ggatatgatt gaaaaatatc
    33721 aaggaattat tttatcctac ttagcgtaag atttttttaa taaatgaaaa aacagagcca
    33781 gattagcatt attttgctaa tccagctctg tttattcagc ctatttttct ttttgaatat
    33841 atcaagcgtt gtggcaacat agtgatgact aatgaaacaa cgacgataga caacaaacga
    33901 tcaaggtagt ctgtacctgc ctgtaccaag atcacacttg ttgtctgact taaacctaac
    33961 ccatgtaata gctgaacgat catactggat cctgaggagg tgatcccgcc aaataactga
    34021 acggtaatga tcgaagaaac gatcgttcca ggtaccgaca aaatcaaagc gagtaataac
    34081 agtttcaagc tgtttttatc tttgccgaga tatcttggca aaatcaatcc agcaagcaag
    34141 cctgtgagaa tttggattgg cgaataatac agagaaaaaa tatctgtagt cactccactt
    34201 aaaataccgc tggacaatcc tgtcagcatt ccaggtacag gcccaagcaa tgcaccagca
    34261 aaaatcgttc cgatagaatc gagatagatt ggcaaacgaa gccataaagc aatcgttcct
    34321 ccaatataat tgatcccaat acacaaagcc atcactgtaa gtacgcgagt tgccattttg
    34381 tttttattca ttttgttagc ccatctttaa cctttccaaa tctttcataa tcgtttcttc
    34441 ttgtgcattt aaaagaacgg ttaaaaactt tcggaaaaaa agacgagtat ctacttttgt
    34501 cataatttta ctgttagcag gtttctgcca aaattcataa cgatcaacca atgtttgtcc
    34561 acgacttatt ccttgagttt ccacagcagt gaagcttttg aaaccttcac agatggcccc
    34621 ctcgataaaa taagcaacag ctaacggatc attgatgaca catcctaaaa ttctttcttg
    34681 tttccagtgg aagtcaaaat aaaaacgagt gatcgctttt agatactctc cctcttcagg
    34741 attcatttga cagcagtatt cgaggatagt cggtgtcaag acgatttctc ttgtcacatc
    34801 taacccaacc atttctatcg tttgtttgag attctcgaat acataagaag ctgcttcagg
    34861 atcacaccag taattgtatt cagcaactgg cgaacagtta ccatggcttt tgtaagttcc
    34921 acccatcgag acaaaacgat ccatatgttt cccaatattt gggttgactt tcaaagcaaa
    34981 agcgatattg gtcaaaggac ccaaagcaat gactgatgta tttgtctttt ctttgaaata
    35041 atcagccaaa aaatcaacgg catggagagg ttctgcctgt tttctcaaga tcatcgggaa
    35101 atttgtttcc cctaaaccat ccataccatg agtgtcttgt gcactgatga aaggttgctt
    35161 caaaggtttt tccattcctt gataaacagg aatgtctaaa cgatcacagc gttccaggca
    35221 tttcagcaca ttctgcgtac ctatatctgc agggacattc ccacaaacta cggtaatagc
    35281 aactacttcg atatcagggg atttgacagc taatgataaa gctaaagtgt cgtcgatccc
    35341 tggatcgcaa tcaataatta cttttcgcat tatttccaat ctcctttctt tagagatact
    35401 tagtttttta ttctgggaag tttccgaacc agtccaagac gagcgtcttg attataatgc
    35461 acgaaattag tatactggct tttattgcga atgtaaatgc tctcttacaa gtaaatctat
    35521 tgaggagaac tttaagtaaa tgatacaatg aaaaagaaat ggaatttacg tgtgccgaaa
    35581 ataaagaatc ctatggatcg gtacattgga taatagttag attatagttt ggcagaaagc
    35641 ggcgggtgta caatggttat tttttatttc attcttattc tgataattat agggatcagt
    35701 taccattttc tttcttattt ttatgcaaag aaattagtaa gagcgggagt acagagagga
    35761 tatgactggt atgaagacac tggtcatcgt ttgatggacc ctgctgcagt cacgtcagta
    35821 gaaaaaaaac gacaaaaatt agaagaagag tgtgaaagct tttgggagca gggaacaccc
    35881 aaaacaatca aaagttatga cgggttgaaa ctagctggtc agatgttcat ccatccaaat
    35941 aagcagaata aatgggtcat ttgcgtacat gattaccgaa gcacagggaa aagagacatg
    36001 tctcatatag gtaaaagata tgcagagaaa ggattcaacg ttcttgtacc tgatttacgt
    36061 gcgcacggag aaagtgaagg agaaatcatc ggaatgggat ggctagatcg gctagattta
    36121 attgcctgga tccaactcat cttagatgaa cagccgaatg cgtccattat tttacacggc
    36181 ggatcaatgg gagcttctac gatcatgatg gcaagtggtg aaaaacttcc atcagcggtc
    36241 aaaggattta ttttagacag cggatatgtt tctgtttatg cagaattccg atatatgtta
    36301 agtaaaatca ctgtctttcc taaaaaaatg gtcatgagat atgcgaatca ttatgcccag
    36361 aaatacgccg gttactcctt aaagcaagca tcagctacga gacagcttgg cagcaaccat
    36421 ctgcctttat tggtcattca tggtgaaaga gatcattttg tccctacaga agcagcgtac
    36481 acgattcaaa atgctacggc aggggataaa gcactgctgc ttgttccaga agcagaacac
    36541 ttggaagcat cgatgaaaga tccgaaaacc tattggacag tgattttttc atttattgaa
    36601 caaagaataa cgatgaatta attttctctt cttttttgta agtaattcga ggataaataa
    36661 ttgtttatag atcaggagac tgctaaaata aggtaggaat aaaaaaatat cagttatttg
    36721 aggagtgcgg catatggaaa tcaaagaaga aaaaaatcgt cttgctttat tcaatgatga
    36781 acaacaagag atcggagaaa tgacatggtc agatgcgggc ccagacatca tgatcattga
    36841 ccatacattt gtagatccag catatcgtgg tcaaaagtta gctgaaaagc tagtgtatac
    36901 tggagtagaa ttagcaagac gagacgggaa aaagatcatt cctttatgcc cttatgcaaa
    36961 aaaagagttt gaaaagaaac cagaatatca agacgtctta cgtcagtcat aagaataaca
    37021 gctgaaagtt ttgaactttc agctgttatt ccatttatat agagttgtca cggactttta
    37081 tctatgataa agttaaagag acgatatctt ttgggaagga ggctgcgata tgtataattt
    37141 aattttggga atcgtgatcg ttttatcgat tgttatggtc attgcgatta tgatgcagcc
    37201 aagcaagcaa aatagtgcag cgagtgcatt taccggcggt gcggatcaac tattcggcaa
    37261 acaaaaagct cgcggctttg aagctgtcat gcagcgatca actgcagtta tgggagctat
    37321 ctggatgatc ttattatttg tcctagcata tttatcttcg aagtagttga ataagcctct
    37381 cgcaaaacgg cgggcggctt tttttattgc aaagagaacg cttaagaaga agttatgaaa
    37441 gaaactatgg taaaatgagg cgaaagaggt gagacgagtg agacaattac caaaacccct
    37501 ttatgcaaaa catggaaagc gagcggtttt gctgcttcac gcttactcag gaagtccaaa
    37561 cgatgttcga atgctcgcac gatttttgga aaaagcagat tatacagttt atgcgccgct
    37621 ttttaaagga cacggaacaa tggagccata tgatattttg caagaaaaag cagaaagctg
    37681 gtgggcagac actaaaaaag ccatccattt tttacagtct gagcagtttt ctgatattgc
    37741 cgtattagga ttatcaatgg gcggcatctt tgctgtacga gcactagaag aagaatcagt
    37801 gattggcggc ggttttttct gttcgccttt gtctcctgta aaaacaaacg tgcctgaaaa
    37861 ctttgaaaaa tacgtacgtc aagtgctgaa gacagcaggt aaatcagaga aagaaatcaa
    37921 tgaaaaagca gttgcttacc gctcgttagc tgaacaacaa ttaatggata tacaagatca
    37981 ggcagcaatc gtagaaagta ggttgtcaga tatccaacag ccgatatttt tagctcaagc
    38041 tgggaaagat gaaatgatcg accctaatgg cgtatttgaa actgcacgca agttgagccg
    38101 tcagcgagtg acacttcgat ggtatcctga aagtggtcat gtgattacag ttggtacagc
    38161 aagacgcgaa ttagaaaaag acgtcttgga atttttagaa aaattacctt ggaatgagga
    38221 ataaatatga caaaagaaac gataaaagag aaaattctct ttttcatgga aagcagtcgc
    38281 aaacgcagtt tttctatgga agaaatcgct gaaggtttag ggttccaaaa aagcgatgat
    38341 tttaaattac tagtacaaac gattgctcaa atggaaagag aacagagtat agttttcaat
    38401 aaaaaaggaa aagtgaaact tcctttaaaa ccaatcttga tcgaaggaac atttcgagca
    38461 aatgaacgag gatttggatt tgtaacgatt gatcccgaag aagatgacat ctatatccca
    38521 aaagaagcga ctggttttgc aatggatgga gacaccgtag caatcgatat catgaagcga
    38581 gcaaatacag atatggatcg cggagcagag gggaaagtag tcgagattcg agaccgggcg
    38641 actactcagt tagcagggga attcgttgct tattcagaag aagaaataca agaaacggat
    38701 ctttacggcg ttgttatccc gaaagataga aagctcaacc aatttaaagt atatgcagca
    38761 gcagagggag ttcgtccagt tgacggaagt attgtgctga tcgaactgac ccattaccct
    38821 gaaaaagggt atgcgactag tttagaaggg ataatcaagc aagtgattgg tcataagaat
    38881 gaccctggca tggatatcct ctcaattgtc gtggctaatg gtatccctac gaaatttcct
    38941 gatgatgtat tgtctgaagc agatgaagtt ccagatcaaa tcagcgaaaa ggatataact
    39001 ggacgtagag atttaagaaa tcagctcatt gttacgattg atggagaaga tgcaaaagat
    39061 ctagatgatg cagttacggt taaaaaatta gataacggta attattttct tggagtacac
    39121 attgcagatg tttcttacta tgtgacacaa ggcagtcaat tggacagaga agcatatgaa
    39181 agagggacaa gtgtatattt gactgatcga gtgattccga tgatcccgca acgtttatcg
    39241 aatggtatct gttcactgaa tccccatgtt ccaaggctga ccatgagttg cgaaatggag
    39301 attgatccaa atggacaagt agtctctcat gatattttcc caagtgtgat ccaaacaaca
    39361 gaacggatga cctacacagc tgtcaatcaa atcttagaag atcaagacga agaaacgatg
    39421 caacgctatg ctcagctcgt acctatgttc caagaaatgg gtgaattgca tcgaatcctt
    39481 gaaacgatgc gaatcaaacg tggcgcgatt tcttttgagg atcgcgaagc aaaaatcctt
    39541 gttgatgctg aaggacaccc aaatgatatc cagctaagaa gcagaggaat tggtgaacgc
    39601 ttgatcgaat ctttcatgct tgcggccaat gaaacggtag ctgaacatta taataaatta
    39661 aatctgccgt ttatttaccg aatccatgaa cagccaaaag aagaaaaaat gcagcgcttc
    39721 tttgattttg cttcagcttt gggcattctg gtccgaggaa cgaaaggaac gatcacacca
    39781 aaagatttac agcatgtgat cgaaggagta gaagacaaac cagaagctgc agttatcaat
    39841 actatgttgc taagaagcat gcaacaagca cgctattcag aagacaattt tgggcattat
    39901 ggcttagcgg cagattacta cacacacttt acatctccta ttcgacgcta tcccgatcta
    39961 atcgtccatc gtctgatccg ttcttacagt gaagataaaa gtgaaagcaa tcaaagttcg
    40021 tgggctacgg aacttcctga aattgcagat catagttcta agatggaacg tcgtgcagta
    40081 gaaacagaac gtgaagtaga ttccatgaag aaagcagaat tcatgatgga caaagtgggg
    40141 gaagaattcg atgggatcat cagctctgtt gtgaaattcg gcctgtttat cgaactgccg
    40201 aatacagtag agggattgat ccatatcaat gaattgaaac aggactactt ccattttgtt
    40261 gaaaatcatt tagctcttgt aggtgaaaga actggtctga cttttaaaat cggacagaaa
    40321 gtgcgagtaa aagtagtcaa agctgatcca gatgaacgag cagtagattt tgaacttgtt
    40381 tccgcagaag aggttacagc gttggaacgg ccaaaaacac gaaatggaaa atcaggaaaa
    40441 cgcgaaggaa aacaagcagg aaaacgccca gaaagaaatg gcaagaagaa caaacgatat
    40501 agcgatagta agcaaacgtt cggtaagaaa aaagggaaga agccatttta caaaggtgtt
    40561 aaaaagaaga aaaagaaaaa ataaaatgag ttggaggaaa cagctatgcc aaaaggcgag
    40621 ggaaaattaa ttgcacaaaa caagaaagct cgccatgatt attcgatcat cgacacgatg
    40681 gaagcaggga tggtcttgca aggaaccgag atcaagtcga tacgaaacag ccggatcaat
    40741 ctaaaagatg gatttattcg cgtccgcaac ggagaagctt tcttgcataa tgttcatatc
    40801 agtccttatg aacaaggaaa tatttttaat catgatccgt tgcgcacgag aaagttatta
    40861 ttacacaaaa aacaaatcat ccggcttgaa aatgaattga aaaatactgg aatcactgtt
    40921 gttcctttaa aagtctatat tcgtaacggc tatgccaagg tattgattgg tctggcgaaa
    40981 gggaaaaaat cttatgataa acgggaagat ttgaaacgaa aagatatcga tcgacaaatt
    41041 gatcgaacat taaaaaattt ctctagataa tttcattatc ctttttcgca tattcggtgc
    41101 ttttgctttt acttaatgat aaaatttgtt agcatttaat ctgataaaac atgattatca
    41161 taggtttttt cttagatcag gagcaccgtg tataaagaaa aattcgcttc gtttttgact
    41221 gaaataaaaa agtgtctaat tttcatgaaa atgttttgaa ttttctgttt catggtggta
    41281 tgataccgtg tggttagaaa ccgattggaa aaaaccagat acgcacatga attgatttta
    41341 tgtgaatttc ggggaaaaga ggtgaaatgg attggatgaa cgctcgctaa cgttccacat
    41401 tggaccaatt tggtttgatg gtacagtttg tataatggtg ttactgacct gtttgattgt
    41461 tttcttctta gcttactttt ttacaagaaa tcttaagttg aaaccaacag gcaaacaaaa
    41521 tgcgatagaa tgggtcattg attttagtcg aggaatcgta acagataatc tgcctagaaa
    41581 agaattgagc aatttccatt tgttggcatt tacattgttt ttatttgttt ttgttgcaaa
    41641 caacatcggt ttagtaacaa aaatcgtttt acctaacgaa acaacacttt ggaaaagccc
    41701 gacagcagac ccgtttgtta ctttaacttt ggcatttata atgatcttac ttactcactt
    41761 atttggggtg aaaaagttag gcttcaaagg atatttcgta aattcgttct tgaaaccata
    41821 tagttttatg tttccgatga aactgattga agaatttaca aacttattaa cacttgcttt
    41881 acgtctttat gggaatattt atgcggggga agttttgcta accttgattg caaaaatggt
    41941 aataagctta ggctggttct cattgccgtt agcaattcca ttagagatgg tctggatcgc
    42001 attctccttg tttatcggaa gcatccaagc atttgtcttc gttactttat ctatggttta
    42061 tatgagccat aagattgaag tggaagaata atattcgttt taaaaacaaa ctactattta
    42121 tctaatttag gaggaaaaca attatgaatt atatcgcagc agcaatcgca atcatgggag
    42181 cagcaatcgg agccggttat ggtaacggtc aagttatctc taaaacaatc gaatcaatgg
    42241 ctcgccaacc tgaaatgtct ggtcaattcc gtacaacaat gtttatcggg gtagctctag
    42301 tcgaagctgt tcctatctta ggggtagttg tcgccttgat tctagttttt ggtgtttaat
    42361 tgaatcagta ttaaggcaga aacaatttgg tttgttactg cttttttaca tgaaaactag
    42421 aagtacttca aagaaaggaa ggctcgccta tgctgaatca attggcaatt gcagaagttg
    42481 gcaatgcgat gttaggaaat gtcatcgttg tcagcggctc atttttgata ctactggctc
    42541 tcttgaagca ttttgcttgg ggaccaatca gcgatatttt gaaaaaacgt gaagacaaga
    42601 tcgccaatga tttggattct gcagaacaat ctcgcatcaa ctcagcgaaa atggaacaag
    42661 aacgcgaaca acaattgtta gcctctcgtt ctgatgcagc tgatatcatc aaaaatgcga
    42721 aagaaagtgg agaattaagc cgccaaaata ttttgaagga tgctcaagaa gaagcagctc
    42781 gtctaaaaag taaagcccaa gctgatatca ctgtagaacg tgattcagcg ctgaactctg
    42841 taaaagacga tgttgcagaa ctatctcttc aaatcgcgga aaaaatcttg aacaaagaat
    42901 tatctccaga aatgcacgaa tcattaatca atcaatatat cgaaggtcta ggttcttcaa
    42961 atgaaactag ataaatatac agtaggaaga cgttacggga aagctttatt tgaactggcg
    43021 atcgattcaa ataaagcaga agaaatttat caagatctcg taagcttacg tcaaatctat
    43081 gaacaagtac ctggattagg taatatgtta agtgatgttc gtctagaacc tcatgaaaaa
    43141 cgagaaatca tggacaagct tgttagtggc tatgatggaa tcgttaaaaa ctttttagaa
    43201 gttgtttatg cgtataaccg aatggatgat cttttgttca tgatcgaaga atatgaacgt
    43261 cgatatgatg aacatcaagg tttgctgttg ggaagtgtca caacagcagt tccactttca
    43321 gacgaacaac gaatacaatt agaaaagaat gttgcaaaaa caatgaacta tcaatcagtc
    43381 gaattaaaac aaatcgttga ttcttctatt attggtggcg cgattgttga agctaaccat
    43441 cgagtaatcg acggaagcat tcgcacgcaa ttagaaaata tgcgtaatca attaaataga
    43501 tagtacaaag aggtaggtga atcgaatggc catcaaagca gaagaaatta gtgccttgat
    43561 taaagaacaa attgaaaatt atcaaaacgt gctttcagtc gaagaaatcg gtaccgtaac
    43621 ttatgtaggt gacgggatcg ctcgtgcaca tggtttagaa aacgcgatga gtggtgagtt
    43681 gcttgaattt tcaaatggct catacggaat ggcgcaaaac ttagaatcaa atgatgtagg
    43741 tatcattatc cttggcgatt tcgagtccat tcgtgaagga gataaagtaa aacgaacagg
    43801 aaagatcatg gaagttccag ttggagaggc cttgattgga cgggtagtta atccgctagg
    43861 tcagccaatt gacggactag gtgaaatcgt tactgacaaa gctcgtccag tagaagcaat
    43921 ggcaccaggc gttatgcaac gtaaatctgt taacgaacca atgcaaacag gtctaaaagc
    43981 aatcgacgct cttgtaccaa tcggacgcgg ccaacgtgaa ttagttatcg gtgaccgtaa
    44041 aacagggaaa acttccattg cgatcgatac gatcatcaac caaaaaggtc aagatatgat
    44101 ctgtatctat gtagcaatcg gacaaaaaga ttctacagtt cgtacacaag ttgaaacatt
    44161 gaaaaaatat ggcgcaatgg attacacaat cgttgtgaat gccggtgcgt ctcaaccagc
    44221 gccgttgctt tatatcgcac catatgctgg tactgcaatg ggtgaagagt tcatgtacaa
    44281 cgggaaacat gtcttgatca tttttgatga tctttcaaaa caagctgttg cttatcgtga
    44341 actttccttg ctgcttcgcc gtccaccagg tcgtgaagct tatccagggg atgttttcta
    44401 cttacactca cgcttattgg aacgtgcagc aaaattaagt gatgatttag gtggcggttc
    44461 aatgactgcc ttaccatttg ttgaaacaca agctggagat atctctgctt atatcccaac
    44521 aaacgttatt tcgatcactg atggacaaat cttcttagaa agcgacttgt tctacgcagg
    44581 tactcgtcca gcggttgatg ccggactatc tgtatctcgt gtagggggtt ctgcgcaaat
    44641 caaagcaatg aaaaaagttg ctgggacttt acgtctagac ttagcaagtt accgtgaatt
    44701 agaagcattt acacaattcg gttctgactt ggatgcagca acacaagcaa aactaaatcg
    44761 tggacgccgt acagtagaaa tcttgaaaca aaaattgcat gcaccgctag ctgttgaaaa
    44821 acaagtagtg attttgtatg ctttgacaca tggattctta gatagtatcc ctgtagacag
    44881 tattttagac ttcgaacatg aattattcga atatctggat accaatcatt cagatatctt
    44941 tgaaacaatc cgtacaacaa aagatcttcc agaagaagaa gcgttgaaca gtgcgattca
    45001 ggaatataaa gacatgttct tagctacaaa aggaaataat tcctctacag aagataaatt
    45061 gaaatccatt caaaatgcat agtgaggtga gatgaatggg tgcttcatta aatgaaatca
    45121 agacacgtat cgcttcaacg aaaaaaacga gccaaatcac gcgggcaatg cagatggtgt
    45181 ctgcttctaa gttgacaaaa tctgaagctt cttcacagaa gtttcaggtt tatgcaacaa
    45241 aagtaagaga aatcgttacg catttgactg ctactcagtt aaatgatatt gcctcagaaa
    45301 atcctagagg ggatatcaat tacaacagca tgttgctcag ccgtcctgtt aagaaaacgg
    45361 gttatatcgt catcactgca gatggcggtt tagtcggtgg atataacagc tcgatattga
    45421 aacaaacgat gtctatttta gaagaagacc accaatcacc cgatgattac gtgatgatcg
    45481 caattggcgg aactggtgca gacttcttta aagcacgcgg tatcaatcta gcatatgagc
    45541 tgcgcaatct atctgatcag ccaagctttg atgaagtccg caaaatcgta aatatggcaa
    45601 caactatgta ccaaaacgaa gtattcgatg aattatacgt atgttataac catcatatca
    45661 attcgctaac tagtcagttt cgtgtagaaa aaatgctgcc aatctcagac ttagatccag
    45721 aagaagcaac tactttcgaa caagaatata tttttgaacc ttcaaaagaa gaaatcttgt
    45781 ctcagttatt gcctcagtat gctgaaagtt tgatctacgg tgcgatcgtt gacgccaaaa
    45841 cagcagaaca tgctgctggt atgacagcaa tgaaaacagc aactgataat gctgctaata
    45901 tcattgatga gctgacggtt tcttacaacc gtgcaagaca aggggctatt acccaagaaa
    45961 ttaccgaaat tgttgccgga gcttcggcac tagaatagta ttgagattgg aggaaaaaag
    46021 atgagttcag gcaagattgt tcaagtaatc ggtcccgttg tcgacgtgga attttcttta
    46081 gaccaatcct taccagatat caacaacgca ttagttgttt ataaaaatga cgaaaataaa
    46141 tcaaaagttg ttcttgaagc agcgttagaa ttaggggacg gagtgatccg gaccattgcg
    46201 atggaatcaa cagatggttt acaaagagga atggaagtca ttgatacagg caaagcaatc
    46261 tctgttcctg taggtaaaga aacattaggt cgcgtattca acgtactagg agatacgatc
    46321 gatttagaaa cacctttccc agaagatgcg gaaagaagcg aaattcataa aaaagcacca
    46381 gcctttgacg aattaagtac aagtacagaa attttggaaa cagggatcaa agttatcgat
    46441 ttgcttgccc catatttaaa aggtgggaaa gtcggactat tcggtggtgc cggtgttggt
    46501 aaaaccgtac tgatccaaga actgatccat aatatcgccc aagaacatgg tggtatttct
    46561 gtatttaccg gtgtaggtga acgtactcgt gaaggtaatg acttgtatta tgaaatgaaa
    46621 gattcaggag ttatcgaaaa aacagccatg gtgttcggac aaatgaacga accaccaggt
    46681 gcacgtatgc gtgttgcttt gactggtttg acgattgcgg aatatttccg tgatgtagaa
    46741 ggtcaagatg tactgttgtt tatcgacaac attttccgtt tcactcaagc tggatctgaa
    46801 gtatcagcct tgttaggacg gatgccttct gcggttggtt atcaaccaac attggcaaca
    46861 gaaatgggtc aattgcaaga acgtatcaca tctacgaaaa aaggttcgat cacatcgatc
    46921 caagcaatct atgtgccagc cgatgactac actgacccgg cgccggcaac agcattcgcc
    46981 catttggatg cgacaaccaa cttggaacgt aaattaacag aacaagggat ttatccagcg
    47041 gtagatccat tggcttcttc ttcaagtgct ttagcacctg aaatcgtggg agaagaacat
    47101 tataaagtag caacagaagt ccaacacgta ttgcaacgat atcgtgaatt acaagatatc
    47161 attgctatct taggtatgga tgaattatcc gatcaagaaa aagtgttagt ttctcgtgcc
    47221 cgcagagtcc aattcttctt gtcacaaaac tttaacgtgg ctgaacaatt tactggtctt
    47281 ccaggttcat atgtccctgt agaagaaaca gtgaaaggat tccgcgaaat cttggaaggc
    47341 aaatacgatg atcttcctga agaagctttc cgaagtgttg gccgaatcga agacgtcgta
    47401 gaaaaagcga aaacactagg ctactagaaa ggggtgcccc atggatcaat atttaacagt
    47461 aaatgtggta actcctgccg gtttggtcta tgatcaccat gcagctatcg tcgttgcacg
    47521 tacgacagct ggtgagcttg ggattttgcc taaacatgca ccaatcatcg ttccattgac
    47581 gattgatgaa gttcgtgtaa aacgaacaga ttcagatacc cacgtcgatt ggatcgctgt
    47641 taatggcgga atcatggaag ttcgtgataa tgttgtttca atcgttgctg atagtgcgga
    47701 acgtgaacgt gatatcgatg tatctcgtgc ggaacgtgca aaacaacgcg cagaacgcca
    47761 aatcgaagaa gcaaaagcaa aacaagatac gaacgaattg aaacgggcaa ctgttgcgtt
    47821 gcaccgagca atcaatcgta tcaaagtatc taagcactca taaaatgaga tgcggatgtt
    47881 tatccgtgtt taagaacagt aaaaagaggt tatgacagaa gtggtcagct tcaagacata
    47941 agaaggaatt tacgaaaatt gcttctcaaa tttttgtgaa atctcagctt atttttcgaa
    48001 gaagctactt ctggaacacc gtatatcgga ggttgtgaaa tcagcgtttt gtcctgagta
    48061 ttagagaaac aaatggaaaa tagttcctca tattttgccg ttttttgagc taataccgag
    48121 ggtcagatga ttgaacaccg tttatttgga ataagggact gcgacaagac tttt
//
LOCUS       DACDIE010000015        47460 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.015, whole genome shotgun sequence.
ACCESSION   DACDIE010000015 DACDIE010000000
VERSION     DACDIE010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.015"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(45..779)
                     /locus_tag="IV250_000945"
     CDS             complement(45..779)
                     /locus_tag="IV250_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP8768245.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1106..1516
                     /locus_tag="IV250_000946"
     CDS             1106..1516
                     /locus_tag="IV250_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAP8768246.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1509..2210
                     /locus_tag="IV250_000947"
     CDS             1509..2210
                     /locus_tag="IV250_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAP8768247.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2210..3796
                     /locus_tag="IV250_000948"
     CDS             2210..3796
                     /locus_tag="IV250_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP8768248.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3815..4036
                     /locus_tag="IV250_000949"
     CDS             3815..4036
                     /locus_tag="IV250_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HAP8768249.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4033..4794
                     /locus_tag="IV250_000950"
     CDS             4033..4794
                     /locus_tag="IV250_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAP8768250.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5174..5683
                     /locus_tag="IV250_000951"
     CDS             5174..5683
                     /locus_tag="IV250_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP8768251.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5758..6297)
                     /locus_tag="IV250_000952"
     CDS             complement(5758..6297)
                     /locus_tag="IV250_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAP8768252.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6437..7048)
                     /gene="rpsD"
                     /locus_tag="IV250_000953"
     CDS             complement(6437..7048)
                     /gene="rpsD"
                     /locus_tag="IV250_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAP8768253.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7364..8161
                     /locus_tag="IV250_000954"
     CDS             7364..8161
                     /locus_tag="IV250_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HAP8768254.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPSATILV
                     EGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLGYEHVIANFPAFTLTYFAS
                     HGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            8189..8824
                     /locus_tag="IV250_000955"
     CDS             8189..8824
                     /locus_tag="IV250_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAP8768255.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8844..9617
                     /locus_tag="IV250_000956"
     CDS             8844..9617
                     /locus_tag="IV250_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8768256.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10025..10822
                     /locus_tag="IV250_000957"
     CDS             10025..10822
                     /locus_tag="IV250_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HAP8768257.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10800..11213
                     /locus_tag="IV250_000958"
     CDS             10800..11213
                     /locus_tag="IV250_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8768258.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11197..13842
                     /gene="mutS"
                     /locus_tag="IV250_000959"
     CDS             11197..13842
                     /gene="mutS"
                     /locus_tag="IV250_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP8768259.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13860..15968
                     /gene="mutL"
                     /locus_tag="IV250_000960"
     CDS             13860..15968
                     /gene="mutL"
                     /locus_tag="IV250_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAP8768260.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15990..16550
                     /locus_tag="IV250_000961"
     CDS             15990..16550
                     /locus_tag="IV250_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAP8768261.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16635..18623)
                     /gene="treP"
                     /locus_tag="IV250_000962"
     CDS             complement(16635..18623)
                     /gene="treP"
                     /locus_tag="IV250_000962"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HAP8768262.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18846..19559
                     /gene="treR"
                     /locus_tag="IV250_000963"
     CDS             18846..19559
                     /gene="treR"
                     /locus_tag="IV250_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HAP8768263.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19636..20082
                     /gene="msrB"
                     /locus_tag="IV250_000964"
     CDS             19636..20082
                     /gene="msrB"
                     /locus_tag="IV250_000964"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAP8768264.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20252..20854
                     /gene="ruvA"
                     /locus_tag="IV250_000965"
     CDS             20252..20854
                     /gene="ruvA"
                     /locus_tag="IV250_000965"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAP8768265.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20867..21868
                     /gene="ruvB"
                     /locus_tag="IV250_000966"
     CDS             20867..21868
                     /gene="ruvB"
                     /locus_tag="IV250_000966"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAP8768266.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21897..22475
                     /locus_tag="IV250_000967"
     CDS             21897..22475
                     /locus_tag="IV250_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP8768267.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22527..23009
                     /locus_tag="IV250_000968"
     CDS             22527..23009
                     /locus_tag="IV250_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HAP8768268.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23126..23500
                     /locus_tag="IV250_000969"
     CDS             23126..23500
                     /locus_tag="IV250_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8768269.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            complement(24178..25530)
                     /gene="brnQ"
                     /locus_tag="IV250_000970"
     CDS             complement(24178..25530)
                     /gene="brnQ"
                     /locus_tag="IV250_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP8768270.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
     gene            25677..26267
                     /locus_tag="IV250_000971"
     CDS             25677..26267
                     /locus_tag="IV250_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP8768271.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            complement(26395..27744)
                     /locus_tag="IV250_000972"
     CDS             complement(26395..27744)
                     /locus_tag="IV250_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8768272.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(27912..28652)
                     /locus_tag="IV250_000973"
     CDS             complement(27912..28652)
                     /locus_tag="IV250_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP8768273.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            complement(28665..30257)
                     /locus_tag="IV250_000974"
     CDS             complement(28665..30257)
                     /locus_tag="IV250_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8768274.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            complement(30825..31121)
                     /locus_tag="IV250_000975"
     CDS             complement(30825..31121)
                     /locus_tag="IV250_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8768275.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            31297..32022
                     /locus_tag="IV250_000976"
     CDS             31297..32022
                     /locus_tag="IV250_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAP8768276.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEMAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            32015..32857
                     /locus_tag="IV250_000977"
     CDS             32015..32857
                     /locus_tag="IV250_000977"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAP8768277.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELIALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            32860..33381
                     /locus_tag="IV250_000978"
     CDS             32860..33381
                     /locus_tag="IV250_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAP8768278.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(33634..34197)
                     /locus_tag="IV250_000979"
     CDS             complement(33634..34197)
                     /locus_tag="IV250_000979"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP8768279.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            34448..34717
                     /gene="rpsO"
                     /locus_tag="IV250_000980"
     CDS             34448..34717
                     /gene="rpsO"
                     /locus_tag="IV250_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAP8768280.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            34905..37031
                     /gene="pnp"
                     /locus_tag="IV250_000981"
     CDS             34905..37031
                     /gene="pnp"
                     /locus_tag="IV250_000981"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAP8768281.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            37541..37807
                     /locus_tag="IV250_000982"
     CDS             37541..37807
                     /locus_tag="IV250_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP8768282.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            37951..39942
                     /locus_tag="IV250_000983"
     CDS             37951..39942
                     /locus_tag="IV250_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAP8768283.1"
                     /translation="MATTHNTKKISMTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            40296..41597
                     /locus_tag="IV250_000984"
     CDS             40296..41597
                     /locus_tag="IV250_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP8768284.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(41727..43097)
                     /locus_tag="IV250_000985"
     CDS             complement(41727..43097)
                     /locus_tag="IV250_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAP8768285.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVNPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(43279..43602)
                     /locus_tag="IV250_000986"
     CDS             complement(43279..43602)
                     /locus_tag="IV250_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAP8768286.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            complement(43927..44535)
                     /locus_tag="IV250_000987"
     CDS             complement(43927..44535)
                     /locus_tag="IV250_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAP8768287.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            44865..45581
                     /locus_tag="IV250_000988"
     CDS             44865..45581
                     /locus_tag="IV250_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAP8768288.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            45594..46289
                     /locus_tag="IV250_000989"
     CDS             45594..46289
                     /locus_tag="IV250_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAP8768289.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            46373..47002
                     /gene="udk"
                     /locus_tag="IV250_000990"
     CDS             46373..47002
                     /gene="udk"
                     /locus_tag="IV250_000990"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAP8768290.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
BASE COUNT        15383 a         8787 c         9878 g        13412 t
ORIGIN      
        1 ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc cataaataat
       61 aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac tggtacccat
      121 gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg tacaagacgt
      181 ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag tgaagcaact
      241 aatgtccata caaggaaacc aagtgctaag tgagctgtac caagattatc ttggaagaat
      301 ggtgcttttg taatacctag ggcaccaaag aataaaacga atgaaccaaa gaattcattg
      361 acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc taacacatgg
      421 tttacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat caattgtcca
      481 atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca agggaataga
      541 ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct gattccacca
      601 aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag catccagtca
      661 ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt acctagaacg
      721 attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga gtaatccata
      781 aatacctcca aaagttataa aaatgcaaaa aaaaaagaaa catcaacacg aaaagcatcg
      841 gtgcagtgtt tcgtgtcgga aggatttctg cagtcttaac aaatgtgaat aaaaaaatga
      901 attccattac ttataagaaa ccagaccaac agtaaaactc caactctttt taaaagttct
      961 ccacaaatca ccaagcttaa ctataaatat accaaaaaaa aaacgttttt tttctcaaaa
     1021 attgtatctt agaaacagca tgttttgaaa ctttcagaaa aggtttgtta gtaaaatgaa
     1081 agctatacgt aaggagtgtt gtttgatgaa attttttcaa gagtttttca ttattatttt
     1141 cttttctgta ttaggagaag gcatacggat ttttgctcat ttacctattc ctggaagtat
     1201 cttaggtatt ctttttttat ttttagcatt tgaaatgcat cttgttgatc ctgaaaaaat
     1261 cggagcaact ggtgattttt tattgaataa tctagccatt ttatttgttc ctgctggtgt
     1321 aggtttattg gaatattttg atgatattgc ttcgatatgg cctgttttac tgggagcagt
     1381 cgtcgtgtgt tccatcgtca cgatggttgc agcgggaaaa acggcagaag gtgtagaagc
     1441 tttgctgggc tatgtccgta agaaaaaagc agaaagggta tctgtaaacg aaatggagga
     1501 gaatcaaatt gactgagttt tttgtgaatc cattatttgg cttaagtttg actttaggag
     1561 tgtacgtatt atctgacaaa tttttgcgga aatataaact gcctgttttg aatcctctcg
     1621 tattatcgat tgttgttatt atcttatttt tgcatttttc tgggatcagc tataaagact
     1681 ataatatcgg gggcaatttt ttgactatgc tgattactcc tgcgacagtg gcacttgcca
     1741 tccctcttta taaaaacttc cgtttactga aagagaattt ttatcctgta atggctgcga
     1801 ttttagcagg aatagtcgca aattgcttgg ttagtgtagg gttcggttac ttcttctctc
     1861 ttcataaaga gatggtgatc tcattactac ctaaatctgt aacgacggct atttctgtcg
     1921 atttatctca tacgatgggc ggggtgaatg ccgtgacact tgctattgtt gtctctacag
     1981 gtatttttgg ttcgctgatc gccactcata ttttccgcct attcaagatc gaaagtccag
     2041 ttgctcgcgg tgtggcgtta ggaagtactt ctcatgctat tggtacagca aaagcaatcg
     2101 agattggtga aatagaagga atcatttccg gacttgcgat atgcgtcaac ggaatattga
     2161 ccgttttatt gctaccgtta ttctttcagc cttttgctgg cttgttttga tgcgaaaacc
     2221 actattttat gctacaataa ccaaaacgtt cgagcataaa aaaggaggga aagacgtgta
     2281 tttgtcttcc gagtatctta aacaatttga taaagatcta tattataaga ttctcctcct
     2341 agaaagtttt gaggatcaag cttggcatac cgctgcacaa ttagcgcaag ttgttcagct
     2401 ggatgcccgc agcgtgtcga aatacctgaa cgagctgtct aaaaactatc agcagtttag
     2461 cggtaagaca caccctttgt ttacaaaaaa tcaccgttct ggttataact tttatgatac
     2521 gctcgattca attgaacatg agcgtttttt gatctatctc gtgcaaagta cgctgaaatt
     2581 ccagttgctg cacgacattt tttttgaaga attccataca atgtaccaat ttgcgcaaaa
     2641 gcattacatt agtgaatcta ctgctcatcg aaaaatcaat gaatggaaac agcaactgca
     2701 aacttacgga attcggctgc aacgaggtac atatatcgct caaggcgaag aagaaatcat
     2761 tcgactttac ttgcatatga cattctggca gttgtttcga gggaaaatct ggccatttga
     2821 aacgatttct caaatggacg taaagaatat ggcagaacat atcatggctt tttttaatgt
     2881 tcgtctgaac gagatcaaaa aaagacgatt ggaatatatg ctaggtgcct tttttttaag
     2941 aaaatcacag aagcattatg ttgtattgaa tgagaaaaaa agacggctga tttctgacaa
     3001 tctgttgttc cagcgatttt gccaggtcat ggagccggta tttcctaatt attttcaagt
     3061 agaagacgag ttaggcgcat tatttcttgt actgatgact cgtgaagaat attatagtga
     3121 tcctaagata cggaagaaaa tttttgattt tcatcaacaa gcaaaaacac cgccctttac
     3181 agccctttca gaagcaaaag ccgctctttc tctttatcag gaagagcagg ggctgccagc
     3241 agaaaatcta accttcgagg cagaaaacta tttattcagc agtcattttt tcgcctatct
     3301 ttttcctaat gcgaaagaaa cgattgatgg caatagtagt gattttatta atcatttagt
     3361 tattgaaaac aaggaattga aacaatggct cgtacatttt tttgaaagta gacacaaaca
     3421 tcccaatcat ctggcattta aaaatcatgc gtttttgatg gggcggtatc tgactgtgtt
     3481 taaaacatta ggagccttta ctccacagct tccaaagatc acgatccttt taatgacaga
     3541 tttcccatta tttgaagagc aactgttaga ggagggatta cggacatttt tccgtaatga
     3601 gtatcagctg atttttctgc caacagatta tcgggggaga gaagttgatc ttctgatttc
     3661 gacaagtaaa gtccaccgca aaccatgggc ggatcttgac tattttattg tgactgaaga
     3721 actaaaatta attgattaca tccaactatc tcaaaaattt gaaatgattc aaaaacaaaa
     3781 actattaaaa caataacaaa gaaggagaaa aaagatgaca tttgaagaaa ttttaccaga
     3841 attgaaaaaa ggtgcaaaga ttattcgtaa aggatggagt ggatttgaac tttatgttgt
     3901 tctcgtatcc gatgatgaat ttgatggctg tccggtgacg ccgtacttat tgatcaaaac
     3961 aagtgacgaa ggtttttcca gcttcgctcc tacagtttgt gatattttag cagatgattg
     4021 ggcgatcgta gaatgaaaaa ccagtatcca gatctagcta aacaggttgt atttatcaca
     4081 ggagctgctt ctggaattgg tgctgcgcaa gcccgagcgt ttcttgaaca agatgctttt
     4141 gtatttggat tagatcaaaa gttcggtaaa atggaagaaa tttctttgcg atttcctgaa
     4201 acttttgcct acgcactagg tgatgttcga caaatgaaag aactccaaca agcggtatcg
     4261 aaatgccaaa atcattttgg tgaagttacg attttactga atacagcggg gatattggat
     4321 gcctacaaac ccttgatgga aactgacgaa tcgctgtggg atctgattta tgagacgaat
     4381 gtgaaaagca tgtatcagct gacaaaaatc atccttcctg atatgatccg ccaaaaaagc
     4441 ggaacgatca tcaatatggc ttcgattgct ggcttgattg ctggtggagg tgggatcgct
     4501 tatacaagcg cgaaacatgc gattgtcgga tttaccaaac aactcgctct ggattacgca
     4561 ggacagggta tccgagtaaa aggaattgct ccaggagcca tccaaacacc aatgaatgcg
     4621 gctgattttg aagggaaagg tgagatggca gagtgggtgg caaatgaaac accagtcaag
     4681 cgatgggcac agccggaaga agtagctgag ctgacgttat ttcttgcctc tcctcaagca
     4741 agctacatac aaggaacgat catcccaatc gacggcggat ggttgttaaa ataaccttgt
     4801 gtttttttca agcttctgta tgataaaact aaagagagaa acaacggttg atgataaaaa
     4861 aagtcgatga ctgttcccga cattcgatgc cgaacagcca gttcaaaaaa ttctttttaa
     4921 taatcgttaa tattcgaaac taagcggctt ttcacgacct cttgatataa acgatattca
     4981 aaagcttgca actgcggtaa caagtccagc caaatgaaaa atataaggag ctatttttat
     5041 ttgaccattc ttattacttg ttgcagggca gctttttcac taccttctgc gacacttcag
     5101 ttggaatttt cttcccatcc tttggtcgcg gtcattacca agataaaatt atcttgcaaa
     5161 ggagaaatag aacatggaaa aaaataatac ccgttcagcc aaacgctgga caacgacaga
     5221 tgtgactcgg atggcgctag tcactggttt atatgtggca gtgacgctgg ttttgtctgt
     5281 ctttagcttt ggagtgattc agattcgtct ctcggagatg ttcaactatc tgtccttgta
     5341 taacaaaaga tacatcgtcg cggtaacagc gggagtagct ttggctaata ttgcttctcc
     5401 tcttggactg atagatgttg cagtaggaag cgtcagcacg ctgcttgtat tgcttatcaa
     5461 ctataaaatc actagtcgca tcaaaaacat gaaaatcaaa atggccgtga cagcatgcgt
     5521 atttgctttt tccatgttta cagtagctgg tcagctaacg attttatatc aactaccttt
     5581 tttcttgaat tggtgggtga ttgctttagg agaattggca tcaatgattg tcggtgggat
     5641 aattatttat tggatcggta aaaaagtgga tttgtcaaaa taaagatctg tttaatttaa
     5701 aatagggcag catggaagag actgaatgtt gagtcttctt ccatgctgct tttttgatta
     5761 atttgtatga agaaatcgat ttagccgttt tttcatcaag ataccgatat aagctgctcc
     5821 agttgccttg attgcttccg gcagtaaaaa cggcagtagt ccactggcaa atgcagttgt
     5881 aaaagtaaga tcaccactta ttttcagcca gatcgttccg cagatcaatg cagcaagaga
     5941 gcccattaga ttggcaaaga ttgcatataa gtaatgagca tccgttcttt cgatcatcca
     6001 accagttatc cacgtttgta agagaaatcc aatcaaatat ccgccagttg gcccaaataa
     6061 aacagctata cctccagtca tccctgaaaa tacaggaaga ccaatcaagc caagtaaaag
     6121 ataaactcca acagatatca atcctagttt tcttccgaga aaagttgctg ttaatccaat
     6181 agcaaatgtt tgccctgtca aaggaaccaa accaaaagga atggtcatct gagaaaggac
     6241 agaaataatc gcggcaaatt ccgcagcaag tatcaattct ttcaatgttc ggttcataac
     6301 gccctctttt cgttaactta atatttttat ttggttaacg aaattgtaaa acaaagtgat
     6361 tactttgtaa atagaaaata taaaaaaaga ccagctatcc aatagatagc ggtctttttc
     6421 aagaaagctt ttgaaattat aagctttggt tgtagtattc aacgacaaga gcttcgtcga
     6481 tttctggata tagttcgtca cgttctggta aacgagtcaa tgaaccttcc aatttttcag
     6541 cgtcaaagct tacgaatgct gggcgtccaa cagttgcttc tacagcttct tttactgtta
     6601 cgatgttttg tgatttttca cgaactgaga tcacttgacc cacttctacg tggtatgaag
     6661 ggatatcaac gcgtttgcca tcaactaaaa tgtgaccatg gttaactaat tgacgtgctt
     6721 gacgacgagt cgttgcaagt cctaaacggt aaacaacatt gtctaaacgt tgttctagta
     6781 agatcatgaa gttaacacca tgtttacctt ctttgatttt gctagctttt acgaacaagt
     6841 tacggaattg acgttcgttc attccgtaca tgtgacgaag tttttgtttt tcagttaatt
     6901 gcatgccgta ttctgattgt ttgccacggc tattaggtcc atgttgacct ggtgcgtaag
     6961 ggcgacgtgc taattctttt cctgtgcctg acaatgagat gcctaagcga cgagaaattt
     7021 tccatgatgg tcctgtataa cgagacatta aaatttcctc caatgaatat aagtttttgg
     7081 agtaaaataa tccgttgaaa aattcataat catatagttt gttcttcaat cttcaccttt
     7141 gcagccgcgg ttacacaatc gaacccgaga cggggcaaca aactagggat gagttattat
     7201 ttttctgctg cattatttta cacaaattac agtatatgat agatcatcca cgaatgtcaa
     7261 gataattagt acataaaaac gctttacaaa cctaggctgt ctttataaaa tgagagggta
     7321 tgtgcaaatg gaaaaaagcg aaggaagttt aaggagaaaa tcaatgaatc cggtatcacc
     7381 attatttgag aaaatcgatg cttcaattga aaagaaggcg aatctttttg aaaacagctt
     7441 ttcacgttac gctgttcgag cggttctcgc ctgtttgttt ttgactttag gaacggctgt
     7501 ggcatttgcg atcgcgatga agggggaaga tatcgcgcat ggtctaggaa agattttata
     7561 cgcatttatg ttcagctggg gtctggtcat gattctatac atgaacgccg aattaggaac
     7621 atcgaatatg ctttatatga ccgtgggtgt ttttcggcag agacttgctt taagtaaggc
     7681 agccaagatt ctatttgcct gtattttttt caatatgatt ggcggtatat tatgtggatt
     7741 tctaatttct atgactacgc catttcaaga tttaacgttg gacaacttta tgttagaatc
     7801 aatagcagta aaactagcaa aaccttcagc aacgattttg gtggaaggga tgtttgcgaa
     7861 tatcgttgtc aataccgctg tactgatcag catgcggatg aaagaggatg tcggaaaagt
     7921 gatcacagtc gtattcatca ttttcatttt tgctttttta ggatatgaac acgtgatagc
     7981 gaattttcct gcctttacgc ttacttactt tgcttctcac ggacagatgg atggaatgac
     8041 agtcagcaat gtgttacata atttgttttt tgctttggca ggaaattata ttggtggcgg
     8101 tttagtaatg ggactgggat atgcttggct gaatcagtca aaatcatcct atgtggacta
     8161 aaaagtcgtt cattaaaggg gaagtgccat ggaagtttta gtttttccaa aaaatgagga
     8221 aaaagcgtta caaatggaaa agtacatgaa aaatcttttt tcctttgctg gagtacctaa
     8281 gccggaacgc gctcttttac aacaaccgtt actggatgag agcaaaaatt tatctatttc
     8341 tgaagtttta gcttttgttc attattatta tgaacaaatc gaaagagagt atcagtatgt
     8401 agcgatcgat ttagtccaga aaaacatcaa acggcttaca tttgaagact tagtcgtatt
     8461 gttgccacta gttcagaaaa aagaatggtg ggacagcatc gactcttgga gaaaagtata
     8521 cagcacatgg tgtaaagcct acccagagca gcgtgaagaa gtctttcaat tgtttgaaaa
     8581 aaaggaagat ttttggctac gtcgaatagc aatcacgttg caattaggtt ataaacagca
     8641 aacagacaca ggactattga tccgagcaat agacgaagat cgtcaaacaa aggaattctt
     8701 tatccaaaaa gcaattggtt gggcattacg cgattatagt aagaccgatg ccgattgggt
     8761 aaaaagacag ctgacaacag atttgagtaa gttagctgtt cgagaggcaa gtaagtattt
     8821 ataagaaagc gagacgacaa gagatgagac gcaaattatt tgcttttgat attgatggaa
     8881 cgttgttaga ttcaaacaaa caagctttag atagcacaag agaagcattg gaaaaattac
     8941 gcaaagccgg tcatctggtc acgattgcta cagggcggag ccgatttcat gcgcaagaga
     9001 tcattcgcga tcttgatttt acgaactaca ttttatgtaa tggagcggcc ggttttttag
     9061 accatgaaca agtatataag aatttactag atcgcgacca gttggatgcg ttcgttcaag
     9121 aagcgcatca tgagcagatc gatacagctt ttgtcagctt agacaatatc aagcgttctt
     9181 cttccaatga tgtaacggtg atggaagaag cgatgcattc tttcggtgct gtcttgcctg
     9241 aattagatca gttttttgca gaggaacaag aaatatacca agcgttagca tttttcgatc
     9301 aaacatatga tgggaagttc tcttcatatg ataaaattcg atttgtccgc tggcatgaga
     9361 acagtgtaga tgtcgtacct aaaaatggtt ctaaagcagc gacaattctg cacttagcag
     9421 aacaagtagg catccctcat gaagacatca tcagctttgg cgatggacaa aacgaccgtg
     9481 aaatgttgcg catgtcagga attgggatag ctatggggaa tgctgtgcca gaagtacaag
     9541 cagaagctaa gatggtcact gatacaaacg atcaggatgg aatttggaag gcattgaaag
     9601 agttaagcgt gatttaaaag aggttgtgaa aaagctgttc tgcatcaagt aataagccca
     9661 gccaaacaaa aatagctcct cctatttttc gtttttctgg gcttattacc gcagatgcaa
     9721 gctttcgaac accgtttaag gaagaggtcg tgacagaagt ggtcagtttc aagaaataag
     9781 aagggattta cgaaaattgc ttctcaaatt tttgtaaaat tacggcttat ttctgaagaa
     9841 gtagatgaaa caacgtttat tcgggaaatg ggactgtgac aagatgtttg tcatagtccc
     9901 atttttttat tttatttttc aaaactaaga aataccagga aagagttcct tttgtttgac
     9961 cttttctttc ttattgaaaa caactataat atagagtgat gttaaaagag aagaggaatt
    10021 tagtgtgcgg gtattgttta ttggtgatgt agtaggttca atgggacgag agatgattac
    10081 tgaatatctt cctagattga agaagaagta ccgtccgcaa gtaacgattg taaatggtga
    10141 gaatgccgct tctggacgtg gaatcacaga aaaaatctat aaaaaatttt tgcaagacgg
    10201 cgtagatgtc gtgacgatgg ggaatcatac gtgggacaac cgcggaatct ttgagtttgt
    10261 aaatgaagct aaaaagatgg ttcgtccggc aaattttccg gaagggacgc ctggacaagg
    10321 gatggtcttt gtcaaagtga accaagtcga actggcagtg atcaacatgc aggctcgttc
    10381 ttttatggtg gacttggatg atccttttcg taaaatgaat gagctggtag aagaagccag
    10441 aaaacgtacg ccgatcattt ttgtagattt tcatggagaa acaacaagtg aaaaacaagc
    10501 aatgggctgg tttttagatg ggaaagtttc agcagtggtc ggtacacata cacacgttca
    10561 gacaaatgat gcgcgaattt tgccaagagg aacagcgtat ctgacagacg ttgggatgac
    10621 aggtccctat gatggtattt tgggtatgcg cagagaacct gtaatcgaaa agtttctgac
    10681 tgcattacca aaacgttttg aagttgtgga acaaggccga tcgattttaa gcggctgtat
    10741 cttggaattg gatgatacga caggtcatgc aaaagaaatc cagctgattc agatcaacga
    10801 tgatcgaccg ttcatggagt agaaagagag gcagtttttt gcagcaggag ccggaaattc
    10861 aaaaagaagt aaggaaactg acaaaacttt taagagagaa tgaaacgatc attcgctaca
    10921 aagaacttga agaaaagatc cagcaaaacc agtatttagc tgagttgaga gaaaaaatca
    10981 aacaagcgca aaaagatgct gttcattttg cccactatga caaaccagca gcagaaaaag
    11041 aagcaatcaa acaagcggat caattcatgc aagaatttga tcagcatccg cttgttgtag
    11101 cttatagaaa acagctgttg gaagcagatg atctgcttca tcatttaaca actatgatac
    11161 aagaagaaat caatggacaa atagaggagg aaaaacatgc ctcaaaaaac taaaaatact
    11221 cctatgatgg aacaatatct aagcatcaag gcccaatacc aagatgcttt tttgttctat
    11281 cgtttaggag acttttatga actattttac gaagatgcga tcaaagcgtc acagcttttg
    11341 gaattaacgt tgacgagcag aaatcgaaat gcagaagaac ctattcctat gtgcggtgta
    11401 cctcaccatg cagcacaagg atatatcgat gccttgattg aaaaaggcta taaagtagcg
    11461 atttgcgagc aggtagaaga tccaaagacg actaaaggga tggtcaagcg cgaagtgatc
    11521 cagctagtca ctcctggtac agtcatggac agcaaaggat tgtcggcaaa agacaacaat
    11581 tttttaacag ctgtagttag tcaaggaaat gaatttggct ttgcttatgc cgatttaagt
    11641 acgggagaat tgaagacggc tcggttgcat gatgaagaag ctgttttaaa tgaagcctct
    11701 gcattgcaga caaaagaact cgtattaggc agtgagatca ctgataccct tcgtgaacag
    11761 ctaagcgggc gtttagggct agtcttttct caacaaaatg agatggaaga aaacgcggaa
    11821 tttagctttt tgacaagcga actagtcgat ccgctggaaa aagaagcaac cggcaaacta
    11881 ttgacttatt tatcggtgac acaaaagcgg agtttagctc atatccaaaa agcagtagaa
    11941 taccaaccag atcatttttt gaaaatggat tattattcaa aattcaacct agaattaagc
    12001 caatctattc ggactgggaa aaagcacggc actcttctat ggttgttaga tgaaacaaaa
    12061 acagcaatgg gtggccgttt actaaaacaa tggctagacc gtccgcttat ccaggcaaaa
    12121 caaattaaag caagacaaga aatggtcgct tctttgctag acagctattt tgaacgtatc
    12181 gatttgcagt cagcattaac gaaagtctat gatctggaac gcctagcagg tcgagtagct
    12241 ttcggtaatg tcaatgggcg agacttgatc cagctgaaaa cttctttaga acaagtgcca
    12301 atgatcagag ggttgattga aggaatcgat caaggtcaat ggcaaagtct gctttcagaa
    12361 atgcagccga tggaccatct cgtccaattg atcgaccaag ctatccaaga tgatccgccg
    12421 ttacaaataa cggaaggaaa catcatcaaa gatggattca atgagcagct cgatatgtac
    12481 cgttctgcta tgagaaacgg aaagaaatgg ttggcagaac ttgaagcaaa agagcgtcaa
    12541 gaaacaggaa tcaagaactt aaaaatcgga tttaaccgcg ttttcggtta ttatatagaa
    12601 attacaaaag caaatcttgg aaatgcagaa ctggagaagt atgaacgaaa acaaacttta
    12661 gcaaatgcag agcgtttcat cacaccagaa ctaaaagaat tggagacaca aatcctcgaa
    12721 gcagaagaaa aatctgttga tttagaatat cagttgtttt tagctgtgag agaagaagtc
    12781 aaaaaggcta tccagccgct acaagtactt gcaaaagcaa tcagtgcagt agatgtgcta
    12841 caaagttttg caacgatcag cgaacgctat cagtatgttc ggccagaact tgtcagtgac
    12901 aagcatcagc ttctgatcaa agacggacga catcctgtag tagaaaaagt attaggacat
    12961 caggaatata tccctaacag cgtagaaatg gcagaagacg aaatgatctt gctgattaca
    13021 ggaccgaata tgtctggtaa aagtacgtat atgcgacagt tggcattaac tgttctgatg
    13081 gcgcaaatgg gctgtttcgt tccggcaaaa caagcagttt tacctatttt tgatcggatt
    13141 tttacccgaa ttggtgcaag tgacgactta attgctggac aaagtacttt tatggtagag
    13201 atgatggaag ccaatcaagc cctgcgttat gctacgccaa acagtttgat tttgtttgac
    13261 gaactagggc gaggaacagc aacctatgac ggaatggcgt tggcccaagc aatcatcgaa
    13321 tatatccatc gtcatgtaca agcaaaaaca ctcttttcta ctcattatca tgaactgacg
    13381 gtacttgaga aagaacttcc gcagcttaaa aatgtccatg ttggagccgt tgaaaaagac
    13441 ggagaagttg tcttcttgca taaaatgatg gatggacctg cggataaaag ctacggcata
    13501 catgtggcaa agatcgcagg attgccttcc aatctactag aacgagcagc agatatcctg
    13561 catacgctag aaaatggcga taacggacac catactgttc cagctgaagt agaagagaaa
    13621 actaaaacag ccaaacagga gcaaataaaa ccacctgcag aaaaagtaaa tcaagaccat
    13681 atacaagacc gtgtaaaaga agaaaaccaa cagctttctt tattcagtga attatccgaa
    13741 aatgaaacag aagtcattga atcactgaag caaataaatt tactagaaat gacgccaatg
    13801 gatgcactaa acacattata tgaattacaa aaacaactgt aggaagaagg cggatatcga
    13861 tggccaaaat ccaagaatta tctgaacgtt tagctaatca gatcgctgcg ggagaagtag
    13921 tggagcgacc agcttccgtt gtgaaagaat tagtggaaaa tgcaattgac gccggcagta
    13981 ctcagattga tatcttagtt gaagaagcgg gactgcggac gatccaagtg attgataacg
    14041 gagaaggcat tttggcagac gacgtagaga atgccttcaa gcggcatgcg acaagtaaga
    14101 tccataatcg cgacgatctt tttcgcatac gaacgctggg ctttcgaggg gaagctttgc
    14161 caagtattgc ctctgtttct gaaatgactc tggaaacggc gaatcaggaa gaaaaacaag
    14221 gcacctatct gtcgctgaaa ggcggagaag ttattgagca tcgaccagca ccactgcgtc
    14281 aaggaacgaa gattaccgtc agcaatctgt ttttcaatac gccagcccgc ttgaaatacg
    14341 tgaaaacact gcacacagaa ttagcgaaca tcggggacat cgtcaatcga ctggcattga
    14401 gtcatccatc gattgctttt cgtttagtac atgatggaaa caaaatgctg gcaactgctg
    14461 gaaatggcga tctgaaacaa acaattgcgg gaatctacgg attatctacc gcaaaaaaaa
    14521 tgctgaaaat cgaaggaaaa gatctagatt ttgagttatt tggctatgtt tcattgcctg
    14581 aggtgactcg tgccagtagg aattacttat cgacgatcat caatggacgt tttatcaaga
    14641 acttttcctt gaataaagca atcgttgcag gttatgggtc aaaattaatg gtcggtcgtt
    14701 tccctattgc tgtattggaa atcaaaatgg acccgctttt agtagatgta aatgttcatc
    14761 cgacgaagca ggaagtccgg ctgtcgaagg aaaaagaact aacagagcta attagtaacg
    14821 ccattcaaaa agcattgtct caagaaaatc tgattccaag tgcagcggat aatctacggt
    14881 ttaagaaaaa agtacctgat cagcctaaaa cagaacaaat cgaaatcgat ttgtcctacg
    14941 cttttgaaga agaaaaacct aaaaagccaa gtagtttgaa ttttgatcga gagacaggta
    15001 cctttttcgt agaagaacta aagaacacat ttgttgacag aattgttgat aagtctgtgg
    15061 aaaatgttga aaactattct ggtttttctg aagaaaacac cacttatcca gaaaaaacag
    15121 cgaaaacggt ggataagttt ggtgaaaatg ttgataactt tgtagaaaaa acagtggaaa
    15181 aatctgaaaa gaaagaattt cccattgtgg ataacatgga gaaaacaaat gaagaacaag
    15241 cagataaatc cgtagaagag gatgaaaaga tttacgagga agaattgaca ctccatcctg
    15301 aatttgattt cactcaagga aacgacaaaa cattagaaca agccatcaag aaattagaaa
    15361 cggaaaaacc agtacaacgt tttcctcagt tggaatattt tggacagatg cacggaacgt
    15421 atctttttgc ccaaagtagc gatgggttgt acatcattga tcagcatgct gcccaagagc
    15481 gcattaagta tgaatatttc cgggaaaaaa ttggggaagt atctgatgat ctgcaagaat
    15541 tgcttgttcc atttgtgtta gactatccaa acagtgatgc tttgaaatta aaagagcaaa
    15601 aacacttgct agaagatgca gggatttatt tagaagaatt tggtcaaaac agctttattg
    15661 tacgggccca tcctacttgg tatcccaatg gagaagaaga atcgattatt cgggagatga
    15721 tcgatatgct gctgacaact ggaagtgtga gtgtaaaaaa attccgtgaa gcaacagcaa
    15781 tcatgatgag ttgcaaacga tcgattaaag ccaaccatta tttgaatgaa gcacaagcac
    15841 gtgtgctgct gaaggatctg gcacgatgcg aaaatccttt caattgtccc catggccgtc
    15901 cagtattgat tcactttacc aattcggata tggaaaggat gttcaaacga atccaagacc
    15961 cgcattaagt caaagagaag agggaaaaca tgaagattgt attagcatcc caatcaccaa
    16021 gaagaaaaga gttattggct catatcgttt cagaattcga gattgaacct gctgatatcg
    16081 atgaaacgcc atttccagac gaacagcccg tcgattatgt tcgtagaatg gctgaagaaa
    16141 aagcacgaac tgtctgggaa caagatgaaa aagaagacca tttagtgatt gccagtgaca
    16201 cgactgttgt tctcaatcaa gagatcatgg gtaaaccaga agatctggct gaggctgaat
    16261 tcatgctgaa aaaactaagc ggggaaacac atactgtata tacagctgtt gttttaaaaa
    16321 caaaggaacg agaagaacgg atactagcag aagcacatgt cactttttat cctttgacag
    16381 acgaagagat caaacgctac ttggagacgg gggattatgc ggataaggca ggggcatacg
    16441 gcatccaaaa tgctgcgagt gtattcgtca aagaaatttc tggagattat tacagtatcg
    16501 taggatttcc gattggtgct gtcaatcagg cactgaaaac attcatttaa acaaaaaagc
    16561 ggactatctg attttcgatt gaaaatccat gatagcccgt tttttttatt gaaaagatta
    16621 cttatctact ctgactaaga ttttacttca gctttaccga ctgcttcgcc cgggcgaaca
    16681 agtcctgatt ggtcaagaga aagtcctgca actttttcat tattggtcaa agcaacaata
    16741 atgtcagttt tcttgccagc ttgttctatt tctggcaaat ccatttcagc aatcagggtc
    16801 tctggtgtga caaggtatcc ttctttcacg aatacattga aaggaacacc ctttagttca
    16861 acagtatcta atcccatgtg aacaagtact tccagcccct cattagaaag aataccaatg
    16921 gcatgttttg tttcaaagac actcgtcact ttaccgttta ctggtgcata gactttgccg
    16981 tttgacggtt cgacagcata accgtcaccc atcatttttt gtgagaaaac tgggtcattt
    17041 acttcagtta tttccttgat ggtccctgca gcgactgcga ataatgtttc ttttgttgca
    17101 actgccgtac cagttgctga aacaggttcg aaagactgtg gcgttgctgt tgcgccattt
    17161 gctgtttgaa gttgtacatc agcagcattt tcaggaacag caggatcaat cttattcaaa
    17221 attccctgac gtctaaagat gatcgtcaag ccaaatggaa tgatgacagc aatgatcatt
    17281 gcgatgatga aaggtaccca tgtttctgct tggatagcta gtattcctgg caaaccgcct
    17341 actccgatcg cgttagctcg aacacccatc aagttagcaa acatgccagc taatccagag
    17401 ccgaccattg cagcgacgaa aggatagacg tatttcaaat tgattccgaa catagcaggt
    17461 tcagttacgc ctaagtaaca agaaatcatt gctggaatcg agatttgttc ttctttttta
    17521 ttcccacgat gtaagaagat gatggctaag actgcagagc cttgggcgat atttgataaa
    17581 gcaatcatcg gccataaatt cgttgatccg aaatctgcaa tcagctgcat atcgatggca
    17641 ttggtcatat ggtggagacc agtaatcact aaaggagcat acaggaatcc gaatacggca
    17701 ctaaacaacc agctgataga agacgtcaat ccagtattta cgatatttga gatccagctt
    17761 ccgactgtcc agccgatagg acctaaaata acgtgtgcag ctaaaacagt tggtaacaat
    17821 gaaaacaatg gaacaaaaat catcgagatc gactgaggga tatatttgcg gaagaagatt
    17881 tctagatacg ctaacataaa ccctgccagc atggctggaa taacttgcgc ttgataacca
    17941 atcatctgga cttgagcaaa accaaaatcc caaaatggaa tatctgctgc ggctgtggaa
    18001 gctacactat aagcatttaa taattgcgga gaaactaacg taattcctaa aacgattcct
    18061 aatatttgtg tagtacccat cttttttgca atactccaag tgatacctac tggaagaaaa
    18121 tggaaaattg cttcaccggg taaccacaaa aaggcattga caccattcca aaacttcgag
    18181 tgttcaacga ttgttccacc aagactttca aactggatac cttcaagtac atttctgaat
    18241 ccaaggatca atccaccgac aatgattgca ggaatcagtg gtgtaaagat ttctgctaaa
    18301 acggcaatag cacgttgaac gggatgaagg ttttgttttg cagctgcttt tccttgttct
    18361 ttggaaaccc cttcaactcc agatactgca acgaagtcgt tataaaaagt agaaacatcg
    18421 tttcctatga tgacttggaa ctgtcctgca ttcgtgaaca tcccttttac gctaggtata
    18481 tcttcgatcg ctttttcgtc cgcttttcct ggatcattca ggacaaaacg catacgagtc
    18541 gcacaatgac taactgcagc aatattttct tttccgccga tatcatgtaa cagcttctcg
    18601 gcatctgctt gatattttgc catatcaata aacccctttc tcttaacttg tctatgcaag
    18661 ttgtttcttc atttactata ctttttttcg gagagaattg caagcgtttt attaaaaagt
    18721 acgagagaaa atgatatcgt tttaaaaagc ttaaaataag cttttcttaa aaataggaac
    18781 attgaaatac aaaaagagct gtagtatagt gaagaaatga tggaagaacg agtgagggaa
    18841 aaaacatgaa taagttcaat gaaatttttt tagatttgga acagaaaatt ttagcgaaag
    18901 aatatcctcc gcattcgctt ttacccagtg agaaccaatt gatcaaaatt tatggtgttt
    18961 cacgagaaac aattagaaaa gcgctgaatc ttttgactac ggaaggttat attcaaaaaa
    19021 aacaaggaaa aggttcgatc gtactggatc ggaatcgctt cgactttcct atttctggat
    19081 tgactagcta taaagagcta caggagaccc aacgtattcc aagtgaaacg atcatccata
    19141 cgttagaaga aacagaagtg acaaaagcat tgaacgagat cactggctgg gagatcggtg
    19201 cgcctgtttg gcatttgata cgcgaaagaa aaatcaatgg cgaagtagtt atattagaca
    19261 ccgattatct attaaaggaa atcgttccta ttttaactcc agcacaagca caaggatcga
    19321 tctatgaata ttttgaaaat gagctttctt tgacgattgc ttacgcgcaa aaggagatca
    19381 cagtagaaga agtaacggaa atcatgaaaa cagcgatgga tcttcacgat gacacttatg
    19441 cagtagtagt cagaagcctc gttcatctgg aagatacccg ctgtttcgag tattctgagt
    19501 cggttcaccg attagataaa ttccgtttcg ttgagtttgc ccgacgccga aaagtgtaaa
    19561 tagttcaaaa gaaagcaaat tgttggcatc tgtccacttt ggcgctatga taggatcaca
    19621 aagggggcat ttttaatgga taaaccaata agaccagaaa aagaagaact gaaagaaaag
    19681 ttatcaccca tggcttatgc agtcacacaa gaaaatgcaa cagaacgacc atttagcggg
    19741 aaatatgatg atttttacga aaaggggatc tatgtagata tcgtcagcgg tgaaccgtta
    19801 ttcagctctg cagaaaaata tgatgcaggt tgtggctggc catcattcag caaaccaata
    19861 acgaaaagac aagtgcgcga aaaagccgat ttttctcatg gcatgcatcg aatagaagtt
    19921 cgaagcaaag aagcagattc tcatctcgga catgtgttta ccgatggacc agtagatcaa
    19981 ggtggtctac gttactgcat caattctgcc tcactgaaat tcattccata tgaaaagatg
    20041 gatgaattag gttatgggga attcaaatca cttgtagaat aatggaaata cacaaagagc
    20101 ggtacaatac ggggatttac tcgatcgtcc gctctttttt tattcaagcg attgctattt
    20161 gaaagaaacg ctccctagac tccttttttc acaaggtgaa atgtgctaca ataaagggaa
    20221 cgtatgtgtg caaaagaaag aggactagaa tatgtatgaa tatctgacag gtgtggttac
    20281 ctttatcaac ccctattaca tagtagtaga aaccaatggt atcggctacc agatcgcact
    20341 aggaaatcct taccgctaca gtagcaaact aaatcaaaaa ataaaaattt atgtccatca
    20401 ggtaatacgt gaagatgcgc atcttctgta tggatttgat tcgctagaag aaaagcaact
    20461 gtttttgcga ttagtaagtg tttcaggaat cggtccgaaa agtgcccttg caatcatggc
    20521 aagcgaagac cactccggtt tgattcaagc agtagaatcc agtgatgtca cttatttgac
    20581 gaaatttcca ggagtaggga aaaagacagc acaacaaatg atcttggatc taaaaggaaa
    20641 attcggtgag ctgagtatcg atacaccttt tagtctattt gatgaagcaa acacaaaaga
    20701 cgctacagct ctatctgaag caatggaagc tctctcggct cttggttaca gtgatcgaga
    20761 aatcaaacgt gtagaaaaac aattaaaaga agtagataat cagacaacgg atgagtatct
    20821 acgccaagca ctcaagctaa tgatgaaaaa ataaggagga gaccaaatga cagatgaata
    20881 cttgttatca ccagaatctg gcgaaaatga actgagtttg gaaaaatctc ttcgaccaca
    20941 gctgctttct caatatatcg gacagagaaa agtcaaacaa gaacttcaga tttacatcga
    21001 agctgccaga aatcgggaag aacctttaga tcatgttctg ttgtacgggc ctcctggatt
    21061 aggaaaaacg acgatggcca tggtcattgc taatgagatg accgtaaata ttcgtacgac
    21121 gagtggccct gctatcgaac gtccgggtga cttagtagca atcttgaatg aactggaagc
    21181 aggggatgtc ctgtttatcg atgagatcca tcgattacct agagtggtag aagagatgct
    21241 ttattcagct atggaagatt tctatgtaga tatcatggta ggacaagggc caaccgcaca
    21301 tccggttcat ttccctcttc ctccctttac tttgattggc gcgacgacac gggcaggtat
    21361 gctttctgca cctttacgag atcgatttgg catcatttct catatggaat attatgacga
    21421 acaggatctg caggagatcg ttttacgttc ttcggatatt tttcaaacag agatcattga
    21481 agaaggggca ttggaaattg ctcgccgatc acgaggaacg cctagaatcg ctaatcgctt
    21541 gctcaaacgg gttcgtgatt ttgcgcaagt ccaaggagac gggaaaatcg atcgtcctat
    21601 agcggatcag gcacttactt tgctacaggt cgatcaggct ggattggatt atgttgacca
    21661 gaaattgttg cgaacaatga tcgagttata tggcggcgga ccagtagggt taagtacgct
    21721 ttcagtaaac ataggagaag agcgtgagac ggtagaagat atgtatgaac cgtatctgat
    21781 ccaaaaagga tttttaaagc gtacacctag agggcgaatt gctactgctt atgcctatga
    21841 acattttggt tacgactatc atatatgatt tttgctttta gattggagga aaatgaatga
    21901 gtgatacaac acgtacgaaa caattgatca aaaacgtatt gattgatttg tgccaaacgc
    21961 aatcttttcg aaaaatcagc gtccaaaaaa tcgcaaacga agcaaaaatc aatcgacaaa
    22021 cattttatta tcattttaca gataaatacg atttgcttcg ttgggtctat tgtgaggatg
    22081 cattgcatta tttagatcca caatgtcttt cacttgataa ttgggaagag caagcattga
    22141 aaatgctgaa agcaattgct gagaagaaag aattttatgc aaatactgtg ctttctgacc
    22201 cagaaatttt acaaaaagag ttttctcaac tgatccaaaa atcatttatc aagatttttg
    22261 aacaaatgga tgaagaaaga caactttctg caagagacaa actattttat gctcgttttt
    22321 tttcttacgg ttgcagtggt gtattagtca attggatcac gaatgaatat gaagaaacac
    22381 cgatggaaat cgctatgcag cttttccgtt tagccaagga tacggaatta tattcttatc
    22441 gtctttatgc aaaagaagag ttggaagaac agtaatagaa aaattttttg ttggaaaagc
    22501 aataatcgct aaaaggagat ggggaaatga caaaaatttt atttgtttgt ttgggaaata
    22561 tttgccgttc accgatggca gaaggcttgc tgagaaaacg aatagcatta gaaggaaggg
    22621 aagcagaatt ttttgtggat tctgctgcta caagcacgta tgaagttgga aagacaccgc
    22681 accctggaac gaaaaaaata ttaaaccaag aaaaagtaga tatgacgaat atggttgctc
    22741 ggcaaatcac accccacgat tttgaaactt ttgattggat cattggcatg gatcaagaaa
    22801 atgtagaaga actgaaacga agagcaccac gctcagcgca ggggaaaatc catttgtttt
    22861 taagtagcat tcctggaaaa gaaaaagaaa atgttcccga cccatactat accaataact
    22921 ttgatgagac atatcacttg atcacagaag gcctgaatca ttggtggaat atttggattg
    22981 atgaaaataa aataacaaac aatttgtaaa gttaacgctt tctttttaag gcgatgttag
    23041 agcatttcct tgtttttttg taaaatgtta ggtaaaataa aattaattaa gtaacacttt
    23101 cttagctaaa ggaggagtgc attcgatgaa cgaagagaca gtatcgccaa tgaagaagct
    23161 tacgaagtta gttactcata cttcgtatca actttttgat tcttttgtaa atgacactga
    23221 agaacatgct aagaatgtac gattgcaagg aaagtttatt cttgagctgc aaaaagctgc
    23281 tactgaacat agtctagtta tcttacaagt aagagcaaat cgtttgagta gcaagtatga
    23341 aacgatctca ggctgggtgg cgacaaaaac ggtcgaaaaa gaccgagtga tgatccgcct
    23401 acaaaacgaa gagcagcaga tccggatggt tcctgtagaa caaattgaaa aaataacaat
    23461 cctttcacca gcaggggaac aggaacggtt atcaaaataa tttataccag atagaaaagc
    23521 acgtatgagc ttgtaccatc aatcattact aatcaaaaat aaccgaatgc tttaagctga
    23581 tatcagtaaa acggaacgtt ttaggatgag tatcacagca tgacagcatt gggttgatta
    23641 gttgaatcga ttggatggta caagtttttt tgataaaaaa aggtcctgct agccggggag
    23701 ctagcaggat aaggagttaa aaatgaaaaa gtgttgttag ggttgtttgt tggtatgctt
    23761 atataatagt cactagttgt gaatattttg tgacgaaagt tttcgaaagt tgttaagttt
    23821 ttttgcgaaa actattttta tatcatatat atgtctaaaa tctatattat aaaagaaatg
    23881 ttgtttgatc agcttctctt cggtattagt tcaaaacaaa acggcaaagt atatggagct
    23941 actttgccgt tttgtttctc taatactcag gtcagtttat agacgctcaa taaacggtgt
    24001 tcaaaagcca gtttctcggt attaagtcaa atttatgcaa aaatgtgaga agccattttc
    24061 aaaaaatatt tcttaatact cgaaactgag cggctttttc acgacctctc aataaacagt
    24121 gttcaagaga cagcctatcg gcaataaggc tgaacgtctc tttcacaacc tcttttatca
    24181 atctgctact tcttctttct tagcgaaact ccaaatcagt ccgataacaa aaccgataac
    24241 tgctggtacg atccacccca tccccaattt gaaaaacggc aagtagcttt ccgcaacata
    24301 taaaagattt tgaacaatag gtgtttgttt gatagtttct gggcaagcgt tcaacccatc
    24361 aaaaatcgaa gcaatcaatg taaaataagt agtcattcga taaacagccg tacgctgctt
    24421 aaacaaagga ccaactaaca ctagtaaaat cagtgtcatt gctaatggat agatgaacat
    24481 caaaacaggc aacgaaaatt gaatgatatt cgtcaagcct acgtttgccg ctaaacaagg
    24541 aaggatacta gcaagtgcaa caaagaatgc gtaatttctt tgcggaaaca aatctgtgaa
    24601 tgtttctgca aaagctgtaa tcaaaccaat accggtcttc aagcaagcta atataacgat
    24661 aaatgctaat aagatactgc cataagtacc taaataatga tcagcaatct gagccagcgt
    24721 gatcccgcca ttttcactta gagcaaagct acctaagctc atagcaccta aataagcgag
    24781 caaagtataa atgattccca tcaatataat gctgatcgca ccagatttga tcgtgtcttt
    24841 agcgatttca gcaggttttg ttacacccat gttttggatt gttgtgacaa tgatgatccc
    24901 aaatgctaaa gcagcaagcg catctaatgt gttatatcct tgagtaaacc ctgtgaaaaa
    24961 agcatgctct tgataatttg gctgaatagg cgcttgattt acagaaccta gtggattcaa
    25021 aaaagcaagg agaagcaaag cacctaataa aaccaaaaat gcaggattca ggaactttcc
    25081 aacataatct aatatctttg tcggttttct tgaaaaccac caagccagca caaaaaatac
    25141 aatactgaaa attgctagat atagcgtctg tctgctagga tcgataaatg gtgctaaacc
    25201 aatctcgaaa gatgtggttg caaggcgagg taaagcaaaa aacggtccga taactaagta
    25261 aagcaagatg gtaaaaacca tcgcatatgt ttgatttatc cgttttgcta attcatatac
    25321 acctgagctt ttcgatacac caatggcaat cacccctaag aagggaagac cgataccggt
    25381 aaccaagaaa cctagattcg cccaaaaggt agcagcgcct gcttcttgac ccaaatgaac
    25441 gggaaagata agatttccag caccaaaaaa tagaccgaaa agcatggagc ctataaaaag
    25501 atagtcttta aatgataatt tttttgtcat acataactcc tttacggttt ttttaaaagt
    25561 aacaaaattt cgaaaaatat gcaacaattt tcagaaaatt tcctatataa aacgacggtt
    25621 atttaataac gtaagtgata aaataaaagc gctgaatcat ctgaggagga atcatcgtga
    25681 caaagtcgag attagaagca tttactgatg gagtggtagc cattgttttg agagttcttg
    25741 tattagagat caagatccct aatacatcta gctttcattc tttatgggtc atccgaaata
    25801 cattattagc ttataccatc agctttatat ttgttgcggt gatctgggtg aaccatcatc
    25861 gtatatttca gatggcagaa cgaattaatt accgagtggt ctggtcgaat atctttttgc
    25921 tcttttggct gactttatgt ccttctgtta ctagttgggt cgggcgtaac cctgaagctt
    25981 tttggcctga gatggcttat gtcttagtat acacgatgtg gagtttttct tttggtatct
    26041 tgatgaggca aatcatgaaa gcaaataaac cagattccca tgtcgtgaga gttttgtcaa
    26101 aggaccgtcg aagtcttatc tccatgatga ttaacttatg cttgattgcc ggtgtctttt
    26161 ttgtaccgtt gatcgggtta ttcggacgtt tttttgtatc tggtatttgg attttttcct
    26221 ataaaaaagc agatgcctat tatcagcggt tgtttccggg gaaatagaaa agaggctggg
    26281 acagaagctt ttaactccaa gaaataagcc gaaatttcac aaaaatttaa gaagcaattt
    26341 tcgtaaattc cttcttattt ctcgaagctg accacttctg tcacaactac tttcttactt
    26401 cttacgttca tatttcatat gatagattag taatagaagc aagaaccaca caggtgttag
    26461 taataaagcg attctcgtat cttttcctag gaacaagacg acacacacaa aggccatgta
    26521 aagaaatacc agataattac taaatgggta caacggcatt ttaaatgaat gagctttttt
    26581 gccgaggata cttttacgat atttcatgtg cgtgtagaca agcatactcc agataaacaa
    26641 gaaacaagta gtggcaatac tgcttactag tgtgaatact tcactaggca tcacgtaatt
    26701 caagataaca gaaatgaaaa tgacgatagt agaaaatgtc aaagcattcc ctggcacatg
    26761 atgtgttgtt aactttttga attttttagg ggcactgcct tcttgcgcta aagaacgaag
    26821 catacgtcct gtactatata ttgcactgtt gcaggcagaa gctgctgcac ttaatacgac
    26881 taaattgatc aaggaagcag caatcgtgat cccaatttcc gaaaatactt ctacaaatgg
    26941 actgttatct ggattcaagc tgttccatgg atagattgcc ataatgacaa aaagagatcc
    27001 taagtaaaat aagatgatcc tgataggaat attattgata gcttccggca acactttttc
    27061 aggattttct gtttctccag cgactaatcc aactaattct atcccagcaa aagcaaaggt
    27121 taccatttgg aaagctaaga taaatccttt tgcaccagtt ggaaagaatc cgccatgaga
    27181 ccatagattc gtgatactgg ctgtgccaat atctgtcgta tagtgggtga ggatcatata
    27241 agctcctacg aggataaagg caatgatcgc tacaactttg atcaatgcaa accaaaactc
    27301 taactcacca aacaatgaga cagccaccat attcaagcaa agaagaagac ctaatgcaat
    27361 cagttcagga agccactgag gaacaccagg cgcccagtag cgtacataca ttccgatggc
    27421 tgttaaatcg gccattgcaa tcgataccca gcagaaccaa tacgtccagc cagtgatgaa
    27481 agccgctttt ttgccaagat attctgcaac aaaatccaga aaagaatggt tattggtgtt
    27541 tgataacaga agttcgccta acgcacgcat aatgaaaaaa cagatgcatc cagtaataag
    27601 ataggcgagc aatatcgaag ggccagctag ctggatagac tttccggaac ctaaaaacaa
    27661 ccccgttcca atcgctccgc caatagaaat cagttggaca tgtctatttt tcagtccacg
    27721 ctgcagttct tgctcttcat tcatttttaa atcatcctaa ctaaaaaagt ttaattactt
    27781 ccatattaaa agagttttct gaaaaacaca actaagattc caaaattttc agaaatttac
    27841 cttttatgag taattcataa aaaagaactg cggtaaacac cgcagctctt tgtcccaatc
    27901 agctattgat cctaagcatt taaaatacgt gaaaggaatt cttttgtacg ttcttctttc
    27961 ggatggacaa agatatcttc tggtgtacct tcttctgcaa tcacaccttt atccataaaa
    28021 atgacacggt cagatacatc ttttgcaaat tccatctcat gcgtcacgat aagcattgtc
    28081 aagccagtat gtgctaagtc tttgatcgta ttaagcactt cgccaaccat ttcagggtct
    28141 aacgccgaag taggttcgtc gaatagcaat acatctggat tcattgataa tgcccgagca
    28201 attgcgacac gctgtttctg tccaccagat aattgtgctg gtttggcttc gatataacga
    28261 tccataccaa ctttttccag attttcgatt gctacttttt tggcagtttc tttatctcgt
    28321 ttcaggacag ttgtttgacc agacatacag ttttccaaga cattcatatt gttgaacagg
    28381 ttgaaggatt ggaaaaccat acctagattc gttcgatatt taggtaaatt atatttagga
    28441 gctaatacat tttctccatt ataaatgatc tcgccgcctg ttggtttttc cagtaaattg
    28501 atacagcgca agaaagtaga tttaccagaa ccggaagaac cgatgatcgt cacaacttca
    28561 cctttattga ctgttacgct gatatctttc aatacttcat tttcaccgaa gctttttctt
    28621 agatgtttga tttcaataat tttttcattc atgattcttc ctccttattc ctgcaaattt
    28681 gaattgtcga tttcttcaag cttggtataa gctgaaggac catcgatttt cttttctacc
    28741 gcccgcagga ttctggtgat cgtaaaggtc atgactaagt agatgatccc gacgatcgta
    28801 aatgtttgga agaattggaa attcgcaccg gaagcagagt ttccttggaa gaacaaatca
    28861 gctacgccga ttacacttaa aactgctgta tctttgatat tgataacaaa ctcatttcct
    28921 gttgctggca agatattccg taaaacttgt ggaataacaa cttttctcat cgtttggcca
    28981 tgggtcatac caatggcttg cgcagcttcg aactgccctt catctactgc aaaaatcccc
    29041 ccacgaacga tctctgtcat gtaagctcca gtattgagag agacaataaa caaagcggca
    29101 atcgttcggt tcaatgaaat gccaaaagcg agtgccagac cgtagaagat gaccattgct
    29161 tgtaccatca tcggtgttcc gcggaatact tcgacataga tagaaagaag tgcatttccg
    29221 attttttgga agaagcgagt cagtgcattt cctgattcag gtaccgaacg gaagacacca
    29281 atcagtaatc caagtgtagt accgacaact gtcccgatca atgcgatgaa caaggtaagt
    29341 ccagcacctc gtaagaacat attgccgtat tgtttccaaa tcgtagtgaa atcagaaatg
    29401 atgccaggtt tttcttcact atcagttgca gcaggctgat ctttgactgc ttggtccata
    29461 attttggttc gttcatcgtt agagatgcca gaaagaatct ggtttacttt ttggatatcc
    29521 gggtcgcctt tacgcatacc aacagaaatc tgaacatctt ccgggtttgt ctggaagcca
    29581 tctgcaccgc tgaattcaag catttccaag tctttgttca cactagttgc cgttacacct
    29641 tccggacgtt cgctgacata accatcgatc attccagaag ctaaagctgt tctcattgct
    29701 gagaaattat ccattgcttg ttgtttctgc acatctggta tctgatcgat cacagtatag
    29761 tggaatgtat ttaactgagc agtaatcttt gctccagaaa gatctgctaa actttttgct
    29821 tttgcatatt tgctatcttt ttgaactact actactaatt gtgattcgta ataaggatcg
    29881 gtaaaatcaa tttcttttcg tcgttcagca gtagggctca ttccggcgat gatcgcgtcg
    29941 atcttgcctg attgaagggc aggagccaga ccatcccatt tcgtttggac gatgactaat
    30001 tttttcccta aaccatctgc tactttttta gcgatttgaa catcataacc gccggcgtaa
    30061 ctattttggc cttgaattgg caaggcatta ttctcatctg tttgttgaga ccagttgaag
    30121 ggtgcatacc cagcctccat acctacgcga aactcatcgc tcggtgtttt ttcatctgca
    30181 tttgccagag atcccacact gaatagtgag aagacaaaag caaatagtac ggcaactgaa
    30241 accatatttt ttttcatttt tttctccctt tctgattata tgatacacga gcggtctaga
    30301 tgaaatgcgg taaagcttta tctgaaaaaa taaaaagcga ccgatttata gatataaaaa
    30361 ggccgctaaa attccataag gtgataaacc tctcacaaaa catagcgcaa cttagcatca
    30421 ctgctaagac agtccgcaag ctcttaactt gcgtcccaac atacactcca agcaaaaaag
    30481 tgcacatttc ggcgatcctc cctattcctg cttctccatg tttgcttcac tccacagggt
    30541 taatgattct cgcgacctct acctcattga ttgaggcatc tcgtattcag ttatttatac
    30601 aaaaaaagtt tacgatactc gagaggcttt gtcaatctat aactgctgtt ttttgaaaaa
    30661 atactgataa atatagaaat ctttactttt accgaaaaaa caatcaaaat gtgaatcatc
    30721 tatccattgc aaacgatagt ataattatac acaaaaatgt atgtaataca ataaaaaaag
    30781 aactagaaca gaaaatttcc tgtgtccagt tccatctcga tcatttaagc tgccgctttc
    30841 tttggctcac gtcctaggat cgcttgcaaa agaacaacga cgccataaat ggctaatatt
    30901 agataaatca aacttgagta ttgggtgatt ccccagcccc atgttcgata tacggctaat
    30961 gtaatggcta aaacaattgc ggcaatcacg ttgaataaag caaaagccca aagatttcca
    31021 ttttctttgc gtacaagata aaaaacagag aaataaacgc ttaacgctaa ccaggcgata
    31081 caagccagaa taacgcccca ataaataaag aatggtaaca tatcgattca cctcactttt
    31141 atacagcagt attgtaacac gttttcattc ttttgtcagt gaacattgtg ataaattgtt
    31201 caaatgccca taacactttt ttattcccat ttttctgact tttcaacaat aaatttgttc
    31261 gagtatgggc tataatggaa agtgaggtga caaaacatga aacaattaac agtagacgaa
    31321 gcagcaaaag agatattagc ttatcagcaa attacatttt tgaccggtgc aggggtatcc
    31381 acaccttctg gagtgccaga ctatcgatct ttgtcaggaa tctaccacgg gatggagcag
    31441 ccggaatact tgctgagtca tcaagcaatg gtgaatgaac cggataaatt ctatcaattt
    31501 gtcaaaaaga tttatcatcc tgaagcaaaa cccaatatca ttcatttgga aatggcacat
    31561 ctagcgaaag aaaaaaatgt ttgggttgtt tcccaaaaca tcgacggttt gcacgcaaag
    31621 gctaaaagcc cgcaactcgt caattttcat ggaagtttgt atcaatgtca ttgtcgaaaa
    31681 tgtcaacaaa cggtagactg gaaagaatat cttcacagcg acaaacatca agcatgcgga
    31741 ggacaaatac gcccagacat cgtgttatat ggtgaagggt ttcaagatgg agtcatggag
    31801 caggcggctt atgccgtcag ccaagcagag ctgatcgtca ttgttggaac gagttttcaa
    31861 gttcatccgt tttgtgatct ggtacagttt cgccagccgt cagccaaaat cttagtaatc
    31921 aatcaaacgc ctgtttattt agcggagcct tatacgttta ttcaagaaga cggagcggtt
    31981 gttttcaaaa aaatacaaga attaggagca gattatgact gaattcaaaa ccgaaaaaga
    32041 aaaaatgatt gcaggcgagc tttattttgc caatgatcct gagctggttg ccgaccgtat
    32101 gtttgcaaga agtcaaagcc aaatcatcaa ccaagcagaa tcagcggaac tgcgatccca
    32161 attgttgaaa gagacttttg gccgtacagg gaaaaaaatc tatatggagc cagttatcaa
    32221 ttttgattac ggatataata tatttgttgg ggagaatttt tatgcaaatt tcaattgtac
    32281 atttttagat gtcagcacga ttgaaatcgg tgataactgc atgttcgctc cgaatgtcca
    32341 gctttacacg gcgacgcatc cactacatcc agtgaaacgt aacagcggtc tggagtacgc
    32401 aaaacccatt aaaatcggta ataatgtttg gttaggcgga ggagttatcg tcacgccggg
    32461 tgtcactcta ggggacaacg tagtagtcgg cgcaggttcg gttgtgacca aatcttttcc
    32521 cgacaatgta gtgattgcag gaaatcctgc tcgtatcatc aagacagtag aggaagaact
    32581 aacagaagag tctcttgaaa cattacgtca taagatcgat atgatcgatc gagagttgat
    32641 tgctttatta gaaaaaagaa tggatacggt aactaaaata ggacaagtga aaaaagaaga
    32701 aaaccaagca gtttatgatg caaaaagaga gcagcaagta ttggataaag ttgttagtct
    32761 gctgaaaaat aagggatata aggaaacaat aacagatacg tatgttgatt tgatgaaaca
    32821 ctcgcgcgaa taccaaaaca aaatgaagga ggaataagtg tgatcgaagg agagacacgg
    32881 cgaaaacaaa tcatgcaaac cttggcaaat acagaaaaac cagtgagtgc tagcaagttt
    32941 gcgaaaactt ttggagtcag ccgtcagatc attgtaggcg atattgcttt actacgagct
    33001 gcaggtaaaa gtatcgtggc aacagcaaga ggttatatcc tcgaaacaga agaaaccaat
    33061 actgggcaca tcagtaaaat cgctgtccag catggaaaag atcaaacaga agaagaactg
    33121 agactaatcg ttgaaaatgg tggtgaaatc attgatgtaa ttgtggaaca tcccctttac
    33181 ggtgagttag caggaacttt gcacatcaaa acgccacaag atatcgattc ctttatgaga
    33241 cgttatcaaa aaagtaatgc tactttgtta tccgaattaa caggcggcat ccatctgcat
    33301 acgatccgct acccagaaaa gcatatgctg aaaaacatca aacaaaaact agctgaagca
    33361 ggaatcctct acgaaggata acatcgtagc tgctactttt tatcctagaa actattctat
    33421 catctctgag ataaacaccc atatggaaga aataagaaaa aataagaaaa gatgcgtatc
    33481 caatacacag aattttttgg aattgcgatt tctagatgtc tatctaaatt tcgattagtg
    33541 gagaaacata aaaaagggaa gtgcagcttt tgagcactcc ccttttttat aaagctttct
    33601 tgtagcatag tatcgaagga acctgccaat tctctactcg atcaccaata cgccttcttt
    33661 tagtgcaaat ggattttgat cgttgatgtg gtcatagaac atcacaccgt tgatatggtc
    33721 gatttcatgt tgaacgacga tcgattcata attcttcaaa cgaatcttgt gtttttcacc
    33781 attcatatca taataagaca cagtgatttt ggcatggcga acaacatatc ctggtacttc
    33841 tcgatcaaca gataaacagc cttcaccttc ccctaaacaa gcatcttgaa cagaatggct
    33901 caaaatttta ggattataca taacagtact tagagaaggt ccatctgcct ctgggtcagg
    33961 acttggtacg tgaacagcaa tgattctttt agaaatatct aattgaggag cagctaagcc
    34021 aacaccgcct cgtaagtgta gttcttcagc tttgattgga tcttggctgt tttttaagaa
    34081 ctcaagcatc tcttttccta aagagatatc ttcttcgctt aatggaagag aaacttcttt
    34141 tgcaacttcg cgtaaggtcg gatttccttc acgaatgata tcatccattg taatcatgga
    34201 atatgtaccc ctttcttaaa agactgtgtt tcaagtctat tttcgcatta tagtttacca
    34261 caaaaatgga aaagtagtag taagattgtt tgaaaactac cttttcttaa tgattaacta
    34321 cttgattttc aacccggttt caggtaaagt atagctgtgt gctctaggca catgacctgc
    34381 cacttggtta agcgaatcac caacgtttta ctcagtcgca ggggaataat cgaaagaggt
    34441 gaaagaaatg gcaatttcaa aagaacgcaa gaatgaaatc atcaacgaat acgctcgcca
    34501 tgaaggggat actggttctc cagaagtaca aatcgctgta ttaactgaag aaatcaacca
    34561 cttgaatgaa catgcacgcg tgcacaaaaa agaccatcat tcataccgcg gcctaatgaa
    34621 aaaagttggt caccgccgta acttgttagc ttacttacgt aaaactgacg ttcaacgtta
    34681 ccgcgaattg atcaaacgtt taggcttgcg tcgttaattt tagtcaaaaa gagtgggatt
    34741 cacatgaatc tcactttttt tactcgtata tcaattaagt aaaattccgt gatcaagcta
    34801 gatggactcc tactaaccaa atgaaagctg ctcaaaagac ggctttcggc agttttcatt
    34861 tgtttagtag cgtcttgctt tcacgagaag gaaaaggaga agttatgtca gaaaaacaag
    34921 tattcaaaac gacatggggc ggccgtccct tacaaatcga agtcggtcag ctagcaaaac
    34981 aagcaaacgg agccgtttta gtccgttatg gagatacagt agtcttaagt gcagctgtgg
    35041 cctctaaaga agcaaaagat acagactttt tcccattaac gatcaattat gaagaaaaaa
    35101 tgtatgcagc agggaaaatc cctggaggtt tcatcaaacg agaaggccgt ccaagtacgg
    35161 aagcaacatt gactgcccgt ttgatcgacc gtccaattcg cccgatgttt gcagaaggct
    35221 tccgtaatga agtccaagta acgaacatcg tgatgagtgt agaaacagac tgctcgccag
    35281 ctatggcagc aatgctaggt tcttctttag cactatcaat ctcagatatc ccatttgacg
    35341 gcccaatcgc aggcgtagaa gttggtcgtg taaatggcga gtacgtcttg aatccaacag
    35401 tagaacaagc agaacaaaca gatatcgaat tgactgttgc cggaacgaaa caagccatca
    35461 atatggttga aagcggcgcg aaagaagttt cagaagaaga catgcttggt gcattgctgt
    35521 ttggttttga cgctatcaaa gagttagttg ctttccaaga agaaatcgtt caagcagtag
    35581 ggaaagaaaa aatggaagtg actttactgc aagttgatga agtattgaaa aaagaaatct
    35641 ttgatgcttc ttacgctaca atgaaagcag cagtaatgac tgaagaaaaa cttgctcgcg
    35701 aagataatat cgagcaggtg aagatcgata tccgcgaagc ttatgctgag aaatttgcag
    35761 gtcatgaaga tgaagatcac ttgttgaaag aagttaaaca aatcacagaa gatcttgaaa
    35821 aagatgtcgt acgtgaattg atcaccatcg ataagatccg tcctgacggg cgtaaattgg
    35881 atgaaatccg tccattgtca tcagaggtca gcttattgcc acgtgtacat ggatcaggtt
    35941 tgttcacacg aggacaaaca caagctttat cagcttgtac actcgcacct ctaggagagc
    36001 accaaatcat tgatggttta ggggtggaag tcagcaaacg attcatccac cactacaatt
    36061 tccctcaatt ttctgttggt tctacaggac gtgcaggctc tccagggcgt cgggaaatcg
    36121 gacatggcgc attaggtgaa cgtgcgttag cacaagtaat cccttcagaa gaagaatttc
    36181 cttatacgat ccgtttagta gcagaagtat tggaatcaaa tggttcttct tcccaagcaa
    36241 gtatctgtgc tggcacatta gcattgatgg atgcaggtgt accaatcaaa gcgccagtcg
    36301 ccggtattgc gatgggactt gtttctgatg gtgaaaacta tacgatccta actgatatcc
    36361 aagggctgga agatcatctt ggcgatatgg actttaaagt tgccggtaca aaagatggta
    36421 tcactgcttt acagatggat atcaagattc aaggaatcac agaacaaatc ctaactgaag
    36481 cattaactca agcaaaacaa gcacgtatgg aaatcttaga agaattgact tcaacgattg
    36541 ctgctccacg tgaagaatta agccaatatg caccaaaaat cgaaatgatc cagatcgaac
    36601 ctgctaaaat caaagatgtt atcggtaaag gcggcgatac gatcaacgga atcatcgatg
    36661 aaactggtgt gaaaatcgat atcgatcaag atggaaaagt aagtattgct tcagctgatg
    36721 cagaaatgat caaaaaagct atcaagatca tcgaagattt gaccaaagaa gtcaaagttg
    36781 gtgaagttta tctagggaaa gttgtccgta tcgaaaaatt cggtgcattc gtcaacttga
    36841 tcaaaggaaa agatggactt gtccatattt cccaattagc aaacgatcgt gtcaataaag
    36901 tcgaagatgt tgttaaacta ggtgatgaga tccttgtgaa agtaactgaa atcgataaac
    36961 aaggacgcgt aaatctgtca agaaaagcaa tgttgaacga agatggttca acgaaaaatg
    37021 aagaaaaata atttaattca taggaataat gtacttgtga tcgtgaaggt gacaggggga
    37081 caataattat tcgaaaaaag gggactgtga caaaagccgt gtcacagtcc ttttttttcg
    37141 aataaacggt gttcaaaagc ttgcatctgc ggtaataagt ccagccaagt taaaaacatt
    37201 aggagctgtt tttatttggc tgttcttatt acttgatgca gaacagcttt tttacaacct
    37261 ctccaataaa cgatgttcaa tcagctgtcc ctcgatatta gctcaaaaaa cggcaaaata
    37321 tgaggaacta tttttccgtt tgtttctcta atactcaggt caaaccgctg atttcacaac
    37381 ctctgattca cggtgttcca gaagcagctt ctacggaaat aagctgaaca cttttgttac
    37441 aacttctttt tttctggagt ttcttggcgc atatttttgg agtccttctg aataaagcgt
    37501 aaaataaaag tgagaaatat ggataaagga gggaaaggag atgctgaatt ttttatggtc
    37561 gttatttatc ggcggtatat taggtttgat agctggcgca attttaggaa aggaagtccc
    37621 gggaggcgta tctggaaata tcattatcgg ttttttaggt agttggctcg gcgaattttt
    37681 actaggacca ctgggaccgg aactcggcgg tttttatttg ataccagcat tattgggtgc
    37741 aattttttgc cttgctcttt attcatttgt ggcaagacgt atcagagaac gtccaaagaa
    37801 agaataattt tattcaaata agttgacact tcagtgtgtt acttggataa ttagaagcaa
    37861 tttaggaaag cgggtgtcat ggataagaat atcatctatc attcaagctt acctaaagga
    37921 tatccatata acaagaaatg agggaaaaac gtggcaacaa cgcacaatac caaaaagata
    37981 tcaatgactg gcctgcttgt agcgatggga gtcgtctacg gagacatcgg gacaagtccg
    38041 ctttacgtta tgaaatcgat tgttgaagat aatggcggat tgtcaggaat caatcctgat
    38101 tttgttatcg gatcagtctc attgattttt tggacattga ctctactgac aacaatcaaa
    38161 tatgtcgtga ttgcattgaa tgcagataac catggtgaag gtggaatttt ctcactctac
    38221 acacttgtcc gaaaaggtgg gaaatatttg atcattcctg caatgatcgg aggagctgcc
    38281 ttactagcag atggggtatt gacccctgct gtgacggtaa cgactgcgat tgaaggcttg
    38341 cgaggaattc cgcagttttt tgaccggttc ggtaataacc agaacgtgat cgtcattatc
    38401 actttagtta ttatttttgt tttattcatg gtccaacgct ttggtacaga tattgtcgga
    38461 aaagcattcg gaccaatcat gttcgcttgg tttacttttt taggcgtaat gggattaatg
    38521 aacttcagtc aagactggac agttatccgt gcactgaatc cttattatgc tatccagctg
    38581 ctttttagtg cagataacaa attaggcttg ttcattcttg gaaatatttt cctggctacg
    38641 acaggtgcag aggcgcttta ttccgatctt ggacatgtcg gcaaacgaaa tattcgtctg
    38701 agctggcctt acgtcaaaat ttgtttggtt atcaattatt ttgggcaggc tgcgtggttg
    38761 ttgaatgttt atcaaaaccc tgaagcacag caaatcaaga acctgaatcc attcttccaa
    38821 atgatgcctc aaagttggat cgtgatcggc gttgcttttg caacagtcgc tgcagtcatt
    38881 gcttcgcaag cattgattac aggttcgttt acgctagttt cagaagcaat caagttgaaa
    38941 ctgctgcctc ggttaaagat catgtatcct gggaacagta tcgggcaaat gtatatccca
    39001 gcagtcaatc tgattttatg gatcgcctgt tctctagtgg ttgtcacatt ccgaacgtct
    39061 catcatatgg aagctgctta cggtctatcg atcactgtga cgatgttgat gacaacagca
    39121 ctgctttact tttatcttat ccaaaatggg cattctcgtt ggcttgcata tttagttacc
    39181 tttttcttcg gtgcgataga agtcgttttc tttatctcaa gtgttgtcaa attcttccga
    39241 ggcggttttg tagccgttct gattggtttg atcatcttag tcgtgatgtt tatttgggaa
    39301 caagggaaca ttattcgcga atcgtcaacc gaggatgtgt ctttgagaga ttatatcccg
    39361 cagcttagag aattacgtga tgatacttct ttaccaatct atcagacaaa tgtggtcttc
    39421 ttggttcctg atatggctga agataaagtt ggccgacagt tcatctattc gattttagat
    39481 aaacggccta aaagagcacg agtctattgg tttgttcatg tggaagtgac ggatgagcca
    39541 tatacgaaag aatatcaagt agatatgatg gacactgatt ttattgtcca agtgaatctg
    39601 ttcttaggtt tcagggtcca acaggaaatc aacgtatatg ttcgccaagt catccatgat
    39661 ttgatgaaac aaggccgttt gccaaaacag ccacaaagat actctctgac accagggcga
    39721 gaagttggcg atttccaatt tgtcctgatt gatgaagtag tttccaatgt gacaactcta
    39781 ggaaaatggc aacgtcaaat catgcaggca aaactggcaa tcaaaaaaat cgctacctcg
    39841 ccagaaacat ggtttgggct agagtacagc gaagtaaaac atgaaactgt tccgcttatc
    39901 attggaaata ctcggaaaac atggttgaaa gagcggttgt gaaagtagcg ctttgacctg
    39961 aactaatgtc gaatgtcagc tacttgaaca ccgtttatca gaggtcatga aaaagctgta
    40021 ctgcaacaag cagtaagaac gagcagataa aaacagcttc tcatgttttt catctgtttg
    40081 ggcttaatgc cgtagatgca agcttttgaa taccgttttt atcagaggtc atgaaaaagc
    40141 tgtttagcaa caagtaacgc aatgattttg aagtgataag gagatgtggc tgaaaaatgc
    40201 cgcatctcct tttttattcc taaatatgaa tacgcattat tttcaatctt tttgtagaaa
    40261 aagggtacaa tagaaaaaga gaaaggcggg agaatatggt tatcaatttc attattttat
    40321 ttttgatgat tgcaattact tcattttttg tagcaagtga atttgcttta gtgaaaatca
    40381 gacgatcaag attggaacaa ctagaaaagg aaaatgtaaa gaacgccaag ctagctcttc
    40441 atgttacaca ccatttagat aactatttat cagctagtca attaggaatt acgctaaccg
    40501 gtttgatcat cgggtgggtc ggtgaaggat ctgtcgcggc tttactagaa ccagtgatcg
    40561 gtaaactacc ttttagttcg gctattagca gtacaatctc tgtggcgctg ggatttattt
    40621 tagttacgta tatcgatgtg gttattggcg aactgcttcc taagagctat agtatcgtca
    40681 atacggagaa ggtcgtcttg ttcgtagtta aaccacttca ttatttttat aaagtaatgt
    40741 ttccttttat ttgggtattg aatcattcag ctgcagggct agggaagcta ttaggtgtgc
    40801 gtctcgtatc tgaaggagaa gagacattgt ctcaagaaga gctgacatta gtcgcattga
    40861 attcttatga aaaaggagag ttgaccaaag aagaatatta ttatttggaa aacgtgtttg
    40921 aatttgatga taccttggca aaagatatac aagtagaccg tacgtccatg caagtattcg
    40981 aagcttcaga aaccgtcaaa gaagcgataa aagcttcgat tgaacaagga catactcgct
    41041 atccggttat cttagaatca aaagacaatg ttttaggtta tgtcaccttg ccagatttga
    41101 tcaagcaatc ttataaagat gaccagctga cagtagaaca gttgattgaa gagccgatcg
    41161 taacaactga aacgatccct ataaaaaaat tattgacgat catgcgaaaa aagggtaagc
    41221 atattgctat cttaaaagat gaatacggag ggacaagcgg cttagtgacg attgaagata
    41281 ttttagaaga aatcgtcggg gaaatccgag atgagacaga tttggacgaa gcattgatag
    41341 cggaacagtc agatggctct tatatcatct ccggcaaact aacattagat gatttccagc
    41401 gttattttca tgtagagatt ccagagtttg aagaaacgaa ttttactaca cttgcgggct
    41461 ttgcttctag ccgatataaa gaaataaaag caggaacaat cattgagatt gcatcattcc
    41521 gtttcacggt attagaatac cagcatgcac atatcgatta tttcaaagta gagtccacgg
    41581 aaagaaaaac agaataaaag aaaaaaggct gtgacatttt ttgtcacagc ctttttgtta
    41641 tctaaaaaac ctgttttcgg taaacgaaga agatattttc aataaaagtt ttcattttta
    41701 gccgcaacga tttcttttaa tcaaggctat cttgcttctg catgttctaa tgtgaaatgt
    41761 tcctcttcac ccatgtgttt caattgtttc atcgaacgca tataagagta aagcaacacg
    41821 gcaacagaac cgatccaagc taaaggagaa gctgctgctg ctcctgggta tccccacatt
    41881 cctgtaagaa cgatagctgc aaaagaacgc ataagcaatt ccatcatacc ggcgatcgta
    41941 gggatcttgc tttgtccaag cccttgaagt gtgtagcgta aaatgaacag gactgctaag
    42001 atccagtaaa gagacccatt gatattgaag taggtttgtg ctaattcaaa tacacgtgtt
    42061 tctgatgggt taacaaacaa ttgaacaaag aaatgaccga agaaaatgac gatagcacct
    42121 gctaagagac taaatccgac actcatcagt aaagtttgac gtaccccttg caagattcgg
    42181 ccatattctt tcgcaccata gttttgtgca gtgaaagttg ccattgcaat accaaatgac
    42241 atcatcggct gatttgcaaa ttggtcgata cgaccagcag cggcttgagc agctacgaca
    42301 tctgtcccta gactatttag tgctgcttgt aacacgatag caccaacagc gatgatcgaa
    42361 gattgaaacg ccattggcaa agcagcattt aggtgtacag caaactcttc tttttcaaat
    42421 ttgaaatctt ttttgcgtaa ctgtaacaaa ggcatttttt tcttgatata cactagacac
    42481 agtacgctgg aagaaacttg tgcgattact gtagcaatag ctgcaccttc cacacccatg
    42541 ccaaaataga taataaagat caaatcaagt acgacgttga tgatgaccgc aatgatcaga
    42601 aagaatagtg gtgtgcggct gtcaccaagt gcgcgaatga cattagaaag caaattgaaa
    42661 ctgactgatg caaagatccc taataaaata attgaaataa atgtttgtgc ttgatcaagt
    42721 atttcaggag gagtttgcat caattgaagc atcggacgaa taaacaggag acttaggaca
    42781 gttaaaatca cagtgacgac aagactaatg acaacactag cagcaaaact tttcttcaga
    42841 ccccgataat cttttgcacc gtaacgttgt gcggtaataa tggctaaacc agctgttaaa
    42901 ccttgggcaa agccaatgat caaaaatgtc aggcttccag ttgcaccaac cgctgccagc
    42961 gcatttttac ctagcgtctg acccacgatg atcatatcga tcatattgta gaactgctga
    43021 aaaacatttc cgatcagtaa aggaacagtg aacatcaaaa tcagtttagc ggggcttcct
    43081 tttgttaaat cgcgcatctt ttaaactcct tatatcttta aaatttagtt agtagtatat
    43141 agcgtttatc ctgagaatac caattagaaa tcaatgataa tttatgaaac tccataaaaa
    43201 gaagccaaga attgagtcaa agaatcaaca acttggcctg gtctgtaaaa taagggacat
    43261 ccacgtttat ctatcacctt aatattgtct aatctcactt ggtaaaagga tcgtttctct
    43321 cgtaccgtca tcataggcgt atacttgagc aggaaattgg ttcgaatagg caaacggcaa
    43381 atcaatatcc atttccacat tgcttcctgc ttgagaaaaa tgcatcgtca cttttccacg
    43441 attttcgatc aaatcaaagg ttaatatatt ttctaatgga atgactccct taagatctag
    43501 gtcaataata aaccatatac tgtcaatcaa ttcatctggc aaacttgata cgacaccaac
    43561 tgaagcgtaa cggttgcggc tgctatcaaa actttcaaac atctgtttaa cctccttcga
    43621 atcaacgaat gttttatttt tataattaat cctaggataa actagaaata cttacttcaa
    43681 cgagggttca ttctaataaa gaatcaggaa aaatgcaaca ataattgaat actttttttc
    43741 aaagtataaa tttttataaa ggtacgataa aagtatgcga ggaaaagctt ggtaaattag
    43801 gatttttcgt ttgtaaagcc atttacaaaa gtggaagccc catatgaaaa taaacgatta
    43861 gaaataaaaa aggcgtgtta aaagctgctg acccgccgct tctaacacca cctgattgtt
    43921 tcagtattat tcttctgttg aaccgatcac ttctggttcg ccaccttcac ctgtgctttc
    43981 ttcttcttca ctttgagcga ctacagcagc gatttgctct tcactgtcgg tcacgaattt
    44041 gtaatcgtcg ttttttggaa gatcggctac tgtaatagaa tcacctaatt ccaagtttgt
    44101 cacgtcaact tcaattcgtt caggcaattt gtctggtgta gcagatacga ctactgtata
    44161 caagttttga gctaacactc caccagattt agcacccact gattcgccaa caagaacgac
    44221 ttctgtttcg acttctgttt cttccgacat gtttacagat agaaattctg catgttcgaa
    44281 atgattggta aaagtgttcg tttggatctt gtgaaccaat gtattgactt tttgtccatc
    44341 gatgtccatc gtgatgactg tgttcgctcc atgttcacgc aacaatttgc ttagtgtcaa
    44401 accatcgaat gagatagggg tactttctac tttataccca taaacaacag ctggaacttt
    44461 cccttcgtga cgaagtttgt ttcttaatga acggggacgt acttctcttt tacttacttc
    44521 taatgatact gacataacca aaattcctcc ttaaaatgtg actgtctgct ttggtaacgg
    44581 acagatttta tttaagtgaa ttctaaaggc taatctcgtg cacatcatcg aaggcagggc
    44641 gcttaaaaaa gcgcaaaagg agagatgcgc gcatctcccc ttagaaagtc aggattcaac
    44701 ttttctccac caggtcatta cgctgtgtat gagaaatcca attcacttta ctatttaacc
    44761 gtaacgtgaa ctgttaatta agtcaaggaa taactatgat gatgtatttg caagcaagaa
    44821 tattcaaggt ataattaatt agtagtaata agaaaggaaa cagcatgcgt ttagataaat
    44881 taatagaaga aaaattagaa acgacacgca aggaaatgaa gcggctgttt gctttgaagc
    44941 aagtgatgat agatggcaca gtcgaatttc gacaaaatcg aaatgtagat agtttactgc
    45001 atcaaatcga agtagcaggt gaacacttag aaacaaatga gtcctattat cttctcaata
    45061 aacctgaaaa agctgttaca gctgtatcag atccagaaaa gacgacagta atcgatctga
    45121 ttgcaagtgc cgatcgtttc caaccattat atccagtggg aagactagat agagatacaa
    45181 caggactatt gctgatcact tcgaatggac aattgggata tgaaatgcta cttccagaaa
    45241 aaaaggtttc aaagacttat caagtagttg tgaacgaaag ggtgacaccg gcagacgtaa
    45301 cagcatttga aaatggaatc gtattcgctg atgggtatca atgtaagccg gctgatttaa
    45361 aaatactcaa agcagacaag caccaaagta cggttttatt gacgatccaa gaaggaaaat
    45421 tccatcaagt caaaaagatg ttcttagccc aaggaaagaa agtgaccgca ttgaaacggc
    45481 taagcatggg accattggaa ttagatgaac agctaggagt aggcgagtat cgatcgttga
    45541 cattagcaga gatgaaaaag ttaagtattt attttaaata ggaggtcaaa aagatgaaat
    45601 ataaaacttt attgtttgat gtagatgata cattgttaga ttttcaatta actgaagcca
    45661 atgctttaag tgctttgttt gcagaacaag gaatcacatt gacaccagaa atcgaaacca
    45721 gctataaggc tttgaatcat catttatggc aggagtttga aaaaggcaga ctatcacgag
    45781 aagaaataat aggcagtcgt tttgggttac ttttccagca atttgggaaa acagtagata
    45841 gtgatcaaat ggaaaaacgc taccgccact acttgaacca aggtcatgat cttgttccag
    45901 gaagtctaga aatcctgaaa aaggtccatc cgcatgctga tctttatatt gtgacgaacg
    45961 gtgtgtcgca cacgcaacat cggcgattaa cagatgcagg aatgcttgat tacttcaagg
    46021 atatctttgt ctcagaagct gtcggagcgc aaaagccgat gaaagaattt tttgatcatg
    46081 tcttttccca cattcctaat tttgataaaa agcaaactgt gatcattggt gattctttga
    46141 cttctgatat caaaggagga aatatggcag ggatcgatac gatttggttc aatagaaata
    46201 aattaccaga aatcgaagaa atccagccta cttatcgaat cgattcatta gaggaacttt
    46261 attcattatt ggaaattgaa aaggattaat tttcagaaaa atatagcatt ttcgtgtttt
    46321 ttcgctataa tagattagaa tgaaaaaacg gatacgaaag aggttttagt caatgacaaa
    46381 aagcaagcca attatcattg gtgtgacggg tggatccgga agcggaaaga caagtgtcag
    46441 ccgtgcgatt ttcaacaact tcccggacca ttccatcatg atgcttgaac aagattctta
    46501 ttacaaggat cagagccatt tgagttttga agaacgattg aacacaaatt acgatcaccc
    46561 atttgccttt gataatgatc tgctgattca gcatgtaggc gacttactga actataaagc
    46621 cattgaaaaa cccgtgtatg actatgtagc gcatacaaga agtcaggcaa cgatcattca
    46681 ggaaccaaaa gaagtgatca ttctagaagg aatcctgatt ttagaagacg aacgtctgcg
    46741 tgacttgatg gatatcaaag tatacgtaga tacagatgat gacattcgta tcattcgtcg
    46801 tatcaaacgg gatatggaag aacggggacg cacattggac tccgtgatcg aacaatattt
    46861 gacagttgtt aaaccgatgt atcatcaatt tatcgaacca acgaagcgct atgcggatat
    46921 cattgttcca gaaggcggag aaaaccacgt tgctattgat ttgattacaa caaaagtagc
    46981 tagtttctta aaccataaat agtgcgcagc gaaacagttg aacggactgc gaccgtttga
    47041 aaagcaagga ttctgctttt tgaacagtca ccagccaact gtttttttat ttataacaag
    47101 aggttgtgaa aacagcatct tgacctaagt attaaaaaga agaaacggaa aatagctgtt
    47161 tatattttac cgttttttga gctaataccg aaggtcagct gattgagcac cgtgaagtag
    47221 aggttgtaaa aaagctgtct ttgcatcaag cgataagagc atccaaataa aacagctcct
    47281 cacgtttttc gttgggttgg gcttattatc gtagatgcaa gcttttgaac accgtttaac
    47341 gagaggttgt aacagaagtg gtcagatgca ataaataaga agaaacttac gaaaattatt
    47401 tctcgaattt gtgtgagatt tcgacttatt tttttgaaga agctcttttg tcgcaccccc
//
LOCUS       DACDIE010000016        42504 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.016, whole genome shotgun sequence.
ACCESSION   DACDIE010000016 DACDIE010000000
VERSION     DACDIE010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.016"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(148..2301)
                     /locus_tag="IV250_000991"
     CDS             complement(148..2301)
                     /locus_tag="IV250_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAP8768291.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
     gene            complement(2696..3544)
                     /locus_tag="IV250_000992"
     CDS             complement(2696..3544)
                     /locus_tag="IV250_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP8768292.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(3768..5708)
                     /gene="thrS"
                     /locus_tag="IV250_000993"
     CDS             complement(3768..5708)
                     /gene="thrS"
                     /locus_tag="IV250_000993"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAP8768293.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(6045..6224)
                     /locus_tag="IV250_000994"
     CDS             complement(6045..6224)
                     /locus_tag="IV250_000994"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HAP8768294.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(6271..8094)
                     /gene="cadA"
                     /locus_tag="IV250_000995"
     CDS             complement(6271..8094)
                     /gene="cadA"
                     /locus_tag="IV250_000995"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP8768295.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            8372..10651
                     /locus_tag="IV250_000996"
     CDS             8372..10651
                     /locus_tag="IV250_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8768296.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(10744..11391)
                     /locus_tag="IV250_000997"
     CDS             complement(10744..11391)
                     /locus_tag="IV250_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8768297.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(11570..12289)
                     /locus_tag="IV250_000998"
     CDS             complement(11570..12289)
                     /locus_tag="IV250_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP8768298.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(12420..13571)
                     /locus_tag="IV250_000999"
     CDS             complement(12420..13571)
                     /locus_tag="IV250_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAP8768299.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(13788..14528)
                     /locus_tag="IV250_001000"
     CDS             complement(13788..14528)
                     /locus_tag="IV250_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8768300.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(14532..14855)
                     /gene="rsfS"
                     /locus_tag="IV250_001001"
     CDS             complement(14532..14855)
                     /gene="rsfS"
                     /locus_tag="IV250_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAP8768301.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(14912..15505)
                     /locus_tag="IV250_001002"
     CDS             complement(14912..15505)
                     /locus_tag="IV250_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8768302.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(15489..16136)
                     /locus_tag="IV250_001003"
     CDS             complement(15489..16136)
                     /locus_tag="IV250_001003"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAP8768303.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(16149..16460)
                     /gene="yhbY"
                     /locus_tag="IV250_001004"
     CDS             complement(16149..16460)
                     /gene="yhbY"
                     /locus_tag="IV250_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAP8768304.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(16476..17585)
                     /gene="yqeH"
                     /locus_tag="IV250_001005"
     CDS             complement(16476..17585)
                     /gene="yqeH"
                     /locus_tag="IV250_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HAP8768305.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNEIQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(17582..18109)
                     /locus_tag="IV250_001006"
     CDS             complement(17582..18109)
                     /locus_tag="IV250_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HAP8768306.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(18289..19074)
                     /locus_tag="IV250_001007"
     CDS             complement(18289..19074)
                     /locus_tag="IV250_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAP8768307.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(19067..19936)
                     /locus_tag="IV250_001008"
     CDS             complement(19067..19936)
                     /locus_tag="IV250_001008"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAP8768308.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(19937..21316)
                     /locus_tag="IV250_001009"
     CDS             complement(19937..21316)
                     /locus_tag="IV250_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAP8768309.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(21324..21743)
                     /gene="fabZ"
                     /locus_tag="IV250_001010"
     CDS             complement(21324..21743)
                     /gene="fabZ"
                     /locus_tag="IV250_001010"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP8768310.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(21768..22244)
                     /gene="accB"
                     /locus_tag="IV250_001011"
     CDS             complement(21768..22244)
                     /gene="accB"
                     /locus_tag="IV250_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAP8768311.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVISEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(22247..23482)
                     /gene="fabF"
                     /locus_tag="IV250_001012"
     CDS             complement(22247..23482)
                     /gene="fabF"
                     /locus_tag="IV250_001012"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAP8768312.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(23501..24238)
                     /gene="fabG"
                     /locus_tag="IV250_001013"
     CDS             complement(23501..24238)
                     /gene="fabG"
                     /locus_tag="IV250_001013"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAP8768313.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(24250..25167)
                     /gene="fabD"
                     /locus_tag="IV250_001014"
     CDS             complement(24250..25167)
                     /gene="fabD"
                     /locus_tag="IV250_001014"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP8768314.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEATPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(25274..25498)
                     /locus_tag="IV250_001015"
     CDS             complement(25274..25498)
                     /locus_tag="IV250_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP8768315.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(25551..26513)
                     /locus_tag="IV250_001016"
     CDS             complement(25551..26513)
                     /locus_tag="IV250_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAP8768316.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(26523..26963)
                     /locus_tag="IV250_001017"
     CDS             complement(26523..26963)
                     /locus_tag="IV250_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8768317.1"
                     /translation="MESDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            27269..28234
                     /locus_tag="IV250_001018"
     CDS             27269..28234
                     /locus_tag="IV250_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP8768318.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(28291..28521)
                     /locus_tag="IV250_001019"
     CDS             complement(28291..28521)
                     /locus_tag="IV250_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HAP8768319.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(28607..29494)
                     /locus_tag="IV250_001020"
     CDS             complement(28607..29494)
                     /locus_tag="IV250_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAP8768320.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(29583..30806)
                     /locus_tag="IV250_001021"
     CDS             complement(29583..30806)
                     /locus_tag="IV250_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP8768321.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(30823..31902)
                     /locus_tag="IV250_001022"
     CDS             complement(30823..31902)
                     /locus_tag="IV250_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP8768322.1"
                     /translation="MLYYISEGGRKVKVLLYFESEKMLAKSGIGRALDHQKRALTEVG
                     ISYTLDEKEDYDILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQL
                     SPIVKKYLVGLYQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQ
                     KFREYFKLSPDQKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIR
                     KIVKEDHPENVLFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVR
                     DIPVYNGWLEDSKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEE
                     LKNVYESVLNEKVSSKLKKVNQVKD"
     gene            complement(32033..33760)
                     /gene="ptsP"
                     /locus_tag="IV250_001023"
     CDS             complement(32033..33760)
                     /gene="ptsP"
                     /locus_tag="IV250_001023"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAP8768323.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(33760..34026)
                     /locus_tag="IV250_001024"
     CDS             complement(33760..34026)
                     /locus_tag="IV250_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAP8768324.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            34505..36739
                     /locus_tag="IV250_001025"
     CDS             34505..36739
                     /locus_tag="IV250_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP8768325.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            36887..37207
                     /locus_tag="IV250_001026"
     CDS             36887..37207
                     /locus_tag="IV250_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP8768326.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(37412..37636)
                     /locus_tag="IV250_001027"
     CDS             complement(37412..37636)
                     /locus_tag="IV250_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HAP8768327.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(37834..39414)
                     /locus_tag="IV250_001028"
     CDS             complement(37834..39414)
                     /locus_tag="IV250_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAP8768328.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(39815..41191)
                     /locus_tag="IV250_001029"
     CDS             complement(39815..41191)
                     /locus_tag="IV250_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP8768329.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(41319..42437)
                     /locus_tag="IV250_001030"
     CDS             complement(41319..42437)
                     /locus_tag="IV250_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP8768330.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
BASE COUNT        12022 a         9148 c         7328 g        14006 t
ORIGIN      
        1 ttttgtcaca accttttttg ccgagtaaac ggtgttcaaa agccagtttc tcggtatgaa
       61 gtcaaattta agcaaaaatg tgagaagcta ttttcacaaa atttttcttc atactcgaaa
      121 ctaagcggct ttttcacgac ctctttttta ttttgccatg aacatatcat aatacgcgtc
      181 cattatttct ttagcaatcg tcaagtttat gtggtcttgt tcgtctaata agttcggcaa
      241 gacaacactg atggcaattt ccggattgtc tgttggtccg tatgccacga tattactatt
      301 gactgtcgca atatctggat gaccttccgc aaccgtttct gcagtccctg tttttgcgga
      361 aaggtccatt ttcgcactgg ctaatggttt ggctgttgtg taaggatcgg taccatggac
      421 aacttggtgg aatccttcac ggaggatcgc catattttct gcagaaatat tgaccgtatt
      481 ttctactgtt gtgccaattt ctttcttcac ttcacccaag tttccatttt cgtcattgcc
      541 atagatcgct ttgactaggt gcggctgtac tcgtttcccg ccattagcga ctgttgaagc
      601 atattgagct aattggattg gtgtatatgt gtcaaactgt ccaaatgcca aatcggtaaa
      661 gttacctgga gcataccatt ctttgccatt atccgcattg aacttcttca tataatcaac
      721 agaacgtgag atcccaggag attcattcgg caaatcgatc ccagtcgttg taccaagacc
      781 aaactctttg aaagagtctc tcaattgctg ataagcttta gcttcttcat ctaaactagg
      841 tagtcccatt ccaggtgtat agtctagtcc taacatcttt aacgccactt tgatcatata
      901 cgtattagaa gacaattccg atgctttgac tgcatcaaga gcaacttgtc cgcttcgatt
      961 aaagacagag cttttttctg aagcaccttg cagacgaatc ggctcatcaa tcaaaacttg
     1021 atttccgctg atcgcgttac ttgcccagcc tgctgtcaaa gtaccagcct tgactacaga
     1081 ccctggatca aaggcgctag taatcgtgcc taaagcattc tcggtgattt ctttggaacc
     1141 ttgttcatga ctgaaaccag tcattgctag cacttctcct gtctgaggat tcatagccac
     1201 tgcataagca ccaggcgaat actgtgcttt gccgttgttg atcaatgttt gataattcct
     1261 tttcaaaatt tcttctactt ttgattggaa ttctgcattc atagataaca ttaaattaga
     1321 acctttttcc ccagagaaga tttctttttg attcgaaaca tttccttcat tatcaaggga
     1381 aacttcatat tgggttttgg ttccttgcag gacatcttca tattgttttt ccaaataact
     1441 ttgtcctaca cggtcattcc gagaataacc ttttgctagg tatttctctg cttcatctgc
     1501 tggaagtcct tgcttttcag tcgtcacact gccaagaata cttttcagtg aacttgctgc
     1561 attatattct cgtgtccagt ctgtgccagt cgaaactcct ggaagttcag aagcgcgttc
     1621 tgccacaaca gctaactctt gatcagtaac accagagttt ttgatataga cagtgtttaa
     1681 ttcatatgca ctattcatcc gtttaaagat ggtagcaatt tttaattgct cctcgtttaa
     1741 attaatctca tcagctgtga ctttattgac catcgcacta tattgttcgc tgttccctaa
     1801 ttgcttttct tttgccgaaa gtctttttgt ggcttctttc aaatgtttgg gatcagccag
     1861 ccaaaaatct tttttatctc tatctgtcag attttcatct accggaacat cgatcagcga
     1921 gttcagcttc gttgcgattt caagcaaatc tttagccgta acttgtgttc ctctcgtata
     1981 tgtgatggcc tgattcgctt tattcgtaac taaagctttt cctgtggagt cgtagatcat
     2041 tccgcgagga gtactttctt ggaccgtaat cgtagaagta gacttcactc gcgcctccat
     2101 ctcttcgccg ttcacgatct gcaaataacc caatctagca atcaacgcaa caaataacgt
     2161 aaatataatg aaaaaaagaa aatttaagcg aaaaggaaca tgggaccggc gaaacgtttt
     2221 gttcttaggt tcttgtgcac cttttcttat tttttctaac caagaagtat ttaatatttt
     2281 tttcataaaa tctttcatca tttcctatcc ttccgcctat ttacattcat gcttcattgt
     2341 acccaaaaac ttcttaataa acaagaagta tttaatattt ttttcataaa atctttcatc
     2401 atttcctatc cttccgccta tttacattca tgcttcattg tacccaaaaa cttcttaata
     2461 aaaaagaaaa aatgggcgga gcaagaaatt ctttacctaa tttcaagata aaatcaactt
     2521 tttgtttcac agcaatcact taaagttaca tatccaacta tttttttcat taaaaaataa
     2581 acttattttt gaaaattatg ttttcttaag tcgtgaaaca ctttgatgga tcatttattt
     2641 gtggaaaact acgttttgtt attctaaaaa acttatataa aaaatagtaa ggtttttatt
     2701 tcgaatccat aaaatattct tggattgctt ctcgtgtttt tttcactttt tcgctaagct
     2761 tttcgttttt agccatatag aaataaaaaa tgatatcgat cgtagcaaat tgggccaaaa
     2821 gtgaattagt tgccgcactt cgattcgtga tttcttttgg tctagatgtt tgtacgagta
     2881 catcggctat cttgctaagc gggttatttc caaattgcgt caacgcaatg atttcgatat
     2941 tcatgctttt cgccagctcg gctaatgcga ctacatctgc acttctacct gagtttgaaa
     3001 caagccaaag cactttcttt ttctcattgc tgacaagttg cgggagcaag acgtggatat
     3061 ctttttcaaa aatgattggc ttaccgacac gagaccattt ttgcagaata tcttctacta
     3121 ctaaagaaga ggcgccaacc ccatataaaa aaacagtctc tgccgattcc aaacactcta
     3181 cgactttttg gatcgtttct tcttctagaa tatctactgt ttcttttatc gctacttgtg
     3241 cattgttacc aagtttgttt ttgatagagt aaaaggactc atttgcttct atatcactaa
     3301 aaccttcatc gattccagtc tgtagttctg ttgaaatcgt gattttgaag tccgtcagac
     3361 tttgaaaacc gatttttttg gaaaaacgta caactgtcgg cgcactcaac ccggaagctt
     3421 ctgctaattc attagctgtc attgtaggga ccttctcaat attaccgata atgtagttgg
     3481 ccaacttttt ttctgctgaa gaataatctt ccatcatact ttcaatacga ctaattacgc
     3541 tcataagttc ctccaccctt ttaatgatag tctatgtaag tataccatat cagaccctta
     3601 ttcacggtcg tatactggat aaaaaacaaa aaaacaatcc gcccaacaga atatcttatc
     3661 ccatcaatcc cacagattct attgagcatc tgtgtctttg aggaatacct gttatctatt
     3721 gttcggattg ctttctatct actacttttt tacaggaaat ttacctatta gttttctctg
     3781 ctgtaatttt tgacttctgc ttccatagca tcaataaaga tattcaaatc ttctacagct
     3841 gtttctttac ttccataacg acgtacgttc actgttgcat cttccaattc cttgtctcca
     3901 acaactaatt ggtaagggat cttttgtgtt tgagaggcac gtatcttata gcccattttt
     3961 tcattacgat catcaacttc tacacgcaac cccttcattt gcaaacgttc tttgatttca
     4021 taagcataat cgccatgtgc atcaacagaa acagggatga ttgttgcttg tataggtgcc
     4081 aaccatgttg gaaaagcgcc tttgtatact tctgttaaat aagcgacaaa acgttccatt
     4141 gtagaaacaa ttccgcggtg gataactact ggacgatgcg tattttcccc gtcttctcca
     4201 acatatgtca aatcaaagcg ctctggcaat aagaagtcta attgtatagt tgataatgtt
     4261 tcttctgtac caagagctgt tttgacttga acatctagtt ttggtccgta aaaggctgcc
     4321 tcgccttctg cttcaaagta atctagttct aattcatcca tagcggcttt cagcattgct
     4381 tgggcttttt cccacattgc gtcatcatca aaatatttat ctgtattgtt tggatcgcga
     4441 tagctcaagc ggaaacgata atctgtaata ttgaagtcag catatactgc caccatcaat
     4501 tcaagcgtac gtttaaattc atctttgatt tgatcaggac gaacaaatgt atgaccatca
     4561 ttcaacgtca tttctcgtac acgttgtaaa cctgacaaag cacctgattt ttcatagcga
     4621 tgcatcattc ctaattcagc gatacgaatt ggtaattcac gataagaatg gatcgtgttt
     4681 ttgtagacca tcatgtgatg agggcagttc attgggcgaa gaacaagcat ttcgccatct
     4741 cccatatcca ttggcgggaa catgtcttct tgataatgat cccagtgtcc agatgttttg
     4801 tacaactcaa catctcccat tattggtgta tatacatgtt ggtaacctag actaacttct
     4861 ttgtctacga tataacgttc gatcgtgcgt cggattgttg ccccttttgg aagccagaat
     4921 ggcaaaccag aacctacttc tggggaaacc atgaacaagt caagttcttt tcctagttta
     4981 cgatggtctc tttcttttgc ttcttcacgg agacggatat attctttcaa agctttcttg
     5041 tcaaaaaagg ctgtcccgta tacacgttgc atcatttgat tgtttgagtt tccacgccaa
     5101 taagcacctg ccactgataa taatttaaat acttgtatac ggccagtgga tggaacatgt
     5161 ggtccacgac acaaatcgat gaattcgcct tgttgataag cagtaatcac ttcttcttct
     5221 ggcaattcac taatcagttc tactttgtac ggatcacttg caaaaatttc taatgcttcc
     5281 tgttttgaaa gaacacggcg ttcgatcggc agattttctt tgacgatttt catcatttct
     5341 gcttcgattg ccggcaaatc ttctgcagtt acttggtttt gtccattatc tgtatcgtag
     5401 taaaaccctg aatcgatagc tggtccaacg ccaaagtgga tatttggata gaggcgacgc
     5461 atggcttggg ccatcaaatg agctgctgaa tgacggacta agccaagtgc atcttcatgg
     5521 tctggtgtga tgatctcaat acttgcatct tcctcgattg gacgagtcaa atcaacgagt
     5581 tttccgttca ctttaccagc taaagctttt tttgctaagc tttttgaaat gctttcagca
     5641 attgccaatg ttgttgttcc agactcaaat tctttgactg caccatctgg aaaagtaatt
     5701 tttatcataa tagtttcctc cttgaaatgt tttgattgat cagatcatca agctgaaaaa
     5761 caaaaaaatc ctagcatctc ttgaaaagag atactaggac gaaaagttcg tggttccacc
     5821 taatgttcaa acgcaaatgc gttcctcaag cgttgtaacg gtccgaccgc ccttgcttca
     5881 acaagggttt cgaaagtggt catcctttgg ttttctcctg gaaaagtttc agcctagctt
     5941 ttcctctctt atcgaagact ctcaaattca gttggctttc tcatcaatca attatgtgtt
     6001 tatttaacca ctaataaata gaaaaatcaa gtgggaaact aaacttattt tttccattcc
     6061 ccatttacac cgtatgttcc ttttttcatc tcattgatga tgacatggat gtgttctttt
     6121 ggtgcctgcg tgtttttatg aacagcttcc gtgatatctt tcatcatatt ttctaattgt
     6181 tccggactgc gaccttctac taattcgaca tgtacgaatg gcataatgct ctctcctctt
     6241 tctgatcgtt cttttttcaa ctcgattcta tcatactatg tttgtcttgg gtactgaatc
     6301 aggaaaaatg gctggatctt tttttgctct caaggcactc aaaatagaga ccgtatccac
     6361 gacttcttgc aaaacagctc cgaataatgc ggggattagt cccgtacttg caattaacat
     6421 caaaaaaata caaataaaga tgccaatcag tacagactgt ttcgctattc tcatcgtatc
     6481 tcttgaaatc actactgctt ttgctacacg caagagatca tctttcaaga tgaccgcatc
     6541 tgcactttca ctagcagctg tagaaccatg agcccccatt gcaataccga tatctgcagc
     6601 agcaagtgct ggtgcgtcat tcacgccgtc tcctaccatc gcaacaggtc tttgatcttt
     6661 agggatactt tctaaaatgg tcagtttatc ttgaggcaag ctacgggcat gtacagaatc
     6721 taattggact ttttctgcaa tcgtttgagc aatggcagat ccatccccgg tgatcatcat
     6781 caaatttttc acatcaagtt ttcttagctc cttgattgtc tttgctgctt ctgggcgaat
     6841 ctgatccgaa aataggatcg ttcctgcaaa agatcgatca attgttacat agattgctgt
     6901 attggacgtt tctatttctt cagctgaaac aaaagaggca cgtccaatct ttacttcatg
     6961 attattgact actgcttgga ttccttgacc ggtcacttct ttcagttgac taacaggata
     7021 cagttggctg tttttcacgt tgttcactaa cgaacgagct aagatatggc tagattcctg
     7081 ttctgcacta gctgcataca gaagtagctc ttcttttttt attccattga ctggctgaat
     7141 cgcctctact tccaatatcc ctttggtaag cgttcctgtt ttatcaaaag caatcgtttt
     7201 tgtacgtgcg agcttttcaa tcgttgttcc agtttttaca acgatcccgt ttttacttga
     7261 acggctcatt cctgcaacta aagcaatggg cgcagctaga atcagtggac aaggtgatgc
     7321 cactaccagt acctcagcaa atcgtaaagg gtcttttgag aaccaccaag aaataccacc
     7381 gatcaaataa gcaatcaacg tgaaagggac tgcataacga tcagctaaac ggacaaatcg
     7441 ggcaggcttt tcttttgatt cttcgactag tttaataatc atttgatact ggctgtctgc
     7501 ggcacgtttt tcagcgatca tttggatcga tccgtctcca tttaaagacc ctgacataat
     7561 ctgatctcca ggtttcttgg tttccgggcg ggactcacct gtcaaagaag attcatcgac
     7621 cgaagattct cctttggtca ctcgaccatc tgcgggtaca agttcgccag gcttgactag
     7681 taactggtca tcaatattta tttcatcgat tactatatcc tgcaattttc cttctttgaa
     7741 tcgatgggca attcttgggg tgttatctaa caaagactgc agttctttac ccgctcgttt
     7801 attcgcgtaa tcctctagac tatctccgcc cgtaagcatg atcaatatca tcaaactagc
     7861 ccagtattct ttgacggcta atgttgcaag gattgcggta atcgctaata catctacgcc
     7921 atatcttcct gaacgtaacg tcttgatcat ttccaaaaac atcaagaagg cgagaatccc
     7981 tccagtaaat gcgacaatcg caaatgcaag ggccggctgg tgaaaaacaa attcacttaa
     8041 taaagctatc ccaccaataa gcaaagtaag taaaaatttt ttgaaatgtg tcattgtttt
     8101 ttctcctctt ttatgtgact tagagaggtt agtaataaat ttccagctgc ctctttcatc
     8161 ttaagcatag tccttgacta gcacttttta aaacaatagg ccttaattga aaacaactat
     8221 cactatcagc gtttccaaaa ttcatcagtt ctacaaacaa aaatattcag caaggattac
     8281 tttttcgatt catcagttaa aaagtacctt ttttcgaaaa actatgctat acttttaatt
     8341 cgttatttac ggcggaagga gcaaacatgc gatgaatgag aaacaactag aacaaacgca
     8401 cgtggatgaa acgatcgaac taatccagat cgaacaaaat atgctggata caaaacaaca
     8461 agaattgact caggaaatga atcattcgac aaaagacaca gctaatcaca agattcgagc
     8521 tggttccaat gaagcttttt atgaatctgc tgttgaatat cgccaacatg agcaggaatt
     8581 gcttttgaaa taccatactc ttgaagcaca gcaaaaacgc ctgcaaacgt tgtctgtcat
     8641 gaaagatagt ccttattttg cccgtatcga ctttaaagaa gaccgtgaaa cagaaacatt
     8701 atatctaggt atcgcttctt taagagatct ggcagaagaa acaatcgtta ttgactggcg
     8761 cgcaccaatt gccaatcttt attatgaagg tgagattggt cccgcatttt atgaaactga
     8821 aacagataga atagatgtcg atctgcagtt gaaacgacaa tttaaaatcc aagaaggacg
     8881 tattttatct atggtagata cgtcggaagt aatcaatgat gactttttgc tagagatatt
     8941 ggatgaatca tcaagcaacc atatgaaaaa catcgtctct acgatacaaa aagcacaaaa
     9001 tgcaatcatt cgggacacaa caagtaaagt aatgctgatt gaaggaatcg ccggaagtgg
     9061 aaagacatcc gctttgcttc aacgtattgc ttttctgttg tatcataatc gcaagtggct
     9121 cgaggctgaa aacgttcttc tattttcacc caaccatttg ttttctgatt acatctcgac
     9181 tgtattgcct tcattaggtg aaagcggcgt gcctacacag acattcaaaa attatttggt
     9241 gcagctgctg ccagagtttt ctttactaga tgaacagcaa caagaaagtg gttttttgct
     9301 aggggaaaaa gatccgatac aagtgatgaa aagcggattg accttagtcg atcagattga
     9361 tcgctatatc caaggcatca caaactacgg tcccttgttc cgtgatatga aaataaatgg
     9421 ccgtacgatc ctatcaaagg aatcgattcg ccaatggtat aaagaaacaa atgaacaatt
     9481 acctttacat catagacttt ctctattaca aacgaagctc ctgaaaaaat taggcggtct
     9541 gcaaaaagac gaaacacgtc agcaatgggt caaagatctg gcggaagaac agctccaaga
     9601 actatacgca acagatccaa atcttgaata tacagaacaa aaggagcgtt cattacgtaa
     9661 aaaactggcc aaccaaatcg tacgtaaaag attccgtcga atccatcgag ggatcatgaa
     9721 ttatcaattt gtcaatataa cgaaacaata tctccacttt ctacaaactg tgccactttc
     9781 tcttctaaca cgacataaaa tcagcgagac caggtggcag gaacatttga caactgttcg
     9841 tacacacttg cgtcaaagag aattgcgtca agaggatgct gtactattct ttttactgat
     9901 gcgccgaaca tatccagtga cggttacaca caaagctcgc tatatcttta ttgatgaaat
     9961 gcaggatttc gcaccatcac aagtggcatt gctgcaagcc ttgttcccaa aagcaaattt
    10021 gactttctgt ggagatctaa atcaaaatgt ctttggaaat gaaacgatcg ttggatcatt
    10081 ggaagacctt ttcgtggacc gtgaagtcac tcgtttccaa ttaacgacaa gctatcgctc
    10141 cacaaaagaa atcactgatt ttgctaatca ttttattgtg aaagaacatc aagtagaaac
    10201 aacagcaaga aaaggagacc ttcctttgct catccaagga acaacagatg atatcaaatt
    10261 gaattggtta gcagatacca ttcgaacgac tcagggaaaa gcccggtatt ggcgtacagc
    10321 aatcatagct aaaacacaag aagaatgcga agctctttat gctcaactgc cagacgagtt
    10381 gaaacaacaa gtccagctga ttttaaatga gagtgacttt atgaagcgtt ccattatttt
    10441 acttccagcc tttttagcaa aaggtctgga gtttgaccga gttttcttgt gggatgtaga
    10501 tgcagaacat ttttcttctc agcaagacaa attgatcctt tacaccatgt gtacacgtgc
    10561 gatgcatgaa ttgacccttt ttacctccaa taatgaaagt cctttgttaa acaatatgga
    10621 tatttcatct tatgagaaaa aagtactgta agtaaaaaga caactgctat cattaatgtt
    10681 agcagttgtc tttttattta caataataat tgcagaaaaa gtaattcaat catttaaggt
    10741 catttattct atatataaat ccaaatctgt ttcaggatat tttaatttga caagcgaaat
    10801 aatcattttc agttcttcca tgttttctgg atattctggg tcaaatacct tgctatgacg
    10861 gccaatcccg ccattgtcga tcaatcggta aattcgattc cattcaatga attggtcaat
    10921 tgattggaaa ttgctgattg gatagccgcc acaatccaaa atctctgata cacgaaatgc
    10981 taggtatgca ctatcgatca ctttattttt tttcgtgtct atcttctcat ttctttcgat
    11041 aagaattttt tcaggacgat gtttcaagct ccactctaat agtagaaaga aagcctccat
    11101 ccgatactct tcttcaaata accgaaaggc tgaatcagaa cgacgaaaaa gcagcggaat
    11161 cagataattt tgtctataaa tcagcattgg tgcgcggtcc accatacact ctaaataacg
    11221 gttcagatac cctgtaccga catagtttcc taatcgatag ccttcaacgc cgaatcttgc
    11281 gtatttttgc gctaattttg aacaatcagt tacgttcaca gctaactctt ctactttgcg
    11341 gtaaccgctt aagatacgtt caaaaggttc taaagtgatt tttgcttcca tattcttccc
    11401 cgtccatctt ccacatactt catatcattt cacatgatac ttatgctctc atcataccac
    11461 aggttgaatt agaaaagaaa ataaatcaca caaaaaagca agacaatctg ttagtgagat
    11521 tgtcttgctt ttcgaaaaag actgaatact tttcgttaaa actggttgct tagtcttcta
    11581 atgccacatt atggaacaca tgctgtacat cttctagatc ttccagtgca tcaatcattt
    11641 tttcaaatgt ttcttgatct tctggagata gtgttacctc gttttgaggg atcatttgga
    11701 tctcagcgat tgaaaaatca gtgattcctt tttcttttaa tgcttcttgt atactatgga
    11761 aatccgctac ttcaccgtaa acgatgattt gtccttcttc ttccattaca tcgcgaacat
    11821 cgatgtcttt ttccattagg tattcaagga tctcgtcagc gtcttctcct gcaaaagcaa
    11881 agattgctgt attgtcaaac atgtaagaaa ccgctccgga aacacccatg ttcccgccat
    11941 ttttcccaaa tgctgcccgt acgtctgctg ctgttcgatt cacattgttc gtcaatgtat
    12001 caacaatcac cattgagccg cttggtccaa atccttcata gcgcaattca gagtatgttt
    12061 catctcctgc accttttgct ttttcgattg ctcggtcaat aatatgtttt ggtacattat
    12121 aagttttagc gcgatcaatg acgaaacgta gtttttggtt tgcatgcgga tcaggatcac
    12181 ctgattttgc tgccgcgtag atttcaatcc caaattttgc ataaatccgg ctattattag
    12241 catctttagc agttttcttt gctacgatat ttgcccattt acgtcccata ttttcacttc
    12301 gctttctgat ttgatagttt cataacgcaa aaacgtcctg ttccattata cttgtgaatc
    12361 acgattctgt gaagcttttt ttctttcttc cttcattctt ctcattttta gtcaattatc
    12421 tatactcgga ttgggatttg cccgaaattc tgttccggaa tattgcctcc caattgatag
    12481 atttgatcac ttctaactaa cagttccgct tcttgtgcct ctttttttcc gattcgggaa
    12541 taaatagaaa tctctttttt ttcttttaag aatcttcttc cagcatcggt aaatccaagt
    12601 agtcgaagat aatttttatt ttgttggaca ggaatctctt gctttttgat attcagcaag
    12661 acataacaag ataatcgttg gatccgtgtc catgtatatc tttttgtttt tacctttgac
    12721 acaaaatcct caaaactatc agcagttgca gctgctgttt tcatgcgcga ttctattcct
    12781 tcggtcatct gatagatatc tgtcagttcc agcaaagtag aagataacaa ccgataacgc
    12841 aaatacggcc aataagattc ccaagtctgc agcgaacaag ttgaaagatc ttcttctgtc
    12901 tgaggcggca caagtccagc tattcttttt ccttcttcta acgcatggcg aatggctgtt
    12961 gcactggcta tcctctgatc tgtgatgatt ttttcatgat agcctgcact tttacgtcgg
    13021 atcggatgta attccatagg gtggacatac ttggcatttt cctttgcata acttaatccc
    13081 aagatatgat ttggcgaaga aaaatcaagc gtaatatcgg gatacagact gcgaaaaacc
    13141 tcagtcattt tttgcggata actatacgtt tgatcagaca gttcttgaaa tgcctcttct
    13201 atcctatctt gattctcttg gacaaagcgt ccgaaactgc tgtaatcaaa cgaagtctct
    13261 tcttctgttc caaaacacaa actgtcacag tccagtgttt gcaagatttt tactgcacct
    13321 ttagcaaaat agtcagctga ctgaaccgcc cattcgacgg gaagctccac tactaaatcc
    13381 acaccatttt gtaaagcttc attcgcgcga tgccatttat cgatgatagc cggttcgcct
    13441 ctttgcaaaa aattcccgct cattacagca atgacgacct ctgctcctgt tatttttcgt
    13501 gccatttcca catgatagcg atgtccattg tgaaaaggat tgtactctac gataatcccg
    13561 cagcttttca tgcgcatcct ccttcatagt tatcgataaa aaagaaatag agacaagtaa
    13621 aaaaaggcaa aaatcggaaa aaatacccga actatataaa ctattctcgc aaacacaata
    13681 cttctgcgac gaaaagctgc actgcaagag caatgcctat atcgttcgga tatataattt
    13741 atgttgaaga tttattcttt tcttgttaac tcgagcaggc aaaaccgcta gtctttgtat
    13801 gctacaaaga accagcgctt tgatttctct gatgctgctt cgtctgtaaa atcagaaaat
    13861 gcttcgactt tcgaaaagcc gctgttttcc agcattctta aataactttc caatggatag
    13921 gtacgctctt gatgtagttc atcttcacga ataaatcgac cagacccttc ttgttcttca
    13981 acgaaaaagg taagaaaatg ttcgatgctg tgtttctcgc ttcctggata actatcccac
    14041 aaaaaagcaa aattatcccc ttgataatga tagctatatt ctggaaaaac ctcatctact
    14101 tgataggtgg agtgtacgtc aaataaaaat cggccatttt cttctaacgc ttgatagact
    14161 tcatcaaaca cttgctgtac ctcttgtcta tttttcatat agcacagcga atcagaaaaa
    14221 caggtaattg cctgatattg tccgatttct gataaatcga gcatattgcc ttccacaaat
    14281 tggatcgatg cgtcttcttc atatgctcgc tggctggcaa tcatcaacat ctcttctgaa
    14341 aggtctaacc ctgtcacttt atatccatct tttgcaaagg caaccgctaa tgctccagta
    14401 ccgcatgcca gttccaaaat attttttgtg tcttcttgta aatgacgttt cgaaaaagct
    14461 aaccattgct gataaagtgt ttcgtccatc acttcatcgt aaatgaaagc aaatgtttca
    14521 taagccatat gttaatccaa ccattcacta agatcgacta gcggagcatc tgaccatagt
    14581 ttttccagat tatagaaatt tcgttctggc gcatggaaaa catgtacgat cacatctcca
    14641 agatcgatca atacccattt tccgccttct tttccttcaa tacgttttac ttcatatttg
    14701 ttttcttctt ctttatcaat gatttcttct gtaattgcgt tgatttgacg ttcgctattt
    14761 gctgaacaga tcatgaaata atctgccaac aaggaaacat ttcgaacgtc taaagcgata
    14821 atgtcttctg cccgttttga atctgctgct tcaatcgcaa tttttaattg ttctttacta
    14881 tctatgatga tttcctccta atatgagcag cttatttttt tgctacccag taattatatg
    14941 tttcgattgt ttttggataa atctgttgtt cttgcgcaag caaatgggcc aatgtatgtt
    15001 ttgtttcaaa tgctacagct tcgtctagat cagcccatgc aatggctcgt gcatcttgta
    15061 ccccaggaaa atctcgacct ggttcaatat agtctgctac ataaatgatt ttgtcaagga
    15121 gactcatctt agcagcccca gtcgtatgca cccggattgc atgaagaatc tctgcatcgg
    15181 tgattcctaa ttcccgctct acaaacgaag cgccaacaag accatgccag attgcattat
    15241 tataattcaa taactccgga tcaaaaccgt ctcttacgat taccattttg aactcctcat
    15301 ctggtcgttc ttttgcataa tcatgtgtca atgcagcaat actagctttt tcaggagaag
    15361 caccgtattt tttggcaaga gccacggctg tttcttctac tccaagcaca tgcttgaagc
    15421 gttgttcgct cattctcatc tgtatttttt gcatcaggtt ttctctagaa taagcagtat
    15481 attcattact atagtccata ttcatacaac ccttcatttt gaatataatc aattacttta
    15541 tctggaacaa ggtagcggat agaacagcct tctttgatct tttgacgtat tttcgtggaa
    15601 ctgatgtcga tctcaggtac atctacccaa attacaggat atggagtatc cgttgtgtat
    15661 cctggtcgac gtatacccac aaagttgacc atagaagtca gctcatcgat tttataccat
    15721 tttggcaaat attctaccat atccccaccg ataataaagt aataatctgt atccggattg
    15781 ttttgcgtca attccttcat agtatcatag gtgtaacttt tcccaccacg agctaattca
    15841 atcgtttcaa tctgcaaaaa aggattgtct tccaccgcta attccaacat gttgagtcga
    15901 tgttttgcat caatcgtctg tttctcatca acatgaggtg gctgataaga aggcatcaaa
    15961 aatacgcggt caagacctaa attccggcca gcttgttcag ccatcactaa atgtgcaagg
    16021 tgaacgggat tgaatgttcc gcccaagatc cctacttgct ttttcttctc taagaattga
    16081 ggcatttctt caggaaataa ttgtgaacga acaaatgtct tagcttgcgt attcatcatg
    16141 gtcactcctt atattgcttt tacttctttt gatagccttt gatacttttc ttttgtggaa
    16201 ggtttgtata atacaataac acgtccaatg atttggacga tttcgcaatc tatttctttt
    16261 tctaatactt gagctgcttc ttctgcaatc tcatctgtat tttgaagtaa cgatactttg
    16321 atcagttctc ttttttctaa agcttcttca atttgaacga tcattgctga attgatccca
    16381 ccttttccga tttgaaaaat cggctgaaga tgatgcgctt tgctacgtaa aaaacgcttt
    16441 tgttttcctc ttaaactcat aattttcctt cttccttaaa tcaatgcttt gcgtttcaat
    16501 acatctacac cctttggcgc ccagcccgca attactactg gcgctgttac agtgatccag
    16561 cctagaccgg caaaaacgat gtctgttttt tctttcacag aaaactcaaa acgaacaagc
    16621 tccggaaatg cctcgacttc atcaggacga ggaggctgta ataatccacc tacatgtttt
    16681 tgataaaatt cgtctgcttt ttccagtttc gttcgatgaa tatttacatc atttgacaca
    16741 taagcaataa atgaagcacg attgccgctg ataaaatcaa atcttgcaag tccgcccaag
    16801 aaaagtgttt gcccttcatt cagctgataa actttaggtt tgatttcttt taacggtgca
    16861 atgatccgca aatctttttt acctaagtag tgcgccattt gatggcgatg gataattccc
    16921 ggtgtatcaa tcaaaaaatg cccgtcatct aaaggaatct cgattttatc caaggtcgtt
    16981 cctggaaact gcgaagttgt gattacatct tgtactcccg ctgtattttt gatgatttga
    17041 ttgataagcg tcgattttcc gacatttgtt actccgacga tatagacatc ccgtccatcg
    17101 cggtattctt cgattttttc taatagttcc gtcatctcat gagatttctt agcactagtt
    17161 aataatacat ctactggacg aattcctgct tcgtaagcac gttccttcat ccattgtgtt
    17221 aatttagttt ttttcaatga tttaggtaat atatccactt tgttcccaac taaaagtact
    17281 ggattgtcac cgacaaaacg gtgtagtcct gggattagtg aaccattgaa gtcaaaaata
    17341 tccaccacat tgacgatcaa tgcatctttt tgccctaatt cattcagtaa gcgcaaaaag
    17401 tcgtcatccg tcaatgagac atcttggatc tcattataat ggcgtaatct gaaacaacgc
    17461 tgacaataga cttccccgga agctaaacct ttttccaaag ccgtttgcgg cgtgtatcct
    17521 aattcaccgg gtttgtttgt ttgtataacg gcaccacagc cgatacattg taattcttct
    17581 gtcattcgat tccacctcgc cagcccatat cagggtgttt tgctaataga taggccataa
    17641 ttcttttttc ccaaaaacga ttaaattgtg tcttccaact atctgttgcg ataatgggtt
    17701 ttacaaggat gctacgtatc cctgcacgat tggctccacg tatatcagtc ataatctgat
    17761 cgcctaccat tactatctca tcatctgcta aatccaattg tttctttgct atgttgatcc
    17821 ctcttgctaa aggtttcatt gctcgtgcaa catacaaaag atcaaatttt tcaatcgctc
    17881 gcgcgacacg ttttgaatta ttgtttgaaa caacaacaac agggattcct gcatttttca
    17941 tttctaaaat ccaagtaagc aactcttctg ttccatctgg attattccat gcaatcaacg
    18001 tattgtctaa atcggtcaat actgctttga tgcctagatt ctttaattgg gcaggtgtaa
    18061 tctgatagat ggcttctacc atccatgttg gtttgtaatt tgaaaacatg ctgtctcctc
    18121 atctgcaaaa aatgaaggag cacataaagt actcctcaac cccgtattat actgcatatc
    18181 agcaaaaaaa actacctcta tactcgttcg tctttcagaa aaagcatttc tttacaaaat
    18241 gaccaaaaat aaagattgat cacagaaaaa tttagaaaca aaaaacaact aatattggcg
    18301 gaacctcttg tatcgttttt ccagtaattt atgctgatct aatcgaacaa gttccgtcaa
    18361 tctttcaatc aacgcttttt tcataatgtg tgcgacttta tatcgtggca actgctcgcc
    18421 gttgaacgtt tcaggaataa ctcgatcaac gattcctaac tcgcgtagtt ctggcgcagt
    18481 aattttcatc aactcagctg cttctgcggc gcgactaccg tctttccaca aaatcgacgc
    18541 aaatccttct ggagaaagca cagcataaat cgtttcttcc agcatccaga cttcatccgc
    18601 taaacctaga gcaagggcac cgccacttcc accttcacca atgatgatgg cgatgatcgg
    18661 tacctttaaa tcagacattt cgagcagatt tctggcaata gcctctcctt cgccgcgctc
    18721 ttctgctcct acaccgcaat atgcacctgc tgtattgatc aaggtaatca ccggtctatt
    18781 gaacttttcc gcttgtttca tcagtcgtaa agcttttcga tagccttcag gatggggaga
    18841 accaaaattt ctgcggatat tttcctgcag atcccgtcct ttttgaatac cgatcactgt
    18901 taccggtttg ccatctaatc gagcgattcc accaacaatc gcttcgtcat ctgcaaataa
    18961 acgatcacca tggcattcct cgaaagaatc gaagatctct tcaaaaaaat ccaagcttgt
    19021 ccatcgttct ttagctcttg ctaactgaac gacatcatgt gctgtcttat ccacgatcta
    19081 tccatccttt ctgtgtatgc aattcaagaa gcgtatggat tttctgtttc aattccatgc
    19141 ggggaacaat ctgatcgaca aaaccatgag ataataagaa ttcagccttt tggaaatctt
    19201 ctggaagttc ctgcttgatc gtctgttcga tgacgcgtct tccagcaaaa ccgatcaagc
    19261 tttggggttc tgctagaata atatcgcctt ccattgcaaa actggcagtt actcctccgg
    19321 tcgtcgggtc cgttaaaaca gttagataaa aaagaccttc attgctatgc cgtttgacag
    19381 cagcagagat ttttgccatc tgcattagag aaaagattcc ttcttgcatc cgtgcgccac
    19441 ctgaagctgt gaacaagacg actggaagac tttcctttgt tgctcgttcg aataatctgg
    19501 tgatcttttc tccgacaatc gttcccatac ttcccatgat aaaattagga tccatcactc
    19561 caataatgaa gggaatcccc tgaatggttg ctttaccagt gaggactgct tcatgaagtc
    19621 ctgttttatc ttgcattttt ttaatttttt ccgtatattc gggaaatgct agcggatctt
    19681 tggttacaag atccgtatcc cattcttcaa aacttttttc atcaataatc aaagctagac
    19741 gctgccatgc accgattcga aaagcgtatc cgcaatgagg gcatactttt tcttcaccga
    19801 tgtctttcgt ataaaggata tgtttgcaat ttggacactt tgcccacatg ttatcaggaa
    19861 cagatggcga gttagcaggt ttgctctctc tattaggatt gatccgaata tatttctttt
    19921 tcttaaacag agccatctat gcgcctccta tctccgtttc tggtgtccaa ttcggtaaaa
    19981 attcttcttg taaaaatgct gtgctgtaat cacctgctat gactgcagga tgactaatca
    20041 aatccatctg aaattctgcg ttcgtgatga ttccttctgt gataaattca cttaaggctc
    20101 gctgcatctt cattaatgct tcaaaacgcg tatttccatg gacgattatt tttgcaatca
    20161 ttgaatcata atatggcggg atcgtatatc ccgggtaaac tgcactgtct actcgcagtc
    20221 ccatcccacc tgctggaagc agtaagtttt ggatcttccc tggagaagga gcaaaatgga
    20281 aagcaggatt ttctgcatta atccggcatt cgattgcgtg tccttggaat actacttctt
    20341 cttgacacac attcaaaggc tcaccggctg ctatctccac ctgttttttt actaaatcga
    20401 ttcctgtgac catttctgtt actggatgtt ccacttggat tcgagtattc atttccatga
    20461 aataaaaatc gccttcttca tccatcagaa actcgatcgt tccggcattt tcataattga
    20521 ctgctttagc tgctcgaaca gcagcgtctc ctagtgcttc tctttttgtt tgtgagatca
    20581 ctacagaagg agattcttcc agtacttttt gattgttgcg ttgtaaagaa caatctcgct
    20641 cgcctaaatg aatgacatga ccaaatcgat cacctaagat ctgaacttct atatggcgag
    20701 caggataaat gattttttcc aaatacatat catcatttcc aaatgccgct gctgcttctt
    20761 gctgagctga agaaaaatgc tgtgggagct cttcttttga ttgaactttc cgaatgcctt
    20821 tcccgccacc acctgcagct gctttcagca tcactggata accaattctg tctgctattt
    20881 ccaatgcttc ttctactgta gataatacac cagtacttcc tggaatgact gggacatttg
    20941 cttcttgcat caattgacgc gcattgatct tatttcccat tgcatcgatc gttgcacttt
    21001 taggaccaat aaatgtgata ttgcactctt cacacatgga ggcaaatcgg ctattttcag
    21061 ataaaaaacc aaatcctgga tgaattgctt cggcttttgt cacgattgca gcgctcagta
    21121 cttcctgaac attcagatat gaatcaacgg cttttgccgg gccaatgcag attgcttcat
    21181 ctgctaactg tgtatgaagt gcttcggcat ctgcttctga atatatcgct acggtttgga
    21241 tccctagttc gcggcatgca cgaatgatac gtacagcgat ttcaccgcgg ttagctatca
    21301 atactttaga aaacatattt cacctatcca atcataaagg ttaattccgc ttcggctact
    21361 tttttaccgt ctactttagc gattcctttg cctattccgg ctgaagcttt cactttcaat
    21421 atctcaactt caaggtataa ggtatctccc ggtgtaactt tcttacggaa ttttgctttg
    21481 tccaatccgc cgaaataagc cgtctttccc ttgaattgtt caagagaaag taatgcaact
    21541 gctcctgctt gagccattgc ttcaacaatc aatacccctg gcataacagg ttcttctgga
    21601 aaatgtcctt gaaaaaaggg ctcattgatc gttacatttt tcttagcaac gatccgttct
    21661 ccttcaacca tttcttccac tcgatcaatc aaaagcattg gataacgatg tggaatgatt
    21721 tcttttattt cttgtatgtt cattgttttc cctctttcct gctttgctta tttttgctga
    21781 atcctaaaga gtggctgtcc atattcgaca actgactcgt tttctacaag gatttctgtg
    21841 atcactccgt cggcctcact agttatctca ttcatcagtt tcatcgcttc aataatgcag
    21901 ataacttctc ctttggcaac ttgatcacct acttttttga aagctggttt gtcgggcgct
    21961 ggttgcagat aaacgattcc cacgattggc gaaacaacct cgattccatc aaatactgcc
    22021 gattcttttt ctttcattat ttctggagca gttgttgctt gttcagatat gacattgact
    22081 ggctccgaaa ctggcaacgt ttcgtttgtt tgaaccatat tcatgcctcg acttgtttta
    22141 tttttattca tatacaattc aaattgacct tcacgcaaat cgaattccgt tagcgaggat
    22201 tgatcaaatt gactgactaa ttctctgatt tcattgatat ccaaaattat tcctcccagc
    22261 gtttcatgca cagaaccgca ttatgtccgc caaaccctaa tgaattactc attgcataag
    22321 taaaacttgc aggtttgctt tcgtttttga caactgtcaa gtcaatctca ggatcttgtt
    22381 tttctacatt gatcgtaggt gggataaatt gatgttctaa tgccagcaaa gttgccaagg
    22441 cttcgatccc gccggctgct cccaataaat gtccagtcat cgatttcgtg ctgctgacgt
    22501 aagtatctct gaaattttca cctaatgcga tttggattgc ttttgcttct gcctggtcat
    22561 tggctggtgt acttgttccg tgagcattga tatatccgac ttgttccggc tgtacaccag
    22621 cttcttccat cgcaagcttc attgctttag atgcaccaga accatctgga gtaggagctg
    22681 tcatatggta agcgtcacag tttgcaccgt atccaacgat ttctccatag attttggcac
    22741 cgcgagcttg tgcatgttct aaagattcaa gaactagaac gcctgctcct tcacccatca
    22801 caaaaccatt ccgattttcg tcaaacggaa tcgaaccacg attaggatca gttgattctg
    22861 agagtgctgt taaagctgca aatccagcaa tccccatctc acaaatcgtt gcttctgttc
    22921 ctccagcaag aatgacatca gaatagccat gtttgatgtt acggaatgct tccccaattg
    22981 aattcgttcc gctagcacaa gctgttacga tagctgtaca aatacctttt gcacctacac
    23041 gcaaagcaat atttccagct gccatgttgg caatcgccat tggtacgaac attggagaaa
    23101 ctcgttttgc acctttttca tgtagacgaa taacttggtt ttcgattgtt ggaagaccac
    23161 cgatcccaga tccgatgatg acgccaaacc ggtcttgatt cattttttct ttatctaaac
    23221 cgctcatctc cattgcttct aaagcagcgt agattccata gatcgaaaac atatccattc
    23281 gtttgctatc ttttttaata aaatatttat cgtaagggaa atctttgact tcacccgcta
    23341 aggtaatccc tgtttctgaa gcatcaaatt tagtgatcgg tgcgatgcca ttcgtcccct
    23401 cttttaagct attcaaaaag ctgtctgcgt catttccaat aggtgaagtg actccatagc
    23461 cagtaattac cactcgattc atttgtttgc tctccttttc ttatccgtgc atgaccaatc
    23521 cgccatcgac attgatgact tgtccggtga tatatggatt ttgtgctaag aaaactgctg
    23581 tttgtgcaat atcttctact tgtccgaaac gctgcatcgg aatttgcttt tccgcttgtt
    23641 ccttgacttt gtctgctaaa acttccgtca tatcagttgt gatgaatcct ggcgcaatgg
    23701 cattacatgt aatgcctcta gttgcagctt ctcgtgccac agattttgtt aatcctacta
    23761 cgccagcttt actagctgca taatttgctt gaccaatatt tccaatcaac ccagatacac
    23821 tagagagatt aatgatggcc ccttctcgct gcttcatcat ttttttcaat acgtgctgcg
    23881 tcatattgaa ggttccaatc aaattgatat ccagacattt tttgaaatct tctgcatcca
    23941 ttcgcatcac aagtttgtca tttgtgattc cggcattgtt tactaacaca tggatcgatc
    24001 caagcttttc ttctgcttct ttgatcattt gtcccgcttt ctcataatca gagatgtcac
    24061 cggaaactcc tacgcatttt acaccgaatg cttcgatttc ttctatcttt tcttttggaa
    24121 tctctccgcg accattcaaa atgatatttg ctccagcttt tgcaaaagct aaggccatcg
    24181 ctttgccgat tcctcttgta ctacctgtga taaatacatt ttttcctttt acatccataa
    24241 cgttcctcct tatagtattg ctattgtttc atcaaatgtc tgcttattct caacacgcag
    24301 aactggtatt gttttatcga tttttttcat aaatccgctt aatacttttc cggggccgac
    24361 ttcgatgact tgttcgatgc cgattgtttt taacttatgg atactctcat agaaacgaac
    24421 aggttgcatg acttgctgtt caagtagtcc cgcgattgtt tctttcttca ttatttccgt
    24481 tgttgtgttg cttatgatag gaaaagaagg ttcagagaat tgaatctgac ttaggtcctt
    24541 cgccagtttt tttgctgccg gttctaaaat agctgtatga aaaggcccgc tgacatttag
    24601 agggatcatt cttttgaacc ctttttcttt taatagtaaa acagcttcgt ctactgcttt
    24661 ttcttcgcca ccaatcacga tctgctgagg tgtattataa tttgcaggag atacgatgcc
    24721 gtacttgcta gcttcatgac agctttcctc gatcgtttca atcggcgcat tcatcactgc
    24781 aaccattttc ccgcttccag ctggggtagc ttctgtcata taagcgcctc tttttgctac
    24841 taacgcaact gcttcagtga aggatagcgc attactagct actaatgccg aatattctcc
    24901 taaactcagt cctaaagctg catctgggat gattccatgc tccttcaata agcgataata
    24961 cgcgatgctg actgttagaa tagctggctg tgtatattgc gtctgatcca aacgttcatt
    25021 ttctgtaaag caaagttcag ccatatcgta gccgagaatc tcacttgctt catcaaatgt
    25081 ctgctttaca acggcttctt catataaatc ttttcccata ccttgatatt gtgcaccttg
    25141 gccactaaat aagaatgctg ttttcatgtt tttctccaat atatccagaa tatatgtttg
    25201 attcttccta ttcaaaaagg ccgagacatt cctatttcag gaaatgtccc agcccccaac
    25261 ctaattttaa tgattatgca gtttgttttt ctacgtaagt cactaaatct tgaaccgttt
    25321 tgattccgtc ttctgtttcg attttcacgt caaattcatc ttcaatttca ttgatgattt
    25381 ggaataaatc caagctgtca gcttctaagt cttcttggat gtcagtagtc aatttgattt
    25441 cttcagtttc ttttcctaat tcttctgcga tgatttcttg aattttttcg aataccataa
    25501 taatatattc ctccgatatt gtttgctttt tatttttata atttaacagt ctacaactcc
    25561 atgagaatac ttccccatgt taaacctccg ccatagcctg taaatattac tttctgctgt
    25621 tttcctaagg tgatctttcc actagaaact gcttcatcca ataagatagg gatgcttgca
    25681 gcagatgtat tcccatattt atccatattg gtcaggaact tttcttgcgg catctttact
    25741 tttcgggcaa ttttctcaat aattctgagg tttgcctgat gcaaaagcag ataatcaact
    25801 ggtttgtctt tcatgacttc ttggatattg tttgcaacgt ctcgtacggc aaaatcaaaa
    25861 atatctctgc cagtcatttg caaataatac gaaccttctg aatcttctga atagaacgga
    25921 tttttatttt gctggtagcc agcggtcaat gagctacttc tggtaccgtc agctgctaat
    25981 ttttctttca gcaatttttt ctgtggattc gcttctaaga gaatcccacc tgctccatcg
    26041 ccaaacaaca cagcagtcgt acgatcagac caatcgatta gcttagatag tgtttcacca
    26101 ccaatcacca gtcccttagt tgagccgcag cggatcattt tttcagccat gcttaacgca
    26161 taaacaaatc ctgagcacgc agcactgata tcaaaagcga tagcatgagt tgctcccaat
    26221 ttcccttgta cttgtgctgc tacagatggt gtcgtgtaat cgggcgacat tgttgctact
    26281 aaaataaaat ccaaatcttt agggtttaga tttttctgtt ttatcaattt ttccgccact
    26341 aaaatgcata aatcagaagt ctcctgattt gttacacatc ttcgttcacg aatacccgtc
    26401 cttgacgaaa tccattcatc agaggtatcc atcatcttgg aaagatcatt attagtaata
    26461 atcttttcag gaaggacgct ggctgttgca gtaatcattc caaattgttc cattaaatat
    26521 cctcacttat agtcttgcaa gaagtcgtgc aagtttttca gtgcatccag caaggcatgc
    26581 tgttcctccg tagccattcc gtctaagatg ttcttgacca tttcacgatg aaaatgctga
    26641 tggacccgaa atagtaactt tccttttttc gttagtccca actttacaac gcggcggtca
    26701 tcttcactgc ggatacgttc gacatagcct ttttttacta acttgttaat agcagtggtc
    26761 aaggtcccaa cagtaattga tagttctttg gctacttctg aagttgtttt tttcttgtac
    26821 atcccgattg cctcgatcgt gtgcatctct gtgattgaca gatctttgaa ttgagatttt
    26881 ttcagctcag actcttcgat tgtcaaaata tcattgaaga cactaacaag ataatcattt
    26941 actgttttta aatctgattc catgttccac cgtccactaa ttagtttgat tatcaaatct
    27001 tttgatgttc aaactatatc ttaacttttt tcaaaatgca accttaaaag atcatttttt
    27061 tactatatga gcttttttta agattttact catttaaaac aactgataaa tcagcattta
    27121 taatttcttt tattagaaaa atcatctatc tcacatattt tgattatcaa agtattttct
    27181 gtgaacagaa ctatagaatt ccctaaaact tgtccattat ttcaaaattt gttatagtct
    27241 ttttattcaa agcgtaaaag gagatcctat gttaaaagaa aaaatgaaaa ctctccccat
    27301 gctgccgggt gtctatctta tgaaagaccg agatggtaag gtcatctatg ttggtaaagc
    27361 aaaacggctc aaaaatagag taagcagcta ttttcatcag aataaacagc attcaaaaaa
    27421 agtattacgt atgattcatc atataacaga ttttgatttc gtcgtagtag acacggaatt
    27481 agatgcttta ttgctcgagt gtcaattgat ccaacattac cgtccttttt ataaccgaca
    27541 aatgaattat ttcagtaatt ataattatgt acatattacc aataagggat ttgtattaac
    27601 agatactcct acggctcgaa catacgggcc ttttcgtctc tataaaaaaa tgccgtctat
    27661 tttgcgtata atggaagaaa cttatcaaat gccttggttg tctgaaatta gtttgttagc
    27721 tttgcgagtc cagcttcctg acctacagga aatgactttt gaacagaaaa aaaaggaact
    27781 ccaagggctg tttcaaggcc gaaataaaaa gctactgact tacttgaaaa aaagacaaca
    27841 acatttcatc tatcagttga atttcgaaaa agccggtatg cttcaaaaag atatcgaatt
    27901 agttacttat tttattagac ggatccagga acaaaagcag tttttaagaa caccatcgct
    27961 caccttcagc atgccattag cagcagatga gagtcagaaa aagcactacc tcgtttgtta
    28021 tggtcaactt gcagaaacaa tgattacctc tggtgacgtt tctcctgact tctattatga
    28081 aaagaaagaa gctcatttat ctttgaaacg tcaattgtca aaagaagaaa tcgatcctgt
    28141 acaaatcttg atcagttacc gcaaaaaatt agaaaaagaa cagtttgaaa ttgaactgtt
    28201 aaacaaaaaa gaagctgaaa agcaactaaa ttagttgctt ctcggcttct tttaggcatc
    28261 ttcaacttca aatgtcaatt atcctaaaat ttattggatt aagtcataga tttccatcgc
    28321 tacgatatcg atattatcaa attcgtattg ctgatgagta tctgtttcag ttaattcaaa
    28381 tgtatcggtc gctttatcat aagttactgt acacttctct acgccttctt tttcaaaacg
    28441 gcgaacttgc agttcattat ctgctgactc gaccatagcc tccagtcgtt ttatgattgc
    28501 gactaattgt gaatgtgtca tcggaggtcc tccttttctt atttctgtta tattgtatca
    28561 aaagcatagc ctacttgcat tgattttctc taaaaaaact cgttttttat gatttttttt
    28621 ctggtatttt tccttcaggc caccaaagtt tgatcaaagc ttgggttccg tcattctcaa
    28681 aacctttatc tgccaactct tcatatagtt tcaatgcttg ggtagttgct ggcaatacaa
    28741 gatccatttt ttctgcttct tctaatgcaa tctttaaatc tttgataaaa tgtttgacaa
    28801 aaaaaccagg tgtgtaatct tcttttaaaa tacgaggaga ataattgctt aaagaccagt
    28861 tagctgcact tccacctgaa agcgtctcaa tcactttctt taaatcaagt ccgttttttt
    28921 gtgcatagac aagcatttcc gtcattcctg tcatcgtacc tgcaatcatg atctgattag
    28981 ccatttttgt atgctgacct ttcccagcag aaccatgcaa tgtaaatgtc gtaccaaatt
    29041 ctttgaataa aggcagtact tgatcataaa cggcttcttc tccaccgacc atgatcgtca
    29101 gtgtaccatt ctttgctcct aagtcaccac cggataccgg tgcatctaac gcatctgcgc
    29161 ctttttcttt tgctgttttt gcaatttttt ctgccaggct tggtgtactt gtagtcaaat
    29221 caactaaaat atgtcctgag atatctgcag aaaaaatccc cgactgtcca tagtagattt
    29281 cttctacatc tttggggtaa cctaccattg taaagagaat attactttgt tctgctaccg
    29341 cttttggtgt atctgcccag atagctcctt ctgttactaa aggatcagct tttgcttttg
    29401 tccgattata gacagctact tcgtgtcctg cttctagtag atggcgtgcg acagcacttc
    29461 ccatgacgcc tgttccgata aaaccaagtt tcattttttc gacctcctaa aaatcaaaaa
    29521 tccttgcttc tatgctagta tacgctagaa acaaggattt tttcataaaa aatgtttgaa
    29581 ttttatcttt taaatgaatt taattttatt ttgattcgtt caatcgagtc ttcttcttct
    29641 tttcttcgca tctcttcttc atgataaaca accatgtctt gataaaattc gatcatagct
    29701 tcgccgaaat gtgtagatga aatatcataa agtttagctt ctaaaacttt tggatcttta
    29761 ggaacttcac tggaaatata atcaataagt gctggtgcaa agtcttcgtc ggtttcaaaa
    29821 gttttcccta atgaaggatg atcgaacaat cggttcaaat aagcatttcc ttccacgaca
    29881 caaggtgttc cggctgccat cgcttctgta taagtaagcc cttgtgtttc tgaagtagaa
    29941 gcactgacaa aataatctgc cgctttataa taaagtgcta catcattgtt gtccacttct
    30001 cctgtgaact gcacaaattc ttctaactgc agttctgaga tcaattcctc taactcttct
    30061 acataaggtc cttttcctac gataactaat ctggcgttag gataagcgga gatgacctga
    30121 ggaaacccat taatcaaggc ttggatattt ttctcataag aaatcctgct taaagaaaga
    30181 atcatcaaat ggttttcctg aagacctagt gactccctta atttgctgat atcctgctga
    30241 gtgatatcag aacgtttgaa tttttcaatg tcgataccag taggaatcac gcgaagcggt
    30301 gctattacac catagctgcg taatgtatca ataacacgtt cacttggaca aactacacct
    30361 gtcgtatgat ttgcaaataa tctagacaag tattttacat gggattgcct gatcactttt
    30421 cctttaccga tataatgaag gtagtcttca tacatcgtat ggtaagtatg gatcagaggt
    30481 attttcaact tcttagctac catcttccca agtatccctg ctccgaattc tgtatgggta
    30541 tgaataagat ctagttctag ttctttggca accaaataag cataccacat gcctctaaca
    30601 acaactcttc gatccttgaa agaaataaat ggtacacttg gcattcgaat cacatctttt
    30661 tccaacttat ccgcattagg atcggtcgtt gtaaaaatat agacttcatg cccatgtctt
    30721 tctaattctt gttttaatgt tttgatcgat gtagcaactc cgctgacttg cggaaagtag
    30781 gtatcggtaa aaaagccgat tttcatgttt ttcgcctcca aactagtcct tgacctgatt
    30841 tacttttttt aatttgctag agactttttc attcagtaca ctttcgtata catttttaag
    30901 ttcttcaccg attttattga tacttcgctc ttttgctgtt tgataaccgg cttgtgtcgt
    30961 actcggtagt tcttgattgg taatctgttg gatcagtcgg ataaattctt cgtttgtctt
    31021 ccccatatag cagttcttgc tatcttctaa ccagccatta tacactggaa tatctcgaac
    31081 aagtacgttt tgtttgcttg ctaaagcttc taaaacaacg atcccttctg tctcttcata
    31141 agaagggaaa aagaatagat ctgcacctga atacgcacct tctatgatct ctccacgaat
    31201 gtaaccagga aacaggacat tttcaggatg atcttctttt acaattttcc ggatgttccg
    31261 aggaatcgag tacatgggaa catgaccaaa ccaaataaat gtgtattcgg gcatcttctt
    31321 agcaatttct acgaaatcaa tgattccttt acgttcgaaa aacaaaccga cacaaatgat
    31381 tactttttga tccggagaca gtttgaaata ttctctgaac ttttgttctt tcattggatc
    31441 agggtgatat ttctctaagt cgatcccatt ggaaatagct tgaatcggca cacggatacc
    31501 atagctttcc aacaactgtt ttgaataagg tgttggcgtg atcagatagt ccgctttttg
    31561 gtacaatcct accagatatt tttttacgat aggagaaagc tggttcgatc cgataaagga
    31621 atttcggaaa tcttcctctg tagaatgcgc atgatagaca actttttttc cttctcttct
    31681 ggctttattg accatattat gactattgat tccgtatgta ttgatgtgca ggatgtcata
    31741 atcttctttt tcatctagtg tataagagat accgacttcc gtcagagcac gtttttgatg
    31801 atcaagcgca cgcccgattc cagattttgc aagcatcttt tcactttcaa aatataataa
    31861 taccttcacc ttacgtcccc cttcacttat atagtataac atagcctaca aactgtcact
    31921 caaacttaag ccctattttt gtctattctt actgacaaaa acgttttctt aaaaaagaac
    31981 aaaaaaactg gcgtagctct tttacaagcc acaccagctt ttgatacgtt gtttatttta
    32041 catattcatg aacaagttca acgacttctt ccatcatatc gcattctttc aatgcacgat
    32101 cagcaagttc agccattttc gctgtgtcca agcgtttcat taaactacgc gttttaagaa
    32161 tagatgtcgc actcattgag aactcatcta gacccatacc tactagcaat ggaacagctg
    32221 tttgatctcc agccatttct ccacacatac ctgcccattt tccttcagcg tgtgctgcat
    32281 caattacatt tttgatcaaa cgtaaaattg atgggttata tggttggtac aagtaagaaa
    32341 cgcgttcgtt catacggtct gctgccattg tgtattgaat caaatcgttc gtacctacag
    32401 agaagaaatc tacttctttt gcaaacttgt ctgctaagac tgctgctgct ggaatctcga
    32461 tcatgatgcc gacttggatc gtatcagata cttccttgcc ttcacttatc aatttttgtt
    32521 tttcttcttc aaagatcgct tttgctgcgc ggaattcttt gagtgtagct accataggga
    32581 acatgatacg taaattacca tgaacagatg cacgaagcaa tgcacgcatt tgtgtacgga
    32641 acatttcgtc gccttgttca gataaactga tacgcaatgc acgatatcct aagaatggat
    32701 tcatttcatg aggtaattgc agatatggta gttctttatc tccaccgatg tccattgtac
    32761 gaacaactac aggttttcct tccatacctt ctaatacagc tttgtaagct tcatattggt
    32821 catcttctgt tgggaagtct ggagaatcca tatacaggaa ttcagtacgg tacagaccaa
    32881 ctgcttctcc accattatta tggacaccaa ctagatcttt tggtgttccg atgtttgcag
    32941 ctaattcaaa atgcttgccg tcagctgtga cagtttctgc attttttaat ttttcccatt
    33001 ctgctttttg tgctgcgtaa tctgcacctg ctttttcaaa ttcagctttt tgttcatctg
    33061 taggatcgat gattacatcg ccttcgatcc cgttgacagc caaaatatcg ccttctttga
    33121 ctttagcagt aatttctttt gtcccaacga ttgctggaat ttctaaagaa cgtgccatga
    33181 tagcagaatg tgaagtacgt ccaccaatgt ctgttacaaa cgcttttaca aaattgcgat
    33241 ctaattgtgc tgtatcactt ggagtcaagt catgtgcaac aacgaccact tcttcgttga
    33301 tcattgatgg atttggtaaa gtaacgccta ataagtgcgc taaaatacgt ttagcaacgt
    33361 cacgaatatc tgcagcacgc tcttgcatat aagcattatc ttccattgct tcgaacatac
    33421 cgatatacat atccgttact tcttttagcg ctgattcagc atttacgctg ttgtctttaa
    33481 tattttgctt gatttgacct accatttctg gatctgaaag aaccatcaaa tgggcatcaa
    33541 atacttgcgc ttctgcttca cctaagcttt gcgctgcttt ttcacgaatt tgctgcaatt
    33601 cttcagtaga tttagctaat gcagcatcca aacgagcttc ttcagccgca gtatcttcta
    33661 ctgttgcctt actgaatgtt aaatccggtt gaactagcag gtaagctttt gcaacggcta
    33721 caccgtcact agcggcaatc ccttttagca tttcaaccat tattcagata agccttcttt
    33781 cttcattgtt tcaacgattg ctgccattgc atcagcttcg tcagcacctt ctgcagtaat
    33841 agtaacatca gaaccttgac ctacacctaa agacataacg cccatgattg atttcaagtt
    33901 tactgattta cctttatact ctaagtttac atcagagtta aatttgcttg ctgtttgtac
    33961 taatagtgta gctggacgag cgtggatccc agtttctgct actacgtgaa attctttctt
    34021 ttccatatta atcggtctcc tttaagtgag tgttgttttt atgtgagggt tttctttttt
    34081 agtaattcag agaatcctaa caaatgccac cctttcatct gtaaagatta acattttttt
    34141 tcgttagata caacagtttt cattcaattt cagataaaag tattcaaatt attccggatt
    34201 ctttttcatc cttttcttca cattttataa caaacgtgtt tatttccttt ttcttgtcgc
    34261 tttctcttct cctgacgttt atcattcttt cctttgaccc tctttgtttt catatgaaac
    34321 gaattacttg cttccctttt tatttttagt ataataagag tcaaaggtca ggaatggtca
    34381 atagagattt caccttttct acctcataaa tctcttaaaa aactaggggt tattctttta
    34441 cctcatgaaa aacgagatgt aaaatatgga tgtacccatt tttgtgaaag gacgtgaatg
    34501 attcatgctt tgtcaaaact gcggcaagaa tgaggcaacg attcacttgt atgccactgt
    34561 caatggtcag cggacacaac tcgattactg tcagagctgt tatcaaaaaa tcaaaaatca
    34621 acaaaatgga ggtctgacta atatggccca aaatgatcca tttggatttg gcagtttgga
    34681 cgatcttttc cgttccatgt ctcgccaaat gcaacagcaa ggaaattatg agcaaacacc
    34741 accaacacaa tttggcggaa acaacaactt caatggtggt cagccacctc aaggcaatac
    34801 agaaggttta ttgggagaat acggaatcaa tatcacagaa caagcacgac aaggtgatat
    34861 cgatcctgtg atcggacgcg atgacgaaat caaacgagtc attgaaattt taaatcgccg
    34921 aaccaagaac aatcctgtct tgattggaga accaggtgtt ggtaaaacag cagttgttga
    34981 aggtttagca caaaaaatcg tagatggtga tgtaccgcaa aaattaatgg acaaagaagt
    35041 cattcgttta gatgtcgtat ctttagtcca aggtactggt atacgaggac aatttgaaga
    35101 acgtatgcaa aaattgattg aagagatccg tcaagcagaa aatgtgatcc tattcataga
    35161 tgaagttcac gaaatcgtcg gtgctggttc tgctggtgat ggaaatatgg atgctggtaa
    35221 tattttaaaa ccagctttag ctcgaggaga attgcaaatg gttggtgcta ctaccttaaa
    35281 tgagtaccgc atcatcgaaa aagacgctgc tttagaacgt cgtatgcaac ctgtacgggt
    35341 agatgaacca actgtcgaag aaacgatcag tattttgaaa ggtttgcaaa aacgttatga
    35401 agactatcat catgtaaaat atactgatga ggccatcgaa gcagccgcaa ctttatccaa
    35461 tcgttacgtc caagatcgct tcttgccgga taaagccatc gatttgctag atgaaacagg
    35521 ttctaagaaa aacttgacga tccaaatcgt tgatcctaaa acaatcgaga aaaaactgca
    35581 agaagcagaa gaacaaaaag ttttagcttc tcgagaagaa gatttcgaaa aagcagctta
    35641 ttatcgcgat cagatcaata aacttcaaaa aatgaaagaa cgtcagctca ctgaagagga
    35701 aacacctgtg attaccgaaa aagatatgga aaaaatcgtt gaacaacgga caggtattcc
    35761 agtgggtgaa ttaaaagaaa aagaacagac acaactgaaa aacttagcag atgacttgaa
    35821 agctcatgtc atcggacaag ataacgctgt agatcgagta gcgaaagcga ttcgccgaaa
    35881 ccgtgttgga ttaaacaaac agaatcgtcc aatcggctcc ttcttatttg ttggaccaac
    35941 tggtgtaggt aaaacagagc ttgcgaaaca acttgcctac gaactattcg gttcacaaga
    36001 ttcaatgatt cgtttcgata tgtctgaata tatggaaaaa catagtgtct ctaaattgat
    36061 tggttctcct ccaggttatg tcggatacga agaagctggt caattaacag aaaaagtacg
    36121 gagaaatcct tacagtcttg tcttgttaga tgaagtagaa aaagctcatc cagatgtttt
    36181 gcatatgttc ttgcaaatct tagatgatgg tcgattgaca gatgcacaag gaagaacggt
    36241 cagctttaaa gatacgatca tcattatgac aagtaatgcc ggaactggta aagtagaagc
    36301 aaacgttggt tttggtgcgg cacgcgaagg tgttactcgt tctgtattga accaattaaa
    36361 caactacttt acaccagaat ttctgaaccg ttttgacggg atcatcgaat tcagtgcatt
    36421 atcaaaagaa aacttgatga caatcgtcac actaatgcta gacgatgtca accaaatgct
    36481 ggcagcacag caacttcaca tcgaagttcc tacgaatgtc aaagaaaaat tagttgactt
    36541 aggctatgat ccatctatgg gtgctcgtcc tcttcgacgc acgatccaag aacaaatcga
    36601 agatggtatc gctgaatttt atcttgatca cccttctatc catgagttga aagcaaaact
    36661 ggataaagat ggaaaaatca tcgttacttc taaaccagaa cgtttggcta aagaatcagc
    36721 cgaagaaacg gctgaataaa tccagtttta tagcgtgaga cggtatttct ttgagaaatg
    36781 ccgtctcatt cttttttcag aaaagtcaaa atactgttca ttttataaaa aagaaattat
    36841 aataagagaa acaagcaatt aaaaatacat aaaagaggtg agcgccatga aattggtaaa
    36901 tgtaacaaac agccattccc gtcttgtatt aaaccagctt gaaaacactg atgcccatct
    36961 agtcaaggtc tacacagccg gaaataccac tattgtctac actgaagccc ctgaacataa
    37021 tgaaattctg ctgatcaatg acaaacgtaa aatccagccg aaagaaatcg aagatgctaa
    37081 aaattatttc ttagataaaa tcaaagacgc cgtttaccac gaggatgaaa tcaaagtaat
    37141 cgaattggca ggccttgttg aaatctcgat tccaaaacat gaattaggcg ctacattagc
    37201 cgtgtaaaca aattttgaac ataataatta aagagcgtgg gacaaaagta aagaacactt
    37261 ttgtctcacg ctctaaacac gtataaacgg cgaaagcaga agcaactcct tcggaaataa
    37321 gctgaaattc acgaaaattg cttttcaaat ttttgtgaat tccagcttat ttcttggagt
    37381 taaacgcttc tgtctcagcc tctttttctt tctagctttt tgatttttct cttttctttt
    37441 gtacttttct cttttcattt tcagttctac gcgtgtcttt atttttaact ttttcctctt
    37501 tatggatgat tgatcgtact ttttgtgcaa tttgctcgtt tttgtcccag tcttttacat
    37561 ctaataatcg acataatcgt aacatctctt cttttttctg tttttttctt tcacgtcttt
    37621 gccattctgt ttccatgtgt cacactctcc tttctttaat catatgtacg caaaataatt
    37681 tttatatttt tttgattaga catttctatg attttaatat agtaaaaaga gccgtcacgg
    37741 aaaatccgtc acggctctaa acaaaatatg agaggctgtt tttgtttggc tattcttatt
    37801 acttgatgca gggcagcttg tgtcaagact tctttacatc aaacttttta attttacatt
    37861 gggatattta tccgcaaacc agcgttctgc aaattggttc tcgaataaga acaacggttg
    37921 gtcgaaacgg tcacgagcca agatattgcg acttgaactc atcttctcat ccaaatcttc
    37981 tggatcgatc cagcgagcga ttttgtgtcc cattggagac atgattactt cggcgttata
    38041 ctcatttaac atacgatatt ggaatacttc gaattgtagc tgaccaacag ctccaatgat
    38101 atattcttcc gttagatatg ttttataaag ctgaatcgct ccttcttgaa ctaattgata
    38161 gattcctttg tggaaggatt tttgtttcat gacatttttt gcagtcactt tcataaacag
    38221 ctctggagta aatgaaggga gttcttcata agcaactttt agttttcctt cgaacaatgt
    38281 atccccaatc tgataattgc ctgtatcata tacaccgatg atatcgcctg ctactgcttt
    38341 ttcgacgttt tctctcgaat ctgccataaa ttgtgtgaca ttgcttaatt tcagtttttt
    38401 atttgtacgt tccaaccaga catccattcc tcgttcgaat gttccagaac agattcggac
    38461 aaaagcgatt cggtcacgat gggctggatt catatttgct tggatcttga atacgaaacc
    38521 tgaaaattct tcctcataag gacttaccat ctgatcatct tgtgttttgt gtccataagg
    38581 agctggtgcc aattcaacga aagtctctaa aaaagtctgt acaccgaagt tagtcaatgc
    38641 agaaccaaaa aatactggtg tctgatcgcc acgagcaatt ttttcttgat taaatgcatc
    38701 tccagcctct ttgattagtt ctacttcttc taagacttgt tcatataagg aattttcagt
    38761 tagaggatga ttttcgggaa gcagtccatc ttcatccaat tctactaatc gttcatcctg
    38821 ataattttcc ggacgataaa actctacacg attatggtag atatcataaa gaccttctag
    38881 ccctttaccc ataccgattg gccaattcat cggataagat tcgatatcta atagctcttc
    38941 taattcttct aacaattcca atggttcacg accatcacga tctagtttgt tgataaaagt
    39001 aaatatcggg atgccacgtt tttttacaac ttggaataat ttctttgtct gtgcttcgat
    39061 cccttttgca ctatcgatta ccatgaccgc actatccacg gccatcaaag tacgataagt
    39121 atcttcagaa aaatcttcat gtcctggtgt atccaagata tttactcgtt tcccttgata
    39181 atcaaactgc ataacagaac tagtaacaga aatcccacgt tgtttctcga tttccatcca
    39241 gtctgatttg gcaaaattac ctgttttttt gccttttacg gtcccggctt gacggatagc
    39301 cccaccaaac aatagtaatt gttctgtaat cgttgttttc ccagcatccg gatgggaaat
    39361 gatcgcaaaa gttctgcgat tgtccacttg ttgctttaat tttggattat tcataaccta
    39421 actccatttc ttccattcaa ttatttatat tttctctagg cacagattgt ccgatctctt
    39481 aaagaaagac aatttcatat agggctgtga caaaagccgt gtagcagtct ctttttccga
    39541 gtaaacggcg ttctagagac agcctatcag caataagccc acccaagcca aaaatacgaa
    39601 aagctgtttt tgcttggctg ctcttattac ttaatgcagg atagcttttt cacaaccttt
    39661 gtcaactact acaactgaat cagtatagca tggattcagc gaatgaaaaa gccaccaaac
    39721 cataagagtc aaatattttt ttgtcagctg actaaaagaa aagacgtgtt gcttgtttat
    39781 tcaagcaaca cgtctttcat aaccaagttt ccaattatct tcttggctcg tcatcttcga
    39841 attcttttct gaagtatctt cgttcttctt caggttccgc ttctgtttct tccggatcat
    39901 gaaaaatgac acggatttta aggattcgag aaccttccat ttcttcagaa accagcgtaa
    39961 tattatcgac atcaaacgat aacttttcgc cttcatccgg aatcatgcct aatgctgtaa
    40021 tcaaataacc agccatcgta tctacatcac tcatatgcag atcactgtca aaagcttcat
    40081 tgaactcatc aatcagcata cgtccttgga tgatatattc gtgttcatcg attttttcat
    40141 aaagattctc aacttcatct gattcatcat cgatttctcc gacgatttct tctaaaaggt
    40201 cttctagtgt tgctaaccca acgacgccgc catattcatc taataaaata gccatctgat
    40261 tctgcgtctt tttcatttca tataacagat catcaataaa gaccgtttca ggaacgaata
    40321 aaggctcctg catgattttc ttgatatcta aattatcaaa gccgaattta tgggcagctt
    40381 ttagtaaatt ctttgtatga aggattccga ctactttatc tttgtcttcg ttataaacag
    40441 ggattcttga atagttttcg gataaaactt cattcacatt ttcttccaca gtgtcgttga
    40501 tatcaatcat aaatgcatcc gttcgtggga ccatgacttc tcgggcaact ttcgtatcaa
    40561 gagaaaaaac cccttggagc atttctaatt cttcattatt caatacgcct tcgttttcaa
    40621 gcatataacg catttcatct ctggtcattt ttgaatcttc atcatcaaaa gtcatcggcg
    40681 tgatacgcgc caataaactt gtggaagcag aaagcagcca gacgaagggc ttagctacta
    40741 caccaatcaa gcgtatcagc cctgaagtga actttgcgac ttcttctgat ttatttaaag
    40801 caatacgttt agggtacaat tctccaaaaa caatcgatac ataggtcaaa atagccagca
    40861 caatgatatt tgcaatattt tttgccgctg tgccgccacc taataccgga gctaaccgtt
    40921 ccgataaagt attagctagt gaagcaccag acaaaatgtt gaccaaagtg attcctactt
    40981 ggattgtcga taggaaatta tttggattct gcaatatttt taaaagcttt tgagatttca
    41041 catctccttc atcagctttt tgctcgattc gattcttatt gatcgaaaca acagcaattt
    41101 cggaagctgc taaaaaagca ttgatcaatg tcaaaacgat caataataaa atctgcgcta
    41161 acagcgactg actctcaggg tcagcattca taatcgcatt ctctccttat aaaattaatt
    41221 tttagtttac gatagaaaaa aacgtggcac tatagccacg ttaaaatggt atcacaaagc
    41281 aattttcttt gcaataaaaa caagtgtact aaaactacct acgaggtcgt agcaattttc
    41341 ttcttgtttt cattttgctt gtcttctttg acgattttga ttatatagga aatgatcgtc
    41401 ttgcagattg cgtaaaaagg aacacctaag aagataccaa gcaatccggc aatatttcca
    41461 gctactagaa gaacgataat gatcgttaat ggatggattt tcagtgattt cccaattaca
    41521 ttaggataaa tgatattccc atccaactgt tggacgatta gcacaaccac acaacaaagt
    41581 aatgctttta ccggatcatt gaagactgtt actagaaatg caggtgctag tcctagataa
    41641 ggtcctaaat atggaattag gtttgtcaat cccgcaatga ccccaaataa gaaagcataa
    41701 tccacaccaa ttaccatata tcctaaaaat gtgaaggtac caacgaataa acattcgatt
    41761 gcttgtccgc taatgtaatt agccaatgtt ttattcagtt gccccaataa ctccacaatt
    41821 tggtttcttc ttttttctgg gaaaaactgt tggatgtttg gcactagttt ttctccgtct
    41881 ttcaacatat agaacaagat gaatggtgct gtaatgatta ctacggttgc cgaagcgatc
    41941 gttccaacga tcgaacctaa actattggat actccactaa ggaattgctg aatgattgtc
    42001 ccgtaagaaa tgtctaattg ctcaaaatat ttattgatat ctacttcctt gaacaaaggc
    42061 aattcggcta cctcttttaa ccagctttct acctgtttga tgaaatcagg catattagat
    42121 gccaatgagg aaatttgctg tactagactt gggatgacac taagtaaaat ccaaataatt
    42181 gctgcaacta aaaggagcag cacaattgcg acagcccata ttcttttgat ttttgctttc
    42241 atcaacagct tgacaatagg attcaataaa tagtaaagaa agcctgcaat cagtacaggt
    42301 gcaaataacg tggaaaaaaa tgtcccgatc ggttcgaaaa taaagtttat ttctgatgaa
    42361 atcatgatca atgcagctat gatcaataat tcaagcgacc aaaacattag tttagactgt
    42421 ctaagttttt caaacaatag atttcctcct ttctttctta ctagtatata ttatattcgt
    42481 taaaaattct tttattcttt tttt
//
LOCUS       DACDIE010000017        42252 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.017, whole genome shotgun sequence.
ACCESSION   DACDIE010000017 DACDIE010000000
VERSION     DACDIE010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42252
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.017"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            13..237
                     /gene="nrdH"
                     /locus_tag="IV250_001031"
     CDS             13..237
                     /gene="nrdH"
                     /locus_tag="IV250_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAP8768331.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            396..2555
                     /gene="nrdE"
                     /locus_tag="IV250_001032"
     CDS             396..2555
                     /gene="nrdE"
                     /locus_tag="IV250_001032"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAP8768332.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            2605..3570
                     /gene="nrdF"
                     /locus_tag="IV250_001033"
     CDS             2605..3570
                     /gene="nrdF"
                     /locus_tag="IV250_001033"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAP8768333.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(3659..4798)
                     /locus_tag="IV250_001034"
     CDS             complement(3659..4798)
                     /locus_tag="IV250_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP8768334.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(5107..5820)
                     /locus_tag="IV250_001035"
     CDS             complement(5107..5820)
                     /locus_tag="IV250_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAP8768335.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(6074..6775)
                     /gene="nagB"
                     /locus_tag="IV250_001036"
     CDS             complement(6074..6775)
                     /gene="nagB"
                     /locus_tag="IV250_001036"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAP8768336.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            7009..8541
                     /locus_tag="IV250_001037"
     CDS             7009..8541
                     /locus_tag="IV250_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP8768337.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(8732..9934)
                     /locus_tag="IV250_001038"
     CDS             complement(8732..9934)
                     /locus_tag="IV250_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8768338.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(10016..10705)
                     /locus_tag="IV250_001039"
     CDS             complement(10016..10705)
                     /locus_tag="IV250_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP8768339.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(10811..11179)
                     /gene="rplL"
                     /locus_tag="IV250_001040"
     CDS             complement(10811..11179)
                     /gene="rplL"
                     /locus_tag="IV250_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAP8768340.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(11232..11735)
                     /locus_tag="IV250_001041"
     CDS             complement(11232..11735)
                     /locus_tag="IV250_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAP8768341.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            complement(12145..12834)
                     /gene="rplA"
                     /locus_tag="IV250_001042"
     CDS             complement(12145..12834)
                     /gene="rplA"
                     /locus_tag="IV250_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAP8768342.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(12938..13360)
                     /gene="rplK"
                     /locus_tag="IV250_001043"
     CDS             complement(12938..13360)
                     /gene="rplK"
                     /locus_tag="IV250_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAP8768343.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            13703..14371
                     /gene="sdaAB"
                     /locus_tag="IV250_001044"
     CDS             13703..14371
                     /gene="sdaAB"
                     /locus_tag="IV250_001044"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HAP8768344.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            14384..15256
                     /gene="sdaAA"
                     /locus_tag="IV250_001045"
     CDS             14384..15256
                     /gene="sdaAA"
                     /locus_tag="IV250_001045"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HAP8768345.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(15347..16366)
                     /locus_tag="IV250_001046"
     CDS             complement(15347..16366)
                     /locus_tag="IV250_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP8768346.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(16539..17426)
                     /locus_tag="IV250_001047"
     CDS             complement(16539..17426)
                     /locus_tag="IV250_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP8768347.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(17503..18048)
                     /gene="nusG"
                     /locus_tag="IV250_001048"
     CDS             complement(17503..18048)
                     /gene="nusG"
                     /locus_tag="IV250_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAP8768348.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(18149..18319)
                     /gene="secE"
                     /locus_tag="IV250_001049"
     CDS             complement(18149..18319)
                     /gene="secE"
                     /locus_tag="IV250_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAP8768349.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(18343..18495)
                     /gene="rpmG"
                     /locus_tag="IV250_001050"
     CDS             complement(18343..18495)
                     /gene="rpmG"
                     /locus_tag="IV250_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP8768350.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            18657..19301
                     /locus_tag="IV250_001051"
     CDS             18657..19301
                     /locus_tag="IV250_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP8768351.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(19357..19860)
                     /locus_tag="IV250_001052"
     CDS             complement(19357..19860)
                     /locus_tag="IV250_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP8768352.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(19873..19919)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            20457..21422
                     /locus_tag="IV250_001053"
     CDS             20457..21422
                     /locus_tag="IV250_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP8768353.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(21521..22693)
                     /locus_tag="IV250_001054"
     CDS             complement(21521..22693)
                     /locus_tag="IV250_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8768354.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(22680..23825)
                     /locus_tag="IV250_001055"
     CDS             complement(22680..23825)
                     /locus_tag="IV250_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8768355.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(23839..24763)
                     /locus_tag="IV250_001056"
                     /pseudo
     CDS             complement(23839..24763)
                     /locus_tag="IV250_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            24880..25959
                     /locus_tag="IV250_001057"
     CDS             24880..25959
                     /locus_tag="IV250_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP8768356.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            25991..26584
                     /locus_tag="IV250_001058"
     CDS             25991..26584
                     /locus_tag="IV250_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8768357.1"
                     /translation="MTKSLQTILESTKEIRVLGIGHCAKDALVLYETHRPDVLLTDIR
                     MPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYLIKQNLQAIIPSV
                     KSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKEVAAGKNNKEIAD
                     ALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(26694..26921)
                     /locus_tag="IV250_001059"
     CDS             complement(26694..26921)
                     /locus_tag="IV250_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP8768358.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(27024..29612)
                     /locus_tag="IV250_001060"
     CDS             complement(27024..29612)
                     /locus_tag="IV250_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAP8768359.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(29632..30291)
                     /locus_tag="IV250_001061"
     CDS             complement(29632..30291)
                     /locus_tag="IV250_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP8768360.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(30340..30843)
                     /gene="trmL"
                     /locus_tag="IV250_001062"
     CDS             complement(30340..30843)
                     /gene="trmL"
                     /locus_tag="IV250_001062"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAP8768361.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(31064..31930)
                     /locus_tag="IV250_001063"
     CDS             complement(31064..31930)
                     /locus_tag="IV250_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP8768362.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            32039..32680
                     /locus_tag="IV250_001064"
     CDS             32039..32680
                     /locus_tag="IV250_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAP8768363.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(32742..34103)
                     /gene="mgtE"
                     /locus_tag="IV250_001065"
     CDS             complement(32742..34103)
                     /gene="mgtE"
                     /locus_tag="IV250_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAP8768364.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(34121..35020)
                     /locus_tag="IV250_001066"
     CDS             complement(34121..35020)
                     /locus_tag="IV250_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP8768365.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(35022..35819)
                     /locus_tag="IV250_001067"
     CDS             complement(35022..35819)
                     /locus_tag="IV250_001067"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HAP8768366.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(35797..36501)
                     /locus_tag="IV250_001068"
     CDS             complement(35797..36501)
                     /locus_tag="IV250_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAP8768367.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            36632..37207
                     /locus_tag="IV250_001069"
     CDS             36632..37207
                     /locus_tag="IV250_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HAP8768368.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            37325..38002
                     /locus_tag="IV250_001070"
     CDS             37325..38002
                     /locus_tag="IV250_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP8768369.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(38081..39889)
                     /gene="pepF"
                     /locus_tag="IV250_001071"
     CDS             complement(38081..39889)
                     /gene="pepF"
                     /locus_tag="IV250_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAP8768370.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSDAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(40065..41063)
                     /locus_tag="IV250_001072"
     CDS             complement(40065..41063)
                     /locus_tag="IV250_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAP8768371.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(41289..41942)
                     /locus_tag="IV250_001073"
     CDS             complement(41289..41942)
                     /locus_tag="IV250_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HAP8768372.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT        12770 a         8799 c         7465 g        13218 t
ORIGIN      
        1 gaggcactca ccatgaacgt aaaaatcttt tctaaaaata attgtatgca atgtaagatg
       61 gtcaaacggt ttttaacaga aaatcaaatt gcttttgaag aagtcaatat cgatgaacag
      121 cctgaagcag tagactggtt gaaagcacaa ggattccaaa gtgttcctgt cattacttct
      181 gatgcagaaa cagttatcgg attccgtcct gatcagttgc gtaaacttgc cagctaacat
      241 gactaaaaga atcaaaacgt gatacatgga tttgaagggc ttgagacagc accagttttt
      301 gtcgcaaact tccaaatcct tttttaaagc ttaaaaacaa tgcaatgatt tgcaatcaag
      361 catcatcaaa tacgaaatga aggttgggcg tacgtatgag tcttaaaaat ttacaagatg
      421 tcagttattt caagctgaat aatgaaatca atcggccagt caatggtcaa atccctttga
      481 ataaggataa agaagcttta gttgcctttt ttgaggaaaa tgtcaaacca aatacgatga
      541 cttttctatc tattatggaa aaaatcaatt acttgatcga tcaagaatat atagaagcag
      601 aattcattca attgtattca ccatcattta ttgaaaagct atatcaattt ttggacgagc
      661 aacatttttc tttcaaatct tttatggctg cttacaaatt ttattctcaa tatgcgttga
      721 aaaccaacga cggttccgct tatctggaaa cttatgaaga tcgagtagcc ttcaatgcgc
      781 tttactttgc gaacggggat gaagaattag cacttgctct tgcagacgaa atgatccatc
      841 aacgatatca gccagctaca ccttctttct tgaatgctgg acgtaaacgt cggggcgaat
      901 tggtgtcttg tttcttgact caagtcactg acgatatgaa ctctatcggg cgctcaatca
      961 actctgcttt gcaattgtcc cgtattggtg gcggcgtagg gattacttta tcaaatcttc
     1021 gtgaagccgg cgctcctatc aaaggatatg agggtgctgc aagtggtgtc gttccagtca
     1081 tgaaattatt cgaagatagt ttcagctatt ctaatcaatt aggtcagcgt caaggagctg
     1141 gcgtggttta tttgaacgtt ttccatccag acattattgc tttcctttca gcaaaaaaag
     1201 aaaatgcaga tgaaaagatt cgcgtaaaaa ctctttctct tggcgtgatc gtacctgata
     1261 aattttatga attgacacgt aaaaacgaag atatgtattt atttagtcca tatagtgtcg
     1321 aaaaagaata tggtgtccca ttctcttatg taaatattac agctgagtat gatcatctag
     1381 ttgcaaatcc aaacattcgc aaacaaaaaa tcaaagcgcg tgatcttgaa aatgaaatct
     1441 caaaattaca acaagaatct ggctatcctt acgtaatcaa tattgataca gcgaaccgcg
     1501 agaatccaat cgatggcaag atcatcatga gtaatttatg ttcagagatt ttacaagttc
     1561 aaacgccttc tgtgattaac ggcaaacagg aatatgaagt attaggaacg gatatctcat
     1621 gtaacttagg ctcgacaaat atcgtgaatt tgatgcaaag ccctaacttt ggtcaatctg
     1681 tgcgtacgat gactcgtgca ttgacattta ttacagatgc atctgatatc gatgtcgtac
     1741 caccgatcca aaacggaaat aagctgaatc atacgattgg attaggtgcc atgggcttac
     1801 acacttatct tgcaaaagaa caaatggaat atggctcaga ggattcatta gactttacaa
     1861 atatttactt catgctgttg aattattgga cacttgttga aagcaacaat atcgctcgtg
     1921 aaagaaaaca agttttccat aattttgaaa aatcagctta tgctgacggc acttatttcg
     1981 aaaaatacgt aactggagaa ttccagccaa aatcagaaaa agtcaaagca ttgtttgaag
     2041 gcatttttat cccaactgct gaagattgga atgctttgaa acaagcagta atgaaagatg
     2101 gactgtatca tcaaaatcgc ttggcagtag caccaaatgg ctcaatttca tatatcaacg
     2161 atacgagtgc aagtattcat cctatcactc gcatgatcga agaacgccaa gagaagaaaa
     2221 tcggtaaaat ttattaccca gcgccttatc tatccaatga gacgattccg tattacactt
     2281 ctgcttatga tatggacatg cgtaaagtaa tcgatgttta tgcaatggca caacaacacg
     2341 tcgatcaagg catgagtttg actttattca tgcgttctga aatcccagaa ggcatttatg
     2401 aatggaagac aacaacgaag caaacgacac gtgatttgaa tattttaaga cattacgctt
     2461 tccacaaagg aatcaaatcc atttattacg tacgtacctt tactgatgat gcagaagaaa
     2521 tcggaagcaa tcaatgtgaa agctgcgtga tctaatatca tagatagatt cgcttattca
     2581 tattttaatt aaaggagcat tctcatgtca gaaacatatt atgaagcaat aaactggaat
     2641 gaaattgaag atatcattga taaatccact tgggagaagc tgacagagca attttggcta
     2701 gatacacgta tcccactttc aaatgaccta gatgattggc gtacattgtc tcaattggaa
     2761 aaagatacag taggacatgt atttggcggt ttgacactgc tagatactgt ccaatctgaa
     2821 agtggcatgg atcagttgcg taaagatgtc cgcactcccc atgaagaagc cgttttgaac
     2881 aatattcaat tcatggaatc cgttcatgcc aaaagttatt cttctatttt tagtacactg
     2941 aatacgaaaa aagaaatcga agaaattttt gactggacaa atacgaatcc ttatctgcaa
     3001 aaaaaagctg agcgtatcaa tgaaatctac aaacacggta caccgctaga aaagaaaatt
     3061 gccagcgtct ttttagaaac attcttgttc tattcaggtt tttatactcc tctttattat
     3121 ttaggaaaca acaaattggc taacgtagca gagatcatca aactaatcat ccgagatgaa
     3181 tctgtacatg gaacctacat cggttacaaa ttccaattag gattcaacga gttacctgaa
     3241 gcagaacaag aaacgttaaa agactggatg tacaatcttc tttatgagct ttacgaaaac
     3301 gaagaacgtt atacagaaga attatacgat ccaatcggct ggacagaaga agtcaagaca
     3361 ttcctacgct ataatgccaa caaagcattg atgaacctag ggatggatcc actctttcca
     3421 gatactgcca atgatgtcaa cccaatcgta atgaacggga tttcaacagg cacaagcaac
     3481 catgacttct tctcacaagt cggaaacggc tatttgttag gtcatgtaga agcgatgaaa
     3541 gacgacgatt atttgattgg attggaataa ggatttattt taattcaaaa atttttcgaa
     3601 aaataaaaag agtttagcgt acagatattt taaatgtaca ctgaactctt ttttatgctt
     3661 attcacttgt ttcttccagc acttttggac tgtctaactt ttccgaaaca tgttccattc
     3721 ttctcaataa atagattttc cggatattta atattatcgt cttaatcact gcatagacag
     3781 gtatgcaaat cagcatgcct aatattcctg cgacacttcc tgctccaatc aataccaaga
     3841 tgatcgtcaa aggatggata ttcattgatt tcccaaataa caacggcttg atcaaatttc
     3901 catctaattg ttggatcact aaaatcgaca acgccatata caaagcttga aatggagaga
     3961 taaacaaacc gacaatgatt gctggtaccg ctccaataaa agggccgaca tacgggataa
     4021 tattcgtgat tccgcaaatg accgcaagca acaagctata aggctgatcg aatattctca
     4081 ttgccaaaaa gctcattaca ccaataatca aagcatctaa gatcgtactg ctgatatagg
     4141 cagataatgt ttcgtttaat tcttttaccg tttggcgtaa ctctttacga atcgctttcg
     4201 gaaagaagtg agacaacgca tcaataaatt tgtgcccatc cttgaacata aatagcaaaa
     4261 taaatggaac ggtaaataac aaaataaaaa attgcatcat aaatgaaatg atttttgaga
     4321 cacttgcaga taaactgact aatatgatat tgaacaaatt actaagagat aaatttgctg
     4381 atgctaattg ttcttccaaa ttgaaatttt taaactcttc ccgctgcgcc aattcattta
     4441 accatttgct tgtttcatct gcatacattg gcaagctctg ggtcaattga attgtctgtt
     4501 ccattaactg aggcactaca ttcatgacag ttaaaacaac taaaatgatt acgaccaaaa
     4561 acgtcaataa aaaaccgaac actctcggga tccgatgctt ttcgagaaaa acaacgattg
     4621 gatcgaacat ataatagata aaaccagcaa caagcaatgg taaaaaaact gccgaaagca
     4681 ttttcaatat tggactaaaa atattcggca tttgtaaaat aaagtacacg ccgatgatcg
     4741 tgagtactag ctccactgtc cagaaaaata attttgattg tttaaaacgt gattgcatac
     4801 atctcctcca aggtcctttg atacttctat tctatcattt aacaaagagt tacgcgctga
     4861 gattttcatt tgcttaaaaa aaacaaaaaa aataaacata ttttaatgag tagacgagta
     4921 cttaaacaaa aacacggttt ggagtagtga caaaaattat actgcagtcc ctttttccaa
     4981 ataaacggtg ttcaaaagct tgcatcttcg taataagccc agccaaacga aaaatataag
     5041 gaactatttt tatttggctg ggcttattac ttgatgtagg acatctattt tacaacctca
     5101 gatcatttat tgcgcaatac tgactaaatg caactttgtg atatttttgt gtacggacaa
     5161 ctcctcgatc acatcggcat aagtgagccc ttttggaaga tcaatcgtat agatattttt
     5221 gtacactcgg tagtcatcaa cgaattttac gtcaaaattc acatcttcga ttacgatatt
     5281 tttctcttca aaataatcgg taagaaaatc tttggtttct tttctatgga taaattggat
     5341 ttctaacttt ttagtcgtag gtacatggat cacgcgtttc actactgtga gtgcaatagc
     5401 aatcccgata aaactgatga tcgcaatgga atagtaaccc atgccgatgg cgatccctaa
     5461 tccagcaatt gcccataaag aagcggcagt cgtaagtccc gtcaccgatt gtttcgtgac
     5521 gatgatcgtt cccgcgccta agaaaccgac accactcacc acttgagcaa tcagccttgc
     5581 ttcatctgaa cgaatcgttc cagcaagctg tggatactct aaagcatctc ttaatgcacc
     5641 actagcgatc tcaacttgga tcaatgcaat gatacatgct cccatacaaa ctaaaatatg
     5701 cgtacgcata cctgctggac gatttttata ttgtcgttca aaaccgattg ctccgccaaa
     5761 tagcatagcg atccctaaac gtaaaataat ctctggaatt gtcaatacca tatcattcat
     5821 ctaaacaccc tccagtccaa tatatcatgt ctgaaaggag cagacaaaaa aagtgcaatt
     5881 tttacctctt acgaaaaaag gactgtgaca aaatttttgt catagtcctt tttatgaata
     5941 aacggtgttc aatcagctga ccctcggtat tactcaacaa acggcaaaat atggggagct
     6001 atttttcctt tgtcttctct aatactcagg tcaaaacgct gatttcacaa cctctttagc
     6061 ctactattga aaattacaac ttacttgctg cttcttcatc gatgatcaca acaacattgt
     6121 cgtgattttg caatacactt gctggcaaat gattcgttac aggtccttca atcatccctt
     6181 tgattgcatc agctttgctt tcgccaaatg ccatcaagac gatttcttta cttttcataa
     6241 ttgaaccgat tcccattgaa acagcttttg ttggtacatc ttctgcttta tcgaagaatc
     6301 gtttgtttgc attgattgtt gattctgtta agtcaactac tgaagtcgtt ccgtcaaatg
     6361 aagcaccagg ttcattgaaa ccgatatgtc cattttgccc gattcctaag atttggatat
     6421 cgattggatg tgcatcgatg atttgatcat actttttgca tgctgcttcc agatcttctg
     6481 cttttccatc tggtacaaac gtttctttga atggtttttt atcaaataat tggacatcca
     6541 tgaaatgacg gtagctttgt tcatctgaac cgcctagtcc aacgtactca tctaagttta
     6601 cagaagtcat ttcactgaaa tccaaatcgc ttgaagtcat ttctttatat aaagtaactg
     6661 gtgtgcttcc tgttgccaat cctaatactt ttgcaccttt gttcatacct tctttgatga
     6721 tttcaaatgc tttttttccg ccttcttcag cgttttttac acgaatgatt tccatagtga
     6781 ttgctccttt atattttggt atagcccaat tatagcattc tttttttctt tttacaataa
     6841 ttagtataga ccaaattgca tttttttcat tttcttgaca ataaatagga atccgtgtta
     6901 ggtttcataa gatggttcaa tcaaacaatc catgttgcat gaaaaccaag caagtgattc
     6961 actgtggacg ctttatgtct gcagtttttt tatgaaaaag gagttattat gagtatactg
     7021 acattaaaca acattaccca gcaatttggc gacaaaatcc tctatgaagg tgtcgatctg
     7081 caattaaatg ctggcgaaca tcttgggctg attggacaaa acggagccgg taaaagtaca
     7141 ttgattaaaa tcatcactgg tgagatctta ccagatgacg gacagatcca ttggcaaaaa
     7201 aatattcaca aaggttacct cgatcaatat gtggaagtcg atgaaacatt aacaatcgaa
     7261 gaatttctaa aaactgcgta cgcaaaagaa tacgaaaagg aagaaaaaat tgcttttctt
     7321 taccaaacat atagcgagac cttcgatgat tccttgctgg aaaaagctgg gaaactccaa
     7381 acggaattag accaaggagt gttttatcaa atcgacacct tagttgctga gatgagcagc
     7441 gggttgggga tcgatgtctt aggtttagat tcactgttga aaaatctaag tggtggacaa
     7501 cgttcaaagg tgattttagc aaaactactt ttagaaaacc cagatgttct gatcttagat
     7561 gaaccaacca atcacttaga tgatcagcat atccaatggc tgacgcagtt tcttcaggat
     7621 tttgacggtg cgtatatcgt gatttcccat gaccaagaat ttcttgatca gatcaccacc
     7681 catatcgcag acatcgagtt tggaaagatt actaagtata ctggccatct aaaacaagca
     7741 ttgaaacaaa aggaacaaaa tcgcgaaagc tatttgcgcc aatatcatgc tcaacaaaaa
     7801 caaatcgaaa agaccgaagc ttatattcga aaatacaaag ccggttcccg atcaacgatg
     7861 gcaaaaagcc gacaaaaaca attagataaa atcgaacggc tgacgcctcc agcttcagca
     7921 agtaaagctg tctttgattt tccattctcg cctattgtca cgacattagc agtggaaaca
     7981 actgatttag tcatcggata tgatcgaccg ctacttgcac ccatcaattt gaccatccgc
     8041 tttggtgaaa atattgccat tcgaggcttc aacgggattg ggaaatctac tttattaaaa
     8101 acactgatcg gtgagattga aaaaataagt ggcgatttcc acttcccaga taacacaaaa
     8161 atcaattact tttcacaaga tctgtactgg gaaaatccat tagaaacacc actacaatat
     8221 ttaagcagtc aatttccaaa agcgactatc aaagaattac gtcgccaact agctaaagca
     8281 gggttagtta atcaattagc aagtgaacct ctcactacat taagtggtgg ggaacaaact
     8341 aaggtgaaac ttgcacagtt gacaatgaac caaggtaatt tactgattct cgatgaaccg
     8401 acgaaccata tcgatcaaga aacgaaagaa agcttacaag acgggataca gaaatttcca
     8461 ggaacagtaa tcatcgtctc tcatgaacaa gaattttatc aagatcttgt gcaacgagtc
     8521 attgagattg aaccaaaata aacgtttgat tgaatagttg aaaaaaagga gctgtgacaa
     8581 aagttttgtc acaactcctt cttctgaata aatggtggcc aattagctga ccttcggtat
     8641 tagctcaaaa aacggcaaaa tatgaggagc tatttttatt tggctattct tattacttga
     8701 tgcagaacag cttttcacaa cctcttttcg tctatttttt attttcgaga tttctctttc
     8761 ctttaaaaac aactgtttga aagaagaacg cacatccttg aaaaacaagc ccgctaaccg
     8821 ttacacctaa ccagccatac tgctgccaca ttgtcgtccc tagcaatgaa ccagccgcac
     8881 ctccaataaa gtacataaac ataaatacag tattatttcg gttgcttgct gcttcaccta
     8941 gcccttgtac tcgtgtttga ttggcgactt gaccaaattg attcccgacg tctaataaaa
     9001 taatagctaa aagcaaaatg gccaaatggc ttccgccaac caataaagca ataaagctta
     9061 ttgtttgcat aacaagtccc atatacacga tttttctttc agaataagaa tctgccagtc
     9121 gtcccacgat aggtgcagca actgctccag acaaaccaaa aatcgctaat atccctgctt
     9181 cgaatgttcc ccaattgtag gcgggactgc tgatataaaa aatcaacgta gaccaaaaga
     9241 tcgaaaatgt tccaaacatg aaaaatccac tgactgccgc ttctcttaat agacgttgag
     9301 aagcaactaa atgcggcaaa cttttaagtg aatcaaggta tgaaacagct gtactacttt
     9361 gctggacaga acgcggcatt ttccagtaaa gcagaagtgt aagaattccg actgcacata
     9421 aagcaaataa atagatcatc cgccaagaaa aaagactggc aacaatacca gataccgtcc
     9481 gagaaagcaa aatccccacg agtaaaccgc taagcaatgt acccatgacc ttccctcttg
     9541 cttttggtcc cgctagtacc gcaccgtaag gaatgatgat ttgcggcacg atcgaaagta
     9601 atcctattaa aaaagaagcg catgcaaata acgtaaacga aggcgcaaaa aaagcggcca
     9661 acagagagac ggcagataac gctgccattc gaatgatcaa ttttggacga tccaccacgt
     9721 cccctaaagg aactaaaaac agtaacccta acgcatagcc taattgggtc aacatcgtca
     9781 atatcccgac tgcacttgtg ctaacagata aactagctgc tacttttgtt ccaatcggct
     9841 ggatgtaata catattggca acaacaacgc cacaagtgat tgataatagt aaaataagcg
     9901 aagttgttaa aggttttttt tcattactct tcattgattt cccccatttc ctatcttagt
     9961 ttacaactta tggaaaagat tggaaataga aaaatacaac tattcgccac acttattatt
    10021 tgtgcttttg ttttccttgt tttttcttgc gatctttata aatgaagaca actaacccta
    10081 ctagcacagt aacacttgca accaacgtga ctgcagtttt gttatatccg tgaaacagag
    10141 cttctccacc acacgcatac aacacagacg aagggatgac tccaatcacg atcgacaata
    10201 tgatttgctg tagaggcaat ttcatcgcat ctgcagcaaa attgaccata gaagatggaa
    10261 taaccggtat catatagcct aacattacac ctatttttgg atgtttcatt tgtcggattg
    10321 cttgcaccca atgattcgtt tcggttttct tatctaaaaa ttttaagcga tgcataagga
    10381 aaattgcagt gatgttacca agagcattac ccaaaatgtt gatgatcgca cctgtcagtg
    10441 gtccaaagct caaaccagca atgactccaa ctactgaagt aggaatacct ggaacagcac
    10501 acatcaccgc tgtcaatacg atcaagagta tcgatgcaaa agtcccatgc gaacgaatat
    10561 gtgcgagtag ctctgcctta ctcgctctcg gatttaaaaa taattggatg tctgtctgat
    10621 attctaaata caaccgatag cccactacca gtatcagaat gattcccaac gagatcaata
    10681 tcaatcggat tattttcttt ttcatatttc ctcctgaaaa aaatctatat tcattaaata
    10741 atagtttaac ataaaaacaa aaaagcaccg aaatccaaca taggatctca gtgcttttaa
    10801 atagtaaaaa ttatttaaca gttacagatg cgccaacttc ttcaagttta gctttcaatt
    10861 cttcagcttc ttctttagat acgccttctt taactggtgc aggagcgcca tcaactacag
    10921 ctttagcttc tttcaagcct aaaccagttg cttcacgaac tgctttgata actttaactt
    10981 tttggtctcc agctgcagtt aattctacag tgaattctgt ttgttcttca gcagcagcag
    11041 cgccaccagc agcagctaca gctacaggag cagcagcaga tacgccaaat tcttcttcaa
    11101 tagctttaac taggtcgtta agttctagga tagttgcttc ttttaactca gcaacgatgt
    11161 tttcaatgtt caatgccatt ataatttcct ccgtttaatt gttctttttt atttaatgct
    11221 ttatgcggca attaagctgc atctccatct tctttgtctg ccactgcttt gacagcgtaa
    11281 gccacgttgc ggactggcgc ttgtagtaca gatagcaaca tagatagaag tccttcgcga
    11341 tttggcaatt ttgccaatgc tgtgatttct tccaatgaag agactttacc ttcgatgata
    11401 ccgcctttga tttctaatgc ttttgcttct ttagcaaatt cgtccatgat tttcgctggt
    11461 gcgactacat cttcgttact gaatgcaaca gctgtaggtc cagtgaatac ttcgtccatg
    11521 ccttcaaggc cagctttttt agctgcacgt gaaagaatag aatttttgat aactttcatt
    11581 tctacgttag catcacgaag ttgtttacgt aaacgtgtta cttcatcaac tgttaatccg
    11641 cggtagtcaa cgactactac tgatgcagct gctgtaaatt tttcgcttac ttcatcaacg
    11701 attgctgctt tttttgcgat aattgcttca ctcatttttg agtttcacct cctgaatttt
    11761 gatggaagag ttttttgcac tgaaaaggca agtcactttt atagttactt tgctcaacca
    11821 gtattattca tcacctattc acttcgttca tagtgattct actcgctttg ctcgaaagga
    11881 aactcctttc gctttgctca accagtattg ttcatcatct cttcacttcg ttcatagtga
    11941 ttcacaataa aaaactctat gccaccgcag acatagaggg actattgaat atattcaata
    12001 aacgtcctcg gtaggaaatt aaggcttgat gccacctact gtcttcggta caagttaatt
    12061 attaacctca actagcttag catgctaaac tagttgagtc aagaattatt tttcagttgg
    12121 atcaatcaac attctaatat ttaattagaa agaagcttgg tcaacatgga taccaggtcc
    12181 aaatgttgtt gtgatcgtta catttttcat gtattgacct ttagctgctg atggtttagc
    12241 ttttagcaat acatcatgga ttgctgcaaa gttttcaact aatttttcat cgtcaaatga
    12301 aacttttccg attggtacat ggatatttcc agctttgtcc acacggtaag ttactttacc
    12361 agctttcact tcgtttactg cttttgttac gtccattgta actgtaccag ttttagggtt
    12421 tggcattaag ccttttggtc ctaatactcg tcctagacga cctacagtag ccatcatatc
    12481 aggtgttgca acaactacat caaagtcaaa ccatccgttt tggatttttt gaaccatgtc
    12541 gtcgtcacct acgaaatctg caccagctgc ttcagcttct tttgctttgt ctcctttagc
    12601 aaaaactaaa acagtttgtg ttttaccagt tccgtttggt aatacaacag caccacggat
    12661 ttgttggtct gcttttttag ggtctacgtt caaacggtat gcaacttcaa ctgttgcatc
    12721 aaattttgca atgtttgttt cttttgctaa agctactgct tcggcaacag gataaacttt
    12781 acttgaatca acttttttta atgcatcttg catttttttg ctctttttag ccattttggt
    12841 tcctccttga ttgtggttat aacggttgta cctcccacgt gttctatatc tttcaatata
    12901 gagccaactg agaagctgct gtcttagggt gtatagatta ttctacagtg atccccatgc
    12961 ttcgtgcagt tccttcaacc atgcgcattg ctgcttcaac gttagctgcg tttaggtctt
    13021 ccatttttaa ttcagcgatt tctcttactt gatcgctaga aactttcgca actttatttt
    13081 tgtttggttc gcctgaacct ttttcgattt tagctgcttt tttcaataga acagcagctg
    13141 gcggtgtttt tgtaacgaat gtgaatgaac ggtcttcgta tacagaaata acaaccggaa
    13201 tgatcaaacc tgcttgatca gctgtacgag cgttgaattc ttttgtgaat cccatgatat
    13261 tgatacccgc ttgacctagt gcaggaccta ctggtggagc tggagttgct ttacctgcag
    13321 gaatttgcaa tttaacgatt ttttctactt tctttgccac gagacatacc tccttaagtc
    13381 cgtgatgtgg ttaattggag atgcatattt ctcctcccac gttttatctg atctgaaaaa
    13441 atccagacaa ataatgtgca aaacaatgca cactggtaca gtatagcaaa tttatttgtt
    13501 ttttcaagat ttttttctgc tatttttaca ttttgcttga ataaacgcta aatttattct
    13561 aaaaaaacaa caatttttcg ttctcatttc ttcttttatg ttacacttgc gctagcatgg
    13621 ttcaacagat gaaagcaaac aataacacgg ttttttagac ccttgtacaa agattcctac
    13681 ataacaaaat gaggtggaga aaatgaaaga tttaaggtat agaagtgtct ttgatattat
    13741 tggacctgtg atgattggtc caagtagctc tcacactgcc ggagctgcta gaatcgggaa
    13801 aatcatacgt agtatatttg gcgaacagcc tgattctgta gatatttatc tatatgaatc
    13861 atttgcaaaa acctatcggg ggcacggaac agatattgca ttagttggcg gcctgttagg
    13921 gatggaaccc gatgatgaac gtttagctga ttctttgaaa ttggcatatg aagccggtat
    13981 ggaagtcttg tttgttccta agagtgaaaa agccgaacat ccaaactctg taaagatgct
    14041 tctaaaaaaa ggaacacgaa agctttcagt cactggcatt tcgatcggcg gtggcaatat
    14101 ccagatttct gaattgaatg gattcaaaat ttctttaagc atgggtactc ctaccttgat
    14161 cgttgtccac caagatgttc caggtatgat tgctaaagtg actagtatac tttctgagac
    14221 caatacgaat atcggcacta tgacagtgac acgggaatca aaaggtgaaa atgccattat
    14281 gataattgaa gtagatgatc ctcaagtaga agaaacggta aaaagactca aacagctgcc
    14341 aaatatcgac agcgtaaatt atttcgatta aggagctaaa aaaatgtttt tatcgattga
    14401 agaattagta gatcaagcaa aagcttatcc aagtgtagca gaattgatga ttgcagttga
    14461 aatggaaatg agtggccgtt cccgtagtca catcatcgag atcatggaaa aaaacctctc
    14521 agtaatggaa caatcaatca acgagggaaa agaaggtgtt acctccgtca caggaattac
    14581 cggtggtgat gcaaaaaaat tagatgatta tttaaatgag gcagactttt taagtggaga
    14641 aacaatctta aaagctgtac gcaatgctgt agctgttaat gaagtcaatg caaaaatggg
    14701 gttgatttgt gctacaccga ctgccggaag cgcgggtgtt gttcctggtg tgctgatggc
    14761 tgtccgagat cggcttgatt tgacacatga acagcaattg gaattcttat ttaccgctgg
    14821 cgctttcggg ttagtcatcg caaacaacgc ttctatcagt ggtgcagaag ggggatgtca
    14881 agcagaagtt ggttctgcaa gtgcaatggc agcagcagct ttggtttgtg caaaaaatgg
    14941 gactcctcat caagcagcac aagcagtagc cattacattg aaaaatatga tgggactgat
    15001 ttgcgatccc gttgcaggct tagttgaagt cccgtgtgtc aaacgtaatg ctttaggctc
    15061 ttctcaagct tttatctctg ctgatatggc attagcgggt atcgaaagtg tcattccgcc
    15121 ggatgaagtc gttgctgcaa tgtaccaagt cggacgtcaa atgccgtcga ttttcaaaga
    15181 aacagcagaa ggtggcttag ctgttacgcc aactgctaaa cgacttgcgg aagagatttt
    15241 aaataagagg gcgtgataaa gctccttaat ttcgagtatt aagagattag ttatcttcat
    15301 aacaaaaaac gaagagagga ctaattgccc ttctcttcgt tttttattaa tccatcaatt
    15361 tcgtattttt tacttcatcg atcgtttttg ttcctgcaag ttgcatcgtg atcgacaatt
    15421 ctttgttcag gtgttcaaaa acagatgtta ctccttctgc cccacctaga ttcaacccat
    15481 aaatcactgg tcggccaatc gcaactaagt cagcaccact tgccagagct ttgaagacat
    15541 gctctcctcg acggacacca gagtcgaaaa tcactggtac acgtttgttg actacttcgg
    15601 caatttttgg caatacttca aaggacgcag gacctccatc tagttgacgt ccaccatgat
    15661 tggataccca gataccatct gcccctgcag agatagcgat tactgcatct tctggcgatt
    15721 gtattccttt tacgataact ggaagatggg tgatttcttt gattgttttg atgtcatctg
    15781 gtgtcaagcc ttgttttgca gctgcataaa tttctgcgat tccttttcct tcgccattac
    15841 cacttgcact ttgttcgcta tacgcagcta agttaggcat tggtaatgga aattggaatt
    15901 gattgatgac gtcttcttca cggtaaccac cgagcgttga atctgccgtt aaaatgatag
    15961 ctttagcccc tgcttctact gctttgttca aaatgaactc attgaatctg tcgtctttac
    16021 tcatatataa ttggaaaaac tgcggtgcgc ctggcgctgc atcggctgcg tctttgattg
    16081 tcgtatttgc ataagtgctg atggaaaaga ttgatccagc agcagctact cctttagctg
    16141 tatcagcttc ccctttttca tgtgccaatc cttgggccgc tgatggcgct tggataatcg
    16201 gtgtatctaa ttcaattccg aaaacagatg tatgcaaatc ggctgagtcg attccgcgta
    16261 atatccgtgg catgattttc tttgtattaa aagaagtcgt attttctttc atcgtccact
    16321 catcttcaga accaccgcga atatagccaa aagctcctgc ttccatgcgc tcttttacac
    16381 gaccttctag ttaggcaatg tttacaatat cgattggatg ttcttcatta cttgtatgat
    16441 aagtcatagt agatccttct ttcatcttct tgattacata taaatcatac taaacttaca
    16501 aaaagtaagc aaacatttac tctcagatac ttttatcgct attctttcca aataaatcga
    16561 cttacaagct gatctacttc ttggttttca tgaagcatac tatgagaggc attttcacct
    16621 gtcactactt tttcctctat ttcatttccg cgttgcttca acaacgcata gaccgctaat
    16681 gcactgttta acggtactgt tccatcactc aagtctgttt catctagttg accagcaatc
    16741 aagaaaaatc gagtggttat aggaatatta ccgatcattt gctggtaatc ttgatagcgg
    16801 ttagaaacga cagcaggccc ctttgctaac tcatctgtca gagactgcgc actatcatcg
    16861 acaaaatcat taaaaggcgc gccaattgct gttaatgtct gaacttgtgg caatgacgta
    16921 tcctgtccat acgttcctaa atagcgtagt gcactaactc ctcccatgga atgccccact
    16981 atgttgactt tatctatgcc gtagttttct ttcagataca aaagacaagt tttgatccat
    17041 tctgcttgat tccactcgtt atttttatta tcttcaaaca atacttggac catcggattg
    17101 tctgcttttc cagacaaatc tcctgttgct tgtatttgtc catctgttcc tactgttaaa
    17161 accaattctt ttactgcttg ctgatttttt tccaaacgcc gcaacatccc tttaaaggaa
    17221 ttttctgtcc cgctgtagcc gtggaaaaaa agtgttggaa tagtggtttt ttctatcact
    17281 ttactggatt ctgccgtttt ttctttatca gaaacttcag tagtatttat cttcgtcgtc
    17341 tggcaaccac ttaacgcaaa caaactgata aggactaaag ctgctgcagt tgcacgtttg
    17401 cataaatttt tgattggttc tgtcatacac ctcttccttt ccatatagaa aaaagaaaga
    17461 cttgaaaggc caatcatatg atcgacattt caagtctctt tattaatcga ttttatctac
    17521 ttgatcgaaa tcaagttctg tactggtttc acgtccaaac atatcgatgt tgactttcaa
    17581 tttttctttt tctccgtcaa cttctgttac ttgtccttct aatccagcaa atgcgccttc
    17641 gatgattttc acgacttcgc caacttctac ttctagatca ttttgtcgca ggctcatacc
    17701 gatagaacgc aagatatgat tgacttcttc tggaagtaat ggtgctggtt tgcttccggc
    17761 tccgtgtgaa ccaacgaatc ctgtcacacc aggtgtgttg cggacaacat accatgaagc
    17821 gtctgtcatg atcatttcaa caagtacgta tcctgggaat gttttatgaa cgatttcttt
    17881 ttctttccca tttttgactt cagtttcttt ttcttcagga acaacgacac ggaaaataaa
    17941 gtcggccatt ttcatacttt gcgcacgaga ttcgatattt gcttttactt tgttttcata
    18001 tcctgaatat gtatgcaaga cataccagtt tttttcaaaa gattccattg aaggctcctc
    18061 atttcattgt aaaataaaaa aacctcagta cacccgaagt ttttctttcg cttcattata
    18121 gcactaaaag tacgttgtgg ctagcgagtt attttaaaat ccagccaaat aagctttgga
    18181 tggctgtatc cattacgtaa aacatcaatc cgaaaataat agaagtttcg atgactacta
    18241 acgtatcttt acggagttgt tttctgcttg gccaagttac ttgtttgatt tcatctttta
    18301 cgctttttag aaatttcatg agacaacctc ccttgctact gtttatttcg tttctttatg
    18361 taatgtatat tgattacaat atttacaaaa cttattgatt tccaaacgtt cgccgctttt
    18421 tccttcactg actgattttg tgtagttgcg agacccacaa acggaacaag cgagtgctgc
    18481 ttttttcttt gccatttact tctgccccct tgctgagaat cagacttata cttcataaca
    18541 gtatcacgta actttttact tgtcaatttg tgaaataaac tatctattgt tttggataaa
    18601 attatgatat gattaatttg aaattaagct aaaatgagac aattggaggt atagcgatgc
    18661 tattaaaaac acttgtatat aagaaaaaag atctgacaac cgttacagag actagtacat
    18721 tagaagaagc attaaaaatt ttagaaactt ccggataccg ttgtgtgccg atcttagatt
    18781 cttcaggtaa tattttccgt ggaaacattt acaaaatgca tatctatcgt cataaagcaa
    18841 atggcggtga tatgacgctt cctgtcactc atcttttgaa aaacgctaca aagttcattt
    18901 acctggatac ttctttcttc aaagtattct ttacgatcaa agagcttcct tatattgctg
    18961 tccttgacag tgaaaatcat ttttatggta ttttgacaca tagtacgttg cttaatatgc
    19021 tctctcaatc ttggagcgta gaacaaggaa gctatgtact gacaattgct tccacaggaa
    19081 agcaaggaga tttggctgct attgcaaaaa tcatttcaaa acacagcagc attgccagct
    19141 gtattactct tgatatcgaa aaagacgaat acattcgacg tacattgatc acgctaccgg
    19201 ctaacactga agcgaaagta tgtgaaacga tcgtcaacca cttagagaaa aagaattata
    19261 aagtggtaga aattgaaaat ttgcaaaacc aagaagcata atcaaaaagc gaaaaaagaa
    19321 aacagctaat ctgctttctt ttttcgcttt ttataattag acacgctttt caaaatcaag
    19381 cgcgcgatct aaataataca tcaatggtgc tgaaacagcc atgacgataa actcacttaa
    19441 tgcaagcgtg ccataggttg tccaaaatgc ttggccacct gttggaacta acatataagc
    19501 aatcagtccc atgctgatgg taaagaagat cgtgttcaat actaaacgca tctttacatt
    19561 tttcactttg ttttgcaata aagctgtcag gcctaaagaa agcaatgttt ggccgccacc
    19621 aaaaaccaca tccatgatac caaaaccaaa gaaggcatta tagacgatga ccccgcctaa
    19681 aactccccaa agcaatttac ggttaaagac aactaaatga ttcaaacttt ctgaaatacg
    19741 aaactggatc ggccctgagg atacaggcgc aactaaaata gttaacgcta gataaagagc
    19801 catgatgatg ccatttacaa cgattacgga taaccgttgt ctgcttttcg ttttttgcat
    19861 tttctctcct cttttctcct agttttttta acgcgggatg gttgatgaac cgcggttatg
    19921 tgaaacaaac aataattata acagactttt catttatttc aatccgtctt atctgctttt
    19981 aaaaaagcaa aagcttaaac aatgtaaacg tttgctaaaa atcgtgggat atggtataaa
    20041 gtaagtgtaa ggggtcataa gaattcccag taaagcagac gaagtaggat ctgcaagaac
    20101 agaattgttg tgaaacttaa ataaataaaa aaggatgtgg gaatttgagc aacctcttac
    20161 tagagaacgg tctagcaagc agctgaagat ctaagtggac atgccgaacc ttggaacttc
    20221 tccttacgta agtaatggat catgcgcttg ctaggctttt ttgtgttttt cggtcttcgg
    20281 acctttttca ttttcctaga cttctgctat aataaccaag aattaatttt gtaacagatt
    20341 tttttgaaaa aacaagtttc tttcattcaa aaaatagaaa aagcttgcta attattggtt
    20401 aaccagcaaa caggagtatt aaaatatctt gttttattga atagcaggag gaaatcatga
    20461 aattccatta tcatatattg atcaatccat cggcaggcag cggaaatggc tataaagtag
    20521 cagaaagaat tttaccggta ttaaaaaata agcatattga ctatacactt cattatagtg
    20581 aatacaaagg acacgatgct gagattgctg aaactttggc aaaagaaaca ttgattcctt
    20641 ggatagaaga agaacagaaa acaatcgata ctttccccct tttgatcatc gtaggaggtg
    20701 acggcacctt acatcaggta cttgatactt tctatcagat ggaagtagaa ttcccagttg
    20761 cttatattcc agcaggttct ggagatgatt ttgctcgagg ggcaggcctt ccaaaaaatc
    20821 ccaaaaaagg acttcagctg attttagcag ctcaatcacc agaaaaagtc cacataatgg
    20881 cttatgaaga aaaaataagt gaaaaaaaag gaatagctgt taataatttc ggtataggat
    20941 tagatgcagc tatcgtccat gcgacaaatc attcttccac aaaaaagcga ttgaacaaat
    21001 acaacttagg atcattttcg tatttattct ctattctccg tgctttattc actcaaaaag
    21061 gatttccaat tctggtagat gcaggaggaa aacaattaag tttttcaaac gcatttttat
    21121 gtacagcaac gaagcatcct ttttttggcg gaggaatcgc gattgttcca actgctgatg
    21181 cgtctaaacc agtcattgat tttgtcgtgg tcgaacgaat caatttcttt aaaatcttgt
    21241 ggctcattct tttacttata cagaaaaaac aattgaaatc gaaatatttc catcactaca
    21301 caacaaacag attacgtatc gtttcaacaa caccgcaaca tggtcaagaa gatggagaag
    21361 aaatggaaaa acgacctttt gatatcacaa taaccacaac taaacagttg ttttggtgtt
    21421 aataaaagag gttttgtcag aagctacatc tggaacaccg tttattcgga aaaaaggact
    21481 gtgacacgac ttttgtcaca gtcctttttt tactatgcag ttaattcttt gcccaattgg
    21541 gttgccatcg atagtttctt tctttaccca aaataaattg gatcattgaa aacaccgcag
    21601 caaaagcaac taagacacaa ccattgaacc aaaaagattc tgcaaatgtt tgattgtaac
    21661 tgagtgtttc actgatcaag ttattatttt gaacaaacca atatgttggt gtgaatgccg
    21721 ctattttgct aaccatttct ggcaagtatt ctgctggtac aaacacccca ccgataaagc
    21781 aactgcctgt gataaaaata tctttgatcc caccgctggt ttctgagttt tttaccaatg
    21841 aagtgataca agccgaaaag ctaatgatcg gcaataaaaa caagaaagta tttaaaatat
    21901 gataccatac cgttatatcc cacgaggtgt ggatcactaa cagaaccatt agaagtaaga
    21961 agccactgaa caacagcagc gagtaactaa tcaaactaaa ggttactttg cgattcaagt
    22021 ttcgtttggt aatgggcgca ctctggtttc gtttactgat ctctttgcga ttaaatgcta
    22081 gattgatgac gctgaaaccg ctaaaaatgg atagaaacaa cccataagac aagaaattaa
    22141 aggttgtgcc cgtcaacagc tttttcatcc gctgactata tcccgcactt agctcaactt
    22201 ctccagtaac cgagagtgcc atttgtgtct gttccatagc tgcatcgatt gttttatctt
    22261 cttcttgaaa cagaatcagt gtttgcaaaa aggtgttcac ttgctgtgta acaactgttt
    22321 tagaaaaggc atctgggcga ctctgtatct tgatcgtcgg catctttcct acttcgactt
    22381 gttcggtaaa gtctgcaggc agatgtaaga taatgtttac acgattaaaa taaagagcat
    22441 catcgatttc tttttgtgag gttccatcca atgtaacgac tgtttccttg tcattcaaat
    22501 atttacgaag gccatcgact acaggcgact cttcttcact aatcaaggcg attttcgcat
    22561 cttgcagaga ggtattttca acatcaatat tttgcgcaaa aaagaatgtg gcaacaacag
    22621 tgataattac acctatcagt aaattcgatt tatttgcttt aagcaatgtc caaatcgttt
    22681 tataaagcgt cataacgcac ccctctttcc atccaagcat ttcctaacgc aaaaaggaaa
    22741 ataaatccga tcaaccatag aagattggta taaaaactag ccaaatcagt gtagaaataa
    22801 agtttataca aactttcgct gatcaagttg acgagattta attgaccaac aatcggaata
    22861 tgaagatcaa tccaatactt cacttgttcc gaccccatca taccagcaaa aaagctcatc
    22921 gcaatcgtca ctgaaatcaa gatgctatcc ttttgcacca gattcatttt tggtatcaaa
    22981 ttaccaacca atgtacctaa caaaatcgca cacaacgacc ccagagcggt aacaatcatc
    23041 aaccattgcc aacgttcacc aaagtctact tgatagataa aacgaaagac agctaacata
    23101 atataaatct ctgtcaaaac aatcgtaaag cttgctgcca aattagcgat tgaaactaac
    23161 gattgctttc taggaatcaa ggacaagcga ataccatttg acgattgatt tgcttgctga
    23221 tcctgtacat tgcggacccc ccacatcgtc ccgtagagaa tcgccattcc aatcagagtg
    23281 aagaaataaa aacttttgaa actcatatta cgacctgcat gaacttcttg aaaagatact
    23341 tcttgttcaa aaacagtcat gatgggcgcg agatcaacgt tctccacttc tgccacttta
    23401 ctcgaaatta taccaatacg ctgtaaaaat tgatccatga cgtttttcat caacgtctga
    23461 ctcattcctg cttgacccac atggagcgaa acagatgaac catcaatgag ataaaaacca
    23521 gccaaatcat ctgcatttag cttttcattc gcttctactt ccgatagttc ctgtacttca
    23581 aataatggtt tatcattgac ttcgacttgt tctaagactg tggtaaaatt ttccgaaagc
    23641 gctgtttgat ccgctttgat tacaattcca gtattaatcg tttcgagatt gccaccttga
    23701 tcaatctcgc caaaggccgc cataaacatc aatcctaaaa gaattggaaa ggccaatgtc
    23761 caaaacaaca tggatttatt tcgcagcagt atttttgtgc gatatttata aaaatgccaa
    23821 aacattattt cccctcctct agtctctcaa atccttgcca gtaatctcta aaaagacatc
    23881 attcaaggac gcttctttgg ttgctatttt tccgtaactg atcttttctt cttgtaagat
    23941 tgtaagaatc ggcaacaata ctgtatttag ttcagtagtt gttacttgga gctgaccttt
    24001 tttataagcg attccatcag aagctattgt tcgctgacaa cacgttttct gggagtaatg
    24061 ggatgtcgat cgtcaaacga tccgccgtcc gtaccatatc ttttagagat tctttggttc
    24121 ctttagcaat cacctgtcct tgatccatga tcacgatctg gtcgcaaaga atttctactt
    24181 cttccatgta atgtgttgta tagacaatgg ttgctccttg tcgattcagc tccttgatac
    24241 tttctaaaat tttattccga ctttgggggt ctacagctac tgtcggctca tctaaaaaaa
    24301 ttaaccgcgg cttgtgggca atcccacatg cgatgttcaa ccgacgtttc aagccaccac
    24361 taagttctcc tggacgaaac gaaacatatt tttctaatcc cactaattga atcacctctt
    24421 caatcaaggc tttccttttc tctttttcgg aaacatataa accgcaaaaa taatcgatat
    24481 tttcttgcac acttagttcg tcaaagaccg caacgtcttg aggaacgacc ccaatcattg
    24541 ccttgacagc ataattttca ggagtcattg ttgtcccgaa caatttaatg gttcctttgt
    24601 cataatttaa caaagaaagc atacagttga tggcagtcga tttcccactg ccgtttggtc
    24661 ctaacagacc taagacttct ccttcttgta cttgcagatc aaaatgattc aatgctacaa
    24721 aatctccata gcgtttgact aatttatcaa tttcgataat catcatgaaa aactccttcc
    24781 aaatttcttg ttgatctgac tataataaag tgcaagaaaa gaaactagtg acgattggct
    24841 aaaccacaca tgacatttgt cacaatgaag gagtctgcta tgcataaatc tcggctactc
    24901 aacgaaacta gctttctact ggttatctca ttgcttttgc tggtaagtca aggattcgtt
    24961 gctatgcaag ttatctattt attgatcgcc gttttattca ctggtgtcag ttatttactg
    25021 cctacaaagc caaattggat tttccaagtt attctatttc ttttatgtgc ctttttacct
    25081 gatcttgtcc tgtttctgcc agcgacgatg cgagtgatcg cccctgaaga aagccacctt
    25141 gattacttgt ttttgttagg ttttttgttg atttcgacgg ttggtaatca tccccccatc
    25201 aaggtcgcct ttgtcgtaat tttgctgttt ttttcctttt acttacgaaa aaaagatcaa
    25261 cgaacgaccg atcttgagaa aaagtacatt gtctccaaag atactcattg ggaaaaacaa
    25321 caagagttat cggaacaaaa taaagcattg gccctgtcac aagaaacaca attagccttg
    25381 caaattgtgg aagaacggaa ccgaattgcc cgagacatcc atgacaatgt cggtcatctg
    25441 ctgtcaagtg cgattttaca aattggtgcg cttgaaatga tcaatcaaga accaaaacta
    25501 aaagaaccat tagaaaattt aagtgccacc gttcacactg ggatggatcg gattcgtcaa
    25561 agtgtccatg acctccatga gacatcgatc tcttttcaac actccttaga gtttctgatc
    25621 gctgattttc cttcttctgt tacgattgaa ggaaacttgt ttgaaggatt gaatgaaacg
    25681 cagcaaaact gcttcttaat ggtcatcaaa gaagccttaa ccaacagtat caaacatagt
    25741 catgccaaaa ccgttgtttt acattttcga acgttgccgg ccttttatcg cttgcaaatc
    25801 acgaatgatg gcaccagccc acaaaaaaat tctgctcccg gtattggttt gaaaacgatg
    25861 cgtcaacgtg ttcaagaaca aaagggacaa gtccatattt tccaagaaaa ccagcaattt
    25921 cagttaacga tcattttacc aagggaggga caccaatgat aacagtagtc attattgaga
    25981 tgatccattt gtgacgaaat cgttacaaac cattttagaa agcacgaaag agatccgtgt
    26041 gttaggcatt ggtcattgtg ccaaggatgc gctagtctta tacgaaaccc atcgtcccga
    26101 tgttttgctg acggacatcc gcatgcctaa acaaacgggc atcgatgctg ccaaagaaat
    26161 tttggctaaa tttcccaaag ccatcctttt gcttttgacg acattcaaag atgaagaata
    26221 catccgcgaa gcattttcca tcggtgtcaa agggtattta atcaaacaga acttgcaagc
    26281 gattattcct tctgtcaaat ccgcttataa tggacaggcc gtatttggta atgaaatcat
    26341 cgagactttc actcaaatga tgaagaataa accgacgatt gatagtcatg cttatgcttc
    26401 ttttagtgaa cgtgagcttg caatcatcaa agaagtagct gccgggaaaa acaataaaga
    26461 aattgcagat gccttgtact tgagtgacgg cactgttcgc aattacatca gccaattact
    26521 agaaaagcta gatttacggg atcgcacgca attggctatc tatttttatc agcatttgca
    26581 ataagtctta tctaacacaa aaaaacctga atgatgctcc ttcgcatcat tcaggttttt
    26641 ttcacatctt ctattcaatt gtcccgcaaa ccatttcctc acgattgaaa gaatcaagga
    26701 ttcgcagggc tgccaggatt cgttggattg ttcggcgtgt taggcggaaa aataggcgcc
    26761 ttgtctaacg ttttttctag ttttgattct tcacgatcgt ctcgttctac atcaggactt
    26821 tcattcaatt tgaattcttt ttccatctca tctaactttt taaagtcatt gccagattct
    26881 tttgctaatt tcgctaattt atcttcggtt tctttagcca taaagtaacg cccccctttt
    26941 gatttcagct tagcatatca cactgaaaaa acaaaagaaa acgctaacca acgatcattt
    27001 ttagctattt ctcatcttta aaatcaagca ttttttgccg gtataaaatc gtagggagaa
    27061 ctgttttcca taccaatcat cggatctact aactggttat cgatcacttc tgctgtaaga
    27121 acgaaatctt tagaaacagc aggttctacc tcttctttct tttcttctcc aacaaatgaa
    27181 gaaaacagat ctggttccac tgtttccatt acttggtttt tcgtttcatt cgtcccactg
    27241 tcatcttcta caggtagctt ttcttttaca acccgtggct cttttgtata gttcccatct
    27301 tcaaatggat catttccgac caaagaatct tcataagctg ctagtttggt ccggagagtg
    27361 atattcattt tatcattatc catgatcctt gatttcaacg tagtcatccg gcttgctgat
    27421 tcattcgcta cacaagtgac ataggcttgc tcttccccta acaaaatcaa cagttcctta
    27481 ctgcgagtca ctgccgtgta gagcaaatta cgctgaagca tccgctgata ttgatggacc
    27541 atgggcaaaa taaccatctt gaattcactg ccttgtgatt tatggattga gcaacaataa
    27601 gaaagagtga ttttattcca ttcgtttctt ttataagtga cctcatttgc atcaaactgg
    27661 ataactaatt catccacttt atcttcagaa tcttttgcca agatgatacc cgtgatttct
    27721 cccatatctc cattgaatac attcaattcg ggagtattga ctaattggag gactttgtct
    27781 cctatccgat aaacggtgtc gttccaatgt acttcctttt ttccaggttt ccctggattg
    27841 aatatctcct gcatcatttt atttaacgca tcgatccctg caggtccccg atacattggt
    27901 gctaatactt ggatatcttg tgcacggaat cccttatttt tcgctttttc cacaatttta
    27961 gagatcaagg gttcaatttg ataagcatca cttcggaaat aggaacgatc tttttgattc
    28021 tttgtgaaat cttttggcag acgtccttct ttgatctcat gggcaagagg aataatacta
    28081 gaaccatcac cttgccgata aatctgattg agttccattt tgggaatttg ttcgatttcc
    28141 aaaagatcat ggagtacttg accaggaccg acagagggaa gctgatcttt gtctcctact
    28201 aagataactt gcatattcgt cggaatcgct ttgaataatg tgttagccag ccatgtatcg
    28261 accatagaca tctcatcaac aatcaaaagt ccgccttcta attctttcgt actgactgaa
    28321 ggtgtttttt cccgtccatt cagccccaga agacgatgga tcgtactggc aggcagacct
    28381 gtcgtttcgt tcattcgttt agctgctcgg ccagttggtg ctgcaagtag aataggaaaa
    28441 atttcttggg tatattttga aggatccaat tccagtccgt tcaactctgc aaaaagattg
    28501 acgattccgt tgatcacagt agttttcccc gtacctggtc ctcctgttaa aataaataga
    28561 ggggaacgga ttgcttcagc gatagcttgt tcttgcgaag gtccatactg tatgccgaat
    28621 attttttcga tacgccgaat attcttctgc acttcttttt caggataatg gatttctttt
    28681 ttacgactga gaaggcgctg gatcgaatta ccgatccccc attcagaaaa gtacaaactg
    28741 ttttcgtaaa gattagtctc ttcttgttgt atctttcctt cttcaacaag ttggataatt
    28801 acagtagcaa cctgatcagg ctggatttca attgggcgac ttgtttctaa aatacggatc
    28861 gtttgctgaa gcaatgtctc cgccggtacg tatgtatctc ctgtttgaac tgcatgctga
    28921 aaaatctgat gcaagatagc tgctcgaatt cttctcggtg aatctgctgc aataccgatt
    28981 tgttcggcta gattatctgc acgtttgaac ccgattcctt ctatgtcttc gactagctgg
    29041 tacggatttt cttcgatgat atccaaagac tcgttcttat aggcctggta gatactgaaa
    29101 gctaactgac taccgaaccc gtaacgattc agtcccatga tcacttgatc catcccgtgg
    29161 tttagccgaa tcgtctcagc aagcatttct ctttttggct tcgtcaaacc ggagatttgt
    29221 tttaataccg aaggatcagc aatgattttt tcgatagcat cttctcccag caattctaca
    29281 attttttctg ctgttttctt cccgattcct gggaattttt cgctggaaag atacgctacg
    29341 atcccatttg cagttgttgg cttttcttgc tcatatcttt cgagtttaag ctgccttccg
    29401 tatttaggat gatccactaa ttctccataa aaacgataag tctcattttc ttgaatatcg
    29461 ccgaaacttc cggtgactac aatctctttt tcacggtaat cagctgtggt ttcactgatg
    29521 tgtaccaaaa gcactttgta aaaattacta ggattttgaa aaaaaatcgc ggcaactgta
    29581 ccaacgaaat aaggcatttc ttctggctgc atcggaagcc gcctccttct tctatagtaa
    29641 aggttctggt gctgtttcag ttcctaaaaa atctgtttgg ttccatactt tcagttcata
    29701 tggcaataat ctgcaaggct cttcagtctc taaaatcgtc aaactactat tgaacaatcc
    29761 acccatttca cgcaactcac ttagctcttt tcctgtcatc cattgaatgg ctgcagttaa
    29821 agaagctcca tgtcctacga ataaaagtgg gccatcttta tgttgggcaa ccgcatctgc
    29881 tacaacagtt tcgatacgtg tgattgccga ttcaatcgtt tctccgtgaa aaggagaagg
    29941 atcatacaga tctaaatgat tacgcaaatt cggcagatta tccggatatt tctggtacat
    30001 ttcatcgatc gactgacctt ctaaagtacc cagccctagt tctcttagct tatctgtata
    30061 aaaaatctct accggacgag caagctcttt gttgatacct tctgctgtat cacgtgctcg
    30121 ctttgctgta ctggaataaa ttgctttaaa aggaacatat tttacctttt tcccaaatgc
    30181 tttgatttct tcgtaacttt gtggcaaaag cggagaatcg ccattgctcc cttgaaaacg
    30241 tctttcctga ttccattgtg tcttcccatg tcgggtaaaa tatagttcca tacgcagcaa
    30301 actcctgtct ttcttttatt ttactctata ataaactgtc taatccagct tgtcattttc
    30361 atagtgatgc gtcaattcta aattagggaa tccttgctgg cgaagtgctt catacactat
    30421 cattgcagct gtattcgata aattcaatga gcggacatgc tcatcattca tagggatgcg
    30481 taaacacttt tcttcgtttt cacgcataaa tgtttctggc aaaccggttg tttcttttcc
    30541 aaataaaaag aagtgctctt ctccatcagc aaaattttcg tcactatata ctttatgggc
    30601 aaattttgag atcaaatgta gtttacgatt acctaaaaat gttaaaaaag cttctaaatt
    30661 actgtggtaa gtaatattta catcattcca ataatccaac cctgcccgtt ttagatgttt
    30721 gtcatcagtt gaaaaaccta gtggctcgat taaatgaagc ggagtattcg tggctgcaca
    30781 cgtacgggca atatttccag tgtttgcagg gattaatggt tcaaataaaa cgatatgatt
    30841 catcaggatc attcctctca tatttatgtt cgtttctatt ttctgacaat ttgatttttg
    30901 cataaaaaaa aacgtatcaa ctcggtgtca gtcactgcga gtcgttacgt tagtgaagct
    30961 cttctcacta acttttatca gaacaggaac ctgataaaaa ccaatgaaaa aacaacttgt
    31021 tgctaatata acaccctcaa cttttttttg caacctctac tcattaaaaa ctgctagaaa
    31081 aacgttttct cttagttcct tttaataatt ctatgtcaat tgtaatcttc ttcaaaggat
    31141 tcttatataa gtctgctttg attcgctctt ctacctgcca ttctaaaggt gacatggcca
    31201 aaagatcttt ttgattttcc tctggtatct caaattcata ggttacacgc tgacgttcag
    31261 ttaatttcat tgcttcagca aacttggcta ctacacgttc attggaataa ctctgttttt
    31321 caggttgatt cggataaaaa gcctgacgta gttcctttaa ataattttct tgtggcacca
    31381 ccttcaacac ttctccatct tctgagagta cacgttggaa ttcttggtaa tgagaaggcg
    31441 aaaagatatt taaaaccgcg ttgaatgttt gatcagcaaa cggcaaattg gtcaaatcag
    31501 ctacacacca aaatgcttcg gcagattgtt ctgtagctgc gtagactcct tcttttgaaa
    31561 tatcaaatcc aacagcattt tgaatctttt tcaattttga aagttcattc aagaagctac
    31621 cttctccgca gccaacatct aatagcgaga cttcttcagg taaatgctgc gcaatagtct
    31681 caataacggg ctgatacatg ccagattgga tcattctacg ccttggaacg aacattcctt
    31741 gatcgtattc tgttttaacc gcatgggata aaaaataaag tgttcctttt ttggaaaggt
    31801 caaaacggtg attatttttg catatcaagc cattatcaga cttttttact tcttcttggc
    31861 aaaccggaca gcggaacagc tgatgatttt ctgccaaaaa ctggcgtcct cggtcaattt
    31921 tttttaacat agttccccct catttctgtt ttatcttacc gttagatgct cactttggca
    31981 actgctaaaa atatgataca atgcctagaa gaaagtgaaa gataaaggag aagattagat
    32041 gatcaaagaa ttttgcgctg aaaactacac atctattcct ctagcaattg ctagaggagc
    32101 cgatcggatc gaattgtgtg acaacttagc ggtcggtggc acaaccccga gtactggtgt
    32161 gatcgaagaa acgttaagtt atgctgggga aaaagatatc cccgtaatga cgattattcg
    32221 tcctagaggg ggcgacttcg tctataacga tatcgaattg aaaatcatgc atactgattt
    32281 gattgaagca aagaaattag ggacggatgg cgtagtattt ggttgtctta ctccttcagg
    32341 ctggctagac gaagaggcct tagaactatt gatcgaaaca gcagaagggt tgcagatcac
    32401 cttccatatg gcttttgaca gtattcctga agatcggcaa ttcgaagcaa tcgattggct
    32461 ggtagaacat ggtgttgacc gtatcttgac tcatggcggt cctgctgaca aaactattgt
    32521 agaaaatttc ccacggctta aagaattgat tgcctataca gatggccgct taatcatttt
    32581 gcctggtggt ggtgtcactg cagagaatgc agcacatgta gctaaagaat tgaacgtaag
    32641 cgaagtacac ggaaccaaaa tcgtggatct tcagttataa tcttataaac aaaaaaacat
    32701 ttgcaacagg cataatgctt attgcaaatg tttttgtttt tttacataaa atagcccata
    32761 aataaagaag caatattgaa ataaatcagt acactcgtca aatcgctcaa agtcgtgata
    32821 aagggaccgc ttgctactgc tggatcaaat cctagacgat ccattagcat cgggattaaa
    32881 ctacctgcaa gattcgccac tgtgattgca aatagcatag ccatccctat gacaaacccc
    32941 aagataaaat tatgtttcca aaaacctacg attagaaaaa tcgtgatccc cgtaacagct
    33001 cctgtgacaa cgccggtcaa gacttcagcc aaaacaagtc gaacaaatcc atgatctttc
    33061 tcatcagaaa tcgcaagtcg acgaacagcc acagctagcg actgggttcc agcattaccg
    33121 gcagttcctg taatcagcga aatgaagact gctaaaatac tcgcttcact taccaagtct
    33181 tcatagtgac tgattaaaga agcggtcgac atacctaaaa acaacagagt gatcagccaa
    33241 ggcaaacgtc gagacgctgc ctttactgga ttttcattaa tttcttccac attgaccccg
    33301 gcaagtcctg aataatcgct agcagcttca tcatcaataa cgtcgatgat atcatcgact
    33361 gtgacgattc ctaacaaatg atcatcataa tcggtaacag gaagagctaa gaagtcataa
    33421 tctctaaatg tttgtgccac atcttcttgg tcgtcgccta catgaactga aagcactcgt
    33481 tcgctcatca gatcagaaat cattgcgtcg tcatcattca cgattaggtc gcgcagagaa
    33541 ataaccccta ctagctgacc ttcttgatta acgacataga tgtaatagat tgtttcagct
    33601 acatctgctt cgttcttcag cacgtacatc gcagagcgaa cagtttgatt ggctacgatc
    33661 gaaacaaatt cagttgtcat gatcgcgcca gccgtttcat cctcgtaatg gagaagttcc
    33721 ttgatttcac tggcatcgtc tgccgacatc aaacttaaat atttagcaat ttgcttttta
    33781 tccagcgtat taagcaagtc aacagcatta tctgtataca tctcagaaag catatcagct
    33841 gcatagctag gacgcatttc atctaggtat tctttcatat gttcgttgtc ttcttcaatc
    33901 acatcgaaca tatccgctaa ttctttaggc gacagatagg aatagatcag ctgcctgttc
    33961 tcttctgtaa gagattggta gaattgcccc tgctcataga tatgcatctc taaaaaactg
    34021 tttctgaatg cctgaatatt attttccctt aaatcttgag tcaactgggc aaatctttcc
    34081 tctaattctt gattttcatc caaaccaatc tcacctcgcc ttattcttca aaccgtctca
    34141 caactgcctc catgtctgcg gctaaaggct gcttgaactc cagcaactct tctgtaaatg
    34201 gatggtaaaa acgcagatca taacaatgaa gggcttgtct ttttattccc agatccattc
    34261 gcccgccata catatcatca ccgagcaggg cacagccaat tgcggaaaaa tgaactctta
    34321 tttgatgtgt ccttcctgta tgcagctgga tatcaactaa cgcgatttct gttagtcgct
    34381 tctctatcca gtactcagtc tgtgccgaac gtccttcatc agagaccatt cgcttcaata
    34441 gcgaagaata atctcgggcg atcggcaaat cgatatgtcc atgtgccgat aaatctgaaa
    34501 gccttccact gactaaggcc tgatattttt tcacaaactt cttttcacga agttgcatat
    34561 ctaattttgc gtgcgcaaat ccatgctttg caaaaagcat caaaccagat gtatcccgat
    34621 ctagtcgcgt caccacatgg attacttggt tttcgtaacc ttgtttttta taataagcct
    34681 ttacacgatt agccattgtc ccatttggat ggtattgggc aggaatagac gaaacgcctg
    34741 ctggtttatt tactaccaga acgtgttcat cttcatacac gatatcgatc ggtgtttcat
    34801 ccaaaagtac ggtttcatgt tctccttcag caggaatcac aatggttacc ttatcatcct
    34861 tagctagaga aaacaagaca ttttctactt gatcattaac tttgatttga ccgccttgaa
    34921 attttatttt cgctaacaga ccctttgaga tccctttttc ttttaaaaaa tattttactt
    34981 gctgagaatg atctttctca taatcccatg taaactgcat atcaatcctc actaataaag
    35041 gcatctttca cacgatgcca aaaatgcata tgtcgataag aagcaaaatg gatccgttct
    35101 tctgctattc gataataaat tgagcggata tttgcttttg cgacattcaa ttgatcaacc
    35161 gttacaagat aatcttctgt attttccaat tttatttcta gccattcatc atgggcaatc
    35221 acgatcggag aacccaaagt acgaaacact cgattattca gtgaagcaat ctctgccaac
    35281 tgaaaggcat tgatactagg atggatcaca gcaccgccga cacttttatt atatgctgtc
    35341 gaaccagtag gcgtagaaat cgtcaatcca tctcctctaa aactttcaaa cagctcgttt
    35401 ttgatgtata catcggctac catcgtacga ttggcccgtt taatcgttga ttcattcagt
    35461 gctaaaaaat gctggtccgg tgtttcatcc aaataactga tccgaacatc cagcaaggga
    35521 taactaacac tttgttcccg gttcgttcgt aagctgtcta ctagttcttc cagctcgtaa
    35581 tcccgccaat ccgtgtaaaa ccctaaatga cctgtatgca cgcccaagaa acgcacatca
    35641 ttcagacaat gactgtagtg atggaacgcc gacaataatg tgccgtctcc gccgactgag
    35701 atgaccaatt ctggctcatg atcatctcgt tgaatccctg cttgttctaa aagtgcgttc
    35761 aaacgttctg tcacttctat agtctttgct tctttattat gaacgatcgc tactttcatc
    35821 ccctgcctcc tctctttcgc tttctttctt caaaagcgtt cgatagtaga catcatctga
    35881 ttttcctctt ccatgggaga ataaccgctg tgcttcttgg atttcttcac ggatactgga
    35941 catctcttca tccaactgat aggcagcttc tgccgcacgc tgaagtcgtt cactcatttc
    36001 ttctggaaaa acgccttgat atttataatt cagcgaatgc tcaatcgttg cccagaaatt
    36061 catcgcaaga gtccgaatct ggatttccgc caaaattttc ttctcaccgc tgatcagctg
    36121 tacaggatac tcgatcacca gatgataaga acggtagcca cttgcttttt tatttgtgat
    36181 gtaatctcgt tcttggagaa ctttcatgtc ttttctttta cggatgatct caacaacttg
    36241 atggatatct tctacaaact gacacatgat ccttaacccg gcaatatcag acatttcttc
    36301 ttctaaacgt cccattggaa tatgccgtaa ttgtgatttt gttacaatac tatcgactgg
    36361 cttgactctc ccagtgacaa attcaatcgg gacatgtcga ttttgttcac ggaactgttt
    36421 gcggatcccc cgcagtttca ctttcaattc tgatacagtt tgttcatatg gtgctaaaaa
    36481 tgcttcccaa tctcgttcca tagctgatac ctctacttca ttaacattca ttgtttattc
    36541 taccatatat gcaaagtttt ttcgtactct tgatttatgc attgaccaaa aaaagaaaat
    36601 taggcaaaat agacaaaaag gagtcgatca aatgacacaa atgcttgaaa tcgaatataa
    36661 atctatgctg acaaagcaag agtacgagat attcatcgct cactatcaat taacagataa
    36721 agatttccgt gtacagacta atatttattt cgataccgtt gatgaacagc tgaagaaaca
    36781 aaattgtggc ctacggatcc gtttcgttgg tcatcaagct gaatatacat taaaaacacc
    36841 tgctgaaaaa gggcgtctcg aaacaacgga tacctttata acagaagaag cagaagcatt
    36901 tatcttcgaa aaacgtctgc ctcgttcagg cgcagttttt caaaaacttt ctgatttgaa
    36961 tatcgatcct gcttcattaa tccaaaccgg aaagcttaca acaaaacggg ctgaattccc
    37021 tattgaagaa ggacttttag ctattgatga aagctgggga gacaatctac acgattttga
    37081 actggaacta gaggtagggg atgcatctgt gggaaaaatt gcttttataa attttttaaa
    37141 acgtttttct ttaccttatc gtcctgctaa gaacaagatc caacgtatgc ttgaagctaa
    37201 aaattagaaa cctttcattt tcacaaggca cttgagttca tttttttact tttttctgtt
    37261 aagttatagg atgtgaaaga atctagtcac tagttgtata gaatctattt tgggggagat
    37321 tcatatgatc gaaatctatt tatttgttaa tcctttaggg gaatactgtt tcgaaatgga
    37381 acaacagtta ttacaattta tcgaagaaga gtacgggaca ccatcaaaag aaaagatgca
    37441 atttcgcttt cttcctttgg tcaatttgca gacaattggg gatgtcatgc aacaaaaagg
    37501 aatttctcag aacaatcttg aggaacgtaa tcacttattc tcaatgactt attctgcagc
    37561 attggactgc aaagcggcgc agtttcaagg aaagaaaaaa ggccgccgtt ttttgatcaa
    37621 attgcaagaa gcagtcggct gtaatgacat accatattct caagaacttg ctgaatccat
    37681 atttgaagaa atcggcggcg atttgaatat gtttaaagaa gatcgtcagt ctgacttcgt
    37741 taaagaagta ttcttttctg atcaaaaaat tgctcgggaa atgggcatca caaaacaccc
    37801 ttctgcagtc gtttacaatt atgcctgcga ccgtgatttt ggtgttttag tagaagtaaa
    37861 aggtacaagt agcctttgga gaatcacaga gctgtgtaaa acaacagata actcatatca
    37921 agtttttaat ggtgaacatt tacatacaaa tgattctcag ttccattcaa gaactagcca
    37981 tttacacttg ctttctaatt aaaaaaatac gtcgactccc ttacaagaga gccgacgtat
    38041 ttttttcgtt tctattcgag aaaatcaaaa atagaaagcc ttataaacga tcgatcaatt
    38101 cttctaattc atttagacgt tgttcaaaca tcgacattgc atcttcaata tatgcagcct
    38161 gtgtcatatc aactcctgct tttttcatta cttccacagg ataatcgctg ttacctgctt
    38221 ttaggtaagc caagtagttc tctagtgcct ctggttcctg gtttagtatt ttcttcgcca
    38281 gtgctgaagc agcagaaaag ccagtcgagt attggaaaac atagtaatta taataaaaat
    38341 gcggtatccg tgaccattcg aatttaattt ctgggtcttc ttcgactgct ggaccataat
    38401 attttgcatt caatttacca taactatcac ttaggtattc actagtcaat ggcacacctt
    38461 tttcatcttc cgtatgcatg aaatgctcga attcagcgaa ttgtgtctgg cggaaaactg
    38521 ttcctttaaa cccatccaag taatggttga gtacataagc ccgtacacga gggtcttttt
    38581 ctgtttccaa taaatactcc gttaggatat tttcattcgt tgtcgaagca atttctgcta
    38641 aaaagatgga gtagtcgccg tacacataag gctggttcga acgagtgaaa taactatgaa
    38701 cactatgtcc catttcgtgg acaagcgtaa atagctgatc cagcgtatca tgccaattca
    38761 ataaaatata tggatttgtg tcatagcttc ccgaagaata agcatcgctt cgtttccctt
    38821 tattttcgac aacatcgatc caacgttcag agaatgcttt ttctacgatg gccatgtatt
    38881 cttcacccat tggtttcagt gcttctaaag ctttttcttt tgcttcttcg tacgtaaagg
    38941 taattggagc ttcccctaag accggtgtat aaagatcata catgtgcagt ttttccactt
    39001 ctaataaacg tttccgtaat tccatgtatc gatgtaacaa aggcaaatgc ttgtttacca
    39061 cgtctaccaa agtatcgtat acactttcag gaatatgatt attgctcaaa gacgcttctc
    39121 tggcagagct gtaattacgg actttcgctt taaaattatg tccttttata tgtgtgccta
    39181 aagtagaagc aaatgtattt ctaaattgtt cgtaaacact gtacaatccc ttaaatgctg
    39241 cttcgcgcac ccttcgatcc gtgctttcta gcaactgacc atacacgcca tgagataatt
    39301 ggactatttc accattttct ccttcaatcg ttggaaaaac tagatctgca ttattcaaaa
    39361 cagcaaatgt atcacttgaa gcatcaaaga tttcacctgc tccagcaaga agagattcct
    39421 gctcagcaga taagacatgg gctcgattat ctactatttg ctggatataa tggcgataga
    39481 cttccaattt tggttcttct ttgaaatatt gccaaatctg gtcatctgac aattgcaata
    39541 tttctggttc aaaccaggaa acggcttcac taactttcga aaacagactg cttgctcttg
    39601 cataaagcgc ttggtaatct gtatttccag tgtcttgatc gtttttaagg tgcgcatata
    39661 cataaatgac ttcagtttgg cgataaaccc tcaatacgaa ttcaatcgca tttaaaaatt
    39721 gagaagcgcc ttgatcaagt gttcctttgt gtttttcaga ttgttttaac tcttctgata
    39781 attccaaata tttctcatca aactcttggt cgcttgaaaa gatcttggtc aagtcccaag
    39841 ttaaattttc aggcaattct tctcgttttg gcaactgctt tacttccatg aaatccactc
    39901 cttttcttcc cttagctcta tctatcaaaa tagatcatag tctaacatcc acatgtcaca
    39961 gcatctaact tttctactca ttcatgtgtc tgcttccatc aattttagca taactaaatc
    40021 ttgttttaca tagaaaaccg attcctagcc tatttttata cttactattt tttcaaagaa
    40081 gacaagtgta tgcattcgtt aaaaaactgc tgatagacca tctcttcatc aatcatagga
    40141 aaaagccatt cccttttatg ttcttttatt tggcaaagcc atgagttgaa atcaggaact
    40201 ttctggtttt ccaaggcttc ttcgtacaac attcgataaa gaaaaacctg ttcttgtaaa
    40261 taaaagaaaa accgactttt ttgatagatc cacgatgata gatgcaaaag atgtttatga
    40321 cgcaagtagc attgttcttg tatggatact gtattctttt gcttgtgaat aagttggcgt
    40381 gtcagccatt tcttcccatc ttcactcact ttttctattt ttttcatttg gttcatttta
    40441 atttcgttac tagtgaatat ttctttaagg tccctactag aaaaagtcca gcagctactg
    40501 caaaaggaaa tatgccctga tacagtctct gtaatatgat gatacaaata aagcttttgt
    40561 ttcttgagat cagctttcca gcaatgcact cctctttttc tattatagta gcagcaactt
    40621 ttttctatca atgagaattg cgattgtcca agaaaagaat taccaagtat ccaccatact
    40681 tggtaactgt tattcaaata attttgggtt ctttcaataa atctctggtg agacagtcta
    40741 gaacattgga tttctattgc tagattcgcc aacaaaatat ccggcctttg ccgcaacctc
    40801 ggcaaatagg cttccaaaaa tactggttct actgactgtc ccagccaatc catgaagcat
    40861 tcctttaact gtaagtgctc ttttgattct ctttcactaa agaccgaaca gttatctgat
    40921 ttttgatggg caaaatgagc acattttttc tttccctttt tcaaaatcac tctttttcga
    40981 cagccacgac aataatattt ctttcctttt tctgcatgtt tagcaaaaca gcacttatta
    41041 tattcattca ttgcaagcag catttttctc cctctgaact tatttacgtc taagaattta
    41101 tttacacgaa aaaagagggc gtgaaaaagc tattctgcat caagtaataa gaacagctaa
    41161 acaaaaatag ttcccaatat ttttcgtttg gttgggcttg ttaccgcaga tgcaagcttt
    41221 tgaacacatt tatacgaaaa aaagactgcg acacaatttt tgtcacagtc tcttactata
    41281 atgctgattt atacaaaata aaatttcgtt cgctcgatag cattacgcgc catcagacat
    41341 tgtccatatt cattcaatgt ttctggtgta cgatcgctct tttcagcaaa ctccaaaatt
    41401 cgagcaatct gattctctac atccgcttgg ttgagatttt ccatccagaa gtgaacgact
    41461 aggtaatagc gctcttcgaa taaatacaaa tcagtccaag cagactctag ttcgacatct
    41521 gttgcaagtt ggatcacttg ttcaaaatct tcaaaagcaa aagttgcttg tctttcctgt
    41581 tcttctaggg cgcttccaga taattcttcc tccggatcgc tgccaataat ctgctgacga
    41641 agaaagtcac tcacatcttc tgaatcaaca gtagaatctt ctgattgaga aatttcttca
    41701 tccggaagat ttttactgat aaacagttct aatccgtctc cttttggcaa aacttggaaa
    41761 gtgacagctt cgcttcctcg gaactcctct tcgatatcaa cttcttccaa aatactataa
    41821 aagaaatttt cgatttcttt gtgattgcca agtaaatcca aaaatgaaac gccccgttcc
    41881 tctaaatctt catgaccaat tagtacacgg atcgtatttt cattaatatg ttccatttcc
    41941 attttgctac acctcacttt gccacaatgt ttaatataga ttcattgtag cgaaatcagc
    42001 ttaaatgtaa agaaaaaaaa tctcgctgtt aagcgaattt aataaaacca gcggattttt
    42061 caacctttca agctatctta tttaactata acatttttgg cagacgaata ctatatagcc
    42121 gacttttcag ctaattttcc ctctctattt ttcgtgcaat catcatcttc tactaccttt
    42181 ttatacatct aatcaagaat actttttgta aaaacaaaaa aacttggaaa aactatcttt
    42241 gtaatagtct tt
//
LOCUS       DACDIE010000018        40583 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.018, whole genome shotgun sequence.
ACCESSION   DACDIE010000018 DACDIE010000000
VERSION     DACDIE010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.018"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            666..2276
                     /locus_tag="IV250_001074"
     CDS             666..2276
                     /locus_tag="IV250_001074"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAP8768373.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            2568..3437
                     /locus_tag="IV250_001075"
     CDS             2568..3437
                     /locus_tag="IV250_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAP8768374.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            3602..4873
                     /locus_tag="IV250_001076"
     CDS             3602..4873
                     /locus_tag="IV250_001076"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP8768375.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            4870..6165
                     /locus_tag="IV250_001077"
     CDS             4870..6165
                     /locus_tag="IV250_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAP8768376.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            6264..6527
                     /locus_tag="IV250_001078"
     CDS             6264..6527
                     /locus_tag="IV250_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAP8768377.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            6972..7421
                     /locus_tag="IV250_001079"
     CDS             6972..7421
                     /locus_tag="IV250_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8768378.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            7478..8896
                     /locus_tag="IV250_001080"
     CDS             7478..8896
                     /locus_tag="IV250_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8768379.1"
                     /translation="MKIVGVTACPTGIAHTYMSVEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            8896..9756
                     /locus_tag="IV250_001081"
     CDS             8896..9756
                     /locus_tag="IV250_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAP8768380.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            9930..10703
                     /locus_tag="IV250_001082"
     CDS             9930..10703
                     /locus_tag="IV250_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP8768381.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            10850..11215
                     /locus_tag="IV250_001083"
     CDS             10850..11215
                     /locus_tag="IV250_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HAP8768382.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(11363..12571)
                     /locus_tag="IV250_001084"
     CDS             complement(11363..12571)
                     /locus_tag="IV250_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8768383.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(12590..12868)
                     /locus_tag="IV250_001085"
     CDS             complement(12590..12868)
                     /locus_tag="IV250_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8768384.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            13061..13717
                     /locus_tag="IV250_001086"
     CDS             13061..13717
                     /locus_tag="IV250_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP8768385.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(14159..14956)
                     /locus_tag="IV250_001087"
     CDS             complement(14159..14956)
                     /locus_tag="IV250_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAP8768386.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(14953..16137)
                     /locus_tag="IV250_001088"
     CDS             complement(14953..16137)
                     /locus_tag="IV250_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP8768387.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(16162..17409)
                     /locus_tag="IV250_001089"
     CDS             complement(16162..17409)
                     /locus_tag="IV250_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HAP8768388.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            17624..19369
                     /gene="dnaX"
                     /locus_tag="IV250_001090"
     CDS             17624..19369
                     /gene="dnaX"
                     /locus_tag="IV250_001090"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAP8768389.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            19389..19703
                     /locus_tag="IV250_001091"
     CDS             19389..19703
                     /locus_tag="IV250_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAP8768390.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            19728..20324
                     /gene="recR"
                     /locus_tag="IV250_001092"
     CDS             19728..20324
                     /gene="recR"
                     /locus_tag="IV250_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAP8768391.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            20388..21032
                     /locus_tag="IV250_001093"
     CDS             20388..21032
                     /locus_tag="IV250_001093"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAP8768392.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            21047..21376
                     /locus_tag="IV250_001094"
     CDS             21047..21376
                     /locus_tag="IV250_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8768393.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            21390..22328
                     /gene="holB"
                     /locus_tag="IV250_001095"
     CDS             21390..22328
                     /gene="holB"
                     /locus_tag="IV250_001095"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAP8768394.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            22364..23188
                     /locus_tag="IV250_001096"
     CDS             22364..23188
                     /locus_tag="IV250_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAP8768395.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            23181..23528
                     /locus_tag="IV250_001097"
     CDS             23181..23528
                     /locus_tag="IV250_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAP8768396.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            23597..24469
                     /gene="rsmI"
                     /locus_tag="IV250_001098"
     CDS             23597..24469
                     /gene="rsmI"
                     /locus_tag="IV250_001098"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAP8768397.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            24578..25699
                     /gene="dinB"
                     /locus_tag="IV250_001099"
     CDS             24578..25699
                     /gene="dinB"
                     /locus_tag="IV250_001099"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAP8768398.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(25753..26355)
                     /gene="nrdG"
                     /locus_tag="IV250_001100"
     CDS             complement(25753..26355)
                     /gene="nrdG"
                     /locus_tag="IV250_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAP8768399.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(26670..28859)
                     /gene="nrdD"
                     /locus_tag="IV250_001101"
     CDS             complement(26670..28859)
                     /gene="nrdD"
                     /locus_tag="IV250_001101"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAP8768400.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            29185..29952
                     /locus_tag="IV250_001102"
     CDS             29185..29952
                     /locus_tag="IV250_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8768401.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            29942..31984
                     /locus_tag="IV250_001103"
     CDS             29942..31984
                     /locus_tag="IV250_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8768402.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            32265..32414
                     /locus_tag="IV250_001104"
     CDS             32265..32414
                     /locus_tag="IV250_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HAP8768403.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            32425..33939
                     /gene="dltA"
                     /locus_tag="IV250_001105"
     CDS             32425..33939
                     /gene="dltA"
                     /locus_tag="IV250_001105"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HAP8768404.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            33936..35141
                     /gene="dltB"
                     /locus_tag="IV250_001106"
     CDS             33936..35141
                     /gene="dltB"
                     /locus_tag="IV250_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAP8768405.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            35186..35419
                     /gene="dltC"
                     /locus_tag="IV250_001107"
     CDS             35186..35419
                     /gene="dltC"
                     /locus_tag="IV250_001107"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAP8768406.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            35422..36690
                     /gene="dltD"
                     /locus_tag="IV250_001108"
     CDS             35422..36690
                     /gene="dltD"
                     /locus_tag="IV250_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAP8768407.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            36831..37913
                     /locus_tag="IV250_001109"
     CDS             36831..37913
                     /locus_tag="IV250_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAP8768408.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            37913..38716
                     /locus_tag="IV250_001110"
     CDS             37913..38716
                     /locus_tag="IV250_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP8768409.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            38786..39526
                     /locus_tag="IV250_001111"
     CDS             38786..39526
                     /locus_tag="IV250_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP8768410.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            39537..40541
                     /locus_tag="IV250_001112"
     CDS             39537..40541
                     /locus_tag="IV250_001112"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAP8768411.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
BASE COUNT        13042 a         7107 c         8458 g        11976 t
ORIGIN      
        1 ttttgtcgca tctccttatt ttttacttat gtgattcgtt accttcaatg aatccctaca
       61 aggcactttt gttgaaaaaa aggtcatctt tgaaaaaatt agtggaattg ttttgttttt
      121 gatattattt aatagaaaag atatgccaaa aatccgagaa ctgtggacta cgttttttac
      181 gtaaaggagg taaataaggg tgaaagaaaa agattgtttc tacgataagc tatataaact
      241 gttgctgcaa gtaggaaaaa gtagttcttt gttaggtggg atattatcta tcataatgtt
      301 tgttttattt ttttatctat tatttcactt aaaatttttc agtaacgatt ttctgatttg
      361 cttaggttta acgttagcta tgtttatatc aagcttatta tgtgggttaa attttttttg
      421 cactcataaa ggacttaaga aataatttct caaaggatat cgaaaaagaa aaaattgtaa
      481 gcggatagta ttcatttgag tacttgactt tcccttagtc agcttgtatt atacaccttg
      541 ggcacccttt tagagggtca gaagtttaca gaatttcggc tccctattct tattgaagaa
      601 tagggagctt tgtttattgt ctgagacttt tcctgcacat aaattaaaaa ggagtttttg
      661 aacttatgac aaaatatatt tttgtgaccg gcggtgtcgt ttcatcaatc ggaaaaggga
      721 ttgttgcagc ttctttagga cgcttattga aaaaccgcgg cttaaaagta actatccaaa
      781 aatttgatcc atacatcaac gtggatccgg ggacgatgag cccatatcag cacggagaag
      841 tattcgtaac agatgatggt gctgaaacag atttggatct gggccactac gaacgtttta
      901 ttgacatcaa tttgaacaaa tactccaacg tgacgacagg aaaaatctat tctgaagttt
      961 tgcgtaaaga acgtaagggc gaatacttgg gagcaactgt tcaagttatt cctcatatta
     1021 caaatgagat caaagaaaaa atcatgcgag cagctaaaat gactgattca gatgtgatca
     1081 ttacagaagt agggggaaca gtaggggata tagaatcttt gccattcctt gaagcacttc
     1141 gtcaaatgaa agcagatgtc ggtacagata atgttatgta tatccataca acattgattc
     1201 cttacttgaa agctgctgga gaaatgaaaa ctaaaccaac gcagcacagt gtaaaagagc
     1261 tacgtggttt aggtatccaa ccgaatattt tggtggtacg tacagagcaa cctgtttctc
     1321 aaagcgtgaa aaacaaattg gcacaatttt gtgatgtcga accagaagca gttatcgagt
     1381 cgccagatgt agatacactt tactccatcc cattagtttt acaagcacaa ggaatggatc
     1441 aaattgtttg cgaccattta aaactagata caccagaagc agacatgaca gaatggaaag
     1501 aattagaaga acgtgtcttg aacttgaaga aaaaagtacg gattgcgctt gtcggtaaat
     1561 atgtcgagtt accagatgca tatatctcag tagttgaagc attgaaacat tctggtttcg
     1621 cctataattc agacatcgag atcgattgga tcaaagcaca agaattaacg agagaaaatg
     1681 tagaagaacg tttgaaagac gcagatggaa tcttggttcc tggtggattt ggtgatcgcg
     1741 gagtagaagg gaaaatcgaa gcgattcgtt atgctcgcga aaatgacgtt ccgtttttag
     1801 gtatttgtct tggtatgcag atggcttgtg tcgaatttgc acgtaacgtc gttggattag
     1861 aagatgcgtc atcagctgaa acgaatccag atacagcaaa caatattatt gatttaatgg
     1921 ctgatcagga aaacatcgag aatttgggtg gaactttacg ccttggatta tacccatgta
     1981 aaatcaaaaa aggaacgaag acggctgaag catatgatgg agccgatgtt gtgcaagaac
     2041 gtcaccgtca ccgctatgag ttcaataaca aatatcgtca attgtttgaa gaacaagggc
     2101 tagtcttttc aggtgtctca ccagataacc gtttagtaga aatcgtagag ctgccagaga
     2161 aaaaattctt tgtgggatgc caattccacc cagaattgat ttctcgtcca aatcgcccgc
     2221 aaaaattaat caaagcattt gtcggagcat cattagcaga tcacgaaaca aaataatttt
     2281 tgaacttgat agaaagagaa aagtagcaag agaaggggat aagcaatttt cttgtcactt
     2341 ttctcttttt ctgatttgtt attatttgta ggaaaaaaga tagaaagcaa aagacttttt
     2401 tcgattaaag cgctattgga tattttattt tttgcaaaaa acttcttgaa aattttcatt
     2461 ttcattgatt ttagtagaaa actaccgaaa tgtttggtaa aatagggtcg ttggttaaga
     2521 attgtcttaa caaatttagt tttacaaaac ttaggaggaa ttttattatg ccagtagtat
     2581 caggagctga atttttaaaa gctgctcgta aaggcggtta cgctgtcggc ggattcaaca
     2641 caaacaactt ggaatggact caagcaatcc ttgaagcggc tgaagcaaaa caagcaccag
     2701 tacttatcca aacatcaatg ggtgcagcta aatacatggg tggttacaaa atctgtaaag
     2761 atttaatcac tgatttagtt gaagcaatga acatcactgt tccagttgct atccacttag
     2821 accatggtga ttacgaagct gctttggaat gtatcgaatt aggctatact tcagtaatgt
     2881 tcgatggttc acatctacca tttgaagaaa acttgaaatt agctaaagac gttgttgaaa
     2941 aagcacatgc aaaaggtatc tcagttgaat gtgaagttgg ttcaatcggc ggagaagaag
     3001 acggaatcat cggtaacggc gaattagctg atattgaaga atgcaaacaa atggttgcta
     3061 caggtatcga ctacttagct tgcggtatcg gtaacatcca tggtcaatat ccagaaaact
     3121 ggaaaggatt agcttttgac cacttacaag caattgctga tgctgtaggt ccagacatgc
     3181 ctttagtatt acatggcgga tcaggtattc ctcaagatca aatcgaaaaa gctatctcaa
     3241 tgggtatctc aaaagtaaat gttaacactg aattccaatt atcatttgct gcagctactc
     3301 gtaaatatat cgaagctggt aaagataaag aaggtaaagg attcgaccct cgtaaattat
     3361 tagctccagg taaagcagca atcgttgaag atgctaaatc tcatatcgac tggttcggtt
     3421 cagctaacaa agcgtaagca tccacataac agatacgata tataggaatg gagaacgact
     3481 tttgaagtcg ctctccattc ttttttactt aaaacggcag accatgctcg tttctttttt
     3541 ttatctatga taaaatacaa gcatatccat gaaaaaagat taataaacaa ggtggaaaca
     3601 gatgaaaaaa atcgtaatca aaggaaaccg acctttagct ggtgaagtaa cgatcagcgg
     3661 tgcaaaaaat agtgcagtcg cattgattcc agctgcaatc ttagctgatt ccccagtgat
     3721 cttggaaggt gtgccggaca tacaagatgt tcattcatta attgaaattt tagagatcat
     3781 gggcgcaact gtccattttt ccaacaacat actagaaatc gatcctcgag aagtcgtatc
     3841 gattccgatg ccgcacggaa aaatcaatag tttgcgtgct tcttattatt ttatgggaac
     3901 actgcttggt aaatttggcg aagcggtagt aggcctgcca ggtggctgtt atttaggccc
     3961 tagaccgatt gacctacacg tcaaaggatt tgaagctcta ggtgctgaag tgacaaatga
     4021 acatggtgcc atgtatttac ggacagaaaa taaaggattg cgtggcaatc ggattttcat
     4081 ggacgtggtt tcagtcggag caacgatcaa tgtgatgtta gctgctgtaa aagcaaaagg
     4141 acagacagta attgaaaacg ctgcacgaga accggaaatc atagatgtgg caactttact
     4201 gaataatatg ggggcaaaag ttcgaggagc gggtacagat gtgatccgta tcgaaggagt
     4261 cgaaacatta catggctgtc ggcatttcat gattcctgac cgcatcgaag caggaaccta
     4321 tctagcatta gcagctgcag taggaaatgg tatcaaagtg aaaaatgtga tctttgaaca
     4381 tttggaaagc tttatagcaa aattgcagga aattggtgtc catatgaaaa tcagcgaaga
     4441 tgaaatcgaa gtttacccct cgaaagaatt aaaacccgca aacatcatga cctatcctta
     4501 tccaggattt gccacagatc tgcagcagcc acttactgcg ttactattga tgactacagg
     4561 cacttcagaa atcatcgata cgatctatgc gaaacgtgtg aaccatgtgc cggaactagc
     4621 acgaatgggc gctgatatta ccgtggaagg aaatatgatc atcgtgaatg gtccaaataa
     4681 acttcacggc acagaagtcg tggcttctga tcttcgggca ggtgcttgtt tagtgattgc
     4741 tggactattg gcagaaggga caacgacgat ttacaatgta gattatatct tacgcggtta
     4801 tgatcatatt atcgagaaac taacagcctt aggggcatca attgaaatga tagaggaagt
     4861 gggagctgaa tgagcgatta tttgacaatg gcagaactag agaacaaaac gttaaaagaa
     4921 gtttatagtt atgccaaaga atttaaaatt ccttattaca gtaagatgaa taaaaaggaa
     4981 ttatctctag ctgtgattcg tgcacaagca gagaaacaag gattttatta tatggaagga
     5041 atattggata tcgttggtca agatggttac ggttttttac ggccaatcaa ttatggacca
     5101 agtgcagaag atatctatat ttctgcctca caaatcagac gattcagttt gagaaatggg
     5161 gacaaagtgg caggaaaagc ccgtccacct aaagaatcgg aacgttatta tggtttgatg
     5221 cacgtggaaa gcgttaatgg aaaagatccg gaagaagcaa aagaacgtcc gcattttcca
     5281 gccttaacac ctctttatcc gcaaagacag cttcaactgg aaacaacacc gcaaagacta
     5341 tctacccgaa tgatcgatat cttttctcca atcggttttg gtcagcgtgg attgatcgtc
     5401 gcaccgccaa aagcagggaa aacttctgta ttaaaagaaa tcgcaaatgg tatcaccgaa
     5461 aactatccgg aagtagaatt aatcgttcta ctgattgatg aacgaccgga agaagtaacg
     5521 gatctagaac gaagcgtgaa aggcgacgtg gtatcttcta catttgatct gcagccgcaa
     5581 aaccatactc gagtttctga attagtattg gaacgcgcaa tgcgtttagt cgaagataaa
     5641 cgagatgtcg ttatcttgat ggatagtatt actagacttg ctcgggccta taacttagtc
     5701 gttccaccaa gcggacgtac attgagtggc gggattgacc cagccgcatt gtataaacct
     5761 aagaaatttt tcggggcagc aagaaacatc gaagaaggtg gcagtttgac gattttagcg
     5821 accgctttag tcgatacagg aagccgtatg gatgacgtga tctatgaaga attcaaagga
     5881 actggaaatc tagaattaca actttcacgt gaattatctg aacgtcggat cttcccagcc
     5941 atcgatatca aaaaatcagg tacccgaaaa gaagaattat tgatgccaag tgaagaatta
     6001 gaagaaattt ggaaacttcg aaaaaatatg actggagatt cattagaata tacggaacaa
     6061 ctgatccgct ttttgcgtaa aacaaaaaac aatcagcaat ttttcaaaga atttcaagat
     6121 gtctcctttg gtaaaaaaaa tccgaccaga aatctgaaaa gataattgca accttgactg
     6181 aaacatgtta agatgttaat gttgtaaatc gacaaatcaa ctctggttca gcaaatggag
     6241 cagggcaaag gagcgaaaaa atcatgaaag aaaacatcca tccagattac cacccagtag
     6301 tattcatgga ttcaacaact ggtttcaaat tcttgtcagg ttcaacaaaa acttcacaag
     6361 aaacagttga atgggaagac ggtaatacat acccagtcat ccgtgtcgaa gtaacttctg
     6421 actcacatcc attctacact ggacgtcaaa aattcacaca agcagacgga cgtgtggacc
     6481 gtttcaacaa aaaatacggt ctcaaagacg caaacgctgc ggaataataa aaacgctgat
     6541 aactcaacgt ttagcagact atctgatagg gatagtctgt ttttttatgt tttttgccaa
     6601 tttaaaactt cgtttgatgc tgaaaaagtc gtaggatcat aatgcacaaa acaatgaaaa
     6661 actagtatat agttttaatt atccacttga taaaaacacc ttatattaac gaaatgaaga
     6721 ggggttattt cgaagtaaaa aaccatttac ctaaaattag aagctaagct ttcctttcaa
     6781 ggaaggcttt ttgttttgtt atagacttgc gattgtttgt gagctagatc gttcatttta
     6841 atgacttata cacgcggatg atgggtcttt ttcctctgtt cattcataat attgaacggt
     6901 acagctagcg atgctatact gacctcagtt ccaagaaaac gttaacattt tttgtggggg
     6961 agaggttttt tatgaggttt gatcaattga tcgcagaaga actgattgtt ttcgatccat
     7021 cgatagattc aaaaggatca ttatttgagc gaatagggga tgtgttgaaa aaaacaaata
     7081 aggtaagaaa tgttcaaaaa gtcgttaagg cactattcaa acgagaggag gagatctcca
     7141 caggaataga agatggattt gggattcccc atgcaaaaag caaatatgtg acaaaaccag
     7201 cactgtgttt ttttcataca gccaaattaa cggactatag cgggatagat gaaatgccaa
     7261 ttgaatatat ttttgccatc gtagttcctg aaaaatcagc agatatgcat ctagagattc
     7321 tcagcacact ctccagacga ttgatgaatg cagattttag aaagggaata aaagaagcca
     7381 ctacgccagc agatatcatc agacagatta gtcaagaatg aaaatagtag agtctttgtc
     7441 cgagtatcga tagtgaagaa aaaggaggaa aataatcatg aaaatcgttg gtgtaactgc
     7501 ttgcccgact ggaattgctc atacttatat gtcggtagaa aaattagaag taacagcaag
     7561 aaatctaaac catcaagcaa aattcgaaac acaaggtgta aaaacagaaa atcagttatc
     7621 tgaacaagaa atcaaagagg cagatgcaat cattcttgca gtagataaag aaatcgaatt
     7681 ggaccgattt gccgggaaaa aagtaaagcg tgtctctaca agccgagcaa tcaaagaacc
     7741 gcaagtagta atagacgaag cattgagaga tattggcgta ttcgttgtgt ctaatgaaaa
     7801 agaaccagct gcaaatgaga aaaagccgac aatctataat catttcatga atggtgtgaa
     7861 ttacatgctt ccatttgtga tcgcaggcgg aatcatcatc gcactgagtt ttgcctttgg
     7921 gattacagcg gctgatccca actcagcaga ctataatgtg ttagcagctg cattttcaag
     7981 gatcggagga gatacagcgt ttgccatgat ggttcctgcg ttaggcgcgg ggattgctac
     8041 atcgattgct ggaaaagcag gttttgcccc aggaattgtt gccggattac tggcctctac
     8101 aggtggttca ggttttcttg gcggaatgat tggtggcgtg ctagcagggt atatctgtga
     8161 ttttctggca aataaaatta gcgtaaaaaa agaattttct gctatttatc agctgattgt
     8221 cgtccccttt ttatcgatct taattattgg gcttttgatg gtatttgtga ttgaacaacc
     8281 gatcacatgg gtgttggatg ctttgacaaa ttggctgaat agcttgggaa atacatccgg
     8341 gctattgttc ggattactta tcgggatcat gatggcagct gatatgggtg ggccgatcaa
     8401 taaatcgact tcgactttct cgatcggttt gatgtcagct ggagtaaatg caccgattgc
     8461 tgcttgtatg gctgcaggaa tgacacctcc cctaggtatt gctttagcca ctttattatt
     8521 caaacataaa tttaccaaag aagaacggac ttcaggtcaa tcatgctgga ttttaggcgc
     8581 ttcgtatatt acagaaggtg ctataccgtt tgcggtatct gacccgatcc gcgtgatacc
     8641 tagtttgatg acaggttcag cagtcgctgc agggatttcc atggcagtag gagttacttc
     8701 aatggcacct catggaggga tttgggtgat gctaattcca aatgttatga acaatttacc
     8761 tatgtatgtc ttggcaattg catcaggtac agtcactaca gcctttatgc ttggactatt
     8821 gaaaaaaaca gtgaaagaga aaaaacaaga caagtggacg aaaaatgaaa atctaacaaa
     8881 tgaggtggaa gcgtaatgtt atatacaatg aaagagttat tgaaagtggc taaagagaat
     8941 caatttgcag taccagcctt caatatttgc agctatgata tgatgaaagc aatcatcgaa
     9001 gaagcagaga gattagaagc accagtaatc ttagaaattc atcctgatga aatcgcctat
     9061 ttaagtgatg agtttgttgc ggcagtaaga gcctatgctt atacaagtaa actgccgatc
     9121 gttatccact tggatcatgg cggtacgata gaagatgtct tacgggcgat tcgaaacggc
     9181 tattcttccg tgatgatcga tgcgtctttg aaatcattcg acgacaatgt acagattacg
     9241 aaaaaagtgg ttgcgttagc tcatgcggta aatgtctcag tggaggctga gcttggaacg
     9301 attgggaata atggttctgc tgaaggaggt gctgaaacaa tcatttatac agatcctgat
     9361 caagcgaaga tttttgtgaa agaaacagga atcgatacac ttgctgtagc aattggaaca
     9421 gcgcatggct tgtatccgaa aaacagaact ccaaaattaa atatcgactt actgaaagaa
     9481 ttgaaccaac aaatcgatct tccatttgtt ttacatggcg gttcaggtaa tccggacgca
     9541 gaagtcagcg aagcagtaaa atacggagtt gccaaagtga atttgagctc agatcttaaa
     9601 agcgtctttt tcaatgcttt gagagaaata ttgagtgaaa atccatcgat gtacgagccg
     9661 aatgcaattt atccagcagc taataaaaag gtacaagaag tggtcaaaca taaaatggaa
     9721 attttgaaca cagtaggcaa ggctagttta tactaaaaat aaaaatgtgt ggtcaagagg
     9781 gtatgacaat agtcttgtca taccctctta accacacacg atgttcagaa gtagtttttt
     9841 cgaaaaattt cttcttattt ctcgaagcca atcacttctg tcacaacctc ttttttcctg
     9901 ttactatata aagtaaatgg ggtgatttta tgttgaatga agtggagaat agacagcagg
     9961 agattgtgaa ttatctaaaa aacaaccaat ttgcaagagt caccgatttg atcgagttag
    10021 taaattatag cgaagccacg attaaacgtg accttgtttt tctggaaaaa agtggattga
    10081 tccgtagagt tcgcggtggc gccatgctta ttgacaatcg aaaaattgac gtgccttacc
    10141 tgatgaagat caccaaatta gaagaagaaa cagaaaaacg taagattgca gatacagctt
    10201 cttctttgat tcgagatgat atggtcttat tcttggattc cagtacaacc tctttacact
    10261 tagtcagaaa cctagcaaga tttgatggtt tgcagatcat tacgaatggc atcgtcacag
    10321 ctgctatgtt gagtgaatat acaacggcaa aagtatctgt attgggaggg gcaattgttc
    10381 cgaaacgagc aacaatcaac ggttcaaaag cctataacga tgcattgtca tattatgcgg
    10441 atttagcgat aatgagttgt cgcgggctag atttcagcca aggagtaaca gaaactcatg
    10501 aaggagaggc aatggtcaaa aaagctttta gaaaacaatc agaccggctt gccgttttag
    10561 ttacacagca aaaaattgca cataaattca tgcatcaagc cattgctttt catgaaatcg
    10621 attatctggt gacaagtaaa gaactgacaa aagaacagta tgcgaaagta aaggaacacc
    10681 atatcgaatg tctgtacgga taaattatta cgtgaatgtt ctaagtggct tcctcatttt
    10741 tagataggac cttatctttg ctcgcttctc tcagccgatg gtataataat gaaaaggtta
    10801 tcttgttaga taatcttact tttttatcca ggagaaagga gtgcagaaaa tgaaacatgt
    10861 gaaggcgttg gtagtgaagg caatcatgat ctgggcgatt ctgtgggtcg tgctgacagg
    10921 tttatacggt gttagcttta tggattcaac gattgttggg gtaattattg tagtcatgat
    10981 ctatgtgcta ggggatctgt tgatcttacg taaagtaggg aacatcgcag cgacgatcgc
    11041 agatgctggt tcagctgcag ttatcttgtg gctctatcta tattttatga atgatacagt
    11101 cgatatttgg atgaaagtat tgattcctgc tctattgatc ggtgtggcag aatggttctt
    11161 ccacaagtgg ttgctaagtc aaggcatcgt accagatgaa cggaaaatgg aataaatcaa
    11221 cagttaaact attcttatcg ataaatgaaa gctttaaaaa taaatagatg tttaattaag
    11281 aataatcata aactattttt actcaaaaaa ccgctcagat tattttcaga tctgaacggt
    11341 tttttttatc tattcatcaa gatcaaattt catttttcaa tgattcaatc aattcgccct
    11401 ctgcttcatg actatgtttt cggccattaa caagatagag tgctgtacat atcaatggaa
    11461 tgactccggc aatcacatac aatcctctaa atgaagtaag agattttagc atccccatca
    11521 aataaggacc aacgcctaag cctaggtcta aaccaataaa gtaggtagat aaagcaatac
    11581 tgattcgatg ttcgcggacg atttttaaac aaacagcctg tccatttgac ataaacgtac
    11641 cataacctag gccgactaat gctccagaga taagcaatgt cacactattc gtcgttacac
    11701 tcagtaaaaa taatcctgct gttaagaata aatagctagg atacatcacg tattgttctc
    11761 ctttgacatc gaaaatcctt ccagtcaatg gacgtgtaaa tgtgatgacc aaagcatata
    11821 cgacgaagaa aaatgaccca gcagcaacta agtggattac ttttgtataa gaagataaaa
    11881 atgataaaac acttgaataa gaaagaccca tcaaaaaggc aattcctgta atgaaaagtg
    11941 ctttcttttc aataaaacta ttgatattcc atgagcgaag agatattttt tgttcttcag
    12001 ataatggaat attttttaca ggaaagacaa ggcaggcaat cgtaacaaag aagaccagta
    12061 caattgaaaa ccagataatg aaacgaaaat cagtcatatt cagcaaaatc attcctaaaa
    12121 atggcccaac agcggcagct aaactagtac ttaatccata ataattgatg ccttcccctt
    12181 ttttagatgc agggatatat gcagtaacga tagcatttgt agctgtagaa actgtaccgt
    12241 agccgaatcc attcaagaat cgaactaaat acaatgcccc gatagttggc gtataaagat
    12301 atgctaatgt tgaaaggaga tagaagatcg cgccgcctct taaggtaaac ttccgaccaa
    12361 atagttctaa ctgtttcccc atgaataagc gtgccagcaa tgttccgatg atatagatcc
    12421 cagaagcaaa acctgcttgg ctcaagctgg cgtgtaaggt gtcttgcgca atgactgcca
    12481 tgatgaccat caacagataa taaatcaggt agacaataaa attgatcaat gtgattccaa
    12541 taaaatattt attaaataat ttcttttcca tgtatgttat acattcccct taagctgaat
    12601 atgcttttaa tttttgtatt tgaacttcta catcgtgata attggatgga tagcgatcaa
    12661 caagttggcg aataaataaa tctcttgtag acataaacag atttaaaaat tcttcgcatt
    12721 cgtttgttgg attctcatcc actgctttga tcaattcgat cgagttttga tacgcatcat
    12781 ttaaaaagac taaagaagct ttcatatcaa atcttgtttg atatgctgtt tctaatcgat
    12841 taatgattgt gttcatccag taattcatgt tttttccctc tattcttaaa aaattttact
    12901 tctgcagtat aacgaataaa aaaaaggaaa aaaatcgcat aagtgatgga aaaaggtact
    12961 taacaaatgt taatatcatt agtaaaatac ctttggactt taaaaagttt taaagtactg
    13021 tatgatcttt ttagatgaaa gtttggatgg gggagcatca ttggatagta gagtcttaaa
    13081 ggaatatttg gataaaaatg attttccgat tatacgcaaa ggatatcata aatatttgac
    13141 attcgaaggg atggaagatt cccatacgta tatcttgaaa gagggaacgg taaaagcaag
    13201 tgttatttca aatgatggga gagaatttaa ccttcgctat atcaaagatc ttgaaatcgt
    13261 ctctctttta aaagacgaat attcacaatt catcgattcg ccgtataata ttcgagttga
    13321 atcagaatat gcagagtttt atcgaatcaa tcgtgtgcgc ttttggcaaa atatcaacca
    13381 agacccaaag ctacaacact atgtaaaaga gtattaccgc tatcgtctgc tttattcgat
    13441 gaaaaaaatg cagcaaatgc tattaaacgg aaagctggga gctgtttgta cgcaacttta
    13501 cgaattatat gatttattcg gtgtgcggac agaagaaggg atgctgatcg atttttcgat
    13561 cacgaatgaa gaaatcgcca aattttgtgg catctcaact gctagcagtg taaacagaat
    13621 tttaaaacaa ctgaaaaaag atggtattat caccgttaaa gaccatcata tcgttatttt
    13681 gaatatggac ttgttagaag acaatattat cttttgatga acaaagaggt tgtgaaatca
    13741 gcgttttgac ctgagtatta gagaaacaaa cggaaaaata gttcctcata ttttgccgtt
    13801 ttttgagcta ataccgaggg tcagctgatt gaacaccgtt taccaagagg ttgtgagcct
    13861 gccgtttaac tctgagtcac aaggaacaat ttcaaaaaac agcttttcat gtttgatgaa
    13921 tattgtgact tgtgaccgag gagttggtag gagaacaccg tttaccaaga ggttgtgaaa
    13981 aagctgttct gcatcaagta ataagaagag ccaagtaaaa acagtctctt atgtttttaa
    14041 cttggctgga cttatcaccg tagatgtaag cttttgaacg ccgtttattt gcaacaaaag
    14101 gggctgtgac acgatttttg tcacagccct ttttttgcga acattcattt agcaattttt
    14161 agaaacttct gccgccaccg ccaaaagtcc cgccaccgct agaatgggta gtacttccgc
    14221 caccgccgct tccaggaggt gggcttttgg ggattcttct tgtcgtgaca aaagaatttg
    14281 tcagacgatc ggttttttca gttaatttga tactggattt ttctcggaat ggatatttgt
    14341 agatgcctga tttcaattga taacgcgaaa cagtcacaaa ataaaagaca agactaagga
    14401 taagagccaa aacaacagca atgatgattt ctactgtcgt caaaacctta tagcgtgtaa
    14461 ttttgccagt ttcttcgtct acacgataat gtcctcctgg aactccgtct tccacatatg
    14521 ttgctgattt agacaggtaa gcttttgcag cattgtaata ggcagcattt gtcatctgat
    14581 cataaacatc atctaagatc gaatcgattc tgctatcagt caaataatcg atcatattac
    14641 cagaagtcga aatgtagatt tcacgctgtc ccatatctaa tagcaatact gagccatttt
    14701 gatcatttcc gactgcgtct cttaagtaat tatcagcaaa gtctcttggt tcttctgaat
    14761 tagatgtagt cgttacgatc aagactcgtc ctttgatttt ttcattcagc ttttctgcct
    14821 gttgttccaa agattggatt tgatctgttg tgaataaccc agcctcatca tttaccgtag
    14881 ctgtatctgc atgagtagcc accggccagc caacacttac tagcaaaaga aagaataaaa
    14941 aaaatttttt catcatatca agtaacctcc gatcagaaag aggatcgcta gcccagcgaa
    15001 aatcccaaga gcggtcagtc ccaatttttt ctggctgatc ggcaggacac cgctgacctt
    15061 tcctgtttgt ccattcattg cgtagtagta gactttttta cttgagtcat tgcttcgata
    15121 agtaacaagc cataccggca gtaatacgta attatttact tcgtttgtga ttgaagcaga
    15181 agttcgcaca ccggtcaatg ttgtatatcc atttgcggta tcttttaaca gcttctctga
    15241 atagtctttt aattcagatt gaatctgtga tttgattgcc tcatattcga tatctctttt
    15301 ttctgcttgg aacccggcta aatactggct tttaaaatca attgctttat ctaacgggaa
    15361 aggttgcacg ccttcgacca tcttttgttg cgtatttttt gataaggcat tttttactag
    15421 ttctttaaaa gaaagcttgc ctgctcttga aacagcaaat tgcttcgttt ctgtgtattc
    15481 gatgtcacct atccgccata tgcggataac tgtaccattc gcctgcatgg caccatctac
    15541 ttcagcatct gtagcccaat aagggaaata cacacctgtc aacttatcga tttgatcctt
    15601 attgaaaaaa gctttgggaa caaaccattt tttcttggtc caagcgagaa atctgttgat
    15661 tgcttcttct ttctcgacag caaaaggaag tactttattt ggcaagaatt ctccgcttaa
    15721 acgtcccgat agtacgacgg gattatggca gtagtaacaa taagtcgcag cagttgtcgc
    15781 atcagtcacg atctctgcac cacagttggg acaaagaaaa agatctatcg ctgcggtatt
    15841 atctgcagat tcgtccgctg tatctgaaaa accgcctgcc tcgtcaaccg ctttgcgctc
    15901 ggcttcattc atatcggcaa gctgttcttg ggctgtaaaa gtaaaatctt cggtaggtga
    15961 aggttcttct ttgctttctt cgccagccat acgcgcttct ttttgctttt gctcatattg
    16021 gctgacttct tcttctgtat agatattcag gcagtattcg caatgaaatt tttggtcttt
    16081 gggatcaaaa gtcaacggac cgccgcaatt aggacattta tgtgtaagaa catccatcag
    16141 ctgcacacct ttctaaataa attaattaac ggggattcct ttaaacggtt taccacagtt
    16201 tggacagaat ttagggatgc cgttggaaag atccacgatc tcattgcatt cactgcatcg
    16261 catttgtagc ttaggctgaa cgttttctac aggtttggca gctccgcagt tggaacagaa
    16321 ttttccggaa ttttctgtcc cgcaaactgg acatgtccaa gtatccgcat ttccttgtgc
    16381 agtttgttga ttttgttttt ctgcttgttg tttgatttgt tcttggttat tttgagctgc
    16441 ttgagaaaga taagaaccat ttgcattcat tcccatacct acacccataa agccggtcat
    16501 tgctcctgct tcattttttc cggctgcttc cataccacgt gcaatggaac cttgcacgta
    16561 tccttcacgt acgctaggat cacctagcat cgccccttcg ttacgcatat tgatcagttt
    16621 cacagaatca tcggtgtaag aaatgctggc tacagccaca gaaacaatct ccatcccacg
    16681 taattcacgc caagaatcat ccaatgcagt atccatgtat ttgcttaatt ccaaactctt
    16741 agatggtaca taagaaatcc gttgaccatc tgcagacatc tgattgattg ctgattgtag
    16801 cgctgttagg aattcagcta aatactgctc attgatatca ttgatttcca cttgtgtttt
    16861 gtttttaggg attgcgtttg tataaaataa aatcgggtca acgacatgaa tagaataagt
    16921 gccatgtgcc cgtaagaata attctgcgtt atagaaattg tcaaagtagt tcaacggcgc
    16981 gcttgtaccg aatttgattc cctttatttc ttggagattg atataaaaaa cttgttgttt
    17041 ctgcggtgtg acgccgccaa atttgaatcg gtcaaaagtt tcagcaattg cttctttcag
    17101 tgttccattg aacatggaag gagctgcgtc atttttaatg gtgtaataac cttcctcagc
    17161 ggtataatcg atgatttttc caccatcgac caaaagcatc atcatgtttg gatagacatg
    17221 gacgactgtt ccgtctgtta aaacatcttc tgttccttta cgattggagc cacgtttgtc
    17281 atctttgcgg actttgacac cttttgtcat gaccgttgta tcactcatat tgtctggttc
    17341 gatgacttct agccattgat ctgccagacc gccgccaacc atacttgttg ctgctttgat
    17401 aagacccata aaaaattcct cctctgattc agctagtgcc aaacaattat tgtttattta
    17461 ttataccata ggaagatgtg aaacttctcg gactagagac ggaattgaaa tggaaaattg
    17521 cgtattgttt aaatacctga aaaagccgag aaggatagta gtttttttac ctaatatcac
    17581 gtataataag agatagtgaa ttttttaaaa aagggggatc cttatggcat atcaagcact
    17641 ttatcgagtc tggcgttcgc agcgtttcga agacatcgtt ggacaaaaag ctgtgacaca
    17701 gacactaaaa aatgctattg tttcccacaa aacctcacat gcctacttgt ttacaggacc
    17761 cagaggaacc ggaaagacga gtgcagcaaa gattttcgca aaagcaatca actgtccgaa
    17821 cagtaaagac ggtgagccat gcaacgaatg cgaaatgtgt cgatcaatta ctgctggtac
    17881 gcaagaagat gtgatcgaaa tcgatgctgc cagcaataac ggagtcgaag aaatccgatt
    17941 tatccgtgat cgggcaaatt atgcgcctac aaaggcagcg tacaaagtgt acattattga
    18001 tgaggtacat atgctttcaa caggagcttt caatgccttg ttgaaaacat tggaagagcc
    18061 aaaagaaagt gtcatattca tcttagcaac gaccgaacct cacaaaatac cagctacgat
    18121 catttcacgg actcagcgat ttgattttaa acggatcaat acggcagata ttgtcgaaca
    18181 tcttgaattt atcttgaaag aatcgactat cagctatgaa ccagctgcat tgaatgtgat
    18241 tgcccgttct gcagaaggcg ggatgcggga tgcattgagt attcttgatc aggcaatctc
    18301 gtttagtgaa ggaacaatta ctttagatga tgccatgcaa gtgacaggta gcttaactga
    18361 cgaaatgatg gacagttact tgagtgcttg cgtgaaaaaa gaagtaccag aagcattgga
    18421 aacattgaac caaattctcg cagccggcaa agaatcacgt cgctttctag aggatatgtt
    18481 acaatactgc cgtgacttgc tgatgtatca gcaagcacct aacctagttg aggaacaatc
    18541 gagtacgatc agaagtacat ttacagaatt ggcacaaact gtttcagcgg atcagctgtt
    18601 ttcaatgata aagattttga gcgatactca aaatgaaatt cgttttacca atagtgccgc
    18661 tatttatttg gaagtcgcta cagtcaaact tgcacagcta ccgtctgctt ctgttccttc
    18721 agcagatgta gcatttgcag atgatcagat agtaaaacag ctacaacaag agattaagga
    18781 actaagaaac gaaatcaagg aactacgcag taatacagga attgagaata caaaagtaca
    18841 gccggtacag caaacaaatc ataaaaaaca gacaagtacc tatcgtgtac cgacggaacg
    18901 agtatataaa gttctgcggg aagcgacaag acagcatctg aaccaagtaa aagaagtatg
    18961 ggaagacttg ctgatgagtc tatctgtcac tcagcgagca atgcttaaag caagcgaacc
    19021 agttgcagct ggacctacag gactagtgat tgcgtttgat tatgagattg tttgtcaaag
    19081 agcagcaaat gacgaagaat tgcagctttc ggtacacaac aacttaagcc gaatgatcaa
    19141 agattatgcg cctgatcctg tttatattac aagagaaagc tggccagaac ttcgaagaaa
    19201 ttatttgacg caggcacaag aagagggaac agaaaactct tttgcttctg aagactcaga
    19261 aggagaaatc gagttgcttc ctcaagaaca agaagtacca gaagaaacac ttgtagtaaa
    19321 agcagaggaa ctgtttggct ccgaagctgt tacagtggta gatgattaat aaaaaggagt
    19381 agataattat gatgcgtgga atgggcaata tgcaaggcat gatgaaacaa gtacaaaaaa
    19441 tgcaaaaaga aatggcagaa gcacaagagc tgctaaataa aacagagttt acaggtaaag
    19501 ccaccaatga attggtcaaa gcggttttta caggcgatcg tcgcatgaaa gatatccaaa
    19561 tcaatgaaga agtcgttgat cctgatgata tcgagatgct tcaggatctt gtcgtaatgg
    19621 cagtcaatga tgcgatcgca caaattgatg tggaaactga gaaaaaatta gggaaatacg
    19681 caaaaggttt gccaggattt tagttttaaa ctgaaggagt aatgaatatg caatatccag
    19741 aaccaattgc gaaactgatc gacagctaca tgaaacttcc aggaatcggt cagaaaacag
    19801 cgacaagact cgccttttat acgatcgata tgaaagatga agtagtaaat gaatttgcta
    19861 aatctttgtt aagtgtaaaa cgcgatttgc atttttgtag tatctgcgga aacatcacag
    19921 aagaagatcc ctgcgaaata tgtagaaatc cttcacgtga tcgaagcgtt atcctagtgg
    19981 tcgaagagcc gaaagatgta atggcaatgg aaaaaatgcg agaatatcac ggactttatc
    20041 atgttctcca tggtgtgtta tcaccgatgg aagggacagg tccagaagac atcaatattt
    20101 cttctctgtt gcagcggctg catgatgcag atgtaaaaga agtgattatc gcaacaaatg
    20161 caacaacaga gggagaagca acagctatgt atctctcacg tctgatcaaa cccgcaggta
    20221 tcaaagtgac tcgtctagca catggtctgt ctgtaggctc tgatatcgag tatgcagatg
    20281 aagttacttt attgaaagct gtcgaaggaa gacgtgagtt ataaatttct aattaagtgt
    20341 tttttcagat acaatataaa agcagcacaa gagatggggg caacaatgtg aacggactat
    20401 ttattacaat cgaggggcct gatggtgcag gaaaaaccag catcttgaat gaactttacc
    20461 ctcttttgaa agaagtagca aaaacagaaa tcattcaaac aagagaacca ggaggcattc
    20521 cgattgctga agagatccga gcagtgatcc ttgatccaaa aaatgatcgg atggatgaac
    20581 gtacggaagc actgctttat gcagcagcaa gaagacaaca tctagtcgaa aaggttctgc
    20641 ctgctctggc aaaagggaaa atcgttttat gcgatagatt tgtcgacagt tctcttgctt
    20701 atcaaggtgc aggccgacgc atcggcgtta ccgagattgc tcggttgaat gagtttgcta
    20761 cagaaggtac tacgccggat tttacccttt atcttgacgt agattcagac accggattaa
    20821 gaagaatcaa gaaaaacaga caaaatcaaa tcgaccgttt agattcggaa ggtttggaat
    20881 tccaccagcg tgtacgtcat gcttacttaa agctggcaga agagaatccg gaaagaatcc
    20941 acaaagttga tgcaagaaaa agctttgaag aagtgctgca aacaagttat catacaatta
    21001 ttgaacagta tccgcaattt tttgaaaact aggaaggtga acgtttatga aaattatttt
    21061 agcgattatc caagacaaag acagcaaccg tttatcaaat gaattgattg atgcaaatat
    21121 ccgtgccaca aaactatcat cgacaggcgg cttccttaag gctggaaaca gtacgtttat
    21181 cgtgggtatt gaagatgacc gtgtagaaga agcattggaa atcatcaaaa aaacgtgtga
    21241 atcaagaaag caatttgtat ctacacctgt tacactggat atttcgatgg atgggggcgt
    21301 cccttatcca gtagaagtag aagtaggcgg tgccacagtc ttcgtcttac ctgttgaagg
    21361 attccatcaa ttttaggagg agctgagcta tgaatcaaga aataaacttg gctcaaatgc
    21421 aaccgatcgt gtatcagcag cttcaacgga gttttgagca tgggcgtatc gctcatgctt
    21481 atctttttga aggggagaaa ggaacaggaa aacatgaagt aggtatatgg cttgcacagc
    21541 acctgttctg tacccaaatg aaagacaatc tgccttgtgg aaaatgcaac aattgtcagc
    21601 ggatcgaaaa gaaagaacat ccagatgttt tggttattga accggaagga caaaccatca
    21661 aagtcgatca gatcagacgt ttgcagacgg aattcagtcg cagcggttac gaatcaagaa
    21721 aaaaagtttt tttgatcaaa gaagctgaaa aaatgaatag cagtgcggca aacagtttgt
    21781 tgaaattctt ggaagagccc cctggagatt ttttagcggt gttagaaaca gatgcagtag
    21841 gaagaattct ccctactatt caatctcgtt gtcaagttct gcattttcaa gaactttcga
    21901 aagaagcact gatccatcga ttgcaagaag agcagattcc attggaaaaa gcaaaacttt
    21961 tggctttctt aacaaacagt ttaggaaaag cagttgaaat ctctcaagat gaatggttta
    22021 atgatgctaa ggacttgatt tatcaatggt tcgtttactt gcaaaagcaa gatactcaag
    22081 cctttatcta tgtccaaaaa aaattggtaa aatcatttaa agaaaaaaca caacagttta
    22141 caggattatc tgtcttgatg ttttattatc aggaagcaca gcgtcaagca gtagcaaacg
    22201 gcgaatatgg caaagtgaaa cagataaacc agataattga acggatattg atcgcagagc
    22261 agaaactacg cagcaatgtc agttttcagg ctgttgcgga acagttcgtt ttgcatacga
    22321 ttttttagct tgccgcaaag gatgatgatc aaggaggatg gaaatggtag aagtagtagg
    22381 tgtccgatat agagatgcag gtcatatcta ttattttgca ccaggagact ctgaatatgt
    22441 ttatagtgac aaggtattag tcgaatctca gcaatcctta catattgcta ctgttgtcct
    22501 tcctaaaaaa gagattgatc ccaatgacct tcctgaagct atccgtccga tcttgcataa
    22561 agcgtcacaa gctgaattag aaaaagacaa aaaaaacaaa aaagatgctt atgatgcatt
    22621 gaaaatcgca aaagaaaaag tgcagttgcg tgagttggaa atgaaaatgg tcaaagccga
    22681 atacacattt gatcgaaata aattgatttt ttctttcaca gcggacgggc gaattgactt
    22741 tagagaattg gtaaaagatc tggccgctgt atttcgtact cgtattgagc ttcgacaaat
    22801 tggtgtccgc gatgaagcaa agataatagg cggaattggt ccatgtgggc gtccattatg
    22861 ctgttcttct tttttaggcg atttcgtgcc tgtatcaatc aaaatggcaa aagatcaaag
    22921 tttatctttg aatccaacaa aaatttccgg tttatgcgga cgcttgatgt gctgtctgca
    22981 atatgagagc gaagaatacg agacagcgaa aaaagaactc cctgactttg gaaaagtaat
    23041 tgctacacct gatggtaaag ggaaagtcgt agggttgaat ctgcttagcc gtatcgttag
    23101 tattcggctg actggacggg aaaatcctgt cgaatatgaa tgggatgaat tgaaagaatt
    23161 tatccaagta ggtgagatga atggataaga gatcattata cgacggattg aatcaattag
    23221 aatcagagtt gagaaataca ttaacagaac ttgttgaaat gaagcatgct ttgcatgaga
    23281 tcgtagaaaa aaatacaact ttggagatgg aaaatcaacg tctgcgggaa cacctgcggg
    23341 aattgaacca agttgcacaa gctccagctg aaaatgtgaa gcaggaactt tctaagtctc
    23401 gtatgaatct tgaaaaaatt tatgaagaag gattccatgt ctgctacgat ttatatggat
    23461 caagaaggga caatgacgag ccttgtgcgt tttgtctaga agtgatctat cgcgaaagcg
    23521 gtcgatgacc aataacaaga ggagactgga gcggagaaat actgctccag tctttttatt
    23581 aaggagattg tgacagatgt ataatcaaaa aagtttcaag ggaaatccgg tatatgggga
    23641 tttatatctt gtaccgacgc caattggaaa tttagaagat atgagctaca gaagtgttcg
    23701 tatgctgcaa gaatccgatc ttatcgcaag tgaggatacg cgtaatacac aaaagttatt
    23761 gaatcatttt gaaatccata caccccaaaa aagtttacat gaacataatt ataaagaacg
    23821 tgtccctcaa ttgattgctt tgttaaaaga aggaaaaacg attgctcaag tcagtgacgc
    23881 aggaatgcct tccatcagcg atcctggaca tgagctagtt ttagcctgca taaaagaagg
    23941 gatttctgtg atctctatcc caggacctac agctgggatg acggctttga ttgcttcagg
    24001 gctagtgcct caaccatttt tattttatgg cttccttcca cgtaagaaaa aagaacagaa
    24061 agaaacattg aaaaagctaa aagaacagac agttacgctg attttttatg agtcacctta
    24121 tcggatcact gctacattga cgaatttctt agaaattttt ggaaatcgcc aagtcgtttt
    24181 gtgtcgggaa ttgacaaagg tccatgaaga ataccttaga ggaagtacgg aagagctact
    24241 cgcttatatc gaagaacatc cagtaaaggg tgaatgttgt ctgcttgtag aagggaattt
    24301 gttcgaaatg ccagtgacgg aagttgatga agagttggga acgttaaagg aacaagtaga
    24361 agcaaagatc gcttcaggcg aaaaaccaaa tgctgctatc aaagcagtag cgctgaaaaa
    24421 tgggctgaaa aaacaagaag tctaccgtca gtaccatgaa ttagactgag atagtataag
    24481 ttgtctataa tgaaagacta atgaggaaat cagctactaa cttttcaaat ctcttgaacg
    24541 cgaacgaatg ttcttgtata atgaataaga ggtgaggatg atggagctgc aatttccttt
    24601 acataatgat acatcaagaa aaatcattca tattgatatg gatgcttttt tcgcttcggt
    24661 tgaagaacga gatcatcctg agtttcgcgg acatccatta gtaattgcca gacaccccag
    24721 tgacactggt gggcgtggag tagtgactac agcaaattat gaagcaagaa aattcggaat
    24781 tcattcagca atgagtgcac aaaaagcgta tgagctttgt ccgcaagcca tctttaagcc
    24841 tggcgaccat cataaataca gcgatatttc caaacaagta cgggaagtat tctatcgtta
    24901 tacagatatt gtggaaccag ttagtatcga tgaagcgtat cttgatgtga ccgaaaataa
    24961 aatcaattgc aagtcagcca tcaaaatcgc ccgaatgatc caagcagata tttgggagag
    25021 tgtccagcta acatgttctg cgggagtaag ttacaataag tttttggcca aattagcatc
    25081 agactatcaa aaacctagag gaatcacggt agttgcccct caagacgctg tgccattttt
    25141 aaaagctttg ccaattgaaa aatttcatgg ggtagggaag aaaactgtac ctagaatgca
    25201 tgagctaggg atttatacag gaaaagacct atatgaatgc acagaaatga tgttgattcg
    25261 aaattttgga aaaatgggct attctcttta tcgtaaagtc agaggaatcc atgattctcc
    25321 agtaaacgtt acaagagagc gaaaatctgt gggaaaagag catacttatg ggcagccgtt
    25381 gcaaacggaa gaagcagtgc tgactcagtt gcgccaacta gctgaaaaag tagaagaagc
    25441 actgagaaga gtccaaaaac atgggaaaac agtagttttg aaggtcaggt acacggacta
    25501 ttctactgtg accaaaagag tgacattgcc agaatatatc tataaaaaag aagcactctt
    25561 ttatcaagca agccttatat gggaggaaat cttgggagta gaaaaaggaa tccgattgtt
    25621 agggattact ttgacgaatc tggacccgat gacttatgaa aacatcgtcc ttccattatg
    25681 ggaaaatcaa gaaatataaa aacagctttg agagttgaaa ccggaaatcc gttttctctc
    25741 ccaaagctgt ttttatatca agtcttgctt tttgatttgt tcataggttt ctgttgcatc
    25801 cgtacatttt tcccaaatca caacttgatt ggattctaaa gatttttgga cgtcaatgat
    25861 ccgctggttg ctacttccgc gaaattgtag ttttaggtca cgtttgctta attcaaagcg
    25921 tccgtcaaca aggatatcga tttgcgaaag gagttctaat ttatcttctg aatcttgtag
    25981 caattcatca aaggtatagc ctgtccatga ccagatatct tttgtcgtac cgaactcttt
    26041 tcggatacgt cgtacgactt gcagacaaac atctgtattc aaaaaaggtt cgccaccaag
    26101 taaagttaat ccttgcacgt aatcgttgcg caagtcagcg atgatttgtt cttctaattc
    26161 aggtgtgaat ggtttgccat atcggaaatt ttgtgcagct ttgttgaagc agccttcgca
    26221 ggcaaataag cagccactaa cgtaaagact gttgcggaca ccttcaccat cgacaaaatt
    26281 aaacggtttg tagtcagcaa tatagttttg gctgtagtct tttgctaacc attcttttgg
    26341 ttttggattt ctcataaaaa aacttctttc atttaaggtt caaagatata gctattgaag
    26401 ataaaatcta ctcgaccaca taaataagaa caaactgacc taaatccatt gttcaagaaa
    26461 gcttgttctt attttgatag agggcgttca aagatacaaa gccatcgaaa gagggtggtg
    26521 aaaaaagact tgtcatagcc tcttattctg aataaacggt gttcaaaagc ttgcatctgc
    26581 agtaataagc ccaactaaac gaaaaatatg aggaactatt tttgtttagc tattcttatt
    26641 acttgatgca ggacagcttt ttcacaacct catttcatgt gtttcacacg tgaggaaatt
    26701 tctttatggc ggccatgtac catcggacga gcttgcggat ttcctaaata gccacaagta
    26761 cgtttgacta catcacatgt ttttggatca tgatttccac attctggaca ttcgaaccct
    26821 cgttctgtcg gtttgaaatc accttcgaaa ccacattgat agcaatgatc gattggcgta
    26881 ttcgtaccaa gatacccgat tttgtcatat gcaaaatccc atacagcttc taaagctttc
    26941 ggattttgtt ggagcatagg gtattcacag tagtggatga atccacctga acaatatttt
    27001 ggataatcct tctcaaattc caatttttca aatggcgttg ggtttttacg cacatcatag
    27061 tggaaactgt ttgtataata ttctttatct gtgatatttt cgatgatgcc gaatttttct
    27121 gtatccaaac ggcagaaacg gtcagttaga ctttcacttg gagttgaata gacactgaaa
    27181 tgataaccat attcatcccc ccatgcatct gcattagctt ttaattcttt gacgatatca
    27241 agtgtgaaag cttttgcttc tgggtttgtt tcccattcgc caccgaagaa agctgaagca
    27301 acttcataca atccaatgta gcctaacgat acagtagctc gacggttttt gaataattca
    27361 ttgacagaat cggtacgtga aagacgtttc ccgaaagcac catacatata taaaattggc
    27421 gcatttgctg gaatggcttc tttacaacgt tctacacgat agagaagcgc atctttcatg
    27481 atttctaagc ggtcagacag taactgccag aatttattct gatctccttt tgattcgatc
    27541 gcgattcttg gcaaattcaa tgtaacaaca cctaaattca ttcgtccgac atttacttct
    27601 tgaccatttt cgtctttcca cccttgcaag aatgaacgac agcccatagg tactttgaaa
    27661 cttccagtta attcaacgat tttatcgtaa ttcagaatat caggatacat tcttttcgtt
    27721 gcacattcca atgctagttg tttgatgtcg taattaggat cttcagggtt taagttcaca
    27781 ccttttttca aagtgaagat cagtttaggg aagatagctg tccgcttttc actccctaat
    27841 ccattgattc gaatctgtaa aattgctttt tgaatttctc tttcaaacca gttttctcct
    27901 agtccaaatc ctaatgaagt gaaaggagtt tgtccatttg aagtaaataa ggtattgatt
    27961 tcatattcca aactttgcat agcatcgaag atatcttttt tggtttttgc ttttgcatat
    28021 tctttttggc gttctggcga atcgatccat tcttccgcgt cttttagatg ttttttatag
    28081 ttcaattctg caaaaggagc aagcaattca tctgttcggt cggcagaaca gccaccatat
    28141 tgacttgacg ctacattggc aataatctgt gagatttgtg ctgtggcggt ctggatagat
    28201 tttggcgatt cgacttcggc attgccgatt ttgaacccat tgttcagcat gcctttgaaa
    28261 tcaatcaagc agcaattcgt catcggtgta taaggatgat aatccaaatc atggtaatgg
    28321 atatcgcctt tttgatgcgc atttgctata tgtggtggca acattttcag tccgattgat
    28381 tttccaacga tcccggctgt cagatctcgc tgtgtattga aaacattact gtctttattt
    28441 gcgttttcgt tcacgacagt ttggtccttg ttgattagct tttcaatcga aaagttgata
    28501 tctgttgctt tactgcgagc aaaatctcgt ttggtacgat aattgatgta ctcttctgct
    28561 aattcatatt cgtttttttc tagtaatata tgttccacga tgttttgaat ctcatagatt
    28621 ttgacatttt cagcaaaccg gcttttgatt tcttggacaa ccaagtctgc aatcatttga
    28681 attttttcat aagtaagtgg tgataatgag ccgtgaattt tcttttctgc tttgatcagt
    28741 gcttcattga tcttttgttc atcgaaatca acatggcggc catcgcgttt gatgactttc
    28801 atctgggatg tctctggctg ctgagtaacg acagtttttt cttcataatt tactttcatt
    28861 atcttcaact cctgctaaat tgttcttgtc tatcataaaa caaaatcggg gaaggagcaa
    28921 ctaaatatcg gctatatata gtattcaatg tgctgtataa ttggatttct atactatatg
    28981 tagtgtatat tttattgtga aaaaaggtga aaacttgtag ctggtggatt tgttttttgc
    29041 atatttaatt ttgctgaata aaataattga ataaaatttt ttattcagtc caagtaaaag
    29101 tgacaaaaac ataagagcaa attattcgta gatatgctac tatgcaaata aatagaaatg
    29161 aaaaatgaaa gaggaggaga agaattggaa aagatgatcg aagtgaaaca tctaagtaaa
    29221 agctacggca gcaaactttc tcccgtcaaa gccttagata atatctcatt cacagtagaa
    29281 gaaggcgagt ttgttgggat tatgggccca agtggtgcag gaaagacaac gctgatgaat
    29341 atcttgtcca cgatcaattt gcctacgatg ggcagtgtct cgatccaagg aaaagaaatc
    29401 acaaaaatga aaaacagtga gctcagtgac tttcgtcgaa aaaaactagg attcattttt
    29461 caagagttca atttgatcga taccctaaat gcaaaagaca atatcctatt gcccctagca
    29521 gttgaacgga tgactaaaga ggaaatggaa aagagagtaa gacatgtagc tcaattgttg
    29581 aatatagaag aactgctcaa acggtatccc gatgaacttt ctgtcggaca gagacaacga
    29641 attgcagctg ctagagcact tgttgttcag ccacaagtga tttttgcaga tgaaccaact
    29701 ggctctttgg actcgaagtc ggctacagaa ttgttgaact acttgaagac catgaatcaa
    29761 aaagaaaaag caacgatact gttagtcacc catgatccat atacagcaag ctactgcgat
    29821 agaattttat ttattaaaga tggtgtgatt ttttctgaag ttgtcagaag agggacaaga
    29881 agagagttct ttgagaaagt aatagacatg caggcgacga ttggcggagg aggaaaaatg
    29941 aatgctgtat aaactagccg ttaaaagttt ttttcggcag tcacgaggct atcttgttta
    30001 ttttttcagt ttgactttat ccgtaatgat ctattattcc ttcagcgcaa tgacctacga
    30061 tcaatcttta gtcagaagag caagtcaaga tacttcgata gatggtggat tgagttttgg
    30121 tggatttatc attactgttg ttttgctttt tttcgtattc agtgccaacc gatttttctt
    30181 gaatagaagg caaaaggaaa taggcatcta tcagcttttt ggtatgaata aactgcagat
    30241 atcaggtata tatgtgttag aaatcatgat aattggattg ttcgcctgta tttctgggat
    30301 attactcggt attatttttt ccaaactctt ttcaatgatt ttggttcgaa tgatggatat
    30361 ggatcttact agtccatttt ttatctccat tccatcagta gtagatactt tatttgcttt
    30421 tttcatcatc cttttagcag tatctgtata ttcaatatgg aaaatatggc gctatccaat
    30481 gattcgaagt tttggagaaa aagaacaagc agatagttca acaatgcgct tcaggacaag
    30541 gcaccgatta ctcgcagtca tcgggttact acttttaacg agcggatatt ttggagctag
    30601 tcattttagg gaaatcactt catggatgat cagttatcat tatcttgata cattagtttt
    30661 cttttttcct tttttaattt ttttcgtttg tgtggtcggt acttatttgt ttttctgtta
    30721 tacatttcgg ttatttttga tcatattgag taaatggaag atttattact atggtatcaa
    30781 ttttttgata atgggtaaca cgcaagttca tttgctcaaa agttggcgta caaattctct
    30841 gattacagtc attctcggaa tttctttaat gacaattggc ggcacggcta gtatctctac
    30901 tattttatcc taccaggaga aagtttcctc cccgatggac tatcagtttg atgttgagac
    30961 agagaaaaaa atcaagccga ttctagcaga ggaagggcag aagataacta aagaaatcac
    31021 cttgcattat aaagtcattg ggagtatcta tagtcaaaat ctcgaagaac ctatgtttca
    31081 aactgctaat ctgatcacgg aaacacaata tcaagccttc agaaaagtta atccagattt
    31141 gccgaacatt tctttgaaat cacctggtca tactgttttg cttgatcaaa tgcaaactct
    31201 ttttcggaat atttcattat atggatctag tattggactg ccagataatc aagaattgaa
    31261 gattcaacaa atgcttccaa gtgtattagg tgacgaaaca atggtatatc taagtcctgt
    31321 actgattgta gatgataccg tttttgaaaa agcaaaaggt gtggattatc aagtagtcaa
    31381 tgtagatgta tcaggtggaa ataatgaaaa aatagatgaa cgactgagca atgacctgtc
    31441 gataaagtgg ctcaatgctg tttattattc ttatgatatc gtaaatggaa gtctacaagg
    31501 agaaattagc acaaagaaac ttccggatga ccaaatgaag gaagcacaat ttagccaaga
    31561 aagctctcgt ttgaatttct cttccagcta ttcgaaattg cgtgcagtta atcggcgaat
    31621 cggtatcaat atttttgtca cactatttgt cgggatgatc gtgattattg ctactggtag
    31681 tattttgaca gtccggcaat tagcagaagc agaggaagaa aaagaaaatt atcacctatt
    31741 gactaagctg gggattccac aaagaaggat cagacggatg atcttcgagc aaaacgcact
    31801 taccttcttt ccacccatga tattagggat cactcacgca gtatttgcaa tcaatgtctt
    31861 cactcagtat ataaaaactg ccgattactg gttggcttat cttgtgagtg gcttattgat
    31921 ccttatctat cttttgcttt atgtcattac gagtcagtta tattgtcgga ttatcgagga
    31981 atgatatacg gaataagaca agataataaa tagaactaaa ttttgaatac catttatttg
    32041 gaataagagg ttgtgacacg atttttgtca caacctcttt attttttata ttttttaaaa
    32101 gtacgagaaa ttataaagaa aaaagcagtg atttgatgtg ctatttttat aaaagctatg
    32161 tgacaaaact gttagtaaaa aaacggtttc tgtaagctag acacatgttt ctttttgtca
    32221 aaattagtta cactttgtgt gaggttatat gaggagggaa tccaatgaac aaatggcgtt
    32281 ctaatccaaa attgaattat tggggaaaat tcatcattcg aacaattttt tatagtgcag
    32341 tactgctgtt cctgatttat ctgtatcact ataaaaatat tcaaggagga acttttatct
    32401 ataacgaatt ttaggaggaa gagtatggaa atcaaaacga ttattgaagc aatcgatgat
    32461 tggggaataa atgaacctga tcgagttgct tatcaggcgg atgagacgca tacttttggt
    32521 gaattgaaac aagcatcaga cgcacttgct tattatttag aaaaaaaggt agaaggcgat
    32581 ggaccaattg tcgtttttgg caatttggag tttgaaatga ttgtctcatt tctaggggtt
    32641 gtcaaatctg gccatgccta tttgccgatt gaagagcata cacccaagga gcgtatccta
    32701 tcgatctttc gggtggctaa accatctatg gtcatcagta tcggggactg gatagaagat
    32761 attccttcgg ttcctgtcat aacaaaaaaa gaatttcaag aaatcaagaa gataccagta
    32821 ggattttatc ccagaaatag cgtaaaaggt acagaaaatt tttatattat ttttacttct
    32881 ggtactactg gagaaccaaa aggcgtacaa atcagtcatg ataatctagt cagctttgtt
    32941 caatggacga tggaagactt tgggatcaag cctggtatgc atttcttagc ccaagcgcca
    33001 ttttcttttg atttatcggt attttctatt tatcctgcat tagtttctgg cggaatgctg
    33061 aagccgttaa ataaagcagt tgttcaagac tttcgtcaat tattcgcgac attgccagcc
    33121 ttgaagttga atgtgtgggt ttctactcca tcatttatgg atatttgctt gatggaacct
    33181 acattcaatg gagaaaatgt gccggaattg gacatgtttt tattttgcgg agaagaattg
    33241 acgaaaaaaa ctgcagaaag tctacttgat cgattcccaa atgcacggat atataatact
    33301 tacggtccaa cagaagcaac tgtagctatt tcaagtattc agattgacca gcatgtacta
    33361 gatagttatg accgactacc gattggctac gtaaaaaaag acacgaaagt ttctattgtc
    33421 caagaaggta aacctgtttc aaaaggagaa acgggtgaaa tcattattgc aggaccaagc
    33481 gtatcaaaag gttacctgaa caatcccgaa aagacagctg ctgcgttctt ttgtcaaaat
    33541 gacttagcaa gttaccatac aggggatgca ggtagactag atgaatatgg gcttttattt
    33601 tatgaaggac gtatggatca gcaggtcaaa ttacatggat accggattga actcggggat
    33661 atcgaacatt atctgctcca agacaaccga atcaaacaag ctgtcgtcgt accaaaatac
    33721 caaggaacaa aagtacagca gctggttgct tttgtcgtat tagaaaacaa aacgaaacag
    33781 ccggattttc agttgactaa atctatcaaa gaacaattac tagaagttgt aatggattat
    33841 atgattcctc agaagttcca ttatgtcgat ctgctgcctc agacagttaa tggaaaaatc
    33901 gatcgaaaaa aattaacagc tgaggtgaat cctgaatgat gttacctcat atgatccctt
    33961 atgcagagcc gttttatttt tttcttttag ccattgcgct tgtaccgatc gttctatctt
    34021 tattgatatg ggaaaaaaga ttaccagttt atcaatcagc agtgacactt ttctttttat
    34081 ttgtcagctt tggaggtaat tattggcaac agggagcggc gctgattggg tatgttttgt
    34141 ggcagacgat acttgtatgg ggctattttg tttatcggca aaggaaaaat cagacaggat
    34201 ggttttttgc cgccgttatt ttagcgataa ttccactgtt tttaacgaaa gtgactcctt
    34261 tttttgaaca tggaaaagca tcgttacttg gttttttagg aatctcttat ttaaccttta
    34321 aatctgtaca aatgatcatg gaaataagag atggcatgat caaaacatat aagccatttc
    34381 gttatatcca gtttctgctg ttctttccaa caatctcttc tggaccaatc gatcgctata
    34441 ggagatttga aaaagatcag ctccatccgc ctgttcgtga taaatatgtt gatcttttgg
    34501 gaaaaggcgt ccaaaatatt tttgtcggtt ttttatataa attcattatc ggttattatt
    34561 ttggtcaagt actgcttcct gtagtgggac gcattgcctt acagcatggt ggtatttcat
    34621 gggcattagt aggttatatg tacgtgtaca gtatgtattt gttttttgat tttgctggct
    34681 atagcttatt tgcagtcggt acaagctaca tgatgggata tcagacacca atcaatttca
    34741 ataaaccttt tttatcatgg aatatcaaag aattctggaa tcgttggcat atgacgctgt
    34801 ccttttggtt ccgtgattat gtatatatgc gattgatgtt cttcatgatg aagaaaaaat
    34861 tttttaaaag caagatcgta atgtcaaata tcggttattt tgctttgttt cttctaatgg
    34921 gtatatggca tggattgacg tggtactaca tcgtatatgg actgtatcat gcttgtctga
    34981 tctgtctgac agatgcatgg atccgattta agaagaagca taaagagcag ttgccttcta
    35041 acaaatttac gcatggctta gccgtatttt tgacatttca agcagtatgt tttagcttcc
    35101 tgattttttc aggattttta gataaacttt tttttagcta acgaatagtg acatattaat
    35161 ttatataaca aaggagaaaa ggaaaatgga agaacaaatt ttatcaatct tagaagaaat
    35221 aacaggaaca gatgaagtaa gaaaaaatcg tgatattgat ctatttgatg aagggttgct
    35281 tgattcttta gcatctgttc aattattagt cgagctagac ggagagctgg atatccaagt
    35341 ccctgtatct gaattcgagc gagaggattg gagcacaccg aataaaatca ttcaacaagc
    35401 aactgcattg aagggttgag tatgaaaaag aaactttttg ctattttcgg cccggttgtt
    35461 ttagctgttg gcttactgtt tgtgtttttt acctcctcct atcaagtaga tatctctaat
    35521 ccagaaacga ttagacgtgc ctcttcttcc atgtcagtga acgtgttgaa aggagatgcg
    35581 ataaaaaatt atgcattatc tgaaaaacaa tatattcctt tttttggctc aagtgaatta
    35641 agcagaatca gtccatttca tccgtctgtt ctggcagaaa aataccagcg aaactatcgt
    35701 ccatttttgc tgggagcacc cgggacacaa tcgttaagcc aatatatgat gatgcgttct
    35761 gcaggagatg ctatgaaaaa caagaaagtc gtctttatta tttctcctca gtggttcgtt
    35821 aaaaatggtg taaaaactga ttactttaat acgtattatt ctgaacttca aacatacgac
    35881 tggttgtttt caatgaagaa agtaacgcct gctgatcgct atttagctag aagattatta
    35941 actttctcaa aagtaaaaga gaatgatacg ttgacggcga ttctgcaaac gatcaaaaaa
    36001 gggaagctac ctttacctga atctttgaat cagctgcgca gccaatggaa catgctgaag
    36061 agagaggatg aagtattcag caatattggg ttgaaagatc gacagcaaaa aatcgatcat
    36121 gagtcaaaac gattaccaaa acaataccaa gaaacagaat tatccatttt agcaaatcaa
    36181 attggagaac gagaaacgac gaataatcca ttcgggttga aaaacgattt ttatacgcat
    36241 cgtatccgag cccacgagcc agagctcaaa cagtcacaaa aaaattggga ttatcgtttt
    36301 tcgccagaat tttctgattt tcagctggtc ttggatcaat tagcaaaaaa ccataacgaa
    36361 gtattgttca tcatcccacc ggttaacgag aaatggagcg attataccgg actatctcaa
    36421 gaaatgttac aaggatttgc caaaaagatc aaatttcaat taaatagcca aggattcaat
    36481 cgaatagctg attttgtcaa tcaagcgggt accaactatt ttatggaaga cacgatccat
    36541 ctaggctgga aaggctggtt ggctgcagat caacagatca gaccgttttt agaagaaaat
    36601 catattactg ctagtaagta tcatcttgac gatgcttttt ttagcaaatc ttggcaacat
    36661 caaataccag ataaactaca attaaaataa agaaaacagg cgagtgttgc tcgcttgttt
    36721 tctttatttt tgaatattct gaataaaaga taaaaatacc aagtttgcct gaacaaaaat
    36781 cataagcaat ggtataatca caatatgaat atagaaatgg gggaaaacat ttggatacaa
    36841 aagaagaaca gctacttatt gatttaactt ctgcaaaagg agtacctgga aatgaagaag
    36901 aggtaagaga agtattcaga gaatatgcca aaccattcgc tgatgatatt ttttatgacg
    36961 gtttaggcag tgtgattgcc aaacatggtg ctggcggagg ccccaaggtc tttatttctg
    37021 gacatatgga cgaagttggt tttatggtaa caaaaataac cgaaaaagga ttcttggaat
    37081 tccaaactct tggaggttgg tgggggcaag tcatgcttgc tcagcaagtt gaaatcaaaa
    37141 cacgtgaagg gaaaatcgtc cacggtgtga ttggttccaa accaccacat gttttaacac
    37201 ctcaagtaag aaataaacct tatgaaataa aggatatgtt cattgatatt ggcgcatcaa
    37261 gccaagaaga agccaaagag tggggcatac gtcctggaga tatggtcact ccatatattg
    37321 aatacaaacg aatgaatggc agtaaatatt tactggcaaa agcatgggat aatcgtatcg
    37381 ggacagctgt ttctctgcga gtattagaga atctatctaa agaagcacac ccaaatgttt
    37441 tattcgcagg aagcgatgtg caagaagaag taggcttgcg aggtgctcga acaagtacac
    37501 accttgtaaa tcccgatatt gcttttgctt tagatactgg tacagcagga gacactccag
    37561 gaatgacacc aaaagaagca gattcgattt taggaaaagg cccacagatt ttgatttttg
    37621 atgcatctat gatcccgcat aaaaaattat tgaattttgt tattgatgta gcagaggaaa
    37681 tggatattcc attccaatat acagtgattt ctggcggagg aactgatgca ggacaaatgc
    37741 atttgacaag agatggtgtt ccgtctgtag ccattaccgt tcctgttcgt tatctgcatt
    37801 cccatacatc agtgattcat gaagatgatt atttgaatac agtgaaacta gtcacagaat
    37861 tagtgaaacg gcttgatagt gaaacagttg aaaaaattcg gagttatgaa taatgaataa
    37921 tagaatacct atttctgaat atcggaaaaa agcacgagtt tctttggaaa atcaatgggg
    37981 aatcaatact tggcttgttt tcttatctat aatatttacg agcgtcatcc agaacttttt
    38041 tgacggtttc agtccttttc cggaagaaag catgcagcag cacttattga gttttctagt
    38101 cgataattta cttttattcg cattcaacta tgcattgttt tacattgcgc tagtagttgt
    38161 acggggtggc agaccaaaaa gtaacctttt attttcagtt ttccaaagaa agtattttgg
    38221 ttcgatactt ggcatcaatt tgatcaattc agtcatcaac tggctactca acagtttaat
    38281 ctttctgccc atgtttttaa tcagcggagt gagtacttat atgcagctgc ttttcagttc
    38341 aaatgcggta cctacgggtg tcatgccaga agattttctt gatatcagct ttgttttatc
    38401 gatgataggg atgatttttg tttcagcact catcatgagt attgttggcg gtttattcca
    38461 atttgctgca tggacaaagt tcgactatcc tgatttgtcg gttattcaat gtttgaaata
    38521 tggctggttt ttattgaaag atagaattgg tacgtatatt ttgttgcagc tgtcttttat
    38581 cggctggtat ttgctaggag caatcgcttt gttcatcggt cttttgtggg tcattgttta
    38641 tgtgaatgtg tcagttgcag aattttatga acaagcacgg attgaaaaag gaggtccaga
    38701 cgcctatttt acttagcatg taagcagttt attcatcaaa aaaataactt ttgtggtagg
    38761 ataaatcttt gagggggaat gtacgatgaa attaactgtt ttaggatgct taggagcgta
    38821 tccttataaa aatcaaggga caacaggtta tttgcttcaa tctgacgggt tcaatttgtt
    38881 gatcgatgca ggtagtgcta cgctgatcaa attacaagaa caccttgatc cacttgatct
    38941 agatgctgtg atccttagcc attatcatca tgatcatatt gctgaccttg gtgtattaca
    39001 gtattactgg caacttcatc ctaagagaaa tccggagaca ttgctttcaa tttatggaca
    39061 cacaaaagat tccattcact ttgaagaatt gacgttggaa aatgtctcaa aaggacatcc
    39121 ttattttgaa gctgaagaat taaaaattgg tccttttctg attactttta tggaaacaat
    39181 ccatccagta gtatgttacg ccatgcgtat cgtagaagaa acaacgggga aagtattcgt
    39241 atttacaggt gattcaggtt atttggaaag tttcgctgat tttgcgaaag atgctgattt
    39301 gtttctagca gatacctatc tttttgaagg aaatgaacat catcatgccc atttcacttc
    39361 aaaagaagct ggagaacttg cgaaatcagc taacgtaaag aaacttgtac ttacacacct
    39421 acctcagttt ggtgatttag aacaattgaa aaatgaagcg caaaaggcag caggcaaagg
    39481 gattgatata gaacttgctg cagttgacaa agtgtttaac atttaggagg aagaacatga
    39541 tttttgtacc aaatgaaaac cacgacccaa gaatcaattt agcaattgaa caatatttgc
    39601 tgcaagaaat gcctttagat gaacctattt tgctctttta tatcaatgaa ccttcaatca
    39661 tcatcggcag aaaccaaaat acgcttgaag aaatcaatcg agattatgtg gaagaaaatg
    39721 gcattcatgt tgtccgtcgc ttaagtggag gcggagctgt ctaccatgat tttggaaacc
    39781 tgaactttag ttttatcatg cctgatgatg gggattcatt ccgtgacttt gcgaaagtaa
    39841 cacagccaat catccaagca cttcatgaat tgggtgttgc tggggcggaa ttgaaaggcc
    39901 gaaatgatct agtcattgat ggcatgaaat tttctggcaa cgcgatgtat gctaccaacg
    39961 gtcggatgtt tgctcatgga acaattatgt tcgactctga tatcaatgaa gtagtcaatg
    40021 cattaaaagt aaaaaaagac aaaatcgaat caaaaggaat caaatcgatc cgttctcgtg
    40081 taacgaatat caaaccattt cttccagaag aaaagcaaaa aatgacaaca gaagaatttc
    40141 gtcaagaaat cttgttgaaa attttcggcg tagagtcagt agaagaagta aaaacgtacg
    40201 aattgactga tgaagattgg aaaaaaatca atcgaatctc tgaggaatat tatcgaaatt
    40261 gggattggaa ttacgggaag tctccagatt tcaatttctc tcgccaaaaa cgtttttcta
    40321 tcggttcgat tgaagtccat ttgaatgtct cagaaggaat gatcaaagaa gcaaaaatct
    40381 ttggcgattt cttcggacta ggagatatct ctgacgtgga aaaaaaattg gttggacaga
    40441 aatatgataa agcatctcta tcaaaaatag tcagccaaat tgatgtgaag aaatattttg
    40501 gtgcgataga accagaagaa ttgcttacct tactttattg aaaataaaaa atagcggaat
    40561 ccacatatat ggattccgct att
//
LOCUS       DACDIE010000019        40370 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.019, whole genome shotgun sequence.
ACCESSION   DACDIE010000019 DACDIE010000000
VERSION     DACDIE010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40370
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.019"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(162..2159)
                     /gene="tkt"
                     /locus_tag="IV250_001113"
     CDS             complement(162..2159)
                     /gene="tkt"
                     /locus_tag="IV250_001113"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAP8768412.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2261..2512)
                     /locus_tag="IV250_001114"
     CDS             complement(2261..2512)
                     /locus_tag="IV250_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HAP8768413.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2659..3282
                     /gene="lexA"
                     /locus_tag="IV250_001115"
     CDS             2659..3282
                     /gene="lexA"
                     /locus_tag="IV250_001115"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HAP8768414.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3462..3680
                     /locus_tag="IV250_001116"
     CDS             3462..3680
                     /locus_tag="IV250_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8768415.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3969..4481)
                     /locus_tag="IV250_001117"
     CDS             complement(3969..4481)
                     /locus_tag="IV250_001117"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAP8768416.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4495..6792)
                     /gene="recJ"
                     /locus_tag="IV250_001118"
     CDS             complement(4495..6792)
                     /gene="recJ"
                     /locus_tag="IV250_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAP8768417.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6934..7368)
                     /locus_tag="IV250_001119"
     CDS             complement(6934..7368)
                     /locus_tag="IV250_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAP8768418.1"
                     /translation="MKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPLILV
                     IIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQREFRN
                     ERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7378..8166)
                     /locus_tag="IV250_001120"
     CDS             complement(7378..8166)
                     /locus_tag="IV250_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8768419.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8163..9104)
                     /locus_tag="IV250_001121"
     CDS             complement(8163..9104)
                     /locus_tag="IV250_001121"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAP8768420.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9281..9868)
                     /locus_tag="IV250_001122"
     CDS             complement(9281..9868)
                     /locus_tag="IV250_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HAP8768421.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10089..11402)
                     /gene="obgE"
                     /locus_tag="IV250_001123"
     CDS             complement(10089..11402)
                     /gene="obgE"
                     /locus_tag="IV250_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAP8768422.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11690..12700
                     /gene="gap"
                     /locus_tag="IV250_001124"
     CDS             11690..12700
                     /gene="gap"
                     /locus_tag="IV250_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP8768423.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12785..13720)
                     /locus_tag="IV250_001125"
     CDS             complement(12785..13720)
                     /locus_tag="IV250_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8768424.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
     gene            13906..14865
                     /locus_tag="IV250_001126"
     CDS             13906..14865
                     /locus_tag="IV250_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HAP8768425.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERSVS
                     CGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFNSEF
                     GHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFK
                     SITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNGLPKSMDFRK
                     VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(14951..16045)
                     /locus_tag="IV250_001127"
     CDS             complement(14951..16045)
                     /locus_tag="IV250_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP8768426.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            16234..16995
                     /locus_tag="IV250_001128"
     CDS             16234..16995
                     /locus_tag="IV250_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP8768427.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(17110..17550)
                     /gene="fabZ"
                     /locus_tag="IV250_001129"
     CDS             complement(17110..17550)
                     /gene="fabZ"
                     /locus_tag="IV250_001129"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP8768428.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            17770..18525
                     /gene="fabI"
                     /locus_tag="IV250_001130"
     CDS             17770..18525
                     /gene="fabI"
                     /locus_tag="IV250_001130"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAP8768429.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(18635..19648)
                     /locus_tag="IV250_001131"
     CDS             complement(18635..19648)
                     /locus_tag="IV250_001131"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HAP8768430.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            19813..20733
                     /locus_tag="IV250_001132"
     CDS             19813..20733
                     /locus_tag="IV250_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAP8768431.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            complement(21013..21513)
                     /locus_tag="IV250_001133"
     CDS             complement(21013..21513)
                     /locus_tag="IV250_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP8768432.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(21620..22138)
                     /locus_tag="IV250_001134"
     CDS             complement(21620..22138)
                     /locus_tag="IV250_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAP8768433.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            complement(22139..23494)
                     /gene="rph"
                     /locus_tag="IV250_001135"
     CDS             complement(22139..23494)
                     /gene="rph"
                     /locus_tag="IV250_001135"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAP8768434.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            complement(23497..24318)
                     /gene="racE"
                     /locus_tag="IV250_001136"
     CDS             complement(23497..24318)
                     /gene="racE"
                     /locus_tag="IV250_001136"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAP8768435.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            complement(24480..25136)
                     /locus_tag="IV250_001137"
     CDS             complement(24480..25136)
                     /locus_tag="IV250_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP8768436.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            complement(25152..25799)
                     /locus_tag="IV250_001138"
     CDS             complement(25152..25799)
                     /locus_tag="IV250_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP8768437.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            complement(25812..26627)
                     /locus_tag="IV250_001139"
     CDS             complement(25812..26627)
                     /locus_tag="IV250_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP8768438.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            complement(26641..27378)
                     /locus_tag="IV250_001140"
     CDS             complement(26641..27378)
                     /locus_tag="IV250_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP8768439.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            27632..28642
                     /gene="trpS"
                     /locus_tag="IV250_001141"
     CDS             27632..28642
                     /gene="trpS"
                     /locus_tag="IV250_001141"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAP8768440.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            complement(28812..31232)
                     /locus_tag="IV250_001142"
     CDS             complement(28812..31232)
                     /locus_tag="IV250_001142"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAP8768441.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(31237..32280)
                     /gene="pheS"
                     /locus_tag="IV250_001143"
     CDS             complement(31237..32280)
                     /gene="pheS"
                     /locus_tag="IV250_001143"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAP8768442.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIVRKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            complement(32589..32930)
                     /locus_tag="IV250_001144"
     CDS             complement(32589..32930)
                     /locus_tag="IV250_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8768443.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            complement(32999..36721)
                     /gene="addA"
                     /locus_tag="IV250_001145"
     CDS             complement(32999..36721)
                     /gene="addA"
                     /locus_tag="IV250_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAP8768444.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            complement(36718..40245)
                     /locus_tag="IV250_001146"
     CDS             complement(36718..40245)
                     /locus_tag="IV250_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP8768445.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
BASE COUNT        11437 a         8670 c         7045 g        13218 t
ORIGIN      
        1 ctcaagaaac acaaactaat ccgaagaatt ctttgttttg tcatttaaat gtaacatagc
       61 cgaaaacgaa aagtaatatt gatttattct tctaacacat aaagaacagc tgtctccaac
      121 ttgcagacag ctgttcttat cagtcaaaca attgcttgga tctatgatag ttgattgtac
      181 gtattcacga cattttctac tgtaaatcca aattcttcta aaattttagt gccaggggca
      241 gatgcaccga aatgatcgat tgtaatcgtt gcgccctctg ttcctacgta gcgttcccat
      301 ccaaatgatg cagcagcttc gatagccaca cgtttcttca ctgatttagg aagtacactt
      361 tctttatatt cagaagattg tttttcaaag aggtcaaagc ttggcataga aacaacagaa
      421 acatctttgc ctttttcagc caattctttt tgagctttca cagccaaatc aacttcagaa
      481 cctgttgcaa tcaaaatacc ttctggtgtt tctccttgtg atggagaaag aacatacgcg
      541 ccttttttca ccatttcatc tgctacttct tttgtagaag gcaatactgg aaggttttga
      601 cgactcaata caaggatagt aggcgcatct gttgtctcca tcgctacttt ccaagcagca
      661 cgtgtttcat ttccgtctgc cggacgaatc acttgaacac caggcatgca gcgaacagat
      721 gctagttgtt caatcggttc gtgcgttggt ccgtcttctc cgacagcaac cgagtcatgt
      781 gttaagacgt aaacaacagg agtatgttgg attgctgcta aacgaactgc tggtcttaga
      841 taatcgacaa agacaaagaa tgttccaccg tagatacgag ttccgccatg aagttggata
      901 ccattcatag ccgaagccat tgcaaattcg cggacaccga accagatgtt gcgtccttca
      961 taatgttcag gagtgaaatc tttatctgca gccaccattg tattattgga acctgacaag
     1021 tccgctgaac caccccagaa gcttggtata gctttcgata attcttggat gacttcttta
     1081 cttgaaacac ggcttgcttg actgcttcca acttcataag ttggcaattc tgcatcccag
     1141 ttttctggta gtttgccatc aaaagcatct tcaaattgtt gtgctaattc aggataagct
     1201 tttttataat tggcaaacat ttcattccat gcatcttcag ctttttgtcc ttcttctatc
     1261 atcgtttgat ggaagcgggc agctacttct tctggaacag tgaaatctgg atactcccaa
     1321 ccataaactt ctttagccat cttgatgcca tcttcaccaa gaggagcacc atgaacagca
     1381 gatgttcctt ctttaggagc accatagccg atgactgttt ttacttcgat caatgttggt
     1441 ttgtctgttt cagcttttgc ttcttcaatc gctttagaga ttgcttctag gtcatttccg
     1501 tctttcacta agatatgttg ccagccatat gcttcatagc gagcaccaac gttttctgtg
     1561 aaagcttttg aagttggacc gtctagtgaa atatcgtttg aatcgtataa aacgatcaat
     1621 ttccctagtt tcatatgacc tgccatcgaa cttgcttctt gagatacccc ttccatcaag
     1681 tctccgtcac cgcatattgc atacgtatag tgatccatca cattaaagtt ttctttgtta
     1741 tacgttgctg caaggtgcgc ttctgccatt gccataccga cagccattgc aatcccttgt
     1801 cctaatggcc ctgtagtggc ttctacacca tctgtatgat gtacttcagg atgtcctggt
     1861 gttttagaat cccattgacg gaattgtttc aaatcatcga ttgtcacttg atagccagct
     1921 aaatgaagca agctatacaa cattgctgac ccatgtcctg ccgaaagaac gaaacggtcg
     1981 cggtcagccc agttttttga tgtcgttgga tttactttca ggtgttttgt ccataacgca
     2041 tatgccatag gtgctgcgcc catcggcaat ccaggatgtc ctgagtttgc tttttggact
     2101 gcttcaatac ttaatgtacg aattgtgtta acgccaagtt ggtcaatttt gtcaaacaaa
     2161 ataacccctc ctattaattt aggttttttt tacctttatt tcttacactt tcattctata
     2221 agatgtcaca cgaaaaagca atctttttca gtatttttta ctaaaagttg tcatttcggt
     2281 tgtgtaagcc ttttttcttt tggatttctt ttaatttttc cggggtcacg tcattgccct
     2341 caggatcgac gactttcatt ccttcgatat gatgacgcat accggaacga aaagcttcca
     2401 aatactcttt gcgcaagttg atttgttctt gttcttcggc ttttgagaga ccattttcct
     2461 tctttttacg tgcaagttca ttgatccgct ctattttttc aggtgacagc atgctcagcc
     2521 ctccttttac tctccttcat attatataaa aactgaaaaa aaaacaacta gataaactaa
     2581 ataaaatacg aacattcgtt tgatttagcg ctttttatat ggtactattt atttatcaag
     2641 tgaaagaagg tgtaccttgt ggcccgacaa actgaatcaa gacaaatcga agtcttacga
     2701 tatatccatg aacaagtaac ccaaaaagga tacccgccga ctgtccgtga aatcggggaa
     2761 gctgttcagc tttcttctac ttctactgtt cacggtcatc ttgcacggct tgaaaaaaaa
     2821 gggtttatcc aaagagatcc gacgaaacct cgtgcaatcg agttgacaaa acaaggttta
     2881 gaaaagattg ggattcgtcc aacaactatt ccagtacttg gcgtcgtaac agctggagaa
     2941 cccattttag cagtagagga tgcttcagac ttctttccaa tccctcctaa tctttcttcc
     3001 gaagaaggca gtctctttat gctgacaatt cgtggagata gtatgatcaa tgctgggata
     3061 ttggatggtg acaacgtgat cgttcggaag caagaaactg ctcaaaatgg cgatattgtc
     3121 attgcaatga ctgccgagga cgaagcgact tgcaaacgat tctataaaga aaaaaacttt
     3181 ttccgtctgc agcctgaaaa cgacttgctc gaaccaatca tcttagatca agtatctata
     3241 ttaggacgag tagtaggact ttatcgtagt catatctatt aattgctact aattaaaaga
     3301 aaacttttta aaaagagttt gagacgttgg tctcaaactc ttttttcgcc cgggttcttc
     3361 ttctttcaaa tttttatcac ttccctcttt acaaaccgaa caaatattcg tatactaaat
     3421 atacgaacaa ttgttcgcat aaattaaaga ggtgagaatt catgaaatca atgagacgtc
     3481 ttggcttagt attaactgtt ttgtttttag gtattattat tggtaatttc ggcatacgtc
     3541 cagctttact tgtgtttgtt ccgttattta ctttgtggtt catgttgtgg gatgaaaaga
     3601 agtaccgtca atctgagcgg cgcagaattc atgaagatca tttctatcat gagccctact
     3661 acagatccag gcaatcataa taaggattga ctgctaatta ttcaaaagac aagaagttaa
     3721 acttttacgg gggtgtgaca aaaatcggtc acatcctttt tttcgaataa acggtgttct
     3781 aaagccagtt tttcggtatt aagtcaaatt gcggaaaaag tgatttttta aacttctact
     3841 ggtctttgct tgtttccatt tataaaaaaa tagcgaccct caaaagtcag atagagaatc
     3901 tgacttttga gggtcgcttt aaaatcgctt ctttaagaat aacatcaaga atagcttctt
     3961 ttttctgctt aatattccat taatgtaatg atgtcatatc ctttgatttt gtcgcgtcca
     4021 tggagatcca tcaattcgat caaaaatgcg cagcctacga ctacaccgcc taattgttcg
     4081 atcagttcga tcgtcgcttt gattgttcct ccagtagcta gcaaatcgtc acaaatcagc
     4141 acacgttgac ctggtttaat ggcatcctta tgcaatgtca atgtatctgt tccatattct
     4201 aatccataag tcacttcgat ggtttctcga ggtaacttcc cttttttgcg gactggggca
     4261 aaaccgactc ctaactcata agcaacagga catcctacga tgaatccacg ggcttctggc
     4321 ccaactacca tatcaattcg tttttctttt gcataattta cgatttgttt agtagcttcc
     4381 cgataagcgt caccatctgc catcagtggg gaaatatctc gaaacacaat tccttttgac
     4441 ggatagtcgg gaatactggc aatataatct tttaaatcca ctctttgttt cctcctaggt
     4501 tgttaaccat ttctttatgg taggaatatc gctcaaaagc aaaaattcct caactttgat
     4561 tttattgagc cgtcgctggt ataatctgct ctcagtcaaa ggatgacttt ctgggctagc
     4621 gactttttgt aaaacaccgt tctctattgt aacaaatttc aattcaaaaa acacctgtat
     4681 cataaaaatt aataattttt cagggatacg caaatattga gctattgctt ttattttatg
     4741 acgtatatca attcgttctt gagaatggat caatttaaac aattttgcgt actgttctct
     4801 tgtgcctacc ccattcaaat aggcctcatc aggtgaaatc cccatcagat aaacacgatt
     4861 gaatgaataa gaccgaaaaa cttctttcag atccgctaaa tcatctggac aatccataac
     4921 aactagagag tttggttttc ttttttcaat tattgccttt acagaatcta gtccttcgaa
     4981 aacaatcgca gcttcctgga taatctcagg cacgaatttc aacgtcgaag aatcaaatgc
     5041 taaataaagg ctaccttccg acgttcggct ctgttcacgg aaacgttttg cccgccaatc
     5101 aaatacttgc agaccaccca cagaaaaatc agataccatc agctgcggtt tcttacgccc
     5161 gttccattca ttgatcgaaa gctttcctac cacatctacc tgattattca gcagttcttc
     5221 ttcttgagta ccaaaaccaa aagcgaccgc atcaagctga ctcgatgcat cggaaaggac
     5281 aaatttcaga tgctgttggt ttgctccgat tcttttgata ttttctgctt ggacttgtcg
     5341 aaataaaaac tgaggcaaag gattatccgt accaaatggc gccaatagtt tcagctcatc
     5401 aattcgctct actgttactt catttggcag caatacttca tcaattctca actcaggccc
     5461 tctcatcaag tcaatttgat gatcgactat atattggttc atcttctcct gtagtatagt
     5521 gacattttca cttggcatcg ttaaaccgac tgctgcatga tgtcctccga agtaagtaaa
     5581 taaatctcgc atctggtcaa gcatttcaaa aagattcaag gcttcaatac tacgtccaga
     5641 tcctttagct gatccatctt cttttaaagt aaggacaagc gttggtttac ccgtttcatt
     5701 cattattttc ccagcaacaa tgcccaaaac gccttcatgc catcctcggt ttaccaatag
     5761 atggatatga ttctccttat tgaccatcgc taatgcttct tgtgttatct gctctacgat
     5821 tgcttttctt tcttcattga tctcattcaa tttttttgct tgaacggatg cttcctcttc
     5881 atcaaacgtc gcaagtaacg agacggcagg acgtggatcg cccatccgac cgattgcatt
     5941 tagtctggga gcaatcgaaa aaccgatcgt tgtttcgtct gcgtccctca tttttacgcc
     6001 gctttcttca aatagcgcct gaagtcctat ccgttctgaa tgatgaatgg catcgattcc
     6061 taatgaaaca aggattcgat tctcatcagt caatgaaact aaatcggcga tcgttcctat
     6121 cgctactaaa tccaaaaatt ctgcaggcgg ttcttccaat aatgcagaag ctactttgaa
     6181 tgctactccg actcctgcca aatctggaaa tggatagtgc cccgctggat gacgagggtg
     6241 aacaatcgca taagcgtctg gtaaattctc aggtagttcg tgatgatctg taatgatcac
     6301 gtctacacca gcttgctgag cgtatgctac cgcatcattt cctgctaccc cgttatcaac
     6361 agtgatgatc aactggattc cttcttcgat cttttcttgg tataccgatt ggtttggtcc
     6421 atacccatgc tcaaatcgat ttggcaggaa tacttctact tctgcaccaa gtaattccag
     6481 tgtttctttc attaccgttg cactggtgat cccatctgcg tcataatcac cgtaaacgag
     6541 gattttttct ccactgatta cagcttcttg gattcggctg attgctttat ccatatcgtg
     6601 tatcagatac ggatcatgta attcttgttc ctttggatgt aaaaatcggt tgatatcctt
     6661 ttcctcatgg taacctcttt gccacagtaa cttaccgaca aatggagata atttcagttc
     6721 acggatcatt tggagaaagg attcagaagg ctctgtctcg cttaacttcc aatcaaaatt
     6781 tgcttgtctc actttctcac tcctaactta gttattatag caaaggaaat cagcaaaaaa
     6841 aagcagaaag aaagaataaa aacagatcag atccgctaga cagatgttca gcaggacctg
     6901 atcttttgta cgaccattta tgaaatatcc ctattagtca aaataattga agctttgttt
     6961 gggcgattga gttttttgtt cttccggcgc tttcaattga ctgatttgtg cggttttcgc
     7021 ttgtaactcc gcctgataag cagcttccaa ctccgcacgt tcgtttctaa actcgcgttg
     7081 ctgttcttct tttgcttctt caattttctt ttgaagttct gtttgatatt ctgacatttc
     7141 ctgagtcagc tgtttgattt gctttttttg ctgccatagt gttgccgaag cagtcaaaaa
     7201 tacaatcagt gcaccaatca gcgcagaacc gatgatcact aagatcaatg gcgcagaaaa
     7261 ggaagtgaag ccaaaactta caggaacatt tttattgttc aatacagcaa aaagaacaat
     7321 aatcaaaact aaaacaaatc caagaatcac attgctttgc tttttcaatt ccttcaccta
     7381 ctttttgtta aaaattccgc tggctaaata atcaccaatc gttggaaaca gcgtataaaa
     7441 ttttgatgca acttctaaaa taaacggttg attgatttcc cgttttggat gtctcatatt
     7501 gcggacgatc atttttgcca acttggcagg ttctaaaacg atttgtccga ctttttccaa
     7561 atagcttccg cttggatcag ccttatcgaa gaaatttgtc tcgattggac ctggattcac
     7621 tgtcgtaaca gctacgccaa gtggtttcaa ttctaatcgc aaagcgttgg agaagccaag
     7681 gactgcaaat ttcgtggcag aataaaccgt tgattttgca gttgccattt ttccagccat
     7741 agaggcgata ttaatgatat gaccttgtct tctttctgcc atatcgatcg caaatttttg
     7801 tgtcaaaacc atcattccta agatgttgac ttcaaacata tcgtatgctt ttccaaggtc
     7861 gaatgtaagg aagtcttcga aaatcccaaa acccgcattg tttaccagaa tatcgacgcg
     7921 cccgacttct tccatcacct tttcatataa ttcgtccacg ctttcaggat tgctgacgtc
     7981 taccttgaaa gcataagcct ctttgccgct caagcgtctg cactcgtctc tgacaccttc
     8041 tacaaatgcc attcttcgtg cgcaacttat aacgattgct ccttgctttg ctgcttcata
     8101 acagatttgt gcgcccagac cgcctgaact acctgttact aaaacaactt tatcttctaa
     8161 attcatgcct cttcctcctc gcgaaatgga atctcaatgc tatcaaaatc tttcataatc
     8221 gtcgtgtttt caaacacttc ttgtgcttct ttttcaagct ctaaagctgc ttttcctaaa
     8281 tatctcgcac tgatatgagt aagtagcagc cgttttgcct gcgcttcttt tgcaacttca
     8341 gcagcttgat gagtcgtgga atggaaatag ttgtgcgcca ttcttgcttc atctttatta
     8401 aatgtgcttt catgtaccag aatatcgcta ttttccgcaa gcacaacgga attatgtgtt
     8461 tttctcgtat cgcctaaaat agtcacgatt cgtccttttt tatctggacc tacgaaatct
     8521 tttccattga tcgtccgccc atcttctagt tggatcgttt caccttgctt cagtttgccg
     8581 tacagtggtc cagctggaat acctaattct tttagacgat ccacctgcaa ttctccttta
     8641 tgatcatgtt caaccacacg ataaccaaaa ctgtcgatcc catgatttag tttttttgta
     8701 taaacagaaa attgctgatc agtaaaaatc ggggctgttt catttagctc aatgaatttc
     8761 agcggataac ttaaacgcgt ttgcgaaata cctaaagaga cttttatgta ttcctctatc
     8821 ccttttggcc cgtaaatctc taatggcgta tccccacctt gaaatgaccg gctgctgatg
     8881 agcccgggaa gtccaaaaat atgatcgccg tgtaaatgtg taatgaaaat tttaccgatt
     8941 tttctcggac gaatagtcgt tcgcaaaatc tgcatttgcg tgccttcgcc acagtcgaac
     9001 aaccaaactt cattgcgctc gtctaaaagt ttcaatgcta tacttgtcac atttctatgt
     9061 tttgcaggaa cacctgcacc tgttcctaaa aattgtaatt ccataatcta cctgactttc
     9121 tttaagttcc tctttaattt tagggtaaaa agaagggata atcaaccgta agtcgagaat
     9181 gggttcaaat taaaaataac gttctaaagc gatagcgttt tctttgaaaa ttactataaa
     9241 aacagcagcg aacccgtttg agatcctctg ctgtttttta ttaatttgaa gatccagaac
     9301 tttcattgtc gttggaagtt tgcggatgtt tgtccttcca agtcttttgt gcagcttctt
     9361 tcgcatgatt ttcagcttct ttcgcatcat gcacatcttt ttcgacatct tcataatcca
     9421 agcgagtaat ttctccgcct aattccatca ttaccaattg attcagtggg cgattgtcct
     9481 cttcatcagc aatcaagatc agtcctgtag ctccttcacc gattttattt cctacaaagt
     9541 cgaacacggt ctgggcatct tttatttctt tggcatcttg tgtcgcaccg atcattcctc
     9601 cggcaaacca tcctaataaa atccctagcg gaccacctaa tacaccaacc aacattccga
     9661 ttaatccacc cgtagaagat ttgttattac cggtaaagtc gataaaatct tctattttga
     9721 attgatgtgt cccattagga tcatggctga caactgccat ctgttctcct ttaatcgaac
     9781 gaacagcatg taactttttg atttctgaaa atgcctgata tgcctggctt tctacttgaa
     9841 aatttaaaat gattactcgt ttgctcatta aaaacgcccc tttcatacct tcttgttatt
     9901 tattttacct ttaattgaaa aataagaaaa gaaaagcact ctttttttcc aataaaatag
     9961 ggctgtgaca aaagtcgtat cacagctcct ttttccgaat aaacggcgtt cttgaagctg
    10021 atcacttctg tcacaacctc ccttaaatct gaatctatat tttttggtat ttgtttttat
    10081 ttgcttgcct attcgacaaa ttcaaattca aactcaccga tacgtacaag gtcgccatct
    10141 ttcgcaccgc gtgcccgaag agcttcatcc acacccatac cacgtaattg acgagcaaaa
    10201 cgcatcactg tttcatcatg atcgaaattc gtcatttgga ataatttttc gatcttctca
    10261 ccagataaga tccacgttgc atctgaatca cgatcgattt ggaactcagg accttctgga
    10321 ttgaatccat agtgtacagt ttcttcttcc agttcttctt cgtacaatgg gaattctggc
    10381 gttacttcta acaaatctgc tgtagcattc aataacgcgt ccaatccttg tcgagtcact
    10441 ccagaaattg gaaatactgg gatgtcatca gcaaattcat cttctttttc cttgttcagc
    10501 tgttctttga atttcactag attttccggt gcatccggca tatccatttt gtttgccacg
    10561 atgatttgcg gacgctctag caagcgtaag ttataagtag ataattcttt gttgatggca
    10621 agatagtctt cataaggatc tcgtccttcc atcccactca tatcgatcac atgtaaaatt
    10681 actcgtgttc tttcgatatg acgaaggaat tgtgtaccca gcccaacacc ttgcgaagct
    10741 ccttcaatca agcctggcaa gtctgctgcg gcaaaacttc tgccatcact tgtagtaacc
    10801 attcctagat taggtacgag cgtcgtgaaa tgataagcgc cgatttttgg acgagcagat
    10861 gaaataacgg atagtaaagt cgatttcccg acagatggaa aacctacaag tccaacgtct
    10921 gccaatactt tcaattctag ttcgattttt cgctcttgtc ctggttcacc gttttcagca
    10981 atttccggtg caggattccg aggagaggca aaacggatat tccctcgtcc accgcgtcca
    11041 ccttttgcca caactagtgt ttgaccattt tcaattaaat ctcctaaaag agcaccagtc
    11101 tccgcatctc ggacagtggt tccttgtggt actttaacat aggtatcttc tgaaccacga
    11161 ccgtgcatcc ctttactcat cccattttca cctggctgcg ctttgaaatg gcgattgaac
    11221 cgaaaatcca tcaatgtacg taacccttca tcgacgatca agatgacatc gccgccacga
    11281 ccaccgtctc cgccagccgg tccgccgtca ggaacgtatt tttcgcgacg aaatgcaacc
    11341 attccgtctc cgcccttgcc agctttcaca tcgatcgtta cttgatctaa aaacatggac
    11401 ataattaagt cctcctatta ttcatttatt tatttgactg cctgaatttc cacaataaac
    11461 aaaagtctta cttattttat cggtgaaatc tgattttgtc tatcatttca gcaaaaagat
    11521 tgtttacctt cttctttttt tatcatttgg taaggtttcc aacctttttt ctgctaccaa
    11581 gtataatttt ttgacaaaat attctttgcg tagcagaata acagtgtatt aattgtatta
    11641 attgaggatg ccgtttcaca gcacccctaa taaaggagga acttttgata tgacagtaaa
    11701 agtaggtatt aacggatttg ggcgtattgg ccgtctggct ttccgccgca tcaaagaagt
    11761 ctcagatgat attgaagtag tagcaatcaa cgacttaaca agtccaacta tgttagcaca
    11821 attattgcaa tttgactcta ctcatggtac ttatcctggc acagttactg caacagaaga
    11881 ctcaatcgta gtggatggcg aagcaactcg tgtttatgca gaacccgacg caagcaaaat
    11941 cccttgggca aaagaaaacg gtgtagatat cgtactagaa tgtactggtt tttatacatc
    12001 tgaagaaaaa gcaaaagcgc atttagatgc tggtgtcaaa cgagtagtta tttctgcccc
    12061 agctggtgct atgaaaacca tcgtctataa cgtcaatgat gatacattag atgaaaacga
    12121 caaaatcatc tcagcaggtt catgtacaac aaactgttta gcacctatgg cttatttctt
    12181 aaataatgaa tttgggatcg aagttggtac aatgacaaca gttcatgcct acacttccac
    12241 acaaatgctc ttagatggac ctgtaaaagg cggtaatttg cgtgctgctc gttcagctgc
    12301 tgacaatact atcccacatt ctactggtgc tgcgaaagca atcggcttag ttatccctga
    12361 gctacaagga aaattacaag gacacgcaca acgtgttcca gtcgttgatg gttcattaac
    12421 tgaattggtt tcagtattga aaacaaaagt aactgctgat caagtaaacg aagcaatcaa
    12481 aaaacacaca gttgacaatc cttcattcgg gtatgatgac cgtcaaatcg tttctagcga
    12541 tgtcattgga acaactcaag gttcgatctt tgacccaacg caaacagaag taacaactgc
    12601 tggtgatttc caacttgtta aaactgttgc atggtatgac aatgagtatg gatttacttg
    12661 ccaaatgatt cgcttactag aaaaattcgc taacttgtaa tttgatagat caatcgataa
    12721 aagaagcctt tgactggatc cagtcaaagg cttctttctt ttcatcccct acttttaaac
    12781 tcgttcaatg aacatattgg cttaaaaatt gttggactac ttgctgatat ttttcaggat
    12841 ctgtcttgta tgcttccgca tgctcggctc cttttacaac atacttttct tttggtccct
    12901 tcgttgcacg atagacctta tctaacattt caaatggtac aaatgtatct tcgtctccat
    12961 gtataaagag cattgggcgc tggtttttct tcaattgttc cactgcattt gcttcaccga
    13021 aaaagtagcc agcacggact tttgtcacta gactcgttac ttgcataagc ggaaatgctg
    13081 gaagactaaa catatcgtct agctgataag caagctcatc atgaacggaa gaataaccgc
    13141 aatcttcaat gattgctttc acattatctg gtagtttttc tccgctagtc atcataactg
    13201 ttgctgctcc catgctgaca ccgtatagaa cgatttcttg cgattttcca ttgttttcta
    13261 gcactttgtt tatccactgt acatagtctt tacgctctgg ccatccaaat ccaatgtaat
    13321 ccccttcgct ttttccatgt cctcttgcat caggaacaag gacattgtaa cccatatcat
    13381 gaaacatctt tgcgtaattg gtcatcgttt cagcgtttcc catatagcca tgagccacaa
    13441 gagctgtttt ttcacttttc gtttcagcag gaagataaat cgctgataat ttcagaccgt
    13501 cttttgaagt gatggtccaa tcgcttcggt tttcttttga actaaaccat ttatgtgcat
    13561 ccgtttcttt ggatgtctgt gttgtaccag gtgtatcttc gtctaaaaag tctttctcag
    13621 aaggcacaac agcataatta taaaaataat tccctgcaaa cactaacgca acaatcagag
    13681 ctaaagcgac gcctaaaagg attttcatcc aaagtttcat aaatcatcca tctcatttct
    13741 cttttttcat ataaccttga attagtataa tcaaagaatc tctactatgc aatgttcatt
    13801 tttataaccc gcgaattgta aaattaagtt agaaaaataa aaaggcattt atggtacact
    13861 caaattgtat ttccgacgaa agaaaacaaa ggagtgtaac cataaatgac ctacaaacat
    13921 cttaccatag acgaactgac aatgatagaa tcatattatc ttcaacataa taagccggtt
    13981 gaaatcgcta accgaatggg acgtgctata caaactattt ataatgtagt caataagttc
    14041 aagcaaggca agactgctct tgattattgg caccagtata aagaaaataa gaaaaaatgt
    14101 ggtagaaaag tcattcaatt acctgctcat gaagtagatt acattaaaga gaaagtcact
    14161 cttggttgga cgcctgacgt cattatcggg cgaaaagaaa ggtctgtttc atgcggtatg
    14221 agaacacttt atcgtttatt ttctaaagga atatttgata ttgacacact accgatgaaa
    14281 ggtaaaagaa aacccaatgg ccatcaggaa aaacggggaa aacaacagta tcagcgctca
    14341 atccatgatc gacctgataa ttatcctgat ttcaattctg agtttggtca ccttgaaggt
    14401 gatacgatcg ttggcattca tcataaaagt gccgtcatta ctttagttga aagattatct
    14461 aaagtcatta tcacgattaa acccaacggc cgtaaggcat tagatattga aactgccctt
    14521 aatcaatggt tttctcgctt ccctaaaaac ttctttaaat ctattacgtt tgactgtgga
    14581 aaagaatttt ctaactggaa agccattagt aaccaacatg atattgatat atattttgcg
    14641 gaccctggaa caccttctca acgcccatta aacgagaatt ctaacgggat tctgcgtcgt
    14701 aatggactgc cgaaatcaat ggattttaga aaagtgaatc agacatttat ttccagtgtc
    14761 agcaatcaac gtaatcatat tccaagaaaa tcattgaatt acagaacacc aatcgaaata
    14821 tttttgagct atgtacaaga agcattttat tctagcttaa tttgacaaat cataacctat
    14881 aaaaaaacaa aataaaaaag cagaaataac tggctatttc tagcggatta tttctgcaat
    14941 aactaattat ctatttttta ttttcaatta atttaaagct tgaccatggt ttcaaaaagt
    15001 caaggaggaa taattcgtca tcggagattc ttcctacaac attgacacgt ccgtcgtttt
    15061 tcatatcctt caaggcgatt tgtgtttctc ctttgtattg accgtaccct tcattgtcaa
    15121 tcagtacatc cccatgatgc atatctcgcg tattatgagg cgggaaatct ttatctttgt
    15181 agtatacacg agtcatcgtt gaacgaagga tatattctga gcgatcccct cgataactat
    15241 gaaggttatc aaacaagcag attcgttcgt tttctgtaat accttcttcc gtatctactt
    15301 tcaatgttgg ataatccgcg ttaaatgctt ctgccatttc tttcaattct tcttcacttg
    15361 cgtacgcatt tccaatagaa atatcatcaa tcaatcctgt aaggattaaa tgcttcactt
    15421 gtgtcgcaat ttcaagatct cggtggtctt ccagcgtgca caagccatct tgcgtaggcc
    15481 aagggccaaa ctcagcagat tgcgaattga cgaatgccat cgtattcaaa ttatattttc
    15541 tgaatttttc tgaacaaaaa acaaagtgtt cgtatcctaa accagagtat cgatgaggat
    15601 aaaaattatg agaacctaat aaattatccg tgtttggcga ataactcatg atgttgtcaa
    15661 cgtaattcgt gcccgaactc atattgatct cgattttgat accaaaagga ttgcgcgtca
    15721 tttttgcttc ttctgctcct gtgaatccga tatctaagcg gaccccgtag gcacccatct
    15781 tatggaaaaa agataagtca tcgtaagaaa tctccaactg ttcaaataac gctggattga
    15841 tatcaaccat tacttccatg cctaggctat tcgcgtaatc aactacttct ttaaattctg
    15901 aaagaacttt ttctttatca tcattgatct gcagcaaact ggtaaaaacc cgtttatacc
    15961 catacttatg agctaaatcc aagtacgctt tgtccttttc aaatgttgac cgttctggat
    16021 aaatcgagat tcctaatttt cccatattac catttcctcc tgcatctttt cgtttttgta
    16081 tccacttaca ttatagcaaa cacaaagtaa atggtctaca ccttttcttt ttcttttaaa
    16141 ataaatgata aattccaata atttaagtag gtattttccc tctattttgt tatcattaaa
    16201 gataacaaaa tagtaaattg gaaaggaaca gttatgaaga aaaataaagt tattttaagt
    16261 attagtttag cagcagcatt gctgtttgct gctggttgtt ctgcaaacaa cacgacttct
    16321 tctagttcgt ctacagaaag ttcaaaaagt acatctactg cggaaagcac gactgccagt
    16381 agcgtcgatt tgaacgcatt ggaattacca caattgacga aagatgtcgc cgaaaatgaa
    16441 gatctggtcg aaatggtgac gaataaaggc acaatcaaaa tcaaattatt tcccgaacac
    16501 gcaccaaaag cggttgaaaa ctttatgaca catgcaaaag acggttacta caacggactg
    16561 actttccatc gcgtcatcaa agatttcatg atccaaggtg gcgatcctaa aggcgatggc
    16621 actggagggg aaagcatttg gggagaagga tttgaaacag agatcaacaa ccacctttat
    16681 aatataagag gtgcgttatc tatggctcgc tcacaagatc ctaacagtaa cggcagccag
    16741 ttctttatcg ttcaaaatac agatgatatg catgatggtc ttttaaaaga cgactatcct
    16801 caagcaatca tcgacgctta taaaaacggc ggttatcctt cactagatgg ccattatact
    16861 gtcttcggcc aagtgattga aggaatggat gtcgtggatg ctattgcaaa tatcgaaaca
    16921 gatagttcag acaaaccaaa agaagaagta aaaatcgata aaatcaatat actgcaagaa
    16981 gcaaaacaaa aataaaaaaa taacgtctga atcctgcata gatgacaacg tatgctagga
    17041 ttcaggcgtc ttctttttac ataaaaagca agtaagcttc cttttgtttt gctatatcag
    17101 gttttatagt caagcatttt ggttgacgat gaacgtgaac tcgcactcac agactttttt
    17161 atcatctaca tatgctgcag ataacgctgt accgactgat cccttcattt tggtgatttc
    17221 aaaatgacat ttcagcacat ctcctggaac aactttttga cggaatttgg ccttattgat
    17281 cccaccgata taagctcttt ttcctagaaa ctcttctgat tttaggataa gtatcgaacc
    17341 tgcttgtgcc agtgtttcta gaatcaacgc acccggcatc actggattgt caggaaagtg
    17401 accttggaaa aaatcttcat tgattgttac attttttgtc gcaacgatct tttttcctgg
    17461 ttctaaagta tctacataat caatataaca aataggataa cgattaggaa tcaattccat
    17521 tacttcagta gcagataata cgtaagacat aattaaactc cttctttcag gcaattgcca
    17581 ttttcattac tttgactatc aaagtatcat attcctaaat atttagcaat ctttttttaa
    17641 tttaagaaaa aatagtttga caatcaaaac attttgacta ataattaaga aatcaagaag
    17701 ttattttagc tattattctc tattttttta cgactatcaa atttttatca gaaacaaagg
    17761 agagtcatta tgtcatttct agaaggaaaa aaaatcgtca ttatgggcgt cgctaacaaa
    17821 aaaagcattg cttggggttg tgctgaagca atgttgaaac aaggggcaga aattatctat
    17881 acttaccaaa atgaacgaat gaaaaaatca ttagtcaaat tggtcggcga agaagcattc
    17941 actgtagaat gtgatgtttc tagcgacgaa agcatcgaaa aagcaatgaa tgccatcggt
    18001 gaatatgctg gggagatcca cggccttgtc catgcagttg cttacgccaa caaagaagaa
    18061 ttgtccggaa atgtgtctga tatttccaga gacggctatc aattagctca agatatcagc
    18121 agctactctt taattgctgt atcaaaatac gcacaaccgt ttcttgcacc aaacagcggg
    18181 atcgttagca tgacttatct aggttcagaa cgcgcaatcc cgaattataa catgatgggt
    18241 attgcgaaag cttctcttga agctgctatc cgttatttgg cagctgagtt ctcgcctaaa
    18301 ggaattcggg tcaatggaat ctcagctggg gcaatcaaaa cattagctgt cacaggtgtt
    18361 aaagactatc aaaaactgat ccagttatct gaatctcgta cgcctgatca gcaaggtgtg
    18421 acgatcgaag aagtcggtaa tgcttgtgct ttcttaatca gcccactttc cagcggaatc
    18481 atcggtgata ttatctacgt tgacaaaggt gtccatcttt cataaacttt tgttgcattc
    18541 tttaaagtaa aacatataaa aatcaggaaa aaacttttta aagtctcttc ctgatttttt
    18601 ctttttacgt ttttttcaac tcattacttt gaaattattc cacgacttca atccagcctt
    18661 ctggtgcttc tatgtctcca aattgaatac ctgtcaattc atcatacaac cgttttgtta
    18721 ctggtcctac ttccgtttca ctataaaata catgaagatt gttattgtga taaatcccac
    18781 cgattggaga aataacagcc gctgtaccac atgcaccagc ttctgcaaat tgatctagat
    18841 catcaatgaa aatatctcct tcaattgctt gcagtcctag tcgttcttca gctaactgca
    18901 acaaggaata tttcgtgata cttggtaaga tagagggcga ttgcggagtg atgaattttc
    18961 cgtcttttgt aattccaaag aaatttgctg cacctacttc ttcgattttc gtatgtgtgg
    19021 ctggatcaag ataaacacaa tctgagtaat ccagttcttt tgcttctgtt cctggaagta
    19081 aactcgctgc gtaatttccg ccgacttttg ctgcaccagt ccccttagga gcagcacgat
    19141 cgtaatcaga tacaataaaa ttcgttggtt ttagtccacc tttgaaatac gctcctactg
    19201 gcgtacagaa gacgacaaat aaatattctg gagctggatg gacaccaatg ttttcaccga
    19261 ccccaatcag taaagggcgc aagtataatg tagcgcctgt tccatagggc ggcacaaaat
    19321 cttcatttgc ttttaccact tgcttgacag catcaacgaa catcttttca gggaccattg
    19381 gcatcagcaa tcgtctagca ctttgattca tccgtcgact attttgttca ggtcgaaaga
    19441 gattgacctt gccgtctttt cgacgataag cttttagacc ttcaaaacat tgctgaccat
    19501 aatgaagcgc tggagagcct tcatgaatcg tcaggtaatt atcttctgtc aattctcctt
    19561 cttcccattg tccgtctttc catttggcaa taaaacgcca aggagttttg atataagaaa
    19621 aacctagatt gttccaatcg atttgcatat acttaccacc ttcgttattt tctccacatt
    19681 ttatcattat ccatattatt tgtcatttta ttttcagctt ttttccttca ttatagttat
    19741 ttttgctatt tccttttttg aaaacttctt ggtacaatac agaaagaatc aaattttaga
    19801 aaagaggatt ttatgttttc attattagca actgccggta cgacagactc ttctggcgag
    19861 acattgacag atcaagctgt cagcaaagta aatgcgtttc agcggttttg ggacgggatt
    19921 gattgggacg ccattttggc tgcctttatc caaaaaagtt tatatctgat ttttttgatc
    19981 gtcctttttt ggattcttca ccgtgtggga aaatatctga ttgataagtc ttttaatcag
    20041 tattctaaaa aagccattct aaacgagagc cgcttaaaga ctttacatag tcttttaaat
    20101 actatttttc attataccct tggtttcttt tttatctatg caattttatc tgctatcggt
    20161 gtgccgatcg gatcactttt ggctggtgcc ggaattgcag gtgtggcaat cggtcttggt
    20221 gcccaagggt tcatgagtga tgttattacc ggatttttta ttatcatgga acaacagatg
    20281 gatgttgggg actacatcaa gctagcaaac ttaacgattg aaggaaccgt cgtatctgtt
    20341 ggtatccgta cccttcagct gaaagcaacg gatggtactg tccatttcat cccgaatcga
    20401 aacatcacca cgatcagcaa tctctctcgt gcaaatatgc aagtactttt agatattcgg
    20461 attgttcctg aagaaggata tgacaaaatc tatgagatca ttgatcgagt aaatcgtaca
    20521 ttagctgaaa aataccaaga agagctccaa actgaacctt ctatctttgg attagtggat
    20581 cttggaaaca gcaattttgc gattcgtacg gtctgctacg cattgaacgg aaaacaatat
    20641 gccttaaaag aagagttctt gagctcctat gtcaaagaat tgacagcttc tggtttcacg
    20701 attccgaaca gtccaattgc tgtcggaaaa taatttggac aaacgatgca acgaatctca
    20761 ctaaaaagag cgtgggacaa aagtaaagaa cacttttgtc ccacgctcta aacacgtata
    20821 aacggcggaa gcagaagcaa ctccttcgga aataagctga aattcacaaa aatttgaaaa
    20881 gcaattttcg tgaattcctt cttatttctt ggagttaaac gcttttgtcc cagcctcttt
    20941 ttcaagataa agtcaaaact ggtacgctgt tttttctact tattttttga ttatctttta
    21001 gtttctcgtt ctttagctca cgatatcgat ttgttctttt aattcttcga ttttcggaga
    21061 caatatatta cgacgttcta attcatgcac catgaagttt acggctttaa gcagctcttt
    21121 tcttgtaatg attcctttga aaatccgatc ttctgtgaca accggtaaaa aaggcgaatc
    21181 gactaacaaa tggagtactt cttctagttc ccaattttca tcaattgtcg gaacatctgt
    21241 ttctgtcaca tcagcaaccg ttagtccttc taacgggtcc atgctgattg tttgcaagtc
    21301 aatcatttta ttgaccacat ctgataaact tactagtcct acaaagtgat catcttttcc
    21361 taatacagga attttggaat aaccgacctt cgataacaca agtaaaccat gggacaatgg
    21421 atgatgatac ataacatttg ccacattttc tgcaggaatc aaaaacgttt cctgattttc
    21481 cagcagcatt tctcttacta taggtccaat catctgatgg cctccatttc tatttcattc
    21541 aacactacta ttttaccgaa accggatgca aaattcataa aataaagctt atttttatta
    21601 aaaattagag tttttttatt cagcgctctc tcgaaaaaga aaaggccaac tcttcaactg
    21661 cctgatgggc acggttatag tattgaacag aaatttggtc atctaaactt tcgataagag
    21721 catatgatgg gatttggatc gggcctctag gctgagaaat gctaccagga ttcaaaaaca
    21781 ggattttctg atccatctca cagcctattt gatgggtatg accaaaaaac accatatcgg
    21841 cagttgcagc tcgagcttcg attgctaact gtgtcaaacc aaatcggaca ttcgataaat
    21901 gaccgtgagt catgaatact gtatcctgac cagtttggat cacttttttc tgttcgaatc
    21961 ctggtccata atcacaatta ccttgaacca cttggaatgt atcccataat gggtcagaag
    22021 cttctaattc cgaatcacca caatggaaaa agatatccac ttcagaacga taacgatcta
    22081 ctaagtcaac gaggatatct ctgtctccat gattatcact tactactaaa taccgcatct
    22141 atctctctcc ttccagccat tgtttccact cggcactcaa ttttttcatt gcttgagccc
    22201 gatgactgat cttgtttttt tcttcgctcg ataattctgc tgctgtttgt ttcttacctt
    22261 ctgggataaa taaaggatca tacccaaagc cattttcgcc agatggtata cgggctactc
    22321 gaccgggcca ttccgcttct actacaaggc tgtctttttt gggtgccgcg aatactaacg
    22381 tacaatggaa ttgtgctgtt cttttttcat ccggcacatc tgtcaattca tataataatt
    22441 tggcgttatt ccccgcatca cttttttgtt cccctgcaaa acgtgcagaa tagattcctg
    22501 gcataccgcc taaagcatcc actttcaaac ctgaatcatc tgctaagact ggctgatcaa
    22561 gcaattgtgc aatcgtttct gcttttaatc gggcattttc ttcaaatgtg ctgccagttt
    22621 cttccacatc tggcaattca ggatagtcaa gtaatgtttt tacatgatat cccgcttctt
    22681 tgaacatatt tcgaaactct tctgcttttc ctggatttct tgtcgcaatg atgattgttt
    22741 ttgtttcttc tatccgctca tcattttgag caaactctgt tagcaaagca tgtttttgct
    22801 ctttgatcaa atcttcaatc gcgttttttc caaaaaagag catttcattc aactgttcac
    22861 catcaaacgt tgcttcttct ccagttcctt gaatctccac gaattttcca gattctgtca
    22921 ttacaagatt catgtctaca agtgctgcgg aatcttcttg gtaatccaaa tctgtgacgc
    22981 aagtcccatc tggtaagatt cctacgctaa ctgcagcaag atgttctttt attggatctt
    23041 ctgacaattc tttttctctt aaaagttttt caatggctaa tttcaatgcc acaaatgccc
    23101 cagtgatact tgctgttcta gtaccgccat ccgcttggat cacatcgcag tctacgatga
    23161 tactacgttc tcctaatttt tccaagtcta ctactgcacg caaggaacga ccaataagac
    23221 gctggatttc cattgttcta ccagaaagtt tccctttgct gctttctcta cgatttctcg
    23281 tactcgttgc tcgaggcagc atactatatt ctgctgtcac ccatcctgtt cctgtttctc
    23341 tcaaaaaagg gggaacagaa ttttctattg ttgcagaaca aataactgtt gtatcaccaa
    23401 aacggatcac taccgatcct tctggatatt tgaaaacatt tgtttgaagc tccagtttgc
    23461 gaagctcttg ggcttttctc ccgtcatgac gcatccttat tttcctccca attcgatatg
    23521 ttctacattt aagtgtccga ttccaagcca gtcttcagct atttcctcga aaagtttggc
    23581 agagccagtt gtataaaact ggcataatgt ccgaccattt tgcggtgaat tgctcaaatt
    23641 gaaataatct aacagcatag atacttcacc tactgtttct gctccagaat cgatcagttg
    23701 aacattttct cccattacat tttgaatgat ggggcgtaat aatggataat gggtgcatcc
    23761 caaaatcaat gtatcgattt ttttagtggt taaaggagct aatgtttctg ccacaatttt
    23821 tttcgccacc gatgaatggt attcattact ttcgacaact gaaacaaatt tgggacaagc
    23881 aagacttgtc acagtcaatt ctggtacttt ctctttcagt gctttttcat aagcttgact
    23941 ttttaccgta ccaatcgtac cgataatgcc aacttgttta ttttgtgttt tttttactgc
    24001 cgctctagta ccgggaagga tcacaccgat gactggaata gaaagagcag ctttgatttc
    24061 ttctaaagcc accgcagttg cggtattgca ggcgatcacc agcatcttga ttccttgctc
    24121 caccagataa tccgtcattt cccaagtata ctggattacc tgttccgcgg gtctagggcc
    24181 atatgggcag cgtgctgtgt ctcctacaaa taaaatattt tcattcggta attgtttcag
    24241 ggcttctttt actacagtca agccgccgac acctgaatca ataaatccga tagggcgatt
    24301 atctgtcaat cgtatcatcc tttttttcaa taaattctat cctccctatt gtgtactttt
    24361 tagcgtgaat tttcaagtgc tcttacttgc ttaaaacaat cgaaatctct ttttttcgaa
    24421 tggaaagagg aaaagcggtt gtgactttag tttgccacaa ccgctaaaag aatcatctat
    24481 taataactgt accgtctctt ttccaagtaa ccggaaagca gagaaagcgc gtaacatacc
    24541 acgaagtaca taagtgtcat gatgatgaac atcggaagca cataattggt gttctgtcca
    24601 taaataatac gtgcctgatg agaaagttca ggtaaagaaa tgattactgc taatgaagta
    24661 tctttgatca gtgaaatcaa ttggctcaca attgccggga tcattgattt aaatgcttgc
    24721 ggtaacacga tcgttcgcat cgattctaca taagtcagtc ccgttgataa tcctgcttcc
    24781 atctgtcctt ttggaacagc attgagtcca gctcggaaaa tttccgacaa catagctgac
    24841 tcaaaaatcg ttaatgctgc gacagcggac cagaagatgt tcatctgaat gccgatttga
    24901 ggtaaggcaa agtaagtgaa gaaaatgatc aataataatg gaagattccg tataatatca
    24961 atcactacac caaggatttt ggaaaagaaa gggatattcg caaaacgaat cacacctgtc
    25021 agaccaccga tcaagaaact aaaaatgatc gaaaacaatg aaacttcaac agtaaccttt
    25081 aatccttcca ataaaaaacg gatattcatc catgaaaaag cgccgctaaa atccatgtat
    25141 ttcccctcct tctatgaacg aaccgcccat tttttctcaa gataaaccat taagtaagat
    25201 aacggtagcg tgataactaa gtaaaataac ccaacgatga tatacgtatc gaaggtattg
    25261 aatgtttcac tagcgatcaa gtctccttga tacatcaaat ctaaaccagc tacgattgct
    25321 aaaatagatg agttttttac gaggttgata aattggttgc ctaatggcgg gataacgatt
    25381 ttgaatgctt gcggtaaaac aatataacgc atgatttctg aataggtaaa accagaagat
    25441 agtcctgctt ctgtttgacc ttttggtacg gtttgaatcc ctgaacgtac tgtttcagca
    25501 ataaaagcag atgtatagat cgttaatcca atcgtacctg cttggaatcc atctagatta
    25561 aaccaataaa tcggtacgac aacataaaaa aacatcacga tgattaataa tggaatgttt
    25621 ctaaaaaatt ctacatatgc ctttgctaag gcgctcacta ctttattctt tgataattgg
    25681 aagattgcca ttaacgttcc gataatcaaa ctaaacacaa gtgctaagac actcgaaaac
    25741 aatgtgatct tgaacccgtc gacaaagatt ggtccataag tttgtatcaa ttcgctcatt
    25801 gcctctcccc cttactctac ttttccttca gtttcatttg gaaaccattt ttgatagatt
    25861 ttatcgtatg tgccatctgc atgcatttcc tctagcgctt ggttaacagc tttcaagaag
    25921 ttttcttgcc ctttgttgat cgcaatcccg taaggctcat tagtaaacgt tccaccaact
    25981 aactcatact ctggattttc atctgcaatc cctaacaaaa tcgcattatc cgtagtcatc
    26041 gcgtctccct gaccagattg taaagcagta aatgcttctg catagttttc taattctaaa
    26101 atttttgcat ctggtgcatg ctgtcggata ttcgcagccg atgtagaacc tttgactgct
    26161 agaaccgttg tagaagcatt caaatcatct acacttttga tttggcttcc tttctttacc
    26221 aacaaagctt ggccagcatc gaaatagaca tctgaaaagt caacctgttt ttttcgttca
    26281 tctgtgatcg tcatcgttgc aataattgca tcgatatttc cgtttttcaa taatggaatc
    26341 cgagttttag aagtgacttc aacaaattct gtttttccat tgtcaccaag gatcttttta
    26401 gtgattgctt ttgcaatatc tacatcaaat ccttgtacag ttctgctctc aatatccatc
    26461 attccaaata atcgagtatc atacttgaca ccccagatga tttcattggt agatttgctt
    26521 cgctctaaaa tatcttcatt tgccaaccca tttcctttac atccggctag tgcaagaaaa
    26581 gcaatgccta aaaggaaaat caggcgtatt ttttttaacc tgcgcatctt tttcctccta
    26641 tcaatgattg ataactttac tgataaattg ctgtgctcgg acttcatttg gattttcgaa
    26701 aaagtctctc acatttctgc tgtcttccaa tacttctcca tcagccatga agataacacg
    26761 atccgccact tctttggcaa agcccatttc atgtgtcacg acaatcattg acataccgtc
    26821 atgagccaat tttttcataa cgtctaatac atcaccaatc atttccggat ccaaagcaga
    26881 agtaggttca tcaaacagca gcatctcagg attcatcgct aaacctctgg cgattgccac
    26941 ccgttgctgt tgtcccccgg ataacatgga aggataagaa tctttcttat ctaacatatt
    27001 gacagttttc aaaaatttct ccgcgttttt gactgcagtt gcctggtctt gcttcaacac
    27061 tttgattggt gctaatttga tgttttccag aacagtttta tttgggtaca aattgaaatg
    27121 ttggaaaacc atgccgatgt tttttcggac ttctgtcagt tttgtctttt tatcatgcaa
    27181 gtcagtatca ttaatcagca gcttacctga agagatcgtc tctaatccat tgatacagcg
    27241 aagcatcgtg ctctttcctg aaccagaagg accaatgaca acgacaactt ctcctttctc
    27301 gaattcgaga ttgatatttt ttaatgcgtg gaacttgcca taatatttat ccacgtgatc
    27361 aaatttaatc attgccatag aattacactc ccattattat ttagtaagat ttcttttaaa
    27421 gatgtataat aaaatacatt atatctgata gtttagtaat ttgtaaagtt tcttagaacg
    27481 tttattctcc cataaatatt tcttccattt ctaatctatt agttatttta tcctctttcc
    27541 ttgttttttc cttaggaact gttgtttctt ttcaggaaaa acggtaagat attaagtgct
    27601 cagacttaca aatcgtaaag aggtgtcttt tttgaaaaat attattttaa cgggagatcg
    27661 accaactggg aagctgcatt tagggcatta cgtcggttct ctaaaaaccc gagtggctat
    27721 gcaggcagat gaagacaacc aactgtttgt catgattgct gacatgcaag cactgacaga
    27781 taatgctaaa aatcctgaga aggtttcatc aaatgtcttg caagttgctt tagactattt
    27841 agccgtggga ttagatcctg caaaatctac tttatttatc caatcacaaa ttccggaatt
    27901 agctgaatta accatgtatt atttgaactt agtcagcgta ggacgcgttc ggcgcaatcc
    27961 gactgtcaaa acagaaatcg aacaaaaaaa attcggtgaa agcgtgccta caggattctt
    28021 tatctatcct gtttctcaag cagcagatat cactgcattc aaagcgaatc ttgtacctgt
    28081 tggtgaggat caaaaaccaa tgctagagca gacacaggaa atcgtacaaa gcttcaatca
    28141 tacatatggt gaagtcttgg tggaaccaaa aggcgtattc ccaccaaaag gaatgggtag
    28201 attacctgga atcgatggaa atggcaaaat gagtaaatcg ttaggaaacg gtatttatat
    28261 ctccgatcct gccgatgtct tacagaaaaa agtcatgagt atgtatactg atccaaacca
    28321 catccatgta caagatcctg gacaagtaga agggaatatg gtcttcacct acttggatgt
    28381 attcggaaca gataaagaag caattgaaga aatgaaagct cattaccgcc gcggcggact
    28441 tggtgatgta aagatcaaac gatacctcat tgatgtattg gaagctgaat ttgcaccgat
    28501 ccgtgcgcgc cgagaagaat tggaaaaaga tccttcagct gttatggaaa tcttacgtaa
    28561 aggtagtgaa gaagctgcta aagtagctgc acaaacactt tctgaagtaa aatcagcaat
    28621 gggaatcaat tacttccatt aattcattta ctctaaaaaa gcaggctgta aaggcctgtt
    28681 tttttatctc gtctctgtgc tattcctttt ttaaaatcaa aaaaagagat gtgacgaagt
    28741 tgtgtcacat ctttttttca aatgtatggt cttcaaaagc tacttgatcg ttacttacta
    28801 tattttcctt cttagcgaat acttgcctct aggttttcta ccaacgcttt ttcgactttt
    28861 tccattgctt tattgatttc ttcatctgtc aatgttgctt ctgggttgac gaatgtcagg
    28921 ctatacgcca tggatttatg tcctttttca atgttttctc cttgatagac atcaaataat
    28981 tggatatctg ttaaaaatct tccggcagca gaacggataa cttctgttaa ttcctgattt
    29041 gtcacagttt cttttacaag caaagcgatg tcccggctga ctgctgggaa ttttgtcact
    29101 gcttcgaaaa cgatcccttt ttgagcagct tccactatag cagataaatc aaactcagca
    29161 acatatgttt ctggaatatc ataacttttt gtaatcgttg gatgcacttg accgacaaaa
    29221 ccaataaatt tttcgccaag gtaaatcgca gcagttcttc caggatgcat ttccttcatt
    29281 tctgtcactg tttgataatg gacatcttca gtgatgccta gttgttcaaa taagttttct
    29341 aaaagaccct tgatggtgaa aaagtctaca ttttgagctt tcgtctgcca atctttttct
    29401 tcccaaatac cagaaatagc gattgctgca tgttttactt cttgcggcag atgtgtacgt
    29461 ggatcatttt cttggaagaa cacccgacca atttcatata aagcaatctc tgtatttttt
    29521 cgggcagtgt tataactgat atcatctaat agtccgctga tcaaattcat tcgtaataca
    29581 gaacgttctt cgctcattgg ccagtccaaa cgtgtaatat tactttcatt tataacaaac
    29641 tgtcttgatt tttcttctgt tgtcaatgca taactgattg cttcgctcaa tccgcttcct
    29701 tcaaagattg tacgaatttt acgagtagtt tcttgttctt ttgacaagct tccagcaacc
    29761 gtttctccac ttggaagtgt agatggcaac tggtcgtatc cataaatacg agcaacttct
    29821 tcgataatat ctgcttcgat ttggatatcc cagcgtcttg gtggtactac tacggaataa
    29881 acatactcct tttcttcata gctaaatcca agtgcttcaa aaatctcatt tacttcttta
    29941 acgacaagag atgttcctaa atattgattg atgcgttcaa gtgaaatctt cactgatacg
    30001 tcttctgctt tgatctctga gcctttgacc gcgccttgta gaacttcccc acccgcaaga
    30061 gaaacgatca ttgctgcagc aacctcactg gcttctccaa cagtcgcatg gttgattcct
    30121 ttttcaaaac gtgcagagga ttcactacgc aaattaaatt gttttgctgt acgacgaatc
    30181 gaaattggat caaataaagc agattctagt gcaactgttg ttgtttcatt cgtgatctct
    30241 gagtctaacc ctcccatgac accagctaaa gcaactggtt ctttgccgtt agtaatgacg
    30301 agattgtcag ttgtcaattc ccgagattct ccatctagtg ttactaatgt ttcattttct
    30361 tttgcacggc ggacaaagat ctcttttgat ttcaatttgt cgtagtcaaa tgcatgtagt
    30421 ggttgtccga ataaaagcaa aatatagttc gtcacatcta ctacattgtt gattggacga
    30481 atgccttcgt tcatcaaaag attttgcagc cattgaggac ttggctggat tcttactcct
    30541 tcaatgattc tgatttgata agcaggaact gcttctttat cttcaacttc cacttgaatt
    30601 ttatcgctag caggacgtga ggattctacc agcttttcgt catcgaatgt aggagtctga
    30661 cgataaattg ccccaacttc ataagctacg ccacgcattg acaatgcatc cgcacgattc
    30721 ggtgtgatag ataattcaat aatcgaatca tccatatcca aataaggaaa aactggatcg
    30781 ccatttacag cttcttgtgg catataatag ataccttcag cgtattcttt cggtataaca
    30841 ctatctgaat agccaatttc ttgtaatgag caaatcatgc catttgatac ttggccacgc
    30901 atttttcctt ttttgatttt tacattacca gcgatacgtg agttaggaag tgcaacaatc
    30961 actttgattc caaccttcac attaggcgct ccacaaacga tttgagacag ttcgtcttcg
    31021 ccaatatcga cttgacaaat agaaaggtga tcagaatctg gatgtggtac gcattccttg
    31081 acttcaccga caacgatttt tttcaagcct tcttctggaa ccgttactcc ctctacttcg
    31141 attcctgtca cagacatctt atccgctaat tcttttggtg ttacattcga taaatctaca
    31201 taacgattga gccatttata tgaaactaac attttcttac tcccccttga actgacttaa
    31261 gaaacgtaag tcattttgat agaaattacg gatatcattc actccatacc gcaacatagc
    31321 tacgcggtct ggtccaaggc cgaaagcaaa gcctgaatat tcttctggat cgattcctga
    31381 catcttcaac acattcgggt gtaccatgcc agctcctaaa atttcgatcc atccagtgta
    31441 tttacatacg ttacaaccgg caccaccaca tttgaaacag ctgacatcta cttctactga
    31501 tggttccgta aatgggaaat aacttggacg caaacgaatt tcacgatctt ccccaaacat
    31561 ttttttcatt acgacttcta atgttccttt aaggtcgccc attgtgatgt ttttatcaac
    31621 aactagccct tcaatttgat ggaactgatg actgtgggtc gcatcatctg tatctctgcg
    31681 gaagactttc cctggcgaga tcattcgtag agcaccctta gagaaatcat gtttttccat
    31741 tgtccgtgct tggactggtg aagtatgtgt ccgaatcaat atctcgtctg aaatatagaa
    31801 agtatcttgc atgtcccttg ctggatgatc ttttggcaga ttcatccgtt cgaaattgta
    31861 atgatcagat tctacttcat atccttctac gatctgatag cccataccca caaaaatatc
    31921 ttcaatttct tccatgattt gtgtcacgat atgacgtgta ccatgtttta cttttctacc
    31981 aggtagtgtc acgtctattg tttcttttgc caaagctgca tttaacgctt cttcttctaa
    32041 tacttgtttt tttcgcacga tctcttctgt caaaagatct cggatctcat ttgcgaaact
    32101 tccgactttt ggtcgctctt ctggacttag atctttcatc ccacgtaata cttctgtgat
    32161 cggtcctttt tttcctaacg tttcaacacg tatttgattt aattcattta aatcttgtgc
    32221 cgctgtgatt ttgacgagcg ttgcttcttt caagctctct aattgtgctt gtagagacat
    32281 gtgcttgttc ctcctttaaa ttccaaatac aaaacaaaaa gacctcattc ctgttaagga
    32341 acgaggtctt cgcgttacca tcctaatttg cggataaatg acttacaccg cactcttact
    32401 tcgttaacga caattgccgg tctgatttta tcagacgaac tcggaaagtg aacttcattt
    32461 ttcaaacaca ggatctgctt tcagtctgcg acagatctcc ctgaactggt ttagccaaac
    32521 tactcttttt cgtcatcgtt ctttacttat tcgtatggac tatagtttac aagatttttt
    32581 ttacaaaatc aaccgcattc ttcctctgtg atccattttt cgctccaaac ttgtatttga
    32641 cgcatcgctt tttgcaagtc tgctcctttt tcagtcaaga gatattctga acggctcttt
    32701 tcgctacagg caaagtttcg agtgactatt ccttcttttt ctaattcctt caagcgttcg
    32761 actaatactc gatcactgac catcgggatt tttgccgcaa gctcaacaaa gcgttgcgga
    32821 ccgttttcga gtagtacatc gataatcaat ccgttccatt tttttcctaa aatagagaat
    32881 gttttttcaa atttagggca taaaacgaat tctgtctgtg ttagctccat catactcacc
    32941 tcttcccaga gtatagcata atccttacaa aacataagtc aagtttgctc aattataatt
    33001 atttgttaat gtttataatt tgtttcactc gaagcaaaat taggaaaaca ttttctactg
    33061 gcttattcaa agcttctgac attgcttgtt ggtaaagtct tagctgtcct tgatattttt
    33121 ggacgattgc atctatttct gctggatcat ccggatgtaa aataaaatct gttttaaaat
    33181 catagatatt gagatgatct ttttcttcta ggtatccatc gacgattccg tggattaata
    33241 gttcatcttc ttgatttgga tagtcttgaa atacttcgtc agcaggaagc agcatggaaa
    33301 aaggttgttc tcgttttacg ttttctttgt tggcaatcag ctgttgacca agttctgttt
    33361 gaaagaacca tataatagaa gaaacatcta cttttttcgc gactttttcg tctactaacc
    33421 gtttccttac caattcctgt aatttttgat ggatactctc tgtagttggc atctctaatg
    33481 gcagtaattg taacaaagca tgcgtagcag ttccaacagc agctgctgac acttgccgat
    33541 ctttttgcaa aaattttggt tctgctagtt gttcttgcgt ataacgaaat tgacgggatg
    33601 ctgctttgtt ctgactgctt tccagagtca aacgtgattc ttgtgtatca tctggatctt
    33661 cgaataatcg tttgatttca gatacagatt gataacttgt ggtttgtgaa gaagcttgat
    33721 aaggatattc atatgccaga cgtttttgta acgaatctgc taataaagtt tcatcttctt
    33781 tcatctgctg atctaagtta accagttctt tttcagcaag caactgtctt tgttcaatga
    33841 tcgattgctg attcatccat gaaatagaaa actgtgcatt tgaatgttga agaagagaag
    33901 tcgaaatttc ttcctctaag tatttctgca tttcaggatg acggatcaat ccgtaaccaa
    33961 tccaattcat caagctgctt ctgcccttca aacgtagcgc aggatcaaaa actaactcct
    34021 catgatcagc tgcttcagac cacgtttgga tcatttgctc tttattttta taggaaccga
    34081 caatgaaaag cttttgttct gcccgagtca atcctacata caatttacgc atttcttccg
    34141 acaacaattt gttttgcttt gccaatttga tggcttgaaa cggtaacgta tcatataaca
    34201 ctcgtgtttc aggatccata tagcgaattc ctgctccaag tttttcttca aaagcatacc
    34261 gattgcgcaa gtcttgtaag ttgaactcct ttgtcatatc taacaaaaat acaactggga
    34321 attccaagcc tttacttgca tggatcgtca taacgcgaac agcattatct tctattgaaa
    34381 tgactggttc tgctaagtct ttatcttttt cttgcatttt ttcaatgaaa cgcacgaact
    34441 ggtataatcc tctgaaagaa ctttgctcgt aagctttggc acgatcaacc aatgcataaa
    34501 gattggcttg tctctgtgcc cctgctggca gaccgaccac atactctaga tatcccgtct
    34561 cataatagat atcccataac aagtcagtga tcgaagaacg gcgagcaagt tctcgccaat
    34621 gctctagttg cttgccaaaa tgttccagct tagctgctaa ttcatcttcg tttgatgctt
    34681 gataagcaag cacagcatca tagtaagtat gtgcacggtt agctaatcga atgctggcta
    34741 attctggttc aatcagaccc acgatcggtg agcgcaatac agccgcaagc ggaatatctt
    34801 gatagggatt atcgattaat tgtagcagcg agatcatcgt gcgaatttct gtcgcttgaa
    34861 aatagttttg agcatcattc atctctaatg gaatatccaa tgttttgaaa atctctaaaa
    34921 tcgttaagtt gttcttcttt gtaggtgtca agagaacaat gtctttgtac tctatcggac
    34981 gacttttttt tgatttttta tcataaatca taaaggagga atcaattaat tgacgaattt
    35041 ttagtcctgt cataaaaagt tcgccttctg ttttgtcttc cagtatatca tccgtcggaa
    35101 tctcaatctc tgattcttcc tgttcttttt catagatcat gatttccggc tcaaactgtt
    35161 cattctctgg aaagtctgaa aatccttgga tcagtttggc tgcttcgtca taattgattt
    35221 gacccactgc tggatccatt agttgttcaa aaataagatt cgtaaaagaa aggacttctt
    35281 ttctagagcg gaagttctca gccaaaacga ttcgacgtcc gccttctttc tttgagaaat
    35341 tctcgtattt cccgataaat aaactaggat ctgctaaacg gaaagaatag atggattgtt
    35401 tgacatcccc aaccatgaac atatttcctt ttgtatcatc gggttctctt agccagtata
    35461 aaatggcttc ttgtaattgg ttcacgtctt gatattcatc taccattact tctttgaatt
    35521 tttttcgata atgttttgaa gcttcacttg gcagccatac atctttcgtt ttttctgtga
    35581 gaatctgcaa cgctaaatgt tccagatcat tgaaatccag cacacctttt tcccgtttac
    35641 gcatactgaa accgtccatg aagctttgcg tgacttttgt catttcttcg actaacggta
    35701 gtgctttgtc cgttaattct tccatttggc ttggggaaac aggaaaataa cttttactta
    35761 tctgctgaat cagttttttt gctttatccc tcatgccctt gacttcattg gaaatttctt
    35821 tcaattctgc tttccgactg ctcttaaaag ttgaaaaagt tagttgttcc aaaccagcat
    35881 aagcttcttc taaccgatct tgcgagaacg cttcatagat attcttgatc tgctgttgtt
    35941 ctccagctac ttgctcatgc attttttcta atccttcacc ttgtgctaac tgagtcatct
    36001 gttcatataa ctgtacacat tggtatatat ctgccaaaac taaaggacgg atttgttttt
    36061 gatataatcg agatttcgct agcccctcag gcaaacgata attatcgctt aactgttcta
    36121 gccatttttg ggggtcaggg ttggcacggg caaactcata aagagaaaac accagattcg
    36181 tcaagccgtc atctgaacga tcattagaaa aattcattgt taattggaaa aatcgttcat
    36241 cattttctgc ataaagtgct tctctcagct cgtcccacac atcctccttc atcaagatcg
    36301 tttctgtttc atctgttaac atgcgaaaga ctggatcaat atcaattaag taataataac
    36361 gtcgaataac tgtcaggcaa aaagcatgta aggttgagat attcgctgtg ggcaataaaa
    36421 ctaactgctt agtaaaatgc tgtctacgta cagggtcact ttcgctattg acggattctt
    36481 gtaatgcctc ttgaattctt tctttcattt ctcttgcggc agcttcagta aatgtcacaa
    36541 tcaataattc atcgatatca aaacccattt ttagtttttc aatcactctt cgaacaagca
    36601 ctgtagtttt acctgatccg gcagacgcag agactaatag attatctcct tgatcaaaaa
    36661 tcgcttgcca ctgatcatct gtgaatcgtt cattttgcgg ttttaaagga atttgattca
    36721 ctcttcttca ctccttttca atattctacg cagtgcttct tccttatcta ttttctccag
    36781 acggtggtaa ttattttctt tcagcatcac atcaaacgta cagatgcttc tgaatggaca
    36841 aaattcgcag gcaatccgct gtttatcttt ataagctgga tttagtttga tttctccgga
    36901 agtaatcgca tctccagcta accgcatatt ttcccgatta tgcgccatga atagttgcag
    36961 ttcttcttcc gtgaaaaatt tgtctttact gaaactacct ttttggtatt catctttcgc
    37021 attttttttc actggataga tcagtgattg ttcttttgct tccagtgaat gatctagtac
    37081 ttctaaaaga gcgggatcat cgacaaaaac gccatcataa ctaaattttt tcaatgtatt
    37141 cttctctaca tcatctgtct ccgtcaatac aggattatga acatgtaaat aatatgatcc
    37201 tgctggttta acagccgcct ttccagtcaa acgtaccgca tccatcaatg ctacgtccag
    37261 atacgtcaat aattgcatag ccatgccata ataagcttct gtgacatcga atgaacgtcc
    37321 gctagatttg taatcaacga cgcttagcca agtatcttct tgttcagaag ctacatcgat
    37381 tcgatcgatt ttccctcgaa catggagttt tccaccatta ctcaacggta attccagacc
    37441 tgtgatacct tttgctccag caatctgacc aaatagaatc tctgtctgaa gaggatgcat
    37501 gcctgtattt ttgctctgtt tttgcaaagc ccatgtcact ttttgaatcg tctgtcctaa
    37561 ctggtagcgg atatagttca ttcttgcaga actatccaat atctcaaacc gaagttctcc
    37621 gaatacttcc tgcagcactt tttcagtaaa gtctctacgc tgcgcatcgg acatattcac
    37681 taaggataat tggtttgaaa acagtagctt gaagaaacga tccaatgatt catgataaaa
    37741 atcaccagtt gctgctggag ttagaccata aacagttcgt tctttcaatt tcaaaccaaa
    37801 ttgcatgaag tatttgtatt cacaattata aaaattctcc atacgtgaaa ttgaagtata
    37861 aatctctttt ccataaagct cttccgccat tttttctggc atcgaaacag gaacatttaa
    37921 atgttcctga ctttcaaaaa tctgtaaagc aaaatttcgc catgacgaat tcagtactaa
    37981 ttctttgagc ttttgccagt gctttggtac aggttttttt tcatctaaag ctaatcgata
    38041 aatcctattg actgtattga ttccgcttct gtaagtccct acataacaat tagggtcgct
    38101 ttcgatcgtc aaatgctctt ttctctcgac gggaacagat aaatattctg tgattctacg
    38161 caaataagga gacatcttca gcgaaccatc tgtgtcatag ctttgtgcat aactaagata
    38221 taatcgatct gttccggaaa gaaaaacaag ataggcttgg aaaggctcaa aagagatact
    38281 ttcctttgat gggtcgaata aaaactgtcc atctggcaag gcctgattta atacttctcg
    38341 ttcttcactg gacaacaatc cttttgtctc ttttcggtct ggaaacactt gatcattcaa
    38401 ccctaatgca aaagtgacgg cagcttgatt tggtcgaact aattccaaac gattgattcg
    38461 cacttggtcg attgcggtag ggattttacc ataacttaga ttttctagac cacttgtaat
    38521 gatttcttgg aacagtgacc agtcaaaagc cgattcacca taaattacta catattcatc
    38581 caacagatca atcagcgctc cccatgtctg ctcatggttt cttgccgctt ctaagtttcc
    38641 tctgtctact tcctgattac gccaaaacaa taattgttgc tgtacgccac tttggatcaa
    38701 aaattgataa aaaacatttg cggcctcttg tcctgttttg cttttcttta tttttttcaa
    38761 aaatactggt acagctttct gaaataatcg tctaatcgta tttgactgtt cttccaaata
    38821 ctgtacgtct tcaatctgtt ctgcttcaaa atcatacgcc acaaactgcc agtctttttc
    38881 acgagtccaa taagttcctt gataattgta agcaagtgct acattttcag tgatgtccag
    38941 ttttttcctg aattcatctc gctcttgttt ccattcatca agttcggttt ctgcttccgt
    39001 cgacagaaat aattcagttt tgaaaaaacg cataatatca cttagccgat aagaatagcg
    39061 gtcaagagcg aacagactgt tgatccattc gatcagcgga tgattttcca tcatttgttc
    39121 ttcatcgata taaaaaggaa ttgccatttc ttgaaagatc ggccgaagga tatttccata
    39181 taaagacata ttttttgtca gcacttggat atcacgataa cggtagtttt cttcactgac
    39241 tagtcgtctg atttcaacag ctaattgtcg gacttcttca gttggatttt cagcttccca
    39301 caaatggaga tgctcttgtc cccctatttt gtgtactgcc gctttctttt ggctagatgt
    39361 ctcttcccac attgtttgca gcagttgaag ctctgtcgga cgagtctgct ctggtgcaaa
    39421 tcggtccact aatataggga ctttccgttc tcttgcactt tgaaaaagac ggaaataaag
    39481 ctttccagtc tcatgaaaga gcgtaagtac ttctggaaga tccgctgtat aaggacgatc
    39541 cagcaataaa tcgacaacca attgcccctt ttccatcatt gcctgcagta attgatactc
    39601 ttgaccattg attcgagaaa aaccactgac aataaatttg acgttgttga aatcttgtgt
    39661 ctgcatataa tttgctagta tagatagtgc atcttcattt gttagtgcac gttgagtcaa
    39721 ttcgctgtca taagctgaaa aaatcagctt caagtctttc attttaagtt gctggtcttc
    39781 tgcattaggt gaatcaccaa ataaaagcat caggtcttct ggttgaatat ttcctgtacg
    39841 caattcactg tagagttctt gtaattgacg aataaaccct cttttattga tttctccgcg
    39901 aaagattgtt aattcttcct catttttttc taaaatctgt cgcaagatca ttgcagatcc
    39961 tgattctgat aattcatttc ctgtaagaag agttgtctgt tgcagcaaat accaagccag
    40021 acgataaaaa ctaaatactt gaacgtttgt tgtgctgaaa gttttttttc cgcttcttct
    40081 tcgcatctcc gctaaaattt cttgttcctg ttcgaatttg ctgtagttgg gaacaaggaa
    40141 aaatacttgg ttacttgcat cctggtcgag ccaagcggat gcttcgtcga tcaagacttg
    40201 acggtgatct tttttcccgt ttccaagaat aaattgtaaa gacatgcttt tcctccttca
    40261 acacatgata cggtgctcct ttattatatc aaaaaatact gaacatttgt tctatattct
    40321 tcaaattata tccttttaag gttctgatat ttctactttt ttaagtatac
//
LOCUS       DACDIE010000020        39891 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.020, whole genome shotgun sequence.
ACCESSION   DACDIE010000020 DACDIE010000000
VERSION     DACDIE010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39891
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.020"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(16..708)
                     /locus_tag="IV250_001147"
     CDS             complement(16..708)
                     /locus_tag="IV250_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP8768446.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            703..969
                     /locus_tag="IV250_001148"
     CDS             703..969
                     /locus_tag="IV250_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8768447.1"
                     /translation="MHRTFGSRGCPTLHLADRYAERKTPTLCFAFSARTRQAGIPLED
                     IKDFLGHKDVSTTQVYAHISPEVKKRSMNQLENYIEEQIKKHSN"
     gene            complement(1145..1714)
                     /locus_tag="IV250_001149"
     CDS             complement(1145..1714)
                     /locus_tag="IV250_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP8768448.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1788..2111)
                     /locus_tag="IV250_001150"
     CDS             complement(1788..2111)
                     /locus_tag="IV250_001150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8768449.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVITDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2265..3077)
                     /locus_tag="IV250_001151"
     CDS             complement(2265..3077)
                     /locus_tag="IV250_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8768450.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3431..4279
                     /locus_tag="IV250_001152"
     CDS             3431..4279
                     /locus_tag="IV250_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAP8768451.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPRPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4424..5365
                     /locus_tag="IV250_001153"
     CDS             4424..5365
                     /locus_tag="IV250_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HAP8768452.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLKKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKAFFTHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5355..7526
                     /locus_tag="IV250_001154"
                     /pseudo
     CDS             5355..7526
                     /locus_tag="IV250_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(7638..9809)
                     /gene="copB"
                     /locus_tag="IV250_001155"
     CDS             complement(7638..9809)
                     /gene="copB"
                     /locus_tag="IV250_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HAP8768453.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(9829..12015)
                     /locus_tag="IV250_001156"
     CDS             complement(9829..12015)
                     /locus_tag="IV250_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAP8768454.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(12015..12224)
                     /gene="copZ"
                     /locus_tag="IV250_001157"
     CDS             complement(12015..12224)
                     /gene="copZ"
                     /locus_tag="IV250_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HAP8768455.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(12237..12677)
                     /locus_tag="IV250_001158"
     CDS             complement(12237..12677)
                     /locus_tag="IV250_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAP8768456.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12751..13290)
                     /locus_tag="IV250_001159"
     CDS             complement(12751..13290)
                     /locus_tag="IV250_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8768457.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
     gene            complement(13905..15371)
                     /locus_tag="IV250_001160"
     CDS             complement(13905..15371)
                     /locus_tag="IV250_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8768458.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            complement(15682..17763)
                     /gene="cadA"
                     /locus_tag="IV250_001161"
     CDS             complement(15682..17763)
                     /gene="cadA"
                     /locus_tag="IV250_001161"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP8768459.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
     gene            complement(17905..18084)
                     /locus_tag="IV250_001162"
                     /pseudo
     CDS             complement(17905..18084)
                     /locus_tag="IV250_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308695.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
     gene            complement(18288..18647)
                     /locus_tag="IV250_001163"
     CDS             complement(18288..18647)
                     /locus_tag="IV250_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAP8768460.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(18677..19018)
                     /locus_tag="IV250_001164"
     CDS             complement(18677..19018)
                     /locus_tag="IV250_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8768461.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(19015..19689)
                     /locus_tag="IV250_001165"
     CDS             complement(19015..19689)
                     /locus_tag="IV250_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP8768462.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKSLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(20741..22039)
                     /gene="asnS"
                     /locus_tag="IV250_001166"
     CDS             complement(20741..22039)
                     /gene="asnS"
                     /locus_tag="IV250_001166"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAP8768463.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(22079..23269)
                     /locus_tag="IV250_001167"
     CDS             complement(22079..23269)
                     /locus_tag="IV250_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP8768464.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(23290..23817)
                     /locus_tag="IV250_001168"
     CDS             complement(23290..23817)
                     /locus_tag="IV250_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP8768465.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGYESGDEVKTIKGI"
     gene            complement(23864..26653)
                     /locus_tag="IV250_001169"
     CDS             complement(23864..26653)
                     /locus_tag="IV250_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP8768466.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKATLKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(26800..27000)
                     /locus_tag="IV250_001170"
     CDS             complement(26800..27000)
                     /locus_tag="IV250_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP8768467.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(27452..27772)
                     /locus_tag="IV250_001171"
     CDS             complement(27452..27772)
                     /locus_tag="IV250_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8768468.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHILHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(28275..29393)
                     /locus_tag="IV250_001172"
     CDS             complement(28275..29393)
                     /locus_tag="IV250_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8768469.1"
                     /translation="MAKKDKELLSQLISEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FSKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            29866..31173
                     /locus_tag="IV250_001173"
     CDS             29866..31173
                     /locus_tag="IV250_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAP8768470.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKVSGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANMFVIGWRRVAEFFGNLF"
     gene            31291..32490
                     /locus_tag="IV250_001174"
     CDS             31291..32490
                     /locus_tag="IV250_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8768471.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRVIFSSNNYHIF
                     RAGLYACQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(32517..33785)
                     /locus_tag="IV250_001175"
     CDS             complement(32517..33785)
                     /locus_tag="IV250_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HAP8768472.1"
                     /translation="MEKQLPSPMRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DTRLSHPWLLYLLPVSGALFAYLYVYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(33948..34595)
                     /locus_tag="IV250_001176"
     CDS             complement(33948..34595)
                     /locus_tag="IV250_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HAP8768473.1"
                     /translation="MEQTIKPYQYFFIKREVEQLLNAYSSVNDPKTVQTVQALAAEKI
                     RDILDHELLEIDTFLTTVLDVKLTKAQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQEHTYIGWNDPGTQKKFMVYYEDEKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKKER"
     gene            complement(35003..35506)
                     /locus_tag="IV250_001177"
     CDS             complement(35003..35506)
                     /locus_tag="IV250_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8768474.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(35629..36393)
                     /locus_tag="IV250_001178"
     CDS             complement(35629..36393)
                     /locus_tag="IV250_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAP8768475.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLADVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            36500..36775
                     /locus_tag="IV250_001179"
     CDS             36500..36775
                     /locus_tag="IV250_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAP8768476.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            37371..38321
                     /gene="yidC"
                     /locus_tag="IV250_001180"
     CDS             37371..38321
                     /gene="yidC"
                     /locus_tag="IV250_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP8768477.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPADNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(38500..39474)
                     /gene="lacD"
                     /locus_tag="IV250_001181"
     CDS             complement(38500..39474)
                     /gene="lacD"
                     /locus_tag="IV250_001181"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP8768478.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDTKMPGR
                     LPDLLNIWSVKRLKEAGADACKFLLYYDVDEPAEINVQKQIVIERIGSECEAEGIPFF
                     LEILSYDTTIGDPSSLAYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QETCYSQAEASAYFYEQSKVTEQPFIFLSAGVNATLFQDTLRFAKQSGSTFNGILCGR
                     ATWAKGVAPFVTEGEKKASSWMLEEGKQNIKELSTLLKETATPVYVDR"
     gene            complement(39471..39644)
                     /locus_tag="IV250_001182"
                     /pseudo
     CDS             complement(39471..39644)
                     /locus_tag="IV250_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
BASE COUNT        11417 a         8385 c         7059 g        13030 t
ORIGIN      
        1 agaggggggg attttttatc gttgtggaat atttaaatac caacgcttgt catgaaaatc
       61 ttgtgctcca cccttagtat ttccttctgg atcgtttgtc gcacgcatca tgacgtatac
      121 tttcttatta gggaaatcac gcatattgaa agatacatga tagccaacat ttcctaaagt
      181 attataagct tgatttacgt ctggtctata aatcccatca gctcttactc tagctagttc
      241 ttttccagta ttgtagtcca taatgaaaat atactcgtat ttataattag caatgtgcca
      301 tccagctaca tgcaagtttg cgttttcgat ttctccgaac tgatcaatgt gggcgtaatt
      361 tgttccatct gtcagcgtag aatttgcagc acctgctcga gttggatcaa ttactggttt
      421 atcctccgaa gtagttggat tatcatccgt aaaaccatga gccaaatcat aggctagttt
      481 ttctttactt acacccattt cagaaagata accgtaagga tctgtatgat cgccccaaat
      541 attttgtgtt acccataaat gcgatttgat tcctggttgg ttataaggag tgtctaatgt
      601 taatggaata ccatatttca ttgctgaatc tcttgccaat tcaacgtata ctttatagtt
      661 cttttcaaaa gtttctttat catgtgtgtg ttgtaactcg atttgcacag gacttttggc
      721 tcacgaggtt gtcctactct acatcttgct gatagatatg ctgaaaggaa aacaccaaca
      781 ttatgttttg ctttttcagc tagaacgaga caagcaggaa tacctttaga agatatcaaa
      841 gattttttag gtcataaaga tgtttccact acacaagtct atgcccatat ttctcccgaa
      901 gtgaaaaaaa gatcaatgaa tcaactagaa aactatatag aagagcaaat aaaaaagcac
      961 tcaaattgag tgctttttta agctcactat cactaaaact atcactaatt ttatttataa
     1021 gcgaacatgt ttctatcact gattttttaa atctagcgaa aaacccttat cttaaaggcg
     1081 ttcgttcaat tctttggcaa ggtcttcaaa gcctggcttg cctagaagtg cgaacatggt
     1141 tttcttatag ctcatgtaga aaaattttct tgatgatgat tttatccatc aattgagatt
     1201 gactgttttg ctctcctatg atttcagcta tgattgtccg attttgcggt tttcttctta
     1261 actggtagtt gattttccca gtttccatca actcgctaag catatccacg actccgccaa
     1321 atccggtctt tttcgtaata aaacctagtg ctaccattcg ttcatctaga ttttttgctg
     1381 taatcgtcac accagtaatg agcgagtgct gtaatgtttg ggccagctct tccatccctt
     1441 cagcattgga tacgacggaa gctgttttgt taataggttc ttcgtttatt tcttttggtt
     1501 ctttcctctc cactttcact gtttgacgaa ggacatctag ttcttgcaag agcgtttcgt
     1561 attgttcttg ggtcagcatc acgccagcta ctttttcacg attaaatata tatacgccag
     1621 ttcccgcgtc tctcgcttga gcaaatacat ccatcggcga tcgtttcact tctgtgatcg
     1681 aagatgtggg aacttctaat ttctttaatt ccaatggatt tcctccttta ctaatcgctt
     1741 tttattatag cacagactga cttgttttga tcttttttcc aaaggtttta tcggtttttg
     1801 cctaaccagt aattgatatc ggtaagaagt ctttcgatac caaatgaagg atggtacaga
     1861 tacgtttttt gatcttcgtt atggtctagt ggaaaaaaat gatcggtgat cactagatct
     1921 gcataccgag gcggcttatt tcgagtacat acatgtatag agatccgttt tcgatgtctt
     1981 agcgttttgt tcagtacccc agcaagtctt cttgcttctt ggtgttcata gcgaaaaaaa
     2041 aagacgattt tcacttcagg agggtggaca tctaaatacc ctcgttcctg gagtgttttc
     2101 agtaaaagca tcccccactc tactgcgggg tgtgcaagtg cttgttggat cgctagccga
     2161 aattgatata gtttatcttt ggaattggga aatttttcta atgtttcctg ataataacga
     2221 acttcttcta tcagccggtc atggatacca attacgtaac gaagtcattc ttggtaaagt
     2281 acttttgtca gctttgattt gctttcgctc tcaaattcgc tattcagcca aaacagcgaa
     2341 tcaagctttt cagtcacaat cgtcaatttc cgatagtctt gtgtctcttc tatccgtttc
     2401 caccgctttt cagtaatctg gttacagctc aagcgcaaaa ttgctagata atttttttct
     2461 tttattggca aaatctgctc ctctctaaat agcaattcag gaaacaattc aaagaatagt
     2521 gttcgactgt ctcctaaaat ctcgatttct gccggatcct ttctttgctt caataattgg
     2581 atgaacatct cagaaaagga ctgctctcca tcatctgcaa aaaaatgcgg aaaatctttc
     2641 aaaaagcaaa acagcagata tcttactttt ctttctgaac caaagatctg tgctttttta
     2701 taagaaaaag aaaggtggta ctcgccaaac cactttctca atcgctcttg cgtagaacga
     2761 tcgtacttct gctcgtaagg ttcattcaat agcagatttt tcagtaaaca aaacgggggt
     2821 gattggatca gaaaaacaaa caagagataa ggttcgctat atccttgatc ataaactaac
     2881 tgaacggttt tcttatgaag aatcagctga atgggacagt cgctttccgc caaacgtttc
     2941 agatgccgat ccactgtttt aacatcgatt ttcagttctt cagctaaatc agcttttcgc
     3001 cagatcaaat ctttcattag attgattagt gaaaataaat tctgtgcatt ttttgataat
     3061 agtttttgcc atctcatcga tcatttctca cctgatttct aaacatattg tttgatcaaa
     3121 atgggttcac tcctaaaaag ataaaaaact ccctttctat ttatttaaat acgcaaatct
     3181 tcctcattat tttttatttt actcaattta atcgtattct catttcaaaa cgaatgtact
     3241 ttaaaaaaac ataataagat cactttgctt gtaggatgta ttcccttcca ttacaatgaa
     3301 ataaatagtg attttatgag aaggtgatgt gaaaatggaa gaaaaaatag aagaatccaa
     3361 acaaaaaatc gatcagttga tataagaatt agaaaaagcg gtcatcggca aacgttcaac
     3421 tatccaattg atgatcgtgg ccctattagc aggtggtcat gtcttattcg aagatattcc
     3481 cggtgtaggt aaaacgttac tggtcaaagc actagcaaaa gctgtacagg gaaccttcag
     3541 cagagtccaa tgtactcctg atctgctgcc tagtgatatt cttggttttt ccgtatacaa
     3601 ttcacaatca aaagagtttg aatttcgtcc gagaccgatc tttaccacga tcttgttggc
     3661 agatgagatc aatcgtacga ctcctcgtac tcagtcagca cttttagaag caatggccga
     3721 gaatcatgca acgatcgaca acaatacata ccctctagac aatcattttt ttgtcttagc
     3781 tacgcaaaat ccaatcgagt acgaaggaac atatcctttg ccggaagcac agctcgatcg
     3841 ttttttattc cgactacaaa tcggttatcc ttcttttgat gacgaacttt tgctactttt
     3901 ggataaattc gaaaaagtgt tggataactt aaatgaagta ttgaacagtt atgaaataga
     3961 agaaataaaa acaaatgtgg cgaatgtctt tgtcagtccc gaagtggcat cttatgcctt
     4021 gcagttagtg tcagcaacaa gaagtcatgc tgccatccag cttggcatca gtccacgagg
     4081 aagcctttcc tttattcagg cagcaaaagc ttatgcttta atccatggga gaaattacgt
     4141 cacgcccaaa gatttgcagg atttagttcc ttacgtgttt tcccatcgcc tgatttttta
     4201 cgatcgttca ttgaaggaag acgaaaagaa acaaatcatt catactcttg ttgcacaaat
     4261 tcctattcct gtgaggtaga ttgaggtaga caatggaaaa aatcaaagat tatttcatta
     4321 attttgtgtt atttctggta tatttcatca ttgtgctttt tgctcttgta ttcaacaatt
     4381 ctttaggctg gttcttgttg gcctttttta ctctttatgt atcatggagc ttgatttcat
     4441 taagcagctt tctaaagaaa ctttcggtta cttgtacatt gcagccactc gtttatcgca
     4501 aaaaaagtat gcaggttatt ttttccatag caaaaaagag aactttttct ttacttctcc
     4561 ctcgtttaca aatcggcttc ccagcaatct ttgcagaaaa aaacaagaga ttttgattct
     4621 tacaaataaa ccgcaagaag tccaattgct ttggcagcca aaagaaagag gatgccatga
     4681 aacattaact gtcattttta cagcctatga tagtctagga cttttcagaa aaagtgcaaa
     4741 aagagagatc tcttttcctg tgactgttct gcctgctttt tgtaagattc aagcagaaaa
     4801 attacagaga gttctggaaa aaaaacagca gctgaacgcg taccaaagag gtcttgattt
     4861 tcgggaacat cgcgcttatc gtccaggtga ttctttcaac cgaatcgatt ggaaattgtc
     4921 tgctcatact caagagtggt tataccgtga atacgattat caagaagaag aatgttctcc
     4981 tcttttttgc ttttggggtt cgcccagtcc aaaatttgaa cctctgctag acttgtattt
     5041 ttctttatgg catttgaaaa aagaaaagca gccggaactt ctgatattag gcaaccgcgc
     5101 ctttcatggc aaagaccccg gtcataccta ttttgcttct ttggaagctg gcgatgagaa
     5161 agcttttttc acacacttga aaaaatatgc aaaaaaacaa atcgtcctct ttgttcctga
     5221 gaattctcct gaagtctcag aagctattga aacattgtca aaagatcgaa cggtttatct
     5281 tgtttatttc gtagaaaaac aactcatggt ccaagaaaaa gacaagatat gttcattgcc
     5341 aggaggtgaa tggattgacg attaaacggg aaaaatggct gcgtggcgtt tactcctttg
     5401 tgatactaag tgtaggtgct ccgatatttg tagtagctaa tcaattgcaa aacagctacc
     5461 ttcttatcct aactattggt gtcctatgcc ttattgtctg ctgtattcct aaaaaatggg
     5521 tacgtttgct gctttctttg ccagtaatga taggttgttt gtttttatat tttccttcga
     5581 agacattttc catattatgg atcttacagt ttttttcgtc agttacagag gaattcttcc
     5641 atctatccca aggtaaactt tattatttgc cagaaaagac agcttttttt ctcataatgt
     5701 tgctgatcta tttatttatt acattgaccg tggattacga ctattggtac gcaccctttc
     5761 tttctttgat ggcttacttc atgatgctta ccgtttttcg gaaaaaagat ttactgtacg
     5821 aaatgattgc ggtagtaact gttttgttcg tgtatctggc agcaaggcaa tttttctcca
     5881 tccggttgtt gtcgggaaac actcgattcc agtcattgac tacagttctg cttatttgtt
     5941 tcttaacttt tagcgctatc ttgcctttat atcttccgaa agtacaccaa tctctcgagg
     6001 aaacgagtga accgatccga gaatatctta atgatgaagg gctttatgac acactccatg
     6061 agtatcaatt aaccggaagt gcaagaacag gcttcagtga gaatgatgaa cagcttggtg
     6121 gacctttata tgataatggc gaagttgttt ttacagcgaa ccaagaagga aatggctatt
     6181 ggaagatcga aaataaaaat cattacaccg gttctggatg gatagaagat caggacgcac
     6241 ccatccaacc aatccaagaa ttccctgttg acatcacgag ctaccaaacc gttatagaag
     6301 acaaagaaaa cattacattg accctaaaaa aacaagtttt cttaccttat ccttatggaa
     6361 atactacctt gactgatttt tcattagcta gtggacaaac gctttactat ttgcctgaaa
     6421 gccagcgatt cattcctgtc ccgaatgaat cgacacgttc tgcaacttta caaacagaaa
     6481 ggttagtttt tacgagagaa gaattaaaaa tcagtcagtt aaaagctgat gatttagaaa
     6541 atcagcagac aaatcttcaa ttgccaaaaa gtttgcctaa acgtattgag gacttggcgg
     6601 tcgaattgac caaaaatgaa gcaactttaa ttcgtaaagt cgaagctgtc taacagtatt
     6661 taaaaacaag cggaaatttc cgctattcca aaacggatac cgggcatact ccaaaagaac
     6721 acgattatgt ggattatttc ctctttgatt caaaagtcgg ctattgtgac aacttttcaa
     6781 cttctatggt cgtccttctt cgaacacttg gtatcccagc tcgctggaca aaaggtttta
     6841 caaatgggct cagaacaggc acagataatc aagggaatga ggaatacagc attctcaata
     6901 gtcatgctca ttcttggcca gaggtatact ttcctggatt tggctggctt ccatttgaac
     6961 caaccccttc atttgccaat ccaatgggtt cacaaccagt tgctgaaaca accgaaaccg
     7021 tccagcctgc tatcgaacaa ccaaatgaaa gcgaaaatat ggcagaaact atttccagtg
     7081 agagatctga ggtcattcca tcagaagaaa caacactcac accagcttat aaaatcgtct
     7141 tattcagcct tggagtgtgt ttatccgtct tgttgttctt tctattgtta ttcagaaaat
     7201 ctctgcgctg gacaagttat cttatctatc ttcatttttt tgcttctact aatacgattg
     7261 catatcaaaa acttcttaac cctttggaaa cattggtacc gcgcaaaccc gcagaatctt
     7321 tgcaggaata tagcctgcga atagaacaag aattcaagct agatctgcgt ttcattcgat
     7381 taactactca atatgaagca gaaatatatg gacgacaagc agatccggta aaattcgata
     7441 aaaaaaccta tttagaaata acgcatataa taagaaaaat ggtatacaaa aaaattctga
     7501 caaaagggag ccgcttcaaa aaataagata aaatttctgg agaatctact tctggaatgc
     7561 cgagaaccag ctgattgagt atcgattact cgaaaaaaag gttgtgacac gttttttgtc
     7621 acaacctttt ttcattatta tctgattttc aatgtcaaag catttagagc tacaactacc
     7681 gtacttaacg acatcaagac agcaccaact gctggactta atatgatacc aattggcgca
     7741 agaattccgg ctgctagcgg aattgcaaga atattgtaac ctgcccccca ccagaggttc
     7801 tggatcattt tctttcgcgt ttgcttggct agatcaagaa aatgcaaaat atcttttgga
     7861 tcgctgtttg tcaatacgac atccgcagaa tcgattgcga tatctgtccc tgcaccgatt
     7921 gccatcccga ttgaagcacg ggctaaactt ggcgcatcat tgatcccatc acccaccatg
     7981 attacttttt tcccttggtc aaggtatttc tggacaacag cttctttatc atctggtaat
     8041 aaaccgccat aatattcttc gatccctaaa taatctgcta ctgcttgggc agcgttttta
     8101 ttgtcaccag taagcatgac aggagtgatt tttctttctt tcaatgttcg gataaattct
     8161 tttgcttctg tttttactac gtcacctaaa gcaacgattg cagctagatg attttctaaa
     8221 atcaggaagc tgatggtatt tccttgttct tggtaaggtt tcaataatgc ttgttctacg
     8281 tttaatccga gacgctcaac ttctttttca ttaactattt tcacttgttg ttttttgact
     8341 gttgcctcta gcccgactcc tgaaagattt tttaggtttt cagcttgata aggcttgatt
     8401 tcatgttctt tcaaataatt cataatgcca acagccaatg gatgattggc attcgcttcc
     8461 aaggctccta gatattgaag gatttcattt ttattgaacg catcatcaag tacttcgaca
     8521 cccgttacag taaattttcc ttctgttagt gttcctgttt tgtccagcat gatcacatct
     8581 agatcattgg cttgctccag tgcattgcgg tttttcaaaa gtaatccgtt tttagctgct
     8641 attgatgtgg aacgagcaac aactaatggg atagctaaac ctagagcatg aggacaggcg
     8701 atgataaaga ctgttaccat acgttctaat gcattaggaa gatctgtcaa gaacaaccag
     8761 gcgatgaatg ccaggatacc cacaattaat gcgacataaa acagccattt tgccactttg
     8821 tcagataatg actctagctt ggatttttct ccttgtgctt ttcgcaccat ttccataacc
     8881 ttcgctagat aaccattttc accagttcca gttacttcga tttcgatcgt tccatcccca
     8941 ttgatagaac cgccaatcac tttgtcattg gcttgttttt tgattcctct tgattcgcca
     9001 gtcacagctg attcgtcaac aattgtttca cctttcaaga ttacaccatc tgttggcatt
     9061 ttatcaccag accggacgat caaacgatca ccttcgttta cttctttcaa tgaaactgtc
     9121 tcttcttttc catctttcgt caagcgtttc actgattctg gcagtaattc tgctagtttt
     9181 tgtaaagcat tgctggcatt cgacacagca ttcatttcaa tccagtgacc tagtagcata
     9241 ataacgatca aagtagccaa ttcccagaag aaatccatga cgtgtgtatg tggattgagc
     9301 agatttgcga taaacgaata gacactataa atatatgcaa ctgtaatccc catcgcgatc
     9361 agtgtcatca tagcaggact tttttgtttc aattccatct tagctccact taaaaatggc
     9421 tgaccgccat agataaatag tatagttgct aagatcaata cgagccaatc agaaccagga
     9481 aacgttactt gaaatggaaa ttccattccc atcattggtg aaaaaagaat gattgggata
     9541 gctaggataa gcgatagcca aaatttttgt ttgaaattcc ccatgtgcat ggaatgatcc
     9601 atgccgccgt gttcgtgatc catgtgcatc gaatggtcca tttgtttgct gtttgctata
     9661 tctccatggt tatgtcccat gtgcatggaa tgatccatct gactatggtc atgtttcatc
     9721 tccatctgtt tttcatgaga ttctgtactt tctttgttta ctacttcttt tttatgctca
     9781 tgttttttct ccatatcgtt cccattcatt tttactccat cccttctctt actttttcat
     9841 gtgtcgattt aagctcaggg agttcaacaa tacactgacg gaactaaacg ccatcgctcc
     9901 tcctgcaatg attggattca aaaagcctag tgcggcaaaa ggaataccaa tcgtattata
     9961 gacaaaagcc cagaaaagat tttgtttgat ttttctcaat gtcaacctag acaactcgat
    10021 tgtttgtgca atggatgcca aactgctatt catcagcgtc acatcggctg tttccatcgc
    10081 aatgtccgtt ccacttccca ttgccatacc gacatcagct aatgctaagg ccggtgcatc
    10141 attgattcca tcgcctgcca taccgacttt tttgccatct ttctgaagtt tttctacgta
    10201 ggctgcttta tcctctggaa gtacttcagc aaagatatgc tttggatcaa taccaacttg
    10261 tttaccaatc gtttcggctg ccagtttgtt gtctcctgtc agcataaaaa catctaatcc
    10321 tttattctgg agctgcttga ttgcttgttt gacttctagt ttgatttgat cagccacggc
    10381 aatcaacccg atcacttgct tttcatcagc taaaaacatg actgtttttc cctgctgttc
    10441 taattctaac gcgtgttctt ggtattcatc aaatgatagg ttcaattcaa ttaatcgttt
    10501 acgtgtaccc gcaaaatagc gtactccgtt aatcgtaccg ctgattccag cacctgggtg
    10561 ggctgtaaaa tcaatcatat cgtatgttgc tgcttcttgc agttttccgt attccacgat
    10621 tgctttacct agtggatgtt cagaagcttg ttctaaagta taaaaaatag ataggacttc
    10681 tttagaaccg actagatctg taactttggg ctttccttct gtaatcgttc cagttttatc
    10741 caaaacaatg ctgtctaagt gagcagctgc ttctaatgct tctccgcctt taattaaaat
    10801 tccatttcgt gcaccaacac ccgttcctac cataatagct gttggagtcg ctaaacctaa
    10861 tgcacatgga cacgcaatga ctaatacaga gacactatgg atcaatgcga gttgccaatc
    10921 accagtgatc aaacctgtag ctatgagcgt gataaacgcg atgactaata cgatcggaac
    10981 aaaaatccct gagatccgat ctgcgatttt ttggattggg gctttgctcc cttgagcatc
    11041 ttccaccatt tgaatgattt gcgctaatac tgtctgcttg ccgacttgtg taacttccat
    11101 gtgaagtaga ccattcgtat tgattgtccc gccaaagagc gtgtcatccg gattcttttc
    11161 tacaggaaga ttttcaccag taagcatact ttcatctatc gcactgcttc ctgaaataat
    11221 tcgaccatct gctggaatct gctcaccagg acggatgact agttgatcac caacgactac
    11281 ttcttcaatc gctaacgttt cttctttccc gtttcttatc acttgggcgg tttttgtttg
    11341 aagagacatc aattgtttga ttgcattacc tgttttcgtt tttgctgtat gttccaaata
    11401 tttgccgagc aaaatcagcg tgatgatcat actgctgctt tcaaaataaa gatcttgggg
    11461 gtgtccacgg aaaaatccgt tgtaaatgct caatgcaaag gcagccgatg ttccaattgc
    11521 taccaataca tccatatttg gtgctttcgt cttgattgca tggtacgcac ctttataaaa
    11581 acgagcaccg acataaaatt ggacaggagc agacagcact aactggacga ttggaaaatg
    11641 aaaaaagtga acaatcgccg catggcttcc taacatcata gcaatcattg acaacatcaa
    11701 cggcaaagtc aaaaccgtac tcaatataag atcgaatagc atttttttca aatacgcttg
    11761 cttttcttct gcaatttttt gtttgtgggc ttcatcgtac aaaattgcac catagccgat
    11821 gttttctacg ctttggatta atttttcagt ggtcgtccct tcatatttga cacttgcttt
    11881 ttcagtggcc aagttgactg tggcattgac cacaccaggt tgtccgttca gctctttttc
    11941 gatcctagca gaacaatttg cacaagtcat acctgtaatg acgaacgtat ccattttggt
    12001 ttctgctgtt gccatcaaat cacctctgct ttataaccca atccattgat tgcttgacaa
    12061 atcttttcgg attgaacttt tgtttcatca aacttgaccg taccgtttgc ttttttcaaa
    12121 tttactttca ctttttgcac gccgtcaagt gcgcttactg tttcttctac tcttgctgca
    12181 caatgattgc agctcatacc ttcgattgaa aattgttgtt tcattttttc ttcctcctat
    12241 tgatgttgtt tacattcaca ttgacctggg atacagttac attcgatcgt atctactggt
    12301 tgcttttgtt ccagttgttt caaaatctca ttgatatctt cttgagtcaa agtggcttct
    12361 tctactagat ccgcaatcgt ctctccaatc ctttttgcac agatatggga aaaaagattt
    12421 tccgtcgcac tgcgaactgt ttccatctcc gagacagctg gatgataaat gaattttttt
    12481 ccttcttgtt ctgtccagat cacttctttt tttaccaaac gacccagcaa agttttgatc
    12541 gttgctggtt tccaatccat cgaatcagct aagatctgcg tgatttcttg ggctgtcgtc
    12601 tgtccaagtg tccaaataac acgcatgatc tcccactctg aatcactgat ctttactggc
    12661 gttttgactt tttccattta tttcacacct ttcgtttaca aatgtaatca ttatcttatg
    12721 aatacagatt acacttgtaa acaaaaactg tcaattattt ttgctctcta ttttcaattt
    12781 agttaaaaat tcttgtttct ctcgtcttgt tttaaggatg atcagtttat ttcttgcatt
    12841 agctacttgg atcagctgct gtattttcgg ttcattgttt tcttcaaaat gccagataaa
    12901 agataaaaat tctaagtatt ccctgtttag tttctccgaa aatgattcag gcatatctgg
    12961 ccgagatttt ttgtttatct ttctttttag agaacgccaa actactcggt agatggcgag
    13021 ataacgcggc actttaagcc aaatgatctt atctgcttct ttaatccttt cctctaatgt
    13081 agaagtataa tttccttcaa cgatccatga cggattagat accataaaca attgctgttc
    13141 ttgcaaaaac cacttttttg cctgaatact gtagtctgtt gtatgccata aaaggtctag
    13201 cggcagtagc ggaaagtcgg tgatttcttt gatcttcctt gcgagggtcg attttcccga
    13261 cccactttgt ccaatcacga tgatcttcat tcttctccct cacttctctt tggaatttgg
    13321 ttcgatcata ccacttataa cgccgcaaaa aaacaaggga ctgtgacaaa agtcgtgtca
    13381 aagtcccttg tttcgaataa atggtgttca atcagctgac cttcagcatt agctcaaaaa
    13441 gcggcaaaat atgaggagct atttttccgt ttgtttctct aatactcagg tcaaatcgct
    13501 gattttacaa cctcttcctt aaacggtgtt ctcctgccaa cacctcggtc acaagtcaca
    13561 atagtcaaca aacatgagaa gttgtttttt gaaattgctc cttgtgactc ggagttaaac
    13621 ggcaggctca ctacctcttt cttaaacggt gttccaaagc ttgcatctgc ggtaataagc
    13681 ccagccaaac gaaaaatatg aggggctatt tttgtttggc tgttcttatt acttgatgca
    13741 ggacagcttt ttcacaacct ctgattcacg gtgttccaga agtagcttct tcgaaaaaca
    13801 agccgaaact tcacaaaaat ttgagaagca attttcgtaa attccttctt atttcttgaa
    13861 gctgaccact tctgtcacaa cctcttgtaa caagtatttc tattttaagg ctgaaccact
    13921 gtttctggtt cagtgatttc tttgtttttg tctcgacgag caattgctgg caagatcata
    13981 cctaataaga tcaagacgat tggtgtgatg atattcgtaa ataattggaa ccaccatgct
    14041 ttaggatcag cagtataatc tacttttggc accattccta ggatacatgc aaacgcagtg
    14101 aaggcaaagc accaagcacc aaagataaaa ccgatttttg gatttttaac aaatttatat
    14161 tcagaattga atttcttata agctttattc aacatcatat atgccaagaa cacccatagg
    14221 taacgcattg gcatgacaac tgagttcaag ttcgtcaacc attttactaa ttcattcata
    14281 tctttgatac caaaaatcgg caacaaaata atgattccta ctagtatacc tgtcagaaga
    14341 taaccattca ccaatgtacc tttacgatta cgtttacgta gccagtttgg tacaaattct
    14401 ggatccgcat ctgctaacaa gatttttagt ggtgcatcaa ttgaaaaggc taatgcggag
    14461 atttgtccaa tcatgtttgt caatgcgtaa atgatcatca atgagttacc gataccatag
    14521 taattaccta aaatttgaaa agcactgtat gccccatttg ccatcaagtc tttaggaata
    14581 ttatcactag caaacagcat acccattgcg actgagccta atacagcaca aatccctacc
    14641 attgctgcta agaagatcat ccctttagga aattcctttg caggatttct tgtagagttg
    14701 acataagggg atattttttc cgcccctcca actgcaaata ctagcatcga aacggttgtg
    14761 aaataactga aatcaaatgt tggaatatat gttttcactt gattcatatg tggtgtggca
    14821 aattccatcg ttggattgat gaacggtgcc gttacagcta aaagaataaa taagatcgac
    14881 atgacgaaca tcgctgttcc ggccagtccg ccaatcactt tcaaagtaga aagtcctttt
    14941 gttgataaat ataaaaaggc aagaaatata ataagtgaaa taactgctac catttgtact
    15001 gacatcgtat ttacgatatt tccatttcct tggactgccc aaccaccagc aatcagaatt
    15061 gcttgaggct tttgtgccaa ataaggaata tggacgaccc agtaagtcca agcggcatag
    15121 tatgccagtc gtttattaga tgtcgtattt tcgacccatg aactgacgcc tcctttacta
    15181 tccttgaagg ctgatcctaa ttgtccaacg atcaatgcgt atggaataaa ataaatggcc
    15241 aaaatcaata gccaagaggt gacaactgat actccttgct gagcatagtt attaactaca
    15301 ttccccatgc cccaaaccat gttaaatgca attaacgcaa cagtaaacca gcgtaattgt
    15361 ttgttgttca ttcgttctct cctttatgat aaaaatttgt tatccagatg taatcatatt
    15421 caatttttaa caaacttaca agacagtgtt attttatgtg tgtattcttt tatttaatac
    15481 acatcgtttt ttcatatttt ttccatcaaa aaaataagtt attttctttt atttcattga
    15541 ccttattgaa tttcaagcta ttttgaattt tctgcaaaat taaaaacatt agttatttta
    15601 taatcctatc tattttcttt acatttttca ttaagaaaag gatataaaaa aaagaggacg
    15661 ttcattcgtc ctcttcattg atcaacgttt attttttaca tgcaagcttc gcatggcatt
    15721 caaaacagct aatactgtca ccccaacatc tgcaaatact gcatctccca ttgatgcaaa
    15781 accaaaagca cctaaggcaa gtaccaaaat ttttatccca atagcaaaga caatgttttg
    15841 tttgacgatt ttcaatgttt tctttgctaa atggattgct tctgcaatct tcgatggttg
    15901 atcattcatg atgactacat ctgctgcttc aattgcagca tcgcttccca atccacccat
    15961 tgcgattcct acatctgctc ttgctagcac cggtgcatcg ttgattccat ctccgacaaa
    16021 tgcggtcttt ttattgtttc tttgtaagat ttcctctaac cgctgcactt tatcttcagg
    16081 aagcaattcg ctatatatct tgtccacgcc tacttgcttc ccgatatgat cagcaatttt
    16141 tttcgagtcg cccgtcaaca tgactgtgtt cttcacacca gcctgtttta attgaaccaa
    16201 tgcgtctacg gcatcttctt tcaaggtatc tgcaataaca agataaccac tgtaactttg
    16261 atcgatcgct agataaagca atgtaccgat ttcttgactt aaaggaaaag agatttcaaa
    16321 tctttccatc aatttgtgat tacctgctaa tacttgtttg ccatcaactt cagctaagat
    16381 cccttcacct gcaaattcct ggatattagt tgttggtaaa agcggacctg cgtagccttc
    16441 taaaacagat tgagcaatcg gatgtgtaga aaactgttct gcactcgcaa tatattgcat
    16501 gaatttcttt ggatcgattt ttgtatcgat cgtctggatc gaaaagtcac ctttcgttaa
    16561 tgtgccggtt ttatcgaata ccactgtctc ggtttgcgca agcaactcca agtaatttga
    16621 accttttact aatacaccga ttttacttgc gcctccgata ccgccaaaga aactaagtgg
    16681 cacggaaatc actaatgcac atggacatga gatcactaaa aatgtcaatg ctcgatagat
    16741 ccattcagaa aacgccgctc cagaaacaac aagtggaggt aaaacagcta ataaaacggc
    16801 caatcctaca acgactggtg tgtaatagcg cgcgaattta gtaatgaaat tttcagctgg
    16861 tgctttttta ctgctggcat tttctactaa ttctaataat ttgctgatag tagaattttc
    16921 ataagatttt tctacttgaa ctaaaaatgg tttattttta ttgataaatc cgcttaatac
    16981 tgtttcacca acatatacag atttagggac tgattctcct gtaagtgctg acgtatcgac
    17041 taatgattct ccttcgatga tcgttccatc taacggtaca cgttcaccag gcttcacaag
    17101 tacaaactga ccgatctgca cgtcttcagg gttcacggtc atcaatcctt ccgtcgtctg
    17161 cacgtgagct gtttctggac ggatcgccat caattcgcga atcgattttc ttgaatgact
    17221 gactgccaat ccttggaaat actcgccgat ttgataaaat agcatcactg ctacggcttc
    17281 tggatattca ccgatcaaca tagcgccaat cgtagcaaca gacataagaa aattctcatc
    17341 aaagattttt cctttgacga tattcatcac tgccttcttc actacatcat atcctatcag
    17401 cagataaatc aataaatacc ctatcaagcg aattggttca ttaaaatcaa ataatagcaa
    17461 taaagcgata cctgcaaggg aaaataaaat ccggaacagc gtatttctct ctttttcatt
    17521 tccatgttca gatacttttt ccttatctat ttctgttact ttgacatctg gttctaattc
    17581 cttcactact ttttttgttt cttgttcaat cgtagccaaa tgatcttcct ctgcgtcaat
    17641 cgttagtttt ccagaagtga agtttaccgt tgcttccttg actccattga tcttctgaac
    17701 tcctttttcg attttcattg cacagttcgc acaatctaaa ccttctaatc tgtaactttt
    17761 cattttaatg acctcctcgc ctcaacatat gaatatctct tcataactaa ttataaatga
    17821 atggttattc atatgtcaag gtatccgaaa gaaaaaatga aaaacatctg catcggaaaa
    17881 tttcttccat acagatgttt tggcttattc tttctttttc tcttcgatca ttgcccctgc
    17941 tcttatccaa cccgcacttg ccttcttgat cttgacagga tagcaactaa gtttgaagcc
    18001 tgttgctggc aatttgtcta aattcattaa tttttcgata ggacaatatc ttttttcaat
    18061 ccctgcaaaa tgtccttccc agatattcga agctccacta cgctttcaaa aatatagaaa
    18121 gagttcttta atgaaaatta tttcaacttt ccataaacag taatcccata taaataaaag
    18181 acgtaaaagt cacatgggag aacttataca ggaaaacaca tagagtcagg tgaaatacat
    18241 gtatcccacc tgactctatg cgttttctaa cctttattat tctcttttta tacaatttct
    18301 gcaccgaatg gcatcaacgc caatcgagca agtttgaaag attgcttggc aaaaggtatc
    18361 cctatgatcg tgatacatag aaggatgccg ctgacgacat gtgcgagagc aattggtagg
    18421 cctgaaacga tcagccagat gatattaacg atcaatgaca tgccagactg gtgataaatt
    18481 acttctttgc caaaaggcgc aaaagaaagg gaggcgatct taaaacattg cataccgatt
    18541 gggataccta tgatcgtgat acaccaaagt atccccgctg ctaaccagct aagaccaccg
    18601 attagtccgc cgaatataaa ccagatgaca tttcctaaac aactcattgt atttctcctt
    18661 ctattactgt ttttgattaa aactgggcaa aaattgattt tattcttctt tttttagctt
    18721 ccagcatcaa gtattctgca aatttttttt ggatttcttc cgtattttct tttgttacta
    18781 atgtgtgttg gattagtttt cctgatggtc gctgatccac ccgttcttct gactgttgca
    18841 cgtccgcttg cgcaaatccg ataatccgtt tttcttttgt tacagcaacg agcaatagtt
    18901 tattcttatc atttaggtca gctgccgttt ttgcttttac gaatgattct ggatgatcag
    18961 taatcagctg attgatttga ggtaaatctt cttttactgc ccaccgtatt tccatcattt
    19021 ctcacttttc tttcgctcat ttttatctcc actaaaaaaa tcgactgcgg caattgcatc
    19081 tatccagctt tgtgcaatga tctcatgtcc gccaatcgtt gggtggaccc catcttctct
    19141 tgtatatttc gaaaaaccat aagcaatccc taaagcattg aatatgccat ctaaaggaac
    19201 cagcaatgcg ccatattcct tagcaagttt acgaacaatt tggattttag ggtctaagtc
    19261 tttacgccag ccatgacggt cgtcggttat aggaagtaca aacggctcca tcaagatgat
    19321 gttgcagatt tccgctgttt gaagttcatt tagtgatttt cgataaacgg attcaaattg
    19381 ctgcgcagat ttctcagttc cgaatatagc ctgatcgcta gtattatgcc aggtatcatt
    19441 gattccgatc aaaatcgata catagtcagg ttgtaaagct aaacaatctt cttgccagcg
    19501 tacttgcaga tctgcaattt tgtctcctcc tatcccacgg ttcatgcatt caaattccat
    19561 cgttggatat tgggagatta gttttccggc aatcaaaagt ggataacctt tacctagatc
    19621 agttgcttct gctcggtctc ttcctgcgtc tgtaatgctg tcaccgataa ataatatacg
    19681 ttttttcatt tgtcaaaaac tcctttttga ataaaacgat gattttcttc cttatattat
    19741 cccataagcc ctatcaagac gaaagagaaa atatatgtat aaaaaatgac accagcctag
    19801 tcccttcaat caattgaatg aacgctcatc aagtgtctag ttttttatac tttttttctt
    19861 aacgtgccgg attccccttg attccttcgt tgtttggtcc agagaaatcg atcacaccta
    19921 agcttccgtc aatcagacca ctgatgacgc catcagaaca tatatttata ggctcatcta
    19981 agacttccag taaatgttct tttactaata tctcttctag attgcttggg atagattggt
    20041 cataacttta tctggtccga tgatttcaag gatgaattaa acttacatat ggatcattgc
    20101 atgctgaaaa tacggaccta ctccatcttc gaatgttact attagtttgt tttcaggtac
    20161 tagatgctgc tttatttcaa cagcagaagc atcatcaaat cagatttcca tttgaataac
    20221 cttctttgta tatgtttgat tgattgcgat ttcgttatag aaccttgtga atcaaatagc
    20281 aatttctttt gctttcttct cttgtctgct ggaaacttcc tttatcccgt attttaaaaa
    20341 aagaactgcg agagttcgca gttctttttt ttaaataaac gttgctctcc ggccaactcc
    20401 tcggtcacaa gtcacaatag tcatcaaaca tgaaaaactg ttttttgaaa ttactccttg
    20461 tgactcggag ctgaacggca ggcgcacaac ctcttgatat aaacggtgtt caatcagctg
    20521 accctcgata ttagctaaaa aaacggtaaa atatgagaag ctatttttcc gttttgtttc
    20581 tctaatactc aggtcaaggc actgatttca caacctcttc cttaaacggt gttcaaaagc
    20641 cagtttctcg gtattaagcc aaatttatgc aaaaatgtgg aaagccattt ccacaaaatt
    20701 tttcttaata ctcgaaacta aacagctttt tcatcacctc ttatggataa attcggttca
    20761 acaaacgtgg gaatggaata gcttcacgta catggtcgat accagcaatc caagtgactg
    20821 ttcgttcaag gcctagacca aaaccagagt gcggaacaga accgtattta cgtagatcaa
    20881 gataccatga atattctttt tcatccaaac cgtgattacg gatttgttct agcaagaaat
    20941 catagtctgt tgcccgttcg cttccgccaa taatttcacc gtaaccttct ggtgcgatca
    21001 tatctgcaca aattaccacg tcttctcttg ttggatgtgg tttcatgtag aatggcttga
    21061 tggcttttgg atagtttagg ataaagacag gctgttcgaa tgaattcgca atgaacgttt
    21121 catgaggtga accaaagtct tcgccccatt cgatgtcatc aaatccattt ttcttcagta
    21181 attcgattgc atcatcgtaa gtaatacgag ggaatggcaa ttttgtataa gtttttaaga
    21241 cttctttgtc tcgtcctagt acatctaatg catattcgca atgatctaaa acattttgta
    21301 ctaagaatgc tacatattgt tcctgtactt ctaaactttc ttcttgatga gtgaaagcca
    21361 tttctggttc gatcatccag aattcgatca aatgacgtcg tgtttttgat ttttcagcac
    21421 ggaaagtcgg accgaatgaa aagacttttc cgaaagccat agcagcagct tccatgtata
    21481 attggccact ttgagataaa taagcttttt gatcaaagta atttgtttcg aatagatccg
    21541 tagtgccttc tggtgcagaa ccagtcaaga tcggcggatc gattttgaca aaatgatgtt
    21601 gattaaagaa ttcatacgtt gcacgaatga tttcatttct gatctgcata atcgcatgtt
    21661 gtcttgaaga acgcagccat aaatgacgat gatccatcaa gaaatctgtt ccgtgttctt
    21721 ttggtgtgat cggataatcg tggctctcgc ttaccacttc cacatttgta attccgattt
    21781 cataaccaaa ttttgaacga ctgtcttcac ggatttctcc agttactaac acggatgttt
    21841 cttggtgtag atttttagct gtttggaaaa cttcttccga cacttcgctt tttacaacta
    21901 caccttggaa aaaggctgtt ccgtctctta attgtaaaaa tgcgattttt ccgcttgatc
    21961 gcttatttgc gacccaagcg ccgattgtta ctgtttctcc cacatggttt ttcgaatcga
    22021 tgatttgaat ttgttccacg tatgactctc cttttatatt ccttgaattt cttcaggatt
    22081 aattttgact tttactctca ataaattgct tgattctgcg taccgcttct tctaacgtag
    22141 cgcgatcagt ggcataactc atgcggacat tttctggtgc cccaaagcct tcgccagtca
    22201 caagcgctac ccctgtttct tctaaaagat cttctaccca ttgtgtgaca ttttcatact
    22261 tacacatgcg tagcgtttct tttacattag gaaaaagata gaaagctcct tgcggctttt
    22321 caagagaaac acccggcagt tctgccacaa gcggatataa cgtattcagg cgttcttcaa
    22381 aggcgttccg catttcttct actgtatctt gctcaccttt caatgcttca acagctgcat
    22441 attgactgac tgctgtcgga ttgctagttg attgggaagc aacagcgatc attccattga
    22501 tgatcacagg atttcccacc gcataaccaa tgcgccagcc tgtcatcgca tatgttttag
    22561 acacgccgtt gatgatgatg gtctgatttt tgattgcttc tgaaatcgtt gcaattggag
    22621 taaactcatt gccattataa actaaacggc cataaatatc atcagcaact atcaaaatat
    22681 cattttttac tgcccattca ccgattgcct gcagttcatt cttgctataa atcattccag
    22741 tcggattcga tggagaattg atgattacgg cttttgtttt ttctgaacgt gcttgttcta
    22801 gctgttcaac agtaactttc cagctattgg attcttcccc tttcacaaag acaggaagtc
    22861 cttctgccag ttttacttgc tcaccgtaac tgacccagta aggcacagga atgatgactt
    22921 catcttgcgg gtcaaggatc gtttggaaaa gggtatacaa agcaaatttc gctccgtctg
    22981 ttacgactgt ttgtgaaggt tcatagacaa gtccatagtt tttcttcaga taatcaacga
    23041 ttgcctgtcg aagctctggg attcctgctg ttggtgtata atagctagct ttcccattgc
    23101 ggattgcttc gattgctgct tcttggatgt tttctggtgt cgcaaaatcc ggttctccca
    23161 cagtcaaact cagcacatct ctgccttttg cttttaatgc cttggccttt gccgcagctg
    23221 ccaaagtgac agacggttct aatcgttttg ttctttttga taactccatc ccattcattc
    23281 ctttaccttt taaattccct tgattgtctt gacttcatct ccagattcat agccaatcag
    23341 gtaataattc agtttcccat ttgtttcttt tcctactact tcccacacag gctgatcatc
    23401 atacatccct aaagcagctt tttcgatttg gatttcagga tgggccgcag caattgtctg
    23461 ttttgcttca ttttcagtca gtccctttgt ttgatccaat acttttactt tttcaccaga
    23521 tttaggaatg ataacggcga tttcttttcc tttatcattt gtacctgtca gtgaaaagta
    23581 tgtcttcttt cgagtaaacc aataaaaatg atcggcttcc tcaagatttg catatttctt
    23641 tgcgatttct actgcttctt tcttcgcctg caccgccgga cgactggaac gcaagtaaaa
    23701 aatcgagctg atcacgatga ccgctaataa aaatacgatc aagccaagta acacgcgatt
    23761 aatcgttttt tcctttgtct gttttttctt caagggtcgt cgctccttta tcttcattcc
    23821 ttttcattat agcagatttt aattatgaaa aaagacagct cctctaactt ccttcttcat
    23881 ttttctttaa gaattcatgg atatcctgat agatttcctc catagctagc tctttgatcg
    23941 gtactttttt cggtaatgca tggatgatcc gtgcaccata ttttgcagta actagtcttc
    24001 gatccaaaat aagcaatacg ccacgatctg tctccgtcct tatcaagcgt cctaatgctt
    24061 ggcgtaactt caaagttgct ttaggtacgg attcctggta aaaaggattg atcccttctg
    24121 attctaaata agcgttccta gctttgacca tcgggcgaag cggattatcg aagggtaaac
    24181 gtgtgactac aacgatctgc aaagcatctc caggcaaatc tacaccttcc caaaaactgt
    24241 cagcgccaaa caatacgctt ttttctgaca aaatgaagcg tttcaaaagc ttctcccggc
    24301 ttccgccaaa cccttgagct agtatttctc gtcctttttc tagcatagac agatgcattt
    24361 tctgatagac cttctgcaaa acatcatgag aagtgaataa cacaagtatt ggccgatctt
    24421 cttgttcgat cagacctgta agtgctgtct ctaaataatc gatatatgct tgattgctga
    24481 cttgatggac ggcaggtgcc tcttttggca catagatcct tgcctgtttc tcataatcat
    24541 aaggagaggc aatcacttta agaggtgctg cttcaatgcc aagcctctta gagagatagt
    24601 gtcgattagg gccgatcttc agcgttcctc caatataaag aatacgatca tatcgaggat
    24661 accaaacagt cgacggcaac atagccgctt gaaaatcatg gagatgaaaa atcatctgct
    24721 gcgctgtgtt cccaggaacg aaccaatgga tcaaatgatc tcgccagtct tccagccatt
    24781 gatccatcag caagtactgc ttgtctaatt gatcaaatag gctatataaa cttgctaaat
    24841 cgatccgcat ctggtttgtc cagctttctt ggataatctc aaaagtcaga cgcatttgtg
    24901 actgtagctg ctggatctcc tgataaaaaa gctgcaggtg atcaatggct gtcccagatt
    24961 ccagtggcag ctgatccaac atctcttttg tcatcatgac ttcttctgga tactgtccgt
    25021 tgggttgctt gccgctcact aaactcatta atccttctgc catacaaatc tgtgcttcgc
    25081 atagcgcaga aagttcttct cgataaatcg ataaccagcg taaggcttca tgttctttcg
    25141 gtaaatagct ttgtattcgg tcaaacagct gatttggttc ttctaattgc tggatttgtt
    25201 tatggaaata gttaatactt aatcgtttgt catttacttt ttcagcgatt tccggcaaat
    25261 gatgtacttc atcgatcaac aaaaatggac tagctggaag caatggttct tttcgctgtg
    25321 tttcttgtac taaaaacgca tgattgacaa tcaaaaaatt actttgcctg attttctttt
    25381 gcaagaaacg aacaaagtct tgttcgtaaa atgactgatt tcttgctaaa aatccaatac
    25441 ctctgtgcgc gatatctgca aagaatgaat ggttcaaatt tgttaaatgt aattcatcca
    25501 aatcaccggt cttcgtttgc gtcagccaaa ctaagaccgc catttgatac aaagcatatt
    25561 gtttttgttc tggcggttcc acaagtgttc ctttaaatcg ttgcaggtcg atatagtggc
    25621 gatagctttt gataattaca ggttgtatgg gctgatctag caattgattc agcaatggaa
    25681 tgtctttttc tatgatctgc tgctgcaaaa ctagcgaaac ggtactgatg acagcgggtt
    25741 tttcaggtgt agccaaataa ctcaatggta gcagataacc gattgtttta cccattcctg
    25801 tagctgcttc gatcatcaaa tcttttgatt catcttttgt gaaatggtcg tagaccgcat
    25861 tcatcagtct gttctgttct tttcgataca tcagtgtttt accaaacatt ttttcttttg
    25921 cttgtttttt tcttggatat gtccgctcgc cataatggac ggatgtgaat agttccactt
    25981 cttttttctg taaagctagc ccatcgacga tttctaacga gggatcaagt ggctctggtt
    26041 tttctttcat ttcttcaatc acatgataaa tgaaacgact cgtatccata cctgtgtgtc
    26101 tacttaatcg cgcaatctgc tctaacgtga tgattggcaa ttttctcatc cgttcttcga
    26161 tcagcaaaag aagttgacct gtcacttcgg catcactatc tgcctgatga ggattctcat
    26221 gaataaatcc aaggctttct gacaaatcag ctaagcgaaa gcttggttcc gtcggcaaga
    26281 aaatttgtgc aagttctact gtatcgatgc ctggtatctt caaactagga agtccgcatc
    26341 gcatgagttc atggttcaaa aaattataat caaaataaat attatgcgcg acaaatatgg
    26401 tgtctgccaa aagattgtaa atcgtttggg cgacatcttc gaaataaggc gctttttgga
    26461 cgcgttgatt cgtgatccct gttaaatgct ggatttgttt tgaaatcttc cgccctggat
    26521 gaatatcgat cgaaaaacgt gaagtgatcc gacctgattc aatcatgacg cagccaaatt
    26581 ggatgatccg atcttctttt ggattcgtcc cagtcgtttc gatatcaacg actgcataag
    26641 ttcttggttt caaaatatct tcctcttctc ctgcctctaa tgttcttttg aaattatact
    26701 acaaagtgca gaaaaatact ctcatcgctt tttaaagaaa atagacaaaa acgcccagac
    26761 acgcaaatgt ctgggcgttt attttttagt ataggttaat taagctttag taacgtttgt
    26821 tgcttgaggt ccgcgttggc cttcttcaac atcgaaagtt actgcttggc cttcttctaa
    26881 agttttgaat ccgtcgcctt ggatagctga gaaatgaacg aatacatcgt ttccgttttc
    26941 tgcagtgatg aatccaaatc ctttttctga gttaaaccat tttactgtac ctgtttccat
    27001 aataaaaatc ctccttgtgt atcaacacat attttgcaat tacttgaatg gtgtatatct
    27061 ggaagatgtt tatttgaaac aagctaccac agattaccga acaaaaacta ctcctctagt
    27121 atatcatacc gtttacaaaa aagctagagt ttttcgactt tttttattaa aaaaagtttt
    27181 ctcagcgatt acataaagcg aaaaaccgaa ctttttttaa aataaacgac atattgttca
    27241 gttggtgttt ttttgctctg atgttctttt ttaaactaaa aaaattattc tttctgacta
    27301 cttatcggtt ctgtttcaac ggtcgaggct ggggaagatt ctttagtagg tctgatgttc
    27361 tcatggtatt tctgatagcc tatgacagtt accagaagaa caaggcaaac caatacgatc
    27421 aacgagatta gtttttcttt tttgctcata tctatacctc ataaataaca ggatgatgtc
    27481 catcatattt ctgagtatga gtaaaagcaa aagctgcagc tgtagaatgt aagatatgcc
    27541 agcaactttc ataatcacag gtcggacagt accaaaagta accaaatctt ggctgcaaag
    27601 accgttcttc tgtttcagga atcgcagaat caagatctgt tgtctgtaca taaacatttt
    27661 tgtacagacg tgtgcctgta ataggagacc gctgtgtttc ttcagcaaaa actggctgac
    27721 ttgttaaact tagggataga aacagcaaac accataacag tctttttttc ataaagacct
    27781 ccttatcgat aagtttttcc caattaaaat catatctcaa gaacgcccgt tattcacatt
    27841 ttgttcataa atgatgtttt ttgtatcata atcgtcaaaa agaggtcgtg accacgtcac
    27901 aacctctttt ttaaacggtg ttccagaagt agcttcttcg gaaataagcc gaaatttcac
    27961 aaaaatttga gaaataattt ttgtcaatct cttcttattt cttgaagctg accacttctg
    28021 tcacaacctc ttccttaaac ggtgttcaaa agcttgcatc tgcggtaata agcccaccaa
    28081 acaaaaaata tgaagagcta tttttgcttg ctgttcttat tacttgatgc agaacagctt
    28141 tttcacaacc tctcatacac ggtgtttaat cagctgacct tcggtattag ctcaaaaaac
    28201 ggaaaaatat gtgaaactat ttttccgttt gtttctctaa tactcaggtc aaaacactga
    28261 tttcacaacc tcttttaata acgaagaatc gctgtcagac tcttttcatc tggcgcatct
    28321 ttttgatcca aaataaacac tttcccgcca ttaatgatca catcatctgc taatgcattc
    28381 aacacttgac gccgatcata ttctgtttca ggatcttcac cgaaaccatc agctaagttt
    28441 gacgtagcga taaataaatg tgacactttt ccttcttttg cagctggaac gatatcaact
    28501 aactgatcga tgaatttctt atctaagagt ttattgtatt cttttgattc ttgagctgcg
    28561 agttcacttg acagcttctt cgccccttct tcgatatctc taaaggatag ctgcgcagga
    28621 gaagcagaaa tcgcaacgtc tgcacaatag taaggatttt tggcgatttt tttgaacatc
    28681 gtctgatttt ctggtagagc aaataaataa atcggccatt gttctggatt ttcgaaagaa
    28741 tccttcaagt acgtatcaac tgcttggtaa tagttgaccc aatcgatctc tacttcttca
    28801 tcttttggat ttacgccatg gaaagttact ccatctcggg ctccgcctcc tccattagaa
    28861 gagaagttca aaccgccacc tgtcagttca gatcccaatg cagtaacgac atcaaccggt
    28921 gccccttctg gtagagatac ttcttttact tgtttgttgc tgacttcata caatttcatg
    28981 gagtcgcggt tcaatcccaa taaataataa gaatagttga actgcccatt tttgataata
    29041 gccagtaaat aaggaagatc acttacatag tattgatcgt ctaccgggat actcaaacga
    29101 tggatatagg tttggtcagc agttataatg accgctacac tcgtagttgc actacgccaa
    29161 aacgaaggat ctcctaacaa attttcaatt ttttcttgga acggggacca gtcatgttcg
    29221 gaatattttt tctcaaaacg tttctttgct tctttcgaaa aatttttgaa tttgatctga
    29281 tctttttcca catcttgatg ggcgacatgc gtattcagca tgatcgtgac gaatggtcct
    29341 ttagcgcctt ctgaaatcaa ttgtgacaat aattctttat ccttttttgc cataaacgta
    29401 cccctcctaa aaaaatcatt cctttgtttc tatctaaagt ctaccacaac actatttttc
    29461 catacaataa aacgtatcta aaaaaataaa acggtttcta cggttttaaa tttagctatt
    29521 tttttaagtt atcttcataa tttcctcaat tatttagccc cagtcttcaa ctattcttca
    29581 tgaaatgctg ataaactaag aaaaagaata aaagaggtgt atcttatgca tttgattatg
    29641 attttcagtt tattaagtat tatcagttta tttgctttca gcgcttcccg cttattagag
    29701 gcggaaacac agcctgttcc tataaaagta cgaaaaaatg accgctaacc ttttcataac
    29761 gcgcgttctt atcctaaagt ttttttcaga aacattgaaa ctctcttcct tctctgataa
    29821 aattttaatt tatagcgaaa caaattaaat acaaggagat cggatatgtc cataaaaaaa
    29881 acacgtctag tcctattatt ggctagtctc attctttgcc ttgcttcttt tctaccagct
    29941 gttgctgtgc aagctgaaga tacgttcaaa gtaaatgcaa aagcagcttt tgctgtggat
    30001 gcgcaaagcg gtaagattct ttatgatcaa gatggagaaa aaaccatggg gatcgcttca
    30061 atcacaaaaa tcatcggtct ttacatcgtc cttgatcaag taaaagaagg aaaactatca
    30121 tgggatgata aagtttcgat ttcagattat gcagaaaatt tgagtatcac cccagattta
    30181 tcgaatgttc ctttgcataa agaaaacacc tacactgtca aagaattatt cgactctgca
    30241 atcatccagt cagccaatgc ttcaatggtg gccttggcag aaaaagtctc tggcagcgaa
    30301 gctaaattca ctgaacgaat gaaagaacaa ttgaaagatt ggggaatcaa agatgcaacg
    30361 atcgttaatg catcaggatt gaacaactct tatcttggcg aaaaccgacc agaaggcact
    30421 ggagaaaacg acgaaaatca aatgtctgct caagatgtgg ccatcgttgc ccgccatttg
    30481 atcttggatt tcccagaaat actggatgta tcaagtacta cgacccaaat gtttggcgaa
    30541 aatacgcaat caccagtgga aatggtcaac tggaactgga tgctcccagg attcgttaat
    30601 tacaaggaag gcgtggacgg attaaaaact ggtacgactg agcttgcagg tgcttgtttc
    30661 gttggtacga tcactaaaaa cgatcaacgg atcatcactg tcgtattgaa tgcggacggc
    30721 catgcggaaa acccgagtgc ccgcttcaat gaaacagacc gtttgatgga ctactgctat
    30781 gacaattggt cagaaaaaga gctcggaaaa gctaacgcaa gtattcctag cctaaaaacc
    30841 attgatgtaa aagacggaaa agaatcaaca gttcctgttg tgcttaaaag ccctgtaaaa
    30901 gtatgggtac gaaacgatat ggatactggc aaattaacga tcacgccaac acttgacaag
    30961 aagcaagtca ctgacaacga gatccaagca cctatggata aaggtacaaa aatcggtacc
    31021 gctacgatca ccttggcaga cgataaatta ggttatttag aagataacaa tgcgccatcg
    31081 acagagatca ttacggataa aacagtggaa aaagcgaata tgttcgtgat tggctggcga
    31141 cgcgtcgcag aatttttcgg caacttgttt taattgaaca ataagaaaag catctttcca
    31201 tcggaaagat gtttttttgc tttttctagt ttaataacag cttttttcat ctattttcgt
    31261 tatactgtat aagaaaaagg agttgaattt atgaatctaa ccagagatct gatcttttta
    31321 gttctctatc ttattggggt attctctatc gtttattggc gaaaacagaa gaaggatcct
    31381 tccttgttct tttacggcag tacgtggaca tttacggtaa tcttaactta ttttgtcgtg
    31441 ttggaaattt tttattcgac cttttatttt ttgattccat tgttgttctt tagtatcttt
    31501 gctttttctt atttcacaga aaaacgaagg ctgctgaatg gtcttttatt caatgttttc
    31561 ctcatcagtt tcggcatcta cttgtttgtc ttgctgtatg aaacgcaaaa tattttcctt
    31621 ggcggactga tcgccttgat cacgattcca ttgctacttg tccttttatt tggtatttat
    31681 ggattgattg tttttctttt ttggaacggg gtcacagttt tacgtagaga atcgcattca
    31741 ttagctaatc ttttaacgtt gatactagcc atttttctta cactgtttct tgtttttgac
    31801 tttttcttgt taaaatattt gccgcaatgg ataaatgctt tgtttttctg cgttcccctg
    31861 atcttgatct atctatttat cgtattctac aactttttga ctgtctcatt cttgtatcag
    31921 ttcaaccggc ctcgatacaa tcaagacttc atcgttgtgt taggcgctgg gttgatcaat
    31981 ggagaaacag tttctcctct tctagccaag cgaatcaata aggcgattgc tttttatcgg
    32041 gctcaaagcc gtgctacatt aaatccgccc atcttattga tgtctggcgg gcaaggagca
    32101 gacgaaaaag taccagaggc cattgcgatg aaacaatatg ccatggaaca aggaatccct
    32161 gaaagagaca ttcttgtcga aaccaattcg acaaccactt tggagaacat gctctattca
    32221 aaggaaatca tggatcagca aatgaaaggg ccataccgcg tgatctttag ttccaacaat
    32281 taccacattt ttagagccgg tctttatgcg tgtcaagcca aattgaaagc aaatggtatt
    32341 ggtgcaaaaa cagctttcta ctacttgcct aatgcgtttt taagagaata catcgccatc
    32401 ttgatgatga acaaaaaaag acatatgatc atttgcggat tgcttttcgc aggctccgca
    32461 ttcttatcgc tgattacttt acttttctaa aaaaacaagg tgtgacaaaa catatgtcac
    32521 accttgtttt tttctttcat tctcttaact attggcaaaa agagtatccc ttttttgacc
    32581 gctacttgct gagcagaata aataccgctg ttgcctgaac acataaagct gatcaagcag
    32641 atcataaaga agtatactgc agcttgtgaa ccgaacaatt cgatccccat cacaaagcag
    32701 gcaatcggtg tatttgtcgc accagaaaat actccgataa agcccaatcc tgcaagaaaa
    32761 ggaacagaaa catggagcaa aggcgctagt gctgcaccaa gcgttgcacc aatttcaaac
    32821 aatggtgtca cttctccccc ttgatatccc gcgcctaaag acaaaactgt gaagaataac
    32881 ttcccaataa aatcatagac atgtgcctgt ccattaaatg attcttgaag taacggaaga
    32941 ctcaatccga gataacgttg tgtttgcaaa atcaatgctg ctgccacaac tacagctcca
    33001 cctatcgtat ttcgccaaac aggatgagga atccattgtg catatcgagc ttttaaaaag
    33061 acgatcgaac ggctgaatac ccagccaatc aaaccaaaag caatcccagc aacaaaaact
    33121 ttgaagaaca gcaacatact ccattcagga atcacaccca tttggtaatg tagatgagaa
    33181 acaccatagc tttctgtcat gaaatttgca aacagtgcag caaagaagct agggaagatc
    33241 gcttctgttc ggacttttcc gattgccaac acttctaatc caaataatgt tccagctaaa
    33301 ggtgtgccaa atacagaact aaaacccgca ctgattccac tgataatcaa gatttcccgt
    33361 tctgatttat ccaatcggaa caaacgtgct gtattatctg ctaacgctcc ccccatttgg
    33421 actgctgttc cttcacggcc aacagatccc ccaaataaat gggtagttat ggttccaaac
    33481 agtgtaagtg gaataagtcg caacgggatt ttttcttctc caccatttcc ttgatcgatc
    33541 actaagttgt ttccacgact agagagaccg ccgtgataca catagagata agcaaaaagc
    33601 gcaccactaa ctggtagtag ataaaggagc caaggatgtg atagtcgggt atctgtcaca
    33661 actgtcaagc tatttaagaa aaaagcagat aagctcccca tgaatatacc gagaatcgct
    33721 gtgatcacca gccacttcag catatacact aaaatgcgca ttggactcgg taattgtttt
    33781 tccataaaaa attcctcctc aataaaaaaa cgcctactaa agaaaggggc tgtccccaga
    33841 ctttagtagg cgtcattagc tatctttcgt tagcatttaa aggcgaacac catcacctat
    33901 tcacttccat taaaagatag catatttttc tgtaaaaaac aataaaatta tcgttctttc
    33961 tttacacgtt cagcaaattc atagaaagga gccaatttcg ttaactgttg attacattgg
    34021 tcactatcat gacaaatata attccccttt ttcgtatacg tgccatcaga acctgctttc
    34081 gttgtagaaa gaaaaagcga aacattcgat gttttctgac agattgcaca gattccctta
    34141 tttatcgttg gtgataaagt accactaatc ccttttaatt tttcatcttc gtaatagacc
    34201 atgaattttt tctgcgtacc tggatcattc cagccgatgt acgtatgttc ttgcaagtcg
    34261 atttcattcc aagctggttg tttgagtttt ttcacttttc ggaataattt ctcgatttgt
    34321 ggtttgctag gctgcataaa cggctgaacg atttgcttca gctcctccaa tgctctttct
    34381 gcttgcgctt ttgtcagttt tacgtctaaa actgtcgtta aaaatgtatc gatttctagc
    34441 agttcgtgat ctaatatgtc tcgaatcttt tcagctgcta gtgcctgaac tgtttgtacc
    34501 gtttttggat cattgacaga tgagtacgca tttaataact gttctacttc tctttttata
    34561 aaaaaatatt ggtatggttt tattgtttgt tccatgattt taaagtcctt tcttctaacc
    34621 aaagtgcata ctttgtagaa aaaagtgatc ttctgcaaag tatgtgttac ctgaagatcc
    34681 cggactcttt taaataagat acgttttgca tcttatcatc acccccttta atgcttattc
    34741 ttctatgtac atgattcatt cgataaataa aagtaaaccg tatcttgaac agatagtcaa
    34801 ctgcaaacag aaaaaaggac tgtgacaaaa gtcgtgtcaa agtcccttgt ttcgaatgaa
    34861 tggtgttcaa aagcttgcat ctgcggtaat aagccgaaac ttcacaaaaa tttgagaagt
    34921 aattttcgta aattccttct tatttcttga agctgaccac ttctgtcaca acctcaatat
    34981 ggtctattat tggttttacc tgttaaatcg aggattcttt cccaaagtaa cgttgccatc
    35041 tcttacgcat acttgcagac atgggctgag ccgcttcttt gactgcgcag tatttgtcca
    35101 cgaatgtcac gatatatcct tctttgtact taggcggtgc gatcgttgca ccccacatgt
    35161 gcttcaaaat gatatctcgc tcaagatccg acaaatcggt gattttttct gcattcttta
    35221 cagcaattct tggatggata tacgcatgtg ttccctcgtc aaatttcgtc gtacgccaat
    35281 catagtagaa cagatcatgc aaaagtccag cacgcgctgt tgctttagca ttgccacccc
    35341 atttttttgc gagcagataa ctatagtaag aaacactgat ggaatgctcc aaacgagtgg
    35401 aatgaacatg ctgtgtgtaa ttcgctaatt ttttcactgc ttctgtttct aaaagatctt
    35461 gtacataaga catatattct tcgtcttcac gccatttttc tgtcataatt atcgatctgc
    35521 cacctttttg aatttcattt tcttctgaaa ttgatctaag tataccacca gtgttcggat
    35581 tattctagtc gctctagata aaatagctaa agcttaatag ttccttaatc ataaagaaaa
    35641 atgataaaga acgatccctg ctgcaactcc gacattcaat gattccgcat ttcctttcat
    35701 tgggatatat acgttctgat cagtcaaaga caagatttcc tgacttactc cctgaccttc
    35761 gtttcccatg attatagcga aatcctctgt tttttctact ttgtctaatg catgtgcttc
    35821 ttcatttaat tctgtaccgt acaccgtaaa ccctttgcct ttcattacag ggattacatc
    35881 tgccaaatct ttttgtaaca caggcaaatg ataattgctt ccctgcatgc ttcgcagcac
    35941 ctttgtacta taaatatctg ccgttccttt acctaataaa acaccagcca taccaaacgc
    36001 atctgccgta cggatcatcg ttcccacatt tccaggatcc tgtacattat ctagtaaaag
    36061 ccagcctcct gaaaaatctg gcgtatcttc agatgttaac gtcatcactg ctaagattcc
    36121 ttgcggtgta ggtagatctg acatagcggt catgacctca tctgaaacta gaaaagtaga
    36181 aatctctttg tttacagcca gccagtctcc ccattctttc atacctctct gtgtcacaaa
    36241 aagttctttt atttctgcat cagacgacgc agcttcttcg atcagatgga acccttccaa
    36301 aagatattct gccttttctt ctctgtattt ctttttatgt agttttttta attctttgat
    36361 tcttggattt tttgctgatt ggatctcttt cacattcctc acctcaactg attatatcat
    36421 ggaataggtg cttgagcttg tttcatccta aaaaaatcgg tatcattgat ttataaaaca
    36481 ggaaagaaag gtgacgaaca tgcgcaaagt taaaatgaat gtccaaggac gtgtccaggg
    36541 cgtgggattt cgctatatga ccaagatggt tgctgatcag ttaggcgtga ccggaacggt
    36601 aaaaaacgaa gacgacggct cagtcacaat cgaagcaatc ggaaatgacg acatcatcca
    36661 aaaatttatc gaagaagtca agaagtctcc gagcccaagt ggacgagtgc agtatgtgga
    36721 tgtccaagaa gatccgatga ttgaggaacg aaagaaattt gatgtggtgg gataaggttg
    36781 tttttgaagc gctttgcctc gagtattaag gagaaacatt ttcaaaatag cttctcatat
    36841 tttgaaatgt aatgacttaa taccgagagg cagctgataa aacaccgtca ataaggttgc
    36901 gaaaaagctg atctgcatca agcattaagg actggttgcg aaaaagtcgc ttagttttga
    36961 gtattaagga aaattttgtg aaaatggctt tccacatttt tgcataaatt tgtcttaata
    37021 ccaagaaact gacttttgaa caccgtcgat aaggttgttt ttgaggcgct ttgcctcaag
    37081 tattaaagac aggtagtaaa aaagctgatc tatcacttgc agtgagttca tttgactcct
    37141 ctatttcggg cagaataaaa aagtttttag ttttttaaat aagaaagaaa cacttggaat
    37201 cactcttttc tttccaagta tttctttctt gattttttgt cttttttcaa aatttttcgc
    37261 tacgaaaaag aaagaatatt tatatgaaat ttctatgaag ttgattgaat tccatgcgct
    37321 tttttagtat agttaagttt gtgtaaaaat tatagaagag gaagtattct atgaataaat
    37381 ggaaaaactg gttattggga tccggacttg tcagtatcct gctcttttta tctggctgtg
    37441 tgcggatgga ttcaaacgga aaccctgaca cttcaggaat cgtttatcga gtactcgtcc
    37501 atcctatggg ccaagcgatt acatatctag ttgagaattt caattggtct tacggctggg
    37561 cagttattgc aatgactgtg atcgttcgta tcatcatctt gccactaggt atcagtcaat
    37621 cgaaaaaaac aatgatccaa tctgaaaaaa tgcaagcgtt aaaaccacaa gttgaagcag
    37681 cacaacaaaa gctaaaagct gcaacaacac gtgaagaaca aatggcagca caagcagaaa
    37741 tgcaacaagt atatcgcgaa aacggattga gtatgaccgg cggaatcggc tgtctgcctc
    37801 tattgatcca aatgccgatc ttctctgcct tgtattttac ggctcgctac acagaaggta
    37861 tccgtgaatc atctttttac ggcatcgatc taggctcacc aagtatggtg cttgttgcca
    37921 ttgcaggggt cgcttatctg cttcaaggat atatttcaac aatcggtatt cctgaagaac
    37981 agaaaaagac gatgcgtacg atgctgatcg tctctcctgc tatgatcgtc ttcatgtcga
    38041 tcagtgcgcc agccggtgtt acactttact gggtagtcgg tggtgtgttc agctgtctgc
    38101 aaacattcat cacaaacgtt atgatgaaac cacgtttgaa agcacaagtg gcagaagagt
    38161 tgaaacaaaa cccgccaaaa caagtcgtca ctccaagaaa agacgtgaca gaatcagcaa
    38221 agccagctga caacaaacaa gcaacaaaac aaatcaaaca accaaaaaac agtacaaaca
    38281 acaaaggccg caacgcagga aagcagcaga agcggaagta gatagtaaat aagagtgtaa
    38341 taaaaattca ttaacagttt tagcaattaa aaatatcgac caagattttt gaaaaagaaa
    38401 tctggtcgat atttttattt aagtgaatgt ttaatttgaa tcatcgattg atcgctatct
    38461 aattgcagca gcatcttttt atgaagaaac aactttttct tatctatcaa cgtagacagg
    38521 tgttgctgtt tcttttaata atgtacttaa ttccttaata ttttgttttc cttcttctaa
    38581 catccaacta ctcgcttttt tttcgccttc ggtaacaaaa ggtgccactc ccttcgccca
    38641 tgttgctcgc ccacataaga taccattgaa tgtcgaacct gattgttttg caaaacgtaa
    38701 tgtatcttga aatagcgtag cattgactcc cgcacttaaa aaaataaacg gttgttcagt
    38761 cactttcgat tgttcataga aatacgcgga tgcctctgct tgactataac aagtctcttg
    38821 cccatatcct tccacaaatc tcatattcac tggcacttct actttcaata catccacttg
    38881 ataacgttct tttgaaaatt ctcgcatggc ttcaataact ttatggggtt ttacttttgc
    38941 ataggcaaga gaagacggat cgccaatagt tgtatcataa gaaaggatct ctaaaaagaa
    39001 aggtatgcct tctgcttcgc attctgaacc aatccgttct ataactattt gtttttgcac
    39061 attaatctct gctggttcat ccacgtcata gtacaataaa aatttacaag catccgcacc
    39121 agcttctttt aaccttttca ccgaccaaat attcaataaa tcaggtaatc ttcccggcat
    39181 tttcgtatca tagccggttt tttcataagc tagcagcaag ccacattctt tctctcgaag
    39241 cgctgctgca ggcaggccgt attctggatc aagtaaaata gctgaggcat agggagtcag
    39301 gtattgagaa accatctgtt taaattggat gatgtcttct gatgtagctt ctttatattg
    39361 gttgatcatt ttcttcaaag caccacgctg gtcgattgct aacgcgctaa taattccagc
    39421 ttcatccgtc aatcgcttca aacgttcctg cttttctata ctcatcttct tcatatatct
    39481 tcacctattt ctttagttaa tattgtggct atttttgttt ttgattttgc ttttctcaac
    39541 tgttctgtaa cctcttttcg catcaatgat cgtgcaacga gagacaataa tttcaaatga
    39601 gtcgttcctg cttctgaatc tggaataaat aacgcaaaaa taaaatagta aatatctact
    39661 cgtgaaaacg ggtggttttt ggcagcccta gaaggcctca tcacttgctt atcctcttaa
    39721 gaggggcttt ttaagggacc accaacttgt accgaccccc aaaagttaga ccaaaaaatc
    39781 taacttttgg aggtcggtat tttttatggc taagtatact tttgaactta aattaaagat
    39841 cgttcatgat tatttggatg gcaaaggcgg aagtgattat ctggcaaaga a
//
LOCUS       DACDIE010000021        38338 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.021, whole genome shotgun sequence.
ACCESSION   DACDIE010000021 DACDIE010000000
VERSION     DACDIE010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38338
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.021"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            161..391
                     /locus_tag="IV250_001183"
     CDS             161..391
                     /locus_tag="IV250_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HAP8768479.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            488..1789
                     /gene="murA"
                     /locus_tag="IV250_001184"
     CDS             488..1789
                     /gene="murA"
                     /locus_tag="IV250_001184"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP8768480.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1802..1978
                     /locus_tag="IV250_001185"
     CDS             1802..1978
                     /locus_tag="IV250_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP8768481.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2113..2409
                     /gene="yidD"
                     /locus_tag="IV250_001186"
     CDS             2113..2409
                     /gene="yidD"
                     /locus_tag="IV250_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAP8768482.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2593..3060
                     /locus_tag="IV250_001187"
     CDS             2593..3060
                     /locus_tag="IV250_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8768483.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3145..4029
                     /locus_tag="IV250_001188"
     CDS             3145..4029
                     /locus_tag="IV250_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8768484.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4146..4379
                     /locus_tag="IV250_001189"
     CDS             4146..4379
                     /locus_tag="IV250_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HAP8768485.1"
                     /translation="MSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGP
                     KEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4467..4547
                     /locus_tag="IV250_001190"
     tRNA            4467..4547
                     /locus_tag="IV250_001190"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4501..4503,aa:Tyr,seq:gta)
     gene            4554..4625
                     /locus_tag="IV250_001191"
     tRNA            4554..4625
                     /locus_tag="IV250_001191"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4586..4588,aa:Gln,seq:ttg)
     gene            5140..5847
                     /locus_tag="IV250_001192"
     CDS             5140..5847
                     /locus_tag="IV250_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8768486.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5907..6551)
                     /locus_tag="IV250_001193"
     CDS             complement(5907..6551)
                     /locus_tag="IV250_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HAP8768487.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6678..6965
                     /locus_tag="IV250_001194"
     CDS             6678..6965
                     /locus_tag="IV250_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8768488.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            7073..7393
                     /locus_tag="IV250_001195"
     CDS             7073..7393
                     /locus_tag="IV250_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8768489.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7474..8787
                     /locus_tag="IV250_001196"
     CDS             7474..8787
                     /locus_tag="IV250_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP8768490.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATIQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            8784..9503
                     /locus_tag="IV250_001197"
     CDS             8784..9503
                     /locus_tag="IV250_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAP8768491.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            9631..10182
                     /gene="raiA"
                     /locus_tag="IV250_001198"
     CDS             9631..10182
                     /gene="raiA"
                     /locus_tag="IV250_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAP8768492.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            10404..12938
                     /gene="secA"
                     /locus_tag="IV250_001199"
     CDS             10404..12938
                     /gene="secA"
                     /locus_tag="IV250_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAP8768493.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            13064..14165
                     /gene="prfB"
                     /locus_tag="IV250_001200"
     CDS             join(13064..13126,13128..14165)
                     /gene="prfB"
                     /locus_tag="IV250_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAP8768494.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            14270..14956
                     /gene="ftsE"
                     /locus_tag="IV250_001201"
     CDS             14270..14956
                     /gene="ftsE"
                     /locus_tag="IV250_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAP8768495.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            14940..15833
                     /locus_tag="IV250_001202"
     CDS             14940..15833
                     /locus_tag="IV250_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8768496.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            15985..17433
                     /gene="cls"
                     /locus_tag="IV250_001203"
     CDS             15985..17433
                     /gene="cls"
                     /locus_tag="IV250_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP8768497.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            17770..18627
                     /locus_tag="IV250_001204"
     CDS             17770..18627
                     /locus_tag="IV250_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAP8768498.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            18742..19662
                     /gene="pstC"
                     /locus_tag="IV250_001205"
     CDS             18742..19662
                     /gene="pstC"
                     /locus_tag="IV250_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAP8768499.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            19662..20546
                     /gene="pstA"
                     /locus_tag="IV250_001206"
     CDS             19662..20546
                     /gene="pstA"
                     /locus_tag="IV250_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAP8768500.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            20561..21367
                     /gene="pstB"
                     /locus_tag="IV250_001207"
     CDS             20561..21367
                     /gene="pstB"
                     /locus_tag="IV250_001207"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP8768501.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            21383..22141
                     /locus_tag="IV250_001208"
     CDS             21383..22141
                     /locus_tag="IV250_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP8768502.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            22154..22831
                     /gene="phoU"
                     /locus_tag="IV250_001209"
     CDS             22154..22831
                     /gene="phoU"
                     /locus_tag="IV250_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAP8768503.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            23069..24100
                     /locus_tag="IV250_001210"
     CDS             23069..24100
                     /locus_tag="IV250_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP8768504.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            24097..24864
                     /locus_tag="IV250_001211"
     CDS             24097..24864
                     /locus_tag="IV250_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP8768505.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            24964..26532
                     /gene="liaX"
                     /locus_tag="IV250_001212"
     CDS             24964..26532
                     /gene="liaX"
                     /locus_tag="IV250_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HAP8768506.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            26832..27149
                     /locus_tag="IV250_001213"
     CDS             26832..27149
                     /locus_tag="IV250_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAP8768507.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDEW
                     SDF"
     gene            27149..27499
                     /locus_tag="IV250_001214"
     CDS             27149..27499
                     /locus_tag="IV250_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAP8768508.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            27666..28604
                     /locus_tag="IV250_001215"
     CDS             27666..28604
                     /locus_tag="IV250_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAP8768509.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            28619..29449
                     /locus_tag="IV250_001216"
     CDS             28619..29449
                     /locus_tag="IV250_001216"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAP8768510.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            29493..30518
                     /locus_tag="IV250_001217"
     CDS             29493..30518
                     /locus_tag="IV250_001217"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP8768511.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHHASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            30533..31471
                     /gene="galU"
                     /locus_tag="IV250_001218"
     CDS             30533..31471
                     /gene="galU"
                     /locus_tag="IV250_001218"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP8768512.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYIMSLGKTLEAEGSKINHNPKGITEIKEEKKK"
     gene            31596..32036
                     /locus_tag="IV250_001219"
     CDS             31596..32036
                     /locus_tag="IV250_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAP8768513.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTQDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIVTKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            32039..32641
                     /locus_tag="IV250_001220"
     CDS             32039..32641
                     /locus_tag="IV250_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP8768514.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSSELTGIAKEKAQGLSQQAGDLAGNVKQKVSESAEEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDLKKPADELGSIAKDTSEDIMIDVKDAADKAGETVNEG
                     MSEAKPVADDTKEMLKMAKEDAKEAKKEMKDTMTDSTTNH"
     gene            complement(32704..33807)
                     /locus_tag="IV250_001221"
     CDS             complement(32704..33807)
                     /locus_tag="IV250_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP8768515.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVDRFDAISDILTGTDSYTDLTSIIQRLQLIKTVEEKDKLIEAGKWADVAF
                     EIGFKTVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGSTKVSP
                     NELVLFDLGVIWNGYCSDATRTVAYQKPTEFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLE"
     gene            34017..35018
                     /gene="ccpA"
                     /locus_tag="IV250_001222"
     CDS             34017..35018
                     /gene="ccpA"
                     /locus_tag="IV250_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HAP8768516.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            35373..35768
                     /locus_tag="IV250_001223"
     CDS             35373..35768
                     /locus_tag="IV250_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8768517.1"
                     /translation="MPKKRRKTIVIILTILTIGIGGKFYMDKREMQRQKDLLAVEKQS
                     VKVLKNTFADIKEVKIEKSARNEMTGSYRIVILMTNKQDQSIYFSYSFWKERNEIGSY
                     GIVDEKKQKEGNTLNKVKVTYSNGNEESI"
     gene            35768..36019
                     /locus_tag="IV250_001224"
     CDS             35768..36019
                     /locus_tag="IV250_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8768518.1"
                     /translation="MITDKQYSELSDAVYWLDPKHRDYVPEMQENLSFKINGTMYKIL
                     KIKNSFDGMQAMAVAPIVHSKLEKNFKNKKIPANFRVLK"
     gene            36062..36457
                     /locus_tag="IV250_001225"
     CDS             36062..36457
                     /locus_tag="IV250_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8768519.1"
                     /translation="MSRKIRKLSLAILLIVILGIGGKVYMDKREAQRQKDLLAVEKQS
                     VKVLKNTFADIREVKVEEFKKNPVTGSYGALVTMTNNEGKSVYFDYSFWKERNELGGF
                     GIENRAVQKTGVTIKRVKVIFSNGQEELV"
     gene            36454..37710
                     /locus_tag="IV250_001226"
     CDS             36454..37710
                     /locus_tag="IV250_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HAP8768520.1"
                     /translation="MTINDKHYNDISEKVYWLDPKYPRYNEGYKKNSVKEFAGMEFQI
                     LQIKDSLDGMQAMSVAPIKNGKVDLSQVVIAFAGTNLSDWKDLETDARSIMNLYGNRP
                     GASFSEKATTNKILSVSGLKTAGQINSAIAFANEVKKEYGEAAISTTGHSLGGFLALY
                     VAAEKQWKNVGFNGPDSYDLLSPEAKKWVEENPGMLTNYRNHADFIGNFGGNGTGAEV
                     RISMETAFLNLLASHSLTSWKFDENGKLIIPENENNVKARKQQKENQATQLFSYQLQE
                     LNNIRKKLRLSGGQLTVNEQIYLDDTQARLVVEKLSAKMKSAMEEVIRINQRGIFELE
                     KKWQEGLRQARVVAPSLTEQEIIEALASVGATKENLVDSAKEVYLLRINKARRMGEAF
                     DTLAKEIQDKINEIVARDRELAQQFN"
     gene            37722..38045
                     /locus_tag="IV250_001227"
     CDS             37722..38045
                     /locus_tag="IV250_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8768521.1"
                     /translation="MKLEELERDYRSERRQLEAQEDELIHFKNQGLRLMEQEEQRLHH
                     VLKNLALDNEPLFQARRSYSNYQEHYQQALQVERRKLYQQQDELERSYRQKIRQITME
                     KSSYE"
     gene            38038..38304
                     /locus_tag="IV250_001228"
     CDS             38038..38304
                     /locus_tag="IV250_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8768522.1"
                     /translation="MNEFLMQLLKELEKNLHRSNVRSVGMGISQQIWAEKRLTDQNYQ
                     MTAEEAAQEFSNRHVHLKGSVKGQFAEKLTELFQRQEKKLKQFR"
BASE COUNT        12860 a         6513 c         8261 g        10704 t
ORIGIN      
        1 tttgtctcag tccctttttt tgcatatttt tagcatttat ttcctaaaaa atgtaagggg
       61 attcctatat ttttaagtat acttgttttt ccttttatac attttatggt attatcattt
      121 tattagttat tataaaacaa tcgttagaaa ggacgtcatt atgcaagtat acggaattga
      181 tgcggtgctt cgaatcgtga gtcatttggc gtttatctac ttagccttct ggtctttacg
      241 ttcgttacgc attgaaactt ttttcagacc aatgcataat acgcaaatta gaatggcaat
      301 cgtacttttt tcaatttttt tagggtacac tgcaagtaat tttttcttag aagttatcgc
      361 tttatgccga aatcttttcg ttagcctgta aattttaaaa aaaggtacta ttttaaaagc
      421 aaggatgtta taatagaaaa gattttgagt aagtgtatgc tcattggaat tttttggagg
      481 gaacaacatg gaagagatca tcgtaagagg tggcaatcaa ttaaatggaa cagtaagaat
      541 tgaaggagct aaaaatgctg ttttgcctat tttagctgct agtttgttag cagaagaagg
      601 tattacgaca ttagacaacg ttccgattct ttctgatgtc ttcactatga atcaagtgat
      661 tcgtcactta aacgtagatg tagattttga tgaacaaaag aatcaagtaa caatcgatgc
      721 ttcaagacaa ttagaaatcg aagcgcctta tgagtatgtt agtcaaatgc gtgcttcaat
      781 cgttgtgatg ggtccgctac ttgccagaaa tggacatgca aaagtagcta tgcctggtgg
      841 atgtgcgatt ggtaaacgac caatcgattt gcatttaaaa ggatttcaag ctttaggagc
      901 aaaaattatt caaaaaaacg gctatatcga agcaattgcg gatgaattga tcggtaacac
      961 gatttattta gatttcccta gtgtaggtgc tactcaaaat atcatgatgg ctgccgtaaa
     1021 agcaaaagga acaaccatca ttgaaaacgt agctcgcgaa ccagaaatcg ttgatttagc
     1081 aaacattttg aataaaatgg gcgctcaagt ctatggagct ggtactgaaa caatgcgtat
     1141 tgaaggcgtg gatcatttac atgcagtcaa ccactcaatc gtacaagatc gtatcgaagc
     1201 aggtaccttt atggttgctg ctgcaatgac acaaggcaat gtgctgattg cagatgctat
     1261 ctctgaacat aaccgcccat tgatttctaa attgatcgaa atgggtgcag aaatcattga
     1321 agaagaaggc ggagtccgtg tcatcggacc aaaacatatc ttacctacag atgtaaaaac
     1381 gatgcctcat cctggtttcc ctacagatat gcaagctcaa atgacagcta tccaattagt
     1441 tgcagaaggt acaagtgtag tgactgaaac agtgtttgaa aatcgtttcc aacatttaga
     1501 agagatgcgc cgtatgaacg ctcatgtcaa aatcgacgga aatgtagcaa tcatggatgg
     1561 aaaccatgaa ttgcaaggtg cagaagtata tgcaacagac ttgcgggcag cagcagcatt
     1621 agttttagct ggcttgaaag caaatggaat cacacgtgtg cgtaacttga attatctaga
     1681 ccgtggatat tataatttcc atatcaaact acagcaatta ggtgcagacg ttgaacgtgt
     1741 agacatggat caaacttctg ctgaaaaaac agcacaaacg attgcttaag gaagtggctg
     1801 aatgagtaac caacgatata tcttattaac tttgataaag attttagtgg tgatcttatt
     1861 gcttatcctt ctttttgtgg ccggaacgat gatcggatat ggtgtcatcg gtggcggcaa
     1921 tccatttaaa gtattccagc caagtttgtg gattcatatt cgagactttt ttcattgata
     1981 aataagaatc acagtggttc tagtcgatat gaagtgagcc atgaagcaga acatgacaaa
     2041 agaaaacaga agggaacgtt gacgttagtc gcgatccctt tttttagtaa aagaaaagaa
     2101 ggaatctaat tgatgaagaa gatattgatt tatttagtta gaggatatca gcgctttatt
     2161 tcgcctgcat ttccacctag ctgccgttat tacccaacct gttccaatta tatgataaaa
     2221 gcaattcaaa cccatggtgc actaaaaggg acggtaatgg ggactgcacg gatcttacgc
     2281 tgtcatcctt ttgtcaaagg cggtatcgat tatgtaccat tgacattcca attaacgcga
     2341 aataagaaag ataaaaaaga accagattat aaaaaggttg tgaaaaatac gagtagcact
     2401 gagcgataaa gaaacaattt ggaaaatagc ttcttatatt ttgcaaaatt gtgagttatt
     2461 gccgaagtgt tggattttga acaccgtcaa aaaaaggttg tgaaaaatac gggtagcact
     2521 gagcgataaa gaaaaagagg aaaattattg tcaaagtaac aactccgcaa ctacttatat
     2581 gggggacaaa aaatgatcga agaactaaac aagaaagatt taccatggtc cttgttatta
     2641 gatgcagatc ctgataaaga aaaagttcaa gtatatgttt ccagagggag tggctggata
     2701 tggaaagaaa aagaaaaaac aataggtgta ctgatctatg tggcaaggga aaaagaatac
     2761 gaaatcgtca atgtagcagt agccccaacg tatcaaggaa aggggattgg cggaaaatta
     2821 cttgagacag cttttcagaa gctttcacaa ctcgcttcca gtcaaactca gatcatcata
     2881 cgaaccggca gtactagcag tgcggcgtta catctctatc aaaaaatggg ctatgtggag
     2941 attggtcgag tcaaagacta ttttatccat cattactctg agcccatttt tgaaaatgga
     3001 gatcaattaa gggatcaagt aattttagca attcacttac ctaaaaatag ctttaactaa
     3061 taaactttca attcgagaaa atgctttcaa atgctaaata gatacattac aatagcttta
     3121 gcaaacaaaa aggagaatat ttttatggga atattagttt ttgattttgg tggatcggct
     3181 gtcaaatatg gttgttggga tggcaaagag attaaaggta agggacaatt tgcaacgcct
     3241 gaatcttggg aagaaatgaa agcgcaatta tttcaagttt ataaaaaaat actcgtttct
     3301 tttgaagtcg aaggaattgg aatcagctct cctggagtag tgaataatga gaaacaggtg
     3361 attgagggaa tcagtgcgat tccttatatt catggattta atattttccg agatttggaa
     3421 actctgtttc aactcccagt aacgattgaa aatgacgcca actgtgccgg aatggctgaa
     3481 ttttatgaag gtgctgcccg tgattatcaa gatgttgctt ttgttgtggc aggaactggg
     3541 attggcgggg cgttgttcac gaaaggacaa atcaataaag gggcacatct ttatggtgga
     3601 gaatttgggt tgatgttctt aaatggcgat aagacattca gtaaacttgg tacagcggta
     3661 cagatggctt ggcgttattg tgaacgcaaa ggtctcgata aaaattctta taccggaaaa
     3721 gatgtctttg aattagcaga aactggagat gcaatagcaa aagaagaagt tgagagtttt
     3781 tatacgtatc ttacaaaggg attattcagt attcaatttt cgtttgatcc agaagtgatc
     3841 gtattaggcg gcggtgtttc tgcaaaagaa gggctcattg atgagatcaa aagccgaatg
     3901 aagaagctga cagaacaatt cgatctgcat gatttcgaac cgcagatttt attatgtgaa
     3961 tacagaaacg atgctaatct tgtaggtgca gcagcaaact atctcgctgt acgtcaaggg
     4021 gagaattaag agctttagag aaggtgcagc ttgcataaaa gaagtgaatg aaatgaatag
     4081 gagattttta cctaaggaat gttttatttg ctgcagtatg ataaaatatc tgagaggtga
     4141 aaatgatgtc aaaaaaaaat aaagatgttg aagtgcgtat agaagaagtg aaaaaagata
     4201 tcaaaggtaa gaattatgaa gtcactcagt tatttatagg gaaaaaaatg attggtgaga
     4261 tcttagccta tgggccaaaa gaatatgaaa ttttctttgg tgaagaagat tttggaaaag
     4321 aaaaaagtct tgaaaatgcg atagaaacag tgattcgaca ctggaatctg cacgaataaa
     4381 aaaatatcaa aaaaactttc aataaagggt tgcaaaaatc tggaaaatta ggtattataa
     4441 ctaagtcagg ttgatacaaa cctttcggag gagtagcgaa gtggctaaac gcgacggact
     4501 gtaaatccgt tccttagggt tcagtggttc gaatccactc tcctccattt cattggggta
     4561 tagccaagcg gtaaggcaag ggactttgac tccctcatgc gttggttcga atccagctac
     4621 cccagtatac cacaatcgta agctctcgtt agttcgagag ctcttttttt tgctttgcta
     4681 ccttgttcct tgaagattct gctgatttaa gatagagtta agttaactac aagaattgga
     4741 gggatccaca tgaaatggaa agaaaaagta gacgaagcag ctgaaaagtt atatgatttg
     4801 gtcaaatcag aaaaatacaa tattgaagtg aatattccga aaaaaggcgg aaaagcggtc
     4861 caggtaaaat caaaacgccc aacgaatcat acaaaaaaat ggatggctaa aaaccgctaa
     4921 taataacaaa agatccataa gtcggcattt tcatcgattt tagggtcttt tgttattttc
     4981 taggttgaac atatctaaaa aattgtttct gacaatagga gtaggaagaa cgatgtgtgt
     5041 ctaaatcctc tgttgatgga tagaaacctt tatctttttc agttgtgaat caggtatata
     5101 ccagttataa tagaagaaac gaaaggacgg gtgtacgtta tgtcgaatca attacctgta
     5161 tatatacaga ttcatgacca attaaaaaat gaaatcgaac aaggtatatg gaaagtcgga
     5221 gaccgtcttc cttcagaaag agaattagca gtgaaattca atgtcagccg tatgacgttg
     5281 agacaagcaa ttcaaaactt ggcagatgaa ggaatcttag aaagaaagat cggttcgggt
     5341 acatatgttg cgcgagaaaa agtacaagaa aaaatgtctg gtgcaacaag ttttacagaa
     5401 attatggaat cgcaaaatcg agtaccgtct agtcggacaa tctcttattt cttgacttcg
     5461 ccaagctcaa gtgaaatgga aaaactaaac ttgagtaaag atgacacgat cttgcgaatg
     5521 gaacgtattc gtttcgcaga tgatgtccct atttgttttg aagttgccag tgtgccacaa
     5581 aagctgatcg atggctacag caaagcagag attactgaat cgttgtatcg cacgctagaa
     5641 gaaaaaggcg ggaaaaagat cggtccagcg aatcaaacga ttactgctgt gttggcttct
     5701 gagaaaattg ctgactattt agaggtaaaa cgaggagatg cagttcttcg tttgcgccag
     5761 atttcttatt ttgaagacgg tacgccgttt gagtatgtgc gtacgcaata cgtaggaaat
     5821 cgttttgagt tttatttgga aaaataaaaa acaggtgatc cctgattcct tacttagaaa
     5881 taaaagagaa tcagaatcac ctgtttttat atatgatcag ttaccagttg ctcgtgataa
     5941 gaaacaggac cctcttcaaa ttggacttgt ccgttcagca gctcaatgat ggattcttta
     6001 aagtggtcag cacttttttc atctacagca cagatgacgc taacttgatc agtatattgg
     6061 atttctttta taaaaaaatg ttggagttca agataattct gtaattttcc tagttgcgga
     6121 tacgtgatca tcacagagat ctcctgttgc aaggtacctt ctacgattcc aatgtcagac
     6181 aatgcttgag agacagcatg agtgtatgct cgtattaatc cgcctgtgcc gagtttcgtt
     6241 cctccaaaat atcgagtgac tactgcagct acattgatca gttgattttt tttgaggact
     6301 tccaacatcg gtacgccagc cgttccactt ggttctccat catcgctgct tcgctggatt
     6361 tcattttttt ctccaatgat gaaggcgctg cagttatggt tcgctttcca atgttctttt
     6421 tttatttctt gaatgaaact ttttgcttgg tcctctgatt ctattcgtct aagagaacag
     6481 ataaagcgtg attttttgat ctcaatctca ctttgtccat tttcttttat tgtacgatag
     6541 cgctcgatca tcttgtcacc tctttttttc attttcttta ttcagtataa ataaagttct
     6601 aggttgtttc aacactgtaa cggttgcgca atcgctgtag tatgcgataa taaagacaaa
     6661 ggtaggtgga tctggggatg caggaacgaa tgaaaaagta cgatgcaatt acgcattatt
     6721 tgaagaacaa tggcggctct caagtaacgt tgacctttac ccaatttgac gaactgctgt
     6781 ttcccagtaa cggacttccc aaaacagcaa gggaaagtac agattggtgg gcaaatgatt
     6841 ataaacaccc tgaaaaaggt gcctatggct ggataaatgc aggctatgaa gtagttgtca
     6901 tcaatttgga caaagaatat gtcgtgttca ataaattagt gaaatctagt tggctatttg
     6961 attaaataaa ccgcgtttag aaaacgttga caaatttctt tctttttata taatagagta
     7021 acagacgcga agaaaacgtt tgcgtagttt gttttaaaaa ggagagaaaa gtatggctaa
     7081 aaaaacaatc atgcttgtat gttcggctgg tatgagcaca agtttactag taacaaaaat
     7141 gcaaaaagct gctgaagcaa aaggaatcga ctcggatatt ttcgctgtgt ccgcttctga
     7201 cgcagataac aatcttgcaa ataaagatgt agatgtactg ttattaggac ctcaagttcg
     7261 ctttatgaaa gctgattttg aaaaacgtct ggaaccaaaa ggcattcctt tagacgtcat
     7321 caatatggct gattatggta tgatgaatgg cgaaaaagtg ttagatcaag caatcacgct
     7381 cataggtgaa taaatagaac agtataacta tcgaacaagc gatccaaaaa atagatcgct
     7441 tgttttttcg tatatttttt tagggcgaat aatatgaatc atttttgggg tagaaggctt
     7501 ctgacgagag atattaaagc aatagactgt gaggaggtaa atttaccaaa ttatgagaag
     7561 atcgcagcag tggaacgttt gggcaactgg attctttgtc atcgctgtgg aatgaggacg
     7621 tccgtcattg atggacagct tgcagattac ggttattttt gtacacattg tctatattta
     7681 gggcgatgcg acactcaaca agatttatat ttgtttgatc agcctgaagc aaaacctcga
     7741 gaagtcgtct ttttttggac aggacagctt actgaaaaac aaacggaaat cgcaaagaga
     7801 atactttgcc attcagaaaa aagacatcat cttatttggg ctgtaacagg tgcagggaaa
     7861 acagagatgc tttatcctat tttagtggaa actttaaaag ctggaggaag agtagcgatt
     7921 tgtacaccgc gagtcgatgt ttgtaatgaa ttgtttttac gctatcgtta tgtttttcca
     7981 gaagaaaaaa tcacgttgct ccatgggaat acagcaacgg cttatacctt tacttctttt
     8041 gttatctgta cgattcatca gctataccgt ttttatcggt cctttgattt aatagtgatc
     8101 gatgagatcg atgcgtttcc ttataacggg gatgcaggat tagctcatgc tgtgcaaaca
     8161 gcgattaaac cagacggacg attcatctac ttaagtgcca cacctgataa aaagttacta
     8221 aaggaaatca agcagacgtt tgacatccac aaacttgctt tgcgctttca tcggcgttta
     8281 cttcctgaac ccgtcttggt tttcatgaat aattggcgac aaaaatgcaa gaacaagaag
     8341 aagataaaat ctttggtgcg gatcattcat caattaatta aagaaaacca tatcctttta
     8401 ttttgtccaa gtatcacgat gatgcatcgt ttgaaagcca caattcaaga ggctcttccg
     8461 agatatgaga tcacagatgt ttcttcaaga gacaatcagc gggcggaaaa agtccagaaa
     8521 atgcgagaag caaaatatga catactgtta accacgatga tccttgaacg tggagtcaca
     8581 tttgaaagaa tatccgttat cgttttagga gcggatcatc cggtttttac taaatcttct
     8641 ttggtacaaa tcgcaggaag agccgatcgt aaagggcctt atacgaacag tcaagtatac
     8701 tttttctatg aagaaaaaac tgcagctatt cgaaaagctt gtcaggaaat caaagaaatg
     8761 aatgaagaag ggaagaagat gctgtgaggt gcagttgctg cagtaaagat atcgtccaaa
     8821 atttaacatt cagcgagatt attggtgttt cgaaatacag attacctcag acttgttcag
     8881 tttgtcgcgc acaatttcat ttattggaaa caagaggctg ttctggctgt caaaaaccaa
     8941 tccatttcaa tctagagagt acgaaaaaga aagtaaagtc tattcccgtt tactgcagtg
     9001 actgtcaaga atggaagaag aaatatccga cctatgattt tcagcaccgt gccttttttg
     9061 tgtacgatga aaatatgaag gcctggctca atcaatacaa gtttatggga gatatccgac
     9121 tagcaggcac ctttgcatat aaatggacgc agataaaaaa agaatacccg acatacatct
     9181 attgtccaat acctttatct gaaaaaaggc gaacagaaag aggattcaat caagtaagcg
     9241 agatgttgaa agcagcaaga attccttttc agatgctact caaaagagag aagcatctgc
     9301 tggctcaagc aaaaaacacg cgaaaggaaa gattggagat gcctcaacct tttgaacttg
     9361 cagtcacctt ttctaagata caaaataaaa aaatattgat tattgatgat gtctatacaa
     9421 caggacagac tcttttccac gcagcagatt gtttgcaggt cgcacagccg aaaatcatcc
     9481 gaaccatttc tttagcaaga tgaattagat gtggaaattt gatagcgatt taatttgcaa
     9541 ataacctaga gttcgatata atagaattaa gaagagggaa agagtggtcc ttctccttct
     9601 tgattgtggt agacgaaagg ggtaattttt atgtttagat ataatgtacg cggcgaaaat
     9661 atcgaagtaa ctgaagcgat tcgcgactac gttgagaaaa aagtaggcaa actagaacgc
     9721 tatttcagtg acgcacccga tgcaacggcg catgtgaact taaaagttta tacagaaaaa
     9781 acagcaaaag tggaagtaac gatcccatta ccttatcttg tattacgtgc cgaagaaact
     9841 tctccagatt tatacgcaag tatcgatttg gttgttgaca aattggaacg ccaaatccgc
     9901 aaattcaaaa caaaaattaa tcgtaaatcc cgtgaaacgg gcatgaatac tgcagatgct
     9961 actgctttat tcaatgaaga ggacgaaaac gaaaatgatt ctgaattaga tatcgtacgt
    10021 acgaaacgcc tttcattgaa accaatggat agtgaagaag cagtcttaca aatgaacatg
    10081 ctgggacata acttctttat ttttgaagat gcagaaacaa atggtacaag tatcgtttac
    10141 cgccgtaaag atggaaaata cggactgatt gaaacagact gatcagcctt acttaaataa
    10201 caacgaaaac ttgcagcagc tactgacagc tgccgcaagt ttttttgagt ttcctcctct
    10261 tttagatgaa atatgaataa attatgaagt tcttacttta atacgcttaa gatatttcaa
    10321 ttatctataa ctaatgataa aatgaaaagt aaagtctaca tagtctggat aatacacaga
    10381 aggaaaagga gattacacaa cgcatggcca atttgattcg ttcacttatc gaaaacgata
    10441 agaaagaact aaaacgttta gatagcattg caaaaaaagt tgaagcatac gctgaccaaa
    10501 tggcagcatt aactgatgaa caattacagg caaaaactgc tgaattcaag ggcagatacc
    10561 aaaaaggaga aacattagat caattacttc cggaagcttt tgcagtcgtc cgagaagcag
    10621 caaaacgtgt attggggtta tatccacacc acgtacaatt gatgggtgga atcgtgcttc
    10681 acgatggaaa tatccctgaa atgcgtactg gtgaaggtaa aaccttgacc gcaacgatgc
    10741 cagtatatct aaatgctctg actggcgaag gggtacacgt agttaccgtc aatgaatatt
    10801 tagcaacccg tgactcgacg gaaatgggcg aactttataa tttcttagga ttaacagtgg
    10861 ggttgaatat caactcaaaa tcttcggatg aaaaacgcga ggcttataat tgtgatatca
    10921 cttatagtac gaataatgaa ttaggatttg actacttgag agacaatatg gtcgtttacc
    10981 gccatcaaat ggtacaacgt ccgctgaatt atgccattgt cgatgaagtt gactcaatct
    11041 tgattgatga agcgagaacg ccgctgatca tttcagggca agcagaaaaa tccacagctt
    11101 tatatacaag agcagacaac ttcgtgaaac gtttgaaaga agaagaagat tataaaatcg
    11161 atgtacaatc aaaaacaatc agtttaaccg aagcagggat cgaaaaagca gaagaaacat
    11221 tcggtttgga taacttatac gacattgaaa atactgcatt gacccatcac ttggatcaag
    11281 cattgagagc caactatatc atgcttttag atatcgatta tgttgtccaa gacggaaaag
    11341 tactgattgt tgaccagttc actggtcgga tcatggatgg acgccgttat tcagatggac
    11401 ttcaccaagc aatcgaagct aaagaaggcg tagagatcga agacgaaacg aaaaccatgg
    11461 cgacgatcac tttccagaac tatttccgta tgtacaagaa acttgcggga atgactggta
    11521 cagctaaaac agaagaagaa gaattcagag aaatttataa catccaagta taccagattc
    11581 cgacaaatcg tccggttatc cgtgatgatc gtccagattt gctttaccca acattacaaa
    11641 gcaagttcca cgctgtcgtg caagatatca aagatcgcta ccgcaaaggt caacctgtgt
    11701 tagtcggaac tgttgcggtt gaaacatctg agttattatc agatatgctg aatcaagaac
    11761 gtattcctca tgaagtgctg aatgcgaaaa atcactttaa agaagcagag atcatcatga
    11821 atgctggtca aaaaggtgct gtgacgattg ctaccaacat ggctggtcgg ggaacagata
    11881 tcaagttagg acttggggta agagaacttg gtggtctagc agttatcggg acagaacgac
    11941 atgaatctcg tcgtatcgat aaccagcttc gaggacgtgc aggacgtcaa ggagaccctg
    12001 gtatgtcaca gttctacttg tcattggaag atgatttgat gaaacgattc ggttcagaac
    12061 gaatcaaatc tttcttagat cggatgaaga tcggtgagga agatgctgtt atccaaagca
    12121 agatgctgac gaaacaagta gaatctgcac aaaaacgtgt agaaggaaat aactatgaca
    12181 cacgtaaaaa cgtcttacaa tacgatgatg tgatgcgtga acaacgtgaa gtaatctata
    12241 aacagcgaca agaagtaatc atggaagaaa aaagcttatc caagccacta atgaacatgg
    12301 tcaaacggac aatcagtcgt gtagtagatg cgcatacaca attagagaaa gaaaattgga
    12361 atttggaaag tatcatcgat tttgccggca gtactcttgt ccatgaagat agtattactc
    12421 ttgcagatat cgaaggaaaa acagctcagg agatcaaaga gtatctaaac gaacgagcac
    12481 aagaagtatt taataccaaa gcggctcaac taaatggtcc agagcaactg ttggaatttg
    12541 aaaaagtcgt gatcttgcgt gttgtggact ctaaatggac agaccatatt gatgcaatgg
    12601 atcaattacg tcagtcaatc ggcttacgtg cttatggaca gaacaatcca ttagtcgaat
    12661 atcaaacaga aggctataag atgtttgaag atatggtagg cgccatcgag tatgaagtca
    12721 ctcgaatctt tatgaaagct gagatccgtc aaaacgtaca gcgtgaacag gtttctcaag
    12781 gaacagcttc tcactcagaa gacggagatg ccaaagaaaa tagtaatact agcgcgaaaa
    12841 aacagccaat ccatgtgaac aaggtcggaa gaaatgatcc ttgtccttgt ggaagcggga
    12901 aaaaatataa gaactgtcat ggaaaaaatg caaactaatt tctgacagca ggattggtga
    12961 gacaaattct ttttttaaga attcgtctca ccttctttct gttatttgct gattctgtta
    13021 gactagatat aatgaaaaaa gtatagtgaa agaaggaaaa aacatggaaa tatccgaaat
    13081 tcgaaatctc ctgacagaaa tgcaggaaaa ggtcacaagc ttcaggaggt ctctttgact
    13141 tagaccagct ggaagaagac attgcggaag cagaagcaaa aatggctgaa cctggttttt
    13201 gggatgatag cgaagctgct caaaaagtga tcagtgaaaa caatgcaaat aaagaaactt
    13261 atgatcaatt ccattctctt gctgaagaac tggaagaaat cgaattgatg gtggagatgt
    13321 tgcaggaaga acctgatgca gccatgcaag aagaagcaga aacacgtatc aatgcgctag
    13381 atgaaaaaat gaagacatat gaattatcta tgttgttaga tggtccttat gatcgaaaca
    13441 acgccatcgt tgaattgcat ccaggggctg gcggaacgga gtcccaagat tggggcagta
    13501 tgcttttacg tatgtataca cgctgggcag agcagcatgg gtatcaagta gagacactag
    13561 attatcaagc aggtgatgaa gcaggaatca agagtgtgac cctactgatc aaaggacaca
    13621 acgcatttgg ttatctgaaa tcagaaaaag gtgttcatcg ccttgtacgt atctcgcctt
    13681 ttgactcagc gaaacgacgt catacttctt tttgttcggt agatgtgatg ccagagcttg
    13741 atgaaacgat cgatatagaa atcaatccag atgatttgaa aatcgatacg taccgagcaa
    13801 gtggtgcagg tggtcagcac atcaataaaa cagaatctgc tgtacggatc actcacttgc
    13861 ctacaggaac agtagtagct agtcaggcac aacgttcaca attgaaaaac cgtgagcaag
    13921 cgatggggat gttaaaagct aagctttatc aattagaagt ggaaaagaaa gaacaagaag
    13981 cagctgcact tcgaggagaa caaaaagaaa tcggctgggg ttcacagatc cgttcttatg
    14041 ttttccatcc atattctatg gtaaaagacc atcggacaaa cgtagagaca ggaaatgtcc
    14101 aagcagttat ggacggtgac cttgatttat ttattgacgg ctatttaaaa atgcatctac
    14161 aataagaacc cattgttaaa ttgaaataaa attgtaagat tttgcaagca tgttgaaaca
    14221 ttgccatgct ataatagcta aagactgaaa aagatatgga gagaacgcca tgattgaaat
    14281 gaaggatgta atgaagaagt actcaaatgg tacgaccgct atccgcaatc tctcaattgt
    14341 catcgaacaa ggagagtttg tctatgtggt tggtccgtct ggagccggta aatctacatt
    14401 catcaaattg atgtatcgag aagaaaaagc ttcaaaagga actttgaacg tcgcaggtca
    14461 tgatctaata gcaatcaaaa accgcgaagt tccttatcta cgcagagaaa tcggcgtggt
    14521 gttccaagac tataaattat taccaagaaa aacagtatat gaaaatgttg cgtacgctat
    14581 gcaagttatc ggcagaaaac ctcgggatat caaaaaacga gtaatggaag tgctggacct
    14641 tgttggtttg aaacacaaag ttcgagtctt tcctagcgaa ttatccggtg gggaacagca
    14701 acgtgtctct attgctcggg caatagtgaa tacgcctaaa gtacttatcg cggatgaacc
    14761 gacaggaaat ctggatccag aaaactcatg ggaaatcatg aaattattag atcggatcaa
    14821 tgcccaagga actactgtcg tgatggctac acataacagt acgatcgtaa atacgattcg
    14881 gcaccgagta attgctatcg aaaacggccg cattattcga gatcaagcgg aaggggaata
    14941 tggatacgat gattagaacc tttttccgcc atttattaga aagtatcaag agtttgaaac
    15001 gtaacggctg gatgacagtt gcttcagtca gtgcggtaac gatcacattg actttagtcg
    15061 gtgtattcat ggcagttatc atgaacgcaa ctaaattagc tcaagatata gaaggaaacg
    15121 ttgatgtctc tgtattcgta gatatcggta caaaagacga agagatgaaa aagctagaaa
    15181 aagaattgaa tgacttagat catgttaaaa aagttgaatt ttcaagcaaa gaacaggaat
    15241 tgaaaaaaat ccaaaacgaa atgggtgatt cctggaaatt gtttgaaggt gacaataacc
    15301 cgttatatga tgtgtatgta gtaagtgcta ctgatccttc ttatacaaaa caagtagcag
    15361 aagaagcagc agatttgaag aatgtttctc gttctgatta cggtggagct tcctcagatc
    15421 ggattttcca aatcgctggt gcagttcgga catggggatt agttgcagcg gcattgctgc
    15481 tgttcgtagc gatgtttctc atttcgaaca cgattcggat cacgattttg tctagacagc
    15541 gtgaaattca aatcatgcgt ttagtcggtg caaaaaatgg atatattcgc tggccgtttt
    15601 tccttgaagg tggctggatc ggtctgatcg gtgcgattct tccgattttg atcatcacat
    15661 ttggttatgc atggtttttc caattgatca atccatcatt attacgttcc cattattcat
    15721 tgattcatcc acaaaatatc gtttggaaaa tcaatctttt aatggtcggc attggtgtga
    15781 tcattggttc acttggctcg atcatttcta tgagacgttt cttgaaattc taataccaca
    15841 atataaataa aatgagaaag ttggacagtt tcgttccagc tttctttttt tattggctag
    15901 gagaatagaa ggaagcggct tttttccctt tactttaaaa aacaacttgg gtattcttaa
    15961 gttggtagaa gaagaggtga agccgtgaaa attatcgttg acaatttttt tacgatcctt
    16021 ttgattatga atattttgct ttcctttatc atcgtttttc gagaaagaaa ggaaactgct
    16081 caaacatggg catgggtgct cgttttgatg ttcattccag ttgtaggatt tattttatat
    16141 atttttttag gaagagggat ttccaaagag aaaatctttg atttgaagat ccaagggaaa
    16201 atcggtaaga acctggaaat tgaagaagac aaacaggcat tgatgcgagg gctttatcct
    16261 catccgccta ctggacaagt agatgtgaaa caattgatct atatgctcac ggtatttgaa
    16321 agtacgctct atacgactaa aaatgaaatc actttattta cagatggtcg tgaaaaattc
    16381 gatgcgctga ttgaagatat caagcaggca gaagaccata tccacttcca gtattatatt
    16441 tatcgcagtg atgcattagg tgaagaagtt cgagatgcat tgacagaggc ggcaagacga
    16501 ggggtaaaag tcagagtcct tcttgatgca tggggttcca cacaagtttc ttccagcttt
    16561 tttcagaatt tgaaaaaagc aggcggagaa gtcgcatttt tctttcctct atttgttccg
    16621 tatattaatc ctcggatcaa ttatcgcaat catcgtaaaa tcgtagttat tgatgggaaa
    16681 atcggatata caggtgggtt caatgttgga aatgaatatc taggattggt aaagaaattt
    16741 ggttattggc gagacaatca tttgcgtatt tacggagaag cggtgtatac cttgcaaaac
    16801 cgttttttga tggactggaa ttctcagcat acaaaagaag taggctattc tcccaaattt
    16861 tttccgtcca ttcactctac tggtgatata gctgttcaaa tcgtgacaag cggaccagat
    16921 accgaacatg aacaaatcaa aatgacttat ttgaaaatga tttcgttagc caaacgggag
    16981 atattgatcc aaacgcctta ttacataccg gatggatcga ttcatgaagc actgaagttg
    17041 gctcttcttt caggcgtaaa agttcatatc caaataccta acaaaccaga tcatcttctc
    17101 gtttactggg caacgtattc tttcgctgca gagctaatcg agtatggagc aaggatcgaa
    17161 acatatgaga atggatttat tcatgcaaag acgatgatta tcgatggtgg cattgtttca
    17221 gttggctcag caaatatcga tgttcgttca tttcgcttgg actttgaggt caatacactg
    17281 gtttatgatg aacgaatggc ttcacgtgta agaaaagctt tttttgaaga ctcgaaaatt
    17341 tctacgcatc tcacaaaaga aatgtatgaa aaccgaggaa taatcatcaa gatgaaagaa
    17401 ggattagcaa gattgatttc gccgctcctt taaaacataa aaaggacatg agaaccatca
    17461 acagatggag atctcgtgtc cttttctaac ggggccattt ctgagagtac gtcagaaatg
    17521 gagaaagcgt ctcgcatgag cgaaattaac tttggggtat ctcttgtttt ttggggaaag
    17581 caagaaataa gctaatatat gctattaaaa gagacacatt tcaaagccag tatagcatag
    17641 aaacttttaa tgagatagtt ttttttacaa ttatgagaat ttattttgtt atttaatgat
    17701 tttgattcgt tttcatcaag taatgaaaat gataaaataa acatacttta ggattaaagg
    17761 agatttctaa tgaaaaaact taaattattc ttaccaataa taggattagc tattctttta
    17821 tcaggctgtt caaagtggat tgatcgagga gaatccatta cggctgttgg ctcttctgct
    17881 ttacagcctt tagtagaaac agttgctgaa acttaccaga cagaaaatcc tggaaaattc
    17941 atcaatgtac aagggggcgg aagcggaact ggtttgtctc aagtacagtc aggagcagtc
    18001 gacatcggaa actctgattt gtttgcggaa gaaaaatcgg ggatcgatgc ctcagcatta
    18061 gtcgatcatc gagtagcagt tgttgggatc actcctattg tgaacaagga tattggcgta
    18121 aaagacatca gtatggaaaa cctgaaaaaa ctatttacag gcgagatcaa aaactggagt
    18181 gaagtcggtg gaaaagatca gccagtagtt atattgaacc gcgcttcagg aagcggaacc
    18241 cgtagcacgt ttgaaaaatg ggtattggac ggagaaacag ctgttcaggc acaagaacaa
    18301 gattcaagcg gaatggtgcg ccagatcgtt gccgacacac ccggtgcaat cagctatgtt
    18361 gctttttctt atgtttccga tgatgtagat acgttgaatg ttaacggtgt agaaccgaca
    18421 gatgaaaatg tgacaacgaa caaatggacg atttgggcat atgagcatat gtacacaaga
    18481 gatcatccaa aaaaactgac aaaagaattt ttagactata tcttatctga tgaagtacaa
    18541 gataacatcg ttggagaact aggttatatc cctgtttctc atatgaaagt cgaacgtgat
    18601 tggcaaggaa atatcgttaa agagtaatct ttacacaaaa tttacaaact tagctaaact
    18661 tttacatgtt attaaaacca aattgatgtt caatttgtac actgagtgag tatgttaaac
    18721 ctgattaagg gggagtcatc tgtggaagat gtgaaaaaag tattattaac aaaatcaaaa
    18781 cgttcaaaaa tggaacagcg tggaaaattc atcagttttt tatgtatcgc gctgatcgtg
    18841 ttggttgtat tatctatttt ttattttgta gctagtaaag gattggccac cttttttact
    18901 gataagatca atgtatttga ttttctgttt ggtacagatt ggaatccaag ccaaatcgga
    18961 gcaaatggga aaccaatggt aggggcattg cctatgatca caggttcgtt tattgtgacc
    19021 tttttatctg ctatcgttgc cacgccattt gccattggtg cagcagtatt catgacggag
    19081 atctctccta aaaacggaac gaagatctta cagccggtca tcgagctgct tgtaggtatt
    19141 ccttctgtcg tttatggttt tatcggttta tcagttatcg tgccttttgt cagaagtatt
    19201 tttggcggga ctgggtttgg tatcttagct ggtacatttg tgttgttcgt tatgatcttg
    19261 ccgactgtca caacgatgac tgttgatgca ttaaaggcag tgccacggca ctatcgggaa
    19321 gcttcattag cgcttggtgc cacaagatgg caaacaatct acaaagtcgt gctacgagca
    19381 gccgtgcccg ggattttaac agcagtcgtt tttggtatgg ctcgtgcgtt tggtgaagcc
    19441 ttagctatcc aaatggtcat cggtaatgca gcattgatgc caacaggtct tactactcct
    19501 gcttctacat tgacgtccat cttaacaatg ggaatcggaa atacaatcat gggaacagtt
    19561 gaaaataatg tgttatggtc attagcgctg atcttgctat tgatgtcatt attctttaat
    19621 atcgtgatcc gtatgattgg gaagaaagga gagctgaaat aatgaacgca aaacgctcag
    19681 ataaaattgc tacaggtgtt ttatatatag tttcggggat catcgtattg attttagcag
    19741 ctttactact ttatatctta gttcggggaa taccgcacat ttcatggtcc ttcttgaccg
    19801 aaccttccaa agcctaccaa gaaggcggtg gaatcgggat ccagctattt aactcgattt
    19861 atttgctttt gatcacgatg atcatcagtt ttcctatttc tttaggtgcg ggtatctatc
    19921 tttcagaata tgctggaaaa aactggctaa cagatgtgat tcgcacgtcg attgaaatct
    19981 tgagctcgtt gccttctgtc gttgtcggtt tattcggttt cttggttttc gttattcaat
    20041 tccagtatgg tttctcgatt atttctggtg cattggcttt gactgtattc aacttgcctc
    20101 ttttgactag aacagttgag gagtctttac aagcggtcca ttacacacaa cgagaagcag
    20161 gattggcctt aggtttgtca cgctgggaaa cagtgataaa agtggtggtt ccagaagcga
    20221 caccagggat tttgactggt gtgatcctaa gttctggaag aatttttggt gaagcagctg
    20281 cattgatcta tacggctgga caaagtgcac ctgcgttaga ttttacgaac tggaatccat
    20341 taagtgtttc aagcccgatc agcattttcc gtcaagctga aacattagct gtccatattt
    20401 ggaagatcaa tacagaaggc acgatgcctg atggtgtttc tgtttcggct ggcgcttctg
    20461 ccgtgttgat tattgctgtt ttactgttca acttcggcgc tcgtgcgatt ggcaaacgat
    20521 tataccgaaa aatgacttca gcctaaggag aagaagcaaa atgaaaaaat ataactggaa
    20581 tgaaacaaat gtcatcacca tgccaaaaga gaaagaagta gcactatata cggatgatct
    20641 acatgtctgg tatggcgaca atgaagcaat caaaggagtg gatctggaat ttgaaaaaaa
    20701 taagatcact gcactgattg gtccttccgg ctgcggaaaa tctacgtatc ttcgttcgtt
    20761 gaatcgaatg aatgatggga tcgctaatac gaaaatcact gggaaaatca tgtatcaagg
    20821 cgtagatatt aatgcgcctc aagtggatgt ttatgagatg cgcaaacgga ttggcatggt
    20881 ttttcaacgt cctaatccgt tcagcaagtc gatttatgac aatattacat ttgctttgaa
    20941 acagcacggc gaaaaaaaca aaaagaaatt agatgaaatc gttgaaacaa gcttgaaaca
    21001 agctgccttg tgggaccaag taaaagacag cttggacaaa agtgccttgg ctttatctgg
    21061 cgggcaacag caacgtttat gtattgctcg tgcgatcgcg atgaaacctg atatcttgct
    21121 gctagacgag ccggcaagtg ctttggaccc tatttcgaca ggaacggttg aagaaacact
    21181 tgtaaacttg aaagaagact atacgatcat catcgtgacg cataacatgc aacaagcagc
    21241 tcgtatcagc gactatactg cctttttcta tttaggaaaa gtaatggaat atgacaaaac
    21301 taacaagata tttaccagac caaaaattca ggcaaccgaa gattatgtgt ccggtcattt
    21361 tggttaagac ggaggcctca acatgacaaa agagattatt tcatctaaag atcttcatct
    21421 gtattatgga aagaaagaag cactaaaagg gatcgatcta agcttcaatc aaggggaaat
    21481 cactgcgatg atcggtcctt ctggttgcgg aaaatccact tatctgcgtt ctttgaaccg
    21541 gatgaatgac ctgattccga atgtaacgat caccggaagt gttttatata aagacaaaga
    21601 catctatggt ccaaaaacag atgttgttga actaagaaaa gaaatcggca tggtattcca
    21661 gcagccaaac ccatttcctt tttctatcta tgaaaatgtc atctatggat tgaaattaaa
    21721 aggggaaaaa gataaaaaag tccttgatca agtagttgaa gaaagcctga aggcagcgtc
    21781 tgtttgggat gatgtgaaag acaaattgca taaaagtgct ttatctttat ccggtgggca
    21841 gcagcagcgt gtatgtatcg cacgtgtttt ggcagttaat ccggaaatca tcttattgga
    21901 tgaaccaaca agtgcgcttg atcctgtttc tactgggaaa atcgaaagta tgctgttaga
    21961 attaagagag cggtatacga tgatcatcgt gacccataat atgtctcaag cttcacgtat
    22021 ttcagataag acggcattct ttttagatgg tcacttgatc gaatacaatg atacgaagaa
    22081 gatattcatg aatccagaaa aacaagagac agaagattat atttcaggtc gatttggata
    22141 aggaggaaac ggaatgctac gtacacaatt tgaagaagaa ttgctgaatc tccataatca
    22201 gttctacgaa atggggatga tggtgagcag cgccgtgcac aagtcagttc gtgcatacat
    22261 caaacacgat aaaatcttag cccaagaagt tatcgaaaac gatataaata tcaataacat
    22321 ggaaacacgc ttggagaaga aaagttttga aatgatcgca ttgcaacagc cagttacaac
    22381 tgatttgcgt atgatcatta cggtcatgaa agctagctcg gatttagaac gtatggccga
    22441 tcatgcggtg tctgttgcta aatccacgat tcgcgtaaaa gggcagacga gaataccgga
    22501 aatcgaaaaa gagatctccg atatgtccga ttatgtgaaa aaaatggtgg acaacgtcct
    22561 agttgcttat gtcaaaacag acgaaaaaga cgcacgtacg atcgccaata tggatcaaag
    22621 agtcaatgac tatttcaatc gaatctataa tgcaacaatc aaaaatatgc aggaaaatcc
    22681 tgagacagtc atcagtggaa cagactactt gaatgttgcc agttatctag aacggatcgg
    22741 agactatgtg acaaacatct gtgaatggat cgtctacttg gctacaggga aaatcactga
    22801 gttaaatacg aaccacaatg agattttcta aactgaagct gtgattatgc cgtttgttta
    22861 ggaaaagagg ttgtgaaatc agcggttgtg aaaaagctgt tctgcatcaa gtaataagaa
    22921 cagtcaaaca aaaaatagct cctcatattt ttcgtttggc tggacttatt accgcagatg
    22981 caagcttttg aacaccgttt attcgggaaa gggtttgtga caaaacatgt cacaaaccct
    23041 tttcatcatt tcatagaaag gataaagaat gcaaaaaaaa attagcatca tcactatatt
    23101 ttttatttgt gtgcttactt catgggttat tcagcatcaa gccgctttat atgaagtacc
    23161 tgtcatcgag atagaaaaag tacgaataaa agaggatcat caattgatta caggaaaact
    23221 gatgaacacg gatcaaaaag gagaatcgat ttatttatcg gttccttatc aaaaaaatca
    23281 aatagaaaca gcaaatataa aagcgagaca aatgtatcta gtccaaatgc atggagatga
    23341 atggcagcta gtagcaaaaa aaaatgatgg ctggcttttc ttcttcacaa gtatcttcat
    23401 tggcgccatg ttgcttattg gtggaaaaca gggtctattt gctttaggaa gtctcatcat
    23461 caatatcgtt ttacttttac ttttgttgct catctttact aaaacagaag ggatttctct
    23521 attaaatata tctatcctgt tcgtcgttat agggatgatt attagcatat tggctttaga
    23581 tggatggaat agaagcagta taatcaaaat ttcagctgca gttacgagtg tttttttagc
    23641 attttttatt tgtcttttga caatgaatca ttggaaagac caaggactgc gctatgagga
    23701 actgaattat ctgacacgac cgtatcgttc tgtttttctt tctagcgtac tgattggaac
    23761 agctggagga acactcgata ccgttatcac agtcattgcg acattagaag aattgactaa
    23821 gcgacaacca gaaataacag cgaaacagtt atggctttca ggaaggcaag tgggtcgaga
    23881 tgtgattggc tcgatgacca atatcctttt gttcgtctat atcagtgggt cagttccatc
    23941 gcttctttta tatttaggaa atcaatggtc gttcaaagaa acgatagaac agcatttatc
    24001 attggaattt ttacgcgtca ttgctggcag cttaggaata gtattatcga tccctatctc
    24061 ggtcctcttt tttctgactg taaggaggct gaaacgatga atgtacttat tatacttgtt
    24121 gggattttag caatcagcgt gctacttatt ggcggtacgc aaggtatgta tattctttta
    24181 ggcttattca tcaatttagg gatctttttc ctgttgttat tcggttatca tcagaagtgg
    24241 ccgatactgg cattgtcggt cataggattt ttattgattg ctgtggttat cttatttttt
    24301 atcaacggtt ataatctaaa aatgcgtgca gcttttgtga gtatactgat ctttttattt
    24361 tgtttcctac tattgcttcc tatcactgat tttttagcaa tccaaggttt tacatcggta
    24421 gaactagaag aattatctgg cttagataag actttagcga ttgattttag attacttacg
    24481 agatcgttgc ttctgattag tcttagtggt gctgttttag atgcgagtgt agcaatttct
    24541 agtgggacat tcgaagtata tcaggcaaat ccgcaattat ctttttacca attgcgccat
    24601 tctagtttcg ctattgctaa aaaaatattg gcgtcaacag taatgacact tctttttgca
    24661 ttcatgggaa gttctttagc gttgatcctc tggtttattg atttagacat tccttttcag
    24721 caagtgatca atgaaaaatc atttgttcta gaatatacga tggctattct taccacattg
    24781 tccgcactac ttgttcttcc tttaacctgc gtgactacag catatctctt tacaaaaaag
    24841 aaagaacaac tcaagatgga gtaaaagtta caacttaagt cctatgacat ttttactgaa
    24901 tcagtgcata atatggatgt aaggaaaagc ttgcaaacaa ttttgcttaa ggaggcaatt
    24961 tccatgaaag aaagagaacg tattttagat ttagtgaaaa aaggaatcct ttcgactgaa
    25021 gaaggcttag atttattaga aagcatggca actgaaaaag acgaaaagca aatcaaaaaa
    25081 gaagcagaca aggtaactgc tagtcataaa gaaaaagatc aggcaagtca attgatcgat
    25141 aaacttgaaa acggcgaaga agaaatttct gagccagttg atccaaagga aagagaaaga
    25201 caagatcaag aaaacttaga aaagatctta gatgaactag caactgaagc gaataaaact
    25261 tcggcgcgtc tagatgaagt aaatgctcaa ttcgctgaca ataaggcagc acgcaatgag
    25321 aaacaggaag cattgatgca gctgaataca aaagaagaat taggcgagtt gacagaagaa
    25381 gaattagcac agcgtcagac gttagaagca gaaatcaaag aattagaagc tagtggcgat
    25441 acactgttag aagaacaatt gaaattagaa gcagaattaa aagatatccg taagaatcaa
    25501 tggtctgaga aaaaagaaac gtttactgat aagtttgaat tgccagatga ttggaaagat
    25561 caagcaacag atacactgaa tcaagtaggc gaaaaaatgt cagaagctgg atcacagtta
    25621 ggcaaattct tgaagaaaac attccaaact gtttcagaaa cagtgaatga caacatggaa
    25681 tggaaagatg tcagcttacg tgtccctggg attgcgacaa cgaaatttga gcatgaattt
    25741 tactatgaag ctcctgcagc aagtatcctt gatatcaagg cagcaaacgg aaacgtgacg
    25801 ttaaaaacgt gggacagcaa cgatgtgaaa gttgaagcta agatcaaact ttacggaaaa
    25861 atgggtgctg aaccatttga agcattctct gaacggagcc agattgaggt taatgaagat
    25921 catatctcct tccaaattcc aaataaacgt gtgcgtgcag accttgtttt ctacttacct
    25981 aaacgagtat acgaccacgc agctattaaa cttctaaatg gcaacatcat gattgaaaca
    26041 ttggaagcta aagatattta tacaaaatct acgaatggta acatcatcgt ggaccaatta
    26101 actgcaacga tgctggaagt agaaggtgta aacggcaata tcgatattcg aaacgggaac
    26161 atcttagatt ctattatcga aacagtaaat ggtacagtga catttggtgc gacagcagaa
    26221 aacctttctg tgtctttggt aaatggtgat gtacgtctta cgattaaaga agataatttg
    26281 aaaaaagtag aagcaagttc agtgaacgga aatgtcaaag tcgctttacc agatggcatc
    26341 ggtttggaag gacatgctaa aacaagtctt ggaagtatca acagccgtct gtccaattac
    26401 gaagtagttc gtgagaaaaa agaacggaca aaccaaatgt tgcaattccg tcgtgtttca
    26461 gatggtgaaa tcgcgcaagt gcaactttca acgacaacag gaagtatcta tttaaaagat
    26521 acagataaat aaagaggttg tgaaagagat gttcagcctt gagcaataag aagcgatttt
    26581 gtaaaatagc ttcccctatt ttacaaaatt gtgccttatt gccgatagtc tatctcttga
    26641 acaccgttta ccaagagatt atgaacctgc caattatcgc cgagtcacaa ggaataattt
    26701 cagaaaaagc tcgtcacgtt ttatgaatat ggtaatttgt gaccgagaat tttgcagaaa
    26761 gacaccgatt aagaagaagg ttgtcacaac gattttcttc atttaaaata gtagaacaga
    26821 ggtgagacat gatgaaaaaa aagttgacaa aatctaataa aaatgttgtt ttgacaggga
    26881 ctttagcagg aataggagaa tacatcggga ttgatccgac tgttatccgt gtgctttatg
    26941 tattcctgag cttagttgct tttggaagtc caattatttt atacatttta ttagctttga
    27001 ttatcccatc tgcgcgttct gcacgaacaa actatggaca taacaatgca tattatgaaa
    27061 aaaacaatta tcgtgaacaa actcgtgaac aaacaaaaaa acgcaaagaa gcggaaaaaa
    27121 tcgatgatga cgaatggagt gacttttaat gtcgtacttt caacgtattt tagttaatac
    27181 gttaacattc atatcattgg ctgttctttt tcctgcgatg gtccatgtga gcagtatttg
    27241 gacggccgtt cttgccagct tcgtattgtc tgtgttgaat atgtttgtca aaccgatttt
    27301 gacgatactg tccctgccct ttacattagt cacattggga ttgtttacgt ttgttatcaa
    27361 tgcagcgatt cttaaaatga cctccttttt tgtgggagca gaacatttta gtttttcttc
    27421 attcggtgca gcgttgttag tagctatctt gatgtcgctt gtcaatgtaa ttgtcagcga
    27481 acataacagt agaaaataaa agaaagctgt gccgaactat cggcgcagtt tttttattta
    27541 gaatcaggaa tgcgttttct attacattgg taattaaata aaaacgtttt ttttaattgg
    27601 tagtactttt ggctagacaa tgataaaatg atagagaaca ctctaataga aatgaggtag
    27661 catgaatgtc agaagttgta aaagttagcc aattagtgaa aaaattatct ttggaaatcg
    27721 tgacaggcga cgagaaaagc ttagaccgtg tcatcgtaac tggggacatc tcacgcccag
    27781 gactagagtt gactggttat tttaattatt attcccataa tcgtttacag ctatttggca
    27841 gtaaagaaat cagttttgcg gaacggatga tcccagaaga gcgtttgatc atcatgagaa
    27901 gattatgtag tgaagatatg cccgcattta ttatttcaag aggattacca gctccagaag
    27961 aaatgatcca ggcagcaaat gaaaatggga tagctgtact gcgttctccc atctcaactt
    28021 ctcgtttatt aggagaatta tccagttatt tagatggtcg ccttgcccct cgaacaagtg
    28081 tccacggtgt tttagtggat gtttacggat taggtgtact gattcaaggt gacagtggga
    28141 taggaaaaag tgaaacagcg ttggagttga tcaaacgcgg ccatcgattg atcgcagacg
    28201 atcgagtaga tgtttatcag caagatgaat taacggtgat cggagaaccg cctaaaatcc
    28261 tggaacatct gattgaaatc cgaggaatcg ggatcattga tgtaatgaat ctttttggtg
    28321 cgagtgcggt acgtggctct atgcaagtcc agttggctgt ttatctggaa gcatgggcaa
    28381 aagataagaa atacgaccgt ctgggctctg aagacacgac cgttgaaatc gcggaagtgg
    28441 gcattccaca aataaaaatc cctgtcaaaa caggacgaaa cgttgcaatc atcattgaag
    28501 tagccgcgat gaatttccgt gcacgtacaa tgggctttga cgcaacgaaa acatttgaag
    28561 aaagattaag tcgcttgatt gaagaaaact caggcaacga ctagcaagga gtgacaatat
    28621 gttagcacaa atcaatcgag ttgcttttga gcttgtaggg attcctatct actggtatgc
    28681 attgatcatc gtatcaggta tcattatcgc tatgtggctt agcagcagag aggctgttcg
    28741 ggtaggatta aaagcagatg atgtgacaga ttttatgttg tggggattgc ctatctccat
    28801 tattggcgct cgcttatatt atgttttatt tgatcttccc cagtatatag ctgatccaat
    28861 ccaaatattc aatatccgtt caggaggctt agctatttat ggcggactga ttgcaggcgc
    28921 gatcacgctg tattttttta ctaaatatca ttttatttct ttgtggactt ttttagatat
    28981 cgctgcacca agtgtattat tagcacaggc aatcggtcgt tggggaaatt tcatgaacca
    29041 cgaagcatat ggaccagaca cgacgcgaag cttcttagaa agtctgcatt taccgcaatt
    29101 catcattgat aatatgtata tcgaagggat ctatcgtcaa ccaacatttc tttatgaatc
    29161 actttggagt ttgattggat ttattttgct tttgttttta cgaaagaaac cacacctttt
    29221 gaaaaaaggc gaagtccttt tatgttatct aatgtggtat agtttcgggc gtttctttat
    29281 tgaaggatta cgtacagaca gtctttactt tttaggtgtg atccgtattt ctcaattatt
    29341 atctgctgtg ttatttgtcg gtacattgat tctatttatt tggcgcagaa aaaaaggaaa
    29401 tttaccggat tatgaccgct cattaggaaa aaatcaacaa attatctgat ttttagagcg
    29461 ttaaaaatga aaaaaatttc aggagtggta aaatgaatta tagagtagcg gttttaggac
    29521 caggctcttg gggtactgct ttggcacaag tcctcgccga aaacggacat gacgttcgga
    29581 tttggggaca tcatgcttca caagtagaag aaatcaacac ccaacacacg aatcaacgtt
    29641 atttaccaga tcattatcta cccgattcca tccaagcatt caccaatatg gaagatgcag
    29701 taaaagacgc agatgcggtg ctattcgtca ttccaacgaa agcaatacgt tctgttgcaa
    29761 aagaactagt gcctaaattg aatacaaagc cagtgatcat ccatgcaagt aaaggattag
    29821 aacagggaac acataaaagg atctctgaca tcttgttaga ggaaatccca gcagaaaaaa
    29881 gaaaagctgt tgttgttctt tctggaccaa gccatgcaga agaagtagca aaacatgaca
    29941 tcactacgat cacagctgca agtacagatg caaaagcagc aaaatatgta caagagctgt
    30001 tcatgaataa ttattttcgg atctatacca atcctgatgt gatcggtgta gagatggggg
    30061 cagctttgaa aaatattatt gctattggtg ctggcgctat tcacggctta gggtttggcg
    30121 ataatgcaaa ggcagcaatc atgacgcgcg gattagctga gatcagccgt ttaggtgtag
    30181 caatgggtgc caacccgctg acctttatcg gattaagcgg aataggcgat ctagtcgtta
    30241 cttgcacaag tgttcattct aggaactggc gcgcaggcaa cttgttagga caagggcagc
    30301 ccctagaaga ggtgctagcc aatatgggaa tggtcgttga aggtgtgaat actacccaag
    30361 cagcgatgga actagcccaa atgatggaaa tcgaaatgcc catcactcaa acaatctatg
    30421 aagtgctgta tgaacaaaaa gaaatcaaaa cagccgcaaa agaaatcatg ttacgtgatg
    30481 gaaaaaaaga aaatgaattc tcattaacaa ttttttgaag gagcgaatgt atatgaaagt
    30541 taaaaaagca gttattccag cagcaggatt aggaacaaga tttttaccag caacaaaagc
    30601 tatggcaaag gaaatgttgc caattgttga taaaccaaca atccagttca tcgttgaaga
    30661 agcgttgaaa tcaggtatcg aagacatctt gatcgttaca ggaaaagcta aacgaccaat
    30721 cgaagatcac tttgattcaa atattgaatt agaaacaaac ctaagtgaaa aaggaaagac
    30781 cgaattattg aaattagtag aagaaacgac agatgtcaac ttgcatttca tccgtcaatc
    30841 tcatccaaag ggtttaggac atgccgttct tcaagcaaga gcctttattg gaaatgaacc
    30901 atttgtcgtt atgctaggcg atgatataat ggaagacgaa gtaccattga cacagcaatt
    30961 gatcgatgac tatgagcgta cacacgcttc tacgattgcc gttatgcaag ttcctcacga
    31021 tgaaacttca aaatacggaa tcatcaaccc aggggaagta ctggaagatg ggttatacaa
    31081 tgtcaaaaac tttgttgaaa aaccagatcc aagcaaagca ccaagtgatt tagctattat
    31141 tgggcgttac ttattgactc cagaaatctt tgatgtatta tcagaacaac aaccaggtgc
    31201 tggaaatgag atccaactaa ctgacgcaat cgatacgttg aataaaacac aacgcgtatt
    31261 tgcccgtaaa ttcacaggta aacgttatga cgttggtgac aaatttggtt ttatgaaaac
    31321 aagtattgaa tatggtctga cacatccaga agtaggggca cctttgcgtg aatacatcat
    31381 gtctttaggt aaaacattag aagcagaagg aagcaagatc aatcataatc caaaaggaat
    31441 aacagaaatc aaagaagaaa agaaaaaata atcttttttt ctaacgagct tcagctgcta
    31501 attgaataaa aagtagttga agctcgtttt tatttgttta aaaaacacga aaatgctatc
    31561 cttatagaaa agagagacaa gaagggagtt tgatcatgtc aggaggagaa attgcaggat
    31621 tgatcgcagc aatcgccttt gcgatacttg tcgttttcat cgttcgcgta ttgttgcaag
    31681 tttccaaaac tttagaaaaa gtagaccaga ctgtcgcaga agcaaatacg acaattgaga
    31741 tcgtcacaca agacgtagat cttttatcta gacaagtaga aggcttgcta gtcaaaagta
    31801 atgagctgct cttggatatc aatggaaaag tcgcaacgat cgatccattg ttcacagcag
    31861 ttgctgattt aagtgaaagt gtatcggaac ttaatcattc tagtaaaaac atcgtgacga
    31921 aagtcggcgg aatcggaaaa acgacagcta aagcgactgt tgccggaaaa gtcggtggaa
    31981 cagcgatgaa attttttaaa aacaaaaaac atactgaaac gacaggagga aaataagaat
    32041 ggctaaaaaa ggtggatttt tattaggtgc agtagttgga ggtacagccg cagcaatcgc
    32101 cgcattattg ttagcaccca aatctggtaa agaacttcgg gatgaattgg ctgctcaagc
    32161 agatgatttg atggacatgg cttcagatta cacagatatg gcaaaagaaa aatcatctga
    32221 gttaacaggt attgcaaaag aaaaagcgca aggtctttct caacaagcag gggatctagc
    32281 aggcaatgtt aagcagaagg taagtgaatc agcagaggaa gcaggaagtg aatcagatga
    32341 tatcttgtta aacctcaaag aacaatcagc tgatttatct aatcgtttca aaaaaacagc
    32401 agaagatctg aaaaagccag cagacgaatt aggtagtatt gctaaagata cttcagaaga
    32461 tatcatgatc gatgtaaaag atgctgcaga taaagcagga gagacggtca atgaaggaat
    32521 gtcagaagca aaaccagttg cagatgacac aaaagaaatg ttgaaaatgg caaaagaaga
    32581 tgcaaaagaa gctaaaaaag aaatgaaaga tacaatgaca gattcgacta ccaatcacta
    32641 agattactca tttctagaat aaaaaaggtg agagcggacc agctctcacc ttttttattt
    32701 tcattactct aaaacaagta attcttttgg tgtagtcgtg aatggcacac aaccatcact
    32761 tgtcacatac acacaatctt cgatacgtac ccctactttt tcaggaatat agattcctgg
    32821 ttcaagagag aagcacatcc cttcttcaat cactaagtcg tttccttcta caagagaagg
    32881 gaattcgtgg acggttgttc cgattccgtg acctaaacgg tgattgaaat attcgccgta
    32941 gccatagctg ttgatcacgt ttcgtgcgat ttgatcaagt tcacctgctg tcacacctgg
    33001 gcgaactgcc tcttgtgcgg caagctgtgc ttcaagagtg atgttataga ttttttcttg
    33061 gaactcagtt ggtttttgat aagcaactgt acgagtagca tcactgcaat agccattcca
    33121 aataacgcca agatcgaata acactagttc atttggtgaa accttcgtgc ttcctggtgt
    33181 accatgaggg cttgcagcat ttgctcctgc aaggactaat gtgtcgaaag acatttgtga
    33241 aacaccttgt ttttttaatt ggtattcgat ttctgcgata attgcttgtt cagttacgcc
    33301 ttcttttact gttttgaagc caatttcaaa ggcaacatcg gcccatttac cagcttcgat
    33361 cagtttgtct ttttcttcta ccgttttgat cagttgcaga cgctggataa tagaagttag
    33421 atcggtgtaa gaatctgtac ctgtcaagat atcagagata gcatcgaagc gatcgacagt
    33481 cagagcattc ttttcaatac ctacattacc aggttgccca tagcgtgcgc gaagctcttt
    33541 tccgatgatc tcccaaggat tttcgctgtc taagtagcca cgtacatcgt acgcccagct
    33601 gctgttttga gcatcttcaa cttccagagc aggagtgaaa aggaaaggat cttgttccag
    33661 agggataaaa agagctagta cacgctcgtg aggatcactt tcatatccgg aaaagtaagc
    33721 gatgtggcca gggtcgctga tataagccaa ttctacttgg tgttgtgcca tccattgacg
    33781 tagttcattg atttttacgt tattcatttg gcaacctact ttctaaatta attttgtatt
    33841 caataattgt accactgatt gattaaaaga ccaacgttct taaaactgaa aaaagtaaaa
    33901 attttcatga aaacagagat aaaacggttg caattgttga gattatttga tatcctattt
    33961 tagaaatgta agacgtattt tcagaaaaca tataaaatat gaataggaga acacaaatgg
    34021 aaaagcaaac aatcactatc tatgatgtag cgagagaggc aaatgtttcg atggctactg
    34081 tttcacgtgt cgtaaatggc aatccaaacg tcaaaccagc aacaagaaaa aaagtcttgg
    34141 aagttatcga tcgcttggat tatcgtccaa atgcagtcgc acgtggcttg gcaagtaaga
    34201 aaacaacaac agttggggta atcattcctg atgtcagcaa tatgttcttt gcctctttag
    34261 cacgtggtat cgacgatgtg gcaacaatgt acaaatacaa tatcatctta gcaaactcag
    34321 atggcaacga tcaaaaagaa gtcaatgtat tgaataattt attggctaag caagtggacg
    34381 gcgtgatctt tatgggtcac catatcacag acgaaatccg tggagaattt tcacgttcaa
    34441 aaacgcctgt tgtactagct ggttccattg atccagatga acaagtaggt agcgtaaaca
    34501 ttgattatgt gtcagcaaca aaagatgcag taaataaatt agcaaaaagc ggcaataaaa
    34561 aaatcgcttt tgtcagcggt gcgctgattg atcctatcaa tggtcaaaac cgtctaaaag
    34621 gctataaaga agcattaagc gaaaacaatc taccatatag cgaaggattg atttttgagg
    34681 cacaatacaa ctttaaagat ggtctgtctc tagttgaccg gatccataat agcggtgcaa
    34741 cagctgtcta tgtttcagac gatgaattag ccattggtat cttggatggt ttattagacc
    34801 gcggcgtaaa agtaccagaa gaattcgaaa tcatcacaag caacaattct ttactgacag
    34861 aagtagctcg tcctcgtttg acaagtatca cacaaccatt atacgattta ggagcggtat
    34921 ccatgcgcct attgacgaaa atgatgaata aagaagaagt cgaagaaaaa acgattatct
    34981 tgccttatgg aatcgaagaa aaaggatcaa ctaaataata aaagatcaat catttaaagg
    35041 ttgttccaaa agtggtcaac ttctggaaca acgtttatac gagaaagggg gttatgacaa
    35101 aattttgtca taaccccttt ttttttgtga ctttaaaaaa agagaaaatg gcggaaacaa
    35161 accttacgtc catcgtatac gacacaatct gtgcgtagat gctacatttt gttcactact
    35221 atcaaaatta ctttaaaatg aaaacgtgat aaaaaagaaa gtagaaaagt agataagtaa
    35281 ctctaaaaaa tccaaaaaaa tccaggctcg attgcgtact tatcttaaag gagaaacaat
    35341 ccatcatata taacccgaaa ggagacttct atttgcctaa gaaaagacga aagaccatag
    35401 tcatcatttt gaccattctt actataggaa tcggagggaa attttacatg gacaaaagag
    35461 aaatgcaaag acagaaagat ttactcgcag tagaaaaaca gtccgtcaaa gttctgaaaa
    35521 atacctttgc ggatatcaag gaagtgaaga ttgaaaaatc tgctcgaaat gaaatgacgg
    35581 gttcataccg gatagttatt ctaatgacta ataaacaaga tcaatcgatt tatttttctt
    35641 atagtttttg gaaagaaaga aatgagatag gaagttacgg aatagttgat gaaaaaaagc
    35701 aaaaagaagg aaatacgctc aataaagtta aagtaactta ctctaatgga aatgaggaaa
    35761 gtatttaatg attactgaca aacaatatag tgaattaagt gatgctgttt actggctaga
    35821 tcctaaacat agagactatg tacctgaaat gcaagaaaat ttaagtttta aaattaacgg
    35881 cactatgtat aaaatcctaa aaatcaaaaa ctccttcgac ggcatgcagg cgatggctgt
    35941 agcgccaatc gtccatagca aattggagaa aaatttcaaa aataaaaaaa ttcctgcaaa
    36001 ctttcgcgta cttaaataat gaaggtgtca atacttacat aaaaagaaag gaggcacgtt
    36061 cttgtcaagg aaaataagaa aactcagctt agccatttta ttaatcgtca tattagggat
    36121 cggagggaaa gtctatatgg acaaaagaga agcacaaagg cagaaagatt tactcgcagt
    36181 agaaaaacag tcagtcaaag ttctgaaaaa tacctttgcg gatatcaggg aagtgaaggt
    36241 agaagaattt aaaaaaaacc ctgttaccgg aagttatggt gcacttgtga caatgacaaa
    36301 taatgaaggg aaatctgttt attttgatta tagtttttgg aaggagagaa atgaacttgg
    36361 tggttttggc attgagaaca gagcggtaca aaagacagga gtcactataa aaagagtaaa
    36421 agtaattttt tctaacggac aggaggagtt agtatgacaa taaacgataa gcattacaat
    36481 gatattagtg agaaggttta ttggctagat cccaaatatc cccgctacaa tgagggatac
    36541 aaaaagaact ctgttaaaga atttgctggt atggaatttc aaatcctaca aatcaaagac
    36601 tcattggacg gcatgcaggc gatgtctgta gcgccaatca agaacggtaa agtggatctt
    36661 tctcaagtag tgattgcttt tgcaggaacg aacttaagtg attggaaaga tttggaaacg
    36721 gatgcaagaa gtatcatgaa tctctacggg aatagaccag gagcaagttt ttctgaaaaa
    36781 gcaacaacaa acaagatttt atccgttagt ggattgaaaa cagcggggca aatcaattca
    36841 gcgattgcat ttgctaatga agtaaaaaag gaatatggag aagctgctat ttctactacc
    36901 ggccattcat taggtggatt cttagcatta tatgtagcag cagagaaaca atggaaaaat
    36961 gtcggcttca acgggccgga ctcgtatgat ctcttatcac cagaagcaaa aaaatgggtg
    37021 gaagaaaatc ccggtatgct caccaattac cgaaatcacg ccgattttat cggtaatttt
    37081 ggtggtaatg gcacaggtgc tgaagttcgg atcagtatgg aaacagcatt cttgaatctg
    37141 cttgcttccc acagtttaac ctcttggaag tttgatgaaa atggaaagct gattattcct
    37201 gaaaatgaaa acaatgttaa agctcgaaaa caacagaaag aaaaccaagc gacccagttg
    37261 ttttcctatc agttgcaaga actgaacaac atacgtaaaa aactacgatt aagtggcggc
    37321 cagctgacag ttaatgaaca aatttactta gatgacacac aagctaggtt agtagtggaa
    37381 aagttatcgg caaaaatgaa aagcgctatg gaagaagtta tccgcatcaa tcaaagagga
    37441 atttttgagc tagagaaaaa atggcaagaa ggcttgcggc aagcacgtgt tgtagcccct
    37501 tcgttgactg aacaggaaat catagaagca ttagctagtg tgggcgccac aaaagaaaac
    37561 ttagtcgatt ctgcgaaaga agtctatctg ctacgaatca ataaggcaag acgtatgggg
    37621 gaggcctttg atacattggc gaaggagatc caagacaaaa tcaatgaaat cgtggcacga
    37681 gatcgagaat tagcacagca attcaattaa aggagaagta gatgaaacta gaagaattgg
    37741 aaagagatta tcgaagtgaa cgaagacaat tagaagctca agaagatgaa ttgatccact
    37801 ttaagaatca aggattacga ttgatggagc aagaggagca gcgactgcat catgtattaa
    37861 agaacttagc tttagataat gagcctctgt ttcaagcaag acgaagttat tcgaattatc
    37921 aggagcatta tcaacaagcc ctacaagtag aacgacgaaa actctatcag cagcaagatg
    37981 aactagaacg ttcctatcgt caaaaaatca gacaaataac tatggagaaa tcatcttatg
    38041 aatgaatttt taatgcaatt actaaaagaa ttagagaaaa atctacatcg atcaaatgta
    38101 cgttcagttg gtatggggat atctcagcaa atttgggcag aaaaacgatt gacagatcaa
    38161 aactaccaaa tgactgcaga agaagctgct caagaatttt ctaaccgaca tgtacacttg
    38221 aaaggatcag taaaagggca atttgcagaa aaactaactg aattattcca aagacaagaa
    38281 aagaaattaa aacagtttcg ttagtaaagt tatatagaaa aattttgtga aaatggtt
//
LOCUS       DACDIE010000022        37667 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.022, whole genome shotgun sequence.
ACCESSION   DACDIE010000022 DACDIE010000000
VERSION     DACDIE010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37667
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.022"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            15..2180
                     /gene="acm"
                     /locus_tag="IV250_001229"
     CDS             15..2180
                     /gene="acm"
                     /locus_tag="IV250_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HAP8768523.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2452..2937)
                     /locus_tag="IV250_001230"
     CDS             complement(2452..2937)
                     /locus_tag="IV250_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAP8768524.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     gene            3417..4058
                     /locus_tag="IV250_001231"
     CDS             3417..4058
                     /locus_tag="IV250_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP8768525.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            4077..4712
                     /locus_tag="IV250_001232"
     CDS             4077..4712
                     /locus_tag="IV250_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP8768526.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4730..5545
                     /locus_tag="IV250_001233"
     CDS             4730..5545
                     /locus_tag="IV250_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8768527.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            5864..7300
                     /locus_tag="IV250_001234"
     CDS             5864..7300
                     /locus_tag="IV250_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8768528.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7489..9936
                     /locus_tag="IV250_001235"
     CDS             7489..9936
                     /locus_tag="IV250_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAP8768529.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            10166..11233
                     /gene="rlmN"
                     /locus_tag="IV250_001236"
     CDS             10166..11233
                     /gene="rlmN"
                     /locus_tag="IV250_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAP8768530.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(11426..12529)
                     /locus_tag="IV250_001237"
     CDS             complement(11426..12529)
                     /locus_tag="IV250_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP8768531.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            13082..13630
                     /gene="aac(6')"
                     /locus_tag="IV250_001238"
     CDS             13082..13630
                     /gene="aac(6')"
                     /locus_tag="IV250_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP8768532.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            14176..15837
                     /locus_tag="IV250_001239"
     CDS             14176..15837
                     /locus_tag="IV250_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8768533.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(15907..16254)
                     /locus_tag="IV250_001240"
     CDS             complement(15907..16254)
                     /locus_tag="IV250_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HAP8768534.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            16338..17279
                     /locus_tag="IV250_001241"
     CDS             16338..17279
                     /locus_tag="IV250_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8768535.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            17280..18323
                     /locus_tag="IV250_001242"
     CDS             17280..18323
                     /locus_tag="IV250_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8768536.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            18329..19399
                     /locus_tag="IV250_001243"
     CDS             18329..19399
                     /locus_tag="IV250_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8768537.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            19389..20342
                     /locus_tag="IV250_001244"
     CDS             19389..20342
                     /locus_tag="IV250_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8768538.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            20354..21364
                     /locus_tag="IV250_001245"
     CDS             20354..21364
                     /locus_tag="IV250_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8768539.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(21649..22470)
                     /locus_tag="IV250_001246"
     CDS             complement(21649..22470)
                     /locus_tag="IV250_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP8768540.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(22567..22845)
                     /locus_tag="IV250_001247"
     CDS             complement(22567..22845)
                     /locus_tag="IV250_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8768541.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            23113..25122
                     /gene="metG"
                     /locus_tag="IV250_001248"
     CDS             23113..25122
                     /gene="metG"
                     /locus_tag="IV250_001248"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAP8768542.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(25601..26188)
                     /locus_tag="IV250_001249"
     CDS             complement(25601..26188)
                     /locus_tag="IV250_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8768543.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            26363..27685
                     /locus_tag="IV250_001250"
     CDS             26363..27685
                     /locus_tag="IV250_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8768544.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            27840..28616
                     /locus_tag="IV250_001251"
     CDS             27840..28616
                     /locus_tag="IV250_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP8768545.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            28609..29178
                     /gene="rnmV"
                     /locus_tag="IV250_001252"
     CDS             28609..29178
                     /gene="rnmV"
                     /locus_tag="IV250_001252"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAP8768546.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            29171..30055
                     /gene="rsmA"
                     /locus_tag="IV250_001253"
     CDS             29171..30055
                     /gene="rsmA"
                     /locus_tag="IV250_001253"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAP8768547.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            30506..31135
                     /locus_tag="IV250_001254"
     CDS             30506..31135
                     /locus_tag="IV250_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8768548.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFGLIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(31259..32368)
                     /gene="ugpC"
                     /locus_tag="IV250_001255"
     CDS             complement(31259..32368)
                     /gene="ugpC"
                     /locus_tag="IV250_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAP8768549.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            32577..32999
                     /gene="mgsA"
                     /locus_tag="IV250_001256"
     CDS             32577..32999
                     /gene="mgsA"
                     /locus_tag="IV250_001256"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAP8768550.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            33322..33858
                     /locus_tag="IV250_001257"
     CDS             33322..33858
                     /locus_tag="IV250_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP8768551.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(33898..35661)
                     /locus_tag="IV250_001258"
     CDS             complement(33898..35661)
                     /locus_tag="IV250_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8768552.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(35664..37379)
                     /locus_tag="IV250_001259"
     CDS             complement(35664..37379)
                     /locus_tag="IV250_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8768553.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT        12428 a         6653 c         7752 g        10834 t
ORIGIN      
        1 gggagataga aaaaatgaaa aagtgtagaa atattatttt atcaatgtta tttattatta
       61 ctaatataac atcactgatt cctgttcatg tctatgcgga tgcaggcaga gatatcagca
      121 gtaatgtcac ttcgttgacc gtggacccaa cgaatattac agatggagga aacataaaag
      181 taaaattctc gtttgacgaa aaaaaacaaa atatccaacc aggtgattat ctttggatca
      241 attggccaag tgaaggaaat attcgcggtg aaggattcca aaaagaaatc ccattaatga
      301 ttgaaaataa aaatgttgga actttaacgg tcagaaaaga ttctgcgcag gttgtgttta
      361 atgaaaatat taaaaaccta gactcagtag aaggttgggg gcaatttgag atacaggcca
      421 gaaacgtaac cgataccggt gaagaaaata cgggaccatt caccgtgacg agcggtgata
      481 aaacagctac tgtaaatgtg acaaagccag cttctggttc ttcttctagc gttttttatt
      541 ataaaacagg agacatgcta cctgaagata ctaagcacat tcgatggttt ttgaatatta
      601 acaataacgg cacttatgtt gaacaaccag ttaaaatcag tgatgaaatc caaagtggac
      661 aaagattaga tccaagtacg tttgaaatta atcaaataca cttaggtgaa cagaaggttt
      721 atagaggaga agagggaatc caacaatttt tacaagattt ccccagcgct acatttaatt
      781 tttcagtaac tgataattac attgaaataa ctataccaaa aaactttgtc aatcttagga
      841 aaataatggt gagttataag acgatcatag aaaacccaga acaaataaat tttgagaatc
      901 attcggaagc ttggtttaaa gaatttaata aacctgctgt tgatggagaa agtttcaatc
      961 atacagttaa aaatatttca gcttctggtg gagtcaacgg tacagttaga ggcgaattga
     1021 agattttcaa atatatcaat gacacagaga ttggtatccc aaatgtgact ttcgaattaa
     1081 gacgagcaga tgaacagcct attcaaggac agtctagtat cctgttgact tcgaatgaac
     1141 agggagaagc tagtatcaaa ggcctgcaag taggagacta tgttgtaaaa gaaaaagaag
     1201 cacccaattg gattgatttt gatccactaa gttcaaatga actcaaattc tccataaacg
     1261 aaaatgatac agagggagtc agtttaccta tttataacaa gaaaaaggtt acgaacatca
     1321 cagcaactaa aatttggaac ggtggaacca ctccaagacc gagtatttat tttaaattat
     1381 tcagagcaag tacaaacaat tgggaaccag ttcctgatgc agaaacaaaa cgattagaca
     1441 atggaataac ttcagtgact tgggaagata tccaacagta tgatgatagt ggaaacgaat
     1501 atacgtttaa agtgcaagaa gtagatcaaa acggaaatga ttatgtccct tcaggttatc
     1561 aaaaaattga aaacgggcta gtcgttacaa atgagaataa agaagtcatt tcagtctcag
     1621 gacaaaaaac atgggaagac aatgagaacc aagacggcaa acgaccgacg acgatcacag
     1681 taaatctatt agccgacggt caaccgatcc aacacaaaga agtaagtgaa aaagatgatt
     1741 ggcgctatcg tttcacaaat ctgccaaaat acagagacgg gcaagaaatc atctatactg
     1801 taacggaaga caacgtgccc gaatacagta cgacgattga cgggtataac atcaaaaaca
     1861 gttatacacc ggaaaaaaca aatatatcta taccaggtag ccatataaca ccgtggaaaa
     1921 attttccaga gaagacagag agagagaaaa ttagaaatct attctctcaa aaattgcaaa
     1981 tatttgctca tacaggagaa tccagcgaac aaagaagcaa ttatattagt aagagagctg
     2041 tacctaaaca gataacaaat aaatcaaaaa gcattcttcc taaaacaagt agtaaaaaat
     2101 ctagttttgc tgtaatcatt ggtttattga tagtaactat ctgtgctggg atattcttac
     2161 aaaaatataa aaaagtttag ttagaatata atattttttg ttaaatatga ctgaaatcaa
     2221 aagaataaaa ttccgattta taatcgaaaa aagaagcatt tttatatatg cttctttttt
     2281 cgtagaatca ataaatatgg atttggctaa ctatggaaga atactcgtat tagttttcag
     2341 cgttcatact agttgttaca ttgtttgatt ggccaaaaga atttctgata tattttgtgc
     2401 taaataaaga aaaagacgct tgaattggga aataagaaaa ctactctatc attacaaatt
     2461 ttcataaact ggcagatgtt ctaagtaaac aagtatcttt tcaaagtcgg aaaaatccat
     2521 aatcaacgta gtggtattga tatttggatg aaagcctacg gtatcttgac gatcaatagc
     2581 agcatcgatc acgacttgga tttttttctc tttgtcgtgc atcaatgcta atggagtcac
     2641 tgtacctgct ggaacgccta ggaggtcagg aagctgttct tcagaaacaa aagataagcg
     2701 cttttctttt aattgttcag ctagttgttt taaatcggcg tgcttttcat caggcaagat
     2761 gacaagatag atttgttttc cttttcttga tttcaataat agatttttta cttgcggacc
     2821 aggtagggaa aaagggacat ttttgactga cgtaatcgca ggatgatcga ctttttgaaa
     2881 agagataccc agttttttta gtagttcata agcttcttgt tcggttgcac gactcatcgc
     2941 gcatcaccct ccttttttta ttagtgtatc ataaaaaaga gttgacaaat gaaaaataat
     3001 catgtaaatt acacgtaaag ttaaaacaaa tgacatcgaa aagaagagta gccgttaggt
     3061 gtttttagag agtctctggt tggtggaaag aggcagcagt taaccgtgaa acacatcttg
     3121 gagtcgattt tctgaagcgt atagtaggaa aatccgggga gcccgttaca gctgcggtcg
     3181 ttgctagatg accagataag gctggctttt ccgtgaggag tcggtaaaaa aaggtggaac
     3241 cacgtgatta acgtcctttg atcttcggat caaaggactt tttgttttca tctgaacggc
     3301 aacgactgtg tgaggttttt tctgcaagga aaaaataaac agaggtggaa ccacgtataa
     3361 tacgtcctct tggcatttgc caagaggatt tttttatttt aaaggaggaa aagaagatgg
     3421 agtatttgat tgaagttatg ccgcaattat tagtaggagc actcacaaca ttgaaattat
     3481 ttttatttac cttgattggc tcattaccat tagggattgt tttagcatta ggattgtctt
     3541 tgcattttaa gccgctacgc tacttgcttc aaacatatgt atgggttatg cggggaacac
     3601 cgctgttgtt gcaactgatt tttatttttt atgggttgcc gacgattggg atcgtttttg
     3661 atcgtttcga agcagcactc attgccttta tcttaaacta tgcagcttat tttgcagaga
     3721 tctttcgtgg agggatacaa tcgattcctc aaggtcaata tgaagcagcg caagttttgc
     3781 aattaagtcc ctttcaaacg gtgacgaaaa tcgtcatccc acaagtgaca aagattgttt
     3841 taccttctgt agggaatgaa gttatcaact tggtcaaaga ttcttccttg atctatatat
     3901 taggtttagg tgacgtaatg cgtgctggga aaatcgcgat ggaacgcgac gcgactttac
     3961 tgcctttgct aggcgtggca atcatctatc ttacattgac tggtattcta acaatcataa
     4021 tgaaacaagt tgaaggaaaa ttaaattatt accgctaact gataggagga agaaatatga
     4081 ttgaattgaa gaaagtgaac aaagcatttg gtgaaaaaaa aataatcaaa gatttgaatc
     4141 taatcattcc agatgggaaa acattagcta tcgtaggtcc ttccggtggc ggtaaaacca
     4201 cgcttttacg tatcttggct ggcttagaaa gttctgacag cggagaattt tatttagacg
     4261 gtgtaccgtt caacccattc gagcttaaaa tgaaagaaca agttgtagga gtcgtattcc
     4321 aagacttcca gctatttcca catctgaata tttttgaaaa cattacctta gcaccaaaaa
     4381 tggtattgaa acagacaaaa gaagaataca ccaaaaaagc agaagaatta gcggcttcat
     4441 tagggattag tgatttattg aatatctatc cttatcagct ttctggcgga caaaaacagc
     4501 gggtagcgat tgccagagca ttggctatga atccacgggt attggcttat gatgaaccaa
     4561 caagtgcttt agatcctgaa ttacgccagc aagtcgagcg tttgatccta tcattgaaag
     4621 aagatggggt aacccaaatt attgtcaccc atgatttagt atttgcagaa aatgtcggtg
     4681 atcaattact aaaagtttcc ccaattcatt aagaaaggag aagatagaga tgaaaaaaac
     4741 cagttttcta ttcgctttga tcgcaggtct tcttcttttt gcaggctgtt cgaacaaaaa
     4801 aacttcagca gataattggg cgactatcaa cgaagagaaa aaaatcgtta taggtttaga
     4861 cgatacattt gttcctatgg gatttagaga taaagacggg aagctcacag gatttgatat
     4921 tgatttagcc aaagcagtct ttgaacaata tgggattacg gtagactttc agcccattga
     4981 ctggtcaatg aaggaatttg aactgaacaa tggaacgatc gatttgatat ggaacggcta
     5041 ttccaaaacg ccggcaagag aaaagaaagt gcaatttaca aaaccttata tggaaaacga
     5101 tcaagtgctg atcacaccta aagaatcagg aatcacaaac tttggacaaa tgaataataa
     5161 gagattaggt gcgcaaaatg ggtcatctgg ttatgatgta tttacgaaac aaccagaagt
     5221 attaaaagag atagtcaaaa accaagatgc tgttttgtat gatagtttta atgaagcatt
     5281 gatcgatctg aaaagcagac gggtcgatgg tctgctgatg gataaagtat acgcagacta
     5341 ttatttgaaa caaagaaacg aactagaaga cttcaacata acaaaaggtt cttatgagag
     5401 tgaggatttt gcagttggtg cacgcaagtc agatagtaca ttagtgaaaa aaatcaatga
     5461 agccctagat caactagaga aaacaggtga attccaaaaa atatccgata aatggtttgg
     5521 cgaagatgtt acgccgagaa attaacaaat aggggactgt gacaaaagac gtgtcacagt
     5581 ccctttttcc gaataaacag tgttctcctg ccaactcctt gtgactcgga gctgaatggc
     5641 aggctcacaa cctcttggta aacggtgttc aaaagccagt ttttcggtat taagccaaat
     5701 ttatgcaaaa atgtagaaag ccattttcac aaaatttttc ttactactcg aaactaagcg
     5761 actttttcac aacctccttt acataatttt attttgttgt ggaaggggtt tcgtgttatt
     5821 ctaaaagagg aacgattgaa ggttatagga aaggaagaga tgaatggaac acaaagaact
     5881 tacctcattt ccaaaagatt ttctgtgggg ctctgcttct gcagcgtatc aagtagaagg
     5941 agcttggcaa gaagatggga aaggagaatc agtctgggat cgatttgtac ggatacctgg
     6001 aaagacattt aaaggaacga acggtgattt ggcagtagac cactatcatc gttataaaga
     6061 agatattgct ttaatgaaag aacaaggtct gaaagcttac cgcttctctg tagcttggac
     6121 acggattttt cctaatggac gaggagaagt caatcaagct gggctcgctt tttatgagag
     6181 attgatcgac gaattgattg aaaacgatat tgaacctatg ttgactttat atcattggga
     6241 tcttccacaa gcattgcaag atgaatataa tggttgggaa tcacgtcaga tcattgaaga
     6301 tttcactcat tatgcagaaa cattatttga ggcattccgc gggaaagtcc attactggat
     6361 tagtttgaat gaacaaaata tcttcacttc attaggttat ttgttagcag cacatccgcc
     6421 aggtgtgaca gacccgaaac gaatgtatga agttaaccat attgctaatt tagcaaatgc
     6481 atctgtcatc aataaatttc acgagttaga aatgcctgga aaaatcggac caagctttgc
     6541 gtatacgcca aattatccaa ttgacagtaa tcctgaaaat gttctggctg ctgaaaatgc
     6601 agaagacctg atggccaatt attggatgga tgtctatatg tggggaaaat atccgattgc
     6661 tgccatgagg ttcttagaag aaaaaggatg ggcaccaacg attgaagctg gcgatgcaga
     6721 attgctagaa tctgctaaac cagattttct tgggatcaat tactatcaaa cggcgacgaa
     6781 tgcattcaat ccattaaacg gagttggtgc tggaaaaatg aatacgacag gaaaaaaagg
     6841 ttcatcagaa gaaactggag ttcctggaat gtataaaaaa gttgagaatc catttgtcga
     6901 acgaacaaat tgggactggg agattgaccc agaaggatta aggatcggac tgcgtcgtat
     6961 cacaagccgt taccgcatgc cggtactgat tacggaaaat ggcttaggag agtatgacaa
     7021 gttgacagaa gataaacaaa tccatgacga ctatcgaatc aattacttac aaagccacgt
     7081 caaagccatc aaagaagcaa tttcggatgg agcagaagtc ctaggctatt gcacctggtc
     7141 ttatacagat ttgcttagct ggctgaacgg ttatcaaaag cgatatggat ttgtatatgt
     7201 tgatcaagac gaaacacaag aaggatcttt ggaacgatat aagaaagaca gttattactg
     7261 gtaccaaaaa ctgattgaag aaaatggaga gaacctttag ttcacatact taaaacaaga
     7321 aggtacggct ggatatggcc gtgccttttc ttgataaaat catttagaaa ttaagaaaaa
     7381 ctgtaaaata agctgaaaaa tgaaagaaag cgtcagttct ttcaaacaaa actttagttc
     7441 ccttactttt ttttgtataa tgaaacttga tgaaatgagg gataataaat ggcacaaaag
     7501 aaacggccta ctaaaaaaag gaaaaagacg aaaaaaacga aaaagcaaaa acagcagcag
     7561 gaacaaatgc tgacgattgg tgttgggttt gccttgattt tatttgctgt cttcggcttt
     7621 ttaaaattag gttttttagg aatattgatt gcaaacggct ttcggatcat tgcaggaaat
     7681 acttaccaaa tcctctgtct tttattagcc gtattaggat tttggattgt cattaagaat
     7741 acagagtttt caattggtaa aaaccgacgc tggtttggcg gcatactctt ttatttcggt
     7801 atcttattat tgttacatgc ccatttattt ggcaaactcc atactgggga accaaatatc
     7861 atggggacga catgggattt gttagccagt gatataaaac aaagtcaagt agacaataac
     7921 gttggtggtg ggatgattgg agcaatcctt tatcatttta cttacttctt gattgcacag
     7981 cctggaagtt atttagcagc agtgctatta ctggcaggag gagcattcct gttcagtaat
     8041 ctggaaggct atcagctgtt aaatggtatc caatcaattg gagaacgagt acaggaatta
     8101 ttggaaggtg atcctgccaa gcaagctcga aagcaagcgg caaaggaaga gagaatgaaa
     8161 cagcgcgcag aagcgaaaga agcaaggcgc ttagctgcaa aagaagctgc agaaaaagaa
     8221 gctgcagaat atgagaagaa aaaagcggtg agccagcaaa gggaaaatac tgctgatgaa
     8281 tggcaggaac cagaaaatac agagccggag cagctaagtt ttgttccaat cgacagtttt
     8341 caagaaaata tccatcctgc gaatctagag aaaccagtac cagatacacc aaaacagacc
     8401 aatacagcag aaggtttttc agatgagttg ccagaggatg acggtacctc attagaattt
     8461 gagattgaag cagaacagga aaatcaagac tatgaattac cgacagttga tttgcttgat
     8521 tctataccaa ctgtggatca aagtgatgag tataaaaaaa ttgaaaaaaa tatcggcgta
     8581 ttagaacaaa ctttccaaag tttcggtgtc gatgccaagg tcgtcaaagc cagcttagga
     8641 ccatccgtca caaaattcga agtccagccg gctgtagggg taaaagtaag taaaattgtg
     8701 aacttaacag atgatatcgc gcttgcttta gcggcgaaag atgtccggat ggaagcaccg
     8761 attccaggta aatcactgat tggtattgaa gtaccaaatg gaaagatcag tatggtttcc
     8821 tttagagaaa tcatcgaagc acaaccaaac cacccagata aattactaga agtaccttta
     8881 ggacgtgatg tttctggacg ggtacaaacg gctgatcttt cgaagatgcc tcacttactc
     8941 gtggctggtt cgactggtag tgggaagtca gtagcaatta atgggattat cacaagtata
     9001 ttaatgcgtg ctaagcccca tgaagtaaaa ctgatgatga tcgatccgaa aatggttgaa
     9061 ttgaatatgt acaacggcat acctcatctg ttgacgccgg tcgtgacgaa ccctagaaaa
     9121 gcagcgcaag ccttacaaaa agtagtacaa gaaatggaag aacgatatga aaaattcgcg
     9181 gcaaccggag taagaaatat ttctggttac aatgaatttg ttcaacagaa aaacttggaa
     9241 aacggaacga aacatccaac cttaccattc atcgtagtga tcgtagatga attagctgat
     9301 ttgatgatgg tagccagcaa tgaagttgaa gatgcgatta ttcgtttagc gcaaatggct
     9361 cgggcagcgg ggattcatat gatcctagct actcaacgtc caagtgtgga tgttatcaca
     9421 ggaatcatca aagcaaatgt accttccaga atggcatttg cggtatcaag cggtacagat
     9481 tctcggacga tcattgattc taatggggca gaaaagctct tagggcgagg agatatgcta
     9541 ttcttgccaa tgggtgagaa taaaccgatc cgtgtgcaag gagcattcat ttctgaccac
     9601 gaagtagaac gggtcgttca atttgttaca gatcagcaag aagctcatta tgaagagaaa
     9661 atgatgccga cagatgaagt tgaaacgaca ggtgctccag aacagccaca agatgagctg
     9721 tttgaagaag caaaagcact tgtcgttgaa atgcaaacgg caagtatttc ccttctccaa
     9781 agaagatttc ggatcggtta caaccgagca gcacgtttag tagatgaact ggaagctcat
     9841 ggagttgttg gcccgtcaga aggaagcaag cctcggaaag tattcattga gcaggaagaa
     9901 acatcagaaa cgttgccgga cgataatgac atgtaaaact tgtgtgatga agggttgaaa
     9961 aacgaataac caataaaaag acaaggcatg atgataccaa aagaaaaaat ggtttttttc
    10021 catcttttct aatacggtat gatgtcttgt ctttttagtt tttgtgagat taaagttcta
    10081 ttcgatttat ttgtgaagta ttgcattaag gaaatgaatc atgtatagta tgtagcgaaa
    10141 ctttgagaaa aagagggaaa atactttgga aaaacagtcc atttatggct tgacgaatga
    10201 agaattaatc aattggttta tagaaaacgg cgagaaaaaa ttccgagctg cccaagtttg
    10261 ggaatggttg tatcaaaaac gtgtaagtaa tttcacagaa atgacgaatc tatcgaaaca
    10321 attgatcgaa aaattatcgg agcattttat catcaatcct ctaaaacaga tggtcgtcca
    10381 agaagcttcg gatggtacag taaaatattt gtttgaactc cctgataaaa acatgattga
    10441 gactgtactg atgagacaag aatatggctt gtcagtttgt gtgacgacac aagtaggatg
    10501 taatatcggc tgtacgtttt gtgcaagcgg gctgctcaaa aagaaccgtg atctgactgc
    10561 tggagaaatt gttgcacaga tcatgatggt ccaacattat tttgatgagc gcaatcttgg
    10621 cgaacgcgtt tcacatgtcg ttgtaatggg gatcggtgag ccatttgata attacgacaa
    10681 tgtaatggac tttttgcata tcatcaatga tgccaaagga ttggcaattg gtgctcggca
    10741 cattacagtt tccactagtg gattagcaca caaaatcaag gaattcgcta ataacggctt
    10801 gcaagtcaat ctggctattt ccttgcacgc gccgaataat gaggtgcgaa catcgatcat
    10861 gcggatcaac cgaagtttcc caattgagaa attgatggaa gctgtggatg agtatttaga
    10921 gaaaacaaac cgtcgaatca cgtttgaata tatcatgttg aatcaagtaa atgatagacc
    10981 agagcatgcg caacaattag cagatctatt aaaagataag aaaaaattag catatgtcaa
    11041 cttgattcca tataatccag tcagtgaaca tgatcaatat tctcgcagtc caaaagctga
    11101 tgtattgaga ttttatgatg tattgaagaa aaacggcgta aactgtgtga tacgtaaaga
    11161 acatggtaca gatattgatg cagcttgcgg acaattgcga agcaagcaaa tgaaaaaaac
    11221 agttgccaaa taatcgtatc ctactcgttt tttcaattac cataaaagga gcagttttct
    11281 taattaagga actgttcaac ttaaataaga gaaaagattc tatagcgaaa gctataaagc
    11341 tttttttgac aagaaaaacc ctcatcaagc aaagaaatgt cctcaatttg atgacagcct
    11401 gcttgatggg ggttttagca attatttaac tgattttctt atatccttgc tggctttctt
    11461 gtgtagcatg ataagtttct acgtcaagaa tggcctgttt aaatttttct cgggtgattt
    11521 cataaggcac atgtttaata tcatttgttg ccatggcttt atctaagatt gcttctaaat
    11581 cggtcgtgac attgagattc atgtcggtta acgatactgg aagttggttc tttttcataa
    11641 acacatagat tttatcaaac aaatcgaact gctgatccat catcaataag gtcagtacac
    11701 cataactgac agatgctccg tgaaggtatc cctccatttt ttctagaaca gtcgtaccgt
    11761 aatagaatga gtgtgccata gcagaattat agttattgtt aactaaaaca gaaaccaatc
    11821 cggtagtaac aatgatatcc gaaacgactt gttcaattgc atatgaggga cgatcgtcgg
    11881 cactatcttt caatgcttgc gtaccataag tcaagagacg atcaatgcac atttttccga
    11941 tttggattcc taatctatcc gtgtagccta gatcatcgcc acgagcagaa aaagtagatt
    12001 cgacttcttt ggataaagca tcgccaatac ctgcccataa gtatttatca ggtgcttcgg
    12061 caataattgc tgtatcaatg aaggtgtgta caggtggttt tgaaggaaag aagagtccgt
    12121 ccattacgcc ttcttcgtta tagatgacac ataccgcagt gacgggcgaa cagttcgaac
    12181 caatagtggg aaaggtgaag actggtttag ctagtttttc accaatgact tttacagtat
    12241 cgcaagcacg gccaccaccg actgcaaaca ggacgtctgc tttttggaca ctttcatgct
    12301 ggatcaactg ttcaatattt ccataagttg aattaccccc gtaccagaga ctggcgacaa
    12361 cctctatttc ctgtgtcaaa gctactttta ttttgttttc tgcctttgaa agtgcagtct
    12421 tccctccaat cagcactact tttgtcccat attctttgac gattccattt gctttttcat
    12481 acgcatctgt tccaatggta taatttggta agaaaacact ttgactcata ttcatttcct
    12541 tctttcttcg ttgaaacggg ttaaaatttt aaagagtgat cgatctgact gcggctgtag
    12601 atggagtaga ggtgaaccta agacaggata agtgatgaat aaggtggcat gaacaccttg
    12661 aaatcatcaa taagtatcct aaaaacgaac aagtacaaaa acattgaaaa cgacaataat
    12721 gaatttttgt tcctcttagt atttctaccg attccctttg cttttttgta attatctaaa
    12781 aattctaaaa attaagatta ttatatgaaa acaggtttta tttgtcaatt cataattggt
    12841 tttttttaaa gcaaaagata atctatatca tgatgaacaa acagagtttt ttcctccaat
    12901 tgatggcttt cctgatcttt cctattttga ataaagtgtt tctgtataat ttttttattt
    12961 tataagtaag ataaataatg aaagaataga gatactgatt ggtagctaaa gatttttgag
    13021 cgagaacatt tcgttttttc aaaaggactg ttagaataag caagaacaag ggggaagacg
    13081 tatgataatc agtgaatttg accgtaataa tccagtattg aaagatcagc tttctgattt
    13141 actgagactg acttggccgg aagaatatgg agacagctcg gcagaagaag tagaagaaat
    13201 gatgaatcca gaacgaatcg cggtagcagc ggtagaccaa gatgagttag taggatttat
    13261 tggtgcaatc cctcaatacg gtatcacagg ttgggaattg catccattag ttgtagaaag
    13321 ctcccgacga aagaaccaaa taggtactcg attagtcaat tacttagaaa aagaagtagc
    13381 ttccagagga ggaatcacga tttatttagg tacggatgat ttagaccatg gaacaacgtt
    13441 aagtcaaacg gacctgtatg agcatacatt tgataaagtg gcttccatcc agaaccttcg
    13501 tgaacatccg tatgaattct atgaaaaatt aggttataaa atcgtaggtg tcttaccaaa
    13561 tgcaaatggc tgggacaaac cggatatttg gatggcaaaa acgattattc ctcgaccaaa
    13621 ttctcaataa taaaaagagg ttgtgacaga agtaatcagc ttcaagaaat aagaaggaat
    13681 ttacgaaaat tacttcttga atttttgtga aattccaact tatttccgaa gaagctactt
    13741 ttggaacacc gtgtaccaga ggttgtgaaa aagtcgctta gtttcgagta gtaagaaaaa
    13801 ttttgtgaaa atggctttct acatttttgc ataaatttgg cttaataccg agaaactggc
    13861 ttttgaacac cgtttattca gataagggac tgtgacaaga cttttgtcac agtttctttt
    13921 tgtttattct tttttatctt tgtatggcaa taatagacaa tataacgaaa attatttaat
    13981 ggcttttgga agaccatgga aaaccttgta caaagctatt ttttctgaaa aattccttga
    14041 aacctcacga aaaaatcatt attttatgaa aaagacaaaa aaaagtgttg attttttaat
    14101 aattattcat taaactctta aacaacttca gaaaatatta attattctga aaattccaat
    14161 aaaggggtac gtaaaatgaa aaagaaattt acatttggtt tggttgcggt atgcggcctt
    14221 gtacttgctg gatgttatgg cggcagttca gccacaagca gtagttcagc aaaaagcggt
    14281 tcagcagatg gaggaggcgt atttaactta gtcgtgcctc aagaaatgcc tacagctgac
    14341 ttatcagttg caacagatac aatcagtttt acagcattga ataacgttta tgaaggaatt
    14401 taccgtcttg atgaagacag caaaccacag ccggcaggag ctagcgagtt ggcagaagtc
    14461 agtgaagacg gattaactta caaaatcaaa ttacgagaag acgcaaaatg gtcaaatggt
    14521 gatcctgtta cagcagcgga ttacgtttat ggctggcaac gaacagtaga cccagctaca
    14581 gcttctgaat atgcttacct gtatgcgcca gttgaaaatg cagaagacat tacagctgga
    14641 aagaaagata aatcagaact agggatcaaa gcagttggtg actatgaatt agaaatcaaa
    14701 ttgacgaaac aaacaccata tttccaatat ctgcttgctt tcccatcatt tttcccacaa
    14761 aaacaatccg tagtggaaga aaatggggat gcctatgcat ctgcaagtga taaagctgtt
    14821 tataatggac cattcacatt agaagatttt gatggaccag gaacagatac agaatggact
    14881 tataagaaaa atgatcatta ctgggacaaa gatacagtaa aactttctga aatcaaagtc
    14941 agtgtagtaa aagaatcttc tactgcattg aacttattca aagatggcca agcagatgac
    15001 gtgatcttgt ctggtgagtt agcacagcaa aacgcaaacg atccagctta tacttctgtt
    15061 aaagaagcgc ggacaagcta tatcgaattc aatcaacgtg aaaagaattc accattcaac
    15121 aatgtgaacc taagaaaagc tatttcttat tctatcgatc gtgaagcttt agtaaaacaa
    15181 gtactaggtg acggatcagt tgtctctaca ggattgatcc catcagatat ggtaaaaagt
    15241 ccaacaacga acgaagactt tgcaaaagaa gctggaaaat tagtttctta tgacaaagat
    15301 aaagcaaaag aatattggga aaaagcgaaa aaagaattgg gcgttgattc tttagaattt
    15361 gatttgatgg cttctgatga tgattcatcg aaaaaagtaa tcgaatatgt tcaaaactca
    15421 atccaagaaa acttggatgg cgtaacagtc aaaccaacac ctgttccatt ctctgtacgt
    15481 cttgaccgct caacttcggg tgattttgac gcagtactag gcggatgggc tgctgactat
    15541 gcagacccaa gcagctttac tgatttgttt gtcacaggta attcatacaa ccgtggtcgc
    15601 tggagcaatg cagaatatga cgaagcagtt aaagcagctt catcaacaga cgctggcgat
    15661 gaacaagccc gttgggatga tttccaaaaa gcaatcaaga tcatctctga tgatatgggt
    15721 gtcgttccgg tgtaccaaaa agcagaaggt cacctagtga atgaaaaaat caaaggtatc
    15781 gttcaccatg cggcaggcgc ttcatgggat tacaaatgga catacgttga agaataatcg
    15841 ttaataccgt gagcatgaga aaaaacgagg ctgcggcaaa tgtcgcaacc tcgttctcat
    15901 attcacttat cctaacaaag aaaaaagcat gaaaatagca gaaagagcaa ttagtattcc
    15961 ggcaatgatc agccaaaagc tgtacatacc gtagctggca gacgagagcg aggaaaattc
    16021 tggttttttc tttcggttcc ttgttcgtgc aagatggacc gatcgctgaa aatgatcgaa
    16081 gaaacccgaa gaaaagaccc ataaaaaacc gccgataatc agaaacggca atgaccataa
    16141 gaatagatca ttagataatt gaatgagtgt aaaggaatgg ttgatgagat tttttattaa
    16201 aataaccaga atcagtagaa aagctactgc gtaggggata gatttttttt tcatttgatt
    16261 gctcctttat ttttggttca ttatctaata atgtagcgaa acatctgtca gaagtcaaaa
    16321 gctaggggga aacattagtg aatagttata tcaaatatgt attaaaacgt gtctttttca
    16381 tgatcatcac gctttggtta attgcaacaa tcaccttttt cttgatgcaa ttgctgccgg
    16441 gcacgccata tacgaatcag gaacgtttga gtcctgaaac aatcgagatg ctaaataaac
    16501 aagtcggttt ggataaacca gttatcgtac aatatgggat ttatctttct aatttgcttc
    16561 aaggagattt tggaatttcc ttccaattta aaaaccagcc agtcgcacat ttgttagctg
    16621 gaagaatcgg accatccttg cagttagggt tacaggcaat tatctttgga acagtcctag
    16681 ggacgatttt aggaacgatc tctgccatga agcaaaatac atgggcagat acatcttcta
    16741 cactcgtagc gatattaggt cgttccattc caaactttgt atttgctgtt ttattacaat
    16801 atatctttgc aatcaaatta caagttttgc caatcgcaaa atgggatggc tttgtgtaca
    16861 caatcttacc aacaattgcg ttggccatgt cgccgcttgc tgactctgct cggtttatcc
    16921 gtacggaaat ggtggaagta ctgcatagtg attatgtgga attagcaaaa gcaaaaggat
    16981 tgagccgttg ggaaatcgcc tttaaacatg gtcttcgaaa tagtttgatt ccattgatga
    17041 ctttattagg accgttagct gtagcattga tgaccggatc actggtggta gaaaatatct
    17101 ttgctattcc tggtatcggg gaacaattcg ttaagtcaat cacaacaaat gattatccta
    17161 cgatcatggc tgtaacgatc ctttactcat ttatgctgat ttttgttatt ttgatcgtgg
    17221 atctattgta cggtctagtt gatccaagaa ttcgtgtttc agaagggagc cgaggataaa
    17281 tggaaatgat accaaagcgt tatgaaacag ttgcagatat ccctgcggat gaatttcttc
    17341 cattacaaaa aaataccgaa gaagaacgtg aacagattac tgcaccttct ttgaatttta
    17401 tccaagattc atggcgccgt ttaaagaaaa acaaagctgc cgttatttca atggttttac
    17461 tattggtcat cattttgatc tctgtgatca caatctttgt ttctcctcat gatccaactg
    17521 cccaaaacgt ggcttatatc aatttgcctc ctcgtattcc gggaatcaat atcgatggat
    17581 tgaatggaaa agcaatggtt ggtggtgaac ttgttgataa atatgctcaa gcaaacgtcc
    17641 cttcaaacgt aaatttcttt ttaggaacag acggtttggg acgtgatgta ttaagccgct
    17701 tatttatggg aatgagaatc tcgttgctga tcgcatttat tgctgcatta ctagatgtaa
    17761 cgattggtgt agcttacggg ttggtttctg gtttgttagg cggacgtgtc gataacgtga
    17821 tgcaacgttt tttggaaatc ttatcaggaa ttccaaacct cgttgtcatg attttgatgt
    17881 tggtcgtttt tgaaccagga attttttcaa tcgtggctgc tatggccatc acaaactgga
    17941 taccaatggc acgtatcgtt cgtgcacaaa caatgaaatt aaaagatcaa gaatatgttt
    18001 tagctggatt gacattaggt gaatcaagaa tgaaaattgc attcaaacat attttgccga
    18061 atatctctag tgtcattatt attcaaatga tgtttagtat tccatctgct attttcttcg
    18121 aagccttttt aagctttatc ggtttaggtc ttacgcctcc gtcagcttca ttaggaacga
    18181 tgttgagcga agggtacaag acattcctgt atctgcctta tctattatgg attccggcag
    18241 taactttatc ggttatcatg attggcttta acttacttgc tgatggattg cgtgatgcct
    18301 ttgatcctaa aatgaaagag tgaagacgat gacgaaaatt ttagaagtga aagacttaga
    18361 aatctctttt gatacgtatg ctggaaaggt acgagcgatt cgcggtgtca attttcattt
    18421 gaataaagga gaaacattgg cgatcgttgg ggaatctgga agtggaaaat cagtgacgac
    18481 cagaagcatc atgcgcttgc tgtcaagcaa cgccaatata gatgctggtc aaatcctctt
    18541 taaaggtaac gatatcgttg gaaaatcaga aagagagatg caaaaaatcc gtggtaaaga
    18601 aatagcgatg attttccaag atccaatgac ctctttagat ccgacgatgc ccattgggaa
    18661 gcaagtcgct gagtccctta gaaagcataa taaagtttca aaaaaagagg gacaaaaagc
    18721 tgcattagaa ttgcttaaat tagttggaat acccgaagct gagaaacgta tcaacagtta
    18781 tccgcatcag ttctcaggag gtcagcgtca acggatcgtg atcgcgattg cactggtatg
    18841 ttatccggaa atcctgattg ctgatgaacc tacaacagcg cttgatgtga ctattcaagc
    18901 acagattttg gagctgctga aagaaattca gacaaaaatc gatacatcaa ttatctttat
    18961 cactcatgac cttggtgtag tagcgaatgt cgctgatcgt gtcgctgtca tgtatggagg
    19021 aagaatcgtt gaagttggaa catcggaaga aattttctac aacccgcaac atccctatac
    19081 ttggggactg cttggttcaa tgccgacatt agatagtgcg aacgatcgat tgtatgctat
    19141 tcctggttct ccaccggatt tattgaaccc gccaaaagga gacgcattct atccaagaaa
    19201 tgaattcgcg atgaaaatcg atgcagaaca agaaccacca ttttttgaac tatcaaaaac
    19261 acacaaggca gcaacttggc tacttgctcc gcaggcaccc aaagtgacac cgccagaaga
    19321 aatccaacgt cgctgggcta tctttaaaga gaaacaaaaa catacttacg gtcaaggagg
    19381 aattgctgat gccaaataaa ctacttgaag ttaaaggatt gaaacagtat ttcaatgtag
    19441 gaaaaaagaa tgaagtacat gctgtaaatg acatcagctt tcacatttat gaaggagaaa
    19501 cattcggttt agttggagaa tctggaagcg gaaaatccac tactggccgt acgatcattc
    19561 gcttgaatga acctactgat ggtgagatat tattcgacgg tcaagatata acgaagatca
    19621 aagataaaaa aggtttgacg aaattccggc atgatgttca gatgatcttt caagatccat
    19681 acgcttcgtt gaatcctcgt atgaaagtca gagatatcat tgccgaagga attgatgtta
    19741 atggactagc taaatcatct gaagagcgtg caaaaaaagt cgatgatcta ttgagaactg
    19801 ttggattgaa tccaagccac ggtactcgtt atcctcatga attttcaggt gggcaacgcc
    19861 agcgtattgg tattgcacga gcattggcag taaaaccgcg ttttatcatt tgtgatgaac
    19921 caatttctgc actagatgtg tcgatccaag cccaagtggt caatttgttg caagatctcc
    19981 aaaaagaaca tcaattgacc tatctattta ttgcacatga tttgtcgatg gttaaacata
    20041 ttagtgaccg aatcggtgta atgcataacg gcttgttatt agagatggga acaagtgatg
    20101 aaatctataa tcacggtgtt catccttata cagaaagttt gttatctgct attcctctac
    20161 cagatccaga ccatgagaga aaacgcagaa gaatcaaata tcaacctgaa ccagatgacg
    20221 gacaagtacg tcaacttcgt gaaatcgcac cagaacattt tgtctatgca actgagcagg
    20281 aagtcccata ttacgcaaaa aaactaaaac gacaaaagga atctcttctg gttgctaact
    20341 aggagggaca tagatgctaa aagcttttaa attccgtatg tatccgacag aagaacaaaa
    20401 acagcaattg attcgtactt ttggatgtgc tcgatttacc tataaccatt tgctgaaaaa
    20461 gcggcagaaa agttggcaac aaacaggagt agccaatttt tcgctcacac ccgctacatt
    20521 aaaaaaagaa tacccattct taaaagaagt agacagtctt gctttagcaa atgcacagct
    20581 taatttggat cgggcttttc gcaattattt caagggacgt gccagcttcc caaagcttaa
    20641 aacgaaaaaa agtatgtggc aatcttatac gaccaacaac caaacccgta cgatctatct
    20701 ggaaaatggc tacttaaagt taccaaaaca aaaagaacta atcaagataa atcagcatcg
    20761 gccagttgaa ggatcgattc gctccgctac gatttctgct cgatataatg aagaatttta
    20821 tgtcgcacta ctttgtgatg tttcgccagt aaaaaaagaa tctttagcta aatggatcgg
    20881 gatcgcctat catccgaaga cattgatcca aacatctcga cctctagaag taactttacc
    20941 aaaatttcac cagacagaag aaaagctgca acatgcacag cggaaattga atgtgaaagt
    21001 ccgctccgct caccatagaa agatcaggtt agatcaggca agcaattatc agaaacaaaa
    21061 gagaaaagta atggatcttt atctgaaaca aaagaaccag cgagaagatt atcttgaaca
    21121 attatctggg aaattggtca aacaatatga ctacttattt gtagaatctt ttccaaaaga
    21181 agaggcacat gctgattttt ctattcacga ctggcataaa ttaataacaa aattgcgtta
    21241 taagtcccaa tggtataata aaaaatttct tttgatcaat accgacggag cagaagaaag
    21301 caattccgta agaaagagcc aagtagtaga aaaaatgggc agacattcgg ttatcaaggg
    21361 ataaaaaatg aatgaaactg ataggagcgt tagggttagc cttgttaata aaaagtctta
    21421 atgacttttg tttctaggaa tttttctttt gaagaaaagc tcactaatta tgacaaacca
    21481 ataaaacaat agcagaaaaa gttcgtacga gctttttctg ctattgttaa ttttttattc
    21541 agaaagttta cgaatacatc aaaattctga aacaaaaaac gtaactcaca aagagttgtt
    21601 tttgcgtttt ttcttgaatg attaggtctg aatcttttgc tcacaacctt attcatggtg
    21661 ccccggcttg agctgataga tttttgaagg acggccgatt cctacaggac gttcaccgat
    21721 ctcatcgaaa tattggagca tagctttctt aaaattggaa tgatcgatcg ttttataatc
    21781 gatccctaag aatttagcaa atacttttcg agcttccgta atcgtgaaat ctttacctaa
    21841 aacttgtaga acttgcggct cgtgttccat tttattgact acacggttga atgctttcaa
    21901 gatgatttga ctatggtcaa aagccaaagt atctttgcca gaagactctc cagtgactaa
    21961 atcaagcgag atagccactt ctccgctaga taaatggatc ttgttactat gacgttctag
    22021 tgtgaaccag cgtacttcct tggcatcatc tccggcgatt agcggttctt ctccaataaa
    22081 ggctaagtag ctgactgtga cgacccagcc tcgcggatct cgattaggcg tactgaatgt
    22141 atgcagttgc tcaatatttt cttttgaaat aaccacacca gtttcttcct ttgtttctct
    22201 taagacgctt tctccagttg actcatcctt ttggacaaag ccaccaggca atgcccatga
    22261 atttcgataa ggatgccctt ttctttggat caacaaaact ttcagctgat cttcttcttt
    22321 gttatagcac atcagtacga tatcgacagt caaagagggc ttgtcatatt ctggacgttc
    22381 ctgtttttga taccagttaa gaaattcttc ttcacttgct ttatgttcat aatataattt
    22441 ttcttcttct ttagaagcaa aacggttcat ctgaccacct ccattgacag taaaagtact
    22501 ttttacctta ttgtagcaga ctctgaaaaa aaaccaagga tttacttgaa aaatctaggt
    22561 tatcatttaa atatgaaaaa taaatggaag tatgagattg aataaaaaga aaagcagata
    22621 aaacggcaat actttatacc gctcttcacg aatggcaaag aagccacaaa taacgatata
    22681 tacgtcaatg ataaaacgca aaatccaagc tgctgtcata aaactgttca aatgcccatg
    22741 gaggttaaaa taaccataca tacgaatata aaaatagctg aatattaaat gtgctaatga
    22801 aagaaaagca atactaagtg taaaaaaaag atgtttcttc tgcatgattg ttccagtccc
    22861 ttactaagat agatagtctt tattatacca cgtgtgaggg gagaaataaa aagccgtttt
    22921 ttagtagata tgaaacaaaa caaaaacccg catttattca acctattcat ccttttaaag
    22981 gatagattga acgtagatag aagtcgttta tccagtcttt ttcattttga aaaaaagcta
    23041 acagcatgct ttttttatgg tatactagca aggaaacgaa ttcgcatcat gcgataaagg
    23101 aggaagagcc ccgtggcaga aaaggaaact ttttatatca ctacgccaat ctattatcca
    23161 agcggcaaac ttcatatcgg caactcgtat actacgattg catgtgacgc aattgctcgt
    23221 tataaacggt tgatgggctt tgatgtgttt tatctcactg gagtggatga acatggtcaa
    23281 aaaatcgaaa caaaagcaga agaactaggt gtaaaaccgc aagaatatgt agataaaatg
    23341 gcggctgatg tcaagaaatt atggaaaacg cttgatatca gttatgataa attcattcgc
    23401 acgacagatg attaccataa agcagctgtt caaaaaatat ttgatcgttt attagaacag
    23461 ggagatattt acttaggtga atatgaaggc tggtactctg tttctgacga agaattcttt
    23521 acagaaacac aacttgcaga agtgtacaaa gatgacgaag gtaacgtgat cggtggtaaa
    23581 gcgccaagtg gacacgaagt agaattggta aaagaagaat catacttctt ccgcatgagc
    23641 aaatacgcag accgtctatt ggaatactat gaagaacatc ccgaattcat ccagccagaa
    23701 tctcgtaaaa acgaaatgat caacaacttt atcaaaccag gattagagga tcttgcagtt
    23761 tcacgaacaa cttttacttg gggagtacca gtcaagagtg atccgaaaca tgtggtgtat
    23821 gtatggatcg atgcattatc caattatatt actgcactag gatatggctc cgaagatgaa
    23881 agtctattcg aaaaatactg gcctgcagat gttcagatgg tcggtaaaga aatcgttcgc
    23941 tttcatacga tctattggcc aatcatgctg atggcccttg atttgcctct acctaaaaaa
    24001 gtatttgggc atggctggct attgatgaaa gacggaaaaa tgtctaaatc aaaaggaaat
    24061 gtcgtttatc ctgaaatgct tgtggaacgt tatggtttag atgcactccg ttattatttg
    24121 cttcgtgctg ttccgtttgg gtcagatggt gtctttacgc cagaagattt tgtttcccgt
    24181 gtcaattatg atctggcaaa tgatctaggg aacttattga atcgaacgat tgctatgatc
    24241 aacaaatatt gtgatggact cgttcctgac tatgcctcac ttgtcactcc atttgatagt
    24301 gaactgtcta cgacggctgc taacgttgtc ggacgttacc atgatgcaat ggaaaaaatg
    24361 gaattcaata cagcattggc tgaaatctgg gtgctgattt ctcgtgcaaa caaatacatt
    24421 gatgaaacag aaccatgggt attagcaaaa gacgaagaaa agaaagctga attgaatagt
    24481 gtcatgatcc atttagctga atctttgcgt atcgcagcga tattacttca gccaatcatg
    24541 acagaaacgc cagagaagat cttcaatcaa ctaggattgg actctgaaac aatgaatctt
    24601 gaaggattac actttggcga atttccttca ggaacaaaag tagttgcaaa aggtacacca
    24661 atcttcccac gtttagatat ggaagaagaa gttgctttta ttcaagaaaa aatgtctgaa
    24721 ggtactcaaa caaacgaaga tacagtcaaa tgggatccag aagaaacaga acttgtttca
    24781 acgaaagaaa aacaaatcaa atttgatgta tttgaaaaag tagaactaaa agtagcagaa
    24841 gtcatcaact gtcaaaaagt agaaggggca gataagttgc tgcaattccg actagatgct
    24901 ggagacagcc aagaccgtca aatcttgtca ggaatcgctg aattttatcc tgatccaagc
    24961 gaattgatcg ggaaaaaagt cgtcattgta gcaaatctga aaccacgaaa gatgcgtgga
    25021 caaatcagtc aaggaatgat cctttctgca gaagcaccag atggctcttt acaagtaatc
    25081 gaagcaccaa aatccatgcc aaatggctca gaaatcgcat agaaattcaa agggggtatg
    25141 acactagttg tgtcataccc cctttttccg aaatttgaga agcaattttc gtaaattcct
    25201 tcttattact tgaagccaat catataatac agaaacagag aactatccaa aataaatcct
    25261 agaaaaagaa gtacgagctt cgctgatttt tgttaattta gaataatact aataggaatc
    25321 gtaaaaacag aaaaaaagca taatgataaa gggtcgggca gattttaatt gattcatctg
    25381 gtactctttt ttattgtcct tacattcata aagtttgaat gcaatgactt tattacatct
    25441 atatttatat ctaacagtag cgttgatgtg ttaattataa gttctatcac tttatgttca
    25501 attagtttgt gctcttatat gtgtgattga aaatatagat aaaaaaacaa tcatcggctt
    25561 tgacttcgat gattgttttc tttattttgt aaaaatccta tcacttgatt ttatggtaac
    25621 cttttatttt tgtcttcaaa cggcttggta agagagcagt aagcagtgca aatgggatcg
    25681 aaaagacata ccagaaaatc gtacgtccga taccataatg ttgatagagc tttgtcaaca
    25741 tcacgtgtaa caagatcaac cctaaaagca aggagccagc tataaaccaa tgattatccc
    25801 aatgcatgac ttcactaagt gccatcactc caaaaggata gaaataaaaa tagatcccga
    25861 ctaaggtcat ccgccagttc tttgtaaatg ttccgatatt caataaaaag aaaatcgctg
    25921 aaagcaaaat accgataggc ggaaaaagcc caaccaaaaa ggaatacaac atcccccaca
    25981 agatcatcac aggacctttg atcaatgcaa cgattgcgat gaacaaagtc agcaatatta
    26041 gtttttgtga taacgttgca acagtcatta atccaaataa aattaatgtg aaccaatgcc
    26101 aatgggactg ctgttgttct ttacgggaaa aatcgacccg gttggataaa taatctgcta
    26161 atttcggata agaacgctta aaatccataa actgttcctc cttcgtggaa aaggtaggtt
    26221 tagtatacga aaaataaaaa agaaatacat ccgtcttaag agggagtttt tcactgaaag
    26281 tggagaaaaa agaatggtaa attaaaatat tctcagttat actgaaaatt ataccaaatt
    26341 tcaaagaaga aagaggatgg gaatgaaagg caaactaaaa cgagaaatca atctgtttgg
    26401 cgcattagct accgttatgg gaaccgtgat cggtgcaggc gtcttcttca aaactgctgc
    26461 tgttactgct agtactcagt ctacaagttt gacattgcta gcttggcttt tgggtggttt
    26521 cttaacgatc tgtgctggat tgacagtggc tgaattagct acagcaatcc cggaaactgg
    26581 tggagctgta aaatatattg aagctgcgta tggaaaattg cccagctttt tattaggatg
    26641 ggcgcaaagc ttgatttact ttcctgctaa tattgctgca ttatcgatta tctttgcgac
    26701 acaattgacg aacttattac aattatctac cgattatctt ttgttgattg cagtgatcac
    26761 cgctgtttct gtgacagggt tgaatttgtt agggacaaaa gtcggtgctt ctgtacaatc
    26821 agtgacgttg attgttaaat taataccaat tgctgtgatt gtcatatggg gattattgac
    26881 cccaggacaa ggaactgtcc agttatttcc aatcgaagct ggaaaagatg ttacttttgt
    26941 cgaaggattg agcagtgcgt tgttagcaac gttgtttgct tatgacggct ggttaggtgt
    27001 cggtgcaatg gcaggggaaa tgaaacggcc ggaaaaagat ttgcctaaag caattattct
    27061 tggtttgagt tttgtaacag tcgtttatct attgatcaat tttgtttttt tgaaaacttt
    27121 gccgattgat catcttgccg gtaatttgaa tgcagcttct gaagcatcag acgtcatttt
    27181 tggaggaatc ggtggaaagc tggtcactat tgggatcttg atctcggtat acggtgcatt
    27241 gaacggttat acactaactg ggatccgcgt gccttatgcg atggctttag aagacgagct
    27301 acctttcagt aaacagctga caaagctttc aaagaagttt acggttcctt atgttcctgc
    27361 ggttttccag ttagcagtag cttgtattat gatgagcctt ggttctttcg actttttaac
    27421 agacatgttg atttttgtga tgtggctatt cagtttactg atttttatcg gtgtctttat
    27481 tttaagaaaa aaagcgccgg aattgccacg tccatataaa gtaccacttt atccaatcgt
    27541 tccgattatt gcgattctcg gtgctatttt cattttaggt atgacgatga tgacgcaaac
    27601 gaaattagca ttgatcggta taggtgttac cttgataggt atcccagtct attatcaaaa
    27661 aaagaaaaaa cgctcagaag agtaaaatat ttcaacaaca gacagaaaaa gtacgaacta
    27721 ggtcctctag atcttcgtgc tttttctatt tcaataaaac aagttcttct attttgattt
    27781 ttgctacttc tgcaagtagt gcttatgtgc taaagtaaga atgaaaagga gagataaaca
    27841 tgatatttga ttctcatacc catttaaatg ctgaacagtt caatgaagat attccagaaa
    27901 cgattcagcg agcacaagaa atgggggtaa cgaaaatggc ggtcgtgggc ttcgatacgc
    27961 caacgattga aaagtctttg caattaagcc atgactatcc aaatatatac agtattatcg
    28021 gctggcatcc gacagaggct ggcagttata cgaaagaaat cgaaaataaa ttacaagaac
    28081 aattgacttt accaaaggtt gtagcattag gggaaattgg cttggattat cattggatgg
    28141 aagacccgaa agaagtccaa gataaagtat ttcgcagaca gattgctatc gcaaaagaaa
    28201 tgaacctacc aatcagtatt catactagag aagcacttga agacacgtat caaatcctaa
    28261 aagaagaaga tgttcgagat atcggtggaa tcatgcatag tttcagtggt gattatgaat
    28321 gggcaaaacg atttcttgac ttagggatgc atatttcatt tagcggtgtc gtcactttca
    28381 aaaaagcatt agacgtccaa gaggctgcag caaatgtacc aatcgaacga ttacttgtgg
    28441 aaacagatgc cccttatctg gcaccagttc cttttagagg aaagcgaaat gaaccaggct
    28501 atactcgtta tacagtagaa aagattgctg aacttcggaa tcttccgaca gaagaagtgg
    28561 ctgcccaaac atggacaaat gcgcaccgat tatttggtct aagcgaacat ggataagatg
    28621 aagatccaag agattatagt cgttgaagga aaagacgata caagaaggct gcgggaagta
    28681 gttgatgcag atacgataga aacaataggg tctgcaatca atgaagagat cctgatgcaa
    28741 attgaacatg cccaagagac tcgtggagtg atcgttttta ccgatcctga tttttcagga
    28801 gaaaaaataa gaaaaatcat tatggaagct gttcctcagg caaaacatgc ttttttgccg
    28861 cgcacacaag ctaaaccaaa gaaaaaggga agccttggtg tcgagcatgc aagtgatgct
    28921 gctattttag aagcattgaa aaaggtcgtg acgccagaca tttcccaaac ggctgtttct
    28981 gaaatcacta gacaagacct tttagactat ggtttaatag ctggggcaca agcaaaagaa
    29041 aaacgagaaa aactaggtga cgagttgcgc atcggttaca cgaacggcaa acaattggaa
    29101 aaacgtttga agatgtttcg tattacgccg aaagaactcg cccaagcaat gaaaatagtg
    29161 gaggaacaac atgagtgaat atcgtgaaat tgcgacaccc tctagaacaa aagagatttt
    29221 attgaaacat ggtttttcat ttaaaaaaag tttaggacaa aattttctga ctgaaccaaa
    29281 tattttaagg aaaatcgtag aaactgcgca aatagatgaa catacgaacg tgatcgaagt
    29341 tggaccagga atcggtgcat tgacggaaca attagccaag catgcaaaac aagtcgtagc
    29401 atttgagatc gatgatcgtt tgatccctgt attggctgat acgatgcaac cctatgacaa
    29461 tgtgactatt gtacatcaag atattttaaa gaccgatttg tcaacagctg ttagagaaac
    29521 atttcatgag gagcttccgt taaaagtcgt agcgaattta ccttactata ttacgacacc
    29581 gatcatgatg cattttcttg aatcagattt ggttgtggat gaactagtcg tcatgatgca
    29641 aaaagaagta gctgatcgta tctctgctga accaggaacg aaagcttatg gttcattatc
    29701 gattgctgtt caatattata tggaagccag tttagcattc atcgtaccaa aaacagtatt
    29761 tgtccctcag ccaaatgtag attcagcgat tttgaaactg acaagaagag atataccggc
    29821 agtcgaagtg acagacgaaa aagcattttt ccgattgaca aaagcggcat ttcagcaaag
    29881 aagaaaaacg ctttggaata acttgcagca ttcgtatggc aaagatgacc agacaaaagc
    29941 atggttagca aaaagcttgg aaacagctgg aatcgatcca aaacgtcgtg gggagacttt
    30001 gtcattacaa gaattcgccg cattaagcaa tgcgatgtca gaaaatatgc agtaaaaaaa
    30061 gaggttgtga cagaagttgt gaaaaagctg tcctgcatca agtaataaga acagccaaac
    30121 aaaaatagtt cctcatgttt ttcgtttgtt tgggcttatt accgcagatg caagcttttg
    30181 aacaccgttt aagaaagagg ttgtgggcct gccgtttaac tctaagtcac aaggaacaat
    30241 ttcaaaaaac agcttctcat gtttttgagt tagttggtct tattacttga tgcaagcttt
    30301 tgaccactat tatgccgaac aaggagatgt gacacaaatt ttgtcacatc tcctattttt
    30361 gtttcttgat caaaaaattc ttatacttga tactcacact cattttgtat ggatttagaa
    30421 aagaatatgt gtcttgacag ttattagtaa gtgagttata ctaagtcgac atttatgtgt
    30481 caagacatat taggagagag aaaaaatgag aaaaaattca gttcgaaaac taacgatttc
    30541 tgctttattg attgggatgg gggtcattat cccaatggtc atgcctaaaa tcatgattcc
    30601 accagcttcc tttactttgg cgtctcatgt gccattattt attgcgatgt tttttacacc
    30661 aggagttgca gtggctgttg cgctaggtac gacatttggt ttctttttaa caacgccggt
    30721 tatcatcgca ttgcgagcat tgtcacatct tgtgtttggg ttgattgggg cttggtactt
    30781 gcaaaagcat ccgagtatcg ttttaaaaaa tggacagttt acttttgcta attggcgttt
    30841 ccaagggttc aattttgtga tcggtgtgat tcattcttta gcagaaatgc tagtagtcag
    30901 catcttttac tttattggta atatgcctga aacatattat tctcaaggat atttttatac
    30961 agtattcatc ttgatgggga ttggtgggct tatccacagt ttagttgatt ataatattgc
    31021 ttattttata gcaggaacac tcagcaaata ttttgatatc cccgtattta cggcagcaaa
    31081 aaaagaaaat cggaaagagt cgattcaagg gtacgcgaag aaaacgacat attagacatg
    31141 atctctcaaa aaaaaaactc tcatctagta atggataata aatcctctaa gatgagagtt
    31201 ttttgtccta aaaagagctg caccaacatc ttctggcaca gctctaacag ggggcaattt
    31261 atggaagttt gatgacttgt tctgtttctt tatcaaagaa gtggcctttg ctgatgttga
    31321 aggcaaggtc gactatttcg cctggtttat ggaaatctct tgcatctact tttgagatga
    31381 actcagtaga accagttttt gtgtaaagca tcgtttcagc tccaagtaat tcggaaacga
    31441 caacttctga tttcactacg gcttcaggtg aagcttcaag ggctacttgt tcactgtgga
    31501 tgtcttctgg acgaataccg aagataactt cttttccttc ataacctttt tcaactaata
    31561 ctttgttttt tccttcagga atacgaagct taagcccatg tccatctgtg ataacaccat
    31621 ttttcaatgt cacattaaag aagttcatgg caggagatcc aataaagccg gcaacgaaca
    31681 tattgacggg tgtatcataa acttctttcg gtgtaccgat ttgttggata aatccgtctt
    31741 tcatgataac gattcggtca gccatagtca ttgcttctgt ctgatcgtga gtaacataga
    31801 tggtcgttgt ttcaagacgc tggtgaagtt tggcaatctc agcacgcatg gctacacgta
    31861 atttagcatc taaatttgat aatggctcat ccattaagaa gacttttgca tcccgaacga
    31921 ttgcccgtcc caatgccaca cgttggcgtt gtccaccaga taaagcagct ggttttcgtt
    31981 tcaagtattc tgtcagacct aagatctctg ctgcattgtc cacacgtttt ttgatgtctg
    32041 ctttatcata tttacgtaat ttcaagccaa aagccatatt atcaaaaaca gtcatatgag
    32101 gatacaatgc gtagttttgg aaaaccatgg caatatcacg gtctttaggt gccacgtcat
    32161 tcatgacttt tccgccgata tttagttctc cttcagagat atcttccagt cccgcgatca
    32221 ttcgcaaagt agttgacttt ccacaaccgg aaggaccaac aaaaacgata aattcacggt
    32281 ctttgattga tagattaaaa tcagttacag aataattttc tgcattgtca tattttttat
    32341 atacgttttt cagagccatt tctaccataa tacgttccac actttctttt ttagttttat
    32401 taactattta aagtataaat gaaagcgtta actaacttca atgtcatgtt gaacaagaat
    32461 tttttctttt ttttgtcaat gtgctagttt taagctttaa taaatatcca taagaaataa
    32521 aaactgatac actggagtaa ataagtatgt taacaatatc aaaaggagtg tctcttatga
    32581 aaatcgcttt gatcgctcat gaccgaaaaa aacctatgat ggtgaaatta gcaacagcgt
    32641 ataaaacaat attggaaaaa catgaacttt ttgctaccgg gacgactggg cagaggatca
    32701 cggaagcaac cggcttgccg atccatcggt ttaaatccgg accgttaggc ggggatcagc
    32761 aaattggagc catgatttcg gaggatcagt tagatttggt gatttttctt cgagacccgc
    32821 tggctgcaca gccccatgaa ccagatgtaa cagcacttat ccgtttatct gatgtctatg
    32881 agattccgtt agctacgaat atcggaacag ctgaggtgct actaagagga ctggaagccg
    32941 gcttcctaga ttttcgaaga atcgtccatg aaatagacaa gcatccactt tcattttgat
    33001 ggataagcgt atggaaaaat aacgtttaat gttcgcctat atgtagctat aaaagctaaa
    33061 caaactgata atatgcgata taatacgaac gtattggata atataaacaa taatattagt
    33121 ttttcgtatt tgcagtaaat gtgaaaatat gctatagttt ctgtgggaaa agatcccaaa
    33181 aataatacag agtagaaaat aaagcgttaa gtgctaagag gatgggatgt tgcctcttgg
    33241 acgaaggacg taatgttcgc ggttttattt tcgcattcgc tgcatgatac gatgtagcag
    33301 ctcttaaagg agatgctaaa aatgaagaaa caccggttag acgcacgtat gattgctatt
    33361 atgggcttat tgattgcatt gatggtcact ttgtcccgac tggtagcaat cgagacccca
    33421 tttattaaaa tcagtgtcac atttattcca caagtgatca tgggcattct ttttggtcct
    33481 ttttggtcag ggataggcgc agttttagct gatttagtag gaatggccct cttctcgaaa
    33541 tctgcattct ttattggatt tacattgaat gcatttattg aaggagcaat ctacggattc
    33601 tttttctatc gaaaagagat cacttggaaa aatgccattc tggctacgct tagtgtcacg
    33661 ctgatcatca acttattttt gacaccatta tggcttgcgt tgatgtacca tgtgccattg
    33721 ttcagctggg tcgtttgggc gccccgcctg ttaaaaacag taatatggtt accgattcaa
    33781 tcaattgcca tttattacgt aggacgtagt attccttaca aaaaaatttt aagaagttta
    33841 gcgatccacg ctaaataaaa aacaagcagt cttcccatcc cagaagactg cttgttttta
    33901 ttgtgctgtg aattgtgctt gatagagccg tcggtaataa ccatcttttt gaagcagctc
    33961 atcatgggtg cccatttcga caatagctcc ctgatccatc actaatatct ggtccgcatt
    34021 tttgattgtg gacaggcgat gggcaatcac aaaactggtt tttccttcca tcattgctaa
    34081 gaaggcttgt tggatttttt tctctgtcaa tgtatcgact gagctggtag cttcatctaa
    34141 gatcaacata gggggattac tgatcatcgt tcgagcaatc gtcaacagtt ggcgttgtcc
    34201 atctgatatc tttaaccctt gaccgccaat cttagtatct aatttttccg gcaaacgttc
    34261 tacaaattca tacatataag aggcttttag ggcatcattg atttctgcgt cacttgcttc
    34321 cggattccca aaacgaagat tttctcgcaa tgtactgtca aatagccaag tatcttgtaa
    34381 caccattcca aaacttttac gtaatgtatc acgttggata gagcggatgt catggtcatc
    34441 gactgtaatt gttccttttg ttgtgtcata aaaacgcatc agcaaattaa caagtgtgga
    34501 ttttccggcc cctgtttttc ctactatagc aatggtttga cctggataag cagtaaaatt
    34561 gaaatcttcg atcaaacgct gcttggggtc ataagaaaaa gaaacatgat caaaagagac
    34621 ttttcctttg atatttttgg gttccaaggc ataggcatct ggcaaatcag gcttttcttc
    34681 tggctgatcg atcaaatcaa atgctcgttc tagaccagaa aaagcggttt gcagttgtgt
    34741 cgtgattcca gaaagttcga taaatggttt agaaaactga cttgagtaga tcgtaaagct
    34801 ggaaatcaca ccaacagtga ttgacgaacc actcttcaat gccaataggc cgccaactaa
    34861 accaatcgat aaataagcaa ggtgatctac aaaacgagaa gacgggtttg tcaaggaaga
    34921 agaaaattgt gcccgttgtc ctttttcata tagatcagca tttagcagtt caaattgctg
    34981 ttgactagct tcttcttgct gaaaagcttt cacgattttc tggttaccga ttcgttcggt
    35041 cacaaagccc gagatatccc caataatcct ttgctgtcta tcgaaatttg tttgtgaagc
    35101 acgagcaacg atccaactca caaggaaaat aattggtgta gaacaaagaa cgaccaatgt
    35161 cagaagtgga cttagacgca tcataaaaat aaacgaaaga atgatgattg ccactccaga
    35221 aaataattga ttgaaggccg cagaaattgc aatggaaata ttgtccatat catttgtaaa
    35281 tcgactgacg atatttccgt gggagttttg atcataataa cgaagtggca atcgattcag
    35341 atgagcaaat gcatcttttc ttaattttgc aacggataag taagctaacc gattgcttaa
    35401 cacttggatg agccattgac tgactacgtt gattaaaacg ataccaccga atagaataag
    35461 taaatggaac agtcgattga agcggacctg tccttctcca atcatttgat cgatactttg
    35521 accaatttgt aacgtcatca caactgttgc aatcccattg atgattccta gtatcacagc
    35581 tagccaaatg tcttttgcat aggcagtaag gtaaggaatg aatcggcgga gcgtctgcaa
    35641 ggataatttt tttactttca taactattct tcctcctgtg atgcgacgat ttcctgatat
    35701 tctgctgatt gagcaagtaa ttcttcatgg gtaccctgag cagcaatctt accttgatcc
    35761 atcactaaga tatgatgtgc ttcttgaata gagcggacac gttgggagat aataatcact
    35821 gtactgccaa gataagtctg tagcgcctta cgcaaatcca agtctgtttg ataatccaac
    35881 gcacttaaag agtcatctaa aatcagtaat gctggcttgg caatcaaagc tcttgcaatc
    35941 gtcagtcgct gtttttgtcc gccagagaaa tttttcccat tttccatgac ttttgtatct
    36001 aaaccatttg gaagctgacg gacaaattct tctgcctgag cgattttcag agcttcccaa
    36061 cattcttttt ctgtggcgtc tttcttaccc cattgaaggt tttcacgaat agtcccggta
    36121 aatagtacag aagtttgggg aacaagagca atttgctgac ggagatgatc cagctgccaa
    36181 gaacgaacat ccattccgtc cattataact gtaccggaac tgacatcata gaaacgcgga
    36241 atcaattgtg tcaaagtcgt tttcccgctt ccggttggac caatgattcc aaggactgta
    36301 tttcgcttca gctgaaatgt aatattttgg agagaaagac cagaatcagg agtataacgg
    36361 aagtcaacat ggtcaaaagc gataattgct tcagaagaaa aatcgggagt agtactgacc
    36421 tcagcttcaa gaatcgaatt ttctgtgtct agcacctctt tcacccgatt tcctgaagct
    36481 tcggcacgtg tgaagatcac gacgaggttt gaaacaacga tcagtgccaa aagcatttga
    36541 ttcatataat tgatcaaagc aagaacttgt ccttgttgca agctaccaat atttactttg
    36601 atcccgccaa ctgtcagcaa acagatgatc ccgacattca tgatcaaggt tgtagcaggt
    36661 gataataggg cagagatatt ggaaacacgt aaataattgt tcgctagatc atttgtgctt
    36721 ttatcgaact tctctgtttc tttttttgtc tgcgcaaatg cgcggatgac acgaacaccg
    36781 ctaagattct gactaacgtg ttcattgagc tgatctagtt tttcttgcac tttttggtag
    36841 agaggaacgg tttttttgat gataaaaaat aaaatgattg agaaaatagg caataatgct
    36901 aagaaaaaca gacctacttc ccagtcgatc acaaaagcca tgaccactga cccgatactt
    36961 aaaaacggtg ctctgatcaa taaacgaatg accattgcta aagctagctg aagctgattg
    37021 atgtcgtttg tcattcgggt gatcaaggta tctgttccaa aatgattcaa ctcgttgtga
    37081 gagaaagtat tgattttttt gattagctga ttacgtaact ctgtaccaaa tccttgtgaa
    37141 gctactgaag catagtattg gcaaatgata gcacaaatca gaccaatcac agacatagcg
    37201 accatccata agcccatttc aatgattttt cctcggtcat tcatttttag tccattatcg
    37261 atcaaggaag ccatcattaa aggcaggaca agttcgaaga cagcttcaag aaatttgaag
    37321 aacgggccca aaatgatttg cttacgatag tcttttgcat aacgtaataa ctgaatcata
    37381 tgattcctcc tattatttat ttgtccacaa ttgaaaaaac aagtactacc acattgtagc
    37441 ttaggaaatt tagaatgaaa agagaacaag ccttttcata aatgaaagtt ttattatgaa
    37501 gaagctttag aaattgaata gactattttt tttaaaaaaa agaaaatgag aaaacatgcc
    37561 agtctcttca ttcataggaa cgtctatttt acttaatttt tctataaatg aattctgcat
    37621 aaaaaatata tcaagtaaat gtttatagat gaaactctat ttacttc
//
LOCUS       DACDIE010000023        37206 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.023, whole genome shotgun sequence.
ACCESSION   DACDIE010000023 DACDIE010000000
VERSION     DACDIE010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37206
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.023"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            4..816
                     /locus_tag="IV250_001260"
     CDS             4..816
                     /locus_tag="IV250_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAP8768554.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            813..1613
                     /locus_tag="IV250_001261"
     CDS             813..1613
                     /locus_tag="IV250_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAP8768555.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1774..3042
                     /gene="rseP"
                     /locus_tag="IV250_001262"
     CDS             1774..3042
                     /gene="rseP"
                     /locus_tag="IV250_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAP8768556.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3110..4819
                     /locus_tag="IV250_001263"
     CDS             3110..4819
                     /locus_tag="IV250_001263"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAP8768557.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5031..9383
                     /locus_tag="IV250_001264"
     CDS             5031..9383
                     /locus_tag="IV250_001264"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAP8768558.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            9527..10000
                     /gene="rimP"
                     /locus_tag="IV250_001265"
     CDS             9527..10000
                     /gene="rimP"
                     /locus_tag="IV250_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAP8768559.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10024..11199
                     /gene="nusA"
                     /locus_tag="IV250_001266"
     CDS             10024..11199
                     /gene="nusA"
                     /locus_tag="IV250_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAP8768560.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNSKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11221..11514
                     /locus_tag="IV250_001267"
     CDS             11221..11514
                     /locus_tag="IV250_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAP8768561.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11511..11822
                     /locus_tag="IV250_001268"
     CDS             11511..11822
                     /locus_tag="IV250_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HAP8768562.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11835..14141
                     /gene="infB"
                     /locus_tag="IV250_001269"
     CDS             11835..14141
                     /gene="infB"
                     /locus_tag="IV250_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAP8768563.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14168..14515
                     /gene="rbfA"
                     /locus_tag="IV250_001270"
     CDS             14168..14515
                     /gene="rbfA"
                     /locus_tag="IV250_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAP8768564.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14623..14844)
                     /locus_tag="IV250_001271"
     CDS             complement(14623..14844)
                     /locus_tag="IV250_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8768565.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYYRKKKVEFFTFLFLFFG"
     gene            15292..17655
                     /locus_tag="IV250_001272"
     CDS             15292..17655
                     /locus_tag="IV250_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HAP8768566.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKLGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRNDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFHSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAVTLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAKKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
     gene            18023..18946
                     /gene="truB"
                     /locus_tag="IV250_001273"
     CDS             18023..18946
                     /gene="truB"
                     /locus_tag="IV250_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAP8768567.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            18950..19891
                     /gene="ribF"
                     /locus_tag="IV250_001274"
     CDS             18950..19891
                     /gene="ribF"
                     /locus_tag="IV250_001274"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HAP8768568.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            20243..21028
                     /locus_tag="IV250_001275"
     CDS             20243..21028
                     /locus_tag="IV250_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8768569.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            21041..21892
                     /locus_tag="IV250_001276"
     CDS             21041..21892
                     /locus_tag="IV250_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAP8768570.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(22016..22468)
                     /locus_tag="IV250_001277"
     CDS             complement(22016..22468)
                     /locus_tag="IV250_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8768571.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(22933..23301)
                     /locus_tag="IV250_001278"
     CDS             complement(22933..23301)
                     /locus_tag="IV250_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HAP8768572.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            23451..23933
                     /locus_tag="IV250_001279"
     CDS             23451..23933
                     /locus_tag="IV250_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HAP8768573.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            23970..24980
                     /locus_tag="IV250_001280"
     CDS             23970..24980
                     /locus_tag="IV250_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAP8768574.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(25095..25808)
                     /locus_tag="IV250_001281"
     CDS             complement(25095..25808)
                     /locus_tag="IV250_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8768575.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(25998..26588)
                     /locus_tag="IV250_001282"
     CDS             complement(25998..26588)
                     /locus_tag="IV250_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAP8768576.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            27072..27299
                     /locus_tag="IV250_001283"
     CDS             27072..27299
                     /locus_tag="IV250_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAP8768577.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            27315..28481
                     /locus_tag="IV250_001284"
     CDS             27315..28481
                     /locus_tag="IV250_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAP8768578.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            28715..29758
                     /gene="hrcA"
                     /locus_tag="IV250_001285"
     CDS             28715..29758
                     /gene="hrcA"
                     /locus_tag="IV250_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HAP8768579.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            29852..30415
                     /gene="grpE"
                     /locus_tag="IV250_001286"
     CDS             29852..30415
                     /gene="grpE"
                     /locus_tag="IV250_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAP8768580.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            30466..32295
                     /gene="dnaK"
                     /locus_tag="IV250_001287"
     CDS             30466..32295
                     /gene="dnaK"
                     /locus_tag="IV250_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAP8768581.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            32446..33612
                     /gene="dnaJ"
                     /locus_tag="IV250_001288"
     CDS             32446..33612
                     /gene="dnaJ"
                     /locus_tag="IV250_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAP8768582.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(33694..34359)
                     /locus_tag="IV250_001289"
     CDS             complement(33694..34359)
                     /locus_tag="IV250_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar diacid utilization regulator"
                     /protein_id="HAP8768583.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            34335..34508
                     /locus_tag="IV250_001290"
     CDS             34335..34508
                     /locus_tag="IV250_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP8768584.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            complement(34736..34942)
                     /locus_tag="IV250_001291"
     CDS             complement(34736..34942)
                     /locus_tag="IV250_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8768585.1"
                     /translation="MICKASSGDEMAVKEILKFYDGYISKLCLRPFYHSESGKIIMQV
                     DEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(34968..35387)
                     /locus_tag="IV250_001292"
     CDS             complement(34968..35387)
                     /locus_tag="IV250_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009269612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP8768586.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            35891..36244
                     /locus_tag="IV250_001293"
     CDS             35891..36244
                     /locus_tag="IV250_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8768587.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            complement(36304..36471)
                     /locus_tag="IV250_001294"
     CDS             complement(36304..36471)
                     /locus_tag="IV250_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8768588.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(36590..>37206)
                     /locus_tag="IV250_001295"
     CDS             complement(36590..>37206)
                     /locus_tag="IV250_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8768589.1"
                     /translation="VPPNPFWASIGLSVSPLPLGSGMQYESSVSLGYLNQSFQNAVME
                     GIRYGCEQGLYGWNVTDCKICFKYGLYYSPVSTPADFRMLAPIVLEQVLKKAGTELLE
                     PYLSFKIYAPQEYLSRAYNDAPKYCANIVDTQLKNNEVILSGEIPARCIQEYRSDLTF
                     FTNGRSVCLTELKGYHVTTGEPVCQPRRPNSRIDKVRYMFNKIT"
BASE COUNT        12579 a         6467 c         7859 g        10301 t
ORIGIN      
        1 agaatgttac gtttttttcc gcaaaaaaac aaatatattc aagaaaaaag cgaatttcaa
       61 tttaataccg atggaccaat tcctcagcac attgccatca tcatggacgg caatggtcgt
      121 tgggcgcaaa accgtcgttt gccacgagtt gctggccata aagaaggcat ggaaacagta
      181 aagaaagtca caaaaaaagc atcaaggctt ggagtgaaag tgttgacgct ttatgccttt
      241 tcgactgaaa actggaagcg accaaaagat gaagtaagtt ttctcatgca actacctgtc
      301 gacttctttg atacgttcgt accagaattg attaaagaaa atgtcaaagt tcatgtcatg
      361 ggttatgaaa acgtattgcc agaacatacg caagatgctg taagacgtgc aattgagcaa
      421 acaaaaaaca atactggaat ggtattgaat tttgctttga attatggaag tagagcagag
      481 atcgtgactg ctgtccaaga aattgccgaa gaagtagcaa aaggagaaat acacgcagaa
      541 gaaatcgatg atgaattgat tgctaagcat ttaatgacag gtttcttgcc taaagagtta
      601 caagatcctg agctgatgat ccgtacaagt ggagaagagc gaattagtaa ttttctgtta
      661 tggcagatcg catatagtga gctgtatttt acaaaagcgc tttggcctga ttttgatggt
      721 gcgcatcttg aagaagcaat cgcctcttat caaaatcgag accgtcgatt cggcggtgta
      781 aaaaaaacag aagatgagaa aggggatcag tcatgaaaca acgtgtgatc acagcagctg
      841 tggcgctagt cttgtttatt ccgattatat ggatcggagg catagcggtt gagcttacag
      901 cagcaatctt agcggtagta ggagtttacg agctgttccg gatgaaagga ttgacactct
      961 taagttttga aggagtttta tccgccgttg gtgcagtgtt acttgttttg ccaaaagaaa
     1021 gatggttttt ctttcttcca gagaatgtaa gtacatttat gttattttat cttatagtta
     1081 tgattctttt aggaacatca gttatttcta aaaatacgta tacgattgat gaagcaggtt
     1141 ttcctgtgct tgtcagcttg tatgtgggaa taggtttcca gaattttgtc aatgcacgaa
     1201 cagaaagctt ggctgttttg atatttggtc tatttattgt ttgggcaact gatatcggag
     1261 catatatggt tggtcgacgt tatggtcagc ataagttaag tcctgatatt tcgcccaaca
     1321 aaacgattga aggtgcgctc ggtggtatcg gtagtgctgt tgtagtcgca tttctttatt
     1381 tattgctata cccagcaaaa gaactattca atcatggtat gtttgtcatg cttctgatga
     1441 cgattatttt ttcagcagtc ggacaatttg gtgatttggt cgaatctgca atcaaacgtc
     1501 attatgatgt gaaagactcg ggaaatatct taccaggcca tggtggaatc ttagaccgtt
     1561 tcgacagttt gctgtttgta tttcctatca tgcatttatt tggcattatt tagtaagtat
     1621 acctgcgttt ctttgtgaat ttcataaaag acgcaggttt ttatttttca ttatattgat
     1681 actattggtg tctttggtat actatggtaa aattatagtg ttttatcgtt gttttataaa
     1741 aaacacaaaa aacaagtaaa ggagaaaaaa gctatgaaaa cgattctgac atttatcatc
     1801 gtttttggta tattagtgat tgttcatgag tttggtcatt tcttctttgc gaaacgatca
     1861 ggaatcctcg tccgcgaatt tgctatcggt atgggtccga aaatttatgg gcatcaagca
     1921 aaagacggga caacatatac gcttcgccta ttacctattg gcggttatgt tcgaatggca
     1981 ggcaatgggg atgatgaaac agaaatggca ccaggaatgc ctctttctct tttactaaat
     2041 tctgacggca tcgtagagaa gataaattta agtaagaaaa tccaattgac aaatgctatc
     2101 ccgatggaat tgagccgtta tgatttagaa gatgagctga ctatcactgg ttatgtgaat
     2161 ggagacgaaa cagaagtagt aacctaccct gttgatcacg atgcgacgat tattgaaaac
     2221 gacggaacag aaatcagaat tgcgccaaaa gacgtacagt tccaatcggc taaattgtgg
     2281 cagcgtatgc tgaccaactt tgctggaccc atgaataact ttattttggc aatcgtgtta
     2341 ttcatcattc tggcatttat gcaaggtggt gttcaagtga cgaacactaa tcgcgtagga
     2401 gagatcatgc ctaatggagc tgcggcagaa gcaggcttaa aagaaaatga tgaggtagtc
     2461 agtgtggacg gaaaagaaat ccattcatgg aatgatctga caacagtcat tacgaaaaat
     2521 ccaggtaaaa cattagactt taagatcgaa cgagaaggtc aggtgcaatc tgtcgacgtg
     2581 acaccgaaat ctgtagagtc aaatggcgaa aaagttggcc aacttggtat aaaagcccca
     2641 atgaatacag gttttatgga taagatcatt ggagggacgc gtcaagcttt cagcggttca
     2701 ttggaaattt tcaaagcctt aggatcacta tttacaggtt tcagtttaga taaattaggc
     2761 ggacctgtca tgatgtacca attgtcttca gaagcagcaa atcaaggtgt aacaactgtt
     2821 attagtttga tggcgcttct ttcaatgaat ctcggaatcg tcaatctgct tccgattcct
     2881 gccttagatg gcgggaaatt agtcttgaat atttttgaag ggattcgcgg gaaaccatta
     2941 agtcaagaaa aagaaggaat attgacatta gctggctttg gatttttaat gttgttgatg
     3001 gtgctagtta catggaacga tattcaacga ttctttttct agaaaaagtt gacaaatagg
     3061 gtataataac aaacgataac tattttgagg aaataaagga gtatatagaa tgagacaatc
     3121 aaaaatgttg attccaacat tacgcgaagt tccaaatgat gcagaagtat taagccatca
     3181 aatcttgcta agagctggat atatccgcca agtatcagcg ggtatttatt catatctgcc
     3241 attagcaaat cgtgtattgg aaaaactgaa acgcattatg cgggaagaat ttgaaaaaat
     3301 cggagcggta gaaatgctga tgcctgccat cttgccagca gaactttggg aagaatccgg
     3361 gcgttacgaa acatatggac caaacttgta tcgtttcaaa gaccgcaatg atcgaaaaat
     3421 gatccttgga ccaactcacg aagaaacttt tactgatttg atgcgtaatg aaatcaattc
     3481 atataagaaa ttacctttaa atctttatca aatccaacct aaatatcgtg acgaaaaacg
     3541 acctcgcttc ggtttgcttc gcggaagaga gtttatcatg aaagatgcat attctttcca
     3601 tgcaacggaa gaaagtttgg acgagacata taaagattac gaaaaagcct ataccgaagt
     3661 attcaagcgt tgtggcttag aatttcgctc gatcatcgga gacggcggcg caatgggcgg
     3721 gaaagactca aaggaattca tggcgatctc agaaatcggg gaggatacga tctgttattc
     3781 gactgaaagt gattatgctg caaacttgga gatggctaca agctactatg tatcaaaaaa
     3841 atcgcatgaa acacagcttg aattggagaa agtagctaca cctaacgtaa aatcaattga
     3901 cgaagtagca gatttctttt ctgttgaacc tcaaaaaatc atcaagtctg tattgttcat
     3961 tgcggatgaa caacctgtcc ttgttttggt acgtggagac catgaagtca atgacgtaaa
     4021 actgaaaaac tttttatctg ctgacttttt agaagaagca acagaagaag aagcacaaaa
     4081 atatctaggt gctgagtttg gttctgtcgg accagtcggt gtttcagatg acgtaaaagt
     4141 atatgcagac cgttatgtcc aagatttagc gaatgccatt actggagcga acgaaacagg
     4201 attccactat aaaaatgtga atccagaacg cgatttcact gtgctttctt atgaagatct
     4261 tcgttttgta caagaaggag atccttcacc agacaacaac ggtgttcttg cctttactcg
     4321 aggaatcgaa atcggacata tattcaaaat cggcactcgt tacagtgaat ctatgggagc
     4381 aactgtacta gacgaaaatg gacgagaaaa atttgtgatt atgggctgtt atggtatcgg
     4441 tgtaagtcgt ttattatcag caatcgtaga acaacattca gatgaacaag gaatcaattg
     4501 gccaaaagga attgcgccat ttgatctgca tatcgtccaa atgaatctaa aagacgaata
     4561 ccaaacaagc ctgacgaatg aactagaaga agcgatgaca aaagctggct atgaagtact
     4621 ggttgatgac agaaatgaac gcgcaggagt gaaattcgcg gattctgatt tgattggctg
     4681 tccaatcaga atcactgtcg gtaaaaaagc agtggatgga atcgtagaag tgaaaatcaa
     4741 gaaaactggc gaaatggttg aagtacgtaa agaagaattg gcgaatactt taccaatcct
     4801 cttgaaccaa ggcgaataat aagcgttaaa acacagaaaa taaaaatagc tgaatcagct
     4861 gtttttgaca atctgatgat tcgcttttaa aattggaact gcgatttgaa tcgcagttcc
     4921 aattttttaa tgtaaaactc agaaaaaacg agtatactaa aaggtactga aaaagaagag
     4981 gataagatgc gatcaattgc attgtttctc atcagaaagg gcgtatatct ttgtcagaaa
     5041 aacgagattt atttgaaaaa ctgttggaac aaatccaact agaggaagca gaaaaaaatc
     5101 atccactgct tactgctggt gaaatggata gtgtagtggt ccatcgtaaa tcgcgtttat
     5161 gggaatttac gctacatttt tccaaaattt tgcctattat gctatatcgg tcattgactc
     5221 agcatctgac tatcgctttt aaagatattg cacaagtcaa actgaatatc ttagcagatg
     5281 accagacatt cgatgaacag ctcttgcaag attactgggc ccaagctttg gaaaatcagc
     5341 aatgcgatac accactagca cagcaagtat tgaaaacgca ggtacctgtg ataaaagatc
     5401 gtaaagtcat cttgccaatc gacagtacag gagctatttc ttatttgaag caacaatact
     5461 tgccattagt agaagagttg ttcgtcagtt acggattccc aaaatttcgt atcgaaccag
     5521 aagtggatga gcagcaagcg gaacgtgtat tgaaactttt tgaagaacgc aaacaagaac
     5581 aggcagaagc ctttatgaag caagcagctg aaagtctagt tgtccacgaa caaaagaaaa
     5641 aggaaagaaa agagcaatcg ccagctcttg aaggacccat ccagctagga cgaaatattc
     5701 cagccgatga accaacgaca ccaatgatca gtatcgttga agaagaacga agagtcacac
     5761 ttgaggggta cgtattcgac aaagaagtca gagaactacg ttcaaaacgc aagatcttga
     5821 ctttgaaaat cacagattat acttcatcat ttatcgtcaa aaaattctcg aacggcgaaa
     5881 aagatgaaca agtatttgat gcgatcagtg tcggtagctg gttaaaggta cgaggaagta
     5941 ttcaagaaga tacgtttgtc cgtgatttgg tcatgaatgc gcaagatatc atagaagtca
     6001 aacatacacc tcgcaaagat tatgctcctg aaggggaaaa acgagtagag cttcatgttc
     6061 atagcaatat gagtacgatg gatgcaacaa acagcatctc tgatcttgtg gcacaagcag
     6121 gaaaatgggg acaccgagct atcgccatta cagaccatgg tggtgcgcaa gcctttccgg
     6181 aagcacatag tgctggaaaa aaagcaggtg tgaaaatctt atacggcgtg gaagcgaatg
     6241 tcgtagatga tggtgtccca attgcttata atgatgcaca tgaagcattg agtgaagcca
     6301 catacgtcgt atttgacgtg gaaacgacag gattgtctgc agtatatgac acgatcatcg
     6361 agctagccgc agtcaaaatg tataaaggaa atgtgatcga aagctttgat gaatttatcg
     6421 atccaggaca tccattgtca cgaacaacgg tagatctgac tggtatcacg gatgaaatgg
     6481 ttcgagggtc aaagtcggaa gaagaagtat tgcggatgtt ccttgaattt tcaaaagata
     6541 caattttggt agcccacaac gcggcttttg atatggggtt cttgaatact agctacgcaa
     6601 ggtatggtat tcctgaagcg gccaatccag tcattgatac gttggaatta gctagatatc
     6661 tgtatcctca attcaaacgt ttcggattag gtgtgttatc taaaaagttt ggtgttagct
     6721 tggaacagca tcaccgagca atctatgatg ctgaagcaac tggtcactta gcatggatct
     6781 ttgtgaaaga ggcaatggac aatcataaca tgctttatca tgatcagcta aatgagcata
     6841 tcggagaagg agattcttac aaaagggctc gacctttcca tgtaacgatt ttagcaaaaa
     6901 atcaggcagg actgaaagat ttgttcaaac tgatttcaat gtcgaatgtc gaatacttcg
     6961 aacgcgtccc acgaatacca cgctcccaat tgaaaaagat gcgtgaaaat ctattgatcg
     7021 gctcagcttg tgataaggga gaaatattcg aagccatgat gcaaaaaggg gtagaagagg
     7081 caagaaaccg agcaaaattc tatgattata ttgaagttat gccaaaagcg gtctatgcgc
     7141 ctttgatcga gcaagaattg gtaaaaaatg aacatgattt ggaagaaatt atccaaaatt
     7201 tggtagaaat cggaaaaagt ttggataaaa tcgttgttgc aacaggtaat gtacattatc
     7261 tgaacgaaga agatgccatt taccgaaaaa ttttgatcaa ttctatgggc ggagcgaatc
     7321 cgttgaatcg tcatagtttg ccggatgtcc atttccgaac aaccgatgag atgctaactg
     7381 catttcactt tttaggggaa gaaacagcga aagagatcgt agtagaaaat acgaataaaa
     7441 tcgcagatat ctgcgaagaa gtgattccag tcaaagatga actttataca ccgaagattc
     7501 caggttcaga agatgaaata tcagaattga gttacacaaa agcaaaacaa atgtacggag
     7561 atccgttacc tgagatcatt caaaaacgat tagaaaaaga gttgaattct attaatggta
     7621 atggcttctc ggttatttat ctgatcgccc aaaaactagt gcataaaagt aatgaagatg
     7681 gctatctagt aggttcacgt ggctctgttg gctctagctt tgttgcgacg atgacaggaa
     7741 tcactgaagt taatccatta gcccctcatt attattgtcc agagtgccaa tattccgagt
     7801 tttttgaaga tggtacgtat ggttctggat ttgacatgcc agaaaaacaa tgtccaaaat
     7861 gtggtgcacg cttaaacaaa gacggtcatg atattccatt tgagacattt ttaggattcc
     7921 atggagacaa agtgcccgat attgatttga acttctcagg tgattatcaa gcagaagccc
     7981 ataactatac gaaagtattg tttggcgaag actatgttta ccgtgccgga acgattggta
     8041 cagtcgccga caaaacagca tatggctatg taaaaggata cgaaagggac aataatctgc
     8101 agtttcgaag tgcagaagtc gacagattag caaaaggcgc tactggagtc aaacggacaa
     8161 ccggccagca tccagggggg atcatcgtta ttcccgatta tatggatgtc tatgatttta
     8221 cgccgatcca atatccagca gacgatcaaa attcagaatg gaaaacgact cattttgatt
     8281 tccactcgat tcacgataat gtattgaaat tagatattct agggcatgat gacccaactg
     8341 ttattcggat gcttcaagat ttatcaggga tcgacccaca aacgatccct acggatgacc
     8401 cggaagtgat gcggatattt gccggtcctg aagtacttgg agtaagtcag gaacaaattt
     8461 attccaaaac agggacatta ggtattccag aattcggcac tcgttttgtt cgagggatgt
     8521 tagaagaaac gcatcctaca acttttgcag aactactgca aatttccgga ctatctcatg
     8581 gtacagacgt atggttagga aatgcggaag aattgattcg acgaggagat gcgacactgg
     8641 cggaagtaat cggctgtcgt gatgatatta tggtttattt gatccatgct ggattagata
     8701 gcgggatggc atttaaaatc atggaaactg ttcgtaaagg acaatggaac aagatccctg
     8761 atgaattgcg agaaacctat ctatcggcga tgaaagaaaa taatgtgccc gactggtata
     8821 tcgattcttg ttcaaaaatc aaatatatgt ttccgaaagc ccatgctgca gcttacgtat
     8881 tgatggctct tcgtgtcgct tatttcaagg tttatttccc tattttgtat tactgtgcat
     8941 acttttctgt acgtgcagac gactttgatc tagtctccat gtgcaaagga aaagatgctg
     9001 tgaaacaagc aatgaaagaa atcacggaca agggattaga tgcttccgta aaagaaaaga
     9061 atcaactgac agtgctggag ttagccaatg aaatgctgga acgaggattc aaatttggaa
     9121 tgattgattt gtataaatcg gatgccgtta actttgttat tgaaggtgat acgttgatcg
     9181 caccatttag agctgtccca agtttgggaa ccaacgtggc gaaacaaatc gttgaagcaa
     9241 gaaaagacgg tccattttta tccaaagaag atcttgccac ccgcggtaaa gtttcaaaaa
     9301 cattgatcga atatatgaac gacaatggcg tattgaaaga tctaccagat gaaaatcaat
     9361 tatcgttatt cgatatgcta taagcatctg ttttcctgtc tgcttgtaat tttaagcaga
     9421 gtatggtata gttcttatag gtaatgatac taactgtgag tgagcggatt tttcgctcac
     9481 tctttttaat ggaattacgc acttaatttt taaggaggcg aaacttttga gtagcgtcgt
     9541 tgaaacagtc acagaaatgg tgacaccgat tttagatgaa caaaattttg aacttgtcga
     9601 agtcgaattt gtaaaagaag gaaaaagctg gttcttacga gtatttattg acaaagaagg
     9661 cggtatcgat atcgaagaat gcgcctttgt cagtgagaaa ctgagtgaaa aacttgatgc
     9721 agcagatcca gatccgattc ctcaagctta tttcctggag gtatcctccc cgggagcaga
     9781 gcggccattg aaaaaagaaa gcgactatga aaaggcactt ggtgattatg tccatgtctc
     9841 cttataccaa ccagtagatg gcgaaaaaca atatgaagga tttcttcaat catttgatgc
     9901 tgaacagctt acattgaaaa tacgcataaa aacacgagaa aaagaattga cttttgaccg
     9961 aaaaaatatt gcaaaagctc gcttagctat ccaattttaa tcacggagag gaatcagaaa
    10021 aaaatgagta aagaaatgtt aaatgcgtta gacgctttag aagctgaaaa agggatctca
    10081 aaagaaatcg tgatcgatgc attagaagca gcattggttt ctgcatacaa acgtcactat
    10141 ggccaagcac aaaacgtcga agtggaattt gatcagaaaa aaggaaagat ccatgtctat
    10201 gcggtaaaag aagtaacgga agaagtgatg gattcacagt tggaagtctc attgaaagat
    10261 gcacttttaa tcaatccagc atatgagatc ggtgatacga tccgttttga agtaacacca
    10321 aaagattttg gacggatcgc tgcgcaaacg gctaaacaag ttattttaca gcgtgtacgt
    10381 gaagcagaaa gaacgattat ttataacgaa ttcagtgctt atgaaaaaga catcatgcaa
    10441 ggaatcgtcg aacgccagga taaacgttat atttatgtca atcttggtaa aattgaggca
    10501 gttctttcta aacaagatca aatgccaaat gaattttatc aaccacatga tcgaatcaaa
    10561 gtttatgttt cccgagtaga aaatacgtct aaaggtccgc aagtatttgt tagccgcagc
    10621 catccagacc tattgcgtcg cttatttgaa caagaagtac cagaagtcta tgatggaatc
    10681 gttgaaattg tcagcatcgc ccgtgaagct ggcgatcgtt caaaagtggc agttcgctca
    10741 aatgatccaa atatcgatgc agtcggtaca tgtgtcgggc caaagggaca acgagttcaa
    10801 gcaatcgtca atgaattaaa aggcgaaaac atggatatcg tcgaatggaa tgaagatcca
    10861 gctgtattca tcgcaaatgc attgaatcct gcacaagtca tggatgtgat cttcgatgag
    10921 acaaattcaa aggcctgcac agttgtcgtt ccagactacc aattatcgct agcaatcggt
    10981 aaacgcggac aaaatgcacg tttagctgct aaattgacta accataaaat cgatatcaaa
    11041 tcagaatctg acatgacgga attttatgaa aatcaagaac aacaaaaaga aacagaagaa
    11101 ctgcatgatg aagcaatcgt tcagtcagat ttaacagacg atgaatatga aacaattgca
    11161 ttcaacaatg aaacggtcga agaaaaacca gaagcttaac tttagtaagg aggcaaagag
    11221 atgaaacaaa ggaaaattcc tttacgcaaa tccgtggttt caggtgaaat gaaacctaaa
    11281 aaagagatgg tccgaatcac ccgttcaaaa gaaggggaag tatcgatcga tccaactgga
    11341 aaaatgcctg gacgaggagc gtatgtctca cttgagccgg aagaagtaca acaagcttgg
    11401 gataaaaaaa ttctcgatcg agtactcgaa acaacattaa cagatgagtt ttatcaagaa
    11461 cttcttgact atgtcactca ccaaaaagca cgtaaagagt tatttggaaa atgaatacag
    11521 ttaatcggca aaaagccatg aatctcatcg gtctagcgat gcgcgcggga aaactaatca
    11581 cgggtgaaga actgaccatt gctgaaatcc gcaaacaaaa agcaaaattg gtgtttgtgg
    11641 caaccgatgc aagtgagaat acaagaaaaa agataaaaga taagagttca tattatgaag
    11701 ttccttgctt tgagctattt tctgaagcag aaatcacgca gatgatcgga aaacctcgca
    11761 aggtattcgg aataatggat gccggatttg cgaagaagac caaggagcta attgaaggtt
    11821 aggaaggtga ttgcatgggc aacaaaagaa tctatgaatt agcaaaagaa ctcaaaaaat
    11881 ctagtaaaga cgtcgtggaa aaagcacaac aacttggcat cgatgtgaaa aaccatatgg
    11941 gtgcgattac tagcagtgat gagaagaaat tgcaacaagc gttcaaaaac ccaagcacga
    12001 aacaacaagc tgcacaacaa gcaagcagaa aaccagccaa tcaacagccg aacgatcaac
    12061 gtaaaaaaga aacaacgaac aatcaacaaa aaaataaaaa taaccgcagt tatcaagatc
    12121 gcggtcaagg gagcggtcaa gtgaatcaag gaaaaaaacc aacaacaaac caaaataata
    12181 cgggaaatac cagcagccaa aatcgtcaag gaacgagcca aggcggcaat caaaaccgta
    12241 ctaacaataa caacaaacca aacaataaca atcgcggcaa atttaataat aataaccgta
    12301 atcgttataa taaaaaaggt aaaaaaggca aacagcaaca atcaactaaa ccagctgtac
    12361 caccacgcaa attccgtgaa ttgccagatg tattagagta tactgaaggt atgaacgtag
    12421 cagatattgc gaaaaaaatt catcgtgagc cagcagaaat cattaaaaaa ttatttatgc
    12481 ttggtgtcat ggtcaatcaa aaccaagcac tggataaaga tacgattgaa ctgttagcga
    12541 cggactatgg catggaacca caagaaaaag tccaagttga cattgctgat atcgataaat
    12601 tcttcgaggc agaagaagtc gatccagata aattagtctc acgtccgcca gtagtaacga
    12661 ttatgggaca cgtagaccac gggaaaacaa ccttattgga tacattgcgc cattcacgtg
    12721 taacaagcgg cgaagccggc ggaatcacac agcatatcgg tgcctaccag atcgatattg
    12781 atggtaaacc aatcactttc ttggatacac caggacatgc ggcctttaca agtatgcgtg
    12841 cacgtggagc aagtatcaca gatatcacga tcttagttgt tgctgcagat gatggcgtca
    12901 tgccacaaac agttgaagca atcaaccatg ccaaagctgc aggcgttcca atcatcgtcg
    12961 ctgtaaataa aattgataaa ccaggtgcaa atcctcagca tgttatgcaa gaattaagtg
    13021 agtatgaatt aattcctgaa gcatggggtg gagaaacgat ttttgttgag atctcagcga
    13081 aattcggcca aaacatcgaa gaattgctgg aaatgatcct gttagtcgca gaagtagaag
    13141 atttaaaagc agatccaaca caacgcgcaa tcggaacagt gatcgaagca cgtcttgata
    13201 aaggtaaagg tcctgtgtca accttacttg tccaagaagg aacacttcac gttggtgacc
    13261 caatcgttgt cggtaatact tacggacgtg tccgtgtaat gacaaacgat cttggccgtc
    13321 gcgataaaga agcaggacct gcaacgccag tagaaatcac tggcttgaat gatgtacctc
    13381 aagcaggtga ccgtttcgta gtcttcgaag acgaaaagac agcacgtgca gcgggtgaag
    13441 aacgcgcaaa acgtgcatta ttggaaaacc gtgctgccac aagccgtgtg actttggaca
    13501 acttatttga aagcctgaaa gaaggcgaat tgaaagaagt taacgtaatc atcaaagcag
    13561 atgtacaagg ttcagcagaa gcactggctg catcattgaa gaagattgat gtggaaggtg
    13621 tccgtgtaaa aatcgtccat tcagctgtcg gagcaatcaa tgaaagtgat gtaaccttag
    13681 cagcagccag caacgctatt atcatcggtt tcaacgttcg tcctacaccg caggcaaaag
    13741 tgcaagctga tactgaagaa gtcgatattc gtcttcaccg tatcatctat aaagccatcg
    13801 aagaaatcga aactgcgatg aaagggatgc tagaccctga atttgaagaa aaaatcaccg
    13861 gtcaaatgat cgttcgtgaa accttcaaag tttctaaagt tggtacgatt gcaggtgcct
    13921 atgtaacaga aggatatatt cgtagagata gcggtgtccg tgtaatccgt gacggaatcg
    13981 taatctatga aggccaactt gcaagtctga aacgattcaa agatgacgtg aaagaagtca
    14041 aaatgggtta tgaatgtggt gcgatgattg aaaaattcaa tgacatcaaa gtagatgacg
    14101 taattgaagg atttatcatg gaagaagtca aaaatgacta aaaactgata aaaggaggcc
    14161 aatcattatg gctaactacc gtgatcgccg agtcggtcaa gaaatcttaa aagaagtcaa
    14221 tgatattttg cgcaaaaagg tccgtgaccc tcgtgtggag aacgtaacga tcacagatgt
    14281 gaaagttacg ggggacttac agcaagcaac gatctattac agcattttat ctgacttagc
    14341 atcagataag aaaaaagcac aagatggatt gaataaagca tcaggattga ttcgtcgaga
    14401 gttagggcac aatctaagta tttataagac acctgaattg ttctttgaac ttgatgaatc
    14461 agttgtctat gggaaccata tcgatgaatt gcttcgcgac ttgaacaaag actaatcaaa
    14521 aaatcccgca aatcctgtga aatcaggggt tgcgggattt ttttgttttc attaagacgt
    14581 attacctaag agttccatga ctttagtctg gtagcgtgtc agctaaccaa aaaagagaaa
    14641 aagaaaagta aaaaattcga ctttcttttt tctatagtat ataaattttt gcatatagat
    14701 aaagatcaag ataccgataa cggaagctag attgtaatct attttagtga cagtcaggta
    14761 tagccaagta actagaagat cagtggaacc tgcaccataa aagtttgagt ttgtcggtcc
    14821 gttgacagtt aaaagaaaaa tcacgttgaa gttattgata tttccgatta cgccgcttcg
    14881 atttggtcac gatccaaatt ttggataatc cctgtagaaa ttagcatagt agctgggata
    14941 ccaattcaca tattgacaaa gataacagag acttttacat aaaacggatc aatcaggaaa
    15001 ggaattgctc gatccagccc cagttttcca acaatgtgtt aactggccta gcgtcattca
    15061 gcaaattatg catgatgagg agcgaaatga actgcggaac agccattgtg ataacgacaa
    15121 gttttttgga tcaatgtaaa aaacgtcagc gaaaggaaaa atttcgtggt tttttatacc
    15181 tgttcatttg gctagatata aaatccaaac aaatgaacag aagtgcttat taggtttcaa
    15241 ttgggagtat ctgcgctaga ataagagtaa taatgaaaag gagtgttatg tatggattat
    15301 tctagtaaag aatactttga taaaatgacg gcttggtggc gtgctgctaa ttatctttct
    15361 gttgggcaac tttatcttaa agataacccc ttactgagac gtacactgaa accagaagat
    15421 gtcaaaaaac atccaatcgg tcattgggga acgattcctg gccaaaattt tatttatgtc
    15481 catttgaacc gagtcatcaa taaatatgat ttgaacatgt tttatatcga agggccagga
    15541 cacggcggac aagtcatggt atcgaatgct tatttagatg gaagttacac agagatctat
    15601 ccagaagtaa cggaagatga gacaggaatg caaaaattat tcaagcgatt ttcatttcct
    15661 ggaggcattg cttctcatgc agcaccagaa acaccaggtt ctatccatga aggcggagag
    15721 cttggatact ctctttcaca tgcagtcgga gctgtattag acaatcctga agtaatctct
    15781 gctgtcgtga ttggtgatgg tgaagcagaa accggtcctt tagctggaag ctggttctca
    15841 aatgtcttta tcaatccagt gatagatgga gctgttttac cgatccttca tttaaatggt
    15901 gcaaaaatag ccaacccaac gatcttggca cgtaaatcag atggagaatt agcaaattac
    15961 tttaacggac ttggctggga gcctttcttt attgaaggaa atgatccaga aaaattaaat
    16021 cctgttatgg ctgaaaaaat ggatcaagca atcgagaaaa tcaaatccat ccaaaaagaa
    16081 gctcgcctga agacagcaac agatgtcgtg atgccaaaat ggccagtatt gatcgtacgc
    16141 acaccaaaag gatggaccgg tccaaaagaa tgggacgggg aaccaattga aggaaccttc
    16201 cgagcacacc aagtaccgat tcctgtagat caagaacata tggatcatgc agatgcttta
    16261 ctccgttggc tgaaatccta tgaaccagaa aaattatttg atgcacaagg acgtatttta
    16321 gaagaaatca gagaaattgc gcctactggt gaccaaagaa tggcaaaaaa tcctatcacc
    16381 aatggtggaa tcgatccaag accgcttatt atgccagatt ggaaaaaata tactttgcaa
    16441 tttgaaaaac taggctccgt taaagcagaa gatatgaccg aacttggtaa attcgttcgc
    16501 gagatcattg agaaaaatcc agaaaatttc cgtatatttg gaccagatga aacaaaatca
    16561 aaccgattga atcaagtttt caaaacgaca aatcgccaat ggatggaaaa aatcgaacca
    16621 gaaaatgatg aatggctttc gccatctggt cgcgtcatcg acagtcaatt atcggaacac
    16681 caagatgaag ggttcttaga agggtacgtt ctgactggac ggcatggttt ttttgcaagt
    16741 tacgaatcat tcttgcgagt ggttgactct atgttgactc agcacttcaa atggatacgg
    16801 aaatcacatg acctttcttg gcgaaatgat tatccttcgc taaatctcat tgcaagctca
    16861 acagtcttcc agcaagatca caatggatat agccaccaag atccaggaat attgacacat
    16921 ttggcagaaa agaaggcgga gttcatccgt gaatatctgc ctgcggatgc aaatacattg
    16981 ctcgcagtca tggataaagc attccactcg tcagaaaaaa tcaatttgat catctcttcc
    17041 aaacatccgc gtgctcaatt ttactcagca gaagaagcag ccgtcttagt gaatgaaggc
    17101 ttgaaaatca ttgattgggc ttcgacagca aaagaggagg aacctgaatt ggtcattgct
    17161 gctgcaggaa cggaatctaa cttagaagca ttagcagctg taactttgct gctggaagaa
    17221 ttcccaaaac taaaaattcg gttcatcaat gtcgtagatt tattgaaatt acgtcatcca
    17281 agtcaagatc cgcgtgggtt gagcgatgaa gaatttgatc aatattttac aaaagacaaa
    17341 ccgatcttat ttgctttcca cggttatgaa acattggtcc gcacgatttt ctttgatcgt
    17401 cataaccatc atttaatgat tcatggctac aaagaaaatg gagatatcac gacaccattt
    17461 gacatgcgtg tagtaaatga acttgatcgt tatcatttgg caaaagacgc agctttaaaa
    17521 atcaaaggaa gtcaagcaga agattttgcg aaaaaaatgg atcagaaatt acaagaacat
    17581 caaaactata ttcgtgaaaa tggtattgat ctgccagaag tattggattg gaaatggaaa
    17641 aatcttgacc aataaaatga taaaatcgat cccgcaaaat agaatttttt acattgatta
    17701 agaaactgaa aaaataaata aaaaaaacgg aaaaaagact ggaagaatgg ccagtctttt
    17761 ttttcgtttt tgggaagtga aaaccaaact atttacctgt aagccaccca taaatccaat
    17821 caaatggtgc aaagaaaaaa tcttccattc tattccctcc tttaaaaaag cataacatga
    17881 tttctattta cgtgagcatg aattttgtta cgtttttatt gccgaaaaat aaaaaccgtt
    17941 cattatgatg tcaaattcgt cagtaattga tgcaatgtgt tataataatt cagttaaatc
    18001 aaaaatgaaa tggagaaaag acatggacgg gttattacct ttatggaaag aacgcggaat
    18061 gacaagtcat gattgcgtat tcaaattaag aaaaatctta catacgaaaa aaatcggaca
    18121 tgggggtacg cttgacccag acgtagacgg tgtgttgccc atttgtattg gaaaagccac
    18181 aaaagttatc gaatatttga cagattctgg gaaaatctat gtaggcgaga taactctagg
    18241 tttcagtaca acgacggaag atcgatccgg agaacgggta gaaacaaaac cagtagatat
    18301 gtctttatct gaaaaaaaaa tagatgaagt gatggcttca tttattggtg agatcaccca
    18361 gataccacca atgtactcag cggtaaaagt caacggtaag cggttatatg aatatgcccg
    18421 caataatgaa acagtggagc gtcctgttag aaaggctcag atttaccgtt ttatccggac
    18481 aagtgaattg acaagagacg aagtgacagg aaccatttct tggcgcttcg aagtagaatg
    18541 cgggaaagga acctatgtcc gcacattagc tgtagataca ggcaagaaac ttggttaccc
    18601 tgcgcacatg tctgatctga cccgaacagc aagtgccggt atgtcccaga aggatgctct
    18661 gacattagaa gaagtagctc aagctgtgaa aaatgggaca atcgatgatt gtttgctgcc
    18721 aatagaaaaa ggagtggaac ggttcaaacg agtagatatt actgatgaag tatggcagaa
    18781 agtaaaaaac ggtatgagac ttgattatac tgtatttgcg ctcccagaaa tgccgtcaga
    18841 agaaattgct ttgttttata aaggaaaagt tgtcagtatt tatcagccaa acccgaaaga
    18901 aaaaaacaaa ttaaaaccta gtaaagtatt gagaaatgag gaataacgaa tgaatatcat
    18961 caagatcaga catccttacc aatcagaaca aattccagaa gacgatgtcg ttttagtgct
    19021 gggatttttc gacggcgtgc atatcggaca ccagaaagtc ataaagacag gtatggaaat
    19081 tgcacaaaaa gaaggattga aattagcatt gatgactttc aatcagcatc catctattgt
    19141 ctttaaaaag atcgatccta gcagcgtgaa atatttgact agccttaagc aaaaagaaga
    19201 aaaaatggcc gctttgggta tagattattt atatgaagtt gattttactt cttcttttgc
    19261 tcatttagca ccgcaggaat ttgttgatca atacatcgta gggttacatg caaaatatgc
    19321 tgtttccggt tttgattata cgtatggacc aaaagacatt gcagatgtcg ctcattttcc
    19381 tgaatatgcc aaaggaagat tcaaaatcgt cacagtttct aaagaagaag aacaaggtga
    19441 aaaaatcagc tctacccgta ttcgtggatt actagacagc ggacaagtaa aagaagcagc
    19501 aaaactatta ggttgcacat atgaggtaga aggggtagtt gtccatggcg atgcacgagg
    19561 cagactacta ggattcccta cagcaaatgt caaagtaaag agcacggttc atttgcctaa
    19621 agaaggtgtt tatgtgtcgg aaataaaaat tggggaaaca tggtatccag ccatgggttc
    19681 aattggacat aatgatacat tcggtgaagg acgggagctg actgtagagt tgtatatttt
    19741 ggactttaat caagatatct atggtgaaca tgtcgcggta cgctggaatc aatttttaag
    19801 agatcaagtg aaattttcta gtgtagagga attgattgaa caactcaagg aggatgaaca
    19861 agcaacagct gaatattttt ctcagcaata aaacattatg cataaaatct cttgtttatg
    19921 tataactagc aattacaata aaatgggtga taaataagcc caggaaaggg gctgtgacaa
    19981 aagtcttgtc acagcctttt tttccaaata aatggttttt cagaagtagc ttcttcggaa
    20041 ataagccgaa atttcttgaa gctgaccact tctgtcacaa cctctttctt tatagaaata
    20101 aaaggacact agcaattcat gaaaacgatt gctagagcgt atcatttgct ttagactata
    20161 tatcttggtg tacactcact ttaaggcgga aataaaagtt atccgtaata acaggtttag
    20221 gtatgaaaaa ggagtgggta ctatgtacaa ggaattagaa aacaaagtcg cagtagtaac
    20281 tggtggctca aaggggattg gaacagcgat tgctcaacga ttcgggaaag aaaaaatgaa
    20341 tgtagttgtc aactataact cggataaaga aggagcagaa aaagcagtca aagcaatcga
    20401 agaggcaggc ggaaaagcag tctctgtaca agcagatgtc agttctgaag aaggaataca
    20461 gcgtttactt gatacggctt tagaaaattt tgggacactg gacgtttggg ttaataatgc
    20521 tgggatggaa aatcagcatc caacccatga acttagcctt gaagattggg aaagagtgtt
    20581 aaaagtcaat ttgactggcg tttttatcgg aacgaaagcg gctttaaatt actttacaga
    20641 gaataataaa aaaggaaata tcatcaatat gtcttctgtt catgaacaga tcccatggcc
    20701 aacgtttgct cattacgctg cttcaaaagg cggggtaaaa ttatttaccg aaacggtagc
    20761 aatggagtat gcaccgaagg gaatcagaat caatagtata ggccctggtg ctatccgtac
    20821 acctattaat gcggcaaaat ttgatgatcc tgaacaaaaa gaactcttgg aaagtatgat
    20881 cccactagga cgcgtaggtg aaccaaaaga aatagcggcg acagctgcat ggttggcttc
    20941 agacgaatcc agttatgtta caggtattac actatttgca gatggtggga tgactttata
    21001 tccttctttc caaggtggaa aaggataagg gtgaataaaa atgggtttat tgattgcatt
    21061 gatcccggca gttggctggg ggatccagcc actgattttg aaaaagattg gtggcagacc
    21121 aacaaatgag atattaggaa ctggtattgg agcggtgctt gtcggcttac tcgttcaatt
    21181 attcgcttct ccagctggta tttcagtgac tacatttttg attagtttac tttctggtgc
    21241 attttgggtt attggacaaa caggacagta tacatctttc aacttgattg gtgtttctaa
    21301 aacgatgcca atttcgactg cattacagtt agtcggaacc tcgttgattg gtgtcttcgc
    21361 ttttggagaa tggtctggaa gtatggggaa aatcattgga gcagcagcaa tcattcttct
    21421 tgtatttggt tctgctttaa cggcaatcag tgatgatcct ggaaaaaaag gcggtatcac
    21481 aaaaggaatt agtctattag catcaacaag tattggttat tgggtataca gtgcattgcc
    21541 taaactagtt gatgcaagcg gactttccat tttctttccg caaatgatcg gtgtcttttt
    21601 aggggcagtg atttatgtga ttttccgtaa accaaccgca ttttctgaag acaagagttg
    21661 gaaagcaacg atcgttggag ccatctttag tgtgtcagca ttggcttata ttttttcagc
    21721 aaatgaaaat ggggttgcta ctgcatatat cattacacag ctaaacgtag ttatctctac
    21781 attaggcggt atgtacatcc tgcatgaaaa taagacacca aaagaaaaaa aattaacgat
    21841 tatcggatta ttgctgatcg tagtaggaag catggtcacg gtctttattt gaggataaag
    21901 atggtaaaat ttcatttatt aaaaaaatat ttgtttgaac caaacagtaa gtacttataa
    21961 aaaaatagcg ctagaatttc caacatctct ggaatttcta gcgcttttta attgtttaag
    22021 atacaaggga aatatctgtc tggatcaata atttttcatt gatccattga ctaaagttta
    22081 cgacataatt gaagttacca ccgattagta tctccgcatg attgtcttta taaagatcgt
    22141 attgtaaaag acggtctttt ttatttgcgt aaacatcgtt atggtactgg actaattgtt
    22201 ctcggaattg attgattgct gtaaaatcac gatattgaag gttgaaacgc atcgtgattg
    22261 gtgcatttag catgaatcgc tgcatttctt ttgctgcttc ctctgcttga tctccgtata
    22321 ggaactcaaa aagattacgt tcagaaaatt tccattgccc gccaaccttt gttcctttta
    22381 gttttccttc atgaagataa tttctgatag aacgtgtagt aacacctaat tgttcggcta
    22441 attcatttac tgagtaaaaa tctttcaatg ttcaccctcc tatgtgaatg gttctactga
    22501 cttagttgat atttgcttaa tcacgaaatg tagaaagaac gattttaatt aaggtcattg
    22561 taaaaatcaa gtcagaaaat tctaactttt atttctctaa agaaattcgt tctctcctaa
    22621 agtgatatta tatttaaata tacctgaaaa taatgggcag tattatgaaa gatcactgat
    22681 attttctcgt attttttcct tattcaaaag ttttgcagga aaattgctga aaaaataatt
    22741 ttactaagta gaaaattcaa agccattcaa tcagtgattt ttttcagcac aagaaagact
    22801 ccttataaaa atagcagata ctttgtcaat taagtgttta accagggatg ataaaaagga
    22861 taggattgaa caaaacctgt ataaggtgat gaatagtttc ctcgccagta aaaggtttat
    22921 tcgtataaag ctttatcgcc aaaatttatc atgacctttt ttatcttcaa tattaaatga
    22981 aatcaattgt tcaatcaatt cccagttgat tacacttttc caaggaactt tgatgacgtt
    23041 atccgtatat acgtagcctg cttcttggat atttttttca agggcacgaa tcgctgcttt
    23101 ttccggtgcg atagaaaaat gatttttagc aaaactaaat gcaataataa aggtaccatg
    23161 gtcagtaaac ataggttggt tccattttac agttgtgtct agctggggat acgtttctgt
    23221 tatcttagca aaaaggtctt tgatcttttg ttgatgttct ttttctttga tttgattgat
    23281 gatcggctga aatttttcca ttctaaaacg cctccattaa gtggtttgat ttattttacc
    23341 agaaaaacaa tagttatgta taaaaaacga acaaaacaca agtaatgttg taaccgctat
    23401 atttatatga taagatgtct caaataataa aatagaaaag agacataaaa atgattgact
    23461 tggcaaagaa attcttacac gaaaatatat attttgagtt gtatcatcat cgagccattt
    23521 atacaaatga agatgctttg gtaataaaga aagaacaagg atttacaggt acagagacca
    23581 aaagcttatt cttaaaagat aaaaaagata ataactacgt atatctgact tttactacga
    23641 aacaaacgga tttcaaagca ttagggaaga tcgtcggtaa aaggctttct attgtttcat
    23701 cagaacgcat ggaagaacaa acgggacaaa agcagggcgc tgtttctccg tttggttatg
    23761 aaaaacagat tccgattatt attgatgaag agctcttatc aaatgaaaaa cttgtttttg
    23821 caccaggtag accagatcaa acgatggtca tccaaacggt tgacttaaac aaaatcatcc
    23881 agctacttga gatgccgata tttttgatgc cggcaaacga aaaaaacaat taaccaagta
    23941 aaatagtgca tacttggagg tgaagcaaca tgataagtta tccattatta gaaagaaatg
    24001 caacgattgg ggttactgca ccatcctcag gcgtatcaga ggaacaccat tcgttactcg
    24061 aatcagcttt aaagagaatg gaagaaaaat ctttccaagt agttactgga aagacgcttt
    24121 ggacgcaaga taaggaaaaa tcaggtccag caagaaaacg agcagatgaa ttcaatcaaa
    24181 tgatgcaaga tgaaaatatc caaatgatct ttccgacttg gggaggagaa ttattgattg
    24241 agattctcga ttttgtcgat tttgatgctc tacaaccaaa atggatagta ggttattcag
    24301 atattagtac gttgctactt gctgtcacat taaaaacagg gatagccact gcacatggaa
    24361 ctaaccttat tgatttaaga ggagagtact tggatgaaac gacagcaatg tgggaaagtg
    24421 tactgaaaac aagagaagga gaatcgattc tacagcaatc ttctgaaaag tatcaaaaag
    24481 tgtggcccca tgagcattca tcatcttctg ttttccatct cactgaacca acaaattggc
    24541 aatcagtcag cggagatgca gtaaggatga agggacgatt attaggtggt tgtattgata
    24601 cgatcaggca tttaatcggt actccttatg gagatgtcca atcttttaaa agaaagtata
    24661 atgatggaaa gccgatcttg tggtttctag aaaactgtga gctatcgtca gttgatttaa
    24721 gaagatcact tgttcagatg aaactggcag gctggtttga tggcagctcg gggatcatgt
    24781 tcggtagaag tgcggcagag gatttgttag attataaaat gaaggatgta tacgaagatc
    24841 tgtcaaagga attaaaacta ccgatcttgt atgatattga ttgtggtcat gtaccgccac
    24901 aactgacatt gattaatggt gcctatgcag aagtagcggt agaagataga aaagcgactg
    24961 ttttgcaaac gtttatttaa aaaatttttt cctgccttgg agtctatcaa agaccaataa
    25021 atctaaatag aaaagaaagt gaaagaagct gacaaaatgc tgtcagcttc tttttgtata
    25081 acatattaga aagtctaatt atctataact gtcacatcga ttaactgttt cacgtcattg
    25141 ggatttcctg taggaatagc tactacagat gcattatcaa tgatttcttg agttgtttct
    25201 ctaatgaacg ctggataaga tgtttgatct gcaggcattt gccaatgtga aggaactgga
    25261 taactagtga ttgtcccgtc atggttactt gaatacacta aaactccctg gtaactgtat
    25321 ttcccagtga gcataaccac atctttcgga tagactgcac tccctgcatc ataaggggcg
    25381 ataggtgttc ctgcaggata atgaataaca tgtaattcga aatcacccga tgtatctcta
    25441 aaagtattac ctctaatagc tagccaaaca cgtgcgtact caatttgtag atcagaatat
    25501 cctgctaacg gatctattga ctgagaagac tgtgaggagg ttgtgtctat tgttgaagaa
    25561 gaaatatttg tctcaactgt actactagat gtagaagaag tattgggctc tgagacttta
    25621 ctgcttgttg aagatgtatt cgtttcagta gaagctgatt tctgagtact tgatttttca
    25681 gctgaactgg tttgtctgat tgaacttttg tctgtggtcg cagatggatc actattttct
    25741 ttactaggag acgtacatcc tacaaggaat aaagagacca acaataaaat aaaacctaac
    25801 tttttcatat aaaatacctc attatttttt taataccagt atagcataat cctgaatttt
    25861 tgaatccgta aaaccttgtc tatgaatata aatagagtgt atcttctttg ataaatgtca
    25921 gaaaaagtgt gacaaataat catcagtaaa attacttgtc acactattga taaggagtat
    25981 ttaattgaag agaatgatta ggagaaagga tctccgtact ttttatagta atcatagaat
    26041 ccaagtttgt atagacgaat cgttttttct cttatttctt ctgtttcaaa atggactgct
    26101 tgaagaatca agtctgtgaa ttctatagga gctgttccat tcgcagtaat caaattacga
    26161 tctttcacag cttgttgttc tataaaatct tctttattct gataattttc cgattctaac
    26221 caaggaaatt gcgcatttcc ggtgtgtttg taaccttgca ataatccgct ctgagctaaa
    26281 taatcaactg ccccacaaat agcagctact ggtacgtctg tttttaacaa tttatcgatt
    26341 atttgatata aatgatgatt ttttatgtcc catgaattgc caccgatcat aataaaaagg
    26401 tcaacttttt caggtatttg ttcgattaag taatcgattt ttgtttttaa tcctccaatg
    26461 gaagtgacct ctgattggat agaagcagtt ttgttttgcc atttctcatt ttggtttagt
    26521 gtgctagtaa tataagcacc ttcccaatcg gcatattcct ctaatagaaa cgtaatcgca
    26581 gttttcattt tcaagctctc ctttactatc tataacaagg acaacagttt taacctacag
    26641 acattcatta aaattgggta gcgacagcga aaacaacaat tcttaagttt tagacagagt
    26701 atacagatag tcaacttaat taaatacgaa ctaaataaaa atgacaggta atcatttttg
    26761 tttaccagca ttataacatt ttgcatacat ggatgacaaa agaaccaatt cccacttgct
    26821 tgagtacaat attaaaagaa actggaaggc aaaaagtaat tatagaaacg aaaaataaat
    26881 catatcgggc actcagacat gaaaacaacc ctagagatat ataactaggt aaccagttta
    26941 atgaaagaaa aaatcatcca agaagtcgat tcttggattt tttagtggat ttgttcgcca
    27001 aatacgttta gtatgcgtat atgagaggtt gtaagcaaag cgttgtggtc tgagcactta
    27061 aggaggaatc tgtgaaaata gcttttcata tttttgcaga ttttgactta atgccgaagg
    27121 tcagctttgc gattaccgtt tatcgagagg ctgtaaaagc accgtgtagc tccgatgaca
    27181 ataagcaagc atttttagag ctaggtttcc ttgattctaa atatgcggct tatttcaatg
    27241 agttggtgct tgaacgcgat tttaagaagg tcgaaaacag gcacgacttt tcgacgtaag
    27301 gagaaagaat ttcaatgata gaaacaataa atcccgcaga aaacaaggtt tctgcgtaca
    27361 ttcatatccc attttgcgag catatctgct attactgtga ttttaataaa gtttttttag
    27421 aagggcagcc agtagatgag tatgtggaaa tgctgctgaa tgaaatgaaa ataatgctag
    27481 aaagtcatcc gacagcgaat ttagaaacac tttacgtagg gggcgggaca ccaacttctt
    27541 tatctgctaa acaacttgat cgactgttat caggtgctag agagatcctg ccttttcaaa
    27601 atggtaatga gtttactgta gaagcgaatc caggtgattt gacccgtgaa aaacttcaag
    27661 taatgaaaaa ctatggcgtc aatcgtttat ccatgggagt gcagacattt gataatcgct
    27721 tattgaaaaa aattggacgt aaacacaccg cagaagatgt gtatcaaact atgcaatttt
    27781 tagaagcaga aaattttacc aatgtaagca tcgatttgat ttatgcatta ccgggacaaa
    27841 cactagaagg atatctggat actttggaaa aagctttggc tttggatctg cctcactatt
    27901 ctttgtactc attgatcctc gaaaacaaaa caatgttcat gaactgggta cgacaaggaa
    27961 gactcgaact gcctgatcag gaaacggaaa ccagaatgtt tgaagagacg attgaagcaa
    28021 tggaaaagca tggacgtcac caatatgaaa taagtaactt tgggctctcc ggacatgaat
    28081 ctcaacataa cctcatgtac tggaacaatg accactattt tgggtttgga gctggtgcta
    28141 gcggttatct tggagatatt cgttaccgca acaaaggacc aatccaacac tatttacgcc
    28201 cattacgagc aaatgaacgc ccggtattgg aagaagaaaa attgacacga aaaaatcaaa
    28261 tagaagaaga aatgttctta ggactaagaa aaaaaacagg gattactaaa aaacatttct
    28321 atgatcgcta tcaaaaaaca attgaatcgg tgtacggtga tgtgctaagg gaattagagc
    28381 aagaagggtt acttgttaat gaagaagacc gtgtttattt gacaccaaga gggacctttt
    28441 tggggaacaa tgtgtttgag cgctttttgt ttgaaaaata gtttaaagaa tatttgtttt
    28501 ttatctgaat ttaaataaaa aaagttcaaa aatacaataa aggactattg aaacgttgat
    28561 ataactggat ttacatcaat tatttagaat tagcactctt gtgtttagag tgctaaaaaa
    28621 tggagtcttt ctcttgacag gagaagggct tcttgctata tttataagtg gattagcact
    28681 tagaatatat gagtgctaat gagaggtgag gaagatgtta acaaagcgac aaaacgacat
    28741 tttacgtctg atcatccaac actatacgag tagtggcgtg cccgttggtt ccaaaacatt
    28801 gatgtctgag ggagtcaagg ccagctcagc aacgattcgc aatgatatga aagcattaga
    28861 agatgaagga ttgctgttga agactcactc ttcttctggg cggattccat ctgctttagg
    28921 ctatcgttat tacgtggatc atttgttaaa acctgctcgc gtagcagaag atgatttgct
    28981 gctgattcgt cagtctctag gcagagaatt tcatgaaatc aatgagatta ttaagcaatc
    29041 tgctgagata ttatctgagc tgacaagcta tacgactttt tcattgggac ctgaaataaa
    29101 agaccgccga ctgactggct ttcgtatcgt acccttgaat aatcgacaga ttattgctat
    29161 cgtcgtaaca gatatgggga atgtagaaag ccaagtgttc actcttcccg aatatatggg
    29221 aagtcaggaa ctagaaaaaa tggtacagat tgtaaacgac cgtctagtcg gagatcctct
    29281 gatgacggtg taccataaac ttcgtacgga gattccgatg attcttcata aatatttcca
    29341 aacgacagaa gggatgtctc agttatttga caccgttctt ggtcatgttt ttgaagataa
    29401 ggtttatgtc agtggacaaa tgaatctctt ggattatgag ccccgtcaag atcttgatca
    29461 gttcaaatcg atgttttctt ttatgaaaga ctcagacgga cttacacaaa tgatcactcc
    29521 gatggatagc aatattcata tcaagattgg tgctgaatta ggcaatgatt tattgcagaa
    29581 tatgagtatg attcaagcaa gttacgagat agatggtcat ggaagaggta caatagcctt
    29641 gttagggcct actagtatgc cttattctcg gatgttcggt ctagtcgatg tttatcgaag
    29701 agaactagca gctaaacttg ctgattacta ccgttcgctt gacttgtcaa actcgtaatg
    29761 tgaataacga agctatttgc agctgatcgt tctctctttc ttagtaagag caatcaaaca
    29821 gctgactgaa taatgaaagg aagactgtat cgtgaaagaa aatcaagaag aactagataa
    29881 agaaatgaca gatgctcagc cagaaccgga aatcgatgtc gaagctgcag aagattcggg
    29941 tatttcagaa gctgaagcgg aagaattcga aacagcgaaa ttaaaagctg agttagaaga
    30001 aatggaagat aaatacttgc gggctcgtgc tgaaatcgca aatatggcaa atcgaggaaa
    30061 aaatgagcgt gagcaattac aaaaataccg ttcacaagat ttagccaaaa aattacttcc
    30121 atcaattgac aacctggaac gtgcattagc cacagaagtg tcagatgatc aaggggcagg
    30181 tttgaaaaaa ggcgtggaaa tggtcttgga aagtttgcgt aacgcactgg aagaagaagg
    30241 aattgaaaaa atccctgcta aaggagaagc ctttgatcct aatcttcatc aagcagtaca
    30301 aactgttcca gcgactgaag atacaccagc cgatacgatt gtagaagtac tacaagaagg
    30361 ctacaaatta catgatcgcg tgttaagacc aaccatggtc attgttgcac aataattgtg
    30421 cggatataaa acttacactt tcaattaaag aaggagacgt taattatgag taaaattatc
    30481 ggtattgact taggtacgac taactctgca gtagcagtat tggaaggaaa cgaagcaaaa
    30541 atcatcgcta acccagaagg aaaccgtaca acaccttctg ttgtatcatt caaaaatgga
    30601 gaaatccaag taggggaagt tgcaaaacgt caagctgtga caaacccaaa tacgatttca
    30661 tcaatcaaac gccatatcgg tgaagcaggt tacaaagtag atgtagaagg taaatcatat
    30721 acaccacaag aaatctcagc aatgatcctt caatatatca aaggattcgc agaagactat
    30781 ttaggtgaaa aagtcgaaaa agcagttatc actgtgccag cttactttaa tgatgcacaa
    30841 cgtcaagcaa caaaagatgc tggtaagatc gctggtctag aagtagaacg tatcgtcaat
    30901 gaaccaactg cagctgctct tgcctatggt ttagataaaa cagatcgcga cgaaaaaatc
    30961 ttagtattcg accttggtgg cggtactttt gacgtatcta tccttgaact aggagacggt
    31021 gtctttgatg tattgtcaac agctggcgac aaccatctag gtggggacga ctttgataac
    31081 aaaatcatcg actacatggt agcagaattc aaaaaagaaa acagcattga cttgtctcaa
    31141 gacaaaatgg ctttacaacg cttgaaagac gctgctgaaa aagcgaaaaa agatttatct
    31201 ggcgtatcaa gcacacaaat cagcttgcca tttatcacag caggagaatc tggaccattg
    31261 catttggaaa tgacattgac acgtgcaaaa tttgatgaat taacagctga cttagtagaa
    31321 cgtacaaaag taccagtacg tcaagcgcta aaagatgcag gtttatcaca atcagaaatc
    31381 gacgaagtta ttttagtcgg tggatctact cgtatccctg cagttgtaga agcagtacgt
    31441 aaagaaacag gaaaagaacc aaacaaatca gttaaccctg atgaagtagt tgctatgggt
    31501 gctgctatcc aaggtggcgt gatcactggt gatgtgaaag atgttgtttt acttgacgtt
    31561 acaccattgt cattaggtat cgaaacaatg ggtggagtgt tcactaaatt gattgaccgc
    31621 aatacaacga ttccaacaag taaatcacaa gtattctcaa ctgcggcaga taatcaacca
    31681 gcagtagaca tccatgtatt acaaggtgaa cgcccaatgg ctgctgacaa caaaactcta
    31741 ggaagattcc aattgacaga tattccagca gctcctcgtg gtattccaca aatcgaagta
    31801 acattcgata ttgataaaaa cgggattgta aatgtatctg cgaaagatct aggtacacaa
    31861 aaagaacaaa aaatcacgat caaatcttct tcaggtctaa cagacgaaga aatcgaacgt
    31921 atggtaaaag atgcagaagc aaatgcagaa gcagataaag ctcgtaaaga ggaagttgac
    31981 ctacgtaatg atgtagatgc attgctattc tctgtggaca aaacattgaa agaattagaa
    32041 ggcaaagtgg acgaagaaga agtgaaaaaa gcagaaacag ctcgcgatga attgaaagcc
    32101 gctgttgaag caaacaacat cgaagaaatg aaaactaaac gcgatgcatt gaacgaaatc
    32161 gttcaaaact tgactgttaa actatacgaa caagcggcac aacaacaagc tcaagaaaac
    32221 ccagaagcag ctcaaggcgg agcagatgat gtcgttgatg ctgattttga agaagtcgat
    32281 ggcgacgata aataatcagt tcactgaatt tcgagagaag gccaaagttg ctactttggc
    32341 tttttctttt gaaaaatagt ttgaagtatc ggtttttgtt tcaaacaaaa tatgatatga
    32401 taacaagcga cgtgaaaatg atgagaacca ataggaggaa agcctatggc aacaaaacgt
    32461 gattattatg aagtcttagg gctctcaaaa ggagcatcag aagatgaaat aaagaaagct
    32521 tatcgtaagc tttcgaaaaa ataccatcct gacatcaata aagaacctga tgcggaagaa
    32581 aaattcaaag aagtatcaga agcatatgag attttaagtg acccgcaaaa acgcgctgca
    32641 tatgaccaat acgggcatgc aggcacagat ccaaattacg gtgctggtgg agctggtggc
    32701 ggatttggtg gattcggcgg cttctctggt ggcggcttcg gtggttttga agatatcttc
    32761 gactctttct ttggaggagg cggtcggtca gtcgatccaa atgcgcctcg tcaaggtgct
    32821 gatttgcaat atacggtcga cttgtctttt gaagaagcga tttttggtgt agaaaaagaa
    32881 atcaagtata atcgggaaga aatctgtcat acttgtggtg ggaacggtgc aaaaccaggc
    32941 acacaaccaa ctacttgtca taaatgtcat ggtgcaggta cgattaatgt agaacgccaa
    33001 acaccacttg gacgagtgat gagccgtcaa acttgtgatg tttgtcatgg gacagggaaa
    33061 gaaatcaaag aaccttgtcc aacctgtcat ggctcaggac atgagaagaa agcacattca
    33121 gttaaagtga atgtaccagc tggtgtagaa gaaggacaac aaatgcgttt agctggtcaa
    33181 ggagaagctg gagaaaacgg cggaccattt ggtgatttgt atgtggtctt ccgtgtagaa
    33241 gaaagtgata tctttgatcg tgaaggttct gaaatttact atgaattgcc tttaagtttt
    33301 gtacaagcag ctttggggga tgaagtccaa gtaccaactg tccatggaga tgtgaaattg
    33361 aagatccctg ctggaacaca gacaggtacg aacttccgtt tacgtggaaa aggcgctccg
    33421 aagttacgtg gtggtgcaac aggcgaccaa catgtcaaag taaaactgat cacaccgaaa
    33481 aatctaaatg aacaacaaag agaagcatta cgtgcattcg ctgaagctgg cggcatcaaa
    33541 gttgaagaac agcaagaaga cggtttcttc gataagttta aagatgcttt tggcgggaaa
    33601 aagcggaagt agtttttcta gtaaaaacgc taagtcatta ataattaaaa accagacgaa
    33661 caatcatatg ttcgcctggt ttttttgtag ttgtcatgtt tgtaccaaca aaaaagccag
    33721 ctttagaata tgataatcgt catagtttct aggggtataa ccagtgagag aatggaactt
    33781 gttcaatcga tattgcaagg tgtttttatg aataaataat tcttctgagc aatttttgat
    33841 gcttccgtta tttttactga agcatgccaa agtcttcttg aacaaatgta cttcttcttc
    33901 tgtcaggctt ttcaaaatac gttttacata taatgttcgt tgatctgacg ggattgctgg
    33961 taaaacgatc ccgaggtcta aatcttcaaa aaagcggatc tcgttcacag attgatggaa
    34021 agctatccat tcgcaaactt gtttggcatg aaggaaagat tgcggtgtct cttgctctgt
    34081 ataacaaagc gacccaattc caaagtttag ctgtaactga tacattttat taaactttgt
    34141 ctggatttta tcaagcaatg atagcaatga ggctctggaa atccgattga ctaggataat
    34201 gatttgttgt ctatgtacag tgagcaaagc ctgtccgtaa taagagaaga ccctttcgag
    34261 caactccaat aaaggattga tccgttcttg acacaattgc tgattgtcag aaagttgtcc
    34321 cacaactaca taatatggag aagaaaaatc aagctcaatt aagtgagggt ggtatcgact
    34381 atgtacttga tatcatcaat gaaccgatgg tactttctta tgcgatggaa caggtagagc
    34441 gattgcttgt ttgtgcagga aaacaagcga tgcggctgat acgattaggt gagaaaataa
    34501 gaagataaaa atacacggtc ccttcatggc gaccgtgcat ttttggtttt tattaaattt
    34561 tatatctaat tttcaattat ccgcataaac actatacatt caagcatttt gaagtataga
    34621 aaaacactca tcttaccacg aatttaccac gattgaatga gccttgatat gaccgtaaaa
    34681 ctactaaggg agataacacg aaactgtgta tctcccgatt tttttataca gttgattact
    34741 tcactccgat ttcaaatttc aatattgctt tcatcatgtc tgtatgtatt tctcctttta
    34801 gttcttcatc aacctgcata atgattttac cactttcaga gtggtaaaaa ggacgcaagc
    34861 ataatttaga aatataaccg tcataaaact tcaaaatctc tttaactgcc atttcatctc
    34921 cgcttgacgc tttgcaaatc aattcaaaag agggatagcc ataatcttta cttcctttcg
    34981 ctcaaaagca tttttaactt ccttagtcct ctagttcttc tactgcaaat agcagaacgt
    35041 gatacatggt acagttctga aatttctctg tcgttcattc cttgaaaata acgtaataaa
    35101 atcgcactac gctgtttttc tgatagtcta tgtaaaactg taccaagtgt ttcatcagat
    35161 acataaattg taaagtgcaa gactttaaat gatacacaat cataaaaaca ggtatcaatc
    35221 gttccacgtt caacttgttt cgtttcggct atttcacaaa acaatacttc acgtttagaa
    35281 tgtcttgcaa gctgtctgtt tcgacttttc actttgttct taatgacaat cagcattaaa
    35341 gaattgaact gtaatctgac gatatgctcg aaagaagatg tcctcataat ctcaccccct
    35401 ttctgtctag gaaaggaagt gagccttgct cgtttatctc ctttcactct tagtcccaat
    35461 gtgaaagggg gatttgttgc attactgata aataaacttt gtaaaaaagt tctgaatagc
    35521 caaaaaagca tataaacaga tttatttctc tgtttacatg cttctgttat tctatctata
    35581 tgatttataa aaccacattg gtggacgtac ttatctattg cagatagacg actttttttg
    35641 acaagaaccc aatgtaagga aatttattgt atatgatgta cttcatggcg acgttgacct
    35701 ccaacaaacc gccatttgga agtaatatac aatattttaa cagcgtaaat agcactacca
    35761 tataacggtt ttttttattg gcgtttagta gtgcttttta ttaaatataa acctataaac
    35821 catataacac gtttttctat acctgttttt aattcagtag gaacaataaa atgtatagag
    35881 gtggtctact atgcgtaaaa aagaagataa atatgatttt agagcctttg gtttagccat
    35941 taaagaagct cgattgaaac gaggtttaac tcgtgaacaa gtgggagcat tgattgaaat
    36001 tgacccacgg tacttaacta atattgaaaa taaagggcaa caccccagca tacaagttct
    36061 ttatgacctt gtatcgttac ttcatgtttc cgttgatgaa tttttcttac ctgctaataa
    36121 cttggtaaaa agcacccgac gattacagat agagaaatac atggatagct ttacagacaa
    36181 agaactatcc ttaatggaat ctttagccag cggtatcaac gaagcaagaa acatcgaaga
    36241 ctaattaaaa gaatccatac ataacggaaa gagccgataa aatgagattg tattaatctc
    36301 attttatcgg ctctgcgtct ttgcgtctgg ctctgtaatc acagttactt tgaactgctt
    36361 tatttcaatt aaattttctt gtctgcattt cggacaatag agggggaatt tttttaattc
    36421 agtatcttcc cttatcttta atcgtgtttt atttccacat acaggacaca atatccactt
    36481 gtagtttata ataactatct cctcctttac actttaattc aaatctttat taaaaaatat
    36541 ttcatcttat ttaacaagaa accatattta tataacaaca taaaatacac taagttattt
    36601 tattgaacat atatcgtact ttatctatcc gactatttgg acgacggggc tggcaaacag
    36661 gttcaccggt agtaacatgg taccctttta actctgttaa acaaacacta cgtccatttg
    36721 taaagaaagt taaatcacta cgatattctt gaatacaccg agcagggatt tctccactaa
    36781 gaatgacctc attatttttc aattgagtgt ctacgatgtt cgcacaatat ttaggagcat
    36841 cgttgtatgc tcgtgaaaga tattcctgtg gcgcataaat tttaaaacta agatatggct
    36901 ctaacaattc tgttccagct ttttttaaga cttgttccaa tacaatagga gcaagcatcc
    36961 gaaaatctgc tggggtacta acagggctat agtataagcc atacttaaaa cagattttac
    37021 agtccgtcac attccaacca tacaatcctt gttcacagcc atagcgtatc ccctccataa
    37081 ctgcattttg aaacgattga tttaagtatc caagagaaac cgagctctca tactgcattc
    37141 cacttcccaa cggaagcggt gatacagata aaccaatgga agcccagaaa ggatttggcg
    37201 gcactt
//
LOCUS       DACDIE010000024        35927 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.024, whole genome shotgun sequence.
ACCESSION   DACDIE010000024 DACDIE010000000
VERSION     DACDIE010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35927
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.024"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(37..1188)
                     /locus_tag="IV250_001296"
     CDS             complement(37..1188)
                     /locus_tag="IV250_001296"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAP8768590.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(1201..1638)
                     /locus_tag="IV250_001297"
     CDS             complement(1201..1638)
                     /locus_tag="IV250_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8768591.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(1653..3419)
                     /locus_tag="IV250_001298"
     CDS             complement(1653..3419)
                     /locus_tag="IV250_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAP8768592.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            3683..4360
                     /locus_tag="IV250_001299"
     CDS             3683..4360
                     /locus_tag="IV250_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8768593.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            4363..5490
                     /locus_tag="IV250_001300"
     CDS             4363..5490
                     /locus_tag="IV250_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8768594.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(5690..6130)
                     /locus_tag="IV250_001301"
     CDS             complement(5690..6130)
                     /locus_tag="IV250_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8768595.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(6141..8174)
                     /locus_tag="IV250_001302"
     CDS             complement(6141..8174)
                     /locus_tag="IV250_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP8768596.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(8176..9879)
                     /locus_tag="IV250_001303"
     CDS             complement(8176..9879)
                     /locus_tag="IV250_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8768597.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(9973..10923)
                     /gene="manA"
                     /locus_tag="IV250_001304"
     CDS             complement(9973..10923)
                     /gene="manA"
                     /locus_tag="IV250_001304"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAP8768598.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            complement(10962..11987)
                     /locus_tag="IV250_001305"
     CDS             complement(10962..11987)
                     /locus_tag="IV250_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP8768599.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(12196..13065)
                     /locus_tag="IV250_001306"
     CDS             complement(12196..13065)
                     /locus_tag="IV250_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HAP8768600.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(13524..15416)
                     /locus_tag="IV250_001307"
     CDS             complement(13524..15416)
                     /locus_tag="IV250_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8768601.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(15413..17140)
                     /locus_tag="IV250_001308"
     CDS             complement(15413..17140)
                     /locus_tag="IV250_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8768602.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(17156..17806)
                     /locus_tag="IV250_001309"
     CDS             complement(17156..17806)
                     /locus_tag="IV250_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8768603.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            18010..18306
                     /locus_tag="IV250_001310"
     CDS             18010..18306
                     /locus_tag="IV250_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP8768604.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            18478..19098
                     /locus_tag="IV250_001311"
     CDS             18478..19098
                     /locus_tag="IV250_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAP8768605.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(19190..20356)
                     /locus_tag="IV250_001312"
     CDS             complement(19190..20356)
                     /locus_tag="IV250_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP8768606.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(20368..20721)
                     /locus_tag="IV250_001313"
     CDS             complement(20368..20721)
                     /locus_tag="IV250_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP8768607.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            20849..21871
                     /locus_tag="IV250_001314"
     CDS             20849..21871
                     /locus_tag="IV250_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HAP8768608.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            complement(22310..22744)
                     /locus_tag="IV250_001315"
     CDS             complement(22310..22744)
                     /locus_tag="IV250_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8768609.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(22854..24713)
                     /locus_tag="IV250_001316"
     CDS             complement(22854..24713)
                     /locus_tag="IV250_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP8768610.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(24831..25991)
                     /locus_tag="IV250_001317"
     CDS             complement(24831..25991)
                     /locus_tag="IV250_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HAP8768611.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(26298..27722)
                     /gene="araA"
                     /locus_tag="IV250_001318"
     CDS             complement(26298..27722)
                     /gene="araA"
                     /locus_tag="IV250_001318"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAP8768612.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(27738..28436)
                     /locus_tag="IV250_001319"
     CDS             complement(27738..28436)
                     /locus_tag="IV250_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP8768613.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(28393..30054)
                     /locus_tag="IV250_001320"
     CDS             complement(28393..30054)
                     /locus_tag="IV250_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HAP8768614.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            30272..31672
                     /locus_tag="IV250_001321"
     CDS             30272..31672
                     /locus_tag="IV250_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAP8768615.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(31924..33003)
                     /locus_tag="IV250_001322"
     CDS             complement(31924..33003)
                     /locus_tag="IV250_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8768616.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(33089..34048)
                     /locus_tag="IV250_001323"
     CDS             complement(33089..34048)
                     /locus_tag="IV250_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HAP8768617.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(34060..35583)
                     /locus_tag="IV250_001324"
     CDS             complement(34060..35583)
                     /locus_tag="IV250_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP8768618.1"
                     /translation="MDEEVKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHE
                     TADGDGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIE
                     PNSFGLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQ
                     KKPFGVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPR
                     LPSYPEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFD
                     RQIEEIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMED
                     TLLFGSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAK
                     GQVLKQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLL
                     DQGEHYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(35593..>35927)
                     /locus_tag="IV250_001325"
     CDS             complement(35593..>35927)
                     /locus_tag="IV250_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8768619.1"
                     /translation="LTIIIYFLVNQLLEEQTIVLSISGYVVLLIYSMFLLSWIYVISE
                     NKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIFLFPPFIFIFAGGVWKLVSI
                     ILTIGNER"
BASE COUNT        10195 a         7530 c         6305 g        11897 t
ORIGIN      
        1 aaaatttcgt cacattcccc tttttcgtat cctattttaa gcgacttgct tggcataacg
       61 gttcacgttt tgcttgatat tcccgattgt tttcggatca ttgattcctg tgacttcttg
      121 aatcaattgt tccaagtttt catggatctt catttcatgc aattgacggc tttgttcatc
      181 ttcaggatca tagtaattaa aaataatccc cattgttgcc gtcaaatgtg ggcaagtcaa
      241 gttatgttct tgtagttctc tcactggacg tgtaaatctt tcttgataac ctaacttacg
      301 taatggtgtc cgagcgacac gtgtgatcgc atcagagata tttttgtttt ggaagcgatg
      361 aatgattttt tcaatatacg catgatgctg ctctgcatca aacccccatt tggcaatcag
      421 caaacttcct gtttcctgca aaactgattt taattgtgcc acaactaacg catcttgcat
      481 tgcttcatca atggtctggt aacctaacaa ggcacctgta taagcgacag tcgcatgacc
      541 agtattgaca ctaaatagtt tgcgttcgat atatggttct agatcatcta catataaaac
      601 cccttttaat tggatttttt tattttttct ttggctatcg tcaatgaccc attctttgaa
      661 aggttccact tggacgaata aaggatcttc atggtgttgt aatgggacga ttcgatcgac
      721 tgctgcattt ggaaagccga tatatttgtc agtaaatgat gtttccggaa gatatttata
      781 cacttctttt tctagaaatg ctgaaccgcc aatcatattt tcacaggcaa tgatatctaa
      841 agatgttgtg cttccttcct tttctctttc ttggatacct tttgcaatca actcagcaat
      901 aaaaggtaag atattcgggc caatggcggt agtaactaaa tctgcttcag cgatttcttg
      961 gattactttt tctggatttg tttgattgtt gattcctttg acatttttta cagtgatttt
     1021 ttcttttgat tcatctgcta attcgatttc gtattctttt ctttcattta aagcatcaat
     1081 gatcgtgtga ttcacatcta cgaaagtaat ctcaaatcca ttagctgcca aaacctctcc
     1141 aataaatcct cgaccgatat ttcctgcacc aaaatgtact gctctcattt ttctctctcc
     1201 ttaagcaatt gctagatttt ccgttacttc ttctttgctg agcgcgtctg ctagctggac
     1261 aacattgtcc atatcactgc aataaagtgc gatttgctgt acaagttgca aatgctcatc
     1321 tccgactcct gcaataccga acaaaacagt cgcaatcttt tcttcttctt cggtaccaaa
     1381 atctacacca tctggtactt gaacgacaca gatgccggat tttttcacat atttttttgc
     1441 ttcatctgtt ccatgaggaa tcgcgataaa attccccata taaacagata acattttgtc
     1501 tctatctacc atggcatcga tatattcctc atcaacgcac cctgcttcta caagcatctg
     1561 accgcaatag cggatcgctt cttctttggt tttaaaagat cgattgagct cgatcatgtc
     1621 gaatggcaaa ctttgcatgc tgttttcctc ccttaagctg tctgtttcaa atattgtgca
     1681 atcgattcgt atttttgcgg attgaccatg ctgttgactg ataaatgaat ggattgaggt
     1741 gcagccatct gtactttttc cgctaaactt tctttggcta cgatcagtga atcaggttca
     1801 tcttttaaat cttggatatt gacttttctt aacgggatag ttaaatggtt cttgtctaat
     1861 atatctttga gcacggtcat ccccattgtt gctgatccgg aacgatcatc gtggacaagt
     1921 agaatctctt tgatttgttt gatgtttgct gaaagaaact cttctgctgg ctcattctca
     1981 atttcagaaa ctgatgtttt ctgcttgctg cttaattctt gaatgatttc atcatatcga
     2041 ggtgaattca aaaagttttc tactgagaca aaacgcgcgc tcggtgcttt ttgttttgct
     2101 ctttcttgta gttcgacctg agtaacgatc aatgtattcg agtcatctgt cagttggctg
     2161 attgcttgat tggtcaccgt ttgaggcaag tctgcttcct gtactttttt tctcaagatc
     2221 gaagcgccca tcgcacttga tcccattcct gcatcacaag caaaaatgat tttttgtacg
     2281 ccagagaatg tagctccttg aggaatacct tgggcacctt tggaagcttg cttagctgcc
     2341 tttgtctctt gaacagattt ttcgaagtct tcatttgtat ctttcgcatc cgctttcaaa
     2401 attactgcag aaataccaaa cgaaacagct gcggcaacaa tcacgcccaa aataacagga
     2461 aggtaagaat tgaacggtgt catcgctaga atcgcaatga tcgaccctgg agaagctggc
     2521 gctcttagtc cagcatccat caattggaaa atgaaacttc ctgacattcc tccgcctata
     2581 actgataaga ataataacgg tttcatcata acgtaaggaa aatagatttc atggatcccg
     2641 cctaaaaagt gaatgatcat ggctcctggt gctgaagatt ttgctgctcc tttgccaaac
     2701 aaagtaaaag caagtaatac gccaagtcct ggaccaggat ttgcttcaag caagaataga
     2761 atagattttc cagcctctgc tacttgttca gttcccaatg gtgtcaaaat cccatgattg
     2821 attgcattat tcaaaaataa gatttttgct ggttcaataa aaatacttgt caatggaatc
     2881 aaatgggcag ataaaatgat ctctacacct tttgccattg cttgagtcaa tgcatcgaca
     2941 actggaccaa tcgcagaaaa acctaataac gctaatccaa aaccgataag acctgctgaa
     3001 aaattattga ctagcatttc aaaaccggat ttgatccgtt tttggaatag ctgatcaaat
     3061 ttcttgatgg catatccacc aagaggtccc atgatcatcg cgcctagcat cattggaata
     3121 tctgttccga caatcacacc catcgttgca attgccccaa caactgatcc gcggtcacca
     3181 gcaattactt ttccgccagt ataaccgatc aataaaggaa tcagataagt tagcattggg
     3241 cttaccatgg tagccaaagc ctcatttggt aaataaccat ctgggataaa tagagccgtt
     3301 aatacacccc aggcaatcag tgctccaata tttggcatga ccatgcttga taaagtactg
     3361 cctaattttt gtaccctcgc ttttagcgaa atgttgtttt tgggaatcgt ccgttccatt
     3421 ttgtctctcc ctctctcctt ttgatactct aattgtatcc gcttactaaa aagagaaata
     3481 gtcgtacttt ggcacgaaac tttgtgccaa aggtttgagt atgtcagcaa aataaaaaga
     3541 tttcggtatc agaaaacttc aattttaaat aaaactgtta ttttaatttt tttataatat
     3601 gcaaacaata acttttgtta gcatttaatt tagatgtttt ccattctatt atgttattaa
     3661 attttatatt ggaggcctat ctatgacatt actgatcaaa aacatgaaca tgaaacgtgg
     3721 taaaaaacgt gtgattcgac aattgaatct tttagtagaa tcaggagaag tagctgcttt
     3781 gattgccccg aacggatttg gcaaaacaac tttgctgaat ggcattgccc aactgctgcc
     3841 aacaacttat gatcaattgt cccttcacaa catctctcct actgcttata cagattatcg
     3901 ccggtctttc ttttttctag aatcaacgaa tcagttatcc gactatctat cggctgttga
     3961 ttatttgact ttgatcaaat actattggca gagcaaagta gaatgccaaa ctgttttcca
     4021 acgattgaag atgactgagt accttacttt gccgattgga aaaatgtctc taggaatgaa
     4081 acaacatgtc ttgcttgctg cttacttgat cagtgattca tctgtcatgt tattcgatga
     4141 accgttgaac ggattagacc caggaagtat tgatatcttg aatagcctca tcagacaatg
     4201 gcagaaagag gggaagacga ttcttctttc ttcccacgct atcgcgaata tccaatcgat
     4261 ttgttctaaa gcatgttttc taaaaaatgg agaaatcgtc acagaatcct caaatatgga
     4321 agagatttta gctatttatc gtaaattata tgaggtgtaa ctatgtcttt acttacattt
     4381 gaacgcaatc tcttactaaa aaacaaagta aactttttat ttcctctatt gttggtcgtg
     4441 ctctttgcat tcccactttt ttttgatcat aaacttgctt atacagaatt cgatgaactg
     4501 aatcataact atgaagagat gcaacgtttg attgaaacgt tgaaagaaga tgagaatgag
     4561 aaagaatttg tggaaagttt agagaaaagc aacaaactga tcgaagcaat cttacatgca
     4621 aaaaacacag ggaatgtcca gcaaatggtc gaagcaactt atcattatga aaaagatata
     4681 ctggatcgac tgatttctgg tcaaagacaa ggaattccaa tcattgaaca acaaaaacgt
     4741 gtcgagttgc tccgttatat gaaagaacat cagatccaaa gatattctat ttttgacttg
     4801 ccagcgcatc tccctttagc aaattactac gaaaatattt tttcaggaat gatttctagt
     4861 tttttgattt tatgtataac cgccttgttt ctttcttcca tcatctctta tgaaaaaaga
     4921 aagcaggtaa tctctctagt caatctatta ccggatagta tggtaaagaa acatagcatc
     4981 cgttttacca tttattatgg tgcagctatg ctaagtctcg tgatgccatt tttgatcgtc
     5041 tctatactag taataatcaa aaatggtttg ggagacttcc gttatcctgt aggaacgatc
     5101 attggtcagg agatacgaat ccttccgatg tatgagtatc ttttccagag ctttctgttc
     5161 ctgttgttgt gggtgctctt tttaagtaca atcagttttc ttctatctgc tttatttgaa
     5221 cattctctag tcaatctttt aggtacactt ctttgtcttt tcttagcaga gtatcgcttg
     5281 ttttcttcga ttggatggat agaatcaatc agtcattact tgccaacttc ttatgttgat
     5341 tttcaaaatg tgatcatagg aggagatatt ttctcgccac tagcttcaga acaggttact
     5401 ttcatgaatg gtatcttgac acttggtata tggagtatcg ttttattatt cataggtatg
     5461 ggcactatct atataaaaaa gagctattaa aacacaaaaa taataggggc ggtgacaaaa
     5521 accttgtcac cgtcccctat ttcaaataaa cggttttcta gaataaatag ttttcaaaag
     5581 cttgcatccg cagtaataag aacctcccaa caaaaatagc tcctcttatt tttatcttgg
     5641 ttgaagttat tacttgatgc aaggcagctt tttttactgt gaacattggt tatatatgtc
     5701 catgttcaaa aatcatttgg acttcttcaa tggttttcgc atcactgagc gccattactt
     5761 ggtcgatgtc tgaacagaaa aaggctaaat cttggagcac cgctaactga tgttcttttt
     5821 ttaaagccac tacaaataag agtgtcgcga tttttggttc ctcttttgtt ccaaagtcca
     5881 caccatcagg cacttggact aaatagattc cttcttctac tacttcttca tctttgtcag
     5941 tcccatgtgg caaggctacg aaattcccga tatagacact cgctttttct tgtctttcga
     6001 tcattgattg gatatatgcc ggtttgattc ccccttgttg ggtcatccac tggccgatct
     6061 cggtcaacgc ttctttccaa ttttgatagg attgatcgag caatattttt tctgctggta
     6121 gctgtatcat cttttcacct tcatttcttt atttcttcaa tcaggatttt cgctaacaac
     6181 tgataaacga tttctttatt tcctgagcgg aagatctcgg tgttcaagtc attcatgatg
     6241 attgctccac tgatctttcc taaaagaatc ccgtcttttt cttggattgg cgaaggcgcc
     6301 agcataacta acatacggga caaatcgact ggctgtctat ccatcccttc aatggtcaac
     6361 ggatgtttca aattgaaaat acaaaagatc ggtttagaaa tcaatgagct ggatgtatgg
     6421 aataacccca tatttgtatg aggaataccg ataggtgctt gttcatatcg cttaagcagc
     6481 cggcgatgaa ccacctcttc atccgttact agtccttcag gaaaataagg aaataagctg
     6541 cgaatcgttt cggaaatcgt ctcaggatta tccagttctt ggataaaaaa acgaccgagt
     6601 aattgattga tttgatccat gaaatgcatt gtttcctcat aagtatcttc tatcatccac
     6661 tcgttattgc tgttatacgt aattttttgc ctttttttct tctcaatatg cgaaaaagct
     6721 tcttttagct gcttgacttc atcttctagc agtagcgggg agactaaaat atattttcca
     6781 ccgtatcctg gtaaaataga tgtagaaata acaacatcat attcttcttg taaatcgatt
     6841 ttttttaaat cagcaatccg atagaaatga atttgttgga tgaagggaaa ataccttttc
     6901 aatcgtagtt ccaacatgct agtagaagct aatccacttg gcgaaaatcc tgcaatactg
     6961 atattgcttt ctttcggact tcgttccaat gaattcgcaa aatgaaggac catataagcg
     7021 atctcttctt cggataattt tttttcttga aaaatccgct ctactgcttt acggattgca
     7081 ttcgcaactt cctcgtattg aaccatgatt ttttctaaaa tcggattggt cagtgtctct
     7141 tcttgtaaaa cggctcttga aaacacacca gataaatgag tcaacagcat tttgtacaaa
     7201 gtttcatctt cataaaaatg gacattcgtc tcttcactta cttcttgaat cagttgtttt
     7261 actaaataag caagctgtgt atcgaacgtc tcttcaaaga aattctgatc aaatgagttc
     7321 gtgaagtcat ttaataatcc tgcaaaaaag acgatctcat ttactggata aagttgtttt
     7381 gtctcagaag cgatccgtga aaacaatcgt ttagctgtct gtagtaattc tttgtttgcc
     7441 agactattat atgtctcatt tgtcagtaaa tgatttttag tcacacgctc catcgtcaat
     7501 gtcagcatca aaaccaaatg ttcaagcttt ctatcactta gattggtctt tttttggatc
     7561 aattcttccc cgattacttt gcgtgcaaga gatagatgtt gcggatcaac gaatcgaaaa
     7621 aaataattca gtgatgcatc gaaagaaata ctatgaaaaa gttcatattc attgatttct
     7681 aattctaata cattcgctgt cagaagccgg cggtattttt ccggtccttc gatctcgtat
     7741 ccctggttcc gaatgatttc caaaggcagt tggcgaatac tttcttccaa ctgtttgata
     7801 tctgcataaa aagtggtatt actgatgcga tatgattcca tgaaatgagc aagactcaac
     7861 ggctctgtcg tcagtaacaa ctcggctaaa atcgcgtgtt gtcgtttagc agtggataag
     7921 tcattacttt tcccatctgt attttctttt aattgctcta atacttcttc tgatgcctca
     7981 atgacaaatg agcctctagc tgcttttttc aaagaagcat ttaccgattt caatgagact
     8041 tcaagattat ctaattcgcg gtaaatagtc cgtttactga ccttcaataa attcatcatt
     8101 ttttggatag atacaggtga cgggctactg agaaattctg ctaataattg tttttctctg
     8161 ggcgataaat acatcttata tttctccctt cacgagtttt tcgtattcat tcgtagctac
     8221 ccaatgttca actagaacta gccgagggat cttttcttct agctgttcag ttacagctag
     8281 ttctctttgt gcgatataaa tcgtttcttc ttggattttg attttttcta ttgattcttt
     8341 tgagataaca aactgttttt tcgctttttt tgctaattga cttaaaatcg tcatgcccat
     8401 agtttgtgag ccgcgtctgt tgtttttata caaaaataca atattagcag gcgacttttt
     8461 ttgttctgtg atatctgcta tctctttttt ttcttcccgc tttggaaatt ggatcgcttc
     8521 ttttattcgt gacagcaaga tatctgtttc caaaaagtga tccagtacat agatttgact
     8581 ttgaggtact ttttctcttg caagacccgc tagttcatgt tgcacgataa ccagctgatt
     8641 tacttggtta ttcaattcat agatagagct gtatccgaca gaaagatcga ttttttctgc
     8701 tgctaatttc tttttcagca gggcggctcc catagcactt gatcccatac ctgagtcgca
     8761 agcaaaaatg acggaatgaa tgatagatgg ttccaagatt tctccccttt ctttgaccgt
     8821 tttgttctct ttttctttat ttggtaattt caatagtatc gctgcacaaa tgaaggtcac
     8881 aaatgtactg attgctatcc ctatacttac cccagctagt tgtggataag gcgcattggc
     8941 aaaaatcacg agtagtgacc caggcgatac aggtgcagct aatcccgtat ctaaccattg
     9001 aaatatcaac gtgccgcttg ccccaccagc cattacagct aaaaatagat acggtcgcag
     9061 caagacaaat ggaaaataga tttcgtgaat cccgccaatc gcatggatca tcaatgctcc
     9121 actcgcattt actttttcct gtttttctgc aaaacaaagg atagaaagta agatccctaa
     9181 accaggtcca ggatttgcct ccatcaaaaa taaggaagag tacccgactg tttgtgagaa
     9241 ttcgattcct aaaggtgtca atacaccatg attgattgca ttattgaaaa atagtacttt
     9301 tagaggttct aagatcagac tgacaagtgg taacagattc attgtgacta gccaagacac
     9361 aacggaggcg agcaacaagt gaacactatt taaaactggt tcaataaaaa cgattccaat
     9421 tatacaaata acgctaccag tcagtcctac tagtaggttg ctgacaagca tttcatatcc
     9481 agccttgaat ttcacgtgga atctagaaac aaattgttca tatacgatgc cggcaagcgg
     9541 cccgataatc atcgctccta cgatttgtgg agcattggaa cgagcgatca accctaaaga
     9601 agcaattgca ccggcgactg cccctttttg tccccctacc gatttcccgc ctgaataact
     9661 gatcaagatg ggtaaaagca gctggatcat ccaatggcat acctcttcta atgtggtgtg
     9721 caaatcgcca gacacacctt ttgttgctaa tgacaagatt ccccaagcga taaacagact
     9781 tagatttggc atgatcattt catttaagtg atgtccagct tttctcagtt gtttcataga
     9841 cacgcgactg ctgttgtttg ctttccattt gtattccatc tttttcacca tacttctatt
     9901 tattgatgga cggattttat gtagaaaaag cagcaaacga aaaaccgtct gctgctttca
     9961 gctatctggg aactatttgc ctggttcagt cgcaatgatt gctagttcac cttggaccgc
    10021 ccactcagaa acgccatttg gaataataca gcttgttcct ttttctaatg gatatgtttt
    10081 tccgtcaacg acaaggtctc ctgctccttc gattaccgtc atcaatgtat aaggggcttg
    10141 ttttttgaaa gatgtgattc ctttaatatc ccactcatat acattgaaaa actcagtttc
    10201 taaataagtg acgatcgatg aattcccttt tcggacttct ttgatttgta attctggtgt
    10261 tttcgcaggg acagttgtta catcgatcga ttgttggata tgcagctctc ttgtttgtcc
    10321 ttgcgcatct tttctgtcgt agtcatacac gcggtaagtc gtgtcagaac tttgctgtgt
    10381 ttctaaaatc atgattcctt taccaatagc atggatcgtt ccgcttggta cataaaagaa
    10441 atcacctttt ttaactggta ctttcttcaa aaggtcatcc cagcgcccat ctttgatcat
    10501 ctcagctagt tcttctcttg tctttgcatg atgaccatag atgatttctg caccaggttc
    10561 agcatcgatg atataccagc attcagtttt tcctaattca ccttcatgtt tcatcccata
    10621 ggcatcatct ggatgtactt gtacagatag atcatcttct gcgtctaaga tcttgatcaa
    10681 taatggaaac actggttctg aaggatggcc aaacaattcc ttgtgttctg accatagttc
    10741 atctaatctt ttccctttga attcgccatt ttctactaca ctgacaccat gaggatgcgc
    10801 actgatcgcc caatcttctc cgattttatc gttagggata tcaaaaccaa agactgaacg
    10861 taaacgactt cctccccaaa ttttttcttg aaaaactggt tttaaaaaca taggttcctg
    10921 cataattcaa actcctctca tttttcatag ctattatagc attatttcgg taataccgct
    10981 ttctctttca aaaagttttc aacaatttca tctcgacata tgagatactc cgcacgatga
    11041 tcgacaggat gtacgcgatt tgacaaaaat atcaacgctt cttgtgtttt tatatcaatc
    11101 aagaaaaacg tacctgtata accagtatga aaaagaatcg catgaccgtc ttctacatcc
    11161 ttttttaagt cccatcctaa agaacgatag ccattttttt tcggtgtttg atcttgcagt
    11221 aaagaatcaa tcgtctcttc ttctaaaaaa cgaccattac ggatttttcc cttgttcaaa
    11281 tacatttgga caaaagtaat gctgtctttc aaatcaataa agagacccgc gttacctgca
    11341 tgttcagcca aaactcttgc tttcggatca tgagtttgtc cacgcaaaac ctcacccgaa
    11401 ggcagttgtt ctgtgggaac gatttttttg gaaggaaaag gaagaaataa gctgttcatc
    11461 attcctaatg gttccaatac ttgttcttga aagatttcca ctgcagtttc ttggtaaatt
    11521 tcctctagca tcaacccaag aagaatcgta ccggcatctg tatattttac ttcttttcct
    11581 agttgctcac ctggttctag cttcaaataa gcggcgatca attcttcctg agatagctta
    11641 tctctgtttg gtatccatgt tttgatatca gaagtatgtg tcagtaaatg acgcaaagtg
    11701 attgtctgat tttcaaattc aggaagatat ttatgaagcg gttgatcgag ttctatcttt
    11761 ccatcttcta atagacgtaa aactaatgaa gtggttgcaa cgactttcgt cagtgaggct
    11821 gcatcaaaca acagatcttc tgtcatcagc tgcttttcag gaacgacttg ggcaaagccg
    11881 gctgtaaatg tttctacttg attttctttt atgaaggcga ctgctacacc tggataaaat
    11941 ccttcttcca tctttttcaa aataagctgt ttcgtgtgtg gatacatctt tttctcctgc
    12001 ttttctatca gattcttaca gtccaagctt aaagcttaat ctatgaggaa tcaacctttt
    12061 ttacacaaaa aaactgcgcg aaaaaccgcg cagtttcccc cggtatacta ttttctcttt
    12121 ctatctctag aataagcgat agtatgaccg ttcataaaat gacgagacat gcgacaatcg
    12181 ttaagaaaga aaaagttaag cgtcgcatcg aatgtcagtt ttattctttt cttttggctc
    12241 actgacgaac caccattcga ttcctatagc atcatagatt gtgagtactg tgtgcttttt
    12301 gtctaagaag aaatcaactg atagttgctg caagtgagca atcagtcctt ccatgctttc
    12361 agtatcgatc atgaatttca gaaaatcaag tccaatcact tcatctgtag gcaaatcaat
    12421 tgcgcctccg ctagcttctg tcaaaccaac atggaaatct ccttcattca atacatggat
    12481 gccctcttct tcattatgta ccttcaaacc aagtacatga ttaagaaatt ggcgttcttt
    12541 tgttaagtta gtaacattta aatggatttt ttcaaaataa gaacctgttg atagtttact
    12601 gaatttttct tcagattttc ttaataatgc ttcttgatcc attgtttctg gaatttctct
    12661 ttggtcgttc ttattctctt catggtaaat ttccaattca ttctcttctg ggtctacgag
    12721 caaaatcccc atacgtccat gatcttctaa agcttctttg attggataat tcattttacg
    12781 gatacgacaa aggatatctg ccatttcttc tgtgctaggg ataatcaatg aaaaacggtt
    12841 cagctttttg atcaccccca tgtgatcatt cgctctagga ctttcttcca gcagcaacat
    12901 ttcacttcca gcttcttctg ttcctaaaat ggctaattcg ttttcttctc ttttcaaatc
    12961 aaagccaaca acgttcttgt aaaaatcaat cattttatct cggtctttca cccgaatggc
    13021 tacacttttt aaataggtgg cagcccccaa ttgaaaatct ttcatatttc ttgctcccct
    13081 ctacgtcaag ctttatttta acgatggatt cttcttcttt caagcaataa ctttgatttt
    13141 gttaaaaaaa atgtgagaaa attcgcattt tgcttgtttt ttgtaaatta atgagtaaaa
    13201 actcttataa aaagaggttg tgaaaaagct gttctgcatc aagtaataag aacagccaaa
    13261 caaaaatagc tcctcctatt tttcgtttgg ttgggcttat taccgcagat gcaagctttt
    13321 gaacaccgtt taaggaagag gttgtaaaat cagcggtttg acctgagtat tagagataca
    13381 aaacggaaaa atagcttctc atattttgcc gttttttgag ctaataccga aggacagctg
    13441 attgaacacc gtttattcgg aaaaagggac tgtgacacga cttttgtcac agcccctaaa
    13501 aaaaataata aatttgtact attctatgaa gcttcttctt cagaaaactg actgttataa
    13561 agatctgcat agaatccgtc tttttccatc aatgtatcgt ggtttcctgt ttctacgatc
    13621 gaaccatgat tcatcacaat aatgttgtct gcatcgcgta ttgtagacaa gcggtgagca
    13681 acaacgaaac ttgtccggtt ttccaatagg cggttcatcg ctttttgaat cagtatttct
    13741 gttcgtgtat ctacactaga tgttgcttca tcaaggatca ggacatctgg atttgctaag
    13801 aatgcccgcg caattgtgat tagctgacgc tgcccttgcg aaatattgct tgcatcctcg
    13861 tttaatacag tgttatatcc ttctggcaat ttacgaacaa aatcgtctac gtgagcagct
    13921 ttagcagcct caatgatttg ttcgtctgtt gcatcgtcgt tcccatattt gatattgtca
    13981 tagatacttc ctgtaaacag ccaagtatct tgcagtacca ttgaaaactg tgcgcgcaat
    14041 tcatcacgtg aaagatcacg ggtatctaca ccatcgtaac gaatactacc tccagaaaca
    14101 tcgtagaacc gttcaagaag gttgatcagt gtcgtcttcc ctgcaccagt cggacctaca
    14161 atagcgacca tctcaccagg tttgacatcc aagttaaaat cagtcattag caagttatct
    14221 ggtgtgtagc cgaattgaac attttcaaaa cgaactttgt atggtgtatc tttttcttct
    14281 ggaaggccag atttttcatt ggacatctct tcttcatcca acacttcaaa tacccgttcg
    14341 gctgaagcaa ctgttgattg gatagtgttc atcaaacttg caagttgagt gattggttga
    14401 gagaactgtg ttgtatattg caagaacgct tgaacatccc ctaatgtcat gttcccattt
    14461 gcaactttta ctccgcctag tacagcgacg aacacatagc ctagattttt aacgaaattc
    14521 atcaacggca tgatgattgc cgaaataaat tgagctttcc aaccagcggc atacagattc
    14581 tgattttctt tttcaaatac ttcctggtct ttttcagtat gattgaatgt tttaacaatg
    14641 gtatgaccac catatgtttc ttctacttgg ttattcaata aacctaaact tttttgctga
    14701 tctgcgaaaa acacttgtga tttcggcgct acgatcatca cgatgatcaa gcttaaaggg
    14761 atcatcaata acgcgactaa cgtcaattgc cagctgattg aaagcatcat cacgatgacc
    14821 ccaatcaatg tgactgcact agttacaaat tgagtcaaat tttgctgcag cgtgcttgcg
    14881 atgttatcca tatcattgat tgctcgagac atgatatccc catttgtatg tgtatcataa
    14941 taggaaatcg gtacacgatt cattttggct tccaaatcat gacgtaattc ataaactgtt
    15001 cgttgggaaa cacgtgtcat aataaattgc tgtaaaaagt tgaatacagc agaaatcaaa
    15061 tacatcacga ttactgtcgc aatgatctgc gcgattttat caaagtctat cggaaatttt
    15121 gttacttgtt ggccttgttg catctgttga gcgccagcca ttacaccttt gaatatttct
    15181 gtggttgctt tccccaagac ctttggtgtt tggatttgga agatcacggc tgctattgct
    15241 agtacaaata cagcgatgat cgcaaccata cgtttagaca tatagccaaa caatcggcgt
    15301 accgttcccc agaaattttt aggcttttca ccttttacac cgagatttct gccaggtcca
    15361 ccatgtcctg gaccgcctgc tggtccatga gagggacgtg tgtttgtttt actcatgcta
    15421 agtcctcctc tcttagttgt gaatgaacga tttcttgata agtttcgttt gttgctaata
    15481 gttcttcatg tgttcctttg ccaaccagtt ttccttcatc tagcacaagg atcatatctg
    15541 catccattac tgtacttact cgttgagcaa ctaaaacggt gatactttct ttcatattcg
    15601 ttttcaatgc ttggcgaaga ttggcatccg ttttgaaatc caacgctgag aaggaatcat
    15661 caaacacata gatatctgct gttttgacta atgcccgtgc aattgccaga cgctgtcttt
    15721 gtccaccaga gaagtttcct cctccttgtt ccacatggct atctaagcca tcttccatct
    15781 ctgaaacaaa atctttagct tgtgcgattt ctaatgcttt ccaaatcatt tcatctgttg
    15841 catccggctt accatactgc atattgtctc ggatcgttcc agagaacaga actgcttttt
    15901 gaggaacaaa gcccattaat tctcgcaaat tatattgagt catgtcacta atgtccgttc
    15961 catttatttg gatcgaacca gattcgatgt catataatcg agggatcaaa ttgactaagg
    16021 tcgttttacc tgaaccagtc ccccctatga ttgcgacgat ctcgcccgac ttggcttgga
    16081 aatcaatatc ttccaatgca agtttttctg ctccatgata tcgataattg acatgattga
    16141 aggccaatgt tgcgtttttt ccagcaaaag aaactgtttt aggattttca gggtcatgga
    16201 taccgatttt ttcatccaac acttcattaa tacggtcagc tgaagcttgt gcacgaggca
    16261 ccatgataaa aatagcggaa agcatcataa agctgatcaa gatctgcatc gcataagtca
    16321 taaatgcaat cagattaccg acttccaaag tcatatctgc tatgtagtgt ccaccaaacc
    16381 aagtgattgc tacgtttgta ccgctcatga tcaatgtcat cattggcatc atcaatgcga
    16441 cgatcgtatt aactttgatt gccgtatttg tataatcttt attcgctaaa tcaaaacgat
    16501 tttcttcaaa gcgtgtctta tcgaatgctc gaatcactct gacccctgtc aatccttctc
    16561 gaaataccag gtttaaacgg tcagttttct tttgcatact tttgaataat ggaacagcaa
    16621 agtacatgat tccgccgatc aaaataatca ttactgggat gacaactaag aagatttttg
    16681 tcaactgatg atctttttga taagcaagta tgctcgcgcc aatcaacgtg attggtgcat
    16741 tgatcatcat acgtaaaaac atttgtgtaa ccatttggat ttgagtcaca tcattggtcg
    16801 ttcttgtgat caaagaggcc gttccatatt tatcaaacgc atcttttgtc agactctctg
    16861 attttttata gacatccgta cgcaattgct tacccagttt ctgtgattca cgagtagcaa
    16921 aaaatgtatt tgcgatagca gctaagatac tgatcaaaga aaagccgatc atcacaaaac
    16981 ctgtatgcca aatataatcg acatctcctt gcgctacccc tttatcaata atattggagg
    17041 taagtgtcgg caaatataaa tctgccagta cttggatcac catgaaaact gcagctgcaa
    17101 ttgctgaagt caaggagatc cgttttacga tttttagcat ctagttccct tctttctatt
    17161 tatatactcc gtttttcagc caagaaagtt tcaaattgaa ttcactgatt gcttgctcta
    17221 ctgagtaatc ttcagatgtt gaaaataagc gatacgaatg cgcaattgtt gtaaatacag
    17281 tattcatcac taattggatc agtaattcca attcatgatc attttttact ttcagcttcg
    17341 tccgatcgat caattcaagc aactcttttg catcttgttt gtgttcttta tgtggatcgt
    17401 gaggcgtttt ttggcgactc gtatgaggag cgacttttct tgaggcacgg taatccatgt
    17461 tcataaacaa gttacgataa aaagaagcat tctcgccttc caaaatctca taaatcattt
    17521 tagaaaaata ggattcgaat cctttgaata gatcaccatc ggattctttc attagttgta
    17581 acatatcttt agagctgtca cgtctcattg tctcaaagta ataaaaataa agatcctctt
    17641 tatcttcaaa atactgatag aaactgcctc tagggatatc cgctaatttg acgatattag
    17701 caatcgaagc ttcttttaag ggtactctag aaaactcgat tctcgcagcc tcgattagtc
    17761 ttttttgttt ttctttaggc aaatggaaaa atgtctgtgt cggcatatgg atcctccttt
    17821 ctttcaagat gacacattgt catatgacgc tgtgtcatta taacacctcc tacctgtttt
    17881 gcaagacatt tgcatagttt ttttcattat catccgatac attaaaaaag tgataagagg
    17941 aatccttcca tttgaattaa tgagagttgc ccattaaaat agccacatac taaacaaagg
    18001 atgagataaa tggcccaatc aattagtgtc caagacttca ataaattgcc tctcgatgct
    18061 gacagcatcg ttttagatac aagaaatacc ttagattaca caaaaaacca cttaagccac
    18121 tcgctttcta tccccgctgc tatcttgcca agacaattaa aaaatctaga tccaaagaaa
    18181 ctttattata ttctaagcta tacaggcagg cgatcagaag cattagccga tcaacttact
    18241 gctaatggtt ttcgtgctgt ttatatcatt ggtgggatgg aagctcttca gtcgagtgtt
    18301 gcttgaatat tgcttgttag attcaaaaga ggctgtgaca aaaaatcgtg tcacagcctc
    18361 ttttataccc tgtctttttt tagttattta ttcttcaacc ttatcaatat gacttgatag
    18421 ccttttcttt ttctcatagt tataacaatc atagcaataa aaggagaagg taagcatatg
    18481 gcatctgttt tagtagtcaa aggtcatccc ctaactgcag aagaatcgcg cacagttaag
    18541 gcattgacgt cttttttaac aagttataaa gaaaatcatc cagatgatga agtgactgtt
    18601 ttggaacttt atcgcgatga tattccggaa atcgatgaag aattattatc aggttgggaa
    18661 gcattacgtg ctggtgcaga attcacttcc ttatcagaaa gtcaacaaca aaaaattgct
    18721 cgtttcaatg aattgactga tcagttcctt gctgctgata aaatcgttat cgcaaacgcc
    18781 ttatggaatc taaatatccc aacaaaatta aaagcttggt tcgataccgt caatgtagct
    18841 ggaaaaacgt tccgctatac agaaaacggg ccagaaggat tagtgacagg taaaaaagca
    18901 ctgcacatcc aatctaatgg cggtgtatac aacggacaag actttgcctc tcaatatgta
    18961 aaaggtattt taaactttgt aggcatcgat caagtagatc agctattcat cgaaggaatc
    19021 gattatgacc ctgaccgtgc agatgagttg atgcaaaatg cattagataa agctgctgct
    19081 ttaggtaaat cattttaaaa attgagataa atgcatacaa aagagacaga ggaaaaaatc
    19141 ttcaaagcag catttgtacc ttggatattt tcccatctgt ctctttttgt tattctttct
    19201 gctttttcaa cggattttct tcataaagag aagagattgt tgtgtaccat tcaaaaacat
    19261 gagtaatcac tactttagct gctgcataaa caggaatccc caagatcaca ccgatgattc
    19321 cgaacaattt cccagaagtc agcagaacta ataaaatcgt tactggatgg attgctaact
    19381 gattccctag tactaatgga gagatcaagc gtccttctat tgtctgctct actgcaaaaa
    19441 cgatcagtac tttgatgatc atgaatggac ctgctacaat ggctaaaaag atagccggaa
    19501 tcatcgctaa aaatgatcct aagtaaggga tcaggttcaa aaaacccgca ataatcccca
    19561 acgtgacagc atactctaaa ccaatgaccg caaaaccaat cataaacatg attgccacag
    19621 caaaagccac agttagttgt cctcttatat aagaagaaac ttgactattc atttcactca
    19681 gcacattcaa cgtcggtttt tgcatgcgtg tcggtaaaaa tttgacaaaa taaggtgcta
    19741 attgtttacc gtctttcaat agataaaata agataaacgg catcgtcagc agagccacta
    19801 aaatagtcgc taccgcgccg acaaaacttc caagactctg aactgtactc ttagatatat
    19861 cttggatcat atcgcctgcc gaattcatga acttatcacc tgctgtttcc aactgttcgc
    19921 gaaattggtt gaacagcggg tcccgcaaaa tctcggttaa tttattatca atcgtatcta
    19981 catattgagg gaagttactg atgaagctca ctgtttgttc ctgaatctta gggatgatca
    20041 ctacgctccc ccatgcgatt agtgcaacaa ccaaaacaaa cagcccaatg atactgtaaa
    20101 gccttggtac ttttcgcttt tcaaagtaat caactaccgg attcatcaag taatacaaga
    20161 ttcctgctaa aatgataggc agacctacga ttgctaaaaa ttgccagatt ggtgcaaaca
    20221 aatatgaaac ttttgtaaat aaaaagacga tcagcaatat taacaataca acaagtaagg
    20281 ctgtgactac ttgattattt aaaaaccagc gccaaaacca cgataccggt ctgttcttct
    20341 tattttttcc ttccataatt cctcctgtta gttataaaaa atttggcttc ccaaaccaat
    20401 agcaggcagt ctatgcggat gaagatagat tccatttgca atttgtcctt cttctggaca
    20461 agcatcaaaa atcaatgata cgtgagctac tgtctctaaa ttcccattgg ccatcggacc
    20521 gacataatcg atcgtatata cctgatcatc aaaagcaatt gttccgcctg ctttcaaaga
    20581 aaaaggttcg tcttttgata cttcttgaat caccgaatat tttcgtaagg cagctgttgc
    20641 tgttttatcg aacaaaatca gcatgggttc ttccttgttt attgcttctg tcccaatttc
    20701 tgtaatcttt gcctgaatca ttgcactctc tcctttaatt gctttcaaaa acagtataac
    20761 acattctcat tctttagccg tgtggaataa ttatgtgata gaataaaaac gaaaactatt
    20821 tcattacaat taggaggaat cagctcatat gatcgaaaag ataattttag gaacctatac
    20881 gcgtcgcgaa agtaaaggaa tctatacgat cgaccttgat acagaaaaag aagaattagc
    20941 caacctttca ttagctgttg aagaaaatag ccctacctat gtagcaaaaa gtaaagctgg
    21001 caatctttac acagttacta gtgtggatgg attaggtggt gccggtgcgt acgataagga
    21061 ttttcatttt ttgaatgcgg taacagagtc tggcgctcct ttgtgttatg tagcggttga
    21121 tgaagcacgt caattactat acggggcgaa ctatcataaa ggtgaagtaa atgtctacca
    21181 tatattagga gatggcgaac tgaaagcagc agattcaata ttccatcaag aagcgacagg
    21241 tcctcataaa aaccaagatc atgcgcatgt ccactatacg gatctgactc ctgaccaacg
    21301 attagtcgta tgtgatcttg gtacagatcg tgtgtatact tatgatgtgt ccgaaaacgg
    21361 caaattgaat cttgtaacta ccttcactgc agagccagga acaggccctc gccatcttgt
    21421 ctttcatcca aatggacaat tcgcttattt atttggtgaa ttagacagca cagtccgcgt
    21481 gctttcttat aataaagaaa caggtgcttt tgaagaatta cagaaaatat cgaccttgcc
    21541 agaagaattc gacggtgaaa atggaggggc agctatccgc atttcaaatg atggcaagtt
    21601 cctttatgct tctaaccgag gacataactc gattgctgta ttcgcagttt ttgaagatgg
    21661 ttcccacatt caaaacaagc aaatcatctc aacagaaggg gattttcccc gcgatttcgc
    21721 actagatcca agcaataatt ttgtcgtgtg tgccaatcag aacaccgata atctgacatt
    21781 atatcgccgt catcctgaaa caggattact ggaattactg aaaaaagatg tctatgtacc
    21841 agaatgtgtc tgtgtttatt ttgatcgtta gtaagaaaag gagaatgaaa tgaatcagca
    21901 agacgtatta gacagactac gggaagaact aaatattcct ttctttgatg ggaaaatcga
    21961 agataaagag tatagcgaag aggaatatca gaaactcaaa gccgatttac agaactactt
    22021 tgaacaatat gtacagaatg tagaaaacta atcagctttc catactatct gaaaagaaca
    22081 tcaggacaag acttttccgt ctatcctgat gttcttttct acaaataaaa aaccgccgag
    22141 atggttagac tcgacggaaa aggagtattt actcgatttt gagtaagtac aaggttgttg
    22201 ttggttatag gtatgtcatt atcataaagg attttgatca ctctatcata aaggtaatat
    22261 tacatttata ttagataact ctagttttta ttgttatcaa agatttcttt tattttttca
    22321 acaacaaatt agtcattttt tggatggcag ctgtcaattg gtctgtcgct tggtcttgtt
    22381 ttgcttcaga tgtattgaat agatcttcat ctacccctga ttcaagatcg atactttgga
    22441 tcatttcttc acagcctgct acatatgaaa gataagcttt ttcaaaattt ttatgaatgc
    22501 ccattgcttt tgctggtgga cgcaaagtac tgactttctt aagcattgcc cgatattttt
    22561 ttgttccttc ttcaaataac aggatgatct cttcaagagt cgatttgtcc agttcacttg
    22621 tttttccttc atcaattgcg tttctgatca cttcatattt cggatgcatt ccttctccga
    22681 ttgcttctgt ttccttgacg atttgatcga tggactgcac ataatagcca atattcggta
    22741 acatgttcgt tttcctactt tctccattgt tgttattatt tattctactt gtttttatcc
    22801 tcaaatgaca agtacttgaa aaaatttaat ggtttctata cagctcgcca ttcttattcg
    22861 tgctcaaaga aaccatctgc taaactatat tgtttccctg cgacaatgtc atattggatc
    22921 aattgatatt cgtcgtaaat ctcttgttgc gagcgattga attgcgcttc tttataccac
    22981 tgtccgtttt gatagtacag ttctcgttca aacgccggaa gttcctgttc taatgataaa
    23041 agcagctgat aaaagccatt tgttgtctga ttcgtctgtt ccattagatt cgctgcaaaa
    23101 taaaacggac tggtgattgc atcgtgagtc gtttgctttt ctaattctcc tttactgtcg
    23161 aacataagaa attgtgtttc atgcaatgtt tgtttgtcat tttttgcttg gatcgtatct
    23221 gaatagatcc ctggaagatg atccccccaa aaaacaacta aagtccttct agaaagattc
    23281 ttcaattctt ccgtgaactg cttcagtgcc gtgctgctat aggaaatatc ttgcagataa
    23341 ttctctaagt caagtgtccc acttccatct gaagtttttg caggatagtc tagattatca
    23401 tactttccat cgtagggcat atgtgtctgc atcgtgacta aatgaatgaa ctgaggtgtt
    23461 ttttcttcct tgagaagtgt cattacttct ttgtaagctg actcatcaga aatatatgga
    23521 ttattctcta tcgtatccgt ataggtcatc gtacgctcgc tgatgaactg gtcaaatcct
    23581 aatgtctgat aaacatcctc tcttttatac atcgaagtat tgtatggatg gatcgctgtc
    23641 gtctggtatg attgtgcgtt taacgctgaa acgatagaag gaagctgatc catttttggt
    23701 acaagcatcg tgtaaggggt ggtgagttgc gcattaaata acgccataga aaaaccagtc
    23761 aatgcttcaa attcgatatt tgctgttcca ccgccatagt tttgagaaag catattgcca
    23821 ctatatgttt gatcagctac ttcataataa tctgccaatg gctcaccaga tacttctacg
    23881 ccgtttaatc gactaggatc tgagaaactt tcactcatca cgaacacgat gttcggcgac
    23941 tcttcctcta cagttttatt tttttcatcg gctagcttct ggtatttttc agtgatttct
    24001 ttgatctttt ctttcgaata tccttcaggt tcatccattg gttctacctt cagattgtac
    24061 aaaaacccac cgataaaacc agtgttatag tagttcattt tctggctgta aggtatccat
    24121 agtgccgttt tattatatgc ttttcgcaac aaattatcgg gattgttgaa attactgata
    24181 taccctagca gtccaattgc cacaagcagt gtcgttaatc ggatcaattg gaatcgcctt
    24241 ccttttttca ggcttcgaaa catgtaccag caaaacaatc ctatcacggt tcctgcggca
    24301 gctaagatca ctgtaaacag catagtgccg gtcatatctt tcagcaatcc gatttcagtg
    24361 atcatcttca aatcgtctgg ataaatcggt tcttgccgat aactcatttt catataattt
    24421 gcaaaaccta atacaccgat ggctacgaca tacgtaaggg aacctaaagg gacagatcct
    24481 gctagtgcaa tcagaaaaat aagaataatc agcaagacga gacaggctaa gaagaatttt
    24541 tccgtatgcc aagaaaaagc aaatttcaac gcaaggtcta cggatagatc attttgacac
    24601 cactgcaaaa ataaattact aaaaacaaca agaaaaaaga tactgatgat tgctgctgtt
    24661 ccttttaaaa tctttttctg tttggtcgaa agtctgctta tatatctttt caaatgcttg
    24721 ctccttcatt tctatcaata aaagaatgtt gcttctctta ttttagttgt acgaaattcg
    24781 aaagtaaaga acggaaagaa gaattaatga aatccaaata ttctctttct ttatggcctt
    24841 tctggtttct ttaataagcg gacctttcgt actccagaaa ttttttctag ctgttgtttc
    24901 acttgattga gctgttcaaa atcagtactg tctatgtcaa tcaatatgta gacatgctca
    24961 ttcttatggt tacgcgatat atctgcgatc gctagctcgt tctcattcag cagagaaaat
    25021 atctctgcta atatattggt tttcttttga taaaatatag tgaatcgaaa cggcgcttgg
    25081 aaaaacattc gtgcggatgg ataattgacc gcttctctaa ccgtaccgaa caaaagataa
    25141 tcttttaagg cttcaactgc caaacgtccg ctgtctgcca aagcttcctg agtcgatccc
    25201 cctagatgag gcaacataag tatacggtca ttttctaaga actcttcttc tggaaaatca
    25261 gtcaagtatt tagccagatg ttgtttttcc aatgcttgca taacagcata tttatcaaca
    25321 atttcacttc ttcctacatt gataagtaca gcatcttttt tcattttttc tatattcgcc
    25381 tgatcgatca atccttttgt atcttctgtc aacggcagca tgactacgat gtaatccgag
    25441 gtagaaagaa ctgtttctaa atctgcttgc cgaacataat ccaactgggc atcgcggata
    25501 gaataaccca agacatccat ccctaagcta tagcaactta gcgctacttt tgtgcctatc
    25561 gctccaagac caagaagacc gatggttttg ccttgcaatt ctcgtcccac ataagcactt
    25621 cttttgtttt ctgcctgttc taaaatatta ggacctgtta gtgtttgtac catacgactt
    25681 gcttcgatta tcggacggga agataagagc aaacagcaca aaacaagctc ttttactgca
    25741 ttagcattta cacctggtgt attcatgacg atcgtcccgt tttcagaagc tttttcaacg
    25801 ttgatcgtgt tcaccccaac accagctctt gaaattgcta gcaaatctgg tgtaagccat
    25861 tcatctttca ctttcgtact acgaataact attccttcgg ctggcaaatc atctattatt
    25921 ttgaaatatt ccccttgtaa cttttctatc tcttgttctg gaatagcatc aatcaaatga
    25981 acgtgataca taaaaccccc tccttactaa caagtatctc gaaatttctg caattagaca
    26041 actgtttacc atttgacttc ctagaagcaa aaagagcagt tagagcagag taggctgagt
    26101 gataaaaata aggctatgac aagttactgc cataagcctt attttcgaga gcaaaatatt
    26161 gagggtgaga tatcttgata aatggtgttc cgttagctga cattgataat aagactaaat
    26221 ttcaaaaaag ttgaaaagca aatttcgtca atctcttctt atttcttaaa actaagtggc
    26281 tttttcacaa cctcttttta tctaataacc actgtttcca actcaactaa ttttgcccac
    26341 gtttcaattt gatctgttgt tagatttagc gagacaacgg tatgatgacc gccacctgcc
    26401 tgaatccatg aatggacacc ttcatggaaa ttcggtttag ttttccataa cacacgtgca
    26461 acaggtaaat taggtgcttc agtcgttggt tcaaaggctt ctacttcatt gatcagcagt
    26521 ttataatgcg taccgaaatc tgccatcgat acgactaccc cttctcctgc tttcccatca
    26581 aatacaagac gcgccggatc ttctctatct cccattgata aaggagaaac aacgattctt
    26641 ggtttcgtcg cagcaagcgt cggatctact tccatcatat gggattccaa gattgcttct
    26701 tggccactag ctagctcgta agtataatct tccatgaatc ctgtatcttt ccctcgagcc
    26761 atgattttca gcaaccgatc aatcgctgca gttttccagt cgccttcccc agcaaaaccg
    26821 tatccttctg ccatcaaacg ttgtacagct agtccaggta gttgtttcat tccatacagg
    26881 tcttcaaaat tagtcgtaaa agcgttatag cctccttctt ctaagaaacg gcgaataccg
    26941 atttcctgct gagcttgtac ttttacatgt tcctcccatg tcttttcttc ataatcacca
    27001 tagtcgaagg tatacaattc cttgtactct tcaaataatt gatcgacttc ttcgtctgat
    27061 acgcgatcga ttacttgtac aagatctccg ataccaaaat aatcaacagt ccaaccaaat
    27121 tgaatctgtg cttccacttt gtctccttct gtgacaccca cgtttctcat gttgtcacca
    27181 aaacgagcca cttttattcc aaaactttca ttataggcta ctgcaacatc catccaatca
    27241 gcaatctctt tttggatctc aggtcgtttc cagtagccaa cgacgatttt attttgttta
    27301 tttaatcgag cattgatgaa cccatattcg cggtcaccat gagcggattg atttaaattc
    27361 atgaaatcca tatcgatcgt tttccaagga atgctttcgt tatattgcgt agctaaatga
    27421 agcaatggtt tttgtagaag ctttgtcccg cggatccaca tttttgctgg tgagaaagta
    27481 tgcatccaag tgatcacacc tgctacttct tctcgataat tgacctcttt catgattttt
    27541 gtgatcgtat cagcagtaac tgctaattct tgaaggcgga ttggataagg caattggcca
    27601 ttttcattca gttcatcgac catttcttgt gcatgtttct ttacttctcg cagtgcttct
    27661 tccccatata aatgctgtga tcctacaaca aaccaaaatt ctttttctcc aatattcaac
    27721 ataattagcc actcctttta tttgttttgt ccgtaatacg cattttttcc gtgttttcgt
    27781 aaataatgtt tatctaaaat ccgttgaggc agctcttctg aaaatgaatt gagctgtctg
    27841 gtaaataaat tcatcttgca tacttcatct agtacgactg cgttcattac cgcactttgc
    27901 gcatctttcc cccaagtaaa tggaccgtgt ccgtgtaaaa gtacccctgg aattgctaag
    27961 ggatcaattt tgcgttcttt gaatgtttct atgataactt ttcctgtttc atattcgtac
    28021 ccgctgtcga tttcctgttg tgtcaaaaat cgagcacaag gtaccgatcc ataaaaagta
    28081 tctgcatgcg tcgtccccat cgcaggaaca tccaatcctg cttgtgccca gatcgtcgcc
    28141 catgtcgagt gagtatggac gattcctccg atttgaggaa atgaacgata taaaacagca
    28201 tgagtaggcg tatccgaaga gggattgagt ttaccttcaa tgatatgatt gtccaagtcc
    28261 acaacgacca tgtcgtctgc tgtcatctct tcataactga ctccgctagg tttgataacg
    28321 aaatatcgtg tttcaggatc aaaagcgctc acatttcccc atgtatattt gacgagtccg
    28381 tgtttaggca agtcaagatt tgcttggtat acttcttctt tcagttgttc cagcattttc
    28441 tatcactcct ttttcttgca attgatgaat cgctgttttt tcaatgtcga tcccctcacg
    28501 gtaacgtctc ataaatatct gatagcctgc tatttcttct gctgaaggtg tgatggtgat
    28561 tccttggtct tgtccaaaca cgtcttctgc caagaagttt tctaaagact tcccatgtcg
    28621 atccatcata taggctgcta ataacgcgat tccccacgct ccaccttctc cggctgtgtc
    28681 catcactgta actggtgcct ccatagcaga tgctagaatc cgctgtccaa cttctggtgt
    28741 tttaaaaatc cccccatgcc ccactaaacg atcaatttgg acattctcag atttcaatat
    28801 atccattccg attctcattg caccaaaagc actggctaaa tggatccgca tgaaattcgc
    28861 taaatcaaaa cggctacctg gtgtacgtac aaataatggc cttccttcat ttactcctgt
    28921 aatgttttca ccggaaaaat aaccataaga aagtaatccc ccgcaatccg tatcgccttc
    28981 tagcgctttg ttgaataata ccgtgaataa tttatctctg cttatctcca tacccaaaga
    29041 attggtaaat tcttcaaaaa tactgaccca agcattgata tccgaagagc agttattcgt
    29101 atgaaccatg cctactagtt ctcctgaagg tgtagtgacc atgtcgattt ccggatggac
    29161 attttctagt tctttttcta atacgatcat agcgaaggcg gaagtaccag ccgaaatgtt
    29221 ccctgtccgt ttggctacac tgttcgtagc gaccataccg gttcctgcat ccccttctgg
    29281 cggacaaacc gggattcctg gtctcaaatt tcccgtaggg tcgatcagat aagcccctga
    29341 ttcgcttaac ttgccagctt cttctcctgc tactagaact ttaggcaaaa tgtgatccaa
    29401 gtcaatcgct acgtgcgctt tttcgattaa ctgaccaaaa atatttttca tttcttgatg
    29461 gtaggttttc gttgaaggat cgataggaaa catgcctgat gcatctccta cgcctagtac
    29521 tttctcacca gtaagctgcc aatggacata acctgcaagc gttgtcaaaa aattaatttc
    29581 tttcacatgt tcttctttat tcaaaataga ttgatacaaa tgagcgatac tccatctctg
    29641 cggaatattg tattgaaatt tttccgttaa tattcgttct gcttcttcag tgattgtatt
    29701 tcgccaagta cgaaatggta ctagtaactg ttccttgcta tcaaatgcta gataaccgtg
    29761 catcattgca ctgaacccaa tcgacccgat tgtcgtaaga ttctcgccat agatttcgaa
    29821 tacttctgct gccatttttc ggtaactgac gcgcagtcct ttccaaatat cttctaatga
    29881 ataggtccag ataccatttt ctagtttgtt ttcccactca taactaccag cggcaatagg
    29941 agtaaaagat gaatcaatca atacagcttt gatcctagtt gatccaaact caatccctaa
    30001 agatgtacga ccatctatga catctaacat actttgcgca tttacttcct tcataatgat
    30061 ttaactccct ccttctcgtc aaacctgatc aaaccgtctt tttagtagcg cttacaatga
    30121 ttattgtata cttttgtacg tacatgtcaa gcggttattt acaagaatac aataaaaaac
    30181 acttgttttg tacgtacata agttataaaa tgaaattgct tacaaaatct gatcaaccgc
    30241 cttctcgaaa ttctagaagg agagaatact tatgaaaaaa gaaaaaaaag tttcctcatc
    30301 gtttatttat ttttttggcg catttggcgg cattctattt ggatatgata ttggtgttat
    30361 gaccggcgcc ttaccttttt tacagcatga ttggggtttg gcaggtaaag cttctttgat
    30421 aggatggatc acgtcttcgg tcatgttagg tgcgatcctt ggcggctctt tatctggtct
    30481 tctttctgat aagcttggac gacgaaaaat gattcttctt tctgcactta tttttatggc
    30541 tggttctgtc ctttctgcat ctgcccctca caatggttct tattttctga ttgcagcaag
    30601 gattcttcta ggtttagctg ttggcgctgc atctgcccta gttcctgctt atatgtctga
    30661 aatggctcct gctagactaa ggggaagatt gtcagggatc aatcaagtga tgattgcttc
    30721 tggcatgctc ctttcctatg ttgcagatta tttgctaaaa gtgttgcctg aaacgatggc
    30781 ttggcgcgtg atgttagggc tcgctgctgt ccccgcgctg attttatttt tcggtgtttt
    30841 agctctacca gagtcaccgc gttttcttat gcaatctggc cgcttggaag aggcaaaaag
    30901 agtattgaac tatattcgga caccaaaaga agctgaacag gaattcgaac aaatccagct
    30961 gaatgtcaaa caagaaaaga caaccggaac ttcttggcat actttgtttt tagaaaaata
    31021 tcgttcctta gtcattgccg gtattggtgt agcagtattc cagcaatttc aaggagctaa
    31081 tgctattttt tactatattc cgctgatcgt agaaaaagcg actgggcatg cagcgagcga
    31141 tgcattgatg tggccgatca tccaaggaat cattttagtt gctggttctt tgctcttttt
    31201 ggttattgca gacaagttca atcggcgaac tttattgaaa ataggcggta gcgtgatggg
    31261 actttcattc atcctaccag ctgttttagg aactgtactg gatgctcata caaattcgtt
    31321 gttgattctc ttgtttttat gtatttatgt cgcattttat tcttgcacat gggctccact
    31381 gacatgggta atcataggcg aaatattccc actagctgtc cgcgggcgag catctggcct
    31441 agcctcttct tttaattgga ttggttcttt cctagttggc ttgctttttc ctgtgatgac
    31501 tgcttctatg tctcaagaaa tcgttttcgg catttttggg atcatctgct ttttaggggt
    31561 tttatttatt caagagatcg taccagaaac tcgtgggaaa tctttagagg aaatcgaaca
    31621 aagtgcatct aaaaaaactt atcctaaacg tatttctatc catcattctt aaagaggttg
    31681 tgacagaagt ggtcagcttc aagaaataag aaggaaccgc ttagtttcca gtcacaagga
    31741 acaatttcaa aaaacagctt ctcatgtttg atgaatattg tgacttgtga ccagttggta
    31801 ggagaacacc gtttagtgga gaggtggtga aagaaacgtt cagccttgag cgatgacaaa
    31861 agctctcaca aacattgcga cgagtaaccg taggagcaat aagaggcggt aaaaacccgt
    31921 ctcttatttt tcgttttctt tcactgtttc ccgttcaact agtacaggtt catagagaat
    31981 atttggctgt gcctgtcctt tttcaatcgt tgtgacgatc caccgtgcgg catcttgtcc
    32041 caacttttct tttggatgcg tcaatgttgt caatttttta ttccctaaat ggcttaaaga
    32101 agaatcgtct gttcctacga tcgataggtc atctggaata tgatagccat tttctgtcaa
    32161 cttatctagc attaaactag ctatttgatc gttatagcaa atgatgccat ctaaagcttg
    32221 ttcttctttc aaccgttgta ctgttttttc agcgattgtt tctcgtgttt ccgtggtgaa
    32281 agtaatgata tcttccgtca aaaaacgcac attatactgt tcacacgcgc tgataaaacc
    32341 tttcatcctg tattttcctt gaagatcgtc gatttttgtg atgaacaaca gacgctgatg
    32401 tccttttttt atcaaatatt ctgctgccat aaaaccgact ttcacatcat ctaaacaaat
    32461 ataagaggca tttaactctt cataagcagc attgatcatc accatcggaa tgccttgttc
    32521 tctcaaagca acataaagtg ctaagtttgg gttatactga ttacttttcg taggctcaac
    32581 gatcaaacca tctaccccat aagcaatcat tttcttcaaa cactctttct ccaattcgtg
    32641 atcgttattc gtactggcta aaagcaatga atacccagat tctcgtaatt cattttcgat
    32701 tcctcggata atcgacggaa aaatataatc agagagatat gtggtgatga caccgatcgt
    32761 ttttgttctg ttcaatggtg ttttttcttt cttatagctg tcatctacat atgtgcccga
    32821 ccctttttct ttacgcagat agccctcatt taccaacaca gcgattgctt gccgaatcgt
    32881 atgtctgctg acgtcatata tttcttgcag cttgaattct gagggaatgg ttgtatttac
    32941 ttcgtattcc tcacgaagga tcttttgttt tatctcttct gcaatatctt gatattttgc
    33001 catttgcctt gccccttttt atttgtacgt acatatatca taacgagtct gcaaggttta
    33061 ttcaagcaaa aatcaaggtt tcttgtgctc ataaggcata cggaacaggt tcaccaaatt
    33121 ctggaaatcc ttgctcattc cacgtaaagc attgcgcgtt cgcgtgccga ttaggattgt
    33181 ctaacggatc ccctttttgg ttcttctctg gtcgagcatg atagatcaaa atatcaaagc
    33241 acccgtcttc tgaaaccgta aacgagttgt gaccaggacc aaattgctta tttttttcag
    33301 aagaaacgaa tactggctcc gataatttat gccatgattc ttttaataaa agattcttgt
    33361 gctcatcagc ccacagcagg cccatggcat agttttcatc tgttgcacta ccagaatacg
    33421 taatgaatat tttcccattg cgagtaagga ctgctggacc ttcatttact gaaaaacccc
    33481 gtttttccca ttcatactca ggtatagaaa ggaggatctg cttccccttt aacgtccaag
    33541 gattatccat ttcggataga taaagattag aattcccagt aatttcagga tcttgttggg
    33601 cccacacata atatagcttt ccttgagaag agaaagttgt tccgtctaaa ctgaaagttt
    33661 cccttttcgt ttttatttgt cccttttcta tccaatctgt attcattgga tctatatgct
    33721 gattttcaag tacgaacatg cggtgatgat gagattcatc acgtatagtt ttatgattgc
    33781 ttgcagcaaa gtaaatatac cattttccat tgatataatg gacttcaggt gcccagatca
    33841 attcactcat cggtccttct tcatgagcct gccatacaat caggcttgca gcatgttcta
    33901 attcattcag tgactttgcc cttcttaatt caatcacttg gtagcctgga accgatcctg
    33961 taaaataata ataaccatct gtatgtttat atacccaagg atctgcccgt tgccgaacga
    34021 taggattatt ataaagtgtc ttcatcataa caaatttcct cacattttct ttattctaat
    34081 tacattccaa gagtattttt ctatccctac atttaattcg tcatgttttt ctattgaaaa
    34141 atagtttatt tcaggtttta ttttttctgg ttcttcacgt gtatttttgt ccaacagatc
    34201 gctactggtt aatgtgtagt gctctccttg atctagcaaa ttgaaatcat gttcaatcgt
    34261 tatcttttgt tgttcatttg tgcgattgat caagaaaata gtcagctgat tgccgttatc
    34321 tacgatcaca ctgtccacat aaggaacatt ttttatcttt tcacaatcat aaacagggca
    34381 aatgatttgt tgtttcaata cttgaccttt agcatatttc gataagtgtt gcatcacata
    34441 ataagttgga ttcctccatg cttgtccttt agcatctgtc agtattaaag gtattgtgtt
    34501 tactaacaat gattgacaac taatctttac gcgatctgcg tgtcgtaaaa tagccagccc
    34561 taatgaacca aataataatg tatcttccat agtaaagtaa caccaatcga tgggactacc
    34621 gacttcaaaa tccctatgtc ttttttccgg ttgggcatgt acattccatt catcaaacgc
    34681 tagatacatt gttttctcac ttctcaaata tccttttaca taatcacaag tagcaacgat
    34741 ttcttcaatt tgacgatcga atcgataagc ttttgcaagg tatgtcgctg tatcatcacg
    34801 ctcattttta ggaggtttag tcaagtcttc ttcatcatac ttatcaatat agttatgcaa
    34861 agctaaataa tctacattct cataagcttc ttctaatgcg atacgatccc attcagggta
    34921 cgatggtaaa cgtggcgtag agcttccaac taagattgtt tcaattgaat catccactaa
    34981 cttcatgact tttgaggctt catttgctaa cctaccatat tcataagcac tcttagcccc
    35041 cacttgccat ggaccgtcta gctcattccc taaacaccat aatttaacgc caaaaggctt
    35101 tttttgccca ttttttattc ttaaatcact ccagaaggtt ccttttggaa aattacaata
    35161 ttctactaaa ttttttgctt catcaattcc ttttgttcct aaattcaccg tgaatatcgg
    35221 tttcgcttgt acatgactga tccatttaaa aaattcgtgg agtccaaaag aatttggttc
    35281 tatacctcgc catgctaaat caattttttt tggtcgttgt tctttcctac ctacagtatc
    35341 cacccaatca taaccagatg taaagtttcc tccaggatag cgaatgacac ctaattctaa
    35401 ttcctttaca taagagagga catcttgtct aaacccgtca ccatcagctg tttcatggcc
    35461 cggttcataa atccctgtat agacgacact tcccatatgc tcaacaaatg agccaaacag
    35521 ccgagaactg atctctccta ccgtaaaatc ctctctgatt tttactttca cttcttcgtc
    35581 caacagcttt ccctaccttt catttcctat agtcaagata atagatacaa gtttccatac
    35641 gccaccagca aagataaaaa taaaaggagg gaaaagaaat atgagcagta caaatcctaa
    35701 taacaatacg caacaaagaa tatttctcaa aaaatcaaaa aatactttcc atgcactttg
    35761 ttcaaaaatt tgtaaaaatg tgatatcttt gttttcagaa atcacatata tccaggataa
    35821 aagaaacatg gaataaataa gtaaaactac atatccacta attgaaagta cgattgtctg
    35881 ttcttctaat aattgattaa caagaaaata aataatgatc gttaaca
//
LOCUS       DACDIE010000025        35678 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.025, whole genome shotgun sequence.
ACCESSION   DACDIE010000025 DACDIE010000000
VERSION     DACDIE010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35678
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.025"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            356..631
                     /locus_tag="IV250_001326"
     CDS             356..631
                     /locus_tag="IV250_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8768620.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            644..2722
                     /locus_tag="IV250_001327"
     CDS             644..2722
                     /locus_tag="IV250_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP8768621.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            2882..4768
                     /locus_tag="IV250_001328"
     CDS             2882..4768
                     /locus_tag="IV250_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP8768622.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4780..5727
                     /locus_tag="IV250_001329"
     CDS             4780..5727
                     /locus_tag="IV250_001329"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP8768623.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5747..6274
                     /locus_tag="IV250_001330"
     CDS             5747..6274
                     /locus_tag="IV250_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP8768624.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6337..6990)
                     /locus_tag="IV250_001331"
     CDS             complement(6337..6990)
                     /locus_tag="IV250_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP8768625.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7122..7964
                     /locus_tag="IV250_001332"
     CDS             7122..7964
                     /locus_tag="IV250_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP8768626.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8122..9018
                     /locus_tag="IV250_001333"
     CDS             8122..9018
                     /locus_tag="IV250_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP8768627.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            9021..9734
                     /locus_tag="IV250_001334"
     CDS             9021..9734
                     /locus_tag="IV250_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAP8768628.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9754..10272
                     /gene="msrA"
                     /locus_tag="IV250_001335"
     CDS             9754..10272
                     /gene="msrA"
                     /locus_tag="IV250_001335"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP8768629.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQDYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10269..10496
                     /locus_tag="IV250_001336"
     CDS             10269..10496
                     /locus_tag="IV250_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAP8768630.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10648..11199
                     /gene="lepB"
                     /locus_tag="IV250_001337"
     CDS             10648..11199
                     /gene="lepB"
                     /locus_tag="IV250_001337"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP8768631.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11282..11824)
                     /locus_tag="IV250_001338"
     CDS             complement(11282..11824)
                     /locus_tag="IV250_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP8768632.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(12061..12459)
                     /locus_tag="IV250_001339"
     CDS             complement(12061..12459)
                     /locus_tag="IV250_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP8768633.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            12719..13579
                     /gene="ylqF"
                     /locus_tag="IV250_001340"
     CDS             12719..13579
                     /gene="ylqF"
                     /locus_tag="IV250_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAP8768634.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            13572..14339
                     /locus_tag="IV250_001341"
     CDS             13572..14339
                     /locus_tag="IV250_001341"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAP8768635.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            14395..15252
                     /gene="dprA"
                     /locus_tag="IV250_001342"
     CDS             14395..15252
                     /gene="dprA"
                     /locus_tag="IV250_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAP8768636.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            15374..17452
                     /gene="topA"
                     /locus_tag="IV250_001343"
     CDS             15374..17452
                     /gene="topA"
                     /locus_tag="IV250_001343"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAP8768637.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            17421..18797
                     /gene="trmFO"
                     /locus_tag="IV250_001344"
     CDS             17421..18797
                     /gene="trmFO"
                     /locus_tag="IV250_001344"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAP8768638.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            18995..19900
                     /gene="xerC"
                     /locus_tag="IV250_001345"
     CDS             18995..19900
                     /gene="xerC"
                     /locus_tag="IV250_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAP8768639.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            19937..20485
                     /gene="hslV"
                     /locus_tag="IV250_001346"
     CDS             19937..20485
                     /gene="hslV"
                     /locus_tag="IV250_001346"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAP8768640.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            20499..21899
                     /gene="hslU"
                     /locus_tag="IV250_001347"
     CDS             20499..21899
                     /gene="hslU"
                     /locus_tag="IV250_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAP8768641.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            21917..22708
                     /gene="codY"
                     /locus_tag="IV250_001348"
     CDS             21917..22708
                     /gene="codY"
                     /locus_tag="IV250_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAP8768642.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            22790..23665
                     /locus_tag="IV250_001349"
     CDS             22790..23665
                     /locus_tag="IV250_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAP8768643.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(23797..24435)
                     /gene="plsY"
                     /locus_tag="IV250_001350"
     CDS             complement(23797..24435)
                     /gene="plsY"
                     /locus_tag="IV250_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAP8768644.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            24562..24996
                     /locus_tag="IV250_001351"
     CDS             24562..24996
                     /locus_tag="IV250_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAP8768645.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            25162..27207
                     /gene="parE"
                     /locus_tag="IV250_001352"
     CDS             25162..27207
                     /gene="parE"
                     /locus_tag="IV250_001352"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAP8768646.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            27219..29669
                     /gene="parC"
                     /locus_tag="IV250_001353"
     CDS             27219..29669
                     /gene="parC"
                     /locus_tag="IV250_001353"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAP8768647.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDRSIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            29946..32177
                     /gene="pflB"
                     /locus_tag="IV250_001354"
     CDS             29946..32177
                     /gene="pflB"
                     /locus_tag="IV250_001354"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAP8768648.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            32421..33182
                     /gene="pflA"
                     /locus_tag="IV250_001355"
     CDS             32421..33182
                     /gene="pflA"
                     /locus_tag="IV250_001355"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAP8768649.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            33267..34193
                     /locus_tag="IV250_001356"
     CDS             33267..34193
                     /locus_tag="IV250_001356"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAP8768650.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            34375..35268
                     /locus_tag="IV250_001357"
     CDS             34375..35268
                     /locus_tag="IV250_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAP8768651.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            35402..35527
                     /locus_tag="IV250_001358"
     CDS             35402..35527
                     /locus_tag="IV250_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8768652.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
BASE COUNT        12468 a         6131 c         7511 g         9568 t
ORIGIN      
        1 ttaatcatac tagagaacga tatgtttttt aatacctaaa ataaaaatgg ggctgaagaa
       61 tcaattctga ttcatcagtc ccataaatta tagagccgat ttttgtcgca gccccatttt
      121 tttgttgtga taaaagaaca agaaaggcag gaatgggaaa gtaaacatcc ttttgtcaga
      181 aacttttatc tttcttaagt tttacttgat atctgatact atttggaata gaatgaggcg
      241 ttgaaaaatt tatggaaatt tctaactagc aataaacgat gaaacaatga aatatttttc
      301 atccaaaaag aactttgcta taatgatgga agtttgtgta taggagatct ggaaaatgag
      361 agagagtttt gaacaacaaa aaaaactgtt gcatgatcgc tatggcgccc tttcgatgga
      421 tgaccgccgt caaatacttt gtaagttgcg taaaagaaat atattgatgt atcgtcagtt
      481 ggaacgtctg aaacatgatc tgcttcgttt ggagtcaaaa cgtgtacagt gtgaattaga
      541 ggggaaccaa acacaggttg aagtagtcga aaccaaaatt ttaaagaaaa aagaacaatt
      601 tttaaaaatg cttacacaaa ataaaaaata aaggggatgg acgatggaat ttgctgagtt
      661 agttattgta tttgcagtaa cgatcacctt atcgaacata gctagccgca ttttgccaat
      721 gattccagct ccactgatcc aaattttcct tggagtggta ttaggtctga cagagtgggg
      781 acaatcaatc gattttgaac ctgaattgtt cttagttatg attattgcac cattgttatt
      841 tagagaagga gaaaaagcag atatttcttc gattctcaaa aatttcggga caattttgtt
      901 cttggcattt ggaggagtca ttttgaccct tgtcggggta ggtgcgacgt tatcattcct
      961 gttgccaagt gtcccacttg ctgcctgctt tgcttttgga gcagcgttag gtccgacaga
     1021 tgcggtagca gtcagttctc tttctggacg agtgaatatc cctaaaaagg cgatgcatat
     1081 tttggaagga gaaggtcttt tgaatgatgc atctggtgtt actgccttcc agtttgctct
     1141 tggtgcgctg atcaccggat ctttttcagc agtaaacgcg gggatgagtc tagtagtttc
     1201 tagtattggg ggagctttga tcggcttttt gcttgtctgg ttcaaacaaa aaataattca
     1261 tttgatcgaa aaagcatcag cacaagatgt cacagggtat ctgctgatcg aacttttgct
     1321 tccttttcta gcctatgtat tggcagaggc atttgatgta tctggaatca ttgctgcagt
     1381 ggcagcaggt attctccaag cttctggttt taggaaaatc tctgtgtttg atgcagaatt
     1441 gtcgagcttg tcccacagta catggacaac tattgctttt acactgaatg cattagtatt
     1501 tatattttta ggaatcgaat tgacgcaagt gttctcgcca gtatggggag acggtttata
     1561 tccaaacggt cttttattag cgattatcgt attaatctct gtcatgcttt ttgtgatcag
     1621 attcatttct atcagtcttt tttatgtatt caaagatggc agtaaaaagt tcaaaaaaca
     1681 gttgaacgag atcttgatct taacatttgg cggtgtaaag ggaacagtca gtttggctac
     1741 gatttttatt ttgccatttt caatcaataa tatgatgttt taccagcggt ctttgctttt
     1801 gtttctgacg gcgggtgtca tcttagtgac tttggtcata gggatcattg tcttgcctat
     1861 gctgacggaa acagaggaag cgaaaagcac ggatttgaat gctttgatga ttttagaaga
     1921 ggtcgttgaa gttttgcgaa aagaaataaa ggaaattgat cgtaatacaa aagaattttt
     1981 agccactgaa gcagtgatag aaaattatca agaacggata cgtgatctgt acttggaaga
     2041 tttaaccgat gatgaaaagc aagaagtaca agaaatacaa gcactgattt tgtcgatcga
     2101 acgggatgga ttggatgaaa gttatcgttc gggcaagctt tcttctaatg gttatcgttt
     2161 ttactcccgt tttctttctc gattcgaaca ttctatcaca agccagattt tgtcttttat
     2221 cggcttttgg ttcattgttg tccgtaggtt aatgcgaatc gttctccatc cgaaaatgtt
     2281 ttggcaaaga agatatgctg atcgtcagac atttattagt gatgaagata tccaagaaat
     2341 cagagagaca tatcaaaaaa atactcagtt gattattgaa agtttagata acttgactga
     2401 tatttatgat gaaacgatgg ttcaattttt tatccagcaa cgcaaacttg aaggaataaa
     2461 aatgaactcg gggaatttga tttcttcgtg gatgatccag caagatagtc tgtttacgaa
     2521 aaaaatgcta agaggttatt atcttgaaag aaaaatcatt gatgaatacg aagttgctga
     2581 aaaaatcacg acgttttctg cgaacagcta tcgcaggaat atcaatttgt tagaatctta
     2641 tacgatgaac aaaccatctg ataacttttc tttccgattt gcgtttcgag caaagtcaaa
     2701 aaaaacgagt ccaataaaat aaaaaggtaa aaattttcaa gttttataaa aaacaaaaca
     2761 tatcagaaca ggatcgaatc ggtcctgttc tttttttgag aacacaaaaa gaacaaatac
     2821 caaaaaaatt ttcctgaagt tcactatttt tatgatacaa tatccaagat aaggagggaa
     2881 catgttgaaa gaattaaaag taactgacct caaaaaaact tatggtgaaa aagatttgtt
     2941 tgaccagatt tcttttttgg tccacgaaaa agaccgtatc gggttgattg ggacgaacgg
     3001 gacgggtaaa acaagtctct taaatattat tgccggtatc gatagcagcg acggtgatcg
     3061 gcaaacagtg ttttatccaa ctgattaccg tattggttat ctttctcaag cagctgaatt
     3121 ttccgatgaa ttaactgttc tccaagcagt ctttcaagga aacagcccct tgatccaaac
     3181 tgttcgagca tatgaggaag cattgatcga actaggggaa aacggagaag accctgatgt
     3241 gcaaaaacgt tatgctaaag cagaagaaca aatgaataaa gaagatgctt ggactacaga
     3301 taccaacgca aagattattt tgcaaaaatt aggaatcagt gaattagata aaaaaatcag
     3361 tactttatca ggtggtcaga aaaaacgagt tagtctggcg caagtgttga ttgatgaacc
     3421 cgatttacta ttactagacg aaccaacaaa ccatttagat tatgaagcga tcgagtggtt
     3481 ggaaaactat ttgaaacaat accgtggcgc actattgatg gttacccacg atcgttattt
     3541 tctagataga gtagcaaacc gaatcttcga actttcattt ggaaaattat acgagtataa
     3601 aggaaattat gaagcttacg tcttagaaaa agcagaacgt gatcgtgtag aagtagaaca
     3661 agaagaaaag cgcaagcggc tttataaaca ggaattagct tggatgaggg ctggcgtaca
     3721 ggcacgtggc accaagcagc aagcacggat caaccgtttc gaagatctaa aggagaactt
     3781 gtaccaagta aaccaagaag atgatttaga gctgaaccta gcgacacaac gtttagggaa
     3841 aaaagtactg gaaatcaaag atggcagtta ccgtataaat gaccaaacac tgctagaaca
     3901 tttagattta ttgattcaat ctcgggaaag actaggtatt actggtaaaa atggtgcggg
     3961 aaaatcgaca ttgcttaata ttctagctgg cagaattccc ctggatagcg gtacaatgtc
     4021 tatcggtgaa acggttcatc ttgcgtatta tacacaagaa aatgaagaaa tggcaccaga
     4081 taaacggatg attgcttact tacaagaagc tgctgaagaa gcaaaaacag cagatggcag
     4141 tcaaatcgga gtagcagaat tacttgaacg atttttattt ccaagattta tgcatgggac
     4201 actgataggc aaactttcag gcggagaaaa acgccggtta tttttgttga aactgctgat
     4261 tcagcaacct aatgttttat tattggatga accgaccaat gatttggaca tagcaacatt
     4321 gacgattttg gaagattatt ttagaagttt tccaggtgct gtgatcactg tttcccatga
     4381 ccgttatttc ttggacaagg tagcagataa gttattagta ttccaaggaa acggaaaaca
     4441 agaactttat tacggaaaca tgtccagtta tttactgaag caaaaagaga cccagcagcc
     4501 tgctgaaaaa gcgaaaccaa aaatacagag caaggaacct gctgaaaaga agaaattatc
     4561 ttacatggaa caaaaagaat gggaaacgat tgaagatgag attgcagaac ttgaagaaaa
     4621 aatctcattg ctccaagaag agatgaatca tcaaggagat aatttcactc gattacaaga
     4681 attgcaaaac gacgtatctg aaacagaagc acaacttgaa gaaaaaatgg cacggtggga
     4741 atacttgagt gaatgggtag aagattagga ggaaaaagga tggaacaagc gtatcttgac
     4801 ttaggaagaa aattgttgga agaagggcat cgtaaagaag acagaacagg cacaggcaca
     4861 ttaagcttat tcggatacca aatgcggttt gatctgcaaa aagggtttcc tttgttaaca
     4921 acgaaacgag taccttttgg attgatcaaa agcgaattgc tttggttttt aaaaggagat
     4981 acgaatatcc gctatctatt acaaaataaa aaccatattt gggatgaatg ggcatttgaa
     5041 cgctacatca aaagtccaga ctataatgga ccagatatga ctgattttgg ccatagatgt
     5101 ttgacagatc ctgaattcaa tgaagaatat aaaaagcaga gcaagctttt ttgcgagaag
     5161 atcctaaccg atgattcctt tgctgaaaca tacggcgatc ttggacacat ctacggctat
     5221 caatggcgtc actgggaaac aaaagacggt ggctttatcg atcaaatcaa acaagtgatc
     5281 gaagcaatca aaaagactcc tgattctaga agattgatcg tttctgcatg gaatccggaa
     5341 gatgttccta gcatggcttt gcctccttgt catacgatgt ttcaatttta tgttcaagag
     5401 ggacggctaa gctgccaatt gtatcaacgc agtggggatg tgtttctagg cgttccgttc
     5461 aacattgcaa gttatgcgtt gctgactcac ctaattgcac atgaaacagg tttagcggtt
     5521 ggcgaatttg ttcacacatt aggagatgct catctttata tcaatcatat cgaacaaatg
     5581 aaagaacaat tgagccgaga aatacgcgca tttccgactt tagttttgaa tcaggaaaag
     5641 cagtcagtat ttgattttga gatggaagat atccaattag aaggatatga cccacatccg
     5701 gcgatcaaag ctcctatcgc tgtttagaat cagaaaggat gagaaaatgt tagctgcaat
     5761 ttgggcacag gatgaaaacg gattgattgg aaaagaagac cgtcttcctt ggcgtttgcc
     5821 aaatgatctg aaattcttca aacaaatgac agaagcaaat acactagtga tgggacgaaa
     5881 aacattcgaa gggatgggta gccgtccgtt gccgaatcgt cagacgattg ttttgacgag
     5941 agattcttcc tataaagcag aaggcgtgca tgtcatgcat agtgtggaag acgtgcttgc
     6001 tctggaaaaa gaaacagacg gtatcttttt catcgctgga gggtcagctg tttatcaaga
     6061 atttctgcca ttttgtacga tattataccg tacagtcatc catcatgtat tcgatggaga
     6121 tgcttatttt ccacctgttg actggtctga ttggtcactg atcaatacta gtcaaggaga
     6181 aatggatgaa aaaaatcgtt atccgcatca atttgaaacg tatcagcgta atgagaatcc
     6241 tgtaaaaagc gggacaggcg aattgacgat ataaaaaagc tatggataga ctagctgtta
     6301 tttagagctg tcccatccat agctttattt tttttattaa gtaaagaata aaacagagaa
     6361 gtacataaaa cctgcaccta acataacaaa caaatgccaa acaacatgca tatagcggat
     6421 agaattcaag ctataaaaga atgcgcctat cgtgtaagaa agcccgcctg ctaccatcaa
     6481 aataacacca gtcattccaa gagagtgata aagaggaaca gcagccacga cacagagcca
     6541 ccccataaaa atatagatga tcgtagatac ttttgatact ctctcccgtt tatgcaacgt
     6601 caacgcttta tatataatgc caagaatcgc aaacaaccag attaatacaa ataaagtcca
     6661 acctagccat cctcggatac tcagcaaaca aaaaggagta taacttcctg caatcaaaag
     6721 aaaaatcgaa ctatgatcga atacttggaa cacctttttt gcttttgtaa aaatcaatga
     6781 atgaaacaaa gtagacgtta aaaataaaag aatcatcatc gatccataga tagcataaga
     6841 gacaatatgg agggaggaat gcaagtgggc accttttact aaaagaacca ctagtcccgc
     6901 gatactcaat ccagtcccaa ttccatgggt gactgcatta aacacttcat ttaatatttc
     6961 atacgttcga ttaaatttcg ttttttccat gaaaacggct cctttactag gtgtaattaa
     7021 tgaaaaatca ttatttgtct gttatactaa aaatagaaat tgtcttacat gtagtttaac
     7081 atgaataagg catcttgtta aagaaagaga gagagaaaat aatgacaaat gtaaaaatcg
     7141 taacagattc ttcatgtacg atggaaaaaa atgtacgcga tgctttaggc attcgtgtca
     7201 tgccgctatc catcatgatc gacggaacag tatatcccga tgatgaccaa ctagacggcg
     7261 aaagatttat ggaaatgatg gcgaaagcaa aaaatctgcc caaaacaagc caaccaccaa
     7321 ttggagaatt catctcgatg tatgatgagc taggagaaga cggcagtgaa gtgatttcga
     7381 tccatatgac aaaaggcttg agtggaacag tagaagcagc aagacaagcc agtaatctaa
     7441 caaaaacgaa agtaacggtt atcgacagtg ataccactga ccaaggatta tcttttcagg
     7501 taattcgagc agctgaatta gcaaaagaag gtaaaacagt agagaaaatc cttgaagaag
     7561 tcaaaaaagt acaagacaat acaaaattat acatcggaat atccacacta gataacttgg
     7621 taaaaggtgg aagaatcagc cgaacaactg gtttgctgtc tagtgttttg aacatacgcg
     7681 tggtgatgaa tttcgcccat tctgaattaa tccctgtaac taaggggcga ggaaagaaaa
     7741 cgttcgataa atggtttaat aatttgaaaa atgaactgaa agaattgcca aatgtgcgac
     7801 aaatcggtat ttcacatgcg gatgcaaaag aattggcgca agaattcaaa gagggtttgc
     7861 aagaattatt cccggacatg catatccctg tactgcatac gaatccggtt atcgctacgc
     7921 atactggtaa agacgcgttt gcaatcatgt attatgtaga ttagtcgaaa agaggataag
     7981 ccctcagcct gaaaggcttg cagcttattc tctttttctt tataaaatta ggaaaataat
     8041 tgttttcttt tttcataaag actacctttt tgataaaatt tttgataaca ttagaaccaa
     8101 tcgaatacaa gaggtgaaaa aatgaaatta acgaaacgac atttgatcct cgttttgttc
     8161 ccattattaa ttgcgatcat catctatgca ggtttagctg cttctatacc gaaagctggc
     8221 ccactaatga aaccagtaac tactgtgaga gaagatccaa ataaaaaaga aaaacttcac
     8281 tacatagcta tcggagattc gctgactgaa ggagtaggag atcaaaccaa gcaaggagga
     8341 ttcgttcccc ttgtcgcaaa tgatctaaaa gaccgttata atttgaccgc ggttgaaaca
     8401 gaaaactatg gtgtaaatgg tgaacgaagt gaccagatct taaagcgagt aaaaacaaaa
     8461 gaagaaattc gaaacaatat caaaacagct gattttatca cactgactgt tggcggtaat
     8521 gatttgatga aagttatcca aaataattta ttcgggttaa cggtcaattc ctttaaaaag
     8581 ccactgaaaa actatcagga aaatgtcaca gaactattgg aagaatttcg atccctcaat
     8641 aaaaccgcac ctatttacat tttaggcatc tacaatccat tttatttgaa ctttcctgag
     8701 atcactgaca tgcagacaat tgttaacaat tggaacaaag gaacagaaac ggtgacaaaa
     8761 aaaatgccga actgctactt tatcccaatc aatgatttgc tttatcaagg attgaatcag
     8821 gaagtaggtg tcacagaaga aggaacagat caaacaacta gcacgacaga aaatctaaat
     8881 ggcatcaaaa ataatgtgct ctatgaagaa gatcatttcc atccaaataa tttaggttat
     8941 caattgatgg caaatgcagt gagagatcag ctgatcgaga cacaaaacca atggttagag
     9001 aaggaggatg atcaataaac atgaaaacaa gatctgacgt atcttcaaac aaaacaaaga
     9061 aaacaaatta ctggaaattc gccttcctta tccttcttgg ggtagtactt ggttcgaccg
     9121 tatttttagg cagccgtatt tttgctaatc gggaaccaga actcccagaa ataccagctc
     9181 ttacggaaag acaaggagat cctgtcttaa cgatcaattc aaataaagaa aaagtcaatc
     9241 aaatcatcag ctttttcctt agtgagtatc aaaaagatag tgatattgaa tataaatttt
     9301 atttggaaaa tgaagcacta ctcaatggta ctttcgaagt actaggtttt cctatcgatt
     9361 tctatttata ttttgatccg tatgtgatgg aaaacggaaa tgtccaacta aaagctagaa
     9421 gtctttctat cggaacattg agtttgccaa tcaaagatgt gatgaatatg atcaagagaa
     9481 actataaatt accagattgg atcgaagtca atacagatga tctgactatc atgcttaggt
     9541 tggatcaatt ccgaatgcaa aacgggatgt atattaaagc agataaaatc gacttagtaa
     9601 atgatgacat tcggtttagt ctatatttgc caaaagatac ctctgatgct tcaaacaact
     9661 caaataccaa caaaactagc accagcagta ctgaaagcaa gacaaaagaa aataaaaaat
     9721 catcggatga gtagtattag agggggaatc ataatggaac gagcaatatt tgcaggaggc
     9781 tgcttctggt gtatgatcca gcctttcgac acgcttcctg gtatccatac agtgatgtca
     9841 ggatatactg gagggcatgt accagatccg acttatgaac aagtaaaaag ccaaacgact
     9901 ggacatacag aagcagtgga gattatctat gatccaacat tgatttcata tgaacaatta
     9961 gtagaacttt actggcagca aactgatcca actgacgctt tcggacaatt cgaggatcgc
    10021 ggtgacaatt atcgcccggt gatcttttat ttgacagaag aacagcggat cattgctgaa
    10081 aaaagtaaga aacagttagc tgaaagtgga cgtttcgaac agccaatcgt tacaaaaatc
    10141 gaaccagccc aaccatttta tctagcagaa gaagaccatc aagactatta taaaaagcat
    10201 cctgaaaatt ttaaaagaaa ccatgcacgc cgggctgcct ttatcgaaga aaattggagg
    10261 aacgaagggt gaatcgaagc ttttttcatt atttaatgac actgcgaggc ggtggcacac
    10321 atgatgcttt atctgttttc gctacagaag ccggtaaaga catccaattt cctaagcaca
    10381 gctctagcta cgaagaaatt tctaattatc tggaactgaa tgtcgactat ttgccgtcca
    10441 tggatatctt tgacgaagca tgggaaaaat atttagaaaa taatcatgac atataagaga
    10501 tagttttgta ctttatgatc aaaaaagttt tttgtccaat agcctaattg aaagaattat
    10561 ttttatttca gctatctagt cgcatttttt tgatattttt cttataataa agaaagaatg
    10621 aaaaaaagag ggatggattg atagagattg acaaaaaaac aacgctatat taatcgcttc
    10681 tggcttgtat tcaaatactt acttgtttct gtattccttg cgttcatgtt gcggggattt
    10741 ttattcatcc cggtaccagt ggaaggcaat tcaatggaaa acgtgttgaa gcaaggtgat
    10801 atggttgtga tggaaaaatt ttcggagatc agacgatttg atattgttgt ttttcaatta
    10861 gcagatggca cgatttatat caaacgagta atcggtcttc caggagaaaa tgtcagctac
    10921 caaaatgatc aattgaagat caatgggaaa gtagttaagg aaccttatct aactaaaaac
    10981 ctgaaatcag atcatgcaaa tgcatcctat acgacggact ttacactaca agagctaacg
    11041 ggacaatcca agcttccgga agataattat tttgtacttg gagataatcg aagagtttca
    11101 aaagatagtc gttcgtttgg cacgatcaat aaaactgata ttctaggtaa agcaagattt
    11161 gtctattatc cacttgatga aataaaatgg atccagtgag aaacaataaa gcgtgcagtc
    11221 tgttgagact gcacgcttta ttgtttaatc caaaatcagt ttactacctt agtacgtaaa
    11281 atcaaacagc tcgtttaaac acttttctag cctctaaagt tttgataaat gaataaacaa
    11341 ccattatttg aatagggatt tggatcatat tcctgatgaa tctacctaga aaaattactt
    11401 tgaaatctgc accaaacaaa acagacatca gccaagaatt gataaaaagg ctgactggga
    11461 tcataatcaa taattgggca gcgatgacgg aaggcaggtt tatcttttta tgatataaaa
    11521 ataagccatt aataaatcca ccaaggaaag ccgtgataag aaaagcagct tgaaacgttc
    11581 ctgaaccaaa tatcgtgaaa tttaaagtgt tcgacaacat ttcaagcaaa ccagcccaga
    11641 tcggtccata tagataaccc ataagcgcca tacttaaaaa tccgaatgtg atttgaaaat
    11701 aagcaggacc aatcgctatc ttgtataaga tgacatcaag ggctaataaa agagcagagg
    11761 tcgacaacaa tcttgcattc atttcgggca atctaaaaac gtcttttgca atcaaagtgt
    11821 tcaaaatatt ctctcctttg tcagttaata ataaaaggat accataaaat caccaatgtg
    11881 tcatgacaca ttggtgattt acatacactg gcatctaaca attaaaaaaa gggtgagaag
    11941 actgctttta taaactgtaa aaaagagatt gtacgaataa aaaaaataga ggcggagaat
    12001 acttatcgta tcatccgcct ttgttttttt gttataaaaa acgtcagatt agttatctgc
    12061 ttacatcttc atccatacgg gttctaatgt atttccgtct agatcttgga cttctaatga
    12121 aaacatttct tcttcaggca ttcccatatc tacttcgtgg aaagtaccac cattttcttt
    12181 tgcaatttga gcaaattttt ttacttcatc cgcattttca acactaaacg aaatcaaagc
    12241 accacttgtt tttttattat cagctatttc tttatctttt ataaattttt tataaaaatc
    12301 atgagagaga agcattatcc aaaaagtgtc atcccatacc attgaaccag cttcttcagt
    12361 agaaaattct tcattttttt taaaacccag cttttcataa aaagcaacgg aacgattgac
    12421 atcagcaaca ggaaaattaa caaaaaccat actattcatt tgtttcgtct cctttctgtt
    12481 ttgttcgtct atcatctctt atttaggaaa cgctgtcaaa aatttagatt catatataga
    12541 aaccaaaata aaaatttgca tcatcaaaaa agtgaagatg tgatagaatg aggactggtt
    12601 cactttcact taactaatag atggaatttc tttataataa aaaagtgaat ttatattgaa
    12661 aaagaaaaaa acaagtaaag ccggggaata ttccggtatg aaaggaatgg agagaacaat
    12721 gaccatccaa tggtttccgg gacatatggc aaaagcccga cgagaagttt ctgaaaaaat
    12781 gaaatatgtg gacatcgtat ttgaattggt agacgcacga ttacctttat cttctcgtaa
    12841 tccaatgcta gatcagatca ttcagcaaaa acctcgcctt gttttattga ataaagcgga
    12901 tctggcagat ccacaacaga caaagctgtg gcaagaatat tttcgtaaac aaggccatct
    12961 tgcattagct atcacggcac aagaaagtaa aggaattaaa ggtctagtac ctgcggctaa
    13021 agaagcattg aaagaaaaac gggcaagaga tgctgctcgg gggatcaagc ctcgcgctat
    13081 tcgggcaatg gtcttaggta ttccaaatgt tgggaaatct acgttgatga atcgactagt
    13141 aggcaagaaa attgcacaga caggaaataa gcctggcgta acaaaaggtc agcagtggct
    13201 tcgtttaggt aatgagttgg aattgttaga cacaccaggg attttatggc ccaaatttga
    13261 agatgaagaa atcggcaaaa aactagcgct gacaggcgcg atcaaagacc agcttgtcca
    13321 tttagatgat ttagcgattt acggattaga cttttttgcc cgatattatt caggaagaat
    13381 cagcgatcgt tatggtttat caatcgagca agaagaaaaa ttggcaccgg aattactgat
    13441 ggatattaca gcaaaacgtg ggtataaaga agactatgat cgtggcagcg aattagtcgt
    13501 gcatgatatt cgtcaaggaa aacttggacg atacacactg gatcgttgcg aagaaatgga
    13561 gcaaaaaagt gatgactgaa tccatccaaa gtatcaaaga acgattgaaa agcatacaga
    13621 gaatagatga tcctacacta gaatcattaa gaaatgatcc acgcaaaggc gtgcagcaag
    13681 cactggcttc ttttgaacga cgattgaaaa aggaagcatt aattaaggaa aaatatgaag
    13741 aaatgttttg ctttgaaaag caagggtacg cacaaggtca ccaatggatt gccggcattg
    13801 atgaagtagg aagaggacca ttagctggac ctgtcgtggc agctgcagtt atcttgccga
    13861 aaaatgagat catattaggg ctgaatgact ccaaacaatt atcagagaaa aagcgagaac
    13921 ttctttatca acaagttcaa gaaaaagccc tggcaatcgg gatcgggatc gttgatcagc
    13981 agacaatcga tgatgtgaat atttatgaag caagtaaatt agcgatgatc caagcagtag
    14041 aaaaattgtc cattgtacca agctatttgt tgatcgatgc gatgaccttg gatttgccta
    14101 ttcagcagga aaaaataatc aaaggggatg cacgatctgt gtcgattgct gctgcaagta
    14161 tcattgctaa agtatatcga gaccaattga tgaaagaata tgacgtgctt tatcctggat
    14221 atggtttctc gcataatgca ggttatggaa caaaggaaca cttagaagga cttgaacgac
    14281 aaggaattac gccaatccat cgtaaaagtt ttgcacctgt aaaaaaatat ttacaataaa
    14341 aaacagccgc ttttacgtat cttattagaa agagagacga aaggcggttt tatgatgtat
    14401 caaatagaag aaaatttatt gaaacttgct tatacaaaag gaatcagtag tcaaggaaaa
    14461 tgggcacttg ctaactggat gatgcagtat ccttataaag aaatcggatt tgaagacatc
    14521 attaagatag gtaaaataac caaacaccgg gagctgtttc gcaaaagttg gcaggaaatt
    14581 cacgctaact ggggcaaaat ccaatcgaaa cagtcgttta tcacttgctt tgatcccaaa
    14641 tatccccctc agctgcttca cttgacctat ccgccaattg tactgtttta taatggtgat
    14701 ctatctttac tttcttgtaa catgttaagc gttgttggtg gaagacacac ttctgcttta
    14761 gccaaaaaaa cagtttatta tttactaaaa ccagtgatag aagctggata tgtgatcgtc
    14821 agtggctgcg cgaaagggat agatacatac ggtcaccaag cagcaatctc tcatggtgga
    14881 cggacaatcg cagtcattgg aacagggata gagcaagtct atcctgctga aaatcgtgac
    14941 ttgcaagagg tgattgcaaa tgatcatctt cttttgagtg aatatcctcc tcaatccggc
    15001 ccgaaaaagc attgttttcc aatgagaaat cggattatcg cagctttgtc taaaggaaca
    15061 tgtgtctttg aagccaagaa aaaaagcggc tctctgataa cagcacagca agcattggat
    15121 cttggaagaa cagttttttc tgttccagga aatattttaa cagaacattc taccggatgt
    15181 catcatttga tccaagacgg tgcagtttgc acggtttcgg gtgaagatat cttggcagaa
    15241 tttaaagaat aacaccatac ttttcatttg cttttcttga caaacgtttt ctgaatggat
    15301 atgattagct acgatttgta tgaatcctct tttaatagta ggaacacgac aatggaaagg
    15361 agtccattca gtaatggcgt ataaatattt agtgattgta gaatcacctg caaaagccaa
    15421 gacaattgag aaatatcttg gacgaaacta taaagtaatg gccagtgttg gccatataag
    15481 agatttaccg aaaagtaaaa tgggtatcga ttttgaaaac aattatgaac cccattatat
    15541 ttctatacgc ggaaaaggcg atgtcatcaa aagcctgaaa gccgcagcaa aaaaagctca
    15601 aaaagtttac ttggcaagtg acccggatag agaaggagaa gcgattgctt ggcatttagc
    15661 gtacctactt gggttggatc tgaaagaaaa aaatcgggtg gtcttcaatg aaatcacaaa
    15721 agacgcagtc aaagcagctt ttaaggaacc aagaacgatc gatgtagatt tagtagatgc
    15781 acagcaagct cgtcgtacct tagacagaat cgttggttat tcgatcagtc ctattctctg
    15841 gcgtaaggtc aagaaagggt taagtgcagg acgtgtccaa tctgtcgctt taaaaattat
    15901 tattgaccgt gaaaaagaga tccgagaatt tgttccagaa gaatattgga gcatcgacgg
    15961 taattttaaa aaagctcgca agaaattcaa agcaaatttc tggggaatcg acggtaagaa
    16021 aaagaaatta ccagatgcac aaagtgtaaa agaagtcact gctagaattg atggaacaga
    16081 cttcgaagtt aaaaaagtag aaaagaaaga gcgcaaaaga aaccctgcga atcctttcac
    16141 tacaagtagc ctgcaacaag aagctgcccg taagttaaac tttcgaacaa gaaaaaccat
    16201 gatggtagca cagcaattat atgaaggaat cactttgggt aaacaaggaa cagtcggctt
    16261 gattacatac atgcgtactg attctacccg gattgccgat acagcaaaag cagaggccgc
    16321 agcatttatt gaagatacgt atggaaaaga attttctaga catgatcacc gaaagatcaa
    16381 aaatgctcaa ggcgcacaag atgcccatga agctgtgcgt ccaacgagtg tgcttcgtac
    16441 accagatgaa ctcaaacaat atttagataa agatcaattg aaactttata cattgatctg
    16501 gtcacgtttt gtggctagtc agatgacacc agctatttta gatacaatga aagtgacatt
    16561 agaacaaaat ggtgtcatct tcattgcgaa cggttcaaaa atcaaattca aaggattcat
    16621 gcaagtgtat gtggaaggta gagatgacgg caaagaagag aaagaaaata ttcttccaga
    16681 acttgaagaa ggcgatgtag tccaatctgt tgatattgaa ccaaaacaac attttactca
    16741 accaccagca cgttttagtg aggctacgtt gattcgtgca ctagaagaaa acggggtagg
    16801 gcgaccttcc acttatgcac caacacttga tacgatccaa agacgctact atgtaaaatt
    16861 gacacaaaaa agatttgagc cgactgaact aggagaaatt gtcaattcat taatctgtga
    16921 attcttccct caaatcgtcg atatccattt cacagcagaa atggaaggag atttggataa
    16981 aattgaagaa ggcacggaag cgtgggtcaa agtggttgat cgtttctata aaccgtttga
    17041 aaaagaactg acgaatgcgg aagaaaaaat cgaaaaaatt caaatcaaag atgaaccagc
    17101 aggattcgat tgtgatgtct gtggacatcc gatggtgata aaattaggga aatatgggaa
    17161 gttttatgct tgcagtaatt ttcctgattg tcggaatacg aaaccaatcg taaaagaaat
    17221 cggtgtgact tgcccagtat gtcatgaagg acaagtcatt gaacgaaaat ctaagaaaaa
    17281 cagactattc tatggttgtt cccgtttccc agaatgtgaa ttcacttctt gggataaacc
    17341 agtgggcaga aattgtccaa aatgtgacgg atatctggtc gagaagaaag tcaaaggcgg
    17401 taaacaagta gtctgtgtaa atggagatta cgaagaagac atccaaaaat aatagaaaag
    17461 aggttctttc attgaaaatg gtaaatgttg ttggagcagg gcttgccgga agtgaagctg
    17521 cttggcagat cgcacaagca ggagtccctg tccgtttata tgaaatgcgt ccagtcaaat
    17581 cgactgaggc gcatcacaca agtaattttg ctgaactagt atgttccaat tcattaagag
    17641 gaaacagttt ggctaatgca gtagggttat tgaaagaaga aatgcgccga ttgaattcgg
    17701 tagtaatcac ttcggcagat gaaacagcag tgccagctgg tggtgcctta gctgttgacc
    17761 gcgattcttt ttctgaaaca attacccgaa aagtcaaaga gcatccatta gtaacagtga
    17821 taaacgaaga aattactgaa ataccggaag gaatcaccgt agtagcaaca ggacctttga
    17881 cttctcatcc attagcagaa agcatcaaag cattcaatgg ttcagatggt ttttatttct
    17941 atgatgcagc tgcaccaatc gtagacaaag ctacgatcga tatggataaa gtatatttga
    18001 agtcccgcta tgacaaagga gaagcagctt acttgaactg tccgatgaca gaagaggaat
    18061 tcaaagcttt ccatgaggca ctaatcaatg ccgaagtggc agaacttaaa tcttttgaga
    18121 aagaaaaata ttttgaagga tgtatgccaa ttgaagtaat ggcacagcga ggattcaaaa
    18181 cgatgctttt cggacctatg aaaccagttg gattggaaga tcctaaaact ggcaaacgtc
    18241 cttatgctgt tatccagctg cgtcaagaca atgctgcagc ttctttatac aacatcgtgg
    18301 gctttcagac tcacttgaaa tggggcgagc aaaaacgtgt tttccggatg attcctgggt
    18361 tagaaaatgc tgaattcgtt cgctatggtg tgatgcaccg aaacagcttt atgaattcgc
    18421 cagaattgct gaaaccgact taccaatcaa cgaaacgcga agatctgttc tttgcaggtc
    18481 agatgactgg tgtagaaggc tatgttgaaa gtgcagcgag cggactactt gctggaatca
    18541 acgccgcacg cttagcaaaa gaaatggaac cagtcgagtt tccacaagaa acagctattg
    18601 gaagtatggc ttactacatt actcatgcgg aaggaaaaca tttccaaccg atgaatgcca
    18661 actatggtct gtttcctgaa ctgccagagc gcattcgaga caaaaaagca agatatgaag
    18721 caattgcaca acgagcttta aaagctaatg aggcaataaa aacagaatta gaaaaaactg
    18781 caatcccaaa tgagtaagga aactccatga agagggtgag acggatagtc tcgctctttt
    18841 tcttgcgttt ttcttaagaa tttctcaaac aaaatgtgag gaattgcgcc aattgtgaac
    18901 aaacttagaa atattggtta aattctgaca atttatttgt ctttttataa acgctgtgct
    18961 aaattattct ctgggacaaa aggaaggtga gaaaatgaca gaagccacgt gggaacaaaa
    19021 atttttgcgt tatttgattg tcgaaagagg atattcagaa aaaacgaaag aagcatatga
    19081 agaagacatt ctccatttca aacatttttt ggaggaatct ggagacgctg atctgctgaa
    19141 ggcggaatat tttacggttc gtgtctattt gagtgcatta gctgatcatc gtcctagcta
    19201 tagcagaaat tctatcagtc gcaagatagc cagcctacgt tcgttttatc agtatcttct
    19261 caaagaagaa gtgataaaag aaaatccatt ttcttacgtt catctcaaaa agaaaaattt
    19321 gagactgcca cgtttttttt atgaaaatga aatgcaagca ctttttgaca gcgtagcagg
    19381 agatagtcct ttggatttga ggaaccgcgc tttactggaa gtcctttacg ggtcaggcat
    19441 ccgtttaagt gagtgtagca gtttgtcggt atcagacatt gattttgatt cagaggtcat
    19501 gttgatccat ggtaaaggga acaaagagag atatgctcct ctaggttctt ttgctcaaga
    19561 tgctctgcaa gaatatttca gggaaggccg ccaggtattg atgacaaaat atcacgaaga
    19621 gcatgatatc gtttttgtga atcatcatgg aaaaccaatc acgcctactg gaattgaata
    19681 tgtcttgaat caaatgatca aaaaaagcag tttggatagt gatatccatc ctcacatgct
    19741 gcgccataca tttgctactc atttattaaa taatggggca gatttgcgga cagtccaaga
    19801 attgctagga catgccaatt tatcgactac ccagatctat gcccatgtaa caaaagaaag
    19861 tttacaaaaa aactatcgtt ctttccatcc aagagcgtaa aaataaggaa aagaacaaat
    19921 ttaggaggaa acaaacatgg tcgaatcacc atttcattca accactatct gtgccgtcga
    19981 aaaagacggg aaatttgcta tggctggaga tggccaagta acaatgggtg aatctgttgt
    20041 catgaaaggc actgcaaaga aagtacgacg tatctacaat ggagaagttg ttgtaggttt
    20101 cgccggaagt gttgcagatg cgtttacttt agaagaaaaa tttgaaggca aactaaacga
    20161 atataacggg aatctacaac gcgcagctgt cgaattagct caagaatggc ggacacagca
    20221 atctatgcaa aaacttgaag cgatgctgat cgtaatgaat gataaagaaa tgcttcttgt
    20281 ttcaggtaca ggagaagtta tcacaccaga tgatggtatc ttagctatcg gatcaggagg
    20341 gaattttgcg ttggctgcag cccgagcaat gaaaaactat gctcaaaacg acatgagtgc
    20401 gaaagaaatt gcagaaaatg cgttaaatat tgccgcagat atctgtatct ttactaatca
    20461 taatattatc gtagaagaac tgtaagaggt gagtagatat gtatgaatta aaacaaacac
    20521 caagacaaat cgtaagtgaa ttagatgaat atatcgtagg acagcacgca gcaaaaaaat
    20581 ctgttgccgt tgctttgcgt aaccgctatc gccgtttgca gttagatgaa aaaatgcaac
    20641 aagatattac accaaaaaat atgctgatga tcggacctac aggggtcggt aaaacagaaa
    20701 tcgcacgacg tttggcaaaa atcgtcaatg caccatttgt caaagtcgaa gcaacaaaat
    20761 ttactgaagt cggttatgtc ggaagagatg tagaatcaat ggttcgggat ttagtagaaa
    20821 atgcgatcca aatcgtagaa aaagagcagt acagccgtgt atacacaagt gctaaaaaaa
    20881 atgcagaaaa acgtttggta aaaatcttgg tacctggtat caaaaaagaa cagaaacaat
    20941 ccactaatca atttgaacaa atgatgaatc tttttaatgc tagtcagcaa aataccgctg
    21001 aaccgcaaga agaagtgaca gaagatattc gaatcaatcg ccgtacagtc ttggaacaac
    21061 tacaaaaggg tttactggat agtcgtgaag taaccattga agttgaagac caaaagaaac
    21121 ctgcaccaat gatgaacaat ggattggagc aaatggggat cgacctaaat gaaactctag
    21181 gtgctttgac accgaagaaa aaagtggaac ggacggtaac agtcaaagaa gctttagagt
    21241 tattgatcaa ggaagaatca gcaaaattag tcaaggatgc tgatatccat agtgaggcaa
    21301 tcaaattagc ggaagaaagt ggaattattt ttatcgatga aatcgataag atcacttcta
    21361 aatcacagca atctggagaa gtatccagag aaggcgtcca aagagacata ttgccaattg
    21421 tcgaaggttc acaagtaaac acaaaatacg ggatcatcca aacagatcac gtcctattca
    21481 ttgcttcagg agctttccat ttgtctaaac caagtgatct aattccagaa cttcaaggac
    21541 gcttcccgat acgggtagaa ttagatgatt tgacagctga agattttgtg aagattctga
    21601 ccgaaccaaa taatgcttta gtaaaacaat acgtcgcgtt gatcggtaca gaaaatgtgt
    21661 cagttacgtt tacaaaagaa gcaattgaac ggatcgctca aatcgctttc gatgtcaacc
    21721 gtgacacaga taatatcggc gcacgcagac tgcataccat tttggaaaaa ctactagaag
    21781 atctattatt tgaagcacca gatatgcaaa tgggagaaat aacgatcaca gaagcatatg
    21841 tggatgacaa acttggctca attgcggcaa atgaagattt aagtcgctat attttataaa
    21901 aaatcggagg acgacaatga cagacttgct aacgaaaacc cgcaagataa acacactttt
    21961 gcagcaaaag aatacttttg acatgcaagc agatttgcct tacgataaaa tggctgtcac
    22021 actaggtggt attcttgaca gcaacaccta tatcatagga agtgacggag tattactagg
    22081 ttataatgaa cgtcacgatg tgaacaatga acgaatcaaa acaatgtttg ttgagaaaca
    22141 atttcctgaa tcttatactg atacagtggc acagttgatg aaaactgaag ctaatatccc
    22201 aattactagt gatattactg catttccggt agagtcaaga gagaaatatc catttggtct
    22261 gacgactgtc gttccgatct ttggagctgg cgaacgtttg ggtacgatta tcttatctcg
    22321 tatggaacaa tcgtttgcag atgacgatct agtcttagca gaatatggag ctacggttgt
    22381 cggtatgcag atcctttatc agaagtctcg aagcatcgaa gcagatgtac ggagtgcaac
    22441 ggctgtccaa atggcaatca acacattatc ttatagcgaa ttgaaggctg tacaagctat
    22501 ctttgaagca ttggatggag atgaaggtcg tctgactgct tctaatattg ccgatagtat
    22561 cggcatcact cgttctgtga tcgtcaatgc tttacgaaaa ttggaatcag ctggaatcat
    22621 tgaatctcgt tcgttaggca tgaagggaac ttatttaaaa gtattgaatt cccgttttaa
    22681 agacgagtta gcaaaacaca gttattagaa gtaaggggac tggggcagat agaaatgttt
    22741 gttccagcct tcccatttta aaaagtaagt cgaagttgga ggaatggcaa tgactgttat
    22801 tattcaaaat gaccagctga tcgccgaaat cgctgaacac ggtgcggaat tgatcagtct
    22861 gaaatcaaaa gaacatggtt tagagtatat ctggcaaggt gatccagcgt attggggcag
    22921 acatgcaccg gttttattcc cttttgttgg acgtttaaaa gatgatcagt atgtttatca
    22981 agggaaaaca tatcaaatgg ggcaacacgg atttgctcgg gatatggatt ttgaagtcat
    23041 tgaacatgga caagaaatgg ctagtttcat cttaaggagt acgccggaga cattagaaaa
    23101 gtatccattt gattttgaat tagtgatcag ctatgaatta ggaggagacg gcatcacggt
    23161 tcattatcaa gtggcaaata ccggtgaaaa agaaatgttc ttttctatcg gtggacatcc
    23221 tgctttcaat gtacctcttg aagaaggatt gtcatttgaa gatttctatc tggcattttc
    23281 tccattgaaa tcaagattac gcctaccatt gaaaggccca ttcatcgata tggatcaaaa
    23341 gacgttaggt cagacaaata cgaatctggc tttgaatcgt gaactttttg agcaagatgc
    23401 cttggttttt gaaacaaggg gtctaaatgc gtacgccatt cgaagtgaaa aatcttccca
    23461 tagcgtaact ttaagctata aagatatgcc ttatgtaggg atctggtctc cttatccaaa
    23521 agaagcgcct ttcgtttgta tcgaaccgtg gtgcggaatc gctgacacgg tgaacagtag
    23581 tggaaacttg atggaaaaag tagggatcaa tcaactaggt gcccatgaaa tcttcaagac
    23641 aaaatattcc atcacagtga agtaagaggt tgtgaccgag aagttagcag ggaacaccgt
    23701 ttaccaaggt gtgtaaaaaa gctatcctgt aagtaataag ggagtgtgac atcgatttgt
    23761 cacactcccg ttttatctct ttggaatgaa aaagtatcag gattttttct tgctgctatt
    23821 caaaccaaac gggattcgac tttcagtatg gtttctgata cgcttgatat tgtctttatg
    23881 ccgataaaag ataaaggaag aaactaaaaa ggcaatgatc gtcagtaacc aattgggctt
    23941 agccaaaatc acaggagcta cataaggaag gacgatcgta gataaagtga tcaatactgc
    24001 actgatcata ctgctcaaac tcaccatact tgtacaaaat aaactaacga taaagatgat
    24061 acaagaataa acaaaaaata caggattaaa tcctaaaagc attcctgcac ttgtcgcaac
    24121 agcttttcct cctttgaacc cagcaaaaat cggaaatgta tggccaagaa tggcgcatac
    24181 cccaaaccaa agagggttaa tcccagaaag gtggaagatc atcggcaaac aagtagccaa
    24241 tgtacctttt aaaatatcca taagcaggac aacaatcccg gctgttttgc ctaatacacg
    24301 aaatgtattg gtcgttccag tattaccgct cccgtgttgt cggatatctt ttttgaaaaa
    24361 caatttgcca atccagacac cggaaggaat agaacctaaa agatatgcta aaataagtaa
    24421 taagatcaat ttcatttttt accctctttc aagttgaaaa ctatccttta ttttagcatg
    24481 aataggtaac aggaacaatt gaaaaaatga aagatacaag tataatagat ggaataaaaa
    24541 gaaaagcaag gggtggcaga aatgtctgta aaaaatccaa cacaagaaac gattttcaac
    24601 tatttggaag aagcaaaaaa tattgccgtc gtaggcttga gcaataaacc agaacgaaca
    24661 agttacatgg ttgcacaggt attgcaagac aatggatata aaatcatacc agtaaatcca
    24721 ttattggctg gtgaagaaat tctaggcgag aaagtctatg cgaatttgac agaaatcgat
    24781 gatccaatcg atatcgtaga tgtatttaga cggagtgaat ttttacctga aatagcaggt
    24841 gattttttga aaactgatgc taaagtgttc tgggcacaat tagggattga aaacaaagaa
    24901 gcagctgaga tgctaagaaa agcaggacgt acagatatcg taatggatcg ttgtattaag
    24961 attgaacttg gtaagttgaa agaaaaccaa aaataaaaat gaccacaaag aagagatgat
    25021 cgatagaggg gaatgtcgaa aaagcggtta ggagataatt aaaattgatt tctcgtctac
    25081 cctctttttt ttcacgacct tttctagtat gataatcgtg gtgcggtttt gccgcaaagt
    25141 attattgaag gagcgtttgc cttggcaaaa aaagtaaaca acgaatacaa tgactcgtcc
    25201 atccaagtat tggaaggatt agaggcagtt agaaaaagac cagggatgta cattggatcc
    25261 actgacagcc gaggcttgca tcatttagtc tatgaaatcg tcgataatgc agttgacgaa
    25321 gcattatcag gttatggcag tgagattgat gtgaccatcc atgaagacaa tagtataact
    25381 gttgcagata gcggacgtgg gatgcctgta ggaatgcatg cttccggaat accaacagtt
    25441 gaagtgattt ttactgtgct gcatgctggc ggaaagtttg gtcaaggagg gtacaaaact
    25501 tctggtggac tgcacggtgt aggagccagt gtggtaaatg ccttatcgaa atggctaatt
    25561 gtaacgatcg ttagagatgg cgtagagtat cagcagaaat ttaaaaacgg cggtaaacct
    25621 gacggtactt tgaaaaagat aggaaaaaca aaaaaagcaa acgggacgac tgtccatttt
    25681 ttaccagatg atacaatatt cagtacaacg aaattttcat atgaaatttt agccgaacgg
    25741 ttaagagaat ctgcctttct tctaaaaggc gttaaaatct ctctttctga tcttcgtgga
    25801 gaagaaccag tcaaggaaat ttttcattac gaagaaggaa tcaaagaatt tgttgattat
    25861 ctgaatgaag aaaaagatac attgacacca gttgtttatt tttcaggaga aaaagaaggt
    25921 atcgaagtag aggtcgctta tcaatacaat gacggttatt cagaaaatgt tttatctttt
    25981 gtcaataacg ttcggacaaa agatggggga acccatgaag ctggtatgaa agcggcgatg
    26041 acgaaatctt ataatgaata tgcgagaaaa gttggtttgc tgaaagaacg ggacaagaat
    26101 ctggaaggta gtgatttcag agaagggtta gctgctgtac tttctatccg tgtacctgaa
    26161 aatcttctgc aatttgaagg gcaaacaaaa gaaaaacttg gtacgccagt tgctcgtacc
    26221 gtagtagata acgtcatcag tgaacaaatg ggcttttatt tgcaggaaaa tagcgaaatg
    26281 agccagatgt tggtccgtaa agcaatcaaa gccagagaag cgagagaagc agcacgtaaa
    26341 gccagagaag aaagccgaaa tgggaagaag cgtaaaaaag gcgaatctct tctttcagga
    26401 aaattgacac ccgcacaatc ccgaaaccct aagaaaaatg aattgtattt agtcgaaggg
    26461 gattctgctg gcggatctgc taaacaagga agagacagaa agttccaagc aattctacct
    26521 ttacgaggaa aagtcatcaa taccgaaaaa gctaaaatgc aagatatctt gaaaaatgaa
    26581 gaaatcaata cgatgatcta tacgattggt gcaggagtcg gtcctgagtt ttctatcgaa
    26641 gactgtaatt acgacaaagt aatcatcatg accgatgcgg atacagacgg cgcccatatt
    26701 caagtattac tgcttacttt cttttatcgt tatatgaaac cgctgattga agctgggaaa
    26761 gtatatatcg cgttaccgcc tttatataaa gtatccaaag gacaaggaaa gaagcaagtc
    26821 attgaatatg cgtggacaga tgacgaattg gcagcaatga ttaaaaaagt cggcaaagga
    26881 tatatgctgc agcgatataa aggacttgga gagatgaatg cggaacaatt gtgggaaaca
    26941 acgatggatc cgacttcccg tacgttgatc cgcgtccgta tcgatgatgc tgcgcaagct
    27001 gaacgtcgag taaccacact gatgggtgac aaagtagaac ctcgaagaaa atggatcgaa
    27061 aatcacgtgc aatttacgct cgaagaagat ggcagtatct tagataaaaa agaggatact
    27121 gaaatctctc cttcagtttc taatgattta ttagatgaag aacgagcaga taaaaatgaa
    27181 aacaatcagt tatttgaagt agaataggag gtcacctgat ggaaaatcga caagaagtac
    27241 aagaattaac tttagaagaa gtgatgggtg accgcttcgg acgttattcc aaatatatta
    27301 tccaagaacg tgcactgcct gatatccgtg atggattgaa gccagtgcaa cgccggatat
    27361 tatttgccat gaacaaagat ggcaatacgt atgacaaagg attccgtaaa tcagcaaaat
    27421 ctgtcggaaa tatcatgggg aattatcacc ctcatggtga ccgcagtatt tatgaagcga
    27481 tggtccgcat gagccaagac tggaagttgc gggaagtact gatcgaaatg cacgggaata
    27541 acgggagtat ggacggtgat ccacctgctg cgatgcgtta tactgaagcc agattatcca
    27601 agttaagtgg agagatgcta gcagatatcg agaaagaaac agtcgatctc gtgtggaatt
    27661 ttgatgatac agaaaaagaa ccaacagtcc ttccagctcg atacccaaac cttctcgtca
    27721 atggatcgac aggtatatct gctggatatg caacagaaat cccgactcat aacttagctg
    27781 aagtgatcga tggcacaatc tatatgatcg atcatccaca agcgagcctt gaaaaattga
    27841 tggaatatat tcctggacct gactttccaa caggagggat cttgcaagga aaagatgaaa
    27901 tcaaaaaagc ttatgaaaca ggacgaggaa aagttattct tcgttcgaaa acaaagatag
    27961 aggcaatgaa gggtaataaa cagcaaatcg tgatcacaga aatcccatat gaagtaaata
    28021 aagcgacctt ggtgaaaaaa atggatgaga tccgtttgaa taagaaaatc gatggaatag
    28081 ctgaagtacg agatgaaagt gatagaacag gtctgcaaat tgtcgtcgaa ttgaaaaaag
    28141 acgccaatgc tcaaggaatt ttgaattatt tgttcaaaaa cactgagcta cagatcaatt
    28201 acaatttcaa tatggtcgct atcgaccata tgactccgca tcaagttgga ctgaaagaca
    28261 ttttaagaag ttatatcgag catcgaaagc aagtcataac aaaacgtagt caattcgatt
    28321 tagccaaagc tcaaaaaaga cagcatatcg tagaaggatt gatgaaagct ttatcgattc
    28381 tggatgaagt aatcgcaacg attcgtgaaa gtaaagacaa aaaagacgcc aaaaagaatt
    28441 tagtcgacgt ctttcaattt acagaagaac aagcagaagc aattgtcaca ttgcaattat
    28501 accgtttgac gaacacggat atcacagagc ttcaaaagga atccgaaagc ttgattgcgc
    28561 aaatcacaga gttgaataaa atcctttcaa atgacaaaga gcttttctct gttatgaaaa
    28621 aagaattgcg agaagtgaaa aaaaattatt cttccgctcg tctaacgaca atcgaagacg
    28681 aaatcgaaga aatcaaaatt gatacgcaag tcttagtggc acaagaagac gtaattgtca
    28741 gtgtgacgcg agaaggctat gtgaagcgaa caagccttcg ttcttatagt gcttccaaac
    28801 cagaagaaat cgggatgcga gaaggcgact acttgctata ctcaggtgaa ttgagtacat
    28861 tggaccatgt tcttctgatc acaaataaag caaatgtgat ctaccgccct gtccatgaac
    28921 tacccgattt gaaatggaaa gatgctggtg agcacatttc tcaaacaatc tccaatctgt
    28981 ctgtagacga atcgatttta gctgttttcc catatcaaaa aattcaagca gagaaaacat
    29041 ttgtattcat cagcaagaat ggattgatca agcaaacacg aatgacagag ttcgaaccat
    29101 ggcgcaccta taagagccgt ccgctcagct gtatgaagct aaaggcggaa gaagatgaac
    29161 tgattgctgt gtatctgaaa gaagatcagc caggcttaga tgttttctta gtaacgcatc
    29221 aaggaatggg tttgcgctat cctttaacag atgttcccgt tgttggagca aaggcagctg
    29281 gagtgaagtc tatgaatttg aaagatggag ataggattat caacgggctg cttgtgttag
    29341 cagaaggtga tacgccgatc gtaatcgtta ctcagcgagg agctgttaaa cgcatgcttg
    29401 ctcaagaaat cagccaaact tcacgtgcga agcggggagt gacggtcatg cgtgagctga
    29461 aaaaaaatcc gcaccgtatt atctatatgt cggaaggaag atcaaaagaa ataaccttga
    29521 tgaatcaaaa agggcagcag ttaacgattg acccaactga tttccctata ggtgatcgaa
    29581 catcgaatgg atcatttgcg atggatgaga aaaaaggtgg agaagtcatc gaagttatcg
    29641 atgctcctga gattttcatc gagtcgtaac tgttgtagta ttttaatgaa gagacgccat
    29701 ctgtattaat acaggtggcg tctctttttc attcattttt aataaatcct tatacgtttg
    29761 caaaaaaaga tgaaagtgtt cacaatgctt aaatgttggt atgtcaacgt tgatttaaat
    29821 atatgtaaat aaaacataaa aattgttaaa taaaaaacaa gaaaaccctt gcaaagatag
    29881 ggtgctgtgt tatactaatc acgtaaaaac tgttacataa aacagaaatt taggaggtcc
    29941 atacaatgga gcaatggaat ggatttaaag gtaccaaatg gaaagaaggc gtagacacta
    30001 gagactttat tcaaaataac tacactgaat atcgcggtga tgacagcttt ttggaaggcc
    30061 caacagaagc aacagaaaaa ttatgggatg aattgcaaaa gttaaatgat atccaattcg
    30121 agcgtaacgg tgtctatgat atggacacag aaatcgtatc aactattact tcacacgatg
    30181 caggctattt ggataaagat ttggaaaaaa tcgtcggtct acagacagac aaacctttga
    30241 aacaagcatt catgccgttc ggcggaatca atatggctaa caatgcgcta aaaagcaatg
    30301 gctatacacc aaacgatgaa ttgacacata ttttcactga ctggcgcaaa acacataatc
    30361 aaggtgtatt tgacgcttat actgctgaaa tgcgtgctgc tcgtaaaaac aaaatcatta
    30421 cagggcttcc agacgcttac ggtcgcggac gtatcatcgg tgactatcgt cgtgtggcat
    30481 tgtatggaat cgactatttg atggaacaaa agaaaaaaga tcatgatatg actggttatc
    30541 atacaatgag tgaagaagtg attcgtttac gtgaagaaat cactgaacaa tatcgtgcat
    30601 tagcgcaaat gaaagaaatg gctgcgaaat atggctatga tatttctaaa ccagctgcaa
    30661 atgcaaaaga agctgtccaa tggctatact tcggatattt agcagcaatc aaatcacaaa
    30721 atggtgctgc gatgtcaatc ggacgtatct cagcattcct tgatatctat atccaacgtg
    30781 atttggataa tggagtaatc acagaaaaag aagcacaaga attgatcgac cacttagtaa
    30841 tgaaattacg tatggtcaaa ttcgcgcgta cacctgaata taaccaatta ttctcaggat
    30901 tccctatctg ggctacttta tccattgcag gtatgggatt agatggtcgt tcattagtta
    30961 caaagaatga tttccgtctg cttcatacat taacaaatat gggaccttca ccagaaccaa
    31021 acttgactgt tttgtattct gaacaccttc cagaaggatt cagaacatat gctagcagaa
    31081 tcgctatcga aagttcttcg attcaattcg aaaatgataa tcagcttcgt gaacaatggg
    31141 gttctgatga ttgtgcgatt gcttgctgtg tatcaggaac agttgtcgga aaagacatgc
    31201 aattctttgg cgctcgtgct aacttggcta aaacactatt atatgccatc aatggcggag
    31261 tcgatgaagt gactggcgcc caagttggac cagaatatcg tccaatcaca tcagaaatac
    31321 tagactttga agaagtgaaa cataacttca tgaatatgat ggattggtta gcagaactat
    31381 atgtcaatac gttgaacatc attcattata tgcatgataa atattcttat gaagcagctc
    31441 agctggcttt gatggattct gacttgcaac gcacatttgc tacaggtatt gctgggatct
    31501 ctcatgcggc agacagcttg tcagcgatca aatatgcaaa agtaaaagca atccgtaatg
    31561 aaaaaggaat agcagttgat tttgaagtgg atggcgactt ccctaaatac ggaaatgatg
    31621 atgaccgtgc agatgaatta gtttcatgga tcttggaata cttcatggga caaatcaaac
    31681 gtcataaaac ttaccgcaac tcaaaaccaa caacttcatt attgacgatt acttcaaatg
    31741 ttgtttacgg taaaaataca ggaaatactc ctgatggccg tgaagctgga aaaccacttg
    31801 cgccaggagc aaatccaagt tacaatgcgg aacaaagtgg gttattagct agcctgaact
    31861 caacagctaa aatgaattac ggctgtgcat tggatggaat ttcaaatacc caaacaatca
    31921 atccttcagg tcttggaaaa gaacaagata caagaatcga aaacttgcgt aatgtcttag
    31981 atggatactt ctcaaaagga gcttatcatt tgaatgtcaa tgtattcagt aatgatttat
    32041 tgcgtgatgc aatggaaaat ccaatgaaat atccgaactt gacgatccgg gtatctggtt
    32101 atgcagttaa attccgtgac ttgacaagag aacaacaatt agatgtcttg atgagaactt
    32161 cacacgacag aatgtaaaaa gttcgtgaaa aaagtttttc gacttagcgt cgaaaggacg
    32221 ttgaatagcc atcgcatcaa aatcgaataa tcagtatgct tatttgaaag aaacgattta
    32281 caggggcagg acttgtatgt tccgtccctg taaattttac acataacgaa gcggcaattg
    32341 cgaaaaattt tgttcagaaa gcttcgcttc gacagctaac ttgtacttaa aatgaaatca
    32401 aagaaaagat gtgaacttaa atggaagaaa aaacaatcgg ctatgttcat tctatcgaaa
    32461 ctttcggttc agtagacgga cctggactgc gctttgtcgt gtttatgcaa ggatgccgca
    32521 tgcgttgcca attttgtcac aatccagata cttggaatat tggcggaggg aaagaataca
    32581 cagcagatga acttttagat aaagcagaac gcttcagacc ttactgggga gataaaggtg
    32641 gtattactgt tagtggtgga gaacctctat tgcagatcga ttttttgatc gaactattta
    32701 aaaaagcaaa agagcggaag atgcatacta cattggatac atgcggaaaa ccttttacgt
    32761 atgaagaacc tttcttttca cgttttcaag aattgatgaa gtatacagat ttactgttat
    32821 ttgacatcaa acacatcgat aatgaagagc ataaaaaact aacgcattgg gacaatgaaa
    32881 atattttaga aatggctcaa tatttgtcaa aaatcaacaa accggtttgg attcgccatg
    32941 tactagttcc tgagcgaagt gattatgatg aatatttgat ccgactagac aactttattc
    33001 aaaccttgtc aaatgtagac agagtagaga ttcttcctta tcatacgatg ggaaaataca
    33061 agtgggaaac acttggtttg aaatatccat tagaagggat cgaaccacct acaaaagaac
    33121 gtgtcgaaaa tgctaaacga ttgcttcata cagaagagta tacaaaatat ttaacgcgat
    33181 aggtcgcatc ggaaagaaga taagtattgc ttctttccgt ttttttgcta ttattaaata
    33241 ggagaaatca caaaggagcg aatgacatgt caaaaatttt agtttttggt caccaaaatc
    33301 cagatacaga tgcaatcggg gcagccattg cgttttcaca cttgcaaaat gaattaggta
    33361 aagaaacaga agcagttgct ctaggtacac ctaatgaaga aacacaatac gcattagacc
    33421 actttcagct atctgcacca cgagtagtag agtcagctaa agcagaaaat gcagatgtca
    33481 tgcttgtgga tcataatgaa ttccaacaaa gtatttcaga tatcagcgaa gtaaatattt
    33541 tagctgtagt agaccaccat cgtattgcaa actttgaaac agctaatcca ttgtactatc
    33601 gtgcagaacc agttggatgt acaagtacga ttattttaaa actatataaa gaaaaccaag
    33661 taacaattcc taaagaaatc gcaggaatca tgctctcagc tatcatttca gatacattgt
    33721 tgttcaaatc accaacatgt acgcctgaag atgaagcagc tgcaaaagaa cttgcagcaa
    33781 tcgctgaagt cgatctaaac acttatggac ttgaaatgtt gaaagcagga acaaatctaa
    33841 gtgataaatc agctgcggtt cttcttgatt tggatgcaaa aagtttccca atgggcagtt
    33901 ccaacgttcg tatcgctcaa gtcaatacag tggaccttaa tgaagtgatg gatcgacaag
    33961 cagagctaga agcagcaatg gaagatgaaa tgaaagcaaa tggctacgat ctgttcgtat
    34021 tagttgtcac aaacatctta gacagtgatt cagaattact tgttgctggt aaaggaaaag
    34081 aaaaagtaga agaagctttc caaacaactt tgaccaacaa ccgtgctttg cttaaaggtg
    34141 tcgtttcacg taaaaaacaa gtcgtacctc aattgacggc tgtatttagc taatcaaatg
    34201 gtagtatgaa gcattcaaaa tgaatggata agcgattgat ataaaaagaa acatagaaca
    34261 tactaaaggc cgaagaaatg gaggagaata acagtcaatt atgactcttg ttcttcctcc
    34321 tttttacgta aaagcctttt aagtaaaata aaacggatag aaaagagaac atatatgaac
    34381 tattattcaa aaaataagga aaacttttat caaaaactga ccgatgatcc gcttttttct
    34441 tcattgaccg attatctata tgaacatcga gaacaagaaa cgattctaag agaattgaaa
    34501 aaagaatttt cgcaaaataa attttcccat tttcttgatt tattgatcga tgcaggatta
    34561 atcaagaggg aagaacgaag atatcatttg aactttccaa tatttgatcc taaagattac
    34621 ttgcaacaag ccacttcggc tgctgaaacc atagctgaac aattgaagag actctctgtt
    34681 gatgaacaaa aattagcgat gggagaagtt atttgggcgt attgtttcga agatgaacgg
    34741 aaagaagcgt atttttatgg agtaagaaac agccgagaga cagaattgct gaggacaact
    34801 gcaggaaatg agaagtaccg ttttatcaca ctttcctcta tagaacactt cccgcttact
    34861 ttggctaatt acttttttgt acagaagaat cagcttcctg taacgaaggc attcaaagaa
    34921 ttagccgaac ttattggaga cgtaaatgaa tcgtactttt ttgatcaaat agaagttatc
    34981 gttgacagga tacgtaagaa taaatataaa aatcgccggc ctagtatttt ccatcagtct
    35041 ctacttgtga cgaatactat aaaggaagaa gaatcattca cattagaatt acctattgtc
    35101 gaaaaaaaca attttgagat tgaattacca acattggatc cttcattgac aatggaggaa
    35161 atggcctttc ttaaacgaca aatcttttct gagctgagca agaaatttat accgcatgca
    35221 ttttcttata tcaaagaata tggaacagtt ttagtgagta aaacctaaga gagcagtaag
    35281 caaaaattat actgactctt gcaggaggag cctttctctt cctttatggt taaaagaatt
    35341 atttgagtat tttgcctatc taccacctat tagattcact acatggtctg gaaacaaagg
    35401 aatgcaaaaa aatggtgaga aatgtggaat gacaaaggaa gtagtgataa gaaaagttag
    35461 atttttaaat aatcaatatt atgattcagt caagtatggc attttatggg aagaactagc
    35521 tgattagatt aggaagtcca tttgataatc cagaatcact aagaaacgaa tggcgagtca
    35581 ttaataattt atccaatctt gggttattct tttagattga agtgtttaaa actaaaaaag
    35641 catttatgaa gtcgtaatag tcaggcttca aaatgctt
//
LOCUS       DACDIE010000026        33448 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.026, whole genome shotgun sequence.
ACCESSION   DACDIE010000026 DACDIE010000000
VERSION     DACDIE010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33448
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.026"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..1236)
                     /locus_tag="IV250_001359"
     CDS             complement(<1..1236)
                     /locus_tag="IV250_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8768653.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISI"
     gene            complement(1229..2128)
                     /locus_tag="IV250_001360"
     CDS             complement(1229..2128)
                     /locus_tag="IV250_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8768654.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            complement(2273..4051)
                     /gene="uvrC"
                     /locus_tag="IV250_001361"
     CDS             complement(2273..4051)
                     /gene="uvrC"
                     /locus_tag="IV250_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAP8768655.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            complement(4233..4547)
                     /gene="trxA"
                     /locus_tag="IV250_001362"
     CDS             complement(4233..4547)
                     /gene="trxA"
                     /locus_tag="IV250_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAP8768656.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            complement(4634..6994)
                     /locus_tag="IV250_001363"
     CDS             complement(4634..6994)
                     /locus_tag="IV250_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAP8768657.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            complement(7109..7657)
                     /locus_tag="IV250_001364"
     CDS             complement(7109..7657)
                     /locus_tag="IV250_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HAP8768658.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            complement(7715..8152)
                     /gene="zapA"
                     /locus_tag="IV250_001365"
     CDS             complement(7715..8152)
                     /gene="zapA"
                     /locus_tag="IV250_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAP8768659.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            8354..9271
                     /locus_tag="IV250_001366"
     CDS             8354..9271
                     /locus_tag="IV250_001366"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HAP8768660.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            complement(9649..10560)
                     /locus_tag="IV250_001367"
     CDS             complement(9649..10560)
                     /locus_tag="IV250_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP8768661.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(10961..11539)
                     /locus_tag="IV250_001368"
     CDS             complement(10961..11539)
                     /locus_tag="IV250_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8768662.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            complement(11559..12227)
                     /locus_tag="IV250_001369"
     CDS             complement(11559..12227)
                     /locus_tag="IV250_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8768663.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            complement(12242..13312)
                     /locus_tag="IV250_001370"
     CDS             complement(12242..13312)
                     /locus_tag="IV250_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8768664.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            complement(13483..14847)
                     /locus_tag="IV250_001371"
     CDS             complement(13483..14847)
                     /locus_tag="IV250_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP8768665.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            complement(14844..16403)
                     /locus_tag="IV250_001372"
     CDS             complement(14844..16403)
                     /locus_tag="IV250_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8768666.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            complement(16459..18939)
                     /locus_tag="IV250_001373"
     CDS             complement(16459..18939)
                     /locus_tag="IV250_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8768667.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            complement(18923..19258)
                     /locus_tag="IV250_001374"
     CDS             complement(18923..19258)
                     /locus_tag="IV250_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8768668.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(19272..20306)
                     /locus_tag="IV250_001375"
     CDS             complement(19272..20306)
                     /locus_tag="IV250_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP8768669.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(20396..21076)
                     /locus_tag="IV250_001376"
     CDS             complement(20396..21076)
                     /locus_tag="IV250_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP8768670.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            complement(21246..22205)
                     /locus_tag="IV250_001377"
     CDS             complement(21246..22205)
                     /locus_tag="IV250_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8768671.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLSYVTKDDLSNLI"
     gene            complement(22426..23529)
                     /locus_tag="IV250_001378"
     CDS             complement(22426..23529)
                     /locus_tag="IV250_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP8768672.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLRDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            complement(23751..24221)
                     /locus_tag="IV250_001379"
     CDS             complement(23751..24221)
                     /locus_tag="IV250_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAP8768673.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            24478..25797
                     /locus_tag="IV250_001380"
     CDS             24478..25797
                     /locus_tag="IV250_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HAP8768674.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            26087..26431
                     /locus_tag="IV250_001381"
     CDS             26087..26431
                     /locus_tag="IV250_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HAP8768675.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(26621..27121)
                     /locus_tag="IV250_001382"
     CDS             complement(26621..27121)
                     /locus_tag="IV250_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8768676.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            27649..27732
                     /locus_tag="IV250_001383"
     tRNA            27649..27732
                     /locus_tag="IV250_001383"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27683..27685,aa:Leu,seq:cag)
     gene            complement(28056..28694)
                     /locus_tag="IV250_001384"
     CDS             complement(28056..28694)
                     /locus_tag="IV250_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8768677.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPV
                     QAEQPVVQETVQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTAT
                     NGRYIGRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            29029..29463
                     /locus_tag="IV250_001385"
     CDS             29029..29463
                     /locus_tag="IV250_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP8768678.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            29486..29806
                     /locus_tag="IV250_001386"
     CDS             29486..29806
                     /locus_tag="IV250_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8768679.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            29947..30138
                     /locus_tag="IV250_001387"
     CDS             29947..30138
                     /locus_tag="IV250_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8768680.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(30209..30466)
                     /locus_tag="IV250_001388"
     CDS             complement(30209..30466)
                     /locus_tag="IV250_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAP8768681.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            30624..30887
                     /locus_tag="IV250_001389"
     CDS             30624..30887
                     /locus_tag="IV250_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8768682.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            30911..31099
                     /locus_tag="IV250_001390"
     CDS             30911..31099
                     /locus_tag="IV250_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8768683.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(31182..31367)
                     /locus_tag="IV250_001391"
     CDS             complement(31182..31367)
                     /locus_tag="IV250_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8768684.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            31582..31953
                     /locus_tag="IV250_001392"
     CDS             31582..31953
                     /locus_tag="IV250_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP8768685.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(32103..32175)
                     /locus_tag="IV250_001393"
     tRNA            complement(32103..32175)
                     /locus_tag="IV250_001393"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32140..32142),aa:Gln,seq:ctg)
     gene            complement(32221..32649)
                     /locus_tag="IV250_001394"
     CDS             complement(32221..32649)
                     /locus_tag="IV250_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8768686.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(32709..33440)
                     /locus_tag="IV250_001395"
     CDS             complement(32709..33440)
                     /locus_tag="IV250_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HAP8768687.1"
                     /translation="MFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSSTQKTKKTKEK
                     AEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDYVPVFNADGT
                     HGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLDDIKKALSKG
                     NPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIYVNDPYGVKN
                     RAVPINDFSAIFTAMGGQMLTLEKS"
BASE COUNT         9995 a         6619 c         5912 g        10922 t
ORIGIN      
        1 aattgaaatc gattgtttca atgaactcca taatccgcct tcgctataaa cgagtacatt
       61 cgatttgtac atgtttgcag acaagatcat caataagaat gttacggcta caagtaccaa
      121 caaggaaatc aatgctccac ccataccgac tgtctcattt gctaagcgaa tcggcataat
      181 atatgaagat aagaatggaa tatacgaagt gatccgaata acgatattgt ttggatctga
      241 agctcctagt atcaatccaa gcatgtatcc gcctagagat agataagtca ctggcaaaat
      301 tgcttttgct gtatcttctg ctttgttgac caaagagcca cataaagctg ctaaaacaga
      361 aaaaatcaaa atgccaaaga tcatgaatag aagagaatac cacaagaatg gtccaatcaa
      421 gttatccaat gagataccgt ctaatatatt tttgaccatt tccatatctt tgaattgact
      481 gtatcctata ccaaaaataa taccgtataa tgccatttgc gttaatgcaa caagtaaaac
      541 acctgttaat tttccgtaaa aatgcttttg tgcagtcgta ctggacagga tcacttccat
      601 gatccgcgtg cctttttcag aagcgatctc ttgtgcaatg atctgcgcat acgtcaaaat
      661 aataatgaac aagacaatcg ttgctacata acttacgacg tattggactg tcgagttatc
      721 ttctccgact gtcatttttc cttttgaatc aaaattgact ttttgtcgag tcaaacttgc
      781 ttgttggctc aagctagcta cttcttctgg tgaaatgcct aattgactcg ctcgaagcat
      841 cgattgcaag ccagtcagtt gttgttgtat cagtagttgt gtactttgtc ctaatgaatt
      901 ttcgctataa agcatcccat ttatcttatc attctcagtt gagactacta aatacgcatc
      961 gattttttct tccgaaagct ctttttgtgc tttttcttca gaatcaactg ctttgaactc
     1021 aaaatcatct gttttgactt ggctcatttg ctgactgatt tgctcattat cagccaatac
     1081 gccgatttta ttgacatcac tattttgttg agcaaagttt cctgctacat aaatgatacc
     1141 cagcatgata aaagggacta atatcatgat caagaaagaa atcgacttaa cattttttaa
     1201 atagacatct ttggtaataa tccaaaactt acgcatcgat ttcacctgct ttcaatttga
     1261 aaatctcttc caaagttggc ggttgctggt taaacatagg aatgtagccg aactgagtcg
     1321 cacgagtaaa gatttctttc ccagcttcag gatcttctaa ggtaatctct aacgtattgt
     1381 cttcatgaag gatcactttt ttcacgcctt cgatttcttc tacttcctgt tggcttaaac
     1441 cagattccaa gaataacttc gtacggccaa atgattcacg aatctcatgt acttttccat
     1501 tcaaaaccat ttttccgttt cgcaacatga tcaaatgatc gcagattttt tctacattat
     1561 ccatattgtg gctagaaaaa atgacacatg aaccatgttt tttcaattcc aatattccat
     1621 cttttagcaa ttcagcattt accggatcta acccgctgaa tggctcatcc aaaatgatca
     1681 atttaggttc atggattaac gtggcgatca gctggacttt ttgctgattt cctttagaca
     1741 aagattttac tttatccgtc tttttccctt ttacctgaaa tttttccatc caaaagtcga
     1801 tttttggttc aatctctttt cttgatttcc ctctaagttc tgcaaaataa agcagttggt
     1861 cttgaatcgt tactttcgga taaagtcccc gttcctccgg aagataaccg atgacatcat
     1921 aatctttctc cccaagatga tggccattcc ataacacttc tccttggtct tgtgtcaaaa
     1981 aatccaagat caaacgaaaa gtcgttgtct tcccagcccc gttttggcca attagcccca
     2041 tgatttttcc atcggggatt tggaaagaga catgatcgac agctgtgtag ttcccaaaag
     2101 ttttaacaag atccttgact tctaacattt cttcactcct tcttcaagaa atcacatata
     2161 tccttagaca ccagacgtaa gcgatttatt atttaccaac aaaacgtaaa cgctcttttt
     2221 gttgtacata aaatgatagg acactttact ttctttgtaa agtgcccttt cctcattcct
     2281 gatgtaattt atcaaatacg ttttgtgcga catttttcgg cagtccgatt tcttgtaatt
     2341 cttctgtcgt ggctgctgtg atatttttca acgatttgaa ctctttaagc agcatttttt
     2401 ttcgttttgg tcctaaccct tcaatttcat ctaatcggga agcaaaactg tttttactgc
     2461 gtagctgccg atggaaagtg attgcgaatc gatggacttc atcttggatt cgctggagta
     2521 agaaaaactc tggtgaattt cgctcaagcg gtactacagc caaatctgta ccgaatagca
     2581 actcatttgt tttatgttta tcgtttttta ccatgccagc aataggaata tcgattccta
     2641 attgattttc taagacatct tttgccacat ctacttgtcc ttttcctcca tcgatcaaga
     2701 tcaaatcagg taaaggcaac ccttcgcgaa tcactcgcga atatctgcga tagatgactt
     2761 ctctcattga tgcataatca tccggtccgt tgactgtttt tattttatat ttccgatatt
     2821 cttttcttgc cggttttcca tcaatgaata cgaccatcgc agccacagga ttcgtcccca
     2881 tgatattaga gttatcaaaa gactcgatac gtattggggt cgggatattc atcgcgtttc
     2941 ctaaacgttc aactgctccg atcgtccgtt cctgttttct ggcaatcaga tcaaaccgtt
     3001 cattcaaagc aactgaggca ttcttactcg ccaattcaac taattttttc ttttctcctc
     3061 gttttggttg cagcactttc gtagctaaca aagcttctac actgggcttg tcgatcgtat
     3121 caggaatcaa gacttcttta ggaatgaaat gctcattttc ctgataaaac tgaccgaggt
     3181 aagtcaagaa atcttcttct gcttcgttat agaaaggaaa cattgaaaca tctcgttcaa
     3241 ttagctttcc ttgtcttacg aaaaacactt ggacacacat ccatccttta tcaatcgcat
     3301 aaccaaatac atctcgatcc agtaaatcag tatttgtcat tttttggcga gtcatgattg
     3361 tctcaatggc acggatctgg tctcggtatt ctgcagcttt ttcaaactcc atgttttcag
     3421 cagctttttc cattttctga tggatctctg actcgatttt ttcgtgtcct ccgtttaaaa
     3481 agtttttcac ttcatcaacg attttcgtat agactttcgg atctacgtcg aaataataag
     3541 gacacaggca ttgtcccaaa tgatagtaaa gacaaggctc cttcgtctga ctaggtttgc
     3601 attttctcag aggaaataat cgatcgagga tcttctttgt ctcattcgcc gcaccgacat
     3661 cgggatatgg tccaaaatac agtgctttgt cttttagtac cttacgagta atcaccaaac
     3721 ggggatattt ttcattcgtt atcttaagga aaggataggt tttatcatcc tttaacataa
     3781 tgttgtattt aggatcattt ttctttatca gattgatctc taagagcaat gcttcgatat
     3841 tcgattctgt aacgatgtat tcaaagtctt cgatttcact aacaagccgt tccgttttcg
     3901 tattatggct accagtaaaa tatgagcgga cacgattttt taatattttg gctttgccga
     3961 cataaatgat cgttccattt ttatctttca tcaagtaaca gccgggctga tcaggaagta
     4021 aagctaattt gtttttgatt cgttcgttca tctggtactc cccttcatat tcaattaaag
     4081 ttattatagc atgtctgtca aaaaaacaat ccagcctgac aacgtaatta tactcaaaaa
     4141 atattaattt gaataacctt aactcaaccg caaaaaaacc gaggacggaa ctggattcag
     4201 tcccaccctc agcttatttt tgctgtaagt ttttataaat attgatcgat catttgacgt
     4261 agttgttctt ttgaatgaac accaacagct ttttctacta cttgaccatc ttttttcaac
     4321 attaatgttg gaatgctcat gataccgaat tgttgaggag tttctggatt ttcatccaca
     4381 tccattttag caattctgaa ttcttcttca tcatattctt gttccaactg atccaaaatc
     4441 ggtgcttgca tacgacatgg accacaccaa gttgcccaaa aatcaatcaa tacaagtcct
     4501 ttatctgttt cttcattaaa tgtcttatct gtaattactt gtgacatatc aaagacctcc
     4561 atttttaaaa tctatgacta gtatagcatt tctatacttg gatagcgaac catctgctta
     4621 cctttagtaa gctttactta aatttaacaa ttgtcgcacc atttccgcct tggtttgccg
     4681 gtgcaaactc aaagcttttg acgctccgat ggtttttaag ataatcagta attcccttgc
     4741 gaagtgcgcc tgttcctttt ccgtggacaa tagtcacttg aggataacca gctagaatgg
     4801 cagaatccaa atattgatct acctcactta gcgcttcttc gtatcgtttt cctcgaaggt
     4861 ccaattgatt cggcacgtga ctactagatt cagatcgtac agcatggata actcgttgtg
     4921 tcggctcttt ttgaggagca acaggcgtca gctcgtcttc ggacacattc atttttaata
     4981 taccgatttc tacttgccac tgattcccgt ttttacgtaa aagcgtccct ctttggccgt
     5041 aagtcgtaac caacacttcg tctcccggtt tcaacgtttt ttgttctttc gccttcttca
     5101 atactttatt tttcttcaaa gtttcttctt gatgtagatt tgctaattgg gtctttgcat
     5161 cgatcaactg atgctctttc acatttcctt gcccaatctg ctgttgcatt ttacgaatat
     5221 cagcaatgat tttttctgct ttttcttccg cctctgacac gacttcattt gcttttttct
     5281 ttgctttttc catttctttt tcacgttctt caaagaaata gctgtaagct tctttcaaat
     5341 catcgtgaag ttcttgtgcc tcagaaacaa aatgacgcat ttccagatat tcggtctctg
     5401 ccattttccg gcgattctct aaatctgtga tcatttcgtt taaatcttgg ctctcatcat
     5461 tcatcaactg cttcgcttca tcaataactt ctgtatcaag acctaatcgg ctagagattt
     5521 caaacgcatt gcttcgtcca ggaacgccaa tcagcaaacg atacgttgga cttaatgtat
     5581 ccacatcaaa ctccatgctg gcattaatcg tatttgcccg attatatcca taaactttca
     5641 attcaggata atgcgttgtt gccatcacat aagcagactt tttaccaaga tcatctaaga
     5701 ttgcgattgc tagagcagca ccttcttgcg gatctgttcc tgcaccgagc tcgtcaaata
     5761 ataccagact tttttcattg acttttgata aaatatctac agtgttcgtc atatgagaag
     5821 aaaaggtcga tagactttgc tcaatcgact gttcgtctcc tatatccgca aatacttcct
     5881 caaaaatacc gatttgactt tcttcacctg caggaatcgg taatcctgct tgccccatca
     5941 gttgtaacag acctaaagtc ttcaacgtga tcgttttccc acctgtgtta ggtcctgtaa
     6001 tgacgatggc ttgatagtct tttccgattg tgatgtcatt agaaactact ttttcctgat
     6061 cgatcagtgg atgtcttgct tgtttcaaga cgacatgatt gtcttcactg attcccggca
     6121 cgatggcttt tacttcttta ccaaatcgtg cttttgcatt catcagatcc attttaccaa
     6181 tcacataagt attgtggatg atttcttgac gatggggaac aagttcagca gaaagttcac
     6241 ttaaaatacg ttgaatctca tttcgttcag cgatttggta ttgacgcaaa cgattattca
     6301 aatccaccac ttgttttggt tcaataaata aagtctgacc tgaagcactt tgatcgtgga
     6361 ctacaccgcc aaatacaccg cggtattctt gtttgactgg gataacgtag cggtcatttc
     6421 gcatggtgat gatcgcatca cttaaatact ttgcattttt cccacggacg atcccatcta
     6481 actgttcacg aacagcttgt tcacttcgcc gaatgttctg tcttatgctc tttaactcag
     6541 gagaagcgtc atcagtcacg cgtccgtctt catcgattgc ttcctttaaa cggcggctca
     6601 aaacagggat cgtgactaac tgatctgccc acatatacaa acgtgcaaat tcgatttcac
     6661 tgtcagaaag atcatcaatg aaacgattca attcgcttgt cgtagacaat actctggaaa
     6721 cttgtgcaag ctccactcca ttcaagtctg ctccgatctc aatccgtttc atatggggac
     6781 ggatattttc aattttaggt atagggatcc cgcctcgcag acgttgcact ttcaatccat
     6841 cctcagtttc ttgaagccag tttgtgatcg tttgtttctc actcactggt accaattctg
     6901 ccagttcttc ttttccttgt gcagtcacta caaattgtct gaccatctgt ttgacttttt
     6961 caaattccaa tgtttcaagg attcggttat tcattttatc accttatctt tcactatgaa
     7021 aaaagagctg tgccggacat acgctacgca gccttcttaa ataaactact ctgtacaaaa
     7081 aacgaaatat tttccagcac tattttcctt aattcaaaat attcgttacc caaagctcat
     7141 acatcttgtt ggtaagaaat ggcgttctct caacgataaa acgagcgaaa ctatgctcgc
     7201 cgaattgatt ttggaccaca tctaatggaa caaaggcaag taactgcaaa atcaaaaata
     7261 tagtaacgta agccatgatt agtgacagga tgcctcctgc aatccagtct acctgtctca
     7321 agatcggcac atacgtcaat ccatggacaa aaacccccac aaatcgggta agcagccagc
     7381 caattagcaa aataatcaga aaagcagttc ctgcataaaa agcttcatct aggtgaaagg
     7441 caaatacctg atcaaaaaat gctaactttg atgttggggt gacagctggg tatggaatgt
     7501 ataattccaa atgacttgct aatggtttgt aaaaatgctg ggcagcaata aatgacaata
     7561 catatccaat cgcgaatatg atttgcaaag caaacccgcg acgtgctcca gaaaaaaacg
     7621 caattagcaa taaaaataaa atcagcaaac tcagcatgct acgttccttt ctcttctaac
     7681 ttttttaaat ttttcttatt ttgataaaag gatcttaatt agaagatttt tgtttaattt
     7741 gtccttttcg tttctcgttt aaaatctgtt gtgcttccac atgattttta atggtatgat
     7801 ctgcttgacc attttctttc aatacttccc gagcttcgtt ctcgattgct tcaatgcgtt
     7861 tcacacgatt ttcaagctct gtaaatttga tcattttttt ctttaattct gctgtttctt
     7921 cttccagctc caatattctt tcttgtttct tcaattgatc cgaaagcgca ttgacagcca
     7981 tcaagattgc tgcttgttca ttatccattt gtggtgataa ttgtttcaat tctgctaact
     8041 gttcattgat cagcttcgtt acgatatcca tatgatgctt ggtttcccgt ccgataatag
     8101 tatatgtttg gttggcaatc accgctttgt agcgtgtttt ttcgtgcgcc atacgaatca
     8161 tttcctccta tttgatgaaa gttttattta gtgtaccatg aagttggttc tttcaccgtt
     8221 ctccacaaat atttttcaca tacttaacta ttatatgata aactttaaaa aaatgctatg
     8281 tctcgcaatc tcttagaaaa cgaaaagtcg tgagttgcgg tatagtttta tgtataaaaa
     8341 cgaggagaat tttatgagca acgtcattat caaagcaaca aacgaccaac ttagtcaaat
     8401 gaaagcatac tatcgctctt atttgttaaa taaacagatt ccttatacta gctttgctgc
     8461 aaaaaaagac gggacgacca ttaccgctta tacatccgga aaagtgatgt ttcaaggtac
     8521 gaatgcagca atagaagctg ataaatgggg ggaggcacag gataaacaag ccaagccagc
     8581 aaaaacagcc tcttctttgc caaaaaatat cagtcaatta tccgttttag gtagtgatga
     8641 ggtcggaaat ggcagctatt tcggtccgct aacagtatgt gctgcttatg tgaaacgtga
     8701 tcagcttgca gaattacgta aacttggtgt tagagattca aaagagttaa aagatagcca
     8761 aatcatccag cttgctgaca tcttaaaaaa aaccatcccc tataaactgc ttgtcttatc
     8821 tccagaaaaa tacaatgaga tccaaccaaa atacaatgcc gttcggatga aagtcgcttt
     8881 gcacaatcaa gcgatccatt tgctgctaaa aaaaatcgag ccagaaaaac cagaagcgat
     8941 tttgatcgat cagtttacgc cggaagcaaa ttttaaaaaa tatgcgcgtt tagaaaaaaa
     9001 tcaattacaa gaaaaactgt acttcgttac taaaggagaa caatatcatt tagctgttgc
     9061 tgctgcttct atcatcagtc gtgccagctt tttggaagag ttagacaaag catcagctga
     9121 agctggaatc acccttccgt caggagctgg aacgaaatct gaccaagtag cggcaaaact
     9181 attggagaaa ggcggcatgc ctatgcttga aaaatatgcc aaacttcatt ttgccaatac
     9241 agaaaaagct ttgaagctac taaaaaaata atggaactgt gacaaaagtc atgtcacagt
     9301 cccttgttcc gaataaacgg tgttcaatca tctgatcctc ggtattagct caacaaacgg
     9361 caaaatatga ggaactattt ttccgtttgt ttctctaata ctcagaacaa aacgttgatt
     9421 tcacaacctt tttcttaaac ggtgttctca tgccaactcc tcggtcacaa gtcacaatag
     9481 tcatcaaaca tgagaagctg ttttttgaaa ttgctccttg tgactcagag ttgaacggca
     9541 ggctcacgac ctcttcctta aacggtgttt aaaagcttgc atctgcgata ataagcccgg
     9601 ccaaacgaaa aatatgagga gctatttttg tttggctggg cttatttatt actgaaaata
     9661 agtggtcaat gcttgtgtca aacttgcagc aatctgctgc tgataactgt ctgttactaa
     9721 ttcctttaaa tcactatcat tattcatata acccatctcg attaaaatag aaggttgaag
     9781 attttctcga agaacttgat agttcccagt ggagattcct cttgaatcca gaggcagatc
     9841 agtgaaggag tcagcaattg tctgtgctaa tgtttcctct tgtccatagt aataatatgt
     9901 cgtgattcca gttgcttcat tcgcatattc cgtagagtct gcgtgtaaac tgataaagac
     9961 atctgccttt ttttcttcac tgtagtcgca tatatctcca agctgtacta attcatctgt
    10021 agtatgggtc aaataaaccg ttgctccagc atcctctagc gcctttttta cttttatggc
    10081 tgtacttaat gttatatctt tttcttctgt ttgttcatca ttgcttaatg ccccagtatc
    10141 atttccgcca tgcccaggat ctaatacaat cactgcatca gatagctctt ccggttttgt
    10201 ttgtatattc tttgtgactt tggttccatc ttcgagggct aaatatccag tatagccatc
    10261 atttgtcttg atctgataag cattttttgt ttcagaaaga aactccgcca gctctccgcc
    10321 gtttatagtt gcaagtgggg tagttagcga gtcatctgta tatagtgtac ggtcattttt
    10381 tccgattttg attaatgtct cttgttctgt atcatcaaat gattcctttg tctttgtaac
    10441 cgtactgttc gtactgctga ccgtttgtcc aactatcttt ttttgaccaa aaacatctat
    10501 ttgaaaacag ataataatga taatgattgc aaatacaaaa gctccaatta tttttttcat
    10561 tcttgactct cacctcgtag atccagacta agggccaaaa cttaaaatca gcttaagtaa
    10621 gcaaaaaagg ggctgtaaaa gatataaccg tagaaaataa cattactgct aaaatgattt
    10681 ttttcatttt tggcatatcc tgaaatctcc ttttgactat tcgtggtgat tcatctaccc
    10741 aataaatagt ttccttcatt ctacaaacaa ttaatttctt cgttattaaa ttaaaacact
    10801 tcctggaaca ccgacataat aaataaaaat ataccctgat aatcatgaga gaataacaaa
    10861 tttgtattta tgaaaaacta cctatataaa gaagcaagat ataacgatct tcgtcatatc
    10921 ttgcttcttc acctaactat tttctcccta tataacagct ttacgcttct cctaatacac
    10981 cattcaaata taaatcgatc aattgcgcca catttagcgg ttcttccaca ggtcttttcg
    11041 accgagtgat gtaaggtgct aacactaaga aaaaatgcct tgctgcgatt tcttttgaaa
    11101 tcccatttct tagcacagat tggtttgctg aaaagatttc ttcaaaaggt tcacgataat
    11161 ttttcttcca tagaaagaaa agatgatctc ttgtttcctt tgaaagattg ctttcaagat
    11221 cgtgaagcat cagaaacaaa tcgaccagat gtgtctcaat caaaaactgc gccatttttg
    11281 tcaaggttac ttgaaatccc gtttcgcttg tttgaacgat ttctctcaaa gcttttccaa
    11341 catcttttaa gtggttttct aaaactgccg tatatagttt ttctttatca ccgaaataat
    11401 gatagagatt gggctgggtg atcccaactt tttgtgcgat ttctcttgtt gaagtgccga
    11461 gatatccctt ggacataaac aagtcagtcg cttctttcaa aattttttgc tgtgtatctt
    11521 tgttgtatcg atggaccatt tttccacctg ctttttggtt attctttttc tttcaataca
    11581 ccatcttcca tcataaatat tctatcgcat ttgtcaatca gccgtgtatc gtgagtaacc
    11641 atgatcgttg ctttattttt gtcttttgat tcttttgcca agatatcgac tacttctcgt
    11701 gccttttctg aatccaaact agcagttggt tcatctgcta gaataatgga cggatcattg
    11761 tacaatgcac gagcgattgc gaccctttgc cgttctccac cagatagttc ttctggatac
    11821 ttgttgatca acttatccac gccaagctgc tcaaataatt cgtttgcttc tttttgacga
    11881 tgttcatgtt taatcttatc tactaataat aactgctttt ttactgtcag aaaaggaacc
    11941 agattggaag cttgcaaaat aaagccaatt tctttaaaac gtagtttaac gcgttcgctt
    12001 tctttttgat tactgaattc ttgatcattg ataaatacag agccactgct aggcgtctgc
    12061 aatccgcctg ccaaagtcaa aaaagtactt ttacctgaac cagaaggacc gatgattgca
    12121 acaaattccc ctttatcgat actcatggtt gtttctttca atgctttaac tacttggtcg
    12181 ccatcttgga aacttttttc tacttcttta aattctattg ctttcatgtt cttcctcctt
    12241 cttatccaat tgccttcaac ggatcgattt ttacgattgt ccgaacagaa aatagtgcgc
    12301 ctaacacagc tactactaac atcaacaagc taatgacgcc aaaaaacagc caattgcttt
    12361 ggaatggaac cgcatcaggt agaacaaggg atgttcccac tgttcctaat agaccaagaa
    12421 gaataccaac aaatgataaa atgaatgttt gtgccactac agaacgcgca ataaatcctc
    12481 ctgtgatccc ttgtgctttc attacaccaa agatattaat cttttgcatc gtcagtacat
    12541 aaatgaaaat cccgatcaca atagccgcaa taacgatcaa aaatccgatc ataaatccaa
    12601 aagtcaatac ttgcgcatta tatccaggta attcattgat gaatttagaa attttgatct
    12661 gttctaaatc ttcaggtaaa ttactgattt tacctctgac aactattgcg ttaatccgtg
    12721 cattttcact tgtatcttct ttttcgaagc ggatttcttg gtaagcattg atggttgtat
    12781 agagaactgg tgagacattg aatttggcat gatccgtaaa gccagttagt ttaaacgtct
    12841 tgtcactacc agataactta actgtatctc ctatggctag accatattct tctttcaaac
    12901 taatatcgcc tactgcctca tcatcgttat caaaagcttt tccttcaaca agattgggca
    12961 tgatgaattg atttttatcg ataccgaaga agctaacatt gatttttcct tcttctgtac
    13021 tgtctttact agtcacgact ccaggtgttt gggcaagata cgccactttc ccaccttcga
    13081 cttcttctgc tatcttctta gtaatcatgg acatccctag atttgaattt gcatctttcg
    13141 aaagcacaat cgaatctgcc tcccacttgt cgactgctgt tcgattatct tgagctaagc
    13201 cataagctaa cccagtcaaa aaaaatacca ggtaggcgat caaaaacatc acccctgcaa
    13261 ctaacgcata acgtaacttt gaatgcataa tttcatttaa tgctaaaaac atttaactcc
    13321 tcctcctgaa ataatcattt tatacaacaa ttttatcact tgataaattt ttggcgaata
    13381 ttatgctcaa aaaattcttc cgctatttca ttatatgaat tttttattct cgattgatta
    13441 atatttatta tatccccaca caataaaaga ccgtgatatt tctcacagtc ttttatctac
    13501 tctattgcta tttgctctct tagctagtac tttaaaagct aactcattga ggtagtgaaa
    13561 acgagaggtg tattcagaca gaaatccaaa atcctcaact gctagctgtg gaatcgtagt
    13621 tttgtgtttc acgatatccg caagcaggtt caaatctgat aataagaatt cttggcttat
    13681 cagctgtccg ccaattattt ctaatgtatc cggatggtag atcagctttg catttactgt
    13741 tttgttttct tcgtcgtatt gtggaaatag atcaaattct cctgaacacg aaccggtatt
    13801 ttgttccaag aatggcgctt catcttctgt cagccctaca gttcctaaaa agtagtctat
    13861 aatgactgct gctgttatct tttgtctgag gtcgtaagcg gttggctgac cactcaaagt
    13921 tagcgctact gctcttgcca tatgtacagc tcgagtaact aaaggtaaaa agagatgccg
    13981 tttatttttg tcaaaactga ctgggacgat atctccaatc gcatagatgt ccggatcact
    14041 tgtctgcaga tattcattga ctatcacagt ctcatcctca tacaaagcca ctttttcttt
    14101 taacaatgtc gtattaggac gagtttgggc agaaaaaatg accatatctg cctttattgt
    14161 ttgagacaga agttgtacgg atactacttt ctcttctgat tttttccaat cgatcaagaa
    14221 ttcatttgga taaaaattta tttgcttagt ttgcagctga ttttgaaaac tgttgatcat
    14281 atccttatcg aaatatcttg ataaaacatt cggcatataa tcaactagat gaataatgta
    14341 tcctttattt ctcaaagcat ctgctaattc caatccaatg taaccagcac cgaagatcac
    14401 tacttctttt gcttctttta aagcggcaag tacttgctta gattgcacta gatttttata
    14461 ggtgtagaca ttttcagctt caatttgttt atacaaggtg acaggagagg aacctgtcgc
    14521 tacgattaat ttatcatagg ttatggtctc gttttttcct tctttggatg cgaggatgag
    14581 ctgatgtttg tctggatcaa gttcagttac tctccattcg gtgatcagtt ttgccccact
    14641 ttcttgaaga gtccgcatgt tatgggcaac ttcagctaaa ctacgtatct ttccttttaa
    14701 ataaaggttg attccatttg aaacatagcc tagtccatct ttatgatctt catcgatcag
    14761 taaaacttct gctttcggtt gaagttttct tagatttagg gcagctgtga tgccttcatg
    14821 ggaagcacca atgatcacga ttttcatgtt ttattcatcc ttaaattcaa tttttctaaa
    14881 taacattctt ttaaaaattg gttcaaatag gggaaatcat aattatattc gttgctagtg
    14941 aatacaaaaa ctttagcgga cttctgtgac gggtactctt tcaattggct agtcaaaatg
    15001 atatcatact gcttttcttt ctcgtattcg actaaatcat atttgatcgt attcagcaat
    15061 tgttcctgga tgatttcttt cacgatatcc gccattagcc tttcataagg aagatcataa
    15121 gcaatttgca gacgtaattc taactgcttg atcagcgtcc gtaaaataga tgcatagcga
    15181 gcaaataatt caaagcgggc attttcctcg tctacttcag taaaatcaac aatagcaaat
    15241 gtttgtctga ccaatctttc acataaaacc aggaattgtt tatcttctac ttcagttaac
    15301 cgctgtttta atccttcttg gccaaaaata ttcatccatc ctctaaaata ggcaaaatga
    15361 gcgtgatttt gcatcactgt caacgcaatc ttttgcgaaa tctcttctgg aaaaacataa
    15421 aaagagaaat attcttctat cgattgttta aacgttcgat tcaattgagc tactacgtcg
    15481 tctttgtatt tttcgcttat caaaaactcc tggacatgcg agttatgtgt atctaatgta
    15541 aagttactga tcatgaaaag ataaaaaacc aacgtttcat tttgattcca aggaaaggca
    15601 tattgaaaga agtaacgggt caaaaatgta tggaggtcca ataaaaattg tgtcgtttga
    15661 aacaatccaa caggaagatt gctataatct ggtttggatt ttttcgtaca gcgcattcgc
    15721 ttcttcataa tggaaagcca tatttcaaat ttcaatcgtc cttcttctgt aatagcaaaa
    15781 ctaaactctt tttgcagcag atctaaaaaa gtgtttcctg ataaatcact gtattctttt
    15841 ttgttttcaa gatagggacg tccaaaccaa aataattgga aataaaaata acgaatttga
    15901 ctttctggac cggtcagccg cccgcttttt attttcagat gaaactcatt taaagaatca
    15961 tttaattcct tcactctgcg agacaaagta gccgaactga tattaagttc catgacaagt
    16021 ttctgccgat caaatgagcc attttccaac agataaagaa gtatctgata ttttgtagaa
    16081 tagcgcaagt aacaatgata ataaacatcg actgctgcta tatttccatg tctgattaat
    16141 aaaatattcc cttcttggat aacaatttcc aattgtttga tattttccaa gttagcttgt
    16201 aaataaaaca gatcttcttt caaggtagcc cgatcaactg ataagcgggt aagtacgtta
    16261 ttaacggaaa cctctccttt ttcctctaat aaaagttgga aaagtttgat ttgccgttgt
    16321 tcattttttt caagcaaact gaaaagttcc aagtccactc cttcaagcat ttctgatttt
    16381 aacagcagaa gtttccttct catccttgaa aaagaggatc tgtgacagtt atcacagatc
    16441 catacaaatt ctaatcactt agctggttgg cccagctgtc agttcccatg tgaccatgcc
    16501 ttgatacgtt gcgccaggtt gagcattcgt actgctattt acctctaaga gtaaaccatc
    16561 ggtatcggga aagtcaacga gaacctctcc tttagctgtt ccatcactct cataaatcaa
    16621 cgtgttttga ccttgctgaa tcaattgctt tgatccagaa tgattgaagt ataattttgt
    16681 tccgcttaat tcttgacctt gatcattctt aaataaagaa gtcaatgtac ctgtcagttg
    16741 ccaaggagac gtgcttggaa gccgagtatc ccgcacatca aaagaaaaat cgccttttga
    16801 ttcataataa cttgtcttca acggtttggg gagatcgtta ccaaatgtaa aatcatctgg
    16861 tacattaact aatgttaatt cttgtttggg gacaagaatt gttttagcag atgtttggac
    16921 aactgggtta ggaagcttag caccatctgc taatgtaata ttgctggaaa gtacagctgc
    16981 ctttgtcagt atgctgacgg attgtaaaga agtatctaca gtatttaatt gatagttgta
    17041 agtaagatga atggtatctc ctattttgct caatagatct gctgggacag aaactccttg
    17101 ttgaatgccg gtatctgcga tagttgtacc atttaatttt aaggaaccat tgatataagt
    17161 agtattttca ggaagtgaga tcgtgatttt tttgtcggtt atcgcatccc cagattttgt
    17221 taacgtgaat gtcacatttt cttgaatcgt atctcctgat agagcagaca ggggatctcc
    17281 tgtctcaggt tgtctgctaa ggttttttag ctgactatct gcactcacag atggaaaatt
    17341 agccactgtt atttgaggaa gagtggtgat tccagcttgt tcagactgcc atttgtacgc
    17401 aaaagacgga tacatgttag taagtacatc gtccaccaag cgataatcaa aataatattg
    17461 ccctgaagcc aatgcaggta gtgtagaagt agctgtattt acacccccgt tatttgattg
    17521 gatcgtttgg aaaagttgat ctgtccatcc tgtcgctaat ttttgggttc ctttcgaata
    17581 aagcctgtaa tggatcgaac ccttttttag ttcagttgct gaccaattgg ccgtgacttg
    17641 gtacgatgca gaagaaagga gctttcccca ctgactatcg gctgttaagt ttgttggtaa
    17701 aaccgttccg tctagcttgt actgctccaa tacctttaaa gcaacaggag aaagcaatcc
    17761 gccttgcgca tctttcccac tgtacacgat attggctgtc tgattcaatc cacttgtgct
    17821 tgctggagta tgagtcagta ttgctccttg tgtaacgttt tttcctgtaa aatagttgac
    17881 tacgcgatca aagcgactaa atggcaactg atcaaaacgt ccttcagtca gccccatcgt
    17941 caatgtccga ctattggtag agcctggaag gttctggtta tttccatcca cgtattctgt
    18001 ggtatcagca aatgtattat attttcctgt tgaatcactt ggtgagatgt aactattcca
    18061 cgtatttatc tctgcacttg cagaaaatgc atatgctcct ggattcaagt ttccgccagt
    18121 taatttagga taaaatctac ctgcagctgg agctaaaatc gaattgaaca tacgatcttg
    18181 gataatatta ttcgaatcat aaaaatcaac agaaaaaacg gcctttttag acatagaagg
    18241 ttgtaattta cccttggtca aaattttgtt tggactcgta tcatatttat ctccgttttc
    18301 tgtcatcttc gcgacattag ctgtaacatc aaattgaagc gggatatcgt ccacgttgaa
    18361 ttcgcctcgc gtaataatag caataatatc tgtcaataga gtatttgtat cccacgaccc
    18421 catcccaaaa cgcaaggtat ggcttcctgc tgataataaa cttccgctag taaccttgat
    18481 gggggcaccg gcaatataca agtaggcgtt gttccagtca attgctttta atacatcact
    18541 gcttgataca tgttccggca aattgatgat cgccacaaat gcaaatcctt gtgcagtgat
    18601 attcaccaaa tcaagtacat ttgttagttt cacatggctt tcccaatgaa aacggaccgt
    18661 gccacctttc caaggatcat ttgaaccatc gttcaaataa gtagcagtca atttaggcaa
    18721 ttggttagcc ccagttagtt ggacaagctt cccgccattg actgcactac gtccaccatt
    18781 tggaggtacg atattttcat acgtgtatgg atcataagaa atggcaggag gcgttgctcc
    18841 aacagcttct gccgaaggtg aaaagataaa tatacttaaa acaaaaagcg aagtaaaaaa
    18901 gaaggagcga aaaaatgaaa atttattttg tagcaacatg ttcatcctcc ttttcctttc
    18961 ttctccataa caacaaccac gcaagaagcc ataattctat accaatgatc aaataaagaa
    19021 aacggatctt ttgttcattc gtctccggga atacgccatg ttgattagat tctttataaa
    19081 tcggtagctg tgtctgctgt tttgatgcgc cattgactga accacttgga tgcggaattg
    19141 gacttagatc ataaaatgta atcgtacctg aacttttggt atctgcttgg actggctctt
    19201 gccagctgaa gaacagcaaa cttgcaataa tccctcccaa taagatcgat gttttcaacg
    19261 aaatcacctc atcatgttgt ttctcctttt ttctttttct tgtggaccca caaaaacagc
    19321 aaaatcaacc ataacactag aactgcagca accaatcctg cataaagcca tgggaaagat
    19381 tgttcttgta ctgcaaaaac ggaattttca tttacttttt gtgctttttc ttttttgatc
    19441 gtaaagtttt gtgtaaatcg ccattcatta ctgtcgcctg attttgctat gatttctgcg
    19501 cggtaatcgc cggcttttgc tttatcacca gctaaatcaa gattcaagtg aaaattcgaa
    19561 tttggtgcaa ctaaaaatgc attagacgta ttttcaatat aaagctgatt ttcataaaag
    19621 atcttgctcg taaatatcaa attgctgata atcctaggct gaggattttg gaaacgaagc
    19681 tgaacagttg gctttgcgtt gacctcgtcc aaatctcctg ataaaagttt cagatccggt
    19741 tctatcgtct cttgactttc ttgtaaaacg actgcgagat tataagcaat acgatttttg
    19801 atttgtgctc cttctgtagc attttctgtt tcagcttctt ctttaggtga aacatgaata
    19861 cccgctaata cacgtccctt aaagcttttt gctgggattt tcacttctat aggtacagtt
    19921 gtttctgcat gtgctgatac attgatttcc tttttcgcaa tgagcaaaac gtcacggaca
    19981 tcaaacggca atgtctcatc taacgttggg tttttttgac tataatcgat cgttccacca
    20041 tcactcgtta ccgccggatt gacagagacc tcaaatgttc gttcttcatc tgaagaattg
    20101 ctgataatta cttctagtgt ctgattttgt tccggtgcca ctaataaatc aaaataaccg
    20161 attgctttat ctatttggtt ctctggaaat accggtttga ttccaaagtc tcctgctgaa
    20221 tcctttgctt ggctgattga cggaaaaatg aaaaatagac ttaaaataga aaaaaacgaa
    20281 acaaacagtc gtttactttg attcattgtc caccacatcc tttcaacaac aactgcggta
    20341 aaaaatcctt cacctatagt attttcaccg cagtttgttg aatttatttc atactctatg
    20401 ctactgggcc agccacgagg ttccaagtca aatcagccgt gaatgttcca gctgctgctt
    20461 tcggcacttt cagtgtgata tttgctggat cataaacaga aagccatgtt cccagacctt
    20521 gatcttttgc tgctgtaaat attggtgtcg ctgcacttgc tttactcaac ccagttacag
    20581 tttgactagt tggtccagtg gattcgttat ctgacgtact tttaacagtg gatgttccaa
    20641 aatctaattc agcgccttgc aaaacttgtg aaccgtttac aaaatctgaa accgaaactg
    20701 tcacgttcca cccttgtgct tggccatctg cacctacacc tcgacgatct gatacttgga
    20761 gataaggtgt atcgtttttt gaagcgtcta cttgatacgt ttttgttccg ctttcaatct
    20821 cgtgtgtacc aaatggcaat tcgggaacta catccagtgt taaaggacct gtctggccag
    20881 ttgctggatt ttccggatct gctggctcta ctggttttga aggatcagta gggttaagag
    20941 gctccacggt atttgttggt tcagtaaagg taatatctcc ttttgaagtc gcttgaccct
    21001 cagcaaaagc tggtgttgcc aagccgccta gaagaagtgt tgaaagtaat aatgtcccaa
    21061 tcatgttctt tttcataata aatcactcct ttttcttagt gacctaagta tagtagagac
    21121 agtatgcaaa aaatcctcat gaattcatcc gcttacttga aattatgacc ctttttgtat
    21181 aaaagagaaa aaaacaataa aatcgtttac atgaatattc ttcctttttt attaagtttc
    21241 gtttgtcaaa ttaagttaga caaatcatct tttgtgacgt aactcaaaaa tacttccatt
    21301 ggtgtccgat agtgtaatga ttttctagga atatgatttc gtttcgatgc caccgattgg
    21361 ataaaggttt cagatacacc attaaaatcc atagctttat acaaaccatc tttacgtaat
    21421 aagccatttg aatgttcatt caatcctcgt tgagaaggtg ttcctggatc cgcaaaataa
    21481 attgaaatat cgttggtatt actaatcttt ttccaatttg agaattcttt tccacaatca
    21541 aatgtgatgg atttaaataa gttttttggg acacaacgaa accattgatt cagcctattt
    21601 tcaatgtctt tagcctgtct gccttcgggt ttcaaggtaa tgatgacttt agatagccgt
    21661 tcaactaatg tgatgaccgc gcttttatgt tttccaccca cgatagtgtc accttccagg
    21721 tgaccaaatt catgatcaaa ctgggtatag tctgtctttc tgtctcggat tgaccggcga
    21781 aacccttgtt ttcctctttt ttctttatga ccgtttggtt ttctttttcc cttcattggt
    21841 aaatcgtgag tgtcaaaggt tttatcttta aacattcgat acagtgtgcg taccgaacaa
    21901 gcaataggaa actctgctcg accaataagg acatcaggag cccacccttg aacaaccttt
    21961 ttttgaatgt attcctgttg ctcatcaggt aaaacaatcg gatgtcttcc gcagttacgt
    22021 ttgtttttct tatattgttt atagtagctc attgcgcttc caccagattt aagaaaacga
    22081 tagaccttat aaatagtttg tcgagaacgt tgtaacagat tggctacttt tgaaacaggt
    22141 tgtgattgat tgaaataagc ttctatcatt acgagttcag ttggtgtaag atggttatag
    22201 gtcatttgta ctcactcctt ataattcttt cgtcggaact attttgagtg tagcacaaat
    22261 gacttttttt agttgtctag cttaatttta caatcggcga agtatgaaaa aatatttcaa
    22321 ttttattttt tcgtttcaaa ttattctatt agtgacatcc ttgaactata aaaataggag
    22381 tacggcatca tctgccgcac tcctattttt ttcgtcattt gtttcttaaa taaaacgatt
    22441 ctcacgcaat tttatttttt tgatatcttc tactgtttgt gttccagcta attgcatgac
    22501 catttccaat tctgttttaa agaaatcaaa cacttgatgg actcctgtac ttcctcctaa
    22561 agacaatcca taaataacgg gtctgccaat agcaacaaga tctgcacctg atgcgattgc
    22621 tttgaaaaca tgctgtccgc gtcgtacacc gctgtcaaac acgattggta cacgtttatc
    22681 tactgcttct gccacatatt gcaacgaatc aaacgctgct ggaccaccat ctagttgacg
    22741 gccaccatga ttagataccc agattccgcc agcaccagat tctaatgaac gatagacatc
    22801 ttcttctgac tggacacctt tcacatagac tggcaaatca gaatgagcag caataaattc
    22861 tacatctttt ggactaagct tctgtttgga agatttgtac acagcatcca tcgtttggcc
    22921 tactcctgat tgataagcct gaacgatcgg cattggcagt gggaaggtaa acccatttct
    22981 gcggtctgtt tcacggtttc cgccaactgt tgcatccgct gtcaatacga ttgcttttgc
    23041 tccattacgc ttggccactt caagaatatc caaattgatc ccatcatctt tgctcatata
    23101 aaattgaaac cattgcggtg ctttctcccc gcctgcttct cttatctctt ctaatgtaca
    23161 agaagcgtaa gaacttgctg tatagatcgt cccaaaatct gctacacctt ttgcagaagc
    23221 tttctccgcc ttcacatgag ctaagccatg agcagcaact ggtgccatga tgattggtgc
    23281 agtcagcgtt tctttgtcaa aatgtgtagt tgtatcgggt aattctacat ctcttaatac
    23341 atgaggaata atcagctgat gattgaatgc ccgttcattt tcttgataag taaataagtc
    23401 gccagcacca ctgctgatgt aaccataacc accagctgga atcacttgtg aagcggctat
    23461 ttctaaatct tccatattga taaaatccac tattccttca tttgtacctg cttgatacgt
    23521 tttttccatt tcaatgcctt ccctctataa aaaattgaat gaataatatg atagcgttta
    23581 cacactttat attactcttc cgtattgata caggcaaata atctgtttac atttcagtca
    23641 ttccagttgt tactactctg aaagaaatgt ttagaacaca aatagataaa aaacgatggg
    23701 attttgacaa aagtcgtgtc aaagtccctt ttacgaaata ctgtggatgc ctattgaaat
    23761 aagtgcgggt tgtttctttg tatttcaatc gtttcttgcc attcttcgtt taattttgga
    23821 ttaccgtttt cttccttgaa cgcataaggt cccaatccaa aaagctcagt tagtatttga
    23881 ttcacgattc catatagctc ggtgtgatcg gacacatgat tattgaagat catctcatta
    23941 tcaccacgtt tgaatctcac attcgggctc tcatgatgaa cctctatgca gttttcccct
    24001 ttttttaact gacggatttc tgaaaacatc tcataaatat cttctccaat aaaaccttca
    24061 gcaagcgcca gactgtccac aagaccgcct actgtcaact cagagattat cgctttgttg
    24121 gcaccaagct ttaatgtata gcttgttgct tctactacgc cgcatgcatt gcaaccacct
    24181 tctgatagtt cgctttttct gatcaattga acttctttca ttttctgttc ctcctcttag
    24241 ttttctactt atgtattttt tatcacctga cgtaagtgta ttttatcgtc acgatcacgt
    24301 gatttattct tcagagattc tacctgaata attcatactt ttaattaaaa ccatgtgaaa
    24361 ctgcctcacg gcagcttaca attacttttt catcttcacc cgttaagtga tgaaaaaaga
    24421 acccctttct gctatggtta aagtgactaa accaaccaaa agaaaggagt tcttttcatg
    24481 actagcttac acaaaaacca agtaaaattc aattcaaata tcactatttc tcatacaggt
    24541 ggtcaattat cgagtgattc gggtctcgtc ctagtgaaag agctgatgaa catctttggt
    24601 ttttctgaac tggctaagca acacattcac attgaagacg aacgagcgta ttttactcat
    24661 gacaacttat ccatacttga gcagttcatt atgcaattaa ttgctggtta ctcagctgat
    24721 tctgcagcta atctcctgag acaagatcct gtgtttaaag ctgttttaga taggaaagaa
    24781 ctcgcttctc aatcttcact ttcccgattt ttagatcgac tatctgagga gaatatccat
    24841 gaacttcaag ctttgaacca agaacttatc gataaagcac gcctcatccg taatgatacg
    24901 gaattaatca ttgatttaga ttcgacccat tcagatacat ttggtcacca agaacaaacg
    24961 aattataata cacactacca aacctatggt taccatccat tggtagcttt tgacggattg
    25021 actggtgact tcttaaaagc tgaactacgt tcaggaaatc aatatacatc aaaaggcgta
    25081 aaggagtttc tcacaccttt attagagcac tataatcact ctctaccaaa cactgacatc
    25141 ttggttcgtg gagacagcgg gttcgctaca cctggtgtgt atgattcgtg tgaatcaaaa
    25201 aagagtcatt atgttattcg actgaagaat aatcgtagac taggtcaaat agctgagaaa
    25261 tcagtacttt atggcgataa tcaaaagtgg gaagaacgag aagttcagta cttctcgacc
    25321 acttatcaag cacaatcctg gtcacaaagt cgtcgcgtgt gtatacgctc aacacgtgaa
    25381 gcgggcgaac tactctttcg acatgaattt atcgtgacga atctatcaga aaatgtttct
    25441 cctgatacca tcttttctat ctatgccaaa cgtggcacaa tggagaactt cattaaagaa
    25501 gcgaaagctg gcttttactt tgacaaaaca gacagtcctc gctttttgga gaatcatgtc
    25561 cgaatgatgc tcagcttgat agcttacaac ttagtcaact tcttaagaac gattggcttt
    25621 gaagaagtcc aaaagggaat gaccattcat tctattcgat tgaagtttct gaaagttgct
    25681 gggaaattag tccaaacggg tagacgagtc tatctcaaat tatctagtta tcatgtgtat
    25741 cagaatgaat tctacagggt ctttgctcgc ctgaggcgag ccagtcaatg gatctaatcc
    25801 aacaatctaa cgattttttt actgggaagt tatccaggga gaagtatgct caaaatgcct
    25861 tacacccaaa atttattcct caatattttt gaacagtgaa tgtttgaagt aactttttag
    25921 tcaaaatcag ttgataggga tattagtgat acatatttca ataaaatgtg ccaaagaatt
    25981 aaagctatga attattcagg acgagaatct tacttctaaa ttctcattta caagcagcta
    26041 aaaaaagagt atgataaata aaaatcgtgt taaggagtgt cgtacgatga gcgaagcaaa
    26101 ccaagaacgg tctggacaag aaatagaact aatcaaagaa cgtattcttg ctgccttgga
    26161 aatggtgatt gatcctgaat taggaatcga catcgtcaat ttaggcttga tttatgatat
    26221 agaattcaat cctgaaaatg gagaaacagt cattaagatg actctaacaa ctatgggctg
    26281 tccattagca gatattttaa cagaatctat ccatgacgca ttaaaagaag tgccagaagt
    26341 aacgaaagca gaagtcaaac tagtctggta tccagcatgg acgacagata agatgagccg
    26401 ttatgcacgg attgcgttgg ggatacgata gaaatcttgg ctgactagct atgagaagca
    26461 aataggaaaa acaaaaaagc cttttatcct ttctagaaga aaagagataa aaggtttctt
    26521 tgtttataaa attttgtctg ctatattata agttaattaa ataaatgtgt tctattgata
    26581 aaggacattc aaatcccttt ccttttggaa tccttctttt ttacatcagt ttgattttcg
    26641 tatagtattc tttctcatct tcacgataaa tcgcagcccc atttttttgt tgtgctttaa
    26701 gactgccaag atttttttta tttacagaca aaagtgcctc atcaatgccg attttttttg
    26761 cctcttgtag tactaatgct aatccttgtg tggcatatcc ttttttacga taattagggg
    26821 agatgccgta accaatatgg ccagcgccgt ttcttaatgt ttcgttcagt ttatggcgca
    26881 aattaaaaat tcctacatac tcatgttcat cactttctaa aataaagacg gttgcgggaa
    26941 cgtaactctc aggaagatcg attcctaaag acgttttttc gcattttttt acataatcta
    27001 aaaattcagc aaatgtatag ccataagcat tgttgataaa tccactctca tttggtccaa
    27061 agctagtttg tagctgataa gctttcttta aatctgcttc ccatagtttg atcagtttca
    27121 tcgtattcac ctctttaagt aaccttattc atttgttttc tactatagca ctattgttgt
    27181 aaatagccat gcctattttt atttattgtg tcatatcgta ttaagaatca tcgaacaata
    27241 caaatatgaa ttacaataaa aaaattattt gaagttcgtt cattcgttac ttatgatctc
    27301 tagaagaaag aaaacgtgaa ttacttcagc gtctagaatt ccagaaggat agtaatcaac
    27361 agaagaaata atcaatatcc aaatgaaaga agatagctga atcgagaaaa agaggaaaaa
    27421 agaaatgaag aaaaaaataa aatagttact aatcccagag tcgttcgttt aacattgaaa
    27481 tcgatcatat ggataaaaca gattttttca ccaaccctca atagatgatg gagttgctcg
    27541 gatgaattat ttgtgtagat ggcataaggg agggcgtaca gaaatgaaaa agaacaaaaa
    27601 tatgttcttg acaaaaaaca catgtaatcc taaactaaaa gatgtgatgc ggctatggcg
    27661 gaattggcag acgcgctagc ttcaggcgct agtgagggtg acctcgtgga agttcgagtc
    27721 ttcttagccg cataatttgt ttttttataa agagaaggta cacctggagc gatgagaaag
    27781 aaaataacat attatctaaa agaatatagg aaaaatttta aacaacggaa ccagattcaa
    27841 ataaatcaaa aaaaatttgt gtgaaggtgg gacagtttaa aacctaatag attgattcaa
    27901 atagtagatt tatagattct gatacaagaa atactggcaa acgatagtta aggaataaag
    27961 actaaactta aactcaaaaa gaggtcatgc gaaagaataa atcgcatgac ctcttactat
    28021 attttgaatg tgtttaaacg ttcaaataat gaatcttagt accaaccgtt tgcttcccag
    28081 aatgctttcg ccgcttccca agaaccataa cgtgaagtaa catattgttc tgctactctt
    28141 tcttggtttg cagcagagta gtcaccattt aagtaagaag aatctaattg gtaacggccg
    28201 atgtaacgtc cgttcgttgc tgtgtaagaa ccgcttgatt ctttttgtgc gatccattct
    28261 tttgcagagc ttgtacttgc tggagtagca gcagtttctg ttacagcagg agcaggttgt
    28321 gtttcagcaa caggcgctgc ttgtgtttcc gtttgaaccg tttcttgtac aacaggttgt
    28381 tcagcttgaa caggttcagc ttggactggt gcttcttgaa cggctgcttg aggttcagca
    28441 gctgcttgtg cagagccttc tgttgggatt gttaattgtt gtcctgagta gatcaggtta
    28501 atatcagaaa ttgaatttga ttctgcaatg gcattgatca aggagttatc accaacgtat
    28561 ttttgagaaa tcgttgatag agtatcacct gattgaacgg tataagcttc gtccgcatga
    28621 gcagtcgttc ccatgaagaa tgcagcgccg gcagccaaag ttgttccaaa aagtaaagtt
    28681 ttaagtgatg tcatgaaaaa tctcctttat tcgttaaaaa gttttatttg ttgtgttcaa
    28741 cgaggaatac tttaacacgt gaaaatattt cataggtaaa agaagcatga caatttatga
    28801 caaatccacg ttttttgtaa cagaatgatg ttcgctcgat gtgaaaaatg atgaggaatt
    28861 tcacataatg cagaaataat gggattagaa caggtgttct gatgagaaaa gaaatgtata
    28921 aataacttga aagaaattgc ttttgttcat cattttattt acatattgcg tatatttttt
    28981 gtatttttgc ttgagatgtt acaataaaag aaaaaggagg cggttccaat gagtaaaaga
    29041 taccagaaca tcatggtagc agttgacgga tcaagacaat cgatcaaagc gttcgctgaa
    29101 gcccttgact tagcaaagga caatgaagcc cacttattta tcgtgtcaat catcaacaaa
    29161 gttgagctga cccatagtgc ctatgccttt tcaaaaatct atgcagatga aaaacaaaga
    29221 acagaagtag ccatgctgaa aaaaatcaat gatgccaaag agtacggcat tgctgagatc
    29281 cacgcaatag ttgaaactgg agatccaaga aatttgattg ccaatgtcat ccctggacaa
    29341 gaaaatattg atttgatcgt tatgggagcg acaggaaaag gtgctataca acaagcatta
    29401 gttggatcga ccgcttctta tgtcgtatca catgcccctt gcagtgtgtt ggtcgtgaag
    29461 taatgagata agggaggaat agaaaatggt tcgtcctcaa gaagtccgtg cgccaaaaga
    29521 aaaaatagaa attttagcaa ttattgaaga cggcacacaa acaaagaagg gatactcgat
    29581 cgctttagta aagtggaaag gaaaaaaagg tgttgctctg agatgggatg gagataacca
    29641 acaagataaa ggattccctg tcacagctaa cggttatcat ccagcgtggt ttgttctgcc
    29701 tgataaattt atggaattat acagctatga ttatgcaaga gccgcagcat caatcaaagc
    29761 attagataaa ttaacagaag agcagaatga aatggaggaa aaataaaaaa gacgaaccaa
    29821 tcagccttta gaaaactgat tagttcgagg agtaaaaatg aaaaaaggtt atttgggatg
    29881 aaactagaat aatgcgtata acttaaataa aacttaaagc aataaaatgg caagagggtg
    29941 aggcaaatgg aagcaggaaa agtattacag gaagtaaaag cactatgtgc agataaaaaa
    30001 tatgaagaag cgaaaagcct gatcgaaaag aacaaagaaa atttggaaga aaagtacccg
    30061 atcgcacaag aatttataga attaaaacaa ggaacgatcc ttcaccgatt caaacatttt
    30121 tttggtataa cagaatagaa aaaacgatta aagatcggca gaaatttgct gcatctttaa
    30181 tcgttttctt tttaaacatg cgagggattc aattctgatt cttatataaa aaatagatcc
    30241 aaataataag aaaaattact gaaagcgtca attcgatgat gtgtgttatt aatacagacg
    30301 ttgtttccca atgcctaaaa gaaaaaattt gataagtaaa atatacagca ggtaaagagg
    30361 ccagtaaaac tgaaaacttc ttttttgtcc attttatgta atagaaaaga atgcttatca
    30421 taataaaaaa aggaatataa aaaacaaatt gaccgatcgc gatcatacta ttgcctccga
    30481 taaataaagt ccatgataca aaataacact ttgtgcgaaa tggcgcaagc agttttgtgg
    30541 atttatccgg taaaatctgt atcgattttg aaattagagg agaaacttgc tataatcaaa
    30601 gtgaaagaag gagtgggatt ttcatggatc atgtagtgtt agaaatcgat ttgcatttga
    30661 tcaataatat tcgggtgatt tactatgttg taagtaacag tgtcgaacaa cgtgtactga
    30721 cgaataaaat aaatggtatt ttagcaaaga aagacgttca ccgattcaat aatggtgaag
    30781 gaagttatta tagtatccca gtagaaaaaa ttatttatac gacagtcaaa gtcagagaag
    30841 atctagatac caaaaaagca tatgaaccta tttttacaac ttattgattg acagcagatg
    30901 gaggaaaaaa atgaatccat atgaacaact tattcaagat ttgatcgatg gaaaaatcga
    30961 aaagattgat gtaaaaagag aagaattaat ggcttttcga gaagcgtggc tgaagttgga
    31021 gaatcgtaaa tatgtggtgg gaaaagcctc actgaacggc aatgtgactt atcattacga
    31081 tccgacacgt ttattttagt atgaagtatt cagaaaagaa ccaaaggagg acgatgcagc
    31141 agcattgtcc tcctttgatt ttacaaaagt gaaagtttta cttatttgaa tagattttta
    31201 aatttgtttt tgacactgtc gatatttaca tcttttaaaa gattttgggc tttctcttta
    31261 taaggtccta attgttcttt atgttcttcg ataaatcgtt ttgcgccatc gaaattgccg
    31321 ttcttaatca tttccttgac tttttgaaat aattcttctt gattcatttt gaacacatcc
    31381 tttgctatca acatgatttt ttacatggac aaaacgagaa aaactgattt atccacacgt
    31441 caataatatc aaataacgat catttttact aaaaaaacac tcgattctcg cttctaaagt
    31501 aggagaaaaa tctccaactt gggacggatg gaatttcttt aactgaccca tatactgaat
    31561 atataacaag gaggtagaaa gatgaaagtg attaaatatt tgattggact ggcggcgatt
    31621 agtggtattt tgctatttgg actgaaatat tatactgaag gaagttatgg agaaatccct
    31681 ggtatacttg atcagctaaa tccattagtt gaaaaaggag aggtctatgt aaagacacaa
    31741 aaaccagagg aagtaaatga atatggtaca gcaaggtatg tgcagaaagc tgctgatgcc
    31801 aatgggaaag aacgaacggt tgaatttaat ggtctttctg tgttaaaaga aaatcattat
    31861 ttgaaaatta gcaataaagg agcacacata gaaacgtatg aggaagttcc tagagaaaaa
    31921 gttccggaga aagcattagc aattattggg taaatcagtt tttgtattaa gaatacgccc
    31981 gattttatta ttcataattt aattatgtaa actaaataca agtagtaaaa ataatacaat
    32041 aatccccctg caaaaagatt gttcttttac agggggttca ttttttcagc aagcacatca
    32101 aattgggatg gcagggttcg aacctacaat tgcttgattc agagtcaagt gccttaccag
    32161 tttggctaca tcccatttat tcaacaaaat aatcatagca ctttcctaaa aaaacgcaag
    32221 ttatttttct tgatcggata gttgttgctt tactcgtaaa aggcggcgta ctagtttctt
    32281 ttctaatcca gcaggaaggg caatcagttc tgctactgac aagacggtgt ccaataactg
    32341 atattctttg acaggttcat tcataatatg atagatgttt gctaaacgtt caatatattc
    32401 aaagcgagta tactctgttc gagcaattag aaatttcaat aaatcggtgg ctttctcgta
    32461 ctcttgatct gcgtaataaa caagagattg acgatacaat ttgatttcca gttcattgag
    32521 ggtagcaggt ctgcgacgct cgttccactc attaatagag actttacctt gaacatttcc
    32581 cttgcagtca cggacaaaaa taatcggaat ttcatttgga tccatttttt tatgtgtgaa
    32641 tttttccatt atctacgctc ccttcaaagg tttggtatct tgagtataag ggatttttgt
    32701 ttgaattact agcttttctc caaagttagc atctgtccac ccattgcagt gaaaatcgcc
    32761 gaaaaatcgt tgatcggtac tgctcgattt tttacaccat agggatcgtt gacataaata
    32821 ttttctttgt catatccagt gactacacaa gcatgaacaa gaggagtgac tcttacttct
    32881 ccattatttg tggtccaagt cataaaatca gtttcatctg ggacttcaaa attgatagtc
    32941 acagaagtcc agacaggatt tcctttagat agtgcttttt tgatatcatc gagttgagca
    33001 tcggtttttg gaaaagtgtg ataatccgtt tggacgattt gctgagcaag ggaggcaact
    33061 gggggaacat agacgaccat agcccagtct ccgccactga tatcaccaac aaatccttca
    33121 ttaggatcgc catgagtccc atctgcattg aaaacaggga cgtaatccaa ttgctcagct
    33181 aactggtttt tagttgtttc ataaccataa aaagataaaa gcatagaaag cgaagtgact
    33241 tcacagccat tttctaaaga aggagaatca aactggctct ctaatggaac atctagtaaa
    33301 acttctgctt tctcttttgt ctttttcgtt ttttgtgttg aacttttttg tgcagtgttc
    33361 attgtatgtt ctggagaatg gatatgtagg taataacttg taccgcttaa taaaagagca
    33421 gctgtgcccc ctagaaacaa tagttttt
//
LOCUS       DACDIE010000027        32533 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.027, whole genome shotgun sequence.
ACCESSION   DACDIE010000027 DACDIE010000000
VERSION     DACDIE010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.027"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            131..505
                     /locus_tag="IV250_001396"
     CDS             131..505
                     /locus_tag="IV250_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP8768688.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="IV250_001397"
     CDS             complement(619..1248)
                     /locus_tag="IV250_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP8768689.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="IV250_001398"
     tRNA            complement(1382..1454)
                     /locus_tag="IV250_001398"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
     gene            1668..1741
                     /locus_tag="IV250_001399"
     tRNA            1668..1741
                     /locus_tag="IV250_001399"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1702..1704,aa:Asn,seq:gtt)
     gene            1696..1845
                     /locus_tag="IV250_001400"
     CDS             1696..1845
                     /locus_tag="IV250_001400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8768690.1"
                     /translation="MTVNHDVVGSSPTAGVNKAYAKKKKSNHLGFFFFYYYDESSFLF
                     LSRML"
     gene            2338..3666
                     /locus_tag="IV250_001401"
     CDS             2338..3666
                     /locus_tag="IV250_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP8768691.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3868..4701
                     /locus_tag="IV250_001402"
     CDS             3868..4701
                     /locus_tag="IV250_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAP8768692.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4717..5454
                     /locus_tag="IV250_001403"
     CDS             4717..5454
                     /locus_tag="IV250_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAP8768693.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5609..6577)
                     /locus_tag="IV250_001404"
     CDS             complement(5609..6577)
                     /locus_tag="IV250_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAP8768694.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            6809..7642
                     /locus_tag="IV250_001405"
     CDS             6809..7642
                     /locus_tag="IV250_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAP8768695.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            7676..8515
                     /locus_tag="IV250_001406"
     CDS             7676..8515
                     /locus_tag="IV250_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8768696.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            8541..9101
                     /locus_tag="IV250_001407"
     CDS             8541..9101
                     /locus_tag="IV250_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP8768697.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            9300..11021
                     /locus_tag="IV250_001408"
     CDS             9300..11021
                     /locus_tag="IV250_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAP8768698.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            11186..12328
                     /locus_tag="IV250_001409"
     CDS             11186..12328
                     /locus_tag="IV250_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP8768699.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            12344..13555
                     /gene="thiI"
                     /locus_tag="IV250_001410"
     CDS             12344..13555
                     /gene="thiI"
                     /locus_tag="IV250_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAP8768700.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            14128..14775
                     /locus_tag="IV250_001411"
     CDS             14128..14775
                     /locus_tag="IV250_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP8768701.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     gene            15168..17813
                     /locus_tag="IV250_001412"
     CDS             15168..17813
                     /locus_tag="IV250_001412"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAP8768702.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FYRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            18123..19436
                     /locus_tag="IV250_001413"
     CDS             18123..19436
                     /locus_tag="IV250_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAP8768703.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            19426..20079
                     /locus_tag="IV250_001414"
     CDS             19426..20079
                     /locus_tag="IV250_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP8768704.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            20134..20826
                     /locus_tag="IV250_001415"
     CDS             20134..20826
                     /locus_tag="IV250_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HAP8768705.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            20976..21176
                     /locus_tag="IV250_001416"
     CDS             20976..21176
                     /locus_tag="IV250_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP8768706.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(21430..23109)
                     /locus_tag="IV250_001417"
     CDS             complement(21430..23109)
                     /locus_tag="IV250_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP8768707.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(23111..23323)
                     /locus_tag="IV250_001418"
     CDS             complement(23111..23323)
                     /locus_tag="IV250_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAP8768708.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            23716..25446
                     /gene="efrA"
                     /locus_tag="IV250_001419"
     CDS             23716..25446
                     /gene="efrA"
                     /locus_tag="IV250_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAP8768709.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            25443..27203
                     /gene="efrB"
                     /locus_tag="IV250_001420"
     CDS             25443..27203
                     /gene="efrB"
                     /locus_tag="IV250_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAP8768710.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            27298..27495
                     /locus_tag="IV250_001421"
     CDS             27298..27495
                     /locus_tag="IV250_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8768711.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            27648..28133
                     /locus_tag="IV250_001422"
     CDS             27648..28133
                     /locus_tag="IV250_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAP8768712.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(28237..28830)
                     /gene="clpP"
                     /locus_tag="IV250_001423"
     CDS             complement(28237..28830)
                     /gene="clpP"
                     /locus_tag="IV250_001423"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAP8768713.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            29010..29537
                     /locus_tag="IV250_001424"
     CDS             29010..29537
                     /locus_tag="IV250_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAP8768714.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            29628..30365
                     /locus_tag="IV250_001425"
     CDS             29628..30365
                     /locus_tag="IV250_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP8768715.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            30369..31286
                     /gene="miaA"
                     /locus_tag="IV250_001426"
     CDS             30369..31286
                     /gene="miaA"
                     /locus_tag="IV250_001426"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAP8768716.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            31288..32517
                     /gene="hflX"
                     /locus_tag="IV250_001427"
     CDS             31288..32517
                     /gene="hflX"
                     /locus_tag="IV250_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAP8768717.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT        10772 a         5522 c         6873 g         9366 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt acttgattcc ggcatagctc
     1681 agttggtagt agcgcatgac tgttaatcat gatgtcgtag gttcgagtcc tactgccgga
     1741 gtaaataaag cttatgcaaa gaaaaagaaa tccaatcact tgggtttctt tttcttttat
     1801 tattatgatg aatcaagctt tctgtttttg tcacgtatgt tgtaatcttt acgaaagagt
     1861 agagttggct actcataatc aaccatactt cccatcatcg ctaaatacga aataaatcag
     1921 cattcctaca ccaatcaaag cacaagctgc accaagaaaa gggatgacat tcccaaagtt
     1981 atctgtattc ataagtatgc caccacccac aaacaaaaag ccgccagcaa tcaggttttt
     2041 cattttattc atctcctttc ttacgttata gtatttcctt ctttcaatga gtattttgaa
     2101 agctatctta ttgtatgatg ttcttctagg cctcttcttt attatacagg ccattttcgt
     2161 tatctcatag aaatattcct ttttatctga aaactattgt ataccgtatg acatttttct
     2221 tcatgaagtt gtaagtatat tctcattcaa atgaaaattt catcatagaa gagattgtat
     2281 ttctaaattc ttaagagtaa aatacgaagt ataacttgga tttggaagga tgattggatg
     2341 aaagctatta cgcctgatat gacaacaaaa tttaacaaac agtttcaagc aaatatcaaa
     2401 cagcaggcat tgcagcgggc agttgtgaag aacggaatta ctgcttctgc tgaaaatgtt
     2461 tcagcacatg tggagaatac accggtattt tctatagatt tggctacggg gaaagtggct
     2521 aatcaaatgc aatcggggcg ttgctggatg tttgctgcac tgaacacatt ccgtcataag
     2581 atgctgaatc aattccattt aaaagagttt gaattatcac aaaattacac ttttttctgg
     2641 gataaatatg aaaaggcaaa ttatttctat gaaaacatca ttgcaacagg aaaagaggca
     2701 atcgatagcc ggaaagtagc tttcttactc gctactcctc aacaagatgg cggccaatgg
     2761 gatatgatcg tttcattatt ccaaaaatat ggcgttgtgc ctaaatcggt gatgccggaa
     2821 agtgcgaata gttcaaattc tcgagatttg aataactact tgaacaaatt actgcgtaaa
     2881 gatgcagtag tgttacggaa aatggttgct caaggagaaa cattagacgc tattgaagaa
     2941 aagaaagaag aaatgctaga aagcatttat aacttcttag ctatctcatt aggaacacct
     3001 ccaaaagaat tcgattttga gtaccgcgat gaagaaaaga actatcatct tgaccgagga
     3061 ctgacaccac aaatttttta tgataaatac atcggtgtaa aattagatga ttatgttagc
     3121 gtgatcaatg caccaacaaa agacaaacca ttcaatcgtt cttatacagt agagatgcta
     3181 ggaaatgtcg ttggcgggaa agaagtcaaa tatctgaatg ttgatatgga aactttcaaa
     3241 aaattagcga ttgctcagct tgaagaagga gaatctgttt ggtttggttg tgatgtaggt
     3301 caatcttcta ctagaactag tggcatcatg gccttagatg cttattcaat ggatgatcta
     3361 tttgatacgg attttacaat gactaaagct gaacgtttag attttggaga aagtctgatg
     3421 acccatgcga tggtattgac cggtgttgac ttagtagacg ggcaatcaac aaaatggaaa
     3481 gtcgaaaata gctggggcga aaaagttgga aagaatggct tctttgttat gagtgatgcc
     3541 tggatggacg aatatacgta ccaaatcgtt gtaagaaaag atttattgac gaaagagcaa
     3601 ttaaatgcgt tcaatgaaga accaattgtt ctatctccgt gggatccaat gggtgcttta
     3661 gcatagacga gtatttcaat gaagagtatg tagtaaagat aaaaagagct ggcttaagcc
     3721 agctcttttt atctttaaag tccattccct gttttttatg gtccctgtgc tataattcta
     3781 aataaaggat aaggattgga aataatcctt ttatcattaa aaatatgatg acgaagtaga
     3841 ataaaattgg gagtaggggg gaaagtcatg gatatccaaa ctgttttatc tgatctgaaa
     3901 ttaaatggta aagtgattcc agttgttggt ggcgatgtta atcagaccta tcgtataaaa
     3961 accgaacatc gtgcttattt tttaaaaatc catccgaatg taaaaaaagg attttttgaa
     4021 gcagaagtag acggattaaa agaactgtca gcattcgtac gtgtaccgga tacgtatatg
     4081 ctaggtgaaa caagtgaagg cgcttatttg ttaatggaat ggatcgagcc gggaaaaggg
     4141 gatcaacgag atcttgctgc agctttagct aatctccatc agcaaacagc gccacaattt
     4201 ggttttcgta aagataacta tttgggtaca cttgttcaga aaaattcttt tgaagaagat
     4261 tggtggactt ttttctttaa agatcggctt gaatcccaga tttctttggc agaagaaacg
     4321 aatcgatgga atgtacagcg tcaagaaaag tatctgcgat ttaaagaacg tgtattgaaa
     4381 tcggttgaac caaaaaagat cactccacgc ctcttgcacg gagacctgtg gagtggtaat
     4441 gtgttctttg accagcaagg gcatccagtt tttgtcgacc cagctgtttc ttatgggaat
     4501 cgggaacaag atattgcaat gagccagtta ttcggtggtt tccgaccgga atttttagat
     4561 gcttatcaaa ctatatttcc attagaaaag ggatggaaag accgactgcc gatttaccag
     4621 ttatattatc ttttggcaca tttgaatatg tttggagaaa gttacggttc ccaagtagat
     4681 caattactag aaaattttta agaggagggc gtgtttttgg caaaaaaata taccaaaaag
     4741 catgaagttt catattatga gtgtgacatc aaccaaacga tgacatttcc ttcgatgtta
     4801 ggagtagtta tcaaaacgtc tgaagatcaa agcgaccagt tgggaagagg cagtgaattc
     4861 gtcaatagct ttggacttac ttgggtcatc acgaattatt ccatggagat taatcgtctc
     4921 cctagagtcg gtgaagtaat ctcagtaacg actcaagcca tggagtacaa taagtttttc
     4981 tgttatcgga acttctggat ccatgatgaa gatgggaaag aattagtcaa aatagaatct
     5041 gttttcgtat tgatggattt tgtgaatcga aaaatgagca gtgttaatga agagatcatt
     5101 gctccttttg agagtgagaa gatcaagaaa atcaagcgtc aggaaaaaat tgaaaagatt
     5161 gattcgggct tcatgttgcc atatcgtgtt cgtttttatg atatcgatag taatcagcat
     5221 gttaataatg cgatgtattt taattggatc attgacgtat taggatatga ctttttgact
     5281 acgcatgttc cagaaaaagt aattattcga tttgataaag aagtagagta cgggaatgag
     5341 atcgaaagcc attttgaaca aatccctatc gaaaatggtg tcaaaacaaa acatgaaatc
     5401 cgaatgcaag aacagctgta ttgtgaagca aatattatat ggaaaaataa ataaaaaaga
     5461 ggttgtgaca gaagggggca acttcaagaa ataagaagaa atttacgaaa attgtttctc
     5521 aaatttttgt aaattttcga cttagttttc gaagaagcta tttttgtcac agcctctctt
     5581 tttaagttta gctattagat aatgatgttt agttttggat aaacgaaatc aattcttcac
     5641 ccgacaaacc atagtttaca gcaatcaata gttttaaacg tgctttttga ctattaatac
     5701 tgttacagaa aataatcccc attttttcta gttcttttcc cccgccttca taatcataaa
     5761 caggctctgc gattccattg aaacaacgag aaacaagtac tacagggatt ttttggacaa
     5821 gaagcttttc caaagcagct aaggcttgtg gagggagatt ccccgcacct aaagcttcga
     5881 ttaccaaacc gtctactttc gtatgggcga tggcttccaa taagtcccct tgcatcccag
     5941 catatgcttt aacgatcggg atagtcccat ctacagcgga aatgtccagt cgctttgttt
     6001 ctaaaagttc ctgcaagaat aaaattcgat tcttagttac taagccgatg ggacccaatg
     6061 taggtgttcg gaaagtagca acatttgtgg tatgggtctt tgtcacgtag cgtgcagaat
     6121 ggatctcatc gttcatgacc accaaaaccc ccttatctag cgcttcttca caacttgcga
     6181 cacgaatcgc actttcaaaa ttataaaggc cgtcacttcc aagttcatta cttgaacgca
     6241 ttgccccagt caaaacaatt ggtaatttgt tgccaatcgt tgtatcaaga aaaaaagcag
     6301 tttcttctaa tgtatctgta ccgtgtgtga tcaccactcc ggatatacct gatgcataag
     6361 cggacaagat acgttctttt aataccaaca tgtgagaagg catgatgtgt ggtgagggaa
     6421 gattgaagat atcttcaact aataattcta catgcttggg gattgtgatt tctgcatcta
     6481 ataatgggtt tgccacatca ggactaacac cgcccgtgag catgttttct ttcatggcga
     6541 tcgttccgcc ggtgtgtagt acaagtattt ttttcatttt acagacttcc ttacgaataa
     6601 ttttgcatag ccgttaaatc ttattgtata ctatataaaa gagttttgct atcagtgaag
     6661 aataacgaat agcctaactt atcataaaaa agctgacctt attaaaatgg aaaagactca
     6721 accttgacct cctaatacaa tggagtatct ggttgagttt cttttacaag aaagcagcca
     6781 aggaaatcaa tcacgaaggt ggccggaaat gatcagaatc ggattaacat cgtttaaaga
     6841 acatgacaaa ttaactggta aaaagaataa cactctttat gaatatgcag ggtttctacc
     6901 cttagtggaa ttagatactg cctattatgg aattccccga aagtcctcag ttgagaattg
     6961 gataagtgaa gtacctgaat catttcgctt tgtagtcaaa gtctatcaag gaatcagcgg
     7021 tcaaggagac tggaagcctt attatcagac cgaagaagag atgattgcgc attttttaga
     7081 tgttatgtcg ccaatggtag atgctggaaa gatgttttgc tttttagtcc aattcccctc
     7141 atcattcaaa tgtacgaaag aaaatgtcca gtacttgcgc aaacttagaa agtggtttga
     7201 gggttatcct gtttctatag aattgcggga ttattcttgg tacagcgatg aatttcttga
     7261 acgtacaaga aactttatgt ctagagccgc tttttcttta gccatcatcg atgagccaca
     7321 gttgctgaat acaacgattc cttttgatcc atttgtcacc aatccgaact ttgctctttt
     7381 tcgattccat ggtaggaatg cgcaaggatg gcaagcaaat gataaagagt ggcgtaaaaa
     7441 aagaacgtta tatcgttatt cagaaaaaga actgcaagaa ctagcacatg aagtcaaagt
     7501 agttgattct aagacaaaag aggtaggtgt gatttttaat aataattctg gtggtgatgc
     7561 ggctgaaaat gcattagcac tgcaaaaaat attaaatatc aagccagatg gactcaatcc
     7621 aacccaaatg gatctgtttt aagggcttat tttattcaag aaaggggcga agtctatgat
     7681 caaagctatt ttttttgata ttgacggaac attggtcacc aatcgttcaa aagcgttgga
     7741 atcaacaaaa caagcaattg aatatgctcg gaaaaacggc attttatgcg gtgtagcaac
     7801 ggggcgcagc ccagtaaaga tcaaagaaat cattgatgaa ttggaattag atatgtatgt
     7861 cgtgtataat ggtcaactgg tttttactgc agatcgcacg attatcgacc acccttttga
     7921 acaaaaagta ttggaacata ttgttgaatt tgcagatgag aaccatcgac aaatcgtttt
     7981 tggtgcgaga aatcggttag atggatcaac gacgatgcta ttaggacaat cgatttttat
     8041 caagcgacta gtcagctttt taccgcgtaa attcccggta cgtctgatga aaaaaatcct
     8101 ccaggttttt agccctcacc gacaaaagga tcgttatgaa acgttagaga tattaaaaga
     8161 accaatttat caatgtatat tgctcagtcc ggagtcagaa cagaaaaagt tagaacatcg
     8221 ctttcccgag tgtacgtttc agcgttcaaa tacgtatact gtagatatca tcccaaaagg
     8281 tggttcaaaa ttactaggca tccaagcttt cgcaaatgct gtcgaaattg aaatggaaga
     8341 aattatggca ttcggcgatc attacaatga tatcgagatg ctgaaaggtg taggaattgg
     8401 agtggccatg gggaatgctc agatcgaagt aaaacatgct gcagactttg tgactcaatc
     8461 aaatgaagaa gatgggatat acgaaggact gaaacacttc ggtctgattt attagtcaat
     8521 gaaagaagga aaaaatcagt atggaagcag agacaccttt tcaaaaagta gaaacattcc
     8581 acaaaacttt tgatccaaga agaccagaga tacccacggc ttttacagaa aatgaagctc
     8641 atacacgtgc tgcgttcaaa atagaagaac ttgttgaatt tgttgcagct acttcaccta
     8701 ataaagaaca actgcatgaa gctgttatgc tgctgcacga agcattagat aaagcagaag
     8761 aaaaaatact tggtaaaaca cagtggggaa gtgatccgct ggttgaacaa gtagatgcac
     8821 tgacagatat cctttacttt acttatggtt ctttttcatt actaggaata gtgcctgatc
     8881 gaatattcga aatcgtccat caggcaaata tggggaaatt atttccagat ggaaaacctc
     8941 attatgaccc tgtaacaaat aaagtgttga aacctgaaaa ctggcagatg gaatatgctc
     9001 cagaacctaa aatcgcggca gaaatccaac gtcagattca agaagcgatt gctaagaaga
     9061 aatccgctga tataggcatg tgcacaaatg aaaaagagtg atcagagttt cactattttt
     9121 gttatcttta tacgatggaa aaacaaaaga ttttcgttta agttagtgaa tgcatgaaaa
     9181 tagtatgcta taatctagaa tagatgtctt ttgcacgaga gacgtattat ttggatttat
     9241 gccaagcaga acgccatctg ttgcataaaa gatgcatact gaaaacggag gaaccataaa
     9301 tgggaaataa tgttatcatc ggaatcgtca ttgcaatcat tatagtcgcc attgtcgtct
     9361 ttttggttag ttacttgatg aagaaaaaaa accaagatcg attggatgcc ttagaaaaac
     9421 gcaaggaaga attatttgac ttgccagtga ttgaagaagt cgatgatgtt aaaaaaatgc
     9481 atctggttgg gcaaagccaa aatacctttc gtgaatggag tcaaaaatgg aatgacatat
     9541 ctacttcctc gttcgctgaa ctagaaagtc aaattttcga agtggaaaac ctcaatgaaa
     9601 gttttcggtt cttaaaagga aaaaaagcag ttgttgaagc agaagctaca atggaccaaa
     9661 tggagaaaca agtgacggaa atccgtgaag gtctaaagga attgcgggaa acggaagaac
     9721 gcaactcact agctgttcag caggcacttg acgtctatga agaactgaaa gaaaaagtca
     9781 gagaagacga gcatgaatac ggaccagctc tttttgaatt gcaaaaacag atcaaagtga
     9841 tcgaaaaaga atttacgcag tttgtgactt taaacacttc tggagaccct gttgaagccc
     9901 gcgaggtttt ggataaagca gaaaaacata cgtatgaagt ggaagctctg atgaaaaaaa
     9961 ttccaccgct gtatgaggag ctccatacca ctttccctga acaacttgaa gaaatcagtg
    10021 atacttacga gaaattgatg gaagaacaat atgtattccc agaagaagat cttgctgaag
    10081 atatcgcaaa agtaagtcga agaattgaaa attcattagc caatcttgaa aaaacagaag
    10141 ttgaaacggt cgaatttgaa aaccgtgaaa cagctgattt gattgattcg ttatatgata
    10201 tcttagaacg ggaaatggaa gctcagagat atgtaaaaac taaccaatca acgattgcag
    10261 agtatatcaa acatacaaca aagaataacc gtcaattatt gattgagctg gatcacacat
    10321 ctcaaagcta tacattgaac cacaatgaaa ttggccgtgt acgaggtttc caaaccgaag
    10381 tggaagaaat ggaacgtcaa aatgaacaga tgattccaca gatccgtcaa cacgaaattc
    10441 cttattcaga agtgcgcacg ttctataaaa cagtcttcaa agtcttagaa gatatcgaaa
    10501 agcaacaagt agagatcgac gactcattac atgaattgag aaaaggcgaa aaagaagcgc
    10561 aagaaaagat cgatacattc gaattcaaat tgcgcagttt gaaacgtttt gtagaaaaac
    10621 aacgtttgcc tggcttgccg aatgattacc tagaattctt ttttgttgct actgaccgta
    10681 ttgaagaact aagtgttgtc ttgaataaaa ttcgagtaaa catggaagag gtcaatcgtt
    10741 tagttgctct atgtgaagaa gatctagaat tattagataa gaagacacat gatttagtag
    10801 atgcagcagc attaacggag caaatgctcc aatatgcaaa tcgttatcgt catacacacg
    10861 aagaagtgag agaagcaatt gataaaagct actatttatt caataaagaa tatcactatc
    10921 aagaagcatt agacgaaatc ggtacagcat tagagcgagt agaacctggt gctttcaaac
    10981 gcatcgaaga tttttatttt aatcatccag acttagtata aagcaaaaaa gttagtaaac
    11041 cagcaagagc aagacggatt attgtcttgt tcttgtcttt ttttgtatat ttgaaaattg
    11101 aaacaagtga aaatttcacc tgctcttgaa agcaccttgg acaaccgtta taatagaaaa
    11161 gaatttcaaa aaagaggggt ttcttatgat atattttgat aacagtgcaa caaccccaat
    11221 ctttccgcaa agcttagatg catatgtaaa aaccagtcag cggattattg ggaatccatc
    11281 aagcttgcat gatttaggca atcaagctaa ccggttgttg cagcaagcaa gaaagcagat
    11341 cgctgatttg atccatgtag aaccagaaga aatctatttt acttcaggcg gaacagaagg
    11401 agataactgg gtattaaaag gaacgatgat cgaaaaaaga gaatatggga atcatatgat
    11461 catttcagga gtcgaacatc cggccgtcag cgagacagca gaacaattaa aagaattagg
    11521 aatcgaagta agtatcgccc cagtggataa acgcggattt gtacgtgtcg acgaattgaa
    11581 agaattaata agaaaagaga cagtattagt ctctgttatg gctgtgaata atgaagtagg
    11641 atcgattcag cccatccagg aaatcagtga tcttttggat gattttccta aaattcattt
    11701 ccatgtagat gctgttcagg cgattggaaa agtaccattc tcgcaatggt tgacagaaag
    11761 agttgatttt gcaacatttt cagctcataa attccatggc ccacggggaa ctggtttcat
    11821 ttattggaaa aagggtcgtc gtttagcacc attactgaca ggtggagggc aagaaagaaa
    11881 tcagcgaagt gggacagaaa atgttccagg tattgtggct atggttaaag ctttacgttt
    11941 atcattagag ataaaggaac aaaaacctga gcagacacaa cgcttaaaag tagctctaat
    12001 caatgcgttg aagacgtatg agaaagtgac gattttttca gaaggaactg actatgcacc
    12061 gcatatttta tgttttgctc taaaaggaat tcgcggcgaa gttctcgtcc atgccttaga
    12121 agaaaaacaa atatttgttt ctactactag tgcctgctcc tcacgtaaac atatggcaag
    12181 cagtacattg cacgcaatgc atgtaccaaa tgatctggct acatctgctg ttcgaatcag
    12241 cttagatgaa aacaattcgc ttgcagaagt cgagcaattt atgattattt tcaagcagct
    12301 ttataagaaa ttttctgcaa tcaattaaaa gaggagtgtt cttgtgaaat acagtgaaat
    12361 tatggtccgt tatggggaac tttcgacaaa aggaaagaat cgaaaaagct ttatcatgca
    12421 actagcacaa aatgtcagac aagctttgaa agactttcca gaaatcaaaa tccatgctga
    12481 tcgggaccgg atgcatcttt tattgaatgg cgcagacggg cagctagtta ttccaaaact
    12541 agcaaaaata tttgggatcc aaaatttctc tccaagtatt cgtgtagaaa aagatgtcga
    12601 agttttgaaa caagctgttc aagacatcat gaaagaaatt tatactggga aagaaacatt
    12661 taaaattatc gctaaaagaa gcgatcacca atttgaattg acaagtaatg aattgaatca
    12721 aacattaggt aatgctgttt ttgatatttt cccgcatatc caagttcaaa tgcgtcaacc
    12781 agatattcca ttacgtgtag agattcggcg tgatggcgct tatctatcgt atgaaacgat
    12841 caagggagct ggaggattgc ccgtaggaac aagcggaaaa ggaatgctta tgctttctgg
    12901 ggggattgat tctcctgtag cagggtactt tgccatgaag cggggagtgg aaattgaagc
    12961 tgttcatttt gctagtccgc cgtatacaag tgaacaagca ctgcaaaaag caaaagatct
    13021 aacagccaaa ctagcacctt atgtgggaac gatccatttt atcgaagtgc catttaccga
    13081 gattcaagaa gaaatcaaaa agtcagtgcc acaaggatat tggatgacaa ttactcgtcg
    13141 aatgatgttg cgtttgaccg atgaaattcg ccgtattcgt catggtttag tcattttgaa
    13201 tggtgaatca ttaggtcaag tggcttcaca aactttgcaa agtatggtgg ctatcaatga
    13261 agtaacgaat acgccaatca ttcggcctgt agcgacaatg gataaacttg aaatcattga
    13321 agtagcagaa cagatcgata catttaatct ttctatccag ccttttgaag attgctgtac
    13381 gatttttgct cctccacagc caaaaacacg ccctcaactg gaaaaggtcc atcaatatga
    13441 agagcgttta gcaatagatg aaatgatcca acgagcacta gctggcttga agatggaaga
    13501 gatacaagca gaatcgtcta atgaaaaaac tgaagaattt tcagaattgc tataagaggt
    13561 tatgaaaata gtgttttgac cagagtatta gagaaacaaa acgaaaaaat agctccatgt
    13621 attttaccgt ttttgaacta atgtcgaaga ttagctgatt gaataccatt tatcaagaag
    13681 ttatggaaaa gccggttctt ttttactaac tgaaaaataa aattttattt agagatcaag
    13741 ctacagttga taaatgatat aaaaaagaga atttcccatg aaatagcgag gttgtgacag
    13801 aagtgggcag cttcaagaaa taagaaggaa tttacgaaaa ttgcttttca aatttttgtg
    13861 aaatttcggc ttatttttcg aagaagttac ttctggaaca ccgtgtatca gaggttgcga
    13921 aaaagctgcc ctgcatcaag taataagaac agccaaacaa aaatagttcc tcatatttta
    13981 cgtttggttg agcttattac cgcagatgca agcttttgaa caccgtttta tttgtctcac
    14041 acccttttcc ttcctacttg taaccttgct cccgtgttag aaacatgtta cactaaatat
    14101 gtgaaattat taacaaggag cgtcgttatg cgagaaaaaa cagatacact gccaaaagca
    14161 actgcgaaaa gattaccttt atatttacgc tacttgaaga tgctggatga ctcaggtata
    14221 agtagaatca aatcaaatga gttcagcgaa atcacccaaa ttccttctgc aacgatccgc
    14281 agagacttct cgcaattagg cgagctaggc agaagcggat atggctatga tgtccctttt
    14341 cttatcgatg tattcaataa tatccttaat actaaagaag aaaaacgaat cgcattagta
    14401 ggatatggaa atcttggcaa agcattgaaa cacaacaatt ttagaagaaa tgaaaatcta
    14461 aatatcgtgt gcgtctttga taatgatcca gcactgatca atcgagtaat tgatggagaa
    14521 atgatctatc cgattgatcg tttcgcggag atagccaaag caaaaaatgt gacagtagca
    14581 atctctacgg taccaagtaa atattcacaa tctgctattg atgaaattgt caaaggaaat
    14641 gtaaccgcta tacttaattt tgcgcctgac agagtaactg tcccagctta tgtcaatgtt
    14701 caatacattg atttgacaac cgagttgcag acgcttattt actttgatga gaattactca
    14761 gaagtttttt cttaggaaaa tttgagtagg cttattgatc ttgcgagaac tcgtcgtaat
    14821 tctttcaaaa attttttagt actaaatgaa gtggtttttt cacgaccact tgttgactaa
    14881 aaaaaaaaac cattgtagaa tgaagcataa cgctgaacaa gaggagtaac tggcacattc
    14941 attcttagag agagaactat ctgctgaaag gttctcaatg acaaccagtg aaggtagctt
    15001 gggagtttct gtcttgaatg gagtagagat agacgagtcg tgatcgttaa ctcacttgag
    15061 aggcaataat gattgattat tgcaatttag gtggtaccgc gtaaatcata cgtcctagaa
    15121 tacgaaagta ttttaggacg ttttttagtt taaaggagga aaacagcatg acggaagaaa
    15181 ataacctatc aacaaaatat aatccgcaag aagttgaagc tggacgttat cagcattggt
    15241 tagaacaaga tttgtttaaa ccgagcggag ataaaaaagc aaaaccttat tcaatcgtaa
    15301 tcccgccccc aaatgttact ggaaaacttc atttagggca tgcttgggat acgaccttgc
    15361 aagatatgct gattcgtcaa aagagaatgc aaggatatga tactttatgg ctgcctggta
    15421 tggaccatgc aggaatcgcg acacaagcaa aagtagaagc gaagcttaga gagcaaggga
    15481 tttctcgtta tgatctaggt cgtgaaaagt tcatcgatca agtgtgggaa tggaaagaag
    15541 agtatgcgag ccacatccgc gaacagtggg ctaaaatggg cttatcttta gattacagta
    15601 gggaacgttt tacattagat gatggattat ctgaagcagt acgtaaagtt tttgttacct
    15661 tatacgaaaa aggtttgatt tatcgtggtg aatatatcat caactgggat cctcaagcac
    15721 gaacagctct atctgatatc gaagttattc ataaagatat cgaaggcgcc ttttatcata
    15781 tgagctatga actagcagat ggatcaggcg tagtagaaat tgcgacaacc cgtccagaaa
    15841 cgatgttggg agatacggcg attgctgttc atccagaaga cgagcgttat caagcgctca
    15901 tcggcaaaaa agtgattcta cctttagtca ataaacaaat cccaatcatt gcagatgaat
    15961 atgtagatat ggaattcggg acaggtgtag taaaaatcac tccagcacat gatccaaacg
    16021 actttgaggt aggtaatcgt catgatcttc cacgcgtaaa tgttatggat gatgatggta
    16081 cgatgaatga actagcagga aaatatgctg gaatggaacg ttttgctgct cgtaaagcaa
    16141 tcgtgcaaga tctgaaagaa atcggtcgat tgattaaaat cgatacgatg atccacagtg
    16201 tgggtcattc cgagcggaca ggtgtcgttg ttgaacctcg gctgtctact caatggtttg
    16261 taaaaatggc gccgttagcg aaaaaagcca ttgataacca agaaacagat caagcagtgg
    16321 aattcttccc tccaagattc aatcaaacgt ttttacgttg gatggaaaat gtccatgatt
    16381 gggtaatctc acgacaatta tggtggggac atcaaattcc tgcatggtat cacaaagaaa
    16441 caggtgaaat gtatgtaggg atggaagcac cagcggatag tgaaaactgg gtacaagatc
    16501 ctgatgtttt agacacttgg tttagttcag ctttgtggcc tttctcaaca atgggctggc
    16561 cagatacgga aagcgaagat tataagcgat acttccctac tagcacttta gtaaccggtt
    16621 atgacatcat tttcttctgg gtaagcagaa tgattttcca aagcttggaa tttactggcg
    16681 aacgtccatt tgagaatgtc ttgattcatg gattgattcg tgacgaacaa gggcggaaga
    16741 tgagtaaatc attaggtaat ggaatcgatc cgatggaagt aatcgaaaag tatggagcag
    16801 atgctctccg ctggttctta tcgaatggtt ctgcaccagg acaagatgtc cgcttcagct
    16861 atgaaaaaat ggatgcttct tggaacttta tcaacaaaat ctggaatgct tcacgtttcg
    16921 ttatcatgaa tatcgaagga atgaccgtag aagagatcga tttatctgga gaaaaatcag
    16981 tggctgaccg gtggatcctt actcgtttga atgaaacgat cgaacgagta actgagctat
    17041 tttatcgttt tgaatttggt gaagcaggtc gacaactgta taactttatc tgggatgatt
    17101 tttgtgactg gtacatcgaa atgagtaaag aagtacttta tggagatgat gctgtttcaa
    17161 aacaaaccac ccgtagcatc ttagtccata cacttgatca gatcctacgt ttattgcatc
    17221 caatcatgcc attcgttaca gaagaaatat gggaacatat tcctcatcag ggaaattctc
    17281 tagtagtggc agaatatcct gttgtacgtc cagagtttga tgatgaaaca gcttctaaag
    17341 ggatggaagt attgaaagaa ttgattcgag ccgttcgaaa tattcgttca gaagtgaata
    17401 caccattgtc taaaccaatt acgttgctaa ttaaaacaaa cgatgctaaa atcgatcaat
    17461 ttttagttga aaatacaagc tatatcgaac gcttctgtaa tcccgaagag ttgacgatat
    17521 caagtgagat cactgcgcca gatctagcga tgtcagctgt tctgacaggt gcagaaatct
    17581 tcttgccgtt agcaggattg atcaatattg aagaagaaat caaacgatta gaaaaagaac
    17641 tggaaaagtg gacaagcgaa gtaaaacgtg tccaaggaaa actttctaat gaacgttttg
    17701 tttctaacgc gccggaagaa gtagtagagg cagagcgtgc aaaagaaaaa gattatttag
    17761 agaagcaaac ggctgtcaaa gaacgaatcg aaagtttgcg tactattcaa taagaataga
    17821 aaaagagagt gtgaaatcag ctagctatta ctaaaagcta gctgattgaa catcgttaca
    17881 ggaaagagtt tgagcctcac tcttagcttc gattcataag gagatgtgac ataaattttg
    17941 tcgcatctct tttttagtct actcttatta tgtaggagaa ggagggaggt ggatcatgta
    18001 tcaagagtca ataaagtagt tgataaaatc gttcctctct ctgtttcttt tctctttgtc
    18061 atacttttat ttttcttatg tgttctattt gttataataa aagagaaatg ggggaattgt
    18121 tgatgacgat tgaagaagca atagaatgga tccatagtcg attaccattt ggttcacgtc
    18181 ctggccttga ccgagtcgaa gctttattag aagtggtagg gcatccagag agaaaagtaa
    18241 aaacgatcca tatcgctgga acaaacggaa aaggttctac tgtagcttat ttgagaagcc
    18301 tattagaaga ggctgggctg acagtaggta catttacttc accttatatc gaaacgttca
    18361 atgaacggat cgcaatcaat ggcaaaagca ttccagacaa tgatttgatc cagcttgtaa
    18421 aaaaatatca gccactgatc aaacaacttg atcaatttga agcagtagct ggtattactg
    18481 aattcgagac attaacagca atggcatttg attatttttt gatgaagaaa gtggacgtag
    18541 cgattatcga agttggctta ggcggcttat tggattctac caatgtagct gtaccaatgc
    18601 tgacgggaat aacaacgatt ggacttgatc ataccgaaat acttggagat acaattgaag
    18661 aaatcgctgc acaaaaagct ggaatcatca aacaaggcgt tccagtagta acaggaaata
    18721 ttgccccaga agcttttact gtcatccaac aaacagctaa agcaaaaaat gcaccgcttt
    18781 atgtatacgg ggaaaattat aaaataaact atctgcatcc tgatgagatg tggggagaag
    18841 tatttgaatt ttttaatgaa ataggaaaga tccctcaatt aaccactccg cttttagggc
    18901 aacatcaagc tgaaaatgca ggagtagcca tcgaattgta ttttgtttac tgtcaattgg
    18961 aaaagctgcc tttccgagaa aaagatgttt tgaagggatt gaagaaatct cgatggccag
    19021 gaagaatgga gcgtttgagt aacgagccat tagttgtttt agatggtgcc cataatacgc
    19081 atgcaatgaa ccggcttgtt gagaatttga aaaaagaatt caaaggatat caaatcaaca
    19141 ttttgttttc cgccttacga actaaaaatg ttagagaaat gctagaacga ttgcagcaag
    19201 taccaaatgt ccatatttat ttgacgacct ttgaacatcc aaaagccatc gaattaagtg
    19261 aagtatcaga tttagcaagt gagagagtat caatcgtttc attgtggcaa ttcggattag
    19321 cagatatatt ggaaaagatg acaaatgaag acgtattagt tgtgaccggt tcactttatt
    19381 tcatttcaga agttcgccat ttattaatac aattaggagg agatcatgaa gtataatgca
    19441 gtgatttttg acatggatgg tttgctgttc gacacagagt tagtgtatta cgaagccaca
    19501 caagtagtag cagacaagat gggttttcct tatgataaag acctgtattt aaaattctta
    19561 ggtgtttcag atgaagaagt atgggcgaat tatcatatga ttttctcgga atttggcaaa
    19621 acaagtgtac aaagattcat agatgattca taccaggaaa cgcttgaacg tttttcgcaa
    19681 ggagcggtac aaatgaagcc cggtgtgttg gaactcctct cttttttaga agaacatcag
    19741 attcccaaag ttgtcgcttc cagtaaccaa cgagcagtca ttgaactttt attgaagaag
    19801 aacaatctgt ttgaacgatt tgagacgatt gtttctgcag aaaatgttca acgagcaaaa
    19861 ccagacccag aaattttctt gttagctaat cagaagctag gtacagcgaa gaaagaaaca
    19921 ttgattttag aagattcaca aaatggtatt ttagcagctt ctgcggctga gatcccggta
    19981 gtcatggtac cagatctgct tccaccatcc caagaattga tcaaaaaaac agctggggtt
    20041 gtttcttctt tacatgaaat ccctgcattg ttaaaataaa aatccatctc ttttcgtatc
    20101 ttattttaga taagatagaa aggagatgtt tttttgggaa aacgattgat caaagaagta
    20161 cctactagct ctttaccaag ggaacgtatg gaaatttatg gcgcggaagc tttatcggat
    20221 caggagcttt tggcaatctt actgcgtacc ggtcaacacc cttatagtgt gatgtcgatt
    20281 gctggaaatc tgttaaaaac gtttggagga ctggcctctt tacgacaggc tactttgcat
    20341 gagttagaag agattcgagg gataggacgc gtgaaagcaa tcgaaatcaa agccttgatc
    20401 gaattaggtc gacgaattca gacggatcat gaaagagcgg ctccaccggt tcgtaccagc
    20461 tacgaattgg cccagcaatt gattctggaa atgaaagatc acaaacaaga acatttggtt
    20521 tgtatttatt tggacacgaa gaatcaagtg atcttaaaaa aaacagtgtt catcggttcg
    20581 ctgaatcagt cagtcgctca tcctagagaa attttccatt atgctgtacg ttattgtgca
    20641 gcaaggatcg ttttagcgca taatcatcct tctggtaatg ttattccatc gcagcaggat
    20701 atgaatttta ccaaaaggat acaaaagtgt ggagaaatga tggggatcac tgtactggac
    20761 cacttgatca tcggacgcaa acgatatttt agtttgagag aagaaggaat gatggaagag
    20821 aagtaagttg ctttcttgca aaaaaaatga agcaagtgta caattatatt gtaattttgt
    20881 ttgggaatag aagttgaaac atctgttgca cctctatcgt acacgagcaa tgttacaatt
    20941 taaattagtg cttttggcac gaggaggata ttattatgga aaacggaaca gtaaaatggt
    21001 tcaacgctga aaaaggattt ggatttatct cacgtgaaga tggaagcgac gtattcgttc
    21061 acttctcagc tatccaaggt gacggattca aaacattaga agaaggtcaa gcagtgactt
    21121 tcgacgttga agaatcagac cgcggcccac aagctgcaaa cgttgtaaaa gcttaatttt
    21181 atctgtaaat caaattgatg gtagacatca atttttacga gagaacaaag cgagtaacaa
    21241 aaatacctgc cacaagagac aatttctctt gtggcaggta tttttttgta tgaaaaagta
    21301 aataaattta agaggctatg aaagagccac cttgtatcaa ggaataagaa caaacaaaaa
    21361 tggcttctca tgtttttcat ttgttttctc ttatattcag gacaaggtgc tgatttcata
    21421 acctcttatt tattcttcat ctggtgtcat taccattggc aaaatcattg gatgtctttc
    21481 tgtacgttcg aataagaaag gctgaagcgc gccagtcatc gcttctttca atttatattc
    21541 tgaacagtca ggtccattca aacattctct taaagcgtgg aataagattc gctgagcttc
    21601 attgatcatt tctcctgatt ctcgcatgta gacaaatcca cgagagagaa tatctggacc
    21661 agccaagatt tcttttttct tgatatccac tgtcgcgaca gctaatacaa gaccttcttc
    21721 tgaaagcagg tgacggtcac gtaaaacgac attaccaata tcaccgatcc cgctaccatc
    21781 gatatacaca tctccagcca caaaatgccc agcaggacgc gcagaatcgc tagttaacgc
    21841 taaaacatcg ccattttcca tgatgaagca gttttctgca ggaacaccta catcttgtgc
    21901 tagagaagca tgtactttca acatgcggaa ttctccgtgg acaggaataa aatattttgg
    21961 cttcatcaaa cgaagcatta atttttgttc ttcttgtcca ccgtgaccgg aagtatggat
    22021 gttattgatt tttccgtgaa tcacttctgc accagcttca gacagcaaat tgatcaaatg
    22081 attgacactt gttgtattac caggaatagg tgaacttgaa aagatcactg tatcgtctgg
    22141 ctggatttgg atttgacggt gagtaccatt cgcgatgcgg ctcaacgcgg ccatcgcttc
    22201 tccttgagaa cctgtacata ggatcatcgt ttcattggca ggcaagcgat tgatttcgtg
    22261 tgcatctaca aatgtgccgt ctggaacttt gatatatcct aaacgttgac cgttgacgat
    22321 ggcattttcc atgcttcgtc cgaaaacagc gattttacga ccagttgcca cagcggcatc
    22381 tgctgcttgc tgcaaacgga agatgttaga agcaaagctg gcaaaaatga tacgtccttc
    22441 gatttttgaa aagatcttca aaatagaatg cccaatcgtt ttttctgatt tagtaaatgt
    22501 tggaatctca gcatttgtac tatcagataa aaggcacaag actccttctt ccccgatacg
    22561 agccatttta tgcaagtttg ctggttcgcc aacaggagtg aaatcgaatt tgaagtctcc
    22621 agtcgctacg atatttccag aaggtgtttt caccacgatc cctaatgcat ctgggatact
    22681 atgggtcgtt ctgaaaaagc tgacagaagt ctttctaaaa cggatgatcg tatcttcatt
    22741 gatctcatgg agctctgcat ctcgaagcaa gccatgctca tctaatttgt ttgtgatcaa
    22801 cgctaatgcg agaggaccag catagatagg aatatttgct tgtttcagca agtagggtat
    22861 tccgccgatg tggtcttcgt gcccgtgagt aatcaccaag gcttttactt tgtgtaagtt
    22921 ttgtatgatg tagctgtagt cgggaatgac atagtcaatt ccaagtaaat cgtcttccgg
    22981 gaatttgatc ccggcatcaa tcaaaatgat ttcatcttgg aattgtaccc catacgtatt
    23041 tttcccgatt tcacctaatc caccaatagc aaagacgcct gtctcgttat ttttgatgga
    23101 aagtttcata ttaaaactcc actaatgaga aatcagcatt ttctttttcg tactctagat
    23161 gacttccttc tagaagttga acgaattcaa tattgtatgg ggtattttct tccacacgtt
    23221 ggcgagcgtc tacatcgctt tttgcctcaa tgtaaagaga ttttgtttct tcccgtttag
    23281 ggttgcgtac ttttgtttct tgataataaa ctttatagat cattgttttg tctcctttat
    23341 ctgcgtaaag cagtgattta gctagttgat ttttttagac aacacaaaac gaggggacaa
    23401 gccacctggt ttttcatatg ctttgactag aatactggat tcgagtcaaa gagacaagcc
    23461 tcgtttttgc ttatctgttc ggatattttt tcaaccgtac cttctgtata aaaaatatac
    23521 aacaattaat tgttattcta gcataaaaca gcaaataagt atagaaatca actagattga
    23581 aagaaaaaat caatgtttat catcccgcta taatcataat gactttttta tgagagaagt
    23641 gttctattaa taggctcgtt gtgctatcat ttttagtaat gaaaatgtaa caaaagatta
    23701 aaaggagatg agaaagtgaa actaatgtgg cattatacca tgcgttataa gaaattcttg
    23761 tttttcaatt tcatatgcgt atttggtttt attttgattg aattaggact tcctacgatt
    23821 ttagcacgta tgatcgatgt aggaatccgc aatgatgatt ttgattatgt gaagcaacaa
    23881 gggatattta tgatcgttat tactgtaatc ggtattattt tgaatatctt gttaggttat
    23941 tttggttcga ggattacgac taatatcgta gcagatattc gagatgattt gttcgaaaag
    24001 atccaaagtt attcgcatca ggaatacgaa aatctaggcg tatcttcatt gatcactcgt
    24061 acaacaaacg atgcttatca gatcatgttg ttcctgcaaa atattttacg tatcggtttt
    24121 atggcaccaa tgatgttcat tgtcagtttg tacatggtca tgcgtacaag tgtgacactc
    24181 agtttgtatg tagtaggtgc attaccttta ctattattgg cagtcgtagc gattgctaag
    24241 gtgtctgaac cgttatctaa aaagcaacaa aaaaacttag ataaaatcaa cagtatcttg
    24301 cgggaaaatc tttcaggatt gcgtgtgatt cgtgcattcg tcaatgaaaa gtttgaagaa
    24361 aaacgttttg ctgtagtcaa tgagaattat gcttctagtt caaaaagctt attccgtttg
    24421 atggctgttg cacaaccggg tttttatttc ttatttaata tcgtaatggt attgatcatc
    24481 tggagcgggg cagtgcaaat cgatgcaggc gagcttcgtg taggagattt gattgcgttt
    24541 atcgagtata tattccatgc cttgttctca ttcatgttat ttgctagtgt gttcatgatg
    24601 tatccaagag cagctgtttc agctggacgt atccaagaag ccctagatgc caaaccttcg
    24661 attgtagaag atccaaatgg tatatctgat acaaaaacta aagggtatat cgaatttcaa
    24721 aatgtaacat ttgcttatcc aggccattcg caagagccag tgatacgaaa tgtcagtttt
    24781 acagcctctc caggagagac cgttgctttt attggaagta ccggtagcgg aaaatctacg
    24841 ttgatccaat tgatccctcg cttttatgat gtgagtgaag gaaagatctt gatcgatgga
    24901 gtagacgtga gagattatcg gctaagcaag ttgcgagata agattgggtt tattccgcaa
    24961 aaagcgctgc tatttactgg aacgattgca gataatttgc gatacgggaa agaagatgct
    25021 acgcaagaag aaatggaacg tgcagctgag attgctcaag cttcagaatt catttctcga
    25081 aaaccagatg gatttaatga acatttatca gaaggtggtt ccaatttttc aggtggacaa
    25141 aaacaacgat tggcaatagc cagagccatc atacgccgtc cggaaatcta tatttttgat
    25201 gacagctttt ctgcattaga ttatcaaacg gatgcgaagt tacgtgctcg tctgaaaaaa
    25261 gaaacgacag aatctactgt attgatcgta gcacaacgtg tgggaacgat catgcatgca
    25321 gataagatca tcgtattaaa tgaaggagaa gtcgtcggta tggggacgca tcgggagttg
    25381 ttggaaaaat gcccgattta ttatgatatt gccgcttctc aattatcaaa ggaggaatta
    25441 gcatgaaaaa aaccttttct tccttaaaac gtttagctcg atatatcaga ccgtatcgtg
    25501 agacttttct tttagtgatc ctatttacag ttctgacagt ggctttcaac gtggctttgc
    25561 catatgttac aggacttcct actacagaaa tcagccgtaa tattgccaat ggcgaatcaa
    25621 tcaatttttc ttatatattc aaatgtctca tttggatcac agttgttgga atagggtatt
    25681 gtatttcgca attactttct ggtgttttga tgacaaatgt cgttcaaagt gccatgcatg
    25741 atttgagaag agacatagaa gaaaaaatca atcgtcttcc ggtttcatat ttcgataaaa
    25801 accagcaagg aaatattttg tctcgtgtga cgaatgatgt agatgcagtc agcggtgctt
    25861 tacaacaagc ttttattggt atcgtaaatg cgatattggg aattacgttg gctgcagcaa
    25921 tgatgtttta tatccagccg atgatggcgc tgatctctat gatcatgatt ccaacatcga
    25981 tctggatctc aaaacgagtg atcaatgctt cgcagaaata ttttcaaagc atgcagaatt
    26041 ctcttggtga gctaaacggc tacgtccaag aaaatatgac aggttttagt gtattgaaag
    26101 tatatggacg cgaaaaagaa acattggaag gattcaaaaa agttaatcac agcttgaaat
    26161 actttgggtt tcgtgcagca ttcatttctg gcttgatgat gcctctagtt caattaactg
    26221 cctatgctac ttatattggt atggctgttc taggaagctt ttatgttatt actggggcga
    26281 tcgttgtcgg tcaactacaa gcattcatcc aatatatttg gcaaatcagt cagccaatgg
    26341 ggaacgtgac acaattgtct tcagttttgc aaagtgcatc agcggcaacg aaacgtgtat
    26401 tcgagatttt agatgagcca gaagaaaaag aaaacaaagt agatgcttca ctgccagaaa
    26461 cgattttagg agatgtgaca ttcgatcatg tcgattttgc atatgatccg aagaaaccat
    26521 taatccaaga tttgaatttt gaagtgaaag ctggtcaaac cgtagctgtg gtcggtccga
    26581 ccggtgccgg caagacgaca ttgatcaatc tattgatgcg tttttatgac gtagacaaag
    26641 gagcaataag gattgatgga atcgacacca aatcaatgag tcgcagtgat gtgcgttcgc
    26701 tatttggtat ggtcttgcaa gatgcatggc tttatcaagg aacgattgca gataatatcc
    26761 gcttcggaaa gcttgatgcg acagattatg aagtagttga tgctgcgaaa acagcaaacg
    26821 tagatcattt tatccgaacg atgccagatg gatatgatat ggccatcaat tctgagggag
    26881 acaatgtgtc tttaggacaa aaacagttgc tgacgattgc ccgagcagtg gtctccgatc
    26941 ctaaaatctt gattttagat gaagcaacaa gttcagtaga cacccgttta gaagcgttga
    27001 ttcaaaaagc aatggatcgc gtgatggagg gacggacaag tttcgtcatc gctcaccgtt
    27061 tatcgacaat ccgtgatgca gatctgattc tagtgatgga tcaaggtcag atcattgaaa
    27121 aaggaacgca tgagagttta ttggcacaag gtggatttta tgaaaaactg tataacagcc
    27181 aatttgctga ggaagcagaa taagtcatat aagaataaaa cagtttaaaa cttatttttt
    27241 gttctctatt cttgataatg gtaaagtaaa gttatcaaga aaagtgagga cacggagatg
    27301 gaacagtttt taaatgaaaa aattactgaa gccaaaagag aagttgatcg tttaacgcac
    27361 gtctgtcaga cgaagccttt tgattcagct gatttttttg aaaatgagtt tcaaatcgaa
    27421 cgcttggcag cacaaataga agtgtacgaa ttaattttaa aaaaagtaat agaagaaaat
    27481 atagatgtac aataaaaaca ggtaaaacgt cagttaccag atactgtaaa tagacatctg
    27541 gcctgagcag gttttacctg ttttttttct tatgaaataa atatatgcta tgataccttt
    27601 attcaatagg attagtttct acaaaaaatg aaaggagaaa aataaatatg gaacggttta
    27661 tagcatcaaa tgccacagtg attggtgatg tgacattgtc agaagatgtg acgatctggt
    27721 atcaagctgt tttacgcggg gacagtaatt ggataaaaat aggacaaaga acgaatatcc
    27781 aagatggtac aatcatccat gtcgatcatg atgcacctgt agatattgcg gagaatgtga
    27841 cggtaggaca tcagtgcatg ctacatggct gtacaatcga aaaaggggcg ctgatcggga
    27901 tggggaccac gatattgaat catgcggtta ttggtgagaa tagtttgatt ggtgctggat
    27961 cattagtaac agaaggaaaa gttattcctc ctaatgtctt ggcttttggt cgcccagcta
    28021 aagtgatccg tcctttgaca aaagaagaaa tacaaaaaaa caaagaaaac atccagcatt
    28081 atgtcgagat aggtcggatg catgcgcaag gaaaattcaa ggagcaaaga taggcagtag
    28141 catccataaa ataaaaaagg atggtcagtt tgagttgacc atcctttttt atttggttta
    28201 gataagagag cttaaaaaag cttcctttga aacagattaa tttaatgaag aactgttttc
    28261 catgacttca tcgatcaatc catatgcttt tgcttgttca gctgtcatat agttgtcacg
    28321 atctgtatct ttttcaatca cttcaagcgg ttgtccagtg cgttcagcta aaattttgtt
    28381 taagcgttcg cgtgtttgca aaatatggcg tgccgcaatt tcgatttcag ttgcttgccc
    28441 ttgagcgccg ccaagaggtt gatggatcat gatctcagca tttggtaaag caaatcgttt
    28501 accttttgta ccagctgtca gcaagaagct tcccattgaa gcagccatac ccataacaat
    28561 tgtttggaca tctgctttga caaagttcat tgtgtcgtaa atagccatac ctgctgtaac
    28621 tgaaccacca ggagaattga tataaagata gatatctttt tcagaatctt gagcgtctaa
    28681 gaaaagcaat tgggcgatga tcgaattagc aagatcatct gttacttggc cgcttaacat
    28741 gatgatacgg tctttcaata aacgtgagta gatatcgtaa gctctttcac ctcgtgaaga
    28801 ttgttcaatg actgtaggaa ttaaattcat aatagagttc ctccttgatt tcgtttagaa
    28861 atagtgtacc ataatttagt ttctaatacc taaaatgtag tatacatttt tggtcaaaaa
    28921 aggtcaaata aaaaacttag gcaaattagt cgcataaacc cgctttatgg tatgataagc
    28981 agagaaacaa aacaaggagg aacaacagat tgatagttgg tattactggc cttatcggag
    29041 tgctgacagg tgctgtagtt gtctatatcg gcatgatact gcaaatgcgt cacttggaaa
    29101 ataaagaaca gcgtagacgt gaattggaat tgaaagtcaa agaaatcgag acgctgaatc
    29161 aattaaataa aaagatcaac gagatcttgc aaaaacggac aacgctgatg gaagaatatg
    29221 tcagctttga tgcatttgat gattgttata tcacgattga cgattttgcg tatctccaat
    29281 cattttcagc gcaaaatagt ttttatctgc caaattatat tttagacgag ctatttaaga
    29341 atatctccca tcgaaaagtg attttatctc cagatgaaac aatgaagatt ggtggatatg
    29401 cttataaagg cggacgcgtg attttagaga attttacgga tagtctgact gaaatgatca
    29461 atgaaaaaaa agctcagttg aagactatga cgcgccagcc gattggtttc ttctcgaaag
    29521 atgatgtttg gagctgatat tttgaagcat tgccgttgca catgagagcg tggtaatgct
    29581 ttcttttttt cttaatgata caatagaact gaacggagtg aagaaagatg ttacaaatct
    29641 atgcccatcg cggaagcagc ggcacccacc cagaaaatac gcttcctgca tttgcagaag
    29701 ctgtacgagt aggtgcagat ggtatcgaac tagatgtcca tctaagcaaa gatggttatt
    29761 tgatcgttat gcacgacgaa gaagtggacc ggacgactaa cggtaaaggg ctgattcgag
    29821 aaaaaacatt ggaagaactc aaaaaattga atgcaggggg caaattcagc aaagaatttc
    29881 cagcagctaa agttcctgaa ttgagagaag tgttaggact gctcctccaa aaaaattacc
    29941 gagggatgct aatgattgaa attaaaactg atcagtatga atatcccggt atcgaagaaa
    30001 aattagccga aacattaaaa agtagagatt ggccttttca acatgcatat tgcagctttc
    30061 atttggattc tttggaaaaa atcgcattac tggaaccgga gatacagctg gacttattga
    30121 tgagtacttc tgataaaaag atccaactag cagcagaaac cccttatata gaaggagtac
    30181 atccaaaaaa tacttggttg ttcgaacatc cagtccaggc agctgatttt ccaaaagcaa
    30241 ttcgtgcatg gacgattaac gatgaagaag aaatggccaa atgtcaagaa ctcaaaatag
    30301 atggaataat aactgatttt ccggagaaag ctcttcggtg gaaacaatca acgaaaagta
    30361 ggtaaaatat ggaaaaagta ttagtgatcg tcggtccgac agcagtcggt aaaacagcgc
    30421 taagtgtgga actcgcacaa ctatttggag gagagatcat cagtggtgat tcattgcaaa
    30481 tctataaaaa tctggatatc ggtacagcaa aaatcaaaga agaagagatg aataatgttc
    30541 cgcatcattt gattgatgtg atcgaaccta ctcaacgtta ctcagccgct gattttcagc
    30601 attcaggtcg acaattgatt accgagattg cgaaccgagg gcacctacct atcatagctg
    30661 gtggtacggg tctctatatc caatcccttc tatatgatta tcagttagga gcgacagaag
    30721 aagcgaatcc tgagattaga aaaaagtatg aaacgttagc tgaaacaatt ggttcccaag
    30781 cattatggaa tcagttgaaa gaaaaagacc cggcagctgc tgagaaaatc cactggaaca
    30841 atcaacgcaa agtgataaga gctctggaag ttttagagac gacaggacat agtatcacgg
    30901 cacctaaaga agagccaaag caattgtatg attacctcat gattggatta gatacgaagc
    30961 gtagtattct ttatgaacga atcaatcaaa gagtcgattt gatgctagca gaaggtttag
    31021 tagcagaagc gcgtaaggtg tatgagcttg gagaagtcca ggccagtcaa ggtattggat
    31081 ataaagaatt tttcccttat tttgaaggga aagaaacatt ggaagaagca acagaacaag
    31141 tcaaactaca ttcgagaaga tacgcgaaac gtcagttgac gtggtttcgt aaccgtttgg
    31201 atgcacagtg gtttgatctt ataagtgagc caaatcaaaa aagtcagatt gagcaaacaa
    31261 tcaaaaaatg gctggaggcg gaataaaatg gaagaaaaag tgatcatcgt aggggtagaa
    31321 accgaagcaa accagcgtta cttttcagag tcaatggaag agctagttca gctgacgaat
    31381 accgcatcag gagaagtcgt attcacaatt acacaaaaga gaccacaagt ggatcggcaa
    31441 acgattatcg gtaaagggaa attacaagaa ttagttcaac aagcggatgc gcatgaagcg
    31501 gatttgatca tctttaatca tgagctaact ccgagacaga gccaactgat taccgatgct
    31561 gttggtcttc cggtaattga tcgcgtgcag cttatcttag atatttttgc gatgcgtgct
    31621 cgttcgaaag aagggaagct gcaggttgaa cttgctcagt tagaatacct gctgcctcgt
    31681 ttagtagggc aagggaaaac attatctcgt cttggcggtg ggatcggaac aagagggcca
    31741 ggtgaaacaa agttagaaac agaccgccga catatacgga ataaaatttt tgctgttaag
    31801 aaagaattga aagaagtaga agctcatcga gagcgcaatc gccaaaaaag aaaaaatagc
    31861 gagatttttc agattggact tattggctac acgaatgccg ggaagtcaac tatcttgaat
    31921 ctattgacac aagcggatac ttattcgaaa gatcaattgt ttgcaacgtt agatccattg
    31981 actaagagat ggcgttttgc tgaaggattt gagattactg taacggatac cgtcgggttc
    32041 atccaagacc tgcctacgca actgatcgat gctttccatt ctacattaga agaaagtcaa
    32101 aatatggatc ttttgctaca tgtagtcgat gctagttctc cagatcgtat tttacaggag
    32161 cagactgtct taaaattgat ggatgaactg aatatgaaag aaatgccgat actgactgtt
    32221 tacaataagg cagaccagat cgacccagcg atgttcacgc ctacgctatt cccaaatgta
    32281 ttgatttcta ctcagacgaa agaaggtaag atggcgttga tcgaagcaat caaacgtcag
    32341 ctgatggaac taatgacccc ttatacactt tctgtgccaa gcaacgaagg acagaaatta
    32401 agcgagttga gaagacaaac aatggttttg gacgaagcgt acatggaaga aacaaacgaa
    32461 tatgaagtta aagggtttgc aaaaaccaat tcaaaatggt taagaaaaaa ccaataaaat
    32521 tttatttttt taa
//
LOCUS       DACDIE010000028        32196 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.028, whole genome shotgun sequence.
ACCESSION   DACDIE010000028 DACDIE010000000
VERSION     DACDIE010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32196
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.028"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            6..980
                     /locus_tag="IV250_001428"
     CDS             6..980
                     /locus_tag="IV250_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAP8768718.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTITPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
     gene            1710..2027
                     /locus_tag="IV250_001429"
     CDS             1710..2027
                     /locus_tag="IV250_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8768719.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNASIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(2118..2483)
                     /locus_tag="IV250_001430"
     CDS             complement(2118..2483)
                     /locus_tag="IV250_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8768720.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            2691..3173
                     /locus_tag="IV250_001431"
     CDS             2691..3173
                     /locus_tag="IV250_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8768721.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            3370..4260
                     /gene="pstS"
                     /locus_tag="IV250_001432"
     CDS             3370..4260
                     /gene="pstS"
                     /locus_tag="IV250_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HAP8768722.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            complement(4547..5821)
                     /locus_tag="IV250_001433"
     CDS             complement(4547..5821)
                     /locus_tag="IV250_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP8768723.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSS
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMNSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPRKARLKVKYLV
                     MDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYR
                     RLKRYWKLLLKDSTTWEPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLR
                     YYFVKRGTEGFQETLKTISSTLPQSFKKKFTIFHCYQSGISNAFKVSHSNGVTEGLNN
                     KIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
     gene            6094..8565
                     /locus_tag="IV250_001434"
     CDS             6094..8565
                     /locus_tag="IV250_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP8768724.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HANQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(8668..10404)
                     /locus_tag="IV250_001435"
     CDS             complement(8668..10404)
                     /locus_tag="IV250_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAP8768725.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSVFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(10401..11105)
                     /locus_tag="IV250_001436"
     CDS             complement(10401..11105)
                     /locus_tag="IV250_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8768726.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            11236..11739
                     /locus_tag="IV250_001437"
     CDS             11236..11739
                     /locus_tag="IV250_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAP8768727.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            11699..12037
                     /locus_tag="IV250_001438"
     CDS             11699..12037
                     /locus_tag="IV250_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAP8768728.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            12058..13476
                     /gene="ffh"
                     /locus_tag="IV250_001439"
     CDS             12058..13476
                     /gene="ffh"
                     /locus_tag="IV250_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAP8768729.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            13588..14166
                     /locus_tag="IV250_001440"
     CDS             13588..14166
                     /locus_tag="IV250_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAP8768730.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            14170..14691
                     /locus_tag="IV250_001441"
     CDS             14170..14691
                     /locus_tag="IV250_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP8768731.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(14770..15324)
                     /locus_tag="IV250_001442"
     CDS             complement(14770..15324)
                     /locus_tag="IV250_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP8768732.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            15577..15852
                     /gene="rpsP"
                     /locus_tag="IV250_001443"
     CDS             15577..15852
                     /gene="rpsP"
                     /locus_tag="IV250_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAP8768733.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            15864..16115
                     /locus_tag="IV250_001444"
     CDS             15864..16115
                     /locus_tag="IV250_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP8768734.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            16240..17031
                     /locus_tag="IV250_001445"
     CDS             16240..17031
                     /locus_tag="IV250_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8768735.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            17201..17722
                     /gene="rimM"
                     /locus_tag="IV250_001446"
     CDS             17201..17722
                     /gene="rimM"
                     /locus_tag="IV250_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAP8768736.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            17712..18473
                     /gene="trmD"
                     /locus_tag="IV250_001447"
     CDS             17712..18473
                     /gene="trmD"
                     /locus_tag="IV250_001447"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAP8768737.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            18609..18956
                     /gene="rplS"
                     /locus_tag="IV250_001448"
     CDS             18609..18956
                     /gene="rplS"
                     /locus_tag="IV250_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAP8768738.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(19088..20224)
                     /locus_tag="IV250_001449"
     CDS             complement(19088..20224)
                     /locus_tag="IV250_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8768739.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLHKPDLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKIPETYIVSGINTIDLNNIFEDMIYKDDLSNKYVSVIKSKLNLLFSYAMKKGYVEKN
                     PIDEVVIDYKRESKTIKIKDKFLEDDEYNRLVDFTTSHNKRYSLLFQWLYLTGMRSGE
                     AIALSKDDVHITNNNASVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAI
                     YNELLELNPNGQFLFQTTKGTPFQLTAINTYLRSHKADMKIDKKLSSHIFRHTHISKL
                     AELGTPLYAIQDRVGHENSDITEKIYLHVTKGVKEKLKEDIEKL"
     gene            complement(20340..20906)
                     /locus_tag="IV250_001450"
     CDS             complement(20340..20906)
                     /locus_tag="IV250_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8768740.1"
                     /translation="MAKKVMGQDGKMYKVKKPFYKRVWFWLLAVVVVFIAIGSQGGSD
                     DAKNTVAETTKESVTEVSSAESVAESTVVEEETETTETTIEEVTQEESVPREYRNALS
                     KAESYLGWAGMSEQGLREQLEFEEYPSDAIDYALANVDVDYNEQALAKAESYDDWASM
                     SDSQLYDQLIFEGFTNEQAQYALDNLPQ"
     gene            complement(21042..21695)
                     /locus_tag="IV250_001451"
     CDS             complement(21042..21695)
                     /locus_tag="IV250_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8768741.1"
                     /translation="MKKVSVMLLLSTALLLSACSNNKKAESTDTTSNQETKISKTKET
                     TETSSSTSKSTSKTDSSSTVTSSNQATAEPSPTVISSSQSTIQTAPQEETYEQMKQRT
                     LQSTPADRANWSNKEWEAFGVALYENGLTTDDAGNIISQDQKEQQAASQQNPEDQQTS
                     AQQDADTLSLTDFVNKYGMSPVAWKVQNGMSEEEALRTTQQKTSGEVQLGFSKYGIQ"
     gene            complement(21745..22173)
                     /locus_tag="IV250_001452"
     CDS             complement(21745..22173)
                     /locus_tag="IV250_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP8768742.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRDRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKKIEELKNLYGLPESAYRFLI"
     gene            complement(22178..22552)
                     /locus_tag="IV250_001453"
     CDS             complement(22178..22552)
                     /locus_tag="IV250_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8768743.1"
                     /translation="MSLVTKIKELADEKHVTIAEVERQVGISNGQIRRWDKASPKSEN
                     LKKVADYFGVTTDYLLGNNNVPKWATKEEVVELDKLLDSNVNMSYGGETLTPEQIQRV
                     KDILIATFWDIVKEDKEKGKKM"
     gene            22856..23041
                     /locus_tag="IV250_001454"
     CDS             22856..23041
                     /locus_tag="IV250_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8768744.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            23054..23302
                     /locus_tag="IV250_001455"
     CDS             23054..23302
                     /locus_tag="IV250_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8768745.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
     gene            complement(23605..24063)
                     /locus_tag="IV250_001456"
     CDS             complement(23605..24063)
                     /locus_tag="IV250_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8768746.1"
                     /translation="MIKILSDYQVAITLIISISGFFLSLYNLFKDKRKITLSYFLVKH
                     DRSNRMILTGVIANPSKMPNSIIECTYLYNGKKINCSHYHANGFDMGSIHKSSLLSPI
                     PLAQAISPGYSVAFSEVLDMKDILPGEKLVMVIRTANYQKKFKLKLKDSF"
     gene            24231..24548
                     /locus_tag="IV250_001457"
     CDS             24231..24548
                     /locus_tag="IV250_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8768747.1"
                     /translation="MLIATDTLDRIFLKDEYKLRNIDASGILVFDLYDNGKIGIYQAS
                     DIEETNLAFEQIDDSVELDLDEAILAFEQIAKLLKEAQKNGNSLPTQRVIYQSPGTGR
                     TIG"
     gene            24481..24960
                     /locus_tag="IV250_001458"
     CDS             24481..24960
                     /locus_tag="IV250_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HAP8768748.1"
                     /translation="MATLYQLSESYIKVLELAEQLDEEILRDTLDSINEAIEYKAENL
                     AKIVKEVEGKAELIDSEIKRLQERKTSLLNNAKSIKHYLQEEMEKTGKTKIKGELFNI
                     GIQNNPVSVNVINENLIPKGFFTPVPPKLDKKQLKEELKHGDIPGAELVQTKGLRIR"
     gene            25410..25841
                     /locus_tag="IV250_001459"
     CDS             25410..25841
                     /locus_tag="IV250_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8768749.1"
                     /translation="MKNKLAKTTAIIGLALGSGVIGYAASNAFQDLDTIKANFNTVLQ
                     YGQTKSQRVSELESQLSNNTRTQEQLKAEIEQIKSDKQKEIEAKQREVEQKQQEIATK
                     QQEADSLRQQLSTVQNDKEQLEQRVSELRQYTDQKVGELGK"
     gene            25838..26164
                     /locus_tag="IV250_001460"
     CDS             25838..26164
                     /locus_tag="IV250_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAP8768750.1"
                     /translation="MKELTIYDPNISWAKHTIKVSFMIWGYKGYVTYKVGGNTKGLSL
                     IAIDSDDLYDANFEDNPVNFRDLDEDWFSMELTNDKGDSTLVEDEFDRLGDYIVGVEI
                     IAHEPE"
     gene            26283..26969
                     /locus_tag="IV250_001461"
     CDS             26283..26969
                     /locus_tag="IV250_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012197638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8768751.1"
                     /translation="MQIKKATDIEQTKGTYLIYGAPGKGKTSTAKYFPGKTLILDIDR
                     TSKVLRGEKNVDIVYIDNEDTWNDWGNTLADLTTNYVGVYDNVVVDNVSELERCILSS
                     LGAEGKNNGVPSQGDYQYMQFRMVNSLRYMKNLDSNLIWTAWEEIDLWTDSDGSSYNI
                     ALPQINRKIRNNILGLCDVVGRLMVKEDDERGFILQATDSTYAKNQLDNRSGCKQSEL
                     ILDGIVRLPE"
     gene            26944..28293
                     /locus_tag="IV250_001462"
     CDS             26944..28293
                     /locus_tag="IV250_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP8768752.1"
                     /translation="MELYDYQNDLVDRARQAYVDGYKAPCIVSPCGSGKSVMVAEIAK
                     RTTRKGNRVLFLVHRRELIDQIKDTFQKMGVDNQLVNFGMVQTVVRHLKTIKKPQLII
                     TDENHHGLAASYRKIYEYYSDVPRLGFTATPIRLNGSGLGDVNDLLIEGVSAKWLIEN
                     HRLAPYEYYAPKLIDTAELKKASTGDFTKKSMDKAVKNTIYGDVLKHYKTLAEGEQAI
                     AYCHSIDASKHTAEIFNDAGYKATHIDAKTPKDERADIIEAFRNHEIKILCNVDLIGE
                     GFDVPDCSTVIMLRPTQSLSLYIQQSMRGMRYRPDKTSIIIDHVGNVNRFGLPDMDRE
                     WSLDTKKKTKTDSDLSIVQCAFCFGAYERPKGDNICPYCGELQPIEERKSELEIDESA
                     ELMKVGETKITLNFENNKYYNMTEDEAESIEDLYAIARAKGFKPGWAYMAAKRKGWL"
     gene            28284..28757
                     /locus_tag="IV250_001463"
     CDS             28284..28757
                     /locus_tag="IV250_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8768753.1"
                     /translation="MVVGMGGIFGKKAEDVQGSIINGVTIIGDTGKRDSSGNQIVIAR
                     NKNNEVFESSLSNIKRGVVNGTGEESGYVNLKKANEKYHVEGTYVKALSVGGTKGVNW
                     DKRRNKWKARLYFKGEYVLQKDFNTKEEAIQARLAAEEKYFKPILEKYENKKEND"
     gene            28759..29259
                     /locus_tag="IV250_001464"
     CDS             28759..29259
                     /locus_tag="IV250_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010050472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF669 domain-containing protein"
                     /protein_id="HAP8768754.1"
                     /translation="MTGFNLDFSKAQQGNEIKDGTYEVVVNKAVENATKSGAEFIDID
                     LIVRNDVDQPFQNKHIFAKIWKAKATGKYNEGMIMAIAQALQLEDGKSYNGFDELLAD
                     FVLKTASVRVKTEESNGYKNVNVKSWDKTNTRGVMNHQFKNGDEPSFGPERSRATTVR
                     NDNLPF"
     gene            29274..31586
                     /locus_tag="IV250_001465"
     CDS             29274..31586
                     /locus_tag="IV250_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAP8768755.1"
                     /translation="MYEKIPKELKNLKQWCVYKLVWDEKRQKHTKIPYNANNGHKAKS
                     NDESTWSDFQTALAAIDHYGMSGLGFFFKPPYFGIDIDNAEGEVERFKSGDIEENIIY
                     EFIESMRSYAEYSQSGTGIHIIARGELPGGRRRKGDVEMYQNGRFFVMTGNAASKYLE
                     ITEPNPKDIKRLYDRYVGDEKIIQFKEENPLMNTVDLPVEEIIKRAESSSQGARFKVF
                     MNGGWEAFYSSQSEADMAFANDLAFWTGRDFEKMDEIFRASAMIRSKYDQKRGSATYG
                     ESLLNKAIADTNSVYNPKRKSDFKIFIKDQEQPKEEKYYSYDDTGNADRFTDIYGTLV
                     KYSYIDKSWYYYDGKVWLQDNTGEVRKMIDTTVDIMGKEPLTIPEGADDETKEALMKA
                     KEKHVKRSRSNAGKNAMMDELKHRLSVLPEEFDKDKTLFNTQSGYLSLHDGLLHEHEI
                     DKMFTRVSNVEYTESVDCPMWEEFINQIFDNDQELIRYIQKCVGYSLTGSTREQCMFI
                     LYGHGSNGKSVFLEIISELMGNYAMTMQAQTIMVKQSQSSANSDIARLKGARLVTSSE
                     PNEGVRLDEGLVKQLTGGDKVTARHLYGKEFEFEPEFKLWLATNHKPIIRGTDDGIWR
                     RLNLIPFTVQIPDHKKDKNLKFKLQTELQGILKWAIDGCLLWQREGLEKPKSVVAASQ
                     DYRNEMDQIGTFIETCCETGPGLKISGGELYKVYREWASDNGEHTFTNTKFGREISKK
                     YSKEKAGGFMVYKGITLKPRKYDNVRELFK"
     gene            31833..32150
                     /locus_tag="IV250_001466"
     CDS             31833..32150
                     /locus_tag="IV250_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HAP8768756.1"
                     /translation="MKSEHQIQDEIRIALSKHGCTIFRSNAGQVTTIEGRQFYGMPKG
                     FPDLCGHRNSDGKAIYIEVKNERGKLREDQKRFAEFLNGQPVLYGVARSVEEALKIVE
                     ESS"
BASE COUNT        10681 a         5566 c         6473 g         9476 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtca ctattactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctggactaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgacga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgttggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaacatttt ttcttgatgt gatatccttg tcttttttgt
     1081 aatgttttta acttattgct tttgcaaaat ttatttacct gttttctgga tcaacgatag
     1141 tgtatattat gctatctttg tttggatttt aggacgttta aagaggcact tttttacata
     1201 acttaatcca tagacctttt aagcctgctg tgagataata ataaagaaga gtttaaagcg
     1261 caccccaaac cacttcccca taagtgtatt acgctttaaa ctcttttata tttgaattca
     1321 ctaagaagca taccatattt ttgaaaaaaa gtgagaaaaa aggcttataa ttaggattta
     1381 gagtaattaa ttagatgttt attttatagg actgatatta tagattacaa atagtatcgt
     1441 atttcgcaat cttaaaatta tcttcttact gtgaataaga taataaaatt tgtatttgtc
     1501 ctttttaggt aacttatttt aatctatata cctttctgga tcaacgaaag tatactttat
     1561 atagtcataa cgccgatggt aactatgcac tgtaacgata atgctgctaa aatatctttt
     1621 tacgaaaaac atcacaagga aaaacttaac tatggtatta tctaaacata attttttcgg
     1681 tatttaatat ttaattagga ggtaatattt tgctttttta tagtaatttt atcttaatag
     1741 tagcgatatt attacttctg aatatatgga tttttgatag gagtagaaat gcaagtatag
     1801 gttttagaac taaacgaagt ctgtcttcta aaaaaaattg ggtatactca caaactattt
     1861 tttatggagg aattgtttta attagtttac tttcttcaac actttattct ttgaatatta
     1921 tagatgtttc cacctcaaat tcaatatcta ttattggaat cattattgct gcaattatta
     1981 cacaactgtt tttagtgttt ggagagaaga aaagaagtaa aaaatagaaa aactagtgat
     2041 agttttggtg atataagaaa aacatatatg tataaaaaga agaaagtagc ttctctttgt
     2101 acatatatgg tttgttttta tggatgccga gcttttttat aagataaggc taccgcccat
     2161 aacgacccaa ggatttgtgg aactaagtaa atacctgcaa gaagcaagat cagctgggtc
     2221 ggttttaaag gatgatagaa gtaacgccat tcagtaaaat tggcgcaaaa taaataggaa
     2281 gtgacagtag atataatatt ggcaatgaat aaagatataa tacgcctgtt cctttgaaaa
     2341 taatatccag caaaaagaga taaaaatatc aatgtgatga acccgcttac ggaatgataa
     2401 cgaaaatcaa gcagtaaggc gaaatgcaca taaggaacaa aagataaaag caacggaagg
     2461 tttttccagc aagtttttat catctttcat caccacctaa caagtgttat tattttgtta
     2521 gttttattgt aaattgaatc ctgcaataat caattacttt ttcattattt ctagattaaa
     2581 tgaataaact ctcatgccat tatctctaaa aaaaaagttt ttccccctaa tttttttaga
     2641 tagaaaattg tatagtatga tgagagaaaa agtagtttgg gggaacgtta atgagaagaa
     2701 aagcagtaat cgatcggctt tatttatttt tttggttttt tcctatcgtt ttgctacctt
     2761 ttacatgggt aaatgctgtt gtacaaggtg cggagatatt aggcctgatc caaggaagcg
     2821 gatttgtggt ttctaggaat tttccagctt tgattgtttt ctatttagtg ataagtgtag
     2881 gtctcttatt accaaaatct ctgatcaatc actacaattg gcagctgctt tacttttctt
     2941 gcttattagt atttacatct atgttttctt tgctgcctcg cttgattatc ggccctaatg
     3001 gtttgagcga gagtggattg aaggatttag cttattcctc catgtatttc ttgacgatca
     3061 aaccatggtt ctatttagta attgcctcat ttattcttgc aacatttttt tacttctgga
     3121 cagaaatcag gataaaaagt gaacgatctg tatctataaa aaataactat tgatggtgga
     3181 tcatcagtaa aaaaacatat gagctgcgta aaatgcatgt ttctcatatg ctttttttat
     3241 tattatgtaa agaatgcgaa aaacgtttac atgattttta cataacttaa ctatattttt
     3301 acagcaacca tacgagggat ttacagtcga ctgataaact ttagttgtaa ataagaaagg
     3361 agcaataaaa tgaaaaaagt attgtttact ctaggtatga tgggaattct tttagccgga
     3421 tgcggctcag gatcaggaaa tacagatggc tctgccacac aaaataattc aagtgactca
     3481 aatgaacaag taaaaatcgt tgcggtaggt tcgacagcat tacagccgtt agtcgatgca
     3541 gcacaagaaa gctttgtaca agaaaatcca aattatcaaa tctctgtaca aggaggagga
     3601 agcggaactg gtttgagcca agtcgaagct ggagcagtta ctatcggaaa ctctgatgta
     3661 tttgcagaag aaagagacgg agttgatgct tcaaaattag tggatcataa agtagcagtg
     3721 gtcgggatgg caccaatcgt caataaagat acagatgtca aagatatcac caaacaggaa
     3781 ttgattgata tctttacagg aaaaatcact aactggaagg aagttggcgg gaaagatcaa
     3841 aaaatcaacg tcgtgaaccg tgcaaacggc agcggtaccc gcgcgacatt cgaaaaatgg
     3901 ggactggatg gtgctacccc tgtgcagtcc caagaacaag attcatcagg aacagttcgg
     3961 cagctggtaa gtcaaacacc aggagcaatc agctatttag ctttctcata tctcgatgat
     4021 tccacacaag cactaagtat cgacggtgta gaaccaaaag aagagaatgt agcagataac
     4081 agctggggaa tctggtcata tgaacatatg tacacgaacg gaaaaccttc ccctgaagtc
     4141 caaaaattct tagactacat gatgacagaa gaaatccaag aaggacctgt caaagaattg
     4201 ggctatttac caatcacaat gatggaagta gaacgtgatc acgaaggaaa tatcaaataa
     4261 ctagcagata aacatgccac ggtagtcgtt actattaagg aaaaatcaac tatgtttccc
     4321 cacatataca tttttccttt aattgtgaaa aatagttcct catattttgc cgttttttga
     4381 gctaataccg agggtcagct gattgaacac cgtttattcg aaaaaaagag ctacgacata
     4441 gaactgtcgt aacttttttt ggctcttcgt caaatcgtgt tggtgaagac aaatttgaat
     4501 aaaacttagt ccgatatgcg gcgcgttctg tgcgccgcat atttttttag ttttcaaaga
     4561 tcagaccttg ttgtaaatag atccgtgccc gaaagttata aaagttgcga taaccaaacg
     4621 caattcgttt aatcagcttg atcttgttat tcagcccctc agtgacgccg tttgagtggg
     4681 acaccttaaa ggcatttgaa atacccgatt ggtagcaatg gaagatcgtg aatttctttt
     4741 taaacgactg aggtaacgta gatgaaattg ttttaagggt ttcttgaaag ccttccgtac
     4801 ctcgtttaac gaaataatag cgtaataatt gacaaaaatg atacgcagtt cgcaattctt
     4861 cgttgtagct taacagctca tcaacgattt ctgtttgact aattggtcgt ttaaacagtc
     4921 gatgataatg ccgtttgagt ggctcccatg tcgtggaatc tttgagtaag agtttccaat
     4981 atcgcttgag ccgacggtac ttcttctgat tttcagagtg tgaggttcga aaacgattca
     5041 tgatctggac acgtaattga ttgaagctgc gattgatgtg ttggacaata tgaaagcggt
     5101 cagttacgat ctccgcattt ggaaagactg ttttaagcaa ctgacaatag ctggcattca
     5161 tgtccatcac taggtacttg acctttaaac gggcctttct aggaaagcgc aggaagtatt
     5221 gagttagttt gtaaagtctg cgatcttcaa ggatcgtaaa tagctgattg gttgtcccat
     5281 caacacaaat aaaactcatg gaaccagaaa cagaattcat tgatttgaac tcatccatac
     5341 acaacaccgt cggaaggaag ttcatacgtg gctggtaatg cttcgttgct tcacgcaaga
     5401 tccgtgtcac cgtcccatcc gagacgaagt acttcttagc gattagttta cgtgattgat
     5461 tttcgtacag ttccatgaga atcgcctgct tcagttcctt tgttagttga tgatgttcat
     5521 caactaagtc cgttttcgca gaaaaggtcc ttccacaatc atggcagaga aaacgggtac
     5581 gttttaattc taaaatagtt aacttttctt tgactttcaa catctgagtg cgtgtcgtat
     5641 aggaaccgtt gcggataata cgaaccgatc gacagtgttc acaataggaa gaagagtcat
     5701 ccaattttcc tttaataaac tgcgcttgaa cgcctttata ttctttttca atcagccatt
     5761 cttcatcaaa aaataaatgt ttatctgtta atccgagtaa ttttctagta tgattatcca
     5821 ttgagagact cctttggtag gatgacgttt tggtcgacta aattttacca aaagtgagtc
     5881 tctttttgag ttgaaacgaa gaaaaagtgt tagtagaaaa tcgccttcga ttttccacca
     5941 acacaaaata ttatagaacc cttttttttt gctatatgac aggaatgaaa ggtgtttgca
     6001 agcttaatgt gaggaacact ttccatatct gttctatact ggataggaga agtttggaaa
     6061 tatccagaaa acgaaatcaa tcgaggtagc cacatgaaga ataaatggat aaatcgaagt
     6121 aaaaaaaaga aagtaacaca aagatcatca gataaagtca tccgtctttt tccatcgtat
     6181 aaagaaggat taacggcaga tcaagtcaag gaacgaatag aaaaaggtgc agcaaataat
     6241 tcagtcgatc ccacattcaa aacaaatcag caaatcgtat tggaaaacat atttacgtat
     6301 ttcaatttga tctttcttat cttagccatt ttgctttgtt tagtagaatc ctataagaac
     6361 ttgacttttt taccagttat catagccaat acaggaatcg ccatttttca ggaaattcga
     6421 tccaaaaaga tcttggacga actaaatgtt ttaaatacag caaaagtcaa tgtcatacga
     6481 gacgggatag aacaaacaat agatatagaa gaccttgtaa tagatgacat tgttatttta
     6541 gagactggat atcaaattcc agcagatgca gaggtcgtgg aagggaaact gcaagttaat
     6601 gaggctttat tgacaggcga agcagatgaa ataaccaaag aaataggtga tccattaatg
     6661 tccggcagtt tcattgtttc cggaaaagct tacgcccgat tagacaaagt tggagcagat
     6721 tcgtatattt cccagctgac agtcaaagcc aagacaatgg gagaaggaga acagtcggag
     6781 atgattgctt ctcttaatca attgatcaaa tggattggga tcatcattat tcctatcggt
     6841 atcctgcttt tttctcaaag ttactttttt aatggaaaca cgatcaaaga aagtgtcgtt
     6901 gcaatggaag cagcattgat cggtatgata cctgaaggac tgtacttgct gacgacgatt
     6961 gcattagcat taagtgcgac taaactagca aagcaaagag ttcttttaca caatatgaaa
     7021 agcattgaga cattggcaag ggtaaatgtc ctttgtgtag ataaaacagg aacgatcaca
     7081 gaaaataaaa tgtctgttca aaaaatgatc gtctctaaaa aacaagaaat aatcagtcag
     7141 tcagatcaat tggaagcatg cattgctgac tatgcaaaag cgatggcagg agacaatgca
     7201 acaatggaag ctgttaaagc atattttaca aaaaccactg atcaatccta tcaccatgtt
     7261 attccttttt cttctgtcca aaaattcagc agtgtagcgt tggcagacaa ggtgtatgtt
     7321 ttaggagcgc ccgaaatggt tttgcgcgat caattgccgg aatatgcgga agagtttgtt
     7381 tctttcgctg aaaaaggcta tcgcgtgttg gtctttggtg tgtacgatgg gattcttgaa
     7441 gaaccagtat tgacagaagc agtcacacca ttaggctata ttctgattgc taatcctatc
     7501 agaaaagaag caagagccac atttgattac ttcaaaaaac agcaagtagc catcaaagtg
     7561 atttctggag ataatccgct caccgtatcc aatgtagctg tccaagcagg gatttcacat
     7621 gctaatcagt acgttgatgc atctaaatta tcagaagaag aatatgaagc agctgtcgaa
     7681 aaatatacag tttttggacg cgtcaagcca gaacaaaaga aaatcttcgt tcaattgata
     7741 aagcgtaaca atacagtagc tatgacaggt gatggcgtga atgatattct agccatgaaa
     7801 gaagcagact gcagtatagc gatggcttcc ggaaatgaag ccgccatgca ggcctcacaa
     7861 gtggtcttac ttgattctga cttttcacgc atgccagaag ttgttgcaga aggacggcgc
     7921 gttgtgaaca atattgagcg atcagccagc ttgtttttag tgaagaatat tttctccttt
     7981 ttgctctcac tgttttcagt gatctttgct ctgacctatc ctttagaacc atcacaaatc
     8041 acattgatta gtttgtttac tatcggactt ccatctttct tgcttgctct agaagaaaac
     8101 aaaaagcgaa tcagagggaa attcatcatg aatgtgatgg aaaaagctgt gccaggtgga
     8161 ctaacagata tgatcgtagt cggtgcgttg gtgatctgcg gggtgacttt gaatctaaat
     8221 aaaacagatg tttctacagc atctaccatg cttttgattg cagtagggtt cctcgttctc
     8281 tataaaatct gttcaccgct gaataaattc agaagtcaga tcattctttt ctgtgctagc
     8341 ggtaactttt tttcggtcat ctttttgcat aagctgtttt caatcaccgg tatctcagct
     8401 gtatcacttt tgctgttgag cattctattt ttctcggcag attcgatttt taggcatttg
     8461 accacattgg tagaatattt gtttgaacgt aaaaatgaaa aagagcaaaa gccgtttagt
     8521 cgcgtagcta aaaaatttct atcgatattt aaacgagaaa attgacgaaa agtatctcaa
     8581 aaaagagtaa aaaagaacaa ccactcgtgt atctttcgaa gcaggatgag tcatccaact
     8641 ttaaaacgca cgagcagttg ctctttttta cattttcgga atggtaacgg tgaaagtcga
     8701 acctgttcca agatgactgt ctacagtaat cgtaccatta aggagttgca cgtagtcttt
     8761 tacaatcgct aaccctaatc cggtcccgcc tgaatgtcgg cttcgcgcct tgtctacgcg
     8821 gtaaaaacgc tcaaagatcc gttcctgatc ctcaatgtcg atacctatac caaagtcttg
     8881 gacagaaaac gatagatcat ctgttgcctg atagcggata ataatctcgc tgtcgctttt
     8941 tgaatattgg acagcatttt caatcagatt tttgataatt ggataaagga tatctgtttt
     9001 agtagtaaat ggttcgttct ttccaatcaa tcgaatcgtc aaccgtttct tttcgatgat
     9061 tggctgataa ttttgaacga tttgctggaa ataaggttct gtataaattg tctggatctc
     9121 gtaagaattc tcgccatctt ttgacagctg gatgatttcc cgaatcagtt tatctaaccg
     9181 gatcgcatct ttttgcatga tctcaagaaa agaagtcaga gtttgcgggt catcttttgc
     9241 accatccagt aaagtctcgg taaaaccaat cagtgaagtc acaggagttt ttaattcgtg
     9301 agagacgttg ccaacgaaat ccttttgtag cttttctaaa cgtcgcactt tcgttaagtc
     9361 atacgccacc cctagaatct gtccggtacc ttctgaatta gtaaagtagc gcaaactcaa
     9421 gtctaatgtc tgccgacctt ctgtaagggt gatctcctga tggatcagcg gatgctcagg
     9481 agtcacttga tgaatcagct gaatgagttt tggctcttga atgacttctg tgtaccgctg
     9541 ttcaggttga tagcttacca cgtttaaatg tagctgcatc tttggattaa gcagtagtaa
     9601 acgagaatca gcatcatcaa tcaggaatac gccaatcatc agttcgttca gtaacgtata
     9661 taattgctct tctgtggtag tacacgcccg atacatctta ctcatttgtt cgctcaacgc
     9721 attaatcgtc tgatacaatt cttcccattg atcgggtgtt tgcatgatta cttccgcctg
     9781 attaggattt tgcaccattt tttttagtac gggcagcacg gttttcaatg gctggttttt
     9841 ttgatagatc agataataga tcattcctgc aagaacaagg aagaaagaca gaaagaagaa
     9901 aaagacccaa cggcgaaaac tctcagttct cggtaaaaaa acgcttgtcg gttctgcgat
     9961 acgtaaaatg gcttctaact ggccactttt tttgactggt aatgcgacat ataaaagatc
    10021 ctcattcaaa gtagtacttt tgcgcaaagc gctacctagg ttcccaccat tcaatacggc
    10081 tttgatttcg ggacgggagt ttcgctgttc attcaatgtt tcattgtttg tgtcaaataa
    10141 gatctttcca gtagcatcca ttaaagtaat ccgttcgttc gactgatgga cgaattcttc
    10201 caaagcagtt ttgttttgcg ctgcttggag gttttcgaca tctaattgcc ggatcagcag
    10261 ttgtgctttt tttgtcaggt agctttcttg ctgttccacg atctgctgtt gaaaataatg
    10321 ggaaatcaat tgccagccac caaaaaacaa actaactaaa agaataagaa agaagagacg
    10381 ttcataaatt tttttccatt tcatcgtttg ggttcctgaa acttatagcc aaatcctcta
    10441 atagtcacca agtactttgg gtgttttgga tctcgctcga ttttttctcg caaatgacta
    10501 acgtgtacat ctacgattcg gctttgtcct gcgaagtcaa aattccaaat gcggtctagc
    10561 aatgtgtctc ggtcgatcac acggtctttt cgtttcatga aataaacaag taattcaaat
    10621 tctttcggtg ttagttcaat cagatggtcg tctacggtca cttgataatt ggtcaaatca
    10681 gcagtgattt gtccaatcga tagatattca ggctcagctt catcttttga ttgcttacga
    10741 ggttcaattc gtctgaatat tgctttcata cgggctaata cttcgcgagg actaaatggt
    10801 ttggtcaaat agtcgtcagc acctatttct aggccaatga tccgatccac ttggtcatct
    10861 ttagccgtca gcatcaaaat cggtgtttca tttttttctt gacgcagccg ctgagtgatc
    10921 tccatgccat ccatcgttgg aagcatcaca tctaaaatga tgaaatcaaa tgattgttct
    10981 aatgctaatt ctaatccttc gcctccatct gtggcactgg ttaccttgta gccttctttt
    11041 tccaaattga acgtcaagag cgttacaata gaaggttcat catcaacaac tagtattttt
    11101 ttcattcaaa tctcctttaa ataggtttca ttctgtttat attttagcat agaatcagta
    11161 caaagggcta ttttacaaaa gaaataaatg gtataatagg aaggcacttt gctgaataga
    11221 aaggaagagc agtcaatgat tggaatcagt gcatgtttag gcggtctggc ctgtcgctat
    11281 gatggacaga aaaaagaagt accagaattg atggaactag ttgaaactgg agaagctgtc
    11341 atggtctgtc cagaagtgat cggcggatta ccaattccta gagatcccgc tgaaatcatt
    11401 ggtggaaatg gattcgatgt gtggaaagat caagcaaaag tatggacgac aagcggcgaa
    11461 gatgtaacgg aagcgtacaa aaatggcgcg atcaaagcct atcaaaaatt gcaggaaaaa
    11521 cacattgatc tagtcatcat gaaggaaaaa agcccttcat gcggaaccca ttcgatttat
    11581 gacggaagtt tttccggaat aaaaaaaaat ggtgccggag tggctactgc ttattttatt
    11641 cagcaaaaaa tgactgtgct ctctgatgaa gaatggctga aaactagagg tgagcagaat
    11701 ggaaatcgaa aagaccaatc gaatgaacgc gctctttgaa ttttattcga ccttattgac
    11761 agaaaaacaa atgaactaca tggaattata ttatgcagat gatttttcat taggtgagat
    11821 cgcggaagaa tacgaagtaa gccgacaagc agtatatgac aatatcaagc ggacaagcaa
    11881 gatcttggag gattatgaaa aaaaactcca tcttttttct aattatattg ttcgagaaca
    11941 ggtattggaa aatatgactg catatattac agataagtac ccggaagata aaaaactact
    12001 tgaatatgta cagcaaattc aagcaatcga agaataaata aaaggaatga agaaaatatg
    12061 gcatttgaaa gcttaactga acgtctccag caggcgatgg gaaaaataag aaaaaaagga
    12121 aaagtatcag aagctgacgt aaaagaaatg atgcgggaaa tccgccttgc cttactagaa
    12181 gccgatgtta acttgcaagt agtcaaagat tttactaagc gcgtacgcga acgtgcggta
    12241 ggggcagaag tgctggaaag tttatcacct gcacaacaaa tcgtaaaaat cgttgatgaa
    12301 gaattgacga tcacacttgg ttctgaaaca gcagagttaa ataaatcacc aaaaatacca
    12361 actgttatca tgatggctgg tcttcaaggg gcaggtaaaa cgacctttac tggtaaatta
    12421 gccaattatt taaagaaaaa tgaaaatgct cgtccgctcc taatcgcagg ggacgtatat
    12481 cgaccagctg cgatcgacca attgaaagta ttaggtcaac agctagacgt tcctgtattt
    12541 gatatgggaa ctgaagtcag cccggtagag atcgtccgtc aagggatgga gttagcaaaa
    12601 gaaaagaaaa acgactatgt attgatcgat acggcaggac gtctgcatat cgatgaaaca
    12661 ttgatggatg agttgaaaca aatcaaagaa ttgacgcaac caaatgagat tttgcttgtt
    12721 gtcgatgcca tgactggtca agatgccgtc aacgtagctg atagcttcaa ccagcaatta
    12781 ggtattacag gggtcgtcat cactaaactt gatggggata ctcgaggcgg ggcagcacta
    12841 tcgatccgct cagtgacagg cgcaccaatc aaattcatcg gttctggtga aaaattgact
    12901 gacttagaag tattccaccc agatcgtatg gctagccgta tccttggtat gggggatatg
    12961 ctgacattga ttgaaaaagc gcagcaagac tacgacgaga aaaaagcgga agaacttgca
    13021 cagaaaatgc gggaaaattc attcgatttc aatgacttta ttgaacagct tgatcaagtg
    13081 atgggtatgg gcccactgga agatctaatc aaaatgatcc caggaatgaa tcaagtccct
    13141 ggaatcgaaa acgtcaaggt agatccaaaa gatgttgaac gcaaaaaagc gatggtttat
    13201 tctatgacgc cagcagaaag agcaaatcct gatctgttga acccaagccg tcgtcgccga
    13261 attgctgccg gttctggaaa cagtgttgtt gaagtgaatc gaatgatcaa acaattcaag
    13321 gaatcaagaa aaatgatgca gcaaatgtcc aaaggggata tgaacatccc aggtatggac
    13381 caaatgtttg gttcaggcgt caaaggaaaa ctaggcaaaa tggcgatgaa tcgaatgatc
    13441 aagaaaaata agaagaaaaa gaaaaaacgg aaataagatg aaaaaaacag cttcggtaca
    13501 gactgaaaat gtctactgcc ggagcttttc tatcagcaat ttttattctt tactaataca
    13561 attactctaa acgaagaggt ggcacagatg agatgtccgt ggagtgaaca aacagaaagt
    13621 atgcggcaat atcatgatac tgtctggggt gttcctgaat atgatgacca aaagctgttc
    13681 cgaaaattga tgcttgatat taatcaagcc ggattgagct ggcagacgat tttaaataaa
    13741 agtgaagcat tcgatgaagc atatgatcga ttcgatatca aaaaagtcgc aaattatgga
    13801 gaaacaaaaa tcagtgaact aatggaaaac aaaggaatta ttcgtaaccg aagaaaaatc
    13861 gaagcagccg tccaaaacgc caaagcagtc ttgaaaattc aagaagaaat cggcagtttt
    13921 tcagagtatc tttggtcgtt ttctaatgga aaaatcgttg attcttctta tcattcacaa
    13981 gaagaagtcc caacaacaag tcctctttca gatgaattag caaaagatct aaagaaaaga
    14041 ggatttaaat ttatcggaag cacaacaatc tatgcttttt tagaagctgt cgggatcatc
    14101 aatgatcacc ttgacagttg tttccgcaaa gaagaggtcg tacgtatcgg cagaaagggc
    14161 gaataaagca tgcgaaaaat catattttat ggtgctataa gtttagatgg ctatttggca
    14221 acgaaaacgg atgatcttca atggcttttt gacactccaa caggtaagca aactacttat
    14281 gaagcttttt atgaaacaat cgatacgacc atcatgggca gaaaaactta tcaggaagca
    14341 aaaaaatatt tggagaccga agcaatttat ccagagaaaa agaatttcgt tttttccacc
    14401 cacgcacaat tacaattgcc agatgctgag gtcgtaaacg aagatcctgt tgcttttcta
    14461 aaaaatctac aaatgacaga aggaaaagat atctgggttg tgggtggcgg tcgattgatc
    14521 aaaccgctat tggaacatca aatgattgat gaatggtata tccagattac ccctgtatta
    14581 ttaggggacg ggattccttt attccaaaaa ggaacatatg aagcgcgttt tgaaatgcta
    14641 gacacgacac gattcggtga atttatcgaa ttgcattatg tacgaaagta gaagtaaata
    14701 aaataaaaaa ggcaactata tgcagaaatg tcctgtacat agttgccttt tttattaccg
    14761 ttatgctttt tactctgtta atttgttaga aaaatctaga tacgcatcta aaatcgtttg
    14821 gaagaaactt aatgttcctt caacgatatt accattttca tcaattagat tcgcaacgtt
    14881 tccgatatat gcttccggtt gctgcaaggt tggcacattc aaaaagacaa gtgattggcg
    14941 tagatggtgg tttgcgccaa agccgccgat cccacctggt gatgcagtaa cgactaaacc
    15001 tggtttatga tcccaaacac tgtgtccata aggtcttgag cctacatcta aagcattttt
    15061 caatactgct ggcacagaac ggttgtattc aggagtaacg aaaacgacac cgtctagtcc
    15121 cctcacttcc tcacgaaaac gagtccattc agcaggagcc tgttctggag tttcgagatc
    15181 ctcattgtaa aatggcagat catcgatttt tacaaataca ctttcgtacc cttcaggaag
    15241 caatttcgca aaagcttctg ctactttttt gttgtaagaa tcttttctta aactaccaat
    15301 aaaaaatcca attttttttg tcatacgatt tttcctcctt caaactatct ataataatct
    15361 tacaaaagat aagtaactgg agcaaataaa aacacttact gacaaaagct acatagaaga
    15421 tagattactt atttttaaca aaaaagaagt ctaaaatctt ctattttcta aaaaaaatcg
    15481 aatttaatca agctgtaaag aaaaaaagct ttacagggta aaaaaaaact gttacactac
    15541 atcttgtgaa taaaacaatg gaggtggaaa taaataatgg cagttaaaat ccgtttaaaa
    15601 cgtatgggtt ctaaaaagag tccgttttac cgtatcgtcg tagctgattc acgttctcct
    15661 cgtgatggac gtttcatcga aactgtaggt acttacaatc ctttgaaaga ccctgcagaa
    15721 gtagttttaa aagaagactt agttcttgac tggttatcaa aaggtgcaca accttctgac
    15781 actgtacgta acatcctttc aaaagaaggc gttatgcaaa aacaccacga agctaaattc
    15841 tcaaagaaat aaggtgaccg gatatgaaag atttaagcga cttagtctta acaatcgttc
    15901 gtccgctagt cacttatcct gatcaagtca agttagacgt cgtagagtcg aaagactttt
    15961 acgagtacaa tctaacagtg gctcctgaag atattggccg tatcattgga aaacaagggc
    16021 gtgttgctaa agcaatccgc acaatcgttt acggtgttcg gatcaatgca ccgaaaaaag
    16081 tgcgtttgaa tatcatcgat gataaggaaa agtaaataaa cgtgctgtac tcgaattttc
    16141 gagtgcagct tttttgtttt ttttattaat tgaagaaatg attatttggc aaaacaagca
    16201 agaaagcgta tccttatagg aggagggagt gaacagccaa tgagaaagaa aatgttattt
    16261 attccatttg ctctattgtt aagcgcttgt agttcggtta ctcaagaatc agacaataca
    16321 catctatcaa gcagtatatc tgaaactgtt accagtccag aaaaaagtag taccaaaacg
    16381 gcttccacca ctgaacaaac aatggctacc tcaaataatg agaaaaagac tgctttagac
    16441 cagttgaaag aacaacagcc aaatgttccc atgcctctag atgtaccagt ctcttcaggc
    16501 tatctcaata tcgcagcgac acatacaaag caaggttatt ctattttata ttacagaaca
    16561 gatcgtcccc tcgggttgaa tgctgacgaa ttgaatcaag aaacgcctat tgcaacctat
    16621 ctatatcaat atgggtttgc ttctagtcaa gaaacaatcc aagttttgca gccatttgag
    16681 atcgacacaa atggtcagca agtggactta ggttctcgaa tcacagggta tcagcaaggt
    16741 gcagcaggtt caagttttct tgaatggcaa gaaggaaact ggcgtattcg aatccgtggg
    16801 aataatatag aaggccaaga tcccttacta ttagctaaag aaatcgtagc ttatttggaa
    16861 gaaaacagtc taccagcacc tgaacaattt ggtaaaatta ctgttgatat gggtgatacg
    16921 acgaatcgag ccgtagaagt aagctggcaa gaaccgaaaa atgcctatac gatcactcat
    16981 caagatccta tgtctgcgtt gaagatggca gtttctatga agaggttgtg aaatcagcgc
    17041 gctgatctga gcccatcatt taactccaag tcacaggaag taatcaaaaa tagcacgccg
    17101 catttttgca taattttggc ttaatacctt aaaagaaaac tagaattgcc tcttcacaca
    17161 tgctaaaata aaacagacaa aaaagaaatg gggatataag ttgactgaat acttgaatgt
    17221 aggaaaaatc gtaaacacac aaggtatcaa aggcgaagtc cgtgttatct caacaacgga
    17281 tttcccagaa gaacgttaca aaaaaggaac ggtgctaaca ctattccaag acggaaaagc
    17341 acctgtggaa ttaacagtca aaagtcatcg caaacataaa aattttgact tattgagttt
    17401 tgaaggacat ccttctatca atgatgtgga aaaatatcgg gatggcatat taaaagtgtc
    17461 aaaagacaac tctgtagatc tagctgaaaa tgaattctat taccatgaga tcatcggtct
    17521 gcatgtatta gaagatggaa ctgagttagg taaagtaaaa gaaatcttgt caccaggtgc
    17581 caatgatgtt tgggtcgttc aacgtccgaa aaaagctgat atattgcttc catatatcgc
    17641 ttctgtcgtc aaagaagtcg atttagaagc tggagtggtc cgtgtagaaa ttccagaagg
    17701 actgattgac gatgaagatt gatgtattga ctttatttcc aagaatgttt gaaggtccta
    17761 tgggggaatc catcatcggt aaggctgtag ataaaggatt attagatatc aatatctcta
    17821 acttccgcga ttattcagat aacaaacatc agacagtaga tgattatcca tacggcggtg
    17881 gagcaggtat gctgctcaaa gtccagccca tctatgataa catccaagcc attgaaaaag
    17941 ctgcaccgga tgtcaaaaaa agagtaattc ttcttgaccc agctggtaaa cgattcgatc
    18001 agaaaatggc agaagaattt tcacaagaag aacacttagt attcatttgt ggtcattatg
    18061 agggatatga tgaacgtatc cgcacattag tgacagatga agtttcctta ggagattatg
    18121 tccttactgg gggagaatta ggtgcgatgg tcatgatcga tgcaacagtt cggcttttgc
    18181 cagatgtgtt agggaatcag acttcagctc aaacggattc ttattcaacc ggtttattgg
    18241 aacatcccca atatactcgc ccggcagaat ataaagggat gaaagtacca gaagtattga
    18301 caaatggaaa tcataaattg attgaagaat ggcaattaaa agaatcacta agaaggacct
    18361 atcaacgtcg gcctgatctt ttggaaacac ttgaatggac acctcaaatg acgaaatttt
    18421 tagaagaaat caagaaagaa gaacaagaaa aaacagcaga tgaagccaag tgactttttc
    18481 taaaatcttc tttacagtag gttgataata tgttagtatt ttatggtgag tgcaaaagca
    18541 cttaactatt acgatattcc gctgtgagaa aagctcataa gaatatttgg aataaggaga
    18601 agaaaaccat gaatccatta atcgaagaat taacaaaaga acaattacgt tctgacatcc
    18661 cagcgttccg ccctggtgac actgtgcgtg ttcacgcaaa agttgttgaa ggtactcgtg
    18721 aacgtatcca gttatttgaa ggtgttgtta tcaaacgccg cggtgctgga atcagcgaaa
    18781 cttatacagt acgtaaagtt tctaacggag tgggcgttga acgtacattc ccattgcata
    18841 caccacgtgt tgcaaaaatc gaagtagttc gctacggtaa agtacgtcgt gcaaaattgt
    18901 actacctacg tgcattacac ggaaaagctg ctcgtatcaa agaaatccgt cgttaatcat
    18961 cgttatcaga cagagaaaga acccttggta ttcaagggtt ctttttttat tatctgtttt
    19021 tttatattaa cgatttctat ttggtacatt tttgattaat aaaaaggggc aaaaaagggg
    19081 caacaagtta cagtttttct atatcttctt tcaatttttc ttttactcct tttgtcacat
    19141 gcaaataaat tttttcagtg atatcactat tttcgtgacc aacgcgatcc tgaatggcat
    19201 acagaggagt tcctagttcc gctaattttg aaatatgggt atgtctaaaa atatgtgaac
    19261 taagtttttt atcaatcttc atgtcagctt tatgacttct caaataggtg ttaattgctg
    19321 ttagttggaa aggagttcct ttcgttgttt gaaatagaaa ttgaccattt ggatttaatt
    19381 ctagaagctc attgtaaata gctatcgctt tttttggtaa atcaatttct cgcattccgg
    19441 cagctgtttt tgtagaatca gattttttca tatcagctat tgaacgttct ctatacatca
    19501 tcgtcccatt tataactact gatgcattat tattggtaat gtgtacatca tctttactta
    19561 atgcgattgc ttcaccagac ctcatcccag tcaaatatag ccactggaaa aggagagaat
    19621 atcttttatt gtgtgaagtt gtgaaatcta ctagtctatt atattcatca tcttctaaaa
    19681 atttatcttt aatttttatc gtttttgact ctcttttata atcaataact acttcatcga
    19741 taggattttt ttctacataa ccttttttca tagcatatga aaaaagaaga ttcaatttgg
    19801 atttaattac actgacgtac ttatttgaca agtcatcttt atatatcatg tcttcaaaaa
    19861 tattattcaa atctattgta ttaataccag aaacgatgta agtctcaggt atcttcttct
    19921 ttatagtgtt taatatatta ttcgtaggat aatatgtaga ttccttaact tgtcttttgt
    19981 atataactaa ccattcttca acaagctcat gaaatgttag atcaggctta tgaagtgttt
    20041 tttcgtttaa ttttatatca atttttttat ttaactctag caatgccact ttctgagttt
    20101 ctcgagattt atttttataa gtaatgctca ctttttttct ttttctagtt tttggatcaa
    20161 tatatcgctc attatattta tatacttttt ttccgttttt atcagtttta gtttcgatcc
    20221 acattgtttt catctcctat ttgttgctat aataggaata gataagtaag ccgattatag
    20281 caggtttatt tttcatcacg tccacaaact ttggcgaggg aggggcgtgt ttttgttagt
    20341 tattgtggta ggttatctaa agcgtattgt gcttgctcat ttgtaaaacc ttcaaatata
    20401 agttgatcgt acaattgcga atctgacatt gatgcccaat catcgtaact ttccgcttta
    20461 gccaaagctt gttcgttgta atcgacatca acattagcca gcgcataatc gattgcatca
    20521 cttggatatt cttcaaactc tagttgttca cgcaaacctt gttcagacat accagcccaa
    20581 cctagatagc tttcagcttt gctcaatgca tttctgtatt cacgaggaac actttcttct
    20641 tgagtaactt cttctatagt agtttcagta gtttcagttt cttcttcaac gactgtagat
    20701 tctgctaccg attctgcaga agacacttct gtcacgcttt ctttagttgt ttcagcgacg
    20761 gtatttttag catcatcact gcctccttgc gaacctatag caataaacac cacaacaacc
    20821 gctaacaacc aaaaccatac ccgtttataa aacggtttct taactttata cattttccca
    20881 tcttgaccca taactttttt tgccatttaa atatccctcg cttcttgtta taatatattt
    20941 gtgatctcag aaatgaggta tgagtccgtg ttgcagcacg ggcttttttc tttataactt
    21001 ttttagagat tataggcaaa atagtagggc ataaaaatgt attattgaat tccgtattta
    21061 gaaaatccta attgaacttc accggaagtc ttttgctgtg ttgtacgcaa tgcttcttct
    21121 tcagacattc cattctgtac tttccatgca acaggcgaca tcccgtattt gttaacaaaa
    21181 tcagtaagtg ataaagtgtc agcgtcttgc tgagcgcttg tttgttggtc ttctggattc
    21241 tgttgagatg ctgcttgttg ttctttctga tcttgactga taatattgcc agcatcatct
    21301 gtagtcaatc cattttcata aagggccacg ccgaaagctt cccactcttt gttggaccaa
    21361 tttgcacgat cagctggagt tgactgtaaa gtgcgttgtt tcatctgttc atatgtttct
    21421 tcttgaggtg cggtttggat tgtactctga ctggagctta taactgttgg gctaggttcc
    21481 gccgtagctt ggttggagct tgtaactgtt gaactagaat ctgttttaga tgtagatttg
    21541 ctagtagatg aactggtttc agttgtttct tttgttttac ttatttttgt ttcttggtta
    21601 gaagtggtat ctgttgattc agctttttta ttatttgaac aagctgaaag tagcagagca
    21661 gtacttaaca acaacataac gctaactttt ttcattttat aattcctctt tctcgttgta
    21721 atatgtgtgc taacacgggc ttttttatat aagaaaacga taagcgcttt ctggaagccc
    21781 gtaaagattc tttaattcct cgattttttt aggatattga tcattatctt ctttataaag
    21841 agaaacaatg agattagcag caaagcaatt agcttcgctt tcagatttgc ttctagatgt
    21901 tcttgttgat acataatagc tggataagcc acgatgaaaa atagcgtgac ctaattcgtg
    21961 agcgcaaatg tagaatcttt cctcagagtc tcgcagttca tcatttaaaa agattatcgc
    22021 acgatctcta atttcttgaa actgcccctt gggattctcg ataaaaggaa cgtattgaat
    22081 tttaatgccc atcttttcac aaatataaaa aggattagcg gactggtatt tccgcttcaa
    22141 ctcctcgact aaattaatcg tatccatctc cataagctca catctttttg cctttttctt
    22201 tgtcttcttt cacaatatcc cagaaagtcg ctatcaggat atcttttacg cgctgtattt
    22261 gttcgggtgt caatgtttcc ccaccataag acatattaac atttgagtct agtagtttat
    22321 caagttcaac cacttcctct tttgtagccc acttgggaac attattattt cccaataaat
    22381 aatcagttgt gacaccaaaa taatcagcaa ctttctttaa gttctcagat tttggcgagg
    22441 ctttatccca tcttcttatt tgtccattag agatgcccac ctgtctttct acttctgcta
    22501 tagtcacatg cttttcgtct gctaattctt taatcttagt aactaaactc attattatca
    22561 acctttcaaa gctgaaaaga aaataaatag cttaaaagtt atatttttgg ttgacaatta
    22621 gcttttaagc tagtatattt aattcgtaag ctaaattgtt agctaaataa gagcaacaaa
    22681 aaactctact aatttaaaac attctctcgg tcgccaaact tagaaatgtt attttagagg
    22741 ctttttataa gtcttattta actatgtatt cataatagct taaaagctaa taggtgtcaa
    22801 cgatttagct aattttttag cttacaaatt atttgtttag aaaggagcta tttttatgtc
    22861 tgagaattta gacttaaaaa ttcgagcgga gatgagaaaa agaagaatga ctttcaaaga
    22921 actagctgcg cttatcggta tttcaggagc ttatttatca gatattctaa acggcaatcg
    22981 tgatggaaag aaagcacaac agcatatcga aacagtgaaa aaaatattgg acatccgata
    23041 gggggggtag gcgatgacaa aactaaaaaa acaagatttt gtaaaaaaat ataattattc
    23101 tccatctact tatcaacgtc gaatgtcgga actaaaaaat acagcaattt tctcagcggc
    23161 gtatgaacgg gtcacaggac aagaagtttg gatcaataca gaattatacg ataaattttt
    23221 gtctttcaaa tcctataaca ggttacgcac aagaaaggta acacctaaag aatttatcga
    23281 gaagcattta gttgatttat aaaaaacaga acattttgag aggtgaaggt taatgggtaa
    23341 attcaacaga gcattagtat tcagcgcacc gctaatcgtc tatgctttag ggctttgggg
    23401 aagtagacaa gcgttgatag gaacgattgt gtacatggtt tggattttta tagggctgga
    23461 tgaagctgag tacaaaacaa aaaagccagt cgggagggac tgactaatga gtaaggacga
    23521 tattgtattt ctgttaatta tgttttgtgg gattggatca ataataatta atttgtattt
    23581 gattattaaa gaaaaatttt tcaattaaaa tgagtctttt agctttaact taaatttttt
    23641 ttgataatta gctgttctta tgaccattac aagtttttca ccaggtaata tatctttcat
    23701 atctaaaact tctgaaaaag cgactgagta gccaggagag attgcttgtg ccaacggtat
    23761 aggagataaa aggctacttt tatgaatgct tcccatatca aaaccattgg cgtgataatg
    23821 tgaacaatta attttcttac cgttgtaaag atacgtacat tcgataattg agtttggcat
    23881 ttttgatggg tttgcaatta cgccagtaag aatcattcga ttacttctat catgcttaac
    23941 taaaaaatag gagagcgtaa tttttctttt atctttaaat aagttataga gggataaaaa
    24001 aaatcctgaa atagaaatga ttaaagtaat agcgacttgg taatcgctca aaatttttat
    24061 caagtaattc accacctttt tactcaaatt atatcaaaaa ggagagaaga aataatgcaa
    24121 gaattagtaa ttttgaaaaa taaaaaaaga cacgaacgcc ggcaagcatg atcgtgtcag
    24181 ggaaataaac tatctttcta tattttacca cagaaaggaa tgaaccgtaa atgctaattg
    24241 caacggatac actagaccgc atctttttaa aagacgaata caaactgcgc aatatagatg
    24301 cgtcaggaat tttagtattt gatctttatg acaatggaaa aattggtatc tatcaagcaa
    24361 gtgatattga agaaacaaac cttgctttcg aacaaattga tgattctgtg gaattggatt
    24421 tagatgaggc aatcctagct tttgaacaaa ttgcaaaatt attaaaggag gcacaaaaga
    24481 atggcaactc tttaccaact cagcgagtca tatatcaaag tcctggaact ggcagaacaa
    24541 ttggatgaag aaattcttcg cgatactctc gattcgatta atgaagcgat cgagtataag
    24601 gcagaaaact tagcaaaaat agttaaagaa gtagaaggga aagctgagtt aatcgatagt
    24661 gaaattaaac gtctgcagga acgtaagaca tcgcttttga acaatgctaa gagtatcaag
    24721 cactatttac aagaggaaat ggaaaagact ggtaaaacga agattaaagg tgaattattc
    24781 aacattggaa ttcaaaataa tccagtatcg gtcaatgtaa tcaatgagaa cttaattcca
    24841 aaaggattct ttacccctgt gcctcccaaa ttggataaaa agcaattgaa ggaggaactg
    24901 aagcacggag atattcctgg tgctgaactc gtccaaacaa aaggtttgag gataagatga
    24961 tgaaggagga gaaataatgg aacacgaaaa cgtaaaagac gctttgaaag ctgctatcga
    25021 aatcgcggaa gctaaaggaa taaaggttga cggcaaacca gcaactgtcc atgacattca
    25081 aaatttgacg aaagaacatc tttattttat cgccgacttg cttggtttgt cggaattgta
    25141 tttagacaaa taaccccaca cggaaggagt ggctagggtg gttgtattac aagtaatagt
    25201 ctgcgtaaca ttcatttggt cgtgcatacc ggtttgtata tatatcatga agaagcttcg
    25261 ccctgagtct tacaaattat acgcaattta cagcataaca gtagctcttg catttacatt
    25321 gtttgcaatt tctaggcttc cgttttaatc gtgggaatga atttttaatg aactaaatta
    25381 tctataaagc aaaaaaagga gaaacaacat tgaaaaacaa actagcaaaa acaacagcaa
    25441 tcatcggact agcactaggt agcggcgtta tcggctacgc agcaagcaat gcgtttcaag
    25501 atttggacac gatcaaagca aatttcaaca cagtcctaca atacggtcaa acgaaatcgc
    25561 aacgtgtgtc agaacttgaa tcacagctat ccaacaacac tcgcacacag gagcagctga
    25621 aagccgaaat tgagcaaatc aaatcggaca agcagaagga aattgaagcc aagcaacgag
    25681 aagtcgaaca aaagcaacag gagatcgcta caaagcaaca ggaagccgat agcttgcgcc
    25741 aacaactatc cacagtgcaa aacgacaagg agcagttaga acagcgcgtg agcgagttac
    25801 ggcagtatac ggatcaaaag gtaggggagt tggggaaatg aaagagttga ctatttatga
    25861 cccaaatatt tcatgggcta aacacacaat taaagtttct tttatgattt ggggttacaa
    25921 aggatatgtt acttacaaag tgggcggtaa cactaagggt ttatcattaa tagctattga
    25981 ttcagatgat ctatatgatg ctaatttcga ggataaccca gttaatttcc gtgaccttga
    26041 tgaagattgg ttctctatgg aacttactaa cgataaaggc gacagtacac ttgtagaaga
    26101 tgaattcgac agactaggtg attatattgt cggtgttgaa attattgcgc acgaaccaga
    26161 gtaggagagt gaatacaaaa tgattaaaag aatcactata tcagttgaag aagatagtgg
    26221 atcaacaatg cagcataccg acaatcgaaa aactttttta accaatagaa aggagaattt
    26281 tgatgcagat taagaaggcg acggatatcg aacaaaccaa gggaacatat ttgatctacg
    26341 gtgctccagg aaaaggtaaa acttcaacgg ctaaatattt tccagggaaa acactgatcc
    26401 ttgatattga tcggacttca aaagtcctta gaggtgagaa aaatgtcgat atcgtatata
    26461 tcgacaacga agatacctgg aacgactggg gcaacacgtt ggctgattta actaccaatt
    26521 atgtaggtgt ttatgacaac gtggttgtcg ataatgtcag cgaattggaa cggtgtattc
    26581 tgtccagttt aggcgcagaa ggaaaaaaca acggcgttcc ttcgcaaggc gattatcaat
    26641 atatgcaatt tagaatggtg aacagcctac gctatatgaa aaatttagac agcaacctta
    26701 tctggaccgc atgggaagaa atcgatttgt ggacggattc ggatggatct tcttacaaca
    26761 tcgctttgcc gcagatcaat cgaaagattc gaaacaatat tttgggatta tgtgacgttg
    26821 ttggtcgatt gatggtaaag gaagacgatg aacgcggatt tattttacaa gccacagatt
    26881 caacctatgc caaaaatcaa ttggataata gatctggctg taagcaaagt gagctgattt
    26941 tagatggaat tgtacgacta ccagaatgat ttagtcgatc gtgcaagaca agcctatgtt
    27001 gatggttaca aggcaccatg tatcgtatca ccgtgcggtt ctggaaaatc agtcatggtt
    27061 gcagagatcg ctaaacgaac cacacgaaaa ggcaacagag tgttgttctt ggtccatcgt
    27121 cgtgaattga ttgatcagat caaagatacg tttcaaaaga tgggcgtaga taatcagctt
    27181 gtaaatttcg gcatggttca aacagtcgta cgacatctta aaaccatcaa gaaaccacaa
    27241 ctgatcatca ctgacgaaaa ccatcacggg ttagcagcgt cctacagaaa aatctatgag
    27301 tattattcgg acgttccgcg attaggtttt accgcgacac ccatccggtt aaacggtagt
    27361 gggttggggg atgtgaacga cttgctaatt gaaggagtat cggctaaatg gttgattgag
    27421 aatcatagat tagctcctta tgaatattat gcacctaaat tgatcgatac agccgaatta
    27481 aagaaagctt cgactggaga ttttacaaag aaatcaatgg ataaggcagt gaagaatact
    27541 atttatggcg acgtgttgaa acattataaa acgttagcag aaggtgaaca ggcaatagca
    27601 tactgtcaca gtatcgatgc aagtaaacac actgctgaaa tattcaacga tgctggttat
    27661 aaagcaacac atatcgatgc aaaaactcca aaagatgaac gtgcagatat tatcgaagcg
    27721 ttcagaaatc atgaaattaa aatcctatgc aatgttgatt tgatcggaga agggtttgat
    27781 gttcctgatt gttcgactgt aattatgttg agacctactc aatcgttatc attgtacatt
    27841 cagcaatcga tgagggggat gcgttaccga ccagataaaa cgtcaatcat cattgaccat
    27901 gtgggtaatg tgaaccgttt tggattgcct gatatggatc gtgaatggag tttagataca
    27961 aaaaagaaaa caaaaactga tagtgattta tctatcgttc aatgcgcgtt ttgttttggt
    28021 gcttacgaac ggccaaaagg cgataatatt tgtccgtatt gcggagagtt gcagccgatt
    28081 gaagaacgaa agtcagagtt agaaatcgat gaatcggctg aattaatgaa agtcggagaa
    28141 acgaaaatta ctttgaattt tgagaacaat aaatattaca acatgacgga agatgaagca
    28201 gaaagtatag aggacttata tgccatagca agagctaaag gatttaaacc tggttgggct
    28261 tatatggctg ctaagaggaa ggggtggttg taggaatggg cggtattttt ggtaaaaaag
    28321 cagaagatgt tcaaggttca ataataaatg gcgtaacaat aataggtgat actggaaaaa
    28381 gagatagttc aggcaatcaa attgtaatcg ctagaaataa aaataacgaa gtttttgaat
    28441 cttctctatc taatataaaa agaggtgttg ttaacgggac aggcgaagag agtggctacg
    28501 ttaatttaaa aaaagctaat gaaaaatacc atgttgaagg aacttacgtt aaggctttgt
    28561 ctgtcggtgg aacaaaaggc gttaattggg ataaacgaag aaataaatgg aaagcacgat
    28621 tatatttcaa aggagaatat gtcttacaaa aggatttcaa caccaaagaa gaagcaatcc
    28681 aagcacgact tgcagcagaa gaaaaatatt tcaaacctat cttagaaaaa tatgaaaata
    28741 aaaaggagaa tgattaacat gacaggattt aacttagact tttcaaaagc gcaacaaggg
    28801 aacgaaatca aagacggtac gtacgaagtg gtcgtaaaca aagctgtaga aaatgcaact
    28861 aaatcaggcg cagaatttat cgatatcgat ttgatcgttc gtaacgatgt ggatcaaccg
    28921 ttccaaaata aacacatttt cgcaaaaatc tggaaagcca aagctaccgg caaatataac
    28981 gaaggtatga tcatggcaat tgcacaagcg ctacagttgg aagacggaaa atcatataac
    29041 gggtttgatg aattgctggc ggactttgtt ttgaaaaccg cctcagtacg cgtgaaaact
    29101 gaagagtcaa atggatataa aaacgtcaat gtgaagtctt gggataaaac gaacacacgg
    29161 ggcgtaatga atcaccaatt caaaaatggt gatgaaccat ccttcggacc ggaacgttca
    29221 agagcgacaa cggttagaaa tgacaatctt ccattttaat tgaggtgtaa tcaatgtatg
    29281 aaaaaattcc aaaagagctt aaaaatctca aacaatggtg tgtttataag ctagtttggg
    29341 atgaaaagcg gcagaagcac acgaagatcc cttataacgc caacaacggg cataaagcca
    29401 aatccaatga cgagagtacc tggtctgatt ttcagaccgc tctcgctgct attgatcact
    29461 acggaatgag tggactgggg ttctttttca aaccacctta tttcggcatt gatatcgata
    29521 atgctgaagg tgaagttgag cgatttaaat caggtgatat agaagaaaat attatctatg
    29581 aatttatcga aagcatgaga tcctatgctg agtattctca atcaggtact ggcatccaca
    29641 ttattgctcg cggagaattg cccggcggaa gacgcaggaa aggtgacgta gaaatgtacc
    29701 agaacggccg attcttcgtt atgactggaa atgccgcatc aaaatattta gaaataactg
    29761 agccgaatcc aaaggatatc aaacgcctat atgatcgcta cgttggagat gaaaaaatca
    29821 tccaattcaa agaagaaaat ccgctgatga ataccgttga tttaccggtt gaagaaatta
    29881 tcaaacgtgc tgaatcgtct tcccaagggg caagattcaa agtctttatg aacggcggtt
    29941 gggaagcttt ttattcctcg caatccgaag cggatatggc gttcgccaac gacttggctt
    30001 tttggaccgg tagagacttt gaaaaaatgg atgagatttt cagagcgtct gcaatgatcc
    30061 gtagcaaata tgaccaaaaa aggggatcgg caacttacgg agaatctcta ctaaataagg
    30121 caattgctga tacaaattcc gtctacaacc ccaaacggaa atctgatttt aagattttta
    30181 tcaaagacca ggaacagcca aaagaggaaa aatactattc gtacgacgac accggcaacg
    30241 cggatcggtt tactgatatc tacggaacgt tagtcaaata ttcgtacatt gataaatctt
    30301 ggtattacta cgatgggaaa gtttggctac aggacaatac cggcgaagtt cggaaaatga
    30361 tcgatacgac agtagacatc atgggaaaag aaccgttaac gatccctgaa ggcgcagacg
    30421 atgaaaccaa agaagctttg atgaaagcga aagaaaaaca cgtcaagcgt tctcgcagca
    30481 atgccggaaa aaacgctatg atggacgaat tgaaacatcg actatccgta ttgccggaag
    30541 agttcgacaa agataaaacg ctgttcaaca cacaaagcgg ctatctgtct ttgcatgacg
    30601 gtcttctaca cgagcatgag atagacaaaa tgtttacacg tgtttcaaat gtcgagtaca
    30661 cagaatcggt cgattgtcct atgtgggaag aatttattaa tcagattttt gacaatgatc
    30721 aagaattaat cagatatatc cagaaatgtg tcggatattc cctgaccgga tctaccagag
    30781 agcaatgtat gttcatcctc tacggtcatg ggtcaaatgg aaagtcggta tttttggaaa
    30841 ttatttctga attgatggga aactatgcga tgactatgca ggcacaaacg atcatggtca
    30901 agcaaagcca atcatcagca aacagtgata ttgcacgatt gaaaggtgcg cggttggtca
    30961 cttcgtcaga accgaatgaa ggcgtccgac tggacgaagg tctggtcaaa caattgactg
    31021 gtggtgataa agtcacggct cggcaccttt atggaaaaga attcgagttc gaacctgaat
    31081 tcaagttgtg gctggcaacg aaccacaagc ccatcatccg tgggaccgat gacggcatct
    31141 ggcgacggtt aaatttaatt ccattcactg tccaaatccc ggatcacaaa aaggataaaa
    31201 atctgaaatt taaattgcag acggagttgc aagggatcct taaatgggca atcgatggtt
    31261 gcctgttgtg gcagagagag ggcttggaga aaccaaagtc ggttgttgca gcgagtcagg
    31321 attatcgaaa cgaaatggat caaataggta catttatcga aacgtgttgc gaaacaggtc
    31381 caggactaaa aatatcaggc ggagaattat acaaagttta tcgtgaatgg gcatcagata
    31441 atggcgagca tactttcact aacacgaagt ttggcagaga gatttcaaag aaatatagca
    31501 aagaaaaagc tggtggattt atggtttata aaggaatcac attaaaacca agaaaatacg
    31561 ataacgttag agaacttttt aaataaatct ggagggttct ggagggttga gaacgaaacc
    31621 ctccaaccct tagaaacgtt gatatatcag tatttatatt atatttttct ttctttctgg
    31681 agggttttaa aaaaataata aagtattaaa gtaaaaaata aaaagtaaat tatataaaaa
    31741 agaataggtt tttgggtctg accctccaaa ctctccacca aaatcttcct aaatgctttg
    31801 ataccaacga ttaaacagtc tggagggtta cattgaaatc agaacatcaa atacaagacg
    31861 agatcagaat tgccttatca aaacatggtt gcacgatttt tcgatccaat gcgggacaag
    31921 tgacaacgat cgaagggcgg caattttacg ggatgccaaa agggttccca gatctatgtg
    31981 gacatcgtaa ttctgatggg aaagcaattt atatagaagt aaaaaatgaa cgcggtaagt
    32041 tgcgtgaaga tcaaaaaagg tttgctgaat ttttaaacgg tcaacctgta ctatacggtg
    32101 ttgcacgatc agtggaagaa gcattgaaaa ttgtggagga atcatcatga caacagaaga
    32161 agtgattcaa atgcgtattc gaagccttca gcgtga
//
LOCUS       DACDIE010000029        31499 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.029, whole genome shotgun sequence.
ACCESSION   DACDIE010000029 DACDIE010000000
VERSION     DACDIE010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31499
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.029"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(811..1152)
                     /locus_tag="IV250_001467"
     CDS             complement(811..1152)
                     /locus_tag="IV250_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP8768757.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(1152..1382)
                     /locus_tag="IV250_001468"
     CDS             complement(1152..1382)
                     /locus_tag="IV250_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP8768758.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            1669..2007
                     /locus_tag="IV250_001469"
     CDS             1669..2007
                     /locus_tag="IV250_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8768759.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(2212..2451)
                     /locus_tag="IV250_001470"
     CDS             complement(2212..2451)
                     /locus_tag="IV250_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HAP8768760.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(2427..2774)
                     /locus_tag="IV250_001471"
     CDS             complement(2427..2774)
                     /locus_tag="IV250_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF159 family protein"
                     /protein_id="HAP8768761.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
     gene            complement(3506..4087)
                     /locus_tag="IV250_001472"
     CDS             complement(3506..4087)
                     /locus_tag="IV250_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8768762.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(4270..4896)
                     /locus_tag="IV250_001473"
     CDS             complement(4270..4896)
                     /locus_tag="IV250_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP8768763.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(4918..6249)
                     /locus_tag="IV250_001474"
     CDS             complement(4918..6249)
                     /locus_tag="IV250_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HAP8768764.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(6404..6805)
                     /locus_tag="IV250_001475"
     CDS             complement(6404..6805)
                     /locus_tag="IV250_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP8768765.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(6891..7097)
                     /locus_tag="IV250_001476"
     CDS             complement(6891..7097)
                     /locus_tag="IV250_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8768766.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(7192..8694)
                     /locus_tag="IV250_001477"
     CDS             complement(7192..8694)
                     /locus_tag="IV250_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAP8768767.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(8712..9167)
                     /gene="argR"
                     /locus_tag="IV250_001478"
     CDS             complement(8712..9167)
                     /gene="argR"
                     /locus_tag="IV250_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP8768768.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(9265..9882)
                     /locus_tag="IV250_001479"
     CDS             complement(9265..9882)
                     /locus_tag="IV250_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8768769.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            10103..11476
                     /locus_tag="IV250_001480"
     CDS             10103..11476
                     /locus_tag="IV250_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP8768770.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            11551..12663
                     /locus_tag="IV250_001481"
     CDS             11551..12663
                     /locus_tag="IV250_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAP8768771.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(12735..14372)
                     /locus_tag="IV250_001482"
     CDS             complement(12735..14372)
                     /locus_tag="IV250_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAP8768772.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(14540..15148)
                     /locus_tag="IV250_001483"
     CDS             complement(14540..15148)
                     /locus_tag="IV250_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8768773.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(15532..16683)
                     /gene="queG"
                     /locus_tag="IV250_001484"
     CDS             complement(15532..16683)
                     /gene="queG"
                     /locus_tag="IV250_001484"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAP8768774.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(16702..17454)
                     /locus_tag="IV250_001485"
     CDS             complement(16702..17454)
                     /locus_tag="IV250_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8768775.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(17595..18545)
                     /locus_tag="IV250_001486"
     CDS             complement(17595..18545)
                     /locus_tag="IV250_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP8768776.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            complement(18546..19385)
                     /locus_tag="IV250_001487"
     CDS             complement(18546..19385)
                     /locus_tag="IV250_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP8768777.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(19382..20128)
                     /locus_tag="IV250_001488"
     CDS             complement(19382..20128)
                     /locus_tag="IV250_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP8768778.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            20366..21736
                     /locus_tag="IV250_001489"
     CDS             20366..21736
                     /locus_tag="IV250_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8768779.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(21791..22687)
                     /locus_tag="IV250_001490"
     CDS             complement(21791..22687)
                     /locus_tag="IV250_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8768780.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(22968..23645)
                     /locus_tag="IV250_001491"
     CDS             complement(22968..23645)
                     /locus_tag="IV250_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP8768781.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(23736..24641)
                     /locus_tag="IV250_001492"
     CDS             complement(23736..24641)
                     /locus_tag="IV250_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP8768782.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            24816..26270
                     /locus_tag="IV250_001493"
     CDS             24816..26270
                     /locus_tag="IV250_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8768783.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVVPSH"
     gene            26286..27317
                     /locus_tag="IV250_001494"
     CDS             26286..27317
                     /locus_tag="IV250_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP8768784.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            27324..28511
                     /locus_tag="IV250_001495"
     CDS             27324..28511
                     /locus_tag="IV250_001495"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAP8768785.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            28667..30049
                     /locus_tag="IV250_001496"
     CDS             28667..30049
                     /locus_tag="IV250_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP8768786.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            30176..30406
                     /locus_tag="IV250_001497"
     CDS             30176..30406
                     /locus_tag="IV250_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8768787.1"
                     /translation="MTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGE
                     AYEDYFMIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(30606..31274)
                     /locus_tag="IV250_001498"
     CDS             complement(30606..31274)
                     /locus_tag="IV250_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAP8768788.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
BASE COUNT         8896 a         6385 c         5690 g        10528 t
ORIGIN      
        1 attgtaaata ataatattga caaaaagtga ctcaaaataa aaaaaaactt acattatact
       61 ggaatgaact gtattgatag tgtacctaaa agctctactg acataagatc gtgtaatcgg
      121 taaagtaatt ttattaatta tacgggatgt aatcaattag agaataaact aatcataaaa
      181 ggtactttta cattcataat tgtcgctttg agattctact ttggttcact atctgaccca
      241 attgcacatt ttcgatcatt acaagtattg gtgtcgttag aacaactaaa taaaaactag
      301 cggtagattt ttcagaatct atgctaattt ctgtatggat gttccagtga tggcaaattt
      361 ctcttttcat tgtttttctt gtcgcatgat atgccttttt gtgttattca gttaaactat
      421 agaaattctt tcctagacag atcttgatcc caaattttac gtttttgagc atcgatatag
      481 tttaaaatga cattactcat atctaaagag tttagatttt ttaggactaa aactgtcagt
      541 tgttcttttt cttctttctg ttaaagtatt gtcgcgactt ttcgtatacg ttaagatatg
      601 tcgtgaaaaa acaagttttg tgacacaaaa aaacgaatga cttgaggaaa tcacctcaat
      661 cattcgttta aaatgctata tattctttag tttaagatac ggaaattaat gccatcgacc
      721 acactagtag aatgaacgac atttacttgg ccttttttta atttcgttat gtttagcata
      781 taaaaatatt atagagttta cttacaatgc ttaaaacata tactgtataa tctgatcaat
      841 tttagccatg tcttgcaatg gtaagttttc tatttttttg agttttcgtt ccttaaaatc
      901 aagagatttt aattgtgtaa taattacttg tccttttata tctagctctt caggtaatga
      961 gtaacgtgtc ggtaaattct ttatagtgga agtaattgga catatgaccg aaaacattgt
     1021 tttacggtta aattcataac gagagacgac tagtcctggt cgtctttttt gaatttcttt
     1081 tcctacggag ggatcaaaat caatccatac aatatcacct tttttaggaa tataaattct
     1141 ttcttctgac actataaaat ttctctccct tcaggagtta aatcttccca ttcgtctttc
     1201 tcataatact cggcctcttc cccaccacta aatggatctt caatttttgg aactaacgta
     1261 attgttccat catcagaata cacaacgata tattcttggt tttctgaagg tttctttccg
     1321 ttatcagaag gtaaagttac cactacagaa cttccttgta atctagattt tgtaacaagc
     1381 attatcatca ctcccttaat aaaagtatac tatataacac taagtaacgc aaaattcccc
     1441 tttgtaaact atctagaaaa ttgttttgtt taaagtatat cttattggat ataaatagaa
     1501 ataaattgta gccagttcat tttttcttta actaaaaaaa taatccaaga aacattgaac
     1561 ctatggatcc aaaagctacc caaatactat agctaacccc ccgctacctt caattttgca
     1621 gataaaacgc tttgggagaa aatttcaaag acaacaggcc atcctatcat gcccgattat
     1681 aaacaaaaag ctggctattc cttttgctct gacaaatttc aattgggaaa gttcacgaaa
     1741 ctagcaggtg aagaagtttc aactgttcgg atcaaagaat gcccgcttca aatagaaaca
     1801 ttggttcaac gaatttcaac tagacaagac tttgctattg tagaacgtga gatcgtagct
     1861 atttttgtag aagaagggat tttacatgat ccaactcata tcaatgtgga taaatggcaa
     1921 cctcttatct ataaattcag agaatataca actgccaacc agtcgttaac tttgaatttt
     1981 agattccaag aattcaaaga gtcataatga taaacatcgt taggtataaa taaaaaagac
     2041 tcgcagcacg agtaaaaaac aaatagaagc tatacattag aaaggtcgac tgtgacccta
     2101 tagatgcagt atatgcagct tatgagcatt tcttctgttc tctttgtttt tgggatctgt
     2161 gctgaagtct ttttcgtttt ttgccgctct ttctttgaaa aatcgattat gttatacctt
     2221 ctccattact aattcaggca tctgctgagt aatgattttt cgcgcaaaag aaatatctgt
     2281 tacccaaggt tcgatttgtt cttcagtcaa gacaagcggc attcggtcat ggattggtgc
     2341 gactgtgcgg ttaggttcag tagtcattat gattgattct gccatttttt catcctgatg
     2401 gctacgataa aaaccgccaa tatacactat tttttcctta tttgaaaaca taaatttgtc
     2461 tttttcttca ttccactcat tgggaaaaca caacgatttt gcaaaaatgg ttgatgaaag
     2521 gtcttttttt cttctactgt ctctgcacgg gcattaatca tcaattgtcc ttttttaaat
     2581 ccggtaaatc cccacttcat gatttccgga gtgaggatgt tttcttcctc ttttcttaac
     2641 gtcacaacaa gatttgacgg aaaaacttct ccagctgcta attctgctgc ctgcttcggt
     2701 tgtttcgtct gggcttctcg ataataattc tgaaggagct ctgactctag atcaaaaaaa
     2761 taacgaccac acatcctaat ccctcccaat ctttttttct atcatacact gcaacaagaa
     2821 gttcgcacca acaaaacgct ttcaattttt gtagaactaa aaaagttgtg acaagtgaca
     2881 aactctccag tgaatggtat tctcctgcca actcctcggt cacaagtcac aatattcaac
     2941 aaacatgaga agctgctttt tgaaattgtt ccttgtgact cggagtgaaa cggcaggctt
     3001 acgacctctg ggtaaacggt gttcaaaagc cagtttctcg gtattaagcc aaatttatgc
     3061 aaaaatgtag aaagctattt tcacaaaatt tttcttaata ctcgaaacta agcgactttt
     3121 tcacaacctc ttccttaaac ggtgttcaaa agcttgcatc tgcggtaata agcccaacca
     3181 aacgaaaaat atgagcagct atttttattt ggctgttctt attacttgat gcagggcagc
     3241 tttttcacaa cctctccact aaacgatgtt caatcagcta accttcggta ttagctcaaa
     3301 aaacggcaaa atatgaggaa ctattttgcc gtttgtttct ctaatactca ggtcaaagcg
     3361 ctgatttcac aacctctaat acacggtgtt ccagaagtag cttcttcgaa aaataagccg
     3421 aaatttcaca aaaatttgag aagcaatttt cgtaaattcc ttcttatttc ttgaagctga
     3481 ccacttctgt cacaacctct aattattaaa gaaccttttg cgaagttaac aatctttctt
     3541 tgatcaattg aaaattctgc ctaagtagtg atagatcctg tgtgactttc aacaataaga
     3601 tacttccctg gatttcagca atcaacgctt tggcttgacg gtctgcttct tcatcggaca
     3661 gtttttcttc agaaagtgcc aggatatttt gtttcgtata cttgacccat tgattgacta
     3721 attctagtag caacagacgg tattcttcat ccactgtact caactcaata atcaaattac
     3781 ctacaggaca gccatgataa gtgatctgtg tctcatgatc agcgaaaatc cagtccaaca
     3841 tttcttctaa tgcttcttct ggctgttttc cagcatttaa gatgtcttcg aacaattctg
     3901 tctgccatac gtccatatgc gcatggatca ccgctagaca aacttctttt tttgaagaaa
     3961 agtaataata aaattgacct tttcctgtat cagaagctgc tagaatatct cggattgacg
     4021 tttcatgata gcctttttta taaataagat ctttggtcgc ttccaagatc ttttcctttg
     4081 ttttcaatga tattctcctt tttcatttct catagtccta tattatacag taaattcatc
     4141 attttttctt cttaatcgtt taaaaaagac tgtgccaaat tatctgacac agccctcact
     4201 ataaaactgt tctcatatag ctagatgttc attgattcaa gaactgcgag gacaaaagca
     4261 attcctatct tatgatagtt gctctaatac ttcctctgtt gtcatcactt gatctgcaat
     4321 aaagccaaag ttcgtcaacg ctgcatccat tgcttcttgg ccaggagcac caattgcatc
     4381 tgtcgctacc acaacattga atccactctc taccaattca cgcatatggg aatcgctaca
     4441 caagtttccg ttcattccac caagaattac tgtgtcgatt ccattttttc tcaattggag
     4501 cgccaaatca ttgctttctg gtccaaagat tttatgagga gagctgatga tcgtattttc
     4561 atccaaataa ggtttcaatt cttcgataaa atcggctcct gaaccttctt ccactgattc
     4621 atattgactt tttctagcaa acatctgctg ctcaagcatc atatgttctc ctgcaccgcc
     4681 aaattgccat ttatggtcat gtgggtaaaa ataatgagga gaaataaaga gtttgtatcc
     4741 tttttctttt gcttgtttta ccatttcaat catattttcg atcgtattgt atttttctaa
     4801 gatatctttt gtcaaaccaa agccaccgcc tgctggtttc aagaactcga cttgcgggtc
     4861 agtgagaact actgctgtat ttttattatt gaacatggtg gattctccat tcgttattta
     4921 ttttaataca tcatttagag cttcactcat tgtcggatga gaatagattt gatctctcaa
     4981 taacgtataa ggaagttttc cattcatagc tagcgaaatc aagttgatga cttcatgaga
     5041 atcttccgca tagatacttg cccctagaat caagtcagtt tcagggtcga ctaaaacttt
     5101 gaatacacct ttaggatctt ctaatacttg tgctttaggg acaccgcttg tcatatactt
     5161 gaataatttg taattgacgc cttgtgcttt ggcttgtttt tctgttaaac ctacattgga
     5221 taaagtcgga gtgataaata cactgtaagg cacagctttc cggttattcg ttgttcgttt
     5281 attctcgccc ttcaactgat ccataatgat tcgataatca tctagagaga tataagtgaa
     5341 ctgcaatccg cctttgacgt ctccaatcgc ccaaatgttg tctgcatctg tacgaaggta
     5401 ttcatccaca gcgattgctc cgcgatctgt tagtttaaca tctgtatttt ctaatccaag
     5461 tgatgctgta cttggttttc ttccagttgc agccaaaact tttgacgctt ggattgttac
     5521 ttcttggcca tcttctgtaa aggtgacggc agctgttgat tcttggtctg ctacttgatc
     5581 tacagataca cctagatgga attcgattcc cgcatttgtc atgtcttcta aaatcatttg
     5641 cgcgatatct tcatcttcac gtggaagaaa ttcagaatgg gcatcaagaa cgacaacttt
     5701 agatccgtat tcattgaaca ttgatgcaaa ttccaagcca atatatccag ccccgatgat
     5761 cactaatttt tccggtaatt gttcctgatc catcgcttgc gtagaatcaa tcaagtagcg
     5821 acttgtattc aatcctttga ttggtaaaat gattggttca gaaccagtat tgatgaaaat
     5881 ccgttcgcct tcaacaatca ctttttcacc attattcgtc acttcgattt gatggttaga
     5941 aagaaaacgt gctgtaccgt ccatcactgt cccagttgcc tcatcgctga tcatatgata
     6001 atttttggcg cgaagcatgc ccgtcaattt ttctttccga gcaacagcct ctgtaaagtc
     6061 gattcctctt tctccattga aaatcaatga tttagaagga atacagccga tgttgataca
     6121 tgttccgcca tacatacgtg ttgacttttc aacgactaaa accgattcac cttttgaagc
     6181 taacgccttc gctaaagtct taccgccctt tccaaaaccg atcacaatat tttgatattt
     6241 ttccatcatt tttccctcgc ttctttttta cctaccgatt ggtacaaaaa tatgataacc
     6301 gataattgtc tattctccaa atcatctgct ttatttgtca aaaaaaacca gacaaaaagc
     6361 ggtcctttca cgtttttagt ctggttcgat ttcctttatt cttctaaaca atcgttactt
     6421 ctacatcgat atttcctcta gttgctttgg agtaagggca gaattcatgc gcatcttctg
     6481 caattttttg ggcttcttct tcgctgatgc catcgatatg cacttctaat actacaccga
     6541 tctgatagcc gccatcatct aaattataaa gagagacacg tgcttttact gtggactctg
     6601 ctgtcattcc tgcttctttg atcatatgtt gtaccgcccc attgaaacaa gaagcataac
     6661 ctgctgcaaa taattgttct ggatttgttc cttctgcatg gattcctgga ggagtgattt
     6721 tcatatgcat cgagccattt ggtgcctcga cttctccatt gcgcccgcca tggttgatca
     6781 ttgctgtttc gtatgctttt ttcattgtac tgctcctttc gattgaccat ttgctacttg
     6841 gctataatta tagtgtatga caagtaaggc gctttctaca aaaattctgc ttattcgaat
     6901 accagaatgc cgtcttctac tatttttcca aaaaacaaaa aaatactact cgtgaacaac
     6961 agcgtcatat ttgttccaaa ataaatagca atcttttcta cataatcttt tatatgattg
     7021 attttaagat acatatatac cagaaagttg accccataaa ccaatactgt taaaaaaata
     7081 accatattcg ttaccatttt ttcctccttt tgctggtctc cctaccagca ctcgatttaa
     7141 ccataccact ttttttgccg tccaagcaac tagcctttca aaaagactgg tttattctgc
     7201 tgttaatgat tcaagatcag aaaaatgtcc gaataatttt gctaatttta ctaacgccaa
     7261 cttgttcttt ccgaattcat cattttctat ctgtaatact agtaacggct catgtaagct
     7321 taaacggatc aaaaaccagc cgtttccgta tggctcttct agatcatatc gaataccttc
     7381 ttcattttcc gggtcgaagt tcatcccttt ttcttcttcc aacagtcgct tcatttcttc
     7441 taatatcttg tttccttgtt cgcgtggttc atcgctgaac aattttaatc ggatttcttg
     7501 tacttctacg ggctgtttca actctttgat ttgttcatct aatgtgatac cttttctttt
     7561 taattctggc aaaagcatca ggatctttgc gatgacatat gcaccatcat ccagaaaata
     7621 gttttccttg aatgcagcgt gtccgcttgt ttcgatggct agctgtgtat ctttcccaag
     7681 acggttaagt tctaatgctt tattgattac atttcgatag ccggaaatat aacgaacttg
     7741 gatgcctcct ttttcttcaa tgaatctttg tagatgagaa gaagtagggg aattcgtcac
     7801 gattcttgtt cctggatgct cttttaatac gattgtactt aatactgcga tcaaattatt
     7861 tcgattgatc actgttccag aagcactgac aactgcagaa cgatccacat ctgtatcgaa
     7921 aatgatccca agatctgctt catttgctaa aacagcctgc ttgatacttt ccatcgcttc
     7981 tttattatca ggatttggga tatgattagg aaatcggcca tctggttcca aaaattggga
     8041 acctgttgaa tccgcaccta agctattcaa tactttttca gcaaagaatc caccagcacc
     8101 atttccggca tcaacaatga ttttccaacc atttagtggt ttgctttcta gtgttccaat
     8161 ccccttttta atttttgtga ctaaatcttg cgcataaggt gtcaaaaggt ctgctttttc
     8221 tacgcttcca gtttgttcgc tttttatttt ttgctgctgt gataggataa aagcaatatc
     8281 ttctttttct gctcctcctt ctgaggtaaa gaattttatt ccattataat aatagggaag
     8341 atggctagca gtcagcataa ccgcagcatc gcacgagaac tgttcaaatt gagtggacat
     8401 aaacatagca ggcgtagtag ctaattcgaa atcgatgaca tgtatccctt gttctttcag
     8461 actttggatc aacgcctctt tcagtgctgg tccgcttatc ctactgtcat gaccaatccc
     8521 aatcgtccat tttttatttg gatcggttga tttcttcttt ttctttatcc agtttgctag
     8581 tccccagccg atctttttta ccatgtctgt cgtcaagttt acttcatatc cttcaactgg
     8641 taatgcgatt cctcgaatat ccgatccatt ttgcagcgct ttcatatctt tcatataaaa
     8701 gtcctcctca tttattccct cattttttcc agcttttctg caaatcgctg tgcttcttct
     8761 tctgagcggc aaataataat gaatgtatct acaccggcca tcgttccgac aacttcaggg
     8821 taagccactt catctagcca attcgttacc acatcggcac catttcgttc agtcaatacg
     8881 ataaccatga actgaacaga ctggatacgc aaaacctcgc gtctaattgc tgtacgcaaa
     8941 cgttcttcat ttaaagaaac agatggctgg ttaaataatg tatagcgtac tattttgttt
     9001 tcattttgac tttttactaa cttcatttct cgaatgtctc gagaaatcgt tgcttgagtc
     9061 acatcggcgc ctgcttcatg cagccgcgca agcagctctt cctgtgtccc aataggttgt
     9121 tctgtaatga tctggcggat cagtgcttgt cgctgtttct ttttcatatt agacacctct
     9181 caatgttgat tatagcaaaa gataagtaat atttcttcat attttggtat tttttacttt
     9241 tattttttgt tttcattcag gttgctaacc tttttctatt ttaacacgca aaatcgtctt
     9301 cattttttca ggtgctgatt ttcttggatc gctgatgtaa atctctttgt gttctttaga
     9361 agctctttca tagccttctt ttgcgatgaa tgcttctaat ttctcaaacg ttgctggttc
     9421 ttcggcatag ggcccgacgt gtaagatttg ggcacaaaga ccttcttcca tctttttgaa
     9481 tactacttgt tctttcaagt cttccggaag tttgctagaa cgctgaatgg cttcttgtgc
     9541 tatcgcttct gtaacaaagt caggctgtcg gatcatcaat tgataagaaa aatgttcttt
     9601 tttcatcact ggttcatgca aatacttttc ttgcagtccc cattgtcctt ccagtggata
     9661 gacagtatac atctgataat caggaatcag ccaatttttt ttaggcgaca tgcgtattgc
     9721 atagctgact gcatatagac aggctactct tcgtttaaag tcttcctgat tcgggtcgcc
     9781 gatccctctg atgctaaaga atgatttttt aggaacctct agtatttgcg ggcttttttt
     9841 catactatag ctcttgtctt tacgccattc aattttttcc atttttcttc ctccgcgaaa
     9901 ttccatttag ttatttatta tcatacctct ttttgagtga cttggctaat cgtgttgaca
     9961 ctatttttca gtttgtgcta gaataagtga agtaaataaa tcgtacagat aacacgtttc
    10021 ggcgtgtcga gagagaggcg gtttctattt ttataataga aatcactctc tttttttacg
    10081 aaatttttta gaggtgatgt aaatgacaaa acaaaaacag ctgcttttag tacctgcagt
    10141 aattgcaaca gggatgatgt cttttgcggg cgtattgatc gagacggcca tgaatgtaac
    10201 tttcccaact cttatcagac agtttgattt aacgactgca caagtccaat gggtaacaac
    10261 gatttatctt ttgatgattt cgatcattgt tccgctgtct acttatttga atcgcaattt
    10321 tagtctcaaa aaactctttt tagcttcaaa tctgcttttc ttggcaggta taatgatcga
    10381 tttcttctct cctaccttca tggtcctatt gtttggtcga ttgctccaag gtgcagcgac
    10441 aggtatcgct cttccgttga tgttccatat tatcttgaca tttgcccccc tcaatcgacg
    10501 aggcgcgatg atgggtgtcg gtactttgac aacggccatt gctccagcaa ttggccccac
    10561 ttatggaggc attatgactt cacattttac atggaatcat atcttcctgt ttcttgtgcc
    10621 cgttttgttt ctttctttga ttatcggctg gtttgctatt cccgagatgg cagtacaaaa
    10681 aagtggttat ttggattgga taagcgtttt aggaatttgt cttttgttta gtggaagtct
    10741 aacatttttg agtacgttgg gaacagtttc cagctggatt gctttaatca tcggcggtat
    10801 tggctggttc cttttttact atcgaagtaa aaaagtggag catccactga tccgactgtc
    10861 tattctttca aataaaacat tccgcttgtt tttgattggc ttcttagttt atcagttttt
    10921 attacttggt gtatcttttg tcttgccgaa tttccttcag atcgttcaag gaagtagtcc
    10981 ttttgtcgct ggattagcga tgcttcccgg cgcagcggtt ggtgctttgc tttctccttt
    11041 ttccgggaaa atgctggatc aatatggtcc gaagaagcca atttttgctg ggctgattct
    11101 ttctttgatt ggttggctag cattattcct actcatagga cacgcttcag tgggtgtatt
    11161 gattgcttgt catgtgtttt atatgatcgg tatcggatta tcttacagca atatgatgac
    11221 caccggaatg aatgcactga atgaagaact acaaggagac gggaacgcaa tgttcaatac
    11281 cttgcagcag ttttctggcg ctgtggctac ttcattagta gcggtcatta tcaattacgt
    11341 ccagcatcat acttcccata actatgaagt gtctactaca ttaggatcaa aagcagcatt
    11401 aggcgtcttg ttgctgttgc ttctcgtaag ctttgctcga ttcgcctatt atctgttctt
    11461 tgctaaaaaa gcgtagattc catatacggc aggtgttgca tctgccgttt ttttgtgtga
    11521 aaatggttgt ttgtaaaagg aggctttttt atggaaaaaa ttgccattat cggtggcggg
    11581 atcattggta tgaccctagc cagttactta gatacatcaa aattcaatgt tgctttattt
    11641 gatgaaggga caggacaggc aactaaagct agcgctggga ttatctcccc ttggttgtcg
    11701 aaacgcagaa acaaacaatg gtatcgtctg gctaaagacg gcgctgcttt tttccctaaa
    11761 cttataaaag atttccaact tgatcaaacg atttacgcac aatcagggac acttttgctt
    11821 cgacctgaaa aagatttagc tgaattggct gttcttgccg agaaacgaaa agaagaagcg
    11881 ccagaaattg gaaacatcga atggctttca cctgaacaga catctcaagc acttccgtta
    11941 ttgaaaccag cagtttcact gcgtatcagt ggtggtggtc gtctggatgg aaaagcatat
    12001 ttagaacatc ttggtcagcg tttagcacaa aaacaaatgg agcagatcca ggcaaaagtc
    12061 actttgcata agaagagcga ttcttggctc atcaagggac cgtttggaga atacacagct
    12121 gatcgtgtaa ttttgacacc tggcccggca cttaagcctt tattacaaga attaggctat
    12181 gctgcagata tacgtccgca aaaagggcag ctcttagcat ttcagacatc ttataagatg
    12241 agtcaagact ggcctgttgc tatgcttgac ggagaagctg atttcattcc tttcatggat
    12301 ggaaagatcc ttttaggtgc tacccatgaa aacgatcaag gctgggattt ggctcctact
    12361 agcgaagctt ataagggatt gactaagagt acagaacagt tcttgactga tccagatcag
    12421 ataaaaaact ggccatacga gttccgtgta ggtacccgtg cttatacgtc agatttttcc
    12481 ccatttttcg gcgtactgcc agatgatcca agcttactgg ctgctagcgg tctaggctct
    12541 tctggcttga ccacaggtcc ttatatcggc tatctattag ccaacttctt aaatactggt
    12601 cattggccta cggaagatta tcaaaaggac atctccacct atattacgaa aaatccatca
    12661 tagaaaaaca gctttttcaa gtaaggatcc cgagcgatat tgcgcatgga ttacttcttg
    12721 aaaaagctgt tattttagtt caatttctga atcaaattct ccaaactttc ttttgcttca
    12781 tctggcaaga gatcttttcc tatctgagct tcaatgaatc gtaaacgaat atcaagtcgt
    12841 tgtttcacca gatttttata ggacgtatcc tcaaaagaaa caggaaaatc tggtataggt
    12901 aaatagtgga tgtcttcaaa tggaccaaac gacttgaatt ctgccgtttg ttgcacgatt
    12961 ctttcaacga ttgacaatgt cgtttcaggt ttgatttttt tatcatcgaa atatccagta
    13021 ttcaggacat agcaggctgt ttgctgtttc aaaaacaact ctcgaaaagc ttgatagtct
    13081 tccctaagag gatagacacg gaaaggatta gcaaatggtt caatgactag ctcatctagc
    13141 tgtctttttg tagtgatatt ttccgcgtct gatcgttttg ttgctaatgt tgcaccaaaa
    13201 gacgctgcta aaactgggtc attgatcttg ataattggcg gaagcgaatc atctttcatg
    13261 atccaaaaaa cagcatcgat cttttcgcta agatgatcga ctcggtcggg tgtcgcataa
    13321 cgtgatttga ctgtccgccc atttccgttt cgtatatctc ctgttactag gactttttct
    13381 cctttacttg tacatgtcac gcccacattc tgacaagtaa ggaaagaaga aatggccgga
    13441 tcatcagacg gatagtcttg tgttttatca aagtaagaag gttccaaggc agtagcaaaa
    13501 ccattttttc gagaaataac gaaagcatcg tcatgcaaga tcgaaacaga gtgaccttcc
    13561 gtatgttttg ctaaggtaat cgttgatttt ccagaaccgg acaacccaaa tacagccatc
    13621 gtataggtct ggtcattact agcatattgt ttcatcccac cgtggcaggc aataaagcca
    13681 ttgcgatttc ccaatgccca agcaagcgtc aaagtcgctt tttttaattc tccaaagtaa
    13741 cgtaatccta gcacagccgc tgtattatgg agagcatcaa acaaaatgag tccatttgga
    13801 aatgtttcat gtgtccattc gggatcacaa aagatataaa gatctccctc atcaaaggaa
    13861 acagactttt gataagtctc taagatcttc gaattcgctg gctgaaaatt cagcaaataa
    13921 gaatacaaat tgttctcaaa tccttctgga atcagcaaat gactttgcac agaaaattct
    13981 tcagataggc caacgattac ttttgtatgg taaaactggc gcttgcgact ttgatacaca
    14041 gcttctctta aaattttctc gtatttgctt tgttccttgt ctaagtcctc aaagatccgc
    14101 cgagcacttg ctgttcttcc cacaatgctt ccatcattga atactagtgt cctaacatca
    14161 gctggtaaat ctagttcttc agcatgtgag attgttaaat ctgtgatgat cgttcctgga
    14221 ctcccttttg ctagttgata agctgtcttc ctatcaaaaa cttcatgaac attattaccg
    14281 taaaatgtcg tttctacaat cgatcgaata ctggataaca gtggatttcc acttgtgatt
    14341 tcgtttaatt cgagactctc ttttgtactc atggctcctc ctcattccct aaacaaaaaa
    14401 acgttttctt tattatacat tattcgagtt tttatttgta ctgctgcgct gtgggaagca
    14461 aacgagttat ttttcacaca aaaaaaccga gaacttgaat aaacaagttt ctcggtttca
    14521 tcattttcct tttatcggct tagtaccagc cgtttgctaa ccagaatgtt ttagcagcat
    14581 cccatgaacc ataacgagat gttacatatt gttctgctac acgttcttga ttggcagctg
    14641 aatagtcgcc gtttaagtat gaagcatcta attgataacg gccgatgtaa cgtccgttag
    14701 tagctgtgta tgaaccgcct gattcttttt gtgcgatcca ttcttttgca gagcttgaag
    14761 ttggcgctgt tgcagcagct ggggcagcag gagctacttc aactgtttca acaggtgcaa
    14821 cgtatgtatg ttcttgcgtt gctgtttcag ttgtagatgc tgtattttct gttgttttgt
    14881 tttctgttgt tgctgaagaa tcatttgaag ttgggattgt taattgttca ccaacataaa
    14941 tgcggttgat gtttgcgatc gaattttttt cagcaattgc atcgatcatt gagttgtctc
    15001 ctgcaaattt ttgtgagatc ttagataatg aatcacctga ttttactgta taaacttcat
    15061 cagcgtgagc atttgtagtt cctacaaaca ttgcagtagc accagcagca aaagttgtac
    15121 ctaaaacaat tttcttaaat gaattcatat taaactcctt tttttctccg ttttttattc
    15181 tttcgacgct attaacttta tcatttagaa atatttcata ggtaaaagaa acatgacatt
    15241 taatgacaaa ataacgaatt atttaatatt tgtaacattt aaacaaatat ttttttgata
    15301 caaaaccata aaagccttgt tgtataagca aagtaatcgt ttttctatgt ttttttattt
    15361 ttgaatgaaa aacgcagatt ctgtaacatt cactgcaata atccataaaa aaaagcaaaa
    15421 aaatttttga tttttatcaa attctcttaa aaaaatgtaa aaaatacaaa aaaaccagta
    15481 attgttacaa caattactgg acaaaaactt tgaataatgt taactatttt tttattttaa
    15541 ctttctccgc aataatttct gggcatgttc caattcgttt ataacgactt cttctgtttc
    15601 tttagctaat atttcatcta atacatcaag ccatttttcc ggttcttttg ttcctagacg
    15661 tccaatagcc caagctgctg ttcctctgat gacagggcgg acatctgtta aacattctga
    15721 aatctcaggc aatgccgttc gatcacctag attagctaaa gcaatcaatg cgtttctctg
    15781 caaaggtttc ttcccacgcc atgaaccagc taaatgccca aattgctgtt tgaagtcttt
    15841 attagacatc gaaagcattg gctttaattt tggatatatt tcgtctactt ttggttccat
    15901 ctcttcatga aaatgaaaat cttttccttg attgtaaggg cagactaact ggcagatatc
    15961 acatccataa atgacatttc gcattttttt acgatattct tccggcatca tcccttttgt
    16021 ctgagtttga taagacaaac atcgctgggc attcattctt ccgtcaccaa gcaatgcttt
    16081 tgtcggacaa gcagtcacgc agcgcataca gtcaccgcaa ccaaattctc ctggtttatc
    16141 aggctcaaat acaatattag taacgatctc accaagatag acaaatgaac cgaactcttc
    16201 agtgatcaac aatccgtttc gtccaataaa tcctaaacca gctcgctgtg ctacagcgac
    16261 atcgatcagt tctccagtat ctacttgcgg agctagacgc cattcctctt ctttttgcca
    16321 tttttctgct cttgtttgga tgaattcgat caatcggttc aaacggtctc tgagtatatc
    16381 atggtaatcg atcccccaag aagcacgagc aaactgcccg cgtttttcat ccctaggcac
    16441 ttcttcatgg atcctagtag gatacgcaag tgcaatggaa atgatcgatt tcggctgatc
    16501 aaatgtcatt tctggataaa gacgctcatc gatatttgga tgttcaaagc cagaagtatg
    16561 gccagctgct ttttgttctt ccaacgattc ttttaatgaa tcaaaaggtt ccgccgtggt
    16621 gaacccgatt ttgtcaattc ctaatcgttt gctttcttgt atgatttctt cttttaatgt
    16681 catggtcttc ctcgcttgct gttaacggct gtataaccca taatatagtt tttctttttt
    16741 cagctggaca aactggatct gctgatcggc aatcttcttt tcttccatcc atttttttct
    16801 tgcagcaaat acttcccaat aatttgttga aattaatttt cttttgttat ctaatgcatt
    16861 aaaaaaattc aatgaaactg gttttgtact taacagttca tggagcctta cctcttccca
    16921 tggcgaaaac tcagacagat cgcgggaata caatacttct tcatagagat ttttttgctg
    16981 tgtgtacgtg aatgtttgcg ggattttccc aggcctagca atttctacta gacgattata
    17041 aaacatattc attaatttgc tgttggcaat aaattgactt ttataatagg ccgactggat
    17101 ctttttgatt actccttctg ctttgatgac tgaaaataat gcaggccatg ctttgacggt
    17161 tatttcgtcg ttttgtttaa ctaatacgcc aggtgtgaag ccgactggag aaaggaacac
    17221 aaaataaccg ataccggtat tttcatctaa aggctgaaat tcatcaaaca accatttgcc
    17281 tatgtacagc tcttcatctg gttctgttgt ctcattttga tttgctatga ataatttagc
    17341 taattctgct tcaagaaatt gaaggtcttg atccttgtcg gtattttcct gctggctgat
    17401 caatcgaaag acttcttctt taatcgtata tttatcttta cgtgtaaaat tcatagccac
    17461 accttccttc cttactctaa ctataacgga atttcttcaa aaaaggtatc tgtacgacga
    17521 aaaacagcat aatttttttt catatctctt ataaaaaatg caaggtctgc tcctcaagct
    17581 agaccttgca cttttcagct ttcttttgtt tgtgacaaac cttcatggat cgtttcaata
    17641 ttccatttca tcatctgata gtaagaatct ccttgttctc cttcttttgc tatggaatca
    17701 gtgaacagtt tatcatagat tttcaaaccc gtttcttttg ctacccgttc catgcttctt
    17761 gagtctacac tggtttccac gaataaaacc ggtacttctt ttgctcttat ctgatcaatg
    17821 atcgctttca tttgatcagg cgtaccttga ctttctgtat tgatctccca tatataagct
    17881 gctggcaaat catatgctcg tgaaaaatac ttgaatgccc cttcacttgt tactaataat
    17941 tttttgttct cttcgataga agcaaaactt tccttcgctt gtgtatctaa ttcttttagt
    18001 ttttccacat atgcttctgc atttttttta tagagtgtcg cattttctgc atctttttca
    18061 gagaatatac gtgcgatttc ctccacatat tttattccgt tagataggtc tagccaagca
    18121 tggggatctg cttttgtatg ttcttcaccg ctagttaaat aaagaggttc tacatttttg
    18181 ctaactgcaa aataatcttt tccttcttct tttttagccg tttccatcaa gttatcgaac
    18241 cagctgttac ctgtttcaag attcaaaccg ttgtataaaa taacatctgc atcgcttgcc
    18301 tttttgatgt cttctggtag tacttcatat tcatgcggat ctgttccgac tggtacgata
    18361 ctgtggatat ctatttgatc tgttccgacc tcttttgcca tgtccgccaa gatcgaattg
    18421 gtagccacga ctgacaactt ttctttactt ccttcatccg cttggtcaga agcctgcttt
    18481 ccgcaaccgg ctaataccaa gaaactaact gccaagatta aaaagagtga tttttttgtt
    18541 ttcatctatc tgcttccttt ctgaaataaa attccttttg ttggagaaaa taaaaaagca
    18601 aggataaaaa atgcggtaca tgctaacacg atcgtagctc ctgatggcca attgtaacta
    18661 taactgaaaa acacaccgac aactgcactt agcataccga ttcctgcagc tgtaatgatc
    18721 attttcagta aatggttcgt tagcaagtaa gccgtggcgg ctggtgtgat caacatggca
    18781 atcaccaaga ttgttcctac tgtctgtaaa ctgactacag ccactaatgt caaaaagaac
    18841 atcaaaagat aatggatcag cgatgtgttc agcccataag cttttgccat tgtcggatcg
    18901 aatgatgtga tcttcaattc tttataaaag aaaaagacaa atatcaagac gattccacta
    18961 acgagtgctg tcagcaacaa atcgctttcc ctaacagcta agacattccc aaataaaata
    19021 tgatacaaat ctgtcgcact ctgggcatat gaaatcaaga taatccctaa tgcaaaaaag
    19081 gagctaaaga caattccaat cgctgtatca tttttcaatc ggctccgctg agtcacgaaa
    19141 ccaatcactg ctgctgttag catcccaaaa gcagtggctc caaagatgta actaaaacca
    19201 aacatataag aaatggctac acctggaaga actgcgtgag agatagcgtc tcccatcaaa
    19261 gacatccctc gtaaaataat aaaagaaccg atgactccag aagcaagtcc tacaagcatg
    19321 gaagtgacca aagcgtattg caggaattga taattcatta atccatcaat aaaggattgg
    19381 atcatttttt ctctcctcct tttactaaat gtctgatgat ctcaccatag gccaattgaa
    19441 ggttcttcga tgtaaagact tcttgtactg gaccggaagc gatcagctgt ttatttaaaa
    19501 gaataacctc atcgaaatat tcttctactt tatgaagatc atgatggacg atcaaaatcg
    19561 tttttcctga attcttcaat tcctgcaaga tgtcaaagat ctttcgttca cttaacgcat
    19621 caatccctac gaatggttca tctaaaaaga tccattctgc tccttgggct agagctcttg
    19681 caataaaaac tctctgaagc tgtccacccg atagttcgct gatctgtctt ttggcatatt
    19741 cttccatccc tacttttttc aaagcttgtc ttgcacgttc tttttctttt ttcccaggtc
    19801 tttgtccaat tcgtaaagat ggatatgttc ctaaaagtac aacgcctaat acatcaattg
    19861 gaaaggaaag atccaattca cttcgctgtt ccacgtaggc tattttttta cggactgagt
    19921 ttaccgactg tccagaaaag gtgactttac cagtagtagc tggtatcaac tctagcaaag
    19981 atttcataaa tgttgatttt cctgcaccgt ttggtccgat aataccggta attttttgtg
    20041 gatttatcgt caagttgata gttttcaaaa cagttttccc ttgataagcg gctgaaaggt
    20101 cttgaattac aatggccgta cgttccaaat cttacactcc tttttaggtt tacctaactt
    20161 tttatttaaa tatagtttaa ctaaaatgga aagcattttc aagtagaatg atgaaaaagt
    20221 ttttttcgaa ttttcctcgc attttatgaa aattttctgt agaatcacat caagagttca
    20281 ataaggctaa cctaataatg aaaaacatgt gttgaaacgt ttgattattt tttcttgttg
    20341 actcaacaac gaaaggagtt tttgtatgga agaagaaaag ttaactcgaa gtcttagtgc
    20401 ccgtcatatc caaatgatcg cactaggtgg aacgatcggt gtcggtctgt ttatgggtgc
    20461 ctcctcaacc atacgttgga cagggccctc agtgatggga gcctatgcga ttgcaggatt
    20521 ctttttgtat ttgattatgc gtgctttagg cgaaatgctt tacgttgatc cttcgactgg
    20581 ttcttttgca aattacgcga gcgagtacat ccatcctgtg gctggctact tgaccgcttg
    20641 gagtaatatt tttcaatata ttgtcgtagg aataagtgaa gtgattgctg taggatctta
    20701 tatgaattat tggtggcctg atttgccggc aattattccc ggaatcattg tcgtcttgtt
    20761 tcttatgctg gcaaatctta tttctgtcaa ggcgtttggc gaattagaat tttggttttc
    20821 gatcatcaaa gtcataacga tcattttgat gatcattgca ggtcttgggg taatcttatt
    20881 tggatttggt aatcatggtc aggcagtagg gatttcaaat ctttggaaaa atggtggatt
    20941 cttcactgga ggcgtcaaag gatttttctt tgcattatcg atcgttgttg cttcctatca
    21001 aggaatcgaa ctgatcggaa tgactgctgg tgaagcagag aatcctaaaa agacgatcat
    21061 tgaagctgtc cagtccacta tcggtcgaat cttgattttt tatattggtg ctatttttat
    21121 cattgtgagt atctatccgt ggaatcaatt gagtgagatt ggttcacctt ttgtacaaac
    21181 tttttctaaa gtcggcatta catttgctgc aggactgatt aattttgtcg tgattacagc
    21241 ggcattatca ggttgcaatt cagggatttt cagcgcaagt cgtatggtct atacacttgc
    21301 cataaatgga aagatgtcta aaaaatttct gaaactatct cgtcacggtg cgccttttta
    21361 tcctgtcgta gcaatctcag gtggaatctt aattggtctg atcttaaatt atcttctacc
    21421 aatgttcatc aaccagtcag aaaatatttt cgttttcgta tacagctcaa gtattttgcc
    21481 tggtatggtt ccttgggtag tgattttgat cagcgagatt cgcttcaaaa agaagcacgc
    21541 agataaaatg catgatcatc cttttaaaat gccactatct ccttatacga attatgtgac
    21601 acttgctttt ttagctatcg tattgatttt catgtttatc aatcctgaaa cgagaatctc
    21661 attgattatt ggactcgttt tcttgcttta catgacgatc cattactttg tacgcgaaaa
    21721 aagaaccgga aaataaaaaa agaacgaagc ttcaccttag tggaagcttc gttctttttt
    21781 attgctttta tcaattcaat tttagcgtct caccgggagt caggaaataa ttctccatgc
    21841 tcatcccatt cagcgacaag atcgtttcta ctggtacgcc agttcgttcg gctatttgat
    21901 ttggaccttc tccttccaaa actacggttg tcgcagcctg tgaagtcgcg ctgttggtat
    21961 tagtttcaga ggaatctgtt gtgtcttgtg ttgttcgagc ttgcatttca gaagcttcta
    22021 ccgactctgc tgtctcagaa tcagaagatg ttgtgtcttg tgtgcttgtt gactctgctt
    22081 gaagagaggc gctagtctgc aaataaatat cggaaatctt agcgacatga ttcgtataat
    22141 tagccgataa ttgattgatc aaagtatcta agtctacacc gtctttattt tctgctgcct
    22201 gatacgctgc acgtaatttt ctgatccctt cgtcggagat tgcttgaagc tcacgtgctt
    22261 tttggttagc aactgctgat aaaccagcta ttccctcttg atttccctga atttctgtct
    22321 ggtattcttc aatcaatgca ggcgtctttt cttcgatctt tttcgaataa tcatttacga
    22381 ttgcttgaag ttgctctggc aaaccatttg ctgttttttc ttcagaagtt tgtgtctctt
    22441 ttgttttagt tgatgactca cttgtttgtt tcgtctctgt tttctttgtc gttgtttccg
    22501 ttatggtcgt acttttcgct tctgacgaat tttttccatg aaaatataga cccgcaactc
    22561 ctcctgctat gatcaaaaac acggcaactg ctgccccaat catgatcatc cgcttacgag
    22621 ctgcctgttt ttgctgtctt cttcgggtca tccgaacatt ttctccccac tcattttcat
    22681 catacatttt tttcaccacc aatttttatg tataatagcg ttatcaaacg cttcatattc
    22741 ttattgtagc aaataattgt cgctgtgcac atttttatct ttgttattta taagaaaatt
    22801 tcaccagttg agatgattcc aagattgtcc gtcgatttct ccttgcagct gcattgttcg
    22861 ttcgtctatc tcagccatca gtttctttat ttcatataag agcgcacggt cttgatattc
    22921 tgatttttcg cttaattcat ctaattgttg gatcagccaa tcatttgtca taatgaagtt
    22981 cctttcattt cttgcagtgt aaaggtcaag tcttcgatct gctcttttgc ctcagtcagt
    23041 gcttctacga tatatgcttg tttttcagat tcttcttcct gcattttctg attttcctgt
    23101 actctttgaa taaaccagtc aatccgctga gtcagcgatg gtttgccttc caatgagaga
    23161 tactgctgat aagcaattag cttctcccaa cgtttttcct gggataaata cataaattgt
    23221 gctaaaacga atggcggaag ataggtcata tggaatttat ttgcgattcg ctgatacggc
    23281 ctcaaaaatt cggaaatcgt atattcttct tttcctccag cttggtattc tttttccgcc
    23341 acacctgttg tcaatacgat tcctagttct ttccctttta atacatgtgc tgctcgttcc
    23401 aagacattat cttgccaaat ttttaaatga gcaggagaac tataccagta aaacggaaac
    23461 tggaaaatga tccgatcatg ctgggccaag agctgtagtt cagtctctat atctattttt
    23521 ccgtctggat aacttgattc caaatgatgc caagtagctg gtaaaccagc agaagcttct
    23581 ttgaaaaatt gctgatttaa agattcttcg atcgttggat ggctaatgat gataagtgta
    23641 cgcatccctt ttcctccttt ttagtcttcg ctttacagtg tagcataacc gccacaagtt
    23701 tcttttgccg atgcttgtca ttttaaccag actaatcagg ttgtccaaga aactgcggtt
    23761 tgtttttcca actgtttttt caaggtatcc aaagcaatat tcttaaaatt ttcgtttttc
    23821 atacgtttac gatagttcgt agccgtttct ccggtactct ttttgaacat tcggctaaat
    23881 acgactagac tattgaaccc tacttgatag gcaatacttt ccacggattc atccgtcaga
    23941 gaaagaagaa acgcccctcg attgatccgt accccgatca aatattcctt gatactggta
    24001 cccatatgat ttttgaatac tcgcgacaaa tggctccgat tgaccgctaa atgctgggcg
    24061 atttcctcaa cagacaattt ttcagcataa tgtgcctcga tgtatttgac tgtttcaaca
    24121 gctaaagagg aatgaacctt ttgtacacca ccgctgacct ttccgccatc ttttaaaagg
    24181 actgataaaa aacggtaggt taatgctgtt aactgtaatt cattttccaa ggtttcttcc
    24241 gtatctttca tgcattctaa aataatgtcg atatattttt gtatgtcaga agagaccatc
    24301 gtgtattggt cttctctgaa aagcgactgc cgaatgattt cttctgcagc actcccacca
    24361 aatgaaagcc agcaatacat ccacggatct tctccatctg ataagtaaaa cgtcgagtct
    24421 cctggacgaa tcaagaacag atcacctttt ttcaattgga attgttgatt tttcacatga
    24481 taagatcctt tgccttccat cacgacatgc aggatgtaat cattgcgaat agcaggacca
    24541 aaagaatgaa acggcatcgt tttagagaaa ccacaaaaat caaaatacaa ttctttttct
    24601 acttgttgat gttccttttt taaaaaaatc gattgctcca cgtctatccc tccttactcc
    24661 tttagtataa tgcaacaaaa agttaaataa aagtcacaga aatatattaa aaaggaagcg
    24721 atttcttgtt aagtttaagt cattaaaaga tctttgttat tcgttgtaac cggtttactt
    24781 agtattatat attgaacagg agaggaatac agattatgaa aagcaaaatg atccaacgtg
    24841 tgtcctacgc tttaggcgct tttggacacg acacattcta tgttacatta agcacttatt
    24901 ttatggtatt cgtgacaagc cagatgttcg caggaacaga taaggcaacg aatgcaaaaa
    24961 tgattgctac agttacaagc ttagttgtag gtatccgctt agttgaaatt atttttgatc
    25021 ctttgattgg tggcatcatc gataatactc gtacaaaata cggtaaattc aaaccatggt
    25081 tagttatcgg cggcgttgtt agttccgtac tcttggtagc aatctttacc aatttctttg
    25141 gcttagctac tcataatacg actttattta tcatattatt tgcaatcgtt ttcatcttat
    25201 tggatagttt ttattcattt aaggatatcg ctttttggtc aatgattcca gctttgagta
    25261 cggaaacaaa ggaacgtgaa aaatttgcta cgtttgctcg tttcggctct tctctaggtg
    25321 caaatggtac tactctagtc gttgtcccta tcgttacttt ctttacctat cttgttactc
    25381 ataaacatat tgaaggcgct tcaggctggt tctggttcgg tgtgattgct gctgttattt
    25441 caggcggtac tgcgattatc actgccttgg gcacaaaaga agtcgatact gcgatccgtc
    25501 agcaaaatga aaaagctggg atcaaagatg tgttcaaagc agttgctcaa aatgatcagt
    25561 tgatgtggct tgcccttgct tatatcgctt atgcgattgc caatgtagca acaactgccg
    25621 tattattcta cttcttcaaa tatgtacttg gccaaccaac tgaattttgg atcgttggtc
    25681 tagttgctgc cttacttggt ttagtagctg ttccactatt cccaatgctt acacgcgtca
    25741 tctctcgtcg ctacgtttac cttggaggga tcgcaatgat gatttcttct tactttttct
    25801 ttacgatcgc aggttcaagt ttgccaatcg ttatcatcgg gctagtattg ttctacttcc
    25861 ctcaacaatt gattttctta tccgctttga tgacgatcac agactctgtt gaatatggtc
    25921 aatggaaaaa tggtatccgt aacgaagctg ttactctatc aattcgccct ttactagata
    25981 aaatcgctgg tgctttgtct aacgggatcg tagggtttgt cgctatcgct tctgggatga
    26041 ctggtaatgc tactgctgct gatatcactg ctcatggcgt aacgactttc aaagcttatg
    26101 cattctatgc tcctgctgct ttgatgattt tctccgctat tatttttgct tggaagatta
    26161 cattgacaga gaaaaaacat gcacaaatcg ttgagaaatt ggaaaacaaa ttggctccag
    26221 ctgatacaat ggaagatgaa cttcacgatg cagtagttcc ttcccattaa gaaaggatct
    26281 tgaaaatgaa gattacagaa aaagattttg gccaaggtta tcacttgata accttggaga
    26341 acaaaaacaa gctagcactt tctatctctg atttaggtgc tagaatcgtc agtttaaaat
    26401 caaatgatcg agaattagtt ttgggatttg acaccgctga agaatatatc gaaaaagatc
    26461 cgtatatcgg tgcttctatc ggtcgcaccg cagggagaat cgaaaatggc cgcttttctc
    26521 tcaatggaaa aacttaccaa ttagccacag atccaaaaac tggacataat ctgcatggtg
    26581 gtgctcctgg atttgaatta aaaaaatggt cttatgtcat tttgaatggc gaaaatgaag
    26641 cttctgtcat ttttactact acaagtcccg acggagaaca tggttttcca ggtacaatgg
    26701 atgtggaaat ccgctatact ttgacaaaag ataatatctg gcgagtgacc tctcgcggca
    26761 ccagtgacca agatacttta ttcaatccga caaatcatgt ttacttcaac ttgacgggag
    26821 atgcttctca gtcaatcgat caacatgagc tttggctcaa tagcgaagct tatgctcctt
    26881 tgcgaacaga tagtattcct ataggggtaa aagaaaatgc tgcaggaagt gctttcgatt
    26941 ttcaaatacc taaaaaatta gcgtccgttt ttgctagtga tttggatcaa aaaaatcttg
    27001 ttgacggaat cgatcatcca tttttcttaa aagaaaccgg cttgggtaaa gaagccgctc
    27061 gcttgactag ccccgataaa agaatccaag tagatattgc tacagatgct tctagtgtgg
    27121 ttattttcac tgcaaatttc ggcacagaaa caccagaaat gcgcaaccga aaactggcac
    27181 atcacggtgg catcacattt gagactcaaa cagctccagg tgcagagcgc ttctcagcat
    27241 ttggttcgat tcatttaaaa gccggatcag tatttgaaac agtaacagaa ttcaaaatca
    27301 aaacaagaaa ggaataactc atcatggaaa tcaaaagaag cttaaacaca aaatttgccg
    27361 aaacattcgg acctaaaaaa actacacaat acttctcacc aggacgcatc aatttgattg
    27421 gtgaacacac agattacaat ggaggccatg tcttccccgc ttccatcact tatggaacgc
    27481 aaggtgtagc agcacctcga gacgataata aaatattagt ttattccaca aactttgacg
    27541 atatcggtgt cattagtttt actttagatg agctagaata tgataaaaaa gcaaactggg
    27601 caaattacgt aaaaggaatg gtccttaaat taaaagaagc tggacactca atcgatcatg
    27661 ggtttgagtt attagtggaa ggaacaatcc ctaatggtgc aggactttcc agtagcgctt
    27721 cattagaatt attagtaggt gttgtattag aagatctgtt tgatttaaaa acagatcgct
    27781 tggaactagt ccaaacaggt aaaaaagtag aaaatgaatt catcggtgtc aattcaggta
    27841 tcatggatca gtttgccatc ggcttcggtg agctagatca tgcaatctta ttagatacaa
    27901 atacgctgaa atacgaaatg gttccagtga aactagatgg ttatgcaatc gtcatcatga
    27961 atacgaataa gcggcgggaa ttggcagatt caaaatacaa tgaacgccgt agcgaatgcg
    28021 aagaagcctt ggcacgtttg caaaaaactt tgcctatctc ttctttagga gacttagatg
    28081 aagaagaatt tgaatcaact attgaccaaa tcggcgatga tacattgata cgcagagctc
    28141 gccatgccgt ttctgaaaat cagcggactt tgaaggcaaa agctgaatta gaagctggga
    28201 acttagaagc atttggtcaa ctgttaaatg attctcacca ctctcttcgc tatgattatg
    28261 aagtaactgg gatcgaatta gatacactag tagatgctgc tcaaaaacaa gaaggtgtct
    28321 taggtgcacg aatgacagga gctggatttg gtggctgtgc gattgctctt gtcaaagaag
    28381 aaaatatacc tgccttcaaa aacaatgtct atgatgaata catgaaagtt gtcggctatg
    28441 ctccggaatt ttatgttgca cacattggaa atgggacaac aaagatcgat ccagaagctg
    28501 ttcatgtttg ataaaaaaat caacgatctc agctaaaaac aaatagataa aaataacctc
    28561 tcagcaattt ccaaatgtga gccttttgct cgtatttgaa aattgctgtt ttttattgac
    28621 acggaaagag aacgtgtgtt ctaattgtgt tgaagaggtg attgttatgt gggcgaatca
    28681 aaatccggta tttgtttatg aaaaagaacc ttcaagagat attttatgta ttgactgcaa
    28741 atctttttac gcctccgtag agtgcgttga aaaggatctt gatcccctta caactaaatt
    28801 agtagtcatg tcctatccgt ccgattcgac agaaacacgg ggtagcggat taatcttggc
    28861 ttccagtcct gcagcaaaaa aagcttacgg catcaccaat atcagccgtg ccagagatct
    28921 tcccttcccc tatccaagtg atttatatat tgttgcccca aggatggcct attatatgga
    28981 aaagaacaga gaaatcaatt ctatctataa aaaatatgca gacgaagcga accatcatgt
    29041 ctattccgtc gatgaaagct tccttgatgt aacaaatagc atggcactct ttggtaaaac
    29101 aaatgcgtac gacatggcta aactgatcca taaagatgtg tttgagcaga ccggaatcta
    29161 cacgactata ggaattggcg ataacccgct acttgctaaa ctagccttag acaatggctc
    29221 aaaagacgga aagcaaatgt ttgctgaatg gcgttatgaa gatgtaccag aaaagttatg
    29281 gaaaatctca gatatcactg acttttgggg aatcggcaaa cgaacagcaa aacgtctgaa
    29341 acaaatgggt atacattctg tttatgattt agctcacgcg gattattatc acttgaaaag
    29401 caagctaggg gtcatcggta ctcagcttta tgcccatgct tggggcatcg atcgaagttt
    29461 tttgggacaa gaatatacac caaaatcaaa aagtatcgga aatagtcaaa tcctagatag
    29521 agattataca agacgagaag aaatcgagat cgttatcaaa gaaatggccg accaagtcgg
    29581 cactcgcttg agacgatctg cagcaaaaac acaagtagtc agcttatggg taggatactc
    29641 tttaggctat cttgatccaa atggcaagac cgggtttcac cagcaaatga aaatcgaagc
    29701 gacaaatgct agcagagatc tagcagatgc tttattgacc attttccacc agcattacca
    29761 ctaccaggat attcggcgtg taggcgtgaa ctgttccgac ctcgtatacg cgacaggttt
    29821 acagcttaac ctttttgaag atcctgaaaa gcagataaat gaagcgaaag ttgattttgt
    29881 tgtcgatacg attagaaaaa aatacggctt tcaatcaatc atccatgcgc atagtctctt
    29941 gcaaggagga cgcgccatcg cacgtagttc tctcgttggc gggcatgcag gtggtatgag
    30001 tggattagaa ggtggaaaag atgagacgga cagcaaaaaa attcattgac tataacgagt
    30061 atcgagatcg gccatttgga ttaaaatggg ggactgcttt tgcgatggaa gaactagtca
    30121 acggtatcaa aaaaatgaag aagaagcacg caaacagcct cttcctaaac aacaaatgac
    30181 attgcaagaa atcgatagta tactgacaga agccttttta tctcgtaaag cagtaatcat
    30241 tcagctcaat ttaaaagatg aatttggaag actcttggat gaggtcgaag ggcacttcat
    30301 tggtgaagcc tatgaagatt actttatgat tgaccatcag ccgatattgt gggaagacgt
    30361 acggtatatt gagttaaagg aaacaaaaaa atggtttgtg acatgagagg ttgtgacaga
    30421 agtgatcaac ttcaaaaaat aagaaaggat ttttcgaaaa ttgcttctta aatttttgtg
    30481 gaattttggc ttatttttcg aagaagcaac ttctggaaca acgtatatta gatatcagag
    30541 gttgtgaaaa agtcgcttag ttttgagtaa caagccaagg cgctgatttc acaacctctg
    30601 tctatctatc catctattcg gtaagattca tgcatgacgt cttctttatt tagtatgtag
    30661 agcccttttt cttctgacca ttcaacataa atcagctgtt ttacatctgc atagccttct
    30721 ttcttcaagt tctccataag ccatgcttca gtcaattgat tggactgaag cactttatgc
    30781 tgaatttgcc cttcatctac aagcaggatc gataaagctt tgtcttcatc tgattttttc
    30841 aatacactca tttgaccggc attctctagg ataacatgtt tggcattttc aagaaaaaag
    30901 cattcttgct gtcttaagat tgctcgcaac tgctccatct cgatatggtt atttgtcaac
    30961 tctgtcatgt tgatgacgcc gtctttgatt agaacagatg gctcgccttt gacccaacga
    31021 ttgacctttt catttttttg gacgatacgt tcgataccat aaatcattac tgcccaaagc
    31081 gcaatggcaa ataggacatg ccctacatgg acattgtcat cataggttga ttcttctaag
    31141 atcccaccta aaacaagcgt atagactaaa tcaaacggtg tgatttctga catggatttc
    31201 ttcccaagca aacgtaccac taataataaa ccgatcaacc cgaacaccag tttcattgta
    31261 attgaaaaaa tcattttttc tccccctaac tatctgttaa ttaaattata tatgctttct
    31321 cacgaacacg catggaatat acaaggaaga aatgtatcct tttactaaaa aaaggactgt
    31381 gacactgtag tgacccccaa aagttagact tttttggagt ggagaaatcc actccttttt
    31441 tttatgctct aaaatgatat ttttacacaa ccgttacggt tgttttacga aattggacg
//
LOCUS       DACDIE010000030        30556 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.030, whole genome shotgun sequence.
ACCESSION   DACDIE010000030 DACDIE010000000
VERSION     DACDIE010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30556
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.030"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(451..1761)
                     /locus_tag="IV250_001499"
     CDS             complement(451..1761)
                     /locus_tag="IV250_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP8768789.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1773..3191)
                     /locus_tag="IV250_001500"
     CDS             complement(1773..3191)
                     /locus_tag="IV250_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8768790.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3431..4999)
                     /locus_tag="IV250_001501"
     CDS             complement(3431..4999)
                     /locus_tag="IV250_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAP8768791.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(5003..5413)
                     /locus_tag="IV250_001502"
     CDS             complement(5003..5413)
                     /locus_tag="IV250_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAP8768792.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5410..6333)
                     /locus_tag="IV250_001503"
     CDS             complement(5410..6333)
                     /locus_tag="IV250_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HAP8768793.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6330..7208)
                     /locus_tag="IV250_001504"
     CDS             complement(6330..7208)
                     /locus_tag="IV250_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP8768794.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(7195..8532)
                     /locus_tag="IV250_001505"
     CDS             complement(7195..8532)
                     /locus_tag="IV250_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP8768795.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8535..9638)
                     /locus_tag="IV250_001506"
     CDS             complement(8535..9638)
                     /locus_tag="IV250_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP8768796.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9712..11220)
                     /locus_tag="IV250_001507"
     CDS             complement(9712..11220)
                     /locus_tag="IV250_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP8768797.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(11186..11878)
                     /locus_tag="IV250_001508"
     CDS             complement(11186..11878)
                     /locus_tag="IV250_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP8768798.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11900..12874)
                     /locus_tag="IV250_001509"
     CDS             complement(11900..12874)
                     /locus_tag="IV250_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8768799.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            13174..14046
                     /locus_tag="IV250_001510"
     CDS             13174..14046
                     /locus_tag="IV250_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8768800.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14504..16702)
                     /locus_tag="IV250_001511"
     CDS             complement(14504..16702)
                     /locus_tag="IV250_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8768801.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16934..17926
                     /locus_tag="IV250_001512"
     CDS             16934..17926
                     /locus_tag="IV250_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8768802.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17986..19407
                     /locus_tag="IV250_001513"
     CDS             17986..19407
                     /locus_tag="IV250_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAP8768803.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19400..20899
                     /locus_tag="IV250_001514"
     CDS             19400..20899
                     /locus_tag="IV250_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAP8768804.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20938..22332
                     /gene="uxaC"
                     /locus_tag="IV250_001515"
     CDS             20938..22332
                     /gene="uxaC"
                     /locus_tag="IV250_001515"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAP8768805.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22491..23174
                     /locus_tag="IV250_001516"
     CDS             22491..23174
                     /locus_tag="IV250_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HAP8768806.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            23176..24012
                     /locus_tag="IV250_001517"
     CDS             23176..24012
                     /locus_tag="IV250_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP8768807.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            24023..25777
                     /locus_tag="IV250_001518"
     CDS             24023..25777
                     /locus_tag="IV250_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8768808.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(26053..26394)
                     /locus_tag="IV250_001519"
     CDS             complement(26053..26394)
                     /locus_tag="IV250_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP8768809.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26462..28633)
                     /locus_tag="IV250_001520"
     CDS             complement(26462..28633)
                     /locus_tag="IV250_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP8768810.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            28844..29701
                     /locus_tag="IV250_001521"
     CDS             28844..29701
                     /locus_tag="IV250_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP8768811.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(29772..>30556)
                     /locus_tag="IV250_001522"
     CDS             complement(29772..>30556)
                     /locus_tag="IV250_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8768812.1"
                     /translation="LSIPTTTPEETLDQVFEFFDRYELKGIGIGSFGPIGIDKTKDNY
                     GYVLATPKKGWAQFDFLGTIKERYNIPAAWTTDVNAAAYGELKQGAAQGKNSCIYLTV
                     GTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPDDHYEGTCPYHKDCLEGLAAGPSIEA
                     RTGIKGQDLPQDHPVWAIQAYYIAQALINYTLTLAPEKIILGGGVMNQDHLLQKIRQQ
                     FVELMGGYMETPQVSEYIVRWGLPNESGIIGSLLLAEHEHQK"
BASE COUNT         9123 a         6290 c         5401 g         9742 t
ORIGIN      
        1 ttgtcttgaa ttgaatcaag acgccctaca catttggttt gtcttacttt gttcagtttt
       61 caaaggtcta cttcatcgag agtgtttccc gattcatttt tggctgccgt agcaactttt
      121 atatcatatc agagagacaa acgattgtca actctttttt aaaagttttt ttcgatttcg
      181 tttttcgaaa tctttcagca actcatttat cataacttat cgtttgttat ttgtcaagaa
      241 cttttttgaa gtttttttcg atagaatcaa gcttcgcaag tggttctttt taacgactta
      301 gttatcttat cacgtctaaa aacatgtgtc aactcttttt tggaattttc ataaaatgtt
      361 tcaatgaagc gcgacaacgt tgtttaatat atcattaaaa aaaataaagg tcaagctaaa
      421 atattattta agcttgacct tattataatt tcaacgattg tcaaaagtat tgtttgcagc
      481 tgtgttgcca gtttctatag ataaatcaca gcttttgaaa cataagtttt gtactgcttc
      541 gattcgcccg aattcttttg cagttatttg gcaatgagaa aagcagacat cttctatagg
      601 cttttcagga aatgctacta aatcaaaagc tcgggaagga agtgagtaac ctggagaaag
      661 gcatgcctgt acgtttttga tgtgtatgtc tttgactttc gtcatttgct ttgattcagg
      721 tatttcttca gccactgctt gccaactttc tggcatttga gcaaggtcat tgctattttt
      781 tctattgtat tcattatgcc agtccattat ccaagaaaaa ggaaattgta catcaatcat
      841 attcagattt tctaccctga tattttcgat cactccccct ctttcttttg aagatttcat
      901 tcgaatacca cagtctgact gaaaaaaatc gatatcgtga atatatacgt cactaactcc
      961 agcactaact tcgctaccaa tagttacacc gtatcctgaa cggattttac atctagcaac
     1021 ctcgattctt gatgattttc gattgactct tgcaccattg ccatctctgc cagacttaat
     1081 agcaatacaa tcatcaccac aggccaaatc acattcatag acacgcacat ttgttgaaga
     1141 atcaatatca atcccatctg tacttggtcc atcattgttg cggatgatta ctttttctac
     1201 taatacatcg ttagaataag tgatttgcaa gttccaaaaa cctgatttct gaaaaatcaa
     1261 atcacgaaca acaacatgtt ctgcatgata cagtaaacaa gcgcgtggac gtttaatagc
     1321 ataatccgca atccaacgaa gtcccttttg atcgtattca gctcttgtcc cgcttttttg
     1381 atcttttccc caatacagct cccaccaatg gtctccttga ccgtcgatta ttccttttcc
     1441 ttctataaga atatctttcg ccgagagaac attcaaaatc gctgctggcc atttcatttc
     1501 tacgccggct actcgtgtat cgatttctgg aaatgcttcg atatctgttc tccccttgag
     1561 gactgcacct tcttcaaaga taagatgact cttatctttt aaaaaaaggg ctccgactaa
     1621 ataaatgcct tttggaataa atacaggtaa gtgttctttc gcaccttgat cgattgcttt
     1681 ttgcagtatt attgtatcat cttgttgagt gtcgtttgtt ttaaagttca aagcattgat
     1741 aatccctttt tttgtccgca atacgtttct ccctatcttt cattgatttt tttcaattct
     1801 tcaccaataa atacagtttt tgaacgattc agtgggtaac ctactagcaa aatgattgcg
     1861 gatacaaata agcagaatgc tggtaacaag tttgccatcg tataaatcct ttcttgtact
     1921 aaaactgatt ggatggctcc acctgttgta gacgattggt aaccaatgag tgccagcata
     1981 aatccgccta ccgctcctgc acatgcttgt cctacttttc tcgcaaaaga atttactcca
     2041 tatactgttc catcttctct taagcctgtc ttatattgat gataatcaat cacgtcagta
     2101 atgaatgccc agaccatcaa attgaataaa ccagatccta aagtagcgat aaataaaaga
     2161 atcagatata cccatgcatt tgttatatga aggaaataca tcagcaagta catacatgaa
     2221 gaagcaagta acgcaaatac gcttgcttcc tttttaccaa ataattttgt cagctttgta
     2281 gcaaatggtg ctaaaactac tactgttccg taagtaaata aaagagcaac agacattgct
     2341 tgtttattat ggaagtaatc attgaataaa taagtcatat tcgtcccagc taaaatctga
     2401 ttgatcacaa tgaaaatgtc aacaatgact aaaataatca gtgctcgatt agctaacaaa
     2461 ccttttacca actgagaagc agatactttt tcgtttttga caactcttac tctttcagta
     2521 gtcaatttac aagttaacat atatgcgaca aatgctattg cagcacatcc gattgaaatc
     2581 caaaagaaat gattccctga aacgacttgt tgtccactct tgttatgggt atacattaaa
     2641 atcggaatga agaatcctgt agttgatccg ccaatcgcag aaccaatgct tcggaacgtg
     2701 gacaacgagg tgcggtcatt aggatttgag ctaattgctg ctgccattgc tccatacggg
     2761 atattgattg ttgagagaaa aatcccataa acaatgtatg aaacaaaaat ataagcaatc
     2821 ttcaccgtca ttggaagatg ttggacgaat ggcaaaaaca aaatgacagt cactaagcaa
     2881 aatggatact tcatttttct gatccaagga ataaatcggc cagtttctgt taactctgag
     2941 acatcacata atcttccaac catcacatca gcaaacgcat ccaaaattct cgaaatcaag
     3001 aataacgtac ctacgattgc ccctgaaacg ccaagaacat tcgtataata aatcatcaag
     3061 aaactattga ctaaacctaa aataaaacag ttacctagat caccgaacat gtagcctatc
     3121 ttatcggaaa tcccaaattc ctttttttct ttttttattg tatttgctgt aacagcattt
     3181 tgtgaaagca tttgaattcc tcctacctct tctagtaaac gttttcaaaa ttataactct
     3241 ttttttcttg tttttatatt cacaatgttg gctttttatt gcattatctt gacagataaa
     3301 aaacaaaaaa taatctaaaa acattgatgc ataagattga taactttttt tacataacaa
     3361 tatatacaaa caaaaaaatt cactaactag tgaaaataag ttagtgaacc tcatttatta
     3421 aagaattaaa ttacagatga tcttttccat catacttgaa aaactcaaaa tcagcataac
     3481 ttcctctttt tttatttaga tcatgcactg ctatcccgat aaaatttcca gtaaaccctc
     3541 ctgagagtat acacatgttt ttttgcggac ctactttctt ccattcttca gttgatacgt
     3601 ctttcaaatg aaactgtgct gtctctttct ttacagtgac tttcaaaaaa gtcttctttt
     3661 ctgtgagtgg aatcgtccaa tcttctattt gtgctactcc gttttctgat tctatcatac
     3721 gtaggcattt tcccttgtcc tcattccaag taacataaca ataaatataa ttggattcat
     3781 ttaagtacag taccagtcca gccatctgat taaaattatt aggcatgtat tcaaatgctg
     3841 tctccgcatc aaaagtaaaa tccgtttgcc gaatagctaa caaatgatgt tcgaaggtag
     3901 attgaagaga ttcaccggaa atcagacgta aaaaacctgg tctacatttt gtatcacacc
     3961 aatcagcatt tggcaaaatg cgtctggcat tccagttctt ctttagctct ccttcaaatg
     4021 tatccataaa ctgatgatct ttttcttgtt gtacctcttc atttgtctca atcattattt
     4081 cttccatcgg agttgtctgg ctatctttaa gtcttaacca atcgtcggac gtccaataca
     4141 cttcttgaat agcggtttct cttcccaaaa tagccgcacc gtttaatggt ctggtacaaa
     4201 gatatgtcat gtaccatctt cctgacgggg tttgtactaa gctcccatgt cctgtacatt
     4261 gcaatgtaga agcaggtttg tcactagctg tcagcatagg aaaaccagga tcaagctcgt
     4321 aaggaccagt aatattcttt gctcggcaaa ctgtaactga atgtcctctt cctgtaccgc
     4381 cttctgccgt catcaaataa taatacccgt tacgaaaata caaatgtggt gcctccgttt
     4441 tcgctaattc tgtacctgca aatattttat agatgggacc atcaagcttc ttcttctctg
     4501 catcatattc ttgtaatacg ataccgctgg atttattcgg tgtgtccaga cgataatccc
     4561 atagttcatt gacaagccat ttttttcccg tttcaagatc atggaacaaa gaaggatcaa
     4621 agccgttact atttaaataa attggttcgg accaaggacc tgtgatctca gatgcagtga
     4681 tcaaataatt gtggacatct ttaaacggac ggcctgcact tttgacatct gtgtaaacaa
     4741 gataaaacaa tccatcagca taactgatct gtggcgccca tatacttcca tttgtaggat
     4801 ttccttgcaa agatacttga gtggtcaaaa tatccgttgt atgtgaccag ttgactaaat
     4861 ttttcgactc atatactcgg attcctggaa gccattcaaa agaggagaca ataatgtaat
     4921 accgctcttt cactcttaat atattaggat ctggattgaa tcctcttaaa ataggatttt
     4981 tcactttgat agtctgcatg attcaggctc cttttctcat tcttactcga tataaactga
     5041 atgccccagc tgcgaaataa aaaaatgcaa aaggaaacgc aataaactga gtgccaatca
     5101 acaatatgaa tcctgttata aaatacaaac cagtggcagt tgttaagtat tttcctttag
     5161 cagtcaaaaa caatgctact gctaaaaaag caagtgaaat aaacaacaca acggcaaaca
     5221 gaccagtcat gtcctgtaat aatccaaatg tattttctaa agacaaagtt gaatgatcta
     5281 cagcaagcga ctgataactt tttttaaact ctgacgcaga catattactt aaagtaactg
     5341 ctaacccgcc aaagaaaatc aaagatagac tgccaccgac cattcctagc aatagttccc
     5401 attttctttt catttctgaa ccccctgctt ttctaaatgt atccattcag gtcttttgat
     5461 cgatgtttca taattgttat tacgttcaga gaatttgaca gtctgttcat ttttcttgtc
     5521 atcccaatta tcccaaccag ttgggtgaac atgcccacct atcttacacg cttcaaataa
     5581 gacatttgca tacggacgcc acggtcttcc taaatagacg tttctagtat caggttctgc
     5641 agtcaaggta cagctttcaa aacgataccc tgttttttga tttttcggcg tagatgctgc
     5701 tgtgatgtat ccaggatttt tactggctaa gctatggata tgacactgat gaaaaacggc
     5761 atctgcccca ccaaatatga aatccaccgt tccttcaatc cagcaatatt cataggtctg
     5821 acgacaatac tcaggattgg cagaagccgg tgtacaaaaa ggagtaccat ctttttgcaa
     5881 atcaggcaac ggacccgtgc ataaggtatc ctgataacca gatagtcgac agttttttaa
     5941 agatacgcga tctcctgaat tgaataaagc aacagcctgt cctacctttt ctcctggacc
     6001 tgctgtattt tcgatgtgaa gattttctaa tacgacattc gatccttcta agaaaaaagt
     6061 agcacttcgg aaagtcccta actggctacc gtcaagatac tcctgttttg caaaacgatt
     6121 accggagata actactgttc ctgctcccac aagtttaaaa ttggataatc tgctttctat
     6181 atattcatca tatcttcctt cttttactat caagaccttt ggatggctat ccggcaggcc
     6241 tgccaaataa tcgattcctt cttggatagt ttcaaaatcc gtcggggtat ctatagcatt
     6301 ctttccaatt atcagtttgt ctttcatcct cataaatgct cccttatgaa ttgtttccaa
     6361 atatttctca tttcttgtgt ttccatatga ccgcctgtta aagaaaaaga agaaaagttt
     6421 tctggactac ccattgattg atactttttg tccagccctt ttctcagctt cagtacgcct
     6481 tcgtccaagc acatcgtatc gttttttcca actaagctaa gacgagggcg cggagcgatt
     6541 ttttcttgga ttgctaacgt tgtataagct gtcaatagat ttggcacata gtaataaaac
     6601 ccatgatgat ctaggcggcg atgtagcaaa agtatttcga tgtccacctg tgccgctaga
     6661 tcaatacaaa ctttgacacg agaatccaat gctgtcaccc accagcttaa taagcctccc
     6721 atagaaagac caatcgttgc gagccgttga gtatctgtgt cttcccttgt ctccaaataa
     6781 tcgatcaagc tgatcacatc ataaatacgc attccccata acgtttttcc tgttaataaa
     6841 aattgtttga acaactcgct ttcagaaata cctccgcgtt cttcaaatcc ccaagcatca
     6901 atcgaccaaa cagcatatcc cattcctgtc agtacttctg caaaagatgg agaagctaag
     6961 taaggagcac catggatcaa ttcacttttc ccgactgaaa aatctccgcc gtgagaatgc
     7021 aggtaaagga ccggcggaaa cggtccttct ttttctggat aagcatagag ggctggtact
     7081 gtttccaatt catttaagta caattgatag tattcaagca taaaaccgtt ttgtttttct
     7141 cggtataatc gttttccact aggggtatgt ctgctagatg gagcacctaa aaaatcattc
     7201 aactgcataa gcaaagtttt tacgaacaac cagttcatcc atattttctg attgatttga
     7261 ttggaatttc tcaatactga tttctttagt attttctaat agaaatgctg gtccctcgtg
     7321 attttcaatc gacatctggt taaaggaaac cttacccgca aagccaatat aaaatccttg
     7381 tctgctcatt tcctctattc ctgtcatcat agcaggaaca ccgggaactg catcttgggc
     7441 catcgagaca tcgatattgt caaatgtgat ttcttgtatg aattgttccg ctaatccata
     7501 aataaaacct gcagaagcat ggacattcct tgccgtgata ttcgaaaaat ggatccttct
     7561 gaatgctggt gtccgttcat ctatcggata agcctttttt tcccatacat acggttcttt
     7621 ccctcgcgga ccacagaaat aataaagatt cagtataaat ggacacatca cattatccat
     7681 gactatattg gatactcgaa tatcttcaac gatcccgcct cgacctcgtc tagatttcaa
     7741 tcgtattcct cgatcagttt cttggaagat acaatttgaa atggtaatat tacgaatact
     7801 accactcatt tcacttccta atacgacacc gccatgtcca tggaccatcg tgcaatttgt
     7861 gatcgtgata ttttcgcaag cgatcctttc gtacgtatct tcagtccctg ccttgattgc
     7921 aatacagtca tctcccacgt caatatggca attactaata cgcacatttt tgcatgattc
     7981 tggatcaatt ccgtctgtat ttggtgaatc cgctgggttc aagatcgtca agttatcaaa
     8041 cgttgcatta ctgcaaagaa tcgggttgat cgtccaactt ggtgactgga tcaatttaat
     8101 atctttcaca gtaattcgat gacagttatg gaaactcatc aatttaggac gaggataagc
     8161 gagattatct ggttcatttc taaaagtatg ccaccatttt tttccatttc catctaatgt
     8221 accgaaacca gtgacagaga tgttctctac attttgagca tagatacagg aagcataaac
     8281 ttttcgatgg actccttccc aacgtgaatg aacaacagga taatcttttg gatcgtcaga
     8341 gaatttcaga accgctcctg cgcttaaatg aagttcgaca ttgcttttta aaaataaagc
     8401 tcctgttaga aattcacctg ctggaacaac tacggtcccg cctccgtcac ttgctgcagc
     8461 atcaatggct tgttgtattg cttcagtatt caattcatca attgatgcgc caaatgtcaa
     8521 aatatcatac atatttataa tgcctccact tcaatcaatg cttgcaagaa tgcaccaacg
     8581 cctttttgat cattacaaat aattggttca ctgatgtagt aagcatatga accatctcgc
     8641 tgatctgctc cgcctaaccc tgctacttga cagtttttat tcaagttcag ccagccttct
     8701 tttgtttcca gaacaaattc atcaagtaat ccttgatgtg atttcttcac ttgtggaagc
     8761 cattcttctt tgttcagcag tcccaaacgt attcctttag ccattgcgta aacaaacata
     8821 ctactgccgg aagcttccag ataatttcct ttccgttttg tttctgttgt tacttgatac
     8881 catgtcccgc tgttttcttc ttgatatgga agtaatgcag ccagtgtctt ttctagaatt
     8941 tctttcagca aagaacaatc tgtttcttca tgaagtaatt cataggtatc tgctgcagcc
     9001 atcaaatacc agccaatcga tcggctccaa aaatgatggg atagacctgt ttttggatca
     9061 gcccatggtt ggaccttctt ttcatcccat gcatgaatca gcaaatttgt ttttggatcg
     9121 aatagatgtt tataactgat ttcaaactgt ctcaaaacat cttggtaatc tttttcttca
     9181 ctaaaattct ggataaattc agcataaaaa ggagaaccca tatataagcc atccaaccaa
     9241 atttgataag gataaatttc tttatgccaa aaagctcctt cggatgtgcg tggatggttt
     9301 gccaactgct gggaaaggag ttctgctgct tttttatatt tttcttcgcc tgtttctttg
     9361 tacaataaaa agaaaagctt tcctgtattt acatggtcga tattgtattc ttcaacgctg
     9421 tagccacgga tcgttccatc ttcttgcacg aaatagtcga tattgtcttt tatgaaatca
     9481 aaataaattt tttcttgggt tcgttcccaa agtaattgga accctttaag tacgacgcca
     9541 tagtcataag accattttcc tttataccac ttttcttcat ataagcgagg tgttctttcc
     9601 atgatacttt ttgctaattt ctttgaccag ttttccatta tttgctctcc tttaaaaatt
     9661 aaaataggat ttgggacaaa taccaagaag tatttgtccc aatcgtctat attactgtgc
     9721 cgctgctttg tatgcttcct cgtactcttg tgtgattcga tcgcctcctt gtgctttcca
     9781 gtcttctaca gcttttttga atccatcttc gtcaagctgt cccatgatgt attgcaccgt
     9841 agcatcaatg acgatttttt caatttctga tcctacttca ttgttcgtcg gtgaatccaa
     9901 tggcacagaa ggatccatta cagcaaattc ttcattttct ttgatcaatt gattagagaa
     9961 ttctttttca gggtctgtat ctttgaaaga gaacgtcact tcactaggac gagagcttga
    10021 taacggttgc acctcttgtt gccaaagatc catatcgata aattcgatag aaccatcatc
    10081 attcaattca taatgcgttc cttcgatacc gccagtcatc aatttataca catcttcttc
    10141 taaaagatca tcaacaaatt gaagtaattt cttcaattgt gcttcgtctt ttacttgaga
    10201 tttgggaaat gctagcaagc cgccaatacc atttccttct gaccaaacat gataaccgcc
    10261 gccttcacca tctgagatct tgtttacagg cactaattcc atatcatctt gaataccttg
    10321 acccatcgtc ttcagattct tgatatccac cattccagtg tagattccag ttcttccttg
    10381 tgcaaattgt tcttgctgat cagtcttttg ggtaactgca aagtcttgtg ccaaatagcc
    10441 attctcaaat agatctcttg aaaaattcaa agctttcata tactcttctg tatcaaattc
    10501 tggagtgaat ttgccattat cgtctacagc ccagccattt ggtgctccaa aataagaagt
    10561 gaaaagttta aagctggtat aacgaatatc actaggcgca cgatcgccaa aaccaactgt
    10621 gtcgtccaca ccatttccat caggatcgtt ttctgtaaat tgtttagcta cttcataaag
    10681 ctcatccatg gtagttggaa catctagtcc tagattatcc aaccagtctt ttcgaatgac
    10741 taagccagca cgagcgtagt ctttttgtat cggtacgccg tacaaggcac cgtcgatact
    10801 cgcagcttcg attctatctt cagaaatttg acttaaattt ttatagtctt ttaaatattc
    10861 tgatacatcc cagaatacgc ctgattttaa cgcattgcgg acagaagagt ttttcaacat
    10921 cgcaatttga agtgtaacga tatctcctag ttcatcagaa gccaatgcag ttgttaatct
    10981 ttcttctttt gaagcatcag gaatccagtt gaatgtcagt tccgtatcgg ttttttcttc
    11041 gatcaagttc acaatcgtgt ctgtaggcgg tgaagcatga tgcaaaatat ttaaccaagt
    11101 gattttagct tttccatctt ctgtctctga agaactgtta cttgaactgc tgccgcatgc
    11161 ggcaagtgct agaattcctg caaaactaag acctaatttt acgagcgttg cttttttcat
    11221 aaattttctc cttcactgtt ttaataatat ttttgttttc ttctgttttt tctgtatatg
    11281 agttcttcca ttccaaatgg caaaatatag aatttgctgt ccacgtgact gcggaaaaga
    11341 agagactgcc accgataaag gttaaaacac ctggcattgt ttggatgatc atcagataaa
    11401 tccctaccat acttaatatc aacactaatg agtaatgaag atgtgagaat ccgaataaaa
    11461 atgccatttt cattttgaaa aaatatcctt tccaatcata atgagccatg attgggaaaa
    11521 tatagagtgc tgagagcaca tatatgaacg cagcaatata caaagcgatc ctaagatacc
    11581 aattgtgtat gaaagaaaga tccacaagta aaacaatccc gatggcgcca tatatccagc
    11641 tcatcagcaa ggtttcacga tagttttctt taaaatatgt gaagtacgtt ttcgtcaaag
    11701 gaaacgtttc ttttgagcgc agccattttc taatcactgc aaccatcgca tacgatgcag
    11761 gaccgaaagt aaaaactccc aaacctgcga tgactaaacc gccccacaga aaattcaaat
    11821 aaaccaactg taaaatacga tttagccttc cgtttatttt atctaatgac tgcagcatat
    11881 ctttccctct ctttctatct tagtaaacgc cgtcttctcc tagctttttg gcaatcttat
    11941 tagctaaaat taccataaac aatcctacga cccctttaaa caatcccatt gctgtactga
    12001 aacttaactg tccattcttc aatccagctg tataaatata ggtgtcgaaa atttctgcaa
    12061 cgcttcgatt caatgaattc aataacaagt acatatgctc gaaccctaag tctaatgtct
    12121 gaccgatttt tagaatcagc aaggtaatga ttactggacg aattgccggc aatgtaacat
    12181 gccatgtttt gcgtaagcgt cctgctccat ccatttctgc agcttcatac aactgtgtat
    12241 cgactgcagt tatcgctgaa aggtagatga tcgtagacca gcccaactct ttccagatca
    12301 cttgtccgat atatactgtg cgtgtccatt caggggatgt tagaaaactg attttttcaa
    12361 atcccattgt tgcaaggaat tcattcaata cccctccgtc aacattcaaa aatacataag
    12421 taatcgaaac aatgattacc caagacatga agtgagggat gtaaatgatc gtttgaatcc
    12481 cgtttttcaa tcgtttgttc gttacttcat ttaatagtaa agacaaaatg attggcatcg
    12541 gaaaaaacac gaagatattt aatccgaaca agatcaacgt atttcttaat aatgtaaaga
    12601 atgtcggttc tgtaaacaaa cgaatgaaat gtttgaaacc gacccaagga cttcccatta
    12661 ttcctaaaaa tggttgataa tcttgaaaag caataacgag tccccccata ggaagatacc
    12721 taaaaatcaa gaaataaagg actcccggca gaatcatcac atataaaaat ttattcgttt
    12781 tgattcctgc aattattttt tttcgtcttc tgagcttttc ttcttttttc gatagcgtca
    12841 gttgaaatgg ttcttgtttc tttacacttt ccataagccc ctccttagtt ttgtgaatgt
    12901 ttgctgctta tgacttcatg atagataact atctgaatta cgtatataat catttttacc
    12961 gtaagtaaaa acgcttacat agcaatgatt tttgtgctat acaaaacgat aaagtgtatt
    13021 ttttcacaaa taaactacgc atagcaaacc ttctattttt ttctatctga cacttagcat
    13081 aggaaatgat tatatacgta atctttcacc gatgataaat tgaacatata gaaacagaaa
    13141 catttaattt attttgagga ggagcctatc tatatgaaaa agaaagtctc tataggagaa
    13201 agaagtttta cacttttcaa caatacgttt ctaattttgc tagctttgat ctgtatcgtt
    13261 ccttttttaa atatcatcgc tacatctttt gcttctacac aagaagtcgt cgcgaaaaag
    13321 tttatccttt ttcctactac cttttcttta gacgcgtatc gctacattct ttctacgccg
    13381 accattttta gagcacttgc tgtttcgatt ggtgttacag gtgtcggaac gatcgtcagt
    13441 atgtgtgcaa cttcactgat ggcctacggt ctttccagaa agtatttgtt tggtcgaggt
    13501 ttcgtcaact ttcttgttgt attttctatg ttgttcagcg gggggatgat tccaacgttc
    13561 ttagttgttc gttctctagg gctggtcaat tcttactggg ccatgatcct gcctgtggct
    13621 gttaatgcaa tgaatatgat cattatgaga aactttttcc aagccttgcc ggatagtttg
    13681 gaagaatcag caaaaatgga tggttgcaca gattttggtg tatttttcaa aattatgctg
    13741 ccattagcgt taccgtctat tgctactatc tcgctttttt atgctgttac ttactggaac
    13801 acttatatga ctgctatttt gtatatcaac gattcatcta aatggccgat acagattctt
    13861 ttacgccaaa tcgtcattgt atcaagtggg atgcaagcag aaagtagtgc cgttgatgtg
    13921 attccaccag cacaaacagt taaaatggct gttatcgtta ttgcaacagt gcctatgctg
    13981 attgtttatc cttttgttca gaaatatttt gtcaaaggag cactcgtagg ttctgtcaaa
    14041 gggtgattat gtactgaacg caaaatggta gttgtgtcag aagtggtcag cttcaagaaa
    14101 taagaaggca tttacgaaaa ttgcttctta aatttttgtg aaatttcggc ttatttttcg
    14161 aagaatctac tcctggaaca ccgtgtatca gaggttgtga aagagacgtt cagccttgag
    14221 cgatgataaa aactctcaca aacattgcga cgagtaaccc acaggagcaa taagaagcga
    14281 ttttgaaaaa tagcttccca tatttcacaa aattatgcct tattgtcgat agcctgtctc
    14341 ttgaacgcca tttattcgta aaaaagaact gtgacaagac ttttgtcaca gttctttttg
    14401 cgtgcttagc cttgaaattt aactatccat aaatacaaat actagggtta ttctaacttc
    14461 ttttattgct tgaatcagtc cacaagaaaa acagcctctt gattcacaaa tatttttttc
    14521 gatattctcc cggcgtaatc gattccttct ttttaaagaa acgaataaaa ttttgcggat
    14581 ttcgatattg caatctttct gcaatctctt ttacggagag gttagtctct ttcaaccatt
    14641 tttttgctac ctctaaacga tgattttgaa caaaatcacc aaatttttct cctgtttcct
    14701 ttttaaatac attacttaaa tagttagggt tataatgcaa cctatcagcg atcacatcta
    14761 aagacagatc acgatcatat tctgtgtaga cgatatgcat aatattctcc gacaaatttt
    14821 taaattcatg atcagtgatt gcttgagagc attctgtgat tggcagaact aattgttcta
    14881 aaacaagttt ttctagttct ttgggatgat aattgttcag tgcttttaca taaatttgtt
    14941 tcatgttatc aaagtttttt gaatctactc ccaataactg tcctaattga acgagttcat
    15001 tgatcaagcg gaccgaaact acttcccgac tcaaaggatt tttattttgt gcaaacaatg
    15061 catcaatcaa ttcatgtact aaattcttca cttcctgatt tccagaacga atggcttcaa
    15121 acaatcgatt ttgttcttct atcggatatc taataagtgt tttttcatga gcagcaggaa
    15181 cgatttcctg ataaaaaata attgaacttg gaccagtatt tacacgataa tacagggctt
    15241 cttttgcccg atcaaccgat tgcttgcttt cattcagcgt ttcgaaccgg ttactgatgc
    15301 ctatactgat agttaaattc aagtattctt ttacacgttt ttgaatgagt cggcaatact
    15361 ccatgacttt tttgcttgct tctgcatcat ctttatccat tcgataaata gtggcttgca
    15421 tttcttcatt caaaacgatc ggcagcattc gattttcctt tggcacaatt tccgtgatca
    15481 tgttgttgat cgctaaaagt agcaaatcac gctccccacc gtgactatct tctaacagat
    15541 cgatttgaac caaagctgta taatagcagt cattttgaga actataacca aattgctgca
    15601 accgctgatc tagttctcgc ctttctactc gatttctaaa taaactaagt acaaatagcg
    15661 tttccagctg tggcttttgg gtaactaagt gtgcacttaa cgcttctttt tctcctatga
    15721 tccgactgac tgattgagcg accaaatcta gttctttccc actgaatcct tttgatttaa
    15781 gattcagacg ttcctgtatt tgagaaatcg gacgggcaaa tctgtccgaa aataagtagg
    15841 aaagaagacc aatgagtcct accagtgtta aagacactgc aactagcccg attctcagat
    15901 tgcggatcat tgcgccaatt gcactagagt caacttctaa tctgtaaatc caattattat
    15961 aatctgattt cagtactata tacgttttat ctttttctct tatcactttc agctgattgt
    16021 aatctagatc cacgtccgtt aatacagttg gttgctcttt attttttact gtctgagtag
    16081 taaaaaggtt gccaacttcg ttttcaatag aaagtatgtc ttctccactt tcaccaacaa
    16141 cttgttctat tgtatgtttc tctatcgtag caattcccaa tgcatatttt tctttttcaa
    16201 acattggcaa agaaatcacc atttcgattc ctttgttttt cttttgccaa aataaattat
    16261 ttttgttatt tatgtacttc tgttgatact tttcaatttc ctcttcggtc agttgactca
    16321 acgatccatt gattactccc catttaccag ccaaactgac taaatcataa ttattatcat
    16381 ccataacaat cgtttctata aaatttagtt ctttcctgat ttcagaataa gtggagtaat
    16441 ctgtatatgt cagcggatgc ttgaatatgt ccgagaaatt tttggtcgaa ccatacgtag
    16501 aaaacgaata ttcaatcgtt ctaattttcc attcaacgtt agttttaact tgcctgagca
    16561 aattcttttt gtctgttaaa taactcgatt cgatcgattc ataggtattc tggtaaatga
    16621 ataaactaaa tcctatgaca agtacggctc ctaataaaaa catcggtaaa aatattttat
    16681 aaaatactgt acttttcttc attttcttcc tctcctcctt ttaaaataaa aggctttcat
    16741 ttttctgtaa aattgatact catctaatta aatgagcatt ccccgctttc aagaatagca
    16801 taattaaaaa taaaaatcca agtaattttt aacgtgtgca atcttttcat atcaaagatc
    16861 tattgactta tcgtagttat cgtaatatga tgaatgcgtt aacaaaaagt gaaaaagtaa
    16921 ggaaaatgat ggaatggtaa cgatcaaaga tgtcgctaaa gcagctggcg tttctcatac
    16981 gacagtttct cgtgctttga ataacagcag tttaataaaa aatgaaacaa aacaaaaaat
    17041 acgacaacta gctaaggaaa tgaattattc gccaaactat agtgcaaaag gtttagtgaa
    17101 tcaaaaaaaa tatctaatcg gactattttt ctcaagtttg cataaaggaa cttcatcaaa
    17161 tttcctagca gattccattg caggcatcca ttcagtatta gacacaaatt acagtttatc
    17221 tgttgaaagt atcgatgttg ttgatttaac agaaatcaac ctgcaacgct acgatggact
    17281 gatcgttatg agtcaaagtg atgatgatca gcctttcatc gactatctca aagaaaaaga
    17341 atttcctttc gttgttgtca atcgtcacat caatgatcca gcggttatca acgtcactgc
    17401 cgatgatgcg gccggtgtta tgcaggcagt agattacggt attggatgcg gacatacgaa
    17461 gatcgcttat attggcggaa agaaaaattt tcgttcgaca aaagaacggg aaaaaggggt
    17521 attgaaaagt ctaaaaaaag cgagcatccc tgtcaatcca gcctattttt taacaggaga
    17581 ttacagctta gaaagcggcc tccaaaacat ggaataccta ttatcacttt ctgacctgcc
    17641 tactctggtt ttttgcggca acgacgacat agcgattggt gcattgagag ctgtacattt
    17701 agctggcctc cgtgttcccc agaacatttc tctgatcggg ttcgatgatt ctccagtcgt
    17761 ctcctatctc aatccgccgc ttaccaccgt acgtaagcct ttgaaagaaa tgtgtcaaga
    17821 aggaacaaag cttttactag atttgatcga aggcaggacg atcgaacaat ctaaaattga
    17881 gctctccacc ttgttgatcc ttcgtgagtc gatcaattac ttgtaaacta caagtaattt
    17941 tatataaaaa tgttaacgtg tgcataacac agaaaggaag tttatatgaa aaaactgaca
    18001 aaagaattag tgaaaaaaaa cgaagcacct gtaaaagttc ttcagtttgg tgaaggcaat
    18061 tttatgcgtg gatttattga ttggcagatt cagcagctga ataaccaaca attgtttaaa
    18121 ggtaatgttg caatcattca acctttagaa caaggtcttg gaaatatgat gaaccagcaa
    18181 gataatttat atacggtcat tttacaagga ctcagcaatg gcgaaatcgt tgatacttcc
    18241 gagattatca ccgtagtaga acaaatgatc aatccttatg atgaatggga tgcttatcta
    18301 tctctagcag aaaatgacga cttggcctat atcgtctcca atacaacaga ggcaggaatc
    18361 caattcgtgg agactgataa tttaacagat actcccccac agagcctccc gggcaaactg
    18421 acagcctttt tataccgacg tttcaaatta aacaaagctg gctttaccat cattccttgc
    18481 gaactgattg acagaaacgg cgaacgtcta aaagaaatca ttttacgcta cgcagaaaaa
    18541 tgggatttag aacctgcttt tgttgagtgg ttagaaaaag aaaatgtttt ctgttgtagt
    18601 ttagttgatc gcatcgtccc aggttatcct cgcgaagatg caggtgtttt caatcaacgg
    18661 cataactatg aagatcaatt gatggtcaaa gcagaaccat tcatgctttg ggtgatcgaa
    18721 gggcctaaag aattggagaa tcaactacca ttcaaacagg ctggcttgaa tgtcatcatc
    18781 acagatgata tgactcctta tcgtgaacgt aaggtgcatt tgcttaatgg accgcataca
    18841 gcgatggtcc ctcttgcttt gttggcggaa ttggaaactg tagaagaagt aatgaaagat
    18901 cctttattct ccgcatatgt tgatcagtta ttcaatctag aattgattcc tatgctttct
    18961 ttacctaaag atgaattagc catttatgct gaccaaatca aagaacgttt cttgaatcct
    19021 tttgcccatc acaaattaga agcgatttca ttaaatagtg tttcaaagtt cagcacccgt
    19081 ctgcttccag tgttcaagaa gtatatcgaa gaacaaaatc aggtaccacc gttaatcact
    19141 gtttcactag ctgcgctact tcttatgtat cgaggcgatc aagtaaagcc acatgatgat
    19201 gaaaagacga taagcgaatt tactgatgct tggtctgata atggaacagc tattcctcga
    19261 ttattgcaaa acgctgcgct ttggggagaa gatttgtctc aaataccgaa tgtgacagat
    19321 acagttcaag aaatagctgc aaagatcgat caaaaaggtg tccggtcagt attacaagaa
    19381 atgaaaggaa cgatggtcca tgcttgaaac cctgcaacca acaatgaaat taaatcgacg
    19441 agacagtgtg gtcgtcgcat taacccctat tccaaaacaa acacaactga cgattgatca
    19501 gcaaacgatc accactcttg aagatatccc gcaaggtcat aagattgcat tgggtgattt
    19561 gaaagctgga gacaacgtga tcaaatatgg ctatccaatc ggtcatgtga cgacagacgt
    19621 aactgccggt caatggctgc acactcataa tgtaaaaacc aatctttctg gtgaattgga
    19681 ttatcgatat gaaaaagatg tccatccaag tcattacctt tttgagaacc gaacatttca
    19741 aggttacttg cgaaaaaatg gaaaagtagg gattcgtaat gatttgtttt tagtacctac
    19801 cgttgggtgt gtgaatggca ttgctgaact gatcgtcaaa cagttcaagg aaaaacatcc
    19861 agatctggga cagtttgacc atattacgat tttaaagcat ccttatggct gttcgcagct
    19921 tggaaaagat catcaaaata ctcgagaaat tttagcggac gcagtaaatc atcccaatgc
    19981 cggaggagtt ttagtatttg gattaggttg tgagaacaat acagttcctg aattcaaaaa
    20041 aattttaggt gaatatgatg aagaacgagt gaaatttcta gtggctcaag atgtttacga
    20101 tgagatcgaa caaggtgtgg ctctattaga agagctttta acagcggcgg aaaatgacca
    20161 gcgcatatca gtcccgctct ctaagttgaa tgtcggtttg aaatgcggcg gttctgatgg
    20221 tctatcggga ataactgcta atcctttact tggtgcattt tctgactact tgattgctca
    20281 aggaggcagc acgatcttaa cagaagtgcc agaaatgttt ggcgcagaac aagtattgat
    20341 ggcacgagcg gaaaatgaag aagtttttga ctcaatcgtt gacttgatca atgacttcaa
    20401 acagtatttc ttgtcttacg gggaaccagt ttacgaaaac ccatctcctg gtaataaagc
    20461 aggtgggatc actacattag aagataagtc tttaggctgc acgcaaaaat caggaagctc
    20521 tcctgtcgtt gatgtgctac aatatggcga aaaaatcaga aaaccagggc ttagtttatt
    20581 gcaagctccc gggaatgatc ttgtagctgc ctctgctctt gcttcatccg attgccagtt
    20641 ggtcttgttt accacaggaa gagggactcc gtttggctca tatgtcccaa cggtcaaggt
    20701 atctacgaat acaactcttt ttgaacgcaa aggtcattgg atggatttta atgcaggcgt
    20761 cttattagaa caaccaatgg aagtaatctt agaatcgttt gttcaaaaaa tcatcgctgt
    20821 agctagtgga gaagaaacga aaaatgaaca aaatgacgtt cgagaaattg ccatttttaa
    20881 aaacggagta acactttgat tttcataaaa cagtataaaa agaaagaagg aagatttatg
    20941 tttttatctg atgatttttt attaaaagac gattgggcga aaaagcttta ccattcctac
    21001 gcaaaaaaca tgcccattat tgattatcac tgtcacttag atccaaaaga aatttacgaa
    21061 aacaacaatt tcagcaatct gacagaagct tggttaggag gcgatcatta caaatggcgc
    21121 ttgatgcgag cttgcggcgt gcctgaagaa aaaatcacag gaaatgcttc tgattttgaa
    21181 aaatttttag cttggtgtca aaccgtacca aaaattatcg gaaatccgct ttacagttgg
    21241 tcacatcttg aattgaagcg tttcttcggt attgacttgt tgataaatga agaaaatgca
    21301 gagaaaattt ggacacaagc aaatgaaatt ttggcagctc ctgattttcg ccgtcgggaa
    21361 atcgcaaaaa aatcgaatgt agaagtgatt tgtacaacag atgatcccgc agacgacttg
    21421 cactatcatc agttgcttgc tcatgaagaa ccagatttga aggttctacc ttcattccgt
    21481 ccagataaag cactcaatat tgaaaaagaa ggatttaatg catgggtcac actactggaa
    21541 gaagcagctg accaaacgat caccacctat caagaactga tagaagtctt agggcagcgg
    21601 atagaatact tccatcaaca tggatgtcgt gtttccgacc acgctttaga catcttacgc
    21661 tataaagaag cggatgaaac agtattagaa gagatttttc aaaaagcacg agctaacaaa
    21721 ctattatctt cagatgaaat cgatgcttat cgtacagaaa cactgataca tttgacccac
    21781 ttctatcatt ctcataattg gacgatgcag ctgcacatcc atgcttatcg taactgtaat
    21841 acacaaatgt tggaaaaatt agggcccgat accggctatg atggaatgaa tgatctttct
    21901 ttgactattc ctctgcaaaa attattgaac cgtgcagaag aaacggatca gttgccaaag
    21961 acgatccttt attcattgaa tccgaatgac tatcctgcga ttttagcttt gatgggctgt
    22021 ttccaaaaag aacagaatgg aaaactgcag ttaggttctg gatggtggta caacgatact
    22081 cgagctggta tgcgcgatca attgacacaa tttgctgatg gtagtgcttt ggggaatttt
    22141 gtcggcatgc tgacagattc aagaagtttc ctgtcctaca ctagacatga atatttccgt
    22201 cgtgttcttt gtgaattttt aggcgaaatg gtcatgcgag gagaagcacc tgaagatgaa
    22261 caacttttag gaagtctagt tcaagcgatt agttatacaa atgctaaaaa ttactttggc
    22321 ttttttgcct aattgattaa tagtaacagt aacgaatcaa aaacaggata gtgaaataac
    22381 ggaatccgtt tatccacgtt ttagagaaga agacggacca aatttgctcc gtcttcttct
    22441 cttgctgttc tgcttcattg cctataaacc tttatgaaag aagagataac atgcctacta
    22501 tccatattgc cggtgattct actgcggcag aaaaagatga aaacaagcgt cccgagacag
    22561 gttggggaga aaaaatctct gcttattttt cacccgagat ccagatcaat aaccaagcaa
    22621 agaatggacg cagcaccaaa tcatttatcc aggaaggaag gttagcccgt ctagcttcac
    22681 agtttcagcc gaatgatttc ttatttgtac aatttggaca taacgaccag aaaatccaag
    22741 aagaaagagg tacgcttccc ttcggtagct atttaaaaaa tctgcagata ttttttgaat
    22801 gcgcaaaaaa agcgaacgtc caaatggtct tactgacatc tgtgacgcga cgagattact
    22861 tagaaaacgg aacattgaat ccagatatac ttggagacta tccaaaagcg atgcgtgcat
    22921 ttgctgagaa acaccatatt cctcttttag atgtgttctc aagatcacaa gaattgttcc
    22981 agaccttctc aaaagaagag acaaaaaaat tctacctgca tcttatacct gatacttgta
    23041 aaaattaccc agaaggacta aaagacaata cccactttag tccagaaggc gctgataaag
    23101 ttgcccgaat aattgttgaa ttgataaaag aatcccgtct gcctcttgct aattatttac
    23161 aaaaaggagt ttgagatgtt tccattaaat ttaggcatcc gtgcccacga tttagatgtt
    23221 cgaaatcgtg aagaaatcgt tcaaaaagta tcacagcttc acttgtccca tatccaattt
    23281 gcaccacaaa aagcatttcc tgaacaactt aaattaactg acatgacact tgggagtgct
    23341 gcctattttg gtgaatactt ccaaaaaaac aatatcgaac tttccatttt aggatgctat
    23401 atcaacctat ccagcaaaaa tcaagaagtt cgtgcacaag cattggcaac tttcaaacag
    23461 caaattgctc tgtgctctgc ctatcaagcc aagatgattg ctacggaaac cggtagtgtc
    23521 tctgtcggtt acacaaaaga aaattttact gaagaagctt atcagatcgc cagaaattct
    23581 atcattgaat tagtcgctca tgcagaaaat tttggcgtaa ctgttgctgt tgaagcaggg
    23641 atcaatcatc caatctataa ctatcagtta gctaaacgtt taatcgatga agtagcttca
    23701 ccaaatttaa aaatcatttt agattgtgcg aatttgatgc ataaagaaaa tcatgctgat
    23761 caggaaaaag tgatccgagg tgccttggac aatttacatg gttatattac agcgcttcat
    23821 ctcaaagact atatcatgga aaatggaaag atccgcatgg tacctgttgg caagggctgt
    23881 ttagatttcc gcccggtact gcattatatc aaatatgagc gtcctctaat gtacgccact
    23941 cttgaagcta ctcctgagat ttatgttcct gaagcaatag aacacttgca gtttatttac
    24001 caagattgtt aaggagaaga ttatggagga acgaatacag aaaagttatc aaaaatatca
    24061 tgcaaatgct tttcagacat tgctgcatct ttatcaggga aattattggc gttttgccat
    24121 gtcaggactc ttttttatta tcaaacatct tccatcttgg ttaatgccga tcgctattgc
    24181 caatgtaatc aatgcagctt catcgaaaga ttcttctcag ttgcaggcca tttatttcaa
    24241 cgcttttttg atggcttgtc tgattggtca aaatatattg acgaattatt ttcatgtaaa
    24301 ataccatagc acttcgatcc gtcaagtaga agctggacta agagcgaccc ttatcaagaa
    24361 aatccaagaa ctatctattc cttatcaaaa agaattgcaa tctggacgaa tccagtcaaa
    24421 gatcatccgt gatgtagaag ctatccaaac gttgtcctct caagtatttg tcagcggatt
    24481 aaatatcgct gtcaatttat tcgtagcttt aggtatcacg ctttataaaa gtcctgttgt
    24541 tttcatgttt ttcttattga ctgttcctct cgccgcattc gttactctct ttttccgtaa
    24601 aaggatcaga cagtccaact cagacttcag gcaagaaatg gaagagactt ctgcaaaagt
    24661 gatcgaagtg atcgaattag caccgattgc tcgagctcat gggttagaaa aacacgaaac
    24721 aactcgctta aataagcatt ttactcaaat ctatgaaaaa ggattccact tagatatcct
    24781 gcaatctgtt ttcggatcag tcagctgggc tagttttcag atatttcagc tgatttgttt
    24841 gattttcact gggatattag ctttacaagg gcgcatccaa cccggcgatg tcgtgatgta
    24901 tcagacgtat ttcacgacga tcgtcaattc cgtttctgga atgatcacgc tgattcctac
    24961 gatttcaaaa ggcatggaat ctatcacttc tattggcgaa ctgttgattg ctggcgatgt
    25021 agaaacttat cacggtaaga aaaaaatcca acatttggat ggaaattttc gttttgagga
    25081 tgtttctttt ctttatccgg actcactcaa accgattatc gatcattttt ctctagaggt
    25141 gaaagctgga gaaacaattg cttttgttgg gccatctggc tctggaaaat ctacactcct
    25201 taacttattg attggattta tccagccgac ttcaggaaaa atttttctag atgatcagcc
    25261 tttggatgaa ctggatatgc gaagtgtgcg aaattattta gccgttgttc ctcaaaccac
    25321 tttgttgttc tccgcttcga tcaaagagaa catcacttat ggcttgaaaa acgtatcaaa
    25381 agaacgttta gatgaagtca tcgaagctgc gcaactttct tcactgattg accagcttcc
    25441 tgatggtctg gataccttag taggcgaaca tggcaacaag ctgtctggtg gccaaaagca
    25501 acgtatctcc attgcacgag cgttgatacg tcagccaaaa atcattttgc tagatgaagc
    25561 aacctcagct ttagataacc agtcagagaa aaaaatccag caagccctga attacctgac
    25621 agaaacaccc actactttta ttgtggctca tcggctttca acgatcaaag aggcagataa
    25681 aatcgttgtg atcgatgaag gaaaaatcgt tgaaataggt acttacgaaa aactgttagc
    25741 tcaaaaaggc tatttctacc gcttgaaaaa tgaatgacaa aaaataggag ctatgacaag
    25801 acttttgtcg tagctcctat ttctgaataa acggtgttct cctgccaact cctcggtcac
    25861 aagtcacaat agtcatcaaa catgagaagc tgttttttga aattgttcct tgtaactcag
    25921 agttaaacgg taggctcacg acctcttggt aaatggtgtt caagagacag cctatcggca
    25981 ataagaagca attttgtcat cgctcaaggc cgaacgtctc tttcacaccc tctgttgttt
    26041 cataaaatag ttttattcgc tgtaaaggtc gcgaactggt gtcatgatga tacggttcaa
    26101 gtcattgatg attgtactga agccttgctc tttttgcatc aagtcattga ttacttcttc
    26161 tgattggatt ttcacagcca tttcttgtgc tttcattgca tcttcgtcgc tgaactcttg
    26221 tccactcatt tgtttttctt gtaattcgat ttgcatcgct tggaagtctt tgaacagttt
    26281 gtgtgcttct tcgtttgctt gtactttctc gtacgcttct ttcaacgctt tgaattctgg
    26341 taattcacgg atctcacgtt cgatttgatt cgcgctatcg tagatgtttg acatgttatg
    26401 ccctcttttc ttttagtagt tactctctat tgtaacatgg ggttattcgt tttcaaaggg
    26461 gctactctcc ttgatatttc cgccagaggt ctttagccgc ttcaccaatt ccctgaacgc
    26521 cttctttcac catatcgccg aagtttttca gcccttcttt tgcttgttca ccatattgat
    26581 tcaagttatc ttgccaattt ccagactctc cattagaatt tcctgtgttt ccaacttgat
    26641 ctgcagctac cacttgtcct cctgtggcat aggcatctgc tacactaaat cctgtttttg
    26701 aagtatacgg aaggatgcct tctgccgctg atttgaagac tttcccgaca acatttccac
    26761 ttgtcccttc caaatagtgc agcttgctgg attcttcgta acccatccat gtcgaaatca
    26821 ctacgtctgg cgtgtaaccg atcatccatt ggtcatttgc ttttgtggca tcgaaattag
    26881 tttctgtggt tcctgttttc cctgctatcg tataacctgc aggattcgca gcagccgctg
    26941 ttccatttga aaatgtacca agaagcatgc tggtcatttc atcggctgtt tcctttgata
    27001 tgacctgttt tggttttttg tttgtgttat cgacgatcac cgctccagtc gaatcaacga
    27061 ttttcgtgat aagatgcggc gtatacatct gaccatcatt ggcaaatgca ctgtaagcac
    27121 ccgccatgat caatggagat actccttttt ccagtccacc aagggctaag ccataatact
    27181 tatcagattt tgctaaaggc aggccgaatt cttctgcttt atcatatcct ttttgtaaac
    27241 cgatctcatg caaaagccat acagtaggca aattcaaact ttgtgcaaca gcttgataca
    27301 tcggtacttc tccttggtat gtcccgtcaa aattatgcgc atcgtaataa ctttgcggtt
    27361 cgtctttcaa gatactggaa ggcttgtatc cagcttccag tgctggtgta tatacgctta
    27421 atggtttgat cgttgatcct ggtgaacgac gcatctgtgt agcaaagctg aatcctctaa
    27481 agacatgatc tcctcttcgt cctactagtg cttgtacacc acctgtattt ggatctaaag
    27541 ctactgaagc agattggacc atggcaccat ctgaagcatt tgctgggaat aatgcactgt
    27601 tttgataagt agcttctaat tgcttttgat aatcctgatc taaagctgta tagattttat
    27661 agcctttatt catgacatct tcttcatcaa gaccatactc gttaactgct tcatcgatca
    27721 ctgcatcaaa ataataagga taacgataac cagaatctga attatcgtat gtgtcattaa
    27781 gatacgcgct gatatctgtc tggctttctg cttgttcttg ttcttttgtg atttttccgt
    27841 tttcttccat tacacttagt actgtgtttt tccgatcatt cgcatttttt gggtgatcga
    27901 tcggattata aataccagga cctttcagca ttcctgccaa agtagcagcc tcagacagcg
    27961 taacattcgt tgcatctact ccaaaatact ttctggaagc gtcttggaca ccccataccc
    28021 cgttgccaaa ataggcattg ttaagataca tggtcaagat ttcttctttg ctgtacttct
    28081 tttcgatctc gattgccaaa aataattcct tcgcttttcg atcaaacgtt tggtctaatg
    28141 tcagatacgc atttttggca agctgctgag tgatcgtact tcctccgccc cctgatgttc
    28201 cgccagacaa gaccattcgg actgctgcac gggcaatccc tttgatatca aacccgtgat
    28261 gctcgtaaaa attcctatct tctgtcgaga ttactgcatc ttggatatag ggagaaatcg
    28321 cgtttaattc tacatatgtt cctttttggg cgtacagtgt gccagcttct tcgtttttct
    28381 tatcataaat gactgtcgat tggcttaatc ctgattttaa cgtttcgaca tttgcttgtt
    28441 ttgctaaaat aaagaggtag atacttgtca ctaagacaca gatcaaacca atcagcaaaa
    28501 tcaatttgtt cacatgatat tttttccaga tgcgctttcg ccatcgatgg aattgtccga
    28561 gatatggtct gatccaacgc caaaaactga tcaaagcagc ttttatcttt tctaaaatgc
    28621 tttgaaaatc catttgcttc ttctcctgtc tatttgattc aatcgtctta ttttaccata
    28681 gtgaaagagt gttttcttcc tgcttaagtt gtagttcaat atgttttttt acgttagtgc
    28741 aagtagagta acataagaat gaacagaaaa atgtaagttt tttcttaaat atgttttaaa
    28801 aatggtacaa ttagtctgcg attgcactga aaggagtaca cttgtgaaaa tcacgatcac
    28861 tttacctatg aaccatcctg aaacaacaat tcgcgacttg cttgaaaagg aatggttagt
    28921 tcctagaaag gttcgacact ttttgcgtat acgtaaaaac gtttggatca atcaacaacc
    28981 tgctttgttc catcatgaag taaagggcaa tgaccagatc acattgttat tcgaagaaac
    29041 agattataat tatcaagaag ttcaattagg agatgcgaag cacatccgtg ttttatatga
    29101 agatgagcat ttgattatag tggataagcc tgcaggaatg aagactcatc caaatgaacc
    29161 gacagaaaac agtaccttac tcaatcattt agctgcttat ctcgacacta aacagcaacg
    29221 accttacgtt gtccaccgct tggacaaaga aacaagcggt gccgttttat ttgcaaaaaa
    29281 tccatttgtc ttgccgatac ttggaagaat gttggaacaa aaaatgatct atcggagata
    29341 tcaagccatc gtatggggag aaattgccaa agatatgacc atcaccaata aaattggacg
    29401 agatcgccat gatcggagaa aaagggtcgt agatgataaa aatggtcaga gcgccttgac
    29461 acatgtcact gtcaacaaag tactttctga acaaacacaa gtctattgtg ttttggaaac
    29521 cggacgtaca caccagatca gagttcattt agctcataag ggacatccga tcgttggaga
    29581 ccctctctac caaaatagaa cagctgaacg actgatgctc catgcattag aactgcacat
    29641 gatccatcca tttactcaag aaaaaatagt cggtaaagct ttacctggat tatgggaata
    29701 gaaataaaaa agaacagagt cttatcagca gataatgata agactctgtt ctttttgtgt
    29761 aatttgtctg cttacttttg atgttcgtgt tctgctaaca gcaaactacc gataatccca
    29821 ctttcattcg gcagtcccca acgaacgata tattcactta cttgaggtgt ttccatataa
    29881 ccacccatca attcaacaaa ttgctgacgg attttttgaa ggagatgatc ctgattcatt
    29941 accccaccgc ctaaaataat cttttcaggt gctaacgtca acgtatagtt gatcagtgct
    30001 tgagcaatat aataagcctg aatagcccaa acaggatgat cttgcggcag atcttgccct
    30061 ttgatccctg tgcgtgcctc aatagacggt cctgctgcta atccttctaa acaatcttta
    30121 tgataaggac aagtaccttc ataatggtca tctggatggc gtttcaccca aatatgtccc
    30181 atttcaggat gtgcgatccc tgaaaaaatt tcttcattga tcacgacacc tgcaccgatt
    30241 cctgttccca cggtaaggta aatacaacta ttttttcctt gtgctgctcc ttgcttcaat
    30301 tccccatatg cagcagcatt gacatccgtt gtccaagcag caggaatatt gtagcgttcc
    30361 ttgattgtgc caagaaaatc gaattgtgcc cacccttttt tgggagttgc caaaacgtat
    30421 ccgtaattat cttttgtttt gtcgatacca atcgggccga atgagccaat cccgattccc
    30481 ttcaattcat aacgatcaaa aaattcaaat acctgatcca atgtttcttc tggtgttgtg
    30541 gtagggatac ttaatt
//
LOCUS       DACDIE010000031        28564 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.031, whole genome shotgun sequence.
ACCESSION   DACDIE010000031 DACDIE010000000
VERSION     DACDIE010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28564
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.031"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(101..1045)
                     /locus_tag="IV250_001523"
     CDS             complement(101..1045)
                     /locus_tag="IV250_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8768813.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            1266..2480
                     /locus_tag="IV250_001524"
     CDS             1266..2480
                     /locus_tag="IV250_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8768814.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            2502..4517
                     /locus_tag="IV250_001525"
     CDS             2502..4517
                     /locus_tag="IV250_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HAP8768815.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(4600..5973)
                     /locus_tag="IV250_001526"
     CDS             complement(4600..5973)
                     /locus_tag="IV250_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP8768816.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(5999..6283)
                     /locus_tag="IV250_001527"
     CDS             complement(5999..6283)
                     /locus_tag="IV250_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8768817.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(6285..8300)
                     /locus_tag="IV250_001528"
     CDS             complement(6285..8300)
                     /locus_tag="IV250_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP8768818.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(8479..8784)
                     /locus_tag="IV250_001529"
     CDS             complement(8479..8784)
                     /locus_tag="IV250_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HAP8768819.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(8873..9904)
                     /locus_tag="IV250_001530"
     CDS             complement(8873..9904)
                     /locus_tag="IV250_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAP8768820.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            10111..10986
                     /locus_tag="IV250_001531"
     CDS             10111..10986
                     /locus_tag="IV250_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP8768821.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(11138..11980)
                     /locus_tag="IV250_001532"
     CDS             complement(11138..11980)
                     /locus_tag="IV250_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8768822.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(12072..13949)
                     /locus_tag="IV250_001533"
     CDS             complement(12072..13949)
                     /locus_tag="IV250_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP8768823.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(13946..15367)
                     /locus_tag="IV250_001534"
     CDS             complement(13946..15367)
                     /locus_tag="IV250_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP8768824.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(15370..18759)
                     /locus_tag="IV250_001535"
     CDS             complement(15370..18759)
                     /locus_tag="IV250_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAP8768825.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            19016..20407
                     /locus_tag="IV250_001536"
     CDS             19016..20407
                     /locus_tag="IV250_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8768826.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYKLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(20480..21127)
                     /locus_tag="IV250_001537"
     CDS             complement(20480..21127)
                     /locus_tag="IV250_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8768827.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(21135..21821)
                     /locus_tag="IV250_001538"
     CDS             complement(21135..21821)
                     /locus_tag="IV250_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8768828.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(21827..22900)
                     /locus_tag="IV250_001539"
     CDS             complement(21827..22900)
                     /locus_tag="IV250_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP8768829.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            23122..23796
                     /locus_tag="IV250_001540"
     CDS             23122..23796
                     /locus_tag="IV250_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8768830.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            23801..25141
                     /locus_tag="IV250_001541"
     CDS             23801..25141
                     /locus_tag="IV250_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8768831.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(25290..26276)
                     /locus_tag="IV250_001542"
     CDS             complement(25290..26276)
                     /locus_tag="IV250_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HAP8768832.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            27000..28031
                     /locus_tag="IV250_001543"
     CDS             27000..28031
                     /locus_tag="IV250_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8768833.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            28140..>28564
                     /locus_tag="IV250_001544"
     CDS             28140..>28564
                     /locus_tag="IV250_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAP8768834.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIP"
BASE COUNT         8459 a         5337 c         5181 g         9587 t
ORIGIN      
        1 ctgaaaataa atccaaaaaa attcgctgca acgatataat aagacagcaa tcgcacctaa
       61 aacaattttc cttcatatgc tttagtgaaa gattttcttc ttaatgttta tttctccatt
      121 cattgggagt acatgttgaa tacttcttga ataatttgaa taaagaagtc gtttgtttat
      181 atccgatacg attagcgatt tcttccaacg aaaaactcgt ttcttgaatc agttccaacg
      241 caaagtcgaa ttttttcttt agaaccagtt cttgaaagga agcatcaaaa tacattttca
      301 atttattact aagatagacc ggattgtagc caaaatgagc tgctgtttct tttaacgtac
      361 aagaagtaaa atgcttatcg atataatcta atactctaag catctcattt tctgcgcatc
      421 catctgctga tgaaaagctt tgttgttcta ataaatgagt caattcgatc agtaatagtg
      481 atgtatagag atttaacgaa cggatcttat gactgtatgt atcaaaataa atcaacagca
      541 tattttcaat caaatgcgtc aaacgacttg tttttccgcc gcgaaaatga atgaattgtg
      601 tatgttcccc tttttctttt gatgcattta tcaaaaaaga agaaagtaga ttatttgtgc
      661 ttgccaaatg attcaatatc tctgtagata aggtttcttc ttttaataag atgttgatta
      721 aaatatcctc ttgccctgtg tattcgattc tttgagtagc ttctttatca atgatcacca
      781 cgtcactttc ttccatctct atcctttctc cattaataat ttgtaaacat gtaccgctat
      841 acatataatt gatttcaaca aaatcatgtg tatgctcggg aacttccgca tatcgacggt
      901 gtttactgat atgaatattt ccctgttgaa atagatcccc tcggtggatt tttggcggtg
      961 tctgaagatg gtaagctaag tttaaatcat tgatattcat tccttgttca cgctgcagag
     1021 tttcgattga ttcttcgtta cgcaaaaaaa atgtctaatt cagctagttc caattgactc
     1081 cctccttttt gtaagcggtt tctttcgctt attagttcac gctgtactgc tattatatgc
     1141 aaagctattt ttggtttcat aaagactttc tatggttggg acatgaactt cctgtgattc
     1201 cgctattgag agaataacca cttcatacta tatttttgat aagcgcttac aaaaatgaaa
     1261 ggaacatgct tatgaataaa aagatgatga aattttctct gctgtcagtc tctcttattt
     1321 tgacttctgc tggtgcagtc acgtctgcaa ttcccagtat gtcagctact tatgccacgc
     1381 gttctttatc tgctgttgaa ttattaacga ctgctccctc ttttatggtc actatttttg
     1441 ttttgattag tagttttatt gctaggaaaa tcggacaaaa acaaactgtg atcatcggat
     1501 tagtgattgc atcgatttgt ggtatcctcc ccctcttctc acaaagttac agtattgtct
     1561 tagcttcaag agctggacta ggtattggct ttggattgat caattctttg gcagtcagca
     1621 tgattgctgc cttttatcac ggagatgaac gagcctctat gattggtttt caaagtgcat
     1681 ttcaaggatt tggtgcagct ttaatgacat ttgttgcagg acagctagta cagttcggtt
     1741 ggcaatatac ctttttagtc tacgccatca gcttacctat tcttgtcttg tttgttttgt
     1801 ttgttcctac acctgaaaca actgaacttc aatcgcatgg cacagcccca acaagacaag
     1861 taatcaaaac gaatcctaaa atgattagtt atggtatttt tctcttgctg gtcatcgtgg
     1921 tatataatgc tgtatcgatt aaattagcca cagtcctgac gacttattct ctaggaaacg
     1981 aaactaatgc agcgaatatc ttaacaatca tgcaattggc aagtatggct gccggagttt
     2041 gttttggctg gcttcagaca aaagcacatc gctatatgct tacactttct ttactactga
     2101 tgggaatcgg ctttgtattg attgccactg tgataaacct gtatgtgatt gggtttggtg
     2161 cgatcctaac aggaatttct ttttcgttat tcattcctta cttattcaac caagtaagta
     2221 tccaagcgcc caagtacgcg gagagtttca atacttcaat cttattggtt ggcgctaatt
     2281 taggtacttt cattgcacca tatggtttgc tctttctctc ttttgcttct cttgggagta
     2341 aaacacttgc tgttttcgtc aatggcagta tcttgctgtt gattagcgcg cttattagcc
     2401 ttctagtagt acacaggacg aatagaaaga cagtgataag agtagatgaa gaaataaaag
     2461 aacaattgac aacaaaataa atttgatgga ggtcgtgtaa aatgacaaat gaaaaacata
     2521 aagatagatg gctttggtat cctggagact ttgagatccg ccatggactg ctacaaaatt
     2581 tccaacggga agaacgaggt tttgactggc cagcgtactg gtacatggat gattgccatc
     2641 ggaacgtcaa gtttaagaga tattactttc ttgatcaacc ttccatgttt aaagtgacga
     2701 tccaaggagt tgggtatgta gaaattaatg gacaaaaaca tccatgtgga aaatggctga
     2761 cttgtccagc tggtaaagca aaaattcgta tctttgtagg acatacatca gggctaccag
     2821 cgatgtttat cgagggagac gaagtaaaat cagatattgg ctggacagca agtaatttta
     2881 ttgaagaata tccagctggc tggagtccgc tttatgtcga tatcgcaaaa gatccaaatc
     2941 aaatctatta tcaaaaagaa tgcgtccatc cagtcactga aacggagatt ggcggaggaa
     3001 tcttattcga ttttgggcga gcagtgaatg gctgtgtaga actaatatct ctggatgacc
     3061 atatcgaatc tatcactcta tgttatggag agtccgatgt agaagctatg gatatcgatt
     3121 attgctacta taagcaaata ggaatgacaa gaaaaatgaa agctcgtaaa cgcgcttttc
     3181 gctatttgtt tataccagag gtttctactg gaatggttca acttcaagtt tttcatgaat
     3241 ttttatctct ttcgaaacaa gctgcttttt caagcgattc ttctttactg aatcagatct
     3301 ggtcagtatc agaagaagcc ttcaagttat gtagtgattt gttttttatc gacggtatca
     3361 aacgagatcg ctggatttgg tctggagatg cgtatcaatc attcatgatc aatcaatatc
     3421 ttttttttga cgaggaaatc aataaacgaa cgatattagc attaagagga caaaatgaaa
     3481 tcaagcagca tatgaatact atcgttgact attcgatctt gtggttgatt ggtatcgaaa
     3541 accactatat gatgacgaaa gactatgaat ttttacgcat cgtctaccct aagatgcaat
     3601 cattaatgag atacttgatg gagcaaacga atgaattagg gtttatttat gggagagcaa
     3661 aagattggat atttattgat tggtctgaaa tggataaaga aggaacgctt gctgcagaac
     3721 aagtcttatt actgaaagcc tatcattcca tgatcacttg cggagatatc ttagctgaaa
     3781 catatggaga gaacgaaaat tatcatactc atctctatca agaaaaatat caactattga
     3841 aacaaaacct atttgactac ttttgggatg aagaacttgg cgcttttatc gattgctatg
     3901 aatctgggag aagaaatgtg acgcgtcatg cgaatatttt cgctgtttta ttcgattttg
     3961 ctaacaaaga ccaacagcaa tcgatcttaa agaatgtctt attgaatgac actatcacac
     4021 agattactac tccatatttc aaattctatg aacaagatgc tttatgcaaa ctcggacaga
     4081 cgaatatcgt ctaccaaacg attcttgatt attggggagg gatgttatct catgatgcgg
     4141 taactttctg ggaagaatac gaccctactc aaacaggaaa tgaaaagtac agtatgtatg
     4201 gagatcctta tggaaaaagc ttatgccatg catggggagc aagcccaatc tatcttttag
     4261 gacgctattt tatcggtttg tatccaacaa agccaggtta tgaagaattt atgatacaac
     4321 ctaatctaga ttcatttgaa acactgaatt gtacgctacc tatcaaagat ggaaagattt
     4381 cgcttgaaaa aacaaaagat aagatcgtga ttacttctga tagaacaggc ggaacagtga
     4441 aaatgaatgg taaaagttat cctcttctag caaaacagcc acttgtctta acaagctatt
     4501 caaaaataaa tgcataaaac aaaaaactgc ttgcgacata tcagaagact ttaaatcttc
     4561 ttctgatatt gtccaagcaa tttttatttt gaagtttgct taattctgtt ctttagaaac
     4621 aatgtttgtt ctttttccgg caaaaagaaa gctagataat acgacaagta aaaagaaagc
     4681 gatgattcca actgtcaagg ctatttgacc gtattctcct agtttcccaa gtacaagtcc
     4741 tggtactaga taatcagtat cggcaaatgt agaagaagcg attttaagat ctcctaatac
     4801 tggaaggata aataatggca gccaactaat caataagcta ttgaaagcag aacctaatac
     4861 cgcccctctt ctaccgccag tcgagttgcc gaagacacca gaagcagctc cgcagaagaa
     4921 atgtcctact accccaggaa tgataactgt agtttgcaag cctatcatag caaacatgga
     4981 tagtgttcct acaaagaagc taacaaaaaa gccaattaat acagcattag gtgcatatgg
     5041 aaaaacgata ggaacatcta atgctggttt agaatcgggt acgagttttt tagcaatccc
     5101 ttggaatgct ggaacgattt ctgccaacac cattcgaacc ccttgaagga cgataacaaa
     5161 cccagctgtg aatgttccag cttggatcag tgcaaagatt acagcgttgt ctccattgct
     5221 tagttctctt tcaacaaatc caggaccagc taacaaagca agtgcgacat atacgatcga
     5281 catcaagagc atgatcgata cagtagaatc tcgcaaaaag ctgaaacttt taggaatctt
     5341 tatgtcctct gtagaacggt ctttgtttcc aaaaaacttt ccgatccatc cactagaagc
     5401 ataaccgata tttccggtat gccctaacgc aacttgattg tttccagtga tgttcttcat
     5461 aaacggttga caaagagcag gcgtaatagt taatagtgtt ccttcgaaga ccccgcctaa
     5521 taagatcatc cacaaatttg cagtaaaccc cgcgcttact aaaatgactg ctgttagaca
     5581 agaaacataa agcattgctt gacctgtaag aaaaatgaat ttatatcttg tgaatctagc
     5641 aagtaaaata ttcacgatca tccctactag catgatcaaa gcagtaggtg taccgtattg
     5701 ttgcaaagca atcgccacta ctgcttcatt gctaggaact actccttgag tatgcagagc
     5761 tgtttggaac atcgaagcaa aaggggttag tgaattactg attaaaccag caccgccgat
     5821 cagtactagg aatccgacaa atgttttgat agttccttgg atcacttctg aaaatttctt
     5881 cttctgaaga acgagaccaa gcagtgcaat caaagaaacg aggatggagg gctgactgac
     5941 gattgacaca aacaaattca acaatttatc cattgatagt tcctcccttg aatcattttt
     6001 attcttttga caagatcttc gttacttttt cttttacttc ttcgctgtca atgatcgatt
     6061 gcaaaggtac gagcttttca tcaggtatag aagttagcgt atctttcaaa gaatgctcaa
     6121 tgaagaaata atcagccata tcgctagata cacttccaac atccgtatga cttacttcaa
     6181 actcgtccac atctgctcca agatctgcaa gtgcttgttt gatgttcatc tctaccataa
     6241 aacttgttcc taatccactt tgacatactg caaaaatttt catatcattc gtctcctttt
     6301 cttatgatcg ctaatatttc ttcttttgtt gatgcgtgta ataaatcttg aagattttgt
     6361 ttattagaaa gtagctttgt taaatttgcc aaagcccgaa gatgtgcctc attatcgata
     6421 gcagctaaac agatgaacaa actaatggga tgggctggat tatcaagcag atccacaggt
     6481 tgttgaagtt tcagcaaaga catccctact tcgtttacgc catcttctgg acgagcatga
     6541 ggtaatgcga tgtgatcgcc aatatggata aacgcaccat actgctccac gcgctcgatc
     6601 atagctttga tataactttc ttctatttta ttttctgata atagcggctg tgcggcaaat
     6661 gtgattgctt cttgccagtt ttttggttga tccgataatt ggatcatttc ttctgttaat
     6721 aattcagata gcataggacg ttgatcctcc ttcttttcta aaagtttgtt cattttacga
     6781 tttaatactt gatagagctt ttcttcgtct acgccttctt ttaattcgat ataaggcagt
     6841 agcgccgaca acaattcttg ggcagaaggc attgaaaatc ctggtatcag ccaatctctt
     6901 tgtacttgat tgatcagctg atttttttcc aagtcagtca tgattggatg cagaacatag
     6961 agagactttt tcttatcagt aagtgtgaga ggtacagtag aaaaaataat atcgtaagaa
     7021 gtttcgtcaa cctcgtgtaa gcggtcagct gaattagtct ctgtaaaaaa gatcatcggg
     7081 aataattgct gaagttcact ttttaagatc aatgaagagc taatgccact tggacagacg
     7141 atcacggctc ttatttttct atttctttct tctgcgtccg cttttctgat ctctccgcca
     7201 aatagtatag tgaaaaaacc aatctcttct tcagggatgc tcttccctat tcgtctttct
     7261 aacggaagta atgcttttct tgtcatttcg tagatggaag catattgatg cttaatttga
     7321 tcgatcatca cattaggtaa ataaaaagaa taggaaatac gaaaataggc agggaccaag
     7381 tgataaaaga cattcatcaa taattcacga aacgaagcaa attctacggc catcaggaat
     7441 tccatgcgat ggatgacttc tgaagaacat tccagcaaaa aatcaagtgc aggctcctgg
     7501 acatctcctt caacgatcga tgctaagcaa agagagaaaa atataatctc cgaattttga
     7561 tgttcggaat agccaaggcc agtcaatact tgtcgactaa tgtcagtgac tgcaagagaa
     7621 gccaacagtt cagcatcttt taccagttcc atggatgttt ttccaattcg aatggttgtc
     7681 aaactcaaat aataagctaa ttcatccatt cgatttggtg cgaatcgaag atctgatagt
     7741 gaaatcgttt tatctagcaa atctctaact ttggcgtaat aatcaatgga aagggttgcg
     7801 gttaaatacg tgagcccaaa tcttccagca agtgactgca tcaaacgagc agtgcgattt
     7861 tttgcttctc ttcttaatat tgcttctgac cctagcagcc taaaaccagt tttcctagta
     7921 tacgacagct gacaatcttc cacttctaat tcatttctta tctttttgat atctgacagg
     7981 actgtccctt tagaaacctg aagaaaatct tgaaaatgaa aaacagaaac agacgttgca
     8041 gtcgaataaa ccatcaaata aagaagatct cttcgctctt gttcggtata cacagcttca
     8101 aatcgagtaa tatccaaaaa tttttctgcc cagtcgattg ctcggaattt ctttatacgg
     8161 atacgggtat catccacata ttcggcaaaa ggagttaatt gtttgatcgc ctcgcttgta
     8221 tttacttcga tgatctctgc tgctttttct agactgattt ctcttggtgt tttcaggaga
     8281 taactaattt tgtagttcac agcgctgttc acctcctact tatattctag tatggtagcg
     8341 cttttaaatc gaggggtgtt tggtacaact tacagttgga ttttttttac gactgtaccg
     8401 aacgatttct atcgaatgga tacagaaaaa aagcaggtga ccaaatgtct cctgctttta
     8461 tgaattgatt tcgtccgatc aatgatgatc atgtttattt tttgaggtca gttcttcaaa
     8521 tttttctttg acatcttcaa ccgctttttc gatatctttt tcaaagtgat tgggctcatt
     8581 aggctttgtt tcctcttcta cagaatttga accagataaa gctgtttctt tattttctgt
     8641 ttcaccagag tttgtgccag ctaatgattc ttctttgtct tctgtttcac cagaattagt
     8701 tcctgctaaa gccacttctt cttctgtttt aggggccatt gaacctctta atcgtttgtg
     8761 tttttcaccg tcataatttg tcatgttggt tcctcctagt agttctatac tttttcatcg
     8821 tacaataaga cagtttatgc gtcaaaaaac aaacccgatt cttacaaata tgtcatattt
     8881 ttccttctga ttcttgaatg cttttgattc cttcttccaa aattttcatt tgtaagattg
     8941 tttcttcatc tttgatgact ttttctgcgc cattaactaa tgtctcgtaa atcccttcgt
     9001 agacacggct gtaatcacca acctcggaaa ttactttttc ttcatgataa ctcccttgtt
     9061 catctatata ggtcaataca ccataatgtt cgggcaaatc gataccaaag tcttcatgat
     9121 caggcatgta aaacattttc agatgttctt cttgtcgatc ttttgtttgt ttcacgaaca
     9181 tccctttttt tccgtaaacg acgaaacttg gacgttcttt gatgcggaaa taactggatt
     9241 ttatcgaaat tttcatattt ccatagaaaa aatccagatc gaagtaatca ttcattcgtc
     9301 ctttccctaa aagttgacgt acatcatagc tgatatcatc tggttcgccg aaataagaaa
     9361 gaacttgatc tactgtatga caagcatgtc catataaata gctgttcgca gtagaaaatt
     9421 catgcgtatt ttctggtact tccggtctga aataatcata atgcatttct acttctaaaa
     9481 tatcccctaa ttttccgctt tcgattactt tttggactgt caagaagtca gaatcgaacc
     9541 gtctgttttg gtaacattgg ataaatagac ctttttcttt tgctaatgca aataactctt
     9601 ctgcttcctt tgacgtttct gcaaaaggtt tctctactag tacattctta ccatgttcga
     9661 gtgccatttt tccatattca taatgaaatt gactaggtgt agtgatgaca actaattgga
     9721 tctcaggatc atcccaaaga tcagacagct gatcagtata ttcgatgtct tcgaatgttt
     9781 tccacactgg ttcagatggg gaaaaaattt ttttgatatt gattttgttc gtaagttttc
     9841 ttgaaaaagg caaatgatag cgatttgtac tctttccatt gccaagataa gcgattgtaa
     9901 gcatgagtgg tgcctccttt tattgatgtt ttcagtatat cctagaaaag cgcagaaagt
     9961 atttataata gctattctgg taaaaaagtt cagaaaaaca cttgttatca ttcggtttgg
    10021 tgaaattaat gttcaaagta aggataaaaa tcaaaaagat agtagcggaa ccaagtgttt
    10081 atcagtaaga tagaatttgg aggtcagatc atgcttattg aagaaaaact aacaaaacaa
    10141 gagttgttta ccacgacaga gaaacggatt gtcgattaca tccgtaggaa tatcgaagct
    10201 gcagtctaca tgacgattga agaattagca aaagctacgt atacttccca ttcagctatc
    10261 attcgtttat gcaaaaaaat gggattcagc ggatttaaag aattccgttt cgagctgggg
    10321 cgggaagttc atcaattgat tgctcagttc aatcaaacga ttgatcctaa ttttcctttt
    10381 tcaagtgaag atagcactca gcaaattgca aagaaaatgg cggatttatc gatccagtca
    10441 ataaaaaaag cacaattgca gattgaacat gaagatttag atcaaatcgg taaagtattg
    10501 aacaaggcac agcgaatctt tctttttgca aaaggtgatt ctcaaatcac ggcaaggaaa
    10561 ttccagaata aaatggtcaa gttgaataag tttttgatca tggctgaaga atacagtgat
    10621 tcttcttgga atgcagccaa tttgactagc gaggattgcg ccatttttat tagctacagc
    10681 ggtcgcatcc atcattatga acgaataatg acctatttga agcatgtaga ggcccctacc
    10741 ttgctgatta ccggaaatca gcattcagaa atggccaaac aggctgatat gtgtcttgtt
    10801 atatcgcagg aggaatatga ttttgctaaa gtagcgactt tctcctctca aattgccttc
    10861 gattacgtat taaatacgtt attctcagtg atatattctc aaaattttga agagaatatc
    10921 cgcaacttga aaaacaaaca agaattgatg caaaaaggcc ttcttaaaga atatcggaaa
    10981 acttaagaaa agatcctcat ttagtaataa gaaagatagt gtttgatagg ctctctttga
    11041 tatcatcata aaaacaggct gaagttttct ttttcaaacc tcagcctgtt tgtttacgga
    11101 acgattcgaa tagatatatt atagaaatga ggaatagcta acttctgttt cttattttga
    11161 acgattgacg aaaaagatat ataagaagga ggaaaatcag tatacatgca gctaacaatc
    11221 catacaggaa aaatcgtttg ttctcggata ctttctgtac ttctttttga acttcttttg
    11281 catgataagg aacccggtgc cctctgacaa gtaagcgatg actgttgacc atataaggtg
    11341 tacaagttag gagtgtcacg aaatcctgcc ctttttcgat tctcagatct tttgtatcag
    11401 tcggttctac cgtcttgatc tgatcgactt gataagcgag tgtccgtcca ttgatttcga
    11461 tatagaaaga atcacctaaa gtcagtttcg gtaagtctgt gaacaacttt gcttgaggca
    11521 gtcctcgatg gctggaaata acggcatgtg tattttctcc ccccgtagga taagacgttc
    11581 cttccaataa tgaggcacct ttttctagca gcacagtatt cgtttcatca aaaattggca
    11641 aatggacatt gatctcaggg atcgtcaaaa taccgatcgt atgtttctca aaataggttc
    11701 tatccttttt cgcaggttct tttttcttag taaagggatc tgcacctgga tttccacctt
    11761 cttttgctaa ttgctggttt ttctttgtca tgttttcttg gattttagcc atgacttctt
    11821 cattttcttt gaccgcttgt tgctgataat gagagatgat ctgctgatcc agataattat
    11881 tcagcgcgtc gctaacaaat ggataagcta gtatccctat tcctgagaga agcaagaaaa
    11941 gcatcagcag atctagaagt ttccttttct ttggtttcat ttgtgttctc cttgtgatga
    12001 ctgtttgata ataaaagaaa aaggagcatt tgcgtaatcc gctgctcctt tttccaattc
    12061 ttgcaaaaag actaaatctg actgtgcttg cgtctagcaa agtacagtcc agcaatcagt
    12121 agaaggacta ctcctgcacc gatatacaca tagattccct taccgcctgt tgaaggaagt
    12181 gtacctttgt gtttatttgg tattttttca ggagaaatca actgtcctgc tgttacataa
    12241 gattgttcat cgatagtaaa tgctacacgg tttgttaatg gaacatattt ttctggcgct
    12301 ttcgtttctt ccagatagta cgtgccatat tttagacctg tcacatcgat taaaccgtta
    12361 cttgtggttg taaaaaccgt tgctgattct ttcgccgata cccagctgac ggcttttgtg
    12421 gatgggtcga tcgataaata tttcgctgtg tcactatctt gatcacgaac gacgaattct
    12481 gctccagcaa gtgtttggtc tgatgtaacg tcaccatcta ctttaacaaa tcgtttgccc
    12541 ccagtaacga catcgactga cggtggtgtt tgatcatttg tatgcccgtt atcgacattt
    12601 gcttggttag aaaatccttt ggttggatct gctttttcgt tcaaatgcat atagtaaacg
    12661 aatttcaatg taccgccagg agttaatgaa ggaatataat tcggatcaac tgaaaccgtg
    12721 aatccgtctg tttgctcagt gacagaataa tttactgggt cgatttcttt atttccatca
    12781 tataaggcat aagcatacgt tcctgaagaa tcattatcaa atgttaaagc agcgtcatga
    12841 gtatcgataa gtttgaatgt tgtgtacttg ttttgcgttc cttctttatc agcgatacct
    12901 aatgggatat tgacagaaat ctcatattga attttttcac cgattgcgac atcttttcca
    12961 ttcaattgag gtgttgtttt atcaacttta gacgtatcat ttttgatggt cttttctact
    13021 ggtgtttggc tatttgccag gatctcaaaa ggcgtgattg tttgcttttc aatcatttcc
    13081 gcattatcag gagcttttac ttcttctaaa ttatattttc caacttctaa atgattaacg
    13141 atcaactggc ctttttcaat agatgcctcg gcaaagtcat tgttgccgat gtcataagaa
    13201 tgaccagtaa tgaaatgttt ggctttggtt tgatcagttg tccaagtgta caatccatct
    13261 gtgacacttt ggatgtattt tttgacgctt ggtgttcctt cttctttaga aataataaat
    13321 tctgctccat ttagtgcttc gttttcggca gtaccgattt ttgtaacttt caacgttcca
    13381 tcattaccga ctgtattttt agggtagaga tggatagtat ctagttcttc tgtcccgtat
    13441 ttataagagc catctgcttg tttgatcatc tcatatacag ggaaagctaa aaccatgttt
    13501 gcggcagctg acactccgtc ttttggttct tctttgatcg tgtagactgc atctttccca
    13561 ttttgttttt taggtaaaga taaagtgaca tttccatcag catctgtcgt tcctgaagca
    13621 actggtgtac caggagtcaa agactggact gcttgttttg ctgcatccac ggacgctcct
    13681 ttatctcgtt gcgcataaaa ttcttgagtg acgttataaa ctgaaaatga aatatcggct
    13741 aatccttggt attgatcgaa ttcgctcatt tctttcccgc tgttttggat taaaggatcg
    13801 ggtaaatcag tcattttctt tttatgaagg atgacttgag ctgctgtctc ctctgcaaac
    13861 actttttttg cgccgaagct gtttgtgagt aatggtaaaa taaggaaaag gactcctaac
    13921 ataacgttta tttttttatg atttttcatt ttttcactcc tgttcttttt ttgataaaca
    13981 accagattgc aaagcttgcg aataccatac caacaacgcc taaaagtgta tttttcgttt
    14041 cgtttgtttt tggaagaaat ggacggctat tttctttttt attgactact ttcaatatac
    14101 tgtccgcctc tttatcctca gaaagaaaag ttgaaatggt aaacggaatt tctgtttcac
    14161 ttaaaatata accagaaggt gcttcgatct cttttaatcg ataacttcct gttttcaacc
    14221 cggaaattga aaaagcgcca tttttatcgg aaatcaggtg aagcgcatct gattcgttct
    14281 ttgtccactt gtatccgttg gctgtttgct ggaggtaatt tcctgctttg tttttaataa
    14341 ggaatactgc gccagctaat cgtttatctg gtttatttgt ctcgattttg acaaattgct
    14401 tactccctgt atatacagag actttctttg tactgacacc aaaaccaaaa tccagtgttc
    14461 cgttgttgtt gatttccttg ttcgcctgtg ctgtactgct tagctgcatc tgataagaaa
    14521 tggtcagttt ttttccagca tatttttcca acgaagggat cgaaaataaa acatcaaatc
    14581 cgtgaggttt cttgtgtatg acaaagcctt ctgtcagctt ttctccattc gatgaaatcg
    14641 ttaaactatt aggtaaaaac gttaatgctt catccgcact atctgacaat tcgaactttt
    14701 gatagtcaag gatatttaca ggaattgtcg tatgcaagga ataagtgatt ttttcaccaa
    14761 tcgtaaaatc atttctaggc tcttctaata ccttctcaaa aggtggatca gggtagtcgt
    14821 tttcttcatt tttaggatac aaatggatag ttgaaagttt ttgattgttt tgtccatgta
    14881 caggaagaac aactaccata ttctctgcct tttctttgac gacttctggt actttggatt
    14941 caatgaaaag atacgcttta tctctttttt cttgatcttt agcggccaga gaaaaagaag
    15001 cgataccatc ttcgttgtta agagttgtag ttgttgcttc tgcggtaaac ataccggaag
    15061 acgcaccgtt ttttgcgatc tctgcttgtg cttcttctac cgttttgccc ttttcccgta
    15121 gatggtaaaa agaatctgtg acatcataaa cttggaatgt gacaccattt aatcctcgat
    15181 acgtttgtaa taaagctttt tcttgtccgt catttggatg atttttcggc agttgcccgt
    15241 ttgggaaaag caatttgtgt aaagtaatct gaaccatttc tgtttctgtt tctgttgcta
    15301 cctgagtaaa agctggttta aatagtaaca agaagagaca catactaagc caaccaagtt
    15361 ttttcatatt tagctgcccc ctctttcttt tttcaaaaaa agataccaaa tagaaaacgc
    15421 acaaccaatc atccctacta gatagattcc taaacggcca attcctcccg tttcaggtaa
    15481 tggtactttt gcctgattcg tgatgtctaa agaaatctgg ttgtttttgg ctcctggtga
    15541 cagaacagat tcatgatctt gtccatccac ttgaattgac ccatcttcgt gtataactat
    15601 agtaactggc tcttttagac cttggtatcc ttctggtgta aaagtttcgg tcagcgtata
    15661 agttccaggt tttaaatttt cgaatagaaa ggtatctgtt tcttgtccat cttttggcga
    15721 ttctaagtcc atttctggtc cctgcagtct gaattttgct ccttttaatg tctgtccgtt
    15781 gtcttctttt ttgtgtaccg ttagatcaaa cgcttttagt cgattttgat gagtgagcgt
    15841 ccatacatta tttgtcatct cggtattttt tcccgtgtaa atgatttttc catatttatc
    15901 gatttggaat tcataatttc ccggcgaagt atcatatccg gcaggaccag ctgtttcaac
    15961 gactcgatat ttaccaggtg tgagtgaatc aaaactggca agaccttttt cattcgttgt
    16021 ttggctgtca attggctgat aagtaccatt tgcttctttt ttctgtaggg aaaatgctgc
    16081 tcctttaaga tttatctctt tgtccgtccc ttgcatcgca tacttacgta ctgctacagg
    16141 aacttcaaca aaaggatttt caatagtcaa ctggatcgta tcatcggaag tagtgactgt
    16201 ttttccatca atggttaccg taccatcaga agcgatcttg atttcccaag tagtcttttt
    16261 gcttaatcca tgcccttctg gagcttttgt ttctgtcagc gtataggtca tttctttttg
    16321 caatttgaca ttttctggaa gagaataggt tccgtcgcca tgatctgtta gtaaagtatc
    16381 aatagaatct cctgttaatt tgaaaacagc gccaataaga tccttttcac ctgtagagga
    16441 atttttcgtt attttcaagt ttaacggtgt gaattgttta gtatttttcc atgtcattgc
    16501 atctatttgt tgagaatcgt atcctggtac aggtaattct ttgattgttt ggtaactgaa
    16561 tgcttgacct tgattattgt attgaggtag tgataatatc tcttgataac tttctccgct
    16621 atttgcagat aatttgtcaa tgtctgcacg ttcccacgta tttgttgtat ctttagctgg
    16681 tttaatgatt cgaatatatc cgtttttcca agctgcagca tttgtcgtat gttcccgttg
    16741 aatctcaaaa gtaacttgat ctggacgatc agctagattg ttgtcaaatt cttcccataa
    16801 ctttttgatg tgaagactga ctccgggagc tttagcagat ggtataccga attcagctaa
    16861 ttcattggta tcaatgtttg gctggaaagt tgtcctgccg ttcatttgat accagaaatt
    16921 tggatggaag tcctcatttt ctgtttggat tctcacttgg taatggattt ggatttcttg
    16981 gtcttttcct aaatagatct gattgctctt gatggtattt ccttctatgg aaatagatgg
    17041 agtgaccgtt gtaggacttg tccccacact cttgactgaa agtgtaccag gctgataaat
    17101 gaatggttct gcgattggat catttatttg tccattgctt acagttgctg agatctgtac
    17161 agcttttttg gctaggtatt cagagatatc tgttgcatga tcagctgatt catagtaaag
    17221 atcgcctttt tcatctgatg aaaccatttg tcgcatacga gactctactt ctgcttttga
    17281 gagaccagct gccggatcgc tttgaagttg gatgccaaga ccatgtattt cgattcctcg
    17341 ttctttgagt gccatcgctt ctccgatggt tgcgataaac gtactgtcga ttcttctgga
    17401 tagattgttt tggtcaggtg catcatagat tcttgagatt agagaagtat ttcccggccg
    17461 atcttgcgta ttagaaaact gagttccgta ataattgctg cttgattgtg cgtgtacccg
    17521 ctgtacttta taggaaaatg ttggtacacc atccgtcagc aaaacgatca cttttttatg
    17581 tccatttgga acggatagca tgcttcctgc atctcttagt gctttttgtg taaaagtacc
    17641 accatttgtc cgtgaaggtg taatggattt tacttgattt ttcactgaat caaatgaacc
    17701 catctgtact gcaccgttac tgtagctata tccttcgctt gagtagccga catatcccat
    17761 attgattttg tctgtgatac cgctatctgc taaagtatcg acaaaacgat cgacaccaat
    17821 ctttacttca ccgatccgat tattgtcgtt catacttcct gaccagtcta cgaccaatac
    17881 gagatcaaga ggcgtgatat ctttttgtac atttccacga gcattcaaat aaacatcaaa
    17941 caatccgggt gtacttgttt cctttgcata ctttcgtatc gcatagtctg cttgattacc
    18001 ggttcctcca tattcgatat acgaatgggt ccgatcatta ggatctccat tccaagaatt
    18061 tataccatcc cattgatttg ttccgttttt attgccttga tgatcaagaa catttgtatt
    18121 tcctgtaggt tgccaactgg ctttaggata agtgccctga tcatctgttg tatattctgt
    18181 aggaatcgtt tgattagatg gcacactcac agctggcaag gataatgtag gcaatgtaaa
    18241 tgggctgaca gaattttcgt tttcttttga tgattcttct tctatttcgt ctggctttgg
    18301 tactgacaca tctgtatctt tcttattttc tgtctcatca ataatgtcat attctttatt
    18361 atcgacgatc agcttgtttg cctggctgtg gtaaagtgct ggatcgattg gaaaactcag
    18421 atcgatagtt tgtttagtgt ttgatgagat ttccaacgta attttcccgc tatttacctg
    18481 ataagcatag ccatctcctt cttttctttc cacatcactt aagtaatttt cgactagacc
    18541 aatttctttt tctatctttt cagcactggt gttattcgca tttagcttga tgttcagtgc
    18601 tttttttcca gactgatccg aaatgacttc agctgaaatt gtttctaaaa ctgttggatc
    18661 atctgtagtg tctgctattg cagataatgg cacaaagttt gataaactca atattatcag
    18721 catgaaaata actttcagtt tcttccctgt ggtcgtcatt tttcccctcc tttttctttt
    18781 tttctgtttt gacgtgaata gtgaaaatta ttaaaataaa caggcgttac ataattttat
    18841 ttattttaat atgataatta ttatacgaag ggcgctaaag ggaatcacac caattttcat
    18901 accaattggt ttcatttatg aagttgattc cttcgattaa tgagcgtttc attgaaagaa
    18961 aagggttgag ggagaaaaac atatgatcag gttgttggaa ggaaactata gaaagatgga
    19021 aaaaatcatt cgaattctgt ccatttctga caagtggatg actaaaaaag aacttgccaa
    19081 acgaattggt agttcagaat ctacatttgt aaggtatatc gaagagatca aaatccgctg
    19141 gggaacggat gtaacgattc aatcttcaca aaaattgggg tatcgtcttg aacatttgaa
    19201 tgtaccagct tatttaaatg ttctgatgga aatgtcgcaa agttctacaa acagtcgttt
    19261 attaaatgag attattgtca atccgggtaa aacgattgag tattattgtg aaaaaatttc
    19321 catcagccgg tcttctttta cacgtaagtt gaaacaatgc aatcaaattt tagaaagcta
    19381 tttattaaaa gtgatcgttg accaaggttt tcagttaact agccttaaaa gtgagctcca
    19441 gttgcgtatc tttgttacct ttttcttttt aagttactac ggaagacgca cgttgccatt
    19501 tccaatcgaa aaaaagaaga tcaaagaaat tatggtcaag aataattgtc agatgaattt
    19561 gtttcaggaa gaaagcccat atgagcaatc tttctttatt atgtattttg ttgtgcatct
    19621 catgagagaa tcccaaggaa ctccatttga atggaaagct ggagactctt atgtacagtt
    19681 gataaatatc gataaaaaag acttcttaat cttgaaaaat ctcttaggaa aattgaagta
    19741 cagcagctat ataaaagtag tgaactattt tgtcaatacg attttttcag aattttcgga
    19801 gaaagaaaaa aatgaaatca gtgagcagat acgaaaaaat atgtcctact gttcgttttc
    19861 atccgctgat tgcaaagaaa aaaacgaaga atatgttgtt caattattaa cgcaaattta
    19921 ttttttctcc cgtattttcc catacgatac gactcatttg acgatccgtg caacttactt
    19981 tgcacagcag atgcaacacc gccagatacg tctatatgct ggatttcgtg cagaacttca
    20041 attttattcc actctactga atcagaatct gatttattat ttatctagct atatcttttg
    20101 gatgcttatc ggtaatcctg aaattcacca ttatacttct gataaaaaaa tactgattat
    20161 cagcgactta agtcttcgac atagtcaata tattgaagaa tatatcagtg atattcttgc
    20221 tgtgcataaa attcactctg agacaactgc tattactgaa gatcagctgt cgaaatacaa
    20281 acttctcgag tatgatctga tcgttacgaa tcaacccatt ttgaatgccc atgttcctca
    20341 tatattgatt gatgatagtg tgtcattcgc aaatgaagaa gaactcatcc gcttattcga
    20401 gttatgaata atcaaaaaac agctatgtat aaaccaccct gataaaaatt tcaggacaaa
    20461 tacacatagc tgtttctttt tattgtattc cttcagcaat ccaacgagat ttatagcgaa
    20521 agtcttttaa caactcttct tttgtccaat tatttgcaat gagatccgtc aaagtgttca
    20581 ttacgagggt catggaaaag tataggaaac taactgcttc ttccgacgat tgaatggaga
    20641 aatgattgat tcttaataat tccagccatt tttcgttcat tggagaatca gccgatagtt
    20701 ctctacgttt ggttgtctgc tgatcatttc gctgggttaa caagtataac ctttgccaat
    20761 agatactttc gtgatctaag tcgcagcacc actctaaata tttttcgatc cccaaaaaga
    20821 aatgttgttt gttttcgcta atgaatttta agatatcttg atggactatg tgagaactgc
    20881 ttttgatcgt atacaaataa gcatcttcca aatcttgaaa atatttataa aaagcaccgc
    20941 gcgacatccc catcttgtca acgatttctg agactttgac ttggctgatg tgctgtgagc
    21001 aaaatatctc cagtaatagg tcatcgattc tctgtttctt tgcgttcgat aaattgaaaa
    21061 aagtttcttt tggcatccac atctctcctt tatctctgat tatacgtgga tagcccttca
    21121 ttgtcatgaa ggattcattc cttctttttc tcttcttctg tcaagtaccc atcttccatt
    21181 cgataaacgc gatcactcca ttgaatcata cggacatcat gagtaaccat aatagtcgct
    21241 ttttgtttct catgagcttc tttaactagt aatttgacga cttcatatgc atgatctgta
    21301 tccaaactag ctgttggttc gtctgctaag atcaagcttg gctcattata taaagcgcga
    21361 gcaattgcca ctctctgtct ttctccacca gaaaggtctt taggataact atttttaagt
    21421 gactggatat ctaaagattg cagcaaggaa tcgatttttg ttgaatcttc tttttttgaa
    21481 gtaacttggt caactagttc aaattgttcc tttaccgtta aaaaaggaat caaattagag
    21541 ctttggagaa taaatcctat ttctttaaag cgtaaatctg ctcgtttttt ttctggaagc
    21601 tcagttagtg agtgaccgtt gatcactatt tctccggaag tcggtgtttg caatcctcct
    21661 gaaatggtaa ggaaagtact tttacctgaa ccagaaggac caatgatgct gacgaattct
    21721 ccttccttta cataaaaatc aattgctttc aaagctgaga cctttgtatg accagaacca
    21781 aatgttttgg taactttttt catcgttaat atttttttca tttttattaa cctcctattg
    21841 ctgtaattgg gtctactttg gttatcgtac gtatcgaaaa caatccgcct agcattgcga
    21901 caatgatcaa aatcccacta tatagcaacc attgtgacca aaaaacagca aaaggcatcg
    21961 cgcttggtaa aatcagacta gtcaaataag caaacaaaag agcaagcaga acgcctacca
    22021 ccccaacaat aaaggactgt gctactaaag atttagcgat gaagaaattt ttgatccctt
    22081 gtgctttcat tactccaaaa atagcagtct tctgaagtgt catcacgtac atgaaaatac
    22141 cgaccacggc agcaaccact aagaacaaaa agtatatcat cgcatttaag gtcatatttt
    22201 gggcagaata gccaggaata ctttcaatga atgtcccaat agttaataac tgtagaccct
    22261 tcgatgcttt ttctttcgaa attttagctg aatcctttat tacgatccca ttgatcggct
    22321 gttcttcttc agaagcaaac ggctgttgtc catacttcaa agcagtccac gtatctaggg
    22381 acgcatagat gacgggagaa actgtgtaat aagtttcagg aaagataccg acaatcgtca
    22441 aaggatcgtc gtaactgcca attttgatcg tatctccaat tttatagcct gaatcagcca
    22501 gattttgaga gatgatgatt tcattctttg ttttaaattc atgccctttc gtgagttttg
    22561 gtaataaaaa tgcttctttc gttgtgccga aaactgtaac attttctttg ttttttcctt
    22621 tgattgctcc gctgtacaat ccgattggtg ctttctctcc tccttcaatg tatttcagat
    22681 ctcctcttgt taattgtgaa gctgcaaata cttgatttgc atcctccgac aaaacaatct
    22741 cttgtgcgtc ccaatcatcg attgcttttt taaactcttc tgaaagtcca ttcgctagtc
    22801 ctgacaacat gaatacgaca tacgcaatca agaacataat accgacgatc aagccatatc
    22861 gtaatttaga atagcgaatt tctttgatag ctagaaacat ttgtttttcc tccctatgat
    22921 agattgatag tgacaaaatg tcacttgttg aatagtgtaa ctctaaagtg acaatttgtc
    22981 acttgtgttg tttttttctt atcaataaat tgtctaagac atctttcgat aaaaccttaa
    23041 catgagtcat aaaaaattta tttaagcctt atttatgctt tccatgataa actaaagtga
    23101 ccaaatcaat gaggtgggaa aatgattaaa ctattagtag tagaagacga taaagtatta
    23161 tccaataata taaaagaaat actcgctgca atcggcgaga taacacaagt ttatgatggt
    23221 agtgaaggac tgttcgagtt ggaaagtgga gtttatgatc ttgcaatctt ggatttgatg
    23281 atgccggaaa tgaatggata tgaagtgtta gcaaaaatac ggaaagaaaa aatacagact
    23341 ccagtattga ttttgactgc aaaagatagt ttggaagata agattgaagg atttgaacga
    23401 ggtgcagatg attatctaac aaaacctttc tatcgagaag aactgatcat gcgcgtgaaa
    23461 gcattattga aacgctcgct aggtctcttt gatgaaaatg ttctgaagta taaggatatc
    23521 agttgtgatt taaaaagcaa tgaagtcttg tataaagaac aagttttgcc catacaagga
    23581 aaagagtttg aattgctcgt ttatttttta caaaataagg gaatcatttt gacaaaagag
    23641 cagatatttg accggatctg gggatttgat tctgatacga cgattacagt tattgaagtt
    23701 tatatgagca acttacgaaa acatctgcga agtactggat tggataaaga aatcaaaacc
    23761 ttacgtaatg tcggttatat actgcaaagg gtgtgatgca atgaatggag atattacacg
    23821 aaagcaacgt agtcgctttt ttgcgacaaa tgtagcagca tttgctttta tcttttttct
    23881 tttgggattc attactttac agatattaaa ccaatctgca tacagagaaa cagatctgtc
    23941 attagagaaa ataaattttg attctcgaac agtacaatta gagatacaaa gatatcagca
    24001 aggcgatccc tttttaaacc cgactcaaac ggaacaaaac agaacaaaca tggaacagcc
    24061 attaatggaa ccatcaaatg accgttttaa cactcaagtg atcttgtggt ctaaaaatgg
    24121 tgagatattg aataaagcag ctattggtgg gcgtttcaac caaatcaaag aattaaaaat
    24181 ggatacgaaa aatctgaata ccatccaaaa tattgaatta aatgaagaca gtggctcatc
    24241 tgccctctct ttccgttcga ttacgaaaaa agcggagacg aatcaagatg gcgttgccta
    24301 catccaaatt ttagaaaata caaatcaaat caagaattca ttagcgactt ttcgcacgat
    24361 tatgattttg tgtatgattg ttttttggat tctctctatt ggtatcagtt attacttgtc
    24421 tagtctaagc atgcgcccta ttattctttc ttggagaaga caaaaagaat ttgttgagaa
    24481 tgcttctcat gaattgcgca caccgttaac gattattcaa aatagtttgg aacatctttt
    24541 taccaagcct aatcatacta ttattgaaga atccgaaagc atcgctcaag cattgtcaga
    24601 gacgaggcgc ctcactggat tgacttcgga tttgctgaca atcgctcgga atgattctga
    24661 tcagcagctg ctttcaaagc aaatgatcaa tacacaagaa tatatcgaac aacttgttaa
    24721 accatttcaa gagatggcca tcatagatgg aaaagagttt attctccata acgatgcaac
    24781 attagaagta ccaattgatg aaaaaaaaat ccatcaagtt ttggttattt tgctggataa
    24841 tgcattaaaa tatacgaatc cagaagataa gatcattttg gattcatctg ttaaccataa
    24901 agaatggctg attcacgtaa agaatactgg cccttctatc agtgaagaag atcaagcaag
    24961 gatttttgat cgattttatc gggaagatca atctcgggcg aaagagactg gcggatacgg
    25021 gcttggcttg gcaattgcaa aacaggtaat cgaacagcat catggacaga tttctgtatc
    25081 caattatctt ccacaaggag tcgattttaa agtccaattg ccgttgaagc ctaaaaaata
    25141 atgcttatcc ctaaaaaagg gctgtgacaa aaatcttgtc acagcccttt tttgttgcga
    25201 ataaacggtg ttccagaagt agcttcttcg gaaataagcc gaaatttctt taagctgacc
    25261 acttctgtca caacctcttt agcttacgct tacgattttt tattgcggtc ttcaatgtgt
    25321 acatcaccgc ttgtgtcaat atccaattct tctcggctaa catcttctgt cactttttca
    25381 acatcttcat gttcttcttt atgaatccca acttcttcac gaataactgt atgtttgctt
    25441 acatctactt gctcttcttt gattgggata gtaatcgtgt catcttccaa tttttcgtct
    25501 gttccctgtg aagattgatc tgttactggt ttgcgttcga tgacgatttc ttcacgtttg
    25561 accggtactt ctaccgtttc agtatcgttg accacatgtt tgtggatgtc gacttctcca
    25621 gttgtcacat catgtgtatt tacatctaat ttttcttctt ttagttgaat tgtatcatca
    25681 tctagatttg gtttgcgatc gatgtcttca gtcttgcttg tacccattgt atcagtctcg
    25741 ccaggtacgt ccatcggatc agttcctggt gtcatattca atggatccgt tgtattttga
    25801 aagcctgtcg tgccacctac gtccattgga tctgttccag tgccagtttc taatccagtc
    25861 cccattgtgc ttgctgggct aacagtatct tgtgtatcca ttgtatcttc tgttgtttct
    25921 ggctgacgat agccttttac tgtgatgaca aattttccat cagaaatatc tcctctatat
    25981 tgatataaag gatcattttc tggtgtagcc gtctcagaat cataggtatc aaaagaaaaa
    26041 gtatctttta ttttttccca taaagaacgg tcttcttcac catccatccg attttcatct
    26101 gtttcaacat ctataccaga caatccgtca aatcctagtc tagatgctgc ttcatcagta
    26161 gtataaataa cgatatcatc tcgttcatac ccttcgttac gcaaccgttc aattactgat
    26221 aatgcttctt ccctcgtact atagcttccg atgaccgtta aatttttctc ttccatgtaa
    26281 attcctccta gcaaatagat ttatgattgg tatattgtta cttataaatt tattctatag
    26341 gagacgattg ttttctacaa atatttagat tggaatttga ttaaaacaat ttaattgaaa
    26401 tttgaaaaca tttatgttaa tgaaagtcat attctaataa aatgtcattt gtttctatat
    26461 gaatttttat gaaaaatttc atataatttc cactcgttgt gatggtttaa tttatccaat
    26521 ccatcaataa gcaaacaact tatataaaaa aggattaatt ctcaatataa ttgctcctac
    26581 catagaataa atcttcatgt aaagtaatga agtaccaact atttctaata cttctttaag
    26641 aattatccca acaatatcgt tagacaaacc attattgaat aaatggagag ccaatcgatt
    26701 ctgaaaaaat gttttctaat ttttcggaaa taaaagaaag agcctcctgc cattagaacg
    26761 aaccaaatcc atttcattgc tcatgaagta accacatagc taaaaagagg ataaatcaat
    26821 taaaagatca aaagaatgac tgcactaata aaataactaa aaatgtatct tcttacttca
    26881 tatcttttag ctagtactta gttaaaaatt tgcttcctaa gatcaagaca aactgtctat
    26941 cttcatttga tgaaatgcgg taaaatatag aaaatgaaca ctattggagg gaatttgtaa
    27001 tggacagcaa aactagaaaa caactgcaag aaaagttaaa gcaacgtaca cacttgctcc
    27061 cttccgtctt tcaacagttt ttagaagaaa atcgttcttc cctttcatta ggcagtcgct
    27121 atgaatacgc aaaagatttt catctgttta ctgattattt gagaaaagta ttttctgaag
    27181 aagtggatga tcagctgatt atccatttag aaaaacaaga ttatcagaat ttcttagcta
    27241 aaatctatcg acatactcgt tcgttccaaa ctacagcgaa ccaagaaacg gagcaattgt
    27301 ttgaaaatag ctattacggg atttcaagga agcaatattc attaaatcat tttttacgat
    27361 ttttgtatca aaaaggactg attgaagaag agatttctct tttgggtaca agtttaccgg
    27421 aaacaaccaa agcagctccg cgatatatcg atgcagaact tttcaaagat tttgagcatt
    27481 tgtttatccc gattgaaggc tttcaaacaa gcagagaagc tagctttgaa gaaaagaata
    27541 gaccgaggaa ccttgcaatt actgccattc ttctgtattg cggattaaag atccatgaag
    27601 tagtagaact taaacgtaaa gatgtatctt tgaccaagca gattcttaat ctgaatagaa
    27661 aagaaaacag actgaataaa gtaccaatac caaaagaagc gatgccattt ctggagaact
    27721 attttaagtt aacagcacaa aatacggacg aaaattcttt tgtttttcgt tcctcacatg
    27781 atcagcaaat ttctcctcgg actattcgcc aaattttgag caaaattaca gtatataaaa
    27841 acgtatcgcc agaactatgc agaaaaacat atcgttatta tgcagaacta tatttagatg
    27901 atgctgacgc ggtaaattat ttatgtggta atcgatacct ctcatcccat tcttttcaag
    27961 atatctggga aaagatggtt gatttttctt atcacgaatt agcggcattc gttcgggttt
    28021 cttccatata aaacaaatgt atgatctttt tttcacaata aactatgtta ttatataaag
    28081 gattatgtta cgattatctt gaatttgtat attacttaac aaaagataat ggagtgtgaa
    28141 tgatggagac cataacgtat tcccctttga acttatttac gaattttcaa gaagctgcag
    28201 ctaacttttc cgagaccccg attatctttg atgagccact ttcagctttc ccagaattag
    28261 gacttgaaac aacttacaaa aaatgctgcg aaagtgtgtt gaaacgggct tatcaattgg
    28321 ctcatcttgg agtgaaatcc ggagacaaag ttattattta taagagttcc gcttttgaca
    28381 cataccttct agctgtttct gtgtcttacc ttggtgcagt gcctgtcatg acttcttatc
    28441 atctaccaac aacaacaatg gaagtattta tcgatcgttt agaagaccct ttcattttat
    28501 ttgatgatga gacaaaaaag cgagtgagag aaatttcaaa tggaacaaaa tcaaaacaga
    28561 ttcc
//
LOCUS       DACDIE010000032        27900 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.032, whole genome shotgun sequence.
ACCESSION   DACDIE010000032 DACDIE010000000
VERSION     DACDIE010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27900
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.032"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            126..773
                     /locus_tag="IV250_001545"
     CDS             126..773
                     /locus_tag="IV250_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAP8768835.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            857..1801
                     /locus_tag="IV250_001546"
     CDS             857..1801
                     /locus_tag="IV250_001546"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HAP8768836.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTIQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            1936..3387
                     /locus_tag="IV250_001547"
     CDS             1936..3387
                     /locus_tag="IV250_001547"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAP8768837.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            3408..4244
                     /locus_tag="IV250_001548"
     CDS             3408..4244
                     /locus_tag="IV250_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP8768838.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            4244..4903
                     /locus_tag="IV250_001549"
     CDS             4244..4903
                     /locus_tag="IV250_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP8768839.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            5029..5718
                     /locus_tag="IV250_001550"
     CDS             5029..5718
                     /locus_tag="IV250_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8768840.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            5724..6680
                     /locus_tag="IV250_001551"
     CDS             5724..6680
                     /locus_tag="IV250_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAP8768841.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHRIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            6724..7236
                     /locus_tag="IV250_001552"
     CDS             6724..7236
                     /locus_tag="IV250_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8768842.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(7492..8040)
                     /locus_tag="IV250_001553"
     CDS             complement(7492..8040)
                     /locus_tag="IV250_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8768843.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(8129..9310)
                     /locus_tag="IV250_001554"
     CDS             complement(8129..9310)
                     /locus_tag="IV250_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAP8768844.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(9546..11054)
                     /locus_tag="IV250_001555"
     CDS             complement(9546..11054)
                     /locus_tag="IV250_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAP8768845.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            11213..11404
                     /locus_tag="IV250_001556"
     CDS             11213..11404
                     /locus_tag="IV250_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8768846.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            11607..12524
                     /locus_tag="IV250_001557"
     CDS             11607..12524
                     /locus_tag="IV250_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HAP8768847.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            12517..13590
                     /locus_tag="IV250_001558"
     CDS             12517..13590
                     /locus_tag="IV250_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8768848.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            13738..14256
                     /locus_tag="IV250_001559"
     CDS             13738..14256
                     /locus_tag="IV250_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8768849.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            14237..14629
                     /locus_tag="IV250_001560"
     CDS             14237..14629
                     /locus_tag="IV250_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAP8768850.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            14705..15640
                     /locus_tag="IV250_001561"
     CDS             14705..15640
                     /locus_tag="IV250_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAP8768851.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            15773..16552
                     /locus_tag="IV250_001562"
     CDS             15773..16552
                     /locus_tag="IV250_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAP8768852.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            16545..16862
                     /locus_tag="IV250_001563"
     CDS             16545..16862
                     /locus_tag="IV250_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP8768853.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            17106..18716
                     /locus_tag="IV250_001564"
     CDS             17106..18716
                     /locus_tag="IV250_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP8768854.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            18873..19721
                     /locus_tag="IV250_001565"
     CDS             18873..19721
                     /locus_tag="IV250_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP8768855.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            19952..20431
                     /locus_tag="IV250_001566"
     CDS             19952..20431
                     /locus_tag="IV250_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8768856.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            20464..22692
                     /locus_tag="IV250_001567"
     CDS             20464..22692
                     /locus_tag="IV250_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP8768857.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            22821..24464
                     /locus_tag="IV250_001568"
     CDS             22821..24464
                     /locus_tag="IV250_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP8768858.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(24537..25082)
                     /locus_tag="IV250_001569"
     CDS             complement(24537..25082)
                     /locus_tag="IV250_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HAP8768859.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(25105..25590)
                     /locus_tag="IV250_001570"
     CDS             complement(25105..25590)
                     /locus_tag="IV250_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP8768860.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(25580..26392)
                     /locus_tag="IV250_001571"
     CDS             complement(25580..26392)
                     /locus_tag="IV250_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HAP8768861.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(26531..27643)
                     /locus_tag="IV250_001572"
                     /pseudo
     CDS             complement(26531..27643)
                     /locus_tag="IV250_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
     gene            complement(27664..>27900)
                     /locus_tag="IV250_001573"
     CDS             complement(27664..>27900)
                     /locus_tag="IV250_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAP8768862.1"
                     /translation="PRKNMDKSEKLDHSLLGKQRKTVETVFSSLEKLGCQNFNSRSVK
                     GLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         9125 a         5076 c         5568 g         8131 t
ORIGIN      
        1 tagtttcttt ttctttatat tttcataata aaagtttggt tctttaattt ttaaacatga
       61 cagaaaattt tttcttcgtt atacttgtaa tagaatacta ttttataaca aaggggatcg
      121 aatcaatgaa aaaaactgtt atttatacta gtttatttgc agctttgttt ttagctgctt
      181 gtcaatcaaa taccaatcaa ggcacatctg ggaatacgtc ttccagtagc gaacagcgct
      241 cttcacaggt atcggactcc tcaacaaaag caagcacatc ttcgtctagc cagacaaaaa
      301 gttctagtga agcaagtact cagtcagata tcaaagtgtc tgcagctcgt gcgatcgaat
      361 tgttccaaga taaataccca gatactgcaa ttactagttt ggaattagat agtgattggg
      421 gatcttactt ctacaagata gaaggtgtcg atgatcaaaa agaatacgaa gcaagaattg
      481 atgctcaaaa tgaaaaaatg gagacagaga atccagaaac tctagatcga gatgaacaaa
      541 atggtcaaaa gaaaaaagaa gatggtctgg atgttgataa attgatcacg attgaggaag
      601 cagcaaaaat cgccgtcgac aaagtaggaa gcggtacggc aacagattgg gatttggata
      661 aagatttagg tactacttat tgggaagtca aagtaaaaga cggcaatcaa agtacatcag
      721 tcaagatcaa tagccaaaca ggcgaagtac tgtcaactga acaagatgat taagaatttc
      781 ttcggaaaac aagagttgat ttttctttta gtcatgatac actagacaaa ataacttgtt
      841 tgaaggagcg atttacttga gtttagaaac gacctttgcc aatcatatct ttgccaatcc
      901 tttgatgaat gcatctggtg tccactgcat gactatccag gaattagatg aactggctca
      961 ttcagaagca ggagcattta tcactaagag ctgtacgatc aatgaacgaa aaggaaatcc
     1021 cgaaccacgc tattttgatg ttcctttagg gagtatcaat tctatgggac ttccaaactt
     1081 aggtttttct tattatctag agtatgctct tgcgtatgaa aaagtgcagg aaaaccaaaa
     1141 tcaaccgtta tttttttcca ttgccgggat gagtgtccag gaaaacttgg aaatgcttga
     1201 gaaaatagaa aaaagtgggt ttaatgggat cactgaatta aatttatctt gtcccaatgt
     1261 gcctggaaaa ccacaactag cttacgattt tgaggccact tatgaaacac tgaaagaagt
     1321 attcagcatc ttttctaagc cgctaggaat caaattgcct ccttattttg attttgctca
     1381 ttttgatcag atggctgata tcttgaatca gtttccattg acctatgtca atgctatcaa
     1441 cagtgtgggc aatggtttgt atatcgatac agaacaagaa gcagtagtca tcaaaccaaa
     1501 agaagggttc ggcggcattg gcggagagta tatcaagccg actgctttgg cgaatgttcg
     1561 cgctttttat acacgtttga aaccagaaat ccaaatcatt ggtacaggtg ggattcggac
     1621 aggacaagat gcatttgaac atctgctatg tggcgcaagc atgttacaga ttggtaccga
     1681 gcttcataaa gaagggccgg aaattttttc tagaatcata aaagaattaa cgcagattat
     1741 gtcagaaaaa ggatatacgt caattgacga atttaaaggc aaattacgca ccatttcata
     1801 gcaaagagac gaaggtcatg attgatcttc gtcttttttt ataaaaaaca ctcgtctgtt
     1861 cattcctgtg caagcgtact cattgtatgt tagacgaact tgtgggaaaa taacagaggc
     1921 aaaaaggagg ataatatgaa tgatgcaaca gaattagcaa aagagcttaa agaaaaagaa
     1981 tttagttttc aagaactagt tacggatata cagaaaaaaa taaagaagca aaatcatgaa
     2041 ttgaatgcgt ttgtcgcgct tgaaccattc gagagtaatt cccacgagct attgattccc
     2101 cagtcagaaa aagggccatt ggctggtatc ccatttcctt taaaaatgct cggtcaagaa
     2161 aaaaagggat ggctagctac ttcaggatct cgtttatttg aaacacaccg agcttctcat
     2221 acatctaatt atgtgcggca agcagaagcg ataggtctag ttccttttgg acaaaccaat
     2281 gcgccagagt ttggtttcaa aaatattaca gacccagtca tctatggccc agcaagaaac
     2341 ccttggaacc ttgaatgtac accgggcgga tcaagtggag gagctgctgc tgcagtttca
     2401 tcaggaatcg ttcctttagc tggtgctagt gatggcggtg gttctattag aatacccgct
     2461 tcgttttgcg gattgattgg gctgaaacct tcgcgaggga cgatgccagt tggtccatat
     2521 gcttggagag gatggcaagg agctgctatt gatttcggct tgacagtttc tatgagagac
     2581 acggaggctt tattttatgg gatgagaagt atccattccg gtgcgccata tcaagtatcg
     2641 ccagttgagt ggcaaacaca tccaagaaaa aaacggttga agatagcttt ttgtgttgat
     2701 tcaccgatcc actcacagat tagtgaagag gcagtacttg ctgtaaaaaa agctgtagat
     2761 ttccttggaa aagaagggca tgatttgaca gagattcctt acccactaga tggtcgtaaa
     2821 ctgatcacgt cttattatca gatgaatggc gcagaaacag cagcaatgat ccgctcgatt
     2881 gaaacaggca tccagcgagc tgtcagaaaa gaagaacttg aacccatttc ttggactttg
     2941 ttccaatatg gtcagaagct ctctgccgct acttacatcg attctctgca tgtatgggat
     3001 gaagcagcaa ttgtcatgga aaacctacat caagaatacg atctattcct ttcaccaacg
     3061 actgcattta ccgcccctaa aatcagcgaa gatttgcaaa gtgatgcgat aagagcacag
     3121 atggaacaca tcagtgattg ttctgaagta gaaggtttag acgttgtttc ttccatgttt
     3181 gaggagagct tgaagatcac accttataca cagttggcta atctgactgg acaaccagca
     3241 atcagcttgc ctactcattt gacaaaagaa aacatgcctc taggaatcca atttatggct
     3301 gccagaggaa gagaagattt gctatttcaa atcggctatc tatttgaagc aaatcatcag
     3361 ttccatttac caaatccatg gaaatagcag aaacaaggag aagaagaatg aagaaaaaaa
     3421 gattgtttgc tctaataact agtgcctttt ttattttaag cgcttgcggt acagctgccg
     3481 ctaatcagga agataacagc tggagtaatg tagaaaaaaa gaaagaatta gtagtagcga
     3541 cttctggcac actgtttccc gcatcttatt ataatgatca aaatgaatta gttggttatg
     3601 atgtagattt ggcaaaagag gtggctaaac ggttaaatgt caaaatctcc tttaaagaat
     3661 acaacgtgga tggacaaatc acttctgttt caaaaagaga atcagatttt gctgcgaacg
     3721 attttggtat cacaaaagaa agagaaaaaa aattctccct ttcagagact ttcaaatatt
     3781 catttgacag tatgatcgta cgaaaaaaag atgattcagg aatccactca cttgaagatc
     3841 ttaaagggaa aaaagcagct ggcgaaccca atactagcta tatgcgttta gcggaaaaac
     3901 acggagctaa attagtcaca tatgacaatg ccacgaatga tcagtattta agcgatgtag
     3961 caaatggtcg aacagatgtt attctaaatg attattatct gcagaaaatg gccgtatctg
     4021 ctttaccaga tatccctgta aaaatactgg agaacgttta tttcaatgaa aacgaaagtg
     4081 gttttttatt gaaagaagga aatgatgctc tgaaaaagaa aatcgatggc gtattaaaag
     4141 agatgaaaga agatgggacg acaaagaaaa tatctgaaaa atattttcaa actgatgttt
     4201 cagtcaaacc gaaagaaacg atggctgagt cggtgagtga gtgatgtact ttcctctaat
     4261 tgatttttcc ctcatgtgga cttcacttcc tttcgtactg caaggcttgg tatacacatt
     4321 agggattggc ttcgtgtcgt ttcttttagg aaatctggta gggttgcttt tgacagtttt
     4381 aggtcttttg gattggctgc ctctgaatgt atttattcgt ttttatttat cgttctttag
     4441 aggcattccc gcactagtcc tgctgtttct gttatatttt gggttacctt atcaattaag
     4501 tgccttaacg gcttcagtca tctgctttac gattaccagc agtgcattta ttggtgaaat
     4561 ctatcgagga tcactagcag gcgtttcttc aggtcagtgg gacgctggtt tagcttctgg
     4621 tttttctttc tttggggtat tgaaatatat cgttttgccg caagcttttc gaatctcagt
     4681 accggcactc agtaacgtag ctatggatct agtgaaaggt acttcgttag cagcaacaat
     4741 cacagtgcca gaaatatttc aaaaagcaaa gatcataggc ggtcggacat tcgattattt
     4801 ttctctctat attttagttg cactgattta ttgggtcatt tgtatcggga tcgaaaaagt
     4861 gcaacagtat ttagaaaaac gttctatgca tcagtcaatc tgaaaaatag aaatggtgct
     4921 agcgtggctg gcaccatttc tattttttgt ttcttgtata ttctgcctag tattttaaaa
     4981 ccatttcatg taaaatgtaa atcgaaaata tgttcggagg tagaaaacat gaagaccccg
     5041 ctaaccatcg aaatagaatc ctcgttatac caatattgca tcgagcaagg cggcttggtt
     5101 gtagaagaag tcacgatgcc tgatgaccaa ggaattgtag atacgcttag ctgcttgaca
     5161 aaatttgatg gaagccgaga atggcgatgc tatgagctga aagtgacaaa agcagatttt
     5221 cgctctacag ccaaactgtc atttgttgga cattataatt attttgtttt accaagtaaa
     5281 ctatatgaag aagtaaaaac tgaaatccct acaggaatag gtgttttagt gtatcgtccg
     5341 tacacggttg aagaggagat gcctgcctct ggtacatttc tgattgcgaa aaaagcagca
     5401 aagaaagagc ttgctgtacc ggaagaagct ttgacaaacc gcttcatggc ttctttattc
     5461 agggaagtaa gaaaagcaaa gcaaatggag cgaggcgtaa aatattttcc aacagatcaa
     5521 ttgtacaaag aattgaaaag acgttcagaa aatcaagatc cattttctaa cgaacgatat
     5581 tatgaacggt ttttagagga aatagctagt caggcaatca atgagttgca agaagaagtt
     5641 atcgcgcttc agcatgatta tgaatatttg aggcaacaac agcagatccg tcggttgcct
     5701 acagagccat tggagtgaaa aatatgtact atccttattt aagaggaaaa caatttgacc
     5761 tattagcatt aaaagaagca ctgtcgcgag gattgctctc caataaaatc caaccagtga
     5821 tcgaaccagt acgtgattcc gctacgctta aaaatgtgat cgaattattt caaaaaaagc
     5881 accatcgaat cgctgtgatc caaaatcccc aagtaggaca attcaaatta ttcgatcagc
     5941 atgtgcacag ttggacagtc aaagaaaata gctctgtcgt ccctgcacag atcttaacgc
     6001 cagaaaatct cagtgaagta ctggattcac ctccagcgtt tcttgttttc gatggacagc
     6061 actacccaaa agatacagag gtgtggaaac agttggcagg agttgattcg aaatttttga
     6121 taccagatac tagtcgcttt cgcatctggc tgcccgaaaa caaaattgtg atccgtgact
     6181 cttttcaaac gagaaagcat gttgaaagtt atgcagataa aacagatgac ttttttagtg
     6241 atgactatct ttttttccac gcagacggat atattggatt ttctgatttc acgatcgaag
     6301 gtagccgtta tttcgataaa gggttcccta gccgagcgct tgctatccat ctaacgtata
     6361 ttgatgctta cggcaatata cgcgtgaaac attttgtctc tgacagtaac gactctgcta
     6421 aagaccaagc attgaagttt cttgaagcag gagataaaat gaaaaaatgg atcatgcgtc
     6481 accatcatca actcttgatt acttcaggaa tgattgaatt attggctctt taccagcaac
     6541 aaaaattccc tggactggga gtgttgaaaa agtggtcact tatgcatcat ttggaactta
     6601 ttagtcacct gctagatcat ccaaaagatt ggcttaaata ttattcaaaa gttcaggaac
     6661 tatatgaagt gatccaatga ttagataatg taaatgaaaa aggaatagaa aagaggaaac
     6721 tggatgtata ttcggcaaac aaggaaagaa gaagtagaag aagtggtaga aatcattaat
     6781 gccgcagcgg atttcattgc ttcgcaaggc tctcctcaat ggcaaaacgg gcagaaacca
     6841 acaaaagaaa cgattttgaa cgatattgaa agaaaagaaa gctatgtatt aatagtggaa
     6901 gatcaaattg ctggaacagc tgcattagtc tcaagcgtgg atcccgttta cacaaaaata
     6961 gatggtagtt ggaaagaatc aactggtcct tatttatcga ttcatcgtgt cgctttagca
     7021 gaaagtactc gaggaaaaaa actagcaaaa ccgttcttag aacatttgtt gacgattgca
     7081 gccagcaaaa atatccaaga tatccgtatc gatacttatc cagctaatga accgatggaa
     7141 aagaccatct attctgctgg attcacttat caagggatga ttgaatttcc ttttgaacac
     7201 ggcgaaagaa aagcatatca aataatcatc aattgactat aaaatcatct gagcaaagat
     7261 aaagacatta acactactga aaaaaacaat catatgatag ccaagaggtt gtaacagaag
     7321 tggccagctt caaaaaacaa gaaagaattt ccgaaaatgc ttctcaaatt tttgtggaat
     7381 tttagcgtat tttacgaaat agctgctttc tgaaactccc ttcatcgaga ggtcgtgaca
     7441 tagcttttgt cacgacctct cggcttatcc tacgagtatt attcgataca tttatatcaa
     7501 taatcctttt tttattttat ctgcagcttc ttggaacgct ccatcgtttg acacgactaa
     7561 aagtgagggg atagctttca aacgtttcaa ctgtgctaaa tcttctacat aaaccgccag
     7621 tctggctgaa atctgttcag atgaatctcc acgttttttc aggcgcgatt gcaatgtttc
     7681 tttagagatc gtaataaaga taggacggac taccgtcccg aaatactcat ataaatgcca
     7741 aaaaccattt gctgtcaaaa cggcgtaaca atttcctact gtcagtttct ttttaatttc
     7801 tgctttactg gaaccatatt tatgttgagc atattggtcc cactctgcaa actcatttcc
     7861 cttgatcatc tgattgaatg tattgtcaga aacaaaataa taatcctctt gatctttttc
     7921 cccaggtctt ggtgatcgat tcgtgtaagt aatgatcttt tgttccggct gaaaaatagc
     7981 ttctgcgagt gaagtttttc cagaaccaga aggaccgata aatacatagc aataattcat
     8041 tgtcatttct cctttgatga ttgaacaagt gaaaaagctt tggaaaatat ttccaaagct
     8101 tttaaaagta actttgcttt tttgtatatt ataccagcaa gccagtcaca atagcactta
     8161 aaaagctgac taaagtcgct gttagcaaaa ttttcaatcc gtgttttgcg acaagatttc
     8221 ctttttcttc atttaatccc ttcatcgcac cagaaatgat cccgatagaa gagaagttgg
     8281 caaatgaaac gaggaagaca gatataattg ctgttgtacg ctcagtaaaa tggaaattac
     8341 catttgctaa ttctgtcata gcaacaaatt catttgtaac cagtttagtc gccatgatac
     8401 taccggcatc cactgcttct ttccatggga ttccgctgat gaaggcaaga ggggcaaaga
     8461 tgtaacctag gatctcttgg aaagtgatgc cgaatatccc gtggaagata gcattgatca
     8521 tggcaatcaa agcaacaaaa ccgataagca tagcagcaac cgtgatcgcc acatggaaac
     8581 catcaagaat gtattctccc aacatttgga aaaatgtctg ttctttgttt tcttcaatga
     8641 tcagctcatc ttctttttct tctaatacat agggatttac aattgaagcc agaatataac
     8701 cgccaaataa gttcaaaaca agggcagtga tcacatattt agaatcgatc agtgccatat
     8761 aagatccgac gatcgacata gaaactgtag acatagccga tacactcatg gtgaacaaac
     8821 gtttctcaga taggaagggt aattcctttt ttatggaaat aaaaacttcc gattgtccta
     8881 aaatagcaga agctactcca ttataagatt ccaatttgcc catcccgttg attttactta
     8941 atcctagacc aacagctttc ataaataatg gaagaatctt gatatagcgt agaataccga
     9001 taatagcaga aataaataca attggcatca atacggaaag aaagaaaggt gcacttcctt
     9061 tatttgctac accggcaaaa acaaagtcaa caccttgccc agcaaaagca agaagatgat
     9121 caaacatttt tgccaataca gaaacaaccg ctgtaccaaa agttgtacgt aataatacga
     9181 aaccaagaac aagctgtaaa acgaacatca caattagtgg tttcaatttg attgcttttt
     9241 tgttactgct gatcaaaaaa gcaagcccta atgtgacaaa taaaccaata atacctaata
     9301 aataactcaa ataatcactc ctattccaaa ttccgtttta ttttacctgt tttgcaaata
     9361 aaatactaat ctttagtgaa attttcatta cttttttaga gtgaaacgca ttcattttcc
     9421 attagaaaat aaagcgaaca aaataaaagg tttaaaaagt gtaacgtctt agtaaagtct
     9481 ataaaaaaac gaaactatcg attaagctaa tagtttcgtt ttcaacattt ataagtaaac
     9541 tttttctatt ggtaatatag attttcggaa gggtagacag gtgcgcaggt aatatcgatt
     9601 cctagttttt tcaatgtttg ttcatcattt ttattgacca tcacagtaga gtgggcttgt
     9661 gctccctcaa gttcagctag tttatcataa gcaacttgag cgattggatt cgttactgca
     9721 ctgattgata aagcgatcaa gacttcattg atgcttagtg catccaaaga ggaatgaagt
     9781 gtctttgatt tcaatccttg tattgtttca agaactaatg gtgacaataa aggaatgtca
     9841 tctgcgatat gggctaaatg ttttactgca tttaagataa ctgccgctga cgcatccatc
     9901 aatgaacttg tacgcccggt aatgatgact tcatctggta attcgattgc aatcacagca
     9961 gaaggatctt ttgtaggact ttgcgcttga atcttatctg catagtcatg ggcagggact
    10021 accacgtttc ggtcttcagg acgtaaaccg acttcttcca taatcagttt gattcgatta
    10081 actgcttctt cgtcgatcaa tccttttttg aagtcgcatt ctgtttggaa ataacgacgg
    10141 atgatttctt gtttagaggc ttcttggaca acggtatcat ccacgatccc aaatcctaca
    10201 cgattcacac ccatgtcagt aggagattga taaacagatt ctttaccagt aattttttca
    10261 ataatacgtt tgattacagg aaatgtctca acatcccgat tatagttcac agctacttta
    10321 ttgtaggcat caaagtggaa ggaatcgatc atattgacat cttttagatc gactgtggct
    10381 gcttcataag caatgttcag cggatgtttc aacggaacat tccatactgg gaaagtttcg
    10441 aacttggagt aaccagctgc atgtcctttt tggctttcat gatacagttg gttcaaggaa
    10501 gtagcgagct ttccacttcc tgggccagga gctgtcacta caacgattgg tttagttgtt
    10561 tcaatatact cattttttcc aaaaccatct tctgagacga ttttttctac gttgactgga
    10621 tagtcttcga tttctgagtg tttatagacc ttgatgtttc gtctttctag tttattgata
    10681 aagactttcg tggcaggctg tccactatag cgagtgataa cgacactgtt gattaataag
    10741 ccataaccac gtaattcatc gattaaccgc aagatatcca tatcataagt aatcccataa
    10801 tcaccacgga tcttattacg ttcaatatct ccagcataga cgcaaatcag tatttcagct
    10861 tgatctttca atttttgcag caatttgatt ttcgcatctt cttcaaatcc tggaagtaca
    10921 cgtttcgcat gcttgtcgtc aactagcttc ccaccaaatt ctaaatataa tttgtcatag
    10981 tggttcactc gttccaaaat atacgctgac tgctcttcaa tatatttttc tgaatcaaaa
    11041 ccaatttttt tcactaggtt ccctccttaa gtactaaagc attttttcat ttttttgagg
    11101 attcgtcaac tggaagagcg aaattcctca aaaaaacaaa gaaaatggtt taaaccaact
    11161 tgtctagata gttattcatt ggtatgatag tttttgataa ggaaggtgct acatgaaaaa
    11221 aagacagaaa aagaaaaacg catataaaca ctatatccgt tcgattttta ccggttatga
    11281 aagaatgctt gaagatcccg aattggaaca attgacgttt acttatctaa acgaagaaac
    11341 gcagctaact cgtgatgagc atcagcgcat tcatttcaca acaagagatc tgcctagtaa
    11401 ataaaaaata agataaaggt catcggattc gattctgtcc tatcagatgg aaagactagt
    11461 tgatcgaaaa aaaggctgta ccaaattttg gtgcagcctg ttttatggag cattgcccgc
    11521 tcttttttat aacaattttt tacagaaaat ggtactattg aagtattaat aagtgaaaaa
    11581 cagatgagta agaggaggca agtagtatga aagagccaga tacaaatgca aattatgagc
    11641 cagacgaact agaggagacg aatgcggatc aggatacagg acaagaatcg tcttcttttt
    11701 acgatatcaa agaaaaagca agcatgaatc tttcccaatt attagaagac ttacaggctt
    11761 ttgaaaaagc tgtccgcgaa gaagatatcc ctgagatcta ccgtttatac aatggccgat
    11821 tgaatgaaga attaaaaaga agctcaaatg aaaatcacga gatcgatcaa ttgctgatgc
    11881 gtaagatcca tgatcgtttt ttacaggaat ttccttttat ggaacaagta gaaaacatct
    11941 cgccaacgat gagctattac aaaatcggca cttattacca tgatcggccc acggtcggta
    12001 ttgatgcaag tctgccagaa atttttgttt tgcctaagat cgatgaagag tgggagaaat
    12061 attcccaacc ggagtcaacg gactatgaca aaaaaatcaa tgagctagac gcaaaggtga
    12121 tcactgctca gacagaaatt gaacgtttag atgaacaaat aaaagaaatc aatcgacaaa
    12181 tgactgattt gaacgacaat aaaggattct tgaatcgtaa aaaaatcgag gaagaaattc
    12241 aagaattaga gaagaagaag caagtactat ctaatgaaaa attaggctgg cttccttata
    12301 ttgagacgcc tgaaacaatc cagcagcaaa aagaagcatt gaagcaagaa gcaagagcag
    12361 accagcttcg tgcagccatc gtagaaaaag aacaaaggca aatcagacgc tatttcggta
    12421 gcaaagaggg attcggtcaa gcaatccatg aattcttgat gaattatctt ggcaatgaga
    12481 atcctaataa tcaatctaat gaaggaggtt ccgagtatga atgaacacga tgaagcagaa
    12541 tggacaaaaa atgaagaaca aacagaagat catgaagaaa cgaacacttc attggaagaa
    12601 caaacaagta atcctctgga tgacacagaa gaaatcccta tgctagagga aacaaaaata
    12661 cacgaaaaag aaaaagtaac tccgacgaat acgaatactg cagaaaacac ccatgcttac
    12721 gaaataactg aaaatcccgg gtatttagaa ggaaaagagc aagatcatca agatatcgtc
    12781 agtttgattg aaacagcgga agcagaatta gcggatttac gtatgagaaa agatttgatc
    12841 gaatcagatt ttccacaatt attagaatca tatgatcgtt ttatattgaa tgaagaacca
    12901 atctccacga tcggaaagac aagcctgatc gaatacttca cgtatgaatt actgaaaccg
    12961 ttgaatgata ttggtctgga acggtcaaat tcagcttatg acacatggaa aacaagacat
    13021 ttttcgatca attatacgtt gaatggacaa aacgaattag tcttttcctt tgtattaccg
    13081 accatcgatc aacgatacca agtggaatcg atgagtctcc taaacgtatc accagagaca
    13141 atggaaatca atgtccaaga tgaccgggtg ctttctttga tccgacactg gtcagtggat
    13201 cgagtattct ctgcagggca gatcacgata ttcaatcaca aattgaatca attattagcc
    13261 catgcaagaa cacttggttt taccgttaat cagacattac tagacaatac gaagccactt
    13321 cgtttgaatc ttcagagcga atttgaattg acgaatgaag tattggatga tatttttatc
    13381 gtggcaatga aaaatccatc ctacgatatg gaaaagatca acgaaactac ttatcaagta
    13441 cgattagata aaggacagtc tttaacgata acaacaaacg aaaaagatca aacagtcttg
    13501 tcgatctcat ctggagacta cccacgatct gtcattgatt tctttatcaa ttacgaattc
    13561 ttggtcccat taatggtgag aaagtcctga ttggataagt tggattacca gatgtcattc
    13621 agataattct gttccaaagg acttgcggca gttatccaga ataattgtca caagtctttt
    13681 ctgtatgtac taaatggacg tgaataactt gctgatgaag gagggatttc attaggaatg
    13741 tccacaaata aaatagcaca gcttagctta ttgtctgctg cttgtgtagc aggtaggatc
    13801 gcttttcaat ttattccgaa tttccagcca atgactgcta tttttttatt tatagtactt
    13861 tacttaagct taaaagatgc gttaattgtc atgagtcttt ctattgcgat cagcagtttt
    13921 tattttggtg ttggaccatg ggtcatcggc caatggatca gctatactgt tgttctgagt
    13981 ttgttttcaa tcgtctgctt gagaaaaaca gtgcaaagga atctatggtt aaagggagtc
    14041 tgtttttttc tggcgggcat ggtttacgga ataggtatga ccgtttttga tacgctgtta
    14101 tatcgtttgc ctcaaccttg gatctattac cttcaagggg tgtcgtttga tttgatgcac
    14161 agtataggca atgttgtgtt ctttcttgtt tttctgccag tcgtcaaacg tttttacaat
    14221 cattcaggag gaaaaaatga aaaaaataat ttatagtttg cttgcaatga gtgtcagtat
    14281 actcattttg acaagttgca gtacaaccta tacacaatcg gcttccaacc aaaaggagga
    14341 agtaaaaaca tcgcaaaaag caacaatcac gttacaagaa aatggtcaaa acttttcgga
    14401 aaaagaagtg ttcttcaaaa aaggcgatga tttactgagt attttgaaac gaaattttga
    14461 agtcaaagaa gataacggct tcatcacttc gcttgaaggg cactcacagg atgagaaaaa
    14521 tcaactctat tggatgttta ctgtggacgg gaaaagtgct actactggtg ccaaagagat
    14581 caaattgaat gatggacaat cggtcatttt caatttgtct aaactataag tttcataaaa
    14641 aactgttaaa gggcgaatat tgccataatt cagtgagttc agttgatgaa agaaggaaaa
    14701 taacatggaa aattttaaaa aaagcggaat gttttccgta attgctgctc taggggctaa
    14761 tattctagtt gctatcagca aatttgtcgg ctatgcaatc agtggcagtg ccgccatgct
    14821 aaacgaaagc atccacagta tcgttgattg cagcaatcag attttgctgt tattcggtga
    14881 taaacaagcc aataaaggac agagcgagtt gcatcaattt ggtgaaggac gagcaaaata
    14941 ctttttcagt acaatcgtcg caatgatgct tttcttcggc ggtggggcac ttggggtgat
    15001 ggaagctttt gaaaagttga cgcatccatc ccatgaagtg ggtaatccga tcattgttat
    15061 agcaatactg ttatttggtt tggtaatcga aggaagttcg ttacgcgtgg caatgaaaga
    15121 aatcaaagaa ttgaatactg aaaaactttc cacgatgcgc tttttgcgcg aaagtcgaca
    15181 cagtgaaata ctgattattt ttacggaaga cttgtgtgct gtaatcggtt tggtcttggc
    15241 tttagcagga acaatattga cttatgcaac cgggattgct gcatttgatg cgatcagcgg
    15301 attgctgatc ggtttcttgc tgatgggagc cgccattttc ctagcaaaag aattttatag
    15361 tctgatcatc ggtgaaagcg tgacagcaag cgatttatca aaaatcaagt tagcttttga
    15421 acgtcctgag atcagtcggc tgatcgatat aaaaacagtt catttaagtc caacggagat
    15481 tttagttgca gcaaaagcag atatcgttgg taacaaagag aatgaagctt actcggtcat
    15541 caatgatatt gaaaaaaata tgcgtcagtc tcttcctgac aagaagatgt atatatacat
    15601 tgaaacagat aaatatgacc caaattattc tcgtaaataa aacgtttatt catgaattga
    15661 agcaaaaatg agaaaaatat aatcttgttt ttaaataaaa cggctacaat ttagcgaatt
    15721 tatgttacaa tgagtccgct gtttatttgt tgtaattaaa ggagatattt taatgaacaa
    15781 aaagaaaagt gcgccatggc ttcaagcttt gcatgcagtg atgccacttt gtataagcta
    15841 tataccagta ggattagctt gcggagtatt gttgcaaaaa gttggatttg atcctctctt
    15901 atcaggactg ttatcgattc tggtcttttc cggtggcgcg cagtttttag ttgcatctat
    15961 gcttacgacg caagcatcat ttgcaacgac attattgatg gttttctttt tagaattacg
    16021 ctatacatta ttaggagcaa gtcttgccgg ttttatgaaa aaagaaaagc ggacgttttt
    16081 ggctgtcttt tctcaatcac tgaatgatga aaactatgcg gtgaactact taaagttttc
    16141 aactgaccct tcctggaata aaagaaaggc tttatatgtc aattggttct caatgactgc
    16201 atgggccggt agcaatatga tcggtaattt tttcggctcg gtgattcgag tagatgctga
    16261 ccttgtccat tttgctttga cggccatgtt cattttcatg tttatcatgc agatgaaaag
    16321 cgcactactg atctttacag gtttattctc aggcgtatta ggggtcgtat tcatggtatt
    16381 attccaaaat actttcggat tgattgcagc aacagtcatt gcttcatttg ccggatttat
    16441 gctggagaag gcattcaaaa aagaaaaagc taagaaacta agagaccgcg gtaaagatgt
    16501 caatgagccg ctcttcaact tcccggaaca ggaggccaat caccatgact aaactatatt
    16561 tgctactttt agttgcttgt ctgttcatcg tggcatatat cccgcgcttg ttcccgatgc
    16621 tttattttac tcatcgaaaa gtgccatcct ggttcagtga ctggatgaaa tatgtaccgg
    16681 tggcattatt tgcggcgtta gcttttaaag atgttttcat tactcatgaa catctggata
    16741 ttgcttggaa tatcaaaata gctgcaatga ttctagtagc aggcgtggct tacaaaacac
    16801 gttcgatggc gttgtcagtt ttgacaggac ttgcgtctgt ctttttgctg tctatgcttt
    16861 aaactgtttt attttattaa aaacagagat tgcgacataa gttgttcaaa ctgatctatc
    16921 cgaactgcat ttgcaacagt caattctgga tgcaagttta ttcgtaggga taaaaaagat
    16981 tagtgttggc ttatgatgca agctcttttt tattttagga attagagagg gatttgtcct
    17041 tctatgattt tttgctataa tagggaagat gtttatttta tgaaagaata gttgagggaa
    17101 tttaaatgaa taaaaaattg atgcaaggaa cattttggct aacctttgct aatcttctat
    17161 gtaaagtttt aggggtagtc tatttgattc cctggctaag tatgatgggg aacaatcaag
    17221 atggtatgct tgctacgaca ttgtataatg ttggctatct tccttatgga ttgttcctga
    17281 tgcttggtac agtcggattt ccaaatgcga ttgcaaaaaa agtagctgta gccacaaaag
    17341 aaggagataa cgctgcttgt cggctgattt ttagaagtac gatcaatatc atgtttgtca
    17401 tcggtatcgc ctctgctgca ctgatgtatt tatttgctcc tttactagcc ggaatcagtc
    17461 cgattgctaa cgttaataat gggatactag ctatcagaag tttatgccca tctttgattg
    17521 ctatcccaat tcttagtgca atgagaggat atttccaagg aaaaaatgat ctgcggcctt
    17581 atggaacttc tttgattatc gaacaggctg tgcgagtgat cgttatcttg gctggtactt
    17641 attacttgcg cgtattgact gacggaacca tattagaagc tgtattgatc agtacagtcg
    17701 cttctttctt cggtgggtta gctgcgatta tccatatgta tatcgtcgga cgcagaaaag
    17761 acttcttcga acttaaagat tttttgattt ccagccgtta tttcgaaaga gaaaaccgat
    17821 ctgcctctgt ctctatcatt cgggaaacac taccgtttat ttttgtcggc tcagtcatta
    17881 ctatcgttca aatgatcgac caagtaacga tgaaaccact gcttcacttt ttccgtccag
    17941 agatcgcaga tcagcaactg gaattcttat tcagccgtgc ttctgtcaat ccaaataaat
    18001 tgacattgat cctgatctcc atggtaggaa cagtagctat aagcagcttg ccaatattaa
    18061 gcacgatgaa gaaaaaagat cggatacaaa tcgaaaaaac agtaggagat agtttttcga
    18121 ttgcgttatt gattcttctt ccttcattga ctggaatgtc tttattagct ggaccactat
    18181 acacattgtt ttttggctat gatcctgaaa gtgtcggata tttccaaatg gctttgctag
    18241 cttctttgtt tttctcttta ttcacgatcc tatctacgat gcttcaatct ttgaatcacc
    18301 atcgttttgc gatccgatta actgtagaag caattatcct gaaagtcatc ttccaagtaa
    18361 tcggattagg attagttggc ggatacggga tgagcttatc aaatggctta gcattcggta
    18421 ttgtctttgt tcgcggctat cactttatgt gcaaagaata ccgaatagca cctttagcaa
    18481 agatcagcaa ttttttctta aaaactttcc gctccaccct gattatgctt gcagtatgtt
    18541 ttgccgtttt ctttttgctg aatcagcgat tgtcaatgga atcaaaaagc tatgcaatga
    18601 tttattgtgt ggctgtagga agtattggcg gcttagtatt tttattttct caattcggaa
    18661 aaaatgggtt tcgaatgttg aaaaatgtta aacatcatag cagaagcaaa gaataggtta
    18721 actaatcgtt ttttgctgtc acttgaaaaa cggcaactcg tatgattacg cttgtcgttt
    18781 ttctattttc caattttagt ttcatatcta atcttactta tgatatagtt ataaggtctg
    18841 gaaaaattct ttgaaacgag gaaattaaac acatgaaact tgcagtagtt acagatagtt
    18901 cggcatatct gccggaacgt atcaagaacc atcccgactt attcgttatt ccaatccctt
    18961 tgattatgga tgggaaaatt tataacgaag gaatcgatat cgaagcggat gaatattatg
    19021 gacttttaaa aaacagtaaa gattttccta ctacttcaca acctgtcgta ggggaagttt
    19081 tgggtttata tgaggaattg aaagagaaag ggtatgacac gattttaagt atccatcttt
    19141 cttcagggat ttctggcttt gtcaacacat tatttgcgat gaaagatgac atcagcggag
    19201 taacggtcat tccttatgac tctaagatca ctagtatgcc gatgggacac atggtagaag
    19261 ctgccttaga cttaaatgat gaaggtcagt cccttgatga aatacttgca catatcgatc
    19321 gaatccgaga taatacctac gcttatctga ttgtagatga tttgaacaat ttagtacgag
    19381 gaggacgttt gacaaatggt gcagcgctga ttggcagctt attaaaaatc aaaccgatcc
    19441 tgacattttc tgaagggaaa attgttttat tcgaaaaaat ccgttcaagt aaaaaagcat
    19501 ttgctcgagc tgaagaaatt attggtagaa gaaataacga aatacaaccg ccagtcaaac
    19561 tttacgtgat acatgcaaat aatttagaag tagcgattgc ggagaaagag aaattgcaga
    19621 aaaaatatcc aaatgcagaa atcgaaattg gtcatttcgg accagtcatt ggtacacatt
    19681 taggtgaaaa agccatcggc attgccattt cagcgcaata attcctttta ttcaaaaaac
    19741 ataccaaaaa tattccaaca tcatcttgga ctttctacat gttactaatg agcgtgtaaa
    19801 agaacgagat ggtgttttta tttctatcaa tgaagctctt tttctttgca atcctcactt
    19861 caatcctcta taatgtcgca tatagtttag taaatgagta aactactaat ttacttattt
    19921 actaaaaata aaaggatgag gagttaaaat aatggaaata ccaacaaatt taaaacataa
    19981 accagtaatc gttgtagaag actatgataa agttgatgga agaaatgcat taaatacgga
    20041 tgcaaaaggg ctttcactgg gccttgctca gtggaatgat cgtggaaaag tcgatatctc
    20101 aggcaaggta tggcgacata ctggagaaaa atggtcacga cagtcggaag aattaccaat
    20161 cacacgtatt ttagatttag cgatcttagc tgcacaagga agtttatact tccaagatgc
    20221 ttatcgttat gaaaaatttt atgatccaga aaatccagtc gtagacatca ttggattaca
    20281 agggaaccga atgacggtag aagtagatac caaaaatcca aagatcgatg aagacatcat
    20341 tttgtattac gatacactac aaaaagacgg agaactgatt ggggaacgat tccgtatttt
    20401 aaaacgtctt ttagatgagc taggatatta atagacagag atgatcaaaa ggggaagaga
    20461 agcatggaga atttctcact aacagaaaac cgaacctttt cacggctaga aaagcaaatg
    20521 atctggaaga aaccgaaaag ccatcggaca agtgcagaag aatggcggat cgcctccgaa
    20581 atcaaaggga attggcaaga tcctgaaatg aaaatcagta acgtactttt ggaaggagat
    20641 gccgggtcgg ggaagacaca attagccaaa gcactatctg ctgatctgca acttccctat
    20701 acaaaagtga cctgttttgc agatatggac aaatcagatg ttttcggagc cttattacct
    20761 gtagtagatt ctaataagga acaagaccaa gaattactag aagctattta ccaaacagat
    20821 acacttgaag ctgtactgac actgatccag aaccattacg gcattgatcc tttttctgct
    20881 aaagaaaaat tagcagagct agttcaaagg atcgaagctg attatccaga tgcaatccat
    20941 tacagctatt acccttctga aatcgttcga gcgattgaaa aaggatattt attagagatt
    21001 caagagccga ctgtcatccg agatgcctct gttcttgtgg cacttaactc agcgctcgaa
    21061 ccaaatggga tgttgaattt accgacagga atcattcgtc gccatccaga ttgcgtcatt
    21121 gtgatcacta caaatcgaaa ttaccaagga aatcgcccat taaatgaatc tttaagagat
    21181 cggatgcaac atgcggaaaa aatggatctt cctgccttag aagtcatggc agaacgtgcg
    21241 atggcgaaaa ctatgatcca aaaacaagaa cttctcttaa agatggcaga gattatccgt
    21301 ttgttggatg aaactgcaaa agctaacgcc atcaaaggtg tagcaggaat gcgttcttat
    21361 ttttattggg caaataccta taagcaagga caagatttac tccagtcgat ttatccaaaa
    21421 gttttgtata aattatccac tgattcagat gaattggcaa ttcttgaaca agctttgtct
    21481 gacagtggat tgctactgga acttcaggac atattgagaa aagaaaaatg gggtcatcct
    21541 caatctgagc atacaaaaga aagaaccatt agcgccgaag aagcaaatga acgaaacatc
    21601 actgaaaatg ctgaagaaat tctgcgctta gatgaaaaga aatcgttgtc tgctgaagaa
    21661 ccaaatgagg gagaagagaa acaagaagaa acaatagaaa aaaacgagat tcctcctcaa
    21721 atgcaaaaag aacgcagtag cgatatcgaa ggaaaaaatc aaaaagaaca acaaacagga
    21781 gatgattctc aagaaatgtc tgcttctgac atggaagcac atgataagga aatcaagaag
    21841 caactgaata aagaagcgcg acaaatcatg aaggaaacga ttcatgaaaa agaaggtctc
    21901 atcgttcacc gaccagcttt ccaaggaaaa acgccggaag cacgtgagct acacatgcaa
    21961 gtaatgccaa tagtagaatc acttagccgg caaattttag agttactgga taatgaacaa
    22021 acagagacgt accaaaaagg aaaatacgaa ggtcagcggt tcaatgcaag caaagtagca
    22081 tatggtgatt tgagaaattt tgacaaaaag aacccgccac atgagcaacc ttctttagcg
    22141 gtagctgtac ggatcgatga atctgggtca atgatacggg atgatcggat cgaagcggca
    22201 aaaaaagcag cgattgccat tgccgaattt tctaagaaag tcgatatccc attgttgatt
    22261 tacggagata cagcagaccg ttcgtcaaga gaaaagactt ctttgttttc ttataaagaa
    22321 tttgaagacg gatttcaatg gttaaatgaa aaattagtca ctatgaaacc aaggcaaaac
    22381 aatcgtgatg gtgctgcact tcgtttagca gcagaaaaat taaacagaca atccgcaaca
    22441 accaaactgt tgcttaatat tagtgacggt cagccaaaag cgttgccgga ttatacaggc
    22501 aaaaaagcaa aagaagatat ccagtctgtt ttgaaagaat atgaacgtca aggaatccta
    22561 tttgtctccg cagctattgg ccaagacaaa gaagaaatca agtcgatcta cggtgaaagt
    22621 cgttttgtag atattactga cttgaatgaa tttcccaagc aaatgattca actgattgcc
    22681 cgctatctat gataaaataa agctcagcga acgcacttag cagattcgtt gagcttttcg
    22741 tttctagtta ccggtaacaa ggatacttct aaaatagaat cggttataat caagagggac
    22801 cgttaaagga gtgaaacgat atgatacaag aacaaaaatg gtggaaagaa gcagtaggct
    22861 atcaaatcta tcctgctagt ttcaaagata gcaatggcga tggtcaagga gacatcaatg
    22921 ggatccgtga aaaacttaat tatttgaaag atcttggtat cggctttatc tggatcaatc
    22981 cgatctatca atctccattt gtcgataatg gttatgacgt aagtgactat caagatatag
    23041 aagagagatt cggaacgatg gaagattttg atttattgct aaaagaagca catgaattag
    23101 ggatcaaagt gattatggat cttgtcatca accatagttc ggatcagcat caatggtttg
    23161 aagaatcgaa aaaatctaaa gaaagcccgt atcgcgatta ttacatttgg gtcgacgggg
    23221 tagatggcaa agaaccgaat aattggacat cgattttcgg cggttctgct tgggagtatt
    23281 ctcatgaaac tggacagtat tatctgcatg tttttgcaaa agaacaacca gacttaaatt
    23341 gggaaagcga gaaattgaaa gaagaactat tcaatatgat ccgatggtgg ttggataaag
    23401 ggatagatgg atttcgttta gatgcgattt ctcatgtaaa aaaagatgaa tattcagtaa
    23461 aagccacaga gaaccctttc tcaccctttc aaaatgtctc aggaatcgaa gaacatttga
    23521 ccgatttgaa acatgttttt gaagagtacg atatcatgac tgtcggagaa gcaagtggtg
    23581 tcactgcaga agaaggacca caatgggtag ggaaagacgg ttatttcgat atgatattcg
    23641 aatttgatca tatccatatc tggcagcaag aaaaagaagg gcaattagat gtccttaaat
    23701 tgaaacttgc attaagcgct tggcaaactt cattggatgg tatcggatgg aacgctttat
    23761 atatggaaaa ccatgatgtt cctcgtgccg tttcagtgtt tggagatact cgtcccgatt
    23821 tttgggctat gtctgcaaaa gcaatcgcta tgatgtattt ctttttgcaa ggtacgccat
    23881 ttatttatca aggacaggaa atcggaatga cgaatatgcc ctttgaatcg atcgatcaag
    23941 ttgatgcagt agattcgaaa agactttata aaaggctgct tgcggaaggt aagacaagag
    24001 aagaagcatt ggatatcatt cgtgaaacaa cgagagacaa cagcaggaca cccatgcaat
    24061 ggacttcgga acaatatgct ggtttttcta cacatgaacc ttggctgatc actaatccaa
    24121 ataccaaaac gatcaatgta gaacaacaag aatatgaacc agagtcggtc ctgcaattct
    24181 ataagaacat gatccgcatt agacaaacca ataaaggtct gatatacggt tcttataaag
    24241 aatatcttca tgaacatcca caactttatg tctatgaaag atatttagaa gatgaacaat
    24301 atcttattat ggtaaactta acagaatcac ttgctgacta tgaacttcca aaagaagcag
    24361 atcagtcctg gacattactc ttgtcaaatt cctctagcgg agaatttgaa gctaaaggca
    24421 tactagcacc ttatgaagct cggttatata aaacaaacaa gtaaaacttc ctcgttaaag
    24481 ttcattagtt ggactttgcg aggaagtttt tcagattgct ctgtcaatta ctcatttcaa
    24541 taattcgctc taggaccacc gatatattta ggacgagagc gaagggctgt cacgtgagct
    24601 tgtccaaggg ttttcaaacg aggtctgaca ggtacttgga catgtttgat atgcataccg
    24661 attgatgtgt caccgatatc gataccggct tgtccaacta cgtgttccac ttcgacagga
    24721 tcattgaact ggtcaaaagc agcaacagaa caagcacctc cagcatgtag tgcaggaaca
    24781 acagagacaa tttcgaaatt ttttgcttct gccactgttc gttctactac taatgctcgg
    24841 ttcaaatgtt cacaaccttg gacagctagc gcaatttctt taggatccaa caactctttt
    24901 aacgtacgga tgatccattg accaacttcc gcactggaat tttttccaat atgtcctcca
    24961 acgacttcgc ttgtgctgca tcctaaaaca aaaagatcac cttttttcaa gtgtgcctca
    25021 gccaatatgt cattaactat ttcagtcaat tggtctttta attctttttc tgacaacttc
    25081 attttctaaa accttctttt tcaattaata ttgcttccgt gcttgaatac gttgaagact
    25141 tgcagttata ggaaaggcaa gtcccattcc aatcagattt tggaagatgt ttcctggtat
    25201 agaagccact ccagccggcc aatcatataa aatccaaccg gcgataccat agccgatgat
    25261 cataactaag cttcctaata aaagccctaa tccccacata cgagtagaag atttgctgaa
    25321 agaaccagca acatatcctt gtaacccgtg gatcactaat gaaaaaactg cccactgcgg
    25381 atatcctgaa atcaaatcga tcaatagacc actggaagca ccaacaacta atccacccgt
    25441 tggaccataa agaaaagcag cggtatagat cccggcctcg cataatgtca caaatccatt
    25501 cgtagccggt acagggatca aaattgttaa tgaaagggca actgtcaacg caatcagcat
    25561 agcatagatc gttaattgct tagttttcat ttctcattcc tcctttttga ataccgccag
    25621 accagacact tccggtatga tcgttcatca gcacgccatt agaaatacaa tgggcaacaa
    25681 attttttact ttctgctact gctttggtaa aatctgcccc taacacaaga ttcgctgtta
    25741 ttgcagaaga aaaggcacaa ccggccccgt ttactgttgt agtgtcgatt tttttatgag
    25801 aaaatacctc ataatgatca attgaataga gaagatccac agcttcatct ccagccatcc
    25861 ttgtgccgcc tttgatcaca atgttttttg ttcctgaagc agtgagttct ttagcgagat
    25921 ggatcatttc tgttaacgaa gagggaagag cttgaccaga aagtaaagca gcttctgcca
    25981 aattaggcgt aatcatcgta acaagtggaa aaagttcttc gatgagctgc gaaatataca
    26041 attgattgat cacagcttca gtttctttga atgccaaaac agggtctaaa atgattggga
    26101 tattttgttt tgtttttatg aagtctcgaa caaccatgat tgcttcactt gaattcaata
    26161 acccaatttt gataccatct aatgaagtca ttttatcaat cgaaactaac tgtcgtttaa
    26221 gcaattccaa agatatagat tccagcataa atcgctcatt aatatctagt gcccctatag
    26281 aagtcaaggc gcttagtccg aatagaccaa aattctcaaa agtttttaaa tctgtttgga
    26341 ttcctccacc agcaaatgga tcagaaccgc caattgtcaa gattcttttc ataacatttt
    26401 cctccatgaa taagagtata acgaaatttt caaaaaagaa aaacagccaa ttggcatatt
    26461 tttatctcat ccaatttaaa aaaacaaaaa gaaaagaaag accaaaatgg atctttcttt
    26521 ccttaaatag ttaggacttt tgtgatttta attctttctc gatttgaagc cgttgcactt
    26581 tcattgtagc tgttcgcggg attgcatcaa aatcacggat gatagggacg ttcaagcgag
    26641 gaagttcatg gacttgctcc caccatcgat tccaatccat ttctttatca ggagctaaag
    26701 caataatcgg ttgtggagaa tgatttttat cccttacaat cactacttct gccaaaaaat
    26761 ctaacgaatc taataaaaag tcttcaatag ctaatgtact attgatcgtt tcgatcaaat
    26821 ccacttgccg atctttcaaa aataggtggc catgcttatc cataaaaccg taatctccgc
    26881 tatcccacca atcgccataa acattttcct ggaaccgtgc atcttccttg taataagtca
    26941 aagcccgtcc tttagacagt aattgtatat gaccatctgt gttttcaggt aggacattcc
    27001 caactgaatc tgtaatccga gcgcttgtca tgtcttccag tccaacgccc atatctcttg
    27061 catcacttgt tttcaaactc tctagtgtat gagccttaag aatcattggt ccgcattcac
    27121 tttggccata tacttgaaga aataccgcat cctgtgccat cgacgttcgc aaaaatgtct
    27181 tcattgtctg gttgtttata gcatcaaaag tggagtgata gtatcggata cccgaaaaag
    27241 cttctggatg ctccttagca gtaaaacgcc actgaacaaa gttattcgga tgggtctcta
    27301 gtgcaattgg ccggtgagct tccaacatat gaacaacttt tgaactttgt gcgtttgcta
    27361 aaggcatcat aggaaatccc atggacatca aagaagagat accaatattg aatctggaat
    27421 gcactggaga gatgtgaaaa gcgattaatt tcttttccga aatttttgtg aaaactgttt
    27481 tttgccattt cgtccgccat cccatagaat gagcagaatg acaaataagt ttagggatac
    27541 cagttgtacc agaagtatgg gtcatataag aaatttcatt tttgtctaaa aatgattgag
    27601 aaacagacaa agcaggttgt tccattaaat aaagaatagg aatacccgtg gtgctagtta
    27661 attttaatat cctaaagaat atctcaaagt tccttcaaaa cgtcgttgtg ctcgacttaa
    27721 tagaacactg taagccagta atatgctttc aaatctactc tctagccctt taacagaacg
    27781 tgagttgaaa ttttgacaac ctaatttttc taaggaagaa aaaacagtct caactgtttt
    27841 tctttgtttt ccaagtaagc tgtggtctag tttttctgat ttatccatgt ttttacgtgg
//
LOCUS       DACDIE010000033        27451 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.033, whole genome shotgun sequence.
ACCESSION   DACDIE010000033 DACDIE010000000
VERSION     DACDIE010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.033"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            147..479
                     /locus_tag="IV250_001574"
     CDS             147..479
                     /locus_tag="IV250_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8768863.1"
                     /translation="MDKYIHMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKIVNNLV
                     HLPLFFALLNYGLWLAIALVIVWRPYNNPGQRQIFAIIESIFFTDKNHYHALDPNRNY
                     HGTYKEDE"
     gene            480..1073
                     /locus_tag="IV250_001575"
     CDS             480..1073
                     /locus_tag="IV250_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8768864.1"
                     /translation="MRVKGQNSVDKKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            1095..2789
                     /locus_tag="IV250_001576"
     CDS             1095..2789
                     /locus_tag="IV250_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAP8768865.1"
                     /translation="MLDVIKTAFSTQSNQKVIDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLAFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNGATCSAVKSCGLQVV"
     gene            3477..5354
                     /gene="ltrA"
                     /locus_tag="IV250_001577"
     CDS             3477..5354
                     /gene="ltrA"
                     /locus_tag="IV250_001577"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP8768866.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            5485..5862
                     /locus_tag="IV250_001578"
                     /pseudo
     CDS             5485..5862
                     /locus_tag="IV250_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            5862..7067
                     /locus_tag="IV250_001579"
     CDS             5862..7067
                     /locus_tag="IV250_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP8768867.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDP
                     LPFLNSSKK"
     gene            7083..7727
                     /locus_tag="IV250_001580"
     CDS             7083..7727
                     /locus_tag="IV250_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8768868.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQAANTYKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPTDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            7738..8544
                     /locus_tag="IV250_001581"
     CDS             7738..8544
                     /locus_tag="IV250_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8768869.1"
                     /translation="MSKKKGERQRFKKRKSYLRWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
     gene            8566..8796
                     /locus_tag="IV250_001582"
     CDS             8566..8796
                     /locus_tag="IV250_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8768870.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            9342..9617
                     /locus_tag="IV250_001583"
     CDS             9342..9617
                     /locus_tag="IV250_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8768871.1"
                     /translation="MDLLIRNIEEKYINKIDKRCEELTVKTGKKWSRNQYLKVLIEND
                     FDHALLNYKKDQFDRLLEKFVDIQTYNTKTLEEYIATNNQLIGLLIE"
     gene            9659..10081
                     /locus_tag="IV250_001584"
     CDS             9659..10081
                     /locus_tag="IV250_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8768872.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAEERVE
                     GKIKSNQTKKYSSKLKKPVNESVHTRVDPFDPTKILKR"
     gene            10100..11692
                     /locus_tag="IV250_001585"
     CDS             10100..11692
                     /locus_tag="IV250_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8768873.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVISHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEIENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPEKRSEWNYGDENRTKLSPFTRGKLDELTKYVLTKEPGYQEYIQ
                     ISKALKEEAKSLYGESKRETKNAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVN
                     QMQSIYNERSFRTLKQLPTNEYKYSYQEFVKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            11704..12039
                     /locus_tag="IV250_001586"
     CDS             11704..12039
                     /locus_tag="IV250_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8768874.1"
                     /translation="MKQYLDQWKVIEGSLKEERIEQLPDCLEKEHLFQISEMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEINEEGINNFPCESI
                     EESISLTEC"
     gene            12186..12380
                     /locus_tag="IV250_001587"
     CDS             12186..12380
                     /locus_tag="IV250_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8768875.1"
                     /translation="MTQTTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGNI
                     LTLNLTTKMFVDAVENYPFV"
     gene            12412..14784
                     /locus_tag="IV250_001588"
     CDS             12412..14784
                     /locus_tag="IV250_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP8768876.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNNQFQKLFDHQKLTNGQ
                     PMNDKRNGRSWFNQQEKIENKIQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKEKDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSSEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            14845..16305
                     /locus_tag="IV250_001589"
     CDS             14845..16305
                     /locus_tag="IV250_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAP8768877.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFIPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            16316..18520
                     /gene="topB"
                     /locus_tag="IV250_001590"
     CDS             16316..18520
                     /gene="topB"
                     /locus_tag="IV250_001590"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP8768878.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQVGADQGKTFKRLWINSLEKEAIYQGFQQLRDAKETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKSEVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGMIRQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAESIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ENDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            18759..19352
                     /locus_tag="IV250_001591"
     CDS             18759..19352
                     /locus_tag="IV250_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8768879.1"
                     /translation="MKDKIQCIFDKHNGNLSLKDARSEGLSPMTLDRLAEKDKIEKVA
                     PGFYTLKDAFVDELYLAQSIYSRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     KLDQYAIQPHYTNKENFDLGVTETESFYGNKILVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMDILKSPKEMTLYMLPLY"
     gene            19368..20267
                     /locus_tag="IV250_001592"
     CDS             19368..20267
                     /locus_tag="IV250_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAP8768880.1"
                     /translation="MTPTQFKEKTRNFAAEKHLNPQIVQRYYMMEKLLECIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTANPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAYLENARVPFKLDITSGDSIIPAALMYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGKDNSRMKDFYDLYMIPKLERIDEKNLYKSVQGTFKE
                     RNNQSVFKHLYDQEMPQLVKDEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEKAERKQNFKRSQQMNQEMER"
     gene            20270..20755
                     /gene="ssb"
                     /locus_tag="IV250_001593"
     CDS             20270..20755
                     /gene="ssb"
                     /locus_tag="IV250_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP8768881.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFSLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRMHADQSSSSPSPTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            21121..21381
                     /locus_tag="IV250_001594"
     CDS             21121..21381
                     /locus_tag="IV250_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8768882.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            21822..22028
                     /locus_tag="IV250_001595"
     CDS             21822..22028
                     /locus_tag="IV250_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAP8768883.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            22028..22279
                     /locus_tag="IV250_001596"
     CDS             22028..22279
                     /locus_tag="IV250_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8768884.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            22294..22731
                     /locus_tag="IV250_001597"
     CDS             22294..22731
                     /locus_tag="IV250_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8768885.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFESIEKNGAFYTIESEEKDNE"
     gene            22724..23431
                     /locus_tag="IV250_001598"
     CDS             22724..23431
                     /locus_tag="IV250_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8768886.1"
                     /translation="MNKVVYQLTTEDVSQFLTSKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNHLDVYKRRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGK"
     gene            23812..23991
                     /locus_tag="IV250_001599"
     CDS             23812..23991
                     /locus_tag="IV250_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8768887.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            24332..24595
                     /locus_tag="IV250_001600"
     CDS             24332..24595
                     /locus_tag="IV250_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HAP8768888.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            24589..24939
                     /locus_tag="IV250_001601"
     CDS             24589..24939
                     /locus_tag="IV250_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP8768889.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(24963..25577)
                     /locus_tag="IV250_001602"
     CDS             complement(24963..25577)
                     /locus_tag="IV250_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8768890.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRL
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            25891..26313
                     /locus_tag="IV250_001603"
     CDS             25891..26313
                     /locus_tag="IV250_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8768891.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            26323..26526
                     /locus_tag="IV250_001604"
     CDS             26323..26526
                     /locus_tag="IV250_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8768892.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            26737..27321
                     /locus_tag="IV250_001605"
     CDS             26737..27321
                     /locus_tag="IV250_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8768893.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT        10170 a         4386 c         5626 g         7269 t
ORIGIN      
        1 caattgaatt atcaattacg acgaggaaaa aatcgttctc gtgatttagg aaatgaatta
       61 gagttgtaat atttagaaaa acgctgtgag aaatcacagt gttttttatt tctaaaaata
      121 caaaatataa ggggtatggt tatattatgg ataaatacat tcatatgaaa aatcctccaa
      181 gatttagtgg ggaatggaag tttttcgcct ttttcttttt agatctatta acgcttggtg
      241 gctgcttttt ttgggcgaaa atagtcaata atcttgtcca cttaccactc ttttttgcac
      301 tcttgaatta tggattatgg cttgcgatag cgttagtgat tgtttggcga ccctataata
      361 accctggtca acgccaaatt tttgcgatta tcgaatctat tttctttacg gataagaacc
      421 actatcatgc gttagatcct aaccgaaatt atcatggtac gtataaggag gatgaataaa
      481 tgcgagtcaa aggacaaaat tcagttgata agaaatccat tttgcagttt ggaaaaccag
      541 aagagcgctc tatcttggac ttatttccat ataaatcctg tacagaagag ggagtcttaa
      601 ttactagaga agatcatttt cagcgttatt atcgtgtcgt ttccactgac gtggagggat
      661 taaatgaaca agagaaacta gaacgaatga atcagttgac caccgtcatg cgtacgtatg
      721 tgccaacaat taagttagtt agtttgacca cagagacgga tctaacggaa caaatcgtgc
      781 aaaagcggca gttattagat aaaaatcgta tggaacagtc actaggaaga aatctacagc
      841 gattaagaaa atatgaaaag aaattagtag aggagatcca ggaattaaaa cgggcagaac
      901 aagaacgtcc tgacttaagc ttctttttcg ttattgaagg aaagacaatc aaggatttac
      961 aaactagaaa tcgtcagtta atgcgatcta gtggcatttt agggttaaaa ccactctaca
     1021 aaaaagaatt gattaagatt ctttatcgaa tgaacaacat gaatgacgaa taaaaaagat
     1081 ggaggaaatg attcatgcta gatgtgataa aaacagcttt ttcaacgcaa agtaatcaaa
     1141 aggtgattga tccattaaaa caacttggta atcgtgaaat agagatttta aaatcaaaag
     1201 gttatgattt agatttctta aaacaagtga tgccacaagg tgggttgaat tttgatgaag
     1261 aatatgtgac ctatggaaat ggttattcac gatgtgtgac agtttatcat tacacaaaaa
     1321 atcctactct tttttggttg gccaatctca tgaacaatcc tcatacagtg gccacactag
     1381 atgttagcac agatgaaaag caaaaagtga ttgatcaaat caaccgcgca ttagatgaat
     1441 taaatgatcg atcagataat gaacgaaaag cgactgaccg aaataaaagt gcggaagaat
     1501 acctggaact agcggaatat gcccgaaaat tgacttctaa tggggaaatt tctaaacaag
     1561 tcaaagtgag agtacatgtt tacggaaata cgatccaaga agtggatgaa cgggcgcagg
     1621 aactgatcga aaatctaaaa gggatggatt atcgtgcggt gacttatctg cataaaacca
     1681 aacgagattg gcaatcgatg tttaccatgt tcgatgaaca agctgaatgg tttggaaata
     1741 tcaaagggca atcacttcca tcgttaaaca ttggcggagg attcccgttc caccaccaaa
     1801 cgctaaaaga tccaggaggc acacaatatg gggaaaccat gacaggcggc ccatttgtct
     1861 ggaatgccac gtataaaaat agtgtacgtt tatctaccaa taccttgctt ctaggaatga
     1921 tgggagctgg taaatcgacc gttcttaaaa tgatcgcgga agcccattta gctgcaggag
     1981 actttatttg gggctttgaa aagggaaaag actttattcc tttcttaaag gaatataacg
     2041 gtattatggt gcgtttagac ggatctgacg gaatgatcaa tcctttagag atatttgcca
     2101 cacggacgta tgatgaagca tccagtttat atgacgatgg atcagtcaaa gatttgaaga
     2161 tcaatgaagc agcgtcctat caaacacact tagacaaagt ggtctatcag gtacagttag
     2221 tgagtcctca gctaaaagga acgatgaaag cagaatttaa gacctactta aatcgttttt
     2281 atgaagagta tggaacagta ccacgcggct ttacttcctc aaattcgcgt tcaaatactg
     2341 atacgcaagt cacagggaaa gatccagaag cttatccgac gttcaaagaa tttttagcct
     2401 ttttaggaca actggaacta ccaggggctt cccaagagaa aaagaatcgg aaagaagaaa
     2461 tggagtctat cgtggaatcc ttatgtgaga cttatggcat gatttttgat ggtcatagta
     2521 cgattcgaca ccttgaccaa caacagttgg tcttttttga cattgactct attacaggcc
     2581 tagatcgagg ggtacaacaa tgtcagatgt acatggccat gacgttgatt tggaatcagg
     2641 cattgattca aggacgaaga caacgacgct taattaaaga aaaacaaatg gaagaacaag
     2701 acttcaaacg cttcttggcg atgattgatg agtgccataa tggtgcgacc tgttctgcag
     2761 tgaaaagctg tgggttgcaa gtagtctaaa aactattgta ataactctta taacgagaag
     2821 tcaaatgagt gggtaacgcc acaaagggct tccactgata ctccgacatg caacaagtag
     2881 tttatgcaga ccaacgttgt gtgaagctcg gtgaagtcgg ctgaaggata ccgtaaggta
     2941 aaagaaatag tgaataccac gaaagttctc tagaggtaaa gggtgtatcc gataagtcgg
     3001 gtgtccaaaa aaacttaata tggtgagtat gtacaaacgt actgacgaac tagcgaatgt
     3061 actgtccgaa aggcatagct cctagaaatg ggagagacag tgagcaaaaa atcactcatt
     3121 gtgctaactt gtggttgagt aaaagtcgtt gttatgaaag accatgctgc gttacaggcg
     3181 taggagaagt tagggggcaa aagctagcat ctaagttaag gcatgtggat agatataagg
     3241 gaacagggga agccatcaac atggagagtg catactcgat gaagtggtag taagaaatat
     3301 gtgaatgaca aataacttac tttagtgatg gtgaaagtag agtcacgact gatgaaggtt
     3361 ctgtaatgga actggaggaa caggctcgat tcgtctgtaa aagtcatgaa acccgttcaa
     3421 ccatgggatt ctcgtatgac taaaaaggtc acgagccaat tggagcgtga taaaccatga
     3481 caaaagaaac aagtccaaaa gataggaagt tgcggcatgc agaatattat ggcatgacgg
     3541 aaatcttcga taagatatac tccaaaagtt tgaagggaga taatttcaag aatctaatgc
     3601 agattatcat ttctgataac aacattgtcc tagcatatcg taatatcaag agaaatcacg
     3661 gtagtgtgac aagaggagaa gatggagtaa cgattaaaga tattgaaaat ctcaaccgta
     3721 atgaattcgt agaaattgtt aggaagcgat tcgcacatta taaccctcgg aaagtaaggc
     3781 gtgtagaaat tcctaaatat aatggtaaat taagaccgtt gggtattcca tcaatatggg
     3841 atagaatcgc gcagcaatgc atcttacaag tattagaacc aatatgtgaa gcaaaattca
     3901 ataatcacag ctatggtttc cgcccaaata gatcagcaga acacgcaata gcagattgta
     3961 tggcccgaat gaatcaaagt cacatggaat atgtggtaga tgtagatatc aaaggtttct
     4021 tcgacgaagt caatcatact aaactcatga gacaaatctg gtctatgggt atacgagaca
     4081 aacagcttct cttaatcatt aggaaaatgt taagagcacc cgtagtactt ccaaatggga
     4141 agttgatata cccaacaaaa ggaacaccac aaggcggaat acttagccca ttacttgcga
     4201 acatcaatct caacgaattt gactggtgga tagcaaatca gtgggaggaa aaagatgcca
     4261 aagagctaaa acatcatttt aggaaaaatg gagcttggga taaaggtaat atctatcaca
     4321 agctcagaaa gagtacaact ctaaaagaaa tatacctagt gagatacgca gatgatttta
     4381 aaattttctg tagaaattat aatgaagcga aaaaagtttt ctatgcaact aaaatgtggt
     4441 tacaggagcg tctaaagtta ccaatatctg aagaaaagtc aaaaatcaca aatctttcaa
     4501 aagaagaaag tgaattttta ggctttacga tgaaattagt gaccaaaggt aacaaaaagg
     4561 ttgttaatac gcatataggt acaaaagcac aaagcatgat taaagtgaaa ctaaaaaaac
     4621 aaatcaaacg aattcaaaaa agtcctaata gcaaagatac ctttatctat attggagtct
     4681 ataacagtat ggttattggg attcataatt acttcaaaat tgcgactcat tgtagtgtag
     4741 caatgcgaaa aattgcaaga gaactcagac caagttttga aatacgacta cgagaacaag
     4801 gaatctcaaa agaaggacaa tacggaaagt acgaaggata caagccttac ctacggtcag
     4861 gacaaatacg atatataaga gggtttccaa tattaccagt agggtatatt caacacagac
     4921 atccgatgtg taaaaaaaga aatattaata aatacaccga agaaggtagg gaagccattc
     4981 ataagaagca acaaaccgta gaagaatgga agatatcgtg gttgtgtagc aatccagtgc
     5041 ttagtaagag agcaacgatt gaatttgcgg acaatcgaat ttcaaaattc atcgctcaaa
     5101 agggaagatg tgcagtaaca ggtgaggagc ttatcttatc tgaaatgcat tgtcatcata
     5161 ttatccctta tcatgaaagc aaaagtgata gctatgaaaa cctagtgatt gttactgagg
     5221 aggttcatcg ggtaatacac gctacacaaa gtgaaacgat tgaggaactt ctaaaatatc
     5281 taaaattaaa tccaaaacaa aaagaaaaac tcaatgagtt gagattaaaa gttggtaacg
     5341 aagaaatatc ttaataaata tttgtaggac gggaaaacag aatgtctttt atagacgagg
     5401 gaacgccgta tgcggtgaaa gtcgcacgta cggtgtggac tgggggaaaa gacggagata
     5461 atatcaaact cttacctatc agtatcgatt aatccagatt ttattgaaac ggtaaattat
     5521 gtcacaaact ttcaacgaga aatgcgtaaa gtagaagcaa tgactgtttt agctacacaa
     5581 tccccacaag aaatggtgcc agaaaatatg tcttctgata acttctcgaa actgaagaaa
     5641 gtctttgaat tttcttctac gaagatcttt atgcgtatgg atgaatctat tttacgtcat
     5701 atagaaggat tagtgggtaa atcaatgacg aactcagaat tagaatccat tcctcaacaa
     5761 aatcaaggag acgctgtgat caattttggc tcaaaagaaa ccattcgtgt tcattttagt
     5821 ccgaacaaac gtcaattaaa attgtttgat ggaggaaaat aatggaaaac caaaatgaaa
     5881 gtttaatcaa gcagtatgtt aagcgtcgtg caaagcggcg cttatttttg tggttgtttg
     5941 gtacttctgc aggtttgatt actattttga tcactgtgtt cgtgacccta tttctaatac
     6001 ttgcggcggg ctcgattgat aattcggatt cagattcaag cagtggtggc gaagctttta
     6061 caggagaata ttcggaaggc ttaccgattt ataaagaaat caaaggaaga ggcccttttt
     6121 ctgatgagat tgcgcaatat gccgtaggtg cagcagtgaa atataaatta ttaccaagtg
     6181 tgatcctttc gcaatacggt tacgaatcag cgtttggtac atcggcgtcc gcaagaaatg
     6241 atctcaacta ttttgggatt acttggtttg atggttgtct gtttcctaaa ggaactgcac
     6301 gaggaattgg tggcattgaa ggtggctggt acatgaagtt tcccaactca aaagctgcat
     6361 tttcctatta cggctttatg gtagccacac aatccaattt taatgcttgt gtaggaaata
     6421 aaagccctgg cgcaagctta ttgatcttag gacgaggcgg ttatgccgct gcaggaatta
     6481 cggaagatag tccgtattat acaggttgta tgtcaattat cacttctaat aaattaaccg
     6541 aatatgatga attcgccatc aaacattggg gtgaaggtgg caataacaac ggcacgatta
     6601 ctggtgaatg gacgaatcca ttccctggca gcagtttaga taaaagttcc ttcagcggtg
     6661 gtcaattatt tggaacgaat ccaggcggtg aattccgtcc gaacggcttc catgatggat
     6721 tagattttgg ttcagttgat catccaggaa gtgagattca tgcggttcat ggcggaaaag
     6781 tcgtgtacgt tggcaatcct ggtatttcag gtttgggcgc gtgtgtgatc gtgattaatt
     6841 acgatggctt gaatatggtg tatcaagaat ttgcaaatag tacaggaaat tctcgagtga
     6901 aagtaggcga tcaagtgaaa gttggtcaag taattgggat acgagataca gcacacttgc
     6961 atttaggatt tactcgaatg gattggcgtc aagcacaagg acatgcattc attgatgatg
     7021 gtacatggat cgatccgtta ccatttttga atagcagtaa aaaatagatc aggagaaaaa
     7081 atatggaaaa taaaacgtta aaaatcgtct taacagctgc aattagcgtt agtttaatcg
     7141 gcaatattgc gttgttttgg cagctgaaag aggaacaagc agcaaatacc tataaaattg
     7201 aagaagtggc taaaaacgcg tctgagaagc tcaaaaaaaa ttcctctgag gcacaggcga
     7261 agatcgacaa gttaaatcaa accatcaatg agaaaaacca agaaatcgat cagctgaagc
     7321 aacaagatgg cacaaaagca aaagaggaag taactgaagc acaaagaaag acggcagaag
     7381 agttcggtca attggccatt gataaatcat taagcagaaa tgaattaacg gaaaaactaa
     7441 aagatgtcgc aacccaagaa gtgatcgaca aattaagtcc tacagacgat gatcaccaac
     7501 atgatgatta cggaagttac tcctttacat taggtgacgt gcaaacttac gctcaaacaa
     7561 gcagtgtaaa agaggaacaa aattttgtcg tatttgttga ttatacaatc ggcaatccgc
     7621 aattcaaaga tgtcaaacca caaaaaataa aaggtggatt aactgtcact gaaaaacaag
     7681 tagatggtca atggaaagtc atagacttta gttatttcac tcgttaggag gaagaaaatg
     7741 agtaagaaaa aaggagaaag acaacgtttc aaaaaacgaa aaagttatct tagatggctg
     7801 ggaatcttta tgattctcgg ctttttgatt cttgctagtc cgatttatac gaatcagaag
     7861 gtagatatac cagatgaggt aagcacgaac gcctttttaa acacgaaaga gctgacaatg
     7921 gtttcaaaag aaaaaggagc gaaccagcaa ttagtttttg aatttgcgat cgatgatgaa
     7981 gatcaaagct tgttaaaaga actggctaat cttaattatc gcgtagaagt aaagacagct
     8041 aaaggggatt atcaagcaat acgagccaaa gtgattaagg tgtctgatga ttattttgca
     8101 gtgaagctaa cgcatgtacc agaagaatat atcgccatgc gtttaaccat tattcctgaa
     8161 aaaattgatc caaaagtgga tatgcaagaa ccacaagatc tgatttatta cgtgcacgaa
     8221 gataaggtta aagatcaagt gaaagatgaa gattatgaac aacacgcgat caattacaaa
     8281 gtgaaaggat acaagaaaga acaaaaagca gctcaaaaac aaatggaatc tttccaagca
     8341 accattgatt tgaatgaaga attagtgaag aagctaaaag atcaattgcc ttatcaagtc
     8401 gcagatgatc aggaaaatac tgaaaacaag attaatagct accagcaaga aattgaaaca
     8461 tcgaaaacgc aactgaaaga tcaagaagag atcattcaaa aacttcaaga aaaaattgat
     8521 cgtatgacaa aagaaaagat ttagaaggac aggtggagtg aaaaaatgaa gcgaacaaac
     8581 ttggctgaca aagatacttt cgctgagcag aaagtattga ttaaagccta tctcaaagtt
     8641 gtaaaagcta aagaaatatg tcgagaagtg aatttatcta acacaacaat atctgagttg
     8701 aagaatggtc gaaagaattt agatacttta aaattcagat catttgaagc tctatatgta
     8761 gctgctaaaa aatattttac agctgataac aaataaaaaa caggagtatt ttatgtgaaa
     8821 aaagtatata aaaaatgctt atgaatcagc tatatcacct taaccatttg ttaatgagat
     8881 gggagaaaac attaaccacg attctaacgc aaacatcaag cacataaaac atttaacact
     8941 cctcctttct ggctgaagat ttcaaccaaa caggaagaca gcgtatcaat tatagcatac
     9001 actttttctt aaattcattt ttcatatgac aaaatatttc cttctaatcg acaaagttag
     9061 aaggattttt tattgtctaa atacacaaat aaccttaatt tttaaagagc ataaggaaac
     9121 gtgatatcac tttctcacga aaggtgttag gtaggctgtg cctaccacgt ttctaagaaa
     9181 gataaatgat atcaaaattt tggatttgtg tgtagatttg tgggtaggat gaaagcgaat
     9241 gtgggcagta aaataagcgt ttgtgtgtag caaaatcaaa aaatgtgtag ggatatgtgt
     9301 agctacacat atccctacac ataataaatt tggaggaaaa tatggactta ttaattagaa
     9361 atattgaaga aaagtatatt aataaaattg acaaacgttg tgaagaactt acggtcaaga
     9421 ctggcaaaaa atggagtcga aatcaatacc taaaagtttt gatcgaaaat gattttgatc
     9481 acgctcttct gaattataaa aaagaccaat ttgatcggtt gttagaaaaa tttgtggata
     9541 ttcaaactta taatacgaaa actttagaag aatatattgc aacaaataat caattaattg
     9601 gtttgttaat tgaataataa aaatagtatt gtgaatttat aaaaggagga aatcaatttt
     9661 ggcaaaagca acaagaaaac atgtatatct ttacgatatg catatcgaag tacttgatga
     9721 aataatttct actcatgaag atatatctaa ttatagtgaa gcagtcagac atttgtgtat
     9781 gatgtatgat ccaaaaatcg aagaaaaaat aaatccaatt caaattaaat tgaatgcaat
     9841 gggaaaagaa atttctattt taaccgaaat ggtttcagag tttgcggatc ttcatttgaa
     9901 agatacagga atactaattg gtacaaattc aactgtttat caagaagcag aagaacgtgt
     9961 ggaaggaaaa attaaatcca atcaaaccaa aaaatattct tctaagttaa agaaaccagt
    10021 gaatgaatct gttcatacca gagtagatcc atttgatcca acgaagattc taaaaagata
    10081 aaagtagaag gagaacgtga tgaataaatc gcctgcagtt attcttactt gtcaatttac
    10141 cttgcccaat aaaaaatctt tttcaacata cattgattat atgacaagag aaaaagcact
    10201 ggaagaaatg gaacgacgta cgcccgaaga agaacaggaa ctgcaacgaa tcaagaatgc
    10261 tttagaggat ttttatattc ctgaaggaga aactttttcg gaaagtaaga cggataaaga
    10321 attcagtgaa acgtcgaatg aagctcaatc gttgatggaa catggaatca attttgacaa
    10381 gttagaggaa caagatttta ccaaatatct ttcttatatg acccgtcact atgcgttaga
    10441 acaaaagaaa gaactgacgc aaagggaaca aaaagaatac caacggttga accaagcaat
    10501 gaaaaaatat caaacagagc cacataaaaa agcttcagga aatcttcctg gagttttttc
    10561 tatggcttca gacgaagtga aaggaagtga tctgaaggag atcaaacaaa tcttccaaag
    10621 aggacaacaa aaaggctcaa tcatttatca agatgttatc tctcacgaca attcgtatct
    10681 tgaaaaaatg ggcttgtacg atcccaaaac agatacctta gatgaagaag gtttgaaagc
    10741 agctggaaga aaaatgatgg agacattatt cgagaaagaa caattgaatg aaacaggtta
    10801 ctggatggca acgattcacc gtaacactaa gcatatccat attcattttg cagttgttga
    10861 aaaagaaaat acgcgtaaac cgtatactga gattgaaaat ggtgtgagtt atatcgtacc
    10921 gaaggggaag agaagacaag caacattaga cgcaatgaaa accaattatg tacatgagtt
    10981 agaacgtttc tcttatgaaa aggatcgaat gatcggtatg gagaaacgaa atttattgac
    11041 acgaaaaagt gatttgcgta acaccttaac aaaagtagta aaagaaccag cacgttacga
    11101 tcgacacgcc atgaacttat taaatgaagt atataaaacc ctacctgaaa aacgttcgga
    11161 atggaattat ggagatgaaa atcgaaccaa gctttcacca tttacaaggg gaaaacttga
    11221 tgagttaacc aaatatgttt taacaaaaga acctggatat caagaatata tacagatttc
    11281 aaaagcattg aaagaagaag ccaaaagcct ttatggggaa agcaaaagag aaacaaagaa
    11341 tgcagaaaaa aacgccctct ttgatttaaa aaagcgcaca ggaaatgcga ttttaacaga
    11401 actgaagaga caggatagtc aaataaaacc attaatagaa agtgtcaatc agatgcaatc
    11461 tatttacaat gaacgatcct ttcgaacact caaacagttg ccaacaaatg aatacaagta
    11521 tagttatcaa gaattcgtaa agcgtaggaa acaattccaa caaagagttg tcacgcagcg
    11581 aagcttgcgt caattaaatc gaacaattaa tgcacatgaa gaaaaatacc acgcaagtaa
    11641 agcatatgaa gagcaacagc gaagaatcca aatggaacaa gaaagacaat aggaggaaga
    11701 gaaatgaaac aatatttaga tcaatggaaa gtcatagaag gatcactaaa agaagaacga
    11761 atcgaacaac ttcctgattg tttagaaaaa gagcatttgt ttcagataag cgaaatgttg
    11821 agaaacgaac agtttgatcc aaatcaattt ttagttgttg agtacccagc tactggtgtg
    11881 tattgttgta atcatgtgaa gggtgaaaaa tattttatta ttcaagaata cgaaggtaag
    11941 ttagcgcctt attatactac ttgggaaata aatgaagagg gaatcaataa tttcccatgt
    12001 gaaagcattg aagagtctat ttctctcacg gaatgttaaa taaatagaga cgtaaaagag
    12061 tcctgaacgt gattgtccag gactcttttt caatgaattg ttttccgcca attcactgag
    12121 tggtgcgatt caattttatt atatcaaaag aaagtgagat tccaaagtga agggaggaag
    12181 aagaaatgac tcagacaacg attggtgttg tggttgaaaa aagcagggat aatctcgttt
    12241 ttgtgacaga aattcaaaca ggtagagctt ttgtagtcac agacaaagct gcaaaagcgt
    12301 atcaaaatgg caatatttta acgttaaatc tgactacgaa gatgttcgtt gatgcagtag
    12361 aaaattatcc atttgtttag acaaacgata ttttacaagg aggaaacgga catggcagaa
    12421 gaaaaagaac cactagataa tagtcggttg ggaaagtcca aaagaaaatt agtacggttg
    12481 caaaacgaat taaataatca atttcaaaag ctatttgatc accaaaaatt gacaaatggg
    12541 caaccaatga atgacaaacg aaatggtcgt tcatggttta accagcaaga aaaaatagaa
    12601 aataaaatac aaagtttgag agaagagatc aagcaacaag aaaaacaggt agaaaaactt
    12661 gaaagacaag aagagctaaa ggaaatgggc tataacaagt acggcggact ggatatgacg
    12721 atcgataata ttccaagaat caaagaagaa atcgaacgct ttgaaaaagg tgaatcgact
    12781 ttttcagccg ccacgatccg aaaatatcaa cggaagttgg aaacattaga acagctgaaa
    12841 gaaaaatcag aaaaaggaaa agaaaatatc ctccctgaag tacaagcgat cattgacagc
    12901 ggacgagtga cgcaatggaa aaaaaatcca accatttatt ttttgaaagg ctatcgaaaa
    12961 gttgctttag aactgaatga aaacggggga tttgaggaat caaaaaaata ccaagccacg
    13021 tcggaagagg aaaaagcagc tgttcgtgaa ttacttgaag aggctgcacc aactgattct
    13081 ctgaaggaga aagaaaaaga tcatttctat cgagtagaat tcaacgaaaa tgatccagaa
    13141 aaaggattga aaagttatgg tggggagatc gtaacattag aattgatcga tcaacttaaa
    13201 acgttggata accaatacgc tgcagaagca ggttattata aattctattt tgaagaagtg
    13261 aagaacggag aagtagtaga tcagatccga atggatctag gggatggcgt caacagtaat
    13321 caccccatct atttagagtt agcaaaattg tgcgaacaag tacctgagaa ggaacaaaaa
    13381 caatcggtca aaaatgaacc tgtttcgaaa gaacaaatgc agtcgaaaga aacaaaaaca
    13441 aaacaaaagg gctatcaaaa acgaacggca gaagagatca aacaagaagt taaatccctt
    13501 tcgcaacagg cactggatgc ggtgaaaaag catacccatt cttctgaaga cgtcaaagaa
    13561 cttttgaact ttatgtctcg ttttccagaa cgatccttta gaaatcaatt attgattgag
    13621 gaacaatttc caggtgcaac ggcttgttta ggtcgtgccg cgttgaaaaa agaaggaatc
    13681 tatatcaaaa aaggggaaca agggaacaag atctttgtca gaaaaacggt caaaggattt
    13741 tatgagaaag gacgtggctt tgttcgagaa acagaagcta caccagaaga taaaaaacga
    13801 atcgaaagtg gtcaaatcga agtgatcaaa aaaccttact acacgattga aaaagtgttt
    13861 gatatcacac aaacacagtt aaaacctgaa gactatccga aaatctttcc gaaccgtgtc
    13921 tttgattttc aattggataa aaaaggacag tcagaacttc aagctgggat tcaagcggta
    13981 gccaacaata ttggaataca aattcgagat atgacagaaa gtgaagtcta tcaaagagaa
    14041 ttaggacaag cacgtggggc gtatgttcgt aacgaattta ctggcgaaga agaaatcgtc
    14101 atgaatactc gaaatactcc tacgcagcat ttagctacaa gtattcatga attagcccat
    14161 gcaagaatgc acaaattttc agaattagat accgcaacta aagaattgca ggcagaaatg
    14221 acttcttata ttgtgtgcaa acattttgga atggacacgt cggagaaagc tattccttat
    14281 attgcagcat ggacgaagaa tggtcagacg ttggatgata aggaagcggc ggaacgtggg
    14341 aaaatcatga atgatgtcag ccgtgtcgca aatgaattca ttcaaaccat ttctaatgaa
    14401 atcaatcaat acagagaaag agaaccagag atccaaccag cagaagtgaa gcaggagcat
    14461 aaaaatactg tagaaacggc agagaataag aatattgtgc ccgttggttc actttggatc
    14521 gacaagaaag acgggaaagt aatggaagaa gatacgggtc gaacgttaca tttgaaggat
    14581 gcggcgttta ttgaagatcc gcagcataat caagtgattt taaaaggaaa aagttatgga
    14641 aaagagattc aaacggcgtt accttcagaa gaatttgcgc taggaaatcc attccgattt
    14701 tcacttgaaa cagtgtcaga agtggccaaa cgaaaaaatg aaatctatta tgaacaacaa
    14761 ttgtcacaaa aagaacgcgt ataaaacaag taaaaaagac tatctgaaaa acggatagtc
    14821 ttttttcttt tgggaggttt gaaaatgacc gaaaaaaaaa tgagtcttat tgatcgatgt
    14881 aaacagatcg atatagtgga ttttgctcga aacaatggaa tggctgtagt caataaagga
    14941 cgagactatc gcttagaaga ccatgattca tttgtttttg atcgaagaaa acaacgtttt
    15001 tattggaaca gtcaaaatat tagtggcgac attattgaat tagctaaact ttttttcata
    15061 gacaaagaga tccaagactc taagcaacaa tttaaagcag ccactgattt tattctaaag
    15121 aatgaagaca aaacagaacg agtggaaaat cttcattttg aaacagaaaa gtacaaagat
    15181 caccccgttg attatcaacc actcacagaa aaaggaagga attatctgaa ggaagaacga
    15241 aaactaccag agtggctgat tgattatgca gaaaaagaag ggttgattgc cgaactaaag
    15301 ccaaaatatg aacggcaaaa tttcttagtt cgagacgatc gtttagatca tgcggtcgcc
    15361 tttttatgga aagatccaca gacaaaagag acagtagggg cgagttatca aggaacgttc
    15421 attgattatg aacgttttgg tgagagaggc acatataagc atatagataa aaattcgaca
    15481 gcgaatcatg gctttaacct aaagatcggt gaccctaagc agttaaaatt ttttgagagt
    15541 agtattgatt tacttagcta tgctgcactg aatcgtgatc agttaaacga tacgtggtta
    15601 gtctctatgg aagggttaaa acaccatgtg atcagtcatt attttggtga ggcagtatct
    15661 gaattgagaa agaaacaagc ctttcctcaa tccatcgaga tttgtgtgga taatgaccgt
    15721 gcaggacata ttttctacga aaaagaacaa ttaatgggcg cagtcgatcc atttacgaat
    15781 cagaaagtac gttgtgaacg aggaatcgct aatgattggc aagtgccaaa agaatacaaa
    15841 atcatttatg aagaagtcgc aaaagaagaa aaggtcacac cagaagctat tatggccata
    15901 cacaagacag aaaacaatct gcaattaacg aaccaactgg tttccgctca caaagtaaat
    15961 gcgttttttg gccagcagct ttctgttaac gattcaatag aagccattaa tttgaaagat
    16021 atttgtagag aagtcgcaaa agaattaaaa gtttgtgaac gcgtggatgg tacgtacgat
    16081 tttgatcgtt tttatcaaga aaaaggggat attaatgcgc agattctttt ctcttataaa
    16141 gcagaacaat actacaaagg ctataaaaat catgaacatg aatttatacc tgaagtaaaa
    16201 aaggactgga acgatcaatt gaagcatgag atccagcaac aagaaatcag aaagcaaaaa
    16261 cgtgccatgt tgtttcaaca aggcagacaa caagaaaggg agtgaaatcg tcatgatgaa
    16321 aaccgttatt ttagcagaaa agccgtcaca agcgaaagct tatgccgata gcttctctaa
    16381 agccactcga aaagacgggt attttgaaat ccaagatcga ttgtttcctg gtgaaactgt
    16441 gatcacgtat ggttttggtc atttagtcga attagattcg cccgatatgt acgatgaaaa
    16501 ttggaaacaa tggtcattag aacatttgcc tattttccct acacattatc actatcatgt
    16561 tcctaaagat aagaaaaagc aattcaaggt ggtgaaacag caacttcaat cggccgacac
    16621 cattattatt gcgacagata gcgatcgtga aggggaactg atcgcttgga cgatcattca
    16681 acaagtaggc gccgatcaag gaaaaacctt taaaagactt tggatcaact ccttagaaaa
    16741 agaagccatt tatcagggat tccagcagtt acgagacgca aaagagacct atccaaaatt
    16801 tgaagaagca caagcacgtc agatcgccga ttggttaatt gggatgaacg gtagcccact
    16861 gtatagttta ctcttgcagc aaaaaggcat accaggaagc ttttccttag gcagagtcca
    16921 aaccccaacg ctttacatga tttatcaatt gcaggaaaag attagaaatt tcaagaaaga
    16981 accttatttt gaaggaaaat ccgaagtaat cgctcaaaat ggcgcatttg atgcgaagct
    17041 tgatccgaat gaaacccaag cgacgcaaga agcatttgaa gattatttaa aagaaaaggg
    17101 ggttcaattg ggaaaacagc caggcatgat tcgccaagtt gaaaccgaga aaaaaagcgc
    17161 ggcaagtcca cgtctttttt ctctatccag ccttcagtct aaaatgaatc agctcatgaa
    17221 agctagtgcc aaagacactt tggaagccat gcaaggcttg tatgaaggga aatatctaag
    17281 ctatccacga acagacaccc cttatattac ggaaggggag tatgcgtatt tgttggatca
    17341 tttagatgaa tacaagcatt ttttaaaggc cgaatcaatc ccaaccccta ttcatacgcc
    17401 gaatagtcgg tatgtgaata acaagaaagt ccaggaacat tacgcgatca ttcctacaaa
    17461 aacggttatg acagcagcgg cttttgaaca actaagtcca ctgcagcagg ccatttatga
    17521 acaagtgtta aaaacaacgg tggccatgtt tgcggaaaaa tatacctatg aagaaacaac
    17581 gatcttgacg caagtccagc agcttcagtt aaaggcaatc ggaaaagtac cgatagatct
    17641 aggttggaag aagttatttg gcaaagagaa cgacggaaaa gagaaggaag aagagccgct
    17701 acttcctaaa gtgacaaaag gagaaatggt gacggtagat ctacaagtcc ttgaaaaaga
    17761 aaccaagcca ccacagcctt ataccgaagg tacgttgatc acggcgatga aaacagctgg
    17821 gaaaaccgtt gatagtgaag aagcgcaatc catattgaaa gaagtggaag gaatcggtac
    17881 agaagcgaca cgggcgaata ttattgaaac cttgaaacaa aaggaatata tcaaggtaga
    17941 gaaaaataag ctcgtggtga cgaataaagg gattttgctt tgccaagccg tggaaaaaga
    18001 gccgctgctt acgagtgccg aaatgaccgc caaatgggaa agctacttgt taaagatcgg
    18061 tgaacgaaaa ggaacacaaa ccacttttct agccaatatc caaaaatttg tctctcactt
    18121 attggaagtc gtaccagggc aaatccaatc cactgatttt ggatcgacgt tacaagaagt
    18181 gaaagcagcc agtgaaaagc aagaagccgc gcgtcattta ggaatttgtc caaaatgcca
    18241 ggaacaagaa gtcttactct atcaaaaggt ggcagcatgt acgtctgaag cctgtgattt
    18301 caaactatgg acaacgattg ccaaaaagaa acttacagcg acacagctga aagagatcat
    18361 tcaaaatagc cgaacaagcc aaccagtgaa agggttaaaa ggtcagaagg gttcgtttga
    18421 agcaacgatt gttttaaaag aagacttcac tacaggcttt gaattttctg aaaagaaaaa
    18481 aacgaacttc aagaaaagaa cacgacgcac tacaaaataa tggagggtta atcttatgcg
    18541 taatacaaca cacaaacaac aagaaatcga aaatcaaaaa gccaaaattc gttcagaaca
    18601 ggaaaaaatg cgtgccagca aacgaagact gcagcaattg aagaaccagc agcggattca
    18661 aaaagaaatc gaattagatc gataaattcc tttcttgtta atataaaact aacatgttat
    18721 agttgtgtta gttttatatt aactttaggg gaagagtaat gaaagacaaa atccaatgta
    18781 tttttgataa acataatggc aacctatcct tgaaggatgc tagaagtgaa ggattatcgc
    18841 ctatgacgtt agatcgatta gccgaaaagg ataaaataga aaaggtagca ccaggtttct
    18901 ataccttaaa ggatgcgttc gtggatgaat tgtatctcgc tcagagtatc tattccagag
    18961 ggattttttc tcatgagact gcgctcgatc tttttcaagt aggaacatat atccctaaaa
    19021 agatcaatat gatgtttcct aaaggataca atgtatcaaa ggagaaatta gatcagtatg
    19081 ccattcaacc gcattacact aataaagaaa attttgacct gggagtcaca gaaacagaaa
    19141 gcttttacgg caataaaatt ttagtctatg atttagagcg aacgttatgt gacatgtgga
    19201 atccaagata caaagcaagc gtagaggtaa aacaagaagc attaaaagaa tatatgacaa
    19261 gtaccagtag aaatacaaat aagctaagaa gatacatgga tattttaaaa tcaccaaaag
    19321 aaatgacttt gtatatgttg ccactttatt gagaggagta aagatgaatg acaccgacac
    19381 aatttaaaga gaaaacaaga aatttcgcag cagagaaaca tttaaaccca caaattgtcc
    19441 aaagatatta tatgatggaa aaattattag aatgtattgt tgaatcgcct tatcatgatg
    19501 attttgtatt aaaaggggga tttctgattg gatcaaaata cgggttagaa aaccgttcaa
    19561 ccgttgatat tgatacgact tatcgaaatt cgaaactaac aaaagaaaag ttagaaactg
    19621 ttttttctga attaacagct aatcctacaa aagatggaat tcgttttgaa ttaaagggat
    19681 taacagagac gagggaagcc gattattatc caggttttca agcaaagttt attgcttatc
    19741 ttgaaaatgc acgtgtacct tttaaattag atattacttc aggtgattct attattccag
    19801 cagcattgat gtatgaacac aaattaatgt ttgaagataa aacgattcat attccagctt
    19861 atcctacgga acaaattcta gctgaaaaat tacatgccac gtttagtttt ggaaaagata
    19921 attcgcgcat gaaagatttt tatgacctgt atatgattcc gaaattagaa cgtatcgatg
    19981 agaaaaattt gtataagagt gttcaaggaa cattcaagga aagaaataat caatcggtgt
    20041 ttaagcattt gtatgatcag gaaatgcctc aattggtgaa agatgagggc ttgagagaaa
    20101 agtggaaaag ataccaacaa gaaaactatt ttgccaaaga catttctttc gaatctacac
    20161 taacttccgt tgattccctg atgcaatcaa ttgtagagca agagaaggca gaacgaaaac
    20221 aaaattttaa gagatcacaa caaatgaatc aagaaatgga gagataaaga tgatcaataa
    20281 tgtaacttta gtagggcgat taactaaaga tccagattta cgttataccg caagtggcac
    20341 agcagtggcg actttttccc tagccgttaa tcgtaatttc acgaatcaaa acggcaatcg
    20401 agaagctgat tttatcaatt gcgtgatttg gcgcaaacca gcagaaacga tggcgacatt
    20461 agctaaaaaa ggcagcttga tcggcgttgt cggacgaatt caaacacgga cttatgacaa
    20521 ccagcaagga caacgtgtgt atgtgacaga agttgtggcc gataacttcc aactattaga
    20581 aagtaaagca gcgacagaaa gccgtatgca cgctgatcaa tcgtccagca gtccaagccc
    20641 taccaccttt gaacaacggg atacagcgac gcctaacaac aatggattga acgcttcaca
    20701 gaatccattt ggtggacaat caatcgatat tagcgatgat gatctaccgt tctaagtagc
    20761 attttctatt ggttgcattt gctacggttt atgttataat ttagacaaca aaaagaattt
    20821 tcaatctata accaaaaacc actagctggc gggctagtgg ctttctttta cgtttctatt
    20881 ataagaatta acgggtaggc ttactgtctt tcttggcaaa tttacaatca agccatttgc
    20941 agatatagtg cgcggctact tctgccatga ttgcaaaaac aaaatcaaaa agaattttca
    21001 aacctataac ctcctcccct tgccagatta ggccggggta gcagacagca agactattat
    21061 agcataattg aaatatccaa atgattggat ttttgctata attttagcga ggtgattgtg
    21121 atgaacaaat atgttggttt gcttgataaa atccgagtaa taaaaacgca accgttattg
    21181 gttcgcttta ctttacaaac gattcatgaa tcgattaatt gtgtagttgc agatattgaa
    21241 attatcgata aattgctaat tatggatgat ggaaaataca atattgccgt cacaggtcac
    21301 ttcaataaac gaaatcagct agttattgct agcatgtatg ttagaaatcc cgatcatttt
    21361 acgcgatcga tgggcatata aataaaagaa gagttgtagt cctaaatgag gattgcagct
    21421 ctttttgttt tacatagaaa caggaaaaga cctttttagg aagacacaag tgccttctca
    21481 attgaaaaac ctttctctca ctccaaccgc caccgccgtt cgagaagaaa ggaagtgaac
    21541 gctgcacgtt cactaagata tggtgtcttg caaagcaaga caaaatagac aaaacttcct
    21601 ctcaccaaaa ggttcgttcc tgttcgttat ctctttattg atttgccaga ccggcaacaa
    21661 gttgcctttt atcaccaaaa agcgacaagg acaagggtaa atggcacgct gcgcgccctt
    21721 gtccttgtcg cttttcgttg atttattctg gccaacaaat caataaagaa agaactaacg
    21781 aaaggaagta aaacctttcc taagaggagg cagaatcaaa catgcgaaag aaatttgatt
    21841 attggggtgt tcccttcagc gaattattcc caaactatca tgcaccacat actgttgaat
    21901 gcgattgtgg agaaagagcg aaatgtatca aaagctatcg tttgtatcaa tgtccaacat
    21961 gcgggaaaaa atataccttg tcttatggcg attacatttt gattgatgag aaaggaaaga
    22021 aacgctgatg aaaacacaac acaaattatt gactgtaaaa gaagtcgttc aaattttggt
    22081 ggacaaagaa gtcatttctg aaaaagtagc agaagcttta cttattccgc ggtactggac
    22141 gatagaaacc cctttagtag aggatgcacc gactatgcta tttgaagacc tagtgaagat
    22201 tgcaggatat gaactgcatt atcaagaaaa catggaaact tatgaaatcg aatattggtt
    22261 aaccaaatta gagaactaaa aaggagaaaa aatatggctc aaaattacta tgacgaattt
    22321 gtcaaattac ctttggataa aatggcacaa aaaatggagg atatgacgtt cctataccat
    22381 gaaacacgtg tacccaaaaa acattacaag gaaaaattgt cagtcgcagt ggaagaaatg
    22441 attgaatcag gtgtcgaaat gaatctcatt gcgacgtact atcgaacact ggaagaactg
    22501 aaaaaacaaa atggaaaatg gtttttccaa gccttactat gcttggaagt aggtgtcaaa
    22561 ccaagcacta tcaaaccttc cgaatatcaa gccttagaac tgacttatgc aaaattcgca
    22621 cagattaaga aagctaaagc aatggctagc gactggctag atacttttga aagtatcgaa
    22681 aaaaatggtg cattttatac gattgaatcg gaggagaaag ataatgaata aggtggttta
    22741 tcagctaaca acagaagatg tttcgcaatt tttaacaagc aaagaacact tgaatgaagt
    22801 aatcagacgg attcaaaaag actttagttg tatggaagaa atgaagtact ttattcaaag
    22861 agtggaagaa cagttgtata tggaaagcga aacaagtcaa acgaaggtca cagtcagcca
    22921 taatcattta gacgtgtata aacgtcgtcc tgaaacggta aaaaaaggaa cagagcttct
    22981 acgactagaa acagaaaaat acatcatgaa tgtgtaccta gaaaggagtg agctggtcga
    23041 aaactttgtc agctacacga tttgtaggaa acactattac tcagaatcag aatattattc
    23101 aattttcgag agcatggaat taatggaaga agaaatggaa gagctaaaca gcaaaaatgc
    23161 gacggtagac gattattacc ttgtatttac taggtttttg acgtgtggga cggaatctat
    23221 cgtgttaatg ggaatggatt atgaactcgc tgtacccgaa gcatttttga gggaatatgt
    23281 agaagaagta gataacccag gcgacgactg gtacacgact ttaactgatt ttttagacac
    23341 ctacacttac gacgatacag aagaaatcca aaatgaactt gaccgtaaac gaatgccata
    23401 tcaacgattt gaaaggaaaa aaggtaaata accatttata ccgaaaagtg aggatgtgtg
    23461 aaataagaac aaaaaacctt gaaattaagg gaatggttct tatttcgcac caatcgctcc
    23521 aaccgccccc gccgcttagg tggtgcgaaa taagcgggca aagcccgata acctaagaga
    23581 aagaaggaga tccatatgaa taaaatcatt cctctattgt tcgtactgtt tgttggcgag
    23641 atacttatgc gtcatgctgc agctgtacat ttaaacttcg tagtattctt aggaatactg
    23701 gtattttgtt tgatcttttg gcgatttgta ttagcccttg tgagtctact attcaagatg
    23761 attgcagcca tatttttcat agggttattt cgatgaaagg agagagtact aatgccaatt
    23821 gcaataggaa ataaacggtt acccgtgaca ttagatgaaa agagacaaaa agaattgcag
    23881 caactaaagc agaagtacgg aaaaagtgaa tctaagatta tgtgtattgc gttagatcta
    23941 ttgatcgcgc aagaaaaagc aggatttgat ataccagcac taaaaaagtg acgtcacttt
    24001 tcatttgaag aaacaaaagt gatagcactt aattgttcct actatttaga atattttcta
    24061 tgtccttttt attccatttt gtatcaataa ctatgaaatc tttaaagtat ccagaagaga
    24121 ttatttttag atcaattttt ttcttacctg attctaaaga ttttgttttc ttaaacatat
    24181 tatgatttcc tttcgactat tttcatataa atgttaacat agtttatgga ttcatggaat
    24241 gttaatctaa tttaatagaa aaggataatt aagtatatac gttttttctt atgtatgtta
    24301 tactatgtat atacagaaag aggtgtctac tatggaaatg ttacctataa aaaaatgggg
    24361 aaatagtaat ggccttagat tgccaaagta tattatggaa tatcttggaa ttcatacaga
    24421 agacaaagtg aagataatac aagaagaagt aaacggtcag aagcggctaa ttatcgaagc
    24481 ggctaatata gaaaatgaat taacaattga acagttattt gaaaattaca ctgatgaaag
    24541 aaatcatgtg acgattcaaa atttaggtga agcagtaggt aatgaaaaat ggtgagaaaa
    24601 cctagacaag gcgatattct attattgaat acagcacctc gatctggaca cgaacagaca
    24661 ggtaaaagac cttatattgt tttaagtcat gatattattg cggattatag taacgttgtt
    24721 acagttgctc caatttctag tacaactaga aactatccgc tatatgtaaa tataaactca
    24781 gaatatgaaa tgaagacaac aggaaaagta ttattagatc aactaacgac aattgattat
    24841 gaagccagag agtgtatttt tttggaaaaa gcgcatgata gtttagttga ggagttattg
    24901 atgaaagtta gaacagtttt tcaaaaagta agcaaataaa atagaaacag cagcatgatc
    24961 aactattcgt gattgaccat gctgtctttt tttattctat atacggtttg tctcgtaaca
    25021 ttgtagtctt ttgcaatctt agcgatcgca acaccttttt ttagatcctc aacgatgttt
    25081 ttataaacaa gacgacgttg gggatctttt gcattggcac tgtatagctt aggacgtcct
    25141 ttataaacac cgttcgcttt tgcaatctta atgccttgtg cctgacgtcg tttagattcg
    25201 gtacgttctt gttcagcgat catggccaaa atttgaatga tcaaatcttt aatgaattta
    25261 tccaggagag gattgccaat tgcttcggcc atgatcggta gacttgtgat gatcagctgc
    25321 acatttttct tcttcaagta attgactgaa gcgatgatct cgtcgtaatt tcttccaagt
    25381 cgatcaatag cttccacaat aaaaatatct tgatctcgga cgaaatccag tgcttcttga
    25441 aaaacaagtc gatgagtaat cgttgcgcca gattgtttct caatgaaaat tctctctgca
    25501 ccgaattttt tcatttcttc aatctgccgt tgttcatttt gatcgatgga tgagacacgt
    25561 atgtatgcaa ctctcaaaat ttttacctac tttctgcaac tatttaaaaa acaccctaat
    25621 catacacgag aaaaccagat ttttcaatgt cgcggtcgtg tctagttagg gtatacctta
    25681 tatagacaaa aactaatgat ggaggaaata gtatgtgtca cttttctttt taaatggcaa
    25741 tattttttgt aaataaatgt caatcaagaa aatttaaacg attatgacat taaaaatata
    25801 ttttatgaat tttctagtat ataacttgat ttatctgtta agatttatta agaaaacgtt
    25861 ttcttatttt tgatttcgag gtgatagaca atgaaaaata agtttatttt atctttaggt
    25921 attttaacta ttggaattag tgtaactgga actacaattc ctacatttgc tgatagtaca
    25981 aatgaaccaa ctactgtagt agataataac caagaattaa ctcctttaga taggaacgga
    26041 atagccattg gtaacccaac taacgactta actgttactg aaaattattt aatagtttct
    26101 ccattacttg ctcgagcagc ttctaaaacg atacgagtga caggaacgat gatttttgat
    26161 aaagttggtt ggccttctag acctcctcaa tcacggaatt ggaaagaaaa aagaaatggt
    26221 tattggtact atggaaaatt gtatttacaa agttataaat cagctggaaa caattggatt
    26281 gcgacttatt caggaacctt aactagaaag tagggatttt ttatgccaga atttgtgaac
    26341 acaccaaatt tttatcaaga tagcgatatt ctaagtaaaa ttaatttacc gaaagaagaa
    26401 tcagctttac aagttcagcg aactttacag gagacacaaa aaattcaaaa atcaatttct
    26461 aatctagaag attctgaaga aaaaagaaaa aaaagagacc aaaaaacata tcaaaaagaa
    26521 aaataatgat tttaaaaaaa gagtgtctag tcgacattct ttttttctaa ttgaaagtgt
    26581 tcataaaatc acccatttct ggacactttc tttttttgct ttatttttca aaaaaacaat
    26641 tcatttcatg ttcataaacc cataacaata tctatgttca gttaaactat atggataaag
    26701 ttatgttaca ataaaattaa tgaaggagtg atttttttga aaataggata cgcccgggta
    26761 tctactggtt tacaaaattt ggatttacaa gaagacagcc taaataaatt tggttgtgaa
    26821 aaaattttta ctgatcatat gagtggggca aaaagtaatc gaccaggatt agaaatggcc
    26881 attgattttg ttcgctctgg cgatacactt gtcgtttggc gcttagatcg tttaggtaga
    26941 aatatggaag atttgatttc aatcgtcaat cgactgaatg agcgtggtgt gagttttcat
    27001 agtattcaag aaaatattac gatggacaaa tcaagttcta caggacagct catgtttcat
    27061 ttgtttgcgg catttgcaga atttgagcgt aatttgatcc tggaacgatc cgcagcagga
    27121 cgagcagctg ctcgtgcaag aggacggttt ggtggtcgtc ctgaaaaatt gtctgaacaa
    27181 gatttggaat tactgaagac tttagtggat agcggtacac ctattaagac tattgcggaa
    27241 cggtggaacg tctcaagaac caccatttat cgttatttgg caaaaatgga ggagaaaaat
    27301 ggaaagacaa attctcatta gtttttgtca tttaatgctg aaaatttttg ccatttatct
    27361 agaaaagtga caatagtatg agaatttttt gtaagtgtct actgataact tattctcaaa
    27421 tgttgtaagg attttattca tttgttaata t
//
LOCUS       DACDIE010000034        27400 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.034, whole genome shotgun sequence.
ACCESSION   DACDIE010000034 DACDIE010000000
VERSION     DACDIE010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.034"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..197)
                     /locus_tag="IV250_001606"
     CDS             complement(<1..197)
                     /locus_tag="IV250_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP8768894.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGI"
     gene            complement(203..1066)
                     /locus_tag="IV250_001607"
     CDS             complement(203..1066)
                     /locus_tag="IV250_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8768895.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(1080..1976)
                     /locus_tag="IV250_001608"
     CDS             complement(1080..1976)
                     /locus_tag="IV250_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8768896.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(2151..3422)
                     /locus_tag="IV250_001609"
     CDS             complement(2151..3422)
                     /locus_tag="IV250_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8768897.1"
                     /translation="MKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(3412..4929)
                     /locus_tag="IV250_001610"
     CDS             complement(3412..4929)
                     /locus_tag="IV250_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP8768898.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            5127..6017
                     /locus_tag="IV250_001611"
     CDS             5127..6017
                     /locus_tag="IV250_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAP8768899.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(6090..6914)
                     /gene="nadE"
                     /locus_tag="IV250_001612"
     CDS             complement(6090..6914)
                     /gene="nadE"
                     /locus_tag="IV250_001612"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAP8768900.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(6926..8398)
                     /locus_tag="IV250_001613"
     CDS             complement(6926..8398)
                     /locus_tag="IV250_001613"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAP8768901.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(9035..9397)
                     /locus_tag="IV250_001614"
     CDS             complement(9035..9397)
                     /locus_tag="IV250_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP8768902.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            9620..10372
                     /locus_tag="IV250_001615"
     CDS             9620..10372
                     /locus_tag="IV250_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP8768903.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(10512..11435)
                     /locus_tag="IV250_001616"
     CDS             complement(10512..11435)
                     /locus_tag="IV250_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP8768904.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(11450..11722)
                     /locus_tag="IV250_001617"
     CDS             complement(11450..11722)
                     /locus_tag="IV250_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8768905.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
     gene            complement(12192..13817)
                     /gene="groL"
                     /locus_tag="IV250_001618"
     CDS             complement(12192..13817)
                     /gene="groL"
                     /locus_tag="IV250_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAP8768906.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(13868..14152)
                     /locus_tag="IV250_001619"
     CDS             complement(13868..14152)
                     /locus_tag="IV250_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAP8768907.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            14377..15039
                     /locus_tag="IV250_001620"
     CDS             14377..15039
                     /locus_tag="IV250_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP8768908.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            complement(15115..16407)
                     /locus_tag="IV250_001621"
     CDS             complement(15115..16407)
                     /locus_tag="IV250_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8768909.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            16577..17206
                     /locus_tag="IV250_001622"
     CDS             16577..17206
                     /locus_tag="IV250_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HAP8768910.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            complement(17309..18118)
                     /locus_tag="IV250_001623"
     CDS             complement(17309..18118)
                     /locus_tag="IV250_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP8768911.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(18173..19042)
                     /locus_tag="IV250_001624"
     CDS             complement(18173..19042)
                     /locus_tag="IV250_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8768912.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            complement(19043..20359)
                     /locus_tag="IV250_001625"
     CDS             complement(19043..20359)
                     /locus_tag="IV250_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8768913.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            complement(20356..22191)
                     /gene="walK"
                     /locus_tag="IV250_001626"
     CDS             complement(20356..22191)
                     /gene="walK"
                     /locus_tag="IV250_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAP8768914.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            complement(22196..22900)
                     /locus_tag="IV250_001627"
     CDS             complement(22196..22900)
                     /locus_tag="IV250_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8768915.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            complement(23090..23950)
                     /locus_tag="IV250_001628"
     CDS             complement(23090..23950)
                     /locus_tag="IV250_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP8768916.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            complement(23937..24506)
                     /locus_tag="IV250_001629"
     CDS             complement(23937..24506)
                     /locus_tag="IV250_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAP8768917.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            24887..24959
                     /locus_tag="IV250_001630"
     tRNA            24887..24959
                     /locus_tag="IV250_001630"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24920..24922,aa:Lys,seq:ctt)
     gene            complement(25455..25841)
                     /locus_tag="IV250_001631"
     CDS             complement(25455..25841)
                     /locus_tag="IV250_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAP8768918.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
     gene            complement(25838..26359)
                     /locus_tag="IV250_001632"
     CDS             complement(25838..26359)
                     /locus_tag="IV250_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAP8768919.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            complement(26361..27395)
                     /gene="sppA"
                     /locus_tag="IV250_001633"
     CDS             complement(26361..27395)
                     /gene="sppA"
                     /locus_tag="IV250_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAP8768920.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
BASE COUNT         8192 a         5696 c         4510 g         9002 t
ORIGIN      
        1 tttattcctt gaaagaaaaa ataaatgata tttcctgatc cctcaccttt ccattgcgcc
       61 attacataaa aaacagcaca tgtagcaggc acgatagtaa taacaggcaa acacacgatt
      121 acccagagaa tacctaataa gatgtaatca gaaaccacaa tcaactgtcc ataaaattta
      181 gtctcaacta ttttcatctt cctcactctt tcattccaga catagctgtt ccttgaacaa
      241 aatatttctg tgcaaaaata taaaccacta gtaatggcag cattgaaata accgttccag
      301 ccattacagg tccataaaaa gaaatgttct gccctgtaaa taaagctaac cctgctggta
      361 aagttctcat ttttgaacta cttgtcaata tcaatggata aagcaagtca ttccaactat
      421 tcatcagcgt gaaaatccct aatgataaaa gagtaggttt gctcaatggc aacatgattt
      481 tcaaaaaaat attaaactca cttaatccat caatgcgtgc tgcctcttcc aaagaatctg
      541 gtaaagtaat aaaaaaggaa cgcatcatga agataccaaa tgcagttgtc atacgaggaa
      601 tgatcaaacc tgcataagta tcaagcatgc caagcaaatt gacttcaata aatagaggaa
      661 tcatgaagac ttggaatggg atcatcattg tcagcaaaac gaaatagaac aaaactgatt
      721 ttcctttgaa acgcatacga gcaaatgcat accccgcaag agaatcaaag aatacagaag
      781 tgatcactgt ccctccagca aaaatcactg tatttttgat gtaatctaat aaagggatcg
      841 ttgtccaaac cttaatatag ttatctaggg tatagctatc tgcaaatatt ctaggcggaa
      901 atgagataat gtctttttcg tgtttaaaag aagaaataat cagccacaaa aaaggaaacg
      961 caataaccaa caatataact agcatactaa tcgttcgaat gattttttta ccactaactg
     1021 cttttcgact attcacagta actgtaatcg agctttctgg tgccatacag gtttccctcc
     1081 tatttcattt gtttttcttt ccgtgacatg tacacattag tggagatagc tatagttgca
     1141 acaatgataa acaaaaccga agacagtgct gatgcatagc ccaattcata aggtgctgta
     1201 aatcctcgac tgtaaatata ttgaacagct gtttctgtct taaattgtgg gccaccagcg
     1261 gttgcaacat atacttggtc aaacacttgc atcgaaccaa tcagattcgt taaaatacaa
     1321 aaacctaagg agggaacgat ctgaggtagc gtaatgttaa aaaactgctg tcgatttgtt
     1381 gcaccatcca tttgagcaga ttcatacaaa gagggagaga ttccctgaat agcagtcaag
     1441 agaatcgtca tagtaacacc aaaattcttc caaacagtca tgatggctac cgtcggcata
     1501 gcaagggttg gatcgcgaaa aaattgaggg atcggcaagc cgacttgtga aaacaagtaa
     1561 ggaatcgccc ctacattcgg atcaagcaac atagaaaaaa taatcgcaat tgacgtcaat
     1621 gaacaaataa ctggaatgta aaatgtcgaa cgacaaaaac gattgaatct ggtattttta
     1681 gataatgcta ctgctgctaa taaaccaacg ataatttgtg ttggcgtttc gagtacggtg
     1741 aaatataaag aatgaatcaa cgaattaact gctcgttcat cactaaatat acgtgtgaaa
     1801 ttttcaaaac ttacaaaatt tcttgatgta aagaaaatat tcaaatctgt aaaactaata
     1861 gcaaatgttc ctaaaagcgg tatgacagta aataataata aaactaaaaa agatggagtt
     1921 aaaaacaaat acgcagcacg atcaggacga ttccaatatt tggtgaatcc gttcatatag
     1981 tccacctcat ctttcatttt gataaaatat acaatcttgg tttaaacgaa tctgaataag
     2041 atatccgaac aatcgctgtg atttttgcaa taacacctat ctgcaaatac taggtcgatc
     2101 attcagatat ctgtatcttg ccaacagtct atcctgtata aagttctgta ctatttttgc
     2161 agtaattgat tgatggcatc atctgcatcg ttcatgagtg tctcaggatc ttctcctgct
     2221 aataattttt caatcagtgg attgatgata tcgttattga tcgcagagat ttgggtaaaa
     2281 cctggtaaat atggctctgc atagtccatt tgtttcgata attcagatac aataggatca
     2341 ttttttactt cttcatcttt tgctactgaa tccaagtagg caggaaaacc attttcgata
     2401 ctccattttt taccaatttc agttgtattc caatatttca aaaactcata gacagcttct
     2461 tttttattat tatctgtact tgccggaatg gcatatccta cgccatctaa aatagctgtt
     2521 tttttgtctg aatcactccc ttgtggtaag gtagtcacat tataatcaat ctcgtttgct
     2581 ttcaatccgt tatttaacca tggcccattg atttcaatgg ctagttgacc agcattcatt
     2641 aaattgtcca tttcggcgcc agaaatcgat tctggaccag tcttgtctac atggatcatt
     2701 ttttggatcg cttttaacgt tttaaggttc tcaggagacg caaattcaga cttagtataa
     2761 tcatcgttca ctaatttacc gccatttgct aacatccagt tatataacgg tgcagttcca
     2821 tccttattga agacaaaacc tgatacattt ttactactat cagttaattt tggtgccatt
     2881 tctgtcaatt cttcccaagt agtaggtggt tgttcagtat ctaatccagc ttgtttaaac
     2941 aaatcattat tccaatacat atacatgcct tgtacctgca tggggacaaa atactgttta
     3001 ccttctactt cacctaattc aatagccgtt tctttaaaat cagatttatc tactccatca
     3061 tattcccaga aatcgtctaa tggttgtacc gctccgtttt ctacatactg tgcaaaatct
     3121 ggataattta atgcaataaa gtcaggtgct tttttagaag aaatcgctgt aggaagtttt
     3181 tcattcaaat tggcccaagt catgatatcc atttcaattt tgatgttttt atcatttgtt
     3241 ttattaaatt cattaacgat ttcttgcaat atttcaccat cagatgcagt aaacccattc
     3301 caaaaagtca aagtcacttc tttatcagaa tcagattctg ctgaacttcc gccaccgcaa
     3361 gctcctaaaa tacctgttgc agctaccaac aaagagccaa ttaaccattt tttacctttc
     3421 attttttgtt cctccttcat ctgataaacg acatctgacc acattccaag atagcggttt
     3481 caataaacaa ttgattttcc catcttcagc ttttacattt gttgaatgac ttggttttac
     3541 ttgttcactt ttaggaagat tagttgcttt tgtatcaaat ccactcattt cagaaaaatc
     3601 aatcactccc tctaatgata attcagacaa gtgaacagtt agattcattt cttcttctcc
     3661 acggttaact gcaaatatga caagttcttt gtcatttatt acggctatac tttctaaata
     3721 cggcacttct tcaaaatctt ccgtcgcata gtttgctgaa cgaatttgtg ggcttaacac
     3781 ctctccttgt ccatacacag cagcttgcat aaatggataa tagatcgtct gtctccaagc
     3841 ttctccgttt gtttctgtcc tgattggtgc aataacgttt accaattgag caagacaagc
     3901 aatctttact ctatctgcat gtttcaataa agtgattaat agacagccta atagtaatgc
     3961 atcttcaaaa ttatagttat cttctagtaa aggaggagca atctgccaag gcgctgcttg
     4021 ctcatcttgt tccgctgaat gataccaaat attccattca tcaaaggaca ggttgatctg
     4081 cttcttacta tgtttcttcg ccttgactgt atcgcacatc gctgtcactt cagaaatgaa
     4141 ccgatccatg tctaaggaac gtgcaagata atgagaaaga tcattttttt ggttgccata
     4201 atattgatgc agagacaaat aatctatttg atcgtacgct tgatcaagaa ctgttaactc
     4261 ccaatcacca aatgtaggca tttgccgatt cgaacttccg cataaaacca cctcaattga
     4321 atcatctacg agtttcatcg ccttagccgt ttcctctgct aaccgtccat attcttccgc
     4381 tgttttatgt ccaatctgcc aaggaccgtc catttcattc cctaaacacc atgtcttaat
     4441 tccaaaaggg ctctcttgac cattcgaacg ccttaaatca ctccaataag ttcctttagg
     4501 aaaattacaa tattcgacta aattacgagc tgcatctact ccacgagtac ccagattgac
     4561 agccatattg atttcggccc ccacatgctg acaccagtta taaaattcat gaatcccaac
     4621 ttcattcgtt tcaattgtcc gccaagctaa atccaacctt ctaggtctat ttgctatggg
     4681 cccaatactg tcttcccaac gaaatcctga cacaaaattt ccaccaggat atctaatcat
     4741 gggaatgttc aattctttta ccaagtgaat gacgtcttgg cgaaatccac ttttattagc
     4801 tgatggatga tctggctgat aaatcccttc atatactgct cgtcccatat gttcaataaa
     4861 tgaactgtac aaccgcggat caactttgcc tacacgcttg acactactaa tcaacattgt
     4921 ggcttccata actgacctcc tttaatttat acatgaataa tacacctcgt tgaaagcgtt
     4981 gtcaataata taaatgttaa acttacttat tttttatcga ttttaattaa ccaaaatatt
     5041 aataatgatt aaaacaaata atttaatata acttttatta acaaatatgt taatatataa
     5101 gaaaaacctt ttaagaggtg atatatatgc aactacaaat aaacgaagaa tccttaccgg
     5161 tatatgaagc attagctagt aaaacaagga tcaagatcat ccagctactt tccaaaaaaa
     5221 agatgaatgt caaagatttg gcaaaagaac ttggagtgag cagtgctatc acgacgatgc
     5281 acgtgaaaaa gctagaggaa gcaaacatca tcaaaacaga aaaagtagga caacaaaaaa
     5341 tttcgagcct tcgtgtcgat aaaatcgata ttagttttcc agaaaaaata ttcaatgctt
     5401 ttgatacgaa agaaacatcg atacctattg gtcactatac aaattatgca attgaaccaa
     5461 catgcggttt agctacgatc catgatttta tcgggaaagt ggatgaacca cgctatttta
     5521 tggatcctcg aaggatggat gcccgcattc tctggtttac cagcggattt gtcgagtatc
     5581 aggcacctaa ttttttaaat acagaggata cattggagat gttggaagtt tctgtggaaa
     5641 tttcttctga atttcctttt tcaaatgaca attggccttc agatatcact ttcagcctaa
     5701 atggtgtgga gctaggaaca tggacgagcc ctggagattt tgctgatatc cgcggtaaat
     5761 atacgcctga ttggtatcca gataatctta atcaatacgg cttgctgaag acgatccgaa
     5821 ttaccaaaca cttgacgaat atgaatggag aacccttgtc aaacatcacg atcaacgaca
     5881 tccctaaaga gcaagacact tggcatttgc gaatcgaagt gaaagacgat gccaaacatg
     5941 tggggggctg tactttattt ggtaaaggat tcggtaatta cgatcaggat atcaaattaa
     6001 aagtttacta cagctgatat ttttcttttc tattttataa aacaaaaaca gccaatccct
     6061 atgaagggac ggctgttttt gctttattgt tatttccaga aatcatccaa aacggaaatt
     6121 ggcaaatgtc gtttatgttg tgtcttgttc caccatgctt cgattttttc ttgtgcttga
     6181 gcgggaactt cttttccttc tagataatca tcaatttcat tatatgtgac acctagtgcg
     6241 acttcatctg caatcattgg tttcccatct tcaagatctg cagtcggaat ttttgtgtat
     6301 aatttttccg gtgcattcaa tgcttgaagc aattgtttcc cttgtcgttt gtccaaacgg
     6361 aataatggca aaatgtcggc tccaccatca ccaaatttcg tgaagaatcc agtaatgttt
     6421 tctgcagcat gatctgtccc taatacggct cctgctcttt ctccagctac ggcatattgc
     6481 gtaatcatac gttgacgtgc tttgatattt cctttgttaa aatcgctgat tcccacacct
     6541 gcttcgctta atacttgtac ttgtgcatca acagctggtt tgatatttac tcgcagacta
     6601 acatcgggct ggataaagtc aagagcagct tttgcatctt cttcatctgc ttgttcacca
     6661 taaggaagac gaacagcgat aaattgatag ttttcatctt ttgtttcttc acgcatttct
     6721 tccatcgtta gttgcgccaa acggcctgct aaagtagaat cttggccgcc gctgatacct
     6781 agtacaaaag ttttaagaaa cggatatttt ttcaaatatg ctttcataaa gtcaatactt
     6841 ttacgtattt cttcttttgg atcaattgtt ggtttgacac ctaattcatt gataatttct
     6901 gtctgcaggc tcatttattc tcctcctatg cttctgtatg aagttctgct acgctttttt
     6961 tcattcgttc catgatagcc attttatggt tccagcattc agtagacaga tcgactgggt
     7021 atttttgcgg attcaagtct cgtttatact cttcccatag tgaatctaga ttttcttttg
     7081 tatattcttt gatttcgttt agtgcaggta attcgtaaac tctttttcct tcgataaaga
     7141 tatcttgtaa aactgggcga gctgtaaagt ctcgaacagt tttattgata aacgtatgca
     7201 ctggatggaa catgaagata gcttcttctt ctcgagggtc ttcatcccat aatgtcacgt
     7261 agtctccttc agatttccca tcaaaatttc gagtgatgcg ccacacttgt tttttccctg
     7321 gagtagttac tttttctgca ttgcttgata acttgatcgt atccatcatt tttccttcat
     7381 catcttcgat cgatactagc ttgaatactg caccaagggc tggctgatca taggctgtga
     7441 tcaattttgt cccaacaccc catacatcaa ttttagcttt ttgcatcttt aaattcaaaa
     7501 tcgttgcttc atccaagtca ttcgaagcat agatttttgc ttccgtaaat ccggctgcat
     7561 ctaattgttc ccgaacacgc ttagatatat aggccatatc tccactgtcg atgcgtacgc
     7621 cttggaagtt gattttgtca cctaattctt tcgccacgcg aatcgcactt ggcacacctg
     7681 atttcaacgt atcataagtg tcgactaaga acacgcagtc tttatgggtt ttagcgtagg
     7741 ccataaatgc ttcataatca tttccataag attggactaa cgaatgagca tgggtcccgc
     7801 tagctggtat cccaaatatc ttcccagcac gtacattgct tgttgcatct gctccgccga
     7861 tatatgccgc tctagttccc cagactgctg catccagttc ttgtgcccgt ctagttccaa
     7921 attccaatag tggatcttct ccgatgacag atttgatacg agctgctttt gtcgcgatca
     7981 acgtttggaa attgaccata ttcaacagtg ctgtttctac taattggcag tgcgctaaag
     8041 gcccttccac ttggatcaat ggttcatttg caaaaactag ttctccttca cgagcagaac
     8101 gaactgttgc tttaaattca aaattttgta aatactccaa aaaaccttca ggatagactt
     8161 ctaatccacg caagtaagca atatcggaat cattgaacgt cagattctct aaataattga
     8221 ctaaccgttc caatcctgca aaaaccgcgt atccgtggtt aaaaggcatt tctcgaaagt
     8281 aacattcaaa aacagcatgt aaatcagctc gacctaattc ccagtatgtc tgcatcatat
     8341 tgatttgata tagatccgta tgcagcgtca aactgtcgtc tgggtaagta ttattcatct
     8401 tgtattactc ctcttggcaa gttttcattt cctacccatt ataacaaaaa aaattaatag
     8461 gaaaagaaat cttccatttt ttctgaaaaa gcacatgttt tacggatatt tcttgctaaa
     8521 aatgaactcc atctgctaaa actatccttt cttattggta tttaacctaa acgagaaaga
     8581 atacttattt tattcaatgt atccgagaca cgaagaaatt agcgttttct atttttctta
     8641 cttgataaaa taagtagatt ttcctactac aatcgtagcc gttaagatac cgatgattga
     8701 acaaatataa ccaaatgtct ctacaacata atcaatggca aaagtttttg aagcaataat
     8761 acgtctttcc ttttttgctt cttatcccca aagaaacgga tataacttat tatgtattga
     8821 atttttttgg aaaggcgaat tatgacaaga tataccttgc gttgaaaaaa ctcgcggaaa
     8881 aaatatcaca cgtttttaaa aagatacaaa gtaagaaagc agctgtcaat agaatggaaa
     8941 caaaaatggc gagacatcaa tcagtctcgc cttgtttttt attgtataca ttagatatgg
     9001 agcaggttac cataaagatg attattagtc gttattaaat agaatccttc gtattttttt
     9061 gttctatcac tacaatatct ttatttggac gtttgaattg tattccggca cgttctagct
     9121 cctgtttcac tcctacatgg actttgacat cttctcgtgt catttttaaa acgtgatgaa
     9181 tcgcatagtc gatttcttgc cttgtgattt ttttcttcgt attgatcatc acgacttgct
     9241 ttttatcata tgtgtctacc aatagcagtt gtgttcgatc taacgaaaat aatttgtaat
     9301 aattaatagc cgtattccca aaaaagaatt ccactacttt aggataaaat gtcttaatat
     9361 ttaaattact attcacaggt gtttcgatta gcttcatctc attcctcttt tctatatagt
     9421 cgatttagat ccaatcttaa gttatgttac catctttcct agcattaagg aatcggttac
     9481 aattgaacct ttaaagacaa aaggatttac cttttatcac ttaatagtat aacataattt
     9541 catgagaaaa aaatacaatt ttttcttgac catctcgtta cgtaaaggat tatgcttaca
     9601 tttgtcagga ggaaaaataa tggagtacac aataaagaaa atgtcagaga tatcaggagt
     9661 cagtccgaga accttgcgct tttatgatga gattggactg ctaaaaccag ctaggatcaa
     9721 ttcttcaggt tatcggattt atggcaaaaa agaagtcgat cgtctgcaac acatcttatt
     9781 ttatcgaaca atggcattca agttggatga tatccaagag gtcttagaca atccatcttt
     9841 tgaccaccag aaagctttaa tcaagcatca gcaaatgttg ctggaaaaac gggcacaaat
     9901 cgataccctc ttgacaactg tccaacagac acttgatatg tacgaaggag gaagaaaaat
     9961 gagtgacaaa gaaaaatttg aagggtttaa acaacaaaag ttgaaagaaa atgaagaatc
    10021 ctatggacaa gagattcgac aaaaatacgg aaaaaaaaag gttacagcat ccaatgaaaa
    10081 atggatgaat atggataaag aaacgtatga agaaatgcaa actgttgaaa agcagatgtt
    10141 atttgatctc actctttact taaaagaacc agaagatgaa agcttagcag accgtatctt
    10201 ccaagctcat aagaagtggt tatcttacag ctggcctgaa tatcagccaa aagcgcataa
    10261 aggactagga atgatgtacg aagccgatga acggttcact gcatattatg acgagcgaag
    10321 tggtcatggt gcagcaaaag ctttaaatga aattatccaa aaaaaggcgt aatcttttac
    10381 atgatccaac aaaaaagtac acgtaaaatg acctaataac aaaacagcag acacccaagt
    10441 tagattttta tgctaacttt tggtgtctgc tccattcttt cacaagtttt ttcatcctac
    10501 tttaaatata ttcatcgttg aataaacgta taaggtattt tcactttatc ctttttttct
    10561 gtaagaaaca attgaaatgc catttctccg caagcttcca gatggtggtc gatggttggg
    10621 aaaccaagaa gctcgctgat cagcaaatta tcgcgtccaa tcaccactgg tgtcttatct
    10681 tgcagatagc tttgcaagat gcctgctgca accatatccg aatttgtaaa gacaccatca
    10741 actccaagct ctttaaaaaa gaagcctgct tgttttcctt tctctgcatc tatacaatcc
    10801 caaaaaacaa gtgattcatc aaaagatgga aaaagttgat gacatagctg caaagtgatt
    10861 tttgagttat aacttagcct gccacttcgc cccaacgtga tgcccaattg ctttatacct
    10921 tgctgcttca aataagtaag accttctgta attgattgtt ctcgatcaat aaagacacag
    10981 cttgcctgtt ttcccttgat ttcttcacaa aaaactagag gtccatattt ttgatatgcc
    11041 aataaatcat ctagtgtatt ggcacgagac gtaatgatca atccatcgaa agctttagct
    11101 gcaaattctt caagatacct tcgctctttt tctttctgat agcccgtcgg taaaagtgtc
    11161 actttgtaat cataatcaaa cgctgcttca atgatcccac tgatcaactg atcaaaatac
    11221 ggatgattgg tataaggcag aatcactcca atgcttttcg tttttccata acttaactgt
    11281 ctggcatttg cattagggat ataatcgact tctttcatga ccgcaagtat ttctttcctt
    11341 ttttcctcat ctacatatgg atgtccattg attacacgtg aaatagtaga aactgaataa
    11401 cctgtgagct ttgcgatatc tcgaatgttt gccactgctg cttcgccacc tatacaaaaa
    11461 gattttcttg taaatagtct accacagctg gaccgcattc aaaaaactga tacatttttc
    11521 cttcatgcga aaaactgatc cgctgcccgc cccaatatat atcttttgtc aaattttcga
    11581 tttcattcat tggtatttct tttactatat tcctattcaa cccttgaaaa ctgtacaacg
    11641 ttaatacgga tcccttgaac tcgacaaacg cataaaaata attgttcgtt gtgccatctg
    11701 ttatctctac ataagtattc attgcaagcc ctccttttga tactttgagt atagtgtatg
    11761 aaacgcgtct catagcaagg agtttgtttt gccaaagaaa agttttattc ctctttcatt
    11821 attaccaaag tctcttattt taaattgaat acattataat gatactgtaa atatatcttt
    11881 attattagtg aatggatatt tacttttaat agataaaata ttataactat taaaaataac
    11941 tattttcatt ataattacac acaaaaaaga gcgtgagaca aaagtaaaga acacttttgt
    12001 ctcacgctct aaacacgtat aaacagcgga agcagaagca actccttcgg aaataagctg
    12061 aaattcacaa aaatttgaaa agcaatttcc gtgaatttct tcttatttct tggagttaaa
    12121 cgcttctgtt ccagcctctt tttatctgtc tttcactaat gatcagttgt agataagcaa
    12181 gttgcttttt cttacatcat accgcccatc attgatggat ccattgcagg agctgctggc
    12241 gctgctggtt ctggtttatc tgcaacgact gcttctgtcg ttaataacaa ggcagaaacg
    12301 gaagcggcat tttgcaaggc agaacgtgtt actttagttg ggtctacgat acctgcttca
    12361 accatgttta cccattctcc agtagcagca ttgaaaccag tgcctagttc cacgttcttc
    12421 aatttatcaa caatcactga tccttcataa ccagcgtttt cagcgatttg acgaattggt
    12481 tcttctaaag cacgaacaac gatcttgata cctgttgcca cgtctccttc tgcttctaca
    12541 gcagaaactt tgctgataac gttcactaga gctgtaccac cacctgagac cattccttct
    12601 tctactgcgg cacgtgtagc attcaatgca tcttcaatgc gtaattttaa ttctttcaat
    12661 tctgtttcag tagctgcacc aactttaaca acagctacac cacctgctaa tttagctaag
    12721 cgttcttgta atttttcgcg gtcaaaatca gaagttgttt cagcaatttg gtttttgatc
    12781 aattgcacac gagcttcgat tgcttctttt tctccagaac cttctacgat cgttgtattg
    12841 tctttatcta caacaacttt agaagcattt cctaagtttt cgattgttgc atctttcaat
    12901 tctaaaccta gatcgtcagt aattactgta ccacctgtca aaatagcgat atcttcaagc
    12961 attgctttac gacgatcgcc gaaaccagga gcttttacag ctactacgtt gaatgttcca
    13021 cggattttgt tcaatacaag agttggcaat gcttcaccat ctacatcgtc agcaataatc
    13081 aacaatggac gtgattgttg caagatttgt tctaacaatg gcagaatatc ttggatgtta
    13141 gagatttttt tgtctgtgat caagatatat gggttttcta gaacagcttc cattttgtcg
    13201 ttatctgtta ccatatattg tgataagtaa ccacgatcaa actgcatacc ttctacaaca
    13261 tctagttctg tttcgatccc ttttgattct tcgattgtga tgacaccgtc gtttccaact
    13321 ttttccattg catcagcaat caaatgtcca actttatcag aacccgaaga aacagctgct
    13381 acttgtgcaa tggcttcttt tgaatcaaca acagttgaga tattatgcaa ttcttcgact
    13441 gctgcttttg ttgccaattc gatcccgcga cgaatgccta gaggattagc acctgcagtt
    13501 acgtttttta aaccttcgcg aacgattgct tgtgtcagaa cagttgctgt cgttgtaccg
    13561 tctcctgcaa tatcatttgt ttttgatgct acttctgata caagttttgc acccatgttt
    13621 tcaaaatgat cttctaattc gatttctttt gcgatcgtca caccatcatt tgtgatcaat
    13681 ggagagccgt aagatttttc tagtacgaca tttcggcctt taggtcctaa tgttactttc
    13741 actgtatctg ctaatttatc taccccgcgt aacatagctg cgcgtgcgtc ttctgcaaat
    13801 ttcaattctt ttgccatgat tgattcacct cattgattat ttttgattat ttttttatct
    13861 tccaaattta ctcaacaatc gccatgatat cttttcctgc gacgatcaga tattcttttc
    13921 cttcgtattt tacttctgta ccggcgtatt tttcgaacat aacttggtcg cctgctttta
    13981 cagcggcagg aactttttca ccgttttcta acaaacggcc ttcaccgact gcaacaactg
    14041 tcccagtttg gggtttctct ttagcagcag aagctaaaac gattcctccg acagtttttt
    14101 cttcttcttt tgcgacttcg ataatgacgc gatcacctaa tggttttaac acgataaatc
    14161 cctccgataa ctcttttttt aatctgttag cactcttaac cttcgagtgc taacctacat
    14221 ttttaataat actcattctc cctctttttt gcaagctttt aaatcaaaga aaagaaattt
    14281 tattctgaca gctgcttatt gaaattatga tataattgaa atcaatcgat ataagcttgc
    14341 cttgcatgct tgttatccct taaaaggagg aattacatgt ctttaaaaaa atatagcttc
    14401 ctcagcattt tttgctatgg tctaattttt atctcaccct tgatcctagc ttcgatagga
    14461 cttgtaaaat ctacgtctga attgatcaca gcaactgcag tcatgtatat actaggcgcc
    14521 gttgtcctag ctatttttta ttttaagcaa cgtgaaccct tagcgatcga atcagcggta
    14581 aaaaaagctt ctgctccaaa gattgttatt tatggattgg ttggaatctt tgtcgcattg
    14641 attctgcaaa gcatcgctgt catgttagaa tctgtattgt ttggcaaagc aaccccttcc
    14701 gagaatacac aaaatatcat ccagatgatc atggaagcac ccgcttttat tttggcgaca
    14761 actgttgctg gacccatcat ggaagagttc gtattccgcc gcagtatact ggggatcatc
    14821 agtcgatatt caaatttttg ggtcggagca gtgatcagct ctttgctgtt tgcgtttgct
    14881 cataatgatg gtcatctgct aatctacttt ttcctaggtt tcttcttcag cctagagtac
    14941 aaagcaactg gtcggatctg gacatcgatg atcacacacg taggaatgaa cacattagtt
    15001 gttcttgttc aattagctgt ccaaaaaggg ctagtataaa catgactata aatactcctg
    15061 tcatcctaga ttggtgattt tcccaatcta aagcgacagg agtatttttg ttttttagtc
    15121 gttcatcttt tctgctgctc catcattttt tgtctgtctt tcctttttgc agtttgcaga
    15181 atctattttt ttcaaaaatg ggaaataaat gaaaaaacta attgacaaca cgataccttg
    15241 taaaatcgct gcaggcactc cgcctactaa gaaacccgag aggatcggcg gtgttgtcca
    15301 agggacttgg actgctgtaa ataaaggaac gaatccaaaa tataaagcgg aataggtaat
    15361 caatcctgaa acgaccggtg cgaaaataaa aggaacagcc atcatcgggt tcatcacgat
    15421 cggtgtggaa aaaataattg attcattgat gttgaaaaag gctggtaaaa gagcaagatt
    15481 tcctaattct ctgtatttct tcgatttcgc aaaaaccgtc ataaaaaata cagcccctaa
    15541 cgtcaatcca gcacccgtaa ccgtcatgaa ctgatcgaga aattgctgag taacgatatg
    15601 tccgccattt gccaccgtca actcttgtcc tgaggctaag atttctgcat ttgctaatga
    15661 attcgcttgc aagacgggac taaccaaccc attgaccatc gcagatccat gaagaccaaa
    15721 taaccataaa aacggaacaa aaaaacctaa taaaagcaca ccaaacaacg tgtctatgaa
    15781 cgattgaatg ggaagctgga catatgtcca tactccttga acaacagtca ttctgaacaa
    15841 atgttgaaaa accgctgaac cgataagcgt aaaagtgact ataacaatga ttggaatcaa
    15901 tgaagtaaaa gactgccaaa cattttcagg tatcttctct ctttgcttcc accctttcgc
    15961 aagtcttact aaacgtgcat agccccatcc gatgatgagc ccagttgtaa ctgctcctag
    16021 tatcccttca gcacctgtcc agacagtatc tatctgagta tttagtggct gaataagcag
    16081 tagataagca gacaaggctg ttatgccgac tggtaagcga tcgatgtctt cttcttttgc
    16141 ataagcgtat gcaatggcaa aaacgatcca aaaggctatc acatcaaatg tagcctggta
    16201 tacttgtgta aaatacatag aaaatcctgt tttgtctatc cactgagcga tgtaatccac
    16261 aggaaaatga gcaaataaga aaaacagcga gccaacaata attattggga tcgtgtaagt
    16321 cattccatta cgcaaagcag ttaagggtct aagattcaca acagatgtta tcattggtaa
    16381 aaaggcacga tctaaccact ctttcatttc ttatctcctt caacttctat ttttatcaat
    16441 cagttttctt aatttaacta tacccaatga tcacgaacca tgaaaggaat gcgcactgag
    16501 cttttcaaaa atcggattct ttgacgaata ttcagcaact gcttacaatg gacaggaata
    16561 aaaaaggagg ttttttatga aaattgcact cattggagct actggccatg caggttcatt
    16621 tattttagac gaagcattgt ccagagattt agacgtgacg gcaatcgtgc gtcatccaga
    16681 aaaattgcca tcagatgttc cgtttataca aaaggattta tttgagttga caactactga
    16741 tcttgcatct tttgatgttg tcgttgatgc tttcaacgct cccaaaggaa aagaagaaat
    16801 gcatcaaact tctttgtccc atttgattga gatgcttgaa ggaaccgata cacgcttaat
    16861 tgtcgttggg ggtgcctctt cgttattctt agatacagaa aagacgactc gtatgattga
    16921 ccaagtacct aaaaatgcac ccttctatcc aacagctttc aatatgtacg aaggattgtt
    16981 aaaattgaaa caatctaaaa atctacattg gacttatatc agtccagctt ctttatttga
    17041 tccacatggc aaacgtacag gtacttattt gttaagtgat gattatttac aaagaaacgc
    17101 tgctagcgac agtacgatca gcatggcaga ttacgcgatt gcattagtag atgagatact
    17161 ggatgagaaa catgaaaagc agcacatcag tgtagtcagt cgataatacg gttatttttt
    17221 taatatgcta aaaaaataaa aatagaagat aaaaccaacc ctgattgatt ttatcttctg
    17281 ctttttatat ttttattgat tgaactgttt aaatcgaaaa tagctcagtt gcttcatctg
    17341 gatctgtatc atagatttca aaatcatgtc caacgccaaa atctttttct ttcaaaacat
    17401 tttccattgt taaatgagca agttctttca tattgttttc cttgctaaga tggccaagat
    17461 agattcgttt ggtccgatta ccgatcacat cagtcatgac aagtgcaccg tcttcgttgg
    17521 aaagatgtcc tctatctcct aaaatacgct gttttaaatt ccacggataa ggtcccatgc
    17581 gtaacatctc aagatcatga ttactttcca tcaaataacc atcggcattt tcaatgacac
    17641 ctcgcatata atcactgcaa tatccggtgt ccgtcaacat gacgaatgac cgattttctt
    17701 tatggaaacg ataaaattgc ggagccgctg cgtcatggga gacgccgaaa ctctcaatat
    17761 cgatatcgcc gaatgttctg actttcccca tctcaaatag atgtttttgt tccactggta
    17821 catttccaat cagtggttcc atcgcttccc aagtctgctg attggcaaaa acatccaaat
    17881 gatacttacg agccaagaca cccacgccat gtatatgatc tcgatgttca tgcgtcacaa
    17941 gtattccatc taattggtcc ggtgtccgat caatttgagc catcaaggaa gtgatttttt
    18001 taccgctaag tcctgcatcg accaaaagcc gttttttttc actttcgata taaagtgaat
    18061 tacccgaact tccacttgct aggatactga ttttaaaagc tggtttttct tcagccattt
    18121 gactcctact tcccttctta ctttgtaaac ttttttcatt atactcgctt ttttattgat
    18181 cttccacttt tggtacagta ttgtttgtca cgatcgtatt gctgaaagcg ttgacgctct
    18241 caatttggac cactttatcg cttgtttcga tcgctacatg ccaaacagga acatacacat
    18301 ttttttcccg gactttcaga atacaggaat aagctagctg acgccataaa atttttgatc
    18361 ctcttggtat tttgttattg atgtacaatg tattgattgc ttcctcttcc gtatacagtt
    18421 ccgtcttttc gcgcagctgc tcgatatcag ataaatgcgt ctgcatgtat ttcgtgatgc
    18481 tataatcatt atcgtttttt tctaaagaaa gaacgatttt tgctgtatca tcattgaatg
    18541 ggatgccttc atactcttgg cttgcttgga tttctagtgg ttcttcttct aaatgagaaa
    18601 agtttttcgt gtacacataa tcattaccga ataaaatatt gtctttgtta gataaaaaat
    18661 cttccaagga actactaatt ttatctggat cgatcacgtt cttttcagca tctgaactgg
    18721 ataaagattt cgtcaaaaca tcaccgtcta aaaacacacc acttgccagc aagccgcttt
    18781 taccagcctt gcgataattc gctaatgaag tgctaagatt ggtctgctcc gcgctaagat
    18841 aatagccttg cttgttctca gacgaaatct tccccgtgta agtgatatcg tcgctagcca
    18901 gacgttgttg gattgattca gtctgattag agcgaatgac attgttttct tggtataccg
    18961 cgtcatgata actgctgaat aaaaacacat tcaaccctaa aaaagcgacg aaaaatatcc
    19021 attcgatttt tttgaaatcc aattaattcg cctccaatcc aggtaatttt tccagaaggt
    19081 cgttagctga ataccagtta tctttatatt tcacatacca ttcaggtaag agatcaacga
    19141 cacgattcgt ttctttgaca ttttgccatg tatagccaac gatcactgaa ccaatcttgt
    19201 tgctatctgc tcccttagct tttaactgct ggatgatttc attcgtactt gcaagttcta
    19261 cttcttcgtc tgaaggtatc ggcacttgaa tcgtattcaa actggtttgg atattcactt
    19321 gcacagaacc attcgtttca ccgccaactt ccacaccaat ctgtcctctg ctgtctgtcc
    19381 caaaaaccgg gaagccttcc acgaatatcc gataatcgat cgtattgtca ctgcggtcaa
    19441 aatatctgag gttccctaaa gaactgccta atttgctaat ataggggaaa ctttcagaaa
    19501 atacactagc attttctttt ttctctggca gctctccacg aaaatcaact acttgcgttt
    19561 ccgaatggat agacattgtt tcattcccac cgtaaaaaat caattcattg ctttcatcat
    19621 tatttttcac gctatctggt ttctggaaaa aagcatttct aaaaagtgta tagggttgct
    19681 gtgacaggat ataactatac ttcttcagtt tgatcgaatg tttcgtagca tattgcgtgt
    19741 cgatcgtcga tggttctgtt gtcatttctg tccattttgt gtcttttgct gttaattctt
    19801 tattgatatc tgtccaattc aacgaaatat ttgcttcata tacgagatgg ttcgaataat
    19861 tcaaaaatcg gattttttta tttccttgat cgaattgtat tctagtaaaa tacatatttt
    19921 cgctttcttt tgccaagttc gacacatcta aatctaaatc aaatgcttcc ttgtattctc
    19981 ttagcaaaaa aggcgcatta taggccaact caaaaccaga gttcagattt ttcacctgtt
    20041 caaactgtga tttgtctcct gaaactactt cagaaagctt tccaaatctt gctttatcga
    20101 tgatcaattg caatcggtac acgagatttt cactgttggt ttcttggatc attcccgaat
    20161 ggtgccatgt aacatgaagc ggaagaaaga cttcggaagt ttttcgattg ctttgagact
    20221 cgatcatttg agtgtttgtc tcatctacgt tgattgaact ttttcctgct gggctcagcc
    20281 agattgcata agagaagtat atgcttaacg caaccatcaa aatgagtcct atacgaacga
    20341 ctttttcaga gattctcatt cccaccattc ctcctcataa ggttcatatg gcagcgagat
    20401 ataaaaagtc gacccttttc cttccttgct ctctgcccaa atagcgcctc gatgcgcctt
    20461 aatcacttct ctagaaatag ctaatccaag tcctgtccca ccttgtgctc ttgcacgtgc
    20521 tttatctact cgatagaacc gctcgaacac tcggttcaga tcttttttag gaatccccaa
    20581 tccttgatcg gtgatactta aaatcacgtt attatgcgtt tctaataagc gacaagtaat
    20641 cgttccccca tctggtgaat acttgatcgc gttattcatg atattatcga ctacttggat
    20701 gattttatct gtatctactt cgacccaaag atctctttgc gtaaattcac gacggatctt
    20761 ataatttttt tcctgatttc ccaccatcat atcaaaccgg tcaagaacaa aattcactaa
    20821 ctcgttgaaa ttgacatatt ctaattgaag ctgggtatta cctgtatcca tgcgagatag
    20881 atttaagagg tcattgatca ttcgaatcat tcggtctgtt tcatctagcg ttaccttcaa
    20941 aaaatttggt gctacttcct cgtctttcca cgcaccttcg ctcaatgctt cgatataact
    21001 acgcatactt gtcaacggtg tacgaagttc atgggaaaca ttagagacga attcacgacg
    21061 ttcccgttct gttttttctt gttctgtcac atcatgcaag acagctacta atccgctgat
    21121 aaaacctgat tcgcgacgga tcatagaaaa gtctactcgc aagatcatct gatcactttc
    21181 caatgtactg gttgaacggt caagtaccat ttcgtttgga tcttctaata atttccgcaa
    21241 cgtatattct tcttcgatat ctaaaagtgt caaaatcgat tgtccgattg cttcctcgtt
    21301 tttgatatct agcaaggaaa cagccatatc attgatcgta atgactttac ccctgcgatc
    21361 tgtcgcaatg acgccgtctg tcatatggga tagcacgcta tccaaacgat tgcgttcagc
    21421 ttccattgta tcttgagctt cttcaatacg ttcagctaac tgattgaagg tatcagccag
    21481 ctgtccaagt tcatcttgtc catgcacttt gactttccga ctgtagtctc ctctagcgat
    21541 tcgaatggct tgttctctca tttcaccgat cggctgtgta atcgaacgag caactaaaac
    21601 agatacgatc attgaaataa atgccgcaat tagtgaggca gtaaagaaaa tgactgctgt
    21661 gttattgatt tccgaatatt tttgttcgat attacttttg acgtaaagag ccccgataac
    21721 ggcatcgccg gtcggggaca ggatcggttg aacattgata ttcacccgtt tatcgtcgtt
    21781 atctaaagca acataacgtt tagtcgtgaa atcattgata tcaacataat catttttctt
    21841 tcccacagcg ctttgttcat tgacatcggt tgttcctcgg atgatccctt tttcgtcaac
    21901 gacccgtatt tcgatgatat cgctagtcgc actgttttca ataaatcttt tgatattcgc
    21961 atttacctct tcatcatctg cattatcttt tcgattcaat tccgggctga ttgttgttgc
    22021 taatgattcc accgtttgat tcatatcttt aataaatgta ttgatcgtgg aacgttctaa
    22081 accacgtata aagtaggcac caataatttc gatcgagatc aataaaatca gaataaaggt
    22141 aatagcgatc ttaaaattga ctgatcgaaa aaagcggact tttcttttca tccaattact
    22201 cctgttctgg gttacgcaaa tagtaaccga caccacgacg tgtcactaaa taatttggat
    22261 ggcttggatt atcttcgatc ttttcacgca aacgacgtac agttacatcc actgttcgta
    22321 catctccaaa ataatcataa ccccaaaccg tttgtaataa atgttctctt gtcatcactt
    22381 gtccaaggtg cttcgccaag taaaagagta gttcgaattc acgatgtgtt aactcgattg
    22441 tttctcctcg tttcgtcacc atgtaagctt ctggatggat cgtcaaatca ccaatcgtca
    22501 attccgcagg tgctgcctct tctggttcct tcgcagccgt ggcaccgcga cgaagatttg
    22561 ctttgacacg tgcaactaat tctcggttgg aaaatggctt ggtcacgtaa tcatcggcgc
    22621 ctaactctaa gcctaggact ttatcaattt cagaatcttt ggctgttacc ataatgatcg
    22681 gcatatcata agttttgcgt acctcacgag caacttctaa gccgtccatt ttaggtagca
    22741 tcaaatcaag taaaatcaaa tcaggttcta cctcagcaac tttttccaag gcttcttccc
    22801 catcatatgc agtataaacg tcatagcctt ctttggctaa attgaatttg acgatatccg
    22861 aaatcggctt ctcatcatcg acaactaaaa cttttttcat aaagagccac ctcttatata
    22921 ttattgtcat accttctcat tataccgtat ttctgctttt gtttcatctt tgcctataaa
    22981 acaaaagaga aaattaaaat gaaagtgcaa aatactttat tattttttgt tctaacttca
    23041 ataaaaaagg aaaacactga ttttcacctc agtgtttcct ttttttgatt tatccttcta
    23101 aaacttgtac accgacaccc aataatcccg gaccagtgtg gactactaat gccggagaaa
    23161 tctggccaaa gtagatttgg tttgcttgtg ggaactctct tttaagtctc gcttccattt
    23221 caattgcttc ttctttcgca tccccatggg caacagctaa gttgaagacc tttgcattac
    23281 cgattttttc tttgacgtaa gcaaccgttt tatccaaact ttttttacgt cccctagctt
    23341 ttcctactgt gtaataaatc ccttcttcat tacatgagat gattgggtta agctttaaag
    23401 cggttcctac aatagaagca actaaaccga tccgtccacc tttttgaaga tattccaatg
    23461 tcgcaacatt aaagaaaact tttgttcttt cgactaactc attcagatta ctgatcagtt
    23521 gatcccattc tactccttct tccagccatt ttgctgcttg gatcgcagaa aaacctgcac
    23581 caataccaat atttttcgta tctagtacat atgcttccaa ttcttcttgc tgttgtgcaa
    23641 ttaaacgaac cacattatat gtcccgctca atccactgga aattgttacc gcaagtacct
    23701 tttcataacc gtcttttttg atttgctcaa agatttctgt gatcgactca ccatccggca
    23761 aagatgtacc agggatttcc tctggcaaac gttgatagat ctcttcaggg gtaatgtcaa
    23821 ttttatctgt atatacctga tctttataaa taattttcaa tgggatcata tacatctcgt
    23881 atttttttat caattcttct ggaacatccg tacctgaatc tactaaaatt gcgattttat
    23941 tgtttttcat ttgttttctc ctcttttaaa tattcggttt ctagaacaat cgttttttcc
    24001 gcaagaagtt tcattgcaaa cgataaagtt gccgctctta cagcaatata ttctacaggg
    24061 gttgcctggt cgaatttttg cggatgagat ttaccttgtg ctaattggct gaccatccac
    24121 acagctgctt cttgttcatc gcaaaataga ttataggctt cacgaattcc tactgttttt
    24181 ccttggaaca atatcccttc tttgatttca ggaatagtca atacttgttt caacgtagtg
    24241 attgcaagta aaaatgctac gtgttctttg ttatatcttt ttttaactgg tgctggtatc
    24301 aaacctagtt taacgtaatt gtttaccata gaagaggtaa gcaatggatg cttttctcct
    24361 tgtatcacag gtgaaagata acgatcaact aacgttctga cttggtccat ataaagttct
    24421 aagtcaggga gttcttccca gtgaggcaga cggaatgttt ctagttcctt cccccactcc
    24481 attaattgct gacgcatttt ttccatcata accgctcctt ttttcatttc ataaacatga
    24541 tatcagactt atgataaaca ttcaatctag ttgctttaac taggtgattt gattagcggg
    24601 attccatgaa aacaaaaaac aagaaggcta agccttcttg tcttttatcg ttaagaacag
    24661 gagacccgcc ggggagacgg cgggaaagga gtaaaaaatg aaaaaagtgt tagggtttgt
    24721 tctcttgcaa gaactttgtt tgttggtatg cttatataat agcgagcaaa tgtgaataat
    24781 ctatgaatga tttcctataa ggaagtaaag atttttctga ttttctttaa atgtagtctt
    24841 gcattttaaa attcttagtg ctactattat taagtacttt ttgtacgggc cgttagctca
    24901 gttggtagag cagctgactc ttaatcagcg ggtcgcgggt tcgagcccct cacggcccat
    24961 tgggtgccaa acccacggaa caggtaaatg ttggtgcgaa agcatttcga aaacatttac
    25021 gctgttcttt ttttattttt caaaataact agggcttttt atttttaact caaagtccac
    25081 tgtagccctt tggactttga gttaaaaaag aagcataatt atgaacagta aagtttctaa
    25141 tataacaata attcgagaag ctttgcaaaa tcatgctgcc aatgaactct ctaaacatat
    25201 aggattaaat ctaagtacta taaaaggtta aaaagtgaag aatatttaat agaaaaacta
    25261 aatttacttg ctgctttccc cttttccatt aatacaacaa tcagagagtg tctttttttc
    25321 gaaaaaatta gaataaataa aagacacgta gtttttttat ctacatgtct tccatcgact
    25381 acaagttatc atttctcaaa acgtatttag cttttaattg attaatcgtt tcacgttgtc
    25441 tacacattgc ctaattaaga ttttcttatt agaagcatca atcccgcaat aatacctaaa
    25501 actccaggaa ttacccacaa taatgttaaa agaaaaaatg gaataataga taaaatgagt
    25561 aaactaatac catagaccac tgttttttga ttttttctta ctaaaaaacc tattatgaaa
    25621 atcattatcg aaaaaactag ggtaattcct ccaaataaaa caacattccc gcttagattg
    25681 actaacatat ctatatcctt actggttagt tctgtagtat ttaaagtagt tgaatcaata
    25741 gaagatagaa acataccacc catcaatgct aaaagactac ataaaatccc tgttattcca
    25801 ctaattaaaa tcaaaataaa ctctgttttt ctcttattca tttaattctc tccagattaa
    25861 gtgttttaga attagaaatt ttttgaccat agttaatagc ttctaatgaa cgaataacaa
    25921 ccactgacgt gttagaaaaa taatccccaa tatgttgttt cttgggcgtg aaagcagcca
    25981 ccaaataccc aatttgaaat acaaaatttt ttaatataaa cctacatata cactcacgaa
    26041 taaagatagt ttgccaactt agctcttttt cctcaaagct cactactctt atcccaaaaa
    26101 tcattttccc aattgtttgt cctgaattca gcttagtcaa aagtacaaaa taggttaaat
    26161 aaatagctaa tgacgctata ccataaaaag taataatgga tttgtcagat tccagtccca
    26221 tgattttgta aaagagacct atagtagcat gggtgatgat gctaatacaa attaaatcaa
    26281 taagaaaagc aaaaaatcgt aaccaaaatc ccgcaaatac ataattaggc aaagtttcaa
    26341 tgccactctt cttattcatt ttatagccct ccatagtaat acattggttt tggagtatca
    26401 gaaaatccaa gtttttcaaa aactgaaatt acacgatctg atgtagttgc ctttagccct
    26461 tgccattcag caatcttatt acctaaccaa gaagaagcaa agccagttgt gtcattttta
    26521 tattctataa ccgtcgcatc agttaatttc ttctcttttt ttagggaatc taatgcatct
    26581 tcagagtaac caatttgatc caccaaccca ttttctactg cttggttacc atcataaatt
    26641 cttccatccg caagttcttt cactgcatta gtatccatat ttcttccttt tgctacaaca
    26701 ttgacaaaac gatcataagc actcatcaca aattcctgta taactttatt ctcttcctct
    26761 gaaggtttgt gttgaggtga catcatatct tttaatgcac cacttttata cgtcgcatca
    26821 gttacgccaa gtttttctaa taagccagaa taatttaaac cagaaataat gacaccaatt
    26881 gatccagttg ttgtttcttc cgtagcaaat attttatctg aaccagcaga aatgtagtac
    26941 cctccacttg ccgctgtatt tttaaatgca gtatagatag gaatatctag ctttttaatt
    27001 tttgccattt ctttagcaat ctcagcactt tcatatattc ctcccccagg tgaatttact
    27061 tctaataaca ctcccttaac cgctttgtct tcttgaattt tctttagctg tgttaaaaaa
    27121 ttctgatggt tatattgttc cctactaaaa agattgcttt cacctgtatc tgcaataact
    27181 ccattgacgg atagtttgac aatcttttta tttgatgcgc cttcttctaa cgtttcttct
    27241 gtgatttcgt tagatccgta aaaaatctta tttattcctg ttagggttgt atttgatgct
    27301 tcctctttat cttttcctgg aattagcgcg acgataatgg atagtaacaa taatccgcac
    27361 gctactccca cagcaatcca tcttctttta ttcatataaa
//
LOCUS       DACDIE010000035        26869 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.035, whole genome shotgun sequence.
ACCESSION   DACDIE010000035 DACDIE010000000
VERSION     DACDIE010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26869
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.035"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            203..1066
                     /locus_tag="IV250_001634"
     CDS             203..1066
                     /locus_tag="IV250_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP8768921.1"
                     /translation="MLMSIRFVDFGYKISHSIIGLAIVMFSLLVAPYVQLIKWSVMGM
                     LIHFILLSSILMATASDPKMGNASLYGFSYLFIVYSLPKDSLNKNFFTQTSSLLFLFF
                     CWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQEKIWMLIYAFGISLLIVAGEYVPFQR
                     LMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGSLIGCVLFIGISQFIPFNWVGILGGL
                     ALGVCSTYRYKTVFNSFGALAITASLFGVPGAVTIRIFENILGVCLGIMYIGVTEILI
                     RKIREKHGLNH"
     gene            complement(1144..1437)
                     /locus_tag="IV250_001635"
     CDS             complement(1144..1437)
                     /locus_tag="IV250_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAP8768922.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            1605..1919
                     /locus_tag="IV250_001636"
     CDS             1605..1919
                     /locus_tag="IV250_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAP8768923.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            1940..3919
                     /locus_tag="IV250_001637"
     CDS             1940..3919
                     /locus_tag="IV250_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP8768924.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            3932..4258
                     /locus_tag="IV250_001638"
     CDS             3932..4258
                     /locus_tag="IV250_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAP8768925.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            4274..4792
                     /locus_tag="IV250_001639"
     CDS             4274..4792
                     /locus_tag="IV250_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8768926.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            4792..6144
                     /gene="celB"
                     /locus_tag="IV250_001640"
     CDS             4792..6144
                     /gene="celB"
                     /locus_tag="IV250_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP8768927.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(6228..7733)
                     /locus_tag="IV250_001641"
     CDS             complement(6228..7733)
                     /locus_tag="IV250_001641"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HAP8768928.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(7825..8415)
                     /locus_tag="IV250_001642"
     CDS             complement(7825..8415)
                     /locus_tag="IV250_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8768929.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            8594..10240
                     /locus_tag="IV250_001643"
     CDS             8594..10240
                     /locus_tag="IV250_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP8768930.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            10289..11008
                     /locus_tag="IV250_001644"
     CDS             10289..11008
                     /locus_tag="IV250_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP8768931.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            11487..12434
                     /locus_tag="IV250_001645"
     CDS             11487..12434
                     /locus_tag="IV250_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8768932.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            12446..13366
                     /locus_tag="IV250_001646"
     CDS             12446..13366
                     /locus_tag="IV250_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8768933.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            13407..14864
                     /locus_tag="IV250_001647"
     CDS             13407..14864
                     /locus_tag="IV250_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8768934.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            15209..15823
                     /locus_tag="IV250_001648"
     CDS             15209..15823
                     /locus_tag="IV250_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP8768935.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            15816..17540
                     /locus_tag="IV250_001649"
     CDS             15816..17540
                     /locus_tag="IV250_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP8768936.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            17537..18988
                     /locus_tag="IV250_001650"
     CDS             17537..18988
                     /locus_tag="IV250_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8768937.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(19461..21614)
                     /locus_tag="IV250_001651"
     CDS             complement(19461..21614)
                     /locus_tag="IV250_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP8768938.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(21616..22662)
                     /locus_tag="IV250_001652"
     CDS             complement(21616..22662)
                     /locus_tag="IV250_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8768939.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            22812..24101
                     /locus_tag="IV250_001653"
     CDS             22812..24101
                     /locus_tag="IV250_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAP8768940.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            24114..26816
                     /locus_tag="IV250_001654"
     CDS             24114..26816
                     /locus_tag="IV250_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP8768941.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
BASE COUNT         8700 a         4652 c         5433 g         8084 t
ORIGIN      
        1 tttttatcag tctctacaac tagatccgtt tattttaaaa caaaaaatac gagaagcagc
       61 ttctaaaaaa gaaaagtgca tgtatatttg ttcattgttt ttacgatcat tattcatcgt
      121 attgtttgcc atttgtttta tcattataat tacaacatta tttgaatcga agcataaacc
      181 atacgctgtg gttttattct gtatgttgat gagtattcgt tttgttgact ttggttataa
      241 gatttcccat tcgatcatag gtttagcaat cgtcatgttc tcactattag tagcaccgta
      301 tgtccagctg atcaaatggt cagttatggg catgttgatt cattttattt tactaagcag
      361 catattaatg gcaaccgcta gtgatcccaa aatggggaat gcttctttgt acggcttttc
      421 atatctcttt attgtttatt cgttacctaa agattcgttg aacaagaatt tctttacaca
      481 aacaagtagc ttattatttt tgtttttttg ttggttttca gttctccttt acagaaaaca
      541 tcgagaaaag aacaaagaca aaagcttgtt taaagagatt tttttgaaag gcatgtattc
      601 gcaggaaaaa atttggatgc tcatctatgc atttggcatt agtcttttga tcgttgcagg
      661 agagtatgtc ccttttcagc gattgatgtg ggcagggttt gcattttctt ctattgtatc
      721 tagctatgga ttgatgtcga ttggtttcaa agaacgagca gtagaccgaa tcattggctc
      781 attgattgga tgtgttttat ttattggtat cagccaattt attcctttta actgggtcgg
      841 tatattaggt ggtctagctt taggtgtttg ttctacctat agatataaaa cagtttttaa
      901 ttcttttggt gctttggcaa tcactgccag tttattcggg gttccaggag cagtaacgat
      961 ccgcattttt gagaatatcc ttggggtatg tttaggaatc atgtatattg gggtgactga
     1021 aatacttatc cgaaaaataa gagaaaaaca cggtttgaat cattagatga gatcgaataa
     1081 aaaataagga aaaagtgaag atacacatgg atgataaatt gctatcattc catgtgtatc
     1141 tttttacggt aaatcagtga aacgttcgac tttcattttt aatttatact ttttacggag
     1201 ttcgtttatt tgagccatca tttcggattt gtgatgcttt tcgattgctt cttcatcttt
     1261 ccaacggtcg atcagtaaaa gagattctgg atcttctagt ggaaagtaat agtcatattt
     1321 ctcgtttcct tcttctgcac gaatcgcttt gacaatgttt ctttcgacca tttcttctgc
     1381 aaattttctg gcttgtccat ttgtacttgt atacaaaata ttcactgtga tactcactaa
     1441 atcccctcct gaaaaaattc tatcactagt atatcacggt gccagaaatt attattcaag
     1501 atatgataca cattttttcc gcaatgctac ggaaatatat attttgaata gaaaacgctt
     1561 acaagatatg cttggtttat aaaaataaaa ggagtgattg atttatgaaa aaagcattga
     1621 ttatttgtgc ggcaggaatg tcatcctcga tgatggcctc aaagacaacg gatttcttta
     1681 agcaaaaaag agaagaggta ctcgtagatg cagtttcagc tacagaaggc gataatatga
     1741 ttaagaatag tgattttgat ttatttctga tcagcccgca aacgacgatg tttttggata
     1801 aattcaaaaa attaggagaa tctgttggaa agcctgttgt tagtatccct ttccaagcgt
     1861 atgtcccaat ccctacaggc atccagaagt tagcagaggt gatcgaagag aacatatgat
     1921 ggaataggag tggatgataa tgaaacagaa ggaaaaattg ctgttgagat atttgattga
     1981 tcacaagaag gagtatgtca ccagtcaacg tttagcttct gaattgttat tatctgatcg
     2041 gacaatccgt aattacttgc agcggatcaa agagttagta gaaaaaaacg gaggtaagat
     2101 cattgctaaa ccaggttatg gctaccaact gcatatttta caaagactga catttgactt
     2161 gtttttgagt cggcaagaaa tagtacctgg atatgctaga gaaccgcaag gattttatga
     2221 gtccgaagat cgacaaaagt atatattgaa ccaattgctt ttggaagatg ccattctttt
     2281 tatggatgat ttggcagaag agctttatat cagtcgttcc agtttgacaa aggacatgca
     2341 agaaatcaag gagcgattga ttccatattc actaaaaatc gtttctaagc atggtcaagg
     2401 gacatggatc gaaggggaag aaagaaatcg acggcatttt atcatggata ctttttttgg
     2461 aaaccattat ggaaattcat tgaaagaata tttagggcaa agccgttttt tcccagacat
     2521 cagttttgaa gagctagtca ttatcattct cgatgaaaca agagaagcta agttaaaagt
     2581 ttcggatttt atcatacaga atctgacttt acacttggct ttggggatca aacggctccg
     2641 agaagggttt gaaatcaagg aattaggcat cgaacaagaa gtttctaagc gagtagagta
     2701 tcaagtggcg cagcggatcg ttcaacgtat tgaggtaatc gcaggtgtat cgtttcccga
     2761 agaagaaatt gcttatttga cattacatct catggctaag tcgaatcaca aagaaaatca
     2821 aaatgatcag gaactatcaa aggaattagc gaaagttctg aaaagattgt ctcaggaatt
     2881 tcatttttca ttagtagagg atctccagct tttcaatgga ctactcgatc atttgtcacc
     2941 gatgatgatt cgattgagcc gaggtattcg cattgagaac ccattgactg aggagataaa
     3001 gaaagaaaat cccaaagtct ttgacgcagt aaaaaggcat ttcagcaaca tgccggcttt
     3061 gaaaaactat accatcaatg aagatgaatg ggcctatttg gctttgcatc tcatggcggc
     3121 attggaaaaa gaaagagctg cgcataaact acatgcattg attatctgtg caacgggtta
     3181 cggcagtgcc caactattaa agaatcgcgt agtcagtgaa ttcggtaaaa atattacagt
     3241 ggtaagtgta aaaggctatt atgagatcaa tgaaacaact ttaaaaggaa tcgacctgat
     3301 tatttcttct attgatcttt cgacaatgtt tttcaaaatt ccagttttac atgtcagtat
     3361 ttttttgaat gaagatgatg ttcgtaaaat acgaaaggta gttggagaat ctatacccta
     3421 tcattcctca gatagaccga taccagcatt ttctctacag caaaaaaagc agatttacac
     3481 cgaacagttg tctgaaaaat ttttcaagac ttatacgtac gaaccgaaaa aggaagaaat
     3541 tcttcatgat ttgctgcaaa agctgagcat aaatgaagaa gaaggatata taagagaaat
     3601 gcgaaagcag atccagcaac gtgaaaatat gggacaaatc atattcagtg atacagtggc
     3661 agtcccacat cccgctgttc cagttggaat cgaaacaaag gtagccgttg cattgattcc
     3721 ttctgggatg cagtgggatc aatatcaaga aattcatttt gtttttttag tttcgccttc
     3781 ttatgtcgaa aatgaaggaa ttacggtcgt gactaaagca atcgttaggt tagttgacca
     3841 gctaaaagta cagcaggaaa tcttagctga acctattttt gagaatttca gcagtaaatt
     3901 tatcaaactg atcgaataag gaggaaaaca gatggctgaa aaaataaaca gtgaaacact
     3961 acaagtgact tcttttgata tcattttcca tagcggaaat gcacggacac tgatacatga
     4021 agcatttcgc tcgatgcgta aagagatgtt tgaagaagta gaagagaagt tgcgacaagc
     4081 aaatgaagag attcttcatg cgcatcagtc acaaactcaa cttctgaaag aatatgccag
     4141 cgggacaaaa atagaaatgg aaattatcat ggtccatgca caagatcatc tgatgacgac
     4201 gatgacatta ttggaagttg cacaggaaat gaattatttg tatcaaaaaa ataaatagaa
     4261 aggaaggatc attgtgcagg atacagtaaa aaggaaaatc gaattagaaa aaaagcaact
     4321 tagtttgaaa aacatgtatt tcaatcgtta tctaatcgta cgttacctga ctgccttttt
     4381 ctttttcatg aacatgcaat ggatgattct gcttctatca gctaaaagtt taggaagtag
     4441 tttaccgatg attctgattt tagctatttt gcctgcagta ggagaacaag taaagcttta
     4501 tcgaaaacac caaacgaacg ttccatggac aaagtggtat tttttgttcc agagtgtctt
     4561 taatttatta gtgattccag tgttgtttac ctcaggattt acattcttgt atccttttat
     4621 ggcaaataac aatggaggac agttatttgt tttcattctt gttgtaagcg gtatttttgc
     4681 cagtgcattt atacagtatc gtttaaagaa aatttcactc aaccaagacc aacagtatat
     4741 ccgtattaaa caatatgaaa aagcgttata tttaggaaag gagaataact aatgtttgat
     4801 tttttgcaga aatacctgat gggacctatg gggaaaattg cgcaacttaa aatcgttcgg
     4861 gctgtcatgg cagcggggat ggcttctatt cctttcacga ttgtaggctc gatgtttttg
     4921 gttttaaata ttttgccatt accgttccca tttttagaag gattttttaa tgcaactttt
     4981 ttcaaagtta gtgacttgta catgatcgtc aatacaatga cgatggggat tttatctatt
     5041 tactttgcca ttgtgtttgc ttatgaatta acgtcaattg aacgagatga acaaaaatta
     5101 aatgttaatc cgctcacggg ggcattacta tctgtttttg cattctttat gtgtatccct
     5161 gaattgatca tgactgatgg taaaatcagc ttgattgctt ctatcaatga gacagaaact
     5221 gtagtaaatg gtattaggat gggctctttt gtcgaacgtt taggaacttc aggaatcttt
     5281 acagctatta ttatgtcgat tattgcggtc gaattgtatt gtatgtgtgt caagagaaat
     5341 tgggtcataa aaatgccaga tgttgttccg ccaggagttt ctcgttcttt cactgctctg
     5401 atcccaacat ttgtcattgc gtttgtggtg atgatcatca atggattatt agttgcacta
     5461 ggaacggata tcttcaaaat gattgctatc ccatttgggt ttgttacgca gttgacgaat
     5521 acatggcttg gtgtcatggt gatttatttc ttgatccatg cattgtggat cgtagggatc
     5581 catggagcga atatcatcac ttctttcttg accccgatcg tattagctaa tatggcagct
     5641 aacgcacaag gcgcaaatta tccgttggca ggagaattca acaattctta tgtaaccgtt
     5701 ggtggatcag gtgcgacttt aggattgatc atctttattg cattcctcgc gaaatctgat
     5761 cagctgaaag tgttaggaaa agcctcactt gtaccaggat tattcaatat caatgaacca
     5821 atcattttcg ggatgccaat cgtctacaat ccctatttag cggttccgtt tttcttagct
     5881 ccgatggctt ctgctacact cgcttatttt gccattcggc tggaaatcgt caaaccgatg
     5941 cttgcacaga tgccgtggcc ttcaccagtc gggatcggag catttgttgg tagtggtggt
     6001 gattggaaag ctgctgtgtt agcagtcatt tgtgcaatca tcgcattcat tatctggttc
     6061 ccatttatca aattctatga taataaatta ttaaatgaag aaagagaaaa ggcagcagaa
     6121 ttagctagtg aaaagttaaa atgatttcta agaaaaaagg aactgtgaca aaaaatcaca
     6181 gtcccttttt ttcttagaaa tgggaaacca ctagtatata aataccatta ttcttcaatc
     6241 aaccgcttca cgattgcata atccccttca taaggttcat ctacaccatt gattttttga
     6301 tagggatcca cgccttttcc cgcaataacg actgaatctt gcggagcgct ttctgctaaa
     6361 gcgcgtcgaa tcgcttcagg acgatccatt tcataatgaa cgatcaaatc tggattagta
     6421 attgcttcat tgatttcttc cgcgattttt ttcggatctt caaatgctgg gtcatctgcc
     6481 gtcaaaaagg cgatgtcagc tgtttctgat agcacttttc caaaatcatg tcgacgggag
     6541 attgctttgt ttcctgggct gcctaataca acaattacac ggccatctgg atgttcattt
     6601 ttggcaaatt tcagcaatgt tttcaagctt agataattat gtgcatagtc cacatagaca
     6661 tgcgctccat tggattggat caattgatcc attcggcctg gtacacgagc ttcttttaat
     6721 ccgtcttgtg catcttgact ggaggcacct gttagtgctg cggcaatgat cgcactggct
     6781 gcattttctt ggttgaatcc tccagccagc aaaatatcat aagcagtatc gcagatagct
     6841 aagcgatctt catgagaaga taaagtaaaa ccgtgagtac ctttatctga acggatcact
     6901 tgataattgc tttcttccga acgaccataa gtgatcgttg gaatctggaa tagatcggct
     6961 gtttccctaa gtaaatcaaa atagtcactt tcatgattta aaacaactgt ttttgaattc
     7021 aagatcaatt gtcgcttgca atagaaatag tcatcaaatg tcggatgttc gatcggactg
     7081 atatggtcag gcgagatatt caagaagatt ccgacatcta aagtcaagcc gtatacgcgt
     7141 tgtgttttgt aagcttgtga agaaacttcc atcactagat gagtcatacc attttcaact
     7201 gcttctgaca tcatacgata taaatctaat gactcaggcg ttgtcaaatg ggatttgaaa
     7261 tatgttttgc catctaacgt tgtattcata gtagaaagca atgctgtttt tttatttgtt
     7321 gtgtgatcca agattgcttt tgtaaagtac gcagcagttg ttttaccttt tgttccagtg
     7381 aatccgatca ctttcaattt attttgcgga tagtcgtaaa aagccatagc aagaagcgcc
     7441 attgctttac ggatatccgt tacgatgatt cctactgctg aattttcgta tggttgttct
     7501 gatacataat attgaatacc ctgttcgatt gctgaatcca aatattcttc tttgaaattc
     7561 attcctttac agaaaaacaa agtagaagca tcagcttttc ttgaatcata acttaactga
     7621 agaaattcag ctttttctgg tacatcaaga tgccagcctc ttgttgaaac aaattctttt
     7681 aatagatttt cttttaatag taaatgacgg atgtcttcta aagagaagct cattgattga
     7741 tccctcgctt tattcaattt cttggtttat tatagcacac tctttttaat atatcgtttg
     7801 tttccgctat gacaaaatcc agctttattc aaattttctt gacacagggg acaagcggat
     7861 gagtattttc ataaacaatg aactgactgt aatagatagg agaacgaata gtgtctccaa
     7921 caatccaaaa acataataat tcaaaaagac gataagacta ttggtaagta aaagtccttg
     7981 cggcattgga agccgaaaaa atttgtggaa aataatcggc ggtatcgtta taccgccatt
     8041 tgaggccccg actgtgtaca atgtgtaaac cccaagactg aacaaaagac ttcctgaaat
     8101 cagtgaaaag aaatgtgagg agatcatttc cacttttgga acaatctcta aaacaacagg
     8161 aaaaagcaga cttcccgcta cagtcttaaa aaatactgat tttcctaata ggacagcggc
     8221 tagaagcagc atcataagat tgatgctcca aacagtaact gagcgttgga tagaaaaggc
     8281 cgtttcaact aaatgtccga tggcgcccac tccggcggaa gctatatcgt gagggagcaa
     8341 gaaccaattc aatccaactg tgataaagaa aagacctaaa aatataacgg gtagtttttt
     8401 tatttcttgt gtcattgata tctcctataa atgttagcca taaaaggctg tatatttctt
     8461 tactttacca aaaaaacgaa accgaattca aaaaaacaat tgttcttata aaagttatat
     8521 aaaatcgatt gccagtttct gttttaccaa ttaaaatgat agtaatgttt tgtagaaagt
     8581 tggaatctga ccgatggata atcaaagaaa accagcatta gatcaattaa caccgcaaga
     8641 aaaaatggca cgaggctctg cttggatgac cgccagtaat atcatttcaa gattgctagg
     8701 cgctgtctat atcattccct ggtatgcttg gatgggcgaa aatgctaagg ctgccaatgg
     8761 attattcaat atgggataca atatatatgc attgtttcta ctgatctcta cagcaggaat
     8821 acctgctgcg attgcaaaac aaactgcacg ctataattca ttgaatgaat atggcacttc
     8881 acgacgatta tttatccgtg cattacagat gatgggcggg ttaggcctgt tgtttgcttt
     8941 gttcatgtac atcgcctctc cttggctagc tcatgcttct ggtggcggag aagaactgat
     9001 tccaacgatg cgttctttga gtatcgctgt attggttttt ccttgtatga gtgtgatacg
     9061 agggtatttg caaggcaatc aagaaatgat gccttatgcg ctgtcgcaga ttgtagaaca
     9121 ggtggcacgt attttttata tgctattggc tacattcatc atcatgaaag tcatggatgg
     9181 tgactatgtg acggcggtca cgcaatcaac tttcgcggca tttatcggta tgctagcaag
     9241 tattcttgtc cttttgtatt ttttgaagaa acatcaagcg tatacatcgg cgtttattca
     9301 ctacagcgaa aacaaaataa atattgcaac gaaggaattg ttgttagata ccgttaaaga
     9361 agcgattccg tttattattg tgggttcagg ggtaacgatc ttcaaattag tcgatcagtt
     9421 cacttttatg aagatcatgt ctgcatccac cgagtattcc aatgcacagt tactggatct
     9481 tttttcgatt tttagtgcca atccagataa gttgacgatg gtagttattg cattggcaac
     9541 atcgatcgcg tctactgggt taccgttgat tacagaagct gttacgttga aagatcgagt
     9601 aggtctggca aaattgacaa gcggaaattt acagttgttt tcatttttta tgtttccggc
     9661 gacatttggt gttatgttac ttgcctatcc gctaaataca ttattttata caccggattc
     9721 tctaggaagt aatgtactga tccaagcaag ttttgtagga ctcttcctag gactatatat
     9781 gctagtttcc aacatgctgc aaggaatgtt tgaaaacaag gcggcaatta attatttggt
     9841 tattgggttt ctagtgaaac tccttcttca atacccagcc attcgtttat ttgaagtata
     9901 cggcccttta ttggcgacaa tgatcggatt tgctgtgtct tgtaccttga tcttaaagag
     9961 aatccatgaa gtagcccgct ttaaaccaag ttttgtctgg cgcaggacat tgctgatctt
    10021 tattcttacc ttgattatgc ttttggcttc gtggatcaca aagatgattt ttggtgcttt
    10081 tttaaacgaa gatagcaaat tccagtctct attgttgatt cttgtagtag caggtatagg
    10141 ggctttagtg tatggttatt tagctttgaa aatccgcttg gcagaaagac tactaggacc
    10201 aggaatagcg cgtttacgac ataaattaaa aataaaataa gtgaatcaga agagctagat
    10261 tgatgctctt ttgaaatggg ggaaacgcat gcgtttagat aagtttttag cagatatggg
    10321 acttggcagc cgcaaagaag tcaaaggatt aatcaaaaaa ggaacgatcg aagtcaatgg
    10381 acaagtggtg aaaagcgata aagcgcaagt aaatgaattc gaagatgcgg ttacttattt
    10441 aggagaaatc attacgtatc aaaaagattt ttactacatt ttgcataaac ctgcaggagt
    10501 catttctgct actcaggata atcatgatca aaccgtaatc gatttattgg aagaccagga
    10561 ttatcgggaa gatctttttc cagtcggccg attagataaa gacacagagg gcctcttgat
    10621 tttaacgaat gacggggtgt ttgcccatca gttactatca cctaaaaaac atgtggagaa
    10681 agaatatcta gctgaggtta gcggtataat gacagaagaa gatatccgtt tatttgcaga
    10741 aggactgatt attgatggcg atgagcagac attgccggca aaactccaaa ttgatcaagt
    10801 agatcaagag acagaaactt caattatccg tctgattttg catgaaggaa agtttcacca
    10861 agttaagcga atggtcaaag cagtaggaaa agaagtctta tatttaaaaa gaatccgtat
    10921 gggaaaattt tggctgccta aagatttgaa taaagggcaa tatcgaccca tgaccaaaga
    10981 agagctagaa caagtgagag aaaaataatt gaatggatag ctaaaagaat aagcagagta
    11041 gttgtagcgc catctgaaaa gcagacttgt ccgtctgact ttcggatggc ttttttgtga
    11101 aatttcggct tatttccgta gaagctactt ctggaacacc gtttgttcgg aataagggac
    11161 tgtgacaaga cttttgttac agtccccatt gttccttgtg actcagagtt agatagtaag
    11221 gacacgatct ccctaatact caggccaaaa cgctgaattc acaacctctg ataaacggtg
    11281 ttcaatcagc tgaccctcgg tattagctca aaaaacggca aaatatgagg aactattttc
    11341 ccgttttttt ctctaatact caggccaaaa cgctgatttc acaacctctc ctatactttg
    11401 tttcattttc cctatacttt gtttatttat tctcattatt gaaagcgcta taattacgtt
    11461 gcgaatgaat cgaaaggggt ttttagatga aaaaacgaaa ggggttcaat cattttttta
    11521 tgaatctatg gcgttataaa gcacttgtcc tgatggctgt accgggtatg ctttggatga
    11581 tttttttctt ttacatacct gtaatggcga atgtcgtagc attcaaaaat tttcatattt
    11641 cagcagatgg gttcttggct agcttgaggg aaagtccttg ggtgggttgg gaaaatttca
    11701 aatttttatt ttcttcggat caagcatttc tcatcactaa aaatacaatt ttatataatg
    11761 tagcatttat ccttttgaat ctgctgatct cagtcgtatt tgcaatcatt atgagcgagt
    11821 tgagaaataa acgactcgtt aaagtttatc aaacgatgtc cttactacct tattttctat
    11881 cttgggtcat tatcagttac tttgtttacg cttttttaag tcctgacaaa ggaatattca
    11941 atcagtggat cactggacat ggaggagaag ctgtcaattg gtataatgaa ccgaaatatt
    12001 ggccgtttat cttggtgttc attggtacat ggaaagggat tggttataac agcattattt
    12061 actttgcatc tgttatggga atcgatccga cttattatga agctgctatg gttgatggag
    12121 ctagcaaatg gcagcagatc aaacatgtga cgataccaca acttattcca ctaatgacga
    12181 ttttaacgat tttagccgta ggaaacattt tccgagctga ttttggcctg ttttataaca
    12241 ttcctaggaa ttccggtgca ttatatgatg tgactcaagt tttggatacg tatatctaca
    12301 atggcctgac atccacagga gattttggta tgacggctgc tgcaggactt taccaatcag
    12361 ttgtcggctt tgtcttgttg atgatcacaa atacgatcgc ccgacgtttt gatagcgatt
    12421 ctgcattatt ctaaggagga aaaaaatgaa aaaaaagaaa gtggagagag tccaagtccg
    12481 ctctttcaat aagacggcta attttttctc taatataatg attgcactct ttgctatttc
    12541 ctgtattttg ccatttatat ttgttatcgc catctctttt accgatgagt ccatattgtc
    12601 gactcacggg tacagttttt ggccagaaaa gttcagtata tttggttata cgtacttgtt
    12661 cgaccagatg caggataaaa ttttccaagc gcttttcgtc actgtacttg ttactgtttt
    12721 aggtacactg atcaatagca cggctacttc tttatatgct tatgcgattt cacggtcaaa
    12781 ctttccattc cgtcgttttt ttaccgtttt ttgtttgatt acgatgttgt tctcaccagg
    12841 gatggtggcc aattatcttg ttatgacaaa tcttcttcag ttaaaagata cgatttgggc
    12901 attgatcttg ccaatggctg tcagtccctt caatattatt gtgatgcgca cattctttaa
    12961 acgttcggtc tctgattcga ttattgaatc ggcacggatc gatggagcta gcgaactgcg
    13021 aatatttgtt cagatcgttt taccgttagc tgttccaggt attgcgacaa tcagtttatt
    13081 tgccgcattg ggttactgga atgactggtt caatgcttta ctatacatcc aagatgataa
    13141 gctagttcca ctacagtatc ttttgatgaa aatccaaagt aatattcaat atctgacaca
    13201 aaattcagga gcaggaagcc aattgacagg cggattagcc tcagttccag gagaatctgc
    13261 cagaatggcg atcgtagtca tctcgacgtt gcctattgcg atcagctatc catttttcca
    13321 gaagcatttt gtcaaaggtc taacaatcgg cggagtaaaa gaataaagaa acaaaaatat
    13381 aaatcataaa attaggagga agaaaaatga agttatggaa gaaactagcc tttacaggtg
    13441 tttcagcgtt gttgttggga acattagcag catgcggagg ttctggttct aaagaccagg
    13501 cagaagctag caattcggac actttgcaga tgtatcaaat cggagataaa ccagataatt
    13561 tcgatcaatt gatggaagta gcaaacaaac gaatcgaaaa agagatcggt gtaaaagtca
    13621 atatcaatta cattggctgg ggcgattacg aaaagaaaat gaacgtcatc atttcttccg
    13681 gggaaaatta tgatatcgcc tttgcgaaca actatgtttc caatgcccaa aaaggagcct
    13741 ttgctgattt gactgaatta gcgcctaagt acgctgaaaa agcttataat gatttagatg
    13801 aagcatatat taaaggaaat ctggtcaatg gaaaattata tgctttccca gtcaatggaa
    13861 atgtctttgc tcaacaagta cttactttta acaaagcttt actagataaa tacgatcttt
    13921 caattgatgg aatccaatct tacgctgatg cagaaaaagt cttgaaggaa ttccatgaaa
    13981 aagaaccaaa cacggcagct tttgccattg gacaaggctt caaagtccaa gggaactttg
    14041 attatccttt agggaatacg ttaccatttg ctattgatct ggatggagat gattcgaaaa
    14101 tcatcaacca atacgataat gaaacgtata tcggactgtt acgtacaatg catagctggt
    14161 acaaacaagg actcattcca agtgacgctg caacgagcaa taaggattat ccactagaag
    14221 gaaatacttg gctaatgcgt ggagaaacac aaggaccgta tgattatggc gatacaattt
    14281 tgacaaatgc agcaaaacaa gaacttgtct caaaagcaat tactgttcca ctaaaatcta
    14341 cagctcaagc acaaatggca aattttgtcg tatcaaatac ttcaaaaaat aaagaaaaat
    14401 cagtagaact tctagggctg cttaacagtg atccagaatt attgaatggg cttgtttggg
    14461 ggatcgaggg agaagcatgg gaaaaagtcg atggttcaga tgataaaatc aaattgctag
    14521 atggttatca gccaaataca catatgtctg cttggaatac aggaaacaac aagattctat
    14581 atacacaaga gtctattacg gatgacatga ttgccaaacg tgatcaatcg attgctgatg
    14641 cagaaacttc accgatttta ggattcagct tcaacactga cagtgtcaaa acagaattaa
    14701 gtaatatttc taatgtcatg aatcaatatt tagatggatt gaatacaggg actgttgatc
    14761 cagatgaaac attaccgaaa ttgaaagacg ctttggacaa ggctggctat gataaggtat
    14821 tgaaagaaat gcaaaaacaa tatgatgagt tccgacaaga ataataatga ataaagacaa
    14881 aggggttgtg acaaaagacg tgtcacaacc tcttgttccg aataaacggt gttcaaaagc
    14941 cagtttctcg gtattaagct aaatatatgt aaaaatgtgg aaagccatat tcacaaaatt
    15001 tttcttacta ctcgaaacta agcggctatt tcacaaactc ttggtaaacg gtgttcaatc
    15061 agctaaccct cggtattcgc tcacaaaacg acaaaatatg aggaactatt tttccgtttg
    15121 tttctctaat aatcaggtca aggtgctgat tgcccaacct ttttttgtat aatgagaaag
    15181 tataaactta atgtttggag gagaagatat gttagggaag gttttggaag agttatttat
    15241 ccggatctgg gtcattataa aactaacctt gtatttctgg atatatacat ttgccggcgg
    15301 gatcatcttt ggattaggtg cggcatggaa aacagtcaat gaactatttt atttgtacgg
    15361 attcgagtat aaagaaatca caataaaaag aggctggaat atatataaaa gaaatttttt
    15421 aagaggcaat ctgctattta gtttattttt atctggtact gctttattga gttacaattt
    15481 atatttatct gttcaaatca aagggctcat ttttttagct attgatttta ttttactttt
    15541 tgccttgctc tgcctatttg ctacttatca atacagtatg attttagata gtgaatatac
    15601 gatctctgtt cctaatctgc taaagctaag ttttatttcg gttttttcca gcttttcgtc
    15661 ttttctaaaa ataattattg gtagcggtat aattttaggc gtgacttggc aattcaaagg
    15721 attgatcctg tttggtgtca ttggtctttt gacggtttgg aatggtacga tgacaacaca
    15781 ttggcgtgaa gaattagata aacagttgga aagttatgaa taagtttcgg attctatttt
    15841 caaaaggaac cttactaaat cgtctgttgc ggaaatacgc ggttatcatg atcggtgtaa
    15901 cgatgactgc taccgttatt tttagtatcc atacttggga acaaaatcaa aaacaggcag
    15961 aaaacatgac aagtgatgcc gttcaaagca caagcagaat gctgaatgat aaaacaacct
    16021 tgagtaggat catcaaaaac cagcttgtag ggaattccga aaagatcgaa aatgtgacga
    16081 cctacttaac aaaacccata gatcagtatc tgatgtatgt atacgaacag caaaacagta
    16141 ccgatgaatt agtttctttc ccaaatcaaa tcaaagatct ctacgcaaat tatgaagaac
    16201 taagtgcgat atatatcgtg ttgaatcaac taccggagta ttatgaatct tctagagaaa
    16261 acaaaggtgg ggaaataaaa aaggggacac caaaattact agacgccttt tatatcaagt
    16321 tgcctatcac acaaggagga gctgagtcag gaagtatttt tattggtttt caaaagaaag
    16381 aactagatat gattttagaa aatctaacgt cattcaatgg actctcttta tacatgattt
    16441 ctggaacgac aaatcgacta tacacattcc atgataataa aatagctaaa gaaacgtttg
    16501 aaagacaaga aatgatcatc acagagagtc tgaagaaaaa ttcacaattg ccgattgacg
    16561 atctggaaaa aaacaatttt gttcaacacc aagaactgtc tgatgattat cgtatattag
    16621 ctgtactaga taaaaaaagt gtaaaaatcg aaacgggaaa aaatcttgcc ccgctgatca
    16681 ttggtatcat tattttagac aatatactgc ttatctttct ttataaaaca ttcaaacgat
    16741 attcacagca agtatctatc gtcatgcagg ccatggatca aacagcagca gggaatttag
    16801 agacgcggat cgacataaca aacacggaat atgaactgaa ggaactttca atcggtatca
    16861 acgaaatgtt ggatagcatc aatcagtttg tagaagacat ctacaaatta gaaatcaaac
    16921 agcaagacgc acatatgcgg gcgcttcaag cacagatcaa tccacacttt ttatataata
    16981 cattagaata tatccgaatg tatgcaatca gcgaaggaag tgaagaatta gcagatgttg
    17041 tgtatgcgtt ttccgctttg cttcgaaaca atacgaatca agagaaaacg atcactttaa
    17101 aagaagaact ggacttttgc gaaaaatatg tctatttgta tcaaatgcgt tatcctaatc
    17161 gagtagccta tcattttatg atcgatcctg atttggaaaa gatcgaagta cctaaatttg
    17221 tgatccagcc attagttgaa aattatttta aacacggtat tgattttact cgattcgata
    17281 atgcgctaag tgtcaaagtc ttgcaggaag gcaaacgggt aaggatcatc atcaaagata
    17341 atggaaaagg gatgactgaa aaacggctaa aacagattga agaaaaactg tctcatccta
    17401 aagtggaact tcacggttct atcggtctgc aaaacgtgaa tgaacgttta cgtgccagct
    17461 tcggcagcag ttattatatg tcgcttgaga ataatgagac aggtggactg accgtttcta
    17521 ttacattcaa ggagggatga gcgtgtatca agtgttattg gtagatgatg aatacatgat
    17581 tttgaatggg ttaaaaaaga taattgattg gcaaagttta ggctttcaaa tagtagcaac
    17641 agcagagaat gccaaagaag gactagctgt acttgaacag cgacagattg atttagtagt
    17701 aacggatgtg acaatgccag aaataaatgg actggaattt attgaagcgg ctcaaaaaga
    17761 acgtcataat tttgaattta tgattttatc aggataccaa gaattcgatt atttgaaagg
    17821 aggtatgcaa ttaggcgccg tgaattattt gatgaaaccc gtgaataagt tcgaattggt
    17881 tgaaagcctc aagaaaatca aaacacggtt agatcagcaa aatgaacaaa aaaatcaaca
    17941 agaaatctat caagaactgt tattcagtca atggttgaat gaagaattgg atgaaataag
    18001 tgaagaagaa ataataggac aaataagtga gaagcagcat aggatagtat tgattcaact
    18061 ggcaagaact catgatactc ttatcaatag atggttgcaa gaacggcaac aaatctttta
    18121 ctatcaacga aattacggag accgtgtatt gatcacgttg ttgttggagg cagatgaagt
    18181 tgatggcttc tgttgttttg ttcaagaaaa ctttcctgat caggaatggc tgatcagtat
    18241 cggggaagag acagattctt tggacaaaat ccctgaaaca ttccaacaag cgaaagacaa
    18301 tctacagttg catcaatttt atggagataa agacgagcat gttttttacg cagtacaagc
    18361 agaaacaaaa gatcaaacca tcgatttttc aagtttcaga cgcgtgctgc aaaataaaca
    18421 attggaagtc gctcaaaaaa tgatcactga tttttttgaa cagtttcagt tagcagccat
    18481 gatgccggaa gatatacgat attccagttt tttactgttt atggagattc aacgggaatt
    18541 gattgatctg gaagatgagg aatatttaca aggaatcgaa aaaatccatc aggcaaaaac
    18601 ggtgcaagaa cttcagcagc tacttctgtc attcatcaaa aagcatcaga gacagaaaaa
    18661 gtacagtgac aatgtggaaa aagtaatcgt aatcctgcac cagcattatc aagaaccact
    18721 tacactcaaa gaagtgtcag aaaacctgca tttaaatgtc atgtatctag gacagctgtt
    18781 caaaaaagaa acgaaaaaaa gtttttcagc ttatttgaac catttacgta tggaaaaagc
    18841 gaaacaactg cttttgcaca gtaatcaaaa catcaatgag atagcaagtg agattggcta
    18901 caacaacacc acgtatttct ctaaattatt caagaaaatc gtcggcagat cccctaaaga
    18961 gtaccgagaa aacagtggag aatcataaaa gaaaaagcca atgtaatgaa ataacttata
    19021 aaaaattgtc tagaaatcta attttgggct gttcttatta cttgaggcaa ggcagctttt
    19081 tcatgacctc tcgataaacg gagttcaaaa gccagtttct cggtattaag ccaaatttat
    19141 gcaaaaatgt ggaaagccat tttcacaaaa tttttcttaa tactcgaaac taaacagctt
    19201 tttcacgacc tctccactaa acggtgttca atcagctgac cctcggtatt agctcaaaaa
    19261 atggcaaaat atgaggaact attttgccat ttgtctctct aatactcagg tcagaacgct
    19321 gatttcacaa cctctcaata aacggtgttc aaaagcttgc atctgcggta ataagaccaa
    19381 ccaaacgaaa aatatgagga tctatttttg cttggctgtt cttattactt gatgcaggac
    19441 agctttttca caacctcttg ttaattatag tttgataagg aaaaaggcaa atcttttgct
    19501 tgatatttct tagggtgagg gatgatccct aattggtaag agatcgtgcc gcctttcagc
    19561 aaatcttgat gggtaaaata taatttcgta tattctttgt ccatccgttt gattttatgg
    19621 ataaattgtt gctgcggccg attaggcgtt gcttcaatcg tcaattcctt tccatttgac
    19681 aaaagcaaaa cagccttgtc aattaatggc ataccgataa catattcacc ggttccaggc
    19741 gtgacaggat aaaatcccag actattgaag atataccaag cggccatact tccattgtcc
    19801 tcatctccag ggtaacccat tggagaatcg tcgaaggtct gagtcatcaa ctgtttaagc
    19861 agtggctgtg ccatttcagg ttgtcctata taactgaaca agtaaggata gtgaaaactt
    19921 ggctggtttg aaatagctaa ttgtccaaat tctatcgcgg ccatctcact catttcgtgg
    19981 atctcaaaac catatcctcc tgggtcaaac gttgggggct gattgcacag ctcaatgagt
    20041 ttttctttat atctctcact gccaccatat tttccgatca gtcctgaaaa atcgtgataa
    20101 acagcaaagc tgctttgcca agcacttcct tctgcataat cttgtcccca acgattagct
    20161 gaaaatggtt ctctgaactt gccatcttta tcttttgaac gcataaatcc tgactcatag
    20221 tcaaagatat tttgatagtt caatgattgc tgctgatatt tcttcgtcat ttcctcttct
    20281 tctagtacct tggcaacttg gctaatacaa aaatcgctat aagcatagtc taatgtgtga
    20341 ttgactgatt cgtggtagtg attaggaaca tagccatatg tcaagtaatc ttctgttcct
    20401 tggcgtccat aatttggatg tttgctttga accgtagcac ctttttccat tgccgttaga
    20461 aattcaggca tcaagtctcg tcggatatct tttacagcag catctgcaat gaccgcatcg
    20521 atcaatgtcc caggcataag tccacgttca tcaggagaca gccattttgg caaatagcca
    20581 ctttctctat agctgttcaa aaaaccttcc agcatttctt cgtatttttc tacagcaatc
    20641 aaagaataaa gaggataaac cgttttatac gtatcccaaa acccattatt tgtatagaga
    20701 acaccaggtt ttatagatcg actagtcgta tcatagtgga ttttttgccc actaggatca
    20761 atttcataaa atgtttgtgg aaataaaaac acccgatata aattatgata aaacgtccgc
    20821 aattgttgtt gatcatggtg tgtgagctgt atacgatcaa aataggactg ccattgttct
    20881 tgactgtgag caaggtaagt ctctttgctt tggttttgtt ctagttccag gttataagct
    20941 gcttgttccg agctgataaa agatgtccca aatcggatag tttgtttttt tgtctgttca
    21001 aaatacaacg ggatcgagtc gctatttttg ttggaaacag ctaactcatc aattgttaaa
    21061 ggatattcaa atctcaatat aaaataaaaa gaaaaatcag gatcttctga accagcaaaa
    21121 tgtgtgattt ttccttccac tgtgtaagga tctataacaa agagctgatg gcgtcccgga
    21181 aaggataaaa gtaaaccgct gtcatttttg ctgtaatcaa tagataagat tccgccatac
    21241 atgctaggta tcaatgtaga acagatgcca taacgtaatt gacagatcga cagatgagtc
    21301 gggcaaaata tactttcttc tgggcggtaa gaactttgag catggaaaag ggtgttctcc
    21361 tgtagttttc cattgatcgg cgtaaacagc atatgactga aatcacccat ccaaggactt
    21421 ggttgatggg taagacggta gccttgaaaa acgcggtcat caggatgaaa ccaccagctg
    21481 ccgttttgat ccgtagtttg cggtgcaaaa taattcatcc caaaaggaac gccagtataa
    21541 ggctggcagt tcccattaga gaaatttggc tgattcgctg ttgcatgtct agtatctatt
    21601 tgaccgatat tcattttatt tctcctttgt tgattgtctt ttgatgaatg aaactggaaa
    21661 tttactatcg gatggtcgat cttttgtctc taaccaagtg accaaagcat ctcccgcaag
    21721 cttacctaac tgttcaaaat cttgtgcgat ggttgtcagt ggcgggtcaa caagagctgc
    21781 tgcttggata tcatcaaatc cgataattga aaaatcttca gggatatgat aaccatgctg
    21841 tctcaagaga ttcatggtct ggatagcgac aacatcgttt tcacaaacaa aagcagttac
    21901 ttcatactta gtgatataag aaagcaattc atctaatgtc gcgcttttgt catccagtga
    21961 agtgtggaaa gtcaaaccag ctttgtccaa tgcttcaagg taaccaaggt aacgctgtcg
    22021 aacggattgt ggatgagtct gcgtaccaaa caaataagcg atttttgaat gtccctgttc
    22081 gatcaaataa gaggcagcaa gcgcaccgcc ttggaaatta tcggaaagaa cggtaggaaa
    22141 aggtaactca tagatttttt tatctaatat gacgacaggg aattctttca atgaaagctc
    22201 aaaaagtaag tcaagatgtt gttctgtatc aagagcataa taaatcaatc cgtcaaattc
    22261 attgattagc tcttttgctg ttttattttg tagaaagtca agcgtcgtca tcatgacatc
    22321 aatctgttta tcttgcatca cagggttcaa tcccttgcta aaatcaccaa cagacaaatc
    22381 agcgacatat ggaagaagaa acagaatccg tcgtgttttt gtaggagaag caagaatctg
    22441 ttccggagaa gcggatttaa cgaaactgcc tttcccgcga acgcgataaa taagtcctaa
    22501 ttgctctaat tcagtcaatg cacgtttgga agtgattctg cttactttat aggtacgtga
    22561 aagttctttt tcagttggta gttggctgtc tacaggtaat tcaccagtta aaatagccgt
    22621 ttgcaaatca ttaaatattg tctggtacaa tggttgattc atataaacac ctcttaatga
    22681 tatatcatat tcaaataata ccataatgga tgaaaaagta aataaaaaga tattttttga
    22741 aagcgtagac ataattggct gtatgaagta taatctttaa agatatatca aatagaaaga
    22801 aggttttatt agtggcttat aaagatatac caaaatcggt gcagaaattt atggaagata
    22861 tcacacaaaa atgtggggat atccatgcgg actgggcaaa gaattttaca gcagcatttg
    22921 ccaatactct gttgacaaca gtcaaaaagc aaaatgacgg aaccaccttt ttattgacgg
    22981 gagacattcc agcgatgtgg ttaagagact ctactgcaca agttcgtcct tatttagtga
    23041 ttgcaaaaga agatgaggaa ttagctgaca tgatcagcgg gttagtcaaa aagcaatttt
    23101 tttatatcaa tatcgatcca tatgcgaatg ccttcaacga agaagctaac ggtgctggcc
    23161 accaaacaga tcatacaaaa atgaacgatt ggatctggga aagaaaatat gaaattgatt
    23221 ctttatgcta cccaatacag ttggcctatc ttctttacaa aaatacagga aaaactgatc
    23281 aattcaatca atcttttatt gaaggtatca aaaaaatctt ggacgtgttc aaaacagagc
    23341 aagatcatga acactctcct tatacatttg tacgagacac gacaagaaaa gaagatacgc
    23401 tgatcaatga cgggcgaggt gcaaaaacag cttttacagg aatgacatgg tctggtttcc
    23461 gtcctagcga tgatacttgc cgttatggat atctggtacc atccaatatg tttgcggtgg
    23521 tcgtgttagg ttatgtggaa gaaatttttc aaacgatact cgatgataaa gaaattgtac
    23581 agcaaacaca gaaattgaag aaagagatcc aagaggggat tcagaatcac ggacgaacgg
    23641 ttaatcaaaa agggcaaacg atttatgcat atgaagtaga tggaatggga aacgccacag
    23701 tcatggatga cagtaatgtg cccaatctga tcgccgcacc ttacttggga tattgttctg
    23761 aacaagagga agattatctg caaacaagaa gaacattgtt gagtaaagaa aatccatatt
    23821 tctatgaagg aaagtacgca aaaggaattg gatcctccca tacgccagaa aaatacgttt
    23881 ggccgattgc attagctatg gaaggaatga caacgtcaga caaatctgaa aaagagcgga
    23941 tattgaattt acttgtagca actgacgcag gaacacacct tatgcacgaa ggattcgatg
    24001 tgaacaatcc tgaaaattat acgagagaat ggttttcttg ggcaaatatg atgttttgcg
    24061 aattagtcat ggattacttt gatattagag tagaaaagta ggaggtatcg taaatgaaaa
    24121 agaaagtata tatcatttct catagccact gggatcgtga atggtacatg gcttacgaac
    24181 agcatcacat gcgactgatt gaattgatag acgatctact agagctattt gaagttgatc
    24241 cgtccttcaa cagttttcat ttggatgggc aaacgattat tttagatgac tatcttcaag
    24301 taaggccaga aaaaagacag gcaatccaac aagcaatcaa tgaaggaaaa ttgagaattg
    24361 gaccatttta tatcttgcaa gatgattttc ttatcagctc ggaatcgaac gtgagaaata
    24421 tgctgattgg catggaagaa agccgtaaat ggggaacccc tgtcatgtta ggctattttc
    24481 ccgatacatt tggaaatatg ggacagacgc ctcaattaat gaaacaagct ggtatttcag
    24541 cagcagcgtt tggccgtggt gtaaaaccaa ttggctttga taatcaagtt ttggaagcag
    24601 aaaactattc ttcccaatac tcagagatgt ggtggaaagg acctgatcaa acagcaatct
    24661 ttggtctgct atttgccaat tggtatagca acgggaatga gattccagta gaaaaagaag
    24721 cagctcttgc cttttggaag caaaaattag cggatgcaga acaatatgcc tctacgaacc
    24781 acttattgat gatgaatggt gttgatcatc aaccggtaca aaaagatata tcaaaagcaa
    24841 tccatctcgc aaatgaactg tttccagatt acgagtttat tcattctaat tttactgact
    24901 atctggaagc agttcaaaaa gatgtgccag aagacttagg ctcagtagaa ggagaattga
    24961 caagtcaaga aacggatggc tggtatactt tagccaatac agcatctgct cgggtttact
    25021 taaaacaatg gaataccaag gtccagcgac agctagaaaa tattacagaa ccacttgcga
    25081 cgatggctta tgaagttagt ggaaactatc cccatgatca attagattat gcttggaaga
    25141 cattaatgca aaatcatcct cacgacagta tttgcggttg ttcagtcgat tctgtccatc
    25201 gggaaatgat acctcgtttt gaaaaagcag atgaagttgg aaagtatctt gcacaagatt
    25261 ccttagaaca gctgaccgca gcaatcgata cgaccggatt cccaaaagac agctttccat
    25321 ttgtcattgt aaatactgca ggaatggata aaactggtga agcagaaata accatcgaat
    25381 tagaaagaaa aagattcgct gaaggaatac ctgaacaact atatcaagag ttagaaaatt
    25441 tgcctaaacg taagtatcat gtagaaacaa aaagtggggc cactatacca gctattttat
    25501 ctgaagaaac tgtccaattt ggttatgatt tgccgaaaga ccgtttccgt gtgccatata
    25561 tggcaagaat gatcaaagtg actttaccat tagaaaacat gccagcattt tcttgggaaa
    25621 cattcgcgct tgtagaaggg gaagcgaaag cagaagaaaa ggaaacaatg attcatcaga
    25681 gtggacgaat catcgaaaac ggccctctac atttgacgat tgaaaaaaat ggtaccatta
    25741 caatggaaga tcgcaaaaac aaacgaaagt tgaataatct gcatattttt gaagatatcg
    25801 gcgatatagg aaatgagtat atctttaaac aacctttctg tgatcagcca atcctatcca
    25861 gtaacaaaga aaactctgaa gtaaaagtat tagtagatac accggaaata gcaaaaataa
    25921 gtattctaca ggaaatggaa atccctgttt ctgcagacga gcgtttagaa aaagaacagc
    25981 agatggttgt ggaatttcgt tatcgaaaag cagaacgatc caaagaaaaa cgaatccttc
    26041 aaataaaaac aatcatgaca atcagaaaag atagtaaaaa aatcgacttt gaaacgactc
    26101 tcgacaatca gatgaaagat catcgcttac gtgtcttgtt tcctacaaag ttacatgtgg
    26161 aacatcacga agcagatagt atttttgaag tggtgaagcg cccaaaccat gtgtctaaaa
    26221 gttgggaaaa ccccacgaat ccgcaacatc agcaagcatt tgtcaatatt catgatgaag
    26281 agtacggtgt aacagtcgga aactttggtt tgaatgaata tgaagtaaca gaagacggac
    26341 aaatcgcagt gacgctactt cgcagtgtgg gagaactagg cgattgggga tattttccaa
    26401 caccagaagc acagtgtcta ggtgagcatc ggttcaatta cagcattgaa ctacatggac
    26461 cagaagaaaa attctctact tatttgcatg catacgcagc acagattcct ttttccactc
    26521 aacaaatcaa acatcatgaa ggaacattga tttcaaaaca acaatattta acgataaaaa
    26581 gtgaaacgtt tgcaattaca gctttgaaac gcagcaagtt tagtgataaa gtagtagtaa
    26641 gaggtttcaa tatgtctagt catttagaaa aattggaaat aactaaagac aatggaaaaa
    26701 ctgtcatatt gaatctgctt gaagaaccaa cgaagcaagc ggttgtccca ataatccaac
    26761 cttatgagat tcggacaatc ggattcgagg aggaaaacga atgtatggcg gaatagaagc
    26821 aggcggaaca aaatttgtgt gtgcggtttc agatggtgga gaaatttta
//
LOCUS       DACDIE010000036        25743 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.036, whole genome shotgun sequence.
ACCESSION   DACDIE010000036 DACDIE010000000
VERSION     DACDIE010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25743
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.036"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(49..1281)
                     /locus_tag="IV250_001655"
     CDS             complement(49..1281)
                     /locus_tag="IV250_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP8768942.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1535..2104
                     /locus_tag="IV250_001656"
     CDS             1535..2104
                     /locus_tag="IV250_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8768943.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2282..2722)
                     /locus_tag="IV250_001657"
     CDS             complement(2282..2722)
                     /locus_tag="IV250_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAP8768944.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2880..3644
                     /gene="map"
                     /locus_tag="IV250_001658"
     CDS             2880..3644
                     /gene="map"
                     /locus_tag="IV250_001658"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAP8768945.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3676..4599
                     /locus_tag="IV250_001659"
     CDS             3676..4599
                     /locus_tag="IV250_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAP8768946.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            4675..5814
                     /locus_tag="IV250_001660"
     CDS             4675..5814
                     /locus_tag="IV250_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HAP8768947.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            5807..6607
                     /locus_tag="IV250_001661"
     CDS             5807..6607
                     /locus_tag="IV250_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP8768948.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            6607..7434
                     /locus_tag="IV250_001662"
     CDS             6607..7434
                     /locus_tag="IV250_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP8768949.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            7412..8146
                     /locus_tag="IV250_001663"
     CDS             7412..8146
                     /locus_tag="IV250_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP8768950.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            8246..9112
                     /gene="rfbA"
                     /locus_tag="IV250_001664"
     CDS             8246..9112
                     /gene="rfbA"
                     /locus_tag="IV250_001664"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAP8768951.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9126..9698
                     /gene="rfbC"
                     /locus_tag="IV250_001665"
     CDS             9126..9698
                     /gene="rfbC"
                     /locus_tag="IV250_001665"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAP8768952.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            9720..10748
                     /gene="rfbB"
                     /locus_tag="IV250_001666"
     CDS             9720..10748
                     /gene="rfbB"
                     /locus_tag="IV250_001666"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAP8768953.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            10846..11697
                     /gene="rfbD"
                     /locus_tag="IV250_001667"
     CDS             10846..11697
                     /gene="rfbD"
                     /locus_tag="IV250_001667"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAP8768954.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            11732..13765
                     /locus_tag="IV250_001668"
     CDS             11732..13765
                     /locus_tag="IV250_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8768955.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            13809..15089
                     /locus_tag="IV250_001669"
     CDS             13809..15089
                     /locus_tag="IV250_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAP8768956.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCRSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVTEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            15299..16105
                     /locus_tag="IV250_001670"
     CDS             15299..16105
                     /locus_tag="IV250_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8768957.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            16117..17337
                     /locus_tag="IV250_001671"
     CDS             16117..17337
                     /locus_tag="IV250_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8768958.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGKVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            17327..18913
                     /locus_tag="IV250_001672"
     CDS             17327..18913
                     /locus_tag="IV250_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8768959.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLYINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAQPQNSNYVKVLEVIQQTKQDEIIQQIPFNNFTGENETLSFHVSELA
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKDLPEFFLTDIAG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHYLIENNTRLD
                     QTLKTTINRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSLCIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVLREEVNQAKQLAEGAKAGFELRILCEK
                     ERSFLVEAVAQNGQSWIIEM"
     gene            18952..21090
                     /locus_tag="IV250_001673"
     CDS             18952..21090
                     /locus_tag="IV250_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP8768960.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEVQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRQENGHISLATNSALEMAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDLIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPAEHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVHPGRISGFYEITYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYPNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTAMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(21458..22441)
                     /locus_tag="IV250_001674"
     CDS             complement(21458..22441)
                     /locus_tag="IV250_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HAP8768961.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            22819..24210
                     /locus_tag="IV250_001675"
     CDS             22819..24210
                     /locus_tag="IV250_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP8768962.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            24226..25266
                     /locus_tag="IV250_001676"
     CDS             24226..25266
                     /locus_tag="IV250_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP8768963.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            25285..>25743
                     /locus_tag="IV250_001677"
     CDS             25285..>25743
                     /locus_tag="IV250_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAP8768964.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEA"
BASE COUNT         8179 a         4342 c         5232 g         7990 t
ORIGIN      
        1 ctttattcta tgctttatat tattttttgc atgtgtgcga aagtatactc aagcccaatc
       61 gccatttcgg aaaatcggta ctgtagagcc atcttcacgg attccgtcga tatccatctt
      121 gtcagatcct accatgaaat ctacgtgggt ttgacttcta ttcaatccag cttctgctaa
      181 ttcttcatcg gacatttctg taccgccttt cacactgaag gcataagcag aacctagtgc
      241 taagtgattc gatgcgtttt catcaaataa tgtattgaaa aagatgatgc cagattgaga
      301 aataggtgaa gggtcaggta caagtgcgac ttctcctaaa cgacgcgcac cttcatctgt
      361 atcaagcagt tttgctaaga catcttctcc ttgttcggca gaaaaatcaa caacttttcc
      421 atctttaaaa gtaaatttca tacccgagat gatggtgcca gcataactca gtggctttgt
      481 acttgaaatg taaccatcta ctcgatgact gtcaggtgct gtgaacactt cttccgtcgg
      541 catatttgcc atgaattttt ctccacgagc attatagctt ccagcgcctt cccaaagatg
      601 gtttttaggc aatccgataa tgatatctgt accgggagca gtatagtgaa gggcagaaaa
      661 ttgttctcga ttcaattctt ctgctttttt tgctaatttt tcatcatgct tcttccatgc
      721 taagacaggg tcttcttcat agacacgagt cgttttgaaa atctgatccc ataatgcctc
      781 aacttgtttt tcttctggca gttctgggaa gacttttgct gcccattgtt taccagccgc
      841 agccacgaca gtccaactga ctttatttgc ttgagttgcc ttgcgtaaat tcattagtgc
      901 tttgccattc gctgattgat aagacgctac tcgatggcta tctacaccag caaatgcatc
      961 gggatcggca gaaacaacgc tgatcctgct tgctcctttt tcaagccatt catccgcttg
     1021 atcgatttta gattgtggta cattctcaat acgatccgtt gcagcatgta aaagaaattc
     1081 tctttggatc tggtcgtcag tccattgaac gataacctca gcggctccta gtttataagc
     1141 ttcttgtgtg atcaatcgag caaggggtgc ctgttccacg ctgatctgta aaacaactgt
     1201 gtgtcctttt tctgtggcta cgccagtttc tgcaattaag cgtgcatatt tttttagaag
     1261 ttcattaaaa tcagataaca taataaaaat tcctcctcac cattttttct ggtaactatt
     1321 agcatgatac catttaatta tgattcaaac aatgaggctt taaataaaaa aaggaggtta
     1381 tttttttaag gttaagtttc atgatatatg ttactacttg ctatttcagc ttgtgaagaa
     1441 aatcaaaaac taattttgca tatcaaaaaa acatgttaca atgaaattaa tcaatggtgg
     1501 aagcgttata aaaaaattaa ggagattaaa aaatatggca agaaaaaaga cgattactag
     1561 agaacagatt ttggcagctg cttatgaagt agctgccacc gatggattta cacgatttac
     1621 tgcgagaaac atcgcgaaca aaatgaattg ttccacgcaa ccgatttatc ttgagtttaa
     1681 aaatatggat gacttgagac aagctctttt tgagcaaatc tatgatcatt tacaatatga
     1741 agtgttcact gttgagcata cagggaatac gattatcgat cttgctttta attatatcca
     1801 ttttgcaaga agagagaaga agttgtatcg ttcactgtac ttagaagaat atggtggagg
     1861 aaaagagatg cagaatttct cttatactta ctttgtgaat acagtgaaac gcgatccagc
     1921 ttatgtgaat ctttcagaag agcagatcga atctttgtat aatgggacat ggatcatagc
     1981 tactgggatc gcagctttga tgtcttcaag catcatccat ccaactgatg aacagattga
     2041 aaagatgatc caagacagta tagatgctat cttgaaattc aaagaaccaa tcgaaatcga
     2101 ttgaatatac tcaaaagttc cacaaatcag taggtaacaa gagatgtgtc aaaataagga
     2161 aaagttcgtt tttcttacag cgtatgcatt tttaactccc gaagaactct tccatgaatg
     2221 caatgaataa aagagccata cagattgaat aaatctggat ggctctttta ttgcaagtat
     2281 attaaatttt tgcgactagt tctttagcga aagcttctaa attttcgata tcttcttctt
     2341 cagcattcag atccacttta acattttcgg ctccttttga tgcgcctgtt tttgcaaaag
     2401 cagcatcgaa atcatccacc gatttgcaga attcatcgta aaaagtatca ccagatccgc
     2461 agacaccgta aatttttccc aatagatcca attcttgcaa gtcttcataa aagtctacga
     2521 tctcatcagg cagatcgcca tcatcgtatg tataagtagc tactacgcaa atatctgcct
     2581 cctcgaattc atctgcatct acttgggtac attcgttgat ttcgacttcg atatccagat
     2641 tttcaaatgc ttctgcaaca atatcggcga tttcttcagt gtttcctgtc atgctagcat
     2701 atacgatttt tgctaaagcc atgatgtgcc tcctcgttta taattattta acaattatga
     2761 attataccaa aacatcattg aaaagaaaat aaaacctaaa gacacctggg gaatgtcatt
     2821 tggctaaatg tatgccgcta tgttaaaatg agagggattt gtaaaaagga gacgaaaata
     2881 tgattacttt aaaatcagca cgtgaaattg aaatgatggc agaatctgga gctttattag
     2941 cagatgtaca taaaaatctg cgtgatttta taaaaccagg aatcacaagt tgggatattg
     3001 aggtatttgt cagaaactat atcgagagtc atggcggaat tgctgcgcaa attggctttg
     3061 aagggtatga atatgcgact tgtatcagta taaatgatga aatttgtcat ggattccctc
     3121 gtaaaaagcc tttgaaaaac ggtgatttgg taaaagtcga tatgtgtatc gatttgaaag
     3181 gcggcatttc tgattcttgt tgggcctatg tagttggaga atctacacct gagatcgacc
     3241 gattgatgga agtaacgaag aaagctttgt acatcggtat tgagcaagca caagtaggga
     3301 atcggattgg tgacatcggt catgctatcc aaacttatgt agaaggcgaa aatctagcga
     3361 tcgttcgtga ttttattggt catggcgttg gtcctacgat tcatgaagaa ccagcagttc
     3421 ctcattacgg agaagctgga aaagggttgc ggttgaaaga aggcatggtt atcacgatcg
     3481 aacctatggt taataccgga acttggaaaa tgaaaatgga tcctaacggc tggactgcgt
     3541 atacaagaga cggcggactt tcttgccaat atgaacatac attggctatt actaaagatg
     3601 gtccaagaat tttgacatca caaggagaag aaggcactta ttgatctatc cagtcaatag
     3661 gtacgggaga ggtacatgaa agtttggaac aaaataaaac aaaatgaaaa tctgatgcgc
     3721 tttattgaga cgactcaaaa gcgcatcact gattcagaaa tgagtacgac ctctgtcgtc
     3781 gttgcttatt atttactctt gtctttattt ccattgatta ttgcttttgg taatatttta
     3841 ccgtttctcc acatcgatca agagactgta ctgacctatt tacgagaagt gattcctgag
     3901 accgtttacc agtttatcgg tcctgcaatc aaagaccttt tgacacaaag ttcaggaggc
     3961 ttgttatccg tttctgcatt agcggcttta tggtctgcaa gtcaaagtat caatgctttg
     4021 caaattgcga tgaataaagc gtatggcgta gaaaatagaa aaaatttttt gatcgtccgt
     4081 ttcttctcac taattgtcat tgtcttgttc atgattgcta ttagtggggt aactatcgtt
     4141 cttgggttgg gacagctgat tttggatgct attcagccaa tcgtacaaat acccgtgaac
     4201 tttatcgatc agttccaaac tttgaagtgg ccaatcacat tggtagcttt atttgtcatc
     4261 atgttcttga tttatttaat tgtccctaat gctcagttga agttgaagac gatcattccg
     4321 ggcacgatct tcgctactgt cggctggatg ctgttatcac aagtgtttag tatctatgct
     4381 aggtattttg caacgaggat cagcggttat cagatcatag gaagttttat tgtactcatg
     4441 ctttggctta attttgctgc tacgatcatc attttgggcg gaatcatcaa tgcagttgtt
     4501 caagaatacg tgacgggtca tcaaatcaaa aaacgtcgag atccaagccg aaaatggttc
     4561 aataaattga aaactaaatt ttctcataaa aaaaagtaga aacaagagtt cttattgtaa
     4621 atttatatca gtaatttata ataattatga ctcttaatta aagtaggtgc ccatatgctt
     4681 gtttctttta gtatggttat aagatttttt ctgacaattt tattatcact gatattaaca
     4741 cctgtattca aaatcatttc agtacaaaca ggtatggtcg acaaaccaaa tgaaagaaga
     4801 ataaataaag ttccaatgcc ttcagcgggc ggactgccta tctttatttc atttgttata
     4861 tcgactttat tcttattccg taaaattatc cctcaatcgt atattgtacc gatcttgatt
     4921 gcttctttag taatcatagt cacaggagtg ctcgatgata aatatgagct gacacctaaa
     4981 caaaaaagta tagggatctt gattgcttct cttattattt attttgtagc agatatacgt
     5041 attgattcct tcacgcttcc ttttattgga tatatccaac taggctggct tagttttccc
     5101 gtaacgattt tttggatctt cggtatcacg aatgcggtca atctgattga cggtttggat
     5161 gggctggcgg cagggatttc attgatcggt ttaattacga taggaatcat tggatatttt
     5221 ttcctccatg cttctacagt gtatattcca gttgttattt tctgtcttgt agcaagtatt
     5281 atcggttttt ttccttacaa tttttatccg gcaaagattt atttaggcga tacaggagct
     5341 ctattcctag ggtttatgat ggccgtcctt tcactacaag ggctgaagaa cgtgactttt
     5401 gtttcatcga tttctttgct gatcgtcatg ggagtgccgg taacagacac gttttttgct
     5461 atcattagga gaaaagcaaa ccgtgtttct ttttctacag cagataaaaa acatcttcat
     5521 caccgactaa tcagtttagg ctttactcat aaaggtgctg tcctgacgat ttatgcgatg
     5581 gctttgatgt tttctttcac agctatggtc atgaattata ctggaagaat cggaacgatc
     5641 atattgatta ttgccatgtt gtttgctgca attttattat ttgaattgat tggtctgatc
     5701 aatgagaaac atcagtttat tttaaaaaca ttgcgatttt tagggaataa agagtaccgt
     5761 acgcaagtaa aacagaaata tttgaagaaa tggaaccgtc atatagatga ataacaaatt
     5821 aaaccagccg atccttatta gtattgtgac ggcaaacagt gataaaattt ttcggacact
     5881 agataatttt atttcaactg taaaagatga aacagcagta aaaatccgaa tttttgacaa
     5941 caattcactt cccgagtaca aaaaaagact aacagagtat aggaaatatc cctacatcga
     6001 tatctatttt catgatgaaa attccggatt tggttatgga cataatcaca atctgatagc
     6061 tagcgattct cgctatggca ttatttgtaa cccagatatt ttagtggatg aagagacagt
     6121 tacagcattg gtcttgttat tgaaagaaca tccggaatgt gcgatgttag cacctaagat
     6181 tcttaatgaa gatggaacaa ctcagcacct catccgtgag cagctggctg tttttgatta
     6241 cgttttgcgc tttgtgcctt tcaaatgggt caaaaaaaag tttgacaaac gactggctgt
     6301 gtttgaatgc cgaggattgc ctactgatgc taatagttat gtacggatga tctcaggtag
     6361 ttttatggta gctgatattg ataaatttaa agagataaag gggtttgatg agcgtttctt
     6421 catgtacttt gaagacaacg acttgtgttt gagttttgaa aaagcaggtt acaagttatt
     6481 gtacacaccc ttttatgcag ttatccattt ttacgggaag ggtgcgcatc gaaatgcaaa
     6541 actgtttcgc gtgtttttga cttctatgat gaaatttttc aataagtggg ggtggcgttt
     6601 tttctgatga aaccagaaat attagtagtc gttgtgcttt atcagcaact attcagtcaa
     6661 tctccagctt atggaccatt aagcaaagca cttagcgaaa agcaaattca gcttattatt
     6721 tacgataata gtcctcaaag gcagcaaaat gagctgtttg aaaaagaaaa tatcgtttat
     6781 catcatgatc cgcaaaatcc tggtttagcg gtggcttata attttgcgtt gtctcaagca
     6841 gatgaaggaa cacgttgtct cgtcactctg gatcaggata cgaggctagt cgacgattat
     6901 tttgaactat tgcgtaaagt aacctttaca gatgaatgtg ttgctgcagt tccgatgatt
     6961 ttttctggga gccgacaagt ttctccccta tattcagatc gttatatcaa tcgtacagct
     7021 caagcagtag aagttgaagg cccaacagct gaacgaatca tggcaatcaa ttcaggtgtt
     7081 gcctggtctg tcaaatttct aaaaaagatt ggcgggttta atcctgcttt ttctttagat
     7141 tttttggatc attggctgtt ttggaaagtg gatcagttgg aaaaaactgt ggagatcctt
     7201 cctgtacgaa tggaacatga tctttcggtt ttagactatg aaaaagtttc aagtaatcga
     7261 taccgttcta ttttacaagc agaaaatcga ttttatcatg aatatgatca aaggcattta
     7321 tccagtcacc gacgacaatt attgttaaga acagctaaac agttcttgac cgtcaaaaac
     7381 cggcatatct ggcggatgac tttgcgctca tatgttaaga attggaaggt gtgaattcgt
     7441 gcgttcggtt tgtatagcta cctttaatgg aggcagatat ttaagagagc agttagatag
     7501 tattttatct cagctttcag cagaagatga agtagtcatt tctgatgatg ggtcagtaga
     7561 tgatacatgg gatatattaa ctgaatacgc agaaaaagat aagcgaatcc agcttttttc
     7621 ggggcctcgt caaggattga tcgctaattt tggttatgcg atccagcaga cgaaaggaga
     7681 tctaatcttt ttggcagacc aagatgatgt ttggttggag acaaaagtga aaaagatcac
     7741 tgactatttt gagcagaatc cggatcagct agtagtagtt tctgatttaa tcattgtgga
     7801 cagcgagctg caagagatcc atgattcgta tttttcgttt cgaaattcca aagaaggatg
     7861 gcttaataat gtgataagaa gtcactatat tggtgctggt atggctttcc ggtcttcttt
     7921 aaaacccgat attttgccga tcccgtcaga agtgccgatg catgatatgt ggattggttt
     7981 gctgggagga aagaagactg ggttcctaag agaaccgtta actctgtacc gaagacatga
     8041 tttaaacgcg agtgagattg aaacacattc taaattacgg caaaaaattt cttggcgtgt
     8101 tcatctgatt acggcgttga tcaaacgaaa atggtttaaa cgttgataaa agatgtttaa
     8161 atccttaata tttccatgac aaatataaca attgatgaga taataacaaa gtgaaatttg
     8221 aaatcaaaaa ggagagcaga tatccatgaa gggaatcatt cttgctgggg gcagcggaac
     8281 acgtctgtac cctttaacaa aggcaacatc gaaacaattg atgccgattt atgacaaacc
     8341 aatgatttat tatccaatgt ctactctgat gttggcggga atcaaagaaa ttctgattat
     8401 ttcaacgcca caagatacac cacgtttcaa ggaattattt ggaaacggaa atgatttagg
     8461 actgcagatc gaatatgcag tacaagaaag tccagatgga ttggcacaag cgtttatcat
     8521 tggagaagaa tttattggag atgatagtgt ctgcttggta ttaggggaca atatttacta
     8581 tggtggcggt ctgtctaaaa tgttgcaacg agcagcttca aaagatacag gcgcgacagt
     8641 atttggctat catgtcaatg atccagaacg ctttggtgta gtagaatttg atgagaatat
     8701 gcaagctttg tcgattgaag aaaagcctgc tcagccaaaa tcaaattacg ctgtaactgg
     8761 cctttatttt tatgacaatg aggtaatttc tatcgctaaa ggtatcaaac cttcagaacg
     8821 tggagaacta gagattacgg atgtcaataa agcatatttg gataaaggaa aactatccgt
     8881 agaacttatg ggacgcggat ttgcttggct tgatacagga acacatgaat cattgcttga
     8941 agcatctacc tttatcgaaa caattgaaaa acgtcagaac ttaaaagtgg cttgtttgga
     9001 agaaattgct taccgtatgg gctacatcga taaggaacaa ttgctggccc ttgcacagcc
     9061 attgaaaaaa aatcaatacg gtcaatattt attgaatttg gccgctgaat aaggagtcct
     9121 tacagatgaa agtaacagat acaaaattaa cagatgtcaa aatcgtagaa atggacgtct
     9181 ttggcgatca tcgcggtttc ttcacagaaa gttattcaaa agcaaaattt gcagaacatg
     9241 gattgaaata tgactttgtt caagacaacc attcactatc agctgaagct ggtgtccttc
     9301 gggggcttca tttccaaaaa ggagaagctg cacagacaaa attgattcgg gtagtcactg
     9361 gagcagtttt agatgtagta gtggatatcc gcaaaggtag cccgacttat ggtcaatggg
     9421 acggatttat cttatctgaa cataatcatc gacagttgct tgtacctaaa ggatatgccc
     9481 atggattcgt tactttgaca ccaaatgtga acttccttta taagtgtgat aattactata
     9541 atgcagaagc agatgggggc atcgcatttg atgatccaga tttagcgatt gattggccaa
     9601 ttgatccatc aagagcgatt atgtcggaaa aagatcaccg tcatccgaca ttaaaagaat
     9661 ttgaagcaga aaatccattt gtttacggag aaatctaaga attaaggaga aattttctta
     9721 tgaaaaacat cattgttacc ggaggtgccg gattcatcgg ctctaatttc gttcattacg
     9781 tggtaaacaa tcatccagaa gtgcatgtca ctgttttgga taaactgact tatgcaggaa
     9841 acaaagagaa cttggccgga cttcctagcg atcgcgtaga attggtcgta ggagatgttg
     9901 cagatgcaga attagtggac cgtctagtaa aagaagcgga cgcagttgtt cattacgcgg
     9961 cagaatccca taacgataat tcattaaaag atcctttccc atttgtacaa acgaatatca
    10021 ttggtacgta tacactttta gaagcttgcc gcaagtatga tgtacgctat catcacgtat
    10081 ctactgacga ggtttacggc gatcttccat tacgtgaaga cctgccagga catggggaag
    10141 gcgaaggaga aaaattcact gcggaaacac catataatcc ttcaagtcca tattcttcta
    10201 caaaagctgg ttcagatctg ttagtaaaag catgggtgcg ttcttttggc ttacgtgcaa
    10261 ccatttcaaa ctgttcaaac aactatggtc cttatcagca tattgaaaaa tttattcctc
    10321 gccaagtaac gagtatctta agcggtattc gtccaaaatt atatggcgaa ggaaagaatg
    10381 ttcgtgactg gatccatacg aatgatcatt cttcagcagt gtggctgatt ttgacgaaag
    10441 gcagaattgg cgaaacctat ctgatcggtg cagacggtga agaagataat aagaccgtga
    10501 tggaattgat cttagaaatg atgggccaac caaaagacgc atacgaccat gtaaacgacc
    10561 gcgcagggca cgatttgcgt tatgcaatcg attcgacaaa attgcgagaa gagctaggtt
    10621 ggcagcctga gtttacgaac ttccgtgatg ggttggctga cacgatcaaa tggtacgaag
    10681 aacatgaaga ctggtggaaa aaagaaaaag aggccgttga agcagcatat gccaaaaatg
    10741 gacaataata cagaggaaat ttcactgtat taatggaaaa acacatgtga ggcaggcgct
    10801 cgcctgtctc ttttttaaaa taaaaatcaa ggagcggata accatatgtt tttattaact
    10861 ggaggaaatg gacaattagg tacagaattg cgccatttat tagatgaaaa aaatctgcca
    10921 tatgtttcaa cagatgcaaa agacttggat atcacagatg ctgagaaaac aatggcttat
    10981 attacagaat taaaacccga tgttattttc cattgcgcag cttacacagc agtagataaa
    11041 gcagaagaag aagcaaaaga attagatgag aaaatcaatg tcgatggcac aagaaatgta
    11101 gcacttgcag caaaagctgt gaatgcacaa ttcgtctata tcagtacaga ctatgttttc
    11161 gatggaaaaa acaaaaatgt agaatacgaa gtagacgacc aaacgaatcc actaaatgaa
    11221 tatggtcgta cgaaattatt aggcgaaaaa gcagtagaag agattttaga agactattat
    11281 atcatacgaa cttcttgggt tttcgggatc tatggacaca atttcgtctt cacgatgcaa
    11341 cgcctagcgg aaacccgcga caaactgact gtagtaaatg accagtttgg gcgaccaact
    11401 tggacacgta ctttggcaga atttatggta tatgtcatcg aagaaaaagc accatttggt
    11461 atttatcatt tgtcgaacga aaatagctgt tcttggtatg agttcgcaaa agaaatattg
    11521 aaagatacag atgtagaagt tgcacctgtt acgtcagaag aatttccgca aaaagcaaca
    11581 cgtccgcagt attcagtcat gagcctgaaa aagacagaag cgttaggttt tgtcatacca
    11641 acttggcaag aagcacttgc gcaaatgctg gaagctgcca aaaaaatgaa taagtaattt
    11701 cctttttatc aaatagaatt tggagcatga tatggaaaaa caaacaagaa gtgcacgacg
    11761 aaaagaaaag ccaaaagcga atcaggcaga ttcgaatttg gtgcttgacc tgtttctaaa
    11821 actccgctat cttattggcg ttttagtcat cgtggtcatc gttactttta atttaaacgg
    11881 aagttcactt ggtgtttggg ataaatatgt gtcgcaaaga gacgatggga aaaaaactga
    11941 tgtgattttt ggacagaatc gggaagtgcg gtcagacgaa tggctggtgc agacaccttt
    12001 ttacctctca caagctgaag aggacttttc gttggtgaac gaggattatt cattaaacgg
    12061 acaaaacatg atcattgcgt ataattctcc tgtaaaggat attacggtta tcggaaaacc
    12121 attcaactgg ggattctttt tcctcggaag agatcgagga ctttcctttt attgggcgat
    12181 gaaattagtc gtcatggtct tgcttggatt cgaagtactg atgatattaa cgaagaggaa
    12241 aaaagctcta tcgctaatcg gtgcatttgc gctgacattt tctccagcag tccagtggtg
    12301 gttcatgcaa catgtgggcg atttgatttt ctttactttg ggactgatga ttgcttttta
    12361 tcattatttc tacaatcatg ataaaaaatg gctacgaacg ttgatgatgc ttttggtggt
    12421 tattttcggt attgggttta ttttagtgat ttacccagcc catcaagtga tgctggcgta
    12481 cttgctgatt ttttattttg ttggcctgct tatttactat agcaagaaag tttcatggga
    12541 tctgttggat gtagtgttga ttggaggggc agttttattt attggcggag tcatggttca
    12601 tttctggcta acgtcaaaag acgcattgtt agcttctttg aataccttat atccaggaaa
    12661 acgggtaagt actggaggaa agtggacgat tggcgaattt ttttctttcc ttacaaattg
    12721 gaaaattcct tttgaagata tcaactattc gaataactct gaagtggccc tgttctatca
    12781 cttttttcca actgtattcc tagcaagtcc atttgtgtta tttggcaaaa aagatagcga
    12841 acaaaagtta ctgggacgta ttttgatgat tttttgtctg ttttctatct tttggattac
    12901 cgttgggttg ccaaaatggc tagcagaaat cactctgctg tcttatgttc ctccattaag
    12961 agccatcctt acttttagtt ttgcggcttg tttattatcg atctggttta tcgcttatat
    13021 ttggagagaa gatgttattc ctatttggta taaattcatt ttgttggcag taaatgcatg
    13081 gtcttatttc tatgcgatta cgcattcgaa aatggataat tatttttcgg attttgaaac
    13141 catcgcagtt gctgtgttgg gaacagtgat tttagctttt gcattatttg gctggagacg
    13201 cttgttttac ctcgcttttg tcgccttagt tatcgtatct ggctttttcg tcaatccagt
    13261 cgtagaaggt acaggagcga tttttgaaaa aacactggca aaagaaatcc aaaaaatcga
    13321 tcaacaagat cctggtaaag tttggttgtc tgaagatgaa ttatataact tcaccccaac
    13381 attgggagtg aaatccttca atacagtacg cttttatcca gatatagctg catggaagaa
    13441 aatcgatccg aaagaagaat acgagaagta ttataatcgg tatgctcata caagagcttt
    13501 tatcgctaag gaagatacaa agttcatttt agatcgtcca gatatgtttt cggtcacgtt
    13561 gaattttgaa gacgcagaaa aattagggat cgactatgtg atcagtaaac gaccattaac
    13621 tgattacaac caattatatg aagcgcagtt cacgcagcta tatggaccgg ataaagacgg
    13681 ttatatgatt tttaaagtag aatatccgga ttatccagat gtaccacaaa cacaaactca
    13741 ggaaaatcag ctagtcgtac agtaataggc tagctgagaa taatcctgaa atattagtag
    13801 aaaggagagt gaagataatt caagcaatcg tacaaaaatt tgtcaacaaa atgagtgtcg
    13861 gtttcttgct tttgttgctg acgctagtct tttttgtttg gacagcaggg gtaggttttc
    13921 cagcaacttt gtttatatat aacaatagtt cattacttat cgcttttagt gtggctattt
    13981 tgctcatttt aaatgtttat aaagcaaaag gcgtcgattt tctttatcta ggtgttgcgc
    14041 ttgccctttt cattttcttt tcttactttc gggaattaag aaatgagtct ttatttggag
    14101 acttactgtt tcctttgatt attttatttt gtttagtaat taaatggatc gagtggacaa
    14161 aatgggatcg cggtatttat ctgattgtct ttacggtctt cctcggaatc acgttatatc
    14221 gtgtatttac agagatccaa gtaccagaag gacaaagcat ctgggctcca ggcaattcgc
    14281 tttctgatat ttggatcaat acgaatacaa tcggaagttc tttgatgact cttgctttat
    14341 tgatcagtgg atttgccagt tcttttgaac gatggtatgt tcgaatcatc ggtgggatag
    14401 cagtcgttgc agccttttta ggtatttggg tctgccgatc tagaggtgca ttagtcgcgt
    14461 tgattgtttt tgcgatattg gatcttttac ctaaatcgtt gatgcgtgtc attcgtgcgc
    14521 catttattgc ctacacggtt acgattcttt tagctttgcc gatttcttat ctggcggctg
    14581 tctctgaaaa agtcaatttg tttaccggtc gagaagatat ttggcataaa ttctatcaga
    14641 cgctaggaga aaaatctgaa caagttttac taggaatgaa aacatttatc ttccagcgag
    14701 gaaaccaatt tttaggaaac cacaatagtt ataattctat tttgaatatc tatggattga
    14761 taggatttgg gattgctgct ttgctgctta tcctttttat tggtcgctta acgttgaaag
    14821 cagatttatc caacgggcag atgactttta tctgggcctt ttttgcggtg atgatgcagt
    14881 catttatgga ggatacattg acttcatacc catgggttcc gattatttat atcctgttgg
    14941 ctatggccgt tcatcgatat gatgaaccag aagttacgga ggaattctat gttatggaag
    15001 aggcatatga cgatatgact tatgatgata catcttatga ggaacaacca gatagcgctc
    15061 attcttccag gatccaacgc tatcattgat aggcacaaaa tacgagagag gctgtgacaa
    15121 ccgtcgcagc cttttttggt ttattgcaat tgtctagcat cttctgtaaa atagaaacgt
    15181 tagagaaaat cttgtatcat agaaaattaa gaaagaaata agaacgcagc tcggtaccaa
    15241 aaatatggta aaatggcact tgactgtaat tcactgaaaa tcgcttggag gaacacaaat
    15301 gtttaaagat ttgtatttat ttatgaagga catttatcag aacagaaaat tactgctaca
    15361 gttttcgatc aatgacttca aatcaagata tgcaggatcg tttttaggaa tcttatgggc
    15421 gtttatcaat ccgctattta ctgtattgat ttattggctg gtatttggtg ttggactgaa
    15481 agcacaaatg atcgatgcaa aatacccatt tatcgtatat ttagttaccg ggatgattcc
    15541 ctggttcttc ttctcagatg catttgcatc aactacggtt gtttttcggg aatatactta
    15601 tttagtaaag aaagtcgtat tcaatattcg tatcttgccc acgacgaaaa tcctatccaa
    15661 tctgtacacc catttattct tcattctgat tggcatagct gtcgcgctta taaatggtat
    15721 atatccttca gcaatgtctt tacagctgat ttattattta ttttgcatgt gtgctttctt
    15781 gacaggattg acttggctga cggcatcgat ccaaccattt ttacctgata tcatgcaatt
    15841 aattacgatc gtgctgcagt tgatcatgtg gacattaccg atattatgga gccctaatca
    15901 gtttgattca ggtcttgtaa gcatattgaa attaaatccg ctgtactatg ttgttcaagg
    15961 ttatcgtgag tcatttttaa gtgaagcttg gttctgggag catggaacat atactttgta
    16021 tttctgggtt ttcacgttag taatgttcgt aatcggctct gcagtgttcc gtagattgaa
    16081 accacatttt tcagatgtgc tgtaaggaga atagctatga cagaatatgc aattagacta
    16141 aaagatatca caaaatctta taatatgtac gcaaaacctt cggatcgatt tcgtgaagcc
    16201 ctcaatcctt ttaagaaatc ctatcatgat gtattttatg cgttgaaaaa cgtgaatgta
    16261 gaaataaaaa aaggtgagat gatcggtttc gtgggagaaa acggatcggg aaaatctaca
    16321 atgttgaaaa tcattacggg agtactgaca ccaacatccg gcacgatgga aatcgaagga
    16381 aaaatctcag ctttgctaga actaggatca ggattcaatc ctgagtattc tggctatgaa
    16441 aatatctatt taaatggaat ggttctagga ttttcaagag aagaagtcga tgaaatggtc
    16501 gatgatatca ttgaatttgc tgacatcggc gaccatattt atcaacctgt aaaaacctat
    16561 tcaagtggga tgttcgttcg tctagctttt gctgtagcga tcaatgtgaa tcctgatatt
    16621 ttgattgtgg atgaagcatt ggcagtagga gatcttgaat ttcaactaaa atgtatggaa
    16681 aaatttacag aaatcaagaa ctcgggaaag acgattttat tcgtttctca tgatattagt
    16741 tcgattcgcc gtttttgtga tcgttcctat tggttgaaaa atggaaaagt tgtcgaatat
    16801 ggtgatacga tggaagtaac gacgaattac gaaaactttt tgaagaaaaa atcagttaaa
    16861 acagtagatc gaaataaaaa ccaagtagaa cgcttcgcag ctccagatat cgtagacgtg
    16921 acgaaagccg aattgttgga taaagataaa aatccagtgg atatgctgga acaaggagaa
    16981 aaactatacg ttaaagtaac ctatgaagta aaagacgata caatcaaaaa tccagtttta
    17041 ggaattgctc tgcgtacagt cgacaatttt tatgtgtgcg gactcaatac tctactagat
    17101 ggtatcaaga tcccttggaa aaaaggtgag aatatctttt atctggagta tgaaaagtta
    17161 ggtttgttag ctggtgaata ttattttgac gttgcggtct ttgaagaaaa tgcaacggtt
    17221 cctttggtat acaagacaaa atacatgaac ttgtttgtca gcggttctta tatcggagaa
    17281 ggcatcgtgg tcttagacca taaatgggaa gaagggactc atagcaatga aatatgattt
    17341 tgaaatggac ctagatgaac aaagcagtgt gggaaaaatt gccgcacaga tcaaaccagg
    17401 aagtaaagtt ttagagtttg gtcctggaaa cggccgatta acgaaacatt tgatcggagc
    17461 aaaacaatgt gaagtgagca ttgtcgagct agataaggaa ttgttcgatt ttgtcagcga
    17521 gtttgcccaa gatggatttt atggagatat cgaaagtttt gaatgggcaa actattatgc
    17581 tggacaaaca tttgattacg ttttgtttgc cgatgtttta gaacatttgg tagatcctgg
    17641 aaaaacgttg aagaaagtta gagaattttt gaatgaagaa ggagaaattc taattacatt
    17701 ccctaacctt gcgcataatt ctgtcatgat tgatttgttc aataatcaat tgccatgggc
    17761 ttcttatggt ttgctagatg aaacccataa ttctttttat actcatgatg gcttccaaaa
    17821 agtttttgaa aaagcaggat tatacatcaa tattgaagat tatttgtact tagctgtagg
    17881 tgatacagaa ttaaaatcaa cttatgaaga attgccggaa gctgtacgtt atgatttcaa
    17941 aatgcgacca tttggcgaag tctaccaata cttcttctca ttgatgaaac atccagtagc
    18001 acaaagttcc attgcgcagc cgcaaaattc taattatgtg aaagtattag aagtaatcca
    18061 gcaaacgaaa caagatgaaa taattcaaca aataccattc aataatttca caggggaaaa
    18121 tgagacacta agtttccatg tatcagaatt agcagaacga atggtgtttc gcttcgcgca
    18181 gcaacctagc tttatcgaat tcagcgcaga agctgctgga gaaaaactca catttatcga
    18241 ttcaaatgct gtagtcaaaa cagtaaatga ttgttatttg tttgatggta aagatttacc
    18301 agagttcttt ttaacggata ttgcagggaa agaagtgact atccattgtc actatcgttt
    18361 tatcggagaa ttgacaccaa caatgaaaga attgctggaa acaatccgtc caatggctga
    18421 agttacaaag cagctttctg agaaaaatga tgaattagaa agagaaaatc attatcttat
    18481 tgagaataat acaagattag atcagacgtt gaaaacaacc ataaatcgct attgcacgtt
    18541 gttggaaagt gatgaatgga caatcaaaca tagattaggg cgacgtaaga aagaaacatc
    18601 gaaaaaaatc caagaaaaag agctttctct atgtattgac gaaaaaatct gggatgcaga
    18661 aacaaagata ttgaaaatca tcgggtgggg aatcgctaat tcggatcgcc aaccattgtc
    18721 ctataagtta tcagccgacc aatcaccatt ttttgaagca attcctgttt tacgagaaga
    18781 agtcaatcaa gcaaaacagc tagctgaagg agcaaaagca ggatttgaac tgcggatcct
    18841 ttgcgagaag gaacgttcat tcttagttga agcagtggct caaaatggtc aatcatggat
    18901 cattgaaatg taaataatga ttaacttata aagataagga gtaaacgaat cgtgcaaaaa
    18961 aaagaaattg ccgtatttat cgatcaaatc actcgtgaac gggccactgg cgatttgctt
    19021 attgtgggct gggcaattga tgaagtgacc aaagaaatcc ctacgataaa ggtagaaaaa
    19081 gaaaatgtga ttgctgaagc aacacatgtc gtaagattgg atatcaatca tttatataat
    19141 cttgatgtta agacccaaag cggttttaag gtacgtttat cagggaaaat gagaggaaaa
    19201 gcaatcttag acttccaaac agcaagacat caaaatggca tagctgtgaa attaaacggt
    19261 aaatatcctt atgatgacgg cattgaatct tcatgggaaa gaaaaaaaag attactgaaa
    19321 aaagggataa attatgcaag gactcatggc gtaaaaaaag caatccgccg ggtcaaactg
    19381 gaattgaaac caggatcgat cgactatgca gagtggatca gtcgtcatga gaaacctgat
    19441 ctgaaagagc aaaggaaaga agtacaagga tttgcctacc gcccattgat ttccatactt
    19501 atgcctgtct ataacgttga aatcaaatgg ctcgaaaaat gcattgattc ggtacttgat
    19561 caaacgtatg atcactggga actatgtatc agtgatgatg cttctactga tccagctatc
    19621 cgaaagtgtc tagaatccta tcaagcaaaa gatgatagaa ttaaagtcgt tttccgacaa
    19681 gaaaatggac acatcagcct ggcaactaat tctgcattgg aaatggcaga aggagagttt
    19741 atagcgctct tagataatga tgacgaatta cctccatttg ccttatatga agtggcaaaa
    19801 gtgctaaacg tacatccaga actggatttg atctacagtg acgaagataa aatcgatgca
    19861 gatgggaatc gctttgaccc gcacttcaaa gcagactggt ctcccgatac attgatggga
    19921 aataattata tctctcactt aggcgtatat cggacaagca ttgttaagga acttggcggt
    19981 ttccgtaaag gatatgaagg ctctcaagat tatgatttgg ttcttcgtgt gacagaacaa
    20041 attcctgcag aacacattta ccacattgac cgtgtcttgt atcattggcg gacgatcccg
    20101 ggatcaactg caagcaatgg agaagctaaa agctacatct atgattctgg agtgaaagca
    20161 ttgacagatg ctctatctag aagaaatatc aagggaagcg tacatcctgg cagaatttct
    20221 ggtttttatg aaatcactta tgatgttttg caagaagact tggtcagtgt gatcattcca
    20281 actaagaatg gctatgaaga tctaaaaact tgtgtggatt cgatcatcga aaaaacgtct
    20341 tatccaaatt atgaaatcat tattgcagat aatggaagta cagatcctaa aatgcaagaa
    20401 ttatttgcag aatacaagca tcagctgaaa gaccgattta tcgtcgaatt aattgacatt
    20461 ccattcaatt attcacgtat caacaatctg gcagcagaaa aagcaaacgg caaatacttt
    20521 ttattcttga ataacgatac agaagtgatt gaacctgact ggatgacagc gatggtttct
    20581 tatgcacagt ttgatcgaat cggctgtgta ggagcaaaac tgttttatcc agatgataca
    20641 acgcagcatg caggagtatt attaggaatc ggtggtgttg cagggcatgc attgaataac
    20701 tatgatcgaa cacattgtgg ttatttcggc cgattggtca ttgacgtcaa ttacttagca
    20761 gtcactgcag cttgtatgat ggtcaaggca gctgacttca atgctgttaa cgggtttgat
    20821 gaaacattag aagtagcatt caacgatgtt gacttgtgct tgaaagttta tgaactgggt
    20881 cgttacaatg tctatgcaca tcaagcagaa ttgtaccatt tcgaatctaa atcaagaggt
    20941 tatgaagata ctccagaaaa acaaagacgt tttgctggtg aaatcaaaaa aatgcaagat
    21001 aaatggccag catatattgc gcatgatcca ttttacaatg ataatttaac aaaagaagga
    21061 atcggcgact tttcgttgag accagattaa actataataa aaagctgtta tgttgtgatt
    21121 cgctcgcaca taacggcttt ttatagtttt ttgtcaaaaa aaggcagaag gtgccgactc
    21181 aaaaaaataa gtcgaggttg ggacagaagt gtttaactcc gagaaataag aagaaattca
    21241 cgaaaattgc ttttcaaatt tttgtgaatt tcggcttatt taagaagaag ttgcttctgc
    21301 tcccaccgtt tattcgtttt taaagaatga gacaaaagtg tttcttactt ttgtcccacg
    21361 ctcttattca tgctcagagc gccaatagat acaaaatcat agccaaagtt tcacgaaccc
    21421 tagctgtcaa agaatcgata ttttatttta atataggcta ttttaaaata aatggtttaa
    21481 ggtattcctc tacaaaagcc actctatcaa ttatcatggg tttaaaataa gaagtgatgg
    21541 agataaccag attgttctta gcgtcaatat aaagaacatt tccactatta ccaattgctg
    21601 aatatatttc gttctcagaa tctaataccc accataaata gccatatctc atattttgta
    21661 agtcttctaa agcactggtt ctaggtttga acatctcgtt tatccacatg ggtggtacga
    21721 tttgttttcc tttatacatc cctttattta ggcataatac accaattttt gccagatcac
    21781 ttgccgataa acacaaacca tatcctgccg tcccgatatt tagtggatca caaaaccaga
    21841 cattggtttt aggagactta tccattgtaa actgcttatg ttcttcagct gtttcagcat
    21901 aataattgac atggtttggt atccctatag gatcaaatag atacttattg gcaaaatcaa
    21961 cagttgttag cttacttgtc tgatacaaca agccagttaa tatgtgtaaa cagacagttt
    22021 gatagttgaa ttcgccggtt ataccttttc ttcctcccaa taaatctagt gatgtttttg
    22081 tccaatcttc acttgaacag acttttgtcc atggatcgcc tttacatttg tagggtgctt
    22141 tcattgttag caagtgcttg attgtaatag cgtaaatagt tttctccccg cgtttcactt
    22201 tatactctgg aaaataatct aatactttat catcgatact tttgattaat ccttgctcta
    22261 aagcaatgcc aatcagcaaa gatacaatac ttttcgttgc tgacatgata tgacaagtat
    22321 cgtctttttt ataaccattc cattcatctg tataaacttc taccccatcc ttatatgcga
    22381 caatttgaca tatatttggt tgtcgaaaac caataaaatc atgcaactct tgtttattca
    22441 taagatatta cctcactttc ttctataagc ataatgtcat taaaaatcga tttcaatttt
    22501 ttcgattctt cacgtaatac aggtattctt atggactaga cagcgctttg ggaagatgga
    22561 ttctctgaaa aatataaagg ttcactttat gagtggataa aaaaagcagc aaatacttcg
    22621 acttctttaa tttaccaaca ttagtgaaaa tcgattcatc tggagatttg taatttagag
    22681 tccttgtctt gattagaggt gtataaagct ttttattaga catttttaaa ttccattaca
    22741 atttgcttat aaactaggtt tttaaaggtg actatggtaa gataatagag atattatttt
    22801 gtgattagga gtgtatatat gaataaaaat ggagaatgga gtgctgcacg acggatattc
    22861 attatattaa tggatgttat tgtatacaat gcagcaatat atagtagttt cttactgaag
    22921 tttcgtggag agatcccatt acgaaattta gaaactttcc aacactcagc aattattatt
    22981 tcagtgattt ttattgcgtt gaacattttg ttgggtgcat atgtttttta taatcgaatg
    23041 atcagtgaca tcgtgtttgt cacagtcatc ggtcaggtat tgatgacact agggatcatg
    23101 gtagtgactt ttgcgggccg ctggtttgct tttccacgtc ttgttatctt actttcattt
    23161 gttctcggta cgcttttctt aagtatctat cgtattctcg tatataaact ttatttacgc
    23221 gtaagtcgag ataaaaaggt cgtcattatt gggttagaaa aggatgtcgc accagcaatc
    23281 caaaattttg gttcaaagaa aaacaacaaa cataaagtcg aagcagtcat tcttgataac
    23341 tattatgcaa acatcaaaaa tatgattaaa gacatcgata ttgtctatct tgcttctcat
    23401 atcgaagaaa ctgaaaaaat caaaatctat caattagtta ctaaaaatga aaaaaaattg
    23461 tttttgaata caacatttga aaacttgaca ttgatcaatc caaatattat gaatttcgaa
    23521 gatgaaagta ttattgaagc atctggattt agaatccaac ctgaatatga actattcaaa
    23581 cgtttgtttg atatcatcgt ttcacttgtt ttattagtta ttgcttcacc atttatgtta
    23641 ttcacagcaa ttttagtcaa agtgacatca ccgggaccaa tcatctataa acaagtgcgg
    23701 atcacaaaaa atcaaaagga attttcaatc tataaattcc gctcgatgac cgccacagcc
    23761 gaagcaaaat ctggaccagt tttagctaag agcaatgatg cacgagtaac gcctgtaggt
    23821 aaatttattc gagctgtccg ctttgatgaa ttaccacaaa tcttcaatgt gctgaaaggc
    23881 gatatgtcga tcgtaggacc acgtcctgaa cgtcctttct tcgtcgatca gttcaatgca
    23941 gaaaaccctt attactactt acggcataat gttcgtgctg gtatcactgg gtatgcacaa
    24001 gtctatggaa aatatgtctc tgattacaac agtaaattac gttttgattt actttatatc
    24061 aaaaaatatt cattgatgct tgatttaaag atcttgctgc aaaccatcaa gatcctattt
    24121 gataaaatgt cctctcaagg acttgaagaa gacgaaaatg tgatctcatt ggcagaaatt
    24181 gcttttccga atgaaaaaat ctttcgctag ttgggaggcg taaaaatgaa caatccgaaa
    24241 ttcagcataa tcgtaccagt atacaatgca gaagcaacta ttggtcgaac gattgatatt
    24301 ctaaaagaat tggattatga agagtacgaa gtcgttttag taaatgatgg atcaacagat
    24361 caaacacagg aaatcattca agaactgatt gaaaatgatg aacgttttga attgattact
    24421 acgaccaatc aagggcccgg attagcaaga aacgaaggaa taagacagtc aaaaggagaa
    24481 tatctcttat tcttcgatgc agatgacacg ccagtaaaaa cgatcttgtc agattatgat
    24541 catttgctag aaaagagtcc aaaagcggat cttgttgtta gttcgtttgt ttttcgtaca
    24601 atggacgagg gaagaatcgt ttccgaaaaa gaaaacttag ttacagaatg tcactatcta
    24661 actcacgaag agtttataaa agacatgtat cagttaatga atgaacagtt gatgtatgtt
    24721 gtatggaaca aatgctatcg tagcgatatt ctgaaaaata accaaattct atttaaaact
    24781 tataatagtt gtgaggatcg aatatttaac ttacattatt atgaatactg tcatcaagtg
    24841 atcatgaacc caaaagtaga atatatttat gaatttgaag gtgggaaggg gatcactaat
    24901 caataccgac ctaacaagtt cgaaacattt aaagaattct attcattagc aaatactgta
    24961 acagacgata ttaataaaga cggtatggcg gcgcttcttt taaaagggac aacttcagtt
    25021 attttttcta tttatagtac ctctgcaaga acttcaaaag agaagaaaga cgaagcaaag
    25081 cagatattga gtgatccagt gatccgagaa gccaagcaaa ttgctaagac ggattcttca
    25141 gcaaaaaaaa taacaaagtt gctttacaat atgccagctc cactattttt atctgctgta
    25201 aaagttggaa gcttcgttga agtgaaaatg ccagggttaa tggctgcttt gaagcgagta
    25261 tattagttaa tggaggaaaa tataatgaaa agaattttgc attttcaggg acgaatgggt
    25321 ttaggtggag cagagtcttt catgatgaat ttatatcgta aaattgatcg tacaaactat
    25381 caatttgatt ttttgattta tgaagattat gcagatgttc aagactatca ttcagagatt
    25441 gaaagattag gaggccgtat ttttgttgta cctaatccta agaaaaatat ttttaaatat
    25501 ttaatagaag tgaataagtt actaaaaaaa gaatcttttt ctatagttca caatcaggtt
    25561 tattttggcg ggggaattaa tttatggtta gccaaaaaga atggaatcag acagagaatt
    25621 gctcatagcc atgcaactga agatggaaaa tctcaaaata ttgtaatgaa tgtattgcgt
    25681 aaatttttaa ccaaattgtt gttacaaaat gcaacagact accttgccgt tagtcaagaa
    25741 gct
//
LOCUS       DACDIE010000037        25289 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.037, whole genome shotgun sequence.
ACCESSION   DACDIE010000037 DACDIE010000000
VERSION     DACDIE010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25289
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.037"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..3107)
                     /locus_tag="IV250_001678"
     CDS             complement(<1..3107)
                     /locus_tag="IV250_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAP8768965.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWSNLRQ
                     KIISNNQAELNDSPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIERENPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIIQIELK
                     QVTAKDGFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGVSSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMESVKKESYLDPTVFEAEEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKESDWLIKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTAWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVQSSTLNNQIQLLEE
                     QIGVYETVKGSLMEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDNLIHA
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRK"
     gene            complement(3121..4338)
                     /locus_tag="IV250_001679"
     CDS             complement(3121..4338)
                     /locus_tag="IV250_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP8768966.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLGDMMDVTSVKRIHQSDWTNSG
                     IRFLRARDIVSAAKNEEPSDYLYISEEKYNEYSKNSGKVSQGDLLVTGVGSIGVPLLI
                     TDDNPIYFKDGNIIWFKNEHKIDGNFFYYSFINNKIQKYIRDVAGIGTVGTYTIDSGK
                     KTPISLPTYDEQIKIGSFFKQLDHTIMLHQHELDLLKETKKGFLQKMFPKNGAKVPEV
                     RFPGFTGDWEERKLGEVVVFLNGRAFKQQELLDSGKYRVLRVGNFNTNDRWYYSNLEL
                     EEDKYANKGDLLYLWATSFGPEMWNEERVIYHYHIWKLKFLSKELDKQYLYTWLLTDK
                     EKIKESTNGTTMVHVTKGGIEQRTFQFPPDINEQQKIGAFFKQLDDTIALHQRKLDLL
                     KETKKGFLQKMFV"
     gene            complement(4328..5923)
                     /locus_tag="IV250_001680"
     CDS             complement(4328..5923)
                     /locus_tag="IV250_001680"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAP8768967.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYSISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHEQ"
     gene            6048..6833
                     /locus_tag="IV250_001681"
     CDS             6048..6833
                     /locus_tag="IV250_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family restriction endonuclease"
                     /protein_id="HAP8768968.1"
                     /translation="MPNKNRTFESILNEGLTTEPASIIFDEVIDDVEDSKYHKNLKEL
                     FYFALNNGPSLFTGISVPDSDNPLIMAKIYLTKWCNKYIRDRNNPALKKPLKTFGEKD
                     AALTTRVAANANIDDQKVLNDYLRGHFLYMSAENMNGSILEEYLAEVLEPEGWIWCAG
                     SVYRAVDFCYLGTSPILLQVKNKYNTESSSSSAIRVGTTIRKWNRLNKSTKISGLDSP
                     IPNWKALIEMTEASKELAAKLTENSYLAYINEKSTRELWTLDD"
     gene            7542..7901
                     /locus_tag="IV250_001682"
                     /pseudo
     CDS             7542..7901
                     /locus_tag="IV250_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(8196..9329)
                     /locus_tag="IV250_001683"
     CDS             complement(8196..9329)
                     /locus_tag="IV250_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP8768969.1"
                     /translation="MLLNFKMTLTMLAFSLLVTGVILVSFFFLRNNLEGISIASTLGE
                     DVYFMEEGKTVQAETQMEASKSSNNQFMTISMIDKAYQDELFKYLPAMVLVICSAVML
                     LTFILWLVLRKIYSNQILAITRDLTFIDQVPKNTVKDEALNAAFNKLKDKFDGHLEDY
                     RRLNSYLTHEQKNAIAILRTNLEIERQHADQGNLMLLDRMTDSIDDILTLSNNSEDLS
                     TEVVDVSLICAEVCDAYKMNYPNLLFAFTEDQPTLIHGRDRWIYRAVSNLVDNAIKYG
                     EGKLVIVTISNQKGSVIIEVKDQGIGIPQEKQASIFSHQYRVHGLKQDGLPKTMDFRD
                     TDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDILSSLI"
     gene            complement(9320..9988)
                     /locus_tag="IV250_001684"
     CDS             complement(9320..9988)
                     /locus_tag="IV250_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8768970.1"
                     /translation="MRVLVVEDNRELVLSVKKGLEQENISVDVAYDGESGEEKAFINS
                     YDGILLDLNLPKKDGLSVLQSLRAEEVESPVIIITARDEIEERAKGLDFGADDYLVKP
                     FELLELKARIRAVIRRYHGRALPQISIGALKIDPLGRRVSYQKTEVVLKPKEFDILLC
                     IAQRHPAVVSSEEISEHVYDEDFDPFSSVLRVHLARLKKKLAEAAEKEVLKTIRAKGY
                     QLCY"
     gene            complement(10444..11430)
                     /gene="lacD"
                     /locus_tag="IV250_001685"
     CDS             complement(10444..11430)
                     /gene="lacD"
                     /locus_tag="IV250_001685"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP8768971.1"
                     /translation="MNEEKRQYIKQLCNEDGVIGALAIDQRGALKKMITKFKGSEAEE
                     SEIVEFKEIVSSQLTKYASSILLDPEYGLPAADVRAKNAGLLLAYEKTGYDASTPGRL
                     PDLLSIWSVKRLKEAGADACKFLLYYDVDEAEEINNQKHAFIERIGSECQAENLPFFL
                     ELVSYDANISDATSKEYAIKKPHKVNDMMKEFSDPRYGVDVLKVEVPVNMNYVEGYGT
                     DEVVYTKAQAANFFKEQAEATNLPFIFLSAGVKAELFQETLRFAKNAGSTFNGVLCGR
                     ATWADGVESYIVDGKEAAMEWMNTQGRKNIEELNAVLKETATPVEVLTSSLS"
     gene            complement(11443..12369)
                     /gene="lacC"
                     /locus_tag="IV250_001686"
     CDS             complement(11443..12369)
                     /gene="lacC"
                     /locus_tag="IV250_001686"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HAP8768972.1"
                     /translation="MILTITMNPSIDISYPLETFKLDTVNRVRNVRKTAGGKGLNVTR
                     ILKQLDTEVIASGLIGGFLGEEIKKDLTQNEIAHDFLPISGETRNCIAVLHDGKQTEI
                     LENGPVITEQEARAFLQHFEKLAVKAEIISFSGSLPEGLPADFYRQMLQVCQPKPVVL
                     DCSGTALREVLRGSVKPKLIKPNTEELAGLLGREISTELSDLKAALSDSMFDEIEWIV
                     VSMGGDGAFAKHHETFYCVTIPKIDVVNPVGSGDATVAGLTAAIQQGLSDEAVLKQGN
                     VLGMLNAQEATTGYVQLANYQKLYDQILVEKV"
     gene            complement(12382..12897)
                     /gene="lacB"
                     /locus_tag="IV250_001687"
     CDS             complement(12382..12897)
                     /gene="lacB"
                     /locus_tag="IV250_001687"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HAP8768973.1"
                     /translation="MKIAIGCDHIVTDTKMAVSDFLKSKGHEVLDMGTYDFTRTHYPI
                     YGKKVGEAVTSGEADLGVCLCGTGVGINNAVNKVPGIRSALVRDMTTAIYAKEQLNAN
                     VIGFGARITGEFLICDILEAFINADYQETEENKKLIAKIDHVEKASDKQSDPHFFDEF
                     IEKWDRGEYHD"
     gene            complement(12916..13344)
                     /gene="lacA"
                     /locus_tag="IV250_001688"
     CDS             complement(12916..13344)
                     /gene="lacA"
                     /locus_tag="IV250_001688"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HAP8768974.1"
                     /translation="MRVVIGSDSDGMELKELIKKKLIKEGITVVDKSETASEDFVEST
                     LAIANDLNENEGSLGIAVDGYGAGSFITATKVKGMVAAEVSDERSAYMTRKHNNSRMI
                     TLGSKIVGDELALNIVKEFLQANYDGGRHQVRVDMLNKMA"
     gene            complement(13364..14137)
                     /locus_tag="IV250_001689"
     CDS             complement(13364..14137)
                     /locus_tag="IV250_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP8768975.1"
                     /translation="MLKTERQRSILAMCDRYGTITVKFIQEVLNVSDMTIRRDLEELA
                     QKKKLLRVHGGAQKIDYLEMSTSIEANELSHTEKKKLHTKEKEYIAKNAAAHINQEDD
                     TIFLGSGTTIELMTRYIKPSSLRIVTNSLPVFNLLSEIKRFELYLIGGLYREKTGAFV
                     GSIANEAVQKIGIKKAFVGVNGLKGSEVSNFSIEEGTLQKIVLDNAAEKCLVSDANKF
                     DHSDFYNFYDLRKIDKLFTDSTLSAELKSYYQQFTKIIQ"
     gene            14302..14781
                     /locus_tag="IV250_001690"
     CDS             14302..14781
                     /locus_tag="IV250_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8768976.1"
                     /translation="MNEFFFKDTVYVSDKQSQEEVFEEVYQDLLKKELVTEDFLENLL
                     ERERNYPTGLDLSPVSKILPDIAIPHTESEFVRTTRIIPIKVKNEIPFYNMISPSDQI
                     SASFLFMILNENGEAQAGMLANIMDFINSVDEEKLFSFFKLEDTEAIYHFLEEHFYI"
     gene            14838..15140
                     /locus_tag="IV250_001691"
     CDS             14838..15140
                     /locus_tag="IV250_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003088862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HAP8768977.1"
                     /translation="MIKILAACGAGVNSSHQIKSALETELKNRGYQVTCDAVMIKDIN
                     EEMLSHYDIFAQIANTDLGFDINIPVVDAGAILYRIPAMAEPVYQSMESAIQSLNK"
     gene            15162..16619
                     /locus_tag="IV250_001692"
     CDS             15162..16619
                     /locus_tag="IV250_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol transporter subunit IIA"
                     /protein_id="HAP8768978.1"
                     /translation="MNTIIDIANKVFTPLINLGAAPLMTIVLTVIALFFKVKPSRALE
                     GGLKLGIALTGIGAIIGILTTAFSDAMTAFVERTGISLNITDVGWAPLATITWGSPYT
                     LYFLLIMVIVNIVMLAMKKTNTLDVDIFDIWHLAIVGLFAIFCGANLFIASALVIFIG
                     VLKIWNSDLMKPTFNDLLNAPDSNPMTTTHMNYMMNPIIMVFDKIFDKIFPWLDKYDF
                     DAAKLNSKIGFWGSKFAIGIYLGIFVGLLAGQTPTQIFSLAFTAAVCLELFSLIGAWF
                     IAAVEPLSQGITDFANKRLKGRTINIGLDWPFIAGRAEIWAAANVLAPIMLLEAIILP
                     GNKLLPLGGIIAMGVTPALLVVTRGKLIRMIVIGAIELPLFLWSGTLIAPFVTQTAKA
                     VGAFPAGLNGSAMISHTTMEGPIEKFLGYLVGNASQGQIEFVIYAALALGAYLLIFIW
                     YARQMKKRNAAYAAEKEENASPATAHNMTYAAEAK"
     gene            complement(17212..17964)
                     /locus_tag="IV250_001693"
     CDS             complement(17212..17964)
                     /locus_tag="IV250_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP8768979.1"
                     /translation="MLKTDRFQAIMSLLEVNGTVRVSDIMEKFSVSDMTVRRDLDEME
                     KQGLLERVHGGAKLKDFYRHVELSHREKQIMLQEEKQRIVEKANEMINDGDTIFLGPG
                     TTIELMSKIIQGDSLRIVTNCLPVFEELNKRKGNRKIYLLGGEMRETTQCFVGEITNK
                     SLQDMNFHKAFFSCNALNEQKIMTSTFEEGQTQQIALNNSVERFLLIDHSKIGKRDFS
                     VYYNLNDVTVVITDDDEQKHYEKIEKEEKYVV"
     gene            complement(18179..18415)
                     /locus_tag="IV250_001694"
                     /pseudo
     CDS             complement(18179..18415)
                     /locus_tag="IV250_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
     gene            complement(18425..19389)
                     /locus_tag="IV250_001695"
                     /pseudo
     CDS             complement(18425..19389)
                     /locus_tag="IV250_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(19595..21277)
                     /locus_tag="IV250_001696"
     CDS             complement(19595..21277)
                     /locus_tag="IV250_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8768980.1"
                     /translation="MNTIIKQIEKGKPFFEKISRNIYLGAIRDGFLTAMPAILFSSIF
                     IMIAAVPEVFNVTLPEAFSTWLWKVYGYSMGLVGLLVVATTARCLANSMNRQLSDSGK
                     QINEVSVMLASICGFVMMAADQIDGGIATDYLGTKGLLAAFVSAFVTVNVYKFCVKRD
                     LTIRMPKEVPGTITQMFRDIIPFSIAVFIAVLIDTGVRQFFGTSFAAGVIQLLQPLFS
                     AADGYVGIAIIWGAMAMFWFVGVHGPSIVEPAIAAIIYANVETNFELFKNGAQASKVL
                     TVGLGNFVGTMGGTGATLVVPFLFMLFAKSKQLKAVGKASFIPVSFAVNEPLLFATPI
                     ILNPYFLIPFLLAPIANVWIFKLFVDVLGMNSFISVLPWATPAPIGLVLGTGFSVLAI
                     VLAVVLIVVDAIIYLPFVKAYDSELVEQELKNIAAEEVKEELVEEIQEVGATAAVIVD
                     EAKASVGEGKRVLVLCAGAGTSAMLANALEEGAKETGVNLSAKAGAYGSHYDMMKNFD
                     LIVLAPQVNSYLDDLRKDADKLGIKVIATKGAEYINLTRDPKGAVDFVLKQF"
     gene            complement(21288..21611)
                     /locus_tag="IV250_001697"
     CDS             complement(21288..21611)
                     /locus_tag="IV250_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP8768981.1"
                     /translation="MDRKELQLLGFEIVAYAGDARSSLLTVLKEVREGNFDNVERLLA
                     DADENLTLAHNSQTKCLAEEASGNEMEVGVIFIHGQDHLMTTILLRELINDFVELYKE
                     KFAKA"
     gene            complement(21634..23040)
                     /gene="lacG"
                     /locus_tag="IV250_001698"
     CDS             complement(21634..23040)
                     /gene="lacG"
                     /locus_tag="IV250_001698"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAP8768982.1"
                     /translation="MNYKTLPEDFIMGGATAAYQAEGSTKEGGKGQVAWDTFLVEKGR
                     YTADPASDFYNQYPVDAELCKKFKIDSIRMSIAWSRIFPKGYGEVNQTGVEYYHKVFA
                     ELNKHGIIPFVTLHHFDTPKELFDNGDFLNRENVEHFVNYAQFCFDEFSEVHYWSTFN
                     EIYPVASNQYLIGSFPPEVQFDIEKTVTCLHNMMYAHAKTVNIFKDGGYKGEIGVVHS
                     LETKYPASDKEEDKHAAKLDDALSIKFLLDATYLGYYSDETMAHINEILAANGQSADI
                     DSNDFREMKKAANRNDFLGINNYGSHWVKEYKGESTLHYNTTGEKGSQVYCIKGIGER
                     IVREDLPKTDWDWIIYPKGLYDLLVRIQDEYPNYNKIYITENGLGYKDNFEDGIILDD
                     DRIDYLRQHFNAISDAIKMGVNVKGYFIWSLMDVFSWVNGYTKRYGLFYVDFDTQKRY
                     PKESAYWYKFVSETKTII"
     gene            23507..24514
                     /locus_tag="IV250_001699"
     CDS             23507..24514
                     /locus_tag="IV250_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8768983.1"
                     /translation="MVTIRDIAKKSGYSTATISRLFKGDESLSITSDTKNKIIVTAKS
                     LGYDLTKIKTTLYKIAILFWISEEQLKKDVYYQHVKDDLKNYSKKANLDLIFISKNDG
                     VKSIPDDITGFIAIGQLTKKELYYLNQKGYKGVIRGINPLPEHFDTVEPDFHEMARQA
                     INYFINEGFTKIGFIGGNFFNLETGKEEIDSREYAFRYHLEKSGLLNEKYIFSQGSFT
                     VDEGYQLTLKMKAELNEDMPEACFIPSDTIAAGVLQGCHELGINIPKDMAVISINDDD
                     IAKFVAPPLTTFKIDIEEIAKTCVDLLSDQLVYSRDISKKTLIGAKLTVRKSFIPKEE
                     V"
     gene            complement(24908..>25289)
                     /locus_tag="IV250_001700"
     CDS             complement(24908..>25289)
                     /locus_tag="IV250_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-dehydro-3-deoxyphosphogluconate aldolase"
                     /protein_id="HAP8768984.1"
                     /translation="NSLRYLEFREGAFDFQTSQQNLLTIPGAMSPTEIFKMFEMGADI
                     VKVFPANCLGPEYFNQVQAPLGSLPLMAVGGVDQTNAQNYLNNGASYVGIGSKFFEKS
                     AVHQLNYERLMELAESFIDSLRVE"
BASE COUNT         7531 a         5269 c         4188 g         8301 t
ORIGIN      
        1 attttacgcc atgctcgctt catttcttgt gggtatttaa atggtttcgc ttctggatgt
       61 ttggctttat atccttcgta atccttactg ttgataattg ctttcatatt aggaattgga
      121 tcttcacctg gcatatattg cagattagaa gcatgtaatt ctttctcaga aacaaaccac
      181 tcattggaaa aggcatgaat caaattatca cttgcttgtg taaaatagcg acgtttaatg
      241 acaaagatat cttcattact atctaaagtc cccacatcaa atgcatttaa aatttgtcgg
      301 taaacttctt tgactccttc tgttttattc aatttagcca aagctttttc aatttcttct
      361 cgtgcatctg aactattcgc agcataagtt cctagtaatt gatcaatata agtagaatca
      421 atatcataca acttagtggt attttcacta taaaattcga tcgttgaaaa atcttctccc
      481 tctttttctg gttcttcctc catcaatgaa cccttcacgg tctcgtacac gccaatttgt
      541 tcttccaaaa gttgaatttg attgttcaaa gttgaagatt gcaccatgtc atcattgtaa
      601 tcatcataag tcactaacga ttcatacgca cgattcaatt cttgaaatgc tttgacgtac
      661 tcgatacgga tttcaagtgg cgtatgttca tcaatggatt ccatggatga aacctctttt
      721 aacttaccat atgcttgctt gaatcttttt ttggattctt ggtaacttgg ataaatcaaa
      781 cccgattctt ctttagcttc tgaatacagt tttgtcgcat cctcgacatt cttctccatt
      841 gtcgctggct ttcggaaagt aacaattagt ccttttgttt tttcaggata cgtacggttc
      901 gtacgtgaaa aggcttggat caatcctgca tactctagat tacgatccac aaatagggtt
      961 tgaatcgtag gggcgtcaaa acctgtcaat agacgatcca ctacgattac taaatcgatt
     1021 tgtttcccaa attgtttgaa ttccgctctt tttcgtgcca aacgattatt gatgtctccg
     1081 ttataacgct caatgtctgc tagtgaccag gcagtatcat aatactgatt atattcctca
     1141 ataatttttt gcatttcttc ttgttgggca cttgaatctc ggctattttc ttctaatgaa
     1201 taggtaatag cgattcgtgg gaaatcagga tcctccatcg ttcgtccttc acggataggc
     1261 tgattgggaa actcttttat taaccaatca gattctttcg tcatctcttt gatggcccga
     1321 taatactttt tggccatatc aatggaactc gtggttaaga tcgcagactt cgttggacgg
     1381 ccatttcgaa aatcaaattt tgtataggca ttgtctggac ggaacatttt atgaatgact
     1441 ttttggatat gttcctctgc ttcaaaaaca gtagggtcca aataactttc ttttttgaca
     1501 gactccattt gatcgatcat acgattgatc tgttccggtg aatactctgc atatgtttct
     1561 acttctttta atttccgata gattttattc tctaatgaag tcggctccac ggtgttttca
     1621 tgctctactt gaaatcctaa aaccgagcca tcttctaatg cattcttgat ggtataggtg
     1681 tgcaagacct ctccatactg atcatgggtc gtacgtgcca attgcccttt ggcttgtttc
     1741 cgattctcag ggaaaatcgg tgtacctgta aagccaaacc aagtggattt cggaaacatt
     1801 tttttgattt cttccatatt ttcagcactt aacgctcgat gacactcatc aactacaaac
     1861 acgatatgtt gccccattaa tttttggaag cgattggttc cttttttctc ttcctgtttc
     1921 tttgcatatt tcaacgccgc atctagtttt tgacgggttg taatgatcac aacattcgca
     1981 ttggcatctg ccaacagagt ttcacttaac tctttggcac ttcctgttcc cacgatcaag
     2041 ctattcgctt tggcattacc agaagaaacc ccagtattaa actccgaagc aaatttcgta
     2101 aactctgtgg tggtctgatt atctaaatct ttgcggtcca ctaacatgat cgttcgatcg
     2161 atccccgatt ttcgtgctaa aagcttagta gatacaaaac tagtcaacgt ctttcctgaa
     2221 ccagtcgcat gccacacata ccctgattga tgtttatttg cggcaataaa caaggcttga
     2281 atcgcatgca cttgataagg atgtaacacc atgagggttt tattgtcttg atcttcactg
     2341 acaatcgtat aatccgcaat caagcgatgg gcatctggga tatttagcac ttgtttgcaa
     2401 aactcatata agttttctac ctttttatta tctttcgttc gccaactaaa taaaaactta
     2461 ggatgcaaat ccttcggtaa ggcatttgca aaataccgag tcgtttgttc attggaaacc
     2521 acaaacaact gtaaggtcga aaagatattg ttccgaaaca tgccttcttc agcatatttt
     2581 ttgatttgat taaacgcctg ataaaaacca tcttttgcag ttacttgttt tagctcgatt
     2641 tggataatcg gtaagccatt gatcaataag gtgacgtcaa aacgtcgatc ccgcgcttct
     2701 atgttacttc cacgttttgc aatttgatgg accacttcat aagtagagat tcctccacca
     2761 atgtcttgat tggaataaag gataagtgag acagaaccca attgcggatt ttcccgttca
     2821 atcgtgatcc gagccatccc attttcacct tttaaccatt tcgcagcatc aaatggggtt
     2881 ttcgtccgta gcaataattc ggttttgatc gtttcaaact ctttatcggt caaaggagaa
     2941 tcattcagtt ctgcttgatt atttgagatg attttttgac gtaaattgct ccaaagatct
     3001 tcttctgatt ttagatccgg acgatacgtc cactgattat gtccttcccc gaggacacga
     3061 atcaactgtt cttcgacttc cgcttcctca cggtgactaa ttttcattct tcttccctcc
     3121 ctaaacaaac atcttttgca agaagccttt tttcgtttcc ttcagcaaat ctaacttacg
     3181 ttgatgaaga gcgatcgtat catctagctg cttgaagaag gcaccaattt tttgttgttc
     3241 atttatatcc ggtggaaact gaaatgtcct ttgttctata ccacctttag tcacatgtac
     3301 cattgtcgtt ccgttagttg attctttaat tttctcttta tcagtcaata gccaagtata
     3361 tagatactgt ttgtccaatt ccttagataa aaatttaagt ttccaaatat gataatggta
     3421 gattacacgt tcttcattcc acatttcagg accgaagctt gttgcccata aatataagag
     3481 atctccttta ttagcatact tgtcttcctc taattctaag tttgaataat accatctatc
     3541 attagtatta aaattaccta ctcttaaaac cctatatttt ccagaatcaa gtaattcttg
     3601 ttgtttaaaa gctctaccat tcaaaaaaac taccacttcc cccaacttcc gctcttccca
     3661 atctcccgta aatcctggaa aacgaacttc tggcactttt gctccgtttt tagggaacat
     3721 tttttgtaag aatccttttt tcgtttcctt cagcaaatct aattcgtgct ggtgaagcat
     3781 gatggtgtga tcaagttgtt tgaaaaatga accgatcttg atctgttcgt cataagttgg
     3841 aagtgagata ggtgtctttt taccactatc aattgtatag gttccgacgg ttccaatgcc
     3901 tgcaacgtca cggatatact tctgaatttt gttatttatg aacgagtagt aaaagaagtt
     3961 tccgtcaatt ttatgctcat tcttgaacca aatgatgttt ccatccttaa agtaaattgg
     4021 gttatcatca gtgattaata aggggacacc tattgatccg acaccagtta caagcaagtc
     4081 tccttgagaa actttacctg aatttttcga atattcatta tatttttctt ctgaaatgta
     4141 aagataatcg gatggttcct cattttttgc agctgagaca atatcacgag cacgtaaaaa
     4201 tctaatacct gagttagtcc aatctgattg atgaattcgc ttcacagaag taacatccat
     4261 catatctcct aacttacgct cttcccaatc ttccgtaaaa ccaggaaacc ggatttctgg
     4321 tcgtgtatca ttgctcatgt ttgaacacct ccagcgcacc ttgtaaccac gcttcatttt
     4381 caggtgttgt ttgcagggaa gagatagctt ctaaaagttc tttttctagc acttgctgtt
     4441 cttgtcgtat cttcttgatg tcatttccga catgaaccat atcaattggt tcttcttctt
     4501 cgaaagtatc gacatagcgt gggatattta agttatagtc attttctttg atttcatcaa
     4561 aggttgccaa atgagcgtat ttctcgacat cttttctttc agcgtaggtt tcgaggattt
     4621 tttgaatgtt ttcttccgat aatttattct gatttttccc tttaacaaat tcacgactag
     4681 catcaatgaa taatacatct cgagtttgac gattcttctt caatacaatc accgtcgttg
     4741 gaatcgatgt accaaagaac aagttcgcag gcataccgat cacagcataa atactgccgt
     4801 cttctaataa tttttgacga atgacccctt ctgcagcacc acggaataaa accccgtgtg
     4861 gcaagacgat agccatagtc cctgtttcct tcaaatgata gaaaccatga agaagaaacg
     4921 caaaatctgc tttggatttt ggcgctaatt tcccgtagcg attaaaccga gaatcatcta
     4981 agaaggtcgt atctgctgac caattagccg aataaggtgg attcatcact actgcatcaa
     5041 atgtgtaagg ttcatctgtt ggccagtcct tattcaacgt atccccattg cggagattca
     5101 tttcttcggc atctacccca tgtaaaatca agttcatttt tgctagattg tatgtcgttg
     5161 tatttagttc ttgcccatga tatttaacgt tatctggatg agttaagtag ttacgtacat
     5221 ttaacatcaa ggaacctgaa cccatggttg ggtcaaagac actaaagaat cgtcgttctt
     5281 tttgatccaa cgtcacgatt tgtgccatca tatcagaaac catatgaggc gtgtaaaatt
     5341 ctccggcttt cttccctgct tctgaagcaa attgactgat cagaaattca tacgcatccc
     5401 cgatcacatc cccatcgtga cctaaaactt ccacgtcatt caatttcttg ataacttcgg
     5461 taatcgtcac atttcgttgt tgctcatctg ttcctagttt ttttgattgg agatccacat
     5521 catcaaataa cccattaaat tgattgtagg tagatgccaa ttgcacaaag gctttgttta
     5581 gatcgttcaa ttggaacgtt gcttgttttg cttgatccgc taagacgttg aaaagatact
     5641 ctggtgagat cgaatacccc aaaatatcaa cgattgtggc aatcaggtct tcttttgatt
     5701 cttcatctga aagtaattca cgatacaaca ttgtttgttt gcttactgtg tcatattcct
     5761 ctaaggattc atctgctaaa agaaccactt gttccaacaa acggtcggat aaatatttat
     5821 agaaaatcag tcccaataaa taatttttat attctgacgc atccatttta ctacggagat
     5881 tatctgccgc actaaataat ttttgattta attctgcacc caaaaagata cactcctatc
     5941 tcttatttaa agttgctata taccctctta ttttagcata ctccctttta gaaaaagcaa
     6001 aaaaaaaaga aaaaatctta ttcttgataa caagctacgt gacgtatatg cctaacaaaa
     6061 atagaacttt tgaatcaatc ttaaatgaag gtttaaccac agaaccggca tccattattt
     6121 ttgatgaagt tatcgatgac gttgaagata gtaagtatca taaaaattta aaagaacttt
     6181 tttattttgc actcaacaat ggaccaagtt tgttcactgg tatatcagta cctgatagtg
     6241 ataatccatt aattatggca aaaatttact taacaaaatg gtgtaataaa tacatcaggg
     6301 ataggaataa cccagcatta aaaaaacctc ttaaaacatt tggcgaaaaa gatgctgctt
     6361 taactactag agtagcagca aatgctaata ttgatgatca aaaagtattg aatgactact
     6421 tgcgtggaca ttttttatat atgagcgctg agaacatgaa cggaagtatt ttagaggaat
     6481 atttagccga agtgttagaa cctgaaggtt ggatttggtg cgccggctct gtatatcgag
     6541 ctgttgattt ctgttattta ggaacctcac caattctttt acaagtgaaa aataaataca
     6601 atacagaaag cagttcgagt tcagctataa gagtaggaac aacaattaga aaatggaatc
     6661 gtttaaataa gtcgacaaaa atttctggtt tggatagccc tatccccaat tggaaagcat
     6721 taatagaaat gactgaagct agtaaggaac tggcagctaa gttaactgaa aatagttatt
     6781 tagcttatat taatgaaaaa tccacacgag aattatggac actcgatgac taacaatatc
     6841 cttattagta tctatccacg atacatggaa aaaattataa acaaacaaaa aaactttgaa
     6901 tttagaaatt tctatcttac ctcagatcct tataatatct tttggattta cgaaacaatc
     6961 cccaccaaag ctttaaagta taaattgatt gtaaaaaatc caattactaa acttaagaaa
     7021 aatcaacact actttttagg agacgatgag tttgaacaat tagtttaaaa gggaaaatat
     7081 gcttctatca ttacaagctc gtttggtgta agatgggtat aagtcattta tgttcactct
     7141 ccttgtatgc tttagcgggt attacaattt gagtgtaaca taaatggctt ttttatttgt
     7201 ctcgcttaat tatacaaacg gcgaattata aataactcat aaaaaatatt ttgactttca
     7261 aaatcacttt tgctaccaga agttgaggcg aagtgtttta agcatagttg taccctagtt
     7321 tgaccgttaa aaataaataa cgatcgctag agtgcaacta tgtttgtttg ccctaccgtc
     7381 agcgtcggct tggcctctca catccattac cgtcgggatt atctccgtta cataccctta
     7441 caaaatacgc cctgtaagca ttgcatgaaa ccaccgtgca tttgcaccgt atttccgccc
     7501 tagccgttca aatcggtcgc taccaccatt cagaaacacg tgcggtaatg gaaggtttgg
     7561 aggaagctat ccagaccact aacgattgtc cctatcgtcg tacgtttcac tcagatcaag
     7621 gttggtcgta tcaaatgaaa gcttatagaa acaaattgaa ggagtacaaa atctttcaaa
     7681 gcatgtctcg gaaagggaac tgcttagata attcacttat ggagaatttt tttggcttat
     7741 taaaacaaga gatttttcac gggaaagttt ataactgttt cgaggaatta aaatcagcca
     7801 tcgatagcta tatctattac tacaataatg aacggataaa acagaaatta aactggcaaa
     7861 gtcccgtcca atttcgtaaa acaaccgtta cggttgtgta aaaatatcat tttagagcat
     7921 gaaaaaaaga gtggatttct ccactccaaa aaagtctaac ttttgggggt cactacattt
     7981 ttgatccttt ttatttgatt aattatatcc agctggataa taattttggg ggcaacattt
     8041 ctatcttact attatcttcc taaagtagta gactttctta ttgggaaaaa ttacaacaaa
     8101 aaggagtggc ttagttgaca actccttttt gtttacaaat caattggata tctataaatt
     8161 gtattttcta acgaatttta tagacatctg atttgtcaaa ttaagctaga caatatgtct
     8221 ttatctatgc agctcaaaaa tacttccaat ggtgttcgat aatttaatga ttttcttgga
     8281 atatggtttc ttttcgaggc aacggattga ataaaggttt catccgtgtc cctaaagtcc
     8341 attgttttag gtaagccatc ctgcttgagc ccatgcactc ggtactgatg agaaaaaatt
     8401 gatgcctgtt tctcctgagg aatgccaatt ccctgatctt ttacttcaat aatcacgcta
     8461 cccttttggt ttgaaatggt gacaattacc agttttcctt caccatattt gattgcattg
     8521 tcaactaaat tcgaaaccgc ccgataaatc caacgatctc gtccatgaat cagggttggt
     8581 tgatcttcag taaacgcaaa caaaagattc ggataattca ttttgtaggc atcacaaact
     8641 tccgcacaga tcagtgaaac gtctacgact tccgtgctaa gatcctctga gttattggat
     8701 aatgtcaaaa tatcgtcaat actgtcggtc atccgatcta aaagcattaa atttccttga
     8761 tcggcatgtt gccgttctat ttccagattc gtacgcaaaa tagcgatggc atttttctgc
     8821 tcgtgggtca gataactatt taatcgtcga taatcctcca aatgaccatc gaacttatcc
     8881 tttagcttat taaaagccgc atttaatgcc tcgtccttca cagtattttt aggaacttga
     8941 tcaataaacg ttaaatcacg cgtaatcgct aagatttgat tactgtaaat tttgcgtaaa
     9001 accaaccaaa gaataaacgt taataacatg acggcagaac aaataaccag caccatcgct
     9061 ggtaaatatt taaatagttc gtcctggtaa gctttatcta tcatcgaaat agtcataaat
     9121 tgattgttgg aagatttcga agcttccatc tgcgtctctg cttgaaccgt cttaccctct
     9181 tccataaaat aaacatcttc acctaaggta gatgcgatac tgattccctc caaattattt
     9241 ctcagaaaga agaaactcac taaaatcacg cctgtcacca acagtgaaaa agccagcatc
     9301 gttaacgtca ttttaaaatt taatagcata gctggtaccc ctttgctcga atggttttta
     9361 agacctcttt ttcagctgct tcagccagtt tcttttttag acgagccaga tgcactcgca
     9421 gaaccgacga aaaaggatca aaatcttcat cgtagacatg ttcagaaatt tcctcagacg
     9481 aaacaaccgc tggatgacgc tgagcgatgc acagtaagat atcaaattcc ttgggtttca
     9541 ggaccacctc tgtcttttga tagctgaccc ttcgacctaa tggatcaatc ttcaaagcac
     9601 caattgaaat ctgcggcagc gcacgtccat gataacgacg aataaccgcg cgtattcgtg
     9661 cttttaattc tagtaactca aagggtttga ctaaataatc atctgctcca aaatccaagc
     9721 cttttgctcg ttcttcaatc tcatcacgtg cagtaataat gataactgga ctttcgactt
     9781 cttcagcacg gagactttgt aaaacagata atccatcttt tttaggcaga tttaaatcta
     9841 gtagtatccc atcgtatgaa ttgatgaagg ccttctcttc tcctgactcg ccatcataag
     9901 ctacatctac agaaatattc tcctgctcta ggcccttttt tacggataaa accagctcac
     9961 gattgtcctc aaccaccagt acacgcatgt atattcactc ctcatacgga tttagtaatt
    10021 acacttatgc tttttagcat agtacacgct cttcttcaaa gtccacgttc tttttgtgaa
    10081 gatttgaaga aattcataca ttctgttaaa aaacacataa ttaagaatga ttttactgtt
    10141 tgaaaatacc tagaatagaa aaagactgga acctttcctc cagtctcact actttctatt
    10201 tgcttggttt tcttgtatat acttttcaaa gttcgttggt ttatgaccaa taagcctcgt
    10261 tagaatactg aaaattgcca tcaagtcatt aagtaccaca tgtaagaaaa tctctctaaa
    10321 gaaaaacccc ttcgttttaa ctcacttact attttagaat tcgttcccct aataatgcag
    10381 catgatttta cgctttatgt ataaaaaaag actgcaagca attttgcttc acagtctgat
    10441 ttcttatgat agtgagcttg ttaacacttc caccggtgta gcagtttctt tcaatacggc
    10501 atttaattct tcaatatttt tacgcccttg cgtattcatc cactccatcg ctgcttcttt
    10561 tccatcaacg atatagcttt ctacaccatc tgcccaagtc gcacggccgc aaagtacacc
    10621 attgaaggtt gagccggcat ttttcgcgaa gcgcagtgtt tcttggaaca actctgcttt
    10681 tacaccggcg cttaggaaaa taaatggcag gttagtggct tcagcctgtt ccttgaagaa
    10741 gttagccgct tgtgcctttg tgtacacgac ttcgtctgtc ccatagcctt ccacataatt
    10801 catattgacg ggtacttcca cctttaacac atctacgcca taacgtggat cagaaaattc
    10861 cttcatcata tcgttgactt tatgaggttt cttaatggcg tattctttcg aagtcgcatc
    10921 agagatgttc gcgtcataag aaacaagctc caagaagaag ggtaagtttt cagcctgaca
    10981 ttctgagccg attcgttcaa taaaggcatg tttttgatta ttgatttctt ctgcttcatc
    11041 gacatcatag taaagtagga acttacaagc atcggcgcct gcttctttta aacgtttaac
    11101 tgaccagatc gataataaat ctggtaaacg gccgggagtt gacgcatcgt aaccagtttt
    11161 ttcataagct aacagcaagc cggcattttt tgctctaacg tcagctgctg gcaagccata
    11221 ttcgggatct aataaaatcg atgaggcata tttcgttaat tgtgaagaaa caatttcttt
    11281 aaattcaacg atttctgatt cttctgcttc gctgcccttg aattttgtga tcatcttctt
    11341 caaagccccg cgttgatcaa ttgctaaggc cccaatcact ccatcttcat tgcatagttg
    11401 cttgatatat tgacgttttt cttcgttcat ttccgtatcc tcctacactt tctccactaa
    11461 gatttgatca tatagttttt gataattggc taattggaca tagccagtcg tcgcttcctg
    11521 agcattcagc attcctaaaa cattaccctg ttttaacacg gcttcatccg aaagaccttg
    11581 ttgaatcgca gcggtcaatc cggcaacggt cgcatcacca gagccaactg gattgacgac
    11641 atcaatcttt ggaattgtca cgcaataaaa agtctcgtga tgtttggcaa aggctccgtc
    11701 gccccccatt gaaaccacta tccattcaat ttcatcaaac atcgaatctg acaaggcggc
    11761 ttttaaatct gataattctg ttgagatttc tcgtcctagc agaccagcca gctcttcggt
    11821 attgggtttt attagcttag gctttacact gcctcgcaac acttccctta atgctgtacc
    11881 tgaacaatct aaaacgacag gctttggctg acaaacttgc agcatttgtc gataaaaatc
    11941 agcaggcaat ccttctggca agcttcctga aaaagaaatg atttcggctt taacagctaa
    12001 tttttcgaaa tgttgcaaga acgcccttgc ttcttgttca gtaataacag gtccgttttc
    12061 taggatttcc gtctgttttc cgtcatgtaa aacagcgatg caattacgtg tctctccgct
    12121 gataggtaaa aagtcatgag caatctcatt ttgagtcagg tcctttttaa tctcttcacc
    12181 taaaaaaccg ccgattaaac cactagcaat cacctccgta tctaattgct tcaagatacg
    12241 tgtcacattt aggcccttac cgccagctgt tttgcgaaca tttcttactc gattgactgt
    12301 gtcgagcttg aaggtttcta atggataaga aatatcaatc gatggattca tcgttattgt
    12361 taaaatcatt tgtccacccg cttaatcgtg gtattcgcca cgatcccatt tctcgataaa
    12421 ttcatcgaag aaatgcggat cagactgctt gtcactggct ttttcgacgt gatcgatttt
    12481 ggcgatcaat tttttgtttt cttcagtttc ttgataatcg gcattgatga aggcctctaa
    12541 tatgtcacag atcaaaaatt ctcccgtaat ccgagcgcca aagccgatca cattggcatt
    12601 tagttgttcc ttcgcataga tagcagtcgt catatcgcga accagcgcac tgcggatacc
    12661 aggtacttta ttgacagcgt tgttaatccc aacacctgtt ccacacaaac aaactcctag
    12721 atctgcttct ccgctagtca cggcctctcc gacttttttt ccgtagatcg gataatgggt
    12781 tctggtaaag tcataggtcc ccatatccaa cacttcatgt ccttttgatt ttaagaaatc
    12841 tgatactgcc atttttgtat ctgtgacgat gtggtcacag ccaattgcga ttttcatttc
    12901 attcatcctt cctaattacg ccattttatt taacatgtcg acacgtactt gatgacgacc
    12961 accatcataa tttgcttgta gaaattcctt aacaatattc aacgccaact catcgccaac
    13021 aatttttgat ccaagtgtga tcatccgaga attattatgt ttacgggtca tatacgcact
    13081 gcgctcatcc gaaacttctg ctgctaccat tcctttaacc tttgttgccg tgataaagct
    13141 gccagcgccg tagccatcaa ccgcgattcc taaactacct tcgttttcat tcagatcatt
    13201 tgcgatcgcc aaagttgatt ccacaaaatc ttctgaagcc gtttcactct tatctactac
    13261 tgtaatacct tctttgatca attttttctt gattaattcc tttaattcca tgccgtcact
    13321 gtctgaacca attactacac gcatttttgt ttcctcctat tttttactga atgattttag
    13381 tgaattgctg ataataactc ttcagctcgg ctgaaagagt agaatccgta aataatttgt
    13441 cgatcttgcg caaatcatag aaattataaa aatcgctatg gtcgaatttg tttgcatctg
    13501 aaaccaaaca tttttctgcc gcattatcta aaacaatttt ttgcaaggta ccttcttcaa
    13561 tactgaaatt cgaaacctcc gaacccttca atccattcac tccgacaaaa gccttcttaa
    13621 taccgatttt ttgaacagct tcattggcaa ttgaaccgac aaaggctcca gtcttttccc
    13681 gatatagccc accgatcaaa tacagttcaa atctttttat ttcacttaat agattgaaaa
    13741 ccggcagact attggtaacg attcggagag atgacggctt gatgtatcgt gtcattaatt
    13801 cgatcgttgt acccgatcca agaaaaatcg tgtcgtcttc ttgattgata tgagctgccg
    13861 catttttagc gatgtattcc ttctcttttg tatgcagctt ctttttttca gtatgagaaa
    13921 gctcatttgc ttctatagaa gtcgacattt ccagataatc gattttttgt gcaccgccat
    13981 gaacgcgtaa aagctttttc ttctgcgcga gttcctctag atctcggcgg atcgtcatat
    14041 ctgaaacatt cagtacttcc tgaataaatt tgactgttat cgtcccatag cgatcacaca
    14101 tcgctaagat cgaacgctgt cgttctgttt tcagcatatt ctgtacctcc acgaccttat
    14161 aataaaacat ttacgaacat ttttcaacac atattgttcg tttattttcg tttatgcgtt
    14221 gacaatgatt ttgtaagcgg ttatactcaa cttgtaaaca tataaataaa cataaacgaa
    14281 ctaataaagg agctgttttg tgtgaatgaa ttctttttca aagacactgt ctatgtttct
    14341 gacaagcagt cgcaggaaga agtgttcgag gaagtctatc aagatctgtt aaagaaagaa
    14401 ttggtaaccg aagatttttt ggagaacctt ttagaacggg agcgtaatta tccgacggga
    14461 ttagatctct cacctgtctc taaaatattg ccagatatcg ctatccctca taccgaaagt
    14521 gagtttgtgc gaaccacccg tatcattcca atcaaagtaa aaaatgaaat ccctttctac
    14581 aacatgatct cgccaagcga tcagatttcc gcttcatttc ttttcatgat cttgaacgaa
    14641 aatggtgaag cacaggctgg catgttggct aatatcatgg atttcattaa ttctgttgac
    14701 gaagaaaaac tgttttcgtt tttcaaatta gaggacacag aagcgattta tcatttttta
    14761 gaagaacatt tttatatata gaagtctctg cctaaccgca ggataaaaaa taaacttatt
    14821 ttaaggggag aattattatg atcaaaattt tagcagcatg tggagcaggc gttaattcaa
    14881 gccatcaaat caaaagcgct ttggaaactg aattgaaaaa tcgtggctat caagtcacct
    14941 gtgacgccgt tatgatcaaa gatatcaatg aagaaatgct gtctcattat gacattttcg
    15001 cacaaattgc caacaccgac ttgggttttg acattaacat ccctgtcgtt gatgcaggtg
    15061 ctattcttta tcgtattcct gctatggctg agcctgttta ccaaagtatg gaaagcgcaa
    15121 tccaatcatt aaacaaatag gactaaagaa aggaaaagca tatgaataca attattgata
    15181 ttgccaataa agtatttacc cccctaatta atttaggtgc ggcaccgtta atgaccatcg
    15241 tattaactgt tattgcttta ttttttaaag taaaaccgtc acgcgctctt gaaggcgggc
    15301 tcaaattagg gatcgccctg actgggatcg gagctattat tggaatttta actactgctt
    15361 tttcagacgc tatgacagct tttgttgaac gcacaggtat ttcattaaac attacagatg
    15421 tcggttgggc cccactagca accatcacat gggggtcgcc atacacgctt tattttttac
    15481 taattatggt gatcgtcaat attgtcatgt tagcaatgaa aaaaaccaac acattagatg
    15541 tggatatttt cgatatttgg catttagcaa ttgttggttt gttcgcgatc ttctgcggtg
    15601 ctaatctgtt tattgcttct gcattagtta ttttcattgg tgtattaaaa atttggaact
    15661 ccgacttaat gaaaccaacc ttcaatgact tgttaaatgc gccagacagc aacccgatga
    15721 ccacgactca tatgaactac atgatgaatc cgattattat ggttttcgat aaaattttcg
    15781 acaaaatctt cccttggttg gataaatatg atttcgatgc tgccaaattg aatagtaaaa
    15841 ttggcttctg gggatcaaaa ttcgcgattg gaatttattt ggggattttt gttggattac
    15901 tagcaggaca aacaccaacg caaattttct cgctagcctt taccgctgcc gtctgtttgg
    15961 aattgttctc tttgataggc gcttggttca tcgcagccgt agaaccgtta tcacaaggga
    16021 ttaccgattt tgccaataaa cgactaaaag gacgtaccat caacataggt ttagactggc
    16081 ctttcatagc aggccgcgct gaaatctggg ctgccgctaa cgtattagca ccaatcatgt
    16141 tgctagaagc catcatctta ccaggaaata aattattgcc actaggcgga atcatcgcaa
    16201 tgggtgttac cccagcacta ttggtcgtaa ctcgtggaaa attaatccgt atgatcgtaa
    16261 tcggcgcgat cgaattacca ttattcttat ggtccggcac cttgatcgcc ccgttcgtta
    16321 ctcaaacagc taaagcagtc ggagcctttc cagccggctt gaacgggtcc gccatgatct
    16381 ctcatacaac gatggaaggc ccgattgaaa aattcctagg ttatttagtc ggtaatgctt
    16441 ctcaaggaca aatcgaattt gtgatatacg ctgccttagc attaggtgca taccttttaa
    16501 tcttcatttg gtacgctcgt caaatgaaaa aacgcaacgc tgcatacgca gctgaaaaag
    16561 aagagaatgc atctccggca acggcgcata atatgactta tgcagccgaa gctaaataag
    16621 ctaaaaacac cccgaatgtc tttagacttc ggggtattat tttacctata aaaaataaat
    16681 agatgtaaaa tccatttcaa aaagcatagc ccatctcttg ccacacccaa taaagaaatt
    16741 atcaacgttt atgctttttt taactccttt aagcttgcct ttgttggtat tcctcaacga
    16801 ttctttttgt gttcttgttt gttttgtgct ttttgacttt ttgttgttta attgcgaatt
    16861 ccttgatttc ttttttcctg aatcgcttcg catcccgcgg ttatgcgtgc gatttcatca
    16921 ttgctttttc tgatctaact cgtttcatgt caaagagctt cagctgactt agtgtccatg
    16981 gttgttttga tatccctttt cctccttaat aaagcggtga ttgatactgc tattttttcc
    17041 ttcacataaa cataacatcc caaaaaaatc gactatcttt tttcaaatgg tgtattcaag
    17101 tattcggagg aattagtaat gatgcaccct aaaaataaca taattcgctt aaattcaagg
    17161 ctaccaaaaa actaccaagt tctaattatc tagagtttgg cagtttctca tttacacaac
    17221 atatttttct tctttttcga ttttttcata gtgcttttgt tcatcatcat ccgttataac
    17281 tactgttaca tcattcagat tataatatac tgaaaaatct cttttcccaa ttttcgaatg
    17341 atcaatcaat aaaaagcgtt caacagaatt attcagagcg atctgttgag tttggccctc
    17401 ttcaaaagtc gaagtcataa ttttctgttc atttaaagca ttacagctga aaaatgcttt
    17461 atgaaaattc atatcctgta gcgatttatt cgtgatttca ccaacaaagc attgagtcgt
    17521 ttcccgcatc tcgccaccta atagatagat ttttcgattc ccttttcgct tatttaattc
    17581 ttcaaaaaca ggcaaacagt ttgtaacaat ccgcagtgaa tccccttgaa tgatttttga
    17641 catgagctcg attgtagttc ctggtccaag aaatatcgta tcaccatcat tgatcatttc
    17701 attcgccttt tccacaatcc gctgcttttc ttcttgcagc ataatctgtt tttctctatg
    17761 agaaagctcc acatgccgat aaaaatcctt caatttcgct ccgccatgga cgcgttctaa
    17821 taatccttgt ttctccattt cgtccaaatc acgtcggact gtcatatccg aaaccgaaaa
    17881 cttctccata atatcgctga ctcgtactgt accgttaacc tctaataacg acatgatcgc
    17941 ttggaatcga tccgttttta acatttcatc gcctcttttc tttttaagac tatcaaattc
    18001 ttaagaaaag tgctagaaca agttttctta agaagccttc tgcaggaagg aaagcaaaat
    18061 ttttcagaat ttgcaagctg ctattctaaa aaataaaacc gaacgagcat gactgtcata
    18121 gtgacagcct tgttcattcg acttttatct ttaataaatc attttattta atctgaagtt
    18181 attgtgcaag ttcaaaaaca aagtagttaa tgtctcctcg atatttcgaa atcgaatact
    18241 caacgggcag acgttctagc gaaaaaccct gtgtatggaa atagaacatt ggatctcctt
    18301 cttccacatc taacaaatcg ctgatcgtat catccgcttt gattacttct agttttctgg
    18361 tgaccgtttt aattggataa cccatttctt caaaaacttg atacattttt tcttttctga
    18421 tttgtcaaat taagctagac aatatgtctt tatctatgca gctcaaaaat acttccaatg
    18481 gtgttcgata atttaatgat tttcttggaa tatggtttct tttcgaggca acggattgaa
    18541 taaaggtttc atccatgtcc ctaaagtcca ttgttttagg taagccatct cgtctaagca
    18601 agccgtttga gtgctcgtgc tcatttagcc ctctttgcga aggtgttcct ggatctgcga
    18661 aaaagatggc aatatcgttc gagttactta tagatcgcca gttggaaaat tcttttccac
    18721 aatcaaatgt catggattta aacaagtgtt taggcatgga tttgaaccaa tcatttaacc
    18781 tgttttcgat atcttcggct cgtctgcctg tcggtttcaa agtaataata accttggata
    18841 aacactcaac caatgtaata acagcgctct tgtgtttaac tcccacaatg gtgtcgcctt
    18901 cgagatgacc aaattcttta gtcaggtgtg gatagagact gttccgttca tgaatcgttc
    18961 tgcgaaaggc ctgttttcca cgcttttctt gatgattatt cggtttacgt ttccccttca
    19021 taggtaacga ctgtgtgtca aaaaggcctt ttttaaacat ccgatagagt gtacgaacag
    19081 aacaatcaat tggaaactcg ccacggccga taatcacatc gggtgaccac ccttgagcta
    19141 ccttttcttg aacatatgtt gattgttcat cagataaaac ggttgaacgt cgtccacaac
    19201 gagatttatt ttgtttgtat tgacggtaat aatctagcac ttttttcctt gtttgagaaa
    19261 cgaataaaca ttataaatgg tttgtctaga gcgatttaaa ctttgtgcga caagagcgac
    19321 aggtttctcg atattgaaat aagactctat gattactagt tcgtctgttg taagatgggt
    19381 gtaggtcatt tgtactcact ccttattaat tctttggtcg gaactatttt gagtgtagca
    19441 caaatgatct ttttagttgt ctagcttaat tttacaatcg acgcctcaaa aagaataccc
    19501 gaaatttaaa cagcaaaaaa agatagcgct taccattgtg tttaagataa aacgctagct
    19561 aagttggcta gcgctttatt aatctaagca agctctaaaa ttgcttcaat acaaaatcaa
    19621 ctgctccttt aggatcacgt gttaaattga tatattctgc acctttagtt gcaattactt
    19681 ttatccctaa tttgtctgca tctttacgga gatcatccaa ataggaattc acttgaggtg
    19741 ctagaacgat taaatcaaaa tttttcatca tatcataatg agaaccataa gctcctgctt
    19801 tagcagaaag attaacacct gtctcttttg cgccctcttc taatgcatta gccaacattg
    19861 cactcgttcc ggcaccagca caaagtacta aaacgcgctt tccttcacca accgatgctt
    19921 ttgcttcatc aactataact gccgcagttg caccaacttc ttggatttct tcgaccaatt
    19981 cttcttttac ttcttctgca gcaatgtttt ttaactcttg ttctactaat tcagaatcat
    20041 aggccttaac aaaaggtaaa taaataatcg catcgacaac aatcaatact accgctaata
    20101 caatcgccaa cacactaaac ccggttccta aaaccaagcc aatcggtgcg ggcgtcgccc
    20161 aaggcaatac ggatataaag ctattcatcc ccaagacatc gacaaaaagt ttgaatatcc
    20221 atacgttagc gattggtgct aataaaaatg gaattagaaa gtacggattc aaaataatag
    20281 gagtagcaaa tagtaacggt tcatttaccg caaacgaaac tggaataaat gaagctttac
    20341 caactgcttt caattgtttt gatttagcga acaacatgaa cagaaaagga acaactagtg
    20401 tcgctccagt accacccatc gttccaacaa agtttcctag accaactgtt aatacttttg
    20461 aggcttgtgc accgttttta aataattcaa agttagtttc tacgttcgca taaataattg
    20521 cagcaatcgc tggttcaaca atcgatggtc catgaacacc aacaaaccag aacattgcca
    20581 tggctcccca aatgattgca ataccgacat atccatcagc agcactaaat aatggctgca
    20641 ataattgaat tacacctgct gcaaatgatg ttccaaaaaa ttgacgaact cccgtatcaa
    20701 tcaaaacggc aataaatact gcaattgaaa aggggataat atctctaaac atctgtgtga
    20761 ttgttccggg aacttctttt ggcatacgga tcgtcaaatc tcttttaaca cagaatttat
    20821 acacatttac agtaacaaac gcagaaacga aagctgctaa taatcccttt gtccctaaat
    20881 aatcagtagc tatcccacca tcaatttgat cagctgccat cattacaaaa ccacaaattg
    20941 acgctaacat aacagaaact tcattaatct gcttacctga atcagataat tgacggttca
    21001 ttgaattagc taaacatcga gctgttgtag caacaaccaa aagaccaact aatcccattg
    21061 aataaccata aactttccaa agccacgtcg agaatgcctc tggtaacgtc acgttaaata
    21121 cttctggaac agccgcaatc ataataaaaa tacttgaaaa tagaatagct ggcatagcag
    21181 ttaaaaagcc atcgcgaatt gcccctaaat aaatattacg tgagattttt tcaaaaaacg
    21241 gttttccctt ctcaatttgc ttaataatcg tattcatttt tcttctccta tgctttagca
    21301 aatttttctt tgtacaattc aacaaaatca ttgataagtt ctcttaatag aatcgttgtc
    21361 attaaatggt cttgcccatg aataaaaatt acgcctactt ccatttcgtt ccctgacgct
    21421 tcttcagcta aacatttcgt ttgtgagtta tgcgctaagg ttaaattttc atcagcatcc
    21481 gctaataatc gttcaacatt atcaaaatta ccttctctca cttccttcaa cactgttaat
    21541 aatgaacttc tggcatctcc tgcataagca acaatttcaa atcctaataa ttgcaattct
    21601 ttcctatcca tttccttgcc ctcctaacta tttttatatt atcgtttttg tttcgctaac
    21661 aaatttatac caatatgcag actctttcgg atatcttttt tgtgtatcga aatctacata
    21721 gaatagtcca taacgcttag tgtatccatt aacccaagag aaaacatcca ttaatgacca
    21781 aatgaaatac ccctttacat taactcccat tttgatagca tcggaaatcg cattaaaatg
    21841 ctggcgtaaa taatcgattc gatcatcatc tagaataatc ccatcttcaa aattatcctt
    21901 ataccctagc ccgttttcag taatgtatat tttattataa tttggatatt cgtcttggat
    21961 tcgtacaagt aaatcatata acccttttgg ataaatgatc caatcccaat cggttttcgg
    22021 aagatcctca cgcacgattc tctcgccaat ccctttaatg caatagactt ggctaccttt
    22081 ttctccggta gtgttgtagt gaagtgtgct ttctcctttg tactctttaa cccaatggct
    22141 accatagtta ttgataccaa gaaaatcatt tcgatttgct gcttttttca tctctctaaa
    22201 atcattagaa tcaatatctg cagattgccc atttgctgct aaaatctcat taatatgtgc
    22261 cattgtttca tctgagtaat accctaaata agtagcatct aataagaact taattgataa
    22321 cgcatcatct aatttcgcag catgcttatc ttcttctttg tcacttgctg gatattttgt
    22381 ctctagcgag tgaacaacac caatttcccc tttatatcca ccatctttaa aaatgtttac
    22441 tgtctttgcg tgcgcataca tcatgttgtg taaacaagtt acagtcttct ctatatcaaa
    22501 ttgaacttct ggaggaaacg atccgatcaa atattgattt gaagcaactg gataaatctc
    22561 attaaatgta ctccaataat gaacctcaga aaattcatcg aaacagaact gtgcatagtt
    22621 tacgaaatgt tcaacatttt ctcgatttaa aaaatctcca ttatcaaata gttctttagg
    22681 ggtatcaaaa tgatgaagag ttacaaaagg aataatacca tgcttattca attcagcaaa
    22741 taccttatga taatattcca ctcctgtttg gttcacttcc ccatagcctt ttggaaaaat
    22801 acggctccat gcaatagaca tcctaatact atcaatttta aattttttgc ataattcagc
    22861 atcaactgga tattgattat aaaaatcact agctggatca gcagtatagc gtcccttttc
    22921 tacaaggaaa gtatcccacg ctacttgccc ttttccacct tcttttgtag agccttctgc
    22981 ttgataagct gccgtagctc ctcccataat aaagtcttct ggtaaagttt tataattcat
    23041 tttaatctct ccttgaaaat tagtttgatc aatatgccaa taatccataa atcacctcag
    23101 cataatttta acatgtataa gtaaattttt ttacacaacg aaaaaccgac ctcgaatatc
    23161 tattttattg cgatatacaa gcacttatat cccttgatgt aaaataaata taccattcaa
    23221 aaaccgtttt gtaaacgttt ttttgtaaaa taaatttatc tttttactaa tttaatttac
    23281 acgaaacaag aaaacggact gattcccact aaaatcaatc ccacctacta atcattctcc
    23341 aagtggctat ccattatcga ttcaatcttc tctgttaatg gattaggctc tctgctgata
    23401 tcagagtttc gtttcctgct agaaaaagta atttaagtta ctaaaagagc agtttcgatt
    23461 tactttaaca aagaaaagga ttattatatc cctaggagga tgaacaatgg ttactattag
    23521 agatattgca aaaaaatctg gttactcaac ggctactata tcaagacttt ttaaaggtga
    23581 tgagtcactc tctattacat ctgatactaa aaacaaaata atcgttacag ccaaatcttt
    23641 aggatatgat ctgacaaaaa ttaagactac tctgtacaag attgccattc ttttttggat
    23701 ctcagaggaa cagttaaaaa aggatgtcta ctatcaacat gtcaaagatg atttgaaaaa
    23761 ttatagtaaa aaagctaatc tagatttgat tttcatctct aaaaacgatg gtgtaaaaag
    23821 cattcctgac gatattactg gatttatcgc aataggacaa ttgacaaaaa aggaattgta
    23881 ctacttaaat caaaaagggt ataagggggt aatccgagga ataaacccat tacctgaaca
    23941 cttcgatact gtagaacctg atttccacga aatggccaga caagcaatta actactttat
    24001 taatgaaggt ttcacaaaaa ttggatttat tggaggaaat ttctttaacc tagaaacggg
    24061 taaagaagag attgatagcc gtgaatatgc cttccgatat catttagaaa agagcggact
    24121 actcaatgaa aagtatattt tctcccaagg atcatttact gttgacgaag ggtatcaatt
    24181 aactcttaaa atgaaagctg agttaaatga agatatgccc gaggcatgtt tcataccatc
    24241 tgacactata gctgccggcg ttcttcaggg ctgccatgaa ttgggtatta acatccctaa
    24301 agatatggca gtaataagta ttaatgatga cgacatcgca aaattcgttg caccaccact
    24361 tacaactttt aagatagata tcgaagagat agctaaaacc tgtgtagatt tattaagtga
    24421 tcaactggta tattcgcggg acatatcaaa gaaaacatta atcggcgcaa aattgaccgt
    24481 taggaaaagt tttatcccta aagaagaggt gtaaaataag taattgaaac ttatctttaa
    24541 aactataatt gtgtccacat agtagccgag caatcgggtt tatgcctatt atgttttcca
    24601 taaataattt gatggtgata ttttgacgta aatacaatat gattttttca tcgccaaatt
    24661 gtgcgcgcta atttttgttt atcattcaga acatttcctc ctttttatct aagtatgagg
    24721 ttggataaac gcttcatctt agtatcttgg gggatttttt atctcgttgg gaatatatca
    24781 agcatcagag cgcgcaaaaa gaactggtcg aaaaatcaat ctaccagttc aatctcattc
    24841 actgtgatac tatgttttat acagataacg ccatattata aaacaagttg tattccgcaa
    24901 atgggttcta ttcaactctt aaactatcta taaaagattc agccaactcc attagcctct
    24961 cgtaatttag ttggtgaaca gcactttttt caaaaaattt tgaaccaatt cctacataag
    25021 aggctccatt gttcaaatag ttttgtgcat tagtctgatc tacccctcct acagccatca
    25081 aaggtaaact tcctaaaggg gcttgcactt gattgaaata ttccggtcca agacagttgg
    25141 cggggaacac cttaacaata tcagcaccca tctcaaacat tttgaatatc tctgtgggcg
    25201 acatggcccc aggaattgtt agaaggttct gttgcgaagt ttgaaaatca aacgcgccct
    25261 ctctgaactc caaatatctt aggctgtta
//
LOCUS       DACDIE010000038        24312 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.038, whole genome shotgun sequence.
ACCESSION   DACDIE010000038 DACDIE010000000
VERSION     DACDIE010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.038"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..1154)
                     /locus_tag="IV250_001701"
     CDS             complement(<1..1154)
                     /locus_tag="IV250_001701"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HAP8768985.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            1339..3780
                     /locus_tag="IV250_001702"
     CDS             1339..3780
                     /locus_tag="IV250_001702"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAP8768986.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(4110..4814)
                     /locus_tag="IV250_001703"
     CDS             complement(4110..4814)
                     /locus_tag="IV250_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8768987.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(5001..5246)
                     /locus_tag="IV250_001704"
     CDS             complement(5001..5246)
                     /locus_tag="IV250_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8768988.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(5263..6114)
                     /locus_tag="IV250_001705"
     CDS             complement(5263..6114)
                     /locus_tag="IV250_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP8768989.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            6280..7284
                     /locus_tag="IV250_001706"
     CDS             6280..7284
                     /locus_tag="IV250_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAP8768990.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            complement(7525..7881)
                     /locus_tag="IV250_001707"
     CDS             complement(7525..7881)
                     /locus_tag="IV250_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP8768991.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            8249..9436
                     /gene="tuf"
                     /locus_tag="IV250_001708"
     CDS             8249..9436
                     /gene="tuf"
                     /locus_tag="IV250_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP8768992.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            complement(9624..12215)
                     /gene="mprF"
                     /locus_tag="IV250_001709"
     CDS             complement(9624..12215)
                     /gene="mprF"
                     /locus_tag="IV250_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP8768993.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(12241..12960)
                     /locus_tag="IV250_001710"
     CDS             complement(12241..12960)
                     /locus_tag="IV250_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8768994.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            13280..13801
                     /locus_tag="IV250_001711"
     CDS             13280..13801
                     /locus_tag="IV250_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAP8768995.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            13860..14720
                     /locus_tag="IV250_001712"
     CDS             13860..14720
                     /locus_tag="IV250_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAP8768996.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLCGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            14827..17220
                     /locus_tag="IV250_001713"
     CDS             14827..17220
                     /locus_tag="IV250_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP8768997.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            17402..19243
                     /locus_tag="IV250_001714"
     CDS             17402..19243
                     /locus_tag="IV250_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAP8768998.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            19267..20376
                     /gene="rpoD"
                     /locus_tag="IV250_001715"
     CDS             19267..20376
                     /gene="rpoD"
                     /locus_tag="IV250_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAP8768999.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            20596..22695
                     /locus_tag="IV250_001716"
     CDS             20596..22695
                     /locus_tag="IV250_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP8769000.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            22891..23754
                     /locus_tag="IV250_001717"
     CDS             22891..23754
                     /locus_tag="IV250_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP8769001.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            23859..>24312
                     /locus_tag="IV250_001718"
     CDS             23859..>24312
                     /locus_tag="IV250_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP8769002.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKE"
BASE COUNT         8127 a         4299 c         5037 g         6849 t
ORIGIN      
        1 tatattttgt agtaacgaat atcattttcg atctttgtaa tcgaaaaagg tacatctgaa
       61 gtatattctg cacattctcc gtgttcagga agcgtctcac tgaaaatcgt ttcatattcg
      121 tcaacagtga tccgtgttcg agaatctagc atctcttgat gagattgtgc gaagagatat
      181 tcttgatagt tttcttccag ataacctgta aaaaattcac ttactgcacc agaaccgtag
      241 ctgaatagac cgatgcgatc tcctggctgt agtgatttcg aattttccag tagagaagtt
      301 agccccaggt ataatgaacc agtgtaaaga ttaccgattc gtcggctgta acggatgctt
      361 tcttcatagc gagccataag acgttcctga ttttcttcgt ctgtttggtc taatacgctt
      421 tgcaatgcct tttttcccat cttagtatac ggaatatgga aagcaatcgc ctgataatct
      481 tcgagtcctc gacccgacaa ttctttatgt cgattccaaa ctttttggaa tgattcgata
      541 tacgtagaat tagataaagg accatcaaca acaggaaatt cgctataatc tggacgccag
      601 aaatcataga tatcttctgt cagaaatacg ctgtcgtctt caatcgataa aatacgcggg
      661 ttttgagtga tcatcatcgc aacagcaccg acaccttgcg tcacttcacc accgcttgcc
      721 aagccgtaac gagcaatatc acttgctatg actagtactt ttcgttctgg atgatttttg
      781 acatattctt tcgccatgtg caaagctgcg gtgccaccgt agcaagcctc ttttacttca
      841 aaagaacgag caaaagattg gattttcagt aaatggtgga taattaccgc actcgctttt
      901 gaatgatcaa tcccggattc tgttccgacg atgaccatgt cgattagctc acggtctttt
      961 tctgttacga ttttgctggc agcatttgct cctagtgtaa tgatgtcttc atttgcacga
     1021 ttgactgcca tttggtcttg accaatccct atatggtatt ttgcaggatc atccccacgg
     1081 ctttctgcca actctgtcat atctaaatat aaattaggaa taaaaaagga aagacgatca
     1141 atccctattt tcatttcaac acctctttac ttatgataaa aaacttactt tactaaggta
     1201 tcataaaaag attaaaagaa atataaaaaa gagaatattt aaggattgtt aaaatttaat
     1261 cttagaaaaa aataactgga acaagagcta tttgttttta cagcaaagaa tggtatattt
     1321 ttgtaggagg tggcttactt gaaagaagtc gttatgatag atgctgcaag aacgccgata
     1381 ggaaagtatc gcggaagttt aagtccattt actgcagttg aattaggcac acttgtgact
     1441 aaaggattat tagataaaac aaaactgaaa aaagataaaa tcgatcaagt aatatttgga
     1501 aatgtcctgc aagctggtaa tggtcaaaat gttgccagac agattgcttt gaacagcggt
     1561 ctccctgtag atgtcccagc aatgacgatc aatgaagttt gcggatctgg tatgaaagca
     1621 gtgatcttag cacgccagct gatccaatta ggtgaagcag aacttgtgat agccggcgga
     1681 acagagagta tgtctcaagc acctatgctg aaacagtatc agtcagaaac aaatgaatat
     1741 ggtgaaccaa tttccagtat ggtcaacgac ggattgactg acgcattttc aaatgcacat
     1801 atgggattaa ccgcagagaa tgttgcaaca caattttctg tgagcagaga agagcaggat
     1861 cgctatgcct tgtcgtccca gttgaaagca gcacatgctg tcgaagccgg tgtattttct
     1921 gaggagatca tcccagtcaa gatttctgat gaagacgtgt tatctgagga tgaagcagtt
     1981 cgtggaaata gtacattgga aaaactgggc acgttacgta cagtattctc agaagaagga
     2041 actgtaacag caggaaatgc ttccccgttg aatgacggtg cctctgtggt gatccttgca
     2101 tccaaagaat acgcagaaaa taataatctg ccttatttag caaccatcaa agaagtagcg
     2161 gaggtcggta ttgacccaag tatcatggga atcgctccaa tcaaagccat tcaaaaattg
     2221 acagatagat caggaatgaa tttatcaaca atcgatcttt ttgagatcaa tgaagcattt
     2281 gcagcttctt ctatcgtagt tagccaagaa ctgcagttag atgaagaaaa ggtcaatata
     2341 tatggaggag caatcgcatt aggccatcca attggtgcaa gcggtgcacg tatcttgact
     2401 acattagctt acggattgct tcgtgaacag aagagatacg gtattgcttc attatgtatt
     2461 ggtggaggtt taggcttggc agtcttgtta gaagcgaata tggagcaaac acataaggat
     2521 gtgcaaaaaa aaaagtttta tcagcttacg ccatctgaac ggcgcagtca attaatagaa
     2581 aaaaatgtat tgacacaaga aactgcgttg atttttcagg aacaaacact ttctgaagag
     2641 ctctccgatc acatgatcga aaaccaagtg agtgaagtgg aaatcccaat ggggatcgcg
     2701 cagaattttc aaatcaatgg caagaaaaaa tggattccga tggcaacaga ggaaccatct
     2761 gtcattgcag ctgcaagtaa tggtgcaaaa atctgtggaa atatctgcgc agaaacacct
     2821 cagcggctga tgcgagggca aatcgttcta tcaggaaaat cggagtatca agcagtgatc
     2881 aatgcagtta atcatcgaaa agaagagctg attctttgtg caaatgaaag ttatccttca
     2941 atcgtgaaac gcgggggcgg tgttcaagat atctctactc gggaattcat ggggagtttc
     3001 catgcatact tatctattga ttttttagtg gatgtaaaag atgcaatggg cgctaacatg
     3061 atcaactcga ttcttgagag tgtggcaaac aagttacggg agtggttccc tgaagaagag
     3121 attctattca gtattttaag taattttgct acagaatcat tggcttctgc ctgttgtgaa
     3181 atcccattcg aacgcttagg gagaaacaaa gaaatcggtg aacaaattgc caaaaaaatc
     3241 caacaggcag gggaatatgc gaagcttgac ccttaccgtg cagcaacgca caacaaaggg
     3301 atcatgaatg gtatagaagc tgtagtggca gctacaggaa atgatacacg tgcggttagc
     3361 gcttcgatcc acgcctacgc ggcaagaaat ggattatacc aaggattaac agactggcag
     3421 atcaaagggg ataaactagt tggaaaacta acggtgcctt tggctgttgc cacagtaggc
     3481 ggagcttcca atatcctgcc aaaagcaaaa gcatctttag ctatgttaga tattgattca
     3541 gccaaggaac tagcacaagt aattgctgca gtagggttag ctcaaaacct ggctgctttg
     3601 cgtgcattag ttacagaagg aatccaaaaa ggccacatgg gacttcaagc tcgttcatta
     3661 gcgatctcaa ttggggctat tggtgaggaa atcgagcaag ttgcgaaaaa attacgtgaa
     3721 gcagaaaaga tgaaccagca gacagctata caaatattgg aaaaaatccg ggaaaaataa
     3781 aaaagcaata tctttagaag aataaagcaa gatttacagt tattttattt actgcagatc
     3841 ttgctttttt ctctatcaga aaatatgttt attattagaa ttttttatag ttaaggttag
     3901 gcatcttaag aaatcaggca actccaagat atcttcaggt agaagagaag tcgcctaaca
     3961 ttgtaggatc ataggtgatt tgttggacag gtcaagctga tatttgagcc tgatgttaga
     4021 aggaatttct ttttacttgc tgtagaaatt attatatgac taaaaaggct gggattttaa
     4081 atcccagcct tttatgacaa atagaatttt tatcgatcag taatcgctgt cgctgcaaat
     4141 tgttcgatca aaggaatcaa agtcaatttg actttgtatt tccgttcgag tgctttttcg
     4201 ataacgaact cattttcagt agtcatagaa atagttgcta atttacgagg ttcatcagca
     4261 tttttttcat taataactac ttcatgttca ttttgttctg tgtaaaattt aggatactca
     4321 gcaacggctt ttgaaaaatc agcaaaactt aaagcggcat tcgtatcagt tgagcgtgta
     4381 ttttcagttg tgtccgcttt tggtttttcc gttttttctt ctggcacact gactgtttct
     4441 tctgtaactg gtgtcgcaac agcttgcgca gttggttcat cgtttttcaa tcgttgatca
     4501 ttccagtaag acacgatttc aataagaaga ttgtcacgga atcgttcgaa aatcccgttt
     4561 tctcctgaac gaattctctt taatacagta ttgaagttgt catcgattgg cacgttgatc
     4621 cgtgtatctt catttgtttt cccagctaat ccgatcgtta agatttgacg gtttttggga
     4681 tcaagcatga cttgtaacgg caaaccataa cgtcgcaagc tctcttcttc tagagaacgg
     4741 aataattgtc ctcttacacc atccaatgaa ggacggaatt gatttaataa tttttgtttc
     4801 tgttttttct tcatatgttc tctccttcgc tcataccctt tcgattataa gaaaaaaaca
     4861 gccaaatgaa aaggagtaat gcaaaaaaac ttgcgtatac gcaaattaca acagaaaaat
     4921 ttgcgtattt tcccgttttt tgaaagaaaa ataaaaaatg gaacggatta tgtatatata
     4981 cataatccgt tccattcttt ctattttttc ggtatcaagg caaaaacagg tagttcaggt
     5041 gaataaatga cttcatgttc gtgccagggt ttgaccatta gcgaatgatc gaaccgtttt
     5101 ttcagttctt cgatgatttg atcatgtgtc cagtttcttg caggaaaatg ttggattttc
     5161 tctgcatcga tcaaaaagac aaaatcggaa tgaaaagtct gggtaacttc ttccgagaga
     5221 gaattcaagc gttcgattgg atccataaaa tcacctttcg acttaaaatt gggtagtatc
     5281 tgggtctttt gctttgccga ctacaccatc taactgatcg atttgtttca tttcttcttc
     5341 tgttaattca aaatcaaata attcagtatt ttctttgatt ctgcttggtg tcactgactt
     5401 aggaagagga aggaattcgt gttgcagcga ccaacgcaaa gcaacttgtg caatcgtctt
     5461 gtcatgtgct tcagcgattt gtttcatttc aggcacatcg aagattttac cagtgcctaa
     5521 tggactatat gcttcgagga tcatatcatg tttttttgca tactcaacta cagttgtctg
     5581 taattcgcct ggtgcaagga aaacttgatt gaccatcggc ataatttttg ctgtttttgc
     5641 tagtgtatct agatggtggg gtaagaaatt gctcacaccg attgctttga ttttacctgc
     5701 ttcatacagt tcttcaaatg ctcgccatgt atctgcattt gcttgttccc aattatcacg
     5761 aaaagcaaca gggtttggcc agtggatcaa aaacagatct aaataatctg tttggagctt
     5821 cttcaatgat tcttcaaaag aagacatcac aagttcatag ctgtggtttg cattccataa
     5881 ttttgttgtc aagaatatct cttctctagg aattccactt tccttaatcg cttggccgac
     5941 gctttcttca ttcttgtatc cttgggcagt atcgatatgg cgataacctg cagccagcgc
     6001 ttctttaaca gaggaaacag cgacatcccc atcaggtgtt tgccatgtgc cgaatcctac
     6061 taccgggatt tcgtaaccat ttgatagacg ataagtgctt gttaatgaat tcatactttc
     6121 agctcctttt ttattcgttc aactataagt gtagcgcatt tttcgaaaaa aagaatccat
     6181 tttgtttcta ctcttgaaat ttttgtttaa aagaaaatga ttttgattct ggtatttttg
     6241 attttttttc cgtaaactaa gacagaaggg aatgagaaaa tggatatcta tttaaaaaaa
     6301 gcaattctgc atattattga cagagaaact ggttctccag tcttttcaca aaaagagctg
     6361 gatctgacaa aagaatatat tcgtgatttt cttcaaaaga agatccaaaa aatctcttct
     6421 gctcaaacaa aaactggaca actagcagaa gaaagcatga tcgttgaatc acttaaaatc
     6481 gctcaagaaa attttgtgga agcaagtgaa gcaatcgttc agcggtggtt cgatatctac
     6541 caagaaagtg aagaagcacc tagtgcagat gtatttgttg tcttgtatga attagataca
     6601 gtgatgtact tatctttatt gaaagtcaat tatcgtgagg cttacactca ctttgttgaa
     6661 gcagacgaag cagggatcga caaccgcttg attttgaatc gagcaattct aggggcgaaa
     6721 acacaaaaag cagatgaagc attcgctgtg aatttgactg attggaccta cgaattaatt
     6781 gaaaaaaaat atgagttttc tggcaaaaaa gagctttatt tttctggaag agtgatcgaa
     6841 agtgttcctg caccttcttt agaagaaaat gtaaaagtga tccaaaaagt agccaagcat
     6901 cttggaaaaa aatttgagac agaagaattt gatatcatgg ctgatttgaa agaagctgtc
     6961 tatgatacga tagaagaaaa aggtcggttg gatcatgaga tgattgcgga aaaagtcttc
     7021 aaggaaaaca tcacggcaaa aatggctttt aaagaagaag tacaagagca aggatttgtc
     7081 ccagaagcac ctcctgtaaa agaagtacaa gaaatttctg agaaaaaatt tggaaaacaa
     7141 aaattcaagc tatcaaacgg gatcgaactg atcgtaccag tagatgtata tcgcaaccca
     7201 gatttgattg aatttgtgaa taatccggat ggtacgatat ccgtgatgat caaaaatgtg
     7261 gatgaagtat tgaatcgttt ataaaaatga ctagaatatt aggtgttcaa gtaccagtga
     7321 tcagttaaat gaattgtgtc aagagaagga tcggaaaaga cgaatataac aaaaaaagag
     7381 cgtgagacaa aagtaaagaa cacttttgtc tcacgctcta aacacgtata aacggcggaa
     7441 gcagaagcaa ctccttctta tttcttggag ttaaacgctt ctgtcccagc ctctttttat
     7501 ctaataaaat tttaagacca tttactaatt atcgattttt tctgccgcat ttttaggcac
     7561 ttcagaagcc tttatttctt cccagcttaa tacaccttta cgaggattga ctttgagttt
     7621 aagatacgca ttcatcttta atggatgttc tctttcttcg cgaagcgtca cctgtttttt
     7681 ttcaccatct ttattataag ccgcttgctt gtaggtataa gtcgtgaatt tctctccgtt
     7741 atctgcttgg gtagctgaca tttcgccatt cgtcgtgatt tttgtataat aagcttcgcc
     7801 gccgtaaaaa taatgataag ctgcaaaact tccgccgaga aatatgccta aaacgacaag
     7861 tacgccaatt aattttttca tgtacttcac ctctttcctt attattaacg aaatagaaga
     7921 tttcgtctat tgaaagttgt tggcaacagc tgaattttaa acttttcctc attttgttct
     7981 tattgcttca aaagaaagtt aaggactatc ttgaagtttt tctcaaaaaa cacatttttt
     8041 tatttataaa aggtactttt ttcgcgtata agacagacga gtgctcttag ttgactttga
     8101 ccaatattga cgtataataa gcttagttcg aatcttacaa aaagtaagtc ggacgtattc
     8161 tttttgattt agtcaaaaaa agactataat aaaaaggtga gagctgaaat ttttcagtga
     8221 tctatattat ttaggaggtt tgtaaattat ggcaaaagaa cattatgaca gaagtaaacc
     8281 acacgttaac attggtacga ttggacacgt tgaccatggg aaaactactt taactgctgc
     8341 gatcactaca gtattaggca aaaaaggcct agcaaatcct caagactatg ctagtattga
     8401 tgcagctcca gaagaacgtg aacgtgggat tacgatcaat accgcacacg tagaatatga
     8461 aacagaaaaa cgccactacg cgcatattga tgcaccagga cacgcggact atgtgaaaaa
     8521 catgattaca ggggcagcac aaatggatgg tgcaatctta gttgtttctg caactgacgg
     8581 tccgatgcct caaacacgtg aacacatttt attgtcacgc caagttggtg taaaatacct
     8641 gattgttttc ttgaacaaag ttgatttagt cgatgatgaa gaattgatcg atttggtaga
     8701 aatggaagtt cgcgagttat tgagcgaata tggtttccca ggcgatgaca ctcctgtgat
     8761 caaaggttcc gcattaaaag cattgcaagg cgatccagat gctgaagctg ctattatgga
     8821 attgatggat acagtagatg aatatatccc aacaccagaa cgtgatacag ataaaccatt
     8881 actattgcca gtggaagacg tcttctcaat tacaggtcga ggaactgttg cctcaggtcg
     8941 tattgatcgt ggtgctgttc gtgtcggtga tgaggtagag atcgtaggga tcaaacctga
     9001 aacacaaaaa gcagttgtaa caggtgtaga aatgttccgt aaaacgttag attacgggga
     9061 agctggggat aacgtaggcg tgttgttacg ggggatccaa cgtgacgata tcgaacgtgg
     9121 acaagtactt gctaaaccag gttccattac tccacataca aaattcaaag cagaagtgta
     9181 cgtgttgaca aaagaagaag gtggacgtca tactccattc ttcaacaact atcgtccaca
     9241 gttctacttc cgcacaactg atgttacagg aacaatcaca ttgccagaag atacagaaat
     9301 ggtcatgcct ggtgacaacg tgacgattga tgttgacttg atccatccaa tcgctgttga
     9361 aaacggaacg acattctcaa ttcgtgaagg cggacggaca gtaggttcag gtatcgttac
     9421 agagatcgaa gcataagaaa cgcgactcga atattttggt aatgaaaaat gtttaaaata
     9481 aaagagcggt ccaaacagga tttctgtttg gaccgctctt tgttcttcaa ttgtataaga
     9541 agagattgta gaagcatgaa ctttcgtttt acttcagtct cttttattca tcatgagagt
     9601 gttcatctat cggtatgcag tttttaatac tttcttcgta tccatttttt caaaccactt
     9661 attctacctt gtgtctgctc aactggcgca ttatcaataa tcagcaaagc aatcataaca
     9721 tatgcaatcc aactatctct agaatataga gtataacgtg agacccagtt tgtagcgtac
     9781 ttgcttttat attctcttaa tccatggaaa gaataaaaat gagaaccaaa ctcgtaaacc
     9841 aagtatgcaa tcctttcctg taaaaaactc tttctggaag ttccgacatt tgaaagaggg
     9901 gccatcccta aattgaacca ttggatatgt tcttctttca tataggcaaa caaatgcaag
     9961 aacaagaaat ccatactgcc agaaggtgct tttttagaat catatcgcat cagatcaatc
    10021 gttcctactt tattgttcgt gtaagtaggc atgatcgttg caaaagcgac aatttcttct
    10081 tgggtatttt tgacaactgc aataggcgct ctttgtagat aatcttcaga gaaaaagcct
    10141 aaagaaaacc ctttttcctt tcgtgaaccc aaccaattat cagagatatt tttgaaagta
    10201 ctcatttgct cagtagaaaa tggtggttgt aagacatcaa atgtaaatcc ttctcgttcg
    10261 atccgattca agacagcacg agtgcccttc atttttttcc ctgaagttgt aaaactattc
    10321 aggtctacat aagcttcttc ccccatctta atgaaatcgt agccgaattc atgcaaaatc
    10381 ataacaatct cttcgcttgt ttcgtagaaa actggtaaat agcatagtcg atctgtttct
    10441 tcgataaaag cttcgattgc ttctggaaaa tcttctttct taccagaagg atcacccatt
    10501 acaatacatt tattattgaa gcaagcgaat tggagaagaa cagttggttc accgtctttt
    10561 tcataggcaa acatccgctt atcttttaag aagatcaatt gactatcgga gtttcctcca
    10621 taagttgtaa ggatcttcag ggccttttct tcattaaagg cttcgccaat ctgtttcttt
    10681 tctccttgaa ggaaatgaac aaatagaacg atcatgaaac taacagcgat gatggccaac
    10741 aatcccgaaa accaaatctt ttcagaagga aataagaaaa aggaaataaa atggtgacgc
    10801 cgatgaggga aatccggcag attgtatacc ccaataacga tgtaaagcaa ggacagtgca
    10861 ccaataatga ttccatcgat cgtacgccac tcccatgaat agatcaattg ttcccgaaat
    10921 aattcgtttt tagaagcaag aatgatcaga agcagaatag acaagaaaac caccggtgtg
    10981 aaactaaaat cacttaataa aacataaagt aaagctagaa caatcaagaa gattgtaggc
    11041 aaatatgctc gtttcacacg tgctgcgatc cctctgccca taatgattaa aagaaatccc
    11101 aatagtatac taggaaattg gataataaaa tgaaatttca atgggttcaa actatgaagc
    11161 cagcgaaact ctgtaaaagc ttgcgggata gtggcagaaa gtactagcat gattccagaa
    11221 aagtataata agacaaccac gattttatgc gcaatttctg ttgctaattg tttagggata
    11281 ccagaataac gctgatcaaa agtggaaccg atatttttga agaaaaagac gattccaatc
    11341 aaaaacggaa tgatgtagta gaacaggcga tagagcaaga tccacacaac gactatttct
    11401 cttggcacac caatagcgga caacccgata atcatcatta catcgaaact cccaatttct
    11461 cctggaatca tagacacgat tcctatcact gaagcagcaa tgaataatgg aattgtttgc
    11521 cacagatcaa tccggatttc cattaaataa ccaacagaaa ggaaactaaa caggacaccg
    11581 gtccattcca acaatgaaac tagcaatagc tgtccttgag ttttaagtga ggtaccacca
    11641 aggaatcctt tctttttgaa agtagtaaat aaaaagacga caggaaaata tagaccgccg
    11701 ccgatcaacc atatccaata ttgcttcaaa aaaggagcaa ctggcgtgaa gactacaagt
    11761 aaaaaagaaa gcagactgta aatagaaagt ccagccatca aaaacaagaa aactttcgat
    11821 aatgcttgga tcacgtttct gctctctttc ttttgtccat atagttcaga gcgaagcccc
    11881 acactaacaa acccgccgaa accggcaata ttgttgatcg tattgatcag ccagcttgtc
    11941 tcaaacaggt aacgaggatt ctgcttttgt tcaagcatcc gattcaaaat aatatcataa
    12001 ccaaccatag gtaacacaga gataagacca atcaatagca ttagtccgct tttccataca
    12061 ggtatagtgg caaacgtttc tcctaattgc tggatagata gagttttacc gatggacatc
    12121 aattcaccga caataatgac aatgacagaa atcaaaaaga ttgttttgaa cagtcctagg
    12181 tggtttttca gccattgtat ggtatttttt aacaaataaa tcaccttcta ttcctaatag
    12241 ttatgtttca gaaagaaatt gaagaacttc tcgattgaag cgttcaggat cttttctagc
    12301 taattcatgt ccttgttctt tcacaagcac aaaggaagct tttggtatag ttttagctat
    12361 atacaaagaa tgttttagct tgattacatc tttttttccc acgatgatca aagtggggga
    12421 gttgattttt tttaaatcat tttctgtaag cccgatgtca tgcagcaaaa gtttgataac
    12481 taaaagattt ttgcgaagac ttgggcgaaa taaagagagt aaccataccc atgcataatg
    12541 aatattactg atgactctgg cagaaaaacg aacgccttta acaagcgtgt ttccagaatt
    12601 taaaatcaat cgatgaactt ttttagggaa gctgctggca aataccagtg ctagatttgc
    12661 tccatcacta aatccaagaa aatctgcctg atcgattttt tccagcaaca tgatcgtatt
    12721 cagatcctcg gccattaact gaaaattcaa catagatgct tcgtttgtcg aacgaccatg
    12781 tcctcggcta tctactagat acacggtata gtagcgttcg agtacaggga cctgttctga
    12841 aaaaaagcgg ccgctgccgt cattgccatg cagtaaaaag agagggaagc cttgaccgct
    12901 cttttcatag taaatttcgc tcccatctgg catttttgca tatttctttt cttttttcac
    12961 ataatcacct actgtgtaaa gtataacgat tatatcagtc attgggcaat tgtttttact
    13021 gaaaaacaac gatcattttc gtaagatgtc aaaaaataca ggaaaattca ttttttctta
    13081 aggcgtacac agaaatcaag gaagaaatag ccaagaagat aaagaaatag taagataaaa
    13141 aaagatgtca aaataactag gtgatgaaat gaagcgaaaa cgattatcta ttatccgtat
    13201 tcttttttac atgatcattc tgacggttat tggtgcggtg ttgtttttat gaggaagaac
    13261 ttatcgttcg gtaaaacgag tggaaagcta tcaaatagaa ataacaacag ctgcaaaaaa
    13321 ttatcatatg gaaaactacg agaatttgat caaggcgata atattgaccg aatcaaaagg
    13381 aaaagggaca gatctcatgc agagctcgga aagtctgcat ggagaagctg agatcattga
    13441 taatccagct gacagtattg atcaaggtgt caaatatttg gcggaaatga tagtagaagc
    13501 aaagaaacaa ggatgcgatt tatggacagc tgtccaagca tataattttg ggaaagatta
    13561 tattgcctat gtaaaaaatc atggcggaga aaacacagta gaattagcag aagcgtattc
    13621 taaagatgtg ctgagcccac ttttaggaaa tgaggaaggg aaacaatacc gatattggcg
    13681 gatccagtcc ttgctttaca atggcggcta tctctatcat aatggaggta acatgtttta
    13741 tcctgatatt gtcaaaatga atgaaaaaat aatagagacc tatgaaaaag tctttaagta
    13801 aaatagcagg aaatgttcaa tcatgaataa aattaaatga aagaaggagt gcgcaaatag
    13861 tggaaaaaag gcaaaaaatg gaaaatcaag aagtggggta cacaaccaaa gactggatgt
    13921 tacggtttgt gaagggaatg tttatcggtt caggatttat cttgccagga gtcagcggtg
    13981 gtgcattagc agctgtcttt ggcatgtatg agcggctgat ttctttttta gcccacctta
    14041 caaaaaattt caaagaaaat gttttgtttt ttctgcctgt tggtctaggc ggagtgacag
    14101 gcatctttct actttctttt ttagtcagtt ttttattagg tgcgtatgaa acaacgatat
    14161 tatggttttt tgtcggatgt atcgctggaa ctgttcctgc gttatggaaa gaagcaggga
    14221 aaaaaggaag agatacttct gatgtgatca tcatggttgt tacttttgtt ctggcttatt
    14281 tgtttttatt atgcggcgct caattatttg acgggcaagt cgatcagaat tttggaacat
    14341 ggctgattgc cggcggattg attggtttgg gtatgatcgt cccaggattg agcccttcta
    14401 attttttggt gtatatggga ttgtataaag cgatggctga cggatttaaa gaagtacgac
    14461 ttgaagtgat tgtgccgatc ggaattggtg ggattctttg tgttcttgga ttgtcaaaaa
    14521 tcatggatta cattttctca aaggcatata gcaagctatt ccatttcatt ttaggtgtag
    14581 tttttgcttc aacgattatg atcattccta ccgattacgc gggattggga gttgctgggg
    14641 tactagcttg tcctgttctt tttatagccg gtacagcact aggttggtgg atgagccgtt
    14701 tggaggaaca atataagtaa agatagattg ataataaaaa cagcacacgg gtgattcaaa
    14761 caaaaaggtt ggcatctttt ttgtaaaatc attcgtgtgt ttttctataa attttataaa
    14821 aacaagttgt ttttaatctt ggaacacgct agacttaaac acgtgattat ttttgaaagt
    14881 tggcggaaaa tggagaaaaa gaaaatgaat ggacttacac agcaagaggt agttcagaaa
    14941 atggcttctg gccagcaaaa tgactaccag gaagatgcat caaaaagtac aaaacaaata
    15001 ttcagcgata atatattgac gttattcaat tttctgaact ttggtattgg gatttgtctg
    15061 ttatttgtag gtgcgtattc caacatggcg tatttagcaa ttattgtcgt caatatcgtt
    15121 attggaattt atcaagaaat acatgcaaga aatcttgtac ggaaattatc gattgtttcc
    15181 caatcagaag tactagtgat ccgggacggt aaagaacaat cgatttctac taaagagctg
    15241 gtattaggag atttggtaaa aatcggagca ggagaacaga taccagccga tttacaggtt
    15301 atatctggac gggcagaagc aaatgaagct cttttgacag gcgaatctga tttgattgtc
    15361 aaagaaactg gagctgaatt gctttctgga agctttttga caagcggttc tgtcatggct
    15421 gaagttatcc gagtaggtgc agataattat gctgtccgct tgactagcga ggcaaagact
    15481 cacaaagcta ttcgttctga acttgtagat tcgattcgca aagtatccaa attcactagt
    15541 ttcgtgatta ttcctttggg gatcatacta tttttggaag cactgttcat ccgcgatgct
    15601 tctatcaaaa tttctgttat tgcatctgca gcagctttgt taggcatgct tccaaagggt
    15661 ttggtattgc taataagtat tgcattaacg acaggggtaa ccaaactggc aaaaaaacga
    15721 atccttgttc aagacatgta ttcaattgaa acgctggccc atgtcgatac attatgcttg
    15781 gataaaacag ggaccattac tgaagggaaa atgagtatcc agcgggtaga agcactacat
    15841 ccgctgtacg agaaagccat cccagaaatt atggggacgt atctggcaga aagcagtgat
    15901 aacaacatca ctatgcgggc tatgagagat tattttgagc tttctaatcg gtatgatgtg
    15961 acaaacaaag tcgcattttc ttctgatcgc aaatggggcg cgatggaatt tccagaactg
    16021 ggagtcgtgt acgtaggagc gcctgaacgt ttggtggaag aagatcggct tccggacagt
    16081 ctaaaaacag cccaagcgaa tggctatcgc gtacttatgt tggggatcgc tccaaatcag
    16141 caattgaacg aagaaaaacc agcatttgtt gagccggttg ctctgtttga aatcgatgat
    16201 ccaatccgaa aaaacgctca agaaacacta gcttatcttc atgaggaagg tgtggatctg
    16261 aaagtgattt ctggagacaa tccttttact gtctccaata tcgctagacg tgcaggatta
    16321 ccgaattatg atgcgtatgt cgatctttct caaattactg aagaaatgga agtgcgagaa
    16381 gctgctcatc gctatacggt ttttggacga gtgagtccgc agcaaaagaa attgctggta
    16441 aatgaattga aagaaagcgg cagaacggtt gcaatgacag gcgacggtgt gaatgatgtt
    16501 ttggcgttaa gagaagcaga ttgcagcatt gctatggcag aaggtgatgg agcaacacgt
    16561 cagattgcga atctggtatt gttggactct gattttacta cattgccaga agtcctgttt
    16621 gaaggacgcc gagtggtcaa caatgtgacg aaagtctcag gcatcttttt tatcaaaacg
    16681 atttattcat ttattttatc gattatatgt gccgttacag caattggatt tccgtttatc
    16741 cctatccaaa tcacgctgat tgatttagcg attgaagggt atccttcctt ctttttatct
    16801 tttgaacaag ataaaagaaa aatcacttac cgttatttgc ctacagcact tattaatgct
    16861 ttaccaaatg ccttgcttgt agtagtgaat atcattgcgg tgtatctact tggaaaaaca
    16921 ttcgcatggg gaaatgctga aactactact ttgatgtatt acttgctgat tgggatcagt
    16981 tgcatggcag ttgtaaaagc ttgttatccc ttcaacccat tgcgcatctt cctagcagta
    17041 acgacgatcg tagggatata tgtagctgca atgctgttcc atggaatttt ggaagtaaac
    17101 ttagggttgg gagagagcgt aggatacttc tttgtattca tgctcgtaaa tattgttctt
    17161 cgagtattgt actggaaggc tgatattcaa aatcagttac tgaaaagagt agcaaattga
    17221 tgcttaatga gatagaaatg tgttatactt gaaaaagcaa tgaaatatga tacaatcaaa
    17281 catgtataat tgtgtccttt aaaatcgcac tgatttcagt tagtgcgatt ttttaccggt
    17341 tattctcctg aaatatcttc tgttaaaccc aaaagatatc aatagagcag ggggtgaaaa
    17401 aatggctcaa cggattcctc aagaggttat cgaaacagta cgcagtcaaa cgaatatcgt
    17461 tgaggtgatt ggacaatatg tccagttgaa aaagtctggt aaaaattact tggggctgtg
    17521 tccttttcat gaagaacgta caccgtcttt ttctgttgca gaagacaagc agatttttca
    17581 ttgctttggt tgtggtaaag gcggaaacgt gtttactttt cttcaagaac tagaaggtct
    17641 aagttttcca gaagcagtgg taaaagtggc agacttcgaa cagataccgt tagaagatca
    17701 atggcgtcag cgtactatag ctgtccaaaa tcctgaatct gctacgggga aactgattgc
    17761 tgctcatgaa aaagcagcgg aaatctatca tcacatgcta ttgcatacaa aagctggaca
    17821 gccagcttta gaatatttgc aaagtcgtgg gttgtcttta gaattaattg aagaattcaa
    17881 cataggtttt gcacctaatg agcgttcttt tcttcagcaa gtattcaaga atgaatcaat
    17941 ttcaaatgaa ttgttagaac gctctggatt gtttgtcacg catgatgatg gccgtatgtc
    18001 tgatcgtttt tatcaaagag tgatgtttcc acttcgtaat tctcaaggcc agacgatcgg
    18061 tttttcagga cgctggcttg cacctgaaaa agggcaggaa aaagatcagc ccaaatactt
    18121 gaatagtcca gaaacagaac tgttcaataa aagacaagtt ttatttaatc ttgataaagc
    18181 aagaagcgat atccggaaaa acggagaaat tttgttgttc gaagggttta tggatgtgat
    18241 tgctgcatgg caggctggta tcaaaactgg attagcttct atggggacaa gtctaacagc
    18301 tcaacagatc caacaaatgg aaaagctgac gaaagaattg gtcttttgct atgacggaga
    18361 caacgcaggt tttgaagcaa ccaatcgagg gattgaattg cttcggcaaa acagtcgatt
    18421 agcactttct gtcgtagtcg ttccagaaaa gcttgatcct gatgaatatc tccgtaagta
    18481 tggagaagca tctttccaag aattagtggc tcatgggcgc gaaacagtct ttacttttaa
    18541 atcacgttat ttaaaacaag ggaaaaattt agataatgaa aaagacaaga tcgaatacct
    18601 cgaagaatta ctagttgaac ttagccgagt tgtttcacca atcgaacaag atatgtatct
    18661 atctcaactg tcaacagagt ttcagatcgg cagagagacg atacaaaaac aactgcgcga
    18721 tcttcgaagg acaaatcagc aaaatcgtca agaacctcag gaaagtcaac acactcaggt
    18781 tacgagacaa gaaactgcac ggaaaaagag acctttgacg caagtagaaa aggcagagca
    18841 gattctatta tatcgtgcat ttaaagaagt ttctgtccgt aatattttaa aagaacgaga
    18901 atttcagttt attcatgatg catatgcaga agtttatttt ttattcgatg catatgtctc
    18961 acaacattat gaattcaacc ttgcagagtt tttagacttt ttgcaagatg aaaatttaag
    19021 gcggttggtg gtggaaatcg aatatttacc ggtggcagaa gaaagttcgc cacgagaaat
    19081 ccaagacttg ctagatgtga tcagcaaatc tggcttagct gatgaaataa cattaaaaaa
    19141 acaaatgcag caagaagcca gacgaactgg aaacaaacag cttgagcttg aattgacgat
    19201 tgaaatcatc aatttaacga aacagttaaa acaagccaaa tagctactca aggggggcct
    19261 tcttttatgg caaatggaac agataaaaaa acgtatgaag cagcagtagc agctttcatc
    19321 aaagaaaaca aacccaaacg acaagttgta tatgatgatt tatccaatca gcttgctaca
    19381 ccattcactt tgaatgcaga tgaaatggac aaattgatcc aaaagattga agacgcaggt
    19441 atcagtgtgg tggatgaaaa tggtgatcca tcaattcaca gcttgaagag tgctgagaaa
    19501 aaagctgaac aagcaaaaac agaagatctt tctgcgccaa ccggtgtaaa aatcaatgac
    19561 ccggttcgta tgtatctgaa agaaattggc cgtgtctccc ttttaactgc ggaagaagaa
    19621 gtagcgttag cattgaaaat cgaagaaggc gaccaagaag cgaaacagcg tctggcagaa
    19681 gcaaaccttc gtttagtagt aagtatcgct aaacgttatg taggacgagg catgcagttc
    19741 cttgacttga tccaagaagg aaacatggga ctgatgaagg cagttgaaaa atttgactac
    19801 cgcaaaggat ttaagttttc aacctatgct acatggtgga ttcgtcaagc catcactcga
    19861 gcaatcgctg accaagcacg tacgatccgt attccagttc atatggttga aacaattaat
    19921 aaattgatac gtatccaacg tcaattgctg caagaccttg gaagagaacc tacgccagaa
    19981 gaaatcggtg cggaaatgga tcttccaaca gaaaaagtcc gagaaatctt gaaaatcgcg
    20041 caagaacctg tttcccttga aacgccaatc ggtgaagaag atgactctca cttaggtgat
    20101 ttcatagaag accaggaagc aacaagtcca gcagaacacg cagcttatga gttactaaaa
    20161 gaacaattgg aagatgtact agatacattg actgatcggg aagagaatgt ccttcgctta
    20221 cgttttggtt tagatgatgg ccggacgaga acattggaag aagtagggaa agtatttggt
    20281 gttactcggg aacgtattcg tcagatcgag gctaaagcat taagaaaatt gcgccatcct
    20341 tctcgttcaa aacaattaaa agatttctta gaataagcgt ggactattga ccagaagaga
    20401 aatcagtaaa actgtttggt ttattttaaa agaaaaaaag cttgagacta cgcagtctca
    20461 agcttttttt tctataggaa ggaaattcat tcattatttt taagtaaatc ctaaaaataa
    20521 gctattggcg tgacgtatag aatatgatag attaaatggt agcgtaaacc tagtaaagga
    20581 gccgatcgct aagtgatgaa aaaatgtcca aattgcggat cgaaaaaaat ggagcagaat
    20641 actatctgtc cagactgcgg tcatcaaata actgcagaag atcagcaaat aaaagaatca
    20701 attgaagaaa caaatacaga tgcagctatg caggataaat caactgaaga tgcagtcttt
    20761 cctgatacag aattaaacga tccaatcgaa tggtcggaat tgaaagattt acctctagaa
    20821 tctgtaatgg aattattcga aaaagcagaa ccagaagacg aagaaccacc tgctcacgat
    20881 caagtaacta atatagagaa aaatgaacag aagaaagcaa ctgttcatga gacttcggaa
    20941 gaatcaggaa agaatcaaga agaaacgctt gaatcaaaac aaaaaagaag agtagctgaa
    21001 ctgaaagaaa tcgtcgataa tgaggaagaa aattcgatac tatccgctta tatcaaagca
    21061 catcgtgaag atacaaaaga agagcacgcg aaagagctgc tcaaaatgat cagtgaaaaa
    21121 attgcacagg aaaacaaagt agagccagaa aaggaacaag aaactgaaaa agactctgat
    21181 agagaaaacg ccacaggtaa agaaacagta aaaataaaag acgtaaaaac ggatatcaca
    21241 gaaaaaactg aagtatttac tgaaaaacaa ttagaaaagg aaaaaacgaa taaactgccg
    21301 gaagcagaga aaaaagaaat actcgaaaac acagacaaaa agtcaacaga gcccgatgaa
    21361 ataaatagtg aatccgatgc tgaaaatcaa tctgtaagca aagagggtac tgatcttgaa
    21421 gcaaaaatag aaaaagaaga gaaccagcct aagctgtttg aagcagaagc gaaatcaata
    21481 acaatcgtgc cagatactga aaaaacacat caagaatcaa acttagaaaa aacagatcca
    21541 gataaagcgc aaaagaaaaa atcaaaaaaa gtaccttatc tcgttttagc agcagtactt
    21601 ctactcggtg gaggaagctg ggcttattat gaccatcatc aaaaagtaga agccgaagta
    21661 gcagcagaag caaaaagaca gcaacaaatg gaagaacttc atgagaaact tgctgccttt
    21721 tatacagatg atcaccatca atttattcgt gcagatatga ttcatcaaga tttatcaaaa
    21781 ctaaaaaatg atttatccag tcttaagaat gaaaaaggat attcagagtt agaaacaact
    21841 taccaagata tccaatctaa gatccgagat atccaaaaag tgaatgagtg gttcatagca
    21901 cctgccatcg tcgatgatca tctagcagat gacatcaagt tgaaagcaga taaagagatc
    21961 caagcaatcg aaacggataa cacagatttt ggaaaattga ttcaacaagc acaaacagaa
    22021 gctaaaaatc aatatgctga attgcaaaca gctaaagaaa aaacagctat agtctttaaa
    22081 gacggaaaag tagtggattc agcaacacgc gaccaataca agacggcaaa agaagcagta
    22141 gaaaaagtga aaaatacaga tctatctgct cagttgaatg aacaattgaa acaagtagat
    22201 aagatgctca ctgataaaga gaaaaaagaa gaagaggaaa agaaaaaagc agaagaagcg
    22261 aaaaaagcag ctgaagaaca agcaaaacaa gccgctcaag cagcagctgc agctcaagcc
    22321 gctcaacaag ctgcggtcgc acaagcagca cagcaagaag cggcatcctc gaccccttcc
    22381 tcttcaaact cagcgaatca gccaatcaaa tctgccagag caagtgatat tgccgatgca
    22441 tctaatccag catggacgtg ggcaccaggt gtccaaagca aagtgattgc tacatgtatc
    22501 caacgtggat atatcgttga aggaggttac agattagaaa aagccagaat cgaaaatggg
    22561 gaaggctatt ataatctata tgccacttca acaaaatctg cattgatgaa aggcattgga
    22621 gaaagtgctt tgccatttta catcgtaacc atcaactgta aaactggatg gttcggagga
    22681 aatgggagca attaatttta ggcaaatgaa agaggctgtg agcctgccgt ttaacaccgt
    22741 ttattcgaaa aaggggagta tgacacaact gttgtcatac tccttttttc acctttccaa
    22801 accaaggctc agctattcaa gtagataaca ttatgttatg atagcaaaga aatgtattaa
    22861 gaaaaccttt cactatagag ggagaaaaaa atgaacgatt tattagcaac tatatttaca
    22921 ggtattatta ttgatgaaaa tgaaaaaaac tactttgtcc aaaaaaacgg acaaaccttt
    22981 ggcttaagca aatcagaagg tgaacacaaa atcggtgaag cagttgaagg attcggttac
    23041 caaaatcaaa aacatgaaaa tcggatcact acacaaattc ctaaaagccg tatcggtcat
    23101 tacgcttttg gaatcgttac tgctacacga aaagatttag gtgcgtttgt cgatatcggt
    23161 cttccagata aagactttgt cgtatctctt gatgaattgc cgacaatgac agagctatgg
    23221 cctaaaaaag gggatcgctt gatgatttcc cttagagttg atgcaaaaga ccgtatttgg
    23281 ggcagtttag cagatgaaaa aatattcaaa tctttaagca aatatggaac agaagaaatg
    23341 aaaaacaaag atgtgatagg aacagcatac cgcttgaaat taacaggtac gtacgtttta
    23401 acggaagatt tctatcttgg gtttatccat ccttctgaac gatataatga acctcgtcta
    23461 ggcgagcaga taaaaggtcg agtgattggt gtccgaccag acggtgtact gaatctttca
    23521 ttgaagccaa gagcatatga agctatatca gatgatgcac aaatgatttt aaccatttta
    23581 gaacgttctg cagatcagca aatcaacttt acagataaat cggatccaga agaaatcatg
    23641 aaagcttttg gtatcagcaa aggagcattt aagcgcgcat taggtaatct tttgaaacaa
    23701 ggattgatta cacaagaaaa cggtgtgaca aaactagtca aaaaatctaa ttgagaatcc
    23761 cttgcatgtt tttcttaact agcttataat aattttaatt aacatagtgt tatatctttt
    23821 tttatttgta attattataa attgttgggg ttgagtaaat ggtcatgaat gctatttcgg
    23881 cgatgaaaaa aacaaaacag cagctacatg aagcggggtt taagctgaca ccgcaaagag
    23941 aagctactct gcttgtgctt ttggaaaatg agaaagagca tttatctgca gaagaaatct
    24001 tctttttagt gaaaaaaaag agtccggaaa ttggacttgc tactgtttat cgaacccttg
    24061 aaatcttgac agaattgaaa attgtcgata aagtaagttt taatgatgga ttagcccgtt
    24121 atgatttgag aaaagaaggc gccaaacatt tccatcatca tttactatgc ttgaactgcg
    24181 gcacgatcga ggaagtagaa gaggatcttt tacttgaagt ggagcaagta gtagaagaac
    24241 gttatcattt tttagtaaaa gatcatcggc taacgttcca tggaatctgt caggaatgct
    24301 atgaaaaaga aa
//
LOCUS       DACDIE010000039        23993 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.039, whole genome shotgun sequence.
ACCESSION   DACDIE010000039 DACDIE010000000
VERSION     DACDIE010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.039"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..619)
                     /locus_tag="IV250_001719"
     CDS             complement(<1..619)
                     /locus_tag="IV250_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein"
                     /protein_id="HAP8769003.1"
                     /translation="MKTLFDYILTLTGGSVEFMAEKRMLSKTFLNNASFMKMSDSAKL
                     LYVYLNVNADDDGIVEAYPYVRMLGTPDDDVKILLAKEFLYMLNEDLLVFLPHWLQNQ
                     TLRTTRYTPSIYRFKLLNSNPDFFTRLKQISIEKILKAGQTEMLRNDLIEKWYPVGLP
                     KDDHGHAQVRLGQVSSDQSRSGKVSLGEIREDQSINLSDDMEVEIM"
     gene            complement(809..1093)
                     /locus_tag="IV250_001720"
     CDS             complement(809..1093)
                     /locus_tag="IV250_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8769004.1"
                     /translation="MDVSSVQVILPPDLDQSLRKYFYQVAKEAVDQVRIETSLSKEIF
                     RLGEACEYLSCSRSTLKKFINQRGLKVSIIGDCQFIRKEELARFIKEYEI"
     gene            complement(1106..1399)
                     /locus_tag="IV250_001721"
     CDS             complement(1106..1399)
                     /locus_tag="IV250_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8769005.1"
                     /translation="MTDNEINAILSLVKTKKDEVVRLDSPVRKYRRFCGYRQKELAEL
                     LEISELTYRNKEKGRVPFKDYEIERIVKLFHKDGIHLDIVDIFFSDLKTKKDE"
     gene            1468..2232
                     /locus_tag="IV250_001722"
     CDS             1468..2232
                     /locus_tag="IV250_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8769006.1"
                     /translation="MNLNKTSVGNRIRLVRKELGLSMKEFGKKITPTAADSIVSRWER
                     GISLPNNDRLTQIAKIAHKDINWLLWGTFKEFISSLLDKEGYTNFLNDFPETVDTIYN
                     DLMPISENLQFPDYENSILRLFNKIYLPIFNDYCLDIIELDLEDFINKRHSISSDKFK
                     NRFLSSFHEGVSNSGYKYGDTDLIYKYGIALLEKFDDSYKLNNPYESIDAYFSAMTVN
                     TEDTEKLIYTICKSLGIPFQKGSNTAQILSKNHNNF"
     gene            2283..3428
                     /locus_tag="IV250_001723"
     CDS             2283..3428
                     /locus_tag="IV250_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8769007.1"
                     /translation="MATIKQYEKKDGTKAWLFQTYLGISPLTGKEVRTTRRNFKTKKE
                     AQIELNRLLVDFEENGLKKETNETFKEVYDLWYESYKTTVKEVTQLKIEIKFNKWILP
                     KYGQFRIKEITTKHVQKILNQWAKTTDQYKVLHSTASRIFKYAMTLGIITKNPCELVI
                     MPNRNVEKKKDTVKIYSRDQLESLFQYLESKRSTYRADYDKTLLRFLFFSGCRISEAL
                     ALDWSDIDFDSNTVTINKTLSQTKYGYKISSPKTRNSYGSLSLDNKTINWLKKWQINQ
                     KRYMLTLGITDPTMIFCGIYKEIITKHAIYSRMITICKNSGIPFLGNHVTRHTHASML
                     LESGVSMKEVQERLRHSSIKLTMDTYAHLTKETKEKTVEKLAEHLNF"
     gene            complement(3513..3818)
                     /locus_tag="IV250_001724"
     CDS             complement(3513..3818)
                     /locus_tag="IV250_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAP8769008.1"
                     /translation="MTEHNHSHEHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(3844..4275)
                     /gene="ruvX"
                     /locus_tag="IV250_001725"
     CDS             complement(3844..4275)
                     /gene="ruvX"
                     /locus_tag="IV250_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAP8769009.1"
                     /translation="MRVMGLDVGSKTVGVAVSDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(4272..4538)
                     /locus_tag="IV250_001726"
     CDS             complement(4272..4538)
                     /locus_tag="IV250_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAP8769010.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(4792..5517)
                     /locus_tag="IV250_001727"
     CDS             complement(4792..5517)
                     /locus_tag="IV250_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAP8769011.1"
                     /translation="MSKKTTKAIVGIVAAVIAGALGISTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(5883..5953)
                     /locus_tag="IV250_001728"
     tRNA            complement(5883..5953)
                     /locus_tag="IV250_001728"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5919..5921),aa:Gly,seq:ccc)
     gene            6070..6822
                     /locus_tag="IV250_001729"
     CDS             6070..6822
                     /locus_tag="IV250_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAP8769012.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            6838..7317
                     /locus_tag="IV250_001730"
     CDS             6838..7317
                     /locus_tag="IV250_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAP8769013.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(7568..9148)
                     /locus_tag="IV250_001731"
     CDS             complement(7568..9148)
                     /locus_tag="IV250_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAP8769014.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(9141..9884)
                     /locus_tag="IV250_001732"
     CDS             complement(9141..9884)
                     /locus_tag="IV250_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8769015.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(10060..10836)
                     /locus_tag="IV250_001733"
     CDS             complement(10060..10836)
                     /locus_tag="IV250_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8769016.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(11084..11920)
                     /locus_tag="IV250_001734"
     CDS             complement(11084..11920)
                     /locus_tag="IV250_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HAP8769017.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(11931..12860)
                     /locus_tag="IV250_001735"
     CDS             complement(11931..12860)
                     /locus_tag="IV250_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAP8769018.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            13052..13405
                     /locus_tag="IV250_001736"
     CDS             13052..13405
                     /locus_tag="IV250_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP8769019.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(13479..14135)
                     /locus_tag="IV250_001737"
     CDS             complement(13479..14135)
                     /locus_tag="IV250_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP8769020.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            14361..16295
                     /locus_tag="IV250_001738"
     CDS             14361..16295
                     /locus_tag="IV250_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP8769021.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     EIEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(16381..17604)
                     /locus_tag="IV250_001739"
     CDS             complement(16381..17604)
                     /locus_tag="IV250_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HAP8769022.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(17598..19496)
                     /locus_tag="IV250_001740"
     CDS             complement(17598..19496)
                     /locus_tag="IV250_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAP8769023.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(19490..20608)
                     /locus_tag="IV250_001741"
     CDS             complement(19490..20608)
                     /locus_tag="IV250_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP8769024.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(20691..21524)
                     /locus_tag="IV250_001742"
     CDS             complement(20691..21524)
                     /locus_tag="IV250_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8769025.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVSATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(21517..22434)
                     /locus_tag="IV250_001743"
     CDS             complement(21517..22434)
                     /locus_tag="IV250_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8769026.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(22661..23785)
                     /locus_tag="IV250_001744"
     CDS             complement(22661..23785)
                     /locus_tag="IV250_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP8769027.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
BASE COUNT         7535 a         4739 c         3916 g         7803 t
ORIGIN      
        1 tcataatttc gacttccatg tcatccgata gattgataga ttgatcttcc ctaatctcac
       61 ctaaactaac cttacctgac ctactctgat ctgaactaac ctgacctaat ctaacctggg
      121 catgcccatg gtcgtccttt ggtagaccaa cgggatacca tttttcaatc aaatcatttc
      181 gaagcatttc cgtttgacct gcttttaaaa ttttttcaat actaatttgt tttaatcttg
      241 taaaaaagtc tggattacta tttaataatt taaatcgata tatagagggt gtatatcttg
      301 tagttcttaa cgtttgattt tgcagccaat ggggaagaaa tactaataaa tcttcattca
      361 gcatatagag aaattcttta gctaaaagaa ttttcacgtc atcatcaggg gttccaagca
      421 tccgtacata tggatatgct tcgacaatcc catcatcgtc tgcgtttaca ttcagatata
      481 catataataa ttttgctgaa tccgacatct tcataaaact cgcattattt aaaaaagttt
      541 ttgacaacat acgtttttct gccataaatt ccacacttcc tcctgtcaat gttaaaatgt
      601 agtcaaataa agttttcaca gaactttatt attgagtgtt cttccggtct gcaaactgta
      661 aggagcactc tttttttgta tattcacctt ttattaaatc aaatagaaaa taaatcttga
      721 tttcttttaa actcaacttt gcttcttcat catcgttcaa acaaacactt cctttttttc
      781 atatcaaaag cacaccacgc aacttttttt atatttcata ttctttgata aatcttgcta
      841 actcttcttt tctgataaat tgacagtctc cgatgatact cacctttaaa ccacgctgat
      901 taatgaattt ttttagtgta cttctactac aactcaaata ttcacacgct tctccaagcc
      961 ggaaaatttc tttgcttaaa gatgtctcaa tacgaacttg atcaacagct tccttagcaa
     1021 cttgataaaa atatttacgt aatgattgat caagatctgg cggtagaata acttgtacac
     1081 tcgatacatc catgatgccc actcctcatt cgtctttttt cgtcttcaaa tcactaaaaa
     1141 aaatatccac aatatctaaa tgaattccat ctttatgaaa tagctttacg attctttcta
     1201 tttcataatc tttaaacggc acccttcctt tctctttgtt acgatacgtt agttccgata
     1261 tttctaataa ctccgcaagt tctttttgtc tatatccaca gaacctgcgg tattttctaa
     1321 ctggagagtc caaccttacc acctcgtctt ttttcgtctt taccaacgat agaatagcat
     1381 ttatttcatt gtccgtcaat acttacccgt cgtttttcgt agattccatt ccgtttttgt
     1441 gttacattac tgtcggaggg atataatatg aaccttaata agacatcagt aggaaaccgt
     1501 attcgtttag ttcgtaaaga actcgggctt tccatgaaag agttcggaaa aaagataact
     1561 cctaccgccg cagatagtat tgtttcgcgt tgggagcgag ggattagctt acctaataac
     1621 gacaggctaa ctcaaattgc taaaattgct cataaagata ttaactggtt attatgggga
     1681 acttttaaag aatttatttc atcattgtta gataaagaag gatacacaaa ttttttgaat
     1741 gattttcctg aaacagtaga tactatttat aatgatttaa tgcctatttc ggaaaattta
     1801 cagtttcctg attacgaaaa ttctatttta cgactattca ataagatata cttacctata
     1861 ttcaatgatt actgtctaga cattattgaa ctggatttgg aggactttat aaataagaga
     1921 cattccatct cctctgacaa gtttaaaaat aggtttttat cctcctttca tgaaggagta
     1981 tctaacagcg gatataaata cggcgatacg gatttaattt ataaatatgg aattgcatta
     2041 cttgagaaat ttgacgattc ttataaatta aataatccat atgagtcaat agatgcgtat
     2101 ttttctgcca tgactgtaaa cactgaagat accgaaaaac ttatatatac catttgtaaa
     2161 tcgcttggca ttccattcca aaaaggaagc aataccgcac agattctttc aaaaaaccac
     2221 aataattttt aattcatttt ttctatcaaa agcacgccac gcaactgata ggaggaaaaa
     2281 caatggcaac aattaagcaa tatgaaaaga aagacggaac caaagcttgg ctatttcaga
     2341 cctaccttgg aatcagtcca ttaactggta aagaagtccg tactacacgt agaaatttta
     2401 aaacaaaaaa agaagctcaa atcgagctta atagactact tgtagacttt gaagagaacg
     2461 gacttaaaaa ggaaacaaac gaaactttca aagaagtgta tgatctctgg tatgagagct
     2521 ataaaactac tgtaaaggaa gtcactcaat taaaaattga gattaagttt aataaatgga
     2581 ttcttccaaa atatgggcag tttagaatta aagaaattac tacaaaacat gttcagaaga
     2641 tattgaatca gtgggctaaa acaacagatc aatataaagt cctacattct actgcaagcc
     2701 gtattttcaa atatgcaatg actttaggga taattactaa aaacccctgt gaacttgtaa
     2761 tcatgcccaa tagaaacgtt gaaaaaaaga aagatactgt caaaatttat tcacgggatc
     2821 aactagaaag tctcttccaa tacttggaat caaaaagaag cacctataga gccgactacg
     2881 ataaaacgct acttcgcttt ctatttttta gtggttgtcg aattagcgaa gctttagctt
     2941 tagattggtc agatattgac tttgattcaa acaccgtcac tataaacaaa accttaagcc
     3001 aaacaaaata tgggtacaaa atttcaagtc caaaaacgag aaatagctat ggttctctat
     3061 ctttagacaa taaaaccatt aattggctaa agaaatggca aatcaatcaa aaaagatata
     3121 tgttaaccct tggtattact gatccaacaa tgattttttg tggaatctac aaggaaatca
     3181 ttactaaaca cgctatttat tctcgtatga taacaatctg caagaattct ggtattccat
     3241 ttctaggtaa ccatgtaacc agacacactc atgctagtat gctgttagag tcaggagtct
     3301 ccatgaagga agttcaagaa cgccttagac actcatcaat caaattaaca atggatacct
     3361 atgctcatct aactaaagaa accaaagaaa aaaccgttga aaagcttgca gagcacctaa
     3421 atttctagcg gaacgtattc aatctcgtat tcaattcaaa atacaaaaaa gctgaaccct
     3481 tatctaccaa gggttcagct accatttaaa gcttattctt cttcttccgc catgaatgta
     3541 ttgaatactt cttcgatcat atcccattct gcgtctgttt cgatttgttc taactcgcct
     3601 tctgttccat cttcgttttc gatgtatgca taagcttgta attctacatc ttcatcttct
     3661 gaaacaccag ctggatataa aagaacatag ttacgtccaa attcttcttg tccgtcaact
     3721 gttagcaaga tttcgtataa tgtttcattt ccttggtcat ccaccaatgt gatgtgttcg
     3781 tgtccttcgt gttcgtggga atggttatgt tctgtcataa gattacctct tttcttttta
     3841 ttattaaaat aattgcttac ctgagccgtc taaataattt tgtaaaatca tcacagctgc
     3901 taatttatcg atgacttttt ttcgtttcgc tcttgaagta tttgcctgct ctactagcat
     3961 acgttctgcc tgaacagtgg ttagtcgttc atcttgataa tcaaccggca aggagaatag
     4021 ttctttgatc ttctctccat atgacatgga agcttctgcc cttggtccga tggaattgtt
     4081 catattctta ggtaatccaa ctacaaaacg gtctacttcg tactgcgcca ctaattcctt
     4141 caaccgatca aaaccaaact cttcttctgc ctcattgatt cgaatgattt ctactccttg
     4201 agctgtccag ccaaagggat cgctcacagc aacgcctact gtcttagaac cgacatccaa
     4261 gcccattact ctcataaatt cactccatga tttgagaggt agtctttgac aattacttcc
     4321 atgatttcat cgcgttcgtg acggcgaatc aaatttcgtg catcacgata acgaggaatg
     4381 taggccgggt ctccagaaag caagtagccg acaatctgat tgattggatt gtagcctttt
     4441 tcttccaaag cccgataaac aatcgctaat gtttcgctca cttctttttt gcgactatcg
     4501 tcaaaaccaa aacgtacggt ttcatctgta aaacccatga ttctacacct ctttcccgca
     4561 aggtaattcc agcttacctt ctacaccctt attttactcg aagaaaggga aaactacaaa
     4621 caaatattcc aaacttctga ataaattaag aaacacgatt tgtctgattt ttcctttttt
     4681 ctgtggcttt ttcaaaagcc ctctttccat aaagtatacg ctatatttct ttgaaagtat
     4741 gtttttacac aattttttca taaagttcat aaaaaattcg aattgctgct actaattttt
     4801 cgttttcgtt gcttgccctg tctcataatt gatggtgtag ccatcttgaa cattgtagat
     4861 aaagacattg aattcaattt ttttgtcttc aatgctttct gcttccaact gcacacctct
     4921 tgctaccagt tcatttccct cgaaatgagg agtgacacga tagcgtacat gatgatttgt
     4981 ttccttgata tatgccgcaa tatcattttc gtgggcaagc atgtacggag cattcaacga
     5041 acgtgtccct gtcattaagt ttttaatgtt attattttga ccagtcaact ggaaaccaat
     5101 caaatggctg cggttgtata accattgtcc gtcacttaat ttcttttgtt tccaacctgt
     5161 cggatcaata tacaaaggtt ctctcttttc agtaggaaac aagtcttttc ccaacatcgc
     5221 attggcttgt cctactctgt tcaatttgtc tatatttgaa tagctttccc atgtcccttt
     5281 ggaaagacta agatcctctt ttgtaaactc cggctgattc ccattgattt ctatttcttg
     5341 atgttttcca tcatattctg gcacatccgc tacttgagaa gcttgcggtg tggaatcaaa
     5401 aatggttgta atttgttgga agatatctga atctttttgt gtggtggaaa taccgagtgc
     5461 ccctgcaatc acagcagcga cgatccccac gatcgccttg gttgtttttt tgctcatttc
     5521 tttactcctt tactaacagt tccctaaatc ttaacatatg ttcccctata aataaatact
     5581 ggaaagaaat agaaacagta aaaaaataaa accagttttt agacggctta agaatacaaa
     5641 aaaagagacg gattcatcgt ctctactcaa tcacaaaaca ctatttacaa ggtatttatt
     5701 atcattcaaa aaatttttct taatactcaa aactaaacgg cttctgtacg acctcttggt
     5761 aaacagtgtt caaaagcttg catctgcggt aataagtcca accaagtcaa aaacataaga
     5821 agcttttttg cttgactatt cttattactt gatgcagggc agctttttca cagcctcttt
     5881 taagcgggtg acgggaatcg aacccgcgac atcagcttgg gaagctggga ttttaccact
     5941 aaactacacc cgcgatacaa aaacagtata cgcctctttc actccttcct caagtttttt
     6001 tattctttac gcttccgata tgttttgtag taaaataaaa ctaatcaatc aattattgga
     6061 gaagatgcta tgacgggaaa agaattagat ctgttgctgg acacttgttt actcgctggg
     6121 aaaatcatga tggaaagcaa tgcggagatg tatcgggtag aagatacgat gagtcgaatt
     6181 gctttagctt ctggaaattt ccgtttggtc agctatgtca ctcaaaccgg tctctttatt
     6241 ggtcttgatc gtacctcgac catccgaatg gaacaaatta ccaaccgttc gattaattta
     6301 gaaaaagtag taaaagtaaa tgatctttca cgtaaatatg ttgcttcaga gctaacatta
     6361 gaagaacttt actgcgaact gcaagcaatc gaccgagacc gttctttctt tcctgtctgg
     6421 ttgcaaatcg ttagcgcggc agtgatcagt ggaacaatta tggtgttatt tggcggaaag
     6481 atgaccgatt tacctgtgac tttagtcatt ggaggattgg gatacgcagc ttatctatat
     6541 agtttgaaat tttttagaat caaattttta tcggaatttt tatcttcttt attgattggc
     6601 atagccgcta tctacagcgt acgttttggc ttaggaagca atcaggatct aatcattatc
     6661 ggatgtgtga tgcctttagt tcctggagtt caaattacga acgctatccg tgatttgcta
     6721 gctggacatt atgtctcagg agtttctcgc ggaacagaag cgatgatgac tgctacgatg
     6781 atcggttttg cgattgcttt tattttccag cttttttatt aggaaaggaa cacaagtatg
     6841 gggacgatat ttattcagtt ttcttttagt tttttagcga ctgcggcatt tgccgttatt
     6901 acaaatgtac ctaggcgttc attgatttgt tgtggtcttt ctggtgcttt tggctggatg
     6961 atctactgga tctgtgttca aataggcgga tctgctgctt ttggttcatt gcttggttct
     7021 ttaggagtag cattcatcag cgatcttttc tctaaacgtt tgaaaatgcc cgtaacgatt
     7081 ttcaatattc ccgggatggt tcctctagtc cctggtggtt tagcttatca agcggttcga
     7141 aatctcgtta ctgaatccta tcgagaagcg gctcattatg cggtccaagc aattatgatt
     7201 gctggagcaa tcgctttagg tcttgtatta tcagaagtct ttaaccataa catccgtaat
     7261 ttcaaagtaa agcgtgacgc aatcatgatg aaacgaaaaa aacggctttt taaataaacg
     7321 aagtgtaaca aagaaaaggt tgtgaaagag acgttcagcc ttgagcgatg acaaaatatc
     7381 tcacaaacat tgcgacaagt aaccgcagga gcaataagaa gtaattatga gtattaaaaa
     7441 aagttttgta aaaatagctt ctcacatttt tacataaaat tgacttaata ctgagaaact
     7501 gacttttgaa caccctctat ctgtaaaaaa ggggctgcga catgattttt gtcacggccc
     7561 ctcttcttta ttcaaatttt ttccaaaacg ttcgttgata ataaacgatc catagagcgg
     7621 aaagtattag aactaaaccg atcacaggaa tattcactaa ccaaaatgga taaaacaaag
     7681 ccaacattcc gtataagacg ataagaataa tactgatgat caatgaaccc atcatagttg
     7741 cgactaaacc caaacttcct gaacctctgt taaataattg gttaatattg gtccagttca
     7801 gcagcagcaa ccgataatct ctagcgaaat aacgtagtga caataagtag cagccgaata
     7861 aagaaccaaa tagcaagcta gggataaaaa acaaaggcag ctgaaaagac agtccgctta
     7921 ataatgcgat cccaccagtt aaaaaactct gcaatatcca tccgaatctg aacttttctt
     7981 ttaaatattg gttcatagag attggcaaag aacggataaa cagaaaattt tcttgatcca
     8041 aagaaataag attgctgata aaagaagtct gattaactgt taagaaagcc aatgcgatcc
     8101 ctaccaggaa aacaacaccg attaacttat tatctaaatc agttaatttt atttcgcctc
     8161 ctagggcaaa agcaagaata aaaatgattg gagttaacaa ggaagaagaa agaacttgca
     8221 taatcaaatt aggttctttt actaattgag cattgtaact aaataaaagc tgatgcacat
     8281 tttggttctt tttatgggtt ctttttattt tgaactcact gtttgaatca gtcagttgct
     8341 ctgtaagctt tggtactatc agctgtttta cagcaaggaa aaatcccaga gccgtgatag
     8401 ctagtattcc ccaacccaat aaaccttgtt gagaaagtgg ctgactggca gcatcataga
     8461 atggcaataa tatcgaaatc ggatgccgat ctgttaactc attttcaata gtccccgttt
     8521 gatgattcat ccacatgatt ccagcaaatg cgatcagcat cgagctgcca agcaagatac
     8581 tagaaactgt ttttctatgt tttttaaaca ctgctgttcg agccaaacca aaaacaatca
     8641 aactagccag acaaaaaaga ataatcaaaa atagagaaaa caaaaggatt gagataatga
     8701 tagtaagcaa aatccctaga tcagctcgcc atccggttaa aataaacatg actagaagcg
     8761 gaaaaacaaa aggaatgatg gtcaaagcaa ctaccaaaat ctttgctgaa aaaacctgtt
     8821 cttgtccaaa aggcagaggc aaaaatactt gcaggtcatt tccttcaaaa aagatattat
     8881 aaatgactga tatactttga gttaaaccta acataccaaa aagtgccacg taataagtaa
     8941 agaaacctgg caatttaaca aagttcatag caaacatagc aaatccgtaa atcagtaaaa
     9001 aaatcagccc tgaaaaaaga ttttgagaca aaagatagat agttaatcgc tttccctttt
     9061 ttcctttctc tcttgcttta ttcgtgagct gtggattaac atataaaaga ttaactcgaa
     9121 ttaattctaa taattgtcgc ttatacatac ggtccatctc ctgtcgcttg ccttccagcc
     9181 attttcaaat aaatcttttc tagcgtttca tcaggtgagc cattcaataa ttcactaact
     9241 gccccctgat aaatcaattc acctttcttc aaaatagcca gtttatcgca taactgttgt
     9301 gcggtatcca atacatgtgt agaaaaaatg actgtttttc cttttgcggc atgagccttc
     9361 atcatttctt tcaaatcaaa tgctgcttga ggatcaagtc cttgcaaagg ttcatccaat
     9421 acccaaatat ctggatctgg cagcaacgtg ccgatcaaaa tcgttttttg acgcattcca
     9481 tgtgaaaaac ttgcgattgt ttcattttga tgactataca tatcaaaaag cgctgtcaat
     9541 tctgctaaac gcttttcttt tttttgtgtg tctaattcat aggcagctga aatcaagtcc
     9601 caatattctc cagccgtcaa ttgtaaaaaa atatctgggg aatcaggcac ataaccgatt
     9661 ttttgtttga tactcaatcg attttccgtc aatttcattc catctactaa aatcgttccc
     9721 gatgatggtt caataatact aactaaactt ttaatcgtag ttgattttcc ggcaccatta
     9781 tgtcctaaaa atccaaaaat ttccccttgt cttatcgaga gagacaagtc ttttaatgct
     9841 tgttttttcc cgtaactttt acttacattg atgaattcaa tcatttctgt actccttttg
     9901 tttgttatta ctttttattg tacccttcgt tattggtgga gtaaatgtca aaaagagtgc
     9961 ataacaaaaa aggatagaaa cgactaggct gctacttggg ttgttctctt ctacaggaaa
    10021 acactcttct aacaggtaag atatgagtct ttgttttctt taaaatgatt cattttctat
    10081 cttttcttgc ataaaattct ttagtttatc aatacttctc tttcccatct ctagttgttc
    10141 tccatttgca aatgttacta tttcgtttac tctgctaata gatgtaatgt tttcagtatt
    10201 aatgatataa gcttttaaat ctttacataa atactcatat tcgagtgatt ttttatattt
    10261 tttaattgtg ccgtttacga gaatctcttc atctttcaat actaggccta ttttattttt
    10321 ataacctggc atgctcatta gataaaggat ttcatcgtaa tggatatagt gatcgctatt
    10381 accataacgg acagttgtcc accttttttt cgtagcatgt tgaacaggat agttttcttt
    10441 ttctttcaat aagtaacttt ggatgctttt tttatttttt tcataagtga attcttttat
    10501 caaataacca ctaggcgtaa tctttcgatt aataacagtt attgctttat tcggatgaga
    10561 cgttagataa acaattttac acttttcatt cttttctcta agcatttcgc ctagttcgat
    10621 accgctataa taggcgttca aatcgatgtc taggaaaaaa atatctgagg aatagatttc
    10681 attattttta atgaattcaa caaattgtat aacgttcaat ccctgaacga tctcaaattt
    10741 ccaaggactt aataaaattc tggattcttc caaaatttct actagcattt tttcaaaaac
    10801 caaactatca tccaaaataa atatacgcgt taccataaaa agtacctcca ctcgatattt
    10861 tactatcaaa atcatttcat acaacttttc aacatacatg ttaggaataa attgtaattc
    10921 tcttcacaat cttttccaac acctttgaaa ccgatatcaa atactttcag gatgctgctt
    10981 ctaaaataaa tatcaaacaa atgaatacat catgcaaaaa aagccagaca accgacgtcc
    11041 ttgcgtgtca gctgtctgag ctatgtttat ttattttgtt cttttaaaaa gaacgataca
    11101 catactgatt tttcggagca gagactttct cagcgtttgt aacaactaca gatgggattt
    11161 cacgcttgaa tggttgataa tagtctgatt ggatggttcc ttctatttta taccattcat
    11221 catttttaac cgttactcct tccggcatat gcgtcaacaa accaaagact cctgaatctg
    11281 ccacacaatg gataatgccg aatcggaaca agaagatatc tgtattgcta tcgttcggtg
    11341 tttggtaaac aaatccttca tagctgattt ttttgccaat gaattcgcta gggtaattat
    11401 aaattagctc cataacttct aaatagtttt catctgtgat cttgatagtt tgcttatccc
    11461 catacttttc tttcaactca ttcatttggt ctaagtagtc tgatttgttg aaataaatac
    11521 tggtatccgg ctttaagtat tgtgtctgca tctccggatc ccctactgat tctttactta
    11581 ctgggaaatt aaatcctttg gcttctacga tcgtagtgtc gagactaact gttggaaaca
    11641 acgtccccac gattactggc aaagctagca gtacataagc gattccacgt tgataaggtt
    11701 ttgacaaccc atggtcatga tcgtgctcgt gtgccttttc ttctgctttt ccgtttttga
    11761 cccacaaata cagctgaact aaagccaaaa gagcagataa aatcattgaa agaatagcta
    11821 agtagcgata atggacattg atatattgat cgagtttgcc agaaacttgc aagtacatca
    11881 tcaattcgaa atacccagat aaaatcaaaa accgcagcat attttccctc ctaaataaat
    11941 aatgtgaatc caataacgct aagggcaatc agtccgatca gcgataaaat aaagcgtcct
    12001 gtaaaatatc gtttcatcat cagtagattt ttgatatcga ccattgggcc aaagaccaaa
    12061 aaggcagcta caggtgctgt gccgaacaga cttaacaaag aagatcctat aaacgcatct
    12121 gcttctgagc aaagagacat cgtaaaggcc aataacagca tgatgataat ggctaaaatc
    12181 tttgttgagc cgagcgttag cataattcta gttggtaaat aagtctgcat cgcagctgcg
    12241 attaatccgc caatgatcaa ataacgacct gtatcaaaaa attcgtcgat tgaatgtgtc
    12301 aacacatgcc cagtttgatg gatcagctga ttttttggtt cattcttctc actttgttct
    12361 tcttcatgga catgatgatg gtgattttct gctactttag acgtgatttt ttctttcaat
    12421 acagcgtctt tttgaaaata ggcaagccag acaccaatca caagggcaac aaaaaagctg
    12481 cctatcaccc gccagcttgc catctgccaa gaattgccaa atgcgataaa agtggaaaaa
    12541 atcacgattg gattgatgat cggagcagtc agcataaaag caaacgctgt gtgaactggt
    12601 acgccttttt taacaaattg atgaacgatc ggtacgatgc cgcattcaca tgaaggaaag
    12661 aaaaatccta agatcgaacc agtaaaaata gagaagatac gattttcagg aagccatctc
    12721 ttcacacgtt ctggggtcaa aaatacttgt aaggcgcctg agataagaca gcctaataat
    12781 acgaaaggta atgcttcaat aacgatcgat aagaaaatcg tactcatctg caaaaccgaa
    12841 tttggaataa aagaaaacat gagtttcctc ctttttattt ctagttcatt cgctttgcaa
    12901 atatacgctt ttattcaaaa aaaacaagtt gctctgtaaa tttttacaca actcttattt
    12961 tttaccaaag cacttcaatt gactttttct gttattccca ttaaattaag gtctcataca
    13021 tatcacttct atcataaaag ggagaataaa aatgaaacaa accactaact tgactgaagt
    13081 tcaagacata ctgcaacagt catccgttgc cgtcatttat ctttcgatgc ctaattgcag
    13141 tgtttgtcac gctgttcgtc cacgactaga ggaacttctc acacattatg atattccggc
    13201 acttcatttg gatgcatttg aaacaccaga agtagctagt aaatttgaag tgctgactgc
    13261 gccagttgtc ctgatttttc atcaaggaaa agaagtcttt cggcaagcac ggttcatcga
    13321 ctttaaaaaa atccgttatc tactagatga actgacagca gaagatgatt ccctttctta
    13381 tgaagaaatc ttccgaacaa aataatcgtt gtccaaacag aaaaaagcga ttgaacaagt
    13441 gaaagttttg ccttcatttg ttcaatcgct ttttattttt attctgctgt attttcggaa
    13501 tgcgcatttg cgtcagaatg caagaaatag atcaaggttt gcaattcatt cgtcaaatca
    13561 acattttgga tcaatacatc tgacggagca ttcaatcgag cagcggtaaa gttcaagatt
    13621 cctttgattc ctgcttccgt taactgattg actacttctt gtgctctttc agcgggtatc
    13681 gttaaaattg ctacttcgat ttgttggatc ttgatttgtt ctgacatatc ttccattgga
    13741 taaacgggga taccgtctac gatgcgccca acgatttcct cattcacatc aaaagcacaa
    13801 ctgatacgta tgctattact ttgatggaat ttgtatttta gtaaagcact tcccaagttc
    13861 cctacaccga tcagcgcaac atttgtcaat tgatcgtcgt ttaatgtctt tgcaaaaaag
    13921 tgcataagat cttctacatc ataaccatac ccacgttttc ctaattcgcc aaaataagaa
    13981 aaatctcgac ggatcgttgc actatctact tgaacggctt cacttaattc agttgaagac
    14041 acttttttct ttccagcatt atttaaaatc cttaaatagc gataatacaa aggcagtctt
    14101 ttagctgtcg cctttggaat aatatgatct ttcacgaaat gacctccacg tattgtctaa
    14161 ttacatcttt gatttctcca tcatagcagt atttatagga aaaaatcaat tccattgctc
    14221 agatattgtg aaattttatt ctttattcat ttatttatca tttctgcaaa tctcgcagta
    14281 taatggaatt tatggtagac tactaattga aaaatagttt tttatttgag gagaattcac
    14341 aaagtagaaa agaggattcc atgatattat tacaagccaa tcagattgct cgccattttg
    14401 gtgcagaagt cttatttgaa aacatacatt tagaaatcag tacaggcgct cgtgtcgctt
    14461 tagttggcag aaatggcgct ggaaaatcga ctttattaaa gatcattgct ggaattgaag
    14521 cacctgatga aggaaagatc gctaaaaata aaacagcgac tctcggatac ctcgctcagg
    14581 acacaggact tgattccaat gaaacagttt ggaacgagat gctgaaagct tttgaacaag
    14641 ttagacaaat ggaagagcgg atgcgcgaga tagagatttt cattagcgaa ggtacgccag
    14701 acacacctgc ttatagttct tatttaaaag aatacgaccg gctgcaacac gatttttcag
    14761 atcgaaacgg ctatggttat gaaaatgaaa ttcgttccgt attgcatggg tttaaattcg
    14821 attcttcatt ttatgatcag aaaatcgata ctttatcagg cggacagcgc acacgtttag
    14881 ctttagctcg catgcttttg caacaccctg acattttgat tctagacgaa ccaacgaacc
    14941 atttagatat cgagacactt tcctggcttg agaattatct gcaaagctat actggtgcat
    15001 tattgattgt ttcccatgac cgttattttt tagataaagt agtcaatgaa gtctatgaat
    15061 taagcagaag aaaaatgcgc cattacaaag gtaactattc gaaatatcta gatttgaaag
    15121 cagaacagtt agcaagtgaa tggaaggctt atgaaaagca gcaaacagaa atccataaat
    15181 tagaagactt cgttgctcgg aaccttgttc gcgcctctac cacaaaacga gcgcaaagcc
    15241 gcagaaagac attagaaaaa atggaccggc tggatcgtcc tcaaggggat gaaaaacaag
    15301 caaactttct attcagtata gaaaagcctt ctggaaatgt agtcttgcaa atcgaagatg
    15361 ccgctatcgg atatgataat gtcaccttat ctgaaccaat caatctagat atccgtcgtc
    15421 aagaagcgat tgctctagta ggtccgaatg gtatcgggaa atcgacgttg ctgaaatcaa
    15481 tcattcacca gatccctttt atccgtggaa aagaaacgct agggacaaat gtcaccatcg
    15541 gctattacga tcaaggtcaa gctgatctgc atacaaacaa gacgatccta gcagaattgt
    15601 gggatgagca tcctaccact ccagaaaaag agatccgaag tgctttaggt gctttcttat
    15661 tttctggcga agatgtagaa aaaccaatcc ctctgctaag tggcggtgaa aaagcacggg
    15721 ttgctttagc caagttagcg atgaacaaag aaaatttttt gattttggat gaaccaacaa
    15781 accatttaga tattgacaac aaagaagtat tagaaaatgc attgatcgat tatgacggca
    15841 cactgctttt tgtttcccat gaccgatatt tcatcaatcg gatcgcgaca aaagtcgtcg
    15901 aactctctga aaatggcagc aaagtctatc taggagacta tgattattat ttagagaaaa
    15961 agcaggaaga agaagaaatc gccgaattgt tggcacaaga tgagccagaa tctgctcctg
    16021 ctcccacgaa caaatctgat tattaccaaa gcaaagaaca gcaaaagctt atccgtagcc
    16081 ttcaacgaaa aattactcag gttgaagaag aaatggcgcg gatcgatgaa tggatcgatc
    16141 agttggaaca agaaatggtc gcgccagaaa acctggaaga tcacgtaaaa ctcaatgagc
    16201 tgaaccaaga tttagaagct gctagacaag atcaagaaac aaaattgacg gaatgggaag
    16261 aactaagctt ggaattagaa gaatatgaaa attaacgaaa aaaccaaaac agaaaccatt
    16321 catgctgttt tggtttttct ttttttattt tatcaaacaa aaattccata tttacctttt
    16381 tcaccaataa gaagaccaat tactagtcat tctcactaaa gcttctaaat aatagtaatc
    16441 accccaaata caaggtgtat ttaccccatt atttttgggc ttattataaa cagactgtgt
    16501 taaaaagcca tcttcttctt ccgttgtagc atatctttct ttcaattgtc gcataatttt
    16561 ccaaacagca gactggtata agttcttttc tgttttgtct aatagacctg attctattaa
    16621 ttctaataat ccacaaacca tgatcaccat actagatgta tctttttctt ctctgctaga
    16681 atcatcaaag atcaaatccc aataacaaat ggtatcttct ggcaaccgat tcataaaata
    16741 attggctaat attttgctta aatcaaaata tttttgttct ttcaaataac gataacttaa
    16801 tgcaaaaccg taaatccccc atgcttggcc tctagcccaa caggaattat cactataacc
    16861 ttgatgcgta cttccatatc ttggttttcc atctttgaca tccatataaa atgtatgata
    16921 agttgaacca tcttctctta ctaaataatc tgctgcattt tgaatatgag cctgagcagc
    16981 ttttttatat ttttgatttc cagtttcttc actcgcccaa tacaataatg gaagattcat
    17041 gcaacaatcc atgatcattc taccttgttg tgaaggatca ctaggatctc cccaagcttg
    17101 tataactcct gccttctccc aaaaacgctg aagtaatgtt tctgcagcat caattgaagc
    17161 ttccttcatt tttcgatttc ccgttatttt ataatctgct acggttgata aagaatacaa
    17221 aaaaccaaga tcgtgatgat tagtgataat ttgacagtca atcctgtgga taaaatctat
    17281 atctgctttt tcagctgttt gttgaaataa aggatcaccc gtcttctcaa ataacaacca
    17341 attaattcct gtccaaaatc cttctgtcca accaatattg tctcttaatg gataacgtcc
    17401 ttttatagga cactcgcttg ggaattgata ctgactgtta ctgatacgtt tgattaacct
    17461 ctccgtgtgt ctgcgacaac gctcgatctc attattccat tctttttcca ttaattcttt
    17521 ataacattca tttatccatt tttctggatg tattatctct tcaaacatca ctttatcaag
    17581 catttcccca ccagccttta gcataatttt agtctttttt tattgttttt attttggata
    17641 gttagcccgg ttgtcccatc aaaatcaatt ttgggacgat tgttccatac ttctaaacta
    17701 taatcccgat cttgatgagc aaccactcct gttccgagcc aatgggttcc ttttccaaat
    17761 gtaccctcta atccaggaat aaaagtgttt tcccatgtca tcaagtttgt attgggcaaa
    17821 cccataatag ctgtggattt tcgtttaata gtatcgtcac ttaaacagat aatacctgaa
    17881 aagccagctt cactcaccct atagttttcc ttgtcttctt ctatgattat tccttctttc
    17941 tcaggagaac attcaatagc aaaccctgtc tcatatagaa catattctgt atctaattct
    18001 actttgtgta cgcgaatctg ccagccttca aaaggaataa gccaagttct gatttttgcc
    18061 tgtaaagtag gttcccatgt agaggcaagt acctctttct tacatacaat gtctcgtaca
    18121 ttatttcgaa cgatgaactg tccataggtt ttgatgctct cattcttaaa actggtcgtg
    18181 atatatgtgt ccgctcgctg aatccctagc attgaatcca ttgccatttc tgctttcaat
    18241 tgattcgctc gcggtatcga aaaaccaaat tgactagaat aagcaaattt taaatatttt
    18301 tcattggtca atgtatggaa atttggccct aattgtcccg cattcaaaaa aaatgtatgt
    18361 ccattatcat gttgtatgag caaattggct tctgaaagaa gtttagttcc ttcacgaata
    18421 ggcataggca tttctttagc cgtccaatac tcatggtctt caggtagcgc caacaataaa
    18481 aaaatcttat tcagccataa tggagataac gtactattat agggttcagt caatgtcaat
    18541 tgagggtagg tataaccaat attcaggata cctttttcat caaatatttc tttttgcatc
    18601 caccatctta tattccgatt gactaacccc ttgatttcac ctaatttaaa aggcaaaaga
    18661 tcggcccaca acatagctga ccaaaagctt gaaactgcaa aacgataaat catgcttcga
    18721 ccaaaaggca catttgcacc atcttccgca aaaaaatgaa tatagtctct agaaaaatca
    18781 atagctcggt tcttatatat ctggctaatg ggcttttctg gttctagtac gctataaatc
    18841 aaaccataat aatgaaatgc aaaaggatta taataatctt ggcgacctct tgaactgtct
    18901 cgataccagc cattttgaag ataacaattt tctatattat caaatgcttc tttcagtgct
    18961 gattctttaa ctggataacc aaatttttgg ataattgttc cgacaattat cttaaaaaat
    19021 tgccaattac catctgcaca ttcatattca aaaatttgcg agaaccaatg aataatctgg
    19081 cttttttctc ctgtatctaa ttttttccat aataattgtt catgatgata taacgctaat
    19141 aacattgcgg gcatttctac catacgctgg tctctttcat gtatttctcc ccaataatcg
    19201 ggatgggaag gatctgttcc agcttttatt cctgatagaa ttaatttcaa ccatcgttca
    19261 tctaattggt caattgcggg ccccgctccc cataaaaccc gagagaatcc ttccattcct
    19321 gcaattttat ttccatatcc taccccttga tgtatataat caatttgtgc gccacttgct
    19381 cgataaaacg gctggagtgg ttgatttatc tttttaaata agtcaaagta atcctgtctg
    19441 gtccttaata gtggtactgg atgtttcatt gtattttctc ctctttttac taccaaatag
    19501 ctaattccga ctgttctaat cgaagtaagc cttcaataaa aaaataatca gaatagatca
    19561 gtggcacatt ataagtcagt tctctatggt atgcttctgc tccgcctcca atgatcccat
    19621 cttcatccat attccaattt gcatagtgct tttctgtctt ttggagtata ttgatagcag
    19681 cttcatgata catttttgat cgtacagagg aaacatatcc tgctaattcc agaagaccgc
    19741 atgcagcgca tactccagca ctggtatcaa tattcgaatc ttttacagca aaatcaatga
    19801 gaggaatata accagttttt tcgattttct gaataaaatt tactgctatc ttttctgcta
    19861 tagctagata gtacggattt tttgtgtaac gataacttaa cgcgaatcca tataaagccc
    19921 atgcatttcc tctgctccaa caagaattct cattgtatcc ttgcccgcca agtaaccgaa
    19981 tatatttccc tgattctgca tcaaattctg cgatatgccc ggttgatcca tctttcctca
    20041 ctagatactt agctgttgta tctgcatgct gccgagcaat tttagagaac ctcggatcac
    20101 cagtttccct cgaagcccaa tacagtaatg gtaaattcat catactatca ataatcatcc
    20161 aaccaggttt atggtcattc catgctacta aatagttccc tgaaatatta tagcgtccag
    20221 caagtaccgt agctgcatgt aaccctagtt gtcgactatg tttgttttga gactgttgat
    20281 aatgtgcaat agccgatggt aagaataaaa aacctacatc atgatgaacg tctgaaaaat
    20341 ggtacagggc agattctaat aattgttctt gttgtattgc tgcctctttg aacttttctc
    20401 gttttgtctt gtgaaagagc tgccataaaa taccactcca aaaaccattg gtccaccagt
    20461 cgattcctct gtcctttatt acgtctgtat attttccgtt gtaagaaata tatggaattt
    20521 tattccccat tctgtctaat tccgcatcta tcttttcaga agctacattc aaaatttttc
    20581 ttgaccattc cttcgtctgt tcattcatgg atatcccgcc attcttttaa tttttatgaa
    20641 tgtggagttg ctcctagaac gaactagatg gttaacattc gttatttttt ttacttattt
    20701 gcagctccaa tgtcattctc cttaaattta gactgcgccc acacataaat caagaccatt
    20761 gggatcactg aagttgctaa aattagcata attgcgccat aattaattgg ctcacctgta
    20821 ctagaaaccg tcatattttt gataattaag ggaaccgtat atttatcttt atctgaaagc
    20881 atgactaatg gaaatagata ttgattccaa cttcccataa agataagtaa accactagta
    20941 ataatcgtag gtgataaaat cggtacagcg attttcgtaa aaatcaccca ctcattggca
    21001 ccatcgattc tggcagcctc catcagtgct gtaggaaatt gactgctata ttgtcgcatc
    21061 ataaacactg aagttgtact ggcaatcgaa gggatgatca cacctgcata agtattcgat
    21121 aatcctaaac gactcataat gtaaaataaa ggaatcaatg tggtaaaaaa aggcaccatc
    21181 ctagatagca taactgtatt gaacaacagt ttattcccca taaaacgata ctttgctaat
    21241 gcataacccg ccattgtaga actggctgta gctgaaactg tactgattac agcaataaca
    21301 atcgaattcc aaataatcaa gggataatta tatctcgcca tgatggcttg ccagtttttt
    21361 aatagcatat ccccaaactt gaaattgatt ccgccactta aaatttcttg attactttgc
    21421 gttgaagcta caatcatata taaatatggc atcaaaaata ttaaggcacc aaacacaata
    21481 atgacgtacg taaaaattct gctttgtatc ttttgtttag tcatttttat cacctccgaa
    21541 tttaaattga atagccgata atataacaga tacaaacacc atcgtccagg caatagcaga
    21601 ggcttttcca aaatcaacca tttcaaaact tgttttatat aaattgactc ctacgaccaa
    21661 agctgaattt tctggtccat tggaagtccc aaagaatata tttgggatat tgaaattacc
    21721 caagccattg atagtggcta aaactgtggc cattaaaatg attggcttta gcatggggat
    21781 cgttaataaa aagaattgcc gaaatttcga tgctccatct atttcagcac tttcgtatat
    21841 ctctttagaa atcccttgaa gaccagctaa aaataaaata gtgttatatc ctacactaag
    21901 ccaaatcatc gtgatgatca ttgatattcg tatccaaaaa ggatttgtta accactgtac
    21961 accgttaatt cctagcgcat tcaaaaatcc attgaataac ccagtcggat tgaaaaaagt
    22021 catgaatacg gaagctacag ccaccgttga tgtaatggtt ggaatataat agacagttct
    22081 aaaaaatcct tttcctttta actgtgctga attcaataaa acagcaaagt aaatagctaa
    22141 tagcatcgtt attggaattg atcctgccat gataatcaaa acattcgtgt aagatttaat
    22201 aaacaatgga tctcggaaga tagctagata attgtcaaat ccaacaaatt caaatgagta
    22261 gccaattttc ataaaactaa aagcaaacga gataatgatg ggaatcaaaa tcgtcacaat
    22321 aaagaaaata acaaaaggtg tgaccaatcc ataagcactt tttttatgag acgtatctgt
    22381 tgcttttttc ttgctgttct ttattgtctt gcttggtaac gaagattgca tcatcgttaa
    22441 gtcctcctaa tattttttaa tcaccagtta attttacgcc gtacttatga gcaaaatttt
    22501 ctgcttcttt tagatatgaa ttttcaaaat cacctgtttg ccaatagtta tatgcagcgg
    22561 tagtcaaata agacgtagca tctgaataag tggctggtaa aacagtattt gatgtatttt
    22621 cattgatttt attcaatagt tcatagtatt tttgtcctcc ttagtagtca aactttttct
    22681 cagctgcttc tgtttgatag gccaatgagt tagatgtagc cacgcccagc tccagtgcgt
    22741 tggctaagca ttcttcatct gtcagcataa atgtaagaaa ttgttgtgcc acttgtggat
    22801 ttttggcatc tttagggaca taaaaacttg atcctcctga tactgcacct tttcctggat
    22861 tttcttttga aaaaggaatc agtggagcca tgcgccattt gtcagatgca tcaggaaaat
    22921 tcaaagacat attcgtagca taccagccgc cagcaacaaa cattgcagat tcttgaaatg
    22981 ctttctcagc atcttgtgca ccgtagaaat gtacaatatc tgagtcgatc atttctttga
    23041 ttaactttgc agcatgtttc gcttctttag tattcagacg gatatttcct tcttcatcaa
    23101 gcaaagattg attctgctgg gctagcatgt tgataaacaa ttcttggtcc ccagattctg
    23161 gcaaggcaat tccaaaacta ccagtttttt cttttaatgc ttttgccacc tggatatatt
    23221 cttcccaaga actgatttct tcaaaatcta cacctatttc agcaaatttt tttggttgat
    23281 aataacaaag tgctggggta aaatcactag cccaaggaat aatcgtttca ttactcgttt
    23341 gttgacgcaa aatattcaat ttttgatcat aaaactgatc gccatatttg tcataaaatc
    23401 catattcatc agcactataa aaagcatctt gaaatttttc cagcacacct gctgctgaag
    23461 tgtctgccat ggagataata tccggcatat cttcgtgtgt taccaatttt ggggtgatca
    23521 tttgtactgt tgctgttccc gaagctacat tcacaatttt taagtcaata tttgctccag
    23581 ttttgcggtt atagattttt gcagcatttt caacatagtt tgtcagtttg ccgtttgcga
    23641 catatacttc taatgtagtg gcttttttta tcattggatc tgcgatgttt gctgttactt
    23701 cttcgttagt ttttccacat gcacttaagc tgaataatag ggtaatgttt agcaaagcta
    23761 tcaccaattt gtatcttttt ttcatgtcta tcccttcttt cttttcaagt tcttatataa
    23821 aacgctttca cttatgtata ttaacggaat tagtaacaca aatatttata taaaaaaata
    23881 ttttacttgt cgttttggct tttcattttt gttataatag tcattaatta taaatagaaa
    23941 gctttttata taaaaagaga aaggaaagtg gttcttttgt taacaaacaa cca
//
LOCUS       DACDIE010000040        23482 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.040, whole genome shotgun sequence.
ACCESSION   DACDIE010000040 DACDIE010000000
VERSION     DACDIE010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.040"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(349..1056)
                     /locus_tag="IV250_001745"
     CDS             complement(349..1056)
                     /locus_tag="IV250_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769028.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENG"
     gene            complement(1121..1957)
                     /locus_tag="IV250_001746"
     CDS             complement(1121..1957)
                     /locus_tag="IV250_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8769029.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(1975..3384)
                     /locus_tag="IV250_001747"
     CDS             complement(1975..3384)
                     /locus_tag="IV250_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8769030.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3398..5221)
                     /locus_tag="IV250_001748"
     CDS             complement(3398..5221)
                     /locus_tag="IV250_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8769031.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(6064..6390)
                     /locus_tag="IV250_001749"
     CDS             complement(6064..6390)
                     /locus_tag="IV250_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769032.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(6436..6798)
                     /locus_tag="IV250_001750"
     CDS             complement(6436..6798)
                     /locus_tag="IV250_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAP8769033.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(6835..7050)
                     /locus_tag="IV250_001751"
     CDS             complement(6835..7050)
                     /locus_tag="IV250_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8769034.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            7177..7512
                     /locus_tag="IV250_001752"
     CDS             7177..7512
                     /locus_tag="IV250_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8769035.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(7588..9585)
                     /locus_tag="IV250_001753"
     CDS             complement(7588..9585)
                     /locus_tag="IV250_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769036.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(9683..9958)
                     /locus_tag="IV250_001754"
     CDS             complement(9683..9958)
                     /locus_tag="IV250_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8769037.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            10190..10924
                     /locus_tag="IV250_001755"
     CDS             10190..10924
                     /locus_tag="IV250_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8769038.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            10963..12105
                     /locus_tag="IV250_001756"
     CDS             10963..12105
                     /locus_tag="IV250_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8769039.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(12200..13591)
                     /gene="sufB"
                     /locus_tag="IV250_001757"
     CDS             complement(12200..13591)
                     /gene="sufB"
                     /locus_tag="IV250_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAP8769040.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKAVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(13596..14066)
                     /locus_tag="IV250_001758"
     CDS             complement(13596..14066)
                     /locus_tag="IV250_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAP8769041.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(14053..15288)
                     /locus_tag="IV250_001759"
     CDS             complement(14053..15288)
                     /locus_tag="IV250_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP8769042.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(15285..16571)
                     /gene="sufD"
                     /locus_tag="IV250_001760"
     CDS             complement(15285..16571)
                     /gene="sufD"
                     /locus_tag="IV250_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAP8769043.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(16584..17357)
                     /gene="sufC"
                     /locus_tag="IV250_001761"
     CDS             complement(16584..17357)
                     /gene="sufC"
                     /locus_tag="IV250_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAP8769044.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(17554..18402)
                     /locus_tag="IV250_001762"
     CDS             complement(17554..18402)
                     /locus_tag="IV250_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8769045.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(18426..19112)
                     /locus_tag="IV250_001763"
     CDS             complement(18426..19112)
                     /locus_tag="IV250_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8769046.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(19105..20139)
                     /locus_tag="IV250_001764"
     CDS             complement(19105..20139)
                     /locus_tag="IV250_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8769047.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            complement(20534..20872)
                     /locus_tag="IV250_001765"
     CDS             complement(20534..20872)
                     /locus_tag="IV250_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAP8769048.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(20874..21230)
                     /locus_tag="IV250_001766"
     CDS             complement(20874..21230)
                     /locus_tag="IV250_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAP8769049.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(21236..22423)
                     /locus_tag="IV250_001767"
     CDS             complement(21236..22423)
                     /locus_tag="IV250_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP8769050.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(22614..23459)
                     /locus_tag="IV250_001768"
     CDS             complement(22614..23459)
                     /locus_tag="IV250_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8769051.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
BASE COUNT         6874 a         4968 c         4007 g         7633 t
ORIGIN      
        1 tttcataaaa aaacagtact gtctagtagt ataaaatata caaatttctt aaaaattttt
       61 tcactaaaat cactacctga aacagtctaa aacacttata tatcaacgtt taatgaaact
      121 atctccgttt catttcagat acaaataaat atagtcgggc tccaaatccg aaaaaaaata
      181 cccccagtag cttatcctaa gctactggag atgtataagt acggtcatat aacaacgggg
      241 tagggtaaat aaatgaaaaa gatggaagaa tcattattca ataacttttt taaatacttc
      301 gttgtaatca gttacctgtc cgttcttaaa taccacagaa taatcttttt acccattttc
      361 aagtaaccga agtggaatgt ctttaccaac ttgctctgtc actgcattac tgatacctgt
      421 tagattagct aataaatctg ataaagattg agcatctgta gaaatctctg tatcagtatt
      481 tatcgtgatt cctttatctt catttacaag cttccaagta ggtggtatag ctgtgtatgt
      541 ttcgatgaat gaatcaattc tttcatctat cgtcttccca gtatctactt ctgtctcatt
      601 actcttagat gaatcgattc tttcatccat cgtctgctcc gtaactgctt ctgtctcatt
      661 attctcaccg gaaactctct tcacacccaa acttatatat tgtccatctt tagtgtgcat
      721 agttaaaatt cccacgcttt tactaataaa ttcactataa ccagttcttt caatagagac
      781 ttcaatgtat ttcacactat catactcaaa agaaaaatag acatgtaaaa gcattttgtt
      841 gtttacatct acataataat cctttgactc tacttgagac cttcctatac ggaatacagc
      901 tttactgtct gatatctcta taccagcatc cttattatta atctgccaat ctccaagtag
      961 ctcttctggc aagctagtag tttcttgtga acactttacc acttcactgg tagaggtcga
     1021 aacattcacc tccgcactgt tagaagaatt ttccaaggaa tcaggagcct tagcacaacc
     1081 aacaagtctt tatatattgt gcagccgata ttttcttcat tcagtacacc cttgaaatat
     1141 gcagtgttag ataaagcagt tcgtcctgat ttgctgaggc acccatctct tcttgaacgt
     1201 actccttgat tttcaaggcc gcccgatact ctttcggaaa acgctgcttc actgaagcca
     1261 gcaaaaaatc atccttctcc ggcaactctt ccttcttaat cattcgggca ataaaaaact
     1321 gtaaatgcgt cacaaatctt aagtaaagca tcgaatcttt atcaaccttt ttttcgggaa
     1381 acagatatcc tagcatagcc aagatatcct ttagtgctct taccgagagc atggtttctt
     1441 ccatatttgc ttgttcactt tgggcattta caatgtgaaa cgcaatgttt ccagcctcct
     1501 cttctggcaa ctgaacattc atttttttgt tgatcaattg gatagcatag cagccgatct
     1561 caaattcttt tgggtaatac ttttgaattt caatcagcag gcgattttga aaaacaatgc
     1621 ctttttgact gcgttcaatg gcatgcttca agtgatcaac caaggtaaaa aatagacttt
     1681 tattcagctt aaagtcaaac ttggcgttgg cataatcgat aattcgcttg acgcaaataa
     1741 ttagatcatc cggagcagcc tcaaacagtt taatgtaatc cttttttaga gcatcagaaa
     1801 gtgaatctaa tttaaatact ttttggattt tgtcagccgg aaccaaatcg cctttcttta
     1861 gttgaaatcc aatccctttc cccatcaaaa ttacttcttt gccttgctta tctaagcaaa
     1921 gaattaaact gttgtttaac gttttaatta ctctcattag attcaccgtt ctctctaagc
     1981 taagccattt cttgcgatta ctttttgata ccaaaagaag ctttcttttt ttattcgttt
     2041 taattcttta agattctcat cggttcggtc aacatatata aaaccataac gcttattgaa
     2101 gccttgatgg gtactaacaa catcaatcgc tgaccaaggt aaataaccaa aaacatctac
     2161 accatctgtt aaagctgctt gaatagccag aatatgtttg gctagataat caattcgatc
     2221 ctggtcatta attcgtctat cttcctcaac tatgtcgggt gccccatagc cattttcagt
     2281 aattaaaata ggtaaatcat agcgatcgta caatttcctc aatgttaatc gtagtccagt
     2341 agggtcaact acccagccat atggcgtttt atctacataa ggattttcag ctgcccggta
     2401 gacaccttct tctcccaaca tgatctgttg atcccccgct ctagcggaaa catctgatcc
     2461 gtcatttttg ctggcagcga tggtggcagt tgaataataa ttaatcgcaa taaaatcggg
     2521 tttaccggag gataaaattg cctgatcctc tgcctcaatt ttaggatata atccacgatc
     2581 attcaaatag ttttcaaaca aataattgta ttttccccga actgctacat ctaagaaaga
     2641 ccagcctctc aatgtttccc aattatgggc tgcaattgca tcttctggac tggaggtagc
     2701 ttgatacata gccgttaaat tgattgctgg accgattttt cctttaagat tcaactgatg
     2761 atacaagtga attactctag cttgagctaa taaaacatgg tgattaattt gaaagagttc
     2821 tttttttgaa ggcagctctc cacttggtgg caaaccaatc gctccaggat gaaggatcat
     2881 cgtgttttgc tcattaattg tcagccaata gttcaccttg tcaccatact cagtaagcaa
     2941 tacctgtaca tagtccacaa aataatcgat agttgctcga tttaaccaac cgccctgctc
     3001 ctctagaaaa taaggtaaat cgaaatggta catggtgaca atcggttcga ttccctgatt
     3061 caggcattca tcaattactt gatgataaaa agaaattccc ttttgattgg ttttattatt
     3121 ttcaatatcc ataattcgcg tccaagcgat ggaaaagcga aaagctttta agcctaattc
     3181 tttaaacagc tgtatatctt ctttatagtg atggtaaaaa tccgaggcaa cttcaaaatt
     3241 agctacccct tctgtatgct gatacttgtc aataatagac ggttgtttcc catcctcctg
     3301 ataggcaccc tctgcctggt aggcactaat ggaggcaccc cataaaaact tctctggaaa
     3361 tgctgaaagc tgtttgtatt gcacaaaaat cctctcctta actaatttgc atcaatggtt
     3421 cgtgttcctt taagcttccg cttgcttcac taattcctgc gtaattttca ctgttggtca
     3481 ctaccacaat cactgtatca tcgtagccag cgtttctaat ggcagcgaaa tcggcgttga
     3541 ttaataattg tcctgctgac acctgatctc ctgcttttac aaatacttca aagggttccc
     3601 cttttaaatt cacggtgtcg atccccacat ggatcaataa ttcaatacct tctttagaac
     3661 gtagaccaat cgcatgttta gattctaaaa tagtaatgac ttctccctca acaggtgaat
     3721 aaatgctgtt ttcttctgga tgaatggcta cgccctttcc caatagtccc ttagaaaaga
     3781 cagcatcgtt cacttcagaa ataggacaca cggttccttt taccggattt tcaacaatga
     3841 ctgcttcggc tattgccgtt tgctctgatt catcagaagt atcgtcaaat ccaattagat
     3901 aagcaatgat aaaggttgca acaaaggcga ttacacacgt aataattgcg tgaacaatat
     3961 tcattggatt ttctccaata aaggctggta aggctgctaa gccaggggaa acaaaggcat
     4021 atctgactaa accgctcaag ccagcataca aaccagcaat tgcaccacca atcatggaag
     4081 caactaatgg ttttttcagc ttcaaggtta ctccatagag tgaaggctca gtaattccca
     4141 ttaaagcagt gatcccagtg gacacagcta gctgcttcaa ctctttgttt ttagttttcg
     4201 cggctacagc aaaggtagca cctgattgag caatattaga agccagcatt cctggcccat
     4261 tgatcatttc agaacccgta ctgctcaatt gcatagtagc gattggcgtc attgcccaag
     4321 ctgtacccgt aaccaccaaa aatggctgca aaccacccat caaaaatgga attaaccaag
     4381 ctgctttgcc gttaatccat tcagccccca ttgctataag atcgttcagc aacgttccaa
     4441 acggtccaat gacaaccaac gctaaaggcc ccataattaa aaataccagc atcggcttag
     4501 taaagaattt aataaacgaa ggcgaaattc gatccgcaaa gggctctaca taggacatga
     4561 tccaaacagt aaagataatc ggtaaaactg atcccgcata atctgcagct aataccggaa
     4621 ttcctagaaa agaaacggca tcgccagtag aaaaaagctg tgaaatattt ggatgaagca
     4681 aaatgccagc aacagtaatc gccaaaattg ggttgacatc aaactttatg gctgccccat
     4741 aggctaatag tactggcata aaataaaagg ctgcatcagc aatagtattc aaaatgatgt
     4801 aagtttgact actgtctgat agtacaccag tcaaagtcaa gacggccaaa atggctttta
     4861 ccatcccggc acctgttata gctggaatca tcggtgtaaa ggtggtagaa atgacactaa
     4921 tcaatcttga aataatactt tcttgattgt ctgaagaggg gcccttttta attgaagttc
     4981 ccacttcctt ttgaatttcc ttgaaggctg tttggacttc attgccaatc accacttggt
     5041 attgtccacc gctatccacc acactaatta caccaagtgt tttttctaag gctttcgtat
     5101 cggctttcct tttatctttg agttcaaaac gcagtctggt ggcacaatgg gttaagctga
     5161 ccacattttc ttttccccca acctgttgca atacatttct tcctacttct ctgtaatcca
     5221 ttattctctc tccttaaagc agtaaaaaag accaagtcaa accacgcccc aatcaacatt
     5281 gaagtgttag tttgtcttgg tcttgcctgc ataatcagta acatgccaat aacgttattt
     5341 tgctgtaatc caataatagc acattatgat agcgcaatca acagggaatt taatttttta
     5401 tcttttcgtt tgaccgtata gggtaatata acagttagat cacatcaact agggtatatc
     5461 tgcgtatact tactgctaaa ataagtatat ctaatattaa ctataccagt aaaaccattc
     5521 ctacataacc agcgggaaat tcccaaaatc gtgaaagcat caaaaatgca tataacggat
     5581 cacacaactt caacaattct gctcttggga aaaattgatg aatttggtga gtaaaggatg
     5641 gaataaacag cattccaaca atataggcaa caatagtgat acttgttttt ttggtaaaaa
     5701 tcagtaagca atagccaatt tcaatcattc cgagatataa aggtatcgat agaatgattt
     5761 gggctagtat tcttaaaaat taactagcaa atgtttaaac tgatttattt ctaatctaca
     5821 tgtagtaagt atttcaattc caccaattat caataaaaat accaaacata tccataataa
     5881 ttttttcttt ttcatttctt ttatattgtt ataacagtga ataacataaa aattcccgac
     5941 aatcattgta atattttttt gtcacctgta ttagtattta atttatagga aactataaaa
     6001 actccctagt gaatttacac ctttgggagt tcctgtttgg acggtatgca taatacattt
     6061 aaatcaattt aacattaaca cctcagcata tggtatctcc acatatatac ggtctttctt
     6121 tggatagtac tcaatattta tgttgatagc acttccattg tctaatatag ctttagcccg
     6181 gtatgtgtta tctccatttt catgagtaat cgtcacatca tcacatgatc gatttaagtt
     6241 gtactgttct tgtaaaatct gtgttaccaa tggttttgat gacttctcta atccagatgt
     6301 acctgtcatt tttgaaaaaa taatacctat aatagccaag acaacaatac ctataatgac
     6361 aaacttcttg ttcatattta ctttattcaa ctttttatct cctatctcca aaataatttg
     6421 ttcttatccc tcttatcagt acattgacat ccctaaaagt acaccaaaga ttgctccagc
     6481 tacccctgct atcatcaaaa tcgtacccat tttctcatct ttttctcgat ataagtagcc
     6541 cataataaca gcaacagcac caaatacaac aggaataaat gctaacgata ctaccataga
     6601 aatccaacca ataatcaaat acagcttccc tgtctcttgt ccatgtacga tttgttcctt
     6661 catttcatta attgtctgct cagtactttt ctttactgtg tctgttccgg ttgtctcatc
     6721 tgtttcttgt agtgctccac aaaactggca atacttactt tcatctgtta tttctttgcc
     6781 acattttcta caatacatgt cttctcctcc taaaatcatt caattgctta ttagttaact
     6841 gtttacttta ttaaatacat cagaaaacag ctcattcatt ggtacactta atatctcaga
     6901 aagcatcttc atttgatagg gtttaggaat cgttctccct gtttgccacg aacttacaca
     6961 ttgctgtgta acattcattt tttttgctaa ctccgtttga gaaagtcctt tttcttttct
     7021 gtatcggctg agattactta cttttttcat gattctcccc tcctgtttta ttatatacaa
     7081 caatatgatg tatttcaact cataaaatat aaaaaacttc aaataccatt acaaatacaa
     7141 caacttgttg tactatactg agatgggggt gtattaatga aacagaaaca atatgcaaag
     7201 gtcattagag aaaagcgaaa agctctcggg tggacacaaa aagaattagc tgaaaaaatc
     7261 ttctctacac aacaggcagt agcaagatgg gaaaattctg taacagagcc taacctcgat
     7321 agcttaacag ctctttctag agccctagga acccctgtaa gccattttct agacaatgtt
     7381 gtcgtagatt atgaggaaga gtttttggct ctctatcgtt ctcttagcac tgaagatgca
     7441 gttcgaacaa ttgactatat gaagctatta aaaaggcaag aaaatgaacg caatcagcta
     7501 ttgaaagatt gaaaaataaa taacccaccg aactttaacg gtgggtgtat tgagatgaaa
     7561 taagattaag agctatggat aacctgtcta gtttaataca attgggtctt ttctcttcct
     7621 tactctccgt ccatcatctc ttgttacact tgtaaattga tacagaggaa ctatcccagc
     7681 tcctgataat atcaattctt ttattgtttc atctatccct gcccctttcg ctaaatatat
     7741 taaaaaacta ctacttgctt tcacattgta tagtttctta atatccttag aagctcgttc
     7801 aagagtgaat ccttgtttaa caaagtagcc tctgtttttt attaaagcat ttttcacgct
     7861 gttatcccca acattacgta ggcgttcaca aacagtgtta ataagttctt tctctgtcat
     7921 tagatatgtc ccttgccacg ataagccctg taaaacttca tgtgcttcat ctaaaatcaa
     7981 ttgtttttcc ttgctgagtt ctgatcggga attttgagga aatactcttt tagcctcttt
     8041 ttcgttaaaa catatcttaa cccattcata agaaaaatat tgatatgtaa aatgaatttt
     8101 ttccagaagt ggaaggatat cttcattcaa tttttgaatc acagaagttg cattaaaaac
     8161 gtctaatcga tagacattgc ttctcttatg attaaagttt cctaacgctt tctcttcctt
     8221 taacctattt aggtattcga gcatcttcaa aggaacatct tcatcatcta atttttttat
     8281 caatcctata aaagtcataa gtgatagtaa cttattacat ttatctatgt cataagtttt
     8341 accatagtaa gaactcattt ttttagctaa ttgttccccc gacaaaatac aagtcacact
     8401 ttccttacta tcatcaaaaa aattaacatc agaaagcaag acatcataga cacgtcgcat
     8461 ttcctctaga taattcctta ttgtcctgaa aagtttgtct ttcattgatt caagagtgtc
     8521 aaaaagtact tttgattcct ctttcaattt tcgaaaatgt gtaggataca agtctaggtt
     8581 ctctaatagc caattaaatg tttctacccg actgtctttc tttaaaatta gcatcacaac
     8641 tccaataaaa ttttcactga tatcacaatt actggaatga caataaaaaa tggaatgccc
     8701 agttttactg gtaaaaatac tagcagaggg attatgatcg ttgtgaaaca gacatcttaa
     8761 gtttttactt gacttaagta atttattcat atcaatggtt aacaatcttt cataaatctc
     8821 attaaaagac atgtcggagg aatcaaacag gcagtcttta aaccactgtt tcatttcttc
     8881 tttgtcgtca ttttttatca acttaacaaa atcggtttca ttgactagtt catactctct
     8941 ccctgaatca ctttgacaat ttttagaaca tgtcatggta aaatcaatcg gaagctcagt
     9001 aatatctaac atgttgtctt cattaattat tatcactgca tctgacttat atcctccaaa
     9061 aaacattcta ttcgaagcat ttacattgtc atcacacccg gggatgttct ttttcaagta
     9121 ttcgtaaaca gcaaccattt taccatagtc agtaattgct tcttttaata gaaagacaat
     9181 cttgtatttt tctagatttt cagagtaccg aatacttttt tgtatcgcaa acgcagaatt
     9241 atgaactaaa ttgatagata aaaccttttc tatgcttaag tatctactat catctttact
     9301 caaatctact accttcccct tctcatcttt aacagtgtta tcgacatcca gaataattac
     9361 tctggtttcc acaaaactct ccttgtcatt gcctttattc tttgtcttgc ttggaataaa
     9421 tgattgtgat gaatttagta tctctacaaa ctcatctctt gtaacttctc tatagttatc
     9481 taaacaccta tctcttacat cagttttttc tttttttgta actggcttct ctaataattt
     9541 ttcttgatct acataaattg gaatattaat cattgatgtt tccatttgtg tttcctcctt
     9601 ataaaattct gttaaagact aaaacataca gataataaaa acaaactctt tattactata
     9661 tgttttagtc ttatttgttt actcaacctt tataccgttc catgaaacta tcaatatcag
     9721 attttttaaa tctaatgacc gtatcaattt ttataacagg taacccgtat tgagaaacat
     9781 attttctaat cgttcctact gatacaccta gatattcact tgcctgctga tagtccatat
     9841 actctagagt gactccagaa ataatgtctt ctagcatagg ctttagcact gtaattatct
     9901 ctttcacttg ttgttcaact tgtatagaaa caatgctatc aatgatttgt agagacatat
     9961 ttccccctcc tttcgtgatt gcttgaacaa attttctaag aattatcttc gtattttttg
    10021 ctcaaaataa aagtattttg ttattaaccg attaatcaaa caaaaaatga ttaccagttc
    10081 atcgacatac acataatacc tcgataaaaa aggggtgtca acagctttgt aaaatttatt
    10141 tacaacaccc cttttttgag gtatactaat tttaggaggt gtggtcaata tgataaaacg
    10201 tccaccaata aattatttag aacgaaaaaa gatactcgga acaaaaataa aagctataag
    10261 aaagagtaaa aaattaacac aaccagcatt tggtcttatg attaacaacg gtcaattaat
    10321 tgacaaaaaa actatctacg aatgggagaa gggaacctat ttaccaatac cagagcgatt
    10381 atcccgaatt gctgatttgg gaaatatgag tatagaggag ttagtttgtg gcaatgtaga
    10441 agaatatatt ttgggaatta ttctttatag agatagcatt gtactagatg gtataacatt
    10501 tccagataaa aacctgttcc agcatcttcg tcaacagttc ccaccagtac actctaattt
    10561 agatacatgg ctggatagat attccaaatt agaaccagag atgcaagaat ttattgctaa
    10621 caagacttgt aataaagtta aaaatgaaaa aattagtctt ttcaatatct taaaaataga
    10681 agaattattc atcaatgcta ttgtagaaga atttgacaat aacattcttt tcctcactag
    10741 cagtatagaa gaactactag aaagaatggt agatgaatgg ctacctattc aattgaaaga
    10801 catgtcttat cccgaagaag ctgtgagaga aattactgat aatataaaca agctagaaca
    10861 aacgatttca tcaattggga aaaaatacac taagaaaaaa atgaagggag gtgataccat
    10921 ctagtggtaa gtactccttg taaactaaga ggagatgatt taatggcaaa aatatcaaag
    10981 tacaaaaaga aaaacggtca agtggcgtgg atgtataaag gacacattgc caccgatcca
    11041 cgaactggtg aaaaaataaa tactacgaga agaggtttta gtagcaaaca agaagctcaa
    11101 aaagattatg acgagtacag acatcaaata ttatacggtg ttaaaaagaa agcacctgat
    11161 atgacatttg aagacctgta taatgaatgg ataactcatc agcgtaccag tgttaaagct
    11221 tcaacaatcg caatctcagt aagatacgca aataatcaaa tacttccagc atttggtaag
    11281 cttaaactat cgaatatctc tgtcccatat tgtcaaaaag tggtagatga atggcacagt
    11341 aaatatgaaa gttacgatta tatgagaaaa cagactgcac aaattttacg ctacggggtt
    11401 gcaatgcaat atattgacaa taatcctatg gaaaaaacac ttcttccacg aaaaaaagag
    11461 tatgagaaaa atcgtaaatt ttactcaaaa gaggagctta ataatttact ggatgctttt
    11521 aaagattttg gtaacatgaa acaatacgcc ttcttcagat tattggcata cacaggtatg
    11581 agaaagagtg aagtactcgc tttacagtgg aaagacatag acacgtttaa taaagaacta
    11641 catgtcaata aaactcttgc tgtagatgaa tttggtaaag ttataattca atctccgaaa
    11701 acaagagctt ctagacgagt tatctcttta gatactgaga ctctttctat cctcaacaac
    11761 tggaaattac agcaaaaaga agagtattta aaacttggat acaatactag ctctaaagag
    11821 caacatgttt ttacaacagt taaaaacact ctatacattc caaacactgt taatgattgg
    11881 ttaagatata ttttgaaaaa atataacttg cctcgtatta cacctcatgg gtttagacat
    11941 acacatgcta gtttattatt agaagcagga gaatctgtca aggtagttca acaacgatta
    12001 gggcatgaaa atagtaaagt gacattagac atctatgcac atattacaaa taatgcacct
    12061 aagaaaacag gtcaagactt tgctgatatg atggctcatc agtaaaagag ctggtcaaaa
    12121 tctggtcatt tctagtatac aggcaaaaca aaaaagctag aacccttata tatcaagggt
    12181 tctagctcct ataacaggtt tacccaacac tgccttccat ctcataactg atcaatcgat
    12241 tgagttcgac tgcatattcc attggaagtt ctttggtgaa tggttcaaca aagcccatga
    12301 cgatcatttc agttgcttca gattcggtca ggcctcggct catcagatag tagagttgtt
    12361 cttctgagat ttttgagact ttggcttcgt gttctaaaga gacttggctg ttgtggatct
    12421 cattgaatgg gatcgtatcg gatttcgacc attcgtccat gatgatcgtg tcacattcga
    12481 tatgagagat cgaacccgca ctgtttttgg caaaggttac ttgtcctcga tagtttactt
    12541 ctccgccgtc ttttgaaatt gatttagaga cgatagagct agatgtattt ggtgcattgt
    12601 ggatcatttt tgcacccgta tcttggattt ggtttgctcc tgcaaaggcg atggagagca
    12661 tggtgcctcg tgcacctttt ccatctaagt aaacacttgg gtatttcatc gttgtttttg
    12721 cacctaggtt tccgtcgatc cattcaacgg ttgcgccttc gtatgctttt gctcgtttgg
    12781 ttactaagtt gtacacattg tctgaccagt tttggatcgt tgtgtaacgg caatacgcat
    12841 cttttcttgt aaagatttct acgatggcgg catgcaggct gttacttgaa taagtaggtg
    12901 ccgtacaacc ttcgacatag tggatgcttg ctccttcgtc aacaatgatc agcgtacgtt
    12961 cgaattgccc cgtgttttcc gcattgatcc ggaagtacgt ttgtaatgga acatcgactt
    13021 tgacggcttt tggcacgtaa ataaaggtcc cacctgacca tacggctgaa ttcagcgctg
    13081 ctaatttgtt gtcggttggc ggtacaagtt ttgaaaagta ctctttgaat aattcagggt
    13141 attctttcaa agcagagtca gtgtcagtaa agacgattcc taacttttcg aattcatctt
    13201 tcatattatg gtacacgact tctgattcat attgtgcgga agcaccggct agataagctc
    13261 gttcagcttc tggtatacca atcttttcaa atgtatcttt gattttttct ggtacgtctt
    13321 cccagtcacg agcgggtttg tcacttggtt tttggtaata tttgatggca tcgaaatcga
    13381 tgtctgaaag gtcggggccc catgtttgca ttggcatttt gttaaatgtt tctagagatt
    13441 tcagacggaa ttcaagcatc cattctggtt cgcctttgat tcttgacatc tcacggatca
    13501 cttcttctgt caagccttct cctgtactga aaaccggttc gacatcatcg tgaaacccga
    13561 atttatattc ttctaattct ggtacgccca tgttctcact cctcttcctt gtggtaatga
    13621 tggaaagctg cggtaccatc gttttccatt gcttttccta gtgctttcca ggcaagtgtc
    13681 gcacatttga tccgagcggg aaatttagct acaccaccaa gcatagcagc atcgcctaat
    13741 tggtcgggat ctttgacttc ttttccttgg acaagctgta gaaagtctac agttaaatgt
    13801 tctgcttctt ctattgattt tccgatgacg gcgtctgtca tcatcgaggc acttgccgta
    13861 ctgatggagc agccactgcc gtcaaaggcg atatttttga tgattccgtc ttttacatcg
    13921 acttgcaatt cgatcacatc tccacatgtg gggttgttca attcgatctt gcttgtcgag
    13981 tcagtcaagc ttccacgatg atgcggatgg gaagaatgat ccaagatcac ttgacggtat
    14041 aaattgtcta atttagatag tcccatgttg gaaaaactcc tttgttgcca tgattgcttc
    14101 aactaaacgg tcggcgtctt gcgttgtgtt gtagaaataa aagctcgcgc gagcagtcgc
    14161 tgatacttca aggtacttca atagcggttg cgcacaatga tgacctgctc tgacggctac
    14221 cccttccatg tctaaagctg tcgcgacatc atggggatgt aacccatcca aattgaatgc
    14281 aacgactcct gtccgaaaat tcgggtcttg tggaccataa acagtcaatc catcaattgc
    14341 ttgtaatttt ggcaacacat aagcggctag ttcttcttca tatcgatgga tattgtccat
    14401 accgagtttt tgcaaataat cgattgcatg gcctaatgcg atagcgccgg cgatatttgg
    14461 tgtccctgct tcgaatttcc aaggtagttc tttccatgta ctgtcgtata attcgacaaa
    14521 atcgatcatt tcgccaccaa attcaacagg ttccatttct tctaaccatt ttcgtttacc
    14581 atataaaaca ccaatacctg ttggtccgca cattttgtgt ccgctgaaag caaagaaatc
    14641 acagtctagt gcttggacat caatggccat gtgcggggca gattgagcac catctactac
    14701 gatgaccccg ccgttttcat ggacaagttc agctaattct tgaacaggat tgaagacacc
    14761 taagacattg gatacatgag caatggagac gattttggtt ttgtccgtaa tctttttcgc
    14821 tgcatcctcc atatccagga agccttcttc tgttagttcg atatatttca agacagcacc
    14881 tgttcgtttt gccaactgct gccacgggat aacattagaa tgatgttcca tgtaagagag
    14941 cactatctcg tctcctgctt tgatatagcg gtcgccgaag gttctggcta cccaattcaa
    15001 gcttgtcgtc gtaccacgag taaacagcac ttctgctgtt tcttttgcat ggatgaacgc
    15061 ccgtactttt tctcttgctt gttcataatc atgggtcgcg cgttctgcta acgtatggac
    15121 accgcgatga acattggcgt tatcgtgttc gtaatagtac gctaatgcct ggagtacttg
    15181 ttttggcttt tgggtcgtcg ctgcattatc cagataaact aaaggttcat cattgacgtc
    15241 ttgaaataag ataggaaaat cattgcgtag ctttttagca tcgatcatcc attcaacttc
    15301 ccttcgatta cttcgactaa ttctttccga acttgttcta cagggattgc tgttaagaca
    15361 gaaccgagga atcctcgaat cactaagcgc tctgcttctt ctttatgcaa tcctctactc
    15421 atcagatagt acatttcttc tggatcaact cgtcctacac tggcagcatg tccagcagtt
    15481 acttcgtttt catcgatcaa aagaatagga ttcgcatcac cacgtgcttt atcagaaagc
    15541 atcaagacac gactttcttg ttgggcatct gcaccttttg cccctttcaa gatgtggccg
    15601 ataccattaa aggtcaatgt tcctttttct cggatcactc cgtgttgcaa aatatgcccg
    15661 atcgtatgag gggctttatt ggttactcgt gtatcgattc cttggatttg tctgcccgag
    15721 ctgattgcca cgattttgac ttctgcgtgt gcaccttctc ctgctaaatc tgagtcaaag
    15781 tcagcgatca catgaccgtc attcatgaca ccaattgccc agtcgacaga agcatctcgt
    15841 aaaatgtgcc cacggcgatt catataagaa gtaatatttt ctcctaattg atctacggct
    15901 gaatatttga tttttgaacc agctttagcg atgacttcca cgatgatatt acctgatgat
    15961 tttgctactt gttctttggt tgtctggaaa cgttccagat aagaaaattc actatgttca
    16021 tctgccacga tcaacacatg cttgaagaag tgctcatcgg atgcgccatc ttgaatgaat
    16081 aaggactcga gtggttcatc gatcacgaca tttttaggca catacaggaa aataccgctg
    16141 ttcataaaag ctacatggag tgcagtcaat tggtcttcgt tcatgtttac tgcttttttc
    16201 atataatatt cttggactag ttctggatat tcgatcattg cagtgaacaa gtcggtaaaa
    16261 atgactcctt tttctgctaa ttccacaggc agttgttcaa aaatcgtcca tgaaccttgt
    16321 tggacaagca caggattgtc tttcattgca tcaaatgccg gaatcgtgcc tgtttcactg
    16381 atggtttgtg tttctttcac atcaaataac gaccagcgat ggaatttcac ccgatcgata
    16441 tgcggcaatg gcagttcatc tgctttcgct agtgcgtctt gacggaaggt tctcatccaa
    16501 tcaggttcgc ctttacttaa agaaaaatca tttaccgcgt caagataatc gagccagttt
    16561 ttttctttca ttcttgctcc cccttacgct tcttcttctt tatagtcgat tcctaattct
    16621 ttactgatac cagcgtagcc ttctgcttcc agacgtttag ctaggtcagc attccctgtc
    16681 atgactactc ggccttccat catgatgtga acgacatcag gtgtgatata gttcaataat
    16741 cgttgatagt gggtgatgat caatgcaccg aaattgtctc cgcgcatttc atttactcct
    16801 ttagcaacga ctttcaaagc atcgatatcc agacctgaat cgatttcgtc taaaattgca
    16861 aatgtaggtt ctaacatcaa caattgcagg atctcattcc gtttcttttc tccgccagaa
    16921 aaaccttcgt tcagataacg ttcagccatt tcttctggca tatttagtaa agccattttt
    16981 tcatctaact ttttcaaaaa tgccataaca gaaattttgt cgttttcatc acgtttcgca
    17041 ttgatcgctg cacgcatgaa ttccgcattc gtgatacctg gtatctcact tggatattgc
    17101 atagctaaaa ataagccaag tcgcgcacgt tcatctactt ccaagtctaa aacatttttc
    17161 ccgtcaaaca aaatttcgcc ttctgttact tcatagtttg gattgcccat gatagcggca
    17221 gataaggtag attttcctgt gccgttaggt cccatgatgg catggatttc tccggttttc
    17281 attgttaggt ttacgccttt aaggatttct ttttcctcga tcgacacatg tagatttttt
    17341 atttctaaga cagacatgca ttataccccc aaattctctt tcgttctcac tcattttaga
    17401 ctaattgaga atgaagcgca atgaccttcc ttactaaaat caaatatctt ttatttatta
    17461 ttaaacaatt ttgatttcta aattagaata ggtataaaaa agatgagaaa taagccatta
    17521 cagacttatt tctcatcttt tttgttccat accttatttt gcatcagcct ttactggttt
    17581 aacagagccg ccccatttgt ctgtgatcca ttcttggact tctttgcggt gaagcacttc
    17641 aatcagtttt ttgattttgt cactgtcttc atcaccttta cgagtcgcaa taatattcac
    17701 gtaaggagag ttgtcctttt caagcaatac ggcatcttct aatggattca accctgcgcc
    17761 gtaagcgaag tttgcgttga ttgcaactag gtcaccttca tcattttggt acgtagaagc
    17821 taaaagtgat gggtcgatcg tatggttgaa cttcaagttc ttagggtttt cttcaatatc
    17881 atcgaatgtt gctgtttcca gatctactcc atctttgact ttgactaagc cagcatcttg
    17941 tagaattgtg atgatgcgtc cccagtctga ttcagaattt gaagtaatga ttgttgcacc
    18001 atcttttaat tcggaaacat ctttgacttt tttagagtac aagcccattg gttcgatgtg
    18061 gacagcaccg gcattgacaa agtcatatcc tttttctttg atttctttgt tcaaataagg
    18121 aatatgctgg aagtaattgg catcaatgtc gccttcttcc aaagattggt tcggtaatac
    18181 atagtcatcg aatttgacga tttcaagatc gattccttca tctttcaata atggtttcac
    18241 atgttccaaa atctctgcgt gcggtgtcgg tgaagcgccg actttcaatg tcgtgtcgtc
    18301 tgctttattt gctgaatctt tgctgtcgct gttgttaccg catgctgcta atcctacagt
    18361 cagcaaaagc gctgctgcaa atccaaaaat tttctttttc atatcctaca tccccctata
    18421 tcctattatc ttttgtctgt tttcttagtt aagaaatcac cgattccttg aacgataaat
    18481 acgattagta aaataattaa agtcgcaacg aatgtcactg ttagattacc acgctggtag
    18541 ccttcctgcc aagcaagacc tccaagtcca cctgcgccga tcgcgccggc catcgcagta
    18601 aatccgatca tcgagatcgt tgtaactgtt aatccagaaa tcaatgcggg aatactttca
    18661 ggtatcaaga ccttccagat gatttcccag taacttgctc ccattgcatc tgctgcttcc
    18721 agtacaccag ggctgacttc acgaaaagca atctctacta gacgggcata aaatggtgct
    18781 gcggataaaa caagagctgg aatggctgct tgcgcaccta gcatagtgcc tacgattgct
    18841 ttagtaaatg gaatgatcaa aaccatcagg atcataaatg gcgttgaccg gaaaatatta
    18901 ctgatgatcg atacgattcc ataaatgatt cgagcatacg gtttgttttt gcgtccaata
    18961 gagtaaagga tcaatcccaa aactaatccg atgatcgtca ctaagaccat tgagattaat
    19021 gtcatatata gtgtatcaag gatagcctga ttcatcgttt ccgggttgat ttgtttaaaa
    19081 tctagatatg tttctgaaaa gcttttatcc acgatggatc acctcaattc ctactccttg
    19141 ttctttgatg aacacttctg cttgatctaa ttctttggtt tcaccagtca tcaagacagt
    19201 caatgaacca aaggaacctt ctttcacttg tttgatcttt ccatacacaa cgttgacatc
    19261 gatgttatag cgtcgaatcg cttgggaaat gactggctcg ttggcatttc tttcgctaaa
    19321 ggtcaaagtt gccaaacggc cgcttggatt ttctttgatg agttcagcta atagttctgt
    19381 cgaatcagct tctggttcga tgtcttgttg tacaaagcgt ttggttactt cttttttcgg
    19441 ttgtctgaag acagtcaata cgtctccttc ttctacgact ttccccaact ccatcacagc
    19501 gactttgttg cagattttgc ggatcacatt catttcatgt gtgatcaaga cgatggtcaa
    19561 attcaatttc ttattgatcg ctaacaataa gtctaatact tcttctgttg tttgcgggtc
    19621 caatgcgcta gtagcctcat cacaaagcag gatctcagga tcatttgcca atgctcgtgc
    19681 gataccgacg cgttgtttct gtccgccgga aagttgtgaa ggatatgctt gtccgcgtcc
    19741 ttctaacccg actaaacgca acagttcttc tgcccgttct ttacgtttgc tctttggtac
    19801 gccagctaat tccagcggca gctggatatt ttcaagtaca gttcttgacc ataacaggtt
    19861 gaaatgctgg aagatcatac cgattttctt tcggaatgta cgtaattctt tgttttttaa
    19921 ttttgtaata tcttgatcgt ttacgatcac ttcgccgctc gtgggaagct caagtccatt
    19981 caggagacgg actaatgtac tttttcctgc gccagaatag ccgacgattc cataaatatc
    20041 ccctttttca acgtttagtg aaacgtcatt gacggcttgg atgtttccgt ttttcccatt
    20101 gaatgtcttt ttgacattcg ttagttggat taatgacatg ccattcacat ctttctctta
    20161 tgatttttca aacaaaaaag ccttcgccct cttgccttaa gcaaaaggac gaaagcttga
    20221 acttccgtgt taccaccttg atttaccgtt acttcgcaat aacagcctct tccagtactt
    20281 ggacttagct tctttccgta tactgtcgcg ctgtaacggg cactcccgta gtaagtttgc
    20341 ttacgctcgc ttactctgct ctaagaccat cttccatctc attttcgtta cccattttca
    20401 gcaaacaggg ctctcttcaa acgtccgtga catgtactct tcttttcaaa gctttctcgt
    20461 ttcatatagg attgcttatg attatagcaa ctggaagaat cagtgtcaac ttttttcaag
    20521 tgattttctg attttataat tcgtcaaatt cttttggatc aacatctttg aaacttaaga
    20581 tccacgcatt catttcatct gaatcattca agatattgat atcttcaccg attttatcat
    20641 taacagaaga aactgtaccc gttagcgggc tggcaaattc attgaccgct ttttccgctt
    20701 caacttcgat cagaggttca tcttttttta gcgattgtcc tgcttttggc agattggcaa
    20761 acgtgatgtc tccaagttct tcttgcgctt catttgtcaa accaacacag tattcttttc
    20821 cattaaataa gatccaaagg ttgtcctttt tcttcaaatg cttgcctgcc attttatatc
    20881 agttctcctt cataaatttt ttcattgaat ccaattgcaa caagctggtc ctctcgtaag
    20941 atcagtggtc gcttgatcag cataccatct gtcgataaga cttggctcgc ttttgaagcc
    21001 gtaaactcgc ctacttgatc tttcagtcca agttcccgat atttcatccc gcttgtgttg
    21061 aacaggcgac ggattggtac ttgactcgtt tctagccaag tctcaatcgt tgaggccttc
    21121 ggtacttctt tgatcatatc aacggtttga taactaacgc catgctggtc taaccacgct
    21181 ttggcttttt tgcatgttga acatttcggg taccagtaaa aggtgtacaa ttttcttacc
    21241 tccttgaaga tcttgcaggt gcttcatttt ggtatctcat cgataatatc aacccgatac
    21301 cgatcatgtt ccctaaaatc gaggatcctc cctgactaat gaaaggcaat gggatccctg
    21361 ttaaaggaag taatccgata tttgctccaa tattctcaaa gacatggaac agcagcatca
    21421 tgatcaaacc gcttgcgata taggcgtaaa attcattatt cgtatcgaaa cacacccgaa
    21481 tcatacgata gattaaaata aaatacaaga aaatgacgaa tgcaccaccg ataaagccga
    21541 agttttcacc gataacggta aaaatcatat cggactcacg aacaggcaca tagatattgc
    21601 ttacgttgaa tcctttacca aacaagccac cggtaccaat tgccaaaagt ccttgggccg
    21661 gctgataact cattcctgac gtatcatgga aagggtccag ccatgaatca atacgtgcaa
    21721 actgataaga tttgaatcca actttataaa gcaagtctct tccgccttca gtcgtaacca
    21781 agaagatcgt tcctgctcca acgactactg ccaagattac tacaggtgcg atgatgcgcc
    21841 aagagattcc agacatcagg aaaatcccgc caaagatcgc aagaaagaca agcatcgttc
    21901 caaagtcatc ttgggccatg atcaatgcta aaaccggtat tgtcacgatc aagagcttcc
    21961 caatcagcca aaagtccgat ttcatcgtcc gttcttttac ttgcgtatta tgtttagtta
    22021 cgatcagtgc catcatcaag atgtacgcaa tcttcatcaa ctctgctggc tggaaagtaa
    22081 acgcaccgaa tcgaaaccag tttttagacc ctgttgaagc agctaaattc tgatcataga
    22141 atttgagcaa gagtcccatt accactaacc ccaaaccata aaggtaagga gtcagtttcc
    22201 agatccattt ggaattcaaa tgcatgatga caacgattgc tgccgctcca actaaatacc
    22261 agacaacttg catccctacg cctttcaaga ctgctggatt gttcggatca tggctaagtg
    22321 ctacatataa tgagagcaga ccaatcaaac ataacagaaa gactggtaaa atcacgccat
    22381 aatcaatacg attatcgttc gaagctttta attttttttc catgtttcat cccttttctt
    22441 aaaaaccata ctgctgatta ttatagcggt ccagttagat aatggaaagt ctttccatag
    22501 acatttggga aaaattaact ttccgtttgt gcagttgctt gagcaaatgc agtacaaaag
    22561 cctactgctt atctttagtg tatcatagaa ctttttttga tacatgcaat tatctaccag
    22621 ctgagattct cctcgctgtg agaagtttct gtgctttcat tgtatcgaag gatctcttcg
    22681 gcaatctcta gcagctgatc agaaaccaga ttggttcgtt ggatgaaata aaaatatcga
    22741 tggaaattca acggagtcat tggcacattt tctggaacag agcgcttaga caaaatcgag
    22801 cgaccgattc cttccttcag caacgcaaca attacatcgt tacttttcac gatcatcttc
    22861 ttatcttgga tattcatctc ttcaaaataa cgctgcgtgt aatgaaaaac accagagttt
    22921 gcttctctta caagccacaa tttgcttttt gggtccccag cgatgactag ctggtcttcc
    22981 atcaaagagt atctttgtac gccggaagtc tctaatggct tttcgataaa accaaaatcg
    23041 gattcatgtt tcgctacttg ttctaatgat tcatgagaat tcgtgatttc gatctcaaaa
    23101 tgcactttag gaaacctttt gatcagatgt ttcatcaact gaggcaaaag atagacagca
    23161 aaggtgtggg aagcaacgat ccgacatact tcttcttcat tttgttcatt gagcaatttt
    23221 tgctgagttt cttcccattc ttctaacaaa accagtgtcc gttcatataa taagtcggct
    23281 tgcttagttg gaagcatctc ttttcttccg ttacgttgaa aaagatcgat attcaattcc
    23341 cgctccaact gtttgatttg attcgagact gctggttgag ataaaaacag tacttctgct
    23401 gctctggaaa aatttcttgt ttcatatact gctcgaaaag tcgttaataa cttaaacatg
    23461 gcaccacttc cataactttt ta
//
LOCUS       DACDIE010000041        23072 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.041, whole genome shotgun sequence.
ACCESSION   DACDIE010000041 DACDIE010000000
VERSION     DACDIE010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23072
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.041"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..400)
                     /locus_tag="IV250_001769"
     CDS             complement(<1..400)
                     /locus_tag="IV250_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769052.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLN"
     gene            complement(393..794)
                     /locus_tag="IV250_001770"
     CDS             complement(393..794)
                     /locus_tag="IV250_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769053.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            complement(784..1137)
                     /locus_tag="IV250_001771"
     CDS             complement(784..1137)
                     /locus_tag="IV250_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAP8769054.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            complement(1127..1438)
                     /locus_tag="IV250_001772"
     CDS             complement(1127..1438)
                     /locus_tag="IV250_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769055.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            complement(1435..2262)
                     /locus_tag="IV250_001773"
     CDS             complement(1435..2262)
                     /locus_tag="IV250_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769056.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLSFVNKKKCSYAYVKAIKAVVR"
     gene            complement(2272..3432)
                     /locus_tag="IV250_001774"
     CDS             complement(2272..3432)
                     /locus_tag="IV250_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP8769057.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            complement(3432..4118)
                     /locus_tag="IV250_001775"
     CDS             complement(3432..4118)
                     /locus_tag="IV250_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAP8769058.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            complement(4081..5259)
                     /locus_tag="IV250_001776"
     CDS             complement(4081..5259)
                     /locus_tag="IV250_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP8769059.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            complement(5279..6973)
                     /locus_tag="IV250_001777"
     CDS             complement(5279..6973)
                     /locus_tag="IV250_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP8769060.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            complement(6951..7265)
                     /locus_tag="IV250_001778"
     CDS             complement(6951..7265)
                     /locus_tag="IV250_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HAP8769061.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            complement(7368..7649)
                     /locus_tag="IV250_001779"
     CDS             complement(7368..7649)
                     /locus_tag="IV250_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769062.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            complement(7654..7998)
                     /locus_tag="IV250_001780"
     CDS             complement(7654..7998)
                     /locus_tag="IV250_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP8769063.1"
                     /translation="MTEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            complement(9185..9652)
                     /locus_tag="IV250_001781"
     CDS             complement(9185..9652)
                     /locus_tag="IV250_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HAP8769064.1"
                     /translation="MVLFDVKKYETPEAKDVDMDRTKHNVGVFLSAYLSARCRIGQPR
                     EPKVTASYSLVPSSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKLESNKIIIQFASSLELVAFK"
     gene            complement(10244..10531)
                     /locus_tag="IV250_001782"
     CDS             complement(10244..10531)
                     /locus_tag="IV250_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769065.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKEPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAVIRIENTMDCDKELAKSKANIAVVRLVDMKAI"
     gene            complement(10531..10725)
                     /locus_tag="IV250_001783"
     CDS             complement(10531..10725)
                     /locus_tag="IV250_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769066.1"
                     /translation="MKAYTIKFYGNKTFVYILSGLRLDFRGYSKDDLLESLYGYGYLN
                     EDELISLEKFTNAWTIEEEA"
     gene            complement(10728..11114)
                     /locus_tag="IV250_001784"
     CDS             complement(10728..11114)
                     /locus_tag="IV250_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769067.1"
                     /translation="MKSLQQLISEKESELQNLEDSLGLGFPIVEQAKMTQISHLRLES
                     EDLRQIEKSIQLNDNQQIVFDFLKNEFKRNDLQYTLWSFTEDVYERLEIGVSSLPYVK
                     AWGKLKEKQQFEVLTAFAQWGLEQEE"
     gene            complement(11111..11650)
                     /locus_tag="IV250_001785"
     CDS             complement(11111..11650)
                     /locus_tag="IV250_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAP8769068.1"
                     /translation="MNKQELIDKLASLVGSIEDFKGISTFHDGKYAGLEHALELIKQL
                     DEPQKPFVPKFVADYIEEYRGGGATLYEMMDSSEDEVYTWLFGNPRIKTENERQLLFA
                     QAYAVGYEVEKEPLYEVIIGNLFLVKKFNNRNDSCFVNKFELCWWEKSAYQLTEPEIK
                     AIDERYWPFAVPVEEVAEG"
     gene            complement(11643..11963)
                     /locus_tag="IV250_001786"
     CDS             complement(11643..11963)
                     /locus_tag="IV250_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017547333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HAP8769069.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILDEYME
                     DSDE"
     gene            complement(11963..12262)
                     /locus_tag="IV250_001787"
     CDS             complement(11963..12262)
                     /locus_tag="IV250_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769070.1"
                     /translation="MDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            complement(12265..12426)
                     /locus_tag="IV250_001788"
     CDS             complement(12265..12426)
                     /locus_tag="IV250_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP8769071.1"
                     /translation="MTTEEVIQMRIRSIQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEG"
     gene            complement(12423..12725)
                     /locus_tag="IV250_001789"
     CDS             complement(12423..12725)
                     /locus_tag="IV250_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769072.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            complement(12887..13156)
                     /locus_tag="IV250_001790"
     CDS             complement(12887..13156)
                     /locus_tag="IV250_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769073.1"
                     /translation="MKIDYLELINEIAKYKTGEEIEILRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            complement(13168..14019)
                     /locus_tag="IV250_001791"
     CDS             complement(13168..14019)
                     /locus_tag="IV250_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP8769074.1"
                     /translation="MADNKRYYYLKLKENFFDSDEMVLLESMPDGYIYSNILLKLYLR
                     SLKHEGKLMFNDRIPFNSTMLATITRHSVGVVEKAVQIFRDLQLIEVLDNGAIYMSDI
                     QSFIGKSSTEADRKRKYRKEIEEAKQNLITSGQMSDKCPDKTTPELEIELEKDIELEK
                     DIENVTPSKKSKAKPVRHKYGEYKNVLLSDEQMEKLKIEFPNDYQERIERLSEYCESS
                     GKTYKNYLATIRSWARKEKSESKNASSGYKRTGRREKLPEWAIDQEAYLKKKALERAN
                     RQSKAPF"
     gene            complement(14026..14712)
                     /locus_tag="IV250_001792"
     CDS             complement(14026..14712)
                     /locus_tag="IV250_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769075.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDIGATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
     gene            complement(14718..15389)
                     /locus_tag="IV250_001793"
     CDS             complement(14718..15389)
                     /locus_tag="IV250_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAP8769076.1"
                     /translation="MSENKGTTTFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            complement(15382..15723)
                     /locus_tag="IV250_001794"
     CDS             complement(15382..15723)
                     /locus_tag="IV250_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769077.1"
                     /translation="MEKELSTLDQYLTDPSWGKSNIKETNNRKIRRNLLTDEELACDQ
                     DDLGNFVSIWDHVYLIHLSRKSKKPEYIYVIEDGLIDALEEYDRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            complement(15901..16608)
                     /locus_tag="IV250_001795"
     CDS             complement(15901..16608)
                     /locus_tag="IV250_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAP8769078.1"
                     /translation="MQELVILKNKEAVTTSLQVAETFRKEHRAVLKAIDDLKDGLAQK
                     YADLFWEDVYIHPQNKQAYRIVYMNRDGFTLAVLGFNNTQKVLEFKLKYIEAFNEMEK
                     AIRKNTAFDTIEDMMIYQLEEMKDVKKDVSMLKNTMRISGQQEFEIKQKGNTKVMEVL
                     GGKESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRM
                     EIDQLNRQQRLFGDENE"
     gene            16659..17441
                     /locus_tag="IV250_001796"
     CDS             16659..17441
                     /locus_tag="IV250_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769079.1"
                     /translation="MDISDTPYYQKLIASSKLIGETIAPSLDVVNAMQPAIEQAKKLI
                     AIQDTFIASQAILNDSLIQIVEKRNKSIANMIPPSLFAIQKQLESPSFNIAKKMAATM
                     PKPYFSDISKITERFAVDMSKYDFRIGVATQELEKAFSSEPEVSTKNLLFKDTSNPSD
                     EFEAKFFDILNEQRKVLDDSIKLFEEINTAKNKDITNEPSEKQQASPAFYKDKMWYLE
                     QTGAALIGLVVTEIVNITIGVDPNNTISLALFLRYLLQFLIN"
     gene            complement(17623..17943)
                     /locus_tag="IV250_001797"
     CDS             complement(17623..17943)
                     /locus_tag="IV250_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HAP8769080.1"
                     /translation="MQYLEAKIPVPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRR"
     gene            complement(17959..18162)
                     /locus_tag="IV250_001798"
     CDS             complement(17959..18162)
                     /locus_tag="IV250_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8769081.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            complement(18177..18950)
                     /locus_tag="IV250_001799"
     CDS             complement(18177..18950)
                     /locus_tag="IV250_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAP8769082.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVADVLGYSNPQKAIR
                     DHVDLEDKTQNDSFTVNGTAVVLINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIRK
                     HGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSST
                     DSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPKA
                     DGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            19028..19336
                     /locus_tag="IV250_001800"
     CDS             19028..19336
                     /locus_tag="IV250_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769083.1"
                     /translation="MKPEFVITMLDGEKIKIFADTLVVGYDDASNIEEKDGMFYSSQI
                     YVNTLQGDIGVSTLATSRPLVGLIGFLSKATFFSLGDDIDSDETVIYKTSAVKSIGFK
                     "
     gene            complement(19307..19564)
                     /locus_tag="IV250_001801"
     CDS             complement(19307..19564)
                     /locus_tag="IV250_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HAP8769084.1"
                     /translation="MNKEMSQTIKVQKMIDDLTHGIDSQADKIIKELQGQKVKDAKML
                     LKTINFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            20296..20616
                     /locus_tag="IV250_001802"
     CDS             20296..20616
                     /locus_tag="IV250_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8769085.1"
                     /translation="MITFERIKELAKKQGKSLNKVEEDLGYGKNVLYRLKNSNPSTER
                     LQEIADYFDVSVDYLLGREEKETPKHVDLSENDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            20634..21065
                     /locus_tag="IV250_001803"
     CDS             20634..21065
                     /locus_tag="IV250_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP8769086.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            21192..21776
                     /locus_tag="IV250_001804"
     CDS             21192..21776
                     /locus_tag="IV250_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HAP8769087.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLAVILIIIIGSALNGGS
                     DSNKASDNGGEKVTKSSTSASSSKEEKSDTFYKIGDTVKVGDAEYTLNSVELTDERNQ
                     FEENQPAQVVKITYTVKNDGDSDIPVGTDVEVYGPDDKKSETYANENTMGSVAPGKQM
                     DVTAHFTLNQKGEIEIHFSPLVSFEKAAIFKATV"
     gene            21890..23029
                     /locus_tag="IV250_001805"
     CDS             21890..23029
                     /locus_tag="IV250_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8769088.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEVK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
BASE COUNT         6715 a         4575 c         3567 g         8215 t
ORIGIN      
        1 tatttaattt atcgaattga cgaatcagaa gttctgtaat tggatcaagt gcaagaccta
       61 atcctcttct cataaattcg tccggctgat ttcttttaga agtacctatc cccaaatcag
      121 gatattttaa atactcaaat tttcttgtag gtctaatgat gaaacccaaa ttaatgtatt
      181 gagtcttaag tggacgacta ttttttgcgt gttggtgccc tcttcttaaa tctgcttcag
      241 aaacaggaat tttttctgta atcctatcca ctgcaatagc cgaacctttt gatttcaatg
      301 cttcgttaat cagtcgttcg ctctcgcttg aatagcgttc catccgcaca agaagttcat
      361 catgtccatt tatttttagc tcccaactat ttttagccat gacaatcact cttcaataat
      421 cgtctaaacg taaataccaa ttgatcgata tagcgatctt ggttctctag ttttaaatga
      481 ttgggatcca ttctctgaaa acgaatcgaa cgattttgaa tcaatgaaat aatatctagt
      541 gaatctcctg ttaaatcttc tctattttct gaatagaaag ttagatatag attttgaccc
      601 acgctatatt ttggctcagt gatcatttct atttctcctg tttcgagaat gaagtaatta
      661 aaatcatcag gtagctcatc ctcgcctacg gagtcttgaa agagtttgag gccaaaatgc
      721 tcttctaagg aagttttgat agcagaaatt tgcttattta aacgttcttt ttctttagaa
      781 ttatcaatca ccatattcac ccacactttc aagataaaaa tagatataaa aattatcgta
      841 atcggcatag ataacgttgt aacgcatact atcgattacg ataaaatatt gatctttatt
      901 aaatttcttg gcgattggat gaaatggagt ctttactttc ttagttaatt tcgatcccat
      961 cgcatccata gctgttatat cactatctct catggaaaga tttctaaatt ttaaagaagt
     1021 gatttctgta tcttctacac caatcttttt tcctagttca tttcttttgg tagtttgcgt
     1081 caaaatcttt aaccaaccat cgttgaatgt ttcttcgagt ctacgattat tcgccattca
     1141 catcacctgc aatatattct tgtagcgcat aatgttgaat gaaacctaat aactcactag
     1201 cgaaattttg ttcaaactca tctaaagcac gattccagtc gtatctacat ctttcgatta
     1261 gcaatccgta ttctaagctt tcaggagaaa aagaaagtgt tgtactcact ttactttgaa
     1321 gataaacagc atttttagct atcatctttt taattgactc atcttcttcg ttccaggtaa
     1381 cgtaaatatt atccttcaca gctattagca attcttcagt cacttgttca ggcgtcatct
     1441 aaccaccgcc ttaattgctt taacatatgc gtaagagcat tttttcttgt ttacaaatga
     1501 taaatcttca tcgtttgttg ttactccagc attgtgtaag atttttactt ctttaacttt
     1561 ttctattgga tcagtagcaa aacaaaagtc taattcctcg tgaactttag gaccaatatt
     1621 gaaatacatc atgttccaaa gctgtgccca catctcggct gtccagattt gtatatttgt
     1681 tttttgccct ctaaggtagc gatatagccg attagaatcc agataaacct ttttccaata
     1741 attcgcttta gggcggttaa taacccactg tgcgcctcct gaattagtgt ttatagtttc
     1801 caaagattct actgtaacat ttacaatgtt tgccatatct tttagaatat tttctccgtt
     1861 ttcacagctt ctaatataat caagacttag ataactacag cagtcgctac aataccaaac
     1921 atcatcttta gaaggcaatt tgcgcaaatt aattctttta ttgaaaatga catccgaatc
     1981 gatatagaaa tatcggtcgt cctcacgcga atgatcttct tctaaatatt tccaccataa
     2041 atatggttta atcgaaggaa tatactcttt gtcgtcccgc agatcatcgt acacatgaac
     2101 ttcaacacca tattccttct caaaaaaaat aggaatctga tcatcgtgtc tgctgaaaag
     2161 caatacgata tctttgattc ctagtttctt cagattagtt aaacaaactt caagctccca
     2221 tttaaaccga ttgattgccg gctgacaaag aatatacttc attctgatca cctacgcttg
     2281 tgttgtagtt gttgtggttg ttggttttgt agttgtagta gtagttccca aagcgctaat
     2341 atctaataca atgaaactat cgttacgttt aggttgaccg tttgcatatt gtttagctag
     2401 ataaatgcgt tcgtcttcaa caaaatggta ttcatctgaa gcttcaattt ttagtgtaga
     2461 tcctacaccc atgaagtaat ctgaggctac cccaataact gcttttcctt ctggcacagc
     2521 cgttgactgc aaatctgaaa ctggtactgg caatacttgt acgtattctc cattagcagt
     2581 tagtacagtc ttagctggga atactttaga ccagtaatca gttggattca caattaggac
     2641 cacatcagaa ggattcacat tacgataaat cggatcattc acaccttcga tattgaattt
     2701 tgatagtcgc gccatcaaac cgcccatagt tacagcatct aaagctgtaa taggttctgc
     2761 ttttttttca gcatattctc cgctagtttg tttgctcatg tcacgcatca ttccgactgg
     2821 catatcttta ccagtaccat caacaattgc ttgttctaat gcaattctca atgattctac
     2881 taaaacagta cggacataac gatctaacca tactggacct aaatcaagca ttgccttaca
     2941 tacaggaata taacctgata gcttgaactg cttcatgtta attacatcaa agccattatc
     3001 taaaactttt ttaacagctt cgcaaagttt accccaccat gctggattga ctccacgtga
     3061 cacaatccat tctgttacac cagttgtgtt aacaaaagta attttttgca atagtggatg
     3121 agattgttct aaatcttcaa atacacgttc aaatacagta gctggcacta attcttcgac
     3181 ccctgcaaaa ccttcgtttt tcactacttc gttatagaat tttgtttctt gtgtagttaa
     3241 tacacgctga ccacggttca ttaatactaa ttgatcttga ttttttgctg ttgcttcttc
     3301 taaaatttta tcctgaattt ccttagataa gcttaccata gctgcgctaa aagattcttc
     3361 gttaccatct ttaaaagctt tcatcaattg gtcgcttgca gctgttacac cttttaaatt
     3421 tttaactgtc attattttac atctccttgt ccaaatgttt tatttaatgc tgctgtaaat
     3481 gcagcaattt tttctgctct tttttcttta acgtcattca aaatttcttc aacgctttgt
     3541 tcttttttag cttcagtacc tgagctattt tctgcatcga taatttcatc gaccaatcca
     3601 taactcaaag ctgtttctgc atccataaac gattcttttt caagaagttc ttgcaatgct
     3661 tcatctgtgc cattgaatcg tgttttatat gaagccttta ccgatttatc aattgattcc
     3721 agttggtcag caatcgtacg gaagtcatcg acatttcctt ctccgtatgt ggaagcgcgg
     3781 tgaatcatca attgtgcatt gttgtagatt tttatagtat cgccagccat tgcgataatt
     3841 gaagcagcac tagcggctaa gccgttaatc acaacgttaa cttttgcttt atttgactta
     3901 agtaagttcc caatagcaat cccttgaaat acgtctccac cgtttgaatt aattactact
     3961 tcaatttctt cttgatcacc tagactatcc aaaatatttt tgattccctt gtcagtattc
     4021 ccttcaaaga accaactaga accaataaat ccctgaataa aaatttgcgg tactgcgcct
     4081 tcattcttta ctgctagaaa tgttttcatt gtcgtcattc gcctcacctc ctttcgatac
     4141 ttgttgattg tttttagtta taaatatttc atctgccatc gccttatcag agcgatcatt
     4201 tccaacgcgc tctcttcctt cgttgattgt aaatactcca tttctaatgc ctacatcaat
     4261 agcgtcaacc aaatctttga agctagtaat cttgatcata gttgtatcca cacgtacaaa
     4321 attccctgac aagtattctt ctacttcata gagactagcg ttaaacgcat cctgaataag
     4381 ttcagcaatc ggtatgattt cgaacattaa aaaagcgtcc acttgatccg ataacccact
     4441 catgtctccc tttagtaggt ttttcggaac gtgaaacgct gctgctgtca tctcaaagat
     4501 gtcgtctatt aagtttttta tatctcttga attgctttgg aagtttccgc tgaaatcttc
     4561 taatgtgtac tcattttgta attgaaatac cgcacctgca ttatcagctt ccataaaagc
     4621 cttaaattgt gatgtcatca ttttattgat ttgatcttgt gttgtattgt cttgcggtcg
     4681 gaataaattc cctttcagta cgtatctacg agcgttagag cgcttgtaaa cattcatggc
     4741 actagaaatg agtttcccat acgcttgata atacgcatcg actagttgcc taatttgttg
     4801 atctgcgtat tttatataga taacatcact ttctagaaat tctctatcaa ggactatgtt
     4861 gttaatttgc acttgagaaa acacatcatc tttcaatgca tattctgtga catcccaact
     4921 atccgcaata aatatttcgc tagaattatt agacggagaa acgatcaata cttcattgta
     4981 gaatattaat ctcctgatca gtttttttct aaattctgtt gcattatttt tcttattagg
     5041 agctacattc agcctatagt aaagatcatt ctttttattt tttccatctt catatgactt
     5101 gaattccgct ttactcatcg catttgcaat caaatcaata caagtttcaa tcgcaaattt
     5161 tcgatacaca aaatcaactt gcaatttaca aaagtattct tctaaaggaa ccgttgcttt
     5221 ttttgtgaag tatcctaccg ccttttgaaa aatccccact ttctcacctc ctttcaagtt
     5281 agaatactag aggagtaaat ccagttcctg tattttctac tgagctattt gtgactgtta
     5341 caggagcaga atcataaata tcatctaaaa aattcaaacc atgaaggaat gaaaaaaagc
     5401 catccgtttt tctagtttca ggttctattt tttcatagcg tatatttcca ttagaaatat
     5461 gctcttcata tacattcatg caataccaac gcataatcgc atcgtcacca aaaaataaac
     5521 gttgattaat aaaaaggtca tcaaccagat cttttaacat accatgtgta acagatccgc
     5581 ttcgaacaat ttccacagta aaacctgctt cttctaaagc gggcttcaat atttttgcac
     5641 ggtacatatc catagcgatt tttttaatat aatatttatt actcatttca agaaaccaac
     5701 ctacaatata atcagcttct atattttttc catgaacgat ctgtgatttt ccttgatcta
     5761 tagaaatatc tataacctct cgtttgatgt tttgtaatcg aagggctgat tcgtggataa
     5821 aagtatgttg tgtaaaataa acatctttat cgtattttcc tagcaaccca acgctggcaa
     5881 aatctcgtct atcagcaaaa tcgactgttc ctatcacttc atccattttt tcaggaaatt
     5941 ctttttcttt cgtatgcaga acatcatcat atgaagcaac agcaaatcgt gtatcttcca
     6001 taggtctgtt cattcgtttg gtcatgaacg taagtcttaa accagcatta cgttgcattt
     6061 gagagtattc ttgaaacatt ttccgtttta aatctgcatt gtaattaata gttggacaag
     6121 ctttttccca catgtcggga tcatcaactt cattatcgtt atccaagcga caaataaatg
     6181 gaaacaaact agaaaattct gctccatcct tgtcaattcc aagttctcca gaaagaatca
     6241 tttttgattc ttctataatg tcatcaagcg gaccaccacg aacatgacca ttagttgtat
     6301 cataaaattc tctataatct cgaattttac caccaccaga agtagccaca tttatcattg
     6361 aataatcttc attttcgtga atttcatcaa agcggtttgc acctggtcgc ttcccatctt
     6421 ttgttctagc atttgccgtg ttataacgaa gtttgctgtt tgtagcgata ttttgaataa
     6481 cttccttcgt agctttaaat acttttttat ctaaatcagg atgatcttta attactttaa
     6541 atacatcatc aaaactagtc tttgcttggc tttcattatt ggcatagata tcaatatcat
     6601 aatttttaat accgtgttta gcggttagta gaaagaagtt gttccaagaa gcaaaaccag
     6661 ttttaccatt accacgtccc attaatgaaa gatatctatt gaacactagt gttttatctt
     6721 ttttccatcg aacaccataa ataaaacatt gtagaaattt ttcccacgga attaattcga
     6781 atggaaagta ttgtgctggt atattgattg aatcctctac catctgctta tcgaagtaaa
     6841 tatcttctct agtaaagact ctttcttcta gataattttt tagcaataat tgctctttgc
     6901 ataccttgat agtgccttct tctatagctt tgaaccaatt ttcaatatgc ttataactca
     6961 ggaattgatt catttgcttc acctaccaat tcaggagtaa tggcaagttt atccaacatc
     7021 aatcccattt gtttgttgac agaaacaagc aacgctactg attcattctt tttaccattc
     7081 tccagtctaa tgccgttctc ggatatatct tcttccagtg atatcgccgt ttcccataaa
     7141 ctgatataac gatcaacatt atctaagaat ggctcaatat ttgttttctg actttccaat
     7201 tggcttatta aagagcggcg taatttttct ctgtagcgat tttgagacaa ttcgttttta
     7261 aacattttag ccctcctttc atgataaagt tcgaaaaaat ctcttttcct gacagccccc
     7321 tccgtttcat cacccccaaa aaatttgcga tttattttaa gggggggtta tctcaccatc
     7381 ggaatgaaag cttcagcgaa gtcaatgtaa taattaatct cttcaatgct atatccaaaa
     7441 acatttttta ttctttcaac gttattatct ttattcaacg cttctcttac ttgattcact
     7501 ttgtatttac tacaacagtt atctgataac agatccctaa tacctacata gcgaacgtat
     7561 atcaaacgtt taatcaatcc ttgtgtataa gatgaatact cttcaatctt ctctgtgtca
     7621 tactctctgc cattgtcatt gatgatcatg cacttaccac ctttcacttg catcgaagtt
     7681 agcgaagctt tctatcttct tctcttgttt atccaatgct gtaagatatc tgccatgaac
     7741 ttcattatga tgttcaacac ataaacaaat aagattatct aaatctaaag ctaagtcagg
     7801 tctatccttg acttccttta tatgatgaac gttctctact ctatgatact tacctagtct
     7861 tctacactct tggcattcat agtgatctcg tttcatcgct ttctccctaa gcctgcgcca
     7921 tttaggagac tgatagaact taaccaaacg atcttctctt atcaactgta atagccatct
     7981 atagaattcc tcagtcatga cccgtctcct ttcgcaatct tatttaatgc ttagctattc
     8041 ttttgccata caacggaata gcttcattgt tttcctttcg tttatatgta tcactcttta
     8101 ttggtcttct atactttcgt actatctcac cgttaccatt ttgcacagtg attacttcat
     8161 acttctgttc taagtattgt ggtctataca ttgttgttac ctcctttgtg caaaaataaa
     8221 aagaccactc aacgagtgat ctcatatgta atgcacaggc agggacgttt ccgatcctgt
     8281 gcttgagtca tttgacgatt catttgtacc aaatcccaaa actcaatcta acctaaccaa
     8341 ctatgtaata gcaacctaca ccgattccat cgattactat cgacctcgcc ttgctcgtgt
     8401 actttgagcg cccatttcca accctcagtt gctaatgaat actgaaacag aataccgtct
     8461 ctgttcatga ccaacgcgac agtacaaagc tttgtcgcaa gcaggttatc gtattcctaa
     8521 taaccccaac taatatttct ctcctaacct acacctgaga gtggcgcacg tctgcgctag
     8581 tattttatgc cttttaactg ctagtggcat cccgactagt cagccaacca atcggcatcc
     8641 gataagattg gttgtttccg taggttcctt aagtcactgg caatgaatcg aacattgcat
     8701 ggttgccgaa gcattgacct agcacgcatg cttagcgtct accctttccg ccacagtgac
     8761 actataaaat tattcttggc tgctactatt ttttattttg cccattttta aatccaatca
     8821 tatagacatt aagacagagc gcaaaaattg aaattattaa tggaatcatt tctcttcact
     8881 tacctttagt tatcgtgtga ataattaaaa aaacaataga cagcaacgga tgatagataa
     8941 taagaataat ttagaaggag ttgaaattca catccttatt cttaatattt ccgctgctgt
     9001 ctatcgaagc ttaattaaac gatgagggag atttcctccc ttacatttta ttttgtctca
     9061 gacctatcac taatctttcg acactatcat aatacaacat tgaataggta agtgattggt
     9121 ataaaaaggg tataaaatgg aaaccaaatg ggtaataaaa gggtataaaa aatgtaaaaa
     9181 ctggctactt gaaagcaacc agttctaacg atgaagcaaa ttgaatgata atcttgtttg
     9241 attctagctt gacagattct tcactcgtgt tatttctttg agcagttaca taaatgggca
     9301 gaccattgat ataacgatca tagaatatct tcttgcgcct ttcagtcaca tcaggcttat
     9361 gcggatgctg tatcgctgaa tagcctcgaa caaacaattt atgcagatac tcaaattctt
     9421 cttgtgcttc ttctttatcg atcagcattc tttctgcttc aaatatatga tcagctgtag
     9481 aagatggaac caaggaataa gatgctgtca cttttggttc tcgaggttga cctatcctac
     9541 atctagctga cagatatgct gaaagaaaaa caccaacatt atgttttgtg cgatccatat
     9601 ccacatcttt tgcctctggt gtctcatact tctttacatc gaaaagtacc atccattgat
     9661 tcctccattt atgatataat aattgtgtca gaattattaa ttaaggtcgg aggaatccgg
     9721 ctttttttat acccatgctt atgctaagct tttcaagtag cgaggttgca ctcgttaccc
     9781 atatacatgt tgagctatct ggcggaaaac agatggctca ctatttcaat attctgctaa
     9841 ggacagccag tggtcggctg tcttttttat attttaatga gaagccttac ttattttttt
     9901 atctttatta cgaaaaatga tattatctat tgataataaa gcgtatagga gatggttgag
     9961 tgactatgtg gagcatgctg ttattttgga ttcccgtttg tattggtatc gtcgcatttt
    10021 gttactttgt caaacactct agaacaaata agctcctcat gttatctttt ttacctatag
    10081 tattttttat tgtacaaatt gttaaatata cctatattga atcgcaagaa atattcattt
    10141 tttatgtggt aggtttattt atctctgtgg tctttttcat aatgatactt tcctattttt
    10201 ataaaaaata aatttttctc ttagaagtac attttttcgc tgtttatata gccttcatat
    10261 ccactaatcg aacaactgca atattcgctt tgctcttcgc tagctctttg tcacagtcca
    10321 tcgtattctc aatacgaata actgctgaat gattgtagac gtgttctaca tatccacgaa
    10381 atggatagat gaatccttct gcttcgcagc gaaccatgtc accgactttg aattttggtt
    10441 ccttacgtgt tttagggttc tttgtcggca tatctagcat taaaccgccg atgccgtggc
    10501 tactagcgta aaatccgtct tttagtttca ttatgcttcc tcctcaatcg tccaagcatt
    10561 tgtaaatttc tctaagctta ttagttcatc ttcatttaaa tatccgtaac catataagga
    10621 ttctaaaagg tcgtctttag aatatcctct aaagtcaagt ctgagaccgc ttaggatgta
    10681 tacaaaggtt ttatttccat aaaacttgat tgtgtatgct ttcatttcta ttcctcctgt
    10741 tctaatcccc attgagcgaa tgctgttaga acttcgaatt gctgtttttc tttcagtttg
    10801 ccccatgctt ttacataagg taacgagcta acgcctattt ctaatctttc atagacatct
    10861 tctgtgaaac tccacaacgt atattgaaga tcatttcttt taaattcatt ttttagaaaa
    10921 tcaaacacga tttgctggtt gtcgttaagt tgaattgatt tttctatttg tcttaaatct
    10981 tccgattcaa gtcgtagatg actgatttga gtcattttag cttgctcaac aattggaaac
    11041 cccaatccca aactatcttc taaattttgg agttcgcttt ctttttcaga tatgagttgt
    11101 tgcaaacttt tcatccttcc gccacctctt ccactggcac agcaaacggc cagtatcttt
    11161 catcaattgc tttgatttct ggctctgtta gctgataagc agatttttcc caccaacata
    11221 attcaaattt attaacaaaa caggaatcat ttctgttatt aaacttcttg acaagaaata
    11281 aattcccaat gataacttca tacaaaggct ctttctcgac ctcgtagcct acagcgtatg
    11341 cttgagcaaa taacagttgt cgttcatttt ctgttttgat tcgaggattc ccaaaaagcc
    11401 atgtataaac ttcgtcttct gaactgtcca tcatttcata taaagttgct cccccgcctc
    11461 tgtattcttc gatataatcc gccacaaact tcggcacaaa tggcttctgc ggttcgtcta
    11521 gttgtttgat tagttctaat gcgtgttcta aacccgcata ttttccatca tgaaaagtac
    11581 ttataccttt aaagtcttct atgctaccta caaggcttgc tagtttatca atcaattcct
    11641 gtttattcat cgctgtcctc catgtattcg tctaatatct ctttatattt ctctacaaat
    11701 ttgaaacgat cttgatgaag tttcttgctc caatttgttt gccgatccag ctcacgcatc
    11761 tgatcgaacc ctttttgaat ttcgttgtaa taaaattcaa tgtttgctgc tgctttccaa
    11821 tgcctgctac ttcgcactcc tgctcctgtt tcagccattt ctaacttaac taattcagct
    11881 cgttcttttg attttttgtc tttctgaatc ttcatcatga ttttcttgag gatgatgtca
    11941 ctgtattgtg taatgagatc cattatctct cctccacata cctaaactgt cgtccttttg
    12001 aatcaatcca taagctccta gctctatccc agataatgtt tttgctcaga ccagtaattt
    12061 cagataactg ttcagcagta cctgttacta gaattcggtc accatgccag attgcaatct
    12121 ttctcggcgt tctccgttta ggcttttcag tccacattga tttaccgagc ttttggactt
    12181 ctgcaactat ttctttgtct tcttgccaat tctcagaatg tgtcagttcg atgattcgtt
    12241 tcattgctgc tttcttatcc atccttaccc ctccaatcta cgaatttccc ttcttaaatt
    12301 ctctatgtgc aaatcgattg ccttcctcgc cgtttcattg accatcactg cctttgtccg
    12361 ttccagatcg tcaatttcac gttgaatgct tcgaattcgc atttgaatca cttcttctgt
    12421 tgtcatgatg attcctccac gtatctaaac gttctcttct tagcgtctgt gtagccacac
    12481 cttgctcttt tcctcacgat tttctcgtgc aagcctgtga gacttgctaa ctgttcagct
    12541 gtccctatca ctagaagttt gtcaccgtgc caaattgcga tttttcgtgg tcttggcttg
    12601 ttgctcttgt ctgcccacat cgctcttcca agcctcatca cttctgaagc agcttctttg
    12661 tcattttgcc aatcttctga ataagtcaat tcgataattc gctgcattgc cgctttctta
    12721 tccatcccga cattctcctt tcagtaattt gagtacttga tcaagtgcgc tctcacgtcc
    12781 gccgtggaac gtgttgagcc acttgtcttc gtacgataca ctttgtctta aagcttcttg
    12841 atgcattagt tcaatctgtg ctgtaaatgt ctttaggtcc atctgattac acctgctcaa
    12901 gttcattaag atgtttttgc aagcctttaa cacaatcaac aaatagtaat ttggtatatg
    12961 ctaaatttct taattgtgtt gtatcgatat aaagtgcgaa atagtatctg agcttactcc
    13021 aactcgaacg atcattctta attccttcga ttccagcttc ttcgagttga tcatatacgt
    13081 ctctcaggat ttctatttcc tcaccagttt tatactttgc tatttcatta attagttcta
    13141 gataatcgat tttcaatttc ccacctctta gaatggtgct tttgattgtc tattagctcg
    13201 ttctagcgct tttttcttga gataggcttc ttggtcgatt gcccactcag gaagcttctc
    13261 tcgtcttcct gtgcgcttgt atccactgct tgcgttctta gattcacttt tttcttttct
    13321 tgcccagctt cgaatagttg ccaaatagtt tttataagtc ttaccagatg attcacaata
    13381 ctcagatagc cgttctattc gctcttggta gtcattaggg aattctattt tgagtttctc
    13441 catctgctca tctgacaaaa gaacattttt atactctccg tatttatgac ggacgggctt
    13501 agccttcgat tttttcgaag gcgttacatt ctctatatct ttctctaact ctatatcttt
    13561 ctctaactct atctctaact ctggtgtagt tttgtccgga catttgtccg acatttgtcc
    13621 acttgttatt aaattctgtt ttgcttcctc aatttctttt ctgtattttc tcttcctatc
    13681 agcttcagtt gaagatttcc caataaaact ttgtatatca gacatataaa ttgctccgtt
    13741 atctaatacc tcaataagtt gtaaatcacg aaatatttgt actgcttttt ctacaactcc
    13801 cacagagtgt cttgtaatag tcgcaagcat tgtagaattg aatggaatcc tgtcattaaa
    13861 cattagtttg ccttcatgtt tcagacttct taaataaagt ttgagaagaa tattagaata
    13921 aatgtatcca tctggcatac tttctaaaag aaccatctcg tcgctatcga aaaagttctc
    13981 ttttagtttt aaatagtagt atcgtttgtt gtcagccaat attttttatc ctcctattct
    14041 aagtttctta attgtttcct ggtttaactt gatccctttg atttgatatt tatttttgaa
    14101 attgatcaca cctatcttgt gtttctccgt gtgatggatt ctgcagagtg ctgcaaatgt
    14161 gtactctgaa tgatcaactt ctttgcgctt tcgtcttcct agcgctttgt caaagtgatc
    14221 gatatcagct cctgttttgc cacagatgca acagactctt tttgtgatgc atttgtagaa
    14281 gtaatattct tgattcgctg gtaaaatctc atagccttct ttgaaaggaa tatgatgttc
    14341 aaagatgaaa tctaagatga tatttgctaa gacgttagca tcactcacag tcgtattcga
    14401 ttcatctttg aggcttattt tgcgccctgt gacaccttca aagcggaagt aaaagaattc
    14461 cttccagaag tccgttggca tgcctgtatc gatgaaaata tcgcctatga gtgcatagat
    14521 gaagtttcgt tgctgtactg tgaaacgtct aggatcaata aaacgaattt caataactcg
    14581 atcaccatcg tagccgtcat acatcgtctt tagtcgatca atgttcactt cctcattgat
    14641 ggttgcacct atgtcttttc ctttgaactt tttcagaacc gctgaatatg aatcgattaa
    14701 tggtttaaac actcatatca cttcttatct aattcttttc tcttagctgc tattgctcgc
    14761 tccatcaagg cacattgctc atagcttaac tgttcaatag tttcaacgtt atcagctaag
    14821 agccctaatt tatctgtctg ctcattaaca tattcgatta aggttttggt catatcttta
    14881 cccatctgct cattgaaagc ttctagaatc gtctctagca tgtttaattt cttcgtatcg
    14941 attctaggtg gtgttggaat atcttcccct tgaaatacat ataatcccag tccgtgtaga
    15001 gccaatgctt tcacaaagca tcgcttcaat gagttattga tttgcattgc atttggttta
    15061 acaactggtt ggtttcgata atctaaaaca ggaaataatt cggtttccgt gtgtccttta
    15121 accgttactg agacagatac ataagtccca gtttcatcca taagaaaagg tttatattcc
    15181 tcaacaagaa agtcttgatg agttccagaa acaactctgt agtgtttata ctcattaata
    15241 gttaccgttg cctgtggatc attctttttc ataatctccc atgcgtgagc ccaagataaa
    15301 taatcaaaat ttcctttttt cttgagtatt ttatttaact tgcgactaaa aagtttttca
    15361 aaagtcgttg tccctttgtt ttcactcatc aaattctgcc tccatttcag caatgtattt
    15421 cttacctgat ccgtaataag agatatcaat caagttatct ctgtcatact cttctagcgc
    15481 atcaatcaag ccatcttcga tgacatagat atattcaggt tttttggact tcctcgataa
    15541 atggataaga tagacatgat cccaaatact cacaaaattt cccaaatcgt cttgatcaca
    15601 tgctagttct tcatccgtca aaagatttcg tctgattttt cgattatttg tttccttgat
    15661 attcgatttg ccccaactag gatcagtcaa atattgatct agagtggaaa gttctttttc
    15721 catatgctaa aatctcctta tgatgtgttt tctttgtgac tctttgcttg ccggcggagt
    15781 cactttttta tttgttgcca tgctttttgc ttgtcaatat gttgttggct taggatgatt
    15841 ggtttattgt gtctccacca gcgattagca attaccgtcc cgattcttag cgcttcagct
    15901 ctattcattt tcatcaccaa aaagtctttg ttgtctgttc agttgatcga tttccatgcg
    15961 aatcgcagtt tctggtaacc acatttcaat aaatgaaaca gcatcatcga atctcttacg
    16021 aggtaactcg ccatatcttg ggattgaaaa ggtacgttta aattcagacc aaaattttga
    16081 gaatactttt ttgctgattt cttcataagc tcggctttct tttcccccta gaacttccat
    16141 aactttcgta tttccttttt gcttaatttc aaactcttgt tgtccgctaa ttcgcatagt
    16201 atttttaagc atggaaacat cttttttaac atctttcatt tcttctaatt gatagatcat
    16261 catgtcttca attgtgtcaa aagctgtatt tttgcgaatt gccttttcca tttcgttgaa
    16321 cgcttcaatg tatttcaatt tgaactctaa aactttttga gtattgttga atcctaatac
    16381 agcaagagta aagccatcac gattcatata gacaattcga taagcctgtt tgttttgcgg
    16441 atggatgtac acatcttccc aaaataggtc tgcatacttt tgtgcaagcc catcttttag
    16501 atcgtcaatc gcctttaaaa ctgctctatg ttcctttcgg aatgtttctg cgacttgtaa
    16561 gctcgtagtc acagcttctt tatttttcaa aattactaat tcttgcattt ttttctctcc
    16621 cttttggtat aatttaggta aaaaggtggt gtatataatt ggatatttca gatactcctt
    16681 attatcaaaa attgatagct tcttcaaaat taatcggtga aaccattgct ccttctctag
    16741 atgtcgttaa cgctatgcag cctgcgatag aacaagctaa aaaattaatt gcaattcaag
    16801 atacttttat agcttcgcaa gcaatactca atgattctct cattcaaatt gtcgagaaac
    16861 gtaacaaatc tattgctaat atgattcctc cttctttgtt cgcaatacag aaacaattag
    16921 aatctccaag ttttaatatt gctaaaaaaa tggcagctac tatgccaaaa ccttattttt
    16981 ctgatatctc taaaatcaca gaaagatttg ccgttgacat gtctaagtac gacttcagaa
    17041 taggggtagc tacccaagaa ttagaaaaag cattttcttc cgaaccggaa gttagtacaa
    17101 aaaatctact ttttaaagac acttctaatc catctgatga gtttgaggct aagttttttg
    17161 atatccttaa tgagcaaagg aaagtccttg atgattctat aaagcttttt gaagaaataa
    17221 atacagccaa gaacaaagat ataactaatg aaccatctga aaaacaacaa gcttcacctg
    17281 ctttctacaa agataaaatg tggtatttag agcaaactgg ggcagctttg ataggactag
    17341 tagtaaccga aattgtaaat ataactattg gagtagatcc taacaataca atttcattag
    17401 ctttattttt gcgttatttg ttacaatttt taattaatta ggttaagtca gtccctcccg
    17461 actggctttt tcgctctata ttcagcttca tcaagcccca taaagatcca gaccatatat
    17521 acaattgtgc cgattaatgc ttgtctgctt ccccaaagac ctaaagcgta gataattagt
    17581 ggtgcgctga atactagtgc tctattaaat ttacccatcc gcttacctcc ttaagatttc
    17641 cgaaaaattt gtttctaaaa attcaagtgt tttacttctt aaaaatagat atgtgtctct
    17701 tccttcaact ggataataga caaatccatt tttgtttttt tcgatatcga taatatttct
    17761 atatcttggg ttctttaaaa ctctagaagt aaaccaatca tattttctgt taatccgttc
    17821 tagcacttct ggcaacgtca tccatctacc agtatcatca gcttttttta actcctcata
    17881 atccacttgg gagataatta catagccttc tggaactggg attttcgctt ctagatattg
    17941 cattagctgt tcctcccttt acaattcgta catagtaata atcgaatcta tgattctatt
    18001 tgcttctgca gaagtctttt taccgtttaa aattaaagat aagtagcttt tgctaatgcc
    18061 gaatctttca gcgagcatgg tgtaagttaa gaactttgaa ctttcgacat attctttgat
    18121 tttttctcta tctcgttgag tgatttctgc aatgtcagtc atactaaaac tcctttctaa
    18181 ccaatttcct ctaaatccat ttgagggtaa tatccttctt tttttagtaa ttcgtagata
    18241 aatagacgcc ctttctgtgt ccatttggta ttcattacaa ttttagtgcc accatcggct
    18301 ttcgggatct cagttgtatg agattttgtg tatccttggt tcatgtgttt tttgcacaat
    18361 aaccattggt taccgacttt tttctgaata cctagtttat gaagtaattt attcatctgt
    18421 tgtggagaca tcccataatc tgctgcaatc tgactaattg ttactgaatc tgtagaagat
    18481 aatatgctat ctaaatagga gattttaggt tcgtactctg caatcttttg ttctgcgatt
    18541 aatcttccag ttctttcttc ttttagttga gttgctaatt gaatgattgt atctggatta
    18601 agcaaagctt cttctacttt ttctggagtt agataacctc catgttttct aattgctggc
    18661 aacacttcac ttgttaccca acgtttaaat tttttggcag agggaagttt tgattttaag
    18721 attaaactgt aaaggcctga ctcgttaatc aaaacaactg ctgttccatt gacggtgaac
    18781 gaatcgttct gcgtcttatc ctccaagtcc acatgatctc gaatagcttt ttgcggattc
    18841 gaatatccta gaacgtctgc tacatctttt cctacaaaat atggttcatc atttactaaa
    18901 atagttcgaa cttcgttttg ctcgaaatta aaaatttgtg gtgtattcat atttctcatt
    18961 cctttctttg gtataatttc cttatcagtg tgacaggctg aaataattgt taaggaggtg
    19021 aaaatttatg aaaccagaat ttgtaattac tatgcttgat ggagaaaaaa tcaaaatatt
    19081 tgccgatact ttggtcgttg gatacgatga tgcgtcaaat atcgaagaaa aagatggaat
    19141 gttctattct tctcaaattt acgtgaatac cttgcaaggt gatattgggg tatctactct
    19201 tgcaacatca agaccgcttg taggtcttat cggctttttg tcaaaagcaa cattcttttc
    19261 tctcggcgat gatatagatt cggatgaaac tgtcatctat aaaacatcag ctgttaaaag
    19321 tatcggtttc aaatagtaat tcttgctcgt tgatagcgga agccaacttt tcttccaaca
    19381 catcggcgag ctttcttttt gttggattca tttcaaagtt gatagtcttg agaagcattt
    19441 ttgcatcctt aactttttgt ccttgcaatt ccttgatgat tttatcggct tggctatcaa
    19501 ttccatgtgt tagatcatct atcatttttt gtaccttgat tgtttggctc atttccttgt
    19561 tcattgttta gcctccttta ataaatgatt ttttgtattt tttaatttcc ataaattcct
    19621 caaattgctc tttggtatta ttaccacgac catacatatt gtgaaattct ttatggcaac
    19681 tgacacaaag tgtaacgccg tttgaaatcg ctgcggattt agttgttgca tggctaaatc
    19741 acgaatcaca agtatctact aaaggcgaat caatttcgcc aaaagaaatt gttcaagctt
    19801 atttagatat tcattatgct gttatctatg gtcagcttcc agaagaccgt aaaaacgatg
    19861 actaaacgtg agctaaaatc tctgctatgg ctgcaaccat ggcagagtct tctttatcta
    19921 ccgcgtgttc catcgccact tttgcttgtt tcagtatgtc taatttcaaa tcttcgattt
    19981 ttgccgagac tgttttttcc atattcttct ctcctttctt ttatattcgt aaacaaattt
    20041 aacaactttt taaaaaactg cgttgactaa attaacatac agtgttaaaa tcagaacata
    20101 gttaaataag acatataaac attgatttta aaagctttct tggcggttgg catttattaa
    20161 tcaatagtgt tttttgttgt ctttttagtt gttaaacttg tttacaagaa tgatattaac
    20221 accttttgtt aaaatagtca acgaatttta acaaataaag ttaaatattt tttcttgaac
    20281 gaaaggaata ttgatatgat aacgtttgag agaataaaag agttagcgaa aaaacaggga
    20341 aaatcattaa ataaagttga agaagaccta ggctatggta aaaacgtctt atatagactt
    20401 aagaactcaa acccctctac agaacgtttg caagaaattg ctgactattt cgatgtctct
    20461 gtagactatc ttctggggcg cgaagaaaaa gaaaccccta aacatgtgga tttatcagaa
    20521 aacgatactg ttttttcttt tgatggaaaa gaaatatcta aggagacaat gcgtaaagcg
    20581 attgcaattg ctaaagcttt agaggaaaat gaatagttgg agtgatgggt tgtatgtatt
    20641 tgaaattgaa agaaatgttg agcgaataca atttgaaagt agtttacatg gaaatgaaag
    20701 agcctggctt ctattatccg aagcctagaa tcattttttt gaatgaaaat ctatatggcg
    20761 aaactgcaga agcctttcat ttatctcatg aacttgcaca tttcagcgcc tctcattttg
    20821 aattttcagt tttgtacgat acttctacaa cttttcactc aaaatttgaa acagaagcag
    20881 ataaaatagc tattctaatt ttattaaata tctatattga gaacgaactg actgatgaat
    20941 ctcagtttaa cctcgaaaaa tttatggaat attattctat tcagaataag ctcagataca
    21001 cttgttatgc tgtctgccaa tgctatttta agaaaaaata ttcttacgca aggcaatatg
    21061 tatgaataca tcaagaatta ttaaaaaaac cgatttcagc aaaacattaa aaaagcccgt
    21121 gctggcacac ggactcatac ctcatttctg agatcgcaaa tatattataa caagaagtga
    21181 ggaatattta aatggcaaaa aaagttatgg gtcaagacgg aaaaatgtat aaagttagta
    21241 aaccttttta caaaaaagta tggttttggg tattagcagt aattttaatt ataattattg
    21301 gttctgcttt aaatggtgga tcagatagta ataaagcaag tgataatggt ggcgaaaaag
    21361 taactaaatc ttcaacctct gcttcatctt ctaaagaaga aaaaagcgat accttctata
    21421 agattggtga cactgtaaaa gttggtgatg ctgaatatac actaaatagt gtagaactaa
    21481 ctgatgaaag aaatcaattt gaagaaaacc aacctgcaca agtagtaaaa attacttata
    21541 ccgtaaaaaa tgatggcgat tcagatatcc ctgtaggtac agatgtggaa gtatatggac
    21601 cagatgacaa aaaatcagag acatatgcca acgaaaatac aatgggatct gttgctccag
    21661 gaaaacaaat ggatgtaact gctcatttca cgttaaacca aaagggagaa atagaaatcc
    21721 acttctctcc tttagtatca tttgaaaaag cagctatttt taaagcaact gtataataaa
    21781 taaaaaacac gcccaccgtc caaagcaagc gtgttctaag aaaaacaaac ctatacagta
    21841 ggcttcttta tagtgcctat tgtatcagag aaagagagta gattcaatta tggcaagatt
    21901 agtcaaacgt ggaaatagtt ggcaatacga aatttcatac aaaaaagatg acggaaaata
    21961 cacgaagata agaaaatcag gatttaagac aaaaggcgaa gcaaaagatg ccgccaacga
    22021 attagaatat aacttgaaca aaggccttaa aggggatcgc aaaaatctat tattatcaga
    22081 ttactttgag gattggatgc aactttataa agaaggaaca gtatctccta tcacttatag
    22141 aaaatacgaa gatacgttaa tgaacataaa gaaatatatg ccagcggtat tgatttctga
    22201 tttagataga gttggatatc aacgcttttt aaataagtat gcgaaagacc atgtaaaatc
    22261 caccgttatt aagtttaata accatattag agcatcgttg aaagatgccg tagaagaagg
    22321 attaattccg tttgatccaa ctagaaaagc agtaatcaaa ggaaaagatt cattgaagcc
    22381 aaaagaagat aaatatttag attatgatca atttaaatct ttaatgaaac tcgtagaaga
    22441 aaacctttct gcgcagtact cttctcctat gctcgtgtta gttgctggtg ctactggaat
    22501 gcgatttgct gaacttctag gattaacatg ggaagatatc gatttcgaag atcaaatcat
    22561 cactattaat aaaacatgga attataaatt aaatgaatgg ggaaaaacaa aaaacgaaac
    22621 ttcaaatagg aaaatttcca ttgataaaca tacgattgat ctcttaaaaa agtttaaaat
    22681 caatcaaaaa gaattattcg agaattttga agttaaaaac cctcataatt ttgttttttt
    22741 caacttaaaa aatggattag tttcatcaaa cgccgtcagc aaatatttgc gcaaaaaatt
    22801 aaaagaatta gggattgaaa agcaatttac tttgcatgga ctaaggcata cacatgcatc
    22861 tattttactt tatcaaggag taaatatact tagcgtatcg aaacgtttag gacatagcag
    22921 tttagaaact acaatgtcta cttatcttca tattgttcga gagcttgaag atcaggataa
    22981 agaaaaaatc aatactgtgt tcgatagttt atataaaaat gataactagt ttggcacatt
    23041 tttggcacaa atacaaaaca aacccctgta cc
//
LOCUS       DACDIE010000042        22985 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.042, whole genome shotgun sequence.
ACCESSION   DACDIE010000042 DACDIE010000000
VERSION     DACDIE010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22985
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.042"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(109..564)
                     /locus_tag="IV250_001806"
     CDS             complement(109..564)
                     /locus_tag="IV250_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769089.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(718..1926)
                     /locus_tag="IV250_001807"
     CDS             complement(718..1926)
                     /locus_tag="IV250_001807"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAP8769090.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2038..2370)
                     /locus_tag="IV250_001808"
     CDS             complement(2038..2370)
                     /locus_tag="IV250_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP8769091.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2518..3393
                     /locus_tag="IV250_001809"
     CDS             2518..3393
                     /locus_tag="IV250_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8769092.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3475..4056)
                     /locus_tag="IV250_001810"
     CDS             complement(3475..4056)
                     /locus_tag="IV250_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAP8769093.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4062..5321)
                     /locus_tag="IV250_001811"
     CDS             complement(4062..5321)
                     /locus_tag="IV250_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP8769094.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5323..6369)
                     /locus_tag="IV250_001812"
     CDS             complement(5323..6369)
                     /locus_tag="IV250_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769095.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(6604..6879)
                     /locus_tag="IV250_001813"
     CDS             complement(6604..6879)
                     /locus_tag="IV250_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAP8769096.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(7122..8432)
                     /locus_tag="IV250_001814"
     CDS             complement(7122..8432)
                     /locus_tag="IV250_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAP8769097.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(8614..9843)
                     /gene="rpsA"
                     /locus_tag="IV250_001815"
     CDS             complement(8614..9843)
                     /gene="rpsA"
                     /locus_tag="IV250_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAP8769098.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(9978..10658)
                     /locus_tag="IV250_001816"
     CDS             complement(9978..10658)
                     /locus_tag="IV250_001816"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAP8769099.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(10707..11342)
                     /locus_tag="IV250_001817"
     CDS             complement(10707..11342)
                     /locus_tag="IV250_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8769100.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(11408..12811)
                     /locus_tag="IV250_001818"
     CDS             complement(11408..12811)
                     /locus_tag="IV250_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAP8769101.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(12798..13829)
                     /locus_tag="IV250_001819"
     CDS             complement(12798..13829)
                     /locus_tag="IV250_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769102.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            13860..14081
                     /locus_tag="IV250_001820"
     CDS             13860..14081
                     /locus_tag="IV250_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAP8769103.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(14246..14983)
                     /locus_tag="IV250_001821"
     CDS             complement(14246..14983)
                     /locus_tag="IV250_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAP8769104.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(15136..16476)
                     /locus_tag="IV250_001822"
     CDS             complement(15136..16476)
                     /locus_tag="IV250_001822"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAP8769105.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(16713..18236)
                     /locus_tag="IV250_001823"
     CDS             complement(16713..18236)
                     /locus_tag="IV250_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAP8769106.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(18614..19225)
                     /locus_tag="IV250_001824"
     CDS             complement(18614..19225)
                     /locus_tag="IV250_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP8769107.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(19348..19473)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(19555..20271)
                     /locus_tag="IV250_001825"
     CDS             complement(19555..20271)
                     /locus_tag="IV250_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP8769108.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(20277..20846)
                     /gene="scpB"
                     /locus_tag="IV250_001826"
     CDS             complement(20277..20846)
                     /gene="scpB"
                     /locus_tag="IV250_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAP8769109.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(20830..21627)
                     /locus_tag="IV250_001827"
     CDS             complement(20830..21627)
                     /locus_tag="IV250_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAP8769110.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(21597..21968)
                     /locus_tag="IV250_001828"
     CDS             complement(21597..21968)
                     /locus_tag="IV250_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP8769111.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(21987..22874)
                     /gene="xerD"
                     /locus_tag="IV250_001829"
     CDS             complement(21987..22874)
                     /gene="xerD"
                     /locus_tag="IV250_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAP8769112.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         6445 a         4911 c         3900 g         7729 t
ORIGIN      
        1 aaatcttgtc acagtccctt attctgaata aacggtgtgg aagagacagc ctatcagtag
       61 taataagaca catctctttc acagcctcat ttttgcgtaa aaatcgattt attcgaaaaa
      121 ttccccgtat acatcttcta cacgttgatc tggattgatc acgatataca agttacctgt
      181 tttttcgctg atttttgttg attgatattg attgtctaat ccttctgttc ctttatctag
      241 atatgggaag tacacaagat caggtgtgcc atttcggtaa aggatttcca ttcgctcgat
      301 atcttcgtat ttcaatgcgc gattaaacat gcccatttcc agtccgccta ggttgatatc
      361 atgtgtcgca actgcgtccc cttcatggaa gatttcgatt ttaaatccag cacacggatg
      421 gatttcaaca aattcactgc cattgattcg tccaaaactt gttgtgattt gtttgatcca
      481 aagatcgccg atatgacggc ggttgatcgt ccaggtttct ccatttctaa aataaaattt
      541 tagcgctgtc atttctttac tcattaaaaa gactccttta tgatgtaatt tattcttgta
      601 ctacctcagt ttagcacaaa tttatgtaag tggtcacatc ttatgttttt tcttctaatg
      661 acgtatctgc ccactttaag tagccaacaa agctgagcag atacagtcta gtgtttttta
      721 aatcaatttt tctttcgata aaaaatccaa aacctgtttt ttatcatttt ttattctttg
      781 ttctacgacg gctttttcag ctaattgcaa agcttcacta acccacttac caggtttttt
      841 tccagtttct tctagcaaga ttttaccatc tactgctaat tcgcttaatg acttgatagg
      901 taaacgatca taggattctc ctacggcatc catctgaggt gtttcgtcaa aatagatcat
      961 caattcttct acttgcagtg catgtttttt gcctaagaag tacaattcga atggttccaa
     1021 agctccagtt tctcgttgtc gtagtccttg gacaagtgct tgaacgtctt taatcatttg
     1081 gttagaacac ttccacgatt tcaaaaagga acgaatctca ttttctttta gatccagttg
     1141 gctgatcaat aatgcccatg cttggctctc ttctttcaat gggacggatg gtaattcagc
     1201 aaaacgtaaa agtgcttcgc cttcttcttt caaaccggga cagcaaatgt agcactctgt
     1261 ttcaataaat gcatggattc ccgcggcccg ttcatttccc aagagcatct tgatgaattc
     1321 cacatttata cgttctacag atattttacc taatagctcg tggttttctt tgatggcgta
     1381 gaaggtttct tcctctaatt tgaaccctag ttgactgaca aaacgcaacc cgcgcatcat
     1441 tcgtaaggca tcttcatgga accgctcatg aggattgcct acagctcgca agatctgttc
     1501 ttctagatct tgcagaccat cgaacaaatc gatgatttct ccatcttctt tcaaagcaaa
     1561 cgcattgatc gtaaaatctc ttcttttcaa atcttctgcc aacgaacgaa cgaattcgac
     1621 atgatctggt ctacggtagt cctgataagt tgattccgtc cgaaatgtgg tgatctcata
     1681 ttgttcatca ttttctaata ccaacacagt tccatgttcg atcccaatat ctatcgtttt
     1741 tggaaaaagc tgtttgattt cttctggaaa tgcgcttgtt gcgatatcca catcatggat
     1801 tggctggttc agcaagatat cacgaacact tccacctaca aaataagctt catatcctgc
     1861 attctctaat tttttcagaa ctggaattgc cttttggtat tccagaggta attgttttaa
     1921 tttcattttt ccaacctttc ctgcgtgttt cttgtcctga gataaagact cagttgcatg
     1981 gtgactgttt gacaagtccg tttcagagtc ttcaaggcca ggaccacttc atcttgttta
     2041 ctcgtcctta cgctcaaagc gattatggtc tctttgattg aatttttcca tgtttttttc
     2101 aaaaacttca gttaagtcga tattcaatga attagccatg atcatcgtaa cgaataaaac
     2161 atcaccaagt tcttctgcta ccgagttaac tggttcagaa gcctttttgc ttttttctcc
     2221 ataagtgtgg ttgacttctc ttgccaattc acctacttct tcagttaatc gagccatctg
     2281 agctaatgga gagaaatacc ccaccttgaa ttgctggata tattcatcca cttcctgctg
     2341 catactgtat aaagaacgct ttttatccat ctttttccct ccaaaactat cttaccaaaa
     2401 ctttaacgca aatcctagat aagagcaatt gagaaaatcg aatttccatg ctacatttag
     2461 gaaggaattt ctaaatggaa attctgtgaa aatacataat tgaaagctgg aattcatatg
     2521 gtcaaacgaa aaacagtaaa agaagttctt tttatcatta taggaacaag tatttatgca
     2581 tttggtcttg tttacttgaa catcgctaat catttagcag aagggggagt ctcagggatc
     2641 accttgatct taagagcttt atttggtatt gatccagctt atacgacttt attaatcaac
     2701 atcccattga ttttactagg aggaaagatc ctcggtaaac gttccttggc ctatactgtt
     2761 ctaggtacac tttctttgtc attcttttta tggatctggc aaagggttcc actagaaatc
     2821 aatttacagc atgatctact gatcgtttct ctacttgcgg gtttagttgc aggtgtagga
     2881 agcgggatcg tctaccgcat gggtggaaca acaggcggca gtgatattat cgctcggatt
     2941 ttagaacaaa attacggcat cactatgggg cgttccctat tggcatttga tatcatcgtc
     3001 ttacttgctt cattaaccta tatcgacttg aaacgtatga tgtatacgtt gattgtttcg
     3061 tatgttttca gtcgtgtgat tgattcgatc ttagatggcg gatattctgc aaaaggtcta
     3121 cttgtcgtct ccaacaaaag cgaagagatt gcaccactac tgatgaccgg tctgcaaaga
     3181 ggtgtgactt tcctttctgg agaaggcgca ttctctggta attcaaaaaa aatcatctat
     3241 atggtcgtta gctctagtga attaactgaa gctaagagga tcgtccacga gattgacgaa
     3301 aaagctttcc tttcaatcat caatgtgcat gaagtggaag ggcaaggctt tacttatttg
     3361 aagccacaga caagattact gaaaaaacta taagaaaaaa gacaaagata ggctatgagg
     3421 aaagattcca catccagctg acctttgtct tttttagtac tatactattt ttaactaatt
     3481 gttatgttca cttacccggt tacttttctt ttgtgtctgc aaaaggatct ttcggttaac
     3541 ttcatcagat aatttcaaaa aagcatccct atctccatct tctagtgctt gatcgatttg
     3601 agcataaagc atttttagtt ttgcctcttc ttctttttct tgaaaatatc gttcaattaa
     3661 ttcagtatct tcttcactga ctgaagaatc ccaagaagaa aatggattgt cttctaagat
     3721 agatagatat tctcttgtct gccaagcatt ttcgaatata cattccacat ataaaggctt
     3781 tttccaattc aaacggattt catgaaaaat ctggtctgtg tccgtaaatt cttttccctg
     3841 caaaaacaat ttcatgggtt ccccttcaac agatagatca ctgatctgca gtcctcgatc
     3901 cgcaaaattc gccccttcaa caaagtggac attgcttaaa atcgcttcat gattggcaag
     3961 ataattcaag atccaaaaaa cttctcttcg gctaaaagat acatgattga ccatccacgt
     4021 taaaaaattc ttcttttccc gtacatcaat tagcattttt ctcacctctc tgttaggtca
     4081 tctaataatg acagtgcctc catatctcct ggttcaagtg ttaaataata agtcaataat
     4141 tcttgcgctc ttttcagctg cccttcttct cttaagaaaa aggcatactc tttcaagaaa
     4201 tctggttcat gtgatagttc atgataagcc tgttcatagt gtacagctgc aacggtaaag
     4261 tcttctaatt cattataagc atgggctagg ttccattctg cataaggatt agcagtttct
     4321 tccatctgat tgatcacttg tatcacatct tcatatcttt catcatccaa gtaaagatta
     4381 cttaaagtaa gtaatgtttc atcttgtttg tctcctactt ctaaagcttt cagtaaaagt
     4441 tcttctgctc gctgtctatc atgcagacgg aaagcatttt ccgaggccag gtgataaagt
     4501 tcgacttggt aaggattttc tttgattcct tcttctaaca ctgtttgtgc ttcttcaatc
     4561 atttcttctt cttgaagagc ttgtcctaga tatagatata acgattgata atgcggatta
     4621 acttctcgaa gttcttccag atagttgatt gcctgctgat tttctttcaa ttgaagattc
     4681 gtaaaagcta gctggaacag tcgatcatct gtcctttctt ctttcagtgc ttcttctaaa
     4741 ggtacgatag ctgcttcgaa ttctccttgc atacttaagg attgtcctaa acgttcctgc
     4801 atcgagattc ctgaaatttc atttatcccg cttgccaaca atgtttcata aaccactgct
     4861 gcctctttga aacgatcaac tgaaaaatac aattcagcta gcgcaaactg gatcaatggt
     4921 tcatcaggaa gcaacccagc tgcttctttc aattttgctt cactcacttc agggattccg
     4981 ataacttggt acaaatcagc tatcgccaat aagctttgcg gatagtaatc actttgctta
     5041 tccacttttt ccaggtattc aaaagctaag tcgatctcat tatcttcgat tgcaatttct
     5101 gctaacggga tgttcagtcc gtcattttct gggtattggt tgatcagttg ttcaaaaatc
     5161 tgttttgctt cgatcaaaaa gccaatagcc agcaattctt cgcctagttc agctaatata
     5221 tctgctgggt cttcttttaa tgctttttct aaaaagattt gtgcttgtga caagtcttca
     5281 tctgataaag cttgcaacat tttttcgcta tttgtattca tttcaattcc ctcttatctc
     5341 actgacgatt tgttgaaata ccgtcattgt tctcaataat tctttttcgt ttggatgttg
     5401 gtatccgtct attataccta ttttttctaa aacaagtatt tttttgcttt tttctgccaa
     5461 aaaaagaact ttttccaaat actccatctt tgaaatctgt tccagccagt ggattggata
     5521 gccaagcaag tccagccaca caaaaaagtt ttttagttgg aaatcaaaag gatatgcttg
     5581 gagattccac acgaaatgaa acatcattcc taaaaatttt ttcatactag cagaaagtag
     5641 atggccattt tcggtcagat aaaaagcttg ttcaaacagt ttaccataat ttgacaggtt
     5701 ttctgcctct aattgataaa aatagatcat ttcttcgatc atcccggcaa acggccgagt
     5761 cgttaattgc tttttatttg gatagttttt atacagattt tgcaaaaaac gatgatcaca
     5821 aatcattccg caacagataa aagtcaaaaa gtcaatctgc tttccgtcca cctgccgctc
     5881 gctgattgtt tgatcataaa cgatgcgttc tggcagattg atggtttgca gcactacacg
     5941 atttggtagc tgcaaaatcg tccgctgcgg tgttaatgct cggcttagag aacggatcga
     6001 tacagggatc atccatagtg atgaatcaag ggttgtatgt ttttgaaaaa aacctgccag
     6061 atccatgatc ccttcatttc caaatccgat gataaacatg gattgatttt cgggatagcg
     6121 tttgacgaaa tcaagcaggt tctttaattc tgttaaatga ttgcattgag tgttggcaca
     6181 aatataccag tcattgtttg cttgatttgc aaataacggg ttgatcttat ctgcaaacaa
     6241 atcataatag cgctgattcg ttaaaaataa gattggctga tcaatggaaa gttctcttag
     6301 ctgactgact agcgtttctc cgtaaaacac tttcgtcaac gcatcttcta cttgataatc
     6361 aagttccata cgcatccctc ctcttcttat ttttatttta ccattagtaa gcaaattcta
     6421 gaaaagataa gagcaaagct cacctcataa cttggaaata aataaaaatt aaataaaata
     6481 catgtgtttt ctatgtatga catacggatt cggaatctct gtctcttatt tattcctttt
     6541 ctgctaggaa ataaaaagaa aagcacttgt agccctagag ccacaagcgc tttttcaact
     6601 gtcttattta acagcgtctt ttaatgcttt acctggtttg aatgcaggta ctttgctagc
     6661 tggaatttcg atttcttcac cagtttgtgg gttacgacct ttgcgagctg cgcgttcgcg
     6721 aacttcaaag ttaccaaaac cgattaattg aactttttcg ccgttagcta aagcatcttg
     6781 aatagttgaa aatacagcat ctactgctgc agttgcatct tttttagtta aatcagtagc
     6841 tgaagcaact ttttcgatta attctgcttt gtttgccatg gatagattca cctcctccac
     6901 aagagatatg atagaaatat catttagtta aaactttctg agtggtccag aagtttaaaa
     6961 agaaatattg attaacttca atattctgtt atcacgatat catatgaacc gcttaatagc
     7021 aaggttttta ccaattttca catgcttttt ttagtcatag aaacgtaaca aacgaaactg
     7081 tctttttctt ttgaaaaaaa aacgtcgata tggtaaaatg cctattttct cctacgaggg
     7141 ataattcgga tggacgtccc ttcaaaagta aatgcttttc tgatttgatt ttctaagaaa
     7201 cgttcataag aaaaatgcat caattcctct tcattgacaa aaacgacaaa tgtaggtggt
     7261 ttgacagcga cttgtgtagc ataaaaaatc ttcaggcgtt ttcctttatc tgttggtgtt
     7321 ggattgattg ctacagcatc cataatgata tcattcaaca cagccgaaga gatacgaaga
     7381 ttctggttca tacttacttg ttcgatcatt tcaggcagac gttccaaccg ttgtttagtc
     7441 actgcagaaa caaaaacgat tggagcgtaa tcaagatacc ggaattcatc acgaatctct
     7501 tgttcgaaat cacgcattgt attggtttct ttctttacaa gatcccattt attcacgacg
     7561 ataataattc cacgaccagc ttcatgggcg taacccgcta ctcgcttgtc ttgttcccga
     7621 atcccttctt cagcattcag taccatcaaa acgatatcag aacggtcaat ggcacgcatt
     7681 gcacgcatca cgctgtattt ttcagtattt tcgtatactt ttccgcgttt acgcatacca
     7741 gctgtatcta tcattaagaa tttctgacca ctttcagaaa cgaagtgggt gtcgattgcg
     7801 tctcgggtcg ttccttcaat atccgaaaca attacacgtt cttctcctaa aatcgcattg
     7861 atcaaagagg atttaccaac atttgggcga ccaatcaaac taaacttaat cgtgtcgtcg
     7921 tcctcttctt cagtctcttc agagaaatac ttcactgctt catctaaaat atcaccaatc
     7981 cctagtccat ggcttcctga aacaggataa ggatcgccta gccctaagga ataaaattca
     8041 tagatatcgt ttctcatttc tggattatct actttattga ctgctaaaat cactggttta
     8101 ttacttctgt ataaaatttt cgcaaccatc tcatctgcgt ctgtcacgcc ttcgcgtccg
     8161 ctgactacac aaatgattac atctgcttct tcaatcgcga tttctgcttg atgcttgatc
     8221 tgatccataa aaggttcatc gccaagatca attccgcctg tgtcgatcac gctgaattct
     8281 cgacctaacc attcaccttt tgcataaata cgatcacgag ttactccagg agtatcttcg
     8341 acaatcgaga tacgctcacc agcgatgcgg ttaaaaatcg tagacttacc gacgttcggg
     8401 cggccaacga ttgcaattgt tggatttgcc atattatttc ctcctcacaa gtttccaatc
     8461 tcacttagtt taacgaatgc attgccaact ttcaagcata gattgtcaat taaacgaagt
     8521 atttttattc cttattgaaa tactaatcgc ttttcaaaag aaaaactgag acaaaagcag
     8581 ctgctttcat ctcagtcaca gatacaaaat caattatttt tcagaatcat cggttgcttt
     8641 ttcttctact tcttctttca acgcatcacc taaaatgtct cccatagtaa atccagtatt
     8701 ttcttctggt aattcataag actcaacatt ttttggttct tcttctgaag ctggtttttc
     8761 ttctaaagct ttgatactca atgcaatacg atgttcttct ggatggatct ctaatacttt
     8821 cactttcact tcgtcacctt catgaagcac ttcatgtggt gtcgcaatat gtttatgtga
     8881 aatttgagag atatggacta gcccttctac tccagggaac acttcaacaa aggcaccgaa
     8941 gcttgtcaaa cgtttaactg ttccgtctaa tactgtgcca actggagctt ttgtatcgat
     9001 ttctgaccaa ggtccaggct gtgtgtcttt gattgacaag gaaacacgtt cttgttcagg
     9061 attgatagac aacactttga catttacatc atcattgaca gccaatacat cacttggttt
     9121 tgctacatga ctatgtgaaa tctctgagac atggactaga ccgtcaattc cgcctaggtc
     9181 gacaaatgcg ccaaaatcag taagacgtgc aacacgtcca ctgagaacat ctccctcatg
     9241 gatagttgaa agaatttctt ttttcttttc ttctctttcg ttttccacta ccgctttatg
     9301 cgataagatc aaacgatttt ctgatggttc gatttcgatg attttaaagc tcaatgtttt
     9361 gcctttatag tctgaaaagt ctgcaacaaa atgatcttcc accattgatg cagggacaaa
     9421 accacgtaca ccgacgtcaa cgactaaacc gcctttgaca acatttgtaa caggtgcttc
     9481 gatgaccttg ccctcttgga aatctctttc gatatcttcc caaacttttt tagcatctaa
     9541 acggcgtttt gaaagtaggt agctgccgtt ttctttatct ttgccgatcg tactgataac
     9601 gactaaatct aatacatcac cgacttttac tacttcgctg atatcttcta ccggcaaagt
     9661 tgataattct tttgccggaa cgactccttc aactccagcc ccttcgattc cgacaatcac
     9721 ttgtttgtct tcaatgacta aaacttcgcc cttaacaaca tcgccaactt ttacttcttg
     9781 tacgcttttc attgcgtctt ccattgactg gttgttctct tgattttgat caaattctgt
     9841 cataacactt gtcctcctaa ccaacattct gccgtaagta cggtactgtt tttagtttaa
     9901 ttgattattc aaaaaaaata aagcgatttc gttcgttttc cttgaaaaaa caattgtttt
     9961 tcattttttt tcttaattta aaaaagaaga taaccttttt tcaaaacaac agcttttatt
    10021 gctgcaacga cttctggaat cgatttgcca gtcgtatcga tacgaacagc atcctctgct
    10081 tgcttcagcg gagagacgtc ccttgttgaa tcataatggt ctctttcctg tatctctttc
    10141 gtcagtgttt caagatcagt cggaattcct ttttcttggt tctctttgaa acgtctttct
    10201 gcacgctcac ttacgcttgc tactaaaaag attttcactt ctgcattcgg taatacagct
    10261 gtcccgatgt ctcttccgtc catgacaacg ccaccgcgtt cccctatttt tcgttggaca
    10321 gcaactaatt cttctctgac ttttccatgt gcagagactt cagaaacagc ttttgtgaca
    10381 tctggttgac ggatttctaa agtcacatct tttgatgcta tgaatacatg ctgtccatct
    10441 tcttgttgcg aaaaagagat aggatacttt ttgatcagcg aaactagccc ttcttcatct
    10501 gaaaaagata cattgttcgt aatagctaaa taagttactg cgcgatacat ggcaccggta
    10561 tctgtataaa tataattcaa ttcttttgct aatatcttgg caacggtact tttcccagat
    10621 gaagcaggcc cgtcgattgc aatactgatt tgtgtcatga tataactcct ttttctcaga
    10681 aaaaaagccg caaagcgcgg ctattattat ttaattctta attcttgacc agggtaaagc
    10741 ataaagttgt ttggatctaa gccattcaat tcaaacaatt gatcagtggt gatccctgca
    10801 cgctcagcca cttgttttgg accttcgcct gcttgaacag tcgttgttga accggctgca
    10861 gctgatgaag aaggtgttgt ttctgctgca ttgctgcttg aatcccctgc ttgataagtt
    10921 gcctcacttg aggaagtcgg tgttgtctct gctgcaatac tgctttctga cgttacacct
    10981 gtatcttgag acacagtaga ttcttccgcg attgtactag attctactgt tgaagtctca
    11041 gttgactcct cttttgttgt acttgtagaa ctttctacta ctgatgtcga agtttcagaa
    11101 gaagttgctg ctgttgcatt gttgcttgat ccatttctga tccagaaata agcacctgct
    11161 ggaatagcga tacataagac cagtaaaatc aaaaatagcg aaagaaataa tgtatttcct
    11221 ttcttttgtt gtctctgaga acttcttgat gaaacttcat catctgtatc gtagattggt
    11281 tgttcccatg gttcttgtgt ttcgttattt tctgtagatt gatgtctatc ttttctgctc
    11341 actgttttac ctcctaaact ctatcgtgtg tattatacca taggaaaaag tgtcttgtcc
    11401 taaaaaatca atgttccatg ttttttttaa ataaatttag taaaatcgtt tcccaatgaa
    11461 gttgttcagt cttagaaaaa agcttcctct ttggcagaag ttctgctttt gctccatcaa
    11521 tatcacaaca attctgcggc ttttcggaga gtttttcgcc aaaataagct aagatgaatt
    11581 ttctgcggca gttttcttca ttaatatagc gaagcatctg ctgcaggcgg aattcttttt
    11641 ctttttcctg acgtttgagc cgttccaccc aattttctgg tttttcagac tgctggatca
    11701 gctccatcca ttttttttgc agctcatcaa agggagcttg ctttgtggca tattccaaat
    11761 aaagctcaaa actttgtcgc tgttctctag aaagttgatt aaagaaataa tggatacgtt
    11821 catccccact ttgataaaaa agaattgctg cactttcttc ctgatctctg cctgctcgac
    11881 cgatttcttg tacatagttt tctagactgt ctggcaaatc ataatggacc acataacgga
    11941 tatcagattt atcgatcccc ataccaaatg cattcgtagc aatgagaaac tgcagctgat
    12001 tttttacaaa ctgctgttga agttgtcttc tttgagctgc atcaagacca ccgtgatagt
    12061 atccaactgt aaaacgttcc ctgaataaat gatacaatcg ttcgacttct ttttttgttg
    12121 cacaataaat gattgccgct ccttgtgctc tttccatgaa gtgttcaagt tcttgttctt
    12181 tttgctgcgt tttccgaaca aaaagcgaga tattttgccg attgactgat tgtctgatct
    12241 cttttggtgg tcgatcaaat agtacatgac gtatatctct tgctactaaa tctgttgctg
    12301 tcgccgttag tgccagtgtg accgggtttc ctaaaagctt tcggattttt cctaactgtt
    12361 gatattctgg acggaaatca actccccatt gagacacgca atgagcctca tctactacat
    12421 ataacgcaat attttgtttt ttcagatgct ctaaaagaga aggattagtc aacatttcgg
    12481 gactcaaaaa caaaaattta tattgcgata gatgacggag gacgtatctt ctttcagatt
    12541 gcgaaagtaa actattgaaa gcagctactc gtttctcccc tcgtttttgt aattgcgtca
    12601 cttgatcttc cattagagaa agcagcggtg acacgatgat gaccagacct tccattaaat
    12661 accccgtcaa ttgatagcaa aggctttttc ccgttcctgt aggcaaaata gcgagagtat
    12721 cttctccatc aagcaacgcc tgaataattt cttcttggcc gggacgaaat gaatcaaatc
    12781 caaaatattt ttttaattca tttattaaca ttctgtatcg cttcctttct caatagatag
    12841 atttgataca aacgaaattc tccataatct aattgtccat ctacattcca ttcgctaaag
    12901 cgccactctc gcacggaatc cttatttggt tccaaacgtt cgattgtttc tgcagagaga
    12961 atccactcgt atggaaaatc atcaaaaaac aaggcccact caataagatg atctgttact
    13021 gttcctcttt ttaaatgacg gattgccatg atctggtcaa ttgtctgacc attcataaac
    13081 atctgcttgg tgatcatcat gctttgattg aaattttgct gtaataatga atcgatcaaa
    13141 gcgtgccaaa gcgaatctgg ccgtttttcg atttgtgcta acaaaaggtg gatacatctg
    13201 ctttgttgca gataaacctc tgactcatca ttgttttttg ccagctgata aggaagcagc
    13261 cctgttcgct catttccaga taattggtta gctaaaaaat ctgctgcttc ttctggtatc
    13321 tccgaaaaca gctgcaccaa actctcatac gcagtatcaa ttaaaatttc acgtggcaat
    13381 tggctttcac taagccattt ttttagttgg aaagtataaa aaggacttgt ctctgctgga
    13441 agatagtctt gaatacccgt tgccagatta gaaattactt gaacagcaaa tttaatgagc
    13501 cgccaactag tttcccctgt tctgccatag cggtcaaaac gcaaaccagt atgttctatt
    13561 tgatgttcgg acaacatatc agcccctgca ggcgttagct ttgcttcatt ttcatttatt
    13621 tcgatccaac cttgctgcgc aattttttgc aaaatttgat aaaacttatc ctgcttcaat
    13681 tctggaaaac tgttatggat aaacaaaagt tcgtgggtaa atccataaat aagtactgaa
    13741 gtcgttcgtt ttcccacaag gagttggtaa agagaactaa tccgcagctt gtgtcctgat
    13801 gaaaatagcg ataaaataaa aacaatcata ttattctcct taagttagga tggttagaaa
    13861 tgtcactact atgtaaactt gtacctgaac gctgtattgc atgcggtctt tgtcaaatcg
    13921 aagcgccaga tattttcgat tatgatgaag aaggaatcgt tttatttgcc caagaaccag
    13981 aggctcatca acagttcgtg atgccggaag aagaaacata tgtaaaaaaa gcgtatcaaa
    14041 aatgtccagt tcgagcgatt ttattagaaa ataaaatata aatataaacg aaactttaaa
    14101 aacgtacact aagttctcta aacttgaaaa taagccggga tctgtttctc ttgttccgtg
    14161 aaatcccgac ttattttgaa agattcattt gctatctata gttaattgac tagttttttc
    14221 acggacccta taggacccat ttatattatt tcaacttttt cagccagttt tccaatgtat
    14281 tgacgatcac agtttgctgg tctgcgttag aaatttctgc ctctccatct cctttttgct
    14341 caccatagct gccaaaacct ccatgatttc cgccattgat tgatacatac tctgttcttt
    14401 ccggtaaata ctgcttagct tcttgataag cttcctgatt caatacttta tcttttgtcg
    14461 ccgtgatcga taaaacagga acagctgatt ctgtcaatgt tccttttttg tcgggataac
    14521 ttgccatgaa gaatacccct tttaaatgtt tgtctgaatg attcttattg acataacgac
    14581 ttgccattac gccacctaat gaatggccac cgatcacata atcggaattt ggaaaatctt
    14641 ctttgacctt atccgctttg tttccgccca tcactgctag atcaagtggc attttgacaa
    14701 tagcgactga aaagccgttt tttgctaaat cttgcgccca aatactataa ctttctggct
    14761 ctacgagagc accaggataa aaaataagta atggagactt tccatgattc tttttctctc
    14821 cttcaaaaaa taagtaatct tctttgtttt ttccttcttt taccaacact tgcgcttctg
    14881 acgatggttc gtacgtattt tgctgtatat agtaataacc ggctccaccg agtaatccta
    14941 aaatgatgac taagccaatt agtattcgtc taaatatttt cactttctcc caccttttct
    15001 atctgagtga cccctttttc ttaatcaagt atgattatac cttaaaaaaa taagagagaa
    15061 atgcaaactt tccagcatca aataaaaaaa gacggaatat ccatttgaat atccgccctt
    15121 tttgtatgtt tatttttatt tagcacataa acgatacaca gcttctgcat aaatttccat
    15181 tgctttatac atactactta attcccatcg ttcattcgcc tggtgcataa agtcaggagt
    15241 atcagggaac atcgcaccga atgccacaca ctggttcatc gtacgagcaa aagtagcgcc
    15301 gcctgataca aacggttcag tcatgtctcc agtaatatcg cgatatgtcc ccaatagatt
    15361 tttcactaac tcactgtcta gtggcacgta taatgaatca aggaagtcaa attcttcata
    15421 agtcaaccca tatttttctg ctgttttagt taatttagct actaaatcat ctttcttgaa
    15481 tgtaacaggg attctcatat ctacaccgat ttttgtttgt tctggtgtga tttccaaact
    15541 tgcaaagttc atcgttagct cgccggattg ttcatcttct gttttgccca cgacagcttc
    15601 accagttgca ttttcttgaa ctaattttcc taagaaattg attgggccaa aatcaaatac
    15661 ttctgaaaga gcaattgcca gacgagaaat agcattcact ccttgtgctg catctttcgc
    15721 gtggatactt ttaccaagaa cgacgatgcc ttcaccttgt tcttcgaaat cgaattcgtg
    15781 ttttttcaat gcttctttta cttcagaaag cttttctcca gagtaaggag ctgcatcagg
    15841 aacaacattc aacgctcctc cagcttttac agaaaactcg ttcgttcctg gtcctgtcaa
    15901 gtaagcttgc aataagcctt tttctgcata aattaaagga aattcagcat ctggcgcaaa
    15961 tccttgcgta attccttctt ctttttcgtt atatttttct aaacaacgcc ataaattttc
    16021 ttcatctgta ccaaagataa aacgaatacg tgtcttgaat tccacgccag catcaatcaa
    16081 agctttgacg gcatacattg ccgcaataga aggacctttg tcatcttggg atccgcgacc
    16141 aactaaccag ccgtctttta tagttggatc gaatggtttt gtttcccaat tattttcgtc
    16201 ccctgctgga acaacgtcca tatgacacaa gataccgaat aattcatctc cagagccaat
    16261 ctctgaataa ccgtaatagc cttctggatc tttaaatgtt ctaaatccta gcttttcact
    16321 gatttccaat actttatcca aagcttcaat cactttttta ccgaagggat gtccttgtcc
    16381 tgaatctgct tcatctagta cggaaggaat tcggatcaat tcgctcaatg attctaatgc
    16441 ttgttcatga tgttgttctg tcacaaattt tttcatgata ttatcctcct aatatgttaa
    16501 aaagggtcat gtcaaattcc tccgacacaa cctcttcttc tcaaataatg acgttttaag
    16561 cctgctccat cagcaagcag aatgttctta acaaatgatg aaagaaaaaa cactcactga
    16621 atggagctat tgacttgtcc acgtgcttat cgatagccgt ttatcggttt cttcattctt
    16681 atgatagacc ttgtacctgt atttgtctag gtttagaaca ttgctgctac acctaggatc
    16741 acacagatgg ctacaaataa tatcgcaatt aatttaccgg taaatttcca ccacactgaa
    16801 acatcgaaac gagcaattgc tacagcaccc attacgatag cagatgttgg tgtaattaag
    16861 tttactaaac cagatgcaga ttgataagca gtgattacta aatccttacc gacgccggca
    16921 aattcaccca taggtcccat gattcccatt gttgcagcag ctaagccgga agttgaagga
    16981 atcaagaatg acatagggat atagaaaata aatgtaataa tgatgaatac acttgaagat
    17041 aatgaactta atccttgttc gccccaatgt aagatggtgt ctgtgattaa tccattgttc
    17101 ataaccactt ggataccacg agcaacagct actacgattg ctacaccgat caaatcagaa
    17161 gcaccagcta aaaattcaga gacaaattca gactctttca tgccataagt gattgctact
    17221 acgatggaca ttactaagaa taacatagta atttctggga agtaccagtc acctaatgga
    17281 agcatgcttt tacctagaac cgtacctagt accggaaggt ttgttaacca gtttgtcaat
    17341 gattcaaaaa tcgtgaaatt cgcattcaag gttgtccaag gaatcaaact gatgatcatg
    17401 ataccaaaag taataaagaa caatacatta acatgttttt gttttttcgt gattacttct
    17461 tgacgaccga ttgcttcaat atcgaaatgc ttcatatctt ctttacgttg gttgtatact
    17521 aatgattttg ttggatcttt ttcgatcttt gatgcataac gatacacata tacaatagaa
    17581 atagctaaca aaataacgaa catcaagaat cgaagaccaa taccttcacc catcgaaatc
    17641 ccaacagctt gtgaagctac gcctgttgca aaaggattta ccgttgaagc tagacagcct
    17701 acttgagacc ctactaatac gattgctaca gctactactg tatcaaagcc tacagccatc
    17761 atgactggga tcagtaaagc atagaatgga atcgtttctt ctgccattcc gtaagtagag
    17821 ccgcctaatg caaataacag catcaatact ggaataagca ttttttcttt tcctttgaat
    17881 ttcttcacaa ctgaagcaat cccggcatct agtgcccctg ttttgttgat aatacctaaa
    17941 aatccaccaa caaccaaaat gaagaaagag attgggattg cagcaggtgt ttctttagtt
    18001 agctcccccg ctggtacacc tagcatccca ttgattggcg ccatgaaaat atcccacagc
    18061 ccttgaggat tttgttgtac tcgttcatat gatccagcga cgatgttgcc tgcatcgtct
    18121 acactatagt gcccagctgg tataaaccaa gtaagtacag cgattactgc aataataata
    18181 aataagaccg tatacgctga aggcatttga aattttttct tttttgtttg ttccatcttc
    18241 ttcttccttt cttttccgtt agagacaaca attttggctc taatgttatt ttatcaaaaa
    18301 gagtatcaga acaaaaataa taacacgcat tttgtaaaac ttttgttcaa aaagttcgtt
    18361 tttccctatt ccctcctctt ttttcaggtt tttaaagctt tcttcttttt cattgtagcg
    18421 tttccttttt tttataaaca aacattttcg tcttttaaaa ggagaagaat ttcacatttg
    18481 caaagaattg attgattttt atgcataaat caaaaaaata ctcaaaacaa acaaataaaa
    18541 cgtttacatt attgcatgtc ttgtattttt ttccaaaaaa aaagaccaag aaatttcttg
    18601 atctttttga tatttatttg actgctgtat gacgttgttc gaactgatgt tgttttcgac
    18661 ttaaccatgg aagaagttgc gcatgcagca ctaaaaagac agcacttacg atgaatcctt
    18721 tgatcaagtt gaaaggaacg atcccaatca gtacatagtt tgctaaagac atgcccagca
    18781 tctgtgttgc gttcagccct aaaaacatta agtaaagagg cgtaataacg aaataatttg
    18841 cgatactcat aaaaatagtt agagcgatgg ttcctgtaat gacacctaaa aatttgtttc
    18901 ttcctttatg ctgtttcttt acactaaaga aataataaat cgggaatgta aagatcgttg
    18961 ttgataagaa actagcagtg tctcctacca aattatccgg agaaaaacct gtagtcagca
    19021 gatgtaatac tgaacgcaag aacgctgttg caactccggc cgctggaccg aataagaaca
    19081 tgctgatcaa taccgagata tcactaaaat cgatcttcaa aaaactaaaa gccggcatga
    19141 tcggaaaagc aaaatactgt aaaactaatg cgatcgcagc aaacatcgct actgcaacca
    19201 ttttctgtac acgggtgttc ttcatttgaa atcctcctgt taggactcct cttcagcgaa
    19261 ctttttgacg tttctctcca aatgaaaaaa ctctattttc cagggaaaat agagttagag
    19321 ttatttgatc aagcgtcgtc aaataagctc tatcttcttt atccagacta tactgtcggt
    19381 atcggaattt caccgattca gctatttttt tgcaaaatag gtcgtggact ataaccaccg
    19441 gtcgggaatt tcaccctgcc cctgaagacg aaccttatat aatttttgtt tacatattca
    19501 ttatacatat ctattcaaaa aacgcaacat ctaacactta taaaaagtaa gtgttcattt
    19561 tgagtggttc agcaaactgc tgacttcttt tttgttcagt tcacggtaat ccccaggacg
    19621 taggccttgt aaagtcagat ctccgtattt ttcacgtttt agtttttgca caggaagacc
    19681 aactgcttga agcatatttt tcacttgatg attacgtcct tcgtggatcg tcaattccac
    19741 gatactgtgg tttgtcttag gatccactga taaaatttgg aatttagctg gagatgtttt
    19801 tcgtccttcg atctttattc cttttgtcaa aggtgcaagc atcgattttg tagcgatccc
    19861 ttttaccttt gcaacgtaaa ctttatccac ttcatgtttt ggatgtgtca ggcgctgagc
    19921 aaaatctcca tcatttgtca gtagcaagat ccccgaagta tcgtaatcca gtcgacctac
    19981 aggatagata cgttctttta ccatcggcaa aagatccgta acaacttttc ttcctttgtc
    20041 atctgcaaca gcagaaataa ctcctttagg tttgtacaat aaatagtaac cgtattcttc
    20101 ttgataaatt gggacttcat ccacttccac agtgtcatgt ttcccaactt ttatccctaa
    20161 ttcccttaca acttgtccgt ttacttttac acgtcctgca aggatgaatt cttctgcttt
    20221 tcgacgagaa gtgatgccag cgtgggcaat tactttctgc aatctctcca ttacaattat
    20281 tctcctttat tcattccatc aaagaataga tctggtgaca cgtcttcttc aagtgaaatt
    20341 tccatttctt gtgtatcagg cagttcttct aatgatttca aaccaaaata atctaagaag
    20401 tagccagttg ttccatataa tatcggccga cctggtcctt ctactctacc tttttcttca
    20461 atcagtttat gggcagctag acgctgtacc gctcctgaaa cctgaactcc tcgaatctgc
    20521 tcgatttcag ctcttgtgat tggctgttta taagcgatga ttgctaaggt ttcgacggcg
    20581 gcttgagata atgtgtttga tgtcccttgc gcatatcgct taagtaaggg agccagctct
    20641 tttttagtcg ttaatacata atggctttcc gtttcaaaaa tatgcaaagc tgagtgcgag
    20701 ttttgagtgt aagattcttt taattctttc actaaacttg tgacttctgc ttcgctgaga
    20761 tttaacagtc cgcttaattc ttcattactg atcccttcct cgccaacaat aaaaagaagg
    20821 gcttcaagtt cacttaattt cgtcatgttc ggtatctcct actgctggat aaagcataat
    20881 tgattcatag ttttcttttt gttgcgcaat gatctgccct tttttcatca attctagcaa
    20941 agccataaaa gtagtgacaa cttcactttt agaaggtgac tcaaaaaaag aatcgaaagt
    21001 acagccatgc gcatttcttt ttatacgctc ttcgataaat accattcgtt cttcgatcgt
    21061 tgtgtcatcg ggcgtgatcg ttttttccac tttttgagaa aatttcttac gttctaagat
    21121 tttctgcatg gcacggatca aatcgatttt tttgacttgt cctcgcttca acggcttttc
    21181 agcatccgct aacccatcga tgttcatagg ttcttttgtg taataaagac ttcgttcctc
    21241 tgctttttct gtcagtcttt cagctgcata tttatattta cggtattcta aaagctgagt
    21301 gaccaattct tctcgaggat cttcttccca taattcttca tcgtctgtgc cgaattcttg
    21361 gacaggaagc aatgtcttgc ttttgattgc catcaatgta gcagccatca ctaagtattc
    21421 tcctgcaaca gctagctcga gtgttttcat tgtatggata taattcatat attgctccgt
    21481 gatcgtagcg atcggtatat catatatatc gatttccatt tgctgaatca gatgaagcag
    21541 cagatcgaga ggaccctcga atacatctaa tttaatattg atctccgtca actcattcac
    21601 tctttcttct ttttctttgc tagccatttt ccaatgtaag gctctgtgat ttcagttgca
    21661 cggatggaga catccgggta cttttcctgt aattcatcta acatttgaaa gcccatattc
    21721 tcaccgcgat aagacggatt caaagaaatg tcatggatga tcagctgatc gttcgttgcc
    21781 cagtaaatac caataatccc ttgaatgttg gttgaacctt ctggcgtaaa gaaaaaaagt
    21841 tcataatcgt cttcagattc gtacgtatca atctccttca acaaagaagt tttacctgtc
    21901 agtttcttat ggaaactcaa tagtcccata gcaattttac ggttttcttt ccggtaatgt
    21961 gttaacatct tctcaccttc ctatgactaa gcccttggaa aatgctgctt ataaacatcc
    22021 gtcattcttt tcttcgtgat atgtgtataa atctgagtcg tagagatatc cgcgtgacct
    22081 aacaattcct gtactgtccg caaatcagct ccattttcta gcagatgtgt ggcaaagcta
    22141 tgccgcagcg tatgtggtgt tacttctttg taaatcccag cctctcgtac taactgcttc
    22201 agatttttcc agattccttg ccgagaaaga cctttcccat gattattgac aaatacatgc
    22261 atttcagatg cgtcctttac aagtaatgga cgtgcttcgt ctaagtagcg ttccagccat
    22321 tggatcgcat aatcacctaa aggaatgatc cgctctttat ctccttttcc aactgtctgt
    22381 accagtccaa gagaaagatg aagatctccc aatttcaatc caatcaattc actgacgcgc
    22441 atcccagtag catacatgac ttccaagatt gcacgatctc ttatccctaa cgtttttgtc
    22501 gtatcaggtg cttcaatcag cttttctact tctgtcagag tcaatgtact tggtaatttc
    22561 tgtgctttct tcggcgtatc gatatgttgc atcggatcat gatcagtgat acgctcttgc
    22621 cgtaaaaact gatggaatct tctgagactt gagatcatcc gagtaacagt tgctgtcgct
    22681 tttttttctt catgcaactt ctccagaaaa cggatgacta aaaagcgatc gacatcctgc
    22741 catgaagcaa ttttctgctc ctgtagaaaa tgcaagtact gttccaaatc acgttcatag
    22801 ctttgtcgtg tattggcgga caatccccgt tcaatattca agtagtggag ataatcgttg
    22861 atttgctctt tcataataat ccccctcatg cctctctatc atatcagata ccgcttcatt
    22921 ctgatagtct ttttccataa tctgagcaaa gaaaaagccg tctagacact cagacggact
    22981 ggttt
//
LOCUS       DACDIE010000043        22551 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.043, whole genome shotgun sequence.
ACCESSION   DACDIE010000043 DACDIE010000000
VERSION     DACDIE010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.043"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            264..677
                     /locus_tag="IV250_001830"
     CDS             264..677
                     /locus_tag="IV250_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAP8769113.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            1873..2418
                     /locus_tag="IV250_001831"
     CDS             1873..2418
                     /locus_tag="IV250_001831"
                     /inference="COORDINATES: protein motif:HMM:NF024252.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8769114.1"
                     /translation="MHMHIGEKIYKLRRLNNETMNDLVKQTGVSKSWLSDIESGKKQR
                     VDFNKICAIAKHYKVSLEYFRDDYLDSDEHEDIIPLTGETRVKSVKSLEQSLKYLNNK
                     QEPNLLEIKLKDTDSVPEVWYKGERLDELPKGLVDVSYHWKTDDFTNDDRGANDITIQ
                     YFSSFNDKYPDKKTIGHKRDM"
     gene            2419..2736
                     /locus_tag="IV250_001832"
     CDS             2419..2736
                     /locus_tag="IV250_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP8769115.1"
                     /translation="MKEVRPRDEIDKLYKTKRWRDLRQVVIARDFGMCQECKRRGRNT
                     RGTIIHHIVEAREDLSLFWSVDNLECICVACHNREHPERSGGKKKPKPKSHIVKMYST
                     PER"
     gene            complement(2893..3057)
                     /locus_tag="IV250_001833"
     CDS             complement(2893..3057)
                     /locus_tag="IV250_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzC family protein"
                     /protein_id="HAP8769116.1"
                     /translation="MGELYKPGEDNKPKGTYKEVGPRGGNVQGGQEVKIDPGDRLPPT
                     SQKGNKWTKK"
     gene            3159..3680
                     /locus_tag="IV250_001834"
     CDS             3159..3680
                     /locus_tag="IV250_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HAP8769117.1"
                     /translation="MPQPAKSAKLQLLNGNPNKKNTEELRKRAAAEDKLKMATDKIKP
                     PLWLDSLGKDTFEFIADELLSVDLISNPDVHTMALYSNWYSQYVSLEKQLRKLQREYK
                     LNYALAKKEAEARGEPFNEPNELIGNPLSRQMDTASRNLRSFGADLGLSPAARAKLAI
                     KMADDGGDDDDDF"
     gene            3652..4107
                     /locus_tag="IV250_001835"
                     /pseudo
     CDS             3652..4107
                     /locus_tag="IV250_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010965199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
     gene            complement(4147..4305)
                     /locus_tag="IV250_001836"
     CDS             complement(4147..4305)
                     /locus_tag="IV250_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HAP8769118.1"
                     /translation="MDKYGLKNRIRISNAIDKDLYEGLKKMSEETMVPMSKLLDKAIE
                     LLLKESQK"
     gene            4398..5159
                     /locus_tag="IV250_001837"
     CDS             4398..5159
                     /locus_tag="IV250_001837"
                     /inference="COORDINATES: protein motif:HMM:TIGR01453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP8769119.1"
                     /translation="MKKYIIYKHLNKINGKIYIGVTNDIGRRWRSGGIEYKPPKNETQ
                     HHRSFWNAIQKYGWENFDHLIIEEDLTMKEAFEKEKFYIELYDSTNKKKGYNIAKGGN
                     GGIIYKVHPKGMLGKKQSKEFSSNHSKWAKNHKNNCMTNGDVVWGVTHEHPKGMLGKH
                     HSKESIMKKKAYSGENAVTSKPIVAIERDGTKREFHSAKLCMAYYSISTCVFYRLLKD
                     GAPYVINPKANYRNKEKILAIEGIMFKHKEDTEVS"
     gene            5346..6698
                     /locus_tag="IV250_001838"
                     /pseudo
     CDS             5346..6698
                     /locus_tag="IV250_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
     gene            6714..7934
                     /locus_tag="IV250_001839"
     CDS             6714..7934
                     /locus_tag="IV250_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP8769120.1"
                     /translation="MSILDRLRSFGRAKPKASKQEYFLNDPGLIPYLVGKDEISEGIF
                     SVISRVSNVFASLPLKMIDVEFGQPDDCPAYNLLSEGPRYFTKFDFFRDVEVLRNYQG
                     NAYVQIFRNINGEVADMALVKPGACHPVIDMDSGELYYQVTATDKGSYKQVIYVHYME
                     MLHFKQPRFGGLEGTDPTKVLTNTLGYDREVRKISLSQLKGSNEGLKVKFASNMDEEA
                     KKATVKNIADFYRQNGGLLVEENGVEIERLQRELVDSKLLDTDKVSRSRIAMVYNVPE
                     HFIGNNQSSYSSQEQLNMEFLTYNLVPTVNQYEAELNKKTLSRTEKAKGYRYKFNVAS
                     LLRADTQARGQFYQIMRRGGAYSANDVRRFEDLQPINKTGMDDYHISGDLYPIDMDPT
                     LRKTTSSKSVAENG"
     gene            7969..8646
                     /locus_tag="IV250_001840"
     CDS             7969..8646
                     /locus_tag="IV250_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAP8769121.1"
                     /translation="MKKVTLSGDVVDNDTAWLYDWFGINCISPGKISAALTEAAGDEV
                     ELDISSNGGDVLAASEIYTAIRAYPGKVSGNVVSIAASAASVIACACEPLRISPTAHI
                     MIHNAWVTTSGNAEELKANAEMLSSVDESIVNAYEIKTGLDRKKLADLMAKDTWLNAQ
                     TAVAEGFADEIMFAEAPVTVLNASQPVIPKNAVTKLKNLILKAEAPQEETLLQKKLKA
                     LNGGKNE"
     gene            8643..9836
                     /locus_tag="IV250_001841"
     CDS             8643..9836
                     /locus_tag="IV250_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP8769122.1"
                     /translation="MNLEQLKNAWVEAGSKVSDLNAQLNAALVDDEKTEEDVVSLQAQ
                     VKAARAKRDGLKEQVANMEAEQVLNVKKEPLDKKDENLKNKFIKDFKAMVNGDPAIMA
                     TLTSNTDESGNAIGLTIPVDVQTTIHTLVRRFDSLQEYVNVEKVTTTSGSRVYEKWSD
                     ITPLTALDTEDGEIPANDDPALYLIKYLIKRYAGISTVTNSLLKDTAENILAWLSKWI
                     AKKVVVTRNTKILAAIDGIKAAQKKDVTDVDGIKDIVNVQLDPAIEATSMFITNQDGF
                     NVLDKVKRADGSYLLQKDVASATGYTFLGKPIKKIASRFLPNKGTQATPKYPLYIGDL
                     KEAVTLYDRENMSLLTTNIGGGAFETDTTKVRVIDRFDVQLVDDEAVVLATFTTIANE
                     TPAKV"
     gene            9851..10177
                     /locus_tag="IV250_001842"
     CDS             9851..10177
                     /locus_tag="IV250_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAP8769123.1"
                     /translation="MILDPKMDLGEIKNALKIDTDDDDVEVSRAAQAAIAYIRGAIGN
                     DKPSFYKQENETVDLINLAILQLADHYYNARSATVSGNLREYDLGFTSLILQLKASYL
                     LFVEEE"
     gene            10181..10516
                     /locus_tag="IV250_001843"
     CDS             10181..10516
                     /locus_tag="IV250_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAP8769124.1"
                     /translation="MPLIQTGNLNQRIKFVRDTTVKDEDGQVVPTSTTILTCWASVQT
                     QRLNDIKTSIGTALEGTLTFIIRYQQKSELTNDMKVRWNGKTFEIITITKGEFAKDFT
                     TIIAKEVSK"
     gene            10513..10908
                     /locus_tag="IV250_001844"
     CDS             10513..10908
                     /locus_tag="IV250_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769125.1"
                     /translation="MSVEVDATEVYKALREVKANVQRVESPALRKAGEYAQEELRQNT
                     PYWDGTKSNGKRGSYMQEHAKNHVVTSSIKNGLVEVGYDKDVSWRMHFIEFGTIKQRP
                     KGFVQKTQKQIEKQVTQIIADEVKRRLGL"
     gene            10905..11276
                     /locus_tag="IV250_001845"
     CDS             10905..11276
                     /locus_tag="IV250_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769126.1"
                     /translation="MKTAVSQVYSILNSNEKTKNIDFYTNSVPESAQTVPSLPVGRIT
                     EISGNYEDFASNNPLTIQFNVQVDVWVSTMKEVDAFYFALDEVMRGNGWQCAYTEQTD
                     DEDLEGAKRIIKRYVANISLN"
     gene            11292..11852
                     /locus_tag="IV250_001846"
     CDS             11292..11852
                     /locus_tag="IV250_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP8769127.1"
                     /translation="MATVGFESVIFGVKTGAGGTLKELVADKSKGGAIEAKITGLGAT
                     SNTTYASNVPFFIASKGVSSPKVTLDVADLMDNGIYSEIIGAKTVDGANVIGSETEAP
                     YVSVVMVTANKEGKRLFMGLTKGKFSHPDIDMKTAEDKGVELQTDSIEGEFISDERGY
                     VYLTAVESEGMTLQKFKDLVNNKEGE"
     gene            11977..12288
                     /locus_tag="IV250_001847"
     CDS             11977..12288
                     /locus_tag="IV250_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769128.1"
                     /translation="MIELQLKLDGKKKTFKQQDISARAMRECIKFYEKAEKADLTDLE
                     AIDSMIAITADIFQDPAVTFDAILDGLTASELVPALESVFEQINELGNNEKKQTASKK
                     K"
     gene            12312..12503
                     /locus_tag="IV250_001848"
     CDS             12312..12503
                     /locus_tag="IV250_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769129.1"
                     /translation="MDQIYKDLIEAGWTMRDVDEADYHYLLHLFGEVESGEEYVDGAD
                     FIKQFLSAEDLVKLEEGGK"
     gene            12503..16359
                     /locus_tag="IV250_001849"
                     /pseudo
     CDS             12503..16359
                     /locus_tag="IV250_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922442.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
     gene            16360..17232
                     /locus_tag="IV250_001850"
     CDS             16360..17232
                     /locus_tag="IV250_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769130.1"
                     /translation="MRTVLLRNRINEEIDLSTEDYFATGLSNMGFEVKKEHVGQWGNF
                     RESSESVEISEFQSSVIISVHGFREKELYNSLVQFLSEGPFELEFAFDGETMVRRCSL
                     KSLSKTEIDPKTSLLTDTLELYFTSNWYSVKREKLIQRPNVVKTRGKVFPYKRSYIYT
                     QNLWEKKGVFKFNNNSVYLTNSKERMSPLKIRVIGKCSNPYWEVIQNSQIIATDGYFI
                     DMTETQTLEVSSLFEDTTAILKDIAGVESSVYQQQDYTKTNFVQAPTGEFSIVFHVGG
                     ADVEIELYEERDLF"
     gene            17229..18278
                     /locus_tag="IV250_001851"
     CDS             17229..18278
                     /locus_tag="IV250_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769131.1"
                     /translation="MILAVTLFHRDLMLYNEHLFSNSFEFGVDEINEEASSFTMDKYV
                     PVKTGDFLLAKYIPSGKFAYFGVITSQEDEKISCKSLLSLADSEIPTARVSGDNYEEH
                     IRRLIEYYLLNDPTKQLKDILDVKAESATSHSYQATDTNKHKLSAYILNGFKKYNVKW
                     YFKGIQNRKIYTGIRAVNESIYIKDNSSEFSDWDVFVQAPGAGNENKLLIVDKAMKDI
                     EKPIILSTWYLDEENNLTQDGSKENITKPTVNLVNIYDQTAEDKASYEDVAKSELKGN
                     TYSHEIKVNVVRNAKNLNVETIETGMFATISYKGKIYKSVLTAWRISSDKEFVELTFG
                     NIRSRFMDYFEDNGG"
     gene            18282..19106
                     /locus_tag="IV250_001852"
     CDS             18282..19106
                     /locus_tag="IV250_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769132.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERV
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            19124..21184
                     /locus_tag="IV250_001853"
     CDS             19124..21184
                     /locus_tag="IV250_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HAP8769133.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDQTNQQIGDLGKLKKMYSNSIDFGGYDYSGNPNL
                     LSKLSYDLIENQNTSAGTLSKGENSFKYNKISAEVEGGVELYYKRRGIANWLPSNKTL
                     VMTVKLRAGADYSPVDGKLILIRYRYVDSGTGKIVLDLPINSNSITQEWKEFSITGTT
                     PTFSPQAYHPWIQFRAQDGVLGEIEMSYDIKIEEGSTATPYQPNLLDDSYWLGKTPLG
                     ENLITNAKFPIITRSNPISGFDISEELIIGETYTVSLKGTKPATKEFHLYYGEANYQQ
                     LQYQATLLPVEGLTDVWSATFTARNTSETTNLLRVALWQKPNSATYDTVQIDWLKIEK
                     GDTRTPNISQFKYFGEGLKDSNNPNDYSWDVTPEYTEKSLNNTVSLTEPQSIEGLKNF
                     EDGIQSKGKSVLTSDDNKYEVVTLTVTNGNTGSAKLYREGKTVTIYFFALNGKSSGGN
                     DSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSDYTFAI
                     TYTI"
     gene            21347..21793
                     /locus_tag="IV250_001854"
     CDS             21347..21793
                     /locus_tag="IV250_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769134.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            21795..21932
                     /locus_tag="IV250_001855"
     CDS             21795..21932
                     /locus_tag="IV250_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAP8769135.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADRIISKEESA
                     S"
     gene            21967..22209
                     /locus_tag="IV250_001856"
     CDS             21967..22209
                     /locus_tag="IV250_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP8769136.1"
                     /translation="MKEEALQDVVERLVRIETKLDNYESLREKADSAKDLADKAYSVA
                     LNNAEDIKEMKANNKWSWGYMIGLGITIIGYFLTKL"
     gene            22225..22422
                     /locus_tag="IV250_001857"
     CDS             22225..22422
                     /locus_tag="IV250_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP8769137.1"
                     /translation="MILPDKYYQVIKWTVLTVLPAASVLVATLGKAYGWNGTDMTVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            22433..>22551
                     /locus_tag="IV250_001858"
     CDS             22433..>22551
                     /locus_tag="IV250_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP8769138.1"
                     /translation="MKKKIILSLSLIMALFLLPINGFAYTINNEFNLGVNEGSS"
BASE COUNT         7815 a         3825 c         5018 g         5893 t
ORIGIN      
        1 aaaatgcgac gataccaata caaacgggaa tccaaaataa cagcatgctc cacatagtca
       61 ctcaaccatc tcctatacgc tttattatca atagataata tcatttttcg taataaagat
      121 aaaaaaataa gtaaggcttc tcattaaaat ataaaaaaga cagccgacca ctgactgcct
      181 atataagagt attgaaataa aaagctgctg atataataaa ttccacaagt ttattatatc
      241 acataaagga gcggtttgac ttgatgcaat tgttacgaga ggtagatttc aaacagacaa
      301 gatgtaatgc gagagatgtg ctgaagaact ttcggcgttt ggagcggatg gcaggtcgct
      361 ctttgataga tattaagtcg ccgattatta cggatatgcc gaaggcgccg aagcacggca
      421 ataaggcaga agacgcaatc attcagatga tggatataga agcggagaga gacgcgattc
      481 tagcagcctt gatggctctt agtctgatta gtcgtcagat actctactat agcttttgtg
      541 tgccagatag cttctcaaac tacagaatta gccgtgaagt gggttattca gaaagaagta
      601 tacaacggat gaagtcggaa gctctaatag agtttgcaga agcatataaa cacggaagaa
      661 taattgctta taaataattt ggcggctttt tggcggaatg atggcggttt ttagctattt
      721 accagtgata ttatggtagt gtcgaaagat tagtgatagg tctaagacaa aataataata
      781 aaaggaacat cgttttatta ttgtttcaca attaagcttc gatagacagc aacggaaata
      841 ttaagaataa ggatgtgaat tttaactcct tctaaattgt tcttattatc tatcatccgt
      901 tgctgtctat tgttttttta attattcaca cgataactaa aggtgagcga agagaaatga
      961 ttccattaat aatttcaatt tttgcgcttt gtcttaatgt ctatatgatt ggatttaaaa
     1021 atgggcaaaa taaaagatag tagtaactaa gaaagaaatt tttatattgt cactgtggcg
     1081 gaaagggtag acgctaagca tgtgtgctag gtcaatgctt cggcaactat gcaatgttcg
     1141 attcattgcc agtgactttg gcaaccgagg catcggcggt ttaaaaatat aggggtgcgc
     1201 aatttcgtac gcgttttgtg catcgtgtag gttgctatta catattagat cactcgttga
     1261 gtggtctttt tattttacat aaaggaggta acaacgatgt atagaccaca atacttagaa
     1321 cagaagtatg aagtaatcac tgtgcaaaac ggtaacggtg agatagtacg aaagtataga
     1381 agaccaataa agagcgatac atataaacga aaggaaaaca atgaagttat ttcttttcgc
     1441 agaaggagaa aagccaaatg agaaactact ggtatgtatc gctaactaat gaatatcctc
     1501 gaaccattga tgattgttca gtgcgtgttg tgcgttctgt acaaatcaaa gggaagtact
     1561 ctattgtcga aatgctaaga gaagctacac caaacgaagt ggataaatgc aagctgatat
     1621 attgcggtca tggctattgg aaagacgagt atatccaaca aaacattgag aggtggatag
     1681 atagatgagt tacctcgaac atttgaaacg ttgctacatg cattctaaga ataaacttcc
     1741 tgacagctac actgtagatg atgttgctat acatgtattg aagactgaga gccatagcag
     1801 tcctgatggt agcagtaagg agcagacgct cgcgtggttc aagttcttta agtggattaa
     1861 ggaggaagag taatgcacat gcacatagga gaaaagatat ataaattgag acggcttaat
     1921 aacgaaacaa tgaacgactt agttaaacaa actggagtgt caaaatcatg gttatctgat
     1981 attgagtctg gaaaaaagca acgcgttgat tttaataaga tatgtgcaat agccaaacat
     2041 tataaagtta gcttagaata ctttcgggat gattatttgg atagtgatga acacgaggat
     2101 attattcctt tgacaggcga aacaagagtt aaatcagtga aatcgcttga acaaagccta
     2161 aagtatctga ataacaaaca agaacctaac cttctagaga tcaaactaaa agatactgac
     2221 tcagtaccag aggtttggta caagggtgag aggttggatg aattgcctaa aggattagta
     2281 gatgtctcgt atcattggaa gactgatgat tttactaatg atgatagagg agcgaacgac
     2341 atcacgattc aatacttttc tagctttaat gataagtatc cagataagaa aacaatcgga
     2401 cacaagagag atatgtaaat gaaagaagtt agacctagag acgaaataga caaactatac
     2461 aagaccaaac gatggcgaga cctaaggcaa gtagtaatag ctagggactt cggcatgtgc
     2521 caagagtgca agcgtcgagg gcggaacaca aggggcacga tcatccatca catagtcgag
     2581 gcgagggaag acctgtcact gttctggtcc gtagataacc ttgaatgtat ctgtgtagct
     2641 tgtcacaaca gagagcatcc agagagatca ggcgggaaga agaaaccaaa acctaaatca
     2701 catatcgtta aaatgtattc aactcctgaa agataagttt gcagcgaaat gaaggtagcc
     2761 cccctactct aaaagattaa agagtaaggc ttgagaagaa cggtgctgtc cttccttcgt
     2821 aaaaagaccg cttttcaagt tttttggaga aaaaggaaaa agccgaccaa tttaagccgg
     2881 ctttggacga agctatttct tagtccattt gtttcctttt tgagaagtag ggggtaatcg
     2941 gtcgcctgga tcaattttta cttcttgtcc gccttggaca tttccaccac gaggacccac
     3001 ttctttgtag gttccttttg gtttgttgtc ttcgccgggt ttatagagtt ctcccatagg
     3061 aatccctcct taacaaattt cggcacagca ctgccgataa cctaattata aggattgtga
     3121 taacgatttc aatctatctt ttgaaagaag gtgatattat gccgcaacca gcgaagagtg
     3181 caaaattaca attattaaac ggaaacccaa ataagaagaa taccgaagaa ctccgcaagc
     3241 gagcggccgc agaagacaaa ttaaaaatgg ctactgacaa aatcaaaccg ccgttatggc
     3301 tagattcgct aggaaaggat acctttgagt ttatcgccga tgaattgctg tctgtggatt
     3361 taatcagtaa tccggacgtc catacaatgg ctctatactc caattggtat tcgcaatacg
     3421 tttctttaga aaaacagctt cgaaaactac aacgagagta caagttgaac tatgcgcttg
     3481 cgaaaaagga ggcagaggcg agaggtgagc cgtttaatga acctaatgaa ttaattggta
     3541 atccgctctc tcggcagatg gatacagcgt ctcggaatct ccgttctttt ggcgctgatt
     3601 taggactatc accagcagcc agagctaagt tagctattaa gatggctgat gatggtggtg
     3661 atgacgatga cgacttctaa tattttggat atgtcttaca cagaacgtgt ggactattgg
     3721 caaagctatc ttgaggagca agcttcttgg ggtggctttt taaaatgtcc atatccggaa
     3781 ttgttaacta cttggtatgc ggaacgatta atcgatggaa gcataccagc cagcaaagaa
     3841 aatattcaag ctgctaaacg gcatatgcgt gatttgcagc gccaaggaac agatgatttt
     3901 ccttggatct ttgacgaaga aaaaggtcac cggcctatta gatatatcga aaaaaaatgt
     3961 aaaccaactg aaggcgactt tggttcgttt gttttgcaac cttggcagca tttcataatt
     4021 ggatccatgt acggatgggt acatcgtgat acaggagagc gtcgcttccg cgaggctctt
     4081 atttttgttg gacgtaaaaa cggtaagttt tgccgtttaa aaatcgggca aaatcggtga
     4141 aaacctttat ttttggcttt cttttagtaa taattcgata gctttatcaa gcaatttaga
     4201 cataggaacc atagtttctt ccgacatttt ttttaatcct tcgtataaat ctttatcaat
     4261 tgcgttagaa attctaattc tgttttttag tccgtatttg tccatgtgtg acactccttt
     4321 ttattttact ataacatccg attccaccgc ttgcaactac catcaattga tggtagtata
     4381 taaaagaggt gagtagattg aaaaaataca ttatttacaa acacttaaat aaaataaatg
     4441 gcaaaattta tatcggtgtc acaaatgata ttggcagaag gtggaggagc ggtgggattg
     4501 aatataaacc accaaagaac gaaacccaac accacagaag tttttggaat gcaatacaaa
     4561 aatacgggtg ggaaaatttt gatcatttga ttattgaaga agacttaacg atgaaagaag
     4621 cttttgagaa agaaaagttt tatattgaac tttatgactc aactaataag aaaaaaggtt
     4681 ataacatcgc aaaaggcgga aatggcggaa taatatataa agttcacccc aaaggaatgc
     4741 tagggaaaaa acaatctaaa gaattttctt caaatcacag taaatgggca aagaaccata
     4801 agaataattg tatgacaaac ggtgatgttg tatggggtgt tactcacgaa cacccaaaag
     4861 gcatgttggg aaagcaccac agcaaagaat caattatgaa aaagaaagca tatagtggtg
     4921 aaaatgctgt aacctcaaaa ccaattgtcg caattgaacg ggatggaact aaaagagaat
     4981 ttcacagcgc taagctttgc atggcttact attcgattag tacatgtgtt ttttaccgac
     5041 ttttaaaaga tggcgcccct tatgtgataa atcctaaagc gaactacaga aataaagaaa
     5101 aaatattagc tatcgaagga attatgttca aacataaaga agataccgag gtaagttagt
     5161 agattgcgaa aggttactaa ctaccgtaac gcgtaggagt tgaataaata taatgctccc
     5221 aagagtgtcc gacagcagat aatttgctga aaatgtacgc taaactgggc cagaactgac
     5281 tgaccgatga aaatgagggt gacctccaga gtgcgagata aaaagctcgc agataataac
     5341 aatcgaaaac atcactaatt tcaggccttt ccacatacat ggtcgcttat gatgatgaac
     5401 aaggcgccaa cgtttacgta ttggcaaatg ctcgtgatca agcaagcttg ttgtttgata
     5461 aggccgcaga aatggtcaaa caatcgccgg cgctctttaa gaaatttggt aagcctaaac
     5521 gatcaagtat taattatgct cccgcctttt ctaaaatgga accacgcgcc tcagatagcc
     5581 ggaaattgga tgggctaaac actcactttg gtatttttga cgagatccac gagtttacga
     5641 attacaatct gatcaacgtt atcaagaaat caagaggaac cagaaaacag cctctgatag
     5701 tttatatcac aactgctgga tatgtattag atggtccgtt gatgtcttat tttgagcaag
     5761 gtgtggactg tttggaacat ttggaagatg acatcgatga acggactttc tattatctgg
     5821 caaaacttga cagtgcggaa gaggctgatg acccaagatt atggatcaaa gccaatccga
     5881 atatttgtct aatgaatttt gttggcatgc tagatgacta tgttaaggat aaaaaagatc
     5941 caaaagaata tgctgactgg attaccaagc aatttaactt gttttccgat atcgatgagc
     6001 tgtcatttgt cgatatgcct accattaaac gaaacaataa aaccatcgat attgaaacgc
     6061 tcaaaggtaa gaagtgtgtc ggtggttttg acttgtccga aacggaagac tttaccgcag
     6121 ccgttttaga atttccgctt gaaacaggcg aggtattcat tttgcaacac acatggatcc
     6181 cacaagctag atttgatcga gataacaatc aagagcgtat caaagcgtgg gagaaggtgg
     6241 gagatctaac gattattcct ggtgattacg tcaattatga atacgtctta aattggtttg
     6301 tagaaaattc gaaaatctat gacattgtaa aaatcaatta tgacaaggcc aaggcgctac
     6361 gattaaataa agaactagaa aatgcaggat ttgaaaccaa cgagattcgg caagggtttc
     6421 tatcattagg tgggccaatg caaaacttca aggaaatgct attggacggt aaggtgattt
     6481 tcaacaattc caagctttac cgatggtatc tatccaacgt caagctggtg atggatcgca
     6541 actcaaactg gatgccgtct aagcagtcca agagtagaaa aatagatggt tttgcagcaa
     6601 gtttgaacag ccacgccgaa gtgttgaata tgttggttaa tcctgtcgga accgggaaag
     6661 taacctatta ctcgatttcc gatttaatga atatgtaaga aaggtgtgga ggaatgagta
     6721 ttttagatcg tttgcgttct tttggccgag cgaagccgaa agcgagcaaa caagagtatt
     6781 ttttgaatga cccgggattg ataccgtatt tagtcggaaa agatgaaata tcagaaggga
     6841 ttttttccgt aattagccgt gtatcgaacg tttttgcgtc tctccctctc aaaatgatag
     6901 atgtggagtt tggccaaccg gacgactgtc ctgcatacaa cttgttgagc gaaggccctc
     6961 gatattttac aaagtttgat tttttccggg acgtggaagt tttgagaaac taccaaggga
     7021 atgcgtatgt gcagattttc cgaaatatca atggagaagt agcagatatg gcgttagtaa
     7081 aacctggtgc ttgccatcca gtgattgata tggatagcgg ggagctttac taccaagtaa
     7141 ctgcgactga caaaggcagt tacaagcaag ttatctatgt acattacatg gaaatgcttc
     7201 actttaaaca accgaggttt ggcggcttgg aaggtacaga ccccacaaaa gtattaacga
     7261 ataccctcgg atatgatcga gaagtccgaa aaatctcttt aagtcagctt aaaggaagta
     7321 atgaagggct aaaagttaag tttgctagca atatggatga agaagctaaa aaagctacag
     7381 ttaaaaacat tgctgatttt tatcgacaaa acggtggact acttgtggaa gaaaacggtg
     7441 tagaaatcga acgtttacaa cgagagctgg tagacagcaa gcttttagat actgataaag
     7501 tatctcgctc cagaatcgct atggtctaca atgtgccgga gcatttcatc gggaataacc
     7561 agtcgagtta ctcctctcag gaacagctca atatggaatt tttgacgtac aatctagtac
     7621 cgaccgttaa tcaatatgaa gcggaactaa ataagaaaac actatcgaga accgaaaaag
     7681 caaaaggtta tcgatataag tttaatgtcg caagtttgct aagagctgac actcaggcaa
     7741 ggggacaatt ctatcaaatc atgcgacgag gcggagcata ctcagcgaat gatgtccgaa
     7801 gatttgagga cttgcaaccg ataaataaaa ccggtatgga tgattaccat atttccggag
     7861 acctatatcc aatcgatatg gatccaacat taagaaaaac aacctcgtct aaaagcgtag
     7921 ccgaaaacgg ttaggttttt ttagtttgca ccgaagggag gtggaaaaat gaaaaaagtg
     7981 acgttaagcg gcgatgtcgt ggataacgat accgcgtggc tttatgactg gtttgggatc
     8041 aattgtatct caccagggaa aatttctgcc gctcttacag aagcagcggg ggatgaagta
     8101 gaacttgata tctcatcgaa cggtggggat gtcctagcgg caagcgaaat atataccgct
     8161 atccgtgcct atccgggaaa ggtatccgga aatgttgtga gtattgcagc aagcgctgca
     8221 agtgtaatcg cttgcgcttg cgaaccgctt agaatctcac ctacggcaca catcatgatt
     8281 cataacgcat gggtgaccac tagtggcaac gctgaggaat taaaagccaa tgcagaaatg
     8341 ttaagcagtg tggatgagtc tattgttaat gcttacgaga tcaaaacagg actcgatcgg
     8401 aaaaaacttg ctgatttaat ggcgaaagat acttggttaa atgctcaaac agcagtagca
     8461 gaagggtttg cagatgaaat tatgtttgca gaagcaccag taacggtact caatgcctct
     8521 caaccggtta ttccaaaaaa cgcagtaact aagttgaaaa atttaatact caaagcggaa
     8581 gcaccgcaag aagaaacact cttacagaaa aaactaaaag ccttaaatgg agggaaaaac
     8641 gaatgaattt agaacaatta aaaaatgcgt gggtcgaggc gggaagtaaa gtctctgact
     8701 taaatgcaca actcaatgca gcattggttg acgatgaaaa aacagaagaa gatgtagtaa
     8761 gtttgcaagc acaagtaaaa gcagcacggg ctaaacggga cggattgaaa gagcaagtgg
     8821 caaatatgga agccgaacaa gtcttaaacg tcaaaaaaga accattagat aaaaaagatg
     8881 aaaacttgaa aaacaagttt atcaaagact ttaaagcgat ggtcaatggt gatcctgcta
     8941 ttatggctac tttgacatct aatacggatg aatctggtaa tgctatcgga ttgactattc
     9001 ctgtagatgt gcaaacgact attcatactt tggttcgtcg gtttgactct ttgcaagaat
     9061 acgtaaacgt tgaaaaagtg accactacca gcggttctcg cgtttatgaa aaatggtctg
     9121 atattacacc gttgactgct ttggatactg aagacggtga aatcccagca aatgatgatc
     9181 ctgcacttta cttgatcaaa tacttgatca aacgctatgc aggtatttcc acagtaacta
     9241 acagcttgct aaaagatact gccgaaaaca ttttggcatg gttgtctaaa tggatcgcga
     9301 aaaaagtagt tgttactcgc aatacaaaaa tcttggcagc tattgatgga atcaaagcgg
     9361 cacaaaagaa agatgttaca gatgttgatg gaattaaaga tatcgtaaac gtccaacttg
     9421 atccagctat cgaagctaca tctatgttta ttacaaacca agatggcttc aatgttttag
     9481 ataaagtgaa acgtgctgat ggatcttact tgttacaaaa agacgtagct tctgcaactg
     9541 gatatacctt cttaggtaaa ccaatcaaga aaattgcttc tcgtttcttg ccaaacaaag
     9601 gtacgcaagc tactcctaaa tatccactgt acattggtga tctgaaagaa gccgttacat
     9661 tgtatgatcg cgaaaacatg agcttgctga caacgaatat tggtggtgga gcttttgaaa
     9721 cagacaccac taaagtacgc gtcatcgatc gcttcgatgt gcaactagtt gatgatgaag
     9781 cggttgtttt ggctactttt acaactattg cgaacgagac accggcgaaa gtttaaggag
     9841 ctgattcctt atgattcttg atcctaaaat ggatttaggc gaaatcaaaa acgcactaaa
     9901 gattgatacc gatgatgacg atgtggaagt aagccgtgcg gcacaagctg caattgcata
     9961 cattagaggg gctatcggga atgataagcc ctctttttat aagcaagaaa atgaaacggt
    10021 tgatctgatt aatttagcta tcctgcaatt agcagatcat tattacaacg ctcgatcagc
    10081 aaccgtgagt gggaacttgc gagagtacga tttaggtttt acaagcctaa tcttgcaact
    10141 caaagcaagt tatttgcttt ttgtggagga ggagtagcgt atgcccctta tccaaacagg
    10201 aaatttaaat caacgcatca agtttgtccg agatacgacg gttaaggatg aggacgggca
    10261 agttgtcccg acttctacaa ccattcttac ttgctgggca agtgtgcaga cacaacgcct
    10321 gaacgatatt aagacgtcga ttggtacggc tttggaagga acactgacgt tcattatccg
    10381 ctaccaacaa aaatcagagc tgaccaatga tatgaaagtg cgttggaatg gaaaaacgtt
    10441 tgaaatcatt acgattacga aaggcgagtt tgcgaaggac ttcacgacaa tcattgcaaa
    10501 agaggtttca aaatgagtgt agaagtcgat gcaaccgaag tgtacaaagc gcttagggaa
    10561 gtaaaagcaa acgttcaacg agtggaaagc ccagcactta gaaaagctgg ggagtacgct
    10621 caagaagagt tacgacaaaa cacaccttac tgggatggaa cgaagtcaaa cggtaaacgt
    10681 ggttcgtata tgcaagaaca tgctaagaac catgtggtta caagctcgat aaaaaacgga
    10741 ttggtagaag tcggctatga caaagatgtt tcttggcgga tgcactttat cgagttcgga
    10801 acaatcaaac aacgtccaaa aggtttcgta caaaaaacac aaaagcaaat cgaaaaacaa
    10861 gtaacacaaa tcattgctga cgaagtaaaa aggaggctag gactttgaaa acggcagtat
    10921 cacaagtcta ttcaattctg aatagcaatg aaaaaacaaa gaacattgat ttttacacca
    10981 atagtgttcc ggaatcagct caaacagtac ctagccttcc agttggcaga attacagaga
    11041 tatccggcaa ctatgaagat tttgcaagca acaatccttt gaccattcaa tttaacgtac
    11101 aggtagatgt atgggtgtca accatgaaag aggttgatgc cttttatttt gcccttgatg
    11161 aggttatgag ggggaatggt tggcaatgcg catacacgga acaaacagat gacgaggact
    11221 tggaaggtgc aaagcggatt atcaaacgat atgtagcaaa tatttcacta aattaaaagg
    11281 agagaaaata gatggcaaca gtaggattcg agagcgtcat ttttggcgta aaaacaggtg
    11341 caggcggcac tctaaaagaa ttagtagcag ataagtcgaa aggcggagcg atcgaagcta
    11401 aaattactgg attaggcgca acttctaaca caacatacgc ttcaaacgta ccgttcttca
    11461 ttgcaagtaa aggggtttcg tcgccaaaag ttacgcttga cgtggcagac ttaatggata
    11521 acggcattta cagcgaaatc attggtgcta aaaccgtgga tggtgcaaat gtaattggtt
    11581 cagaaactga agcgccttac gtgtcggtag tcatggttac agcgaacaaa gaaggaaaac
    11641 gcttgttcat gggattgaca aaaggaaaat tcagtcatcc agatatcgac atgaaaacag
    11701 ctgaagacaa aggggtagaa ttgcaaaccg attccatcga aggggaattc atttctgatg
    11761 aacgtggcta tgtatactta acagccgtag aatcagaagg aatgacctta caaaaattca
    11821 aggacttggt aaataacaaa gagggggagt agttaaccct gcatctacac caacgacaga
    11881 tacagggaca ccaaaagaac cagaaccaaa aattgataca caaggttagc catttttggc
    11941 tagccttatt ttttgtaaaa acaaggagga aaacaaatga ttgaattgca attgaaactt
    12001 gacggaaaga aaaaaacatt caaacaacaa gatatttccg cacgtgcaat gcgtgagtgt
    12061 atcaaatttt acgagaaagc ggaaaaagca gacctaactg atttagaagc gattgattca
    12121 atgattgcaa ttacagcaga tattttccaa gatccagcag ttacatttga tgctatttta
    12181 gacggtttga ctgcgagcga gttagtaccg gcattagaaa gtgtttttga acaaataaat
    12241 gaactgggaa acaatgaaaa aaagcagacg gcgagcaaaa agaaataagt ttttctgaag
    12301 ctaggaaagc aatggatcaa atctacaaag atttaatcga agcaggttgg acgatgagag
    12361 atgtggacga agccgactat cattatttgt tacacctttt tggagaagtg gagagtggcg
    12421 aagaatatgt agatggtgct gatttcatca aacaattttt atcggctgaa gacttagtaa
    12481 aacttgagga aggaggtaaa taatggcagg aaaaggacaa ccggcaggaa atatcaagct
    12541 agggattagt ttagatagca ctagttttgg taacacgctg gacgaaatca atgcgaaagt
    12601 caaacaagct gagtcgaata tgcgtgccaa tctaaaggct tatgattcag caggacgttc
    12661 atacgaagca cttagtcaaa agacgaaaga cttgtctacg gttatggaag ggcaaaacgc
    12721 caaagtaaga gaattaacaa agcgccgtga tgaagcgatt agcaagtatg gcgaggaatc
    12781 gaaacaagtt gctaacctta acacacagat aaacaatgct accgcaaaat ataatgctta
    12841 cagtcgccag ttgaacgaca caaaaaaaga attggtgtat tccaaaacag ccgtcaatga
    12901 tttatctaat gaaatcaaag aaaatgaacg acaaatgaac gccgaagtaa aagcgttgaa
    12961 agccgctggt gatgaatctg gtgcttttga agcaaaacaa aaagggctag ccaaacaaac
    13021 ggaattatcc gagaaagcta tcgaagaaca gcgcaaagtt gtgaaactga tggctgatga
    13081 gtttggcgat tcagcaaatg aaaccgaaga tgcaaaaagg gcattagaaa agttagaacg
    13141 acaaagccaa atatctagca ggcaattaga agcactcaaa agctccagcg atcaatcagg
    13201 aaaagaaata gaagattttg gcgacaagtc cacaaggtca gctaggaaac tggacggact
    13261 aaaagacaaa ttaagctcgc taaaaagcgc attttcgttt ggtgcagttg ctggattagc
    13321 gcataacgct attagcagtg tagtaagtgg cgtgcaaggt ttggttggcg aagcagtaaa
    13381 cgcatcggat tcattgatga agttttccaa aaccatggag tttgctaact ttgggaagtc
    13441 acagatagaa agctcgaaaa aagaaatgaa agactacgcc gataagacgg tttatggttt
    13501 agaagaaatt ctgaacacaa ccgcacaatt ggcatctaat gggattccta actatacaga
    13561 actaaccaag gcggcaggta acttgaatgc cgttgcaggc ggttctagtg atacattcaa
    13621 atccgttgcc atgatgctaa cgcagacggc aggagctggg aaactaacaa ctgaaaactg
    13681 gaatcaatta gcagatgcga taccgggtgc ttcaggattg ttacaagacg ctatgttgaa
    13741 aaacggagct tatacaggaa acttccgtga tgcaatggcg caaggtcaaa tcacttccga
    13801 cgagttcaac caagcaattg tacagttagg tatgaatgac ggagcagtta aggcagccac
    13861 ttctacagac acattgagcg gttcttggga gcagatgaaa tccactgtaa taaatgggct
    13921 acaaagtatt atagaaaaaa taggcgttga aaatatcact ggtttcatca acagagtaac
    13981 aaaagggatt gaaaattcta ttcctaaaat tactcaattt ataggttggt tgagagatat
    14041 tggaacgtgg atcgttgaaa atagagagcc actaacatgg attgtcggaa tcataggcgg
    14101 aattacatta gcagtaaaag cattgaacgt agcaagtatg ttgctggcaa ttactggcgg
    14161 aacattggca gccccttttg tggcgattgg tgtagcatta ggcgcactag caggtgcttt
    14221 ggtgatagct tatacaaaat ctgaaacatt tagaaatata gtcaatgcgg cttttacagc
    14281 tgtgaaaaac gtagttatga gcgttatcaa taatttggtg gaatactaca aaatgttgtg
    14341 gagcgtgttg cagtggcttt gggaaaaaat aaaagaatgg gcttcatgga ttggcaataa
    14401 attcattgaa atgaagaaca gcgttgtgaa tacggttcag aacctatgga atggcgtgaa
    14461 aaacttcttc agcaatggcg ttggagatac gtggaataag gtagtcggtt gggtaaaaaa
    14521 cattttcaac aaagcaactg aattgaagaa caacgtttct gatgtaatcg gtaacctgtg
    14581 gaatggtatc aaagacacat tccgtagagg tatcgatacg gtattcaatt ggttttcaga
    14641 actaccaacg aagatgaaga atgccattat tggcggtaaa aacgccattg ttgatgcgtt
    14701 caaaagtatt ttcaacgcag cacttaaagc gataggtaaa ccagttaacg caatcatcca
    14761 tggagcttca tgggtactag aaaaattggg cgcagaacct ctacaagaat gggatgtacc
    14821 acaatacgct acaggtacac cagcaggtgg gcacccaatc aatggtccaa tgatggttaa
    14881 tgatggacgt ggagcagaaa cagttatcac accagatggt agagccttca ttcctaaagg
    14941 acgcaatgtt gttctaaacg caccaaaagg aacgcatgtc ttgacagcag aagagaccgc
    15001 gcaacttcaa ggttctaaag ctcctaagta tcgttacaaa aaaggtacta acttctttgg
    15061 taacatgtgg gatagtgtga aaaaggttgc tggtaatgta ggtcacacaa ttaaaaacgt
    15121 agtaggtgac gtgtgggact ttatttcaga cccgggagcg ttagctagaa aagtacttgg
    15181 ggggttagat gtattaggtg gattgacaaa gtatccatta gaagtaggta aaggcatcct
    15241 atctaaagca acaagtgcac tgactgaaaa aatcactggg ttgttctcat ctggtaactt
    15301 agatacctcc gtaggaacaa atggcgtcta taaatatttg gctgatgttg ctaagtctgt
    15361 gatgaagaaa ttcccaggat ttgtggcaac tagtgggtat agacctatag accaggtgac
    15421 ccttattcac atggtaaacg taatgccatt gatattgcat taccaggcgt cacaggaggc
    15481 tcacctagat acacagaagc agcaaactac gcttttgaca aattcgcttc caaaattggt
    15541 tacgtaatca ctaatgggaa agttcgtgac cgttcaggac aatcaggtca accagcaact
    15601 ggtgcatggg agccatggcc cgatggagat cactatgatc atgtgcattt aaacggtgtg
    15661 aaagacccac aaaacactca aatttcagga gatagcgtgg gaggcagtgg ggtagaaaga
    15721 tggcgcaatg tagcaattag agcgttgaaa atgaccggtc aatacagtac tgcaaactta
    15781 aatgcattac taaatcaaat gcgtacagag tcaaatggta atcctaatgc agttaacaat
    15841 tgggatatta acgccaaaaa tggaacacca tcaaaagggt tgctccaagt gattgaccca
    15901 acattcagac agtatgcaat gccaggattc aacagcaata tttttgaccc actatctaac
    15961 atcttagctt caatcagata cgcactatca agatatggct cactaacaaa tgcctatcgt
    16021 ggagttggtt acgcaaacgg tggaattgta aaccaacatc aaattgcgga aatcgcagaa
    16081 ggaaacaagc cagaaattat tattccgtta gataaggcta aacgatcaag agcgatgcaa
    16141 ttattgtcta tcgcaatgga caaactagga gtttctccta aaacttatgg aaatgcgaca
    16201 actgtttcca gtgattacga aatcctagaa gccattgaaa aacaggcggc aacaacgaat
    16261 cagctgttat cgttattgct ggcatttttc aaaggaaata gccgaactga tagagattta
    16321 gctttagata ttcagaagat cttggttagg aggatgtaga tgcgaactgt attactgaga
    16381 aatagaataa acgaagaaat tgatttgtcc acagaagact attttgctac tgggttgagt
    16441 aatatggggt ttgaagtaaa aaaagaacat gtgggacagt gggggaattt cagagaaagc
    16501 agtgagagcg ttgaaatatc tgaatttcag tcatctgtaa tcatttctgt gcatgggttt
    16561 cgagaaaaag aactgtacaa ttcacttgtg cagttcctat cggaaggtcc gtttgaactg
    16621 gaatttgctt ttgacggcga aacaatggta agaagatgca gtctaaaatc tttaagtaag
    16681 actgaaatcg atccgaaaac atcgttacta acagacactt tagaactata tttcacatca
    16741 aactggtatt ccgtaaaacg cgaaaaatta attcagcgac cgaatgtagt gaaaacacgt
    16801 ggtaaggttt ttccgtataa aagatcttat atctacactc aaaacttgtg ggagaaaaaa
    16861 ggtgtattca aattcaataa taattctgta tacctaacga atagcaagga acggatgtcc
    16921 ccgctaaaaa ttcgtgtgat tgggaagtgt tcaaatccgt attgggaagt aatccaaaac
    16981 tcacaaatca tcgcaacgga tggatacttc atagatatga ctgaaacaca gactctagaa
    17041 gtatcaagcc tttttgaaga tacaacggcg attttaaaag atattgcagg ggtagaatct
    17101 tctgtctacc aacaacagga ttatacaaaa actaattttg ttcaggcccc aactggagaa
    17161 ttcagcattg tgtttcatgt tgggggggca gacgtagaga ttgagttata cgaggagcgt
    17221 gatctgtttt gattttagca gtcacgcttt ttcatcgtga tttaatgctt tataacgaac
    17281 atttattctc taacagtttt gaatttggtg tggacgagat caacgaagag gctagtagct
    17341 ttacgatgga taagtacgtt cctgtaaaaa caggcgattt tctactagca aaatatattc
    17401 ctagtggaaa atttgcatat tttggtgtaa ttacgtcgca ggaagacgaa aaaatcagtt
    17461 gtaaaagctt acttagttta gctgatagtg agataccgac tgcacgtgtg tcaggagata
    17521 actacgaaga gcatatccgg cggttgattg aatactattt gctgaatgat ccaacgaaac
    17581 aactaaaaga tattttagac gtcaaagcag aaagtgcgac ctctcattcg tatcaagcta
    17641 ccgatacgaa taaacacaag ttaagtgcgt atattctcaa tggtttcaaa aaatacaacg
    17701 taaaatggta tttcaaggga attcaaaaca gaaaaattta tacaggcata cgtgctgtaa
    17761 atgaatcaat ttacattaaa gacaattctt ctgaatttag cgattgggat gtgtttgttc
    17821 aggcgccggg cgctggaaac gagaacaagc tattaatcgt tgataaagca atgaaagata
    17881 tagagaaacc gataatactg tcaacatggt atttggacga agaaaacaat ttgacacaag
    17941 atggatcgaa agaaaatatc acgaaaccaa ctgtcaattt ggttaatatc tacgatcaaa
    18001 ccgcagaaga taaagcatcc tacgaagatg tggcaaaatc agagttgaaa ggcaatacgt
    18061 attcgcatga aatcaaagtg aacgttgtaa gaaatgcaaa aaatttgaat gtcgaaacga
    18121 ttgaaacagg gatgtttgcc acgatttctt ataaaggaaa gatatacaag tcggttttga
    18181 cagcttggcg aatatcaagt gataaggagt ttgtggaatt gactttcgga aacataagaa
    18241 gtcgttttat ggattatttt gaagataatg ggggataaaa aatggttagc aatgtggatg
    18301 gatatcaatt tgaaaacgtg aaagtaagcg cagaaaatga tgctagactt tatcacgttt
    18361 tatataatcg gaaaaatcag gttattgatg gttacgatca gtctatgaat ttatcttcaa
    18421 gcggattaac agtaaaagtt gctgcaggag cagcgattat tcaaggtcgt atggtcgttg
    18481 ttcgacaaga agaaagtata acagttccag caaactcaag tggttatata gcattaacag
    18541 tggatttgac acaagaagtt atacctggat ctattcttcc agaatcggaa gaatatgaat
    18601 ggactaataa tcaagtcaag ctagaattta taacaaaagt tataaaaggt aatttaaaca
    18661 atggtgataa ggtctataat ttaccactat gctcagttaa ttctactgga tcaactgttt
    18721 caatctcaaa gatatcggat agttacgagc tgactctctc taaaggagaa attttgtgga
    18781 gggggactgc gttaatgcat gatactcaaa ctgtccaacc ttcaaaaaaa atttggcaga
    18841 cagtttcagg ttttttgtta gtatggctcc catacgaaaa cgggcaagca attgaggata
    18901 gatatgtaac tacgcctttt tataaggagc gtgtaacttt tactaatgtt ttaggagaaa
    18961 ttgtttcagg atttgatgac tatcacaaaa agtggtttag taaacgaata aactataatt
    19021 cgaatactaa tatatttaca ggtgcagcaa gcaatgcaag tggagataac gcaaatatgg
    19081 tgcttaggta tatagtttct ttttagttag gaggtatgga agagtggcaa atttagaaat
    19141 taaattatct gcaaataaga gacagcctta tctacgtcac cgtgttgttg gtagagttgg
    19201 tgatgggggg ctcacaacaa tcaatgtaca acttcttgag gaagatgaaa ttacaccttt
    19261 tgtaattaat ctaaacggta ctttgaaatt tgtgggcgaa gtttcaaacg gtaactatac
    19321 cgagggagaa ccagaaataa tcgattcgac taatgggtta attagttaca cgttcactaa
    19381 gtcaaatttc agcacgagtc atcaattcaa acaagcatat tttgaatatg tagatcctaa
    19441 cggcaaaaaa gtaacttttc agaacttcat catagacgtg ttagaacgag tggatattaa
    19501 ttcggagcaa gcgaaatact atatttcttc attggaaaaa ttacagagtg aaatgcaaac
    19561 cactttcaat cagttcatta gtgataaaca ggtccaatac gatcaaatct actcgaaata
    19621 taacgaatta gtaagattga taaatgaatc agataagcaa gtaaatgatc gtattgatca
    19681 aaccaatcag caaatcggcg atctcggcaa gctgaaaaag atgtacagta acagcatcga
    19741 ctttgggggc tatgattatt cggggaatcc gaatttgtta agtaagctat catacgactt
    19801 aattgaaaat caaaatactt cagctggaac actttctaag ggtgaaaact cgtttaaata
    19861 taataagata tcagctgaag tggaaggtgg agtagagtta tattataaac gaagaggtat
    19921 agctaactgg ttaccctcta ataaaacgct tgtaatgact gttaagctta gagctggagc
    19981 ggactatagt ccagttgacg gaaaacttat actgattaga tataggtatg ttgacagtgg
    20041 aactggcaag attgttttag acttacctat taacagtaat tcgataactc aggaatggaa
    20101 agagtttagt attactggaa ctactccaac atttagccca caagcatacc atccttggat
    20161 acaatttagg gctcaagatg gggtacttgg ggaaatagaa atgagctatg acatcaaaat
    20221 cgaagaaggc tctacagcta caccatatca gcctaactta cttgatgact cttactggct
    20281 aggtaaaaca ccgttgggtg agaatttgat aacgaatgcc aagttcccaa ttataacacg
    20341 aagcaatcct atttctggtt ttgatatttc agaagaatta ataataggtg aaacttatac
    20401 ggtatcttta aaagggacta agccagctac taaagaattc cacttatact acggagaagc
    20461 aaattaccag cagttgcagt atcaggcaac tttacttcct gtggaaggat taacagatgt
    20521 gtggagtgcc acttttacag ccagaaatac tagtgaaacg acaaatttac ttagagtagc
    20581 gttatggcaa aaacctaatt ctgcaacata tgatactgtt caaattgact ggctcaagat
    20641 tgaaaaaggc gacacacgca caccaaatat cagtcagttt aaatactttg gtgaaggctt
    20701 gaaagacagt aacaatccga acgactacag ctgggatgtc acaccagagt atactgaaaa
    20761 aagcttgaat aatacggtta gtctgactga accgcaatcc atcgaaggat tgaaaaattt
    20821 cgaagatggt attcaatcaa aaggaaaatc tgtattgaca tcagacgaca acaaatatga
    20881 agtcgtaacc ttaacagtta caaacgggaa taccggatca gcaaagcttt atcgtgaagg
    20941 aaaaaccgtc actatttatt tttttgcgtt gaatgggaaa agcagtggtg gaaatgattc
    21001 aacgatacta acaattccag aaggctatcg gccaccaatt agttttgagc aactggttgg
    21061 ctcgatagac cgttctactt tgaacagtgc tcagttatct attggtgcag atggagccat
    21121 taaatggcga agaaactcaa gttatggatc ggattatacc tttgcaatta cttacacgat
    21181 ttagaaaggc gtgaatcgat atgaaggcag catatagacc aattgaacct tacggattcg
    21241 agcaaatcat tgtgaatgat gaagaacatt taccggaaga atgcacagaa gtcgaaccac
    21301 cgattccaaa ttggaaaccg aaattcaatt actgggaggg aaataaatga aaaacatttg
    21361 gaaatatggc cgtactggcg gagagtatgc aggaaaagta ttggacgaca tgcttgtatc
    21421 cgttccttac acagatcagc caccgctcga aggggttcgt tcagatggcg aaccgctaac
    21481 gattgctgat cagatgtttg atcctaaatt gaaccaatgg attattttag cgaacgcgtt
    21541 agatcacaac gatttaaaca atctcaaagc gatgtacgag gctcttgaac atgaaaacgg
    21601 caacctaaaa cagctaaatg ccaaactcat gctaagcgat gtagcgatta aacaggaaaa
    21661 tactgcattg aaagaaaaag ctgacagttt agcacaaatc aattcaaaaa tgatgcttgc
    21721 ttcgttacaa aatagcaaag acatttcaga aattaaagag caactaaatc cagcttcaaa
    21781 gggaggtgag tagtatgttt agttttagcg atgtgaaaat gatgtatgat tggggctgtt
    21841 ttactgacga tcaagttcgt ctattcgttc cactatgtat cacagatgaa gaagcagata
    21901 gaattattag caaagaagag agcgcatctt aagtgatgcg tttttttgtt ggaaagttgg
    21961 tggaacatga aagaagaagc gctccaagac gttgtggaga gattagtaag aattgaaaca
    22021 aaattagaca actacgaatc acttagagaa aaagctgata gtgcaaaaga tttggcagac
    22081 aaagcctact cagtagcgct aaacaacgca gaagatatca aagaaatgaa agccaataat
    22141 aaatggtcgt ggggttacat gatcggttta ggcattacga tcattggcta tttcttgact
    22201 aaattgtaaa ggagttaaga agaaatgatt ttacccgata agtattatca agtcattaaa
    22261 tggacggttt taacagtatt accagctgca tctgttttag tagccacgtt aggaaaagcg
    22321 tatggatgga atggaacaga tatgacagta ctcactatca atgcagtagc aacattttta
    22381 ggcgttatca ctggtgtgtc tgcatataac ttaaaaaaat aggaggaaaa aaatgaagaa
    22441 gaaaatcatt ttatcattga gcctaataat ggctcttttt ttattgccaa ttaacgggtt
    22501 cgcctatacg atcaacaatg aatttaattt aggtgtaaat gaaggtagct c
//
LOCUS       DACDIE010000044        21682 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.044, whole genome shotgun sequence.
ACCESSION   DACDIE010000044 DACDIE010000000
VERSION     DACDIE010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21682
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.044"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..374)
                     /locus_tag="IV250_001859"
     CDS             complement(<1..374)
                     /locus_tag="IV250_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769139.1"
                     /translation="MADIVQLKEDGVAKYLKTHVKAIDGKEALVQTDGDQAIAGHKNF
                     SGSVTINNKRVLTTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGN
                     DSVILTVLEGYRTPISFEQLVGS"
     gene            complement(374..823)
                     /locus_tag="IV250_001860"
     CDS             complement(374..823)
                     /locus_tag="IV250_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769140.1"
                     /translation="MTDIVKIKQSNVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKVKLDGLANITFEKVGTV"
     gene            complement(827..1447)
                     /locus_tag="IV250_001861"
     CDS             complement(827..1447)
                     /locus_tag="IV250_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769141.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
     gene            complement(1447..2355)
                     /locus_tag="IV250_001862"
     CDS             complement(1447..2355)
                     /locus_tag="IV250_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HAP8769142.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETRIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQGVR
                     HESISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFSDNHEVEKIETVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            complement(2368..5127)
                     /locus_tag="IV250_001863"
     CDS             complement(2368..5127)
                     /locus_tag="IV250_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAP8769143.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGEMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAMTSKGEIVADFI
                     KVGIINAEVLESSINDLGDVLKIVKGTLQIWNDNKKIMELTKKGMEFWNTKESIGTIG
                     TTDSAGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGDL
                     DVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWRIYDILCNNGFTKQ
                     SACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSYPLVGPATWDGKVYVQNLFN
                     AAGIKEPITSLDAQVRLLIWTFTNGQWMGVVQPTTVDGFKACTDPRQAAYAFERNYER
                     PAATHPERQDYAVNWYNKFKDLKPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYSG
                     YLGGCGMGAGTKYGLTHVIGNTSAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIINW
                     ARGGQVGSWFADGTYGHTGVIRGLANGRMQTYEQNTELGMICGKLDRQYYSASAISSI
                     VIPPK"
     gene            complement(5124..5828)
                     /locus_tag="IV250_001864"
     CDS             complement(5124..5828)
                     /locus_tag="IV250_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP8769144.1"
                     /translation="MKPDEPNFIWKNRNAVIDMDCIIEGELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLDAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            complement(5840..8149)
                     /locus_tag="IV250_001865"
     CDS             complement(5840..8149)
                     /locus_tag="IV250_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP8769145.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAVESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGESVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGGVKSF
                     IGVWQGLAGLFGISGGWFALAVIAIGALVTGLIWAYNNVKWFHDGMNSFFKGVSDVAV
                     EIFNFLGGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRRGIGGKVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANQQAIYDLGGVPI"
     gene            complement(8344..8808)
                     /locus_tag="IV250_001866"
     CDS             complement(8344..8808)
                     /locus_tag="IV250_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769146.1"
                     /translation="MARLSDYGINVEDLKNSATVTIQGVEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLYKKEGKISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKTQAKRKVNKKNRKR"
     gene            complement(8808..9434)
                     /locus_tag="IV250_001867"
     CDS             complement(8808..9434)
                     /locus_tag="IV250_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP8769147.1"
                     /translation="MGKQDTLYFEGLDDILIAMMATPDTVGTAPTYSEVVRLPIGTKI
                     GVKGNGTALEKWASSKMFRRVSRETKHEIALDHVGIPIEVMDEIKGLVARSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANFVSI
                     GLKYNNVIYASFDSNREEATMDLFEKFIAQPVYDEEQWKTLAKVGGAG"
     gene            complement(9441..9806)
                     /locus_tag="IV250_001868"
     CDS             complement(9441..9806)
                     /locus_tag="IV250_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HAP8769148.1"
                     /translation="MPGNKNGFSEMADYLGNLSRVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMSEQIKVVVDDDRVKVQFEETAFYWRFVENGATNRRAQHFASGTYEQ
                     NKEKIEEIMTKKILDLWEG"
     gene            complement(9796..10134)
                     /locus_tag="IV250_001869"
     CDS             complement(9796..10134)
                     /locus_tag="IV250_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769149.1"
                     /translation="MSISFDELKTALKSTKLPVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAR"
     gene            complement(10124..10450)
                     /locus_tag="IV250_001870"
     CDS             complement(10124..10450)
                     /locus_tag="IV250_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAP8769150.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            complement(10431..10709)
                     /locus_tag="IV250_001871"
     CDS             complement(10431..10709)
                     /locus_tag="IV250_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAP8769151.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETD"
     gene            complement(10711..12087)
                     /locus_tag="IV250_001872"
     CDS             complement(10711..12087)
                     /locus_tag="IV250_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP8769152.1"
                     /translation="MDTELLKKMKARREQRLTELREKVESGELREADLEAITKEIDAV
                     VDELNGIKSELSEADRSDEGSDADEGTGGSDGSTGSDENRSGEGDEGDEGEGGDNDDS
                     ENRSGMISQQQRDGLLGSIKNGLEARAKMTNKQKDQQLRKAFANFVVGNISEAEARAL
                     GIEAGNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKK
                     ERSTDIAETAIQFDEILLDPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEI
                     NYMFNGDDTGNENPGALAKKAVAFEKPLDLTAAGAGQKLYDALIEFKNTPVTEVMKKG
                     RFIINRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTP
                     VLYFGDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRY
                     EITKPVGG"
     gene            complement(12100..12675)
                     /locus_tag="IV250_001873"
     CDS             complement(12100..12675)
                     /locus_tag="IV250_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HAP8769153.1"
                     /translation="MEMKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSPTLLWD
                     DLSEVIAPGAFARTISNSDVRCLFNHDWSNVLGRTKSGTLRLSEDDHGLKFEVDLPDT
                     TVARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTISEVELYEVSIVPLPAYED
                     TEAALRSRDELEKNVEERKKLIKKINQALEA"
     gene            complement(12641..13828)
                     /locus_tag="IV250_001874"
     CDS             complement(12641..13828)
                     /locus_tag="IV250_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP8769154.1"
                     /translation="MEKRGYIEDMFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALSSPVVIGPDGNEVKDHHLLKILKNPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNDSDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQNLKGGDGNEEEGN
                     SHD"
     gene            complement(13892..15619)
                     /locus_tag="IV250_001875"
     CDS             complement(13892..15619)
                     /locus_tag="IV250_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP8769155.1"
                     /translation="MIEPGVNYADLFAKEVRKKPGKYPKTVRLAVDRWYRWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFILEPWEKFIYSWMYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAATILN
                     NPILLEGTKIFKGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGNLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MDLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            complement(15616..16068)
                     /locus_tag="IV250_001876"
     CDS             complement(15616..16068)
                     /locus_tag="IV250_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HAP8769156.1"
                     /translation="MTTKAQRKAIVDEKVSAEKARILKIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWSDKKMKALDLLGL
                     TNKAKTGRQITGGSTARKDEEITRPEEKPVDELAAHRNKWRKKAGTEK"
     gene            complement(16179..16559)
                     /locus_tag="IV250_001877"
     CDS             complement(16179..16559)
                     /locus_tag="IV250_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP8769157.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            complement(16556..16957)
                     /locus_tag="IV250_001878"
     CDS             complement(16556..16957)
                     /locus_tag="IV250_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769158.1"
                     /translation="MAMIKIRTSITGTEYWDSEKKKTVVVPKGQEPKFDTSEQTDMTV
                     FAKAIVGDGVLFKSGEEIHLSGEELDSDGNTAADFDGDEATNDQSTEDTDELDNMTAK
                     ELRAYAKKHGIDIPGAVRAKGDIIHLIREAE"
     gene            complement(16938..17324)
                     /locus_tag="IV250_001879"
     CDS             complement(16938..17324)
                     /locus_tag="IV250_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769159.1"
                     /translation="MNDFHEAVLSIEVEPSLATAYKKAIEDDNSRHWIKNEIKDADGN
                     IVISDIKPSWNGNHCSVSIDNSLTIEKYSGAIVDRTERKIQLTIALLSRTLPNLQEQV
                     EWYERMGAKVISTNYKGENQNGNDQN"
     gene            17807..17986
                     /locus_tag="IV250_001880"
     CDS             17807..17986
                     /locus_tag="IV250_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HAP8769160.1"
                     /translation="MYFVIREATNGQYYFVIKSNNNEVVATSETYLTKYSAEQTINSI
                     KNGITKDSQVIDMTK"
     gene            complement(17979..18392)
                     /locus_tag="IV250_001881"
     CDS             complement(17979..18392)
                     /locus_tag="IV250_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769161.1"
                     /translation="MDKEIKATVKLDLTELKELLNKASDQVEQIKYQGMDRKRLIDII
                     EQQRLELIQKEAVCKEYKKHLEQVIEYHSVEKYRSVVQKNRATSPDPRQDVSISIKTP
                     VFNSVKSEKLESISTALVEKAAEMNSLANEVSRLI"
     gene            complement(18416..18652)
                     /locus_tag="IV250_001882"
     CDS             complement(18416..18652)
                     /locus_tag="IV250_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769162.1"
                     /translation="MYWRVYKMKIASEKIDQLTILVNQRIEDIIKEFEGEKIKNIKLT
                     LMDHKFELNSTKRKIVELIEEEIALQINEQQLTN"
     gene            complement(18941..19354)
                     /locus_tag="IV250_001883"
     CDS             complement(18941..19354)
                     /locus_tag="IV250_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAP8769163.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIETERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGKIIAYK"
     gene            complement(19431..19727)
                     /locus_tag="IV250_001884"
     CDS             complement(19431..19727)
                     /locus_tag="IV250_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769164.1"
                     /translation="MKLKDGFYSSSHGIGGLMLDMPTKNPKTRKKTKVKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMECDKRLAKSKENLAVARLVDMEVIKSK"
     gene            complement(19751..20056)
                     /locus_tag="IV250_001885"
     CDS             complement(19751..20056)
                     /locus_tag="IV250_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769165.1"
                     /translation="MNVQVYLKRDLARFPRSWDEYSCPFEAEEESYLMSANMIEITRD
                     DADEFDKKRSWYSVSNPMYTAVIEDYHISDRFILVDLERPKKRIKQYRRCGFSTKKW"
     gene            complement(20053..20376)
                     /locus_tag="IV250_001886"
     CDS             complement(20053..20376)
                     /locus_tag="IV250_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769166.1"
                     /translation="MSDYLTAEQYEYARKTGDWESGKVLTHGQALNNNQKIVLEYLKS
                     KITESSVNPINAIVILGHLWEFRRFNTEEECLVLEAYWQLNSKQQVEVLQAFSAWVLE
                     EEEAE"
     gene            complement(20373..20651)
                     /locus_tag="IV250_001887"
     CDS             complement(20373..20651)
                     /locus_tag="IV250_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769167.1"
                     /translation="MKYRKKPVIIEAVQFNRSKAEKDVAKYYPMVTDMARLTTAKGTE
                     ERENRFFISTLEGNMTVKDGDYIIQGVEGEFYPCKPDIFEKTYEAVEK"
     gene            complement(20648..21232)
                     /locus_tag="IV250_001888"
     CDS             complement(20648..21232)
                     /locus_tag="IV250_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAP8769168.1"
                     /translation="MNKQELIDKLSSLVGSIEDFKGISTFHAGKYAGLEHALELIQQL
                     NESQKPVIPQLVAGWIEKSTDNLTKAEKIAYLIKSKDGDSYYFCDWFVRDGILTQEQG
                     EELLAWATRQSYETLLSLYNGYEVEKEPLYEVIIGDLYLIKKFNNRNDFCFDTSRSLC
                     TWEKSAYQLTEAEIKEIDERYWPFAVPVEEVAER"
     gene            complement(21229..21642)
                     /locus_tag="IV250_001889"
     CDS             complement(21229..21642)
                     /locus_tag="IV250_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002393044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769169.1"
                     /translation="MIPKFRAWVKPDILSNHLNGVIAEALPNFIYGNCLVLREDLEED
                     KQFEEIIDFLDIELMQSTGMKDKNGVDIFEGDIVKTLDADLKVTFSTIKFEEGAFCVD
                     YKDLGTEFDFLYFVDSPLEVIGNIYENSELLEEQR"
BASE COUNT         6005 a         4827 c         3358 g         7492 t
ORIGIN      
        1 gacccaacca gttgttcaaa actaattggt gtccgatagc cttctagaac agttaaaata
       61 accgaatcat tcccgccact actttttccg tttaaagcca caaaatatat agaaactgtt
      121 tttccttcac gataaagctt tgctgtccct gtattaccgt tagtgacaac cagatttacc
      181 acttcatatc tgttatcatc agtcgtcaaa acacgtttat tattgattgt tacagaacct
      241 gaaaaattct tatgtcctgc aatggcttgg tctccatctg tctgcaccaa tgcttcttta
      301 ccatcaatag cttttacatg cgtttttaag tatttagcaa ctccgtcttc ttttaattga
      361 acaatatcag ccattaaacc gtccccactt tctcaaatgt aatattggct aatccatcaa
      421 gtttcacttt atcggttgct gacattaaac ctgctgtcgt agttgtagca ttacctggat
      481 ttttctgtgc tccagctgca attccatcca acttagtttt atctgtggac gacatcaacc
      541 catttgctgt agttgtagct acagctgtag ttgtggcatt tattccagga tcacctttat
      601 ctccctttgg taaaacaaaa ttaaatctag ctgcagatga tgttcctaca ttcgtaacag
      661 aagcggttga accactagaa acggttccta tggtaattgt tgctgcttgg ccaggatcgc
      721 ctttatctcc cttcaccgtt gttggtttgc cttctatagc attccaatga gtttgtggat
      781 aaacctgtac attgctttgt tttattttta cgatatctgt cattctctat acctccccaa
      841 ttttttcaaa agtaaaattt ggaattcttt cgtttgtgta attttctgct tgatttacag
      901 cttcttggaa tttttgatct acatatgact gattgacacc accagtccca ctaccacctg
      961 tagaactaat tgttccatct tcatcaatag tgatgttttc tccagcagta taatttttca
     1021 gtgaatcgag tttttctttt aactccagcg aaaaattgac atcagattgt ctgactgcag
     1081 agactttccc tgtctcatca atcgtcaata attcaccaac ttttatgcca cccaaacggt
     1141 cgatagtggc aactggcaac acgtagtcac cttctccact gtttaaaatt ttttgataca
     1201 tttctgccgt gataatgccg tcatcttctt cacttgcata gggtaattct gtgagaacat
     1261 tctctaatcc caaatcttct ttagtaatga cgacggctcc aatttttcca ttaacagata
     1321 aaactgttgc ttctcctgct attatttctt ctaacccaag aactgcggaa gcatgtgtga
     1381 ttggaaagaa ttggcgttgt acacctgttt tttcatccgt ttccatcatt cgtttagttt
     1441 tagccattag atcaccccga ctttctcaag agtgaacaca ttttgctttg gatcatcaac
     1501 ggttgcgatg atcaaggctc cttcttcaat tggaaattct actgtttcaa ttttttcgac
     1561 ttcgtgatta tctgaaaaga gatcgtcttg aagaatatca gtcacttcaa tttcaccgta
     1621 ttcaatcgtg aaaagtgctg ctttcatttt ttgatacaaa taatttagat cggataggaa
     1681 acgttcagaa atcgattcat ggcgtacacc ttgaagatct acacgtgcat ccattaattc
     1741 ggctaacatt actccacctg gatcgataga cttcaaaata tccttgattg attcaaacca
     1801 tgacaaataa tctgattctt gtccttctct ccaatcttgg aaactattct cctgttcttc
     1861 tctccagcga tcaaattctt ctttcctttc attcatccag tccgtaaaat cgcctttatt
     1921 ttcattaata aaatctgtca tatcagcaat taaatcttca attgattgcc aataagaacc
     1981 catttctcct tctgtttttg aaacagcatt gatgacaaaa taggagaagt cttgagttgt
     2041 tccaatcaag tcttctcctt taaatataat gaaattggct gtttgtcgat gcaaacactg
     2101 catggaatat ttatcaaaaa tatatttgat tttccctttt ttagcatcca caattcttgt
     2161 ttctaattgg actggatatt ttccgccaac aactgattca aaatatacct tacattctga
     2221 taaatcatat ggaagaccat tttcgacaat tgtagcttcc ataacttcag tgtttttatt
     2281 gccttgtcga acttgaatca tccccacgta attatagggt tctgttgtac ttaatatgac
     2341 attccacttt gccattaaat cacctcccta ttttggtggt atgacaatgg aagaaattgc
     2401 acttgcacta taatactggc gatctaactt tccacaaatc atacctaact ctgtgttttg
     2461 ttcataagtt tgcatacgac cattagctaa gcctcggata acgccagtat gtccataagt
     2521 tccgtctgca aaccatgaac ccacttgtcc acctcttgcc caattaataa ttgcaccaac
     2581 tactaattga tcatatctag ggttttgaat cactttccaa ccaacagcag accaatcata
     2641 ggcaatacca atatcagatg ctgcagaagt atttcctatc acatgagtta aaccatattt
     2701 tgttccagca cccatgccac aaccacccag atatcctgaa tattctgcag acaagccata
     2761 acactgacca ttgccaattc gttgtccaat caaagactct aaatgtttta gtcctgcttc
     2821 tccagtagct cctcctggtt ttaaatcttt aaatttgtta taccagttaa ctgcataatc
     2881 ttgacgttca ggatgtgtcg ctgccggacg ttcatagttt cgttcaaaag catatgctgc
     2941 ttgtcttgga tcagtacagg ccttaaatcc atcaaccgtc gtaggttgta ctacgcccat
     3001 ccattgtcca tttgtaaatg tccaaataag caatcgaact tgtgcatcta agctcgttat
     3061 tggttcttta atacctgcag cattaaataa gttttgaaca taaacttttc catcccatgt
     3121 tgctggacca acaagaggat atgaagaacc atcccactga actaatccgt atgctggtcc
     3181 acctatttga acagtatcag gatcaaaagt tcctcctgtt tcttgttgaa tgttccccaa
     3241 tattccacat gcagattgtt tcgtaaatcc gttattacac aaaatatcgt aaattctcca
     3301 agctctcttt tctgcatctg tttttaattc actaggatac ccaccagtag attctccacc
     3361 tcccgatgga ccaccacctt gtccaggaat tatttctttt cctttaactg taagttttcc
     3421 ttgtacatct aagtctccaa aataaattgc tttaccattt cctaataaaa ctaatccttt
     3481 tcctacttta ggagaaatca aaatatattt gccgtctcca tttgtacgaa taactaaaga
     3541 attatcttca ataggggttg gagtagaagc cccaggaaaa ggattgcctg cagaatcagt
     3601 agtaccaatt gtgccaatgg attctttggt attccagaac tccatccctt ttttggttaa
     3661 ttccatgatt tttttattat cattccaaat ttgtaatgta cctttgacaa tttttagcac
     3721 atcgcccaga tcattaattg agctttcaag gacttctgca ttaataatcc caactttaat
     3781 gaaatcagct acgatttcac ctttcgacgt cattgcgata ccaaacgggc catttactcc
     3841 gttgtctgaa taacctaagc catttaaatt ccaacgccac acacgtttcg cattcgctac
     3901 atttggagta tccatgataa gaatctctga tggagctttt tctggacgaa aaacgacatg
     3961 cccaccagaa ttaccagtga tccatgccgt cgcattcaac acattttgaa ctaacgtttc
     4021 tgttcgattg tcgattttct ttttcaattc ttgtgcttga gtatttactg ccgaagtgta
     4081 cagtgataaa tcattaccta aaacaatatc cttatatttc ccaagcgttg ggaaataagt
     4141 gtattcgacc atgcgctctt ttatctcgat atctagctct ttcgccctaa catgagcgac
     4201 atcaccaaag tgtaaagacg ctagctcttg atacatctca ccgtattcga gtgtgtgttc
     4261 caacgccacc atactgactg tgtgtgtagc ttttggctca tggatacgat cattgtcaaa
     4321 taaggttttg ccccatttga tcaattcatc aactgtttta caatctccgt tttcacgttt
     4381 ggcaatacgc cgattctcgt tggttactcc atcaatttct aaataaccat attcgatagg
     4441 ttctttgtct tcatcataat cgttatcagg aacaccgcca accaaataca aactattgac
     4501 aattgattct tcatcaattt cttcttcgat tgcttctaaa ttgatgccaa aatcaatccg
     4561 aaaaccatta tctgatccaa tctgttttac cagtttcaaa tcatagttat ccatatcaag
     4621 ttccgcgcta gtcacgccag tcaaattttg attaccgttg tttgaaccaa tgattgcttc
     4681 aactggtgcg acttgtcttg ctgtaaattg gtgcgttgtc cctacattcg aaagatagtt
     4741 aaatttttga tcaaatgcca aactgttttt aagattggtc atgatctgag acccattgcc
     4801 gttatccgta aacgacttga caataaagtt cttattggcc ataaaaccaa tatgccgagc
     4861 tgttacagaa acggaggtca aattcttttt gacattataa atttcaaaat attgccacga
     4921 accatccggc accttcgctt ttataaaatt tccttttttt aaataagagc gatactgtcc
     4981 gcttcttgaa tagttaccat aaaaacgata ttggccattc agcacacgat tgatttctgg
     5041 caaatcttcc caatcaacta acgacgctcc gttaacactc aaatcatctg gcattttttt
     5101 gtaagcataa ataaattctt gcgtcacaga tatacactcc tattccaaaa ccgcacttct
     5161 ttaaattttc cggatatttt tacctgattc cattcaggct gcaatactgg ccaatcacca
     5221 cgcgtaaata aattcaaacc ttcttgtatt gctttcccaa gctgagtatc aaccacgatt
     5281 gttgcagcta aagtattaag tattgttagg cttttgtcac cgacagcgat cgtaatatct
     5341 cctccgttcg attctatttc cagataagga tgtgcaattt cgtcaccatg atcaaaaata
     5401 tccataacac tagaatgaaa aattttaggt gcttcaccga tttttctttt gagtggctga
     5461 caacgaaaag taacatcgaa tgtataaaag aagccccact cgttttcgaa tgggacttct
     5521 ttatccatgc tgcagatagc atctagatac ttgtctggat cattatgagt gattaattgg
     5581 cttttaccag taagccatcg tttgacttcc ctcagtttcg aatgtgggat agtgatccct
     5641 tctatttcca aatcaaaagg ttcataatca tcaaacgtct ccgtcaattc tccgcttcgg
     5701 ccctgaatcg tgtaagtttc gtatcgcttg ttcggcataa tatctggaag ctctccctca
     5761 ataatgcaat ccatatcaat tactgcattc cgatttttcc aaataaaatt gggttcatct
     5821 ggtttcataa attgtcgaat caaataggaa caccccccaa atcataaata gcctgttggt
     5881 ttgctttagc aaatttacga ttcatacgat ctaactcaga tggattgttt gcatcgactt
     5941 tgccaatgtg tatatgctgc tcaatactac caccagacac ttttccgcca atacctctgc
     6001 gtttttcttc gtcagataac ggagtaactg tagttttacc attttttgca gtcagtaact
     6061 ctggaccagc ttcaccaaca atcgcttgtc cattaatcag atgaccccct tgcgctaaat
     6121 aaggcagttt agaaatagaa aatgattttc cacctacacc aggaacccat tttggtatct
     6181 ttatattatt caatccacca ataaatccat tgatcaatcc aatcatggca ttaataggag
     6241 ctttccctac agcaacaata ccatcgaaaa tacctccaaa aatatcaact agaccttgcc
     6301 atgcttttga ccaatttcct gtaaatactc cagtaataaa atctagaaaa ccactgaaaa
     6361 ttcgttttcc agcattataa aaattattga aatttgctat aacaccatcg aaaaatccac
     6421 caaaataacc acctaaaaaa ttgaatattt caacagctac atctgaaaca cctttgaaga
     6481 aagaattcat tccatcatga aaccatttaa cgttattata tgcccaaatt agtccagtaa
     6541 ctaatgcgcc aatagctata actgctaatg caaaccagcc tccagatatt ccaaataaac
     6601 cagcaagtcc ttgccaaact cctataaagc ttttaacccc cccaacgatt tttgtaacag
     6661 atcccataag tgaaccaaga acgattagta ctggtccaac tgctgccgct ataccagcaa
     6721 ttgttatgat ccaattcttt gtatcttgat ctagagaccc ccaccattta gagaattcct
     6781 gtaataagga agttgctttt tcaaaaaaag gcaaaaggct tacttggact gattctccta
     6841 cgtcagccat tgctaatttt gcattgttca tagcttgatc ggcttgatca attgggtcga
     6901 gagtagcatc gaatgtgtct cctacagctc ccccactttc tacagcagtt tttgctaaat
     6961 cttccaggtt caaagtacct ctacgaattg catcggccat tctaggaccg cctttagtcc
     7021 cgaatacttc tgctgctgca ttgatggctt ctgtttcaga acctgcattt tttaccttgt
     7081 cttgtagttc ttctagtcct tgacttaaag attttccgtc tttcgcataa gccactgttg
     7141 ctttagataa actacttaaa gctgcgcttg aatcaacacc agattgctca aattgtccaa
     7201 gtaaagtgac cccttcacca aagctcaacc ctaattgttt aatttgtggt gcgccatcaa
     7261 ttgctttttg catcaagtcg tctacagatt gaccagtatt ctgcgatgtt ttcgttgtta
     7321 catcaaggac agaattcaaa tcatcatatt ctagtccata agcatcaatg gcttgtcgag
     7381 cagatattgc tgattgcgaa acatctgtat cattgatttc agcgtactgt aggagataat
     7441 tggttgaatc ttccagtttt ttatccatga atccaaattg tgtatttacc tcaccaatag
     7501 cttccccaac cgtttgtaaa ggtaaatgag tatttgaacc aacgtttttg aaagactgtg
     7561 aaagtctatc agcttgatca cctgttgctc cggtttttgt aatgatggta tcaagtgcct
     7621 cgtcaacttc accaaatgct gcaagtcctg ctgctcctgc cgctacaata ggcgcggtta
     7681 ctccaacaga cattttttca ccaacacctt ttactttctc acctgtttct tcgattttct
     7741 ggagtttttt tgctgtatca acagaaacgt caccttgttc tttaagtgca tcgttcgtct
     7801 gttctaatgc tgtacgaagt ttattttctc cggtttcaga attgagtaat tgtttataaa
     7861 gtttttctga ttcagcagaa tattcgcctg tctctttgac agatttttca tattcttctc
     7921 tcaacatttg agatcgttgt tcagctaacc ccagttgttt ttctaacttt tgtttagccg
     7981 ctcgcaattt ttctgtttga gttgcatcct tgtccatcgc agacacttgg tttttgtact
     8041 cggcagcggc taagttcatt tctttgttga tttctttgat tgttttcgaa taactgactt
     8101 cgccattcgt tttaaaatta agaacgacat cagattcttt accagccatt tatcttagcg
     8161 ctcctttcta ccaccaaggc gatttatcca tagttacaga ttgaggtggt tcaaaatcgg
     8221 tgttttgttg taaccactgt aaataagatt tgagccacaa gttaggtgta gacttcaaaa
     8281 agaaactctc actccaattc aatagagtga gagcaacgta aatataaaaa ctccaaggag
     8341 ttcctatctc ttccgattct ttttgtttac ctttcttttt gcttgcgttt tttgaaagtc
     8401 ttgtggcttc ttggattttt ttaggtcttc cacctgaaat gtctggctag caaaaatttc
     8461 catacaggta ctataagcag acaacacctc tccactcatt cctaagaatt taaaaatagt
     8521 ttctggatct tcctctaaac caccagtgcg caacattgca taaatcaagg cacgcatgat
     8581 ttttaagtca ctaggcgata agttagcaga agaaattttt ccttcttttt tgtatagcat
     8641 ggcattcata tcagattcga attgcgaata atctccacca tatacatctg ctataaattc
     8701 cattgtttgc atagtaaatg agatagggaa ttctacacct tgaatagtga cagtagcaga
     8761 attttttaag tcttcaacgt taattccata atcagataac cgagccatta gcctgcacct
     8821 ccaactttag ccaatgtttt ccactgttct tcatcgtata caggttgagc aatgaatttt
     8881 tcaaatagat ccattgtcgc ttcttcgcga ttcgaatcaa aacttgcata tattacatta
     8941 ttgtatttca gtccaataga aacaaaattt gcagttacat cgtcgatttt tgtctcttct
     9001 tctgcagtgg catactcttc atcaattaca ttagatagtt gtgtgttagg ataccatacc
     9061 gcttttttac cgccattctc aatatttccg atgaatccaa aggcaaaata cggaaattct
     9121 cgtgcggtat ttttgctaaa cgttactccg cttctagcaa ctaatccttt tatttcatcc
     9181 attacttcaa tcggaatacc cacatgatcc aacgcaattt catgttttgt ttctcgcgat
     9241 acacgtcgga acattttact tgatgcccat ttctctaacg ctgtaccatt acctttgacg
     9301 ccgatttttg taccgatagg caaacgaaca acttcactat aagttggcgc agtcccaacg
     9361 gtatcaggcg ttgccatcat cgcaattaaa atgtcgtcga ggccttcaaa atataaagta
     9421 tcttgttttc ccaattaaaa tcacccttcc cataaatcaa gtattttctt tgtcataatc
     9481 tcttctattt tttctttgtt ttgttcatat gtcccacttg caaagtgttg ggctcttcgg
     9541 ttagttgctc cattttcaac aaatcgccaa taaaaagcag tttcttcaaa ctggaccttc
     9601 actcgatcat catcgaccac aacttttatt tgttcgctca tatgtttttt cttaagcagg
     9661 gacttaggaa tattgggaag caactgcttt agataaaagt tagctgcttc ttctaaggat
     9721 tccaaggata attttttggg atcaactctc gaaagattac ccaaataatc agccatctcc
     9781 gaaaatccat tcttattacc tggcattttc cacacacctc acatatgtgt aatagttggt
     9841 cacggtatcg tcattctcat caccttgtat acctgtaaag tctgaatatg gaatgccagc
     9901 attttgcagc gcttgttcaa taacaactaa atcctgttct gttccaagtg taaaaaaaga
     9961 gacttggtaa taaggtaatt ttttatagac tttaccggat gccatttttt tgctgttact
    10021 cacatttgag tacacaatgt atggatacat cgtccctaat ctggctttgt ctctgaacac
    10081 tggtaacttt gttgatttca gcgctgtttt caattcatca aagctaatcg acataagcta
    10141 aactcaactc catttctctt gcatcgggat tcgtataaat gcgagtaatg ttatacgtta
    10201 cagaatcaat tttgagcgca cttaatttct ctgtgatgga tttatcccat ctgactttaa
    10261 ttcgtctgac aacgtctgtc ttggcttgct gtgataaata tttttcttga gaagtcacac
    10321 cgagttcttc atagaatatt agacgcttaa attcgtaaat tgtagttgga cgatcgtttc
    10381 cgtctgttcc tgttttgatg tctagcaatt cggctttcca tctgagatta ttagtctgtc
    10441 tcttcggcat tttgaatcgc tccttgcacg ataaatggcg tcatggcatt catagctttg
    10501 tcgagttcat cctctgaaac tctatattca taggcaatgc cggcaaccat caaaataaga
    10561 tattcttgtt ggccaccagt tgctgttttg acataatctt ttgccatatt taaataaaaa
    10621 gagagcaaag aatcatccat gccctcttca aaatgaatat gtgatttgaa tttttcctct
    10681 aaagacaatt ctttagtttg ctcttccatc ttaaccacca actggttttg taatttcgta
    10741 gcgatatact gccggttcaa atggagaata aaccaattga ccatctagca agttgtaaat
    10801 ttggaatcca atttgatttt taccagagaa tttttcaaca agtttttgaa tttccaaggc
    10861 accaataact tcttgaattt taaatgcaga aaaatcgcca aaatataaaa ctggtgtgtc
    10921 tggttcaccc tttttatctg ctgcatctgt ccaatccaca ggatagccaa ctaattggta
    10981 accaattcca ccttctgctt gtgtgaatgg ccgcaacaaa ggaaatccat catctgtttt
    11041 cattttttca atggcagtca aagcagctcg attaataata aagcgtccct ttttcatcac
    11101 ttctgtcact ggtgtatttt taaattcgat taatgcatca tataattttt gcccagcacc
    11161 tgcagcagtt agatctaaag gtttttcaaa tgctacagcc tttttggcta atgcaccagg
    11221 attttcattt ccagtgtcat caccattgaa catataattg atttctttac gcacataagc
    11281 ttttttcaat tcttccacaa caatatcttc aactggaaca ccagacattt ttagtaattt
    11341 tttagttact gttgccaaag catcgaattc ggcaggatca agcaaaattt catcaaactg
    11401 aatagctgtt tcagcaatat cagttgaacg ctctttcttg tttacattcg catctgcttt
    11461 cttcacaaga attggatatt tgacatctcc tgatgttcgc accactgttc cgtatttacg
    11521 aagtaaattt tcttcttgag cataagtaat aacttcagat gcaattactt ctgggacagt
    11581 aactgaaccg ttgccagctt caatccctaa agctcgagct tctgcttcag aaatatttcc
    11641 aactacaaaa ttagcaaatg cttttcgtag ttgttgatct ttttgtttat tggtcatttt
    11701 tgcacgtgcc tccaatccgt tcttaattga tccaagtaat ccgtctcgtt gctgttgact
    11761 aatcattcca gaacgatttt ctgaatcatc gttatcccca ccttcccctt catcaccttc
    11821 atcaccttca cctgaacgat tttcatcgct tccagtcgag ccgtctgatc cacctgttcc
    11881 ttcgtcagca tctgaacctt catctgatct atcggcttca cttagttcac ttttgattcc
    11941 gttcaattca tcaacaacgg catcaatttc ttttgtaata gcttctaaat cagcttcgcg
    12001 taattctcct gattcaactt tttcacgtaa ttcagtcaat cgttgttcac gacgtgcctt
    12061 catttttttc aataattctg tatccatgta tttttcctcc tacgcttcta gcgcttgatt
    12121 aatttttttg attaattttt ttctttcttc gacgtttttt tctaattcat cacgacttct
    12181 taatgctgcc tctgtatctt cataagccgg caaaggaaca atagaaacct catataattc
    12241 cacttcgcta attgttcgaa gcataggttc tgaattgtag tcccatgttt cttcagttgg
    12301 tacaaatcca aaactacatt gattaatgtc tccgcgttcc atagatttaa ccaagtctct
    12361 tgctaccgtt gtgtctggca aatcgacttc gaatttcaaa ccatgatcgt cttctgacaa
    12421 tcgaagggtt ccgcttttgg ttcgccctag tacattagac caatcgtggt taaataagca
    12481 acgtacatcg gagttactaa tcgttctagc aaaagcgcct ggtgcaatga cttcactcaa
    12541 atcgtcccat aatagtgttg ggctattgaa tacagcagca tatccactaa tggtcctcgt
    12601 atgagtttct tcatcagaac gcgttgaaag gttggtgatg tcaatcgtgc gaatttcctt
    12661 cttcttcatt tccatcacct cctttcaagt tttgatcatt cgttggtaag gaatcatctg
    12721 ttgcattttt ctggccaatc ctagacaagt catttgaaat atagatagct tgtgtttctg
    12781 gagtgttctg tttaggaaaa ccaagcattt ctgccacatt atctggactt gtaatcccag
    12841 tacgaacaat gttgtagcca atatttgttt ttgttgagta aggaacaaaa tccaaaatgt
    12901 taattttcca ttccactcga tagccagaat taggcataaa aaaaagagcg gtgtaatgtt
    12961 cgctcttgtt cttcaatatt ggtttgattg ctttgttgtg cagatacatc atcgcttttt
    13021 cgatgtctgt tttcatcaac gattgatacg tatcaacatt gattcctaaa aatttaccta
    13081 agtctttttt ataaacacct aaatagttca aaatagccgc gtcatcaaca ggacttttta
    13141 acgtctcgat ggaatatcct tttcccagag gaatcatctt aacagaatga tcactgtcat
    13201 tttgcgttcc ttccagttga tccaatatag ctttgacaat tttcgtttgg gcgctattat
    13261 ttggattgat gtgggcgtcc agtttaagca tgaacgcgag taaaccacct ttagtatatt
    13321 tgtccgtcaa aactttttca gcgctcagaa cgccttccag agtgtttctt gcaaggtcaa
    13381 ttattccagc accttttaat gaatcagttc cgatgttctt aatatgtcga atcatttgac
    13441 caggtatttt ttgtccattc atttcaaatt cttcttgaag tcgttcattg attttagtgg
    13501 ttacaccgta tgccaaatga agctggtccc gatctgttaa tgggaatgtc tcaccattga
    13561 tcagtagggt atttgtttcc aatttggtaa attcgaatcc ggtcaaataa tcattgggat
    13621 tcttcaaaat ttttagcaag tggtggtcct tcacttcatt accgtctgga cctatgacaa
    13681 caggtgagga caacgctacc tggtttgaga tatcctggac caattcataa acatcagaag
    13741 attccatgat agaggaatct gttacatatc tttgaccgta acgcgtatag tggccaaaca
    13801 tatcctcgat gtacccacgc ttttccataa aggaatagac tgcattcgat aaccgatcac
    13861 gtaatttcaa tatttctcac cgcctttcta tttatctata gatggaacta aggtaatcat
    13921 ctaattcatc cgaatcaata tctgtcatct gattcatcgt ttccttatga ccacacaaaa
    13981 acgccacgaa accatcaatc tttttctttg attgacgttt acttggcgct ttttgtccgt
    14041 tgatgttagt gattgctaca acgttcaagg tgcaataaag gaacaatgga ttatcaaatt
    14101 gaattcgttt ctcataaaac aaccgttcga catcatcaaa aggagcgttc aacactttag
    14161 gatattgggc aacctcaacg cattccaatc ctaagttctc caatttctca acaagtttgt
    14221 cgctcatcgc tggatcataa ttcacttgtt gtatatcgta taaatccatg cagtcttcga
    14281 tgtactgcaa gatttgatct tgatcaatca ttttaccatc gcaaaattca acaaaacctt
    14341 gttcagctaa atcgctgtaa ggcacgttat cttctttttc tcgaaactct aaatcttcat
    14401 tgggaataaa ataaagctgc ttcactttaa ggaccgcttt tccatcttca tcccacgttg
    14461 ggaagtttaa agacacacag gtcaaatctc gtgtgcgtga taaatccaaa ccaatgtaac
    14521 aaggttcacc acttaaattt ccaagctctt gggtagtaac caaacacggc tctacttgat
    14581 cctgttcaaa gaaattatcc gcaccgttta caaatacatc caagtgcttc gttagaaact
    14641 cggcttttga atgtgctgaa cgttgtgccg ttttaaacgc tgactccaag gcggaaagat
    14701 caactgatat tccccagtta ggattgcaca tttcccaaac ttttctatcc gtccaatcat
    14761 agtttttatt tggttcatag attagaacaa agttcgaatc attatcatca cgtttcaaca
    14821 cttcttttgc ttcacgatat acgcgcattc caacagacga tgaacctttc ccagcagttg
    14881 aaatattaaa catcaatggt tgtggcaaag agatttgtgc agacttaaaa ttgtcatact
    14941 gttccatttt ctcttgctta tgcagctcat cattcaatac aaaatatgga ttagaaccct
    15001 caatgttatc gatattcttc gtttgaacaa taaatttatt cgaataagcc atatcttcat
    15061 gaatataatc ataagtaata ctagaaacag ttccctttgg tcctttgaat attttagttc
    15121 cttcaagtag gattggattg tttaatattg tagctgcaaa aggtttggca gcatattgag
    15181 cttgagcaaa gtcagaagcg catgcataac aatcgactga caaagcacct tctccataca
    15241 ttgcatagcc taatgaaccg acagcaatca atgttttacc gttcttttta gggatttgga
    15301 catacgcttc acgagtaaca cggacaattt gccctttttc attttcttta acccatccat
    15361 acatccacga gtaaataaat ttttcccaag gttctaaaat gaatggcttt ccaaccatct
    15421 caccttttgt gtgaacaata aacgactcaa cccagtccat catttcattc gcacgatcta
    15481 catcaaacca aatatcttta cgttttttcc accgatacca acgatccact gccaaacgaa
    15541 cagttttagg atatttccca ggtttctttc ttacttcttt tgcaaataaa tcggcataat
    15601 ttacaccagg ttcgatcatt tttcagtacc tgccttctta cgccatttgt ttctgtgcgc
    15661 tgctagttca tctactggct tttcttctgg acgtgtaatt tcttcatctt ttcttgcagt
    15721 cgatccgcca gttatttgtc taccagtttt agccttattc gttagcccca acaaatctag
    15781 agctttcatt ttcttatctg accaagtttc tacttgctgc gccaatggat gcttcgattg
    15841 attagtggca cctgatttat tcgtgaattt ttgcgtctcc ggaaagcctt tttccttcca
    15901 caaaagatat ttgtgttggt aaatttcaaa aatatccaaa tatgattcga ttaatggatc
    15961 aagagtgatg gtgtacaaat cagacaaatt cattattttt aaaatacgag ctttttcagc
    16021 acttactttt tcatcaacaa tcgctttacg ttgcgcttta gtcgtcatac ttgtatacac
    16081 ccccctttgt tttttgaaaa atttgaccta acgatgcgcg tgactccccg ctaccctatc
    16141 tccccacgcg aaaaaatttt gaaaatggat agggggggtt aattaaaata cgaaggaaaa
    16201 acttttttgt cttctgtttc gttttcaaca attggatgac attttgaaca taaaagcatg
    16261 agattgtttg gatcaagctt aagcagttca ttatctttga ttggtacaat gtgatggacg
    16321 tgtgccctct ttccaaagat gaactgacca catcgctgac agtggccacc ttctctttcg
    16381 taaataaatt ggcgcatatc tttccatgct tgcgttcgat agaatggttt gttctcatga
    16441 tgataaacag actttgcttg ctgcttcttc ttgcgtgatc tgcttgatct cttgtgttca
    16501 gtacagtaga taccctttgc tatcttgttc gtgcatccgt caaactgaca gtacttcatt
    16561 ctgcttcacg aataagatgg atgatgtctc cttttgcacg gacagcacca ggaatatcaa
    16621 tgccatgttt cttagcatat gcacgcaatt cttttgcagt catattgtcc agttcatccg
    16681 tatcttcagt ggactgatca ttagtagctt cgtctccatc aaaatcagca gcggtgtttc
    16741 catcgctatc taattcttct cctgataaat gaatttcttc accactcttg aacaagacac
    16801 catcaccaac gattgccttg gcaaatacag tcatatcagt ctgttcactt gtatcaaatt
    16861 taggttcttg acctttcgga actacaacag tctttttctt ttctgaatcc caatactccg
    16921 ttcctgtgat ggatgttcta attttgatca ttgccatttt gattctctcc tttgtaattt
    16981 gtactgatta cttttgcacc cattcgctca taccattcaa cctgctcttg taagtttggc
    17041 agtgttctag aaagcaatgc tatggtcagc tgaattttcc gttctgttcg atcaactatt
    17101 gctccagaat atttttcaat agtcaggcta ttatcaatac tgacactaca gtggttgcca
    17161 ttccagcttg gcttgatatc gctaatcaca atatttccat cggcatcttt tatttcattt
    17221 ttaatccaat gcctactgtt gtcatcttca atcgcttttt tatatgcagt ggccagactt
    17281 ggttctacct cgatgctaag taccgcttca tgaaaatcat tcatagataa aactcctttc
    17341 aaaataaaaa gaccactcaa tgagtgatct aatatgtaat acaggaccca ctatccttcg
    17401 agggaatgtg gattacagaa acatcagcgc gctatagttt ccaacctgta tattcggttt
    17461 tactgacagg cagttatgga tcaccgtaaa ccaaagtcac tggcaatgaa tcgaacattg
    17521 caacaccatt ttccgatatt ttacagactc tccatcataa aaactctcac ctctagaatt
    17581 gtctatcttt aggggattta tttcaagatg cctcttgtcc tttaccctcg acggtctctt
    17641 gcctagctgc ataaactaag ctcaggtgct cattaccact gatttaagtt attagcgtta
    17701 cctttccgcc acagtgacaa atttatattg tgaaaataaa tactaagcgt ataattttaa
    17761 ttatcagcga gtggtccgct gaaataaatt ataggtggtg aaaaatttgt actttgttat
    17821 aagagaagca actaatggcc aatattactt cgttattaag tccaataaca atgaagtagt
    17881 agcaactagc gaaacatact tgactaaata ttccgctgaa caaaccatta attctataaa
    17941 aaacggaatc accaaagact ctcaagttat cgatatgact aaataagacg actaacttca
    18001 tttgctaggg agttcatttc agctgctttt tctaccaaag cagttgagat actttctagc
    18061 ttttctgatt taacagaatt aaatactggc gtcttgatac taatcgatac atcttgacga
    18121 ggatcaggac tcgttgctct atttttttgc accactgatc tgtacttttc tactgaatga
    18181 tactcaatta cttgttctaa atgttttttg tactctttac atacagcttc tttttggatc
    18241 aattctaaac gttgttgctc aataatatca atcaaccgtt ttcgatccat accttgatac
    18301 tttatttgtt cgacttggtc actagccttg ttgagcagtt cttttagttc agtcaaatct
    18361 aatttgacag ttgctttgat ttctttatcc attcgttttc cctccaatac ataaattaat
    18421 tagttaattg ttgctcattg atctggagag ctatttcttc ttctataagc tcaacgatct
    18481 tcctttttgt cgaatttaat tcgaacttat gatccattaa agtaagcttt atgttcttaa
    18541 tcttttcacc ttcgaattct ttgataatat cttcgattct ttggtttact aaaatagtta
    18601 gttgatcaat tttttcggaa gcaatcttca ttttatatac cctccaatac ataaattaat
    18661 agacagcagc agttgaaaga cgacaagaac atgtttagtt ttgtgagtgc tgctgtctaa
    18721 tcaaaataaa cagcaaccga tgaagaattt aggaggagtt gaattcacat ccttttcttc
    18781 ataattagtt gctgtctatc taagaagaag tttaaaacga tgagggagat tgcctccctt
    18841 cgtttatttt gtcgatcctg tttcctaatc tttcgacact accataataa cactggtaaa
    18901 tgtctaaaaa ccgccatcat tccgccaaaa aaccgccaaa ttatttataa gcaattattt
    18961 ttccgtgttt atatgcttct gcaaactcta ttagagcttc cgacttcatc cgttgtatac
    19021 ttctttctga ataacccact tcacggctaa ttctgtagtt tgagaagcta tctggcacac
    19081 agaagctgta gtagagtatc tgacgactaa tcagactaag agccatcaaa gccgctagaa
    19141 tcgcatctct ctccgtttct atatccatca tctgaataat cgcgtcttct gccttattgc
    19201 catgcttcgg tgccttcggc atatccgtta taatcggcga cttaatatct atcaaagagc
    19261 gacctgccat ccgctccaaa cgccgaaagt tcttcagcac atctctcgca ttacatcttg
    19321 tctgtttgaa atctacctct cgtaacaatt gcatcaagtc aaaccgctcc tttatgtgat
    19381 ataataaatg tgttggattt attgaatcag tcggagcgat ccggcttttt ttatttgctc
    19441 ttgattactt ccatgtcaac taatctcgct acagctaaat tctctttgct tttcgctaac
    19501 cgcttgtcac attccatcgt gttttcaatg cgaatgattg ctgagtttga cagtatcttt
    19561 ttaacgtatc ctcggaaggg ataaacgaac tcctctgctt cacagcgaac catgtcacca
    19621 acttttactt ttgttttctt acgtgtttta gggttctttg tcggcatgtc tagcatcaaa
    19681 cctccgatac cgtgactgct agagtagaat ccgtctttta gtttcattac tctacctcct
    19741 gttttaattt tcaccatttc tttgttgaaa acccgcaacg tctatactgt ttaattcgtt
    19801 tcttaggtct ttctagatca accagaataa atctgtcgga tatatgataa tcttctatta
    19861 cagccgtata cattggattc gaaaccgagt accatgatct ttttttatca aactcatccg
    19921 catcatctct tgtaatttct atcatgttag cagacattag atagctctct tcttctgcct
    19981 cgaatgggca ggaatattca tcccagcttc gaggaaacct ggccaaatct ctttttaaat
    20041 aaacttgaac attcattccg cttcctcttc ttccaacacc catgcgctga acgcttgaag
    20101 gacttctact tgttgcttac tattaagttg ccagtacgcc tcaagaacta gacactcttc
    20161 ctctgtatta aaccttctaa attcccacag atgtccaagt ataacaattg cattaatggg
    20221 atttaccgaa gactcggtaa tttttgattt aaggtactcc aacacgattt tctggttgtt
    20281 gttgagtgct tgcccatgag tgagaacttt acccgattcc caatctccag tttttctagc
    20341 atattcatat tgttctgctg ttaagtaatc gctcattttt ccaccgcctc atatgttttt
    20401 tcaaaaatat ctggcttaca tggataaaat tcaccttcca caccttgaat aatatagtct
    20461 ccatctttaa cagtcatatt cccttctaaa gtagaaatga aaaatctgtt ttcacgttct
    20521 tcggttcctt tcgctgtagt taatctagcc atatctgtaa ccatcggata gtactttgca
    20581 acatcctttt ccgcttttga tcgattaaat tgcaccgctt caataattac tggtttcttt
    20641 ctatatttca tctttctgcc acctcttcca ctggcacagc aaatggccag tatctttcat
    20701 caatttcttt gatttccgct tctgttagct gataagcaga tttttcccaa gtacacagcg
    20761 agcgactagt atcaaaacag aaatcatttc tgttattaaa tttcttgata agatataagt
    20821 caccaataat aacttcatac aacggttctt tctcaacctc gtagccgttg tataggctca
    20881 atagtgtttc atatgattgt ctcgttgccc aagcgagtaa ttcttctcct tgttcttgcg
    20941 tcaatatgcc atctcgcaca aaccaatcac agaaataata tgaatcacca tctttggatt
    21001 tgattaaata cgctattttt tcagcttttg ttaaattgtc ggtacttttt tcgatccaac
    21061 ctgccacgag ttgtggaata actggtttct gcgattcatt tagttgttgg attagttcta
    21121 atgcgtgttc taaacccgca tattttccag catgaaaagt acttatacct ttaaagtcct
    21181 ctatgctacc tacaaggctt gatagtttat caatcaattc ctgtttattc atcgctgttc
    21241 ctccaataac tcgctattct cgtatatatt cccgatgact tccaaaggtg aatctacaaa
    21301 gtacaaaaaa tcaaattccg tgccgagatc tttgtaatca acacaaaatg cgccttcttc
    21361 aaactttatc gttgaaaaag ttactttcaa atcagcatcg agagttttga caatatcccc
    21421 ttcaaatata tccacaccat tcttatcttt cattcctgtg gattgcatga gttcaatatc
    21481 aagaaaatca ataatttctt caaactgctt atcttcttct aaatcttccc tcaaaactaa
    21541 acaattgcca taaataaaat taggtaatgc ctcagcaatc accccattaa gatgattgct
    21601 taaaatatca ggttttaccc acgctctaaa cttcggtatc attcgctgtc ctcctcaaat
    21661 actcttctaa tatttcttta ta
//
LOCUS       DACDIE010000045        20836 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.045, whole genome shotgun sequence.
ACCESSION   DACDIE010000045 DACDIE010000000
VERSION     DACDIE010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20836
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.045"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            72..266
                     /locus_tag="IV250_001890"
     CDS             72..266
                     /locus_tag="IV250_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769170.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            535..834
                     /locus_tag="IV250_001891"
     CDS             535..834
                     /locus_tag="IV250_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769171.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(931..1365)
                     /locus_tag="IV250_001892"
     CDS             complement(931..1365)
                     /locus_tag="IV250_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8769172.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1597..2307)
                     /locus_tag="IV250_001893"
     CDS             complement(1597..2307)
                     /locus_tag="IV250_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP8769173.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2297..3157)
                     /locus_tag="IV250_001894"
     CDS             complement(2297..3157)
                     /locus_tag="IV250_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAP8769174.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3162..3800)
                     /locus_tag="IV250_001895"
     CDS             complement(3162..3800)
                     /locus_tag="IV250_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAP8769175.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3815..4114)
                     /locus_tag="IV250_001896"
     CDS             complement(3815..4114)
                     /locus_tag="IV250_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8769176.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4157..5641)
                     /locus_tag="IV250_001897"
     CDS             complement(4157..5641)
                     /locus_tag="IV250_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP8769177.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5661..6122)
                     /locus_tag="IV250_001898"
     CDS             complement(5661..6122)
                     /locus_tag="IV250_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8769178.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6140..7207)
                     /gene="ulaG"
                     /locus_tag="IV250_001899"
     CDS             complement(6140..7207)
                     /gene="ulaG"
                     /locus_tag="IV250_001899"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAP8769179.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7425..8195
                     /locus_tag="IV250_001900"
     CDS             7425..8195
                     /locus_tag="IV250_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP8769180.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8468..9295)
                     /locus_tag="IV250_001901"
     CDS             complement(8468..9295)
                     /locus_tag="IV250_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8769181.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9863..11098
                     /locus_tag="IV250_001902"
     CDS             9863..11098
                     /locus_tag="IV250_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAP8769182.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12097..12357)
                     /locus_tag="IV250_001903"
     CDS             complement(12097..12357)
                     /locus_tag="IV250_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP8769183.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12384..13685)
                     /locus_tag="IV250_001904"
     CDS             complement(12384..13685)
                     /locus_tag="IV250_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8769184.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(13977..14435)
                     /locus_tag="IV250_001905"
     CDS             complement(13977..14435)
                     /locus_tag="IV250_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP8769185.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(14448..15773)
                     /locus_tag="IV250_001906"
     CDS             complement(14448..15773)
                     /locus_tag="IV250_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAP8769186.1"
                     /translation="MKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDEER
                     NMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKIPL
                     KHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVRRK
                     YPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREIMP
                     EIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVVEH
                     TDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLNDDR
                     RRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAVEK
                     LLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(15790..17202)
                     /locus_tag="IV250_001907"
     CDS             complement(15790..17202)
                     /locus_tag="IV250_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8769187.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            complement(17219..17983)
                     /locus_tag="IV250_001908"
     CDS             complement(17219..17983)
                     /locus_tag="IV250_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HAP8769188.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(18087..18962)
                     /locus_tag="IV250_001909"
     CDS             complement(18087..18962)
                     /locus_tag="IV250_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8769189.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(19217..19301)
                     /locus_tag="IV250_001910"
     tRNA            complement(19217..19301)
                     /locus_tag="IV250_001910"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19265..19267),aa:Leu,seq:caa)
     gene            complement(19319..19390)
                     /locus_tag="IV250_001911"
     tRNA            complement(19319..19390)
                     /locus_tag="IV250_001911"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19356..19358),aa:Gln,seq:ttg)
     gene            complement(19400..19472)
                     /locus_tag="IV250_001912"
     tRNA            complement(19400..19472)
                     /locus_tag="IV250_001912"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19437..19439),aa:His,seq:gtg)
     gene            complement(19534..19618)
                     /locus_tag="IV250_001913"
     tRNA            complement(19534..19618)
                     /locus_tag="IV250_001913"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19582..19584),aa:Leu,seq:caa)
     gene            complement(19639..19709)
                     /locus_tag="IV250_001914"
     tRNA            complement(19639..19709)
                     /locus_tag="IV250_001914"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19675..19677),aa:Cys,seq:gca)
     gene            complement(19755..19826)
                     /locus_tag="IV250_001915"
     tRNA            complement(19755..19826)
                     /locus_tag="IV250_001915"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19792..19794),aa:Gln,seq:ttg)
     gene            complement(19838..19910)
                     /locus_tag="IV250_001916"
     tRNA            complement(19838..19910)
                     /locus_tag="IV250_001916"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19875..19877),aa:His,seq:gtg)
     gene            complement(19913..19986)
                     /locus_tag="IV250_001917"
     tRNA            complement(19913..19986)
                     /locus_tag="IV250_001917"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19952..19954),aa:Trp,seq:cca)
     gene            complement(19993..20073)
                     /locus_tag="IV250_001918"
     tRNA            complement(19993..20073)
                     /locus_tag="IV250_001918"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20037..20039),aa:Tyr,seq:gta)
     gene            complement(20075..20150)
                     /locus_tag="IV250_001919"
     tRNA            complement(20075..20150)
                     /locus_tag="IV250_001919"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20115..20117),aa:Phe,seq:gaa)
     gene            complement(20186..20258)
                     /locus_tag="IV250_001920"
     tRNA            complement(20186..20258)
                     /locus_tag="IV250_001920"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20223..20225),aa:Asp,seq:gtc)
     gene            complement(20268..20340)
                     /locus_tag="IV250_001921"
     tRNA            complement(20268..20340)
                     /locus_tag="IV250_001921"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20305..20307),aa:Val,seq:tac)
     gene            complement(20356..20427)
                     /locus_tag="IV250_001922"
     tRNA            complement(20356..20427)
                     /locus_tag="IV250_001922"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20392..20394),aa:Glu,seq:ttc)
     gene            complement(20442..20531)
                     /locus_tag="IV250_001923"
     tRNA            complement(20442..20531)
                     /locus_tag="IV250_001923"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20495..20497),aa:Ser,seq:gga)
     gene            complement(20571..20644)
                     /locus_tag="IV250_001924"
     tRNA            complement(20571..20644)
                     /locus_tag="IV250_001924"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20608..20610),aa:Asn,seq:gtt)
     gene            complement(20654..20769)
                     /gene="rrf"
                     /locus_tag="IV250_001925"
     rRNA            complement(20654..20769)
                     /gene="rrf"
                     /locus_tag="IV250_001925"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         6563 a         4379 c         3321 g         6573 t
ORIGIN      
        1 tagatacata atcattttca cttacatata tttgaatatt aactaaaaac agcaagaaag
       61 aagaggatca aatgtattat gtagaagtaa aaacaaaagg cgtcaaaaac aaacaatatg
      121 tgaaaggtat cagtaacgaa tatcctttac ttggttcatg gaaagaagct gctccttttt
      181 ccaaaccatg tgccataaaa ataaagaacg agctagaaaa agaactgact tgtggtaaag
      241 ctgttgtaga tatcatcgaa aagtaaatcc aatgtataga aaggaagatc acacatgaaa
      301 agcgacgtac aaattgctaa gattatagaa ttagaagaag aagacgcagt gttgacaatt
      361 gaaaaaaaat aaatgtttgt ataccatatg attatttcaa tatgtatcct ggaattggag
      421 atctcgtaga gctatatcaa gatgaatcaa atatacttgt cattcatcat gaaatataga
      481 gacttataaa attgtttatt tttataatgt atcagtatag aaaggaaata aaacatggat
      541 tatactgttg aatttgacag tgaactactt aagcaatttc cacaagaaga acatttcatg
      601 tataaagaag caatttccca caaagtgaca gagtatttgg agcataattt ttttcgtata
      661 aagccagtgc gtactcgttc ggcaaaagga atatacgaga tgaaagtcaa aattaataaa
      721 aaaaattatc gcatagcatt tgctttaaaa aaaaatcatg tgttcatttt ttatattagt
      781 tcgaatttgc aaaaaattgt atttgataaa gaggtgagta aaaaaataaa atagctgagc
      841 attggaggat agattgagtg atactaagtc tatcctcttg tttttctaaa gaactaagct
      901 agaatttttt cttttgtacg ttattatatg ttatttgtca ttaccaaaaa gcaccttttt
      961 tcttctagta tctatgacat aagaaatacc acccatttcc tttacttgaa caaatcctac
     1021 tgattcataa aatttttttg ttttctcagt atcatctgtc aataattcga tttggactgg
     1081 tccataacta gtgatcttct ccaaagcttc attcaaaagc actttgccta ttccttggcg
     1141 ctgatacctc ggatgaatga ggatatcttg aataaatgcg atcgtcgcaa aatcagtgat
     1201 ccaacgaacg attccgataa tcttcccttc cccattacgc tttatcagag attcagattg
     1261 ttcaaatgct ctagctaacc ttgcagtgtc ttttgtgtaa ttcgtccaac caacgctctt
     1321 atataattca gaaatctcat cgatcataag ggaatcaata ttcatttttt tcttcctttc
     1381 ttatacaatt ctccctctaa taatacagtt ctttgactat ttaatctatc acatataact
     1441 ctcctgtaaa gattctctcg attccttaaa attcctcttt ccttttagaa tgcatacttt
     1501 ttgataaata aaaaggttgt gataaatttt cttcacaacc tctgtttctc tctatcaagt
     1561 gttatctttt aattttctac ccttacccta gcatctctat tgcccgtagt atgcatactt
     1621 tccatgtttg cgaaaataat gtttatctag taaatattgc gggatttcac atatattggg
     1681 gttgatcagc tcgttttctt tggccatcag acaaatttca tctaagatca aactgttttc
     1741 tactgctttc ataggtgaat ccccccatgt gaacggtcca tgtccataga caagtactcc
     1801 agggacttcg tttggatcta attttctttc cttaaacgtc tcgacaatca cattccctgt
     1861 atgaacttcg taagcttctc ttacttcttc tttcgttaat tggcgtgtac aaggaacctt
     1921 accataaaaa gcatcagcat gagtcgtgcc ataagctggc aaatcgcgtc ctgcttgcgc
     1981 ccacatgacg gagtgcgtgg aatgagtatg agtaatagca gttatatctt caaaagtttg
     2041 atacaaaaca acatgtgtgg ggagatctga agatggtttt aagctatctt cttctattat
     2101 atttcccgac aaatctgtta ccaccatttg atctgcttgc atgcattcat atctgacacc
     2161 gcttggctta atgacgatga ctcctagctc acggtccact tcactcacat tgccccaagt
     2221 caatttgact aatccgtatc gcggcaatgc cagatttgct tcaaggacac gttgcttcat
     2281 tgctgcaatt ttttcttcac aagacatcat agccagcctc cttcaattta gaacttaagt
     2341 agtcttttgc ctgctggatt tctcttcgaa agtcttggct tttttcactc cacatttcaa
     2401 tcagaaatgt tccatcataa tctagacgtt tcaaatgtct taataatcca agaaaatcaa
     2461 cacaaccgct accaaaagga acatcacgaa actggcctga atattctttt gttacagggt
     2521 aagtatcttt taagtggata gcagtgatat aatcgatccc tttctcaagt tctcgagccg
     2581 gattattttc cggccatgcg gacaaattgc ctaggtctgg atatacttgt agaaaaggtg
     2641 aatgaatctg ttctttgatt tctaaaaatt ttgaaatgga gttcataaat ggatcatcca
     2701 tgatttccac ggaaagaatg atgccatatt tagaagcttc ctttacgctt tcttttattc
     2761 cttcgataaa gtcttctctg gaattcattg atttctcttc ataataaaca tcgtatccag
     2821 ctatttggat tactttgacg cttagattat gcgccaaatg gattgctttt ttcattagtt
     2881 gtttcgctgc ttttttcttt tctggatcag cagaaccaaa cggaaatcgc cgatgcgcac
     2941 ttaaacaaat cgaatggata cgtacaccgg agctgaacat ctcttctctc agctcagcta
     3001 tcttttcttc agaccaatct aatcgtgcta accgctcatc cgtctcatca atcgacatct
     3061 cgataaaatc gaagtccatt tcttttacaa gggaaaaacg ttctttccat gaaatatctt
     3121 tgggtaacgc tttttcataa atcccaattt gagccatttc cttaccccca gatacgaatg
     3181 atctcatctt tgaagttttg tgcggccttc atcggatcat ttgctgctgt aataccacga
     3241 ccagtgataa aggtatacac atcgatccct tcaaataagt gtaatgtgtg tggatttaat
     3301 ccaccagtta cagaaacatt aaagcctagt tcaatcagtt ttttgatctt cgataaatct
     3361 ttttcacccc aagtttcacc agataacagc gcatcacggc tttgatggta gattacctgc
     3421 cggataccga tgttatgcca atccatggct tgttcaaaag tccaattgcc gtagagttct
     3481 acttgcaatt cacctatttc tttttgtgct gcctccatag ttggaatcgt tgcacaacaa
     3541 atgactgtca tccagtctgc tcctgctttt gcaacattac tcgccactgt tcctccagcg
     3601 tctgcacatt tcgtatcagc gactagtttt ttatctggga acatactgcg aatacagcga
     3661 atcgcttttt gtccttcctg taagcaaaga attgtgccca cttcaataat atctactaca
     3721 tcaccaactt tcataacatc tcctaatgca tgttctaagt cattgtgatc taacgcaatt
     3781 tgcaaattcg gcctgctcat cttagttcct tcctttactt gtttttttgg taccactttg
     3841 tttcagcaaa tttttcactg atttctttga ctgacatcac atttttgata ccgataactg
     3901 taactccttt tttctctgcc tgagtaaaca tattcaaaaa attgactggc gtaaaaacta
     3961 catcgtattg gccagctgaa ctttttccct ctgaaatggc acaatgatgg attttggtaa
     4021 ttggtacgtt tagttctttc attgcttttt ccacacttct catcatcatc aagcttgttc
     4081 ctgatccatt tgcacacgat actaaaattc tcatcttaat ccctccatta tgagtgttgt
     4141 ctaatcagat tttcttttat actctctgct tttctgcata tgtgtcatag tcttctacaa
     4201 tcaaaaaata accatcagga tttttccgat attgtaactg cggaatagcc aataaaccaa
     4261 tgacaactac ggcaattcca gcataaccta ggactttcat gattactgta aacaatggcc
     4321 aagctgttgc ccagtcgaac atcccgatat aaccaccgta tgttgacatc ccaatccatg
     4381 tagcgaataa tgctgaaccg cctacttgta tgattcctga aagaaacggg aacaggcaag
     4441 cggctttgaa tccacctcgg ttattggcat aaactgctat aacggcatta tcaaagaaca
     4501 acggaatgaa accggctatg acgatcgttg gtgatttcaa taaaatcagt aaaccaatca
     4561 tcaaaaactg tcccaaagca ccaaacaaga acccaatcgt taccgcattt ggcgaaccaa
     4621 agccaaaagt agcagctacg tcaattcctg gaacagctcc tggtaaaatc gtgtttgaga
     4681 ttccttggaa agattgagtt agttcatcga cgaatgtccg aacacctaat tgcaagatcg
     4741 ccagatatac cgcaaaattt aaagaagtcg tcataatgta gaataagaaa ctctgccctt
     4801 cttgcatgaa ttgcgcttga atcaaataat ctttgcctag tacaatcaga atgatcccaa
     4861 agaaaaatag catgagtaac gaagtagcaa ccatattctc attgaaaatc gacaagaatc
     4921 ctggtaattt gatatcttca atttttttgt cttgcttgat tgatctattt ttcttttcat
     4981 cccgcttttt catccactca gctaattttg caaatataaa gattccgaac atttgctgat
     5041 gtgccactgc aaatccggca ccttctgtca attcctgcgt gattcctact gttaagttag
     5101 aagctactgt ccaataacaa cctaaaatca gtcccataaa aatcaatgct tgtatcctac
     5161 ctagatcagg aaagcaaaac agtaaaatcc aaaaagcggt agctgcttgt tgaacctgaa
     5221 cgtttccggt agtgaaaacg gaacgcagtt tcgtatattt ctgaaaacgg actaataaga
     5281 tatttaaaac aaatgcaatc agtaataaaa tcatcacatc tccaaaggta cggccaaatg
     5341 tctccattaa tccagcatcc acagcatttt gtccaaaata aggatctgta accatggcat
     5401 ccaaattgaa ccgttccttt agtccaacca agattggccg aaaattattg accaaaccac
     5461 cagagccaac agtcaaaata aagtaaccga ccgttgcctt taagaatcca gcgatgcatt
     5521 catgaagcgg gcgtttcagc aaaatatagc ctaataacac gataaaacca atcaggtatg
     5581 ctggctgcgt caagatattt gttgcaaaat acgtccaaat gcttaacaaa aattccccca
     5641 tcttttttct cctctatcaa ttaattatat tctgccatca caacttggta atcatcgatg
     5701 gagttcgtag agatgagacg ctcaaccaat ccatctgtca ttaataattc ggataatttt
     5761 tggatatttt ctagatgttc ttctggatct tttgctgcga gtgtaaaaaa caaactagcc
     5821 gttttctcct cattctcccc ttcactttca aaacggattg gctgtttcaa cttggtaaat
     5881 gaaatagctg ttccaaaaac accatcactt ttttctgaag aatgcggcat tgctacttga
     5941 ggtgcgatca caatataagg accgtatttt tctacacaat ggatgatctc ctccacatac
     6001 gtttcatcaa taatttcctt atcgatcaat acaagacagc tttcggcgat agcttcacgc
     6061 caatccgtga tttctttatc tgggtaatga atcaattgat ggtcaaaaaa gtatttcagc
     6121 aagaaactcc ccttctttct tataaaaacg acggaaacgg tgtatcattt tcaatcgaga
     6181 agacatcttc aaatcctcga taaaaattaa actcaagatc atctttgttt gtcggataga
     6241 tgaatttccc gcctacttgc cagatataag gtttaaatcc atacttcagg cgatcctttt
     6301 tgaatttcca aatttcagtg atctcttttg gatcagccat aaaattagac caaatatcgt
     6361 agtggacagg aatcactact tttgcattca atgactccgc catgcgcagc atatcaacag
     6421 aagtaacttt gtctgttatc cctcgtgggt tttcaccata ggcccctaaa cagacatcga
     6481 ttttgtgttc attaccatgt ttggcaaata aattggagta atgtgaatct cctgcatgat
     6541 aaagattccc tcctgatgtt tcgaataaat agtttactgc aatttgatcc atctcctgag
     6601 gcatttttcc ttttaatacc tcttctggat cttcacaagt gaccaacgct gtccgatcaa
     6661 acgcttctaa agctactaca ttgatgtctt tgattttgac tgcatcacct ggctttactg
     6721 taatcgtttt ttcttcagga attccccatt tttgccacgt gcgaaccact tcttttggcc
     6781 caatgaatgt tgcttcagga caattttgat gaactgctgc ggctgtatgg atatctagat
     6841 gatcggaatg gatatgtgta acgattaaag cgtctacatt ttttatcgca aaaggatcga
     6901 ttacgaaagg ctgagtacgt aaattaggct gcatattttg aacgccactc atacgcatca
     6961 tctgatggcc ttttttcatc atgccattcc catgactacg ttttcccgtc ccacaccata
     7021 gatcacataa aatattcgtt ccttcatgac ttttcagcca aattcccgta cagcccagcc
     7081 accacatagc aaaagttcca ggatccacta cttctgtttc gatttcttca ttcaagtatg
     7141 tgccccactc aggaaatgtt tctactaacc acttttcttt tgttacttca ttgacagttt
     7201 tactcatttg cttgctcccc ccatatcgtt gttccaactt acaagtaaat attaatgtgt
     7261 atcagcatcc atgaaaaccc ttttatatga ccgtttgttt tttatatgat tttctttcag
     7321 taatcaatga ttgtttcagt ataataaaga cagatatgaa tgtttaaatg aacaactagt
     7381 caaataaatc catggatcaa gatgtccctc aggaggcaaa agtcatgaaa aactcacttg
     7441 tcaatattga aaaacgtcaa aaagaaattt tggaactgct tcagcagact gaacaactat
     7501 caactttcga attagctgaa tcccttgatg tatctattag tacgatacga agagatttaa
     7561 atttattaga agaaaaaaac gagatcatac gcaattacgg ttattgttct tataattaca
     7621 aaaaccagac tgattttgat caatctggtc ctgaaaggat taaacaagcg attgccagag
     7681 aagctagtaa atacattaac gattatgata cgctgtttat caattctagc tcgactgcgt
     7741 tgaaaacact ggattttctt caatctaacc atttgacagt cattacaaac aacgtgaaaa
     7801 tcaattactc tccgcacaaa gataattgta gttatatttt aactggtgga gagatgcgct
     7861 ttccaaaaga agcactagtg ggcgacatcg cattaaatac gatttcttca gtcaatgcag
     7921 acatttgtat cattggctgt agcggagtcg atgtagtaca tggggtcaca acaaagatat
     7981 taaatgaatc aacgatcaac gaaatgatgg tcaacaagac aatcaaatgt aagattttag
     8041 tcgctgatca tcgaaaaatc ggactgacat caaagttcaa gattgctgat atttcagtct
     8101 ttgattattt gatcactgat aaattttgtt ctgaaaaagt attagaggag atcagaaata
     8161 cggggataaa aaccattcaa gtagattcat tttaagaacc aggacgtaaa tatgtaaata
     8221 ggattatcaa aaaaatgaat aacaaattta tcgataaatg aaaaacacgc tacgctcaaa
     8281 ttgataaagg aatcccaaga tgtatccatc caatcgatac catcataatc tattgcttct
     8341 tttataaccg ttatatgttg cctaaatagg aatcaaacaa tatcgaaaaa aggaggtatg
     8401 acaaaagtca cacctccttt tttcgaataa acggtattca aatcagaaca ctgatttcac
     8461 aaccacttta tttcaggtcg ttttcaatca cctttaccgc gtaatcattt ccgttgaaac
     8521 gcgctatttt ttttgcgtcc atatatcttt ctaaataatc atcttctcct aattgcttat
     8581 ggcaagaagc aatttcataa agcaagtatt ccaatgcata agtcgattta tgtttcttat
     8641 tatactcaat acccttctca cataattgga ttgctttatc ataatcctcg attttacggt
     8701 aataggtggc gatatcataa tatacaatgc agaccatcag attatcaaaa tcagtctcac
     8761 tcaatatttt aagtgtacgc tccacataaa ctttcgcaaa atctaattct ccttttgaca
     8821 agtagatcat tgccacgttt gcagctgctt gtaagtgatg gatatctgtt tcggtcgttt
     8881 gttccaacac ttgattaaaa taaaataatg ctgcatctgg ttttttcgca taaagatgat
     8941 caagcagtcc ttgataaaat aaaatttgat ttaaaagatc agaattactt actttttcct
     9001 gatcgatcgt ttgtaaaatt ttataagatt cttggtattt tgatacagaa ttcaatgatt
     9061 gaatctcatt tatttttttc ctgattttgt cttcttctgt ttctactaat atatcggaga
     9121 tctcgacatt cagctttttg caaagcgcac ttaagatatt gatgcctggc gacatattct
     9181 tgttttcgat attgctgaca gttgcttgtt cacaaatgcc ttctgctaat tgtacttgtg
     9241 ttaacccctt tttctttcgg tattctttta gtaatgctcc atctacttta atcaaaacaa
     9301 tttccttccc tttaaaaaat tatttctcct ttatatcata cacaaaaaaa agttctaaac
     9361 aaccaaaaat gtttagaaca gaataaaaat tttaatttta aaataatatt caaattaaac
     9421 aactggcgta tctgctcctt gatttctttt tgtgttatct gaataattat cgtttacagt
     9481 tataacacat agtgaaaatt gtcatggcga gaaagtctaa gaatattttt aaacataact
     9541 atttctttct tacgtgtgta taatgacgac agcacaccta tcaattaata taattatttt
     9601 atttttttat gttctcatgt ttatttttaa tgacacaact tctcgtattt atctggcaca
     9661 acatataaat cgatcaaaaa attgttgcgt gtttttttga aaattggagt ttcaacttga
     9721 ttacaaaatt taaatttaga agttgtgaaa acagcgagag acagtttaat attcaaaata
     9781 tacataggct actcgaacgc tgtgtttttt agtatactag taagaaacga agggatagac
     9841 tcattccaat aaggaggatt tcatgaagac aattcttttt gctcctgcag tattcaattt
     9901 agctgagaca acacgaatga ttgaggttgc caaagctatg aaagaccgtt attactgtgt
     9961 attctttggc ttttctaaaa cttttgctcc tttgatcaca gaaagtggct ttgagtatca
    10021 tttactagcg ccgatcctta cgaaaaaaca agaagaacaa atcataaaat ttgaccagtt
    10081 gcgtacactt aaaaaccctt ttaccaaaga aatggttgcc tctcgtatca ctagtgaaca
    10141 gagtctgatt gctaagtttg agcctcgact gattgtgaca ggttcgaatg tgacaatttt
    10201 tttatcggcc cgcaataaaa agattccttt agtatatgtt aaaccctatg ctttgagtga
    10261 accttttttt caatcagagc atgcgctgat gccccacgag ttacgtttct tcggcccttt
    10321 aagcaatccc ctttggcact ggacaaaaaa gcgtatagta aatacccgat ggatccccaa
    10381 tgccttcaaa cacgctgcac aaaaagaaat gcttccttta ccaaaaacaa gtttacaaat
    10441 gatggatgct gatctaaatc tcatcacaac gcctaaacta tttttggaag aagaacagct
    10501 gccggaaaat tatcgaatcg ttggtccaat ttttgctaag ctcagtactt ccttgcctga
    10561 agaagtgcta acttttatac agcgaaagca acaagaaaga aaacgtatta tctatttcgc
    10621 aatgggtagt tcaggcaatc cgcgtatgtt acgcaaaatt ttgttcttct tacaaaaaca
    10681 accggatcta gcggttattg cacctattga ctatctgctt gatgacattc aatctgactc
    10741 agaagatctt ttaataacag aatatctgtc tagtcatctt ttgcatgaat tcattgattt
    10801 ttcttttatc cacggcgggg aagggaccgt ccaaactgcc tgtgaatccg gcaaaccgtt
    10861 tatcggtgtg gggatgcaat ttgagcaaag ctgtaatatt gactattgtg taaaatacgg
    10921 aaacgcaatc gccctcacaa aaccagtgac tgaaagaaaa ctgcagattg ctctgcgcga
    10981 agtttcttca cccaagattc gtcagcaggc acgcacgctg caagccgttt ttccaataaa
    11041 tggtccacaa caagcaattc aagaaatcga aaccttcctg agcaaccacc caatatagtt
    11101 tcaagtagaa aaagcttttt taatagagac tcatcagtaa tttcgctatt cgagacccag
    11161 acagtgaaaa tacaaaaaaa gtaaatagtg gattgaaaag ttagagttac ttaaattacg
    11221 gtgtctaaaa aacacaaaat catccttaaa ataaaagatc tgacgctatc caaatgagca
    11281 taggtagcgt ctgccattta ttcttagtca aattctttga cacctttctc tggttggatc
    11341 atctcaacgt ttcttcaaaa gaaagaccat aataattagg caaatacatt ttcttaaaat
    11401 gtgtcatata gatctagaag catcgctttg tagagatttg tgccgctagt cccctcaaca
    11461 attactaaat caaatgtagg atttaccact ttatctttga taatcaacct ccctcttctg
    11521 ttgagacaat gatctcgcaa tccgatgcat actcctatat aaactgccag caatccccac
    11581 atattgagta gatcatgtca gaatgttcta tgtcgtcagc aacgatacac taaaagtcac
    11641 gttcaccatg actgattgct attcccagca caattatttc tatacacgtt tcatgatttg
    11701 aataaacact cacatcatac atattaccat tcatacacca tactgcagcc cctactatat
    11761 ggttgtaagc tcccaaatct atatttgact tgatcacttt ggtagtagat ctactaattc
    11821 taaatccatt gctgataaaa tttccatttt ttcctccttc tttctgccaa aacagttaaa
    11881 acaataataa tctttatgta aaaaagggct gtgacaaaag ccttgtcaca gtcccatatt
    11941 ccgaataaac ggtggtcaaa agcttgcatc tgcggtaata agttcaacca aacgaaaaat
    12001 aagccaacat ttcacaaaaa tttaagaagc aattttcgta aatttcttct tatttcttga
    12061 agctaaccac ttctgttaca accactttta gaacatttaa agcttcgatg tgacaaacca
    12121 tctatgctgc tcgatcataa aattgatagc aaaaattcca caaagtacat atgtccccca
    12181 tgaaaacaaa acattcatat gcggtacaat gatctttgga agaatcacga tcaaaaatga
    12241 tgccagtgcc caaatccatc gattgatttg aatatgatat gtagctagca tattcaatat
    12301 aaggtgacta gccgttaata aaaagataaa aacaaaaaat aaaatcagcc aatacattgt
    12361 ttcccacctt ctaaaaacca aacttagaca gtctggactt cgttttctcc acgactttgt
    12421 tcatctgaca gcatcttttt atcataagct tttacaaatg gataatagat aataaatgct
    12481 atcaatgtgt tgatcatgat caagataaca acacgccagt catttcctgc tgaaagataa
    12541 ccgccgattg gagctggtaa tgtccatgtt ggcaaaacag taaatctatt gaccatttct
    12601 aatttgactg caaaataact gataatagtc aaaactaaag gaactaagtt gaacgggatg
    12661 aacatgtaag gattcaacat cataggagct ccaaaaatta tgggctcatt gatattgaag
    12721 ataccaggaa taatagaaaa acgaccaacc tctttcaaga attttgattt acagaagaat
    12781 ataaacaaaa cagataccac gatcgttgct cctgcaccac caattgttac cgtccattga
    12841 taaaactgtt caggagcgat atagggtaat tgggtggcag ctgtaccatc tgccaatgcc
    12901 tttgcattct catccaacat gatcagccac attggacgaa caacagcccc tacaatactc
    12961 atgccatgga taccaaatga ccacaacaaa tggatcaaaa acattggtag taaaacacca
    13021 agcaaattat tccctaaaat attggttaaa ggctcgaata acgataataa ggcagcgttt
    13081 acatcaaagc ccgcgacgct tcgaatcagc caagtaattg ttaaaatgac tgcacctgga
    13141 atcaatgcct caaaactacg ggcaacagca ggtggcactt gttctggcat tttgatagta
    13201 atatttcgat ttttaaagaa acgatatact tccactgcaa aaatcataga taagatcccc
    13261 gcaaacatcc cagatgctcc caaagaactc ataggtaaga cccagccaag aggtgcctct
    13321 tcaggagtta aaacgtttac aggaacttgt aacataaaga acgttcctaa agataatata
    13381 ccacctgtca cactatctaa tccatatgat ttagctagat ccgaacccat aacaaaagtt
    13441 gcataaatag ccatcaatcc catcgtcatc ctaaagggta aaacaatcgt tgccgaataa
    13501 ggggcaataa attcattcca tccaggaaac ggtaaattga taaagaccat aaaaaatgtc
    13561 ccaatcaaaa tcaatgacaa tgttgaaaca accccattac gaattgctaa taaatgacgc
    13621 tgtgtaccaa tttttgccat tggtgggaca agtttctctt cgacccattt catcagcttt
    13681 tccactaaaa ccactccttt tcaataaaat atgaaccatt cattgcatga caacgcaaag
    13741 aatgttcata actaattata taattaagaa attttatttc aatatattct cttaaataat
    13801 caaatttatt tttaagaatc ctgaaaataa gttttctaaa tcataggagc aacaaaaaaa
    13861 ttcgataatt aacaaaaatc aaaatgatta aatctagtga ttgaaacata tcatttcaaa
    13921 aaaattttgt aaattcaccc ataaatatca ccctatttaa cttttttcat cgtacactaa
    13981 aattcaaaac aactatcttt aatctttact tcttggtttt gttttaataa atttccttca
    14041 aaaatgcctg gagaaactac aatgatcacc gtatcttttt gtgcttgctc caacttcttc
    14101 aaatctataa tagctaacag tgtcccttct tttacctcct ggttactttc aacccgtatc
    14161 tgaaacggac ttccttcgag ttcaactgta tctaaaccca tatggatcag tatttttctg
    14221 ccagattcac tttcgatcgt tatggcatgt cgggtaggaa atatattatc gattattcct
    14281 ttaactggcg catggatata tccatcatga ttcgttatag cgtatccaac tcctatcatt
    14341 tttgaagaaa agacctcgtc ttttactgct gttaatggaa taatttgacc actagctaca
    14401 gcttttagaa tgtttcttgt tttaaagaaa tgcacttttt atcacctcta ctttaatgct
    14461 ggccaatatt ttttgtttac tttgatcatt tcatctagta ttttttttgc gactctggca
    14521 tttggtacgg tttgattcaa tgtaaatgct tgtaaagctt tttgataaga accttcaaag
    14581 taagcatcta ctagtaactt ctctactgca acttgtgcct ccatcaatcc tttatgaaaa
    14641 tcactgattg gttttcttag tgtaattggc tccacaccag ttgcaccaac atatgccggt
    14701 atttcaacaa ctgcatctga ccgtagattc ggaatcgccc cttgattagg aacgattagc
    14761 ataaagcgac gacgatcatc attcaacaag gaaatagcta tatcgacgat atattgtcca
    14821 tgctccccaa aattaaagtt caatacttct tccacaatac cgcggcgtat tttatcagcc
    14881 atttctttgg tatttttctc tcgtccatcc atgaccatat tagcacgtgt atactctttg
    14941 tcagtatgct caacgaccat atcaggatat aagtaatact ctaagtaatt attcgggata
    15001 ttacttggaa aatttttcgt gatgactgac atcatttgaa aggttttttg ccaagtttta
    15061 tctcctatat taaaatcggc gacttgcatt tcttttgtta ttagtttttc aataatctct
    15121 ggcataattt ctcgttgcaa ttctttatca taaatcgatg tataccagcc aaagtggttt
    15181 aaaccataat acgtaacgat ccaattctta cgatcataac cgtaattcac tgcaatcgtt
    15241 tcttcaattg aaatagtcat atcacaggca ttaatcattt ttacattcgg atatttacga
    15301 cgaacagctt cagaaacgat cgactctgga tttgtataat tcaaaatcca tgctttgggg
    15361 gcatagtctt gtatatgatc gacaatttcc aataaacctt tgattgagcg catcccataa
    15421 gcaaatcctc cgagtccaca cgtttcttgt cctactaatc catgtttaag tggaatcttt
    15481 tcgtcctttt ctcgcatctc caatcctcca acacgaattt gtgagaaaac aaaatcgcat
    15541 cctgtaaaag caagctttgg atcatccgtt gcacgaatgc gaatatttga aaaacctttt
    15601 cttttcagca taaatttaac aatcaagtac atatccatat ttctttcttc atcaatatca
    15661 tataaacgta tttcagataa tggcaaccgt ttttcattgt tcaaaatggc ttgaacgatt
    15721 cctggtgtat acgtactccc gcctccagct atagtgataa tctgtttttt catcatcatc
    15781 ttttcctttc tattcttcta ataatttatt gaattctgcc ataacataag tcacttctaa
    15841 gccgatgata atttgataat ttttttcagt tggtttaatg atcccgctta tccctaattg
    15901 cttgattcgc tgttcctcga ccatggtggg atccactacc tctacccgta ggcgtgtatt
    15961 acaactatag acatccagga tattggcttt tcctccaaac gcttgtagca tttttgttgc
    16021 catatattca taatttttac ttgctaaccc taactctgtt tcttctaagg ttacttcatt
    16081 gccaaactct gccttagctt cacgtcctaa tgtctgaatg tttctcttat tgatcaaata
    16141 ataaaaagta acgtaataca aggcaaaaaa gaaaattccg attggaagaa tcaaaatagc
    16201 attggtagcg attcgaaaat tgatgagata atcaacaata catgctccga atgtaaaacc
    16261 aattcgtata ttgaataagt aacagacaat ccctgctaaa ccggcaaaaa gtgcatggat
    16321 cacgtacaac ataggagaaa gaaacatgaa actaaattca acaggctcag tgatatttgc
    16381 aataaatgaa gttcctgcac cgcttcccat gattcctttg accatctctt tattttcttt
    16441 ataagctgct ttatagatag ctagacatat agctggtata ccaaacatca ttactacaaa
    16501 aaaaactgat aaaaacagtc cagcctgagg atctccgttg ataaagcgtg tcatctctcc
    16561 tctgaaaact tccttgctag gagatgtata gcttcctaaa tcgtaataaa tatacgtatt
    16621 caacacatgg tgcagtccaa aggggatcag caaacgattc aaaaacataa atatccccac
    16681 acctaatgct cccatatcaa cgagcagttt tgcaaaatta tcaatcccaa tttggatgag
    16741 cggccagata aagcccaaaa aaacagctgc gattgtttgt agaatcatca ccatagtcaa
    16801 tgggaatttt tcgcccgcaa aataagaaaa aactactggt aatttttgtt ctttaaagcg
    16861 attatacgtc catgccgcta ctaaaccgga aataattcca ccaaaaatcc ccatattcac
    16921 ttctgggtcc ataatttcta agcctttttt caaggtagca atcgctaaaa aaccagtaag
    16981 tgcaggtatt cctttgtcct tatttttgac aaaaccgatt gcaactccta tagcaaataa
    17041 gatatccaag tttccaaaaa cggcatttcc agctgcacca attactggga tgttcagcat
    17101 atcgtcagct gataaacgca tgatcaatcc cgctaatggc atcgctgcaa ctggaagtag
    17161 catcgctctt ccaagcttct gcacatttga tttaacattc atttttattc ctcctctatt
    17221 aaatatcctc atatgttact aactcaatca aaggatcatt tttcactttt tgagcaaatg
    17281 ctgaatgaat taaaaattcg acttggtggg tacgaggata cacaaaactt gaaccattta
    17341 acaattcctg atccaaatac gctgcgtgac acatgacttc gatcgtttta tcttctctca
    17401 atatcatgag taaacttgcc aaatattctt ccacactcgt attgatttgt cgtttttttt
    17461 catcagtttc tcctacgaca tcgaaacaag gctcaaaata atcgacatga cgaacttcat
    17521 cttttcgttc aaagttgctc cgaaccggca aatcatattt ttttgctaat gtaacaacta
    17581 cctcctgatt ttcatccaaa gtatgagtat gatgatgact gtctaaatga gttggtatga
    17641 tccccgcatg ataaattttt tgaatctgag cattccattc ctgatacagt tgatctttgt
    17701 caatgttaaa tggatgctga taataagata aggatctgaa ctctccgctt tcgataagag
    17761 tatcgacagt tttgaggagt ggtttgccac acgtcaatgt taaatggact cctacaccta
    17821 gcctaggcat ttcctttctt agtttcacag catgctcgaa acccggcata tttgccatca
    17881 atgtagttga tgtcaaaatt cctcgctgat aagcatccat aatcccataa ttcacaccat
    17941 ggctatagcc aaaatcatcc gaattaaaaa tcacttttcc catttcattc tccttctttt
    18001 ttctactaaa ataggcgcaa ccaaataatc atagtccatt tactatgtgt catttggttg
    18061 cgcctataga tagttacgcc cttattttaa tagattcttg ctagatgaat tgccaaatag
    18121 atgatttcat tttcttgaat ggcaagttga aattgctgtt cgatgatatc tttaatcttt
    18181 agtgctgtac aatacgcttt gctatgttta aatgtttcag taaaaaaacc aatattttct
    18241 actggcgttt cttcctgata aatcaagcgt ttaaccagga atttgatatg aaccattaag
    18301 cgatgataag tcagtgattc tttatcataa acgtttgaat aggatttttc tagatgggac
    18361 aaaatcgtgg tcgtcatttc tactattttt aagctcttta aactattagg ctctcctagc
    18421 tcagcattaa tgatatgcat agtgatgaat cctacttcat ttgaccctaa ctcggtttga
    18481 tatcgatgat tgattcgctc gaccacttct actgctatat catattcatt tggataaagg
    18541 aatctcattt ctggcaacat aattgctgtg acatctctat cttctctttt tcgttgaata
    18601 gcaaaataaa tatgatctgt caatgtaata tagatatttg aatctaatgg agaggagatc
    18661 ctagctgtta tcagttcaat cacttcttcc gcaaaatcca taaattcgat tggaatctgc
    18721 gagactatct cactgaatcg tttgatcttt tctttagaat ctaatacaaa taatttgtca
    18781 gcatcctcaa catcaaatgg cttactcact ttagcagaaa agccaatccc tttccccatc
    18841 gcaatttttt ctattccatc gtcatcgatc aatacagcat tatgattcag ctttttcatc
    18901 acatacaccg ttaattcacc tcctctatat gataaaacaa aacaacttgc agactccttc
    18961 atacatattt ttgtctccaa acaattttta aagacaaaaa acgacctaag aaagaaccct
    19021 ttcttaggtc gtgcctaatt gaatagtagc accctacata atttacaaaa atagtttaac
    19081 acataataaa agcgttatca atatcttttt tactcgtttt atgataatac ttgtatcgaa
    19141 ttgatttgga ggtaattaca taaaaacaaa aaaaatatag caactctcta taagaaagtt
    19201 gctatattga taattatacc ggcggccggg gtcgaaccgg cacgtcctca acggacactg
    19261 gattttgagt ccagcgcgtc tgccaattcc gccacgccgg caaatttaaa aataataact
    19321 ggggtagctg gattcgaacc aacgcatgag ggagtcaaag tcccttgcct taccgcttgg
    19381 ctatacccca agaataaagg gcgaatgatg ggaatcgaac ccacgaatgc cggagccaca
    19441 atccggtgcg ttaaccactt cgccacattc gccatcataa aagatgttat ttaatttctc
    19501 aataaaaaac taagatgaag acatcttagt caataccggc ggccggggtc gaaccggcac
    19561 gccctcaacg ggcactggat tttgagtcca gcgcgtctgc caattccgcc acgccggcaa
    19621 gttgatttat tagtactaag gcggtaaccg gatttgaacc ggtgatgaag gttttgcaga
    19681 cctctgcctt accacttggc tataccgcca ttatactatt atcaatctat aaaataaaga
    19741 ataaattata gaaactgggg tagctggatt cgaaccaacg catgagggag tcaaagtccc
    19801 ttgccttacc gcttggctat accccaagaa taaaaagggc gaatgatggg aatcgaaccc
    19861 acgaatgccg gagccacaat ccggtgcgtt aaccacttcg ccacattcgc catggcaggg
    19921 gcagcaggaa ttgaacccac actaacggtt ttggagaccg ctgttctacc tttaaactat
    19981 gcccctataa aatggaggag agtggattcg aaccactgaa ccctaaggaa cggatttaca
    20041 gtccgtcgcg tttagccact tcgctactcc tccatggtgg cgcgggacag aatcgaactg
    20101 ccgacacacg gagcttcaat ccgttgctct accaactgag ctaccgcgcc atattttaaa
    20161 attattaatt gaataacaag aacgacggtt ccgacgggaa tcgaacccgc gatctcctgc
    20221 gtgacaggca ggcatgttaa cccctacacc acggaaccag ttcattatgg aggttaacgg
    20281 gatcgaaccg ctgaccctct gcttgtaagg cagatgctct cccagctgag ctaaacctcc
    20341 aacattcaat taaaatgacc cgtacgggat tcgaacccgt gttaccgccg tgaaagggcg
    20401 gtgtcttaac cgcttgacca acgggccagg taaatttgct acggagagta agggattcga
    20461 acccttgaga cagtttggca ccatctacat gatttccaat catgctcctt cggcctctcg
    20521 gacaactctc cagagtgaaa gaagctcaat actctttctc aaaatgagaa ctccggcagt
    20581 aggactcgaa cctacgacat catgattaac agtcatgcgc tactaccaac tgagctatgc
    20641 cggaataaca tcagcgtggc gacgtcctac tctcacaagg ggcaacccct cactacaatc
    20701 ggcgctaaga agcttaactt ctgtgttcgg catggttaca ggtgtatcct tctcgctatc
    20761 gccaccacac tgtggtgtta tcttttattg agtaaatttt gttcactcaa aactggattt
    20821 gaagtcacat aagttt
//
LOCUS       DACDIE010000046        20172 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.046, whole genome shotgun sequence.
ACCESSION   DACDIE010000046 DACDIE010000000
VERSION     DACDIE010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20172
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.046"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            273..1751
                     /locus_tag="IV250_001926"
     CDS             273..1751
                     /locus_tag="IV250_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP8769190.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            2028..4088
                     /locus_tag="IV250_001927"
     CDS             2028..4088
                     /locus_tag="IV250_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769191.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            4171..4740
                     /locus_tag="IV250_001928"
     CDS             4171..4740
                     /locus_tag="IV250_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8769192.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(4915..5721)
                     /locus_tag="IV250_001929"
     CDS             complement(4915..5721)
                     /locus_tag="IV250_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769193.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            5859..7373
                     /locus_tag="IV250_001930"
     CDS             5859..7373
                     /locus_tag="IV250_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HAP8769194.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(7776..8759)
                     /locus_tag="IV250_001931"
     CDS             complement(7776..8759)
                     /locus_tag="IV250_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HAP8769195.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(8764..9411)
                     /locus_tag="IV250_001932"
     CDS             complement(8764..9411)
                     /locus_tag="IV250_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8769196.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(9496..10128)
                     /locus_tag="IV250_001933"
     CDS             complement(9496..10128)
                     /locus_tag="IV250_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP8769197.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(10144..10695)
                     /gene="coaC"
                     /locus_tag="IV250_001934"
     CDS             complement(10144..10695)
                     /gene="coaC"
                     /locus_tag="IV250_001934"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HAP8769198.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(10692..11429)
                     /locus_tag="IV250_001935"
     CDS             complement(10692..11429)
                     /locus_tag="IV250_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HAP8769199.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(11426..12415)
                     /locus_tag="IV250_001936"
     CDS             complement(11426..12415)
                     /locus_tag="IV250_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP8769200.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            12673..13215
                     /locus_tag="IV250_001937"
     CDS             12673..13215
                     /locus_tag="IV250_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8769201.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            13232..14317
                     /locus_tag="IV250_001938"
     CDS             13232..14317
                     /locus_tag="IV250_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8769202.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            14329..15135
                     /locus_tag="IV250_001939"
     CDS             14329..15135
                     /locus_tag="IV250_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8769203.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            15136..15963
                     /locus_tag="IV250_001940"
     CDS             15136..15963
                     /locus_tag="IV250_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8769204.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            15960..17033
                     /locus_tag="IV250_001941"
     CDS             15960..17033
                     /locus_tag="IV250_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8769205.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            17461..20157
                     /locus_tag="IV250_001942"
     CDS             17461..20157
                     /locus_tag="IV250_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8769206.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
BASE COUNT         6603 a         3496 c         4060 g         6013 t
ORIGIN      
        1 aaagagcagg acggtggccg tcctgctctt ttttgtggtt gtttacgcaa atggcgcaaa
       61 tcttgtacat tttaacacaa taaaacaaaa aatatagaat atttcctcat aaatttttcc
      121 attttttgca atattctcaa aaaaaagata aaaaatgaga ataactgatg aaaaaacaag
      181 cataatccat cataaaatca gtataggtga aaagatgaac tttcatctgc atatcaagta
      241 attggtatga ggcatttttt gaggtgtagg atatgaagtt ggattacctt atagaagagg
      301 aagatagaga aaaactgctg ctagctagat atttagaatt tgagaaaaag aattatgtag
      361 tgctgcctaa gttatatgaa acattaggtt tgtctaaatt taaaacaaga aattatatca
      421 tagaattaaa taaagaattt actgaattca aaacaaagcc agagatccaa atttcagaag
      481 aagcagagat ccatgtcgaa cgtctgaatt tgacggatat caaaaattta agaagaatct
      541 actttcaacg atcaaaaatt gctcgactgt taacggagat cgtggattca aaagtaacga
      601 tcgaaaaatt tgcagagaat cattttttaa gcatatcaag agcctatgta aaaagaagag
      661 aattagtctc ttttcttgaa cagcatcaaa taaagttaaa gaaaaacata ttagttgggg
      721 aagaaataaa catacggaac gtattatttg gggtatattt cggggtcttt aatggttttt
      781 ctatgccatt tagagaaagc gtcatgaatg aagcaaagag tatcgtgcaa tattttgaat
      841 atctgttcaa tttaaaactg tctgcaacaa aacgaatcaa attcaatttg ctaaccacta
      901 ttagtttgtt gagggcctat gggaatcaac caatcggaaa acgtttcttt tcagatgaat
      961 actttaagag ctcagaagga atgaagatca tcaaggattt atcacatatt tcttttcttc
     1021 atatccctaa agagcagatc attgatgaga tttcttatat catgttgttt ttgatcacgg
     1081 aagaagaatt tgaattagat ggcgcatttg gacttgcgga agctcatttt catgatatcc
     1141 acatgattag tcaaaaaatg tcagtggaat tgattgatca attaccattt tcgattgaaa
     1201 tctctgaaga agaaagacta tatatacgaa aagaacttcg tatgggtatg atgcatgtca
     1261 atcgaaaaaa ttggatattc gactttgaga tgactagctt tacgtctaaa aagcagctac
     1321 agtttttctt cgagacgtat ccaatattca gtacagcaat ccaaaaggtc atacaagaga
     1381 ataaacgcta tttcttggga aaagatgaac gtttcatgat tcgcttattt tatgattatc
     1441 tctttttatt ggtcggtatt tgtccggtat catattttga agcaccgatt catgtctgta
     1501 ttgatttttc gcaaggatct gcttataccg attacattat ttctcaagtg aaaggattta
     1561 aaaattacaa tattgtgatt gagagtcgaa tcacttctcg tacgaatatt tatttatctg
     1621 attgtttact tgaaaaattt aataaagaac agataatttg gaaaaatcca ccgactccag
     1681 atgactggga atattttgga aataccatca tcagagaaaa aaatagacat ttaactgaaa
     1741 aaatcagcta gtatttatgg atggcagctt tgtctcacat atttattagc tgatttatgt
     1801 ttttttcaaa gaaaaggtaa ctagatataa ctagatataa ctagatatat tacttaacaa
     1861 aattaaaaag tacataagtt attattccga ttttcttgaa acaataaaaa atcaaaaaaa
     1921 atactctcct aatagtccat cttcttttga ttttatttat ataatgaaat catcttatga
     1981 aaattactac ttcatgttta gacaagagta gggaggacta agaataaatg tgttcaaaaa
     2041 gacgttcaaa acaactaaat aaacacgctt gtcgtttaga taaggaacgg tcgtgttctg
     2101 aaaaacacta cgttaagttt atgatgcgca cgatgctgat cagtttatct gtaggtgtct
     2161 atatgcatgt acccgttcag gcaacgacac aaaccatcaa ggtcgtacgt acctctgttt
     2221 ctgagaccac tgattcccct atcactatta ctcatacttt cactgatgca aatggaaaac
     2281 agctacttcc tgcattgact tcctataata aagtcagtta tcctccaacg ataaaccact
     2341 actggttaat tcctaacaaa acattatgga catcggaaac cggaagcagt gtgccgcttt
     2401 ctatcttttc cgatatgact gatcctacaa aaggtgatca gttaaaacag atcactgata
     2461 ttttgaatca tgctttgaca gatataaagg acggtggaag cgcacaagtt tcttatgtat
     2521 atgaaaaaga ttattcacag tttgaaagaa agaaaatcga tgttctggca gggacaaaag
     2581 tttcgatatc tgatctggta gaaaggctga agaacgcaga tggaacacaa ggagatatgt
     2641 ccaaagtaat ttcaaacaat gggaatatcg tggatacgac taatacagga acatctacta
     2701 tttccttgtc ctattttgat ccaattgctt taaaaacaat gactgcatct gctgtgatcc
     2761 atgttcgtgc tgatcaaaca gctatcaaag gaaaagatct tcacttaaga acaggagaga
     2821 gttacgcccc taaagatctt gttgaatatg taacaaaaag cgatggaact tcaggaaata
     2881 aagaagaaat caaaatgaac gggacagtag atactacaac tccgggagaa tatcccgttg
     2941 tattaactta tatagactcg aaaacatcaa atgtggcaga aacaacagct gtagtttatg
     3001 tagaagcatc tgctgcttca tcgactgtca tcactcattt tatagatagt caaacaaata
     3061 aagaacttca agcagaaaca catatgagta gtgtaaatcg tattcctgat ttgcgcctac
     3121 ctgctgaatt ttctgatcga gtgccaaata tagatttatc gacttatgaa caaaatggga
     3181 aagtcacttc tatttctgat tggatgcaag caaacggatt agacagaaaa agtcactggt
     3241 cggagatcct tgaagtactt cactcagaac tgatgaataa taggaagcaa tcaggagggg
     3301 aaattacctt tacttatgtt tatataccag acaatcgttc attagaaggt catgatctta
     3361 ttgttcatcc aaacgaaaaa ataaacaaag aagagttgat ccaatcagca acaaattcct
     3421 taggtaaacc cgtaccgtta aaggatgtat cggttacaat caatgaagta ccattggagg
     3481 atggttattt gaccaaacaa acaggtgatt ttacgattat gtatagttat catgacccgt
     3541 attcactcca agaactccat gctcagtcac tattacatgt tttaccatta aacaaacaga
     3601 tagatcaaaa agaacaaaaa agacaagaag aaagcttgtc agaaaaaaaa caacaaatgg
     3661 cccatgcagt ttctccaaca gggaaagtaa aacaaacaag aagcctagag ttagcaagaa
     3721 tatctagaaa aatctcagaa tctaataaaa cgataaggca agaagctcat ttggccccac
     3781 aagtttctgg attggaaaat gaaattttag agcctaaaat aaaacaacaa tcagaaaaag
     3841 aatttgttgc taaacagtcg gaagatcaga aggaatcaga tgaccagaag gaaacgaaga
     3901 aaaaacgctt gtcaactatt gattccaccg atgagcatct gcagccgcaa gtaccacttc
     3961 ctagtccggg aacacccgca cctggtggag gtattccttc ttctgcttta ggagatttca
     4021 tgcgaggtat ggcaggaaca tgggtatacg ctaatcgaaa tgaatttgac gcagatgact
     4081 ggttctgatt tatatctcta tctgttatag tcaaataagt agctgagtca tatgttattt
     4141 ggcataatgg agacgaggag ggatagaaaa atgttagata aaacgttacc ttacgcagaa
     4201 atctggatga caagaccact tcaacaaaat gttccaatca cgcctttagc ggaaggtttt
     4261 gaatttcgat tttatcaaaa agaagatgaa tctgcttggg cggagattga gacttccgtt
     4321 ggggaatttc aaacgacaga tgaagcactt gagtattttc aacaaaactt tgctccttat
     4381 cctgaagaac ttgaaagaag gatgtttttt gtagagacaa aaaaaggaga aaaagtagca
     4441 acctgtaccg cttggcgaaa aaaatcaaga gagcaagtgt atccggtttt tcattggtta
     4501 gcagtgaaac cagattacca aggaaaaggg ctcgcaaaag cattgactgc cagagtcttg
     4561 caagaatttc ctaccctcca tacggacgga ccaatctatc ttcacacaca gacatggagc
     4621 catcaggcga ttgctcttta taaaaaaatg ggttttactt ttattgctga aaatctagac
     4681 ggcacaccta atccagatta tgaaaaagtt atgcaggttt taagtaaatt agataaataa
     4741 cgaaatggtg aaataagtcc tcagtattgg gataaaaaac gaaatagtca ttcctcatat
     4801 cttacttttt tgagttaata ctatcggata actaatggca tgccatacat tcaaaaaaga
     4861 aggggcagtg acaaaagtat tgtcactgcc ccttctcttg ttttttagca tgttttatcg
     4921 tttgattttt ttaggaataa aattcttgat gaacgtaaat ttttggtaat cgtttcgact
     4981 atagaggtaa ccaaatacac tgaaaacaac tgctccaata aaattaacaa ataaatcctt
     5041 catagtatcc atgattccca cgtctaagta accattttgg atgattgttt tatctgtttt
     5101 accatctgca ttttgacttt caatgattgt ccgagtgata tctggaacat gatagacagt
     5161 gtttccgtct ttacttaact ccacactatt gatttgctgg atgatcgtat ctttttgcat
     5221 atcgaattgc agtaatttat ctgctgaaaa ttccacaaac tcccacataa caccaactgt
     5281 catcgaaaaa cagaaggaaa caactgctag taataaagga gtcaagttca tggtttttgt
     5341 atttttattg agcaaataaa caagggaaaa gccgacaccc ccagctaaaa atccgctgaa
     5401 tgcatgtaga aaagtatccc acattgaaaa atagccataa aaatcactta actctcctaa
     5461 aactgttgaa ctaaaaataa aagataacaa aatgatttct agcatatttg gaatctgtat
     5521 ctttaatatc ttttccagaa aataagggat ttcaaataaa attaatgtca gtaataaaag
     5581 cattgtattt ccccagtgtt ggttccagac ctgatccact agcataataa cgacgatgat
     5641 acgtaataaa atatagattc cgtttgtgat tctacctgca gtttttgtac tgttcgtaaa
     5701 aaaggttttc aaatgattca taaaataact ccttcatgta tatccttaat tatactaaga
     5761 agcgtgtgtt taccaaagct atatgactta aagaaaaaca gagttgatat tgtgaaaaaa
     5821 atcacttaat atagtataag gagaaaaggg ggatacaaat gaaaaaatta gttgctggtt
     5881 tattaacagc cgttgcactt cttgctatga gcgcatgcgg cgcagatcag aaaaatacag
     5941 cgcagacaag ttctagtacg aaggaatctg ccgaagtatc atcaggcgct tctgaacaag
     6001 tatatacaga tccaaaagaa atgaaagaat cttatgatgt cgtgattatt ggagctggtg
     6061 gtgcagggat ggcagcagct atcgaagcaa aagatgctgg gttgaatcca gtaattttgg
     6121 aaaagatgcc tgttgccgga ggaaacacat ctaaatcatc aagcggtatg aatgcttcag
     6181 aaacaaaatt tcaaaaagaa gaaggaatca aagactcgaa tgatgccttc tatgaagaaa
     6241 cgctaaaagg tggtggagga acgaatgata aagaaatgct tcgatacttt gtcgatcact
     6301 ctgctgatgc ggtcgattgg ctagatcaaa acggtatcaa actggacaac ttaacgatca
     6361 ctggagggat gagtgaaaag aggacacatc gtccgtctga tggctcggct attggtggat
     6421 acttagtgga ggggttattg aataacgtcc atgagcggga aatccctatt tttgtcaacg
     6481 cagatgtgac agacattaag aaaaatgggg aaacggtcaa tgaagtgact gtacatgttc
     6541 aaggagaaga accaaaagag gtcaccggaa aagcaatcgt agttaccaca ggcggttttg
     6601 gtgccagcaa agaatttatt gaaaaatatc gtcccgattt agctgattat attacgacaa
     6661 atcaagaagg atcaactgga gatggtatca ccatgattga aaaattaggt ggacaaaccg
     6721 ttgatatgga taaaatacaa attcatccaa ctgtgcagca ggatgagggg gtcttgattg
     6781 gtgaggttgt tcgcggagaa ggagcaatct tggtcgatca agaggggaaa agatttgtca
     6841 atgaaatgga tacaagggat aaagtatctg cagcaattac agcattgcct gaaaaatcag
     6901 cttatttgat ttttgaccaa ggcgtgcgtg atcgtgccac agctattgaa ttttatgacc
     6961 aaaaaggata tgtcacaact ggaaagacaa tggaagaatt agcagaaaaa atcgatttac
     7021 caaaagaaac actagcaaaa acagttgatg gatggaatac ggatgttgcg gagaaaaaag
     7081 atgctcaatt caaccgtgcc acagccatgg aacatgactt atcgtctccg aattattatg
     7141 cgatcaaaat cgcaccagga atccattaca cgatgggcgg tgtgaaaatc aatacgaaca
     7201 ctgaagtatt agataaagaa aaccagccaa ttagggggtt atatgctgct ggtgaagtca
     7261 caggtggctt acatggaaac aaccggatcg gtggaaattc agttgcggag atcattattt
     7321 ttgggcgaca agcgggcata caagcagctc gatttgcttc ggaaaatgaa tgaaatagaa
     7381 cgtaaataaa aagaggttgt gaaatcagcg ttttgagcta ataccgaggg tcagctgatt
     7441 gaacactgtt tagtgaagag gttgtgaaaa agctgccctg catcaagtaa taagaacagc
     7501 caagtaaaaa cagcttctta tgtttttaac ttggttggac ttattaccgc agatgcaagc
     7561 ttttgaacac cgtttattcg gaaaaagagc gtgggacaaa agtaaagaac acttttgtct
     7621 cacgctctaa acacgtataa acggcggaag cagaagaaat tccttcggaa ataagctgaa
     7681 attcacaaaa atttgaaaag caattttcgt gaatttcttc ttatttcttg gagttaaacg
     7741 cttctgtccc agcctctttt ttgatcagca tgattttatt cagttatctt caaaatgatt
     7801 tttccgatgt ttttgtttgc ttccatatat tcatgcgctt gtttgactgc gctgaattgg
     7861 aatgtacggt caatgattgg ccgaatcttg ccattttcta gaaaaggccc agctttttgc
     7921 ataaaagttt gtgcgagctc tgctttatac tgatcacttc taggtgtcaa taatgtacca
     7981 agcagttgga cacgtttccc aatcaataca ccaaggtcta tctcaggcac gatggtccca
     8041 cctagcatac caatcaagac ccaacgtcca tcaacggcta tagaagctaa attcttttcc
     8101 cagtaagaag cgccgatgaa atccaaaata acatcgactc cccgaccttc agtgattttt
     8161 cgtacctctt cttgaaagtc ttgtttttta tagttgatca attcatctgc accaagttct
     8221 ttacaaaagg cgagtttttc ttctgatcct gcagtaacga tgattttcgc gtgagaaagc
     8281 tgtctagcta attggatcgc tgctgttccc acaccacttg ctcctgcatg gatcaagaca
     8341 gtttcgcctt cttgtaattt tccaagccag taaagtgttt gataagcagt caaaaaaact
     8401 tctgagattc cagcagcaga aacatagtct agcgtatcag ggagaaggat tgcacgatcg
     8461 gcaggcatca cagcatattc ggcataacca cctagattga ctaatccgta tacttttgtc
     8521 ccaggagtaa aagtggagat atcactttta ttctcaacta cctctccagc tacttcgact
     8581 cccaaaatag ggtagggagg tttcaaagag actgattctc ttttcataat atctgttcga
     8641 ttgacagctg ctgtatggac tttgatcagt aattcgcctt gttttggttt aggaacaggc
     8701 acttcaattt gttctaatac atctacagaa ccaggatgct tgattgaaat ggcacgcatg
     8761 gatctattcc tcctttgccc attcttggaa tgctgtattc aactctttgc taccttcaga
     8821 aagtactaga gtcgtacgag aaatatccgt ttcttccact agagcggcaa gtacagcagc
     8881 aactgtagag cgagtgatcg tttttgatgc cttctcatta ggcttagtta attgaatgga
     8941 agtaacttct tcatcgttag tcaatgtgac tggttgaaga atgacaaaat ctaagttagt
     9001 tcgtttcagc cattcatctg cataatgttt agtaatataa taatctgtca tggactcagg
     9061 ccattttgct ggttcatctg cataaacagc actgaccatc agatagcgag agacatgcgc
     9121 attttcagct gcgatcactg ttttgattgc gccatccaag tctacttgca gaagattctt
     9181 tccttgagag ccggcagcaa aaatcaatac atcgatgcct ttgaatgctt cggtcatttt
     9241 ttccacggac caagttaaat caaaagagac atacgaaaca ttctcttttt ttactaatgt
     9301 ttgggaagca acatctctca cccctgcaaa aatttcatgg gaacttggag ctaaatcttt
     9361 tatcaggtgt cggccaattt gtccgtttgc acctacaaca aaaattttca tttggatcat
     9421 cctttcgacg tcaaatcttt ccattcaata gtaacaccaa aagagaaaaa aatcagagat
     9481 cggctacgat gactatcagt tagtaagaga agggtgcttg aagtatctta acaagacatt
     9541 gcatactgct gcagtcgcaa gacctgcgac gattccttca ggtataccac ttgtaaaaat
     9601 aaccgctaga atagctgaat aaacagcatt taaatttgtt ccgatgactt tggcataatc
     9661 ttgctggaat aaaaagtaga tcatattcat cactaaaatc gtattggtca atgaaccgga
     9721 aagtccagct aagaataaag aaacggaatc tgctttttgt ttcattagtt tgcgcagacc
     9781 tttataaacg aaataaggaa ccaccccaat caagatgcga ggaataaacg cgatcagcaa
     9841 ggctttccag cttccatgtt ctgttccgat gactgggata aacggagaaa agacaaaaga
     9901 caaaggtgtt tggataactg tgctacggat catgctgatc agtccaaatg taccgccaag
     9961 aaaagcacct agtcttggtc ctaaaacgat cgaagcaatg attacaggaa tatgcatggt
    10021 tgttgcattg attggaccga tagggataaa acctaaaggc gtaacagcta ataaaatcaa
    10081 aatagctaaa aacatcgcag ttaatgtaaa gttttttgta tttttcataa gtactccttt
    10141 tttctaagca tttctttcat taagagtcag catgacctgt tcgatgatcg tctgaatatc
    10201 agctaaggca ccttttccaa aatctccaca agctaaaaga gattctcttg gttcaatttc
    10261 ctgatagccg acagatctca atgtttccag attacgctga gtgatcgggt tttgatacat
    10321 attcgtgttc atggcagggg caatcaattt tggtgttccc actggaagcg ccattgcgac
    10381 tgttgaaaga agatcgtctg caatgccgtt tgctagcttg ccgataatgt ttgctgtagc
    10441 aggcgcgatg agaaacaagt cagcttgttt tgctagttcg atatgattga ttacctgcgg
    10501 atttgattct tgcatgacgt ctgtatggac tgcgcgcttc gacagagatt gcaaggttaa
    10561 tggcgtgata aaatgtgtgc tgcttttaga cataatcaca tctacctggg cacctaattt
    10621 gacgaattga cttgtaagat cagcgctttt gtaagcagaa atacttccag taacgcctag
    10681 taagatcttt ttcattttga ttcctccagt ctaattttct cagtaatcaa tgcagcaatc
    10741 tcagatttac tttgagcttc ttccactgtt ccatctttag ataagagata gccgtggtgt
    10801 gtctcgccat ggatttccgt caaatcattt gcgaaaataa aatcagtttt gttatttatc
    10861 agactatttt ttgcgactgc cagaagagat tcctttggta catctactaa tagtttgaaa
    10921 ccaatgattt ttgcttttgg ctgccattca cgtagataag aaatgatttt tggtgttttc
    10981 ttcaaaacga gcacaagatg ctcggtgtta gaagagatct ttgaaacagt ggtatctgtc
    11041 tgttcatttt ccgcaaacca attgtccagt tcttcttgcg ttgaagaagt gggtaggttt
    11101 tttgccagct gttcttctga aaaactaaaa gcaggagtaa aatcactgac tgccatactg
    11161 tggataatcg catcgtagtg ttcttttttc gttagttctt ctaattgtaa aaataagtcc
    11221 agtgcagatt cgatcgtata aatcgttata ttggagtgtc ttttcggttt cagtgcttgt
    11281 ttcgtagtca cgtaatcgat tgtggtatcc ggattggcag ctaaataatc agctaatgct
    11341 tgccctaatc gtcctgtcga atgatttgta atagcacgta cttgatcgat tttttcagtc
    11401 gttcctccag ctgtgattag aatcttcatt tatggtcacc tacatttact agtgttcttc
    11461 cttggtgtgt tccagcaagt agtttagatg ctgtttcagg caattgatcc agagtaatct
    11521 cttggacgac cagttcatct gcaatctgca aatccgccat ctgttgccat aaagaaagac
    11581 gttgatctat tggtacattg acagaatcaa ttccaatcaa ctggatgttt cgcagaataa
    11641 aaggcaagac agtcgcgttt agtttgattc ccccagcatt gccacataaa gctactgcac
    11701 cgttataaga gatcaatgga agaagactgc ttagctgctc gccacctact gtgtcgatga
    11761 catagtcgat ttgctgtttt cctaaagctt ttgttgtctc tggcagaaat tcatcaggtc
    11821 gtacgacttg caaagcacct agacttttta gccatgtgac agactcttct tttctggaaa
    11881 aggcaatgat tgactggaat cctagtttat gtaacagggc tatcgctgta ctgcctacac
    11941 cgccagaagc accagtaacg acaacttttt tatcggaggt cataccttga cggattaaag
    12001 cgttcacaca caacatagca gtaaaccctg ctgtaccaag gatcatcgct tgacgcagcg
    12061 tcatattttt tggtagagga actaaccagt ctcctggtac tttttgatat tggctataac
    12121 cgcctggatg ggtgacgcca agcccaaaac cagtagcaag tacaagatct tcttttgaaa
    12181 aacgtggatt gttactttgg acgacagttc cggataaatc aatccctgga gtcatcggat
    12241 atgttcgtat cacctgacca gactcgctca ttgccaatgc atctttataa ttgacatcag
    12301 aataggcaat ttttaccaag acttcatttt cagccaactc agtaaatggc agatcactga
    12361 ttgctgtatg gaaaacaggt tcctttatta cctgaaaaga tcgaaaagcg ttcatttgtg
    12421 agacactcct tttaaaattt tcttttatca taccataaag cctactcttt tattcttgtt
    12481 cttccgtaaa tttagacaaa aattaggtga aaaataaaga atcaaacaac tagaatttat
    12541 tccaaaagat aaagaaatat tcagcaaaaa aaattttaca aatggttgcc tttttcaata
    12601 aatttcgtat actaatacga gtggtagtaa acaggaagtt tactattaga aaacttcgat
    12661 tcggaggaaa ttatggaaat tggtgagaag ttaagaaatt tgcgcatcca aaagaatttg
    12721 acacaagaag aactaggcga acgcacagat ttgtcaaaag ggtatatttc ccagttggaa
    12781 cgagatttaa gttcaccatc aatggaaaca ttcttttcta ttcttgaagt cttaggtgtg
    12841 actccagaag aattttttca tcaagaagca gcgaatctgc aagtggttta ttcaaaagaa
    12901 gatcatacgg tttatttcga tgaagaaaac gggtatgaat tggaatggct cgtggctgat
    12961 tcgaatgaaa aagaaatgga gcctgtcttg ctgacttttg aaggtgcagg agaatataag
    13021 acgtttgaac cttcattatc tgaaacgttt atttttgtat tagaagggga attagagtta
    13081 agcttaggag agacggtcta tgtggcaaaa gcaggacagt cgatctatta tcaagcgaca
    13141 aaacaacatc aattaaggaa tcatctcaaa aaacgaacaa aagtattgat tgttgcaacg
    13201 caatcatatt tgtaatatag ggggtaaaaa cgtggggaaa ccaatcattt catttgatca
    13261 agtagtgaaa cgctatgatg atgaaacagt attaaaaaaa gtaagttttg agatcgaaca
    13321 aggaaaattt tatacactgc ttggtccttc cggctgtggt aaaacaacga tcctacgtat
    13381 tatagctgga ttcaccgaag ctacagaagg ggacatctat tttgaaggga aacggttgaa
    13441 tgatttgcca gctaataagc gacaagtaaa tacagtcttc caagattatg cactttttcc
    13501 tcatatgaat gtttttgata acgttgcttt tggattgaag atcaagaaaa tgtccaaatc
    13561 agatatcgaa aagaaggtaa aagatgcttt gcgtatggtc cagcttcctg gatacgaaaa
    13621 acgggagatt agtgaaatgt ctggtggtca aagacagcgt gtagcgattg cacgagcaat
    13681 tgtaaatgaa ccgaaagtat tgctattaga tgaaccgtta tcggcgcttg atttgaaact
    13741 acgtacagat atgcagtatg aactgcggga tctgcaacaa cgattaggaa tcacgtttat
    13801 ttttgttact catgaccaag aagaagcgtt ggcaatgagt gatgagattt ttgtcatgaa
    13861 taaagggaaa atcgtccaaa gtggtacacc cgtagatatt tatgatgagc caatcaatca
    13921 tttcgttgct gattttgttg gggagagcaa tatcgtggac ggtgtaatga tcgaagataa
    13981 tctagttgag tttgttggta agcaatttga atgtgtcgat ggcgggatgc gtctaaatga
    14041 accagttgaa gttgttttac gcccagagga tttgacgatc acgacaccag ataaaggaaa
    14101 actagtcgta acggtcgata ctcagctgtt tcgtggtgtc cattatgaaa tcatctgtta
    14161 tgatgaacag caaaatgaat ggatggtcca ttcaacgaaa aaagctaaag aaggaagcaa
    14221 agtaggattg gcctttgagc cagaagatat ccatgtcatg cgtttcaacg agtctgaaga
    14281 agaattcgat gcacgtcttg acagctacga agaatagggg ggacaagaat gaaaacaatg
    14341 cgcaggatct attccgtccc ttatattttg tggctcggtt tattcgtgat cgcgcctgta
    14401 ttaatgatca tttatcaatc tttttttgat atgaatgggc aattcacgct gaataattat
    14461 ctatcttatt ttacgtcggg gacatcgctt tctatgacga tcaattctgt atggtatgca
    14521 tttttgatca cgtttttcac gctgatcatc agttacccga ctgcttattt tttaacaaaa
    14581 ctgaagcaca agcaattatg gctgatgctg attattttgc ctacatgggt gaatctgctg
    14641 cttaaagctt acgcatttat tgggattttc agtgtgcacg gcagcgtgaa tcatttcttt
    14701 gattttatcg ggatcgggcc gaaacagata ttattcacag acttcagttt tctgttcgtg
    14761 gcaacgtata tcgagattcc atttatgatt atgccaattt tcaatgcatt ggaagagatg
    14821 aatccttctt tgatcagtgc tagtagagat ttaggcgcga atagctttga gaccttccga
    14881 cgagtgattt ttccgttatc attaaatggt gtgaaaagtg gtgtccaagc agtatttatt
    14941 ccttctcttt ctttattcat gctgactcgt ctgattggcg gaaatcgggt gatcacttta
    15001 ggaacggcta tcgaacagca tttcttggtt acacaaaact gggggatggg ttctacaatc
    15061 ggtgtgattt taatcattgc tatgttcatc gtgatgctcc tgacaggcga gaagaaaaaa
    15121 ggaggtcgct cataaatgaa aaaatttaaa tggtcgtatc tttatttagg gatcgtcttt
    15181 ttattcttat atgcaccgat cttctatctg attttttatt ccttcaatga aggaggaacg
    15241 atgaacagct ttacaggatt tacttgggaa aattatgaag ctgtttttga agatacacgt
    15301 ttgatcatta tcgtattgaa tacgttcatg ctggctttct tatctgcatt gatcgccacg
    15361 atcatcggga catttggggc tatgggaatc tattacacga aaaaacgacg tgcacggaca
    15421 gcgttgttga gcatgaataa catcttatta gtctctcctg atgttattat cggtgctagt
    15481 ttcttgattt tctttacact actaggattc ggattaggtt ttcagagtgt attgctttcc
    15541 catattgctt tcagcattcc aatcgtcgtc ttgatggtct tgccaaaatt gcaggaaatg
    15601 aacgattcaa tggtcgatgc ggctagagac cttggtgcta ataatttcca agtagtcaaa
    15661 aatatcattt tgccttatct ttctccagga atcatagctg gatattttat ggcatttacc
    15721 tattctcttg atgattttgc ggttacattt ttcgtcacag gaaatggctt tacgacgctc
    15781 tcagtagaga tctattctcg tgcaagacaa ggaatcagtt tagaaatcaa tgcgctaagt
    15841 gcgttagttt tcttgttctc catgatattg gtcattggat actactttat tagtaaagaa
    15901 aaccgtccaa aacggctgaa gaaaaacaga agagtacgcg agggggtggc acggctgcga
    15961 tgaaaaaatt acaatctctg tttttaggta ttctcatcat tattgcggtg ctgctgcttg
    16021 gtgttcgtca acttgaaaaa acaagtggca tgagtggagc aaaggtactt accatctata
    16081 actggggaga ttatatcgat cctagtttga tcacgaaatt tgaaaaagaa tatggataca
    16141 aagtgaatta tgaaacattt gattctaatg aagcgatgtt cacaaaaatc cagcaaggtg
    16201 ggacaaacta cgacatcgct ataccaagtg aatatatgat ccaaaaaatg atcaaagaaa
    16261 aactagtact tccattagat cattcgaaga tcaaaggatt aaataatatt gatccacgat
    16321 ttttagactt ggattttgat ccagataatc agtattcgat tccatatttt tggggaactt
    16381 tagggatcgt atataacgat aaattctttg atgcaaatca aattaagcat tgggatgatt
    16441 tgtggaagcc ggaattaaaa gacagtttga tgctgattga cggtgcacga gaagttatgg
    16501 ggctttcttt gaatagttta ggctattctt tgaatagtaa gaatatggca cagctgacag
    16561 aagcagcaaa taaattgaat catttaacac caaatgtcaa agcaatcgtg gcagatgaga
    16621 tcaaaatgta tatgatcaat gaagaagcct ccgtggcagt gacattttct ggcgaagctg
    16681 ccgatatgat gtgggaaaat gaacatttac attatgtgat cccaccagaa ggatccaatc
    16741 tttggtttga taatatcgtt atgccaaaaa ccgtgaaaaa taaagaaggt gcctatgatt
    16801 tcatcaactt tatgctagaa ccggaaaatg cggcacaaaa tgcagagtat attggttatt
    16861 ctacaccaaa tgaagaagca aaagcgttat tgccaaaaga catttcgtcg gacgaacaat
    16921 tctatccatc tgatgacacg atctcccatc ttgaagtcta caaagatcta ggatcgaaat
    16981 acctaggcat ctataatgac ctattcttag aattcaagat gtataggaga taatggcaaa
    17041 actcagtaag aatggggact tatcaacgtt ctaaagagct aaaaatcgtc ctttttctat
    17101 ctgagttttc caatcttcta ccaaaagcgg atgaaacgcc gctttttttg tgttatttta
    17161 ttttttctac cacgtttttc taccagagaa aagtggacaa aaccattgta aaatagggcc
    17221 cgagacgggc tagtaaatct aagattgaaa aagttacaga aggataaagt aattggaaac
    17281 tatgcaaata tacttaaccc ttctgtaatc tattaataaa actaatatat gaatccagtt
    17341 caatatttgt agctatgaaa aaacataaaa atcatattag ataaaaacta aattggtaga
    17401 taaattattt tgtcagatat ctgaggcaaa tattcgacta tttgtgtttc aaagtaaaaa
    17461 ttggaataca aatatagaat agacatattg cagccgatag tttattatgc ttttagacac
    17521 attaaactgg aggaaaaaat gaaaaagagc gctaaagatc gagtttttga tgagctgata
    17581 cgactaacag ggaatatgtc ttctgtcact gccgagaaaa tttcgaagaa tctaggtatc
    17641 tctcgtcaga atgccagcca ttatttgacg agattagtgg aagataaaaa ggtcgagaaa
    17701 ttaccgggaa agcctgtttt gtggaagcct ttagatgaat atgcggttgt agataatacg
    17761 gagcaaataa acgaagcctt tcattctgta gttggtcatg acggaagtct gagagaggtc
    17821 attcaaaaat gtatagccgc tgtcaaatat ccgcctaacg gactcagtgt attaataaat
    17881 ggagccacgg gtgttggaaa aagtttttta gccacaaaaa tttttgaata cgctatccat
    17941 gagcaaatta ttgaaaaaga tgcaccattt gctatcttaa actgtgccga ttatgcggac
    18001 aatcccgaac tgctttcggc aacactgttt ggatacaaga agggggcctt tacgggggct
    18061 gaaaaggata ccgatggttt attggcaacg gccaacaacg gctatctttt tctcgatgag
    18121 gtccatcgcc tatcgaaaga aaatcaagaa aagctctttc tatttattga tactggaaac
    18181 tatcgtccag tcggtgaaaa tgtaaattgg cattctgcta aggttcgttt tattttcgcc
    18241 acgacggaaa aaggtgaaaa ttatttatta gacacctttg atcggcgtat tcaaattagt
    18301 gttatgttgc caacttttga agatcgcccc attcgagaac gattagagct gattcaatta
    18361 ttttttcaaa atgaagcgga tgttttacaa aaagatatta ttgtttcaag agaagccttg
    18421 tctttaatgt tatctcatcc ctttagtggg aatattggta agctgaagaa tatcattaaa
    18481 atcagttgtg cggatgtgta tagtcgaaca caagaagaac cattaacgat tgggaaaaat
    18541 gagatcttga ttcagttaaa tatgctggaa tccgaagtag aaagcttgcc gattgctagt
    18601 ttattgataa agaaaaatca aaagataaaa acggatcgaa tcaaaccgtt tgattatgat
    18661 tcgattttgg aaaaaattac tcagctgata aatgaaggta atttcaatga aaatttttct
    18721 tcgataaaga atgaagtaca gcatttggtg catcacattg ataaggattc ttttttatca
    18781 atgagtagaa aattgatgat tcagatgttt agaaaggtct gggtatccgt tttaggaaaa
    18841 acgtacggat tgacgacgag tggacctgtt gcagaaatta ttagcagatc ctatgtcatt
    18901 tccccaagct ataagacaga cttaaccggg ataaatcgta cattagctgc aaatctacct
    18961 cggacgactt acttatgtaa tcagttcttg gagcatttac caccgctctc agaaaaggac
    19021 gagaactttc taagactgct tattgtcgtg actttatctg attatgtaga tgaatccatc
    19081 gagctgaagg gcctgttgct tgctcacgga gatagtacgg ctagcagcat tcaagcagta
    19141 gtgaatcaat tatgcggcaa ttatgttttt gaagcgttgg atatgccagt agagagtaat
    19201 gtgtcagaga ttgtcgaatc gacaaggaat ttcgtgaaac ggcaagtgaa tacagatagt
    19261 ctagtgctga tcgtagatat gggctcttta aatcaaattt acacacagat caagaacgat
    19321 ttaaaagggg agttacttct tctgaataat ttgacgactt ccattgcact tgatattggc
    19381 ttgaagatga cgatgaatgc gccatttaaa cagattgcgg aggatgcagc agacaagtat
    19441 tcaatcaatg ttcaatactt tgaaggcttt tctcaaagta gcaacattat catttcctgt
    19501 atgtcgggtt tggggatttc ggacaaattg aaagatgttt ttcgtcatca tttatcggat
    19561 gaatccatcg aagttttcac gaaggactac agagaactac gtgaattaat cgaccaaaat
    19621 gatgaaactt attttgacaa gacaaaattg gtcatcacga cttcggatct tccacgttcc
    19681 ttttcaattc ctaatattaa tgtttatgac atcttagacg gtgatggagc agccaatttg
    19741 gagcatattt tgagtcctga aatgaagccg gcagaattta accgcttgat tcaggagcta
    19801 gtaaaattct tttcgttgga aggaattgcg gaccgtttga actttctgaa cccaacgatc
    19861 gtgatgagtg aagtggaaac agtcatttct aaatatgaaa attactatca tttcacttta
    19921 aatggccgtg taaaactaaa tctctatatg catacggcat tgatgatgga acgtttattc
    19981 ttggctcgga cagaaaaatc tgaagaaccg aattatgatt tgaaaccaga tgaagatgaa
    20041 ttttattatg tttctagaag tatttttcaa tcaatggaga tgaaatataa ttttaaagtc
    20101 aatgcttatg agctttcgct gctctatgaa ttattaggac cgtatattca aaaataaagt
    20161 gtctaaaaaa gt
//
LOCUS       DACDIE010000047        20083 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.047, whole genome shotgun sequence.
ACCESSION   DACDIE010000047 DACDIE010000000
VERSION     DACDIE010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20083
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.047"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            72..506
                     /locus_tag="IV250_001943"
     CDS             72..506
                     /locus_tag="IV250_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8769207.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(584..1117)
                     /locus_tag="IV250_001944"
     CDS             complement(584..1117)
                     /locus_tag="IV250_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HAP8769208.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(1211..2389)
                     /gene="mutY"
                     /locus_tag="IV250_001945"
     CDS             complement(1211..2389)
                     /gene="mutY"
                     /locus_tag="IV250_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAP8769209.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(2382..3179)
                     /gene="recX"
                     /locus_tag="IV250_001946"
     CDS             complement(2382..3179)
                     /gene="recX"
                     /locus_tag="IV250_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAP8769210.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            3286..4668
                     /gene="rlmD"
                     /locus_tag="IV250_001947"
     CDS             3286..4668
                     /gene="rlmD"
                     /locus_tag="IV250_001947"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP8769211.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(4842..5420)
                     /locus_tag="IV250_001948"
     CDS             complement(4842..5420)
                     /locus_tag="IV250_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAP8769212.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            5742..6212
                     /locus_tag="IV250_001949"
     CDS             5742..6212
                     /locus_tag="IV250_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAP8769213.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(6320..7546)
                     /locus_tag="IV250_001950"
     CDS             complement(6320..7546)
                     /locus_tag="IV250_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAP8769214.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(8300..8500)
                     /locus_tag="IV250_001951"
     CDS             complement(8300..8500)
                     /locus_tag="IV250_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP8769215.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(8634..9242)
                     /locus_tag="IV250_001952"
     CDS             complement(8634..9242)
                     /locus_tag="IV250_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAP8769216.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(9327..9797)
                     /locus_tag="IV250_001953"
     CDS             complement(9327..9797)
                     /locus_tag="IV250_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAP8769217.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(9845..10759)
                     /locus_tag="IV250_001954"
     CDS             complement(9845..10759)
                     /locus_tag="IV250_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP8769218.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            10905..11708
                     /locus_tag="IV250_001955"
     CDS             10905..11708
                     /locus_tag="IV250_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAP8769219.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            11886..12833
                     /locus_tag="IV250_001956"
     CDS             11886..12833
                     /locus_tag="IV250_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP8769220.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(12901..13194)
                     /locus_tag="IV250_001957"
     CDS             complement(12901..13194)
                     /locus_tag="IV250_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HAP8769221.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(13222..13887)
                     /locus_tag="IV250_001958"
     CDS             complement(13222..13887)
                     /locus_tag="IV250_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAP8769222.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(14027..14659)
                     /locus_tag="IV250_001959"
     CDS             complement(14027..14659)
                     /locus_tag="IV250_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8769223.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(14666..15733)
                     /locus_tag="IV250_001960"
     CDS             complement(14666..15733)
                     /locus_tag="IV250_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP8769224.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(15730..16461)
                     /locus_tag="IV250_001961"
     CDS             complement(15730..16461)
                     /locus_tag="IV250_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAP8769225.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(16630..17109)
                     /gene="greA"
                     /locus_tag="IV250_001962"
     CDS             complement(16630..17109)
                     /gene="greA"
                     /locus_tag="IV250_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAP8769226.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(17245..18420)
                     /gene="mltG"
                     /locus_tag="IV250_001963"
     CDS             complement(17245..18420)
                     /gene="mltG"
                     /locus_tag="IV250_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAP8769227.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            18624..19793
                     /locus_tag="IV250_001964"
     CDS             18624..19793
                     /locus_tag="IV250_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP8769228.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
BASE COUNT         6072 a         4019 c         3229 g         6763 t
ORIGIN      
        1 tttgaaaact tgccatactt tttatttgtt ttattttcca gtatactata cgaaaggaag
       61 gtggctagac catgaaagtt gtacaaacaa aagatacaat gagcaatatt tacttagatg
      121 ctgtacggat caggcatcaa gtttttgtag tcgaacaggg tgtgcctttg tctcgagaaa
      181 tagacaaaga tgaagcccat tgtatccact tcgtcctgta ctcagataaa aaagaaccgc
      241 aaggaactgt acgtcttttg cctttagaaa acggaaaaat gaaattacag cggatggcga
      301 ttctttctga atacagacac caagggttag gaaaaatatt gattgaagaa gcagaaaact
      361 ttgctaagaa tcaaggctat aatacgattt tattaggggc acagtccaca gctgaaactt
      421 tttacgaaaa actaggatat acagcttacg gcgatccttt tgaagatgct ggcatgcctc
      481 atatttatat gaaaaagact ttataaaaat ttatttcgaa aaaagcaaaa aacagcaagc
      541 ttttgagaaa aatctctcac tggcttactg ttcttttttg tgtttactta cgcgacaatt
      601 gcttgtaacg gttataccaa atatcaatat agccttccga gaacgggcca tctccattat
      661 tgatccagtc aaccagaatc ttcacatttt cttttaggat gaagtcaata tcgttcgggt
      721 aattcatcat tttgctgtgc atctcatatt catcgacatc caatagtcgt ttctcgccgt
      781 caggaaatac cttgatatcc aagtcatagt cgatatattt caaggcctca tcgtccagca
      841 aaaaaggtga agctagatta caataatagg aaaccccctt ttctctgatc attgctatta
      901 cattgaacca gtatttttta tgaaaataaa caatcgctgg ttcgcgcgtt acccatcggc
      961 gtccatcaga ttcagtgact aatgtgtgat cattcacgcc gatcatagag tgttcgcttg
     1021 tttttaatac catggtatct cgccacgttc gatgcaattt gccgtcgtgc ttgtaacttt
     1081 ggatcgtcac aaactctcct tcttttggaa ctcccatagc aacccttcct taagcagcat
     1141 tttgtgcttt gtgtttgggt gttcattaca ccgacatcat aattattata tcaataaaaa
     1201 tgagctttgc ctagaaagga ttcctattgt ttcgattttt ctctaactga tctaccagtt
     1261 tcatttggac cttaggaaaa acgaccgaat caaatgcatc aggcttcaac cacttcgaag
     1321 ttttgttctc ggtaaattct tgttctgctc cctcggttgc ccgtccgtaa aaaaggagaa
     1381 catgccattt cagatgacta aaaatatgag taacttctcc taaatgtcgt gtctgccaaa
     1441 caaagagttc ctgcttttca aatgggagat tttgctcttc ttctgctact aggtcatcaa
     1501 acagatcgat ttctggcttt gtttcccatt cttttttcaa tcgctcatac tcctcttgtg
     1561 tcacttccat cattggaaat gtccacatat tcgccagcaa tttttggcta tctctttctt
     1621 caaaataata cgctccagaa tgattttgaa gagctgcact gatataataa acgtcttttg
     1681 gctttgcttt ttttgttttt acaggaaaac tcgtttggat tcctcgtttg ttagctaaac
     1741 aaaaggcttg gatcggacaa gtctcacact ttggagaagt tggtgtacag atagctgaac
     1801 ctagatccat cattgcttgg ttgaattcac ctggatgctt ttcatcaatg attttccgca
     1861 tcgcttcatc aaagattttc ctgctagaag cttttgcgat gtctgcttca atgcaaaaca
     1921 atctgctgac tactcgcatc acgttgccgt caactgcagg ttctggaagt ccaaatgcga
     1981 tacttgcaat cgcgcctgtc gtatatggtc ctattccctt caatgagcta atttcttcag
     2041 gcgtttgcgg catttcccca tcaaattctg acatgatctg tttagcagca gcttggatgt
     2101 tccgggctct tgagtaataa ccaaggccct cccatgcttt taaaagcttt tcttctggag
     2161 catttgctag ctcttcaatt gtaggaaacc attccatgaa acgataaaaa taatcgatca
     2221 ctgtgtctac tctggtttgc tgaagcatga tctcagagat ccagatacga taaggatcac
     2281 gattatagcg ccaaggcagg tttctttttt cttgttcata ccattgaatg aactgatctt
     2341 gaaattcttt tgtttcttta tctgtccatt tttcccattc tttattcact ttcctcttcc
     2401 ttttccgtaa tgaatcgctg gatcatatcc aaatcaaatc ctttttgata taagctttgc
     2461 tttactttta gatttctttt tttaggttca aaccttaaat ttcttcgcca tagtttatct
     2521 ccttgtttga ctaaataatc atattctatt tcttgatctg cttcttgcgg aagatcagct
     2581 atcgcttgtt ggatcacttc ttgagaaaag cctttcgtca tcaatgtttg ttggattttt
     2641 ctcaataatt ctttttggct ttttccgtga ttttttccaa acgctttttc ggctgtctga
     2701 tgtgcatttt gaacttggtc ttcaaatgta tattggctga gcgcttgttc aatgatttca
     2761 ggtttcaacc ctttttgctg catctgctgc gctaatttct ttggtccttt gtcacttaag
     2821 cgcatattcg tgcgcacata actttcccca tagaccaaat catcgacaag gtccaattct
     2881 tttaagcggg ctacgatttt atttcgatct tcttgaagga tttccttttc ttttaagtat
     2941 gttcggattt ctttttccga tcgcaactga taacttaagt aattcattgc taggtgaaaa
     3001 ccgaaatcag ctccgctact cttcttgatc tcttggatag tttcttcgtc taattcccgt
     3061 ccttttaata atcgatagcg tactaataca tcttcagata cttgtaaagc ttctccttca
     3121 gataaatcta cgcgatagaa agggcctttg cctttactga ttctcgtaac ggttaacata
     3181 aacatcctcc tcgaacatat attctctatc ataccacaga aaatccccgt aatcaggtat
     3241 tatatgttag aataaaccac agaaaaaaga aaaagggaga tacttatggc agaaacaatt
     3301 gtcaaaactg gacaaacggt tcctttgaaa atcaagcgtt taggcataaa tggtgaagga
     3361 atcggttatt tcaaacggtt gatcatattt gttccatatg cactaccaaa agaagaagta
     3421 ctagtgaaaa tcacgaaagc aactcctcgc tatgcagaag gagaattaat aaaagtcaaa
     3481 aaaacaagca aagatcgcgt agtggcacca tgtcctgttt actatgaatg cggtggctgt
     3541 caattacaac acttagctta tcaagctcaa ttggatttta aaaaggatct gcttttgcaa
     3601 gcattggaaa aattcaaacc agcaggcttt cgatcttatc aacttttgcc gacgatcggg
     3661 atggatgagc cttggcatta tagaaacaaa gcacaatttc agttacgaaa aaataagcag
     3721 actcaaaaaa ttgaagctgg tttatacgaa gcaaactctc atcagttagt ccctataaca
     3781 gactgcttgg ttcaagaacc tcgaacacaa aaagtaatga atactgttgt tcaactattg
     3841 aataaatttg atgcacccat ctatgatgaa cggacaaaca gtggaatctt ccgcacaatc
     3901 atggttagaa tcggtgttaa aactggggag ttacaagttg tttttattac tcgcagtaaa
     3961 aaattcccgc aaaaaaacgc aatgatccgt gagatcaatg aacgactgcc cgaagttgtt
     4021 tcaatcatgc aaaatgtcca ggcacaaaaa acgtctctcg tgatggggga tgaaacgatc
     4081 cacttatggg gaaaagaagc cattgaagaa caaatcaatg aagtgacttt cgatctttct
     4141 cctcgtgcct tctttcaact caatcctcag cagaccgaat tgctttatgg cgaaggaatc
     4201 aaagcattag atgtccagcc taatgaaacg atcgtggatg cttattgcgg agttgggacg
     4261 atcggcttaa gtgtagcaaa acaagccaaa gaagtacggg gaatggatac cgttcctcaa
     4321 gcaatcgctg atgcaaaaaa aaatgcggaa agattaggat atcacaacac tcgatatgaa
     4381 gtcgggacag cagagcaagt tctgcctaaa tggttgaatg aaggttttca gcctgatggt
     4441 atcatcgtag atccaccacg tacgggtttg gataacgcat tgatccaatc attattgaaa
     4501 taccctccaa aaaaacttgt ttatatctcc tgtaatgttt ctacacttgc cagagatctg
     4561 gttaatttaa gtaaaaagtt caagattgaa tatttacagt ccgtcgacat gtttcctcag
     4621 actgcacgtt gtgaagttgt cgtgaagatg acgagactcg ctgactaaat gaaagaggtt
     4681 gtgaaaaagt ctgccctgta tcaagtaata agaacagcca aacaaaaata gctcttcatg
     4741 ttttatgaat actgtacttg tgaccgaaga gttaacagga ggacactatt tatttaaaaa
     4801 aagggttgtg acatagtttt tgtcacagcc ctttttgatt attattggat attcacgtct
     4861 atcacttgat aaaatgcatt tgtcgtatcg ctgatattcc atactgctaa aatcacatta
     4921 tatccttttc gatcctgcgg aagtgtgatc gtttggttga ctgtttttgg tggaacagtt
     4981 gctttgtcgt ctatttttgt gatcaattca aaatcactaa atttcaaagg ttgattagga
     5041 ttccaagttg gtttggtaat gtagtaatcc caagtacttg tccgatgttg tgctgttaaa
     5101 ttccaagtaa tatttaatgt tcctggatta accatgtttt tatgccatct gttagatgtc
     5161 tgttcatcta aaggctcaaa gccgctgact cctgcactag caatttttcc atcaatgaat
     5221 gtatttttgg tagcttcgat actttgcggt tcccattgtg ctcttccgac attttgattt
     5281 aagtttcctg ctgctgatgt tcctaggtat gcacggcttc ctggagaagt aatataacca
     5341 tgcgcaaaac ctggaatgct aaaaccgaac aaccctaaac taactaaccc aataaacaat
     5401 aacgtactaa tctttttcat gtagaaacct ccttcatttg ataaccacat tgtaaacgct
     5461 ttattgtatt aacacaaaca ttatgctttt ataattttat gttgaagtaa aaaaggtgaa
     5521 aaaagtaaga tgcagattat aacagaagta gacgagaaaa gtacaaatag aaaacatgag
     5581 aatcaaatca tatgatcttt ttcaaaaaca tcattcatat aaacaaatgt tcaacttttt
     5641 aataacaaca attatcaatt tttttcttac ttcacaaact aaatacaggt cttgctgtga
     5701 gccttcatgg taaactaata ttatagaaag gagttagtaa aatgaagatg ataaaagata
     5761 ttcgtttttg ggtttgtgtt ataggaattg tcatattagg ctttttatca gggttgctta
     5821 gcggaaatcc cggaaaatat tactattctt tgcaacttcc accatttgca ccacctagct
     5881 ggatatttgg gccgatgtgg accttactgt atatattgat gggtatttct ctttatttgt
     5941 tacttaatca caacaacaaa aaacagcgaa acaatcttgt cggtcttttt gtgattcagt
     6001 tcatttttaa ttttatatgg tctgctctat tttttaattt aagaaatatt ttcattgcag
     6061 caatcgatat tacactttta gttattttct tgtcagtgct tatgtatcag ttgtggctgc
     6121 atcatcgcct ggcaatgtgg ctcatgattc cttattattt atgggtacta ttcgcaactc
     6181 tattgaatta ttcaatttat tttttaaact aagcgctaaa aagagacgtc ctgtaattta
     6241 acacatagaa aaagtgccac ctaagttaga ttttttgtct aatttaggtg gcactcttta
     6301 ttaaccaaac tatttttttc tagtacccac ttgttgttcc atactcatta ttctggtcat
     6361 aaccataacc attttgttcc tgtccagcgt aaggatcata actttgagta tcttgacttg
     6421 gtgtatagta ctgacttgta tcttctgttt gctgataata ggtattcgga tcttggtact
     6481 gatcatatgt gttttctgaa taggaagaat aatcggtgta cccttcacta ttcgatccat
     6541 caccgtttgt cgaatcgcca tcttccgaat catcaaagaa catcattgaa gcattctcat
     6601 ttttcaaatt aggtaatgta tcgcttgttt ttatatttaa ttgtttcttc agttcatttt
     6661 ggatcgaaag aagatcattt tttcctaaaa tttgatagta gatatcattg atcatttgtc
     6721 cttcgccttg caactggtct tgtttgattg tttcaaacgc cggggtgtag ttcgtcgcta
     6781 catcaagcat gtcatcccaa tctaagtccg tcgtcacatt gttctcaact gcttttaaaa
     6841 tttttctata attgcttacg ccatctaaac ttaaaacttt attgacgatt ttcgtcacta
     6901 cttcccgctg acgacgttgt cgaccgatat cgccttctgg atcttcgtgt cgcattcgag
     6961 aataggctaa acctttttct ccattcaatg tttgttcacc tactggtaca tggacaccat
     7021 ccaaagtaaa gtcaatttta ttattcactt tgatgccacc gaccgcattg atcaaatctt
     7081 ccatgccatc caaattgatc gttacataat gatcaatagg aatatcgagc agttgctcaa
     7141 ttgaatccat ggccatttcg acaccaccaa atgcgtaggc atgatttaat ttatctaccg
     7201 ttccataacc tacgatattc gtataaatat ctcggtccaa actgatgatc gttgattttt
     7261 tttgtttcgg attaactgtt actaccatca tcgtatcaga acgtccttgt tccgttcttc
     7321 ctaatccacc ggtatctaaa cctaatagca aaaccgaaaa aggttctttg tcatcaatgc
     7381 ttactctctc agaacgtttg gaggatattc gattagagtt tttagaaatt ttgtttactg
     7441 tttggctcgc ttctcccata aatctgattc cgtatgctga taatccggca atcagcaaaa
     7501 cgaatacccc taacaaggtt aatagaactt tttgccatcg cttcataata cacctctgtt
     7561 tcacattttc taaaatattg tctcatataa ctgttcaatc agcaaattca ctttttaatc
     7621 ataagaaaac tttaaaaaac atttttgtgt aacgattatt attttttaaa atttctcaaa
     7681 attcttaact atcataccat accgtgaaaa agatcgaaag accgaataca cgcagttcgc
     7741 aatcgtttta tcttttttta tgtatttttt ccagaaaaag cgaacaacaa ggtattttcc
     7801 aaataatcgt gcagtaaaat gatcgcacat acctttccca tctttttaac agtctttgcc
     7861 taccgttcag attatcattc gatttcacga caatttcttc ctatgactgg tccttttgtt
     7921 aaacccatct tctattcgtt ttatttccag ctcactgcat ggctcttaaa atgactcgaa
     7981 ctagtgattg cattaccatc atcttttaat ggatagatat gctcctcttc aaagtaaaac
     8041 gattttcttt taaagtaata gaattgacat ggtaatgagt atgtatacta gtggtttcac
     8101 aaatggtctt ttattcaaca tccaaaaact gaaatttctt taaaatccat tactaacgaa
     8161 gaaaatgttc cttgcttgat aattagttag gacatgtcaa gtgaacaaat gcatttactt
     8221 agccaaaaaa tctaaaaact tcataaatag acaaaaagac acgactctct aagaagtcgt
     8281 gtctttattc aacaaattat tataatttag tcacttcagc agcttgaggt ccgcgtgcac
     8341 cttcaacaat cgtgaattct acttcttgac cttcttctag gctcttgaag ccgtctcctt
     8401 gaattgctga gaaatgaaca aatacgtcat ctccgccgtc tactgtaata aacccaaatc
     8461 ctttttcatt gttgaaccat ttaaccgttc cgtgttccat aaatgtatat cctccaaaaa
     8521 agttcttgca agtaaccttg caagaacatt atacccaatt tagaaaacgc tagcaagctt
     8581 tcgctctttt attttccaaa ttatttttaa gactgatatg acggaggaaa tggttatttt
     8641 gctgcttcga aacgtttgtt tacttcatcc caattaataa cattccagaa tgctgcaata
     8701 taatctggtc ggacattttt atatttcaaa taataagcat gttcccaaac gtccaaacct
     8761 agaattggtg tctttccttc catcaatgga gaatcttgat ttgcagtaga ggtaatagct
     8821 aattttccat tttccattac aagccaagcc catccagaac caaatcgtcc agcggctgct
     8881 tttttgaatt cttctttaaa agaagaaaaa tcaccaaaag cttcattaat cgcttctttt
     8941 atttctcctg taggttcgcc accagcattt ggtgccatga tttcccagaa aaatgaatgg
     9001 ttagcatgtc cgccaccatt gttacgtaca gctgtcttga tatctgttgg aatagcgtcc
     9061 atatcagata ataattcttc tattgttttt tcgcctaatt ctgggtattt ctcaattgct
     9121 gaatttaaat tcgtcacata agtattgtga tgcttatcat gatgcagatg catcgtttct
     9181 tcgtcaatat aaggttccaa tgcgtcatat gcataaggta gatctggtaa tgtgtaagtc
     9241 ataaaaaaat tcctcctcaa tgttttctta ataatgtcta cattacaaag agtaacaaag
     9301 agaaggaaat tcttcaattg ttatgcctag tcgtcttttt cactttgacc tacttcttct
     9361 tcaaaaacct cagtttcttt aaaaataaac aacttgctga aaatatagtt cgcaaggata
     9421 acaataattt gagtgatgat tttcgccaca tactcattgc tgttcataac attgatcaac
     9481 aataccattg cccccatgtc caatccgtaa gaaaggatac ggtagaagaa gaacttaccg
     9541 cattcaataa gtgcatgtgt aaaattcggt gattttgaat ggaataccca aagtttgttc
     9601 gttacaaaag caaacaatac agaacaaaac cacgaaatag aattggataa ggccatactt
     9661 aaatcaaaat attggcgtgc gagagcaaaa ctaacaaaat ttacaatcgt agccaaacca
     9721 ccaaaaaaga gatagactag tatctcccaa tatccttttg cttctaaata tccttttaac
     9781 tgtcgatact gcttcatgag ccaacctacc ttattaaaaa ttcttattta tcatactcta
     9841 tatttcaata actttcatct ttatttaaaa aaattaattt tttttgatcc tttgcctttg
     9901 tctctgcttg gatcgtcaca tgtgtaatac catattttaa ttttaataaa gaaattatac
     9961 gaggatatat cttttcaatc tcgcttagta gctgatcttc aaaatcaata tgtccgtcga
    10021 aaataattat cttttcgcta tactgccaaa tatggacatg atgaacatcc tttaccccat
    10081 cgattgcttc aatttcttgt tccatttttt catagtcgat agcaggtgca ctttgtatca
    10141 aaatagcggc agcttttttg atcaccatta tcgcttctat caatatatat atcgaaatga
    10201 tcagtgtaat aactggatcg atccagtaga tatcgaaaaa acggatcaaa attgctccga
    10261 taatcacact taccgaagac agtgtatcac ttaacaaatg caaataacta gattttatat
    10321 tcaaactttc atctgcttct tgtcgtaata ataatactga gaacaaattc gcgagcaatc
    10381 caataactgc tactgttagc atcaaatgac tatttatctt ttctggtgaa gtgaaccgct
    10441 tgaatgcttc aacggttaaa aacaaggata atatgatcaa tatgaatgag ttgatgaaag
    10501 cactcaatat ttctaaacgc tggtagccat acgtacgttt tttatttgct tcttttcggg
    10561 ccattttttg tgccccgtaa gaaaaaataa ttgccaatgt atcactaagg ttatggaaag
    10621 catctgaaac aagagacaaa ctccctgaca taatccctcc aataaattct gataaagtga
    10681 tcaccgcatt caaaaccact actccaaata gtttttttga tgatctctgt ggctgtgtag
    10741 gcactattcc ttcctccatt ttatttatat tggatagtct ttagacaaga ataacatttt
    10801 tttgatattt caccaatcat tcagctgttt ccttaaagca cttgtatttc acttgatact
    10861 tcgctagaat tgcaagtgat gacactacta gatgaggagt acttatgaca acaaaacaat
    10921 taattatcag ttcctttata cgattcaaag acctaaagga ggcgagaaat gtcccatttt
    10981 ggaaagccat tgtctatctt ttagttttga gtataattat ggctttgcca atctcttttc
    11041 aagtttttca agtcttaaac aacattcaat tggatgggca aaagattgct actcgcattc
    11101 ctgattttac aatccaaaac gggcagcttc aaaccgaaga aaaagaagga ttcatttacc
    11161 aaacaaactc cattatcttc acatttgatc cagagggaaa acgaacagaa caggacatct
    11221 ctactgattt gatggggaat tttgttagtg tcgggatgtt aaaaaacaaa cttatcatag
    11281 ctcttcctaa tacaggcacg acttctgctt tgttgaacaa taaccaatta gaacttccct
    11341 acaccaatga atctttaaaa aacttaacag gtaagcagct ccgttctttt ttaagtgaag
    11401 catctatacc actttggata aaagtcctga catttatatt ttctatttat cctagttttc
    11461 tgaatctgct gatcactttg ctatttacca acattgctgc ttttctctat gcacgctttc
    11521 gactaacaaa agctactttt cttgattgtt taaaaacatt gatctattca atttcattgc
    11581 ctactataat tgcaacaatc ttgatggtct ttcttccttc ttttgacact agcgccttca
    11641 ttgcttttgc aggaatcttt atttttgctc aagctgtcaa aggatgggca aaaatatcga
    11701 tcaattaaga aataaagaca actcgttatt tctacttcaa tagacccttt tagaggttgt
    11761 gacatttttt tgtcacagcc ccttttttat ttgaaaaata tttctttctc aaaaaacaag
    11821 caaggcgctt caaagttctt cttgtatgcg ctacacttag agtaaagaaa ggcgggaaat
    11881 taactatgtc agaaagaatc atttatgcag atcgtccatt tcgtgatgaa tttatcgaag
    11941 aaatccatac tatagcgcct ggttatatct ttaaaacaga attacaacca gaggatctac
    12001 aaaatgtaga gatcagtttg ggctggaact cagactacca agaaaaacta ttagcctctt
    12061 ctaccttgaa atgggtccaa tctatctcag ctggtgttga tcagctaccc cttgagactt
    12121 tttcaaagaa acatatttta ctttcaaatg gtagtggcat ccacagtgaa tcgattacgg
    12181 agcatgtctt aggaatcatc cttgggtatt ctcgtggtct ttttcaagca caaaaggcac
    12241 agagtgacat gaaatggtta ggttcatcgg tccattacca atctatccaa gggaaaaaaa
    12301 tactaatcat tggaactggc catattggaa aaatgattgc aaaaaaatgc aaagcattag
    12361 acatgaactg ctatggaatc aatacatccg gtcatccagc agaaggaatg gaaaagactt
    12421 tttctttaaa tcaactaaag gaagttcttc ctgaagtaga tttcgtagtc aatatcttac
    12481 ctctaacgga ggaaacaact gggctctttg atcagcatat gtttgaacac tttgatccaa
    12541 aatctgtatt catcaatgtt ggccgtggtg cttctgttaa gacacaagat ctggtgcagg
    12601 ctttaaataa ccagcaactt tcctttgcag cattagacgt ttttgaggaa gagcctcttc
    12661 cacaagatca tcctctttgg aaaatggaca atgtactgat tacttcccac attgcaggtt
    12721 tgacacctga ttttcaaaag aaattaatgg ctattttttt aaccaatctg aaaagctact
    12781 ttgccaccca tgagcttcaa accaatcaag taaaattgtc tgcaggatat taatagctga
    12841 aaacaatatt ttcaaaagaa aaaagagaag gtgacaaaaa ttgtgtcacc ctctcttttt
    12901 ttattgtttt ttaaagtgat aggttggctc ctccaggttg ggatcataat caacaagtaa
    12961 atcatatcct ttaaagaacc attcatctgc ttcctcgata aaaaaaagtg tcccatcaat
    13021 ttccactttc tttacaggat gttctggctg ttcaactgac attccaatcg aaaaaccatc
    13081 atgaacctct gttttgccgt agacttttcc aaaaaaacgt attccgtaag tttctggcag
    13141 atcaatttct gagtcaaacc acttttgtgc ttttggcgtt accgttaatt tcatcccaac
    13201 aacctacttt ctcttttctt tctattgatt catcttatcg tctagaatat ccacatcgcc
    13261 tgtctctcca ataaccagta gattatcatt ttcacgaaca tattcatctg ctgcaggaga
    13321 agcgataacc gtttgatcta ctcttcgtat ggcgactact gttaagtcaa aacgttgcct
    13381 gaaatttaat tcagctaatg ttttgttgaa aaatttagga ttggagactc ggatttctgc
    13441 taaagaatat ttatctgaca actctaggta atctaaaata ttctttgaca ctaaattatg
    13501 cgcaatcctt attcccatgt ctcgctcggg atggacgacg cgatcagctc cgatcttttc
    13561 aagtactctg gcatgatact cattttgggc ttttgctaaa atatttggta cgcccatttc
    13621 ctttaccatc agagtaacta agatactggc ttgaatatct tctccaatag cgacaattac
    13681 atgatcgaaa tttcttatgc ctaatgatcg aagtgtcatt tcatcctgtg catttgccac
    13741 aacagcatga gtggctatat tcatatattc attgacccga tcttcattac tatcaatagc
    13801 cagtacttct tgaccagctt cgatcagtgt tcggcagata cttccgccaa atcgtccaag
    13861 acctacaatt gcaaaatttt gtttcaccgt tttcctcctt tagtatcttc ttttctcagt
    13921 ataacaaagc ctttacaaaa acaaaagcaa gcatcattat tgtaaaaaag ccaagaaaaa
    13981 taatagttat tttctcagct tttgattatc ctctttatca tcgtttttat ttcaccaaac
    14041 catgtttgaa tgcatagata gcggcttgtg tacggtcttc cacttctagc ttagccaata
    14101 tattggaaac atgtgttttt actgttttca gtgtaataaa caattcgtcc gctatttcct
    14161 gattactttt tccttcagaa atcagcatta aaatttcatt ttcccgattc gttagttctt
    14221 catgtaatac aggctcattt cgccgactca ttttttccat catttttgtt gtcacttctg
    14281 gttctaatac tcgctcgcca cgttgcgttg cccttatagc atcagcaatt tcatgtgcgg
    14341 tagaggtctt caacaaatac cctgctgcac ctgcttcaat ggcaggataa actttttcgt
    14401 catcaataaa actggttacg ataatgattt tagcttctgg ccaatctttt aagattgctt
    14461 ttgttgattc gataccatcc atttcttcca tgactaagtc cattaaaatg acgtccggtc
    14521 gaagtgtcat cgccttctca tagccttgtc gtccattttc agcttctcca attacttcaa
    14581 tgtcttcttg aatagataaa taagaagaga cgcctaagcg caccatttca tggtcatcta
    14641 ctaataaaac ttttatcatc cttatctatg cctccttcat caaagggacc ttgatttcta
    14701 cacttgtacc ttgaccttta aaactaatga tttttactgt tccaccaata ccagctactc
    14761 gttctttgat attgttcaat ccataacttc ccgttttcgt ttcattcata ttaaaccctg
    14821 ttccgtcgtc aataatacgt aacaaaagat tattgtctat tttgtgcaaa tatacctcta
    14881 attcattggc tttagcatgt cttaatgtat ttgataacaa ttcttgaacg attcggaaca
    14941 agtgatcctc aatggaacta gatagtttta catcttctac atcccatttc agcgaaatct
    15001 ggattttatt ttgcaattcc ttcaataact gctcgatacc ttgctttaaa cttttctctt
    15061 ctaaattgac tggacgtaga tgaagaagca gcgcacgcat ctcagactgg gaagcgttga
    15121 tgatctctgc taccattttc aactgcttgc gaaacatttc tggtgtctcg cttttttctg
    15181 cttgctccgt caatgccgac atcatcatca ttgctgcaaa caattgttgc gaaactgaat
    15241 catgcagctc acgggctaat cgatgtcttt ctaattctaa aatttcttct ttggtttgtc
    15301 catctacata ttggggacgg ctcgttacaa tttgtaattc actagatact tcaatcattt
    15361 tttcttttat cttagtgatt tccttatcaa tatctttgat gtataaatcg tccgatgcac
    15421 ttgggatcgg aagaagcaac aatttggatt cataattgcc agaagaaagt gcacgcagct
    15481 tttcttcgat tttcccgtat tttgtctttt gaataagtga taataataga aagacaatta
    15541 atcctacacc taaggaaatg gctaacaaat ggaagataag cggaacatat aataaacgtg
    15601 cacgcagtaa ttctccccac caatgacttt gattgctggc ataaaaataa gtgaaaagtg
    15661 aaaatagaat aataaggaaa gccgcaatcc ccgaataaac agctagcatc gctctggata
    15721 tttttcccat catattcgta tcacctcgac atctccaatc aaggtattag taataatctt
    15781 caatctacga gggttttcat cataatcact gctgtagatt ttgatttgtt catttttcaa
    15841 agaaaattgc tcttcttcaa acaagacatt tcctactaat gtcgcatgct ctaatcggat
    15901 ggctacgccc aaaggtacta agatccgtgt tctgcctatc ccttttctta caatcacaat
    15961 attatcatct tttggcaaaa gcgtattgcc caaatctatg atagtatcac cagaaatgac
    16021 ggcgatgttg atatcgtccc attcatagac gtcgtttcca atacgttggt taccgaataa
    16081 ttgctgtctt ttccgctcat tattatgtgt ttttaattgc tctgtttgga tcatcatgat
    16141 ctgtttcttt cgccaaaaag catttttggt taaatctatc cctgaaatct ctacaccttt
    16201 cagaccaata aataaaacgg caaatacagc catcatccat aaagcaggac tgttgactaa
    16261 gctaaaaaaa atgaccaaac ttcctaatat caattgaaaa ttttgaaatt tagtacgagg
    16321 atgtttgcgc atagctagat atatattgaa aataccgaat atcactaaaa gcaaaagtcc
    16381 agtattgttt acgatttgcc aaacagcaaa taacagcagt agtgcttcaa ctaccacaaa
    16441 aaaacgccaa gaactattca tcgctctttc tccctcctga cacattctca tttcatcata
    16501 cctatttttt taactgttgg ctataggact ttagatgtaa gatcgtttgt tgaaatctaa
    16561 aatacacaaa gagaaaaaga tctagagtgt atcatcagta agatgaactc tctagatctt
    16621 tcagtcagac tacgcttgtt ctactttgac gattttaact aacatatcgc cacctggagt
    16681 agcaatagtt acttcatcat ttttacgttt gccaatcaat gcttgcgcaa taggagaatc
    16741 attggaaatt ttgccagaaa acggatctgc ttcagcgctt ccgacaatcg tgtactcttc
    16801 ttcttctcca tctggtagtt cgatgaatgt aactgtttta ccaattgata cctcatctgc
    16861 atctacaccg tcgttatcga tgatttgagc aaaacgaatc atattttcaa ttgttgtgat
    16921 tcttccttca acaaatgctt gttcatcttt cgctgattca tattcagagt tttcggaaag
    16981 atctccgaaa ccgcgggcaa ttttgattcg ttcaatgatt tctccgcgtt tgactgtttt
    17041 tagttcttct aattcttttt ctaatttttc tttaccttct agagtcattg gataaacttt
    17101 ctctaccatg tgtaacgtct ccttttttcg ataaaatacc gcaaaaggta atcttttgag
    17161 gaatatagtc caaaaaagat taccacgctt ttcttcaaat gtaaatgttt ttctaaaaat
    17221 gatcaatgtc tagagcctgt ttttctaaga attatttaca tatttttcca ctaattccat
    17281 atgttcttca taagttttag cgaaataaac atttccagtg ctcgtatctg ctacgaaata
    17341 ataatagtca ttttctgctg gttctaatac tgccttgatt gcctcttcgc ttggattgtc
    17401 aaaaggtcca ggtccatagc cggtattggt atacaaatta tatggtgaat ctacagctgt
    17461 atcttcatac gtcactaatt ctttgtgttc acctaatgcg tataaaacag agatatctga
    17521 ctgaagtggc atatttgctt tgattcggtt aaagaagact tgtgcaatgt ttttacgatc
    17581 attttctttt acgccttctt tttcaactag agaagctaga gttaaaactt cttgtacagt
    17641 caaatttttc tgcttgatag ctgaataata gttttgcatg accgtattcg tcttgttcac
    17701 catttcaatc actagatcct taagcgaagt actcgtgtaa taatcatatg tggctgggaa
    17761 taagtaacct tccaaacggt attttgtgtt ctcagcttta gatgcacttt ccaacagttc
    17821 tgggaatttt tggtgcaact cgttaaagaa agtctcatca ttcactaaat ctaaaaattc
    17881 tttcttgtct ttaccagtta ccttcgctac ccgttcagca atctgatcga tatcgtaccc
    17941 ttctggaatg gcaattttcc catctgcaac ttttgttggc tcagaagtac cgccttcttg
    18001 caactgtttg ccaatctcgt caagagtcat attaggcgct aactgataat agccagcttg
    18061 aaagccagtt aaatttttaa attttgtata ataattgaaa acaatcccgc ttttaataat
    18121 attgtccttc tccaaaatct caccaatctg cttgttagaa gagccactcg ggatctcgac
    18181 ttggactaat tggtcatctg atttatctaa aggcttcaac ccgctatcta cataacgata
    18241 cacggtaaat ccaagaaatc cgccaataat caataatgta agagcaatca ctaaaatgat
    18301 cttcctcaca atcttatctt cttttttacg cagatttctc cgcttgtcga ttatatctgc
    18361 tgtagctgcc ttactggctt cttttttatc tttacttggg tgttgtttgt ttcctgacaa
    18421 aacaactcct ccttactatc aagttcgtaa ctttattcat tatacatgat atttgaattg
    18481 gcgtgaatga ttttatttaa atattctatc aaaaatgtaa gtagtaaatc cattactata
    18541 agattaaaat aaatgttatc aagatacaag ctattttagc tttattcttt ctgtaaaaaa
    18601 gaaaggagac taaataaact attatgaaaa aaggaaaaca tgctaaaaaa agaaaatatt
    18661 catttttaat gatcgttgtc gtgttgttta gccttagtgc gttctctaca ggaactattc
    18721 ttggatggca attttacaat aatgaacact ctcagatagt tgcccatgct caatcagctc
    18781 aaaaaaagtt gatactgact accaatgaac aacagctcgt tgaaaaaatc gatcaactat
    18841 taagaaaaaa tgattatatt ggctcaattt acgtaaagaa aaataatcgc atcatcatgg
    18901 aaaaaggcta tggctatgcg aataaactca cgcaacaacc aaatgatcct tctctttatt
    18961 atcagattgg ttcggttcaa aaagcattga ctactctttt gattttaaaa caagtcgagc
    19021 gtgggcacct ttctttagat actaagctgg ctcaattcta tccacaaatt gctggcagcc
    19081 agcaaattac tatccgtgac ttgctgtata tgcgctcagg cttgcaccga actgcttctc
    19141 caaccatacc gatgactgat gaagaagtcg ttcaatttgc gattcagcac ttaaaattta
    19201 cagattatca gacctctcat tatgattcat tgaactttac catattgact ggaattttga
    19261 tcaaattaac aaatcaaccg tatgaaaaat tattgaccga tgatatcatc gaacctttag
    19321 gattacagca tacaaaattt tatgaccaag tcaaaaattc atccaatcat gcactatcct
    19381 atcaaatgtc taaagaggat gattacttta aaccactgac agaatctgaa acagagatcc
    19441 ataacgaatt agggactggt aatatcagta tgtccgtcta tgatttaaat acgctgttta
    19501 cacaagtttt atctggaaaa ctagtttcca aagacttact cttctcatta tggcgtgaaa
    19561 atgcgaatgg acatccatat agtggtgggg tatatagcgg agataattat atcctgtcgc
    19621 aaggaaatat cgactctttt gatacatctg ctatatttag caaagattca caggatgcta
    19681 tcatcatgga aagcaatata aaagctgaca aaactatcaa actgcctact gctgatctaa
    19741 gaaaccaaat ctatgacctg atggaaggaa ctactaagtt ggcaaatagc tgatatgatg
    19801 aaaagatagc aaatactact tctttgaata gcatttgctt aaaaaaagta ctcgactaaa
    19861 cgtaggaaag agtgtgggac aaatctaaaa atcaattttt gctacagact ttaattccaa
    19921 ataaacagaa cagaaagagg tgttcttctt tctgttctgt ccaaattatt ctaagatttc
    19981 tctttaccaa cattatttga caaagggcct attaaatcta aaatagtatc tttagattgc
    20041 taaaatatgt gctagcaaat cattcatggc ttgttcttat ttt
//
LOCUS       DACDIE010000048        19958 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.048, whole genome shotgun sequence.
ACCESSION   DACDIE010000048 DACDIE010000000
VERSION     DACDIE010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19958
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.048"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(20..955)
                     /gene="murB"
                     /locus_tag="IV250_001965"
     CDS             complement(20..955)
                     /gene="murB"
                     /locus_tag="IV250_001965"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAP8769229.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1028..1948)
                     /locus_tag="IV250_001966"
     CDS             complement(1028..1948)
                     /locus_tag="IV250_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8769230.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2061..2813
                     /gene="xth"
                     /locus_tag="IV250_001967"
     CDS             2061..2813
                     /gene="xth"
                     /locus_tag="IV250_001967"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAP8769231.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            3677..4210
                     /locus_tag="IV250_001968"
     CDS             3677..4210
                     /locus_tag="IV250_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAP8769232.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(4700..5224)
                     /locus_tag="IV250_001969"
     CDS             complement(4700..5224)
                     /locus_tag="IV250_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8769233.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(5217..5690)
                     /gene="tsaE"
                     /locus_tag="IV250_001970"
     CDS             complement(5217..5690)
                     /gene="tsaE"
                     /locus_tag="IV250_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAP8769234.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(5813..6793)
                     /gene="pta"
                     /locus_tag="IV250_001971"
     CDS             complement(5813..6793)
                     /gene="pta"
                     /locus_tag="IV250_001971"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAP8769235.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(6946..7626)
                     /locus_tag="IV250_001972"
     CDS             complement(6946..7626)
                     /locus_tag="IV250_001972"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP8769236.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            7752..8621
                     /locus_tag="IV250_001973"
     CDS             7752..8621
                     /locus_tag="IV250_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8769237.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            8647..9162
                     /locus_tag="IV250_001974"
     CDS             8647..9162
                     /locus_tag="IV250_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769238.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(9209..9772)
                     /locus_tag="IV250_001975"
     CDS             complement(9209..9772)
                     /locus_tag="IV250_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8769239.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(9827..10006)
                     /locus_tag="IV250_001976"
     CDS             complement(9827..10006)
                     /locus_tag="IV250_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769240.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(10083..10592)
                     /locus_tag="IV250_001977"
     CDS             complement(10083..10592)
                     /locus_tag="IV250_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769241.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(10688..11068)
                     /locus_tag="IV250_001978"
     CDS             complement(10688..11068)
                     /locus_tag="IV250_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP8769242.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            11398..12147
                     /locus_tag="IV250_001979"
     CDS             11398..12147
                     /locus_tag="IV250_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAP8769243.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSRGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKLTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(12331..12498)
                     /locus_tag="IV250_001980"
     CDS             complement(12331..12498)
                     /locus_tag="IV250_001980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769244.1"
                     /translation="MKDIQSGNVQETIDKWKGKHYRFTEAQKQALEYYQKLEESLRFN
                     DEARKYLKELL"
     gene            complement(12514..13608)
                     /locus_tag="IV250_001981"
     CDS             complement(12514..13608)
                     /locus_tag="IV250_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769245.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSK"
     gene            complement(13687..14331)
                     /gene="pcp"
                     /locus_tag="IV250_001982"
     CDS             complement(13687..14331)
                     /gene="pcp"
                     /locus_tag="IV250_001982"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAP8769246.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(14530..15474)
                     /locus_tag="IV250_001983"
     CDS             complement(14530..15474)
                     /locus_tag="IV250_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAP8769247.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(15476..16162)
                     /locus_tag="IV250_001984"
     CDS             complement(15476..16162)
                     /locus_tag="IV250_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAP8769248.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            16635..16955
                     /locus_tag="IV250_001985"
     CDS             16635..16955
                     /locus_tag="IV250_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAP8769249.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGLLLISIIGIKI
                     TSGH"
     gene            17116..18021
                     /locus_tag="IV250_001986"
     CDS             17116..18021
                     /locus_tag="IV250_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8769250.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSEGTIRIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVKQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDQAARTILTDAGYPLEDPDKIKLLKKNEELLGTVIQLLSAENVHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            18018..18791
                     /locus_tag="IV250_001987"
     CDS             18018..18791
                     /locus_tag="IV250_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8769251.1"
                     /translation="MTLLIKQELFKLIKKKSTAVLSVLLVALLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMESQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSIYLYLLAIIVTVLMKLILFPSISFTEKISTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIALFLALGYLVFKRKNV"
     gene            complement(18968..19879)
                     /locus_tag="IV250_001988"
                     /pseudo
     CDS             complement(18968..19879)
                     /locus_tag="IV250_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
BASE COUNT         5855 a         4072 c         3553 g         6478 t
ORIGIN      
        1 ttttaagttc cattacttat taattggcta aacttctgct tgtttctgat attgcttgtt
       61 tttctccaat gatccgcact tctgtttcta aagacacatc aaatttttct ttgattactt
      121 cttggatgtg cgcaatcaat tcaatataat ctgtcgctgt cgcatgatcg atattgacga
      181 tgaatccagc atgtttttca gagacttgtg caccgcccca tttcaggcct tgcaatcctg
      241 cttgttggat cagctgacct gtatagtgtc ctactgggcg cttaaagaca cttccgcatg
      301 aagggtattc taatggctgt tttgattgac gcaattcggt caactcatcc attcttgctt
      361 tgattgcttc ataatcccct gtttgcaaac tgaatcgagc ttccaatacg atagcacgta
      421 attcttggac tttactgtga cgataagaaa ataccatttc gtcttttgtc aaagttttca
      481 atgttccatc ttcaagcaaa agatccactt cagcaaaaac atctttgatc tctccgccat
      541 acgcacctgc attcatataa accgctccac cgacacttcc cgggatgccg caagcaaatt
      601 caaatccagt caatgattct gccaaagctg cataagtcgt atcaatcagt gaggcacctg
      661 cttccgcaat gaccatcgtt cctttgacat gaatctgttt catttcagtc agcatgatga
      721 ctacaccgcg aatcccgcca tcttgaacga tcagattgct tgcatttcct aataccatcc
      781 aaggtatatc atggattcgg caataatcga tgatttgctt tacttcttct tgttttttcg
      841 ggaatgcaag gacatcagct ggtccacctg ttttcgtaaa cgtatagttc atcaatggtt
      901 catcaaacaa tatagtaatc gtggctaagc tttcaactat ttcttcttta ttcactgagg
      961 attttccttt cttttctcaa acgtcctcat tattctatca cgcttaatac aatcttgcca
     1021 gtcttgttca aagttgatga cggtagtgat ctttaagcac ttcctcacat aattcatgac
     1081 ttcttacaac ttctttttgt ttaagcaatt ctgtttccga tttacctaaa cggatctgag
     1141 aaaagaaggc ctctaccatt ggctcaaacc cgcgtttgac tagcgtgttt gtccagtcgc
     1201 caaattcttt cacttttttt ccatcagcat cttgaatgac aagctgggtc agatcgttta
     1261 cttcataagt accattttta cttgttactt gatatttttc tatattagcg ccacttgcta
     1321 aatccatcgt taaaatagcc gtttgatttt gtgtttctaa ttgtaaaatg gcatattcca
     1381 aaaattcctt tgtttctctt atctggtatt ttgttctcaa aatgggttca tctaataaat
     1441 acacagctgt atcaataaga tgtaaaaaca aatcataaag gacgaactct gtcgtttctt
     1501 ttgccgctat tcgatttttt tgcaggtgga tcaaccgctt atctgggata cgtttcaatt
     1561 cttctaccat aggagcaaag cgccggttga aaccgatcat caaaattatt tggttcttat
     1621 cagctaattc ttgcaattca cgaacttcat ttagatggac actcagtggt ttgtcaatat
     1681 aaacatggat accattttct agacattttt tggctaattc ataatgtact tttgttgctg
     1741 cgtggatcat acatgcttcg attttttctt ctaaaagctc atcgatcgtt tcatatagat
     1801 gttggaaccc gtactctttt tgaattcttt cttttgtttc tttatttctt gttgcaaaat
     1861 aaaaatcaag tgttccttgt tttttcgcat aagttggtaa atatgctttt tctgcgatat
     1921 tccctacacc gattacacca atcttcatct actttcctcc tggatcattt agttactttc
     1981 atcttacacc attttttact aaaatgaggt acaatagtat cgcaaaggta aggaaattaa
     2041 ttcgaaggag tgtgctttta ttgaaattta tctcttggaa cgtaaacggg cttcgtgcca
     2101 tcgtaaataa aaatttttta gaagtttttc atgagttaga cgccgatttt ttctgtctgc
     2161 aggaaacgaa actccaagca ggacaaattg atttagatct tccaggttat tatcaatatt
     2221 ggaattacgc ggaacgaaaa ggctattccg gcacagcgat ttttgctaaa aaaccagcat
     2281 tgaatgccac ttacggaatg ggtatcgata tccatgatac agaaggccgt ctgattactt
     2341 tggaatattc cgatttcttt cttgtaacct gttacacgcc aaattctcaa agcgaattaa
     2401 aacgtttaga ttaccgatta gaatgggaag aagcctttta taattatttg gaaaatctaa
     2461 aaaaacaaaa accagtcatc gtgtgtgggg atctaaatgt cgctcatcaa aagattgatt
     2521 tgaaaaactg gaaaacgaat caaaaaaatg ctggattcac tccagaagaa agagcggctc
     2581 tttctcgact gttggataac gggtttatcg atacattccg ttatttttat ccaacccagg
     2641 aaggtgtcta ctcttggtgg aactaccgat tcaattcaag gaaaaacaat gctggttggc
     2701 ggatcgatta ctttttgacg agcaaagatt tagagccagg actagctgat gcaaagatcc
     2761 atacggacat tatgggcagt gatcattgtc cagtagaatt agatttgaaa taaagaggtt
     2821 gtgaaaaagt cgtttagttt cgagtattaa gaaaaatttt gtgaaaatgg ctttccacat
     2881 tttgcataaa tttggcataa taccgagaaa ctggcttttg aacactgttt atcgagagat
     2941 tgtgaaaaag ctgtcctgca tcaagtaata agaacagcca aacaaaaata gttcctcata
     3001 tttttcgttt ggttgggctt attaccgcag atacaagctt ttgaacaccg tttattaaga
     3061 ggttgtgaaa aagctgcact gcatcaagta ataagaacag ccaaacaaaa atagttcctc
     3121 atatttttcg tttggttggg cttattaccg cagatgcaag cttttgaaca ccgtttatta
     3181 agaggttgtg aaaaagctgc actgcatcaa gtaataagaa cagccaaaca aaaatagttc
     3241 ctcatatttt tcgtttggtt ggacttatta ccgcagatgc aagcttttga acaccgttta
     3301 ttgagaagtt gtgaaatcag cgtcctgacc tgagtattag agaaacaaat ggcaaaatag
     3361 ttcctcatat tttgctattt tttgagctaa taccgagggt cagctgattg aacaccgttt
     3421 agtggagaag ttgtgaaaaa gtcgcctact ttcgagtcgt aagaaatttt tttgaaaatg
     3481 gttcctcaca actttgcata aatttgactt aataccgaga agtcatgatc cccatctaac
     3541 tccaaatcac aaggaatgag ttcaaaaact agcttttctc aacaaatatt acataaaaaa
     3601 ttctatgttt tttgttaaat ttatttgctt ttttcaaaag tatgttacag taatttccca
     3661 aggaggtttt acagaaatga attttgttgc catggatttt gaaactgcca atcatcagcc
     3721 ttatagtgct tgttctcttg cgctagtcat ggtaaaaaat agccagatcg ttgatgaatt
     3781 ctatacattg atacagcctg agactccttt tttctggcga aatgtccaaa ttcacggaat
     3841 ccatcaagaa gacgtacgca atgcgccgaa attcccagaa gtctggcaaa ctatccaacc
     3901 atattttcaa aaaaatcgtt tagtcgttgc acataatgct gcttttgata caaaggtctt
     3961 agcgggatgc ttagattatt atcatcttga acagcctaat tatctttctt tatgtacagt
     4021 gaaaagtagc cgtcgcctgt ttcctgaatt tccaaatcat cgattgaaca ctgtgtgcga
     4081 aaatttgaac atccaattag atcatcacca tgatgcattg gaagatagtc gtgcatgtgc
     4141 ggaaatcctg ctttatcaag aaaaaaaatt cgggacagat ccattgaaaa aacttgtgac
     4201 tataaagtaa aagaatactt atgaaacaca caaagaggtc gtgagcctgc cgtttcactc
     4261 cgagtcacaa ggagcaattt caaaaaatag cttctcatgt ttattaaata ttgtgactgg
     4321 tgaccgagga gctggcagga gaacaccgtt tattggagag gttgtgaaaa agctgttctg
     4381 catcaagtaa taagaacagc caaacaaaaa tagcttctca tatttttcat ttggttggac
     4441 ttattaccgc agatgcaagc ttttggacac cgtttaccaa gaggttgtga aatcagcgtt
     4501 ttgacctgag tattagagaa acaaacggca aaatagttcc tcatattttg ccgttttttg
     4561 agctaatacc gaaggtcagc tgattgaaca ccgtttattc aaaaaaaatg gactgcgaca
     4621 cgacttttgt cacagtccct tcgtgtgttt ttctgccttc tcaaaaggat gacagtttct
     4681 cactcttttc ctatttcttc tattcaatca aataactcat tttatataca tctaaaaatt
     4741 cacctagatc tgttcttgcc cctctaggca tgaccgcttc gatttggaag cccatttttc
     4801 ggtaaaggtg tacggctctt tcattacgaa cttgaacgtc aagctccaat cgaaaaagta
     4861 cacccatttc cttcgcccaa ctgatgattt cttctagcat aagagtgcct agtcccattc
     4921 cccaatattc ctgcagaata ctgataccga cttctcccac gtgagacaaa cgaaattgac
     4981 tgtctgcctt gacagatgct gttccaataa tcgtcccttc atagattgca agcagcaata
     5041 aattattatt gctttcgtat atataatcca actcttcttc taaggtttct ggtggtaaca
     5101 ggagttctgc ttcatccaaa actaaaaatt ctgtctcctt attaacttgt cccatcatca
     5161 aaagaatgtc ttttgcatct cctggcactg cttcccgcaa gatgatccca acttcttcac
     5221 tcatgatttt cagcccattt ttctagaatt cgctgtttga aggtttcact ttgctccccg
     5281 tatgagatta gtttcacata ccgcttttct aaatctgtat cacttttttc taaaatcagt
     5341 tccaaataat cctccggcag tgcttcttct aatagatttc cccattcaac aacggaaagc
     5401 ccatcacctt caaaatattc atctaaccct aaatctgctc cgctagctgc tacccgataa
     5461 atatccatat gatacaacgg aatccgacct tgactatatt ctctaataat cgtgtatgtc
     5521 ggacttttga tcatttgcgt tatccctaat ccttgggcaa ttcctttcgt caacgttgtt
     5581 ttacccgcac ctagatcacc agtcaatatc agattgtcac ctggcattgc ggaatgacca
     5641 attgcctctg cgaattgttc tgtcattgtt aatcctgata gttcgatcat ttttcatctt
     5701 ctcccgctct tctttctttt tgccagtata tcgcgtaatg aagttttttt aaagaaaaaa
     5761 tgaactagtc aagaacagca gctgctgttt ttggctagtt cgcgtgtatt tgttaatcaa
     5821 tcaatgtttg tgctgctgtg ataatcgata atttatatac atcttcttca tttgctccac
     5881 gagacaaatc agaaaccggt ttgttcaagc cttgtagaat tggtccgatt gcttcaaagt
     5941 taccgaaacg ctgagcaatt ttatagccga tatttcctga ctgcaattct ggaaatacaa
     6001 agactgttgc ttgaccagct acttctgaat ctggcgcttt cagttgtgca actgaaggaa
     6061 cataagaagc atcaaattgc aattcgccgt cgatcatgta ttgtggcgct agttttttag
     6121 cgattttagt tgcttcaact actttgtcta cttctggcga tttcgctgag ccttttgtcg
     6181 agaagctcat cattgctact tttggttcga tatcgaacaa ttctgctgtt tttgcagaat
     6241 ctacggcgat ttcagccaat tcttgcgcag ttggattcac gttgatcgca caatccgaaa
     6301 agacatattt ttcttgatct cttccacgta ccatgatcat cgctccgctt gtccgactga
     6361 tgccaggctt tgttttgatg atctgaagtg ctgggcgaac agtgtcgcca gtcgaatgga
     6421 tcgctccaga gaccattccg tctgcaatcc ccatatacgt aagcatcgta ccaaagtaat
     6481 tcacatcttt taaaatcttt tctgcttgtt ctttggatgc ctttccattt ctacgttcaa
     6541 caaaagtatc caccatttca tcccacttat cgtaattatc agggctgatg atcgtaaagc
     6601 tagatgtttt gattcctctg gcatgcgctg catttcttat ttcttcttcg ttccctacta
     6661 aaatgggttc cacaagttct tctgctttca accgggcagc tgcgcctaaa atacgagcat
     6721 cagtagcttc cgggaaaacg attttgatcc cacggcgaac aattttaaat cttaaactat
     6781 caaacaattc cacaagatga cctccagacg tttttatatg tattatcata cactattcca
     6841 gaaaaaaaga acagtttatt ttatctgtat cagtcaaaaa tgctggaaaa attaagtttt
     6901 tcacatacaa agcgaactgc actataacag gaaagcgctg ttttattaca ctgtttcagg
     6961 cagttgccaa tcaatcggtt cttctcctaa cgcaaggagc gcatcattcg ttttcgagaa
     7021 gggtttggaa ccaaaaaagc cacgacttgc agataacgga ctgggatgtg gagatgttat
     7081 gatgatatgg cgactcttat cgatcatttt gattttttct tgtgccggtt ttccccacaa
     7141 gataaacacg attggttttt cccgttcatt taatttttca atgatcgtgt ctgttaatct
     7201 ttcccaacct ttcccgcgat gagaatacgc ttgcccttcc cgaacggtaa gaacggtgtt
     7261 gagtaaaagg actccctgtt tagcccatga cactagattt ccatgttgaa caggtgcgat
     7321 tccaagatca tcataaagct ctttatagat atttcttaac gatggcggga tctttacgcc
     7381 aggttgcaca gaaaaagata agccatgagc ttgattttct ccatgataag gatcttgtcc
     7441 taaaatcacc actttaactt cttcataagg agtcaattcc aatgcttcat agatatgata
     7501 catatctgga tggatctttt gggacgcata ttcttttttt aaaaattctc ttagatgcag
     7561 ataataatct tttgaaaatt ctgaggacaa aacttcttgc caactgttat gaatgatttc
     7621 tttcaaaaac tccacctcct aataaaaaga ataactcaca ttttcttcaa tcacaaggga
     7681 caatcttcac cctcagcact tgattaatat ggtaaactag gaacaagaag cagaaaggaa
     7741 cgataatcat tatgatcaaa ttaattgcat cagatatgga cggcacatta ttagatgccc
     7801 atatgagtgt ttctcctgaa aatgccgagg cgatacgttt tgcaaatgat gccggtgttg
     7861 agttcatggt cgctactggt cgaaactatc aagaagctcg cgcagcttta gatgaggtag
     7921 gaatcgattg tgcgatgatc actttgaacg gggcacaggt atttgataaa gaaggaaatt
     7981 cattgtttac cgtgtcgatt ccaaataatc aagcaatctc tgttttagat attctagatg
     8041 ctaacggtat ttactatgaa gtagcaacca acgatggtct ttattctgaa aatcaagcga
     8101 aacgaattga aagttttgct tccatggtgg ctacccattt acctcacctt acgtataaaa
     8161 tggcgattgc catggcctca gctaagctgg agttattgca catcacatac gtggacagta
     8221 ttcgtgctat tttggaagaa aagaatctgg aagttttaaa aattatttgt tttcatacag
     8281 aagggccagc tgtattaggt ccagttggaa agcagatttc tcttttaggc gatctagccg
     8341 ttacttcctc tggtcaaaac aatatcgaag tgaaccataa aaacgctcaa aaaggaattg
     8401 ctgttgccca tgtcgctcac gagcgtggga tttctttaga agaagtcatg acgattggcg
     8461 ataacttcaa tgatgtcagc atgctgcaag tagcaggagt cagtttcgca atgggaaatg
     8521 cagaaatcga agtaaaagat tatgccaaat atacaacgga cacaaacctt gaatctggcg
     8581 taggaaaagc catattgcgc gcgattgcag aagacttgta gttttatcta gaaaagaggg
     8641 atatagatgt cagaattttt cattcaggaa aagcagcttg gacgtgcaac tcggacaatt
     8701 gtcaaaaacg aaaaaggaca ttccctgttt ttgatggttg gtcgctgggg aacaaagggc
     8761 gatgcattgt ctctttatgc catgaatggc gatctcgttg ctcacatcaa gcagataagc
     8821 ctgacattcg gtacgcgttt tgagctgtat aaagagttcg aaaaggtggg aaccatgcag
     8881 aaaatattca attggcccgg tgacttttat tatattcgta gacttcactg gacagtccaa
     8941 ggtgatatct ataatcatca atatcaaatc catcatttca atcagcaaat catgaaaatg
     9001 gataaagcaa cacttttttc aggagattac tatgtattgg atgtctcaaa tgatgaagat
     9061 gctccagttt gtatttgtat tgctgcggtt ttagattact ggctctaccg taaagacaaa
     9121 agtcgcgata agcctgtttc ttaccgactt aacttaagtt aaaaagatgt acctgaaatg
     9181 cggaaatacg catttcaggt acatcttttt agatattctt ttgcatatga tcataaagat
     9241 gaccactgat catccgctga ccgacaactt tgaagtcatg gcgttcgtat aattttttag
     9301 cctttggatt tttttcatcc acacttagac cgatgacgga gcgattagct ttcttcgcca
     9361 atttaggtaa tgcttctaat aatcgcgaac cgatgccttg tcctctaaaa tcttctgata
     9421 cactgataga gtccaaatac cattcgttgg gaaacgtttc tttatcaatg aacaaacgtg
     9481 tagacgcatc tagctgatat ttctctaaga taggtaccag tggctgatcg atgattgatt
     9541 cttcttcatc tgtataaccg taagcaactc ctgcaacttt accgtctatt tcttctacga
     9601 tacctcgtga ataactgtaa cgataatttg gatcagtgat tgcttcttct aaaatctcta
     9661 aagtcttttg ttcccctgtc tgcttcaaaa atggcagttc catatctttt agaatcacta
     9721 agatcaatgg ggcgatttct ggagcatctt cttttctcgc aaaacgaatc atagtttcct
     9781 ctccttttct ccattctaac atgcagaaaa agaaatcgca aaatcgttat tttaaaaaat
     9841 cttcaatctt ttcagtaatg aaataaccat gagtaacgat tcttgccgct tcagttcggc
     9901 aataagggca gacgactggc atttctttat cattatgttc gactacatta ttgacttctt
     9961 cgtatgcaaa tttttttccg caattagggc atttcatttt ttccatgtta tctcaacttc
    10021 ctttcatctt tactctctta gcttactctt atattgtaaa aaagagaagc aatatccttc
    10081 ttctatgcag atatccatac ctgacggtcc tgttctccat ctattaatcg gactttataa
    10141 taaggacgtt catctttgac tagaagtgtc cactctttca cttttgcttt tttcccgtag
    10201 tagctgactg cttccttcat cgctgcttgc ggttgtttga actcttccgt tttttctata
    10261 tcaaaaagat gttctgcggc cttttccgtg tcatttcgta cggttgtttt cccagtggat
    10321 ggattgactg ctatgacttg tccttcacaa aaaaactggt attcatatgt tgcattcgta
    10381 ggattctcca aatctttttc aactgcaaaa tgaatagcag atatgttatg ttcttttgtt
    10441 tcgtttttta cgattgctgc tgcttttttc aatgagatcc gatattcctg gtcactaata
    10501 tcatccatgt gaaaagcggc attgagcagt cccgtcatac cggtaatccc tattacgatg
    10561 agggcagcaa caccaaacat catttttttc attcttcatt ctcctgtttc ttttttttaa
    10621 agaatatcaa gaaatgaacg aatgaaaaat cgaatgaaac gaaaaaaaag tgacaatttt
    10681 gttatcttta ttcgtgcagt tctaatggca atccgtctgg atcaaagaaa aacgtcattt
    10741 tttttcctgt aaaggtatct tctctaatcg gttctgattc gatccctaag gactgcaatt
    10801 ctgcaattgt ttcttcaata tcgtctactt tgaaagcaag atgacgcaag ccacaagctt
    10861 ctggatagtt tggtcgttgt ggcgcattag caattccaaa aatctccaat tcagcacttc
    10921 ctagtttcag atctaattta tagtcatctc gttctttgcg gtaattttct ctgattactt
    10981 caaatcctaa tttattaaca taaaaatcac gtgatttctg ataatctgaa acaatgatgg
    11041 ctatatgatg gatcgttgta aggttcatat gagcactcct attcttcgtt taagctcgga
    11101 tctatattaa tctccagttc cattgtaaat gtgtcagatt gaaaaaaata gctgtttttc
    11161 ctttttataa gaaaaaaggc aaaaaaaaca gaaagtaggt cctccaagac actcttcctg
    11221 ttcccaatat aataaatgaa agtaagaaat tatattttca agaaaacaaa cagaaaatta
    11281 tcataacaca aattatttat aattgaaaga ctagtttttt ttcaagaaaa gcatacaata
    11341 gattgtgtta gtaaatacag aacacactac atttagtgtc ttaaggagtc ctcatacatg
    11401 aagtcatatt ttcagttttt agctcatcag ctaaaaggct ggccgcaaca aaattattat
    11461 cttttctttt tcagtcgtgg ctgccaggtc atgactcttg tcaatagtcc catcacttgg
    11521 ctgtccgtta ttacatttat cggtaccacg cttggtgtat tgtgtatttt atccatcaat
    11581 gcggcaaaat cagtcaacgg tattttaggt attttgtctg cgatttgttt tattatcgtt
    11641 gggttttcag caaagaacta tttaagtatc ggtgaacaga ttgcttatat gatcacattg
    11701 gatatccccg tattgttaag taaagaatgg aatcataata tggcttcaaa aatccgaaaa
    11761 ttaactggaa aaagctggtt tatcgcaatc atcagtactt tccttgtcta tcttgtttca
    11821 ggattcctga ttggtcgtta tacagatgat cctcggccgt gggtagatgc cattgctttt
    11881 tctattagct tgactgctgg tatcatcagt ttcttgcgtt acaacaacca atattactgg
    11941 tggttagctt cagggcttgc tcaaatggtc ctttggtata tttcatttac gcatggatct
    12001 gcaagtttgg cgatgttcgt gaacagctct gtttacttgc tgaatgatgt ccttgcattt
    12061 accgtttcac cttggtataa ccaaaaagaa cgtgaacgta tgcaaaaaat ggaagaagaa
    12121 tactacaacg cacaacaaca cgcataaaaa aactgagcgt gggacaaaag taaagaacac
    12181 ttttgtctca cgctctaaac acgtataaac ggcggaatca gaagcaactc cttcggaaat
    12241 aagctgaaat tcacaaaaat ttgaaaagca attttcgtga atttcttctt atttcttgga
    12301 gttaaacgct tctgtcccag cctctatttt ttatagtaat tccttcagat actttcttgc
    12361 ctcatcattg aaacgcagtg attcttctaa cttctgataa tattccaacg cttgtttctg
    12421 agcttcagta aaacgataat gttttccttt ccatttgtcg attgtctctt gcacatttcc
    12481 agattggata tctttcacta ctagttgcca tttttatttt gagactgtac ggtctttctt
    12541 atcagcagat tgtatagctt ctcgttcttt acgcaatact tttaaaaaac catttctgac
    12601 tatccgagga atgctgtcga agaacccttc tgtaaaacca tagcgacaat attgcttcca
    12661 aataaataga tagatttttg gttggatgaa tcgttggttt ttcgcaaaat attccatctg
    12721 agcagtgact tctggtgttc gttgacgttt tgtctttctc aacacggaaa taaatgtttc
    12781 aaaataagca ttaggatgta ctggcacttc ttcatatacg atggattgcc aatctttacg
    12841 taaaaatgcg cgatagttga tcaagaattc ttctacatcc tttactaatt cttctgcaaa
    12901 gtcgtcaaat agttcttttt ttatctgtcg cttttttgac tgcccattga tgtgttcact
    12961 tattttcttg atttcatttt ccaatgcttg ataatccgct atgtaaatcg gcatttctac
    13021 ttggcgaaat tctgcgagcc gctgagctaa ttttatatcc ggtaaatatg gctgtttcat
    13081 ccgacgattt tggtacaaga caaaacagta tttttgccat agtccttcag gaaaatcgat
    13141 attcttctgc ttaagccata ataattcgtc taaggccaga tcaacttttg tcattccctg
    13201 atggatcgaa gctgccactg tcggagagac atgcaggtga tcggcaaagt ggttgatgcc
    13261 tagttttagg atctttccgg tttttggcga tttaacgatg tattgcactt ttaattctct
    13321 accacactca caatacaatt tttgctggct tcgttttgtt cgaaaaaacg gattgatctt
    13381 gaagtcagaa aacacccaat ctgttgcagc taaataatgt tcttccataa aacgagagct
    13441 gattagatat cggcggtgct tatcgactag ttcccgctgt tttttcgtta attttgcata
    13501 gatctcccaa cgttgactgc ttgtgtattg tttagaaatt ttgatgggat agcttttgct
    13561 gatttctttt ggaaaccatt cttcaaagac ggattgccat tcttccacgg ctgtttcttt
    13621 cctttctaat tacaaaaagc tggctactaa cataacgtta gcaaccagcc agtgtttctt
    13681 gctaaattag tgagttgttc cggcatttcc ggaaatatct tctttatttt ctactgctgc
    13741 ttcaactgca taagtcaaag aagaagcaat gtcttctaaa gacatagaag gcgtacccac
    13801 tggacgatcg atcacttgat cgggtgagaa tggcacgtgg atgaacccac ctcgaattgc
    13861 cgggaattgt gtttcgatca aatgaagcaa acgatacatg atatcattgc agacaaatgt
    13921 tcctgctgta taagagatgt acgctggtag accatgatct tgtacatttt ttgtcattgc
    13981 tttgattggc aatgaggtaa agtaagcggc tggaccgttt tcttctaaag ccgtatcgaa
    14041 tggctgattt ccttcattat cagggattct tgcttctgct agattgattg cgacacgttc
    14101 gaacgagact gctgaccgcc cacctgcttg tccgacacag atcacgacat ctggttggtg
    14161 ctgttggatg gcttctttga ctacttgact gcttttttcg aatacggtcg gtacttccac
    14221 tttgatgatt tcagcaccgc tgatttcatc tggcattttt tttactgctt cataggctgg
    14281 attcactttg tccccaccaa atggatcaaa acctgttact agtactttca tctgttgttc
    14341 ctccgtccga ttgatttaac atatggttat cttttatcat accattttcc ttgaacagcg
    14401 taaataaagg aaacaatagt ttaaataaca taacagatca aacttaatat attcttataa
    14461 aaaaatagtg ttccactaca atgttagaag aggttgcgac aattattctg tcacaacccc
    14521 tctcttctat tatttaaata gaatcatata agcaatttgg aaaactagca taaataacgc
    14581 aataaacaac tggtttttga tcactccgta tttgtctttc atctccagca tcgctacagg
    14641 cacgatattg aagttagcag ccatcggtgt cagcagggtc ccacaaaaac cgcaagtcaa
    14701 tgcaaccatt cctattaacg ctggattcgc tccatgacta aatacgaatg gtgcaccgat
    14761 cccaaccgtc atcacggtga tggccgcaaa tgcattcccc atgattaccg taaatatcac
    14821 catgccaaga gcgtaaatga tgatgccgat gttcacattt ccttctggaa cgatcgtacc
    14881 tactgcattg gaaatcactg tcccaactcc agcgcttgta aataccgcac ctagagaagc
    14941 taaaagcatt ggcaacatgc ttaacggccc tactgtgccc agcatatctg ctgcatcatc
    15001 taaaaatgta cttggtcgat tatctcgaga aaagaacatc aagattagca tggctgcaaa
    15061 aacaccgacg cccacgccga ctaaagcacc taaattggta aatagagcaa acaagatcgc
    15121 aaagacaccg atactcaaag ctggaataaa aattttcatt cctagtttgt ctgacatttt
    15181 ttccatatat tcttttgatg gaagccggct ttctcctttg ctgactttct tagcaattgc
    15241 tggtatggtc atcgcaaaga tcaagccacc gttcacgatt gccggaagcc atcttcctcc
    15301 tgcaataact acccctaaga ccgtccagaa aaaggctgac ccataacgat gaggattggt
    15361 tttatctgtt aaatttctta ctccagtgta aatcaatacg agtcccatca cgatataaag
    15421 gatctccagt aatttatcgc ctaataaaat atcaggattt gtaaaaaagt ccatcttatt
    15481 gcccctttcc tggataatat ttcttcgcta atttcttttc tttaagatag aagtagacac
    15541 ttgccacaac taaagccgtg atagccactg gaacttccac taaagcaagc tgtcccaatg
    15601 ttacgtcata tcctagatct ttcagtgttg attggactag caacgcccct gctcctccga
    15661 tgaatagtac ttggccaaag aaccagcaga cattttccat agctgaagcc atacctttta
    15721 gttcttcttc atgcttagga tggacttcta acccttttga ttcgatggtc cccaatgcca
    15781 tcggtaaaat gattggacga acaaatccag caactccacc aaaactcacg ttaaaagccg
    15841 caaagatcat tcgaaagatc ccataaatat ctacaatcat accagcagat actttcttga
    15901 atcgcttgat caaggaagcc gctgattctt tcagaccatt tctttcaagc gttcctgttg
    15961 ccaacataat gatgataaaa atcgccatcc cgcgattgtt tacaaaactt gtacctaaaa
    16021 gttctaacat tccttgaatg ccaagaccgc cagtcaaagc tgtaacaact aaagcaacca
    16081 ttacgatcaa tatcgagtct aatttaaatg caaacccgag aataacaatc aagacgccta
    16141 aaaggctaag ataattttcc atatattttc ctccataaaa tgacaatatt tttttaatta
    16201 taaagcaaaa cacataaaaa atatagttgt tttctcacga aatagacact atttttaaat
    16261 atttttcgtt atttttttat tatataattc accttactta ttgtaagata aagctttttt
    16321 taatttattg cctgctaata tataaaaata aggtctagtt actgtttttt taaatgatta
    16381 ataaataaca actcctttct atgaaattgc aaagcattca tcttcttttc tgctaacgat
    16441 atatccgtca atgcttgtta cactttacta aaaagtatga gattgccgat atctggatag
    16501 gtgtgctata ctacagaaaa agatatacga tctgccaccg ggtagtaaaa aagaggatga
    16561 tcattccatt aggtctttga agggatgatt ctcctctttt tgtgtatttt ttatattgaa
    16621 ggaggaacgt atgtatggca tggatggaat taatcatagc aggaattttg gaagtattct
    16681 ggtcaacgat gatgaaatgg agtgatggtt tttctaaaat caattattct ttttacacag
    16741 tgattggaat gattgctagt ttttactttt tgtcaaaggc aatcaaaagt ttgcctatga
    16801 gtctctctta tcctatttgg acagggattg gcgcagttgg ttcggtcatt attggtgtgg
    16861 tcttgtttca tgataagctg aatctttcca cttggttctt tgtcgggttg ctgctcatca
    16921 gcatcattgg catcaaaatc actagcggtc actagatact tagacaagca gacaaggact
    16981 gatcagaaaa agcggggctt tttatttgat attcctcttt ctatgaaaaa acaagaaaat
    17041 aagactaatg acctagcgac ttctctactc atcaggtgta tgcttgtact atctatagaa
    17101 aaggagtgaa tatttttgaa aagtctttcg gtccaagaat tatccaagaa ctttggtcac
    17161 aaacaagccc tcagcgatgt cagttttagc ttatctcctg gcaaaatcgt tggattagta
    17221 ggtcctaatg gcgctggaaa aacaacgatc atgaaagcta ttttaggact gatccattac
    17281 tcagaaggaa cgatccgtat tggtgaaaat gagatttctc cctctagtca taaaggatta
    17341 gaacaagtag gagcattgat cgaatatccg ggtatctatc cattcttaac aggttatgat
    17401 catctgaagt tattctctga aacaaacgat gtatctgcta tcgacactat cgtgaaacag
    17461 ctcaagatgg aaaaatacat tcacaaaaaa gcgaaatcct actcgctggg aatgaaacaa
    17521 aaattgggaa ttgctcttgc cttactgaac catccgtcta tcgttatttt agatgagccg
    17581 atgaacggtt tggatccaca agcaacaaga gatgtgcgaa aaatcattac cgacttggct
    17641 actcagggca cctctttctt gatctccagt cacttactca gcgagcttga aaaaatggtc
    17701 gatgagctga tcctgatcaa tcaaggaaaa atcatcaaac aatgtacaat ggctgaattg
    17761 aaccaaacag cgcaagattt catcgttatc tctacgacaa atgatcaagc tgctcgaacg
    17821 atcttgacag acgctggata tcccttggaa gatcctgata aaatcaagct tctaaaaaaa
    17881 aacgaagagt tattaggaac tgttatccag ttattgtctg cagaaaatgt ccatgtgaca
    17941 gatgtccagc atctgacgaa tgatttggaa tcttccctat tggaaattct ggattcagta
    18001 aagggggaag aagaaaaatg acgttgctta taaaacaaga attatttaaa ttgatcaaga
    18061 aaaagtcaac tgctgtttta tctgttctat tagttgcttt gctcatagga acagcacttc
    18121 tagccaaaaa atacacgacg atcattgatc cagttgaaat gactgctcag cttttcagtg
    18181 ccacatcttg gattgtcttt atcatgattg ctgctgcaag tacgatcatt tcaatggaat
    18241 cacaatatgg tacattgaaa aatctccttt atcgaaagta ttctcgagga gaaatattag
    18301 taagtaaatg gatcacatta gttatttatt ctatttattt atacctttta gctattattg
    18361 tcaccgtgct gatgaaactg atccttttcc cttcgatttc ctttacggag aagatttcta
    18421 ctggtcagac actgatccaa agcttattca cttatacttt gggtagttat atcggtttat
    18481 ggctaatttt aagtttagtg ttgatgattg cttgttttat caatagttcc ggtgcctcca
    18541 tctcagcagg gatcgtcttt tattttgctt catcaatcat atctggtatc ctcatcgcac
    18601 tcattcaaaa atgggagtgg atcaaatgga atccaatcag tatgctgaat ctgcaaaacc
    18661 aaattggcaa cgaagagatt atgaagcaat tgactcatct ttctactaat caaatgctgt
    18721 ttggcaattt agcttacatt gctttgttct tagcattggg atacctcgta ttcaagcgaa
    18781 aaaatgtatg atggttcatt aacaaagaag ttgtgacaga agtggccagt ttcaagaaat
    18841 ttcggcttat ttttagaaga agctacttct tgaacaccat ttattcaaaa aaagaagcga
    18901 tgacacgaat gttgtcaccg cttctttttt gaataaacca caggcttcac agcctctctt
    18961 tatacttcta tacgtgaagc atcccagtgg caaataaaac cattgcgatt actccgacca
    19021 tcgtcacacc gataagtaaa atcttatcta agtggatatg ttgtacaggt tcgttttgtt
    19081 ctttacgtgc tttgaggtaa aaataaatac ctggtaagaa aagaacaaga acaagcatca
    19141 cttcattaaa ggctacaact agcatggccc atacttgaaa agcacttgct aaaaacccta
    19201 ctaaaaattg gacccactct ttccgctgat aagaaagctt catttggtac agccctaaga
    19261 aaagccacgt gaagaaaatc gctgctgtac caagtgtata agcaaagtta tatgcactgt
    19321 cagtgaataa taatgaaagt aaaaagacat tcgtacataa cgtcgtcaat acgatagcat
    19381 aagttggtgc cccatttttg ttgagttttc cccatgcttt aggcaaaagt ccgtcctcag
    19441 ccatattccc cattgtttca atcggcaaga ttgtccatga gagccaaacc ccaactaaag
    19501 aaatgatcaa accgccgttg atcacgaccg ccccccattt accaaccatc atttcaaata
    19561 catatcccat agcaggttgc gctttgatcg tttgcagctg attcatcgtc attgtgccat
    19621 atggaagcat ggaaaccaag acgtaaataa tgatcaaacc gaatattcct agaatcgttg
    19681 ctttcccaac atccgattgt ttttttgctc gtgaacctac gacagaagct ccttcaatac
    19741 caacgaatac ccagagcatc accatgatgc aactcaatac ttgactagaa aacgggatcg
    19801 gctgcccatt cgcattcaat atgatatcgc tagaaaagtt attaatgaat tgttggatcg
    19861 aaaaggtgct gatagctacg taagcgcccc atagaacagt accttactaa caaatgcacc
    19921 tcaagatata cttgagctat caagaagtct ggaggttt
//
LOCUS       DACDIE010000049        19752 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.049, whole genome shotgun sequence.
ACCESSION   DACDIE010000049 DACDIE010000000
VERSION     DACDIE010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19752
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.049"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            149..1600
                     /locus_tag="IV250_001989"
     CDS             149..1600
                     /locus_tag="IV250_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP8769252.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            1949..2581
                     /locus_tag="IV250_001990"
     CDS             1949..2581
                     /locus_tag="IV250_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HAP8769253.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLTGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            3169..4608
                     /locus_tag="IV250_001991"
     CDS             3169..4608
                     /locus_tag="IV250_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8769254.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4903..5121
                     /locus_tag="IV250_001992"
                     /pseudo
     CDS             4903..5121
                     /locus_tag="IV250_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            5370..5585
                     /locus_tag="IV250_001993"
     CDS             5370..5585
                     /locus_tag="IV250_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769255.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5802..6058)
                     /locus_tag="IV250_001994"
                     /pseudo
     CDS             complement(5802..6058)
                     /locus_tag="IV250_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(6245..7156)
                     /locus_tag="IV250_001995"
     CDS             complement(6245..7156)
                     /locus_tag="IV250_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8769256.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(7175..8179)
                     /locus_tag="IV250_001996"
     CDS             complement(7175..8179)
                     /locus_tag="IV250_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAP8769257.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(8176..10299)
                     /locus_tag="IV250_001997"
     CDS             complement(8176..10299)
                     /locus_tag="IV250_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP8769258.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(10304..12751)
                     /locus_tag="IV250_001998"
     CDS             complement(10304..12751)
                     /locus_tag="IV250_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP8769259.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(12738..13127)
                     /locus_tag="IV250_001999"
     CDS             complement(12738..13127)
                     /locus_tag="IV250_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8769260.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(13187..13690)
                     /locus_tag="IV250_002000"
     CDS             complement(13187..13690)
                     /locus_tag="IV250_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP8769261.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(13703..13927)
                     /locus_tag="IV250_002001"
     CDS             complement(13703..13927)
                     /locus_tag="IV250_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769262.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            complement(14031..15941)
                     /gene="ltrA"
                     /locus_tag="IV250_002002"
     CDS             complement(14031..15941)
                     /gene="ltrA"
                     /locus_tag="IV250_002002"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP8769263.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            complement(16687..16806)
                     /locus_tag="IV250_002003"
     CDS             complement(16687..16806)
                     /locus_tag="IV250_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAP8769264.1"
                     /translation="MGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKGK"
     gene            complement(16821..18005)
                     /locus_tag="IV250_002004"
     CDS             complement(16821..18005)
                     /locus_tag="IV250_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP8769265.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(18264..18554)
                     /locus_tag="IV250_002005"
     CDS             complement(18264..18554)
                     /locus_tag="IV250_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769266.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            complement(18544..19686)
                     /gene="dcm"
                     /locus_tag="IV250_002006"
                     /pseudo
     CDS             complement(18544..19686)
                     /gene="dcm"
                     /locus_tag="IV250_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
BASE COUNT         5838 a         4031 c         3502 g         6381 t
ORIGIN      
        1 tttaaaggca gtaacaggat taaacattga aacataatcg ttactaaatg aaatcaagaa
       61 atatactgta ttactcaatt cattgaaaat atttatcata atttttaata gaaaacacta
      121 ttaaagagaa aaaagagagg taaatcaaat gaaaaaatct ttaaaacttg catcagcagt
      181 tatattaatg tcccaactcc ttatagcaac acctttccat gtgttggcaa gcgaaaattc
      241 aacatacaaa attgcaaaag aaagaaccga gacgactgaa atgagtaatt ccgatgtaac
      301 agcagagagt agtgaacttt tgacaacttc atcaagtgaa agtagtacga cttcaaatgc
      361 cgagtcttca acaacttcat caagtgaaag tagtacgact tcaaatgccg agtcttcaac
      421 aacttcatca agtgaaagta gtacgacttc aaatgccgag tcttcaacaa cttcgtcaag
      481 tgaaagtagt acgatttcaa gtaccgaatc ttcaacaact tcaagtagta caagtgaaat
      541 aacttcttcg aaagtgacaa aaccaaacca tatagaatct tcaaaaaagg aagcgccaaa
      601 aacggaagga caaacaaaaa ctttttcgac accacagcaa atagaatcaa cagaatcttt
      661 ccaatcacaa ccaatactat ctggtaatga agaaattcat tttgacaaaa atgaatctgt
      721 agaaaatttt attatgaaaa ttggtgagtc tgcgagaaaa attggtcaga aatatgattt
      781 atatgcttct gttatgattg cacaagcgat cttagaatct gccagtggtc aaagccagtt
      841 agctcaggca ccgaattaca atttatttgg gataaaagga acttataatg gaaactttgt
      901 cattatggta acaaacgaag atttgggtaa tggaacgctt tatacgaccc aatcaaaatt
      961 tagagtgtat gaaaactatg aggaaagctt tgaagattat gccaaattat taactaaggg
     1021 aattagtgga aataaagatt tttatgcagg agcattaaaa gctaatagta aaacatatcg
     1081 agaagcaact aaattcctta caggacggta tgcaacagat acacaatact atttgaagct
     1141 aaatgaatta attaaaacct acgatttaac caactatgat aaagaagtta aaggtccaca
     1201 aatgagtaag actggatacc aagtgcctat tcataattat tcaataacca gtttgtttgg
     1261 aaatcgtgga aatgaatttc accgaggact tgatatggca gctacacagg gtgagccgat
     1321 tcatgcagtt aaagctggta aggttgttaa agcagagttt cactactctt ggggaaatta
     1381 cgtggtgata caacacgaag atggatcaac cgcgttatat gctcaccagc aacaatacct
     1441 agtaaaaact ggagataatg ttttgcaagg acaaataatt ggctatgtag gaagtactgg
     1501 gaatagtact ggcccacacc ttcatttgga aatttgcaaa gatagtagtc tttctcagag
     1561 catgttagtt gatccgaaaa ccttattatt cggagactaa aaaaggtaat caatctgaca
     1621 aagtaacaag aaaatggttg gattcttaga cctttgatca ggatattgag actggaattg
     1681 atatctcgat tacgatgggt ttgacaaata aaacaagtcc attatcgaat atcgagtgta
     1741 ttattgccgt ctttatgctc atattttgag caggtttcac tagatgaaaa caatttatct
     1801 atcttgagga tttcgtgtac gtatcgttca gtcttgtatt gtaaattagt cataaaatca
     1861 gactaagaaa aattggaaat atttttttct gatttattaa aagttaattg aaattatatg
     1921 gtacttcttt cttgggaggg taggtgcagt gaaggaaaga tatggtcacc ttactgttgt
     1981 tgaggagatt actcataatg gatataaaaa atatcgttgc ctatgtgact gtgggaatga
     2041 aacgattgta tatcgatcaa acttactaac aggtagaacc aaaagttgtg gatgtggaaa
     2101 ttataaaaat aggcataaat ataaaaattt gacaaataaa acttttggta agttagttgc
     2161 aatcaagcca actaaactgc gaagtgaagg aactgttgtt tgggaatgtc aatgtctgtg
     2221 tgggagaaaa aagttagtat cggcaaagcg acttctaaaa ggtgatgtaa aaagctgtgg
     2281 atgtttgaaa tcaaaaaagc atcacattga aaataaaaaa ttcggattac ttactgcaaa
     2341 gtatcctttg gacaataact gttcatggaa aacaaaatgg gtttgtcatt gtgagtgtgg
     2401 cagtatgtgt gagataagtt atagtaactt attttttgga catactcaaa gttgtggatg
     2461 tttaaaaagg caagaaacaa ggacatgtgt tgaagggaca gtaatagagt gtctaacgtc
     2521 aaaattaccg aaaaataata caagtggtgt taagggagtt tatcgttcta aagagaaata
     2581 gattgcgtat ataactttaa aaaaaaagag gtactattta ggatcgtatg attctattaa
     2641 gagtgcagct caagctagaa aagatgctga aaataaattg tttcaacctt tgattgacaa
     2701 atatcgaggt aatcattaat tattgtggat aataattaac agtgatatat aaacatctat
     2761 aatatattat tcatactatt gatatttagt ataatcctat ggaattgaat atcatagcaa
     2821 tgtatttgaa aagcaaattg gaaaacaaat aggtgcaaca ccataaatat gaaaaaattg
     2881 ggaaaaagta gaatggaaat ttgcttacgt ctcacgagat agctcacact attaatctga
     2941 agaacaatga agtcgacttt tagaaattgc tagaagaaag taaagcgaat tatcactagt
     3001 tactatagag taagttcaaa taaagcatct ccaacttaca tggttacatg aggatatcaa
     3061 atcgaaatta ctcaagcttc ctatggctag agtcgcgaga agttgttggg cagtagacat
     3121 aggttatatt gttcttagct acacgatcat tagaaaggaa atcctattat gtatagttat
     3181 caagaagatg caaaagtcca aaaaaatcgc tggtggattt tgattgctat atctatgttt
     3241 accttcatgt ccacattgga tggcagtatt gtcaatattg cactacctac catctcagat
     3301 gatatgggca taccaatgaa tcaagcggaa tgggtagtgt ccgtttatct gatgattgtc
     3361 tgttcttgtt tgttgctgtt tggtaaaatt ggcgatagcc gtgggaaaat caaagtattt
     3421 cggattggta cgctgatctt tgtcttggga tcgctgttgt gtgggttcaa tcattcgcta
     3481 ggtattttat tgttcgcacg ggtggtacaa gccattggtg ctagtatgac tatggctaca
     3541 aatgccggaa tcatcacgga agtctttccc atacatgaac gtggtcgggc attgggagca
     3601 ataggtgcgt ttgtttcatt aggctcgatt gctggaccag gtcttggcgg cttgattttg
     3661 tcccaatttt cttgggcgta cattttttgg atcaatgtac cggtgggaat cgtaacgatg
     3721 ttgattgctg aaaaattctt accaaaagat atcaccatga gcggccatac aatcgatgga
     3781 gggggctttg ctgccattgc ggctacgatt atcagctttt ttggagcaat ctttttgggg
     3841 caagaaagtg gctttttcca tccagtcccc cttgtactgt tcgtaatttc agtgattgcc
     3901 tttgtcagct ttttaaaagt tgaaaagaaa gcagcctctc cattgatttc tttcggtatt
     3961 tttaagaaca agatttttac catgagcttg attacagcct tgttgatttt ttcttccaac
     4021 ttctttgtca atgtagtgat tcctttttat ctgcaaaatg ctcgcgggtt gagtcctagc
     4081 tatgctgggc tgttgatgat ggtctttcct ttgatgatgg tcgtcggggc gcctttaagc
     4141 ggctacctga ccgaccgggt tggcccggag attttggtat tagtaggctt gggcttattg
     4201 tcggtaaccc aggtcatgta tatgtttttg tcagtggact ctcctttgtg gctatacatt
     4261 gtcgctactg ccatcatggg tgccggcaat gccttgttcc agtcccccaa taatacgatg
     4321 gttatgagct cggtcactat cgaaaacttg ggactggcag gctcgatgaa ttcctttgcc
     4381 cgaaatttgg ggatggtcag cgggatttcg ctttctacga cggtcttata tcaagcgatg
     4441 tctgctaaga tgggccagca agtaactaac tatatcgcaa atcggccgga tgtcttttta
     4501 tatggaatga agattacctt tttaggcagc tttgttttgt gttttgttgc ctttttcctg
     4561 actatctggc gtatcaaaaa acaaggaggt attcagaaaa atgtctgaaa aaaccactgt
     4621 tgaagaattt ttggaggcct gcgtatgggt gagtgggatt tccatggatg atggaagatg
     4681 aagtgaactt ctaaaagccg aaaaactcaa gtattatctt caaaatacta gtgctcaatg
     4741 agatgcttga tttgatcaca agaagactgc gaaagccgct ggcaaccgca agctaggtat
     4801 cccaccgatg gactacgttt tagaggctac tggttatgaa tacggagcca acgctctcat
     4861 ctgcataaga atacatcacc cagattatgt aaagattttc gatacttctg tcaaaaaatc
     4921 attaagatag ataaatgatt tccgtctagt caaattggtt cagaatgtag acacacagat
     4981 ggtaagaagt cactcacaat tcgagaatgg acttgtccta tttgccgtat acatcataat
     5041 cgaaacgtca acgatagtat caatattttg accaagggct atgaatacaa gcattgcctt
     5101 agaaaacatc aagaacctta ggaacaacgg ggatagcttg gttaataaga gataccgctg
     5161 tttaggcttg caagtatact cttttatcaa gaattccgtg gctttagtca tgagaggctt
     5221 aatactatga aaattttctt tagcattttt gatttaggtt ccttttctaa ttataataag
     5281 atgaccacta cagtaaccta ctgaaatata taaacagcca ctgtagtacc attttcaaag
     5341 tagtctatga gaggagaatc ttcattctta tgaacgctgg aattgcggat atgaatttaa
     5401 taaaaaaaac ccttaatgat tttacttcca attcaatatc aaaaggtact ggtatcaatt
     5461 taagcacaat aaaaaagctg aaaagtggtg aaagatcagt ggaaaagctg aatcttttag
     5521 atgctataaa aataacggaa tttgctatga aaaatggaaa agctgagatt gaaatatgga
     5581 gataattatc gtctagtgaa ttacttgcca cttagctgaa tttaacgatc cttggtgctt
     5641 gaagggggag attcttggga atagagcata cttgcaagcc tatgtcttta ctaacaacga
     5701 tatctcttat taaccaagct atccccgtag ttctaaggct tttactttta tctaagcaat
     5761 aatcgtattc aaaggaaatt tttgaccctt agaaagtctc attttatcag acgtgagctt
     5821 aggcattgct gaattttgtt aattcggttt taggttgtgc tttgagcata atatgaaagt
     5881 attctttatc acgatttcat tcatctaagg taatgtgatg tgattctgaa attctttcaa
     5941 aggcagtttt tgcataagct gacattacat catcaatgac atgtttacaa tgtttcacta
     6001 ctaaaataag ggggtaacca agaagaaaaa ctgaatgatt atttgtatct aattccatgg
     6061 tatatcaacc atttcctata tagactgatt atgacatgga ttaaaataaa aaggcactat
     6121 gtcttaattg tcggttttcg agttaccgaa tggcattcat cacccaccta tagaggatgg
     6181 gcgacttctg cctgaaataa gttacactac ttataacttc taataaaatt agattgagtc
     6241 attattattt aacatcaacg attttccagt ttccattttt ttgtaatact aatttgtact
     6301 gaaagtagct agtggttttt gatacattat caaggtattt tatctttgta ctcactagaa
     6361 aattttttcc gttttgtaga taaactggat tctctaactc cgaaaacttt aagtcaatat
     6421 tgattggtcg catcgcacca ttttcaacat aatactccaa ttctttttca gaagctgatg
     6481 gataaagttt aaaaaatgtt tctaaaaaat cggtgatttc ttccgttgtt ttggcatcaa
     6541 tttctgaatc gttttctact tgttttgctt catattttgc cttgtctggc tttccaatca
     6601 ttgttggcaa actagttact aaatgattcc tgttttcatc ttcaaagatt gtcactcgat
     6661 atgcagaaga aattgattgc gtcttttttc cctctgtaat atgttgctcc actaaaaatg
     6721 aaacgacaaa ttcttccgag tgtttttcaa catctagaat ttttacagat tgaacttcgg
     6781 aactcgtggg aatatctgct cgaaccatat cttggattaa agcgagaccg ttttctgtaa
     6841 ggtactgttc gagattactc attctctttt caattgcttc tttgttattt ttccatgaaa
     6901 aatattcttt cgcaaaatcc tctgtaaaac tttcaacgcc agaagtattc actaccttct
     6961 cttttataac cactttttca tgagttgtat gttgatcaat ggcggtgaaa tttttataaa
     7021 cgccaaaagc aagactacaa gacaataaaa gccaaagggc aagcaccatt tttcgatgct
     7081 tacccacgga tacaactcgt tcttttttct tttttgggct cttttccttt tgattccgtt
     7141 caatttttat cttcattctt tttcctcttt tctcttattt gtatcggcca gctccgataa
     7201 gatgctgccg ccaatagttt tccgttagat tcgtccaccc cactggatct cctgcatgaa
     7261 acatgcgatt ctcccctgcg taaatgccca catgggtcac ataagtaccg gcatcatagg
     7321 ttgaatgaaa gaaaactaaa tcacctggtt tggcatcttt tagatcaata tgagtcatgg
     7381 catcatattg atcctgagcc acccgaggaa gtttcaggtc aactttggca taacaccatt
     7441 gggtcaagcc actacaatca aaccccgttt ctggacttga gccaccaaac acataggccg
     7501 ttccttcata cttaaatgcc tcatcaaaaa tttcttggac tattttgtca tcaaactgag
     7561 ttgtagttag gtattgcttc actaataaac agtaaaacat attgccataa ttataccgcc
     7621 acccaccatt ttccttaatc gcgattgggt tggggtaatc gactttttga ccacctgatt
     7681 tttcctttga aaaagattca gctaaagcaa aactatgctt cttcccgttt ttcccgacat
     7741 gatctagata cccgccacca tagttatagg cctgaataat actatcttga tcacacccta
     7801 gttctttgga acgcttaacc aaagacgcaa aatagagaca gccttgtttg atggatgcct
     7861 cggtacttaa tgaattaggt ggtaatccgg ctgactcaga actttgcata acgtcttcca
     7921 ttgtgccacc agattccact tgaataatcg ccaaaagaat tggaatatac tctgttatat
     7981 ccgattcttt ggcatacttt tccaccattg cacggtgttt caatacttct tctgagacac
     8041 tagttccgcc aggagaagtg ccagaactac catttccctc actgtcttca ccaaacaaca
     8101 aacccacaca catcaaaata cccataaaag agatggtcag aaaagttaat gaaataacac
     8161 ccactttctt caagttcatt ttttctcacc tgaccttgat tttggacgat ggttcattct
     8221 tttttgtact ttttgattgg tctgctttgg cagagtggac ctctgtttca cttgttggaa
     8281 agaatttttc ttacttgctg attggctttt cccttttgaa atgatttctt gactagttag
     8341 ttttcgtttg atttcgggat tcagatttgc tctcacaatc cgctttggtg cttctttttt
     8401 cagcatcata tctttctgag gttgagctgc tttatctatt ggaatattcg cccttctttt
     8461 tccctgacga tttgttgctt cactcagtat cttgcgtttt tcgtccattt tcgcatttct
     8521 tcgttgtcgt tgtttttctc gcaaatccac tcgatccgct ttttcttgga acagtccgcg
     8581 cttgaattct tctggtgctt ttttcgactt ttcgattcct ttatggagat tgtatttcaa
     8641 attcgttggc gtattcctta cctgatcctt tacttgttta gtcttatctt tcaccaaagc
     8701 ttgggtatcc aaaactttgc cgatttttct cccagccaaa ttcattcggg attgtttctt
     8761 attatttgaa actggattct ccttagttaa gtcagatagt atttcgtgat cgttcggtaa
     8821 tcgtgaattc tttcgtagag aagaaccgga tggcttaaat ttggattttg attgttcctt
     8881 tgccaactta tttccgactg ctgcaccacc accaaaagcc aaagctcgtc ccacatttcg
     8941 attgagttgc cgcaattttc gattcattaa catttgcggt tttcgcatga cgcgtcttcc
     9001 cattgactgg ctatcattac tctggaggtt gaacatactc ataatgtcgc ccaatttgaa
     9061 atagataccg gcaaaggtca caatttgcag aaaagcaacc atgaaaaatg gataggttgc
     9121 cgaaatattg aaaaacatcg tggagatgct aaatgcagtc gtaatcacca aagtaacacc
     9181 cgctcgcatc ataatcgtat tgaataaacg aataatcgcc ttctttccta aactttcata
     9241 agtcggcaac atggacaata agaaactaat tggcagaaac atcgcaaaga taatgaagag
     9301 aatttgcgag aacaacataa tccctgttag aagaaaaaca aagaaggaaa tgccaatatt
     9361 gaacaatacc aaaaagacca cggtccctaa acggttcatg gtcttggtaa tactgagatt
     9421 cgcattgtca ttgtcttcaa tctctgcctt gaccgcttct tcccgatctt tgcctttgtt
     9481 ttcatctgga ctcacagata gaatcttgtt gacccggtct tcaccaattt cttctatgtt
     9541 ggaatcatca aattgcagta gtaaccaagg ttgttggact tgaatcgaaa acaaactatc
     9601 ccgaatcaaa tccacactat cttttccttg gctttctgaa ttgggtacaa cgatctcggt
     9661 tccaagggta agggccgctt cactaacatc tgagctaaag tcattgattt ttgtgatata
     9721 ggtaggcgca taagcaatga acgaacccga tagtagaaaa atcatcacaa aattaatcac
     9781 ggctcgaact gctttcgtgg tttctcgttt taataatccg gtatacgcca catacacccc
     9841 cagagctaaa atcatcaata atagaaaccc aaaataaaat ccggaggtct ggagtccatt
     9901 ttcagtaata ccagccaatg tttggatatt ctttccaata ctttcagccg tatccgaaat
     9961 gaaatctaat ttgtaagcct cttgaaccac ataacccgtg gcgttcgaca aatacaaact
    10021 caccgtccaa atgaaattag taatggcata aagaccatac atcacgcttt tccctaagcc
    10081 atcaccccaa ttccaaggca accaatccca agagctatcc acaaagaaat ctagttgata
    10141 gttgttcagt gaatatttgg aatatagatt tcctgcttca actgtatcat caactagccc
    10201 tgtcgcctcg gctaccgtac caactaaact caatagaact atcagagtga gtatgctaat
    10261 aaaaacgatt cccacaatcc gaaaaagttt ctttttcatc ccctcactcc attctctctt
    10321 gaacaggtgg acgggtatca aaggcatgga agagttcttc aaagactgga tgtacctgaa
    10381 ccacgcctac ccgaccatac aaatcctgca tcaagcattg gccattttcc aaatctcgta
    10441 ggcgtttttg attgctttca tcttctcgat ccaagccaaa gaattccaag gttttcttga
    10501 tttcatgaat atcggtactg cgaaacgcaa acttcaaccc gatattattc ttcaagcgtt
    10561 catccagtaa atcatcacta ttttgcgtga caaagtaaac cccggcattc atagcccgcc
    10621 cagcacgaac gagcttattc gataatgttt tcccttgcgc tacttgaaga aaactccacg
    10681 cctcatctaa gtctacaatt ttgaagattt ctcgattact atggataaag tccaaggcaa
    10741 aagtggaaat gacgattagc atggaaacac taagcaattc catggtggta tattcttcaa
    10801 aagtggtttc tgcatctggt aaaaccaaat ccgctacttg aatgatattt aactgcctat
    10861 ctaaactaat cgactgggta atcgaaccat ctgagaaaag caaatgggca aaatcataat
    10921 cggtaaagga ctcgatgtga tccgcgatat tttccgcaac tggtgagcct tcttttcgaa
    10981 gctcattaat tacttgtaat aatccccgtt gattgctttg ggtaaccgtc cgaatggcct
    11041 tacgcaacac tggaaacttc tccccatctc gactagaaat tcctgttaaa aaggtcagaa
    11101 tatcaattgc tagactttca gaatccttct ttttctccat aatcacataa ggatcaagta
    11161 atccttgatt ttttggctta ctagttaagt tgagaatatt gatttcatcc gcaatttctg
    11221 gcaaggtttc tttccagcca ccccgttccc cttttggatc gacaatcaca gcttgtccac
    11281 caaaaaggac ggcgtaataa actaagagat tgttactaaa ggactttccg ccaccgagtg
    11341 aaccgaggaa tgccgctgct agcgcattgg taactgaatc cttgacccct tgtgaggcca
    11401 atgccggttt caggtaaaca ttgcgccccg tatctacgtt ataaccaaag taaatgcctt
    11461 ccaactcccc taacatctga gtggcgccaa aaccaagtcc ggcgagaaaa tcagacgtaa
    11521 catactggat ataatcattc atgtatcgct tactagaagg taagaattct tcatgtagcc
    11581 ccatcatatc gccaaaaggc cggactagtt ttacattggt atcatcataa aaatctagca
    11641 cttcatcaca ccggcgcttt agttcatcta ctgattcggc acttactcga accacatagc
    11701 tcagtttgta catggattct ttggactgat ccaaggtagt ttccaattca tctacggaat
    11761 ccaatgcatc taagacattg gaatttgttt cattgtcaga ttgataggca tggttgtcca
    11821 agtcttttaa ttcctttttc ttattgcgaa cggtagcaag tgcctttttg ttcgtcacaa
    11881 tctccacatt catactagtg tcaattggga atgtaaattg ttgttgctgg taatagaaaa
    11941 gttctgagga gggaaactcc atttccccca caattgtatt aattgttaag tacgctacat
    12001 atgattcatg attctcatgt tccatgcata agtaccgggg cttttcttca atcaaacaac
    12061 gactgggacg taataagtcg taacgtttca ccaaagtttc cgacttcagc tttttctttg
    12121 gtagctgaaa ttcatattct tcaaatggtg tacccttttc gccatagata tgttcaatca
    12181 aatacatcag atcactcggt gtgactcgtc tgactttaaa tcttcgagcc aatttacttt
    12241 ccattagttt ttcaagcttg gtatatcggc gaatttcttc attggataag gaaacaaaat
    12301 cgcccatcaa atgatgattc actccataga caaattcttg aaagccggag aagaaagatt
    12361 tcttgagatt ctttagattg acctcttcat ctgtcgcaat cagtttgaag ccaataaaaa
    12421 agcgataatc aatctgacta tcgccaatca tggatactaa tgcctctgtc tgtaaatcaa
    12481 ttcgttgttt cgctacttct gttaatcgcc cggtaatttc cttttttgat cgttcttgcg
    12541 tggcacgaat actactttct gtggcaattt gtaaggcatg aatcttgcct tcacgatttt
    12601 gcgcaatcaa ttgacgaaag ttatcgtgga cttgatattt ctgttcaggt gacaaaaagg
    12661 agtaattgta aggtattaac tcatagtagg caaaacattc tccctcttga ttaaagacaa
    12721 gattattttc aatatactta atcgggtaca tagttcacac tccttaccag agtaatgggt
    12781 tcgtcccatc gtttcttctg ttctttgact ttctttcctg cgaaagtgac cttaaatcgt
    12841 aagtgatagc taataaaaga tcgtaaaaat cccaatggtt tcttaccgtc aaacgttttt
    12901 tgcgacatga accaagtaac tgccaccggt atacctaagt attttaaaaa ggccccctct
    12961 atcatagata gtggaggcac atgggcaaat acgatgacaa acagcaagga taaaacaaac
    13021 caagccatct ggttaaaagt cacaggaaaa ggcagttgaa agtcattaat tgcataaatg
    13081 accttttcaa ctgcccaaat actggtgtag ctttttattt tcttcatcaa atcattcctt
    13141 taaaaagaag gatagtttag aaatcaacta cccttcaata attttgttaa tatggatact
    13201 caaaaacacc tcgactcgtt accaaataat tgcctccaat ctccaagtct ctaccacacg
    13261 catcaaaatc aatatacgtc ttcaagtgtt ctggtacttc tccaaaatca ccagcttcta
    13321 caatcaagaa atatgctaca tcactcatat catcgcaatc tggataatag ataatatcat
    13381 ccacatgctc taccatctcc tcaaaagaat tgaagaaagc atgttgaatc tcagaagcaa
    13441 cttcaccaac tggcgttcct tcaagttcct ccgctaagcc acacaaccga ttaatttcct
    13501 caattggcgt atactcatca atctcaaagg gaagttcata atcatgaatt gcatattcct
    13561 cgtattcgtc atttaaccca attcgttctt tcacttcatc aaaatcgaca ggtggggtaa
    13621 accacgcccc caccaattca ccttcgttat atttcccgag gttcgcaata taaacacgca
    13681 tctgttccat cgtgcttccc ccttacgcac cgacaattcg gttaaacaaa ctgagtaaga
    13741 catccttcac cccagaagca ttaaagacca atccgaccgc aatcaaagca atcaccaaaa
    13801 agccaatcag tttggaaaac tcccgcttaa atcctaaaaa caaaccaatg accacaattg
    13861 ccagtaagac taaggattgg gcattggtta aaaaccattg atatatggat aggtaagttc
    13921 ctgatattat aaatatctca aacttttccc cccattcaca cggagcgtgc gactttcacc
    13981 gcactccgcg ttccatcgtg tttatattga aaagtattta tgtagtagac ctatatcaag
    14041 tttaattctt gattgccagc taatactcta tatttattta gtcttatgag ctggacttta
    14101 tttagcttca atagagctat atatttttgt attgtttctt ctgttttagc gtgtattaga
    14161 cggtgtaccg cctcttttac caaaataaga tttttataat cgtcatttcc accagctcca
    14221 gttggtacaa tatgatgaca atgtatttca ttagcttgtt gaaattcttc cagagttata
    14281 gcacattttc catgttgagc agaaaatagc gaaattcgat tgtctgcgta ttctattgtg
    14341 cgattttcca aagataattt cattaactgg tacatcagta atttatcaac acttagattg
    14401 gtgtggataa attttcttcc ctctagagtg tagcaattcg ctaaatacac ctttgccatt
    14461 ggttttctgt gttggatata tccaacaggg aaaataggtt cgccctcgcc agcaacatac
    14521 cgaagcattt tagatttacc atatcttgtt gcctcctttg tcgtaagttg tctgcctgtt
    14581 tttctcaatc tgcccctttt gtttgtacct aatctgttag tgaagatagc cattacagct
    14641 ctattcagtt tcatacaatc cagatttaca ttcgttgcaa tctgataata attttgcata
    14701 cctaatacca ttgaattata aagtcttgtt tcttctaatt ccgttttccc ttttgaggga
    14761 gaagcaattt tctttgcctg tgataccagc ttgtcttttt ggtttttgaa ttgtttatca
    14821 cttatatgtg atttaacaac taacttcttt cttctcggta tcattttaat cttgaaacca
    14881 agaaagtcgg agtatctttt tctaacattt acaattcttg ttttttcttg tgatacttcc
    14941 aattttaatc gttgttcaat ccattgagtg attgctattt tggctttttc agcactttcc
    15001 ttgtagcggc agaaaactct aaaatcatct gcataacgaa ccatatacat ttcctttaac
    15061 tttgttttct tcatttcttt gtaagcgttg cttttacatg ggctaccgct tttattgata
    15121 tgaaccacat agttttttgt aacaggattt tcctgccatt ggctttctac ccaatggtct
    15181 aactcattaa gaacaatgtt tgctagtagt ggggatatta ttcccccttg cggagttcct
    15241 ttctctggat atgttattgt accgttagtc attttgattg gagcttttaa tatccttttt
    15301 aaaatgaata tcaagtgctt gtcccgtatt cccatagccc atatctgttt tatcagcttg
    15361 gaatggttca cgttgtcaaa gaaaccctta atatcaaatt caatcacata atgtagctgg
    15421 ctgatttgta ggcggttata ggttgctttt attgcatttt caactgacct attaggtcta
    15481 aaaccatagc tgttttcact aaatttagct tcgcagattg gttctaatac ttgttttata
    15541 cattgttgta taagcctgtc ccacatacaa ggaataccta gaggtctggt tttaccattt
    15601 ggttttggta tttcttttcg cctcactggc ttaggtcgat acccatgtgg actgcctgca
    15661 acaatgtatc ttactttttt gacaactgtt tctgctggca atttgccaat atctttaatt
    15721 attgtaccgt ctgttcctgc tgttttactg cctgcatttg ctttgatatt tcgatatgcc
    15781 agtaatatat tttctctcga taggataata tccataagat gagtgaaaat atctccattc
    15841 ttacttttgg catataaatc atcaaaggtg ttttgcattt cgtaatattc agcgtgtctt
    15901 aaatcttcta cacacaatgt ttcattttta tttttaggca taaggcttca ctcctttact
    15961 tgaaattgag tgagcttttt tatcttactc gtaatcctta ttgaaacaac ataatacttt
    16021 tctaatatat acacgacttg agggcattcc tccattctca ttacaagaat ttcttaggtt
    16081 cgccctctgc ttttcagcat tagttcaact gcttattgtt cttcgtcagt ttattctctt
    16141 gatgagaacc caataccttt cctcgttccg ataatcttat ctatacatat atatccttag
    16201 gtcactagct ataagcctgc tggcttgata ccgcctgtaa cgttatatgg catttcataa
    16261 cctatattct tactttgcca caccagctac gatttctcgt acctttgcat ttctacaaag
    16321 ttcaaattta gacccgtaca ttcgctgttt cgtcagttcc ttatagaaca ttctaaccat
    16381 agatatttta tagacccccg acctgttgga tacgctaccc acctctggat agttttcgat
    16441 agaagatata tgttcatcaa ctattacggt atctttcagc cgtctttacc gagcttcaaa
    16501 cattctgagt ttcctcatac aatgcttgtc ggaggctcag ggcaggcgtt tcagagcgtt
    16561 acttcatcat tccacctgtc agattatcag ttctcctaat aaccaaactt tttcgttctt
    16621 ttattagtgc cttagctttt cactaaggaa cgagtcgcac ataaattttg tccgaaattc
    16681 atcttgtcac tttcctttct tttccattgc tcgatccgct gctgcacttg cattcatcaa
    16741 gcacatcaca gtaacaccaa gcatactgcc accaaatacc aaaaaaatat cttttacaat
    16801 tcccatgacg attcctcttt tcatataatc aaattttcaa ttgccatact ttgttgctcc
    16861 aaaatctttt tatgtcggtc tgataagcgt gcattctgta ccatatcctg gatataggtt
    16921 gtccgattca atcgatccaa ctccttagcc attttccacg taggggcgac ttgtcggcct
    16981 agccaattca aggttcgttc aatggtatac ggttttggct cggaagttaa ttgaatctct
    17041 ttccgattcc gtccaataaa acgcccccat tgttcattca agggccagtt ggttcgcctt
    17101 ttaccttcta ctttatccgc aaatcgcaag taacgattga taatggaaaa cgtggtgctc
    17161 tcaatgctac gaccttttaa caaatcttga atcgcatgaa aagcacggtc atttttcagt
    17221 cgaatctcaa aacgattttt cgtctcagtt tggtctaatg gaatccctaa cttgatatat
    17281 tgttcatagt ctttttcata caaacagaaa taaacctcac tcttgagact gccgatatat
    17341 aaggtattgc ccatgccgtc cttttcatca gcctttaaaa gttccccgga acgataactt
    17401 ttaaaggtac gaaacaagga gatacattct tccttctgac attttttcgt tagctcaggt
    17461 atatccaata gccccactcg atcattgatt gccaagtcca agcgtttcat cacgccaccg
    17521 gcctttagac aatcctcgaa aaagtcgtac cagctacgct tttgagcgag taaaaaagtt
    17581 tcaaattgtc ggcaacctcg cccctttaat tctaaaagca cacctttatc ttcttcatga
    17641 gacaacatca ccacaatatc acccatgaca tactgttctt gataggagta aaaggcataa
    17701 tcttcatgga gcatatactc aaatttcaaa tggagaattt cttgaataat ttttcgtgca
    17761 tccgttgtgg gaaagcgaat ccttacatag tcaaacagta acgtcaaagg caaatcggga
    17821 ttaaagcttt ccagttggtc aaaaattttc ctcatggttt ttatagatgc ggttacgcca
    17881 ccattttcta tctgatttaa gtacggccga ctaagtccac aagccgtagc caatttattt
    17941 tgtgaaacgc cgtattcgtt gcgtcggtct tttaatagcc gataatctaa attccgttgt
    18001 gccaagttct cacctccaaa ttcaaggtgt taactttttc aaatgagtta acacctatac
    18061 aaattactta ctatctttga tacaacaagc attcttaatg gttgcttact tattttttgt
    18121 gtttttgtac ccccctgtta gatacggggg tgagacatca tggtcggcta acgccgtcca
    18181 tgaacaggca cagcaccacg gaggatttct gcttcgcgaa tcctccgcgg tcagcgccta
    18241 acagtcagtt gtgttggttt aggttactca tctgtaatac catttacttc caactctgct
    18301 ttcatttcta acaagtattg acgaatatct ggttcttttg cttggtccgc aatccgctca
    18361 aattctgcaa ttacttgatt tatttcaatt cgagcatccg tctcctcatt cattaaatat
    18421 ggatcgccag cgactttgac accattcttg cgaatatcaa attttaaccg tcgcaattgt
    18481 tcttctaatt ctgggtctga ctgaatcttt ccaatcaccc aataaatttc tccaatcaac
    18541 tcatcataaa ccatgtacat ccaactcttt taatcttctg gcaatatcaa aaatgaccgg
    18601 aactgttaca gagtttccgg cttgcttata caactgacta tctgaattga cttgagaatc
    18661 tcgatcaaaa gcccaatcgg gaaagccctg aagtcgccaa cactctctag gtgttagctt
    18721 ccgaatttga tactgatccg tgacgatcgc ttgttggtct cctgttagta aggtatgaac
    18781 actctgtttt cccagacgac ctcggcgact tgtggaatta ggaaaagcca gattcacact
    18841 gtcttctggc ttcacctctt gatattctgt ttcatttttt cgaatcatga tttttgtacc
    18901 accttgtttc gttgacaagg tgggggcggt tccagtaact tcataaactc gattaccttg
    18961 ttcaaacttt cccggtaata gtcctgctac agagattcca tcccttgctg gcagtgcgat
    19021 tttgattcct tcatcacctg ttaaggttgg cgctacgcca tctgaaccat agacttgtcc
    19081 acccattcct cgattggaag gattgatatt taccaaattt ctgatgtttt gatcagatgt
    19141 tgaagacggt ccaatgatag gaaatacttg ttcggtacat gcgtctcgag aatgtccgat
    19201 aaggaatatc cgctcccggc tttggggaat gtaggccttt gagttaagca cctaccactc
    19261 cacgtcatac cccaatccat ccagcgttcg gaggatcgtc tcgaacgtag cccctccttc
    19321 gtgattgagc aatcctcgga cgttctcaag gaataaaagc ttaggtcgga gaagagtggt
    19381 gaaccgagcg atttcaaaga ataaagtacc tcgagtatcg gaaaatcttt gccgctttcc
    19441 cgcaattgaa aaagcttggc acggaaatcc gccgcaaagg atgtccactg gtccgaggga
    19501 ttgaataaac tcatctgata cacttgtgat gtcatgcatt tccacctcct tacttgtatc
    19561 atgaattgct ttataactac gtctagcaaa tttatcaatt tcacaaaaac cgatacaatg
    19621 atggctcgcc tgttccatac ctaaacgaaa gccaccaata ccggcgaata aatctaaaaa
    19681 tctcatacat tacctcattc attgcaaaga aaaaagccaa gaacattttg ctcttggctc
    19741 atacattatt aa
//
LOCUS       DACDIE010000050        18578 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.050, whole genome shotgun sequence.
ACCESSION   DACDIE010000050 DACDIE010000000
VERSION     DACDIE010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.050"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            6..305
                     /locus_tag="IV250_002007"
     CDS             6..305
                     /locus_tag="IV250_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8769267.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="IV250_002008"
     CDS             378..1739
                     /locus_tag="IV250_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAP8769268.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="IV250_002009"
     CDS             1761..2021
                     /locus_tag="IV250_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769269.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="IV250_002010"
     CDS             2035..2787
                     /locus_tag="IV250_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAP8769270.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="IV250_002011"
     CDS             complement(2881..3486)
                     /locus_tag="IV250_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8769271.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="IV250_002012"
     CDS             complement(3539..4513)
                     /locus_tag="IV250_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HAP8769272.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5670)
                     /locus_tag="IV250_002013"
     CDS             complement(4891..5670)
                     /locus_tag="IV250_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8769273.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPLDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IV250_002014"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IV250_002014"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP8769274.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="IV250_002015"
     CDS             7161..8558
                     /locus_tag="IV250_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8769275.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="IV250_002016"
     CDS             8639..9079
                     /locus_tag="IV250_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP8769276.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="IV250_002017"
     CDS             9227..9553
                     /locus_tag="IV250_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP8769277.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="IV250_002018"
     CDS             9554..10303
                     /locus_tag="IV250_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769278.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="IV250_002019"
     CDS             complement(10383..10838)
                     /locus_tag="IV250_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP8769279.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="IV250_002020"
     CDS             complement(11265..11540)
                     /locus_tag="IV250_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769280.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="IV250_002021"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="IV250_002021"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAP8769281.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(12978..14429)
                     /gene="cls"
                     /locus_tag="IV250_002022"
     CDS             complement(12978..14429)
                     /gene="cls"
                     /locus_tag="IV250_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP8769282.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            14770..17436
                     /locus_tag="IV250_002023"
     CDS             14770..17436
                     /locus_tag="IV250_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP8769283.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            17800..18552
                     /locus_tag="IV250_002024"
     CDS             17800..18552
                     /locus_tag="IV250_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8769284.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
BASE COUNT         5970 a         3212 c         3832 g         5564 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag ttgccctgca tcaagtaata agaacagcca agtaaaaaca gcttcttatg
    12721 tttttaactt ggttggactt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    12781 aggtcgtgag cctgccgtcc agctccgagt cacaaggaac aagttcaaaa aacagcttct
    12841 catgtttgtt gaatattgtg acttgtgacc gaggagttgg caggagaaca ccgtttgttc
    12901 ggaagaaggg actgtgacaa gatttttgtc acagtccctt taatcttagt atatttttct
    12961 ctacttgaat aaaaaaatta taggattgga gaaaataatc gagaaatttg ttgcttgaat
    13021 aatagccagt tagacatttc cttgactgtt tcagttgtca tggatgaaca atgtgtctca
    13081 tcttctttga actgagtagc aagttcttct aaaaactcag gatcgtaaat gacagcactt
    13141 gtttcaaaat tcaatcggta gctacgaata tcttgatttg ctgaacctac catgcagatt
    13201 tcatcatcca ttatcaatgt ttttgcatgg aggaagccgt tctcatagac aaggatttgt
    13261 acattttcct tgatcagctg ccgagcataa tattgtgttg ctcgataaat aaaaggatga
    13321 tccggtttgt tgggaatcat gattttcaca tctacaccag aggcagttgc gatttttaaa
    13381 gcagcaatga cactttcatc aggaacaagg taaggtgtct gtatccaaac acgtttttta
    13441 gcagaagtaa tcaatttgat aaatgaaagc ttgatttgtt ccctttcgtt attcggtccg
    13501 cttgaaacaa gctggatgga tgtatcagca agcttagatt catctcgatc tgctttttta
    13561 aagaaataat tcaaatgata gccgaccttt ttttcttcag gtaccgagac gttccagtct
    13621 gttaagaagc gaagctggag caaagaagaa gcggcaccga aaatccgtat atgcgtatcg
    13681 cgccaatagc cgaatttttt tgttgtattt acatattgat tggcaatatt gaaaccaccg
    13741 gtataactaa tctttccatc gatcacaacg atttttcggt gatcatggta attcaatcgg
    13801 aaacgaagta atgccctttg tgaggtaaca aatgtatgga catgtccacc atttttgatt
    13861 agacgattga aatcttttgc ttttgtgcca tgagagccaa atgcatcata taatattcga
    13921 acttccacgc cttctgcggc tttttcttct aaaacatgta agacttgctg gccgatatta
    13981 tctgtcacaa atgcgtaata ttcgatatgg atcgaatgtt tggctttttt tagatcttga
    14041 agcagtgaat ccaatttctc ttgtccgtct gtgagaagag tgacagaatt cattcttgtc
    14101 agcggcatac gatttaatga agagaagaag tcaacaaatt gctgtttgtc tttatcgccc
    14161 atgtcaggat cgtaatgttc aatggtatct cctctaaaag actgaaagtt ttctaattct
    14221 ttcaagtcac tttgtcggag atagaatttt ttcttatccg ttaatccacg tccgaaaaat
    14281 aaatataata caaagcccac cccaggaagg gcaaacaata ctaagagcca tgcccaaatg
    14341 gctgctacat ctcggggttt gatcaaaata gccaccaaag caataagtgc atttaataga
    14401 taaagggcgg ttataatact agataccaca aaaatgttcc tccttacata caataacatt
    14461 agtatagcat gatagaagaa agaagcctac caaggtgaat atacttttca ctcctttcat
    14521 gaaaatgtag gtaaatcggg ctaaagtagt tgctttttgt aggttaataa ggtaggatat
    14581 tagtacagtt gaatatctcc ttaggggagt aaccggcaac gtttgttgcg ataatatcaa
    14641 cagtaagtta accaaatggt tttctggtat tgtatgaaat ggtgagacct aagacaaatg
    14701 cgtatttgtc ttaggtctct tttttttcag acaggactag ggccaagatc aaaaaatggg
    14761 ggatgaaatt tggaaaacaa ggaacaagca caacaagcgg tctataaaga aacgatcgac
    14821 tcgttgatgc agcaaacgca gagctcaacc gatggattga gcaccacgca agcgaaggaa
    14881 aaattagaaa gagatggctt gaaccagcta aaagaaaagc cgaagaagcc aacttggaaa
    14941 ttgttcttag aaactttcaa agatgcaatg gttatcgtat tgctgattgt cgcactgatt
    15001 cagatggtca tgggtgacta catcgaattc ttagttattt ttgcagtatt gatgttgaat
    15061 tctataatca gtgttgtcca aacaaaaaaa gcagaaggtt ctttagaagc gttgaaaaac
    15121 ctttcagcgc ccgatgctcg agtgattcgt gatggtaagg aaaaaacgat tccggcaaac
    15181 gaacttgtgg taggagatat cgtgttgtta gacgcaggcg attatgtacc tgctgatgga
    15241 cgtttactag atgcgggatc attaaaagta gatgaaggta tgctgacagg agagtcgact
    15301 tctgcagaaa aagaagtaac ggatatcaca agtacggtac caatcggaga tcgtacaaat
    15361 atggtattca gtggaacgat cgtaacttac ggacgcggta aattcttagt gacttcaact
    15421 gggaataata cggagatcgg acaagttgct ggccttttgg aatcaacaac tgagcaagtc
    15481 actccgttac aacgaggtct ggataaattc agtaaaaaac taagtttggc tattttggca
    15541 ttgtctttat tgattttagg tatccaactg tttcgtattt atcttggaga aggtacagga
    15601 gatatgactg cagatatcgt cagcgcagtg atgtttgccg tagccgtagc cgtagctgct
    15661 attccagaag cattacagtc tatcgtaaca atcgtgttat ctttgggaac gaataaaatg
    15721 gcaaaacgtc atgccattat tcggaagctt ccagcagttg aaacacttgg ttcgacaagt
    15781 atcatctgta cagataagac tggtacattg acacaaaaca aaatgacagt tgttgactac
    15841 tttttaggta atggcgatac tggggatttc acaaatgacc cttcaaaatg gacagctgac
    15901 gaacgtcgct tgatcgaaat cagtgtttta gcgaatgatg catcgatcag tgaagacggt
    15961 actaaattgg gcgaccctac ggaagtagcg tttatcgact ttagtgaaaa attaaaccag
    16021 ccttatcagg aaatccgaaa caactaccca agacaagcag aacttccttt tgactcagat
    16081 cgaaaattaa tgtctactgg acacacaatt gataacaaaa attacttgtt tgttaaaggc
    16141 ggaccagata tcgtcttcca acgcagtaaa aaagtattgc taaatggtga agtcgttccg
    16201 atgacggatg aattgatgaa aaaattccaa gagcaaaatg aagctttttc aacacgcgca
    16261 ttacgtgtac tagcttttgc ttaccgtcca cttgataatt ttgatttatc atttgaagat
    16321 gaagacgatt tgatccttgt tggtttgctt gctatgatcg atcctccacg tgctgaagtg
    16381 actcaggcgg taacagaagc aagaagtgca ggaatcaaaa caatcatgat tactggtgac
    16441 cacaaaacaa cagctgttgc tatcgcaaaa caaatcggga tttttgaaga aggaaacctg
    16501 gctttgaccg ggacggaact agatgaattg acagaagatc aactaaacga agttttagaa
    16561 aaagtcactg tttatgcacg tgtttcacct gagaataaaa ttcgtatcgt tcgtgcttgg
    16621 caaaataaag gtcatatctc agctatgaca ggtgatggtg taaatgacgc accagcattg
    16681 aaacaagcag acatcggaat tgctatggga acgggtacag atgtggcaaa agatgcagca
    16741 gcgatggtat tgacggatga taactttgct tctattgtca gcgcagtaga agtaggacgt
    16801 aatgtgtttg ataatatcaa gaaagctatt tcttatctat ttgcaggaaa tttaggtgcg
    16861 atcattgcga tcatctttgc cttagttgct gactggtcca atccatttac agcattacaa
    16921 ctgttgttca tcaatttagt caatgattca ctgccagcaa ttgcattagg tacagaaaaa
    16981 gctgaacctg ctacgatgaa ccgcccgcca agagatccaa attcaggaat ctttacggac
    17041 aaaacattga tttctgtaac gtatcgtggt gtgctgattg ggattgctgt tatctttgct
    17101 caatggcttg gcatgcagca ttcacctgaa ttcggtgtag ccatgtcatt ttcaacattg
    17161 atttggtgtc ggacattgca aacattccca gcacgttcaa acacacaaac agctatccaa
    17221 gcagggttct tctctaacag aagtgtctta ctagctgtag ttgtatgtag cgcgctttac
    17281 tgcttaacaa tgatcccagg actgaaagaa gtcttctcta tcccatcagc atttggattg
    17341 acagaagtct ttacatgtat gggaatcgca cttgtgacaa ttgttttgat ggaaatcact
    17401 aaattgatca ttgtacgagt acaaggaaat aaataaaacg aatcaacgag gttgtgagcc
    17461 tgccgtttaa ttctgagtca caaggtgcaa tttcaacaaa cagcttctca tgtttgttga
    17521 atattgtgac ttgtgaccga agagttggca ggagaacacc gtttaaggaa gaagttgtaa
    17581 aatcagcatt ttgagctaat actgagggtc atctgattgg acactgttta ttcggaaaga
    17641 gggactttga cacgactttg gtcacagtcc ctttttttat ttatgataga cggaaagcca
    17701 atcattttat tattcatcaa aagagataga atagataaaa gaggagaatg atttattccg
    17761 aaaagtgaaa acatataaca aaaaattaga ggaaatcaaa tgaatgaaaa agaatgggat
    17821 gaatttgctg aagaatatta tgaaatccag caagaatcgc aagtaactat tgctgaagat
    17881 gtaaaaaatt atctgaaaac ggagaaagtc ctgcctgctg aaacagcagc agacgttgct
    17941 ggaggatctg gcagatttct ttccttagca aaagaaatta atcgctatga tttgatcgat
    18001 ttttcttcag gaatgctaga aaatgcaaaa aaagaagcag aaaaaaatga gttgccaaat
    18061 gtttccttta tcaaacagtc gttagaagaa tttatccaat caaatgagac atatgaaatg
    18121 gtttttacag cagcaaatcc agcattggta catcttattc aattagaaaa attgatgaaa
    18181 aagtcaagca aatggtgcgt gataatcaga gtagtggaat caatcgatga tgttttttat
    18241 cctatcgaaa agcaactagg aatgatagaa gaagatccta atacatcgcc agttgtaatg
    18301 gatcaatttg aaacatactt aattgatgaa ggattcaaag tgaagaaaaa agattttact
    18361 tattttacac aggaatcaat tactgaagaa ttgatgaagc tatactatga cgcgtacgca
    18421 aaagatcctg ttgtgaccag gtacattcgc aaactattca gtgaaaaacg agaaatcctt
    18481 agtacgacta agctgaccta tagactgctc ctttggtccg cagataaaaa aagaaccttt
    18541 cacgactcgt gaagggttct ttttatgatt ttattata
//
LOCUS       DACDIE010000051        16617 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.051, whole genome shotgun sequence.
ACCESSION   DACDIE010000051 DACDIE010000000
VERSION     DACDIE010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16617
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.051"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..597)
                     /locus_tag="IV250_002025"
     CDS             complement(<1..597)
                     /locus_tag="IV250_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769285.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQR"
     gene            complement(745..1287)
                     /locus_tag="IV250_002026"
     CDS             complement(745..1287)
                     /locus_tag="IV250_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAP8769286.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            1422..1811
                     /locus_tag="IV250_002027"
     CDS             1422..1811
                     /locus_tag="IV250_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769287.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1815..2579
                     /locus_tag="IV250_002028"
     CDS             1815..2579
                     /locus_tag="IV250_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP8769288.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            2708..3655
                     /locus_tag="IV250_002029"
     CDS             2708..3655
                     /locus_tag="IV250_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8769289.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            3785..4525
                     /locus_tag="IV250_002030"
     CDS             3785..4525
                     /locus_tag="IV250_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769290.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(4816..5484)
                     /locus_tag="IV250_002031"
     CDS             complement(4816..5484)
                     /locus_tag="IV250_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8769291.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            5618..5905
                     /locus_tag="IV250_002032"
     CDS             5618..5905
                     /locus_tag="IV250_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769292.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            6321..7859
                     /locus_tag="IV250_002033"
     CDS             6321..7859
                     /locus_tag="IV250_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAP8769293.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            7856..8821
                     /locus_tag="IV250_002034"
     CDS             7856..8821
                     /locus_tag="IV250_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8769294.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            8808..10130
                     /locus_tag="IV250_002035"
     CDS             8808..10130
                     /locus_tag="IV250_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP8769295.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      10193..10297
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            10371..11114
                     /locus_tag="IV250_002036"
     CDS             10371..11114
                     /locus_tag="IV250_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8769296.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            11129..12121
                     /locus_tag="IV250_002037"
     CDS             11129..12121
                     /locus_tag="IV250_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8769297.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            12133..12870
                     /locus_tag="IV250_002038"
     CDS             12133..12870
                     /locus_tag="IV250_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8769298.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            12860..13582
                     /locus_tag="IV250_002039"
     CDS             12860..13582
                     /locus_tag="IV250_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP8769299.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(13666..14289)
                     /locus_tag="IV250_002040"
     CDS             complement(13666..14289)
                     /locus_tag="IV250_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8769300.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQP
                     VVQETVQTEKQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYI
                     GRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            14684..15375
                     /locus_tag="IV250_002041"
                     /pseudo
     CDS             14684..15375
                     /locus_tag="IV250_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
     gene            15583..15873
                     /locus_tag="IV250_002042"
     CDS             15583..15873
                     /locus_tag="IV250_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769301.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
BASE COUNT         5682 a         2759 c         3381 g         4795 t
ORIGIN      
        1 tctctgccac ataaatacac ctagactgac gatgaagagc atatccaaca gatttcctgc
       61 gacttggatc gtcgaagggt tggtcagtgg cattaaagca aggacaaaaa aggaaaacaa
      121 gctgtaaata aaacaggtga caggaactaa tcgaaagaag agcggacatt tcaattggct
      181 aaaaatgagt gaaaaaataa aataagtaaa aatggataaa atcacgacta aaccattcat
      241 gataaagaag aaaagcgtat tgtgattaaa cgaataaaaa agaattgcta aaaaagaaaa
      301 cgttttaaat gcaatcaata cgcttgaagc ttcgtttaac gtaccaggat gtcgatgaaa
      361 aaaataggca ccgaaataaa cagaaaacag taagccgcaa agagaagaca aaatcaaaaa
      421 aaacggattt tcttcggctt ctgtgatatc agaatgatcc aaaagcaacc agcctaatgc
      481 actagaaggc gcatgtgagg tcttaaggta tgaataacat agaaaaaata agatcgtcaa
      541 agttgcaaaa aaagagccta aatgtaaaaa attagtggtt ctacgttgaa tttccataat
      601 atctccaaat taaattgata agtatataaa gtataataac aaattgatga gtatatgtcg
      661 atgtttttta atcaaaataa taaataaaaa ctgtctaaca tttaaaattt aatttagata
      721 ttagacagtt tttaagtttg tgagttattt tccttgtcgt tttctcactt ttttagtcca
      781 gaaaccacct gaaatatatt ttggctcata agaaatgata aaagcacgct cttcgatttc
      841 tttgatcagg cgatacagtt cacgttcgct ttttctagga gaaagtattt cgagaactag
      901 ccgctcacct tccagcccca ttgcagaagt ctgtgtcaca ccataaccgt gttctctcag
      961 tatacccggc aaacttttat cttctgcgct atttgctggc aaaatcgttg tgaccatgat
     1021 gtatcctaat gccaatttgt cttcaatcaa aattccgaca ctgataccta ctgcgtaacc
     1081 taacgcatag acaaataaat tcagtggatt atctaaacga tctaaaacaa gggataagcc
     1141 gacgatataa atcgtgatct cgaccatact gacaagtgga gcaatcagtc gatagccttt
     1201 cattgtcaac atgaaacgta acgtgttcag agtgatataa gcaaaattaa tgaggaagat
     1261 catccccaac atttttggat caatcatttg taaaactcct ttcatttata taacaacaat
     1321 atcaaaaaaa atcgtaagtg aagaaaaatt gaaactttat acatacaaaa aaatgataaa
     1381 atgattataa tagaattata aagataggtt aggtgattaa aatgaaaacg gtcaatcgtg
     1441 tgttttgtgc cagccacgtg ctaagtctcc ttctcagtat atacttaggt gtctattcga
     1501 ttcaacagcc aattgaggta gagaacattg tttttcagat gtcgctgaca gatggtttaa
     1561 ttttaagcgt ttttttcttt ttgatactgt ttattggtaa tattttcggt agcgttgcca
     1621 gttttttgaa tttttctatt tatccactcc tttcgctagc attaggagtg gtgggacttt
     1681 gttctctgtg cttatttccc gagccattca gtcctgcatt tatattattt ggtacactga
     1741 acctgttcca gacaactgtt ggagcatggt tgctatggcg atcaggaaat ttaatgaaaa
     1801 taggtgagta acaattgaat ccaattatat tgaaacgttc tgtccatcat attccagtgt
     1861 tagaagttgt tctgaaagaa ttaagagagg aacgtattcc cgttgtcatc tattatcacg
     1921 gatggcagac aagtaaggag ttagttttga cgcaaggaag aaaaattgcg cagcagggca
     1981 ttcgtgtgat cctgccagat gcgatgaatc atggggagcg caaacagccg gtttcttcta
     2041 ttccatccta tacattttgg gatagtatct acggaaatct gtttgaattt gacacactta
     2101 tcaagcattt agaaaaaaga ggtcttctac atgatcagct tgcagttggc ggggtttcga
     2161 tggggggaat gacaacatgc gccttgttgg ctaaacatcc tcatatagta gcgggcgtgt
     2221 gtttgatggg ttcccctgca cctttggaat atggaaaaga aatcgtaagg cgcgcatcgg
     2281 aatataatta tgtcgtacca aaagactatt ttgatttgat ttcatgggtt aagagctatg
     2341 atttgtcatt gcagccggaa aagttagctg gacgtcctct gttcttttgg catggacaag
     2401 ctgacgaaaa aatcccttat atacaaacgg aacgatttgt agaaaaaaat cggcataagt
     2461 cttacggaaa agaaattgtt tttcgttcct caaaaacaca gcgacatatg gtacaaatac
     2521 cactaatgga agaagcttcc acttttttag caaaaaattt gataaaaaag tataaataat
     2581 tgaaacggaa aatcactcat gacgatagag gattttccgt tttttatttt gggctataat
     2641 aaactctgtt aggaaaattt ggtataattg atagtaagaa tgacaacatg ttagaaagga
     2701 agagagcatg cccactttag cagatgttgc aaaacgtgcc aatgtttcaa aaatgacggt
     2761 ctctcgagtc atcaatcatc cggaacaggt gactgacgaa ttgaaagagt tagttttctc
     2821 agcaatggct gaactggatt accgtccaaa tattgctgca aaagcacttg tcacgaatcg
     2881 gtcgcagatc atcaaattat ttatactgga agaaatcgac acaactgagc cctattacat
     2941 gaatttgctg atgggaatcg ctaaacggat cggtaaagcg cattattctc tccaattagt
     3001 tacaaatgat gccttcgacg tcggttcttg tgatggatac atcatcacag gtgtcagaaa
     3061 aaaagacttc gattggatcg accgtttgga aaaaccagtt gtcttgtttg gggaaaattc
     3121 tgaaggatat gattatgtag acagcgataa tgaatatggt acagcaaaag caacgcaata
     3181 tgccttagaa agagattatc aaacgattat ttatatcggt atcgaagtgg acgagccttt
     3241 tgaaatttca agggaaactg gttatttatc tgtgatgaag gaagttgatc ttcctagtaa
     3301 aatcttgcgg tttccaaatc attcgacaga agtagaaaga tatatgacag aaaactgggc
     3361 agattttaca gataaaactt gttttgtatg tagttcagat cggctagcac taggagcagt
     3421 aagagggatt ataaatgctg gaggacactt gcccgaagaa tttggtgtga ttggatttga
     3481 tggggtattt ttagaccaaa tcagttcacc taaattgaca acgatcaagc aagatattat
     3541 acgtatgggt gaagtatgtg gagaaatgtt gttaaaaaaa atagaagaaa aaggggcgag
     3601 tcaaggttat cgtcgttttt ttcctgaact agttatcaga gagacaacga attaagtctt
     3661 attactatat atatatttat gaaattcatc gctattttct taaaataatc agcgcattgc
     3721 tactgaaaac aatgaatctg tgctattatt gagaaaagtt ccatttttga gaaggatggt
     3781 ttgaatgaaa gaaatcagac gaaaacattt ccgttttcct gcgtacgatg atgagttagg
     3841 cgtaaaatta accaacgaca agaagcgagt attatttgac ggacaagaag atcttttgac
     3901 tgaacagaat cagtccccgt ttgaacagat gaaagaattt catttcacga acacgaacac
     3961 cgagaaaatc cgtcgcaagc gtcgtgcaac aaatggattg aagtcagctc cgcgtaagga
     4021 aaatttggaa aagcataaag gaaaattacc ggattatacc ggccattacc aatcagaaat
     4081 cacatcgaca ggacgaaaaa aactttttgg tgattcctct cgttctgtca gacaaagtga
     4141 aacaaggaca gagtccaagc atactacacg aaacgcatct gttgacacga aggtgcggga
     4201 gcgttcttat tttgttccta aatatgtacc cgcatcgatt atcccagacc ctagagaaag
     4261 caaaatttct gaggatcatt taatcgaatc gatgaaaaaa acggatgact cttatctgtt
     4321 gttcgataca gaaccgacgc cgtatcaaga aaaaagaaat ggtgagccgt ctgtacgaac
     4381 attcaatcga acaaaaacgt tagaggaaac aaagcagtca gaagaaaaaa aatcgaaaaa
     4441 aagccatggt atcttagaac gctcattaca aggattgatc gatgatcaag gaaactcttt
     4501 gggagaaaat agctatttta aataagtgat atacaaattt atattcggct acagttggaa
     4561 aggaaacgct gcggcgtttc cttattttat actctatccg aaagtttata tcgtatcctc
     4621 aaattttttt gagtttaagc gaattttttc tcccaataag taagaaaata atggcaaaat
     4681 aaaaaaacaa actattttcg cagatcgttt gcaaaaatag tttatttttt tataaagaag
     4741 attttactga ataatatcat tttagtttac ataatgatat ttctgtaaat taaataaagt
     4801 agtgaaaaat aagaatcagt gctctaactg ttcaagagga tcaagtggtt caaatgtttc
     4861 tgtagtctgt tttgcttcca attggggcaa aacagatcca atttccagat acttcacttt
     4921 attcaatgta taagatagaa tagaacagct aagtaatgta tacagaaaat gtgtgagatc
     4981 caaataaagc aaggagacaa ctaaaatacc aaaagtaatc aagaaaagac ttttgccaat
     5041 cttaatatga aaatattttt caatggcttg ataaatgata tctgttccgc ctgtgcttcc
     5101 gccgaatcga aagacgagac cgcaaccaat gccagaaaga atacctgtca tcaaactatt
     5161 gatcagtaga ctatctacag caaaatgtat atgaaggctc tcccataaat gtaaaaagaa
     5221 aactagcgca aatgttccga cgatcgtccg gagaaaagcg tgttttccaa aaagaaacaa
     5281 tgagataagc aataaaggta aattaaaaag cagtgaagtg acagaaggag cgaatccaaa
     5341 aagattatgc aaaagcaaag caataccagt aaccccacca tctgccagct tagatggaat
     5401 attgattcct gtcacagcta atccgtagat tcctgcgcct aaaatgattc cgcacaattc
     5461 tgtaaataaa tttaattttt tcatttctag cctcttttct tttgaaaatt ttcagcaact
     5521 tctttattat actgacttat actcttactt caagaggaaa atgaaaaaaa attatttttg
     5581 gctgtttctt tttatttatc aaataaggag tggaaaaatg cgagcagcag acttattgac
     5641 aattcttaca aaatcaccca aaaaaacaga gtatcaagga gtttttttgg ctcaagaaca
     5701 aaaactggtg cctgtgacaa tggtccagat aaataaagca aatcaattta tctttttatt
     5761 tgaacacggc aaagcaccac ttgctatgaa agacatcttt ctttttttga tgaagaaccg
     5821 tgagaaagct gtttattatc agtcaaaaga agaactcatt cctctttatg gagtgaaaga
     5881 gatagaaaat aaagtggtga tataagtaaa aataagaaaa caagacgttt atgatgtcaa
     5941 aacaaccatt ttttacattt ttggcaaaaa agtgtcatga tacagtataa tattcgttga
     6001 acaggccaat aaaggtgttc tcccccacga gatttctact ttattggctg tttcttttta
     6061 ttaaaataaa agttgtgtgt gtaaaaaaga tatagctgat tccaagtgac aatttcgtat
     6121 agatttttat ggtattcgta gtgaaaaaac gagaaccaaa aagttgatcc gactttgttg
     6181 cttggattct ttttttgtca gcatatttag aggtattttt ttcttttttt tgttcgtgct
     6241 gtaaggttaa ggcaagacaa gttagttggg aaaaaggaaa gaaatgttta tacttaaaga
     6301 ataacagaaa ggaaggtcgc atgaataatt ttatacaaac attaagtgaa agaaaaggcg
     6361 agttgctgga agctgcattt caacatctgt ccatttctct catttctttg ctgattgcag
     6421 cactgattgc tattccttta gcgatttggg cagccaatca taaaaaaatg gcagaagtct
     6481 tattgcagat cacgggtgtg ctccaaacga ttccttcact tgccttatta ggtttactta
     6541 tcccatttgt agggatcgga actgttccag cattgattgc acttgttatt tacgctttgc
     6601 ttccgatttt ccaaaacacg tatattggtc tagctgaaat tgacccgtct attgaagaag
     6661 cagcggtagc atttggtatg tccagaatga gacgcttgct gaaagtagag cttcccatcg
     6721 cattacctgt cattatctca ggtatccgaa ctgccttagt tttaatcatc ggtacggcta
     6781 ctttggcagc attgatcgga gcaggtggat tagggacatt tattttgtta ggtattgacc
     6841 gaaatacacc tgttttgaca ttgattggtg cgatcagttc tgccttgctt gcaattatat
     6901 tcagcggact gattcgctgg ttgcagcata agaaacctcg atattccatc attactttag
     6961 cagcagtggt gtgtttattt ggcggcgttt ctttatatca gtctgccttg tttaaaaatg
     7021 aaaccatcac gattgctgga aaattaggag cggaacctga aatcctgatc caaatgtata
     7081 aagaattgat tgaagacaac acaaaaacag aagttgaatt aaaaccgaat tttggcaaga
     7141 cgagtttttt gttcaatgca ctggatcaag gtcaaatcga tatctatcct gagttcacag
     7201 gtactgtcct agaaagcttg gtgaaggtac ctgaagcaat gaaaagtgct gatttgacaa
     7261 aagaaaaaac atatgacgaa gcagaagaat tattgaataa gcaattcgat atgacattat
     7321 taaaaccaat ggcttaccaa aatacgtatg ccttggcagt caagagatct tttgcgaacg
     7381 aacatcaact taaaaagatt tccgatttgg cagctatctc tgatcagatc aaagcgggat
     7441 tcacactgga atttattgat cgttcagatg gatataaagg aatccaagaa gcatatggct
     7501 tgaatttttc aagtgtacaa agcatggagc caagtttgcg ttaccaagca atcaataatg
     7561 gagatgtcaa tattgtggat gcctattcca cagatagcca gctcaaggag tacgatctag
     7621 tgactctaga agatgatcga gggttattcc cagcttatca aggcgcaccg ctgatgaaaa
     7681 aagattttgc taaagaacat cctgaaatcg ttaaagcact gaataaatta agcggaaaaa
     7741 taacagagga tcaaatgatt gatatgaatt accaagtcaa tgtcgaaaag aaaaagccga
     7801 cagaagtagc acgaatcttc ctgaaaaatg aaggattagt aaaggaggaa gatcagtgac
     7861 taaattaatc gagttcaaac atgtgcaaaa aaagtatgat ggaaagtatg tcatcgatga
     7921 cctaaatctg gcaatcaaaa aaggagaaat ctttgttttg gttggtcctt ccggtagtgg
     7981 aaaaacaact actttgaaaa tgatcaatgg attatctaaa ccatcagcag gagacatcta
     8041 cttcaaagga aaatcgttaa atgaatataa tctgcagaaa atgcggtgga atatgggata
     8101 tgtgctgcag caaatcgctt tgttcccgac catgacagtc aagcaaaata ttgaagtcat
     8161 ccctgaaatg ttaggttggg aaaagcagaa aagagcagac cgtgtagatg aacttcttca
     8221 aaaagttggc ttatctcctg atatttatcg agatcgtatg cctagagagt tgtctggagg
     8281 agaacagcaa cggatcggga tcatccgagc tattgctgca agcccagatg tgattttgat
     8341 ggacgagcca tttagcgcat tagatccgat ttcaagaaac tcattacaag aattggtttt
     8401 gtccttacac gaagaattgg gcacgaccat cgttttcgtt acgcacaata tggaagaagc
     8461 aatcaagcta ggtgatcgta ttgcatttat gaaggatgga gaaattatcc aatgtgacac
     8521 accagagcag ttattgatga atcctaaaaa cgactacgtt cggcatttct ttgatgaacc
     8581 gaaacagacg aaagagtggc gagtagaaga tttagtcgtc aatggatatt tcttaaatga
     8641 aataccagaa aatgctcgtc aacaagtttg ctttgatacg cctatgaaag aagtatattc
     8701 gcttctttcg gtgtatccaa gcattgtgat agtagaaaag caaagaagta ttgggtcgct
     8761 gacaagtaaa gaaatctttg cttttttaag tcgagaggag gaaggaaatg aaaacatatg
     8821 atgcaatcat catcggttca ggtgtgagcg gcttaagtgc tgcttatgga ttgaaagaag
     8881 caggtaaaac agtactagtg gtcgaagaag atctatgggg cgggacttgt cctaaccgtg
     8941 gatgtgatcc taaaaaagtc ttgttaagtg cagtagaggc acgtaatcgg gtaaaacaat
     9001 tgagtggaaa aggttttaac gagatcccaa ctgccaactg ggaagaactt caaaaattca
     9061 aacgaacatt tacggatcca gtcccagaaa gccgaaaaaa acaattagca gaagctgaaa
     9121 tcgatcatct ttcaggaacc gctcgttttt tggatgattc atctattgaa gtcaacgaag
     9181 aagtcttcca tgcggattac ttagttttgg caaccgggca aagacctacg atcttaccag
     9241 tggagggaaa ggaatacttg aaaactagtg cagacttttt atcattgcct gtactgccta
     9301 aagaaatcat ttttatcggt ggtggttata ttgcttttga gctagctacg attgccaatg
     9361 ctgcagggag caaagtaacc attgtccatc ataatcaacg gccattaaaa gaatttgagg
     9421 cgtctttggt agaggaagcc gtccatcaaa tggaagcatc tggcattcag tttgcttttg
     9481 gtgttgaaac ccaaaagatc atttcagaag gtactcgcta tcgtttagtc ggaaaagaga
     9541 cggaattagt agcagatatg attttttgtg cgactgggag acagccgaat acagaatctt
     9601 tagcacttga gcaagcgaat atcgtctttg ataaacacgg aattgcagtc aatgattatc
     9661 tgcaaacgag caatccaaaa atttttgcgt gtggagatat tgtttcaagg aaaaccccaa
     9721 aattgacacc agtcgccact tttgaaggaa attatgtagc taagcggata acggatgcaa
     9781 caagcgaacc tatcaaatat ccgattatac ctacgatcgt ctacgccagt cctaaacttg
     9841 ctgaagtagg agttacaaaa agtcacgcct cttcgtcaga tcaagtggta gagatggatc
     9901 ttaccagttg gttcacctat catcgtgtca atgaaccagt tgctaaggct gaattgacct
     9961 ttgaccagca aaattatttg attggtgctg cagtgattag cgagcaagca gacgaattga
    10021 tcgatgattt gacactcgtc atcaatcaaa aattgactaa aaaagagttg gatagctata
    10081 tcatggggta tccgacattg gcaagtgatc tctcctattt attgaaataa tcagcatgat
    10141 aactaaagcg cttgttgctt tttccttctt gtgctacact ttaaaaaatg aatacctgtt
    10201 tctggggatg tcgtagacga catgagagta agttgtgaac ttagacccat tgaacctgtc
    10261 agttaatact ggtgaaggga gaaacatagc agacgcttga agcgggttac tatgtcgtgc
    10321 ccatttcaag cgttttttgt tattcatatt agaagaaagg aggtaacaaa atgaagaaaa
    10381 atctttatcc agcagtcagt atggcattca ttttaattat ttggcagatt gttgtcatag
    10441 tttggaaaat acctgccttt attttacccg gacctgctgc tgtattttct gcattagcgg
    10501 cagatgcaca agtattactt tcccattctg tcacgacatt aaatgaagca gtgcttggtt
    10561 tggtaatagc tagcgttctt tcttttatta ctgccttggc aatggattat tggaagtttt
    10621 tagaaatgtc tttttatccg ttactagtga tttcacaaat gttgccaatc atggtgttag
    10681 ggccattact taccctatgg tttggtttcg gtatgcttcc aaaagtgatc cttgttgtct
    10741 tgataagcta ttttccgatc gtagtttctt tttcggatac attgaggaaa gtatcaaaag
    10801 agcaaatcat ctttttgaaa acgatggggg cagatacagg gaagatttat cgaatatata
    10861 aaataccagc gggtttatcc ggcttctttt ccggtttaaa agtggctgct acttattgtg
    10921 tcagcggagc agtcgttgga gaatggctaa gcgctcaagc aggcttaggg tattacatga
    10981 tacgcgtaaa aaacagctac cagatcgata aagtttttgc tgcgatcatt tgtgtcattc
    11041 tattgagttt attgctgaat ggcgcttgtt cattattgaa aaaagggtat tatcgtatgc
    11101 tttatatttt ttaagagaag gggatcaaat gaaaaaatta tggcttttat taccgctact
    11161 gttgctttta tccgcttgtg gcactgcaaa agaaacaagt agcaaacaag aaataaagga
    11221 tttgaaaaaa gtgacacttg ttttggatta tgtaccgaat actaatcata ctggcatcta
    11281 cctagcaaaa gaaaaaggct attataaaga ggctggttta aatgtccaaa tcatcgaacc
    11341 aggagataat tcgacaagta ttggtctagt aggtgcggac aaagcacaat ttggtgtaag
    11401 ttatcaagaa gatgtcactt atgctcacgc agatggacag aatataccag tcaaagcaat
    11461 tgctacagtg atcaaacata atacttctgg attcgccacc ctatcagaca gtaacatcca
    11521 ttcaccaaaa gattttgaag gaaaaacata tgcaggttgg caaagtccaa gtgaagaagc
    11581 tgtcttaaaa gctgtgatgg aaaaagacgg cggggacttt tcaaaactga caatggtagg
    11641 aagcaatgga gaagggccag aaagcttagg aaaatcctca gatatccaat ggtactttga
    11701 aggttgggat atgatcaaag caaaagaagc gggtattgaa ctaaactata tcccattgaa
    11761 agaattagat gaacggttag attactatac tccggtaatt attacgaatg atcaactgat
    11821 caaaagtgat ccagaattgg tacagtcttt tatggatgcc acgaaaaaag gctatcagga
    11881 agctattaag gatccaaatg acagtgctaa actgttgcaa aaatatgcaa aagaaaatga
    11941 tcggacgttt ttagaagaat cacaagcatt cttgtcaaaa aattatacgg atgatcctga
    12001 gaattgggga ttgatggaag aaaaagtttg gcgcaattat acagctttta tgcaggaaaa
    12061 cggattgatc aagcaagatg taccttcgca agaactattc actaatcagt ttataaaata
    12121 ggagtgatag atatgtcaat ccaaatcagc cacttgaatg tagtcattga aaacctgaaa
    12181 atattagaag acatccattt cactatccct aaagatacct ttacatcgat tgtcgcgcca
    12241 agcggagccg gtaagtcaac attgctgaaa actttaacag gagtccagcc tatgacttct
    12301 ggaacgatca aagtagacga tcaaaaagta actggtctcc atacagcatt tagttatatg
    12361 ccgcaagaag atatgttact tccttggctg agcgtctatc aaaacgttac gttatatcaa
    12421 aaaatcaatc atctaacaat caatgaagaa caagtaaggg attatctgga tatctttggg
    12481 ttaaacggct atgaacattt tttgccggaa caattatctg gcgggatgaa gcagcgaacg
    12541 gccttgttac gaacaatcat gaatccttct tcttacttgc ttcttgatga accatttgga
    12601 gcgttagatg ctatgaccag agggcagatg caagactgga tgttgaagct gcctaaaaaa
    12661 gcaaagcgga ctactttact tgtgacgcat gatatcgaag aagcaattta tttatcagac
    12721 aggattcttg tgctttccgc ccgtccggca catgtgattg ctgagattca agtgcctgaa
    12781 aaacgacggt cacgtgaatg gctactgcag caatcagagt tgaagcaaac gatttatcag
    12841 ttattggcag gtgaattaga tgttaagtga tgctcattgt catttagatg ggagtcagag
    12901 tttagcactc cttcagcagg aacacacgat tttgacgatc atcaattgtg attcgccaga
    12961 agaatggaag gaaaacagac agcttgcagc aagtaagact caagccttaa gttacggtat
    13021 ccatccttgg aaagcagata gctatacgtt tgaacaagtt gagccttttt tgaaaaaagc
    13081 aaggatcatc ggtgaaatag ggttagataa tatttggacg aatgtaccga tgactacaca
    13141 aaaaaaagta tttgagcgac agcttgcttt tgcagccata aatgagaaac ctgttgtact
    13201 gcacacaaaa ggatgcgaaa aagagatctt aaattatatc caagcttacc ctaatcacta
    13261 tttgatccat tggtattctt ctttggacta tcaacaagac tatattgatt taggctgtta
    13321 tttcagcatt ggtcttgatt tgaaaaagaa tccagcagtc tggcaactag ctaaagcagt
    13381 cccaatcgac cacttactta ttgaaacgga tggactacat gcagcagagt gggctttagg
    13441 ggggattttt tcagaaaaag attatgtctc actgcttgaa gaacagttaa agacaatcgc
    13501 tgagatcaaa gagctatcag ttgaagaagt taaaaaaaat acttatcaaa acttggaacg
    13561 tttcattaga ttaggtaaat aaaaaagaga tgtgacacaa ctagtgtcgc acctcttttt
    13621 ggctttcttt gtataaaagt aaactgtttg attaataaat gaatcttagt accaaccgtt
    13681 tgcttcccag aatgctttcg ccgcttccca agaaccataa cgtgaagtaa catattgttc
    13741 tgctactctt tcttggtttg cagcagagta gtcaccattt aagtaagaag aatctaattg
    13801 gtaacgtccg atgtaacgtc cgttcgttgc tgtgtaagaa ccgcttgatt ctttttgtgc
    13861 gatccattct tttgcagagc ttgtgcttgc tggagtagca gcagtttctg ttacagcagg
    13921 agcaggttgt gtttcagcaa caggcgctgc ttgtttttcc gtttgaaccg tttcttgtac
    13981 aacaggttgt tcagcttgga ctggtgcttc ttgaacggct gcttgaggtt cagcagctgc
    14041 ttgtgcagag ccttctgttg ggattgttaa ttgttgtcct gagtaaatca ggttaatatc
    14101 agaaattgaa tttgattctg caatggcatt gatcaaggag ttatcaccaa cgtatttttg
    14161 agaaatcgtt gatagagtat cacctgattg aacggtataa gcttcgtccg catgagcagt
    14221 cgttcccatg aagaatgcag cgccggcagc caaagttgtt ccaaaaagta aagttttaag
    14281 tgatgtcatg aaaaatctcc tttattcgtt aaaaagtttt atttgttgtg ttcaacgagg
    14341 aatactttaa cacgtgaaaa tatttcatag gtaaaagaag catgacaatt tatgacaaat
    14401 ccgcgttttt tgtaataaat acagtaatct atgaaatgaa aagggttacg tttaataaaa
    14461 cttgcttttt tgtttttgtt caccataaat atttatttaa gaaagtatta acctgcattt
    14521 ttctctataa aaaagatttt ttaaatagat aaaaaaggag gattaaatga gtatttgata
    14581 gtatttatat taaaattaca tatgtaattt aaaaacgctt gacattgtgt ttgagatcac
    14641 ttacttttag catgtaaaag tattggatta aggagctatg caaatgaaaa agaaatttat
    14701 tactggtggg attgtcgttg catttctatg tctaattggc tacacaggca cgcggtacgc
    14761 aaatgaaatt attttgtggg gtggagagac agatattgat acaatcaatg agaatctgac
    14821 acagcttgat catacattga cagataaaga gcaagcgatt acagatctta ccgctcgact
    14881 agcacaaaaa gaacagacat tgcaagattc aaaggcagat gtccaagaat accaaacaaa
    14941 agtaaatgaa ttagaaaacc aaaaaaatca atcggcagca gaaaaacaaa acttagaaag
    15001 tcaattagtc agcaaggatg cacaactaca aagcaaacaa aatgaaatca atgtcaaact
    15061 agatgagatc aaccaaaaaa atgatgaaat caatcatctg aatgcagatt ggacagcaag
    15121 actacaagaa gcacaagcaa aactttcaga attacaaaat actgtaaatg gtttgaatca
    15181 acaaatcgca acactgacac aagaaaaaac aaatgtagaa atcagttaac agatacacaa
    15241 aagaaattag acgaagcaac tgctgaaaac tcaagcttga aacaatatat tcaaaagtta
    15301 gaaaaagcaa aagatgaagt ggaagataca gctgataaat ctcagcaaat tgttgaagaa
    15361 catttgcgta aataatcttt cttaacaagt aaacctaaat tagtaaagaa gcaacagtaa
    15421 tctatactgc tgcctctttt ttttagtaca tgtccttcat cttcgcagga agtgccaata
    15481 tgattttaag aaaggtaata gacaaaaagt caaaaagata aaacagagtt tcttctttac
    15541 aaagggaatg tatgttcgta taatggtatt gaggtgaagg ttatggagaa aaaatatgga
    15601 acagtagata agatccgtac tttgcaagtt agtgccagac cgttgatccg tttcagttta
    15661 aatggggtca attgtttgat tgcttcccat agtttgaact tcctagctga agtagacgaa
    15721 gggatgaagt tagtcgttac aggttattat aataatagaa agcaatttgt tgtgagatcc
    15781 tatcatgtgt tagggaaacc caaaattgtg gtagaatatg agaaaagtct atacccaaga
    15841 aaaaaggtcc gacaagatga aagaataaac tagaaaacaa gcggctgaga caaaaatgtc
    15901 ctagccgctt gttttggaaa gatggatgca gacgatggga tttgaaccca cacgagcata
    15961 cgctcacaag aacctcaatc tatatataac tacatgtatt taagtatttg tgatgaatca
    16021 ctaaactttc caaactcatt tgagggatat tgcttaaaga ttgaacagat caattcgaag
    16081 ccctattagt attcgctcaa tggggggatc agaggccata aaaagagact agtcattact
    16141 agtctcaggt agtggaattg taggatttta aacgttcctg gagagtaccg gtatgtaact
    16201 cgaagaggtg attatcatag tcgtaaaagt aaattgaaga agcctcgcct ttcacacgag
    16261 aacgtcctgg ttcgacagtt aaacctaaca tttggatttt tgagacaaac gaatctatat
    16321 cttgttcgtc aattttaaaa gcaatatgat tgtatgtctt tggcagttta ttcgacgaat
    16381 tctgcattac tgcaatccat aggtccttaa ctaagaaaaa tttttctgga tataatgaaa
    16441 atttttttgt tgactatcgt agatttcttt tgcatcaaat aaggtattta acaattaagt
    16501 atttaaaaga taacttttaa gttatcttta agtaatgctt aaagctatgt tatactctat
    16561 taggaaatat aagaaggagt ataaaaatga acaaacataa tagaaaaata acgaata
//
LOCUS       DACDIE010000052        16569 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.052, whole genome shotgun sequence.
ACCESSION   DACDIE010000052 DACDIE010000000
VERSION     DACDIE010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16569
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.052"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            21..344
                     /locus_tag="IV250_002043"
     CDS             21..344
                     /locus_tag="IV250_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010714055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769302.1"
                     /translation="MSLTVRVLVQKVGDLSDYYNCKREVIVDNRYDQSKDDKRIIKIG
                     DIVFPEKGIAYEVVSINSFRYEFVDLVVEYSVRLIVPWSKEEINEALKDERKSTFKVL
                     EGEKK"
     gene            341..820
                     /locus_tag="IV250_002044"
     CDS             341..820
                     /locus_tag="IV250_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769303.1"
                     /translation="MKTDLTRQAEKCLWHETNRMGVFGCFEVTIGWDGRERVDYITYS
                     VDNTVRCYEIKVTLSDLKSKAKQTFIGDYNYLVVTSDLWEKMLQDDTLMRKYYKQGVY
                     VFNEKYPSMISVKKARKQKVSLGTRSTVLESMVRSMNREVKKLYKVKPFWEIVEIPE"
     gene            959..1498
                     /locus_tag="IV250_002045"
     CDS             959..1498
                     /locus_tag="IV250_002045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769304.1"
                     /translation="MNKRRILWLIAGMYLCIAVSGFTICRIINQLKNAIVYLGEIGYL
                     EDLKAGLQIMSWILFVLTVALVILTCENFSTIKKQIISNHNQETVKSGLEVDPEEEVT
                     EQAIKEQEEIDKKEIMSIYLSSGESFFHEIDSLKLSSQEIIDRWQNMSLDGELIVEDS
                     EGWSAYKKDEIVAITILFV"
     gene            2149..2202
                     /locus_tag="IV250_002046"
                     /pseudo
     CDS             2149..2202
                     /locus_tag="IV250_002046"
                     /inference="COORDINATES: protein motif:HMM:NF019230.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2206..2805
                     /locus_tag="IV250_002047"
     CDS             2206..2805
                     /locus_tag="IV250_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAP8769305.1"
                     /translation="MREKNKNSSRYLVATVVFVCVLIFLFDFLQIGKSIIGNTLSAKE
                     MVALGGLYEGHLFPNIFISIVSHINLYHLISNLLFLVVLEEALNDQYEWLTLLIVLII
                     SGFLGSLVPFLLQSNIVSMGLSGGIFGWIALFAIAYIHFDSKEHLLLSVFVLLIIGIL
                     LVSTFESGEANVLSHVIGFVCGMILGFILKNVSLIKQKN"
     gene            2821..3297
                     /locus_tag="IV250_002048"
     CDS             2821..3297
                     /locus_tag="IV250_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769306.1"
                     /translation="MPKRKEKKIPVFLSVSEMLSLQILLRKKIETIDEEVRNKRAMHE
                     QREALRERIHYFDDIHTKIYEARTNHEDESKLGLAQQRILEWLNTNTDQEPLRVISNL
                     FEFGTKHWFNAVEKDADLQEAFDTLSKDSKSMRDLLITYLNEHEFEQTKTKKKNKE"
     gene            3314..4216
                     /locus_tag="IV250_002049"
     CDS             3314..4216
                     /locus_tag="IV250_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769307.1"
                     /translation="MLLTLLGYLCLILAFFGAIAIIIGQFQTNNPIMRNIGIGIVIVT
                     FIVFNATKIFGSNDKQFGISGVNNSYAPDSLGRVVIDGTFENVTSYKNVSLTVKEDGK
                     KASDDVLATIKPSQSQFEVFYTVKENSPVHKLTLVFNVDGEEYYKDVKINKTSTKKII
                     ESAKKEVATEESTTESSSTVEAELPDKIDFDPEKVKSFESVSGSMADRFNTEVFKAIK
                     TVKLDTEKQMINVTLFNSVESMDQDKKVTVARELGKRVIGLYRGTAKPDSDGADLPVH
                     VVYEDGKVFGDSTANEALTIKLAK"
     gene            4289..4480
                     /locus_tag="IV250_002050"
     CDS             4289..4480
                     /locus_tag="IV250_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769308.1"
                     /translation="MVVNKEKVRKYVTLKIASVKYLEKRAKEEERARGKKVTVSELLE
                     ELIENDQTIRRAFTNRSIR"
     gene            4645..5232
                     /locus_tag="IV250_002051"
     CDS             4645..5232
                     /locus_tag="IV250_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769309.1"
                     /translation="MENNNDIRHFSIGERVTTVSELSKDYRDKEGTIISLNKNRRGVW
                     QYVVDLGDGVTVECKDSQLIRNNGSHSIEKAILVYPKVSSKIMNDWLLGKFGEYNKKE
                     IAEVIGKSYPTVLGYLKKITSKEAKIEDVLTVRELQSLIEYRNKQITNPEYLRKEDGL
                     LKQLEQVNKIRKMQKVAPLSLDTFKKIYLKEIKKD"
     gene            5232..6131
                     /locus_tag="IV250_002052"
     CDS             5232..6131
                     /locus_tag="IV250_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HAP8769310.1"
                     /translation="MWTVTLPFQPVSAPRPSARRNAADETNDVAEKRISTYYDKKYLD
                     YLKKIKSFIEDQDLLDDEFYSIVNDPMGAIMEVDFYYQIPKSYKKVYNIMKTTAPDLD
                     NLVKSAMDGIFNISAIRDSHITGLIAFKYNVANEGKTVVRIKRYSEFEWDTSEGLNGD
                     IWEATFDFKPVATPRPKSKFRGNGIITYYPKEYNDYLENIKNTLKEKDLVNDQLKKVM
                     NAPMGVIAQIDYFYQVRKDKKKLDSLMRTSQPDIDNLVKGTLDGIFNKEIGIKDSRVV
                     GLIAFKYNTFEKSKTNVRLQEMG"
     gene            6131..7045
                     /locus_tag="IV250_002053"
     CDS             6131..7045
                     /locus_tag="IV250_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769311.1"
                     /translation="MNNNEKILHVLDSFEIIQEELKKYRDVLEQRYDFVNQQKSNHMD
                     FILNMNDLKKKLVERKEQEKLIKAYFELGEKEVKNAMELNEERRVLDQLLEQLLVMFQ
                     KGRIDEDLIEEGLRKYPSNSGIGIVLKAIDEEEIEAFIPPEDFESAMEYIKYYSQGIT
                     AFREFDPEDVIHDLNNLKEWCESYGVDDSGLDYLISIMEIEEEMPDKPDPTDILELIH
                     EARNPIAYISRGYTVLEYYKPYISAMNHLRRVLREKREYRSVLNATNRLEKAVSELDA
                     YYREHYLQAGGMPRNTKANISRYIKKAE"
     gene            7097..7312
                     /locus_tag="IV250_002054"
     CDS             7097..7312
                     /locus_tag="IV250_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769312.1"
                     /translation="MKRDVFELYVLERLEDGMMAQEIIDEIDDMISRFGQLELNDGNK
                     NSVVLKKFWLNNFSYKDFIKEKANTLY"
     gene            7502..7756
                     /locus_tag="IV250_002055"
     CDS             7502..7756
                     /locus_tag="IV250_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3034 family protein"
                     /protein_id="HAP8769313.1"
                     /translation="MKKEELIDIFQIVERANNMGIMFFDRISLKMDLSVAHQEFNLRL
                     KALLNSDDVNFAHDVVGIQNHIDRENKRMGDEFLPRYSSL"
     gene            7935..8978
                     /locus_tag="IV250_002056"
     CDS             7935..8978
                     /locus_tag="IV250_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyhydrolase"
                     /protein_id="HAP8769314.1"
                     /translation="MKKFLVGILFTMGLVLLSPSVSAESSISMYRMYNPNSGEHLYTR
                     SVGERDNLKRYGWHYEGVAWQAPTSGTPVYRLYNRYNGEHFYTVDAGERDKISHTGWT
                     YEGVAFYSYNGANRVPVTRLYNKYVNWHHYTLDQNEKNVISKQGWKIEGTGWYAMPNS
                     VPNPTPAPSTEKVLNVPVVWQGNTMLCEGASLLEGLHYKGVATNQSLTSFVNYMPKAN
                     DNNPYHGYSGEWRHNVNGTYQGMMADPVVKWAKNVGGNAVNITGSGTNGIKNEIRNGN
                     PVVTWITYNYATPEFKQMPWGRAVWNGHVVLVDGFRNGAYHVVDPVFGAKWINASTYE
                     RAFNVTGMAVAVR"
     gene            9151..9375
                     /locus_tag="IV250_002057"
     CDS             9151..9375
                     /locus_tag="IV250_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769315.1"
                     /translation="MNQRRRKRLEEISERLSELKDELEVIQEEEYKENIPKNLQGSER
                     YEKAEESVDQLEEAVDGLSDILELIEDIAS"
     gene            9378..9635
                     /locus_tag="IV250_002058"
     CDS             9378..9635
                     /locus_tag="IV250_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769316.1"
                     /translation="MINEDFRSEQKMIDISLIEKLLFSNKYTAKELATRWQISESAIK
                     AYRQNKGSVGWRDWKGISIGKAEDIMKREGFQADNDSKKVE"
     gene            9852..10727
                     /locus_tag="IV250_002059"
     CDS             9852..10727
                     /locus_tag="IV250_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769317.1"
                     /translation="MTTNKRWSELVQNTVKKVMPDGYHFNYIQCSQTTESIYFSILNE
                     NRLYYFRVSSHHRKKREDNVTTFLSGKYNGFKELSEAIGEYLKSNRFVRISDKHILIL
                     LLYLNNSFKLFWRDDTFYYIENGMDIYSGINGILKHYIKALLRYGVLYQEDLSNQIKI
                     SNSGKSLLAKYKKLLSPIYKGIDMEAVNSETLFNLVVDEKFANEFFKSKKIERLNNLF
                     PENAIKAYVDNNKSRLYNWFIPKRLRGKIKIGDQVKVINLNHTKIVTVTELYIAEKED
                     ITKMKPVIKILDNKE"
     gene            10737..11531
                     /locus_tag="IV250_002060"
     CDS             10737..11531
                     /locus_tag="IV250_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769318.1"
                     /translation="MVAKKNTLKEASEKYQDMKRRGLIPKDKKGIWREFVKEVSSDRS
                     GGDFVGRYTAKGYSRRLRITVEYGTNESATELKQRYLDTRREASKKYDDFKSRGLIPK
                     KKKGIWREFFKEEDSNRNKGDFIGRYTAKGYTKNLGIKVEYGTGESSWELRNRYLNTL
                     LEKYKAELLELFDVNDFIEMAEKYGHEEGFMINDIMVIDNFTDLLALAKKARDLRETQ
                     MYLFRNKNGRNRRTESSEIEDYYDAFTHDLGAFVDEISGVMNKYRV"
     gene            11560..12678
                     /locus_tag="IV250_002061"
     CDS             11560..12678
                     /locus_tag="IV250_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769319.1"
                     /translation="MGNNSIYTPEMQNKIKRLVGLGWMTRGKLDSFSTIKYLLMDIYR
                     QNKQTGIHFLGYDKPEDAPFFVPSSAYRASTSGSMYNLDLFEEGAKMIEYDFNEAYSR
                     IMRNYPLPSNTYLKAKYNTDQAILRAINRPKVRPYENLKTYMFFLVNFEGTAKEGTYA
                     NPKSQSMFADYGENPKTANEPIWLSEIELTLILDYYDIKEMEVLDSHTFLTKKGLLKE
                     YFERIEPLKTDPDTLKLYKNLRTKVFGAIGQKSLREGETSTFDKYSMYNRAYSSMVAG
                     VFRDRMVRYEQKYVHSAYGLLKIRTDGLYFRSEVPEFEELYKLGVVKKKVHTISKEEV
                     EKALEFKNKGFENKGSFGKSKDEVKGVVIDDDFEFDFD"
     gene            12711..13031
                     /locus_tag="IV250_002062"
     CDS             12711..13031
                     /locus_tag="IV250_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769320.1"
                     /translation="MKKKIVEGNVGVIILKDSTDYAKSMALKDEKVARMDKDLVEFLM
                     DDTERLKGDQFLSLMKDKGLNTYGLFDYKKLAIEWVKEGEIFFIEEWEGTENLVLHQY
                     LLKA"
     gene            13286..14179
                     /locus_tag="IV250_002063"
     CDS             13286..14179
                     /locus_tag="IV250_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769321.1"
                     /translation="MRTHYIYLEDKIVDEIPAKSREQARKLFIEKYKEINGDSEVPKV
                     VVTVRKRQTKKRSTFHVSPDIYNLIERHRSEDNISISQYIATVIDELISGKYDYAFDF
                     EDEKDYKSVAVFQSDFEKLKNYAGRRNVNFARLLEEFYSEELGVGNEYKRETYAKLSH
                     KLSQALKEVTEVKAELDAKLEEVKKYQTERKNNMKNQVEFDEQKEFEKARKRYNKIMN
                     ELSYEHLTVHEELSEGTEKYTIADMVNEVETLLASFFENGHVNDEWRKEEPKRVNAYI
                     ARLKRFIDKYKENSDKFSKDK"
     gene            14199..15827
                     /locus_tag="IV250_002064"
     CDS             14199..15827
                     /locus_tag="IV250_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769322.1"
                     /translation="MPEIEGIFSPKIGKKQLDFSSFPSVNRDYNLGDEVQILNIDFKE
                     LDFAVAVSNKPKALERVKKIYQENDQNIKNMIKKYRRDNSKVEDVLFNGTAKHDLNVY
                     MALGVALYCNSINQTDILVNIVRNSYQKILKISSETNPYYAMKYLGDNLLNDKKLMHN
                     TEELLSNLAAINFLFKTGSEDYFIDNVLKGAYGTAFYPLYNMKQHKAEQKDNKLEIKK
                     MKELLGIGFVKPNSKVTIASLATTNNDGKIVNGFDDSDIDILSKSYFSLYGDFADLMG
                     IDSYLVNNFEIKGSELNDIVYFVLISLYAIDQNTEYRQIDYLLVFYSLLYQFVLTRLF
                     KNAKEELILNSDYEIAKQIASIRKENKSEVIKLRKELSIASKENGRLESLVKSAKKEE
                     ELLKQEIKELKGIRKEFEQLRFENKVLKQLQEQEHRQSLNGKTEEPSLSEMIAYLKHK
                     NILLVGGHVNFQNKLTELLPNIKMFDVSENNGKILKNLNTCDAIFLYTDYLNHGLYYR
                     VMSEISNKDKIFYLDNHVNMNLTIKRIYDLLSLI"
     gene            15878..16246
                     /locus_tag="IV250_002065"
                     /pseudo
     CDS             15878..16246
                     /locus_tag="IV250_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            16346..16546
                     /locus_tag="IV250_002066"
     CDS             16346..16546
                     /locus_tag="IV250_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769323.1"
                     /translation="MLKRIAWDKDNKTVIELKEYKYNYFSRCIDRKLLFKKEGLEFSF
                     DEDEFEKLVMDWRKNLLDTVQF"
BASE COUNT         6367 a         2045 c         3379 g         4778 t
ORIGIN      
        1 aacattattt tagcaaatct attagtttaa cagttagagt tctagttcag aaagtcggag
       61 acttgagtga ttattataac tgcaaaagag aagttattgt ggataatcga tacgaccaat
      121 caaaagatga taagcgaata ataaaaatag gagatattgt ctttcctgag aaagggatag
      181 catatgaagt agtgtctatc aatagttttc gctatgaatt tgttgatttg gttgtcgaat
      241 attctgttcg attgatcgtt ccgtggagta aagaagaaat taatgaagca ttaaaagatg
      301 aacgaaaaag tacattcaaa gttttagaag gtgaaaaaaa gtgaaaacag accttactag
      361 acaagctgag aagtgtttat ggcatgaaac aaatcgcatg ggtgtatttg gttgttttga
      421 agtaactatc ggttgggatg gcagagaaag agtagattat ataacttatt cagtagataa
      481 cacagttcga tgttatgaga tcaaagttac tttatccgat ttgaaaagca aggcgaaaca
      541 gacatttatc ggcgattata actatttagt cgttactagt gatttgtggg agaaaatgct
      601 acaagacgac acactcatgc gtaaatatta taaacaaggc gtatacgtgt ttaatgaaaa
      661 atatcctagc atgataagtg ttaagaaagc aaggaaacaa aaagtttcgc taggtacaag
      721 atcaactgta ttagaaagca tggttagatc gatgaatcgt gaagttaaga aattatataa
      781 agtaaaacca ttttgggaga tagtagagat accggaatga tgagtaagga atattatatc
      841 tattcatata tagtattaac tgattatgag gaggatacag caaatgaaaa attatgatcc
      901 gaatatgcga tggggtacac atactcttaa agtgtctttt caacggagga taagctaaat
      961 gaataaaaga agaatactgt ggcttattgc aggaatgtat ctttgcatag cagtatctgg
     1021 gtttactatt tgtcggataa taaatcagtt aaaaaacgcg attgtttatc taggagaaat
     1081 tggttatcta gaagatttaa aggcaggact acaaataatg agttggattt tattcgttct
     1141 gacagttgcg ttagttatat taacttgtga aaatttttca acaataaaaa aacaaataat
     1201 atctaaccat aatcaagaga ctgttaaatc aggtctagaa gtagacccag aagaggaagt
     1261 aactgaacaa gcgatcaaag agcaggaaga aatagacaaa aaagaaataa tgagtattta
     1321 tttatctagt ggagagtcat tttttcatga aatagattcg ctgaaactta gtagtcaaga
     1381 aataattgat agatggcaaa atatgtcttt agatggggag ttaatcgttg aggatagtga
     1441 aggttggtca gcttataaga aggacgagat tgtagctatc acgattttgt ttgtatagta
     1501 atatatacca ctagataatc tgaatatgtt gatatgctag tgacgtacca aatgtgttca
     1561 atagagccgt ggtcacaggc agaaattgat aaaatagtaa aatttaaccg attatcaaaa
     1621 tttaacgttt taggaaatac cttttaaaaa tcacaataca caaacagaaa aataccaaaa
     1681 aattaacaat gaacgtaggt aagaacatga caataaatga taataacctc aaaaaatata
     1741 atgctcttgt ccaacgctat atcgaaaaca aaataacgat gcaagaggtc gctgatgaat
     1801 taggcaaatc aaaaagccta gtatcacacg actttaagca caatgttttt ccgaacccga
     1861 ttgagctgag gaaaaaattt caaaagagag tttatcaaga aatagggaag tggattgatg
     1921 aagggaatgc aattgaccct ttaattgaaa aatatccttt tttaaaaagc aaaaataaaa
     1981 ttcaaaggtt aattcgtcaa ggcgatattc aagctaaaga acctgttttg acaaccaatg
     2041 atttggctaa aataaatctt cataaacaaa ttattgaaga ctttgaaaaa aatctttcaa
     2101 caactgaaat tttaaaaaaa tacaatattc cgagaggaaa gctaacgtcg actcttttta
     2161 gagaatatgg agaatcggca gttactaaag caaaggaagt agcgaatgag agaaaaaaac
     2221 aaaaatagta gtcgatactt agtagcgact gtagtttttg tttgtgtatt gattttcctg
     2281 tttgattttt tacagattgg aaaaagtata attggaaata cattgagtgc aaaggaaatg
     2341 gttgctttag gtggtttgta tgaaggacat ttgttcccga atatattcat ttctatcgtt
     2401 tctcatatta acttgtacca tttaatttct aatttattgt tcttagtagt tcttgaagaa
     2461 gcactgaatg atcagtatga gtggttaaca ttattgatcg ttttaatcat atcaggattt
     2521 ctaggaagct tagttccttt tctgctacaa agcaacatcg taagtatggg gcttagtgga
     2581 ggtatttttg gatggattgc tttatttgcg attgcataca tccattttga tagcaaggaa
     2641 catttattac taagtgtttt tgtattattg ataatcggca ttttattggt atctactttt
     2701 gaatcaggcg aggcaaatgt cttgtcacat gtaatcggat ttgtctgtgg aatgatcttg
     2761 ggatttattc taaagaacgt ttcattgatc aaacaaaaga attaaaaggg agtgctaaaa
     2821 atgccaaaaa gaaaagaaaa gaaaattcca gtgtttttgt ctgtctcaga aatgttatca
     2881 cttcaaatac tgttaagaaa aaagattgaa acaatagacg aagaagttag aaataaaagg
     2941 gctatgcacg aacaacgtga agcgctaaga gaacgaatcc actattttga tgatatacac
     3001 actaaaatct atgaagcacg tacaaatcac gaagacgaaa gtaaattagg attagctcaa
     3061 caacggatac ttgaatggct taatacgaat actgatcaag aaccgttaag agtgattagt
     3121 aatctttttg aatttggaac gaagcattgg tttaatgctg tagagaagga tgctgactta
     3181 caagaagcgt ttgatacgct aagtaaggat agcaaatcca tgcgagattt attgattact
     3241 tatctaaatg agcatgagtt tgaacaaacg aaaaccaaaa agaaaaataa agaatagaaa
     3301 gttgtgatga atcatgctat taaccctgtt aggttattta tgtttaattc ttgctttttt
     3361 tggggcaatc gctataatta ttggtcaatt tcaaacaaat aatccgatta tgcgtaacat
     3421 aggcattgga attgtgattg taacctttat tgtgtttaat gccactaaga tttttggaag
     3481 taacgataaa caatttggta tatcgggagt taataatagt tatgctccag attctttggg
     3541 aagggtagta atagatggaa catttgaaaa tgtaacgagt tataaaaatg tttcgctgac
     3601 agtgaaggaa gatggcaaga aagcatcaga cgacgttctc gctactatta agccgtctca
     3661 atcacaattt gaagtgtttt atacagttaa agagaatagc cctgtacaca agctgacatt
     3721 ggtttttaac gttgatggag aagaatacta taaagatgta aaaataaata aaacatcaac
     3781 gaaaaagatt atcgaatcag cgaaaaaaga agtagctaca gaagaaagta caactgaatc
     3841 aagttctact gtcgaagcgg aactaccaga taaaattgat tttgatcctg aaaaagtcaa
     3901 atcgtttgaa tctgtttctg gttctatggc tgatcggttt aacactgaag tcttcaaagc
     3961 tatcaaaact gttaaattgg atactgaaaa gcaaatgatt aatgttacct tattcaattc
     4021 agttgaatct atggatcagg ataaaaaagt tacggttgca agagaactag gaaaaagagt
     4081 aattggtttg tatcgtggta ctgctaaacc agattcagac ggtgcagatt tgccagttca
     4141 tgtagtttat gaagacggta aagtttttgg agattctaca gcaaacgaag cattaacgat
     4201 taaattggct aaatagccag aaagttataa taaacaaatg gtgtcacacc aataatatat
     4261 aggtgtgaca ccttggagat gatagctgat ggtagtaaat aaagaaaaag ttagaaagta
     4321 tgtgacttta aaaatagctt ctgttaaata tttagaaaag cgagctaaag aagaagaaag
     4381 agcaagaggt aagaaagtaa cagtttcaga gttgttagaa gaattgatcg agaatgatca
     4441 aacaataaga cgtgcattta ctaataggtc tatcagataa caatataatt aataaaaaaa
     4501 gaaagagtga tttggtctta gactaaatca ctctttttat ataaagtttc tttacactca
     4561 ttccgaataa tgttaaaatt ctttatacga tatatagttc cttgtcagag gaagagataa
     4621 ccagcaatat tggagggata ataagtggaa aacaataatg atattagaca ttttagcatt
     4681 ggggaaagag ttacaacagt aagtgaatta tcaaaggatt atagagataa agagggaacg
     4741 attatttctc ttaataagaa tcgaagaggc gtttggcaat atgttgttga tttaggtgat
     4801 ggcgttacag ttgagtgtaa agatagtcaa ctgatcagaa ataatggcag tcattccatt
     4861 gaaaaagcaa ttcttgtata cccaaaagta tcaagtaaga tcatgaatga ctggttgctt
     4921 ggaaaatttg gtgagtacaa taaaaaagaa atcgcagaag tgatcggcaa atcttatcca
     4981 acagtattag ggtatctaaa aaagataacg agtaaagaag caaaaattga ggatgtttta
     5041 acagtaagag agcttcaatc attaatcgag tatcggaata aacaaataac aaatcctgag
     5101 taccttagaa aagaagatgg tctattgaaa caattggaac aagtaaataa aatccggaag
     5161 atgcaaaagg tagctccttt aagccttgat acctttaaaa aaatctattt gaaagagatc
     5221 aaaaaagact aatgtggact gtaactttac cgttccaacc tgtatctgca ccaagaccaa
     5281 gtgcaagaag gaatgctgct gatgaaacta atgacgtagc agagaaaagg atttcaacct
     5341 actatgataa aaagtatctt gattatttga aaaaaataaa gagctttatc gaagatcaag
     5401 atttattaga tgatgaattt tactcgattg taaatgatcc tatgggcgca attatggaag
     5461 tggactttta ctaccagata ccaaaaagct ataaaaaagt ctataacatt atgaagacaa
     5521 cagcgccaga tttagataat ttagtaaaaa gtgctatgga tggtattttt aacatctcag
     5581 ctatacgaga tagccatatc acaggcttaa ttgcttttaa atataatgtg gcgaacgaag
     5641 gaaaaacagt tgtgaggatc aaacgatatt cagaatttga gtgggatact tctgaaggat
     5701 tgaacggaga tatatgggaa gctacatttg attttaaacc agtagcaacg ccaagaccaa
     5761 agtctaaatt tagaggaaat gggattatca cttactatcc aaaagagtac aacgactatt
     5821 tggaaaatat caagaatacg cttaaagaga aagatttggt aaatgatcaa ctgaaaaagg
     5881 taatgaatgc tcctatgggg gttatagctc agatagatta tttttaccaa gtgagaaaag
     5941 ataagaaaaa gcttgattca ctgatgcgta cttcacagcc ggatattgat aatttagtca
     6001 aaggaacatt ggacggaatt tttaacaagg aaataggcat caaagatagc agggttgtag
     6061 ggttaattgc ttttaaatac aatacttttg aaaagtcgaa aacaaacgtg agactacagg
     6121 agatgggctg atgaataata atgaaaaaat tcttcatgta cttgatagct ttgaaataat
     6181 tcaagaagaa ctaaagaaat atcgggatgt tttggaacaa agatatgatt ttgtcaatca
     6241 gcaaaagagt aatcacatgg atttcattct caatatgaat gacttgaaga agaaattagt
     6301 tgaacgaaaa gaacaagaaa agttgatcaa agcttacttt gaactcggtg aaaaagaagt
     6361 taaaaatgca atggaactta atgaagagcg cagggtttta gatcagctgt tagaacaatt
     6421 attggtgatg tttcaaaaag gacgaataga tgaggattta attgaagaag gattgagaaa
     6481 atatccttca aactcaggta tcggaatagt gctgaaagcg attgatgaag aagaaattga
     6541 agctttcata ccgccagagg attttgaatc ggcaatggaa tatataaaat attatagcca
     6601 agggatcact gcttttagag agtttgatcc tgaagatgtt attcatgact tgaacaacct
     6661 aaaagaatgg tgtgaaagtt atggagtcga tgattccgga ttggactact tgatcagtat
     6721 tatggaaatc gaagaagaga tgccggataa gcctgatcca acagatattt tagaattgat
     6781 ccatgaagct agaaatccaa ttgcctatat ttctagagga tatactgtat tggaatacta
     6841 taagccttat atatcagcga tgaaccattt aagacgtgta ttaagggaaa aacgtgaata
     6901 ccgctcagtg ttgaatgcaa cgaatagatt agaaaaagct gtttcagagc tagatgctta
     6961 ttatcgtgaa cattacctgc aagcaggtgg tatgccaaga aatacgaaag caaatatcag
     7021 taggtatata aaaaaagctg agtaggcgga gttagcccat aaaatggcaa aaggtaacct
     7081 gaaaggggca ttttgaatga aaagggatgt ttttgaattg tacgtattag aacgtctaga
     7141 agacggcatg atggcacaag agataattga tgaaattgat gatatgatca gcagatttgg
     7201 tcaattggag ttgaatgatg gaaacaaaaa ttcggtagtg ctaaaaaaat tttggttgaa
     7261 caatttttcg tataaggatt ttatcaaaga aaaggcaaat acattgtact gactaaattc
     7321 acgaggagca gtgatgagag cttattgcta agagcagtta ggctcttttt tgtttgtgta
     7381 aaattatttg ttttataata cacatttata cacaaaaaaa tattgacgga tagtacacaa
     7441 atgtggtagg atatatttgt aagcaagtta gattaacaag ttaaaggaag gaagagcagt
     7501 tatgaaaaaa gaagaactaa tagatatatt tcaaattgtt gaacgagcaa ataatatggg
     7561 aattatgttt tttgatcgta tttctttaaa gatggattta agcgttgccc accaagaatt
     7621 taatctgcga ttaaaagcct tgttgaattc tgatgatgtt aatttcgctc atgatgttgt
     7681 aggtatacag aaccatatag atagagaaaa caaacgtatg ggtgatgaat tcttaccaag
     7741 atattcaagt ttatgatttg gcgaaagatt caagagtatg tgtcattatt gataggtact
     7801 cttgaattgg aaaggaggaa tgaatttaag acaacaacga ttatgatctt aaaacaatcg
     7861 tttatgtaat tggcaatctt acgaataatt ttcatgcaat aatgacaata aaaaatgaaa
     7921 aggggtaaaa tcaaatgaaa aaatttttag taggaattct attcacaatg gggctagtat
     7981 tattatcacc ttcagtttca gcagaaagct ctatttccat gtatcgtatg tataatccca
     8041 atagcggaga gcatctatac actagaagcg ttggagaaag agacaatctg aaacgttatg
     8101 gatggcatta cgaaggtgtc gcatggcaag ctccaacaag tggtactcct gtttatcgtt
     8161 tgtataatcg gtataacggt gaacattttt acaccgttga tgccggagaa agagataaga
     8221 tttcacacac aggatggacg tacgaaggtg ttgcgttcta ttcctacaac ggagcaaata
     8281 gagttcctgt aacacgttta tataacaaat acgtgaattg gcatcactac acgttagatc
     8341 aaaatgaaaa gaacgtaatt agtaaacaag gttggaagat tgaaggtaca ggctggtatg
     8401 caatgccaaa cagtgtacca aatcctacac ctgctccaag cactgaaaaa gtgttgaacg
     8461 ttcctgttgt atggcaagga aatacaatgt tatgcgaagg agcttcttta ttagaaggtt
     8521 tacactacaa aggtgtagcc acaaatcaaa gcttaaccag ttttgtaaac tatatgccaa
     8581 aagctaatga caataatcct tatcatggtt actcaggaga gtggcgtcat aacgttaatg
     8641 gaacgtatca aggcatgatg gctgacccag tagttaaatg ggcaaaaaac gttggcggta
     8701 atgccgtcaa tatcactggt tcaggaacga atggaatcaa gaatgaaatt cgcaatggta
     8761 atccggttgt aacttggatt acttataact atgctacgcc agagttcaaa caaatgcctt
     8821 ggggtcgtgc agtatggaat ggacatgtag ttctcgtgga tggcttcaga aatggtgcat
     8881 accatgttgt tgaccctgta tttggcgcaa aatggatcaa tgcaagcacg tatgagagag
     8941 catttaatgt gactggaatg gcggttgctg tacgttaatt gatagaatta tcaactaaat
     9001 gaaataagag cttaattact gaaatggtaa ataggctctt atttttttag aggaaatttt
     9061 tgtaatacga atctataaca aaaaatattg aatgtagtac acaaaagtgg tatattaaaa
     9121 tcgtagagat tatgcgaaga ggtgattttg atgaaccaaa gaagaagaaa aaggttagaa
     9181 gaaataagcg agcgactttc cgagctaaag gatgagttag aagtaattca agaagaagag
     9241 tacaaagaaa atatacctaa aaatctacag ggatcagaaa gatatgaaaa agcagaagaa
     9301 tccgttgatc aacttgaaga agctgttgat ggtttatccg acatattaga attgattgaa
     9361 gatatagcta gctagagatg attaatgaag atttcaggag tgagcaaaaa atgattgata
     9421 tttcactgat agagaagcta cttttttcaa ataagtacac agccaaagag ttggctacaa
     9481 gatggcaaat atccgaatca gctattaaag cctatcgtca aaataaggga agcgtaggat
     9541 ggcgtgattg gaaaggaatc agcattggaa aagcagaaga cataatgaaa cgtgaaggat
     9601 ttcaagctga caatgattct aaaaaagttg aatagtttaa tagtcgccaa gaaaagtacc
     9661 aggacgaggg gaaaggtaga ttacaaagga aaacactttt aaataaactt cgcaaaagca
     9721 tcaggttatg agtggctagg gctatacgaa tgtgatgaaa acaaagggct ttaatgtcaa
     9781 agaaaacgca ataggggggg aacaaaattt ttaaaaaaga tatttatgtt tggtttaatg
     9841 gaaaaaaata tatgactact aacaaaaggt ggtcagagct agtacaaaat acagttaaaa
     9901 aagtcatgcc agatggatat catttcaact atatacaatg ctcacaaaca actgaaagta
     9961 tttatttttc aatattaaat gagaatagac tctattattt cagggtaagt tcacaccata
    10021 ggaaaaagag agaagataat gttacgacat ttttatcggg aaaatacaat ggcttcaaag
    10081 agttaagtga agccattggt gagtatctaa aaagtaatag atttgtcaga atttctgaca
    10141 aacatatatt gatcttgtta ctttatttga ataatagctt caaattgttt tggagagacg
    10201 acactttcta ctatattgag aacggcatgg atatttattc tggtatcaac ggcatattaa
    10261 aacattatat taaggcgtta cttcgatatg gggttcttta tcaagaagat ttaagtaacc
    10321 aaattaagat tagtaattct ggaaagtcat tactggcaaa atataaaaaa ttgctttctc
    10381 ctatctacaa aggaatagat atggaagctg taaattctga aactttattt aatttagttg
    10441 ttgatgaaaa gtttgcgaat gaatttttta aaagtaaaaa aattgaaaga ctaaataacc
    10501 tgtttcctga aaatgcaata aaagcttatg tagataataa taaatcacga ttatacaact
    10561 ggtttattcc caaacggttg cgaggaaaaa tcaaaattgg cgatcaagtg aaagtaatta
    10621 atctaaatca tactaagatc gttaccgtaa cggagttgta tatagctgaa aaagaagata
    10681 ttacaaaaat gaaaccagtt attaaaatac tagataacaa agagtgagtg gaatatatgg
    10741 tagcaaaaaa gaatactcta aaagaagctt ctgaaaaata tcaagatatg aaaagacgag
    10801 gattaatacc aaaggacaaa aaaggtatat ggagagaatt tgttaaggaa gtcagtagtg
    10861 atcgaagtgg cggtgacttt gttggacgat atacagcaaa aggttatagc aggcgcttaa
    10921 ggatcactgt tgaatatgga acgaatgaat cagctacaga gctgaagcaa aggtacttag
    10981 atacacgaag agaagcttca aaaaaatatg atgatttcaa gagcagaggt ctaattccta
    11041 agaagaaaaa ggggatttgg cgtgagtttt tcaaagaaga agatagcaat cggaataagg
    11101 gagacttcat tggacgatac acagcaaaag gctacactaa gaatttaggg atcaaggttg
    11161 aatacggaac tggtgaaagt tcatgggaat tgagaaatcg ctatttaaat acattgttag
    11221 agaaatataa agcagaatta ttggagttgt tcgatgttaa tgattttatc gagatggctg
    11281 aaaagtatgg tcatgaagaa ggttttatga tcaatgacat tatggttatt gataatttta
    11341 cggatttatt agcacttgcg aaaaaagcaa gggatttaag agaaacgcag atgtatctat
    11401 tcagaaataa aaacggaaga aaccggagaa ctgaatcgag tgagattgaa gattactatg
    11461 atgcctttac acacgatctg ggagcctttg tagacgagat aagtggtgta atgaacaaat
    11521 atagggtcta gaataaaaaa gccggagatg agcagataaa tgggaaacaa tagcatatac
    11581 acacctgaaa tgcagaataa aattaaaaga cttgtcggat tagggtggat gacaagagga
    11641 aaattagatt cttttagtac gatcaaatat cttttgatgg acatttaccg tcagaacaag
    11701 caaacaggaa tacatttttt gggctatgat aagccggaag atgcaccttt ctttgttcca
    11761 agttcagcct atagagccag tacctccggt agcatgtata acttggattt gtttgaggaa
    11821 ggagcaaaga tgatcgaata tgatttcaac gaagcctatt cgaggatcat gagaaattat
    11881 cctttgccgt caaacactta tttgaaagca aaatataata cagatcaagc tattttgaga
    11941 gcgataaata gaccaaaagt aagaccgtat gaaaatttaa aaacgtatat gttctttttg
    12001 gttaattttg aaggaacagc caaagaagga acatatgcta atccaaaatc acagtcgatg
    12061 tttgcggatt atggcgaaaa tcccaaaaca gctaatgagc ctatttggtt atcggagatt
    12121 gagctaacac ttatccttga ttattatgat attaaagaga tggaagtcct agatagccac
    12181 acatttttga cgaaaaaagg attactcaaa gaatattttg aaagaatcga gccattaaaa
    12241 actgatcctg atacgttgaa gctttataaa aatcttagaa cgaaagtatt tggtgctatc
    12301 ggacaaaaat cattaagaga aggagaaaca agcaccttcg ataaatacag tatgtataat
    12361 cgagcgtatt cctccatggt agcaggcgta tttagagatc gtatggttag atatgaacag
    12421 aaatacgttc atagcgcata tgggctatta aaaattcgta ctgatggatt gtatttcaga
    12481 tccgaagtac cagagtttga agagctgtat aaacttggag tagttaagaa aaaagttcat
    12541 acaatatcca aagaagaagt cgaaaaagct ttagaattta aaaacaaagg atttgaaaat
    12601 aagggctcct ttgggaaatc taaagatgaa gttaagggtg ttgtgataga cgatgatttc
    12661 gagtttgatt ttgactaaat actctttgga aaagatggta ggaggtaaat atgaagaaga
    12721 aaattgtgga aggaaatgta ggtgttatta ttctaaaaga ttcaactgac tatgctaaat
    12781 caatggcact aaaagacgaa aaagttgcac gcatggataa agatttagtt gaatttctga
    12841 tggatgatac agaaagatta aaaggggatc agtttttgag tttaatgaaa gacaaaggct
    12901 taaacacata tggtttattt gattataaga aattagctat agagtgggta aaagaaggag
    12961 aaatattttt tattgaagaa tgggaaggta ctgagaactt agttcttcat cagtatcttt
    13021 tgaaagctta atctaagtaa aacagttctg actagttatg cttgtcagaa tttcaaagga
    13081 tgttttccat aaccatcatc attcaaaaat aaaggatttg aaaataaggg ttcctttggg
    13141 aactctaaag atgaagttaa gggtgttgtg atagacgatg atttcaagtt tgattttgac
    13201 tagatttttt tgaaaggttt aagagtaaac aaggtaagaa ctaagatcat gaagattgag
    13261 agtaaagaag atagaggacg aatgtatgag aacacactat atttatttag aagataaaat
    13321 tgtagatgaa attccggcaa aaagtcgaga acaagctaga aaattattta ttgaaaaata
    13381 caaagagatc aatggagata gtgaagttcc aaaagtggtc gtaacagtta gaaaaaggca
    13441 aacaaaaaag agaagtacgt ttcatgtcag ccctgatata tataatctta tagagcgaca
    13501 tagaagtgaa gataatattt ctatctctca atatattgct acagttattg atgaattgat
    13561 ttcaggaaag tatgactatg cttttgattt tgaggatgaa aaggattaca aaagtgttgc
    13621 tgtctttcaa tctgattttg agaagctaaa aaactatgct ggcaggagaa atgttaattt
    13681 tgcaagacta ctagaagagt tctactccga agagttggga gtaggaaatg aatacaagag
    13741 agaaacttat gcgaaattgt ctcataagtt atcccaagcg cttaaagaag tgacagaagt
    13801 taaagcggaa ctagatgcta aacttgaaga agtgaaaaaa tatcaaacag agaggaagaa
    13861 caacatgaag aatcaagtgg aatttgacga acagaaagaa tttgaaaaag caagaaaacg
    13921 ctataacaaa attatgaatg aacttagcta tgaacactta acagttcatg aagaattgag
    13981 tgagggtaca gaaaaatata cgatcgcaga tatggttaat gaagtggaaa ctcttttagc
    14041 ttctttcttt gaaaatggtc acgtaaacga tgaatggcga aaagaagaac ctaaaagggt
    14101 taacgcttat attgctcgat taaaaaggtt tattgataaa tataaggaga atagtgacaa
    14161 attttctaaa gataaataag gagagagtgt gattacattt gccagaaatc gaaggaatat
    14221 tttcgccaaa aataggtaaa aaacagttag atttcagcag ttttccgagc gtaaatagag
    14281 attataatct aggtgatgag gtacagatac taaatattga tttcaaagaa ctagacttcg
    14341 ctgttgctgt ttccaacaaa cccaaggcat tggaacgagt gaaaaaaata taccaagaga
    14401 atgatcaaaa tatcaaaaat atgatcaaaa agtatagacg agataattca aaagttgaag
    14461 acgttttatt caacggtacg gcaaaacatg atttgaatgt ttatatggca ttaggagtgg
    14521 ctttgtactg taattcaatt aatcagacag atattttggt taatatcgtg aggaatagct
    14581 atcaaaaaat attaaaaata agtagcgaaa caaaccctta ttatgcaatg aaatatcttg
    14641 gggataattt attaaatgat aaaaaattga tgcacaacac tgaagagctg ttatctaatt
    14701 tggctgcgat taactttcta ttcaaaacag gttctgaaga ttattttata gataacgtac
    14761 taaaaggtgc ttacggaaca gcattttatc cgctttacaa tatgaaacag cataaagccg
    14821 aacaaaagga taacaaactt gaaattaaaa agatgaaaga actattaggt ataggatttg
    14881 taaagcccaa ttcaaaagtc actattgctt cgttagcaac tactaacaat gatgggaaaa
    14941 tagtaaatgg gtttgacgat tcagatatag atatattgtc aaaatcatat ttttctttgt
    15001 atggagattt tgcagacttg atgggtattg atagttactt agtcaataat tttgagatca
    15061 agggtagcga gctgaatgat attgtgtatt tcgttttaat ctctctatat gcaattgatc
    15121 agaataccga gtaccgacaa attgactatt tgctagtttt ttatagtttg ttatatcagt
    15181 tcgttttaac tcggttgttt aaaaatgcta aggaagaact gatcctgaat tctgattatg
    15241 aaatagcaaa acaaattgca agtattagaa aagaaaacaa atctgaagta atcaaattaa
    15301 gaaaagaatt aagtattgcg agtaaagaaa acggtcggtt agaatcttta gtcaaatcgg
    15361 caaaaaaaga agaggaactt ttgaagcaag agattaaaga attaaaagga atcagaaaag
    15421 aatttgaaca gctgaggttt gaaaataaag tattaaagca attgcaggaa caagaacata
    15481 gacaatcatt aaatggtaaa actgaagagc cttcactgtc tgaaatgata gcttatctaa
    15541 aacataaaaa cattctttta gtgggaggac atgtaaattt tcaaaataaa ttaactgaac
    15601 tattaccaaa tattaaaatg tttgatgtaa gcgaaaataa tggaaagata cttaaaaatt
    15661 tgaatacgtg tgatgcgatt tttctatata cagattactt gaatcatggt ctatattatc
    15721 gtgttatgag tgaaatttct aataaagata agatatttta tttggataat catgtgaata
    15781 tgaacttaac aattaaacgt atttacgatc ttttatcact tatataaaga gagaagtgat
    15841 aaataggaca caaaatttgg aaagtaggta agtcactatg agagaactaa ctttatcttc
    15901 tgaggaatta gaaaagaaaa ctgctttatt aaacgaaata tttagtgctg attgtataat
    15961 ccaaagttta aaagaaagtt cattttcaaa actaacagat cagaaaaaaa tttcttatat
    16021 gctaagttta gataatctag gaactaaata tgatgaatcg gcaagtagcg ataagctttt
    16081 gattgaatta gtatcaagtc ttagtagttt tggaaaaatg aaaagattta atgtgatatt
    16141 agaaattttt gcttggataa tcccttcatt tatttggttt ttttatattt tcatagttga
    16201 atctgagtta atagccaagg tctctcttga actgttaatt ccgatatatg cattttattt
    16261 aataggttca gcaatttact ttaataactt gtatacacgt attcaaataa aaaagcagtt
    16321 attacatgaa ggattaagtg gtaatatgtt aaaaagaatt gcatgggata aagataataa
    16381 aacagttatt gagcttaaag aatataaata caattatttc tcgagatgta tagatagaaa
    16441 gttactgttt aaaaaagaag gattagaatt tagttttgat gaagatgaat ttgaaaaatt
    16501 agtgatggac tggcgtaaga atttactgga tacggtacaa ttctaaaagc aattgaggtc
    16561 gaaattgcg
//
LOCUS       DACDIE010000053        16191 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.053, whole genome shotgun sequence.
ACCESSION   DACDIE010000053 DACDIE010000000
VERSION     DACDIE010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16191
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.053"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..466)
                     /locus_tag="IV250_002067"
     CDS             complement(<1..466)
                     /locus_tag="IV250_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAP8769324.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANF"
     gene            complement(596..1306)
                     /gene="budA"
                     /locus_tag="IV250_002068"
     CDS             complement(596..1306)
                     /gene="budA"
                     /locus_tag="IV250_002068"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HAP8769325.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(1319..2983)
                     /gene="alsS"
                     /locus_tag="IV250_002069"
     CDS             complement(1319..2983)
                     /gene="alsS"
                     /locus_tag="IV250_002069"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAP8769326.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(3188..3850)
                     /locus_tag="IV250_002070"
     CDS             complement(3188..3850)
                     /locus_tag="IV250_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP8769327.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(3860..4675)
                     /locus_tag="IV250_002071"
     CDS             complement(3860..4675)
                     /locus_tag="IV250_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8769328.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(4937..5380)
                     /locus_tag="IV250_002072"
     CDS             complement(4937..5380)
                     /locus_tag="IV250_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HAP8769329.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(5514..5852)
                     /locus_tag="IV250_002073"
     CDS             complement(5514..5852)
                     /locus_tag="IV250_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8769330.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(5840..6217)
                     /locus_tag="IV250_002074"
     CDS             complement(5840..6217)
                     /locus_tag="IV250_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP8769331.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKEIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            6441..7634
                     /locus_tag="IV250_002075"
     CDS             6441..7634
                     /locus_tag="IV250_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8769332.1"
                     /translation="MTTYQRKVLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(7800..8222)
                     /locus_tag="IV250_002076"
     CDS             complement(7800..8222)
                     /locus_tag="IV250_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8769333.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            8617..8811
                     /locus_tag="IV250_002077"
     CDS             8617..8811
                     /locus_tag="IV250_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769334.1"
                     /translation="MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQE
                     QIEKKKEEKQKAEKAALEKK"
     gene            8808..9302
                     /locus_tag="IV250_002078"
     CDS             8808..9302
                     /locus_tag="IV250_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769335.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQSA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            complement(9464..10636)
                     /locus_tag="IV250_002079"
     CDS             complement(9464..10636)
                     /locus_tag="IV250_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8769336.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(10842..11657)
                     /locus_tag="IV250_002080"
     CDS             complement(10842..11657)
                     /locus_tag="IV250_002080"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP8769337.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(11807..12253)
                     /locus_tag="IV250_002081"
     CDS             complement(11807..12253)
                     /locus_tag="IV250_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAP8769338.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            complement(12250..13254)
                     /locus_tag="IV250_002082"
     CDS             complement(12250..13254)
                     /locus_tag="IV250_002082"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP8769339.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            complement(13294..13623)
                     /locus_tag="IV250_002083"
     CDS             complement(13294..13623)
                     /locus_tag="IV250_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAP8769340.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            complement(13749..16076)
                     /locus_tag="IV250_002084"
     CDS             complement(13749..16076)
                     /locus_tag="IV250_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8769341.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
BASE COUNT         4621 a         3240 c         2870 g         5460 t
ORIGIN      
        1 taaaatttgc taataaaaga tctacatatt tttctagaaa tgcttgatcg atttcatcat
       61 accctttcgt tactccgcaa tcgatatcta aaacaccgat taaattacct tcttttacca
      121 ttggcactac gatttctgat tgtgcggctg aatcgcagga aatgtaattt gcatgggttt
      181 tgacatcatc aacgatcaat gtttcttgtt tttcagctga ttctccacaa actccttttc
      241 ccatcttgat acgtgtgcaa gaaactcgcc cttggaacgg tcctaaaatc aattcattac
      301 catcgaataa ataatatcct gcaaagacag tctcaggcaa agtctcattc aacaatgcag
      361 atgaatttgc taaatttgct aaccagttat cttctgcttc taataatcct tgttgctgta
      421 ataataaaat ttcgtatgca tcttttttct gtttttctgt ccacatgata aaaaccgctc
      481 ctttttgatc tatggagaag attatagctc agtttttttg taaacgctag gtttcctcct
      541 caaaaaaacg agatacgtca ggttaatcac cttgaacgca tctcgctgtc tttttctatt
      601 ctgcttgttg gatttctgct gctaaatcag cataatcaat cgtttcgtga acaaaagtcg
      661 gatcatcaac tggaaaatgc tgtacaaggg acgagatgcg gctaatctca acttttcctt
      721 cagccacttc aaaatccatt acatgtccgc caaatgtatg tgtatcatcg ataaaatgca
      781 agtgaaatcc cgctgcagca actccttgga ataattcagg cgtaaagaac ccgactaatg
      841 ttccttggac attgctttgt tggaattctg gttgagactc tgaaattttt gctagtcttc
      901 tgtaaggttt ttcttgcttc ggcatgatac ggatgtgcat atttttgaat aatccagtta
      961 tcttcactgc caaaaatagg ttttccccgg cttctcttac taatatgtct tttttcaatt
     1021 gttctccatc tgcagttttt gagacagaaa aactagtatc tggcgtaaaa tctgtaattg
     1081 ctgcataggg ggtaagttca cttccatcta attgggcaac tgagccatct gatcttcctt
     1141 gataagctac gccatctaaa aagatgactt caccatcaag cccatgcaat gttcccaatc
     1201 ccagagttcc tttttctaat atatgagcaa tcgtttctgt tccatccatc aagccagcca
     1261 tcaaagctcc taatgtcccg tgctggtata agattttttc agtcatgtcg acacttcctt
     1321 aatagaattg atctggtaag attgtttctc ccaatttctc attatcacgg taatcaatcg
     1381 gaatatcaat aattacgggt ccttctgtcg caaatccttc ttctaaagct ttttctaatt
     1441 cctctacact tgtagctcgg attcctttag cgccaaaagc ttctgcatat ttcacaaaat
     1501 caactggacc aaagtctaca cctgacgccc gctgatattt cattttttct tggaactcta
     1561 ccatattgta gtgtccatca ttccaaatca aatgaacgat attcaatttt ttacgtaccg
     1621 ccgtttctaa atcctgggca gagaataaaa atcccccatc gccggaaact gatacgattt
     1681 gtgtatttgg acgcaccaat gcagcagaaa tcgcccatgg caatgcaaca ccaagcgttt
     1741 gcataccatt actgaataac aaatgacgag gttcataact tctaaagtgg cgtgccatcc
     1801 aaatataatg actacctaca tctacggtaa cagtcatatc atctgttact tttgactgaa
     1861 gcgtgttaat aacttctaaa ggatgcaaga tacctgcttc tccttttgat tgaacaatgt
     1921 cccgctccgt taattctgct tgaagagatg ctaaatattc ttttgcatct tctgacactt
     1981 gttgaggctc taatgaacct gtcaataaat caagagtggc tgagatatca ccgatcaatt
     2041 ctctttcagg ctgcataaaa ggatcgattt cagctggcgc ttcatcgatc acaatgatcc
     2101 gcgcatcttt ttccgcattc cagtttcttg cttcatattc gattggatca tagccaatag
     2161 caataactag atcacttcgt ttcaaaagca tatctcctgg ttggtttcta aataaaccca
     2221 cacgaccaaa gaaatgcgct tctaattcac gtgagatcac tccagctgct tggaatgtct
     2281 ctacaacagg caatgctgtc tttccaacta attgcctgat ggctaacgtt tctttttcac
     2341 ttgatcctcg catacctact aatagtaccg gtagttttgc ttctctaatg cgttcagcta
     2401 ggtaacggat atcatctgca gaagcagaac ctaatttagg atcgctcaat ggcttgatga
     2461 cattcccttg gacaggtgca tccactacat cttgcgggat actgataaag ctcgcgcctt
     2521 tttttgaact cttagccata cgataagcat tggcaatcac ttctgaaatt gtttctggat
     2581 cttggatttc tgcactgtat tttgtgattg gctggaacag tgctgcatta tccatgcttt
     2641 gatgggtcaa cttcaataga tcgcttcgtt ttacttgtcc ggcaattgct aaaactgggt
     2701 caccttctgc tgtcgctgtt accaaacctg tagctaagtt actagcacca ggaccacttg
     2761 ttgcgataac tactcctggc tctcctgtaa tacgtccgat tgcttgtgcc ataaatgcag
     2821 cattttgttc gtgacgagtc acgattaatt caggaccttg atcctctaat tcattaaata
     2881 caccatcgat ttttgcacct ggtataccga atacataatc aacatgatga ttaatcaaac
     2941 tttctactac agatttcgaa ccttgtttca ttttttcgtc cattgaacat cgtccttttt
     3001 atgctttatt tttaattatt agttagaaat aatcacaaat aattgataca gtttctaaac
     3061 ctgtttcatt caaaagtata gcacgtttta tttcatttgt ttagccatat gtttcaaaaa
     3121 atgaaaaaga atgacagttt ttacataaaa actgtcattc ttaattgaaa gtttcaattt
     3181 ataaagtcta cagatccata gttctcatca atttacgatt gttgaagtaa atcaaaccaa
     3241 atacgataga aggacctagt agaatacacc attggggctt tacgaaaaag aagagaaaca
     3301 aacttgtcaa tagtaaaaaa tctagtaaac tcacatacca ttttttcaac gtatagtaga
     3361 acgctatccg tagaacttga tttggtttcg acgctggatt tctcacctga aaataccagt
     3421 agttgatgct aaaggaaact gataaacaag caagtaaaac aaacaacgga atcaaccatt
     3481 ttccataaga aaaacgtata aaaaagaaac aatcaaaaat cattatcaca ctaactatcc
     3541 aagcaaatag ccaataaaaa aatcccttta gccaaaattg gcgaaacgtc tttatgaaca
     3601 aacgtactgg tgaaaaactt ttttcttttg ctaattcttc tgtcatacct atcatcacga
     3661 tgcaacctgg accaataaaa agtaaagaaa aaataaagga aaagacattc cttccatcta
     3721 tcgacaaaag gaacgcagct gctatataca agatattgac acaccaaaaa caaaggctac
     3781 ttgccaatga ttgatacaac caataaaaga attccatata aacatttttg tcaaaacgct
     3841 gctctttcat aatcacctcc tattccttca ttcctgatga agcgatccct tctacaaaat
     3901 atttttgcat cgctaaaaat ataaaagcaa caggaagaac agaaacaact gaggcagcca
     3961 tgattaatgc gtactctgca tcaaatagcc caataaacat tttcaaccct aactggattg
     4021 ttttattttc cgtagaagac aaataaatga atggccccat ataatcgttc catgtattaa
     4081 cgaatgtgat gatcgtcaaa ctggcaatcg ccggttttgt aagaggtaaa atgatcttgc
     4141 gatagattcc ccactctgat aagccatcga tccgcgcact ctcacataaa gaatctggga
     4201 tactactgta gtattgcttc aacaagaaca caccaaaagc attgaacgct tgtaatagca
     4261 ccaacgacca caacgtatat gttaaaccca cttttctcat cataatgaac tgcgggatca
     4321 tataagactg ccaagggaca gcaatcgtgc cgatatatgc taaaaataaa atatcgcgcc
     4381 ctttaaaatt tagtttagaa aacccgtaag cagcaaaact agaagtaaac aactgaatca
     4441 acgtgatcac aacgcttaaa aaagctgtat tcttaaaaaa tgtcactaaa ggaatacgag
     4501 accaaataac cagataattc tctaaatgcc aagttttagg gatccattga atcggaatcg
     4561 tgaacacttc attatttgct ttgaatgaag cagaaaccat ccataaaaaa ggaatgatcg
     4621 tcaataaagc taatacaatc agcagaatca tacccaacca ttgaataaag ggcatgtttt
     4681 ttgaacgaat ttttgctttt tttctttcat ttcgaacacc tccaaagtta taatcttcat
     4741 ttctttgtaa tgattacaat ccagaacaac ggttattata gctgattcat ttccgttctt
     4801 cttatgtttg taagcgctta tttaacaagt tatgtaaatc tctttatttt ttttgattta
     4861 aaaatgaaaa aatacaaaag caagactgtc atacgtctcg cttttgtatt gaaatttctt
     4921 cgattagaga tacttattac tgttcaaaag cttctttgaa acggtgaatc aattcattga
     4981 ctacaggtga taaaatccga ttctttttcc agactagcat gcagttcgtt tcaatcattg
     5041 ggtcaagcgg aacgaatgtc actcgctttg aaagatgact tacagcacct tctatgacta
     5101 aagcagcacc tacctgattc tcaattagcg caaatacatt gaaaatcaaa ttgtacgttc
     5161 ctgtgatcag caactgctcc ttttttatgc ctaaccagtc agcaaacatt cgctgtattt
     5221 ctggacgctt tgagatcaaa agaggtacat tcgcaaagtc ttcttttgtt atcacgtctt
     5281 tttttgctaa aacagaattt tctaaaatga gcaatcccca tttttccttt cttggtaaca
     5341 aaatcgtttc gtatttctct gtatctactg gatctagcaa aatcgctaaa tctaacaggc
     5401 ttttttctag tcactccgtg atatcatcac ttgttccgct gtggatatga aattgaacgc
     5461 tgggataatc acggatcaac ttttctagta atagtgccaa tgtattcgaa ttatcagctt
     5521 ccacacagcc gatcgtaaat cggccgctga ataattgttt ttttctgttc tcgaattctt
     5581 gagtggtttg attcgttaaa tctaaaattt cttctgctcg gcttttttag aaaataacct
     5641 gcttctgtca agatcatttt gcggttttcg cgtacaaaca gttctgttcc tagttcctct
     5701 tctaactcct tcagttgtcg gcttaaggtt ggttgtgtaa gatgtagaac ttctgcggct
     5761 tgagagattg ttccttcttc tgcaattgtc caaaaatatt ttagtaagcg taactccata
     5821 gcgatgctcc ttgatttttt tacttgatac attccatttt atttctagat aacacacgat
     5881 catatttctt gatttttcct tgtaaacgtt ccaaacactc ttgcatttca gcaatttctt
     5941 tcgtcaaaag ttcactttct tcaatcaaaa gcttacgccg ctcaaataca gttgcttctc
     6001 cttcgctaga taggcgaata tattcaatca aagactcgat cgataatccg gcatttcgca
     6061 tacagatcgt aaagtcgacc cattttaggt cttcttctgt ataatcacgg attccgcctt
     6121 ttgtccgatt aactggtgga atcaacccga ttcgttcata atagcgcaat gtatcggccg
     6181 aaatgccctt ttcttcactg acttgtttga tattcatctg atcactcttt ctcttaattt
     6241 cttgccactt tacttatgta ttttatttat tctacaactt taagtgtact ccaagtcaat
     6301 actcatttct attaaaacta agctggatta tctatatcgg ttgtttgtgt attatttcaa
     6361 ataaaaaagt cttgtttttt tattcttttt tggatatact tttaacaatt tagggaaatt
     6421 ttaggaaaag gggtataaaa atgacaacgt atcagcgaaa ggtattatta tcgacatcgg
     6481 cgggaattgc tttagagaat atggatatta tgtttttagc tttttcatta tcttcaatga
     6541 ttacttcttt tcatattagt ggtacgcaag cgggtttcat ttcaacgatc actaatttgg
     6601 ggatgctcat tggaggtatt ttttttggca tcatagctga taaatatgga cgtgtgaaag
     6661 tattttctca aacggtcctt ctgttttcga ttgcttcttt attgatgtat tttgcttcaa
     6721 acatctactt ggtttatctc tttcgcttta tcgcaggtgt aggagcggga ggagaatacg
     6781 gggcgtgtat gtccttgatt tccgaaagtt tttcaaaaaa acaaattgga cgagcttctt
     6841 cggttgctgg tattggtgcg caggtcggtg cggctttggc tgcgatttta gctgctgtga
     6901 tcattccaac gtttggttgg aagatgttgt atgtcgtagg tgtactgccg gtgttgatgg
     6961 tcttgttcat tcgtcgcggc ttgactgaac caaaggaatt caaatctgta caatctgccg
     7021 aaaagaaaac caaacttagc agactgtttg aatcaaaaca tcttgcttgg caaacatgtg
     7081 gccttagtat catggtaact gtccaaattg ccggctattt tgggttaatg aattggctgc
     7141 cttcaattat gcaaaatcga ttagggcttt cggtttctgg gtcttcattg tggatggtca
     7201 gtactatttt aggcatgtct ttggggatga tgacttttgg cataatcatg gataaattgg
     7261 ggcctaaact agccttttct ttgtttttaa tttgttcatc cttatctgtc tatctacttg
     7321 tgttagccca tagccagtgg agtttggtag cagccgctgt gatagtcggc tactttatca
     7381 atggcatgta tggtggatat ggcgcagtga ttagtagttt gtatccaaca gaaatccgcg
     7441 cgacggcgaa taatttcatt atgaaccttg gacgagctgt tggtggtttt tcttctatcg
     7501 tcatcggttt cttgatggat cattatagtt taactgccgt aatcttgttt ttgagcggta
     7561 tttacctagt cagtctgctg gtcttgacaa ctcttactgg tgttacagca ttaagggaaa
     7621 gcttgcagca ttgatagttg agtctgtaaa tgacaagaaa gaggtgtggc aaaagcttgc
     7681 atctgcggta ataagaccag ccaaatcaaa aacatgagac actgtttccg tttggctggt
     7741 cgtattactt gatgcagggc agctttttca actgctaatt tccttctatc tggcggcgac
     7801 tattctttga cgtccgtcat ttcaaagtac tttactaata acttagcata acgaaaaact
     7861 gtggatacac tcgtatattg agcgtctgca taatcttcca aatttttctc aggatgttga
     7921 tacatgaatt caatcaaatg ataattatat gattttctgt agaaatgaag ataaataatg
     7981 tagattggga agttctcctg cttttccata gaaaagcgcc gattattctc attcttcaaa
     8041 atgaccttat caggatattt ttggaacaat tcttctaaat ctgttagata attcaagata
     8101 gacttacttg ataccttcaa ttcgttttgt agttcttcta ttgaacacca acgttctgtt
     8161 tcaagcagta aatgaatcat tttaattttc ttttgagaaa cttttgttaa aaaagtctgc
     8221 atattttctc ctcctgtatc tactttacta tactacatac aagaaaaaaa acggatacaa
     8281 atatcatatt gtcacaccgt caatagtgca taaacctgtt attatttctc tttttttcct
     8341 aagtattcta ttgatatgaa tcatttaatc gtttattata taaatgtcaa taatctaata
     8401 ataaaataac gtacatattt tattattgca tacttaataa cagaactata gcaaaaaatc
     8461 ccgtgtatca acacgggatt agattaagtt tcctcaatca ctattcataa cacaattatt
     8521 ataacaagta agtaaacaat tgaaaaggac atttattgaa aacggttgtt ttttacttaa
     8581 ggaggaaatt tagcgaaaaa attagttgca ttgtttttgg tcgcaggaag tttgttgagt
     8641 gggtgtggtg ctactacgtc tactactcaa agcacaagtt cagatcaggc ttccagcagc
     8701 agtaccacga tctccgaaag tacggcagat tcatcgaaag aacaagaaca aattgaaaag
     8761 aaaaaagaag agaaacaaaa agcggaaaaa gctgcactag aaaaaaagtg acagaagcgg
     8821 aatctgcttt gtcacaagct gaagcaaatc ctacaaaaga aaactatgca actgcttcag
     8881 cagctatcca agcgctacct gaaaacaaac aagagttgac aagccgtctt gctgcaattg
     8941 attccacaat caaagcaaaa gaagcagaag aagaacggaa aaagcaagaa gaaattgcac
     9001 gacaacaagc agaacaagcg caacaagaag caatcgcaca gcaacaagca gaacaggcga
     9061 ggcaagaagc aatcgcacag caacaagcag aacaggcgcg acagcaatca gcagctgaac
     9121 aggcctctca gcaggcacaa gccgcagctc ctgagcaaaa taatgaacaa actgtctatg
     9181 tcacaccgac cggttcaaaa taccatacac ataaatgtgg aaatggtacg tatacccctg
     9241 ccactttaag cgaagctcaa gctcgtggtt taaccccttg ctccaaatgt tatggaaact
     9301 aattcaaaag ttatactata ataataaaat tcttctgaaa cataaaaaaa tagaagatgg
     9361 cgttccagat agtaaaccaa aaataacagt ttacacgtcc atttcgtcag cttctctctt
     9421 ttttcattta gtctgttccc tctgcttttt tggacatctt cattcattct ttctcaatca
     9481 aatagtacac ctctttttct tttcgcagtt tatatcttcc ttttttcaat ttcattgaaa
     9541 actgtttcga atgtttatgt tttacatgga ttttaagcag gagctcctca tgttgcaagc
     9601 gatagttcct gccttttaaa tcagaaaatc tcactagtcc gtattcatca gaagctttca
     9661 caaatggaac tttttctcta gtaatatagt gtttgccgtt gagcgttttt acagaaaaga
     9721 ccattccttc gattggtcgt ccgtttgtgt tctttacata aaaagataaa ggatagtttc
     9781 gcttagcgat ggccaaagaa tctaaataag cgatgagtag ccaaactaag ccgattgatc
     9841 caagtagtat accgataaac caaataaaaa atttccattt tttccattga tcaattcctt
     9901 ggatcgcttt ttttgcctct tttgcttgat aaggaatacg atgccccgtc acaagcagtc
     9961 gatgtgtatt gaccatataa ggagtacagg taagcaaagt cacgagatct ctgccttttt
    10021 ggatacccaa agaatctact tcatcaggta agaccacttg gattttttct acttgatacg
    10081 ccaatgtctt tccattgatc tggatgaaaa actggtctcc ttttttcaac ttaggcaaat
    10141 ctgtaaataa ttttgcttct gggagtccac gatgcccaga aagaacggca tgggtgtttt
    10201 tcccgccagt cggataagaa ctttcttcca ataatgacgt tcctttttgc aaaaatattt
    10261 ccgttgtctg atcgaatata ggtaagctga cattgatttt tgggatttcg atgactccaa
    10321 gcatatgttc cataaaaaag gcattactag gtaaatcttt taatgctttt gcatcaactg
    10381 cttggttgaa actgcctatc cctggtgata cgttttgctc tgccaactgt tggtttttct
    10441 ttgtcatttt ttcctgctgt ttttttattt cctctgaatt cttattcgat gcttgctttt
    10501 gataatggat gatcatttgc tgggcaagaa aatcattcag tgaatcttct acaaaaggat
    10561 aagaaaagat cccgatccca acgactaaaa tgattgccat gaagatgtcc agtattctta
    10621 ttctgctttt attcatcatg tccacctatt ttctaatatg tatatgaaga ggttgtgaaa
    10681 tcagcgcctt gacctgagta ttagcgaagc aaacggcaaa atagttcctc ctattttgcc
    10741 gtttgttgag cgaataccaa gagaccgacc acttgtcaca acctttctcc ccatttgaat
    10801 cactatcgtt tatttatcgt atgatttttg tttccaatag tttaagcttc tgtttcttga
    10861 tggcgttttt tgaaatagat cactgctcct ccaactaaag cagtacctac caagatgaat
    10921 acgatgatcc ccataccacc agttgaaggc aagattcctt taggttggtt atcgatcgtt
    10981 gtttcatcag aagttgttgg atcatcatct actgtaaatt cagtgtacgc agcttcattc
    11041 ttaggtaatg cgtagccttc tggtgctttt gtttcttgaa ggcagtatgc cccttgtttc
    11101 agcccttcga ttgaaaattt cccttctgaa tcagaaatca atttcacttc ttcccagtca
    11161 gaacctgtga cttcatctga ccaagcatat gggtattctc ctgaggcaag gtttgctatt
    11221 tttttcatta atttaacagt tggatctttt gctcttagaa tagaaaattc tgcatcggct
    11281 aatttttctt gcgtgttcac atcttgtttg atgaagtgga aatcaccgta gttttggaca
    11341 ttttctacag taatcgtgtt atccgtagtt gtattttccc gattgactac accgtttgga
    11401 ttcccaagat tggcttcatc tgcgccatgg atgataccgg ttgtttgttg caattttccg
    11461 ttttttacta taaaagtagt ttctttttgt ggattggtta attgataacc atctggtgct
    11521 tttgtttcaa ctaaagtata attgccgtct attaatccat tgacactgat ggatccttca
    11581 gtatcagttg tgaatgtcaa cgctgactca gcaggatcat agagagcgac accagtgttt
    11641 tcatcaaaac cggtcacgaa ttgattctct gaatttttga tctggaattg cgcccctgct
    11701 aacttatcta cccctgaaaa gttcccagct ttctggattt tgatattgct ttctttgatg
    11761 acatttttag catataggtg aatcgtgtct aaaggtttgt cagggtctag ggtaagctta
    11821 acacgatatt tggcgacgtt tcgacaccgg gctttggtgt ttctacaaag acgtatacag
    11881 catctttccc atcagattta cctggaagag tcaggttagc taatccttca ccagaagtcg
    11941 ttacacttgc gaagggtgta gcgtctgctg gaactgctgt gtctttactt ggatagtacg
    12001 tagcagtgat gtcatatacg ttgaattcaa cgccgttcaa cggttcgcta ttcggaaatg
    12061 tcaattccgt tccgttgttt atttgatttt ctggtgctga agtgaatttc aatttatgca
    12121 aattaaccgt aacattttct ggtgtaggtg atacggctgc agaaacctct gcagttccta
    12181 ggccgagaac gaaaggaagc agcatgatag cagttcctaa aaacgtccat atttttgttt
    12241 tgatgttttt cattttattt ccctcctatt gtttcctttt tgttgttttt tataaaacca
    12301 agctgtccct gttgaaagga ctagcaataa ccctaaaatt gagaaacctc caagcatctc
    12361 ttcgcttgtt tttggaaagc ttcctccatt tttttcattt gatggaccat ttgtctgcat
    12421 ctgaactggc gtttttccat tattcgtcgg atttgctttt ggttcgttga tgatttccaa
    12481 tggtatagga tgaagcggat cataactgcc tttttcaact tcaaagagaa tggttgattg
    12541 actcaatata tatcctttag gcggtttaat ctccactagt tcgtaacttc caaaagacaa
    12601 tccatggaca gaaaatgttc catctttact ggaagtaaaa atcgttgctc ttgaatcatc
    12661 tttggctacc caatgattta tcccgttttc ttgcgctaag tattggtcat tttttttgac
    12721 tagaaattga gcatctgcta gtttttgttt cgtttgccga tccactttga caaattgttt
    12781 gcctcctgtt tggatggcaa gatgtgtttc tactgtttga ttgtcagtcg tcaaacgtac
    12841 atcgttatca aatgtcgttt tttcactgtt ttctttcaat gtcatttggt aacggatcgt
    12901 taattttttt ccagcaaacg aactaagatc ttttccagaa tctgataaga caaaaccatg
    12961 ttttattttt tgtgtgtgaa atgtatgaat cttgtctcca ccaatcaaaa aatcgatcgt
    13021 ttctggttct agccataact gaggatcagc tgtatctttg atcgtatagg ttcctatctc
    13081 gttgatgttc tcaggaattg ttacggtgat gagataaggg actttatccc catctgcaaa
    13141 atctgtctgc ttattctcta ctgtttttgt taatgtgagt gcagcaggta ttttttgttc
    13201 gctctttgga tacaagtgga tggtcgtcaa tttatgatta gatgaatctg tcacaggaag
    13261 cacaacgaca aatggtgctg atttttcttt tacttaatct gaagtcttta tttccgtaaa
    13321 tcggtaaaca gcgtcacgac cttgctcatc tttgtccgat gcactaaaag aagcaatgcc
    13381 ctcttcctcg tctactgttt ttgtcacatt ttctgccagt attttttcac tatcactttt
    13441 ttgtgctagt tttctttgtg cgtcttccac actacttcct tctgaccgca gcttgtaaaa
    13501 ttcatccgag acatcatata tagcgaaagt aacgccgttc aaccctttta ctccttgaaa
    13561 gttatccggg atatttattc cgtcgttttg actgatactt ggcatcttat cgctttcgaa
    13621 cattcgatta tgcaaagtaa atgtaatagg tgattctcta gctgacacag tcggagaacc
    13681 tataaaaaag accagtagga taaaaaagag aacagcaaat atatccacac gtacattttt
    13741 tgtttttttc atcgctgttc acctgctttt ttcttacgat ggataaaata aaagaagcca
    13801 gaaaaaataa agatcagtcc taaaaagaca aagggcagta gacctatccc acctgtcgaa
    13861 ggaagcaaga gagtcggatt gatcgtcaac ttagcggtat ctgttgcgat aatcgtataa
    13921 ttattgttta cacttccttc gcctaaacta atcgttacta tttgtgatcc aatatctgtc
    13981 cggctgttaa ctgttagggt ataatccaat gattcgccta agacaacacc ggtaacgtgg
    14041 agatttccaa ccgaaatcga gtgactttgg gcatcataat taaaggcagc gtcatcgaca
    14101 gtaatcactg cgttagcttt atagatggta taagttcctg atgtgttggc tatatcagta
    14161 aatgacaact tgggatttgc tgctttgact ttgtctattc cgctattcgt tagtttaatt
    14221 gtatagctgc cagcattaac catatttttt gctgattgtg catctgctgt gtaatccata
    14281 ctgctaagtg ccacttgtac tggagtaagc gaactagtga caccctctgc tagcttgaca
    14341 tggattgacg ggacacctgc ttttccatca tatgtcttac tgccactgag ctctaccgca
    14401 ccgctgattt gcttaatcgt atagacatca gatgtctgca cgatagtaaa taaatagttt
    14461 gttctggcag ctagtttagc tagtccgctt tttgtcaatt gccaagacgt ataggttcca
    14521 atttcagtag gcgcagttac ttcaactccc ttttcattca tcgctttgaa atcagttgga
    14581 ttccatgtga tttgatcaat actgagatca ttgtttaaaa gggatgttga ttctgtttta
    14641 tcattaaagg catatttaac gatcggtaca acagaaagac catcatagta cttactgcca
    14701 tttgcattga ttgatactgt tgcatttgtc ttataatata ggatgacttc agaaatctgt
    14761 tttccatttg cgtcctttac gattacattt ccatttgtat ctaaactagc taaaacactc
    14821 attgctgatt ggttctttgt ttctatcgct gaaacagctg taaaggtata tccgttgata
    14881 gtaggcgtat tagtcgtcag gtcttgtgca gcacttccgt aagtaagatt cattatttta
    14941 gaaggagcaa tcatctgtcc aaaatagtct tgatacttaa tagtgattgg tgtttgtttg
    15001 cctgtatagg tcaccgtatc gacgttactt tctagggctg tgaaagaacc gtttgtttgt
    15061 gcaaaagaat agcccgttac tgctgttgga gtgataacca ctttgtcacc cacatggatt
    15121 ccagataatg tagtcccgta cgttccgatt tttgctgctc caccattgac tgtataagaa
    15181 aaagttcctg tccgattcgc attgacctga accgttgctg cttgataggt tggaaaattt
    15241 gaaacatttc ccttaccatc aacgatctgc ccatttttga tcgagtaccc cgtgaatgga
    15301 atatcatttg ctaactgtat accgattgaa aaagtactca tacctcctcc ggcaggaagc
    15361 cctccccatg ttgagggtgt aaaagaataa cttttatccg cattgggtgt aactgaatcg
    15421 atcgtatttc cagcggtagc cacccccata tagatacttt ttgggaatgt gatagtagta
    15481 ccatttactg tggagccaat aaacagatta tggatatctg gacggttatc aataaaagcg
    15541 attgctttgg ttacgccttg taacgcagtt ttatccgcac tcatcgatcc gttaaccgca
    15601 acaatgactc catctttggc atatatcact gtatatccat ctggtatggt atgtccgttg
    15661 aaattcgcag tggccgcaac agtactaaat gtctggcttc tacctgtttc tgaataagct
    15721 ttctcccctg ctgcttttgc tgctgtgatt tctttttctg tcggatcaat cagttgagtc
    15781 gaggatgtgg ttgaggttgt ctcgttgttg ctagactgat tggtgctttc tatggactgt
    15841 atttcagaat agggtggact atttgtctcc tctgtcgttt ggttagcaaa atttgacgtt
    15901 tctgtataaa cgtttacata aatacttgat aagctcccta agaaagtaaa aatcaagatg
    15961 atcgaaatca taatgggatg taagtatttt cttttagata cagctttgag tctagaaaca
    16021 aaatgttttt gacatatacg ttggatttta ttttgagttt tcttaatcat tttcatccct
    16081 cctctcaata aaaaatcatc aaataatcct ttacacgtta ttctaaattt aatttttatt
    16141 attttattga tgtatagaaa gtttggggct ttttttctct ttttattcta a
//
LOCUS       DACDIE010000054        15967 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.054, whole genome shotgun sequence.
ACCESSION   DACDIE010000054 DACDIE010000000
VERSION     DACDIE010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15967
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.054"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(50..277)
                     /locus_tag="IV250_002085"
     CDS             complement(50..277)
                     /locus_tag="IV250_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769342.1"
                     /translation="MKPNIKILDRIFLGRDTEVILIQHEEGFEVSIGIQKLQKPHYCN
                     QLYKNFTDEEKARVFFNEVKGMREQYEVVEA"
     gene            complement(444..2414)
                     /locus_tag="IV250_002086"
     CDS             complement(444..2414)
                     /locus_tag="IV250_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769343.1"
                     /translation="MEIMGRSQLLNFKKKRKTKKEQKTRNSKVVNNTFVSDLTSLVGY
                     QFTPSYIRTGKRWGTIVKLVNTYGMNRNYGFGWMVKAIPEVRVEGVKAYLIEADKPVS
                     QKDQQEIMHENVGGSTNSYENNLEGKNSDAEISKTVMRIMDLNTASLLDSMANKIIDW
                     KLEILLVSDSPNKISEQLIKLQRLYDEKMYGLKLMSYGGDQRDLFDRLLSEPIGNVYE
                     YTSMSSDFAGNDHAIRKGLDDANGWAIGTMSTSYASGVAFMALDDSFTSKVLVASHKN
                     ATIKGYDEKISASSLWGQLIANNAMANHKRTFHIVLNKFDYFGDPTGRTFVAKPSLNS
                     VIEKVNLAEEGLNSLELFGNQKDIGDLYRRNLDKLTQIFYLLAGRTLDSATRVLLKKE
                     LGNFYISQKLWDINYEERSKLLVNSSDTMPVLGDFISYLTNAETKAKKSGTEKEVDRI
                     IVLKDTLRTALDSFSRMFNQRTTLKEIGKSDKLQFYYNLGDLRAETNVMEAQFLNTFD
                     FITSQAKKDDIVMIHGFDKLSIETVGLIKKRLTMLEEDGIRLAFLFDTIGSGEDNEYK
                     EDIETADIFNTDGKIYQSFESDFNYTIIGKMTSGELMKYEKKVAPARKGEDDQKLSVD
                     LRQYLTTGRSNKFQIRRPVDLTNNFVIGEFIL"
     gene            complement(2429..3295)
                     /locus_tag="IV250_002087"
     CDS             complement(2429..3295)
                     /locus_tag="IV250_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769344.1"
                     /translation="MADNSNYGFKHNLHQKPSETIVRVEWLEKHVNSTYSVTAKRLIV
                     SAIVVAVVVLGSIVYLVSDTIKLKFIFVPTLLMIIGFLILISQYMFIDPSSGSLISGS
                     IRLFKSVVNSARVSMGKKKPFRGTEILKVKQDGTIVYRDGSVALMFNLDGATSLTAFP
                     EEVMAQESKSIQYQNTRDRSLAEIKITSSQPQNTERQTRNMKIQKRNARSNKAVFDII
                     DQQERFVSEYIDGVKTTVIQYLILKSQSKMVLDEGVERLENYTSRGLYYSVTQLSAKQ
                     TKEILSDIYALK"
     gene            complement(3285..3518)
                     /locus_tag="IV250_002088"
     CDS             complement(3285..3518)
                     /locus_tag="IV250_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8769345.1"
                     /translation="MREEKIVYPVIQNNQENMTSSQHLQEAQLVYQVNELPQTNETMS
                     KSISLIGLLIVAIVITVGVYRKLNVKGSISDGR"
     gene            complement(3530..5686)
                     /locus_tag="IV250_002089"
     CDS             complement(3530..5686)
                     /locus_tag="IV250_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769346.1"
                     /translation="MAKFKDITESINYVAETVPVVALSLKPQLLEVYKKEEKEAKAKK
                     AKAKVWKEFDALLTKDLPKKNYMVIEDVQKLNSEGVIDEDDAILDSEKYVVLLKSSLN
                     YVADLPEKFKEKLLAGVRFMDSSGQLLVIPDDPNVFESEVEEATDFYSAVEQLEVATN
                     GLERIAETFNSSEEVEFEAVAENVPDEKEALFVDDNKPTEAENQEVSIEETQIQTETY
                     QQPKIDEKPESQSDKENYVYEAEKITSESDVPKNLDADFLEKNFEQIIADKFQPVVLD
                     EINDPVDLNEEMLSIPELKPLLFLAKESMNARISIANNKINSELNKTKEQLILKGKQM
                     LADELLRIEEATSLNGQNQFTNTLSVAKESYEAEASKIDDLVMEKSNIIRKDYDMAKQ
                     AYLDAELKKLEQRYDAEHLPEVKQQEDEYRKATEESLDQQYQEAIESIKNDALKQSQE
                     AKEVVVERIINSLKPEIEKSLTDVQEKTLEIVEKTKSENEAEFHDTKEKTTDVVVKYF
                     ANKEEVEKQVANNTKELETKLVEQQKTIEEYKDRMKEVKDLAEGYKLNSEKLAEQSEA
                     LREDLSRSRSDLAQMAEKTKSLAVATENQSSSKRDFTSKKMATIISVAAMACGTILGS
                     SYFISHNNQQAQAQTQTSISRVENSIGTNTNSASTNSSYEATPTTEFKVGKDVVISID
                     GKTTKAEVISIDGEKAVVKTPDGNQYKIQAHLSDNN"
     gene            complement(5706..5927)
                     /locus_tag="IV250_002090"
     CDS             complement(5706..5927)
                     /locus_tag="IV250_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769347.1"
                     /translation="MVFDREKMLAHANEVLMSSLKGTELAKIMNMNVNQFYDYRNGSK
                     KIEKARLETLIKFEKAYVYMLDKQKRTID"
     gene            complement(6037..6375)
                     /locus_tag="IV250_002091"
     CDS             complement(6037..6375)
                     /locus_tag="IV250_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769348.1"
                     /translation="MFDSIKNLVSGMTPDMKTLVQVVLYFLAFVFSLKPGFTAMKFFG
                     DAKWGDGLKYVGAIIAIFIIPVITTVMLYTMGTKTGNDLNQKANIISALIPVVGIYLV
                     SKFPKNELLG"
     gene            complement(6775..10416)
                     /locus_tag="IV250_002092"
     CDS             complement(6775..10416)
                     /locus_tag="IV250_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769349.1"
                     /translation="MKFKKYLLQNLRIKRWVVRGLVIGGVLAVTLGAPFGMNDPTPDN
                     QDSKQETVINKNVVYAESKPAEQGMWTLYARILMGDKEVNDSAAKTGQMNLLGQGGIS
                     ADFPYSDISKQGNSIAGGNAGTQLASTLATYSYYGYIETVSGNAIASQASSILGSIGR
                     TIGSFIGSISLILSNIAESINELLGKFFMSMNIFSLMGWGEGGSDVSNPVSKALTSLL
                     NNLGLNNELFKAIASLSMLFFTAILGYQGMKLLSKDGVNSKGLSPVLEKWIVRIFVLF
                     IFLPVAGSLLGVYINGIQSAKKDGDIPTNVVSDYIINTRLWASTQNLAPSSDTGGDVP
                     DGKPTSEIKFVDTDYEPSKKSAMIRAINTSSYARQNNDLDSNSVGMALLKEWSSNDTF
                     NINTYAGDVNVAENDAYMGRAPAVWTGYNLGDGKEGSAIKKPTNLNGYIWSANQNVTD
                     DNRKPDGKGTAHNFDATQPWGVANNATFSTQSVALLLQSALDTSGAKFYAYNIAPSGI
                     QGSAKNMSTVKTEWRTASLVGHGAMGKFGSWLAMNAESIVLAVLYLACAWAMFNVNIV
                     KAFKREFIRIWEVVLTGSLASGAGVMAISLGVVTTSLIGLQLPIFLITFIRAFGDTAV
                     GFIPNTLVGDGVANIVRSAMILGVGYLLVFKKGEKVNNLINMLIGLPLEVAFGFDERA
                     KLLFGNRNDAVRVVDGVSKAIKKSQGAKNPYYDGGNSSVKDMVAGGLGAVGAQKMAER
                     LNNMGDSNADAKSIADKVIPETPEGMSFNGGGVGSTINNTSFKDLVRQDQLRNNSQQY
                     IDHLGRMIDENGNFVDENGEILKDSKYGKDAKGNFIDEKGRLVDEDGNLIDTNGKPLE
                     DSEDGNIGMDSEGRLIDEKGRLVDEDGNLVDENGRRLLNGDFGQDSNGRLVDEKGRLV
                     DEDGNLVDEFGNKLGKHSDGYNVNGDLVDEDGNIIETRDALVADRNRELEQQRHTPIA
                     VAIPENEDDEITLDQTHQELRNEMSGEDVSDRVHRELGDGASGESQSRELNSNFDNSA
                     IRNDTALKDRESLTKETDKEQSDVKKKFIPTPNVNQTDKYETNIGSGMSKDNQSIREP
                     ELTNKNNRTNNSKSTSDNSGKSNQSTKKPSTAKSAVEYLSKTGKNVYTSADKALAKAP
                     KGSLAGITYEAKNEAIKIGKGTAKVAKATATGASKTGKVVKKSTMSVKDYIVKYGNQK
                     STENKSEDE"
     gene            complement(10419..12368)
                     /locus_tag="IV250_002093"
     CDS             complement(10419..12368)
                     /locus_tag="IV250_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAP8769350.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKSEDKENEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQL
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFK
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            complement(12410..13468)
                     /locus_tag="IV250_002094"
     CDS             complement(12410..13468)
                     /locus_tag="IV250_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HAP8769351.1"
                     /translation="MKKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLDERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVNKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            complement(13587..14042)
                     /locus_tag="IV250_002095"
     CDS             complement(13587..14042)
                     /locus_tag="IV250_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769352.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            complement(14625..15428)
                     /locus_tag="IV250_002096"
     CDS             complement(14625..15428)
                     /locus_tag="IV250_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP8769353.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKALSDKLGTLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
BASE COUNT         4590 a         3217 c         2237 g         5923 t
ORIGIN      
        1 ctgagatcac ctttcaatct aaatacacgg ttcttttctg gtaacttatt catgcttcaa
       61 ccacctcata ctgttctctc atacctttga cttcattaaa aaaaactctc gctttttctt
      121 catcagtaaa gtttttatac aactgattac aataatgagg tttttgaagt ttctgaatac
      181 ctatactaac ttcaaatcct tcttcatgtt gaattaggat tacttctgtg tctctaccta
      241 gaaatattcg atctaaaatt tttatgtttg gtttcattat ttaacctcca ctataattat
      301 ttcactcaat aaggtttcac tttatgaaat ttctatagtt ctcttttcac aaaacgtacc
      361 atgttctcta agactgtttt tagtctcaaa tatacatgtt ttttcttatc ctttccttat
      421 acactcaaaa tcaaagataa aacttaaaga ataaactctc caataacaaa attattagtc
      481 agatccactg gtctcctgat ctgaaattta ttacttcttc ctgttgtcaa atactgtctc
      541 aaatcaacag aaagtttttg atcatcttct ccttttctag caggagcaac ttttttttca
      601 tacttcatta actctccact ggtcatcttt ccaatgatag tgtaattaaa atcactttca
      661 aaactttggt aaatttttcc atctgtattg aatatatcag ctgtttcaat atcttctttg
      721 tattcattgt cttctcctga accgattgta tcaaatagaa aggcaagacg aattccatct
      781 tcttcaagca ttgttaatct ttttttaatt agcccaacgg tttcaataga taacttatcg
      841 aagccgtgaa tcataacaat atcatctttt tttgcttgtg atgtgatgaa atcaaaggta
      901 tttaaaaatt gagcttccat aacattggtt tctgctctta gatcacctaa gttatagtaa
      961 aactgtaatt tgtcactttt accgatttct ttcagtgtcg ttctttgatt aaacatccta
     1021 gagaagctgt ctaatgctgt tctcaatgtg tcttttaata caataatacg atcgacttct
     1081 ttttcagtac cagatttttt ggcttttgtt tcagcattag tcaaatagct aataaaatcc
     1141 ccaagaactg gcattgtatc actactgttc accaaaagtt ttgaacgttc ttcataatta
     1201 atatcccaaa gtttctgact tatataaaag ttacctaatt cttttttcaa taaaactcta
     1261 gttgcagaat ctaatgttct gcctgctaac aaataaaaaa tctgagttag tttatcgaga
     1321 ttccgtctat ataaatcacc aatgtccttt tgattaccaa aaagctccaa agagttaaga
     1381 ccttcttcag ccaaattaac cttttctatt acactgttta acgaaggttt ggcaacaaat
     1441 gttctaccag ttggatcacc aaagtaatca aatttattta agacaatatg aaaagttcgt
     1501 ttatgatttg ccattgcatt attagcaatc aattgccccc aaagtgatga tgctgagatt
     1561 ttttcatcat agcctttaat tgtagcgttt ttgtgtgatg ccactaaaac tttacttgta
     1621 aaagaatcat ccagtgccat aaaagctaca ccacttgcgt agctagtaga catagtgccg
     1681 atagcccatc cattcgcatc atctaaacct ttgcgaattg catgatcatt tcctgcaaaa
     1741 tccgaactca tagacgtata ctcatagaca ttcccgattg gttcacttaa aagtctatca
     1801 aacagatcac gttgatctcc tccatatgac ataagcttta atccatacat tttttcatca
     1861 tacaaccgtt ggagtttaat tagctgttcg ctgattttat taggagaatc ggatactaat
     1921 aggatttcta gcttccaatc aataatttta tttgccatgc tgtctagtaa agatgctgta
     1981 ttcaaatcca taatccgcat aacagttttg ctaatttcag catctgaatt ttttccttct
     2041 aaattatttt cataactatt cgttgatccc ccaacattct cgtgcattat ttcttgttga
     2101 tctttttgtg acactggctt atctgcttca attagatagg cttttacccc ttctactctc
     2161 acttcaggaa tagctttaac catccaacca aatccataat ttctattcat tccataagta
     2221 ttcactaatt tcacaattgt tccccaccgc ttacctgtcc taatgtaaga tggtgtaaat
     2281 tgataaccaa ctaaagatgt taaatcacta acaaaggtat tattcactac ctttgaattt
     2341 ctagtttttt gttctttttt agtttttctc ttttttttga aatttaatag ttgtgatcta
     2401 cccataattt ccatttaatc tctcacctct atttcagtgc ataaatatca gataatattt
     2461 cttttgtttg tttagctgac aactgagtga cactataata aagtcctcta ctcgtatagt
     2521 tttctaaacg ttccacacct tcatccaata ccatcttaga ttgagatttc aagataagat
     2581 attggattac tgttgttttc acaccatcaa tatattctga tacaaatcgt tcttgctgat
     2641 ctattatgtc aaagactgct ttgttactcc ttgcatttcg tttttgtatt ttcatatttc
     2701 gagtttgtct ttccgtattc tgaggttgag atgaagtgat tttgatttca gctaaggaac
     2761 gatctctggt attttgatat tgtattgatt tcgattcctg agccataact tcttctggaa
     2821 aagcggttag tgatgtagcc ccatctagat tgaacattaa tgctacactc ccatctcgat
     2881 atacgattgt tccatcttgt ttcactttta aaatctctgt gcctcgaaat ggtttctttt
     2941 tccccataga tactcttgct gaattaacta ctgatttgaa cagtctaata gaaccagaaa
     3001 tcaatgaacc actagatgga tcaataaaca tatactggct aattagaata agaaatccaa
     3061 ttatcattaa taacgttgga acaaaaataa atttcagttt aatcgtatca gaaactaagt
     3121 aaacaattga tccaagaaca acgactgcaa caactatagc tgatacaatt aatctttttg
     3181 ctgtaacaga ataggtgcta ttgacatgct tttcaagcca ttcaactctg acaattgttt
     3241 cagaaggttt ttgatgtaag ttatgtttaa atccatagtt tgagttatct gccatcagaa
     3301 atacttcctt tcacgttcaa cttacggtaa acacctacag ttatcactat tgcaacaata
     3361 agcaagccaa ttaaagatat tgacttgctc attgtctcgt tcgtttgtgg tagttcattt
     3421 acctgataca cgagctgtgc ttcctgtaag tgctgagaag aggtcatgtt ttcttgattg
     3481 ttttgaatta ctggataaac tattttttct tctcgcataa tagccctcct tagttattat
     3541 cagataagtg agcttgaatt ttgtactgat ttccgtctgg agttttcaca actgcttttt
     3601 ctccatcaat agaaatcact tcagccttag ttgttttccc atcaatactg atgacaacat
     3661 ctttcccaac tttgaactct gtagttggtg ttgcttcata gctactattt gttgaagcag
     3721 agttcgtatt tgtcccaatc gaattttcca ctctcgaaat agatgtttga gtttgagcct
     3781 gtgcttgttg attattatga ctaataaagt aggacgatcc caagatcgtt ccgcaagcca
     3841 ttgcagcgac agaaatgata gtagccatct ttttacttgt aaaatcacgt tttgaacttg
     3901 attgattttc tgtagcaact gctaatgatt tcgttttttc tgccatttga gctagatcac
     3961 ttcgggaacg agataagtct tctcttaaag cttcactttg ttctgccaac ttttcgctat
     4021 ttaatttata accctctgct aaatctttga cctctttcat tcgatcttta tattcttcga
     4081 tagttttttg ttgctctaca agttttgttt ctaattcttt cgtgttgtta gctacctgct
     4141 tttcaacttc ttctttgttc gcaaaatatt tgacaaccac atctgttgtt ttctcttttg
     4201 tatcatggaa ctcagcttca ttttctgact ttgttttttc gacaatctct aaagtttttt
     4261 cttgcacgtc tgttaatgat ttttcaattt ctggtttcaa tgaattgata attcgttcca
     4321 ccacaacttc ttttgcttct tggctttgtt tcaatgcatc gtttttaata gattcaattg
     4381 cttcttggta ctgttgatca agactttctt cagttgcttt tctatattca tcttcttgtt
     4441 gtttaacttc tggtaaatgc tcagcatcat accgttgctc tagtttcttt agttcagcat
     4501 ccaaataagc ctgtttcgcc atatcataat ctttacgaat gatattggat ttttccatca
     4561 ctaaatcatc aattttagat gcttctgctt cgtagctctc tttagcaacc gatagtgtat
     4621 ttgtaaactg attttgacca tttaatgagg ttgcttcctc tatccgcaaa agctcatcag
     4681 ctaacatttg ctttcctttg aggatcaatt gctccttcgt cttatttaat tcactgttga
     4741 ttttattatt agcaatagag atgcgagcgt tcatactctc tttagctaaa aacagcaatg
     4801 gtttcaactc aggtatggat agcatctcct cgtttaaatc gacagggtca ttaatctcat
     4861 ctaaaactac aggttgaaat ttgtcagcaa tgatctgttc aaaatttttt tctaaaaaat
     4921 cagcatctaa gtttttcggt acatcacttt cagatgtgat tttttcagct tcataaacat
     4981 aattttcttt gtcactttgg gattcgggtt tttcatcaat ttttggttgt tggtaggttt
     5041 ctgtttgtat ttgtgtttct tctatgctta cttcttggtt ttctgcttcg gttggtttgt
     5101 tatcatccac aaaaagagct tctttttcat ctggtacatt ttctgctacg gcttcaaatt
     5161 caacttcttc agaagagttg aaggtctcag caattcgttc taatccatta gtagctactt
     5221 ctaattgctc tactgcgcta tagaagtctg tagcttcttc aacttctgat tcaaacacat
     5281 taggatcatc cggaataact aataattgac cactactatc cataaaacgg acacctgcta
     5341 atagcttctc tttaaatttt tctggtagat cagctacata attcaaactt gacttcaata
     5401 gaacgacata cttctcagaa tcgagaatag catcatcctc atctataact ccctcactgt
     5461 tgagcttttg tacatcttca attaccatat aatttttctt tggtaaatct ttggtcagca
     5521 acgcatcgaa ttctttccat acctttgctt tagctttttt tgccttcgct tctttttctt
     5581 cttttttgta cacttcgagt aattgtggct tcaaagagag tgcaactaca ggaactgttt
     5641 ctgccacata attaatagat tcggtaatat ctttgaattt tgccattatt gtaaaactcc
     5701 ttttttcagt caatagttct tttctgttta tctagcatat atacatatgc tttttcgaat
     5761 ttaataagcg tttcaagtct ggctttttca atctttttac tcccatttcg ataatcatag
     5821 aactggttaa cattcatatt cattatcttt gccagttcgg tacctttcag actgctcatt
     5881 aatacttcgt tggcgtgagc taacattttt tctctatcaa aaaccatctt acatttactt
     5941 tcttccctca cttctaaact acgcttggag cgtagttttt tggtaaaaag taaattgaac
     6001 aaatcggcat attatccaaa tttgttcaat tcacttttaa cctaacagtt cattttttgg
     6061 aaattttgaa actagataga taccaacaac tggaataagt gctgatatga tattagcttt
     6121 ttggttcaaa tcattacctg ttttagtacc cattgtgtaa agcataacgg ttgtaattac
     6181 aggaataata aagattgcaa taatcgcccc aacatatttt aatccatctc cccattttgc
     6241 atcaccaaaa aatttcattg cagtgaatcc tggtttcaac gaaaaaacga atgccaagaa
     6301 atacaaaaca acttgtacta aagttttcat atcaggcgtc attccgctaa ccaaattttt
     6361 aatagaatcg aacatataaa aacctccttt ttaatatttt taataagtta attatatcat
     6421 cgacaaatct tatttgttca cttgtcaatg atctaactaa gaaaaagacg tctcgatatt
     6481 tacatcttta tgttatcata ataacataaa ttttcgatga ttaaaagaga ttttccatta
     6541 aattatttca ctttttttat tggctaaaaa tgctggttta tcaagttttt gtttagataa
     6601 taattctgtt ctttaccagc ataagccctt tttaattata caactttgaa cagtaaatat
     6661 ttaattcatt tatagacaat ttaaaataat ttttttgcat aaatagaaat tttccttgac
     6721 atcttcagtt agaaatcaaa gtaaaaagaa ggagcaaatc aatgctcctt ctttttactc
     6781 gtcttcagac ttattttcag ttgatttttg attaccgtat tttacaatat agtccttgac
     6841 tgacatggta gatttcttca ctactttacc agttttggat gctcctgtag cagtagcctt
     6901 ggcaacctta gccgttcctt tacctatttt aatagcttcg tttttagctt cataggttat
     6961 ccctgctaga ctaccttttg gagcttttgc taaagcttta tctgctgagg tgtaaacatt
     7021 tttaccagtt tttgatagat attcaacggc tgatttagct gttgatggtt tcttggttga
     7081 ttgattagat ttccctgaat tatctgaagt agatttagaa ttatttgttc tattattttt
     7141 atttgtcagt tcaggttctc taattgattg attgtctttc gacattcctg atccaatatt
     7201 tgtttcatat ttatctgttt gattaacatt aggagtagga atgaactttt tcttcacatc
     7261 tgattgttct ttatccgttt cttttgttag cgattctctg tctttaagtg ctgtgtcatt
     7321 ccgaatagca ctattatcaa agttgctgtt aagctctctt gactgacttt caccagatgc
     7381 tccatctcct aattcccgat gcactctgtc tgacacgtcc tcaccactca tttcattcct
     7441 cagttcttga tgagtttgat ctaaagtaat ctcgtcatct tcattctcag gaattgcaac
     7501 agcaattggc gtatgccgtt gttgttcgag ttctctattt cgatctgcca caagcgcatc
     7561 tctcgtctca atgatattcc catcctcatc gactaaatct ccattcacat tatatccatc
     7621 gctgtgttta ccaagtttat taccaaactc atcaacaaga tttccatctt catcaactaa
     7681 acgacctttt tcgtcaacta aacgtccgtt actatcctga ccaaaatcac catttagcaa
     7741 cctgcgtcca ttttcgtcaa caagatttcc atcttcatcg actaagcgac ctttttcatc
     7801 aattaaacgt ccttcgctat ccataccaat attaccatct tcactatctt ccaatggttt
     7861 accattagta tctataaggt ttccatcttc atcgaccaaa cgcccttttt cgtcaataaa
     7921 gttgccttta gcatctttac cgtatttact atcttttaat atttcaccat tttcatcgac
     7981 aaagtttccg ttttcatcaa tcatacgtcc taaatgatca atatattgtt gggagttatt
     8041 acgtagttga tcttgacgaa ctaaatcttt gaaagaggta ttattgattg ttgaaccaac
     8101 tccgccaccg ttgaatgaca ttccttctgg tgtttcagga attactttat cagcaatgga
     8161 cttagcatca gcattgctat ctcccatatt gttcaaacgt tcagccattt tttgcgctcc
     8221 aactgctcca agacctcctg caaccatatc ttttactgag ctgttgccac catcataata
     8281 gggattttta gctccttggc ttttcttgat tgctttagaa acgccgtcca caactcgcac
     8341 agcatcattt ctattaccaa ataataactt agcacgttca tcaaacccaa acgctacttc
     8401 aagtggtaag ccaataagca tattaatcaa attatttact ttttcgcctt ttttaaagac
     8461 taaaagatat cctacaccta aaatcatagc agatcgaaca atatttgcta cgccatcgcc
     8521 aactaatgta ttaggaatga atcccacagc agtatcacca aatgcacgaa taaaagtaat
     8581 cagaaagata ggtaattgta gtccgatcag agatgttgta acaacaccta atgaaattgc
     8641 catcacacct gctcctgatg caagtgatcc tgttagaact acttcccaaa tccgaataaa
     8701 ttcacgttta aatgctttca cgatattcac attgaacatc gcccaagcac atgctaagta
     8761 aagaactgct aaaacgattg attcagcgtt catcgctagc cacgagccaa acttacccat
     8821 cgctccatga ccaactaagg atgctgtacg ccattctgtt tttactgttg acatattctt
     8881 agcactgcct tgaattcctg aaggagcgat attatacgca taaaacttag ctcctgatgt
     8941 atcaagtgct gattggagca ataaagctac cgactgtgtg ctaaaagttg cattgttagc
     9001 aacgccccaa ggttgagtag catcaaagtt atgcgctgtt ccttttccat caggcttgcg
     9061 attgtcgtct gtcacatttt gatttgctga ccaaatatat ccatttaaat tggttggttt
     9121 cttaattgca cttccttctt ttccatcccc taaattatag cctgtccaaa cagcaggcgc
     9181 tcttcccata taagcatcat tttcggcgac attaacatca ccggcataag tattaatatt
     9241 gaaagtatca ttcgatgacc attcttttaa tagagccata ccgactgaat tagaatctag
     9301 atcattattt tgtctggcat aactgctagt attgatcgct cttatcattg cagatttttt
     9361 agatggttca taatctgtat caacgaactt gatttcggat gtaggctttc cgtcaggtac
     9421 atcaccacca gtatcagatg atggagcaag attttgagta gaagcccaaa gtcttgtgtt
     9481 aatgatgtag tcagaaacaa catttgttgg tatatctcca tctttttttg ctgactggat
     9541 gccgttgata taaactccca atagcgatcc tgccactggt aagaatataa acaatacaaa
     9601 aatacgaaca atccattttt caagtactgg tgataatccc ttcgagttaa caccatcttt
     9661 cgacaataat ttcattcctt gataacctag tattgctgtg aaaaagagca tagataagct
     9721 tgctatcgct ttaaataatt cattatttaa tcccaaattg ttaagcaagc tagttaatgc
     9781 tttagatacc ggattagata catccgatcc accttctccc caccccataa gagaaaatat
     9841 attcatactc atgaaaaatt ttccaaggag ttcatttatg gattcagcaa tattagaaag
     9901 gattaaacta attgatccga taaaactacc aatcgtccta ccaatacttc ctagaatgga
     9961 tgatgcttga ctggcaatag catttcctga gactgtttca atatatccgt agtaactata
    10021 agttgctaaa gtagatgcta gttgagtacc tgcattaccc cctgcaatcg aattaccttg
    10081 tttagaaatg tcagaataag ggaaatcggc actgatacca ccttgtccta acaaattcat
    10141 ttgtccagtt ttagcggcac tatcattaac ttctttatct cccattagga tacgagcata
    10201 tagtgtccac attccttgtt ctgccggttt actttccgca tatacaacgt tcttatttat
    10261 aactgtttct tgtttgctat cttgattgtc aggagttgga tcattcattc caaaaggagc
    10321 gcctaaagta actgctaata ctcctccaat aactagacct cttacgaccc atcgttttat
    10381 tctaaggttt tgtagtaaat atttcttgaa tttcatagct acgcccctcc aatcgaaaat
    10441 acaatcttgt tttctttact ttcagcaacg gaaggctttg caataatttc agcattagaa
    10501 tcaccttttc cttcaaacgc tccggcttca ctcaacttaa catcgaatat ttttgcgttt
    10561 ggataagtaa ttattgcttc agtaatcgct tcttcaaact cagattcaac taaatgcaag
    10621 atattccttg caccccctga tttagaatcc tcttgtgaag ccatgtcaaa aactaagtgc
    10681 atggagagat cgtctgcata aaaattttga ccatatactc tttctttcct aggtggaaga
    10741 cgtaaatcta tatttaaatg ttttcgtact aattcagcac tcttcaacat ttcattataa
    10801 gcaatttcca gaagagtttg ttcggataaa gcccaaaatg gaactattcg tccaaatcgg
    10861 ttaataactt caggctttag accttcacca atcaaggctt gtttgatccc tgatgcgact
    10921 tttcggtatt ctctttttag ttcagtgcta tattcattag aattttcctt gtttgtaaca
    10981 cgagatagtg agctatatct gtttaacgaa tcgaacatct ccgcacctgc atttgttgtc
    11041 gcaaataaaa ttgtatttga caatgaaacg gtgtcgtgaa cttctcgatc atcccatcct
    11101 ctttcagtaa aacttatcct accttcatct aaaattgata aaaaggcatc gagtgtttct
    11161 acaccagctt tctcatattc atcacacaca ataatcgcag aaggatcatg ccttacatga
    11221 gtacctatga gtcgtctaaa acgcattgca tcatcttttg ttttgtagtc cggcatatta
    11281 actaagagaa ctttattttc atcgccataa agtcctcttg ccacggcttt agccattgca
    11341 gttttaccga cccctgttga tcctgtaaac agagcagttg cttttggttg ctttcgacct
    11401 ttaaaacgat caaatccaag cttcatacgt ttaaacaaca ttgttatagt ataaactgct
    11461 aacggttgcc cttttatgga ctttaattcc tcttgaagtt tttccatatc aaaatcagaa
    11521 tctagctgaa tattatactt accaaatatt tgcaatagac tttctcgatt ataagcattt
    11581 ccgtctactc gatcttttgc aatcaaatcc tccattacgg caattgattt agccggttcg
    11641 gacaaatcct cacgataaag cttgtttgct tgtacgattt cttgaagtaa ttcatcagga
    11701 atcctttgcg ataatttaaa agaagcctga ggatcatctt taacagagga tgtatttagc
    11761 cattgtctca aaatatcgac gacaacatca ctttttagtt cattcaattc taacggttgt
    11821 aaccgtctcc ctaaggcatc atctcttttg atatagtttt catattcttt tcttgtagta
    11881 gcggtaatca attgaagtga ttcatttgct aacggttctt ttagcgaatc acccccaatt
    11941 ttattgccac taccaaaaga gctaacaact ttatgaaact cgtcaatgaa gaggacaaat
    12001 tcattttctt tgtcctcaga ttttaaaatt cgctcatatt ctttcaatct tggaattaat
    12061 gtttctaaac gtgtttggag gacatcatct cccccaacac gcaattgtcc gattgataat
    12121 ttgtatactt gtactttctt tccatcttgt cgctgtcttt cagcccattc acttgcaagt
    12181 gctgttttac ctgttccggc ttcacctaac aagatagctt gcggtgtttc cggtctattc
    12241 aagatcagtg acaactcatg gagttcgtca tcacgtccca ctaacttatg tttcggtggt
    12301 ttcattggtg tgagagcatg attaaattca gtttgaatat tcttaaaaaa ctcggtacta
    12361 ttttccatct gtttgtgttc acttcctcta aaataagaat aatttacttc tagtcagtca
    12421 gagttaggta cttaaatgtc gtcttcatgt ctccaaaaga atctttgtta aagtcagcat
    12481 ttttgtattg atcaatcaat ttttgtgtat cattgattgc tttaatttcg gtctccattc
    12541 gactgtctag ttgacgaata ctttcttgcg aatctttatc gtcaggattt tgttgtaaac
    12601 gttccagata ctcagctttt gtagcttcaa ttttcttgat gttagtattt gaagcattta
    12661 actgatcttc tagcttattt acaacattat tcttacttgt aactgctaca acttttgaat
    12721 aatcaatatc gccatctttg gtaagaaagc ttccttgata gacattagtt gaatctgaat
    12781 atggatttat tctgaaattg atattatcag gagtattaac aggttttgct tttttcccaa
    12841 aagaaattac tccttcatta ttaacatcat tcatagaata ttgagaaagt gcttttgtaa
    12901 ctgaatctgc ttcattcaca ttgactttgg tatcatcatc agctgtatca tcaccagaaa
    12961 ttagtgatac ttggtttgca ataaaaactt gataagctct ttcatctaat ttacctttta
    13021 ggaataagaa agcatcccca tcagtactta acattccata gctcatttca agcttcttag
    13081 gacgatcttt ttcttttgtt gccatatata agttgtagtt ttttccttgc gtagataaca
    13141 actttctagc gttttgcgag taaccgagtt ttactactgt tacatctctg ttttgatctg
    13201 tccacacatc tgaaatagtg atctctgctc ctgatcgagc aaactccaat gaagagccta
    13261 taggtgttgt attgttcacg agataataat tatagtttcc taattgataa atgtagctac
    13321 ccaagcctgt taataaaaaa agcactccta gaaaagttgc aagcataatg cgtaatccgt
    13381 gacgtgttct cttatcctta tcaccaagca accctccaat aatactgtat tgatctttgg
    13441 tagatgttga attgacgttt tttttcatta gtttgtccgc cccctttcat aaaagagcaa
    13501 acaatcagac gttttttcgg taattctagg ttcgggattt ttaatcattt cggtatattc
    13561 ttccccatta atatttaaca caaaaattat cttcctttct tcgttccttt cttttgctct
    13621 ttattttcca aataatcaat gatttttgct attgtaccaa gatagtaatt cccctcggct
    13681 ttgttttttc ggattttgct aaccattgga gtaggtacaa tctcttcatc tctcaattca
    13741 ctgaaagatt ttttacctag atattttcca ataacagatt ttaaaaaacg ttcaaaatct
    13801 ttctcgtaag ttgttttctt gccttttagt tcatggttca tattgtcaac gacttttgcg
    13861 gtttcaataa ttgttttagt tctcagctta ataatttcat ctggaatttt caatcctcgt
    13921 aatttcatcg ttgtactaac tggtaaccca tgctttactg catcagcaat actaaaactt
    13981 tttagatcat ctagtaagct ctccatatag ttaacaaatt catgccaatt aatagcattc
    14041 attgtctcac cccttcattt ttttaaaatg aggttcgggc gtaacacatt tatgttatta
    14101 taatatcata ataacataaa taaatcaatc agcaaattta gatatccctt aaagtatcag
    14161 caattattca acaagatatt tattttagct atttcagtca tcatgaaaga ctaattagca
    14221 ataaaataac taatattatc catttatcag acgaaaagaa aaaacttatc taattactct
    14281 atattaactt ttaacaaata gttgacttgt ccaaaaatgt taccttcaat ctcacaaaga
    14341 tattaagatg tttcctacca aaactgatat caccggactt tttattctgt gttgtccaaa
    14401 agtgttacct ttagtcgatc aacaaattaa aaaaattgtt tttttcttga cttgtccaaa
    14461 agtgttaccc atagttgtta aaatttgtat agattagcca ttctttcaaa tattatttag
    14521 actgaaactt gcttacaaaa aaacttcttg catttattgt ccaaaagtgt tacccatact
    14581 tttttaaaga agcaggttgt attaaaatta caacctgctt cttctcaatc ttcttctaac
    14641 caattatgca ttgaaatggt tggcaagttt gattctggct cactaaaagt gaatctgagc
    14701 cgatcaactc ttcttccttt ctttgcatag attttttcat aatcaaaagt ttcaaatacc
    14761 tgtattccgt tttcatcagg agctaaaaat tcttctttcg cctgctctag tattcttttg
    14821 tcaatatttg tcattctata acttgaaggt atacttaatg ctcttctaaa atcttcaact
    14881 ttgactaccc aataaccatt tcgccactct ttatttctgt attgcatgag atgacgatac
    14941 aattcttttg tgtagctact tcttagtttt gtaaaattat caagttcaaa acgagtaaaa
    15001 tttttgccaa ttgagtttaa gataaattca aattcctcat ttgccttaat tgtaactgtt
    15061 tccttctgtt tatctatccg gaatttttga aaaagccaca gctgttcata gacgtctgaa
    15121 tcttcaaaac ggtaggttaa agtacctagt ttgtcactca aagcttttaa tacagaataa
    15181 aaactgtcct tttctcttct gtcgtatttg accaaataac gaaagaggtt gaaatcaaaa
    15241 gttacttctg ctgttccttg acgtttcatt tttgataaaa tagcccaaaa cacatccatt
    15301 tcaacaggct taaatcctcg caaagctgtt gtattcagac cattgttata acgcacaatt
    15361 gcatttgttt gttctgatac taaatctaca tcactatcaa taatcttttc ggtaacttct
    15421 tgagacattg attactctcc aatcttcttt ttgcgctcct ttaatcatgt taatctggta
    15481 ctaattaaag gaaacatttt tttctgcttg ttacctaaat taaatcacaa ctaaaggtaa
    15541 cagtcaacaa agatttgtta ctaatctaat tttctatttt ttcgccttcc aaatcaagaa
    15601 aaccgaaggt aacgtcttat cctagtatga tacctcaaat gtccactttt gttacctttg
    15661 ctgtccaaaa gtgttaccct tactgtccac ttttgttacc tttactgtcc aaaagtgtta
    15721 cctttatacc cctttaatac ttactctccc aagagattaa gcctccccta acagatatat
    15781 taacagatat ttaacagata tattaacaga tatttaataa aggaggatta ctcctctctt
    15841 atttcaaaat cttaaaaaaa aactaacact atctaggagt ttattttgct tgtgtaactt
    15901 tgaataattg aaaaaaagga aaaggaggaa gcttcagctt ccccttttat ttatatttat
    15961 aataccc
//
LOCUS       DACDIE010000055        15262 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.055, whole genome shotgun sequence.
ACCESSION   DACDIE010000055 DACDIE010000000
VERSION     DACDIE010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15262
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.055"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(21..251)
                     /locus_tag="IV250_002097"
     CDS             complement(21..251)
                     /locus_tag="IV250_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769354.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            complement(687..1253)
                     /locus_tag="IV250_002098"
     CDS             complement(687..1253)
                     /locus_tag="IV250_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP8769355.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1253..2209)
                     /locus_tag="IV250_002099"
     CDS             complement(1253..2209)
                     /locus_tag="IV250_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAP8769356.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2209..2865)
                     /locus_tag="IV250_002100"
     CDS             complement(2209..2865)
                     /locus_tag="IV250_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAP8769357.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3235..5253
                     /locus_tag="IV250_002101"
     CDS             3235..5253
                     /locus_tag="IV250_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8769358.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     gene            5680..8094
                     /locus_tag="IV250_002102"
     CDS             5680..8094
                     /locus_tag="IV250_002102"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAP8769359.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8442..9995
                     /locus_tag="IV250_002103"
     CDS             8442..9995
                     /locus_tag="IV250_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP8769360.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10376..12160)
                     /locus_tag="IV250_002104"
     CDS             complement(10376..12160)
                     /locus_tag="IV250_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8769361.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(12175..13896)
                     /locus_tag="IV250_002105"
     CDS             complement(12175..13896)
                     /locus_tag="IV250_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8769362.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13996..14757
                     /locus_tag="IV250_002106"
     CDS             13996..14757
                     /locus_tag="IV250_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP8769363.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         4967 a         2793 c         2998 g         4504 t
ORIGIN      
        1 ctcattaaaa agcataacca ttactccgat ctgtttttaa aattagtttc tacctctaat
       61 atatatcgag tttttatttt gccttcagag aatactgttt ctttttttgc agttacaggt
      121 tgtttatttt cggaaaaagc taatatagct aagattgaaa catccatctg gaatttatct
      181 tttttactgc tttgatcata aaagtctgca tattcatcac tgatattttt tctaataaat
      241 tcttccatca taaaaatcac ctcaaaacga ttatacgaac ttacgttctt gttgtaaagc
      301 gatatttgag gggcaaaaaa ggggcataat ttgactgata ttaagttagc tattgatata
      361 tgaatgacat acaaaaatat aaaatctttt aaaaatgctt atatatcaat gtttttgcca
      421 ttcttttata tatctgttat atgtcaaaaa aatgtgttca atttcaattt gtatttgaat
      481 aaagaggttg tgaaaaagct gtcctgcatc aagtaataag aacagccaat caaaaacagc
      541 ttctcatgtt tttgatttgg ttggacttat taccgcagat ataagctttt gagcaccgtg
      601 aataaagagg ttatgaaatc aacgctctaa cctgagtatt agagaaaaaa acgcaaaaca
      661 tatcatttat gttttgcgtt ttttatttat ctttttttct caatacaata taaaataggc
      721 ggctggttct tttggttgat aaaccgataa ttcagcacat tatatgtttc ctgtggcagt
      781 ttggaacaaa aagaatcaac catatccagt tctttttctc caccttcatg accatagtag
      841 acgacaagga tcaatcgtcc gcgaggcaca agtcgtttca atatttcttc catagctgtg
      901 cgtgtagtct ctggcatagt gatgattgct ttatcacttt ttgggagata ccctagattg
      961 aaaatccctg ctttgagatt ttgttgttca gctatcactt ctcctaacgt ttcgtggcct
     1021 tgtagaaaca atgagacacg ttcttctaac tgacgctgtt ctagcctttc acgagtagca
     1081 tcgattgctt gttgctggat atcaaaacta tagacatgac cagtttttcc gatgtgttct
     1141 gctagaaaaa ccgtatcgtg gccattgccc atcgttgcat ctacgacgtg atcccctggt
     1201 tgcaagattt cttgcagtaa tgtatggctg aaatgcaaag cagtttgcaa catcagacgc
     1261 tcaccttttt ccgtacatca tattttcctt ggaagctatc tctgcgaaga agttcttcat
     1321 caattgcatt caagacttcc cattttttca agctccacat tggtccaatc aaggaatccc
     1381 acggcgcatc ccctgtcaaa cgatggatga tgatctcttt tggtatcatt tccagctggt
     1441 cgcaaatgac attgacatag tcaggacggc tcattaattg aaggcgacct tcatgatagt
     1501 cccgcaacat tctcgttttg cgcatcagat gcatcaaatg gagcttgatt ccttggatat
     1561 ctgaatctaa aatggtgcgt ctgacatttt ctttcatcat ttctaaactt tcgcctggta
     1621 atccgttgat caaatgcgta caaacacgga tattatgttt gcgtaattta gccacgccat
     1681 ctaaataagt ttgataatca tgtgctcggt tgatcagttt tgaagtttct tcaaaagttg
     1741 tttgcaatcc aagttctacc cataaataca tccgctcgtt cagttcagca aggtaatcaa
     1801 ctacttcatc tggcagacaa tccggtcgtg taccgactga taatcctacg acacctggta
     1861 aattgactac ttgctcaaat cgttctttga ttatttcaac tggcgcatgg gtgtttgtaa
     1921 aattttggaa ataaacgatg tattgttgga catgaggcca tttttgatgc atcatgtcga
     1981 tttctttctg gaattggatt ggaagagggt cttcaggtgc aacgatcatg tctcccgaac
     2041 cagatacact gcaaaaagta caaccgccat gggcaacagt cccatctcga ttcggacagt
     2101 caaatcctcc atcgatcggc accttgaaaa ttttcccgcc aaactcattt cgtaaagcgt
     2161 aattccaaga atgatatctt ttatttcctt ctgaataagg aaaagtcatt aaaagtcctt
     2221 ctttctgtaa gagttatcta tttgttttct tttttctcta tacactggat aagtcaggca
     2281 cagccaagaa agactgagta agtagccgcc gatgatatca gtaggaaaat gaacgcccag
     2341 ataaatgcgg cttatcccga cacttactat aataaacatc atcagcaatt gaagcaatgt
     2401 gcgtaaggtt cttttttgaa caaaagctgg aagcagaaat agtaaagaac cgtaaagaat
     2461 catactgcct gctgcatgtc cactagggaa actgtaactt gtttcaacta ctaagtgcgc
     2521 caatgaagga cgctctcgca tgaaaaatag tttgatcacc gggttgatga ttccagcaat
     2581 tacaataaat ccagaaacga accaaaatgt ttctgccctt tttttcttat accacatcca
     2641 taaagccgca caagcaaata aaatgatgac ggtaagagga ttaccgaatt tagtcataaa
     2701 gagttggtag ctgttcagat caggataaga agctctgaca atgcctgtaa accactgatc
     2761 gaaaccagaa agccacgaca gatggaactt tacgatccag cccaaaatga aaaacaggac
     2821 aagaaagaag ctaccagcca cctgaaatga tgttttatat ttcatacaat tcctcctttg
     2881 aagcaatgac ttattatagc atttttggtc cagcgaaaaa aacggtaaag agataaaacc
     2941 aatgtatgat tcctctttga ataaccaata ttcttataaa aaaaataact gtaatcggaa
     3001 agacattttt tggtaatgca aatgaaatgt tagggaaata aaatcagtgt ttttcgaaat
     3061 ccttttaaac aagaacgtag acgtaggaag aacttgaaaa tgaacagaag ttgttcatta
     3121 cggtaaatta caatatgatt acaaaaaaca acaaagctct taaataactt ttaataaata
     3181 ttaagagaag ttataattat taataataga taaattgaag ttggaggata gaagatggaa
     3241 aacattgcaa gaaaagaacg gagacgttta gaacagacca aacgttttcg aaaagcaaaa
     3301 cgaggggcag caatcgtggg aactgcgatg gtaggctgtt ctgtcgctgc cccattagta
     3361 cagccggttc ctgttcaagc ggatgaaaca cctgttcagt tcggcgcaag aatcaataca
     3421 gcggcattta tcacagaaat tgctacttat gctcaaccaa tcgctcaagc caacgatcta
     3481 tatgcatcag tgatgatcgc acaagcagtt gtagaaagcg gttggggcag tagtgcgtta
     3541 tctcaagcac cttattataa tctttttggg ataaaaggca gctatcaagg acaaaccgtc
     3601 tacatggata cgcttgaatt tttaaataat caatgggtaa ataaaaagga gcctttccga
     3661 caatatcctt cttttgctga atctttcaat gacaatgcct atgtacttag aaatacttca
     3721 tttggcaacg gttattacta tgccggtact tggaagagca acacgaaatc ctatacagat
     3781 gcgacagctt gtttgacagg gcgttatgcg actgatcctg gttacgcagg caaattaaat
     3841 aatatcatcg caacttacgg attgacccaa tatgatacac cagctagcgg aaacgcaggc
     3901 ggtggagcga ctacaggtgg caataatact ggaagtgcag gtagtactgg cggttcaagc
     3961 aattctggta atacaaactc aggttcagca acttcgacca cttatactgt ccaatctggt
     4021 gattctgttt ggggaatctc aaataaattc ggtatcacaa tggatcaatt gatccaatgg
     4081 aacaacatcc aaaacaattt catttatcca ggacaaaaac tgacaatcaa aggtagccag
     4141 gaaaatggct cttcgacgaa taattccggt aacaatacca actcttcagg aaatgctggt
     4201 acatcaaacg gaggacaaac aactggtgcg aaatacactg ttcaatctgg tgattctgtt
     4261 tggaagatct caaatgatca cggaattacg atggctcaat tgattgaatg gaacaatatc
     4321 aagaacaatt tcgtttatcc aggacaacag ctaatcgtat caaatggcgg ttcttctaat
     4381 gctggagcaa atactggctc aaatactggg aatacaagtg gctctagttc gtccagctca
     4441 agttcttcta atacagcttc aggaacgaaa tatacagtta aagccggaga ctctgtttgg
     4501 agcgtctcaa ataaatacgg aattacgatg gctcagctaa tccaatggaa caatatccaa
     4561 aataacttta tctacccagg acaacaactg actgtttcaa acagtggatc aacaaacagc
     4621 acgtcaggta atacaggttc gtcaaccaat acaaataaca gcagttcagt taatactggc
     4681 agcagttcgt ctcaaggcac ttataccgtg aaagcaggtg aatctgtttg gagcgtggca
     4741 aataaaaacg gcatcacgat ggatcagtta atcgaatgga ataatatcaa aaacaacttc
     4801 atttatccag gacagcaatt gatcgttaaa ggtggaactt cggcaaatac aaatacaggt
     4861 tcaactactt ctgcagcgaa accaaataca ccgaatacgt ctgctacgac aagtacatca
     4921 tccactggta atacgatgta taccgtgaaa gcgggcgagt cagtttggag cgtagcgaac
     4981 aaacatcata tcacgatgga tcagttaatc gaatggaata atatcaaaaa taatttcatc
     5041 tatccaggac agcaagtgat tgtgaagaaa ggttcatctc aatcaacaaa ccaacaatca
     5101 gttactggtt cgaaaaccta tactgtgaaa tctggagaat ctgtttggag tgttgctgac
     5161 agtcatggaa ttacaatggc tcaactaatc gaatggaaca atattaagaa caactttatt
     5221 tatccaggtc aaacgttgat tgtagctaaa taaaatagct gttcgaagaa ataacgattg
     5281 aattccacaa aaatatttgt tactataagg ctacttaaat atgacaacac ttgaaaagga
     5341 agtagtaaaa agactgaagt ttcagagagc gcatggctgg tgagaatgtg cacggatgct
     5401 tttgaactcg cctttgagtt gcccttgctg aatacccgag taagcaagcg gcggtgacgg
     5461 ccgttacaac gttcgaggcc ttttcactaa ggtaaaatta ggtggtaccg cgtagattac
     5521 gtcctataaa aagagcaatc tttttatagg atttttttat ttcttttgtg aagtttgaga
     5581 aaacaaagca aaaggaaata aaaataaaca gattctgccg gttttttcgt tatatttata
     5641 atagaaacaa gtgttttcaa tagaacagga ggacagcaaa tgagttacaa tcataaagaa
     5701 attgagaaaa aatggcaaaa atactgggca aaaacgaata ctttcaacac ccatgatgat
     5761 cctgaaaagc caaaatttta cgcactggat atgtttccct atccatccgg tcaagggcta
     5821 catgtagggc atcctgaagg ttatacggca acggatatcc tttcacgtgt gaaaagaagt
     5881 caaggataca acgtgctgca tccaatgggg tgggatgcat tcggtctgcc tgctgaacaa
     5941 tatgcgctag atacaggaaa cgatccagct gagtttaccc aaaaaaatat cgaaacattc
     6001 cgacgccaaa tcaattcttt aggatttagt tatgactgga atcgcgaagt gaatacaact
     6061 gatccggagt attataaatg gacacaatgg atctttacga agctttatga aaaaggtcta
     6121 gcttatgaag cagaagtagc ggtaaactgg gtacctgaat taggaacagt tatttcaaat
     6181 gaagaagtga tcgatgggaa aagtgaacgt ggtggctatg atgtagtcag aaaaccaatg
     6241 cgccaatgga tgctgaaaat cactgcttat gctgatcgct tattggatga tctagaatta
     6301 gttgattggc cagaaagcat taaagaaatg caaagaaatt ggatcggtcg ttcagtagga
     6361 gcaaatattg aatttaaagt ggctggtacg gataaatcct ataccgtatt tacgacacga
     6421 ccagatactc tattcggtgc aacttactcg gttcttgcac ctgaactaga tcttgttaga
     6481 gaaattacga cccctgaaca aaaagctgct gtagaagctt atatcgaaga gacagctaaa
     6541 aaatcagatc taaagagaac ggatcttgca aaagaaaaaa caggagtatt tactggtgct
     6601 tatgcaatca atccagttaa tggcaaggaa atcccaatct ggatcgccga ttatgtatta
     6661 tcttcatacg gcacaggagc catcatggct gttccagcac atgatgaacg tgactttgaa
     6721 ttcgctcaaa ctttcggact agaaattctt ccagttatcg aaggtggaga tgttgaaaaa
     6781 gctgcttaca cagaagatgg tacgcacatc aactctgaat ttctagacgg catgaacaaa
     6841 caagaagcaa tcgataaaat gaatgaatgg ctagaagaaa atggcgtagg gaaaaaagaa
     6901 gtcagctatc gcttacgtga ctggctattc tcccgtcaac gttactgggg tgaaccaatc
     6961 ccaatcatcc attgggagga cggaacagtc acagctgtcc cagaagaaga gctaccatta
     7021 cggttgccta agacaaaaaa catcaagcca agcggtactg gagaatctcc attagcaaat
     7081 atcgaggagt gggtcaatgt cgttgatcct gtaacaggta aaaaaggccg tcgcgaaacg
     7141 aatacaatgc cacaatgggc aggaagctca tggtattacc tgcgttatat cgaccctcat
     7201 aacaaaaaag aactagcaga ttatgaaaag ttgaagcgtt ggctaccagt tgacatctac
     7261 attggtggag cagaacatgc ggtacttcat ttactatatg cacgattctg gcataaattc
     7321 ttatacgata tcggtgttgt tcctacaaaa gaaccattcc aaaaactata caatcaagga
     7381 atgatcttag gcgaaaacaa cgaaaaaatg tcaaaatctc gtggtaatgt agtcaatcct
     7441 gatgatgtgg tcgaaactta cggtgcagat acactgcgca tgtacgaaat gttcatgggg
     7501 ccattagatg cttcgattgc ttggagcgaa aatggcttag aaggaagccg taaattcttg
     7561 gatcgtgtat ggcgtttgat cgttgatgag aacaataaaa tgcgtgaccg tatcactaca
     7621 ttgaatgatg gcaagcttga taaagtgtat catcaaacag tgaaaaaagt gacagaagac
     7681 tatgaaaatc ttcatttcaa tacagcaatc tcccaattga tggtctttat caatgaagcc
     7741 tataaagtgg acgctctgcc ttatgaatat atcgaaggat ttgttcaact actcgcaccg
     7801 attgctcctc acatgggtga agaactatgg gcaatccttg gaaatgatgg tggtatttct
     7861 tacgcaccat ggccaacata cgatgaagca gcacttgttg aagacgaagt agaagttgtt
     7921 ttccaagtaa acggtaaagt tcgtgcgaaa agcaatgttc cccgagactt aggaaaagat
     7981 gagctagaaa aagcagcatt agccaatgaa atagtgcaag aatatattga aggcaaaaca
     8041 gtcagaaaag ttattgcggt accaaacaag ttagtcaata tcgttgctaa ttaattggct
     8101 ctttgtcaat aaggactgat gagttgtgca aaatcaaatc tgggccagaa tgaacttcat
     8161 tctgtctcag attttttgtt atttcaaatt gattaattga ttgatcaaaa aggttttgat
     8221 tttcatgttc tcaaacacgt tcaatgtctt tttcttctga atggtcttgt aagagatctc
     8281 tgtaatcatc aaatttttgt ctattaatct tagtattttt tgatgtataa actacctgaa
     8341 attgagagta cataccaaat tcataaattt tttattagtg ggactagtga ttgtatggat
     8401 tttgacgcat aatgaatgaa gtatgagaaa aggagagaca aatggatcaa gaactattta
     8461 ttatcgaagt attatcagct aaacaaaaaa ttatcgagaa agcgaaaaaa agagcgaaaa
     8521 gaataatgtt caagctttta agcttagtat ttttctctgg tttgcttttt ttcggagctg
     8581 tatcgttcct atatcgccat atgagtgcag tagtccgttc agctgaaggg atgcaaaaat
     8641 taaataattt gtatcacttt tcgtggggag ttggcggaat aacaattgtt gtgcttattg
     8701 tttttcttct acttatttgg ctccgttacc atcaattgtc gcgtcatttc gatcaatggc
     8761 ttagtcaaag agcacaacag tttttacata ataaaccaac ttctcaagat gatgactatc
     8821 tttattttga gaagacttcg acgagtcaag cttttcggat acaaaaaagt gattgccagt
     8881 tattgctgag atcacttgat gatttaccta tctacttagg cgaaagcaaa tcaaaattcg
     8941 gaatgaacac attccatatt tttttactag aaaacgaacc cagatgggaa aaaagccagt
     9001 cttcgcctgg tggatacctg acaactaaga tgagtacatt agtcactggt tttttcctca
     9061 ttcttttagc tggaacaatc tattatggga gatacactca gccaaaaatg tcttctcata
     9121 taaatgctgc ttctcacatg acaatgaact caactaacga tacgcatata caaaagacaa
     9181 atgaagggtt gttagctgat cctaagcaaa aggtaaattt gacaacacaa aagataaatg
     9241 atctcaaact tgatacagag acgcactctc tctatatgac aacagatagt ggtgctaatt
     9301 gggtatttgt gccgattgat tcgcaatggt tgcgttttgg cgaatacacc ctgacaacgg
     9361 ggctgattcc tgaaggttat tggatggaca acacatatga tatttccgcc gatttctcat
     9421 ggtttatcta ttcttctggg gatgatgtac gtctgctgac ttcaagagat aatggtaaaa
     9481 catggcaaac gtctattatc acccaatcag ttcagccgat acgttatcga aaagcggtat
     9541 tttatggcaa cgatggtcta tttatctgta catcagccag tgggatgtct gcagaaactt
     9601 tgtacattta cactactatg gatggcggaa tcacttggat aaaaagtgga agtacaatga
     9661 tcaatcagcc agtacaaaat gtcagcttcg ttacaacttc tttaggtttt gtttcgacac
     9721 gtgaaagttt gttttataca aacaacggag gacgctcatt caaagaatca gttctgtcga
     9781 ttccggaaaa ctatcagaca ggggggatag atctttttca atcaccaaat gaagttactc
     9841 aaataagcgc gaatacgtta gagacaaaat tttatttgtt gaaaaaagga ccaatcgatg
     9901 cagggaaaat gtttgcctgc ctcttccgtt caactgataa cggggaaaca tggcaattcg
     9961 ttcagcaact atcgcaagta gaacaaggag attaaagaag agagattgtg agcctgacgt
    10021 tcagcaccga gtcacaagaa acaaattcaa aaaacagctt ctcatatttg ttgaatagtg
    10081 taactcgtga ttcggagttg acaagagcaa caagaagcga ttttgaagta tagcttctcg
    10141 tattttacaa gatcgtgtct tattgccgat aggttgtctc ttgaacaccg tttattcgta
    10201 aaaagggatt gtgacaagtc ttttgtcact acttaccgaa cgaaactgaa ggagctatcc
    10261 aaaaagtatc attagtatta ttctttgtgt ttctagtggc accactagta catttaaaaa
    10321 agctatcgat catggactaa tcatacatga tcgatagctt tttagataat gtttgttacg
    10381 ctatctgaaa ttgactatta taaagactgg cataaagcgt tggtgattgg agtagttcag
    10441 aatgtgttcc tttttcgacg atatcgccat tcttcattac aaggatcatg tctgcatttt
    10501 caatcgttga caaacggtgt gcaatcacaa aacttgtcct atcttttgtc acagcttcca
    10561 ttgcttgctg aatcaatgct tctgttcgtg tatctacgct agaagttgct tcatctaaaa
    10621 taacgacagg aggattcgct agaatgactc gagcaattgt caataattgt tgctgtcctt
    10681 gagataacgt gccgttttcg ctagaaatga tcgtatcata tccgtctggc agtgttcgaa
    10741 taaagtgatc acattgtgcg atttcagccg cttcgataat ttgttctctt gtggcatcca
    10801 ttttcccgta ggcgatatta tcagctaccg ttccttcaaa cagccaggta ttttgtaaaa
    10861 ccatcccaaa agaacgacgt agttctccac gtgaaagctt ggtaatatca agaccgtcga
    10921 attcgatctt tcctttattc agttcataaa aacgcatcag cagattgact aaagtcgttt
    10981 ttcctgcacc agttggacca acgatcgcta ccgtttgttt tggctttact gaaaaatcga
    11041 cttgattcat caatatttta tctgatgtgt aaccaaactg tacatttttg aaagctattt
    11101 ttcctttcgg ttgttctaaa agtcttgcat ctggaaggtc tggaatttca tctgtttcat
    11161 ctaatatctc aaatatccta tcgattgctg ccattgcgga ttggatcgag ttgatcacat
    11221 aagaagcggt agaaattggt tcagaaatct gattgatgta ctgaagataa gcttgtaaga
    11281 agccgatcgt cacaccacct gatagaacaa gcatagcacc gaataccgca cttatgatga
    11341 aggcgagttg attaatgaaa cgaatggccg gatagatagc acagttcata aattgagcac
    11401 gtttaaaagc gttgtattgc tttttattta cttcgtgtat agcagcttct gcatgagctt
    11461 gctgattgaa tgttttgacc actagattac cagctaagat ttcttccatt cgattattta
    11521 aaacacccag ttcatactga ttctgttcag aaatctgttt gtttttgttc gcaatcttag
    11581 ttgtaacaat tgaactgcca acgatcagca ataagacgag caaagtaagc ttgctttcga
    11641 tataaaacag catcagacca gcagcagcta ttgttacgac agaagtaaaa aattgattga
    11701 tacctgtcaa taacacttga gaaagttgat tgagaccggt agttgtccga ctaatgatgt
    11761 cacctacttg atgattgtca aaaaacgaca tcggtaatgt tttgaatttt tttgtgattt
    11821 cttttcgcac cctcaatgtg atcttttcgc ttaacgctgc cattgtccgt tcctgaataa
    11881 atgaagtgat gctgcttaag actgaacagc cgatcagcaa taagacaggg aacaacaatg
    11941 cttgtttgat atcactgaaa gaaagctgtc caatacctgt ttttcttatt tctgataaga
    12001 gatcatcgat cccgattccc atgatcaagg gcatggctac gactaatagg ttcccgaata
    12061 aactgcatag aaacaatccg taaaaatatt tatgttcagg tttgatcatt ttccaaaaac
    12121 gtatcaaagc acgcgtgttc aacgtttttt tcttttccat cgtatccctt ctttctatat
    12181 tccttgtgat tttgcaaact cctgataagt ttggcaatta gacagtagtt cttcatgagt
    12241 accttgtcca acgatttttc cttcatctaa aacaatgatg ttatctgctt gttggattgt
    12301 actgaggcgt tgagcaacaa tcacgagagt tttctccgac atgtgtttat gaagggctgc
    12361 tcttaatgct gcatctgttt tatagtctaa agcggagaaa ctatcatcga atacatagat
    12421 ctctgctggt ttgatcaacg ctcgagcaat acatatccgc tgtttctggc cccctgaata
    12481 attagaaccg ccttgagcaa caaagctgtc caatccgtca ggaagggtgt ccactagttc
    12541 ttctaattga gcgatcttga tcgctttaga caactcttct gttgaggcat attcatttcc
    12601 catctgcaag ttgcttaaaa ttgtcccgct gaataagaaa gccttctgag ggacataact
    12661 gatgtgggag cggatctgtt cttgagtcat ttgagtaaga ggaataccac caagggttac
    12721 ttttcctatc gtagggtcac taagtctcaa gagaagtttc gccaaactac tttttccaga
    12781 tccggtcccg ccaacgatcg ctgttgtttt tcctttaggt attgtgaaat caatatcttc
    12841 tagaactgga tcagagattt cagtatattt aaatgtgaca tgttcaaaag cagctgcatt
    12901 tgtctgatca aaggttggtt cagctactgt tttgatcgtc tcttcctcaa tttcgatctc
    12961 tgcttgcagt atttcttcta tccgtcgtaa tgaggcaaac gatttaggga taatgaccaa
    13021 taccatagct gcgatcatca agtaagcaag tgtcagcata gagtattcga ctacagcagt
    13081 cacgacaccg atttgtaatg ctccttctgc tacaagattt ccgccaaacc acaaaataga
    13141 agcataaacg attcccatga gcaaaaaagc aattggagtg acccaagcaa atattcggtt
    13201 cactttgatc atttgattag catagtcagc aaagctctta ttcgttcgct tttcttcata
    13261 tgattgatta tcaaaggcac ggatcatatt aatcccagta aagaattctc tcaatgtgat
    13321 ggtgatccga tccatttttg gctggatact caaagataaa ggtgcccctc gtttcatcag
    13381 tcgataaaca atccatacat aaaaaacaac agagatcaat gtaacccatg ttagtaatag
    13441 agaataacga aacatcatga gaatggtgaa aacgcaaata accggtgctg gtaagatcat
    13501 ttggcaaaat aaaacaatca tttgctggac attatcgaca tcattcgtca ttcttgtcaa
    13561 aagagatcca gaactaaaac gactgatatc cttcattgac agctgttgga atttacggaa
    13621 atatttctcc cgcgtttgga atccaaacct agcggaagcc aatgctgaaa agtaactaga
    13681 ggcaactgca gcgattgttc ctagaaacgc tacaagcagc atttcaactc caattttttt
    13741 tataactgag gcttcgccac ttgctatacc tgtgtcgatc agctttgcta ctaattttgg
    13801 tacacctaat gtaccggcga tttctaaaca gatcatcaca aaggtaagga gagcgagtcc
    13861 tttgtttttc tgtaagaatg tttttaagat agacatatta ttcctccttt tttcctgata
    13921 cttttcgatt ctataccttc aagtaacttg aaggtcaata gaaaaaagaa taaactgaaa
    13981 aagaggtgag aaaaaatgaa aaaaatgtat acgattggcc aagtatcgga actatttgat
    14041 ttgccaaaat caacgatccg ctattgggat gaacaaggat taatccgttc aagtagacaa
    14101 gaagagaatg attatcgatt gtttgatatc gatgatattt ttatgcttta tgatatcgtt
    14161 ttttatagaa aattagatat accgatcaaa cagatgaaga atttatatgg aaaaacattg
    14221 acggagttat atgagacact tgatgaaaca gaaagaagaa tccataaaga actggtggtg
    14281 atgaaacaaa aacaaaaaga gataagggag caaaaaaaac agttgaaatt gatgatcgat
    14341 acaaacgagg aagaatttcc tgtagaagag atcccttttg actgtatgat ctcaacggag
    14401 tttgaagata ttgtggaaat caaaaaattt ctacctaact attcaagctt tgggatgatg
    14461 agtatgccag cttccagttc acagacgatg tatggatttt ttatcgaccc tagtgaagta
    14521 catttattta ctcaagacgt gatttgggag aaaaaagata cggctgttta tcgtcgattc
    14581 ttattgaaat ccgagatgaa ccatgcggaa cgtaataata ttttagaaat cagagaaaga
    14641 atgtatgaaa aaggatggaa aacaggagaa gtgatcggcc agtatctatt gacgaatacg
    14701 gatgaaaaca atatcagaac tgaatattat catgcatgga tagaaatgaa aaaataggaa
    14761 atatgtgttt aagataactc gaaaatgatg gttgttttag ttataaagaa aaattttctg
    14821 gttcgattac gggtttaatg tgggggatta ttagtcgtgg atagatagaa ttttagtttt
    14881 ttagaggaaa tatgtcttta aaaatggaat ggttgcaatt cttagcgata tatacgatgt
    14941 aattgtcctc ttaaaaaagt atggaaaaat aattgtattg ggtgttatgt ataattcatg
    15001 gtgaactgag actaatgaga agaaaaattc atattgttat gagtgagatt gtatcagatg
    15061 agaatgataa attaaatagg tgcataaaag cattacactt aaaatacatt gttgcttaca
    15121 ttattacaat atgttaataa ggcaaaaata aatagatgga ttgaataaac atatttttta
    15181 atcaaatagt ccctactgtg actttcctca gctatcggtg atactaccga tagcattttt
    15241 ttgcttataa aataattata ag
//
LOCUS       DACDIE010000056        15217 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.056, whole genome shotgun sequence.
ACCESSION   DACDIE010000056 DACDIE010000000
VERSION     DACDIE010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15217
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.056"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(24..722)
                     /locus_tag="IV250_002107"
     CDS             complement(24..722)
                     /locus_tag="IV250_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobyric acid synthase"
                     /protein_id="HAP8769364.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(715..2067)
                     /locus_tag="IV250_002108"
     CDS             complement(715..2067)
                     /locus_tag="IV250_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HAP8769365.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2290..3690)
                     /locus_tag="IV250_002109"
     CDS             complement(2290..3690)
                     /locus_tag="IV250_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HAP8769366.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3851..4588
                     /locus_tag="IV250_002110"
     CDS             3851..4588
                     /locus_tag="IV250_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAP8769367.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4713..5192
                     /locus_tag="IV250_002111"
     CDS             4713..5192
                     /locus_tag="IV250_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HAP8769368.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5189..5956
                     /locus_tag="IV250_002112"
     CDS             5189..5956
                     /locus_tag="IV250_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769369.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6186..6755
                     /gene="lepB"
                     /locus_tag="IV250_002113"
     CDS             6186..6755
                     /gene="lepB"
                     /locus_tag="IV250_002113"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP8769370.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6859..7461)
                     /locus_tag="IV250_002114"
     CDS             complement(6859..7461)
                     /locus_tag="IV250_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP8769371.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7567..8036
                     /locus_tag="IV250_002115"
                     /pseudo
     CDS             7567..8036
                     /locus_tag="IV250_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8142..8726
                     /locus_tag="IV250_002116"
     CDS             8142..8726
                     /locus_tag="IV250_002116"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAP8769372.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8729..9802
                     /gene="prfA"
                     /locus_tag="IV250_002117"
     CDS             8729..9802
                     /gene="prfA"
                     /locus_tag="IV250_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAP8769373.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9795..10634
                     /gene="prmC"
                     /locus_tag="IV250_002118"
     CDS             9795..10634
                     /gene="prmC"
                     /locus_tag="IV250_002118"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAP8769374.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10627..10806
                     /locus_tag="IV250_002119"
     CDS             10627..10806
                     /locus_tag="IV250_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769375.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10828..11853
                     /locus_tag="IV250_002120"
     CDS             10828..11853
                     /locus_tag="IV250_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAP8769376.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12028..13272
                     /locus_tag="IV250_002121"
     CDS             12028..13272
                     /locus_tag="IV250_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAP8769377.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13425..14372
                     /locus_tag="IV250_002122"
     CDS             13425..14372
                     /locus_tag="IV250_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8769378.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14506..15135
                     /gene="upp"
                     /locus_tag="IV250_002123"
     CDS             14506..15135
                     /gene="upp"
                     /locus_tag="IV250_002123"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAP8769379.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
BASE COUNT         5188 a         2720 c         3111 g         4198 t
ORIGIN      
        1 tcacattccc ctttttcgaa taactaggct gtttgtcctt caagataggg cagatccaat
       61 tctttaccgt aacgttgttc taaagctagg cggatcaaac gagctgccag gttctcattt
      121 cttgcaagga taggtccatg gaaataagaa ccgaagacat ttcggtaaat gacaccttca
      181 gattggtctt caccattatt tccttgacct tgtaccactt tccccaaagg tcgttcacct
      241 tcacctaaga aagtccgacc attgtggttt tcaaatccat aataagtttc attgaattct
      301 tcgttatgga taacgatatc accgataaac cggttatttt cctgacttaa cgtgtagtga
      361 tccagtgcac tgattccttg gatcttttct ccattagctc ccatgtagta gtgacctagc
      421 aattggtagc caccacaaat ggctaacatg acgccatcat tttcgatata gttgatcaaa
      481 gaatctttct ttttctggat gtcttttgaa atgattaatt gctcaaagtc ttgaccaccg
      541 ccaaagaaaa ccaagtcata tttatcagca tcaaaaggtt catgtatact aacgatctcc
      601 gtatggcaag aaacccccat tttttctgct atatatttca acatcaaaag gtttccgtta
      661 tcaccatatg tgttcatcaa atttccataa aggtgggcga tattgagttc ataattagcc
      721 acggttcatt cctcctttga taaatccttg agcagctagt tctttacgaa gctgcaatac
      781 agcagtgtaa gtagctaaaa tatagacatg ctcagttggc aattctttga ttttttcgat
      841 tacttctggt aattcttttg tttctgtcaa tttattttct ggaatcccgg caacttttag
      901 acgtaaagtc atatctgtat gacggtctcc tccagaaata acagctggga tatccatatc
      961 ggcaaagttt tcaaaattac catcccaaat ccagctgaca tcgattccgt ctgcgtaatt
     1021 ggcatttaat aaagccacta aagaaaatgg ataaggagct agaccaatca tatcgattac
     1081 ttgattgata cctacaggat ttttgacaag tactaatgta cattttttcc cattgatatt
     1141 gatgacttct tgtcggccga atactttctc gtcatatcct aaaccttgtt tgattttatc
     1201 aggatcgaca ttgaagtact ctgccactgc agtagctgct aaagcattgt agacattata
     1261 cataccgcca acttcgattc cgtattcatg accatcgatc acgaaatctg ctgacacatt
     1321 gtccatccgt accatatcag ttaatcgata atccaattca ggacgtttga agtcacaatt
     1381 tggacagtaa tatttcccta agtttgcata agtgatcatt ttataatgca agatgtgatg
     1441 acaatttgga cataagacac cgtctgtatt gtaatgagcc atttgttcac gatcttcttc
     1501 atggtcaaag ccataatatt ttctaggatt gactgtttcg atagaattga agattggtga
     1561 atcaccgtta ctgataatcg ttgcatttgg tgactttgcg gcaccgtcta caatcatttt
     1621 ataggtcgta tagatttctc cataccgatc catttgatca cggaaaatat ttgtaaataa
     1681 gaacagttct ggtttcatat attctgtgac tttgctcaaa cttgcttcat ctatttctag
     1741 gacagcaaac tttttctgtc ctttttttgg ttttgcctgc aagaaagtcg atacaattcc
     1801 ctgtaccata ttcgcaccag tagggttggt tagaacttca tcgaattgct ggcgcaaaat
     1861 attgactgtt aaagcggtag tcaatgtttt accgtttgtt ccggtgacaa cgacgacttg
     1921 gtagtctttt gccaaactat ctagtatatg tggatcgact ttcagcgcta gtttacctgg
     1981 taaacttgat ccgcctttaa aaaaagtttt taatgcccat tgcgacgttt ttccaactgc
     2041 aatcgcaaaa tgactgcgta ttcccattat gtaacctcca aaaacgattt gctgacgaat
     2101 aaattcaact aatcatacca tatgttgaaa aaaaggttaa attgcttttc ttattcttaa
     2161 gaaaaggtta cggctgaaag tgcatgaaat cggacaaaat cgcatgattt aacaaaaaaa
     2221 cagcttgaaa caaaatgatt gtttcaagct gctgtgattt tattattccc tcttacctat
     2281 attcccattt taaccgataa taattttctc gcttggatat tcatagccta gatctcgtgt
     2341 ttcttttggc acgaataaca tcaatgtata tagcattccg atccgcccaa taaacatcag
     2401 tgtagcaatc gtgattttgc ccacaacaga caagtctccg gtaatcccta aagataaacc
     2461 agttgttcca aacgcagaag ttacttcgat gatgatcgag atcagcgttt gttcttctgt
     2521 agcagataaa aaaacgatac aaaagaaaca cataccaaga gaaagcataa agacaacgac
     2581 tgattttcgc acatcatctt gatcgattct ccgtccaaaa atattgatgt tatcttcact
     2641 tttgaggaag gaatatagat acaaaccgat gattgctaca gtagttgttc gaatcccgcc
     2701 gcctacagaa ctaggactgc atccgataaa catcagtaaa gaaaaaatga tcagtgttgt
     2761 gatttgaaaa tcacccaaat cgtgaatctg cagaccagca ttacgagtcg taatcgaata
     2821 aaacatcgaa ttgatccatt tgacactcat attggaatct tggaataaat ggtccttttc
     2881 caatagataa atcaagacag ttcccgaaac aaacaagata acaaaagcta acacagctaa
     2941 tttggtaaac aaggaaaaac gaaacggaag cttcgcattg gaacgtttgt ataatagcca
     3001 ttcccggcac tccatcaata caggaaaacc aattcctccg acaaaaatca aaaacatgat
     3061 caggatcaag aaaaaatagt catgtgcaaa gggcttgatg gaatccccag tgacgtcaaa
     3121 cccagagttc gtgacagccg atatcgcctg ataaaaaccg taaaaaacgg catcacgcca
     3181 acgatcaaaa tagcctctgt aataaaaata aatagaaaag aatgtcccaa aaagtaattg
     3241 aaaccagatt aaaatagcaa aagtgatccg aatcagtcgg acgataccag acaaacgcgg
     3301 ttgattcata tccgtcatga tcagctgtcg ctgtttcagt gtgatccgtc gtttagaaaa
     3361 aatgacaaaa gcagtagaaa tcatcatgat ccccaaacca ccaacttgaa ataatatttc
     3421 cagcagtata ataccgttat cattgaacac agaattgata tcaaaagtcg atagtccagt
     3481 cacactcact gtactgattg ccatgaacag catgtccaca aaagaaacat ggctatctgg
     3541 ttcgcgaaaa atcggcatat aaaatagaac gagtgataag accgtcatca atatataata
     3601 aaaaacgatg atttggatcg atgagaaatg attggcagca aacttctgtc ctcgccgctg
     3661 cagccttcga aagaataaat caacatgcac agtactatcc tcctgacaat agtcgtttct
     3721 accagtatac aaggggaatt aggaatactc aaagaaatta tcaaacagat aacaatacag
     3781 ctttcgatta aaatagaaat ggcttataat aaggaaaatc ctagaaaaaa gaaggaggag
     3841 gaataacaaa ttgattaatg ctcaacaaat cgtagaaaaa atgaaaaatc aaaaaataaa
     3901 ttatgataca gttctgaaaa aaatgatcat gcaatgggag aaagaagaag taaagcctaa
     3961 gatattaata catacatgct gtgctccttg cagtacgcac acattggaat tcatgtctca
     4021 atatgcagag atcacgttat ttttttcaaa ttcgaatatt catcctaaaa gtgagtattt
     4081 gcgaagatta catgaacaaa aacgatttgt agaccaattt aatgagaaaa ctgaccatca
     4141 tgttgcgcta atcgttgatg aataccgtcc aaatgaattc accaaaatgg ttttaacaaa
     4201 ccatcttgaa aacgaaaaag aaggtggtgc tcgctgcagt gcatgcttca atatgcgatt
     4261 agatcttgtt gcgcaaaaag cgcaagaact aggatacgat tactttggaa gtgccctgac
     4321 catctcgcca aagaaaaatg cagctttgat caatcagatc ggaatggata tccaaaagat
     4381 ttacgggaca caatacttgc caagtgattt caagaaaaac aaaggctatg aacgctcttt
     4441 gcaaatgtgc aaagagtatg atatttatcg ccaatgttat tgtggttgcg tgttcgctgc
     4501 caagcagcaa ggatgcgatc tgaaagaaat caatcgggaa gcaaaagctt acttaaaaac
     4561 atcaaccgta gtttttgagg aaaattaaag aaatctttcg gaaaaacgta agaaaaaaat
     4621 aaagaattta tggtagaaat aaggtagact gtataatttg tccgtagtaa agtaaggaaa
     4681 caagaataaa tttcaatgaa ggaagagata agatgcgaaa agtagcacct attttgaagc
     4741 caatcgctgt attaacaatc tcgacgattg tcgcgactac tacaggtatt gtgaatagtt
     4801 ccagctaccg tgcaccaagt tcaagttcta gtactgtaga agaaacaaca ccaactactt
     4861 cagtctcgac atctgaacca acaacaacgg aaagtttgaa tgaaaccact acgacaagta
     4921 catgggacga tacaacaact agttcgtcgt atgatgagac atatacaact gacagctcaa
     4981 gcgaaacaag taatacggta ccagatggca ctccatcgga ttcaaccaat ccaagtacaa
     5041 gtccggatac aagtgctagc tccgaaacga taccaagcag tacgactgta gatgataaca
     5101 cgcaaaatga tgatgcagca cgtacagcac agcaaaacca agattcagga aatacccaaa
     5161 cgtataacca gcaagcaggt gattctcaat gacattttta ctagacctta tcaacggggt
     5221 gcataaattc cttggctatt tagacatcag tcctaaatat cttaaccgtg gatatacgat
     5281 tttaagtgtg attcccacgc tttatttatt gagaattgtt tatggacttt ggcaaaacca
     5341 aaactatctt caatttttct tatatggact tgcatttcta gtgcttgttt attttactgt
     5401 tttaaatgta ttttattact ttttagataa aaacacgaaa gccgatgtca ctcagctttt
     5461 tgtgaagtat ttacctgatg aagcgttcaa catccagaca gaagaatcga tagataatgg
     5521 aaattcgatc gataaagtga atacacaaga ggtcatggtt tcttatgacg aagattatca
     5581 attgaaatta gccgagaata tgcggtattt gattggaaat ggtgagatca aaacaaatga
     5641 actcggtcgc atggatggat ttctggtcga tagaaataca ttgtatcctt attactatgt
     5701 caagaaaaca ggagagaaag agtatgttct aaatattgga cgaagctact cggacatgga
     5761 aaaagtaggg accattcgac ttgactcaac agaagacaca ttggaacctg tcggattgtt
     5821 catcgtcggc ggtgattttg tcaaagacgg gttacgctat catgagcctt atcgtctgaa
     5881 attgattgca aaaaaggcac agtcgcagga agaaccagtg gtttatagtc gtagccaaag
     5941 aagaagacat tcataatgtg atgaaacgcc gtgtatcagt ggttgcgata aaaacgttta
     6001 ttcggaataa gggggatgtg acacgtcttt tgtcacgttc cttttttata taacgagaaa
     6061 gataagacga acacatcact tattctttcc tttcatttat aaacattgta gaataaagaa
     6121 agtacaacga agtattgtga aaaaacgtaa taaataacga ggaaaataca agtaggaggt
     6181 aagctgtgaa agaatttttg aaaaattggg ggattttgat ccttgtatta gcggctattt
     6241 tattagctag agtgtacgtg tttacaccag ttactgtaaa cgggcattcg atggacccta
     6301 cattaagtga tggacaacga ttgatttcat ctaagatctc aaactatgaa cgaatggata
     6361 ttattactac aaaggaaccg ggggatgaag aacggatgat tgttaaacgg atcatcggta
     6421 tgcctggaga tacagtcaag atggaaaatg accagttgac cattaatggc aaaaagtatg
     6481 atgaacctta tctggatgag ttcaaaaaag aattttcaga agataagtta caaggagaat
     6541 atgcttatag ctcaggattc caagcacaag cagaaagttc atccaccttt acgagtgact
     6601 ttgaatatac tgtgccaaaa ggaaagtatc ttgttctagg cgataaccgc ttgatttcaa
     6661 aagacagccg aatgtttggg ttagtcgaca aagatatgat ccaaggaaaa gttgttttcc
     6721 gttattggcc tctttcagaa attaaaatca tgtgatgaaa aaataaaaag aagctgtgac
     6781 atgttttttg tcacagcttc tttttccgaa aattccttct tatttcttgg agttgctcac
     6841 tttttttgca atctcatttt aagctttttg gctgatggat aagaaaagcg aagcgttttt
     6901 gttcataaat agcttttccc cacgatggaa ggtatgacta gtagcagaga caaagcccgt
     6961 tttttccaat cgattattca attcttcttg gctaaagcca ttatggactt tcggatgact
     7021 aatttgttca ttcttgtcaa agtctacgat gatcaattga ccgccaggag ctaaaatttc
     7081 ataaagtttt gtaagaatat tctccgtatc aggaatgtgt aaaagtacaa gtgatagaag
     7141 gatgagatta gcttggacag caggaaattc gacagaagca tcagcgtgga tcgttcggac
     7201 atttttcaga tctgctgctt ggatcttttc ttctgccatt ttcagcatag tttctgaagc
     7261 atcggcaatg atcagttctt tgaatctttc cgcaagcggt aaactaacaa gcccagttcc
     7321 gcctccgtaa tccagcagga ctttttggtg gctgtcgcgc ggtagataag tagttagttc
     7381 ttcgcgaata atcttggcta atgcttgacg gtcaggggaa tcataatggt tagcgatgga
     7441 atcaaaaata ttttttgtca tcaaatagct ccttacgtaa aactttgaaa ttttaactta
     7501 ttatagcacg tgaaaagaaa gaaagcatgc tatactcaat tcaaaaaggg agaatctaga
     7561 aagaatatgc cgatatcgtt tgtaaaagat agagaggaaa aaggaaagtg tgtccgagaa
     7621 atattactag acttgcctga gtggtttggg cttcctgaat caacggaaaa atatatcgag
     7681 gaatcaagta aactaccttt atggtgtgaa aaaagaaaag aagaatattt aggctttatc
     7741 actctttcac agacaagtga agatactgcc gaaatctatt ctattgtatg ggaataaaaa
     7801 aagactatca tcaccaagga ttaggaacaa aactttttaa agaagcagaa ggttacgcag
     7861 caaaacatta caaatatttg caagtgaaga ctgtagacga ggggcactac tctatatatg
     7921 atcaaactat ttgtttctac gaatcattag gattttcaag gttggaagtt tttccaaatt
     7981 tatgggatga atggaaccca tgtttagttc ttgtcaaaaa attggaacaa aagtgagcaa
     8041 tagatttaat actaatctgt gcttgatgaa aattctcact gtaaaaaatg ctgaaaatca
     8101 agtataatac cataggaaca aactggaagg tgaaaaagag catggcacaa ctatttttta
     8161 agtatggcgc aatgaatagc ggcaagacga tcgagatcct aaaagtcgca cataattatg
     8221 aagaacaaaa taagccagtt gtcttaatga caagcggtat tgatacaaga gatggtttag
     8281 gcgttgtttc aagccgaatc ggactgaaac gtaatgcgat gccgatattt gaagaaacag
     8341 acgtatttga cgtaattcag aaaatggaaa gcaaacccta ttgtgtgctg atcgatgaag
     8401 cgcaattctt aaaaaagaaa catgtcattg cttttacgag aattgtggac gaattggata
     8461 ttccggttat ggcatttggt ttgaaaaatg attttcgaaa cgagttattt gaaggatcaa
     8521 aatatttatt gctttatgca gacaaaatcg aagaaatgaa aacaatctgt tggttctgcc
     8581 acaaaaaagc aatcatgaat ttacattata ttgatggaaa ccctgtttat gaaggcgatc
     8641 aagtgcaaat tggcggaaat gaagcttatt accccgtttg tcgaaaacat tactttcacc
     8701 catcaatgat tacagaagga gaataagcat gtacgatcaa ttacaagcaa ttgaagaccg
     8761 ttatgaagag cttggagaac ttttaagtga cccagaagtg atttctgata caaagcgttt
     8821 tatgcagctt tcaaaagaag aagcaaacac gcgcgaaaca gtcgaagttt atcgtcgtta
     8881 taaagaagta gtacaaggaa tcaccgatac agaagaactg cttggtgaaa aactggatga
     8941 tgaaatggct gaactagcaa aagaagaatt atctgaatta aaaaaagaaa aagaagtctt
     9001 agaagaaaaa atcaagatct tgcttttacc aaaagatcca aatgacgata aaaatatcat
     9061 catggaaatc cgtggagcag caggcggaga cgaagcagca ttattcgctg gcgatctttt
     9121 caatatgtac caaaagtatg ctgaagcaca aggctggaaa acagaagtga tggaagctaa
     9181 cgttaccgga atcggtggat ataaagaagt gatcatgatg atcaccggag acaacgtata
     9241 ttcgaaatta aaatatgaaa gtggtgcgca ccgcgtccag cgtgttcctt caactgaatc
     9301 gcaagggcgt atccatactt ccaccgcaac agttgtcgtg atgccagaag ctgaagaagt
     9361 agagatcgat attgaagaca aagatatccg tacagatatc tatcatgctt caggtgctgg
     9421 cggacagcac gtcaataaaa cagcatctgc tgtacgtttg actcacttac caacagggat
     9481 cgttgttgct atgcaagatg aacgttctca aatcaagaac cgtgaaaaag caatgaaaat
     9541 cttgcgtgct cgtgtgtacg ataagatcca gcaagaagca caaagcgagt atgatgccaa
     9601 tcgaaaatca gcagtcggaa ctggggatcg ttcagaacgg atccggacgt ataatttccc
     9661 gcaaaatcgt gtgactgatc atcgtatcgg attaactatc caaaaattag atcagatttt
     9721 agcagggaaa ttagatgaga tcgttgatgc attgatcatg tatgatcaaa catcaaaact
     9781 ggaagctatg caaaatgggt aaaatgacct atcgagaagt cctgtcacgg gcttcttctt
     9841 ttttagagga tcatgggaaa gaagcatata gtatccaatt tttatttttg gaacgaaaac
     9901 aatggaaaaa acttgattgg ctccttcata tgaacgaaga gatttcagaa gaagaacagc
     9961 ggttgatcga aaccgatctg cgacttctgc ttgcagatca tccacctcag tatttgctag
    10021 gatatgcaga tttttatgat catcggttaa aggtaactga agcaacactg atccctcgac
    10081 cagaaacaga ggagttagtc gaatggtgtt tagacgagac gcctggtgta ccgttagaag
    10141 tgatagatat tgggacaggg actggagcaa ttgctattag tttgaaagca gcaagaaaaa
    10201 attggcatat atctgctgtg gatctttcag aagaagcatt agaagtagcg aaagaaaatg
    10261 ctcagaaaga aggaacgaaa atcagttttt atcatggaga tacattagga ccagtaatgg
    10321 atcagcaatt tgatgtgatt atttctaatc cgccgtacat cagtcggaat gaatgggaac
    10381 tgatggatga aagtgtccgg tcattcgaac ctaaaatggc cctttttgct gagaacgatg
    10441 gattagctat ttatgaaaaa atagcagttg aagcaccaag cgtactaaaa tcaaatggaa
    10501 agattttttt agaaatcgga tttcgccaag gtgaagcagt aaaaaaaata tttcagcaag
    10561 catttccaga taaaaaagtg gcgataaaaa aggatctttt tggcaatgaa cgaatgatcc
    10621 aagtttatgg ttgaagttgt ggatgaaaaa ttttttattt gtttttttat tataaaaaaa
    10681 cacttatttg acaaggatgt aaacgacttt ttggtttatc aacaagttta tccactattg
    10741 acaaatagat tatccacaaa cagtggaaaa cttgaggata aactcagaca aaataagaat
    10801 gtatgaaaaa ggggttgtgg ataaactgtg gaaacgaaac gatatgatga aacagaattg
    10861 caagaggcag ccgaagcact gaaagaagga gaacttgttg cttttcctac cgaaacagta
    10921 tacggattgg gcgccaacgc tcttttacca aacgctgtca agaaagtgtt ttctgtaaaa
    10981 gggagaccgc aagataatcc attaatcgtc catgtcgcct cttttgaaca agtaaaagag
    11041 tatgtggaca actttcatcc ggcagcagag aagattgtga agaacttctg gcctggacca
    11101 ttaactttga tatttaagat caaaaaagac actttgccct cagttgtgac gggaggatta
    11161 tccacagctg catttcgtat accggataac aaaaaaacac tagaagtaat cgaactttcc
    11221 ggtgttccgc ttgtcggtcc aagtgcaaat acgtcgggaa aaccaagtcc aacgactgca
    11281 gatcatgtat atcatgatct acaaggaaaa attaccggga ttattgatga tggagctacg
    11341 cgaattggtg tagaatccac tgttcttgat ttaagtgatc caacagccat gcctatgatt
    11401 ttacgaccag gtgcagtaac gaaagaacaa atcgaagcag tcatcgaatc tcctgttgct
    11461 attgatcagc atcttgtaaa agaaaatgaa acaccaaaag cccctgggat gaaatacaaa
    11521 cactactcac ccgatacacg tgttctgatg gtcagagatg gcgattggtc aactgcagtt
    11581 caatgggcaa aaaataagaa gatccgtgca ggtgtcatag caagccccga aattgcggat
    11641 caagtgcgga cagatactgc agctgtttat atgtataacg acaactcagt agaagcggcg
    11701 gctaaaggat tgtttgcagg tctaagagga ttagatgaac caacacttgg attagatttg
    11761 atttttgtac aagtgtatcc agaaactgga ttggggaatg cttacatgaa tcgcttgaaa
    11821 aaagcagcag gacaaaatta ttttgaaaaa tgatcaattg gttattgtcg cattctttta
    11881 gtatgataca atgctatcaa gtaaatcttc tatcagtgaa cgaaaaacta agcgtaacta
    11941 atcgtaatag aagagatacg aatatttggc aatttgtttt tgatgggaaa aataaaattg
    12001 taggaaaata aaaaggagct gttgtttgtg gtagattaca aaacgtttga ccctgatctt
    12061 tgggctgcga tcgccaaaga agaagaaaga caagaacata atttggagct gattgcctct
    12121 gaaaactttg tctccgaagc agtgatggct gcacaaggaa gtatcttgac aaataaatac
    12181 gcagaaggtt atccagggca tcgctattat ggtgggtgtg aatttgtcga tatcgttgaa
    12241 aatttagcga ttgaccgtgc aaaagaatta ttcggagcga aatttgcgaa cgtccagcct
    12301 cactccggct ctcaagccaa cacggctgct tatctcgctt tggtagaacc aggggacacg
    12361 atcttaggaa tggatctttc cgcaggcgga catctaacac atggatcacc agtcaacttc
    12421 agtggcaaaa cctatcactt tgttgcttac ggtgttgatc cgacaacaga agttattgat
    12481 tataatgtgg tacgtatttt agctagaaaa catcaaccaa aattgatcgt agcaggtgcc
    12541 agtgcttacg gacgaacgat cgactttgcg aaattcagag aaatcgcaga tgaagtaggc
    12601 gcaaaactga tggtagatat ggcacatatc gcaggattag tagcagcagg acttcatcct
    12661 aatccagttc cttatgcaga tatcacaacg actaccactc ataaaacatt gcgtggccct
    12721 cgaggcggca tgattttgac aaacgatgaa gcattagcaa aaaaaataaa tagtgcggta
    12781 tttcctggta ttcaaggcgg ccctctagag catgtgattg cgggtaaagc agtagcattc
    12841 aaagaagcat tagatcctgc atttaaagaa tatagtgaac aaatcatcgc caatgcaaaa
    12901 gcaatggtca aagttttcaa tcaagcaatt ggaaccagag tcatttcagg ggcgacagat
    12961 aatcatctga tgttgatcga tgttcgcgag ttaggtataa atggaaaaga agcagagagc
    13021 attttggaca gtgtgaatat cactgtaaat aaaaactcga ttccttttga aacactaagt
    13081 ccattcaaaa caagcggtat tcgaatcggc acgccagcaa tcactactcg cgggttcaaa
    13141 gaagaagatg ctgtaaaagt ggctgaacta gttgtcaaag cattacaagc aaaagatgat
    13201 aacgctcaac tagacgaagt caaaacaggt gtacgcgaat taactgaaaa atttccgttg
    13261 cataaaaaat aaaaatttaa attccattag taaacagaaa agttcaatgt tgggcttttc
    13321 tgtttatttt ttgggaaaac ggattctttg acaatatcgg tgatgctctc tagtatatga
    13381 ggtgtaattt tagtcttcac actaaatctc agggaggaaa agtcatgact gatcgtatct
    13441 cgttagaaaa ggaagcagta gcattcagtg atgcaaatgc gcctcatccg cgtatctatg
    13501 aactagcacc ggaagaagga agagttttac tagaagaagt acaagaatca cctgtgaaca
    13561 aatattccgt cgatattgaa gacttgatgg tggatactgg cgaatgggga aaactcaata
    13621 ttcgtctcgt acgaccagaa gggaatatgg ataaattacc agttattttt tatattcatg
    13681 gagcaggctg ggtttttggc agtgctcaaa cccatgataa attgatcaga gaacttgcgg
    13741 ttcgtacgaa ttccgtcgtt gtctttccag aatatagtcg atcaccagaa gcaaaatatc
    13801 caacagcaat tgaacaaaat tatgctgtgt tgcaacagct gaataaatta gccaagtcaa
    13861 aaaatctgga tctcgagaat ctaatcgttg caggagattc agtaggtggt aacatggcta
    13921 cagtcatgac gatcttaaca aaagaacgaa agggcctccc aatatccaag caattgctgt
    13981 attatccagt cacaaacgct gattttgata cagattccta tcatgaattc ggagaaaact
    14041 attttttgac aagagaagga atgatgtggt tttgggacca atatacgacg aatgaagaag
    14101 agcgtgctga aatcacagct tcgcctttga gagcaaatat aaatgaacta aatgatcttc
    14161 cttcagcaat gattttaacg ggagaagcgg atgtattaag agatgaaggt gaggcttatg
    14221 cgcgcaaact tcgtgatgca ggggttcctg ttacacaagt acgcttccaa ggaatcatcc
    14281 atgattttgt catggtaaat gctatggatc agacaaaggc aacgcgtgca gcaatggcat
    14341 tatctacttc ttggatcaag aaaaaagaat aagctttgct tgtccgcagg aaaacttctt
    14401 ttcaaaaacg gattttctta ggaaaaatgg cgagtttgac tagtcaatca cttgattttg
    14461 ttttacaatt aaaaagaaac gacagaacaa aggagagctt aaaaaatggg caaatttcaa
    14521 gtgatcgatc atccattgat tcaacacaaa ctaactatta ttcgggacaa aaactgcgga
    14581 acaaaggttt ttcgtgaagt agtcgatgaa atcgctatgt tgatggcata tgaagtatca
    14641 cgggatatgc cattggaaga catcgtgatc gaaacaccga tcactgaaac aacacaaaaa
    14701 acgttgtctg gtaaaaaagt agcgattatt ccaatcttgc gcgcaggtat tggaatggtt
    14761 gacggtatct tggaattgat tccagctgct aaagtgggac atatcggtct ttatcgagat
    14821 gaagaaacat tggaaccaca tgaatatttc gtcaaacttc cagaagacat tgatgcacgt
    14881 cagttgtttg tagtagatcc aatgttggca acaggcggat cagcaatcat ggcaatcgat
    14941 gcattgaaag aacgtggcgc gtcgaacatt aaatttgttt gtttggtagc agcacctgag
    15001 ggagtaaaag ctttgcaaga agcgcatcct gatattgaca tctatacagc ttctctagat
    15061 gaaaaattga atgaacatgg ttatatcgtt cctggactag gggatgcggg tgaccgtttg
    15121 tttggtacaa aataagtaaa tacgaaaaag ggtgtgacac aacttttgtc acatcctttt
    15181 ttggtcattt ttaacgtttg atgcagaaca gcttttt
//
LOCUS       DACDIE010000057        14760 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.057, whole genome shotgun sequence.
ACCESSION   DACDIE010000057 DACDIE010000000
VERSION     DACDIE010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14760
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.057"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..956
                     /locus_tag="IV250_002124"
     CDS             <1..956
                     /locus_tag="IV250_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8769380.1"
                     /translation="FVSEAYKLVEGKHLENEDKNKILMHKDLAEKNNLKVGDKIKIKS
                     NLFDADNEKGADETVEVEIKGLFDGHNSGVVSAAQELYENTLITDIHTAAKVYGNTED
                     TAVYQDATFFVKGDKNLDSVIKDLGKLDINWREYNLIKSSSNYPALQQSISGIYSISN
                     KLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLLSLGISKLEIFGQFLIEMVFISIPAF
                     VGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQSASSQLGGGAEAEGFNKTLSSLDIN
                     VLPKFMIYVVIFMSLVLLVSLIISSFNILRRNPKELLIDNN"
     gene            complement(1242..2864)
                     /locus_tag="IV250_002125"
     CDS             complement(1242..2864)
                     /locus_tag="IV250_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HAP8769381.1"
                     /translation="MMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFEFIYPIV
                     EATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYSFEEKIP
                     PFSTFGKNYVRRFRETTVFEDIFAYILEQAVKAGFVTEDNLYLDSTHIKANANKHKFT
                     KEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPESGYYVK
                     GEREKQFAYSVHTSCDDNGFILSTIITPRNIHDSQVAFQLVKQSKRLFPEINCVVADA
                     GYKTPKFVHFLTHLNLRPCLPYSRPKGKKELLSKNEFLYDEYFDCYICPQDQMLAFST
                     VTREGYREYKSNPKECVNCPLLNQCTISKNHQRVITRHVWGDLMDEVEHLRLTDLNKS
                     IYKKRKQTIERIFADAKEKHGMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKE
                     PLFFCSKIRNEVDKKLFQARVTSLEQLLSTVCDVGDISFSIRNQLRFKRSLPVSGNIN
                     VYFTKARFKRLIAITVSSIVMFLWLFRMGVIP"
     gene            3516..4487
                     /locus_tag="IV250_002126"
     CDS             3516..4487
                     /locus_tag="IV250_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAP8769382.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
     gene            complement(4565..4801)
                     /locus_tag="IV250_002127"
     CDS             complement(4565..4801)
                     /locus_tag="IV250_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP8769383.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(4770..4901)
                     /locus_tag="IV250_002128"
     CDS             complement(4770..4901)
                     /locus_tag="IV250_002128"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP8769384.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            complement(4977..5360)
                     /locus_tag="IV250_002129"
     CDS             complement(4977..5360)
                     /locus_tag="IV250_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAP8769385.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            complement(5393..6178)
                     /locus_tag="IV250_002130"
     CDS             complement(5393..6178)
                     /locus_tag="IV250_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HAP8769386.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            6356..7456
                     /locus_tag="IV250_002131"
     CDS             6356..7456
                     /locus_tag="IV250_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP8769387.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            7446..8270
                     /locus_tag="IV250_002132"
     CDS             7446..8270
                     /locus_tag="IV250_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphatase"
                     /protein_id="HAP8769388.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            8276..9082
                     /locus_tag="IV250_002133"
     CDS             8276..9082
                     /locus_tag="IV250_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8769389.1"
                     /translation="MKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLANYS
                     DIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLAANF
                     AGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYPSMK
                     EVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALTSSN
                     VNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            9079..9909
                     /locus_tag="IV250_002134"
     CDS             9079..9909
                     /locus_tag="IV250_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8769390.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            9916..10947
                     /locus_tag="IV250_002135"
     CDS             9916..10947
                     /locus_tag="IV250_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8769391.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(11054..12327)
                     /locus_tag="IV250_002136"
                     /pseudo
     CDS             complement(11054..12327)
                     /locus_tag="IV250_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            12604..12813
                     /locus_tag="IV250_002137"
     CDS             12604..12813
                     /locus_tag="IV250_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769392.1"
                     /translation="MDVLREYSSLFTEAYLLEQFGYKIPFADYQQGVGTAAELSFYEG
                     ISQAVGRPVDAEHFEQRISSTGKSE"
     gene            12863..13579
                     /locus_tag="IV250_002138"
     CDS             12863..13579
                     /locus_tag="IV250_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HAP8769393.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(13481..14001)
                     /locus_tag="IV250_002139"
                     /pseudo
     CDS             complement(13481..14001)
                     /locus_tag="IV250_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            14289..>14760
                     /locus_tag="IV250_002140"
     CDS             14289..>14760
                     /locus_tag="IV250_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8769394.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKC"
BASE COUNT         4348 a         2690 c         3241 g         4481 t
ORIGIN      
        1 aatttgtatc agaagcatat aaattagtag aaggaaagca tttagaaaat gaagataaaa
       61 ataaaatctt aatgcataaa gatttagctg aaaaaaataa ccttaaagtt ggagataaga
      121 taaaaataaa atctaattta tttgatgctg ataatgagaa aggtgcagat gaaacagtag
      181 aagtagaaat taaaggtcta ttcgatgggc ataatagcgg tgtagtaagt gcagcacaag
      241 aactatacga aaatacatta atcactgata ttcatacagc tgctaaagtt tatggtaata
      301 ccgaagatac agctgtatac caagatgcaa cattctttgt aaaaggtgat aagaatcttg
      361 atagtgtaat aaaagatctt gggaaattag atataaattg gagagaatat aatttgatta
      421 aaagttcatc aaattaccct gcattacaac aatctatatc aggtatttac tcaatttcaa
      481 ataaattatt ttttggctca ttaatatttg caggagttgt agtttcatta ttattattct
      541 tatggatgaa tgctagaaag aaagaaatag cagtattact atcattaggt atatcaaaat
      601 tagaaatttt tggtcaattc ctaattgaga tggtatttat atcaatcccc gcattcgttg
      661 gttcctattt cttagctcaa tatacagctg acaagcttgg gaataatata ttgaataagg
      721 ttactggtga tatagctaaa caaatagcta aacaatctgc atctagccaa ttaggtggtg
      781 gggcagaagc tgaaggattt aacaagacat tatcaagttt agatatcaat gtattaccta
      841 aattcatgat ttatgtagtt atatttatga gtttagtact tcttgtatca ttgattattt
      901 cttcattcaa tatattaaga aggaatccaa aagaattatt aattgacaat aattaaaatt
      961 ttcggtgctt tttagcacca aaaatgagag tgtaatataa accgtgtaag taaccatcaa
     1021 tcctaggcgg gtggttgctt cacggttttt atttttctaa aataagatca agaggtgatt
     1081 acacgatggc tagaaagaaa agaaatcctg atgctgagaa gttagctgaa tccattttga
     1141 atacttatca acccgaatct gtagaggata tgcaagatgt tttaaaagat gtgtttggtc
     1201 ccttatttga aaaaatgctt caaggagaat tgaataacca tttagggtat gacgcccatt
     1261 ctaaagagcc aaagaaacat gacaatcgac gaaacggtta tggcaataag acgcttaaaa
     1321 cgagctttgg taaagtaaac attgatgttc ccagagaccg ggaggcttct tttgaacctg
     1381 agttgattcc taatagaaaa cgagatgtct ccgacatcgc agactgtaga caaaagctgt
     1441 tccaaactcg taactcgagc ttggaacagc tttttatcta cttcgtttcg aatttttgaa
     1501 caaaaaaata aaggctcctt gcctttccat agccaggtgg ctaatttctt taaattcatt
     1561 gcagcaaaca ctagcatcgt atgcgtcgcg actttttcca agcctcgata cttggtccaa
     1621 cgcatcccat gcttttcttt tgcatccgca aatattcgtt caatcgtttg tttccgtttt
     1681 ttgtaaattg atttattcag atctgttagg cgaagatgtt ccacttcatc catcagatct
     1741 ccccaaacat gtctcgtgat gactcgttga tgatttttac ttatggtaca ttggtttagc
     1801 agaggacagt tcacacattc ttttggattg gatttgtatt ctcggtaccc ttctcgagta
     1861 accgttgaaa aagcgagcat ttgatcttgc ggacaaatat aacaatcaaa atactcgtca
     1921 tagagaaact cattttttga taatagctcc tttttccctt tgggccttga ataaggcaaa
     1981 caaggtcgaa ggttcaaatg agtcaaaaag tgaacaaact taggtgtctt atacccagca
     2041 tcagcgacaa cacaattgat ttcaggaaaa agtctcttcg attgtttgac gagctgaaag
     2101 gcgacttgac tgtcatgaat atttctagga gtgatgattg tgctaagaat aaatccattg
     2161 tcgtcgcatg aagtatggac agaataagca aattgttttt ctcgttcgcc tttgacgtag
     2221 tagccgcttt ccggatcagc tttacttatt tttctttctt tcagcttcac ttccttttct
     2281 aagtccaaag taaaagggcg ttttccagcc tctattcgtt gacgattgat ctcatcttct
     2341 aattcatcct gataggcttt cgcttcatca tgggtcattt ctttcgtaaa cttatgttta
     2401 ttggcgttag ctttgatgtg ggtagaatcc agatacaaat tatcctctgt tacaaaacct
     2461 gctttaactg cttgttctaa aatataggca aaaatatcct caaacaccgt cgtttctcta
     2521 aatcggcgaa cataattctt tccaaaagtt gagaaagggg ggattttctc ctcgaaggag
     2581 tagcctaaaa accagcggta agcgacattg gtatcgactt ccttaatggt ttgtcgcatg
     2641 gaacggatac cgaataaata ctgaataaac accaatttga tcagaatcac tggatcgata
     2701 cttgggcgac ctaaatcgga gtaagtggct tcgacgattg gataaataaa ttcaaaatca
     2761 agcgcggcat caatctttcg aacgagatga tcttgaggaa ctagatcatc aatcgtaagc
     2821 atcgcaaatt gatttctacc attcgtattg tttttagaca tcatagctat cactccttgc
     2881 attcgttttc ataaatctat tataaaagaa aagactgtag acatcatcca taaaatggac
     2941 tttgtctaca gtctggataa cgtgattttt tgctgtaatg acaaggtttt agctgtttca
     3001 gataccatgt atccaaaaat agtttattga gaaaatagga gagtatttga tactaactag
     3061 taggaaaaaa tgagataaaa agtaaatgga atcatttaag gtaatctgaa ataaaaaata
     3121 tacagccctg tggtacttta cctacaaggc tgtatatatt ttttactctc agcgtttttg
     3181 actccgtgtc tgactccgtt ttattgattt tgaatgttat tcaatgacca tttaacaggt
     3241 caaatttgat gtttttgatt ccgtatgacg ccgtttgata tctagtgata tagtgatttg
     3301 agtctacatc gaataaggga ataaaccaat aaaaaatgat ttagaatatt aaaataacaa
     3361 aaaagaggac tacctgtatt agtcctcttt tttgaccaga ttttgaccag tctattttac
     3421 atcctagtat atttatatga gctaatttta tgattaaatg gtaagttttt ccttgtgtgt
     3481 tttagtttaa actagtgtgt aattcaaaaa tctatatgga aggaacagta ttaagaatag
     3541 aacagggttc tctacatgat ggtgctggtt tacggaccgt tgtttattta aagggctgcc
     3601 cattacgctg tgcctggtgt tcgattcccg aatcacaaag caagcagatt gaaaaaggat
     3661 ttggccagac catgaccgct gaagaggtaa tggatgagat tgagaaggat gctgtttttt
     3721 actttcactc ggatggcgga gtgaccatta gcggaggcga ggcactggtt caggcggatt
     3781 ttgctaaaga aattttgcaa aaatcgaaat acattggaat caataccgta ctggaaacca
     3841 gcttttgtgg tgcttataat gaaatccaaa aggtagctcc ctatgtggat acattgtttg
     3901 tggatgtaaa aatgtttacc agcaagctcc acaaacaatg gaccggctta gataaccaac
     3961 aaattttgaa gaatattcgt cgctttttaa ttgagtatcc taactgcgaa gtgcgaattc
     4021 gagttcctgt ggttccaggt atcaatatga acctgaccga gctgctgacg attgcttgtt
     4081 ttgtggcaga tcttgatcgg ttcgtaccgt tagaattact accgtaccac tgctatggta
     4141 tgcacggtta ccaagcattg ggactggagt atccgttggc agacacgcca gcgccttcac
     4201 ctaaagaaat gtttgcattg gcagatcaac tggctcgcac ggttcctcat ttgctggtca
     4261 cagctttaac caaaacattt atttatttat tgaatccaag ctttatctta tggataaatg
     4321 aaagacactc actagctgca gccagctcaa gaatagagct tttgctgaag tcagtgagtg
     4381 tctttttacg atgttttttc gaagttaatt tgtcgaatga acactggctc aatcggtaca
     4441 tcttcactgt ttgtcggctg agctgcgatc gctgctacga tttctaaccc ttggtatacc
     4501 tgtccaaaaa cagtataacg accatccagc tccggtagtc ctccaagctc caagtatgct
     4561 tgctttacat tagcaggtca gcctgcgcct ttgtgttttg cacaataaaa aactggttgc
     4621 cgttggtgtg ggggccgtgg tcccctaagg ccaaggcgcc attaaaatga taaagtccgc
     4681 gatgcacttc atcagcgaaa aagccctcat agctggaaac agcttcatca ccagaaccat
     4741 ccaaagcgcc accttgaata acaaattcct taatgacacg agccgaaagg tgtgttgtca
     4801 taaacgcctt gctcagctag ggagcgccag ttggctacag ccaggggagc ttggtcagga
     4861 aataagcgga gacgaatttc accagcatcg gtgaccagca cacaaacttc ttcctgctgc
     4921 ggtttttcta attgaaagag aggaaggttt gcaactgaaa cgccttgatc ctgccatcaa
     4981 atatcgaaaa tccgccactc gaccttcgcc tgatcaccaa ggcgagccat tcccaataaa
     5041 acggtatagg cggtttcatc cgcttgaaag gtcaattgca gctgtcctcg taaatcgttg
     5101 tagtcatctg gggcggctac ttgataaaaa agatgaaact gcgcatgcaa atattccaaa
     5161 ggctgatctt taaaggctgc aaaataagcc tgaacttttt cccgctgcaa gctgtcagcc
     5221 accaaaaaat tttccagttg ttgttttgga acggctgcta acaaggatcg gcaaaccgcc
     5281 tcaattttca acgcatccca atgtcgcgca attaaataat ggatgggtgc ctcttgattg
     5341 tagtcccaca ggttgctcat ttttttcacc actttcttct attatatagg aatcacaaaa
     5401 tcggcagcgg cgtcaggcgg cgttcttttc cttgaatgac tagcaacttt tctacctgac
     5461 agtctgccaa cagctgactc atttttttaa agcccagctg ataatcttcg gcaacatgag
     5521 catccgagcc taaggtaaag agctttccgc cgacactttg atacaaagga acggcataac
     5581 ggtaaaggtc ttcgttgccg tatttcaaga agctcttggc atttaactcc atcgccaatt
     5641 ctcgttcaac aacctttttg aaaatcgtta gcagctgagc ctcaaaatgc tccgcgaatt
     5701 cttccgctgt gaaggtgaag cggcgtaatc cataatcaaa gtgagtcaaa atatctcctt
     5761 ctggaaaatc cgtcaagacc tgcaacatct gatcaaaata ttccttggta gtttgaatgg
     5821 gatctttggt taaaaccaca tcgtccatat aatcaaagcg gccattttga tgaatactta
     5881 ccagctttaa atcataagga tgctgagcga ggtaatccac gatccgctct tcttgtcctg
     5941 gaacaacgcc aatctcgata cctcgcagaa atttggtgga ataggtttct tttaaagagt
     6001 ccagtttgtt tatataggct ggataatccg gaatgtcatc ttttccggta gatggattgt
     6061 ttaaatcaaa gtgatccgta gaaacgaaaa actccggctg ataggctaaa tagttttcaa
     6121 atttttcctc tgaatcaaag gaaaaatacg tgtggagatg ctgatcataa tactgcatag
     6181 ggagcctcct tttttctact atcataccgc aaaagcttta gaaggttaac gggaattcgt
     6241 cgaaatttac aaaccatata caacctttac attgttcatg aaaccttttc aagaaactga
     6301 ttctctcatt acaaagcttt gttagaatgg aattatcgaa aggggagaag aatgaatgaa
     6361 aatgaatgaa aaaattcgta acatgactat cggtttattt tgtgcagcaa cagtaagtat
     6421 cctgctgaca ggctgcggct caggtgaagc aacgaagcaa tcggctgcgg cagacgactc
     6481 tttagatacg attatagaaa aagccaagga agaaggagag attgccagct tgggaatgcc
     6541 ggatacttgg gcaaattggg taggcacttg gaacgacctt gaaaaagaat acggcttaag
     6601 tcacgtggat acggatatgt ccagtgcaga agaattggct aagtttgaat cagaaggaaa
     6661 aaatggaacc gccgatattg gtgatgtcgg tatcagcttc ggtccgttgg ctgaaagcaa
     6721 agagttaacc ttaccttata agaccaacta ttgggaggaa attccggatt gggcaaagga
     6781 tgatgatggg gattggttgt taagctatac gggaaccatt tcctttatca ccgataaaga
     6841 gaatgtgaag caagcaccga ctagctggaa ggagctgcaa gagggtgact atcaagttag
     6901 cgtgggggat gtgacgattg ccaaccaagc acaatttgct gtattggctg cggcgattgc
     6961 caacggaggt gacgaaaagg atattcagcc aggtctgaat tactacaaaa aaatggcaga
     7021 aaaaggccgt ttgtccaccg ttgatgccag cgtagcaaat cttgaaaaag gtgaaatcga
     7081 tgtggcagtt gtttgggact tcaatggact aaattatcgt gaccaaattg actccgagcg
     7141 ttttgaggta actgtgccaa cagacggctc catcattagc gggtatacaa ccttaatcaa
     7201 taaaaatgcc cctcatccca atgcggcaaa gctagcacgg gaatatattt tatctgatga
     7261 agggcaaatc aatttggcaa agggctatgc gcgacctatt cgcaaggtag agctgcctaa
     7321 agaagtagct gaaaaattac tgccggagga tgcttatgaa gcggcggtgc cagttgaaga
     7381 caatgaagct tggaacaaga cctctgtaga gcttccagag ctttggcagg cggaggtact
     7441 gacctatgca aagtagactt gacaaactga ttgtagttat actagacggc tgtcgctacg
     7501 acacagccgt cgcccagctt gggttcatga atcatttagt tgagcaccag caagcaagct
     7561 tattgaaggt aaaaagtgag ctgcccagca attcacgtcc gttatacgaa gtactgatga
     7621 ccggcgttcc agcctatgaa aatggcatct atagtaatta ccatgctcag cgatccaaag
     7681 agtggtcgct gtttgaatta gttaaggaag ctggcggaaa aacaggagcc gccgcttacc
     7741 actggtacag cgagttgtac aatcaagccc cctataatcg tttggttgat cgcatccagc
     7801 atcaggagga gcgactgatt cagcatggca tcttttatag tgaggacact taccctgatt
     7861 cgcatttatt tgcggatgcc cattatttag tgaatcagta tcagccagac tttttggtcg
     7921 tacattcgat gaatattgat gatgtcggtc acaaatttac cgccgattct catgaatatg
     7981 cagcagcggt taatcaggcg gacagattat tggctattta ccttcctgaa tggctgcaaa
     8041 aaggttatca agtcgtggtg actgcggatc acgggatgga tgcgcacgga ctgcatggcg
     8101 gcagcttggc tgcacatcgt gaagttcctt tttatttgat ttcaaataaa cgccaaaagc
     8161 tgaaggaaca aactgtacca cagctgctgg cggctccatt gttttgctgg ttattaggaa
     8221 ttgcaccgtc agagaaaatg caggacattc aagaattgat gaggggatga aagagatgaa
     8281 aaaacgcttg gttctggttc ctgcctcctt tatcttctgt gcatttttac tgctgccgct
     8341 gctttttatg gttctgtgca gcttgcacag tgatttaact aaccagtgga cattggccaa
     8401 ttacagtgat attttcagca acaaatatta cacacaggcc tttgttaaca gcgtactgat
     8461 tgctgtcagc tccagtgtga ttgggttaat cgggacgttt attttatgtt tatgcttaat
     8521 gaatctatcg actaaattgc aggagaaaat cacctttgtt tccaatttgg cagctaattt
     8581 tgctgggatt ccgctggcat tctcttttat tattttgttg gggaatgtag gtgtgctgaa
     8641 aatgattttg ccatttctag cggacttcaa tttgtattct tggtggggat tgaccatcac
     8701 gtatacctat ttccaaattc cgttaggggt cttgtttttg tatcccagca tgaaggaagt
     8761 gaaaaaggaa tggctggagt ccgcacaatt gctgggcgct tcatcagcgt atgcttggtt
     8821 caaggtggct ttgccttttt tgcgaccatc gattgccagc acctttgtca tcttatttgc
     8881 taatggcatg gggacctatg aaacagcgta tgccctgacc agcagtaatg taaatctttt
     8941 aacgattcga attgcggctt tagtgtcggg agatgtgttc gccaagccta atttaggcag
     9001 tgcgttggca gtcgctttag gtgtcctgct ggtaacggca atgttgatta ttcaaaagaa
     9061 aggcaaggtg gtgcagacgt gaaaaagtta ataaaaccga ttttgttctg ctttgtcctg
     9121 tattacttgc tgccgattat tggaacgatt ttgtatgcca gctccactaa atggtcaaaa
     9181 tcgcttttac ctagtgattt taccttgcaa tggttccaac agctgctgac ggacagggaa
     9241 tttattgctg cggtgggtcg ctcactttta ttagcaggtg tggtcttggt gacgattctg
     9301 ttgttgatga ttcccaccat tatttggatt catttgtatt tccctcgttt gaatcgttgg
     9361 ctggaaaagc tgctcctgct gccgtatgct ttaccaggag tgattttggt aacggctttg
     9421 cttcgaacgt atgctgaaac tggtatccca atgtttgtag tgttggtagg tgcattgttt
     9481 attacggcgt tgccgattgt ctatcttagt cttaacaacc agatgcgctt aattaatctg
     9541 aaggaattag tggatgcagc agaaaccttg ggcgccccga tgtctacgat tattatccag
     9601 gtgctgtttc caaacatccg tatcggtgtg accctagttt ctctgatgat tttttcttcg
     9661 gtttttgggg agtacatgct gacgaacctg ttgattggcg gtcgatttca gacggtacgc
     9721 atctatatgc tgcggcggat gaacgaaaac ggccatttgg ctagcgctgt gatggttttg
     9781 tatttgctgt ttatggtggg aattgcttta actaccttta tgctgactta tcgacaaaag
     9841 caacagctgt cccttaagca gacgagggaa gagaagcagt cgaaaaagaa gcccttaaag
     9901 gagttgtgac ggaaaatgtt tttagaaata catgatctca atctgttttt tgatgaaaaa
     9961 cagattttag ctattgatga aattcagatt gaaaagggtg agctggttac tttactaggt
    10021 cccagcgggt gcggaaagtc tacgttgctg cgctgtatca cgggattgga acaaccaaag
    10081 agcggtgaga ttgtgctgga caatgagaat attgccgata agcctacgaa ggatcgcaat
    10141 atcggctttg tttttcaaca gtatgcattg tttccaacta tgactgtttt tgaaaatgta
    10201 gcctttggtt tgaaggtaaa gaagctgagt cctgaggtga ttcaggaaaa ggtttttgag
    10261 atgctatcat tggtggatat gacggagcag gcagataaaa atgtccagtt tctttctggc
    10321 ggtcaacgac aacgggtggc actggctcgc tcattggtca ctgaaccgaa agtcctgctg
    10381 ctggatgagc cattaagtgc tttggatgcc cgaattagaa aacagctgca gcgggatttg
    10441 cgcgctatcc agcaatcgct agggatgacg atgatttttg tgactcatga ccaagaagag
    10501 gcgatgcgaa tcagtgatcg gatttttgtg atggaagctg ggcgggtggc tcaggtttcc
    10561 acctcgaagg aattgtatca gaatcctcag agccgatttg ttgcggaatt tatcggcaac
    10621 tataatcgtt tcgaatggta tgagctgaat cagtataccc acgagttttc cgctaagggc
    10681 aatcgctgca tttattattt aagaccggag ctgattcaat ttgaaccggt tcctcagggg
    10741 gttaagattc cggttctatg gaaggaatca tttattttag gcaacattca acggtatgtt
    10801 tttcaaacgc tggagggcaa agaaatttta gtggatcggt tgaacgatcg ggaagagaaa
    10861 attagcgatg ccttatacat tcagctgaaa gatattctag caatccctgt ggaaattgat
    10921 actcagcatt ttgcacaaca aagctagtgt atagtcggca aacatttctg gctctttgtc
    10981 aaataggact gatagagtga tataagaaca tctaaggtag aattgatttt ctgctttaga
    11041 tgttttttta tgtttaataa gtgatcagat gattgattaa gaaaattctg gttctcatcc
    11101 taacccgtaa gctattcgtt tcaatgcttt gatgtgggta tgcagatttt ctattttgcc
    11161 atttgagtaa ggcaactcta aggctaaacg aatgccggat tcatagttta taaacgcttt
    11221 ttttatatga tgcagttctc ttggaaccga cttgggcatt gtttttacaa gctgaaagaa
    11281 gatgtccgct tctttgtcat aaaaagcttg aagaatttct tgatagtaag cataagctat
    11341 tcgtagttca tcagaagtag aaagtagacg ttctacgact tcactttcgg ttaaataggc
    11401 ccaaccaaaa ttacgccatt ttgtccattt atcataaaca agcgtgtcct gtcgtttgat
    11461 cagaagtcga gataaagcct tcacttgtcg atagtgtttg gcttgtattg gatcgtttcg
    11521 gtcgaagctt ttcatgactc atagtcaaat atccttaaaa ctgcgaatga ggtgcttgac
    11581 gatgtgaaag cgatccacaa cgagtttggc atgaggaaag cactctttta tcagcaaagc
    11641 ataagaggcg ttcatatctg ttacgagtat ttttacgttt aaacgtgcac gtcgttcaaa
    11701 ttgcataaaa taagaaatga gatctttctt cttacgtgaa ggaagaatat ccagtaattt
    11761 cccagtatcg ccatccatac aagaaaaaga atagggaccg acttgatttt tcaatgaacg
    11821 aaattcatct accaatgaac agcgaggcaa ccaatttatt ttcacgtttg tcatggggag
    11881 cagagaagcg agtgttcgcg aaacggttga ccaagaaacg ccgcaatgtt tagaaatcgt
    11941 cgtcattgct tgtttttcag tcaattcctc caatatttta taatgtactt gtttggcgat
    12001 aaaacagttt ttttcaacaa tgtaggtatt cgccgtaaaa taggtgtgac agccacgaca
    12061 gaggtaccgt tgctttgcca aagcaagata agtccgttga ttcgcacact tattgagaag
    12121 gaccagcgat tttttctttc cgttcttaac gatcagccca tgttgattca cctgtttaca
    12181 ctgaggacag ggctgaatcg ttttaatgag cgtggcagaa acaacgatac tgttgattga
    12241 atgtattttt ttcttctcga catgagtgat ttgaatgttt ccatctttga ttcctagtaa
    12301 tagtttgata agatgaagat caggcataca gaacgctcct ttgattgtgt tgtggtgatt
    12361 caattctaac ggagcttctg tatgttttgt ttttttatgc tcaaaaggac tgatagaatg
    12421 atttactatc agtcctagaa attatagcgc cacatttctt tcacctttga cttttcagac
    12481 ggcagttttt atgctgatac tgtagagaga gcaggacaca gcttttgctt gttttgggta
    12541 agggggaaag aagatgacca aagcacttat ttttgatttt gatgggacga tgattgacag
    12601 tgaatggatg ttttacgaga gtattcatca ctatttactg aagcctattt actggagcag
    12661 ttcggctata aaattccttt tgcagactat caacaaggtg ttgggacggc agcagaactg
    12721 tccttttatg aagggataag tcaagcggtt gggagaccag tggatgctga acattttgag
    12781 cagcggatca gcagcactgg aaaatcggag tagtctcaat tctgaactgc gggagctaga
    12841 ctattttttt gaagcacatc cgatgattaa ggaaaaaatt gattcctttg tgacgatcga
    12901 tgatcttcag gtcggcaaac cagatccaga aggttatttg ttgtgcttga aacggctgag
    12961 ggttgaacag gctgttgtag ttgaggatag tccagtcggt gcccaagcag caccgcatat
    13021 gacagttgat gtacaagcgt tggatgcaga ttttttcgcc tttagcgggc ataaaatgtg
    13081 cgggccgaca ggaatcggtg tattatatgg caaacgtcaa ttgttagaac aaatggagcc
    13141 agtcgaattc ggcggtgaaa tgatagattt tgtcaatctt tacgatagtt catggaaaga
    13201 gctgccatgg aaatttgaag ctggtactcc gaacattgca ggagcaatcg attacttgaa
    13261 tgagattggt atggagaata ttcatcgtta tgagcaagaa ctggttgcct acgttttacc
    13321 taaattacaa gcgatcggcg gtatcaccat ttacggaccg caagatcctg aacatcatac
    13381 tggcgtggtt gcttttaatt tggatggcat ccatccccgt gatgtggcga ctgctctgga
    13441 tatggaaggt gtcgctgtac gttcaggaca ccattgtgca caacggttca tcgacgattt
    13501 ctgtttgact aattggtcgt ttaaacagtc gatgataatg ccgtttgagc ggctccaatg
    13561 tcgtggaatc tttgagtaag agtttccaat atcgcttgag ccgacggtac ttcttctgat
    13621 tttcagatta cgtgattgat tttcgtacag ttctatgaga atcgcctgct tcagttcctt
    13681 tgttagttga tgatgttcat caactaagtc cgttttcgca gaaaaggtct gcccacaatc
    13741 atagcataga aaacgggtac gttttaattc taaaatagtc aacttttctt tgactttcaa
    13801 catctgagta cgtgtcgtat aggaaccgtt gcggataata cgaatcgatc cacagtgttc
    13861 acaatgggaa ggtgagtcat ccaatctccc tttaataaac tgcgcttgaa cgcctttata
    13921 ttctttttca atcagccatt cttcatcaaa aaataaatgt ttatctgtta atccgagtaa
    13981 ttttctagta tgattatcca tagagcgact cctttggtag ggtgatgttt tggtcgacta
    14041 aattttacca aaagtgagtc tctttttgag ttgaaacgaa gaaaaagtgt tagtagaaaa
    14101 tcgccttcga ttttccaaca acacaaaata ttatagaagc cttttttcta aaggatttca
    14161 aggtgtctgt ttgcatttat taaaaaaaag aaaaagtatt ggcaacagaa ggcgtcacca
    14221 acactagata ttatagagcc ataatatgct gtataatatc tagtgtatag gcttaagtgg
    14281 aggtatatat gaaaatatta actgttgaag atgataatat gataagagag ggaataagtg
    14341 aatatctttc agaatttgga tatactgtta ttcaagctaa agatggaaga gaagccttgt
    14401 caaaatttaa tagcgatata aatttggtta tcttagacat tcagatacct tttataaatg
    14461 gtttagaagt gttgaaagaa attagaaaga aaagcaattt accaattcta atcttgactg
    14521 catttagtga tgaagaatat aaaattgatg catttactaa tttagtagac ggatatgtag
    14581 aaaagccatt ttcattgccg gtcttaaaag ctagaataga ttctttaatt aagaagaatt
    14641 atggacattt agagaagttt gaatataagg atctatcggt taattttaat agctatacag
    14701 ctaaaataaa tggtgaagaa atagatgtaa atgcaaaaga actagaagta ttaaaatgtt
//
LOCUS       DACDIE010000058        14614 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.058, whole genome shotgun sequence.
ACCESSION   DACDIE010000058 DACDIE010000000
VERSION     DACDIE010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14614
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.058"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            506..838
                     /locus_tag="IV250_002141"
     CDS             506..838
                     /locus_tag="IV250_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8769395.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            1134..1289
                     /locus_tag="IV250_002142"
     CDS             1134..1289
                     /locus_tag="IV250_002142"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAP8769396.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            1565..2905
                     /locus_tag="IV250_002143"
                     /pseudo
     CDS             1565..2905
                     /locus_tag="IV250_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(3146..4762)
                     /locus_tag="IV250_002144"
     CDS             complement(3146..4762)
                     /locus_tag="IV250_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP8769397.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            4880..5098
                     /locus_tag="IV250_002145"
     CDS             4880..5098
                     /locus_tag="IV250_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8769398.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            5293..5754
                     /locus_tag="IV250_002146"
     CDS             5293..5754
                     /locus_tag="IV250_002146"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8769399.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5947..6186)
                     /locus_tag="IV250_002147"
     CDS             complement(5947..6186)
                     /locus_tag="IV250_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769400.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(6210..7733)
                     /locus_tag="IV250_002148"
     CDS             complement(6210..7733)
                     /locus_tag="IV250_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP8769401.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            complement(7751..7873)
                     /locus_tag="IV250_002149"
     CDS             complement(7751..7873)
                     /locus_tag="IV250_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP8769402.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            complement(7903..8886)
                     /locus_tag="IV250_002150"
     CDS             complement(7903..8886)
                     /locus_tag="IV250_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP8769403.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(8910..9059)
                     /gene="rpmG"
                     /locus_tag="IV250_002151"
     CDS             complement(8910..9059)
                     /gene="rpmG"
                     /locus_tag="IV250_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP8769404.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(9080..9457)
                     /locus_tag="IV250_002152"
     CDS             complement(9080..9457)
                     /locus_tag="IV250_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769405.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(9489..9668)
                     /gene="rpmF"
                     /locus_tag="IV250_002153"
     CDS             complement(9489..9668)
                     /gene="rpmF"
                     /locus_tag="IV250_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP8769406.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(9683..9952)
                     /gene="rpsN"
                     /locus_tag="IV250_002154"
     CDS             complement(9683..9952)
                     /gene="rpsN"
                     /locus_tag="IV250_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP8769407.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(10475..12247)
                     /locus_tag="IV250_002155"
     CDS             complement(10475..12247)
                     /locus_tag="IV250_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8769408.1"
                     /translation="MRMQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINM
                     VPAFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLR
                     VQIADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVM
                     LLVGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEI
                     NSFNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLL
                     TGEVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSP
                     LKSFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDY
                     DEGCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAA
                     RLANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLD
                     AENEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSE
                     IYKNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(12201..13955)
                     /locus_tag="IV250_002156"
     CDS             complement(12201..13955)
                     /locus_tag="IV250_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8769409.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLIKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(14065..>14614)
                     /locus_tag="IV250_002157"
     CDS             complement(14065..>14614)
                     /locus_tag="IV250_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HAP8769410.1"
                     /translation="FIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGLLSGIIFV
                     YISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVFGYDRAIP
                     QVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGIWGIISVL
                     VVASLSMIGVLIGRKVLKKYKK"
BASE COUNT         4679 a         2836 c         2232 g         4867 t
ORIGIN      
        1 agggagtctg tgacataagt aaaaagatag tttcaaattt cgaacaataa atttgaaact
       61 atcttcttta taattataaa gtatcgattt tacagcgttc tataatgatt catagaatct
      121 aaaacgaatg attttaatta tcaacgtttt ttaattcgtt tttagcttaa aaataacttt
      181 tgtcacagtc tcactcctaa aattacttta tttacgtatg aagtgggcag gaggtcgaat
      241 ccagaactgg gctatggtac ttcaacgagg acttctcctc tagaattgaa aagcatgcga
      301 tttaccttcc gtaacgacgg tagatttatt caactatgtg tccaatcaaa atttgtctga
      361 tttcaaaaat agtcaagaga attttggttg ttaaaaactc ttatctatca acttccacag
      421 tttagttgac aaaccctgat actaagttgg gctatgatac acttttcttg actatcagtt
      481 acgttaccgg aaggaatgaa ctctattgag aaaaacatat gatcgtaaat ttaaactaaa
      541 gatttcacag gatatccttg agaaaaaagt aactaccaag aaaattgctg aagaatacaa
      601 tatctctcgt ccaacaattt cccgatgggt ttcagaatat cgtcgatacg ggaaaaatgc
      661 ttttgctgga caaggaaaaa gattacctga taaagcagat ttctacattc ttgaacagga
      721 aaataaacga ctaaaagagg aagacgagtg ttctacgaac acaaagatcg atacggatct
      781 ccagggatca ctcaaaaact ttacgatgaa ggaatcgaaa tcaataaacg tgttgtagct
      841 gttcttatgc gtgaaatgaa tttatgcaca agaggatttc atcgacgcaa atcttctcat
      901 ggtagagaaa aaacaatcaa agaaatggta aaaaaatgtt gaatcgataa tttgaacaga
      961 accaaattga tgctgtttgg gtgatggata ttacttacat tccatgtaat gacggtcgcc
     1021 tctatttgtc cacctatatt gacttagcaa ctcgtattct acgttactat atggtagatt
     1081 cacacatgaa aaaagagatt gtcgtccgtc cattacaaat ttattaagga gaattgccta
     1141 cggtaattca ttccgataga ggtagccagc atcggtcttt tatttatcaa gagctgttga
     1201 acgaccacca catcacacat tcagtgagcc aaactgggac tcctgttgac aatgcagtaa
     1261 ttgaagcgtt tcatcgatca atcaaatgag aattaatcga ctccaacaaa tataaaagta
     1321 aaatagagat gaaggtgtta atccaagatt atttagaaga ctactaccca aacaaaagaa
     1381 tccatacaaa atttatgatg actccttgac aatttgaagc gaagaaaata agggatctat
     1441 aatatctagt gttggtgacg ccttctgttg ccaatacttt ttcttttttc taataaacgc
     1501 aaacagacac cttaaaatcc tttagaataa agtcgacgaa aaccattcaa aggagtgttt
     1561 caagatgtcc tattcagagt ttaccaaaga gttactagat attctagacc taaatcttat
     1621 ctttcatgaa gatgcctttc gcaaagagcg aatcaatgac gaaacatgtt ttgtttttga
     1681 tggcacattg acctatcaac cggaggaatg ctttcatcgc cactatcaaa atgaacaaac
     1741 gatcattaaa tagggctgga aaaaagtatc catcttattg aatgatgtca gtaactacaa
     1801 gaccattctg cggattaaca aacagcgctt caaatgtaaa cactgtggaa agactttttt
     1861 agcagaagat tcagttagtg atcgtcgctg ctccattgct cgcagagtca aacaagccat
     1921 tcttgagcta ttaagcgaac ctctttctat gtctttgatc gcacgtatga agcacatttc
     1981 tccgaccact gtcattcgaa tacttcgcag ccttcggcct aaaaccattt ctttgagcca
     2041 gctgttacca gaagtggtgt gtttcgatga attcaaatca gtgaaaaatg tctcgggagc
     2101 catgagcttt gtcatgatgg acggcaagac ccatcaactg attgatattg tggaaaatcg
     2161 gcaactcaat cctttacgcg actatttctt gcgctaccct ataaaagtcc gtgaacaagt
     2221 gcgtctagtg gtttcagatt tctatacacc ctatcgcaca ttggtgaaag agtgttttcc
     2281 gaatgcccga atcgtcgccg atcgctttca tattagccaa cacattggtc gggcttttac
     2341 caaccaccga attcaagtaa tgaagacctt taaaaaaggg gatcgtcggt cgaagcactt
     2401 aaagaaatac tggaaactac ttcaaaagaa tgcgtgggaa ttgaaaggtc agcatcgcta
     2461 ctggcgtccg tcatttcgag atcatctgac agaagcagag atagtcgatc ggctattatc
     2521 ttatgatgat tccttaaagc gaggctacga agtctaccag gattttcttt ccgttatcag
     2581 aagacaagat gttcctgaat tcgtcgcgct cctaaaagaa gactacaagg agttgccaga
     2641 gcactaccaa ccagtgttca cgaccttcaa gaaataccaa acagaaatca aacgagcgtt
     2701 gcgcgttccc tattccaacg gccctataga atgtttgaat aaccatatca aagtactaaa
     2761 acgaattgcc tatggctata gaaactttca aaactaccga gaacgaatct ttttatatcg
     2821 aggaaaatat tttaagaaaa ctaagaatac tacccaactg actaaagcca gaacaacaac
     2881 acggctagac aagatagctg tctagccgtg tgagtatctc gattattcta ttgaatttct
     2941 tcaccaacac tcattgacga agagccaaaa taagtaatct aaccgactgt taactaaacc
     3001 attgcaggtc ccgttaaaga tacttgtgcc tgttgtcacc gcttcttttt cttgtccaca
     3061 agcgcttaac acacttaata aagcccctgc tattattaat aataatatct ttttcttatt
     3121 aaaaacctcc tcatctattt ccagattagt aaatgtagta atctgtgctt tcttttttct
     3181 caatactaat aactaactta tggggtaaac aaatactcgt ttgaccgggt tttgagatcc
     3241 atccagttct aaccgcgatt tgatcaggac tattatcttc tttaactcga attcttccat
     3301 ctttaatctc aacaatatta tattgtccct tcgcaggatg ataattcacg agtttatgat
     3361 tgatcttatc caaatcgaag cgatccattt ccttgccatc aattcgaacg atggcatatt
     3421 tgatagcatc ttcagttgtt tcattctcct tttctataga gaatacaaat aagggaataa
     3481 aagaggtaat aatcaaaatg ccaataatta tgacatcgcc tatcttgaca ccagcttttc
     3541 ttaaaaattt cattaacgat atctggctaa acatattaga ctttttccat ggaaatggtg
     3601 aaatttttcg gaaattatct gcattaagag aatcagttaa acaaccctca agttgttcct
     3661 taatacattg ttgatccatc ttcataccaa taaaaactag tttattaatg cgttcaccaa
     3721 acaacggatg ccaggcttcc ttcaattcgg gatttttttg tataatcatt tgacactcaa
     3781 ttttaggtaa actagctacc caataattta atgattcaat acttttatag ctacccgatg
     3841 tttcaaagat cgtcgaaaaa tcactatgtt gggcaaacca aagaaaccct tttgctcttg
     3901 taatattgcc aggaaaattt ttatccgtaa gatgggcaaa tttttgtgcg tcaaggagta
     3961 accgatttcg atagataaaa cttgagatac catattcttc tgtttcagga atatgatttt
     4021 gatagccaag ctctaactct ttgaaccagc ccactgaata ggaagtctca tctatattaa
     4081 aaagatttgt atttagaatt ttcgataagt ctacttcacc tttaattgtc ctaattattt
     4141 tggcattgga ctgtaactta gcaattagac cgttaatttt gtaaatcgtc ttggttgaca
     4201 ctaagtcact attatttaaa agtaatatgt tacaacactc gaattgatca attaacaagc
     4261 cacgaatatc ctgttcttct tcaactacat taccttcagc ttttctttcc tgaatctttt
     4321 cacccgtttc gaaattatcc caaaaacgtt ttacatccac tactataata atggcaccaa
     4381 tctctaatcg ttgggtcaaa tcaatgtcta aagggttttt agctaatata attgcttgaa
     4441 taattggaat tggtttgctg attcctgaag cttcaataac aatttgattc aagtcagaaa
     4501 attgcactaa ttgatctagt tcaacaatca aatcttcgcg taatgtacaa caaatgcatc
     4561 cattcggcat cgaaacaagt ttttctccag ttcttttaaa acctttcttg tcgactaata
     4621 ccccatcaac atttacttcc cctatgtcat ttacaatgac agcaatttcc tttccgtgat
     4681 caccgtacag aatatgattt agaagtatcg tttttcctga accaagatag ccagttaaga
     4741 ttgtaatagg tatttttttc atagggaacc tctttctata ccttgaattt acactttaag
     4801 tgcaacacat aaaaatgagt cataatcgat atccaactag tataagtttg tcgagaacac
     4861 tcctagaagg aaaataatta tgagccagta tcgtcatctt actatactcg aacgagaaag
     4921 aatctttctg cttcacgaag aaggattttc tattagtata attggcaacg acattcagca
     4981 tagcccgttt actattttac atgaactgag aagaaacaag aaaaaaacta ttcaccttcc
     5041 aaagcggaaa aaaattatgc ttatcgaaga aaaaaatgcg gacgttatca cattctaaat
     5101 gatccgtgag tttggtcaat tgttcgtcat ttatttgtcg attttcagtg gtcgccagag
     5161 gaaatctcga atcgtttgaa gcatgaacat atggactttt caattagttt tatcacgatc
     5221 taacgtgcaa tttgcaatga tcttttcgat acagaacccc tctcacacgg caataaaggc
     5281 ctgatccgtt cattgcacca tcgaggaaaa acaagacata caaaaaatca tgtcgagtct
     5341 cgtggaaaga tttgccttac tcattcgatc catggccgtc caaaatccgc agatgataga
     5401 agtgattttg gtcattggaa agccgatatt gtagctggca aaactggtgg tacttgttta
     5461 gtgactttga cagacagaaa atcatgcttc cttttagcag gaaagaaggt ccccagaaag
     5521 aaagccgctt atgtgagcga gaaaatgatt gaacttttaa gcagtattcc aaatgaggcg
     5581 gtcaaaacga tcactccaga tagaggaaaa gaattctcca atcatttcgc agttacagaa
     5641 gcattgaata atatgccctt ttattttcca gatcctcatg cccttggcaa caatgaacaa
     5701 attaaaatac gaatagactc attcgagaat acttgccaaa atcgaaagag atagctacgg
     5761 tagataactc aacgataaat cagatgattg aaaaactgaa taacagacct agaaagtgtt
     5821 tagattggag aacaccttat gaagtcttct ttgataaagt gttgcgcttg atttgacaat
     5881 tcaagacata ataaaaagca gatcaaatcc aaataaggga aaaatgatct actctggtat
     5941 tatcaattaa ttggccaagc tattatgccg ttttgctaaa cgtctacttc ttccagtatt
     6001 gaccttgata aaacaacaat caggatcccg ttttaatact ctatgaactg tgtcaatact
     6061 aacatgtaaa gttgcagcaa tatcagctaa tgtactccca tttaatctca tttgctttgc
     6121 aaatcgataa cgccgataag catcaatatt cttggaatcc atagacaata atacttttct
     6181 taccatgtat ttcctccaaa aattgaattt taatgagcca tcatttcttg agcaatttca
     6241 tgtttagaca atgaagctgg atagaaagta ggccaattat gcaattcatt atagagtttt
     6301 tcttgacttt ctccaccaaa gaaaatatgg aaatgctctg ccttacttgg tgcaattcca
     6361 tgatcactaa attgaatata tttaaaacga ccagcacctg catcatcagt ttcaaaatta
     6421 aaacgaacac cacggtttcc tttttcataa ttcaaaattt tgtatccaac atatttgtat
     6481 gtgtattgat gatgttcacc gttgactaca aaatcaatgg tgttgtcttt aatgttgata
     6541 gtatcaatat ctgtcttata gccagtagta taataatctt tgtactcggc agctgtcatg
     6601 tctttattga tttttgcttt gtaatcgaaa acttcatcca aaattccatc ttttaacaac
     6661 ggataaacag attgccattc gcctgtataa tcactcaatt cgcgatcctt cacatcggca
     6721 tctttaaagt acccattagc aactgtcttt tcatccttcg cttctttttc tggaactacc
     6781 tcttcaccag caaccatagt tgttttttct aacgctttta gattgtcttc cattactgaa
     6841 acataatctt caccattatc catttgttct tttgtaagac tttcaagagg attcagtact
     6901 tccaatttta tcccagcttc gttggccaaa gtttttgcaa ttttatcatt agcattcttt
     6961 tcaaagtaaa tataattgat cttatttttt ttaacatact cttttaattc agctaatcga
     7021 ccagaagatg gctcttcttc aggacttagt ccagcaatag gaacttgttt caatccataa
     7081 tccaaagcta aatatccaaa ggctgcgtgt tgcgtcacaa aacttttttg tttcgcttct
     7141 ttaagactag tggtatagtc agcatccaac cgtttcagtt tagtcaaata cttttcagca
     7201 tttgtctcaa atacttttgc ttttttcgga tacaatttaa ctaactgatc cttgatattt
     7261 gaaacttcct taattgccat tttgggtgaa acccacgtat gaggatctag ttcatgatgg
     7321 tgatcttctt ctccgtgatc atgatcttct tcttcactac ctggcaataa aatcatgtct
     7381 tttgttcctt ctactacatt aggcccagct ttcttccaac tttctttagc ttttggtacc
     7441 catgattcca tgttttcgtt atgatataca aaaacatcag catcatgagc ttttgccata
     7501 tccttagctg agggctcata gtcatgaggt tctgatcccg ctggaaccat taaatccacg
     7561 ttcgcttcat cgccgacgat gtttttggtg aaatcataca ttggatagaa tgttgttaca
     7621 atttctaatt ttccatcttt atcattcgat acttttgcat cattagtgct ggagcaagct
     7681 cccaataatc ctagagctgc tatcccaata agtcctacac tgattttctt cattcttttt
     7741 ccttccttaa ttatgctaat ttcttcattt tatttcgttt atgttctttg ataattttta
     7801 atgccctttt tctagtttta atattcgggc ttttaagtcg tcgatatgca ctagatatat
     7861 ccgttttctc cattagaaat tacccccaat cactacttat cattatttgc tttccgccag
     7921 taatttatca aaactctgtt tgatcctttc ttcatccaac atttttccga ttagaataat
     7981 agtggtttgg tctttttgtc gagtggtgta ttgaaaatta acctgttcgt ttacctcttg
     8041 catggcaact ataaaatcct gctcttgtaa agcaatcaat cctttaaaac gatacaggct
     8101 cccttggttg gttaaaatca gccagtcaat ccattggagt aaaaaatctt tttttatgtc
     8161 ttgatctgaa gttaagagaa ttgatttaaa ctcatgtact tgatggtgtt gatgatagta
     8221 tttgccatta tagtgatttt ctgatgaatc actttcaaaa actaagggat gattaaattt
     8281 tttcaaatta taaaaatcct ttttttgaat cgtttcacta tgtgtgaaca ttcgaatatc
     8341 ggataaaggg ttaatacttt tgatttctct ctgtaatttt tttagtgcag ataaattcaa
     8401 gttttgtttt ctagacacaa aaatgcgatc tgccatgaca agttgcttaa gggcttgtgg
     8461 ttctctttca ataagttcaa aactgtcact atcaataaca gtcaataaac tatcgatata
     8521 tactcttcct cttagttgag gcgctgttaa aagtgtttga atgattggtt ggggatctgc
     8581 tatacctgta gtttcaatga tgatttgtga aattggttgt tgatgttctt ctattgtttc
     8641 cagtaaagaa atcaatattg ctaataaatc actgcgaaga ctgcaacaca tacatccatt
     8701 atttagttgt aacacccgtt cctcgctatg aactaataat ttgtgatcaa tcccaacttc
     8761 accaaactca ttttcaatta taataatttc ttccctgggg tagggtgaga cttgcaaggc
     8821 ttgattgatt agcgttgttt ttccagcccc taaaaaccca gaaacgattg taactggtat
     8881 gcccattttg tcaccttttt accacttagc tatttcactt ctttaaaaat tgcccgtctg
     8941 cgcaattttg gcgaatactt ttttaattct aaccgttcag ggttattccg tttatttttg
     9001 ctggtgagat acagacgttc tccagtttcg acacattcca aaataatatt ttgacgcata
     9061 aatcttcctc cttcaatact tatcgatctt caagatatgc ttgaatttgt tgtgcatctt
     9121 caaaagttaa ctcgccagat tccaattgtg aaactaaatt ttctttcata tcaacaggta
     9181 tctcccaata accatcgata cattgaaata actcctcgtc atactcttca aaaattggat
     9241 agattcttaa aaaatcatca aatgataagc cttggtcctc atagtaatct ttgactaatc
     9301 cattttcaat tgcctgttgg gtcggtaaac ccgtttcagt aataattcct ctctctacca
     9361 tcgaacgata aaaagtgcat ttatctagcg attgctgggt cgtttcaaga taatacttta
     9421 atccttcttc aactaaaggt gggtaaataa tttccatttt gattccctcc taaaagttat
     9481 atattaaact attttgaagc tcctttaatt actttctttc cattatagta cccttttaaa
     9541 gatacatgat ggctacgtct ataatcacca ttcgcttcgt caaatgaaat ttctggtttt
     9601 actacagttt gatgagttct tcttaaccgt ttcttcgcct tagatgtttt tcttgctggt
     9661 actgccatga tatttcctcc ttttaccaac ttgctttttt tactccagga atcaaccctt
     9721 gatgggcaag ttctctaaac ttaatacggg acataccgaa ctttcgcata taccctcttg
     9781 gacgaccatc cgtctgatca cgaagcctca gacgattcgg atttgaatct cttggcaatt
     9841 tagcgagagc ggtgcgatct cctgcggctt ttaattcctg acgtttatct gcatactttt
     9901 caatcatcgc catttgtttc tttgctttag caattttcga cttttttgcc atataccttt
     9961 atactccttt tattttgatt tagacacctc aaatcgtaat cgttacgttt tgacactgat
    10021 actttaccaa caagagttgc tcttgtcaac aataaataac atgaaataaa actcatatga
    10081 atagataact atttgaaaac gaatttatcg gtacatagag ataatcaaat catttaaaag
    10141 aatatgcagt ttaggctctg aaaaataaga attcactttt attagtccaa tcaaacacaa
    10201 ataccgtttt aagtagcttt tcctctactt aaaacggcaa tatgaaagaa actataactt
    10261 tttattcaat cttttatgtt taactggttt atcgccaatt ttttaaaact ttgattattc
    10321 cttatattat aagaatagac attaagtgaa ctaattcaat catactgatt ctttcgtgta
    10381 ttcaaaatta gatgctgttt aataaaatta ttctttttga aaaagaatta taactatatc
    10441 ccaaaaattg taggttaatt taaagcctat ttcatcatcg tgattgagct tctttttcat
    10501 agacgaattt ctcagatagt ttagttttct ttatgagatt tttgtatatt tctgaccttc
    10561 gatacaactc attatgagta ccttgatcaa ctagttttcc ttgatccatc actacaattt
    10621 tatttacctt ttcaatagac ttcaagcgat gcgatataat tagtactgtc ttatcttgta
    10681 ttaaacgatt taaactttcc tgaatacgtt tttcattttc cgcatctaaa ctggcagtaa
    10741 tttcatctaa aattaaaatt ggcgcatttt tcaaaaaagc ccgtgctatg gataggcgtt
    10801 gtcgctctcc tccagaaagt gaacttccgt tttctccgac caatgtatga taaccgtcag
    10861 gtagtttttc aataaagtct tcgcagttag ctaaacgagc tgcttttttc acttcctcat
    10921 ctgacgcttg actatttcca atacgtatat tttccaaaat acttgtatta aacaaagtta
    10981 cctcttgaaa aacaatagcg attttgctaa acaaagaagc tggagataca cgtttaatat
    11041 catacccatc aattaaaata cagccttcat cataatcata cagtctagac actaatttca
    11101 agatactagt tttcccggaa ccactagcac ctactaacgc agtcacttct ccttgtttag
    11161 ccgtaaaaga gatatgatct aaaattgggg tattgttatc atatgagaaa gagacatctc
    11221 gcaactcaat gtcaaaagat ttcagaggac tatctgatcc ctcttggata ctggtctcct
    11281 tcatagctct aattcgctgt attttaggag ctaaataaaa tatttcaagc actgcttctt
    11341 tcatagaatc gaaggaatcc tttattttta ttgcggctag taagtaacca acgacgtaga
    11401 gtatagtcac ttcacccgtt aacagtaagt gaacacctac tagaatgact actgctaaac
    11461 taacatatga gaaaatagag gataaggcca tgactgaaaa agtacttaat tctaccttta
    11521 aatgaatcct ttcactctct tccatttttt tgaaaagtct gtcttgaact tttttagata
    11581 gattaaagct attaatctct tgctgcaatt ctatagtttc ttgaaattct tccgagtttt
    11641 ctctcaaagt atcgtaatat ttagtattgg ctttcgtttg ggatttcttt gataacaaga
    11701 ttaagacaaa actaaataac gttggcaaaa taacagctaa tcccattttg acattaccaa
    11761 ccaaaagcat cactgaaata aaagggaaaa acagagccat accgccggct ttaggtattg
    11821 catgactcat cgcatgctca ataccttcaa cgtcagacat gatagtttga gataaatctg
    11881 ataaattatg ttttgaaaaa taggataatg gtaaatttga caaatcgtct gcaatttgca
    11941 ctcttaaatg cgcactttct ttataagtac tattatataa gttttcatac tccctatcta
    12001 acaaccaata catgaaaagc aaagtaacaa acgatacggc aaaataaagc caacggggct
    12061 ttgtattttc taaaactaat tcgtctatca gcatcattat aatgaatgct ggtaccatat
    12121 taatgcagta tacaaaaaaa ctagaaatag aagctttagt taaagcttct gcgccctgat
    12181 cacttaaagc aaatttgttt ttataaaaac ttttcattgt caagcctcca atcatttgca
    12241 ttctcataat tagtgaccaa ttgtttatac acttgatgtt tagccattaa ttcattatga
    12301 tttccacgtt ctactatttt cccttctgac aagacaagaa tttcatctac agatttaatg
    12361 ctggataatc gatgagctat cataataact gttttttctt ttatcagatg ttgaaaagcc
    12421 ttttgtagtt catactcatt atctggatca atagatgcac tggcttcatc cataataaca
    12481 atctttgcat cttttaaaat tgctctggca atagctatac gttgtttttc tcctcctgac
    12541 agataaactc ctttactacc aattaaggta tgctctcttt cgggaaattt ttctaaaatc
    12601 gattcgcaac cggctaatct tagcgcgtca aatactttac ttgctggcgc ttgaggattt
    12661 gctatagaaa cgttatcgta aatactctga ttgaatagtt tcgtatcttg aaagacaaaa
    12721 gcaattgtgt taataattgt gtcttttgaa tagtgattta aaggcttacc accaatttta
    12781 atctgtccct tatccacatt gtagaaacct gaaattagtt tagctatcgt actctttccg
    12841 cttcctgatt ttcctactaa tgcataaatt cttttttgtt ctaaaactaa gttgaaattt
    12901 tctaaaacac actcttcttt gtatgaaaag ctgacatttt caaatgcaat gtcacaattt
    12961 ttaatctgtg tctcgttccc atattttagt tggtcttgct tcatttctaa ataaagggag
    13021 tctagtgttt cgactgcata tcttgcttca aacaagtgca tacttacata cattattctc
    13081 atgaaagaaa caaaaataat tccacttaaa aaaaacaaca tcaacaagtc aacagctaat
    13141 atcttgggag tggtaagaga agttataaaa aaggttatag gaactatcaa aatgcaaact
    13201 atgccaaaaa atagccattg aaaaagaaca aaaggtgttt tcccactcaa agaatactta
    13261 tatgcaaact tagcataatc gttaattgct ttattcaagg ctttaaatga tgttacttta
    13321 gcaccaaata tctttataac ttgaattccg cggatatatt ctaccgtttc agagcttaac
    13381 acatctaacg attcttgata tttttgaata aaatttgtat tctctgtcat tttctttaac
    13441 aagaatacac caattactaa catggcgatt agaaccaatc caattttaaa gttaatgaaa
    13501 aagcctaagg caactgataa aacaggtgtt actatggctt gagcactatc tggaatcaag
    13561 tgagcaattg ccatatgtgt tttagccgcg ttgtcatcaa ttgtctttcg aatatatccc
    13621 gaagaattta aatcaaaaaa acgaaaactt gcttctgtta aaccgctaat tccctttttt
    13681 ctcaaattag tctctaagcg aaacccaagg atatgagaaa ataagccaga aaaaatataa
    13741 aataaagctc ctaaagttaa ccaagcaaca gtttgaaccg cataactctt agctaaatat
    13801 tcatctccta caataattag agactgaaga aatttgtaaa tactgtaata tccatagact
    13861 gttaatacga cagaaaaatc agaaaataaa gtggctaacc atccgtagta tttcatttta
    13921 gggacataac taaataattt tttgtatact ttcatttttt cctcccccgc attcaatttc
    13981 ttctcttatt cgtttacaac tgttaatgct ttccgtaata gcgtaaatta gagatgtaca
    14041 aacagataaa aggtaaacta cgttctattt tttgtatttt tttaagactt ttcttccaat
    14101 caatacacca atcatagata aacttgctac aactaacact gaaatgattc cccatatacc
    14161 taccgcatag tggatcattg catccattgt tgcagcagat tgtcctttat ctatccaatc
    14221 ttgacgaaaa cgatctgtaa agaaccatat aggaatccac tgtccggcag cactgcctaa
    14281 ttgcattaga gcccaagacg caacttgagg aattgctcga tcatatccaa atacagatag
    14341 aataatatca gctaagatcg ccatactaat cgaactgata aaccaaggta agtacgcccc
    14401 gcccattaac gtaaaaaata acaataaaac aattgtgtaa atagtaaaaa ttccttttct
    14461 agggtagtta tagctaatat aaacaaatat gattccagat aataatccaa aaatcccggg
    14521 ggatatgctg tgaccaaacg ccccaccaat actactaacc attattgaaa aaaagtaaat
    14581 tactagggaa ataactgtag ctaaaccaat aaaa
//
LOCUS       DACDIE010000059        14482 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.059, whole genome shotgun sequence.
ACCESSION   DACDIE010000059 DACDIE010000000
VERSION     DACDIE010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.059"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(287..649)
                     /locus_tag="IV250_002158"
     CDS             complement(287..649)
                     /locus_tag="IV250_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769411.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(723..1103)
                     /locus_tag="IV250_002159"
     CDS             complement(723..1103)
                     /locus_tag="IV250_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAP8769412.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1185..2360
                     /locus_tag="IV250_002160"
     CDS             1185..2360
                     /locus_tag="IV250_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP8769413.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2436..3020)
                     /locus_tag="IV250_002161"
     CDS             complement(2436..3020)
                     /locus_tag="IV250_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP8769414.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3221..4219
                     /locus_tag="IV250_002162"
     CDS             3221..4219
                     /locus_tag="IV250_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP8769415.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4331..4840
                     /locus_tag="IV250_002163"
     CDS             4331..4840
                     /locus_tag="IV250_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAP8769416.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4870..5529)
                     /locus_tag="IV250_002164"
     CDS             complement(4870..5529)
                     /locus_tag="IV250_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAP8769417.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5531..6295)
                     /locus_tag="IV250_002165"
     CDS             complement(5531..6295)
                     /locus_tag="IV250_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HAP8769418.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6303..6980)
                     /locus_tag="IV250_002166"
     CDS             complement(6303..6980)
                     /locus_tag="IV250_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HAP8769419.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7017..8402)
                     /locus_tag="IV250_002167"
     CDS             complement(7017..8402)
                     /locus_tag="IV250_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAP8769420.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8612..8696)
                     /gene="ffs"
                     /locus_tag="IV250_002168"
     ncRNA           complement(8612..8696)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="IV250_002168"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8719..9234)
                     /gene="tadA"
                     /locus_tag="IV250_002169"
     CDS             complement(8719..9234)
                     /gene="tadA"
                     /locus_tag="IV250_002169"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAP8769421.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9341..10198
                     /locus_tag="IV250_002170"
     CDS             9341..10198
                     /locus_tag="IV250_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAP8769422.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDGTEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10279..10614)
                     /locus_tag="IV250_002171"
     CDS             complement(10279..10614)
                     /locus_tag="IV250_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769423.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10681..12051)
                     /locus_tag="IV250_002172"
     CDS             complement(10681..12051)
                     /locus_tag="IV250_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HAP8769424.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12129..12665)
                     /locus_tag="IV250_002173"
     CDS             complement(12129..12665)
                     /locus_tag="IV250_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8769425.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            12974..14455
                     /locus_tag="IV250_002174"
     CDS             12974..14455
                     /locus_tag="IV250_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP8769426.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         4461 a         2882 c         2354 g         4785 t
ORIGIN      
        1 ttttttaaat tttttagaaa tgtgatataa catccacttc agaatcaacg ctgttaaatt
       61 tatcatgtat ttcttccttg gtcaactata aattaagcat tactttgtga aatgattttt
      121 cgatcaatta ttattattcg ttatacaacc gttttcaaca tattttgaaa ataaaatgca
      181 ctaaaaaact atctttagca gaacatttca ttgtattttt tggaccaaac aataaaaaaa
      241 gaagtttgaa aaaaattcaa acttctttgt tcgcttaatc tgtattttag aagaaaggca
      301 ctccctctac ttcgtaagtt ccaagaacag agcgcatttg acctggttct tcccaaatat
      361 atccttcgcc taattcttct ctttgattct tttgttcagt ttcaaagaca aacaacggaa
      421 cattttcctc tttaatatgt gtatttgtca attccatcaa actaatcttg ctaacatcga
      481 tatgaactat ttcttttaag aaattaagta tattcgctaa accagtcttt ccttcagaaa
      541 gttccgctaa cgcaaattcc aacttgtcat caattgaatg gaccaaaaat tttttagtac
      601 catcctccaa atgcaacata attgtaccag caactctttt gcttgccaaa atttccacct
      661 cgatcaactt gttctatggc ttacataaaa attctagcac attttcagaa aaaatacact
      721 ttttatttct tattttcttt ccgttctagt tcagataatg cttcttctat ccaaataggt
      781 aactgctccg caatagtggt aaatccgatc ttacgatttt tattcgtgaa ataccttttt
      841 ctgcgatatg gaacagctgg atttgttttt tctaacgttc ttaaagataa actgattttt
      901 ttggaatatt catcaatgtc gatgatctga actgttactg gctgtccgac ttttaacaat
      961 gaatggatat ttttcgtata accggattgt acttctgaaa catgaataag tccttgcgtc
     1021 tcgttatcaa gagaaacgaa tgcgccataa ggctgtatcc ctgtcacttt tccttcaatt
     1081 atttgtccaa ttttataagt catctacttc cctctcttcg catttatcat tatactgtaa
     1141 atagctcaaa aaaagaagcg acgaaaatga ctggaggtct taagatgatc gaatttgata
     1201 aacagatttc aagaaaagat acagaaagtg taaaatggga cgcaattgct gagacatatc
     1261 aaatggatga cttacttcct ttgtgggtag cagatatgga cttcttgtct cccggtgggg
     1321 tagctaaagc gtttagtgaa tacatcaaac atggaatctt tggttatgcc actctgtctg
     1381 ataaactata tcaggctatc attcattggg aacgccaaca ccatgctgtg gagttgacga
     1441 aagaaaatat cgtttttaca agtggtgtgt taaccagttt agctgcagca gtacaaactt
     1501 ttacaaaacc aggcgattct attttgattc atgacccagt ttatcctcca ttttcctcta
     1561 ttatagaaac aaatcaacgt cacatcgttc gaagctcttt gctagaaaaa aataatcatt
     1621 tcatcatgga cttgtctgac atggagaaaa aaatcgaaga aaacaatgta aaagcaatga
     1681 ttttatgcaa tcctcacaat cctggagggc gtgtttggag caaagaagaa ttgaaacagt
     1741 taagcgaatt gtgtttaaaa catcaagtga ttctgtttag tgacgagatc catcaagatt
     1801 taactttatt cgatcataca tttacttcca tgctaacgat tgatcctaat ttagatgatc
     1861 tactagttgc ttttacttcc gcaaccaaga ctttcaatct agctgcaatc aaaaattcaa
     1921 tggtatttat caaaaatcca gagatgaaag ctgattttga aaaacattta gtgatgaacc
     1981 aacagcacga aatcaacact tttggactga ttggaaccca agcggcttat gagacgggtg
     2041 gagaatggtt aagccaactg attccttatc tagaaaaaaa tgttcaaaca gttaatcagt
     2101 tttttgaaca gtatcttccg aaagtgagaa taatgaaccc tgaaggaact tacttaatgt
     2161 ggttagattt taccgcctat atttcagacg accaagaatt ggagaacact cttgttcgga
     2221 aaggaaaagt cgtattaaat cctggcatca cgtttggtcc ttccggtcat gggcatatgc
     2281 gcttgaatat tgcttgtcca gaagaaactt tactggaagg acttaatcga atcaagtatg
     2341 ctttagagac aatcgattaa atatttattc aaaataaaag gacgagacct tactaggttt
     2401 cgtccttttt cataattact ccgtaccata ccaaactatt ctgaaatttc aattgtttca
     2461 atcactacat catgtacagg tcggtcttgt ggaccacgtt gtacatttgc gatttcatct
     2521 actgtgtcca tcccttctag tacatgtcca aagactgtat gtcggaaatc tagccaagga
     2581 gttccgccac ctttatacgc ttcaatgatt tctgatggga atccagcacc ttctagttgt
     2641 ccaagcatat tagctggcac atttgtgtta tttacaataa agaattggct tccgtttgtg
     2701 tttggacctg aatttgccat agataaagct ccgcgaaggt tgaacagttc acgactgaat
     2761 tcatcttcaa aactttcgcc ataaatactt tcgccaccca ttcctgttcc agttggatcg
     2821 ccgccttgga tcatgaaatc aggaataacg cggtgaaaaa tcacgccatc ataataacct
     2881 tttttggaaa gttcaatgaa attttgaacc gtcttcggtg ctagttcagg gaataattga
     2941 acagtaatgt caccacggtt tgttttgata acagcttttg gtcctttttc actttttaaa
     3001 tctagttgtg ggaatgccat attgtttcct cctaatattt gcatacttca taataataat
     3061 aacataatac ctaacctctc gtaaaaatac tagatttgtt ttcttttatt tttcatcact
     3121 agatacattc tataacttac cgtttcgatt tcgaattcaa acatttttga gaagcattct
     3181 caattaaatt ctgctatagt gtgaaaaggg aggaattttt atggaaatct atgacattac
     3241 aatcatcggt gcaggtcctg tagggatgtt tgccgctttt tacgcaggaa tgcgccaagc
     3301 taaaacaaaa atcattgaca gtctacctca acttggtggt caattagcaa ctttgtatcc
     3361 cgaaaaatat atttacgata ttcctgggta tccagcaata aaagcaagtg aattaatcga
     3421 tcaattagaa aaacaattaa ctacttttaa ccatacattc catttaaaag aagaagttct
     3481 ctcgcttact cgtgaagacg aagttatcga gattactaca aataaaggca tccattattc
     3541 caaagcagtc attttagctt taggaaatgg ttcattccag ccaagaaaat tgaatttaga
     3601 taatgctgaa tcatttgaaa accacggatt ggattatttc gtgaatgatt tgatgaagta
     3661 tgctggaaaa aaagtcgcta ttgctggagg tggtgattct gctatcgatt ggacgttgat
     3721 gttagagcct attgcaagcg aagtttactt gattcatcgt cgcccggaat ttcgtgcgca
     3781 cgaacatagt gtatcacgct tgaaatcttc atctgtcaac ttgcttactc cctatctcat
     3841 cgatggatta tcaggcaata atggggaact gacagatatc cggttaaaaa aagtgaaaag
     3901 tgacgagacg attgatttga tgattgattc tctgatcgtc aattatggat tctcttctaa
     3961 tttagaacat ctttcttctt ggggacttga tagtaccaga aatgccatta ctgtaaaatc
     4021 agatatgtct acttctattc caggcgttta tgcagctggt gacatctgta cctatgaagg
     4081 aaaagtgaaa ctgattgcta ctgggctcgg tgaagcacct actgcagtga acaacgcttt
     4141 acactatatt aatccaaagg aacgaactca gcctggtcac agcacaagct tatacgataa
     4201 aaatcttcgt cctagttgat tttttatatc tctttgcatt tcaagttaaa cccgaaagca
     4261 gtttcttgca tttttttcat gttccgttaa catgaaatag agaaagaaaa aataaagggg
     4321 gagcaataat atggtcgtca aaactgagac tttagaagaa aaggcaagag aactattgaa
     4381 ggaacgtggc gtaactgtag aagatatcgc acaacttgtc atgtttttac aaaaagacta
     4441 tatttctgat ttgactttgg cggattgtac tgaaagtgtc aacagcgtgc taacaaagcg
     4501 tgaagtacat aacgccatta tcactggaat ccaattagat atattagcag aaaagaaaaa
     4561 attgatgagt ccgttgcaac atattatcga agatgacgaa ggcctttacg gtatcgatga
     4621 gatcctagcc ttgtcaatcg tgaatgttta cggtacgatc ggctttacga attatggtta
     4681 tatcgacaag gtgaaacctg gtattttaaa agaactaaac agccacgatg gcgttcatgt
     4741 ccataccttc ttagatgata tcgtcggagc gatcgccgct gctgctgcca gtcgattagc
     4801 acacaaagaa ccagaaaaaa gaagtcgcct tactcaataa ttcaacaaaa aaagaaagaa
     4861 aacgtctatt tagacgagtt ctttcttttt tgtttgtttc aagctatttt ttcctaagaa
     4921 aagaaaaaca gcaaaaaata attctaataa tacaaaaaat aagataaagc tatgcatgaa
     4981 aaattcttct ggaagtacat tgatgattgg atcagtaacg ggatcgaata accagtcatc
     5041 gttgttgaaa aacgtttcat gaaaaaggat aaaaaagcga tcaaatccta tcatcatgaa
     5101 aaaaccgaaa acaactggca gtaacatgcc gatttgaaac ggacgaatca accgccaaag
     5161 ccgatcattc ttttgcaaat atttgataaa caaaatactc gggatcaaag tgatgaaaaa
     5221 cacagcataa tccactaaaa acaaaatttt cacttcataa aagtgatggg ctccgctagc
     5281 agaaactggg aaatctggca agctgaggat ggttttgaag ggattattga gataattcat
     5341 taggtgatca aaatttttca gtaacgtctc ttgatctaaa gaagtataat ccaaaatttg
     5401 caaatgtccg atatcaaata cgtatagtgg tcgaaagttg atcgtcacac caactgccag
     5461 actgatcagt gtcagaaata ggctgaacaa tccagcatat tccatccact gccatctcga
     5521 atttttcata ttagaagctc cattcatcca gtgattgttt cacataggta gctgggatag
     5581 gcaacccagg tacatcttct tcttttgtaa aacctgataa aacgagcaaa gtatctatat
     5641 tattgcgtat cccagcttgg atatctgttt catagttatc tccaaccatg attacttctt
     5701 ctttttgcag ccctaatact tcaacggctt tatccataat gatggcttct ggttttccga
     5761 tgtagatcgg cggcgtttgg gttgctgtct ctacaaaaga gatgactgac cctgctccag
     5821 gaagaaggcc gcgttcagta ggtatattct tatctgggtt cgttccaatg aaagtagcac
     5881 ctttttgaat agcaagtgtt gctttaacta ctttttcata ggtcagataa ttatctaagc
     5941 ctaccactac atagtcagga gcctcttctt cccatgtaaa tccggcagct aaaatcaagt
     6001 caatcaatcc tgcttctccg ataacataga cttttttccc ttttccatct gctttcataa
     6061 aatcgattgt tgccaaagta gctgtgtaga ctgtctctgg agctacatga atgtcaaact
     6121 catctgctag acgattagct actgtttctg gtgacttagt cgtgttattg gtaacaaaca
     6181 agaaaggtaa ttttcttttt tgcaattctt ccacaaatcg tttaccggcc ggtatgggtt
     6241 gtgtcccacg ataaatcgtt ccgtctaaat caattaaata tcctttatag tccattcatc
     6301 cactactctt ccctttttct gatcgtaaat gctgtttttg ttttttctac ttgtttaggt
     6361 tgttcttgtt tttttacttt ttcagaggtt tttgctgact ctgtttttcg atttttaata
     6421 accggttttt tgcgattaga tgcgactggt ccttttttct catcgatata cgcacgatta
     6481 tggttttttt tcttttttcg gcgctgagtt gtcttttctt ttttaccgcc gattctctcg
     6541 atgacaaaat acgcacaacc aaaattgcaa tattcgtaaa gatagtcttc caaggaatcg
     6601 attcgctgat ctggatgcgc ccgtcgatta tccgcttgga aaaatccttt taggcgtagt
     6661 tgttcataac cccaatctcc tacgatataa tcatatcggg ctagtacatc actgtaacgt
     6721 tctccaagct tttctgcatt aaatccttca cgataatcgg acacaagccg ataatgtctt
     6781 tcacctatca aaaaattact gtcatctaac agtgtcacta gctcgccttt gactttttct
     6841 gctttaggct cctcaacgat ttcttccaaa gcagctgtca gttcttcggt gactgttgtt
     6901 tcctgttcag ctgatgattt tttcttagca atttctgttt tatgctcaac gttctgctgt
     6961 tcattttgtt ttgcagacat gcgttcacca cctatctttt ttattatacc ttgtttttat
     7021 ttgattgggt agtgagcttt caagtaatct cctaccacac ttcggataaa ttcagggaac
     7081 aaaaattcat tttttccagc aatctcaatc gttggaaaaa atggaacaaa catcaaatga
     7141 tctacggtag taaaagtata atttttattc agatcgattg gcttgttctt ccaaaagact
     7201 tgatggttaa tagaatcata tgtgattcca ttatagacca tctgcccaaa aatcttaccg
     7261 cgaaatccca ttcccttcat aggatagtta cgtaaaaagt tacgattttt ttctatttct
     7321 aaaactaagc ggatcagatc atttccaccc aatgtgacat ttaacaagtg catcggatgc
     7381 ggcaacgcag tgtgaagctg atcctgattg acccgccctt ttggaatttc agttaaaaac
     7441 agaccactgt ttaatattgc tgcttccgtc tttcctcgtt ctttcaccgc ctcaaaggct
     7501 acttggataa acgaatgctc attgaagata tctagactca gagaaaatgg caaatctgct
     7561 acttcttttt cttttaataa ttcatgccca cgttctagat aactatttat ttcttgttca
     7621 tcttctgtaa aagctgtcat tgtttcggta gggatggctt tggctgaatg cttaaggatt
     7681 tttttctctt catcgatggt taaatggact tcaccgacat actgcccgta ctttccagcg
     7741 gctgctattt ggacatcatt gattacgtga ccttctctga ataaatgatg ggtgtgcgat
     7801 cctaaaataa catcaattgc tggtatttct tttgcaattt ggaaatcatc ctgaatcccg
     7861 agatgactca tcaaaactaa aacatccact tgtgaacgta aatcttcgac taattctggc
     7921 aaaatatcat aaggattccg tatgtcccag ccattcggat tataagtaag cggaaatgga
     7981 gcagtaaatg caattagtcc gattttcgtc tggtgtttcg ttgtaatgat tttatatggt
     8041 ttcgtccatg ccggtggctg gagcgtattt ttatcgaata agttatccag gatgatatca
     8101 aactcagcat gatcgtataa atgattcaac tcttctttgg aatttcctac gccttcattg
     8161 tttcctatcg tcgctgcatc ataaccaact tcattcatca gcaagatatt ttcctgtcca
     8221 ttcgttgctt ctgacaatgg atgccaacga tccacaaaat cacctagatc tactgcaatc
     8281 gttgtcgttt tttctttctt ttcgttttct cgtttacgtt gatccaaaaa ccttctgatc
     8341 cttggccaat tttctaaatg agaatgcaga tcattcgtat gtaaaatcac tattttttcc
     8401 atgtaccctc tcgcctctct ttatctttat cataccatag gcagcttttt cttccattga
     8461 aacaagctct atttcagagt ttttcctgcc ttgaagcaaa ttgtataagt acaatattcg
     8521 ataaatcagt ctggctaaaa aatcaggaac attcattttt acagtactat aaaaataaaa
     8581 aaaacagacc cgaaagtctg ttgtcgtttc attataatca gacacatggc ggaacctgct
     8641 tagtgctgct tccttccaga cctgacacgc ttcacaagcc caccattgtc ctagcctttc
     8701 ggcaaagaat agtatacctt atttttcatt cgttgaccag tcttttttta atttcttttt
     8761 tcgctggcgg agctttcgga aaaagtcagt caaaatcatc ccacattcgt cttctaaaat
     8821 gccaccttct acatatgctt ggtggttaaa ccgctcgtcc tccaaaagat tcatcaaggt
     8881 accggctgtt ccgccttttg gatcatacgc gccgaaataa acttctgcta cacgagacaa
     8941 aatcatcgcg ccgctgcaca taggacaagg ctctaaggta acaaaaagtg cagcatcttc
     9001 caagcgccag cttcccactt tttcacaggc ttccttgatt gcaagcattt ctgcatgtgt
     9061 agtagcatct tgcgtggttt ctctaaggtt atatcctctt ccaatcactt ttccatccaa
     9121 tacaaccact gctccaatcg gaacttcatg aagcatttct gctttctttg cttctgccaa
     9181 tgcaaggcgc atccattttt ctttttctac tgcatcaaga tttgtattta ccaattaccc
     9241 ttccctgcct ttgttttttc ttgttttact ttagcatatg ataaaaaggc tgtaactaac
     9301 ttttttttct atggtaaaat aaatacaaga ggtgaacaag atgaaaatgg ataaattact
     9361 tgctctttac ccacaagcac agaaaaaaaa ctctccagca tcagaagcgg atatcctttc
     9421 ttttgcaatt gatgatgctt ttatctggat caaacaagat tcattatctc aacaagaaac
     9481 cagccttttg aaagcgttat tccctgtaat caacgatcaa aaaaaacacc catggtatcg
     9541 ctatcttttt aaagatgctt cttgtgttca agaaaaaagc tttcgtttga tccagcttca
     9601 cgtcgaatca aaaggagaat tcttaaaaaa agaatggcaa gatactattt tggaattatt
     9661 tcgctattct gctgatttct ttttttatac agacacagat gcgctcttag tcgaacaaat
     9721 aaaccgtgat tatcttgatg gtactgaaat caacggtatt tttctatctt tagatgctga
     9781 ttttgatacg acaacgtctg tattcgttgg ttctttccat tcacctggca ccgacctcgt
     9841 ccctttgttt gctgaagaaa gaaaaatttt tttaacagag caaaacaatc tttatactca
     9901 cagccgtact ttttctatgc aagatgtggc tttacattac tatacaaaag atactgtaaa
     9961 agatagtaag ttgatcaaaa gttacataga actgatcaaa caaaatgaaa tggaagtgat
    10021 cattcaagaa ttatggaatg aattaggcaa tgtcagctct gctgcaaaaa cactgtttct
    10081 tcatcgtaat actctaaaat atcgaatcga aaaatttcaa gaacagtctg gtttcaatct
    10141 aaaaaaagcc aacgatttac tgttttgcta tcttttattg cttcatgaac ataattaaag
    10201 atagattaaa ggggttgtga caataatttt gtcacaaccc cttatttcga atgaatagtg
    10261 ttcaaaagct agtttctctt acatcatttt ttcaacaaaa tgcattagcc ggtcttttac
    10321 atcttcggtt gaatctttga ttgaatcacc ataaacagag attttctttt ttcctgattt
    10381 gatttttgct aacattccca tcaaagaatc caagtcacta tctgaaagac catctacgat
    10441 ttctagtaat ttttcatttc catcaaattt gtcatagacg aattttttta ctttcgtccg
    10501 agtagtcata tgatggatct tttccattac ttttcccgat acaacgactg ctgtcgctac
    10561 actggcagcg gctgctacgc ttaatccgat tgttactttt gttgatgttt tcataagtat
    10621 cagctccttt ttatctatct ccatcatagc atagacaaaa aaataaacga actaatccac
    10681 tcagtgaaat ctcaatcctt tagggaaaaa gttctttact gttcctttta cttgtttccc
    10741 aaatccgaca ggtatcttat ctaatactag caaaacccat ccttgattcc catcacgttg
    10801 aaatgtttct cctgctgtgt aagattgcca ttccttttga gtgatcggca agcaaggtat
    10861 attctctatc ttttttgtag caagcgctaa tgcatagcta ggttcaaatc gattcttttt
    10921 gaaatctcct aaatgcaaac ctgtacgtac gacttttaaa ccatcgagag aaggggctaa
    10981 ctcaggaact tcccaaagat ggtcgttgaa cactaaaagt cttccagtag cttcataatg
    11041 aaaatcattt gaaaactccg tccagagttt ttcctgctct tttgtcattt gcactttact
    11101 tttttttcgt gtcttctttt ctttatgcat ctgattttgt ccatgaaaag tcagctttgc
    11161 aacaaaatgt ccttcaccct gatctttatg aggccatata cgtattgttt tttccaaacc
    11221 agctacgctt ccccattcag aacgtcctga actaacggat tgagtcaaag gaatctcctc
    11281 aatcgttact ggataatttt ctacaagcca gctaatgatc tcttcatttt cttctggtgc
    11341 aaacgtacaa gtcgaataaa tcagttgtcc tttatttttt agcattttta ttgctgaaga
    11401 caaaatctct tgctgacgtt tttgacagta tagcggagat tcttctgtcc actcttttat
    11461 tgcatttgga tcttttcgga acattccttc tcctgaacaa ggagcatcaa ccacgatccg
    11521 atcaaaaaag cccgaaaaat gagggacaag ttctgcgggt gcatgatttg tcactattgc
    11581 gttcgaaact ccccatcgtt ctatattttc tgataaaatc ttcgcacgtt tcggaaagat
    11641 ctcattcgtc actaataacc ctttcccctt catttgagca gctaattggg ttgatttacc
    11701 accgggagcc gcacacaaat ccaaaacttt ttcaccgggt tttgctgcag cagctgttcc
    11761 aacgatcatt gcacttggtt cttggctata ttcataccca gcttgatgga ggaaagactt
    11821 tccattgaca gtgccaagaa atccctcatt gctgtaggga gcgggttgta attcttcgct
    11881 atggtaagtt tggaccatgt ccaatcctgc cggttttagt ggattccacc gaaatccttt
    11941 tttcacgctt ccttgttcaa gcgcagaaaa aaaatcggat gcttcttcgc ctaaaagaag
    12001 acgatatttt ttgataaatt gctgtggtaa cgttgtcgct tcctccttca ttccaatgtt
    12061 tcgttagaca ttttacaatc ttttttacga aaaataaagg tggctgatct actttttcat
    12121 gttgctgcct aaggtgactg ttcctttaag atattttcca agtattcatc cgtcacctcg
    12181 tttttatccc aaacagcgtt catcatttca agaaaatctt gagaagaatc atctgagcgc
    12241 aaacttccca atgcattaag ccataccaat tcaagcgact tttcttcttt gatcttcgtg
    12301 tgacctttga catatgagaa aaatggccgc ttaaatagcg aatccatgtc taaatcagat
    12361 tttgcaatga tttcacgaat actttcatgg atagaaaaaa aatctttcaa taagtagaca
    12421 atgcttaaaa catcatcgga actgcattca gctaagtagt tttcaaaata aacagtgatt
    12481 tcttctaata tcttctcttt taacccgctg attgatttga aatgtgtata aatcgggtat
    12541 gtagaacaac cgcagaactc tgctaactct cttgcagtaa ttgacttcat tccatattct
    12601 tccacgtata ccatactttt gaacagcaaa tattctttac tataatttgt tactctcccc
    12661 atcattctta cctccgtctc ttttcatcta cccataaata aataacataa ataattctaa
    12721 aaatattatt gataattatc aaaacatatt tagttatatc cattaaaaat caatttttcc
    12781 gccttaaaaa caagaatatc ttgacaaaat accctaggaa ataaacaata gaataaacaa
    12841 ggtaacaaaa aaattaaata acgttatata aaaaagaagg cgacactctg tgagaataac
    12901 acagactgaa atgactttcc actcacagtt tttgtctaac tatatacaaa aacagaataa
    12961 aaaggaggta tttatgctaa ttgaagcaag aaaaaaagag ttacttgttc tacaaatggt
    13021 cattcaaaaa aataattgtt ctttacaaga attatccaat gacttacata ttccaaaacg
    13081 gacaatcaaa gaactgatcc gaaaaataaa tgtatcactc aagcaatttt tattgattga
    13141 tgaatttatt tattctaatc ataaaggaga gatccaaacc aaagaaaccg atcaaactaa
    13201 aaagttagct gttttttcta agttaaaatt atgctaccta aaagaatcca atcgttttaa
    13261 ctatttgatg ttactaatca attatccaca aactgctgta ccaaaaaaat atttacttga
    13321 acaactttat atatcgccct cctatctcga aaaactgaca aaacagctca atcattcctt
    13381 aaaaaaattc catttacgga tcctatctgt aaaaaattgc tgtactcttg aaggaaatga
    13441 gttatctatc cgtctctatc tttacttttt tctttcagat acatttggag gtaacgattg
    13501 gccattttcc tcacttgaat taaatgcact aaaaatgaac agtacagatg agcaaataga
    13561 aaaaatttca aaacaagaaa aaaatatcta cttgctgtta actttattca tattacgaaa
    13621 caaacaacag gcatattttc caaaaacttc ttctgaaatc actgaattga tggacttatt
    13681 gcagcataca catgattatt cagaatactt tacttctttt tcagtgagtc gcccttgtga
    13741 agagacagaa acagagagag cctactttaa ttattttttc tattgttttt ttccaaatca
    13801 aatctctttt cgacaaaaac aggagattgg ttttttattt tccacaagca aaaaccttat
    13861 ttgtcgacaa gtcggtcaat tgatgactta cttaactcaa cagtttcctc tcaaccattt
    13921 agaaaataaa ataccaattt atttttattt tcttgtgttg gcagttgttt ttttcgaact
    13981 gttgggtgaa tccagcagta gtttttctac actttgcttt ccagaagcca cgtccaatca
    14041 ttctagttct tttatacagg aaaagaaatt aagagaaaaa atcgatacaa ttttttcaaa
    14101 aaatggggaa agaaaccaaa tcagtcaata ccttagtcaa ttgatccaac acttactaca
    14161 aacagaaacg aaagagcagt tactcatcca tttgcagata accaaacagt ttacaggaaa
    14221 ttatcatctt caaagccatt tgcagcagtt atttcgcaaa gatgctgtta aaattaccgc
    14281 atattcagaa catgctgatt tgatcattac agatcagcat gagaaaattt cagatgaaac
    14341 ggagactttc tatctggata caacgcacaa tacagtgtta tttggcaagt taataaaagc
    14401 ggttcatgaa ctgtatatga aaaaaggtta tgagaacaaa acaaagagga tgtgacaaaa
    14461 ttcttgtcac atcctcttat tc
//
LOCUS       DACDIE010000060        14005 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.060, whole genome shotgun sequence.
ACCESSION   DACDIE010000060 DACDIE010000000
VERSION     DACDIE010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14005
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.060"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            3..608
                     /locus_tag="IV250_002175"
     CDS             3..608
                     /locus_tag="IV250_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP8769427.1"
                     /translation="MAKTTTVTTFDNISIKRISFNFKNATNAISTDCNGQLDGETEMQ
                     RIVKKCGSTEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDERIKSGIYSYGPDSVG
                     EDFSLAAEIVDDFEEKNKLIGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFGK
                     FYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            628..990
                     /locus_tag="IV250_002176"
     CDS             628..990
                     /locus_tag="IV250_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769428.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            993..1175
                     /locus_tag="IV250_002177"
     CDS             993..1175
                     /locus_tag="IV250_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769429.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            1192..4611
                     /locus_tag="IV250_002178"
     CDS             1192..4611
                     /locus_tag="IV250_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP8769430.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITVIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQAFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWVSIAWNGIVTTFHTIFDPVVAWWNGIWTAISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWNSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISSIFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGEAGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
     gene            4662..5399
                     /locus_tag="IV250_002179"
     CDS             4662..5399
                     /locus_tag="IV250_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP8769431.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVVGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINYFNCTGLSKIQIYRNGLR"
     gene            5409..7700
                     /locus_tag="IV250_002180"
     CDS             5409..7700
                     /locus_tag="IV250_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769432.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITASQAWQNAKTLAKPVIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLLNDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDNGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            7724..9805
                     /locus_tag="IV250_002181"
     CDS             7724..9805
                     /locus_tag="IV250_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769433.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDNVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAEIKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGGYDYSGNPNLMANINADSFS
                     QGSGALSVVDDGDEVVVTLDPNNKLEIFKAKSQPALVVGKTYTMSVEIMLEGDFTGDP
                     SKIGLRYIKMPNWVSGLYTRNTLTATKGVWQKLTGTVKITAASDNAESWFIMLQNKDA
                     NNSLSGKLRLRHAKLEEGSTATPYQPNLLDAPYYLSKVALGENIADPAVSFPIKTSAY
                     RLYGVNMLEEFKVGQRYTITIKGTKPATQDFWAYNGGNISLERMTPVEGLVDVWTCSF
                     TILKLDSSSPSLLSIYQTPQSTVGSCQIDWLKIEKGDTRTPNIEQYKYRGIGMRDSNN
                     PKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEINSQLTASINEVDTTAKD
                     AQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKLYREGKTVSIYFVALN
                     GKSSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYG
                     SAYSFVITYSI"
     gene            9819..10166
                     /locus_tag="IV250_002182"
     CDS             9819..10166
                     /locus_tag="IV250_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769434.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKSGTAVDDIEDVDQLKQLNALLTKQLAISVQEQEKMQQMLAQLTM
                     EVASIKNGGKSNE"
     gene            10159..10299
                     /locus_tag="IV250_002183"
     CDS             10159..10299
                     /locus_tag="IV250_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAP8769435.1"
                     /translation="MNSFPGFENIKQFYDWGCYTDQDLLDYVNMNCLTKDQYKQITGN
                     EI"
     gene            10399..10788
                     /locus_tag="IV250_002184"
     CDS             10399..10788
                     /locus_tag="IV250_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAP8769436.1"
                     /translation="MTGVIGGTIVGLLGGMDNILHVLIFLVGVDFLTGLAKAWKLKEI
                     SSEIGFEGLLKKVLIFVVIAVAVDVQKIVGNSIPLREIVIMFYVANEGISFLENISVF
                     LPLPDKLKEVFQQIRNDTENKDRGGTK"
     gene            10785..11795
                     /locus_tag="IV250_002185"
     CDS             10785..11795
                     /locus_tag="IV250_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP8769437.1"
                     /translation="MKKKIFVGAIIALFLLPINAFAYSINNEFNLGANEGSSQVANNQ
                     YILLHETANETATGRNEAQYMKRSWYNAYTAYIVGDGGIVYQVGQPGYVQYGAGSYAN
                     ANSPVQIELQHTHDKVTFEKNYKAYVELARDSANKYGIPLTLDTPYNQPGIKSHLWVT
                     QNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTL
                     SDGTNHSHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPD
                     VNQAYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIP
                     KR"
     gene            11985..12058
                     /locus_tag="IV250_002186"
     tRNA            11985..12058
                     /locus_tag="IV250_002186"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12019..12021,aa:Ile,seq:cat)
     gene            12735..13897
                     /locus_tag="IV250_002187"
     CDS             join(12735..13011,13011..13897)
                     /locus_tag="IV250_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP8769438.1"
                     /translation="MSRRQRRTYSKEFKQQIVDLYLAGKPRAEIIREYELTPSSFDKW
                     MKQAQSTGSFKERDNLTPEQAELIALRKKNKQLEMENDILKQAALIFGPKRQVIDANK
                     HKYSISAMCKILNISRQTYYYQAKPIENESDLEEIVQEEFIRNRKAYGTRKLKKCLAK
                     RGLQLSRRRIGRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQEQPLEAIV
                     TDLTYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLTDVKLFH
                     TDRGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQFETLA
                     QLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT         4867 a         2236 c         2946 g         3956 t
ORIGIN      
        1 caatggctaa aacaacaaca gtaacaactt ttgacaatat aagtattaaa agaatttctt
       61 ttaattttaa gaatgcaaca aatgcaattt caactgattg taatggacaa ttagatggcg
      121 aaacagaaat gcagagaatt gtaaaaaaat gcggttcaac agaagtaaaa tcgaaatcta
      181 aaccaatcaa tatgacggta acaattactg cacatgtacc gatggaagtt tatcgacgct
      241 tcaatgggtt gaaacaagat gaacgtatta aatcgggaat ttactcttat ggtcctgatt
      301 ccgtagggga agatttctct cttgctgcag agatcgtgga tgatttcgaa gaaaaaaata
      361 agctgattgg tatgttagca tgcacttcga atacaggatt aacattctct attgaaaatg
      421 gtgcggatga agtagctgcg ttagaactag aaacaaaagt tatgcaagat gaatttggta
      481 aattttatca tgaagcaatt gttgcagaac ttgaagaaga cttaacagat caatggatga
      541 cgaatctatc tgctgatgtg attaaaaaaa cttcagttgt gacaactacg gccactcaat
      601 cacagtaaaa aaaacggagg tagcgaaatg aacgaagatt actcaaaaat tgaactaaac
      661 gatggaacaa ttttgaattt agaacctaaa ctgaatatca agaaattatt gatgatcaat
      721 agagatttta acacagacga gtttgcaaaa atgactgtgg gaaaaggatc catggatatt
      781 tctgttattc aaggtgcaaa ggctgtgtat attgcttacc gccaagcgaa catgactgat
      841 tatatttcat tcgatgaatt tatcgataaa tgggattttg atatggctac tgccagctat
      901 atttatcaat tgatgatgtt caaacaagca cgcgatgctt atcaaaaaga atttgaaaaa
      961 gcaaataagg aaaaaaagct tcaaaagtaa aaatgccaaa gctcttagtt gaaacgtggg
     1021 tcgatgtcta ttcgatgttg accgacgttt tttctatgcc ttcagatttg gttttaagcg
     1081 atatctgttt agatgacatt ttgcaaatgg cttacaacaa gagtgcttat gaaggatgga
     1141 aaaactatgc aataaaccaa tcccagaaaa actaaagaaa ggaggtaaaa aatggctaaa
     1201 aagagaacag aagcagaagt aacattcata gctaacgatg acggattgaa atctacgtta
     1261 aaagaaatca gtgctgaatt aactaaaaat agagcagaat taaaactaga acaagctcaa
     1321 ttacaacaga ctggttctga atcagacaag ttaggaagta aattatcttc tttagaaaag
     1381 cagtatgaat tacaaagtca aaaagttgaa gtaactagcc aacgtttagc caatgccaaa
     1441 aaatattatg gagaaaattc caccgaagtt cagaaacttg agagagaact gattaaccaa
     1501 caaacagcgc aacaacgttt gtcaaacgaa attgataaaa cgagtaatgc actagctcaa
     1561 gcaaaaggcg aaatacagac gtacgagtct acaatgcaac agttggatag tgaacaaaaa
     1621 aatgttcaag ctagtgcttc tctgattgaa tccgaataca aaaaatggca agcaactgct
     1681 ggtcaatcag cttctgaatc cgagaaatta gcgaaagccc aagaatatgt ttctcaacaa
     1741 tctgaaaatg cagagaaaac gatagatatc ctgagacgac agttagaagc tacacagtct
     1801 gagtttggcg ctacatccac agaagcaatg cagatggagg cgaagcttaa tgatgctgaa
     1861 cgtgaatttg aagagttagg acaagctgct aaaaatgtag atacaactaa cttggacgat
     1921 atcggaagca aaatagatat gaataatcta atggaagctt ctgacgtttt aagcgacatt
     1981 ggcgataagc ttacagaatt agggaaacaa gcagtggact ctgctaatag tgtaggtagt
     2041 tcccagagta aaatacaagc taattttggt ttgactaaac aagaggctga agaattaacg
     2101 aatgtagcca gagacattta ttataaaggt tttggagaat cgttagatca gtccacagat
     2161 gcattgattt tggtaaagcg taatttaggc gatttaaata atcaagattt acaaaatatc
     2221 acggaacaag ctatggtcct agaaaacacc atgggcgctg atatggatga aacgttacgt
     2281 ggtgtaaatg gcttaatggt caatttcggc ttgagcgctc aagatgcaat ggatttaatg
     2341 gtttcgggta ctcaaaacgg tttagataaa acgcacgaat taggcgacaa tatggcagaa
     2401 tatagccaat tatggagtca aatgggatat tcagctgatg aaacgttcgg aatgcttcaa
     2461 aatggtttag atgcgggtgc ttataacctt gataaagtca atgacttagt taaggaaatg
     2521 ggaatatcgt taacagatgg tcgatttgag caaaacatgg atatgtttag tgaaagtact
     2581 agaaaagctt ttgaagagtg gaaaaatggc ggaggaacac aaaaagacgt tattaattcc
     2641 atgattcaag attttagcaa tatggatggt caatacgacc aattaaataa agcttctaca
     2701 atttggtctg cacttggcga agataacgcg atgaaagttg tccaatcttt gactgatgtt
     2761 aaccatacat ttgatgatgt tagtggatct gcacaaaaaa tgaatgaaga ttctactact
     2821 ccgttacaag aattaaacgg aaaaatagct gaattaaagg attcattagc tcctataggc
     2881 aacacaatca tagatgcact cgaaccagta attgattttc taggaaagat ggctgatgcg
     2941 tttaataatc ttccacaacc agtacaggat tatgccgtag cgattggcgg attgactgct
     3001 gcatttactt tattaatgcc aataatagtt ggcttcatgg ctctaggtgg tcctactaca
     3061 ttaataatag gagcagttat tactgttatt gctggagtta tagcaattat aaaaaactgg
     3121 ggcgcaatta ctgactggtt taagggaata tggagtaaat tcactgattg gttgggtggt
     3181 acttgggaaa gtataaaaga aggtgcctca tcagtttggg atggagttaa agaaacctgg
     3241 tctggatttg tagattgggt tcaagatatt tggcaaggag tttctgattg gtttggagag
     3301 ctatggagcg gattagttga aggagcttcc aacatctggc aaggagtcca agagacttgg
     3361 caagcattcg ttgattgggt ttcaaatatt tggaacggag tcaaagaagt atggtcgatt
     3421 atttgggcag acattgtagg aattgttcaa ataccatgga ccttaataac gtcattgatt
     3481 caagccggta ttaatattat cgtgggtatt tttgatgtag ctggacagtt attaggcgca
     3541 gcttggcaag ctgtttggac acctatttct gatttcctta aaaacacttg ggatactatg
     3601 acacaatggg taagcatcgc ttggaatgga attgtaacta cattccatac tatatttgat
     3661 ccagtagtgg catggtggaa tggtatatgg acagctatta gtactacggc ttcaaatatt
     3721 tggaattcaa ttagtgcaac agcttctagt atttggaaca gtatcaagaa tacaatcact
     3781 agcttggtac aagcagctgc tacagtaatt caaaatattt ggtcaactgt atctagttgg
     3841 ttaggtggaa tttggaattc aatcagctct acagcatcaa atatctggaa tagcgtgact
     3901 agtagtataa gcaatgctat aaacgcagct aaaagtgcca ttcaaagtgt ttggaatagt
     3961 atatcttcgt ggatcagcgg aatttggaac ggtatcaaaa acactgcttt gaatctttgg
     4021 aatggaatta caagcactat tagctctaaa gtaaacgatg gaaaaaatgc aatttcaagc
     4081 ggttggtcca atctaacagg tattgtttcc gacatattca ataatgttaa aagtacaatt
     4141 gctaacattt gggaaggtat caaaaagact gttagcgctc caattgattg gatcagagat
     4201 aaaatcagta gtatctttga taatttgaat atttctatac cacatattcc gttaccacat
     4261 tttaaattga gcggagaatt caatccattg aaggggaaaa tcccaacgtt gggtgttgat
     4321 tggtatgcga aaggtagtgt gtttaattct ccgaatatta tcggtgtcgg cgaagcagga
     4381 cctgaagcag ttttaccttt gaaaagatct gtgctgcaag aaattggtga tcgtatcttg
     4441 agtagcacat cagtttcatc tagggcacaa acgattcaac ctgtgaataa ctacgaattc
     4501 aatttcacaa ttgatggtaa cgcagatgag gttactatga agcaaacaac tcaacaaatc
     4561 attgatagca ttacaaaagt tcaaaatgat aatgcttcgg catggcgtta aacaggagag
     4621 tatttctcct gtttttttag tattaaaaag gatgtgaaaa aatgactgat tgtatacatt
     4681 ctataatcga tggatttcct gattatttgc ataaattggc tttagctgaa agaccaacca
     4741 taccttctcc aaaaagacag agagttgaaa cttctgtttt aggaaggtta ggtggcttag
     4801 tacaagatta ctcgtttgaa gacatgtcgt ttacattgca ctataactat ttagaggatg
     4861 tggaagacca tcaagcgttc aagcaatcgt tttatatcat gcgtcattgg ttaaattatg
     4921 caaagaaatt agaattctct gatgatccca acgtctatta tgttatccag actatcgata
     4981 ttggggatgc agaaaacgat attgttgaat ggggagagtt cgatgtaaat attactgcga
     5041 aaccattcgc aagagttcaa gaagatgtac ctataaccgt agataaacca cagtcattta
     5101 acttgctgaa taatagttta gaagaaagtt ttccaaagat tatcatcact ccttcagcta
     5161 cttcatgcca gttcatctta aatgattatg tgtttagttt tgaaggctta gtagtaggaa
     5221 ctgacgtagt cattgatagt gatttgatgc tttgctacga agagcaatcg gacggagata
     5281 ttttagatcg gtccaacaaa atgaagacca tgcaatatcc gacattgcaa gtggatatta
     5341 attattttaa ttgtactggt ttgagcaaaa tacaaattta tcgtaatggg ttaaggtagg
     5401 tgaaatagat gatcgataat ttaataacta tttacgataa aaacgacgcg aataatttag
     5461 ctgaacattt atatgatacg caaggtttag gcgctttgtc agactggtta acagctactg
     5521 ttagcaataa attaaacgga gccgagatat ttcagggtac ttatccaata agcggaacta
     5581 atgcagattt gattgtagaa ggacgtatta ttcagtgtta tgtagatgaa aatcgagcaa
     5641 aacagcgtct acggatttat tatgcaaaga cttctgtaat aggcaatacg atagaagtaa
     5701 aagctgaacc tattttcaat gatataagaa aatcggtgtt gaataaatat gacagtggaa
     5761 cagaaaagat cactgctagt caggcatggc aaaacgcaaa aactttagcg aaaccagtta
     5821 ttccttcaca gttttctttc tcgtcattag tagatacgct tgctaatgtg aagatagaaa
     5881 aggcgaattt tttagaattc tttggtggaa aagagggatc tattctagat cgatttcatg
     5941 gggaatttct aaaagataat aacacattac gtcatgaaaa aaggctaggc acggatcata
     6001 aaatcaaagc gatttatact aaaaacttaa ctggtcttga cttagagatt gatgctcaaa
     6061 gtgttttagt tggagtttat ccattcatta gcagctcttc agaaggagaa gacgagatca
     6121 ctctaccaga agaagttatt ttcacggatt acgtggatga ttatcctgct ggatatgttt
     6181 cttttgttga ttttaaagac aaagcgactg atgtagccac attaagggaa gctgctaaag
     6241 actggttgaa aacaaacata gataaacaaa aaccacaagt gagtggttcg attgaattag
     6301 taccattgag gcatcaaaga ggctatgaaa aatttgttga tctagaaaaa gtttcgatgg
     6361 gtgacggagt agatgtgtat catccacagt taaaagtgaa tatgtcagcg agaattgtgg
     6421 aatatacgtt taatgttcta accaattcat acgataaatt agttgtagga aacgtcaaaa
     6481 caaacttctt agaaaacaca gagaataatg tcagcaattt gattaatgat gccattgatc
     6541 aattgaaaaa cggtggcgaa atcagtgatt tactcaatga tattgtagat catcaaactg
     6601 atatgattac tggtcaaaat ggtggttatg ttttattaga tcctaaagaa gcgcctagtc
     6661 gtattttgat tatggacaca ccagataaga ataccgcaag gaatgtttta caaatcaaca
     6721 atgctggtat tggtttttct aaaactggca ttaatggaac atatgaaacg gcatggacgt
     6781 tagatggcgg attcaatgcc tcgtttatta cggctggtga aatagtagga attactatta
     6841 gaggtactac attaattagt gatggtgctg attatagaac aagtattgct aatggcaaaa
     6901 tgacttggta ctcaaaaaaa gttaacaaag atattatgga gctagaagca cgtgattatg
     6961 taagtgctga tgccggtatt gtatcataca ccatgaaaac tggtggtggt ttcatgatta
     7021 gaaatccaca gggtaacttg gtttttagta cgtgggataa tggtaataac agaccgtttc
     7081 tatcttttgg tgcgcccaat ttcaggtata gcaatgctag ttatgtaact tctggcgacg
     7141 gtagttcttt aagcattaat ggtagtgcgg gtaactcatg ggaatttaag gtagctggta
     7201 ggactatgaa atttactagt gatggtatgc taacgttacc aggttgtttt tttggttcat
     7261 gggaagatgg gaaacttgct aggtttgaac aatcaacggt acaagtatat aaagatttta
     7321 ctgttagagg tactaaaaac tcaactgtac caacagaaca ttatggacaa cgactattga
     7381 acgcttatga aactccagaa tattatttcg ctgattatgg ggaagccgtt acaggtgaca
     7441 atggtaaagt tcgtgttgat attgacccca tgtttgctga aacagtaaat ctaagtcggt
     7501 atatgacaca tgtgacacct acagaactag ttttgtgtgc tgttactcat gaagatattg
     7561 accatttcat cattgaaact agtaagccaa acgtattagt tagatggaat ttagtggcac
     7621 accgtctagg gtatgaagat attagattaa aagaggatac agcatatgat agcacagtgc
     7681 ttgaccaaaa acgtttttaa aacgaagaca aggaggtata taaatggcta gcagtttata
     7741 taatttggct ttagatttca gcaaagaatt aaactacacc aaagctatta tggctcgtca
     7801 aggtgataaa gggattacgg tgacggttaa accgtttcta aatggcttgc agatggatac
     7861 gagtggcgga acatttactt taaaaggaac aacaccatct aaccgttacg tagataatgt
     7921 tgcaactagt gtaactagtg aagaagtcac gttttctctt gatggcacat ttatgagtga
     7981 agcaggatat tataaacact gctacgtaga atatagaaaa gacaatcaaa ttttaacaac
     8041 gcaagatatc atttttttct cactaggagt gtctgacatt tcgcaaggcc aagccgatga
     8101 atatgtttcg caattagaag agttgattcg aaagtacaac gaaacttttg atgcttttat
     8161 ggctgaaatc aaaggtagag tggatagctt aaatcaacag attactgatt taactggtca
     8221 agctaaaacg ctacaagaca agttagatgc tctgaaagaa gaaatttcta agttaggtaa
     8281 cttacaagtg atgtacagta acagcatcga cttcgggggc tacgattata gcgggaatcc
     8341 gaatttaatg gctaatataa acgctgacag cttctcgcaa ggtagtggtg ccttatctgt
     8401 tgtagatgat ggtgacgagg tagtagtcac gcttgaccca aacaataaat tagagatatt
     8461 caaagcgaaa agtcaaccag ctctggtagt aggaaaaaca tataccatga gcgtagaaat
     8521 tatgctagaa ggcgatttca ctggagaccc tagcaagata ggcctaagat atattaaaat
     8581 gcctaactgg gtatcagggc tatatacgcg taatacatta actgctacta agggtgtatg
     8641 gcaaaaacta actggcaccg ttaaaattac tgctgcgagt gataacgctg aaagctggtt
     8701 tataatgcta caaaataaag acgctaataa cagcctatcc ggcaaactac gtttgagaca
     8761 cgctaaactc gaagaaggct caacagccac accatatcaa ccaaatttac tcgatgcgcc
     8821 gtattatttg agtaaggtgg ctttgggtga gaatattgct gatcctgcag ttagcttccc
     8881 aattaaaacg agcgcatacc gtttatacgg tgtgaacatg ctagaagagt ttaaagttgg
     8941 tcaaagatat acaattacaa taaaaggaac taaaccagca actcaagatt tttgggctta
     9001 caacggtggg aatatttcat tagagagaat gactccagta gaagggttag ttgacgtgtg
     9061 gacttgttca tttactatat taaaactaga tagtagttca ccaagtctat tgagtatata
     9121 tcaaacacca caatcaacag taggctcatg tcaaattgac tggctcaaga tcgaaaaagg
     9181 cgacacacga accccgaata ttgagcaata taaataccga ggaatcggca tgcgagactc
     9241 aaacaatcca aaagattatg tttgggatct agcaccagaa tatgtcgaag ataatttggc
     9301 cacagatatt aaaatttctg aaattactgg taaagcaaac aattataccg atgggaaagt
     9361 atcggagatt aattcgcagt tgactgcttc aattaatgaa gtagacacca cagctaagga
     9421 tgctcaaaca aaagcgaatg ctaatgcgac tgctatagat gaattagaca ataagatcga
     9481 tgaacgcatt aatgatacag ctactaccac attaacagtt acaaacggga ataccggatc
     9541 agcaaagctt tatcgtgaag gaaaaacagt ttctatatat tttgtggctt taaacggaaa
     9601 aagcagtggt ggaaatgatt caacgatact aacaattcca gaaggctatc ggccaccaat
     9661 tagttttgag caactggttg gctcgataga ccgttctact ttgaacagtg ctcaactatc
     9721 tattggcgca gacggagcca ttaaatggag aagaaattca agttatggat cagcttattc
     9781 atttgttatc acttattcaa tttaagggag gaaatctaat gaaagtagtt tacaaatcaa
     9841 tcaagcctta cggattcgag caaatcattt tgaacaatca agaaaatatc cctgaaaact
     9901 gtacagagat taaaccacca gttcctaact ggagaccaag atttgatttt gataaaaaac
     9961 agtgggttga gttagctact gaagaagaga agagtggcac agcggttgac gatattgaag
    10021 atgtcgatca gttgaagcaa ttaaatgctc tactgacaaa acaattggcg atatcggtac
    10081 aggaacaaga aaaaatgcaa caaatgttag ctcaattgac gatggaagtc gcaagtatta
    10141 agaatggagg gaaaagtaat gaatagtttt ccgggctttg aaaatataaa acaattttat
    10201 gattggggat gttatacgga ccaagattta cttgactatg taaacatgaa ctgtttaaca
    10261 aaagaccaat ataagcagat tactggaaat gaaatttaat taagcacaaa gttaaataaa
    10321 aagcgtactc aaacgagtgc gcttctttgc ataaaggaga aatagatatt ggaaaaatat
    10381 ttaaatacac tctcagtaat gacaggggtg ataggcggga caattgttgg tttattggga
    10441 gggatggaca atatactaca tgttttaatt tttcttgtgg gtgtggactt cttaactgga
    10501 cttgctaagg catggaaatt gaaagaaata agtagtgaga taggtttcga aggattgttg
    10561 aagaaagtct taatcttcgt tgtgattgcc gttgcggttg acgtacagaa aatcgttggg
    10621 aattctatac ctctaagaga aatagttatt atgttctatg ttgcaaatga agggattagt
    10681 tttttagaaa acatttcggt attccttcct ttgccagata agttaaaaga ggtattccaa
    10741 caaatacgaa atgatactga aaataaagac cgaggaggaa ccaaatgaaa aagaaaatct
    10801 ttgtaggagc tattatagct ctttttttat tgccaataaa cgcctttgct tactcgatca
    10861 acaatgaatt caatttgggc gcaaacgaag gtagttctca agtagcaaat aaccaataca
    10921 tcctattgca tgaaacagca aacgaaactg cgacaggacg aaatgaagcg cagtacatga
    10981 aacgttcttg gtacaatgcc tatacagcgt atattgttgg cgatggtggg atcgtttacc
    11041 aagttggaca acctggttat gtacaatatg gcgctggttc atatgctaat gcaaatagtc
    11101 ctgtgcaaat cgaactgcaa catacgcacg ataaagtaac gtttgaaaag aactacaagg
    11161 catatgttga attagcgaga gattccgcta ataaatacgg tattccactt acattagaca
    11221 caccgtataa tcaaccagga atcaaatcac atttatgggt aactcaaaac atttggggcg
    11281 atcacactga tccgtatgga tacttatcag aaatgggtgt aagtaaggaa aaactggctt
    11341 acgatttagc tcatggattt acagacgaaa acccaacgac ttctgagaac aagcctgtca
    11401 ttgatccaac ccgagcaggt gcagcaaatc caactttatc agatggaacg aaccattctc
    11461 acattgatca gtttggagaa atcgaaaatg cgaacttaca cgtcgctgga tggcacatcg
    11521 ctaactataa atacgagtat attttcatta tggactacaa tactgggaaa gaattagctc
    11581 gagtaagagc tgatggaatt tatagaccgg atgtaaatca agcttataat acttctggaa
    11641 atgttggtta tcatgtatct ttcaatatgc gtaattttcc tagtaagaaa gtctatgtaa
    11701 tgatgcgggc aacgaatgat ccagagggaa acactaaagg cggtgcgcaa gatttccatg
    11761 acaaacgttg gtatttaaat attcctaaac gataaaaata gctcctcgtt gaggagcagt
    11821 acataactat attgacaact ataaaaatta ttcgataaaa tagtgatgtt atcgcatatc
    11881 ttcactatca cccataaata gtcacactcc aagctatgcg ataacaggtt tgttgccaca
    11941 cattctactg gttgattgtt tatggcttta tgtggcaaca accagtaccc ttagctcagt
    12001 tggtcagagc agacggctca taaccgtccg gtcgtaggtt cgagtcctac agggtacatt
    12061 aacgtagcca tttgaatcgt tctgtgttag aatttttttg aagagtatta tacaagctaa
    12121 agcttttctt cattgccact caaatgagtg gcttttttat gtatcctttt atggattaat
    12181 gaaaggatgt ttcacatagt tatacttctg tatatttgaa aagttttact ttgattttta
    12241 aatagaaaga catttgggtt aaattgtgag ataataataa agaagagttt aaagcgcacc
    12301 ccaaaccact tccccataag tgtgttacgc tttaaactct tttatatttg aagccattaa
    12361 aaagcatacc atatttttga aaaaaagtga gaaaaaaggc ttacaattgg agtggtagtt
    12421 aattagtgac ttatttttga ttttatagca ctgatactat aaaatataga tatcatcata
    12481 ttacacaatc ttaatactaa cttaaaaaat atctcctttc acaagtatgg tgataaaatt
    12541 cgttccgggc taccttttta ggtagcctac tttaatcttt atacctttct ggatcaacga
    12601 aagtatactt tatatagtca taacgccgat gatcgctacg tgcgtccggc acgtcagtca
    12661 tgaatcggct acactagact agacagaaaa aataaggtgt gtagaataat aaaaaacaca
    12721 ctggaggatt tttcatgtca agacgtcaac gaagaaccta ttcaaaagaa ttcaaacaac
    12781 aaatcgtcga tctctatctc gctggtaagc ctcgcgcaga aattattcga gagtatgagc
    12841 ttacgccttc ttctttcgat aaatggatga agcaagcaca atcaacgggc tcattcaaag
    12901 aaagagacaa cttaacacca gaacaagcag aattgatcgc actaagaaag aaaaataagc
    12961 aactcgaaat ggagaatgat attttaaagc aagcggcgct gatattcgga cgaaaagaca
    13021 agtaattgat gccaacaagc ataaatattc catatcagcg atgtgcaaaa ttctaaatat
    13081 ttctcgtcaa acctactatt atcaagcgaa accgatcgaa aatgagtccg acttagaaga
    13141 gatcgttcag gaagagttta ttcgaaaccg aaaggcttac ggtacccgaa aattgaagaa
    13201 gtgtttagca aagcgtgggc ttcaactcag tcggcgccga atcggtcgaa tcatgaaacg
    13261 ccgcggattg acatctacct atacgatcgc tcattttaaa gggcaacgaa cagcttgtaa
    13321 tgaagcgaaa acagcgaatg tattagatcg gacctttaca caagaacagc cattggaagc
    13381 catcgttacg gatcttactt atgttcgcgt ggggaaaaag tggcattata tctgcttaat
    13441 acttgatttg tttaatcgag aaattattgg ttattcctgt ggtgagaaga aagatgcctc
    13501 attggtaaaa gaagcctttg gacggatacc gtattcttta acagacgtca agctttttca
    13561 tacagaccgg ggaaaggaat ttgataacca aaccattcat gagattctga atggttttgg
    13621 aattactcgt tcattgagta ggaagggttg tccgtatgat aatgccgttg tggaatcaac
    13681 ctataaatct gtcaaagtag aattcgtgca tcaataccaa tttgagacac tggcacagct
    13741 acgtctagaa ttgtttgatt atgtgcattg gtggaactat ctacgcttac atggcacgtt
    13801 ggcgtatgaa acaccgatcc aaattcgaca acagagattg gcgaagcgaa tccttgataa
    13861 tgagcgcgga tctgatacct ctggagaggc agcgtaattg aatgattgtg cttctgccgg
    13921 agaaaatcag atccgaggat gctcattgtc aagggcaatc gtagccataa caccgcagca
    13981 ttcacaacac cttataattt ttgtc
//
LOCUS       DACDIE010000061        13826 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.061, whole genome shotgun sequence.
ACCESSION   DACDIE010000061 DACDIE010000000
VERSION     DACDIE010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13826
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.061"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..295)
                     /locus_tag="IV250_002188"
     CDS             complement(<1..295)
                     /locus_tag="IV250_002188"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP8769439.1"
                     /translation="MSISFSNNGRKSFSGKEVQIQYAALDIYWNIFSSTATEFYDEDP
                     QVVAFMMNTFFKKEVLDRLNSGMIDKLYFLLKICKENFPATSVKDVQKEFTEYE"
     gene            complement(302..736)
                     /locus_tag="IV250_002189"
     CDS             complement(302..736)
                     /locus_tag="IV250_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769440.1"
                     /translation="MQTQEAIKTFILKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISFSQQTNYSASYLYTKMKKINAFLA"
     gene            884..2025
                     /locus_tag="IV250_002190"
                     /pseudo
     CDS             884..2025
                     /locus_tag="IV250_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(2151..3230)
                     /locus_tag="IV250_002191"
     CDS             complement(2151..3230)
                     /locus_tag="IV250_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP8769441.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(3333..3773)
                     /locus_tag="IV250_002192"
     CDS             complement(3333..3773)
                     /locus_tag="IV250_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8769442.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(3830..4240)
                     /locus_tag="IV250_002193"
     CDS             complement(3830..4240)
                     /locus_tag="IV250_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP8769443.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(4420..4854)
                     /locus_tag="IV250_002194"
     CDS             complement(4420..4854)
                     /locus_tag="IV250_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP8769444.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(4954..5802)
                     /locus_tag="IV250_002195"
     CDS             complement(4954..5802)
                     /locus_tag="IV250_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8769445.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            6191..7957
                     /locus_tag="IV250_002196"
     CDS             6191..7957
                     /locus_tag="IV250_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAP8769446.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPVHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            8059..9318
                     /locus_tag="IV250_002197"
     CDS             8059..9318
                     /locus_tag="IV250_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP8769447.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            9465..10766
                     /locus_tag="IV250_002198"
     CDS             9465..10766
                     /locus_tag="IV250_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8769448.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            10769..11620
                     /locus_tag="IV250_002199"
     CDS             10769..11620
                     /locus_tag="IV250_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8769449.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            11633..12448
                     /locus_tag="IV250_002200"
     CDS             11633..12448
                     /locus_tag="IV250_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HAP8769450.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            12599..13765
                     /locus_tag="IV250_002201"
     CDS             12599..13765
                     /locus_tag="IV250_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8769451.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
BASE COUNT         4727 a         2466 c         2549 g         4084 t
ORIGIN      
        1 attcatactc tgtgaattct ttttgtacgt ctttcacaga agttgcagga aaattctctt
       61 tacatatttt taatagaaaa tataatttat ctatcatacc gctatttagt cggtcaagta
      121 cttctttttt aaaaaaagta ttcatcataa aagccactac ttgcggatct tcatcataaa
      181 attctgtagc agtacttgaa aagatgttcc aataaatatc taatgctgca tattgaattt
      241 gtacctcttt tccagaaaaa gactttctgc cattattaga aaaggagata gacaaaccgt
      301 actatgctaa aaatgcatta atctttttca ttttcgtata caaataagag gcactatagt
      361 ttgtttgctg actgaaactg ataatcgaat ttgtctgaca gctgaacaaa gaagtcaaga
      421 tctgataact gactgattcg ttgcagtatc ttccaaatag acgataatag cagtaagccg
      481 gatcaattcc ttcaaaattc aactttaaaa aaggtttttg cttatcaata tgcagttctg
      541 ttagattgag acgctcaaag tcttgttcta attctttgat gtatagtaaa aaatttgatt
      601 cagacatatg gaaatgatct gctaaatatt gtgtagtaat aaagaaacga tgttcagata
      661 ggaaaagaaa aatatctaat tttgtattgc tttttttctt caagataaac gttttaatcg
      721 cttcttgagt ttgcatgaaa gaggctcctt tttacttaaa ttgttaaaac ttgaaaatat
      781 tactgttttt ttcataaatt tagacaaaaa aacagtaaac atataggtag aatctctact
      841 atcttatttc ttgcatacaa aaaaatcaag gagtgagaga gttatgagat attcaaccat
      901 cttaatatca acatttgcag ccttaagctt atcgcttact ataaatcaga cagtccacac
      961 agaggagaca gaaacagcca aatggacaga aaccaagtgg gccaacgtca atcgaattac
     1021 ctttcaggat gaattttcat ccttcaattc aggagtcgca aaattaagaa aaggacaaac
     1081 agcgaccatc aaagcaaaaa ttgactattc gggagactta gcacctatac tacaatcagc
     1141 tgttacattt gagaatgttg atcccacttt gtcgattggc tatgacgaga acactttgac
     1201 tatccaacac aagcaagcga tatttacctt caccgttaca ttaaatcagg acttgttaaa
     1261 accagcgctt tttactgtca aggtatcaga tggactaccg aacgatcatc atttaacgcc
     1321 gtatagtcag cggcagacaa ttgagcaaga ttcagccatc gactcaggag gagatcttgt
     1381 tgaagaacca acagataaac cagaaaatga aaataagcca gaagtaccgc ctacagaaaa
     1441 tccagacgga gaacagaaac cggaaataga acctggagaa gaacccgata cagaaactca
     1501 accagagcca gacaataaat caaaacctga aataacccct ggagaaaaac cagatgtaac
     1561 tccagaacca gatacggatt ctggaaacca aacagtaccg gaaacaaatc cagacacaga
     1621 taatgaaact gaaaatccag aaaaaccaga ggtagatcca gaagaaaaac cagatgtgac
     1681 tccagaacca gatacggacg caagagacca aggaatacca gaaaaaataa acaaaaaaac
     1741 aattcaagag gatggaaaaa aagagagtaa aaaatcaaat ctagcaattc taaaaataaa
     1801 tgaagagcag ctgaataaaa aatcacgaat atttgatagc gcccaatcag ctgaaacctt
     1861 aaaaagtagc aaggatacaa cctttgctgt cctgaaacga aaaataaaca gctaccaaaa
     1921 tccggagagt cacaaaacaa agttatactg tggagcggaa tcattctatt aagtatagca
     1981 acaatgcttt ctgcaaaaag atttaaacaa aacagatctc tctaacattt gaataatgtc
     2041 tgtgaacgca tttttcaaat aaaaagaaat atccagaaaa tgaccccaaa ataccagatt
     2101 gaaatctgat atttggggtc gttcatactt tatctttctt catatttatt ttaagggata
     2161 tctgtttctt ttggaagatg gttcgggatc tttttcactt tcaatacatc taaaaagaag
     2221 gtaaagatcg gaatgccgac aatcaagccc caaacgccaa agaaccgttc gctaacaagt
     2281 aagaccacaa atgtataaaa aatcggcagt tctgttttgc tggacatcaa cttcggattc
     2341 agcacataag actcaattag atggacaatc aaaatcgtaa tcaggatata aacaacatct
     2401 cggatgcctc cttgggaata agctatgaaa gatagaggaa tacaagaaat gataacacct
     2461 gctactggga tcagacttaa aatgaaaatc atgatcgcta aactcagcag ctgatgaaag
     2521 ccaaatacag ccaaggcgat tgtagtgagc aacgtattta aaagagcgac aacaaattga
     2581 gcttctaata ccaaaccaaa tgtattgaca aacttatcgg caaaatagta aatatcttga
     2641 aagaaccaag aaaattcact ttttaaaaac aatcttgaga atacaattgt ttttttcttt
     2701 tcaatcataa aaaagaaact taagatgaat gacatagcaa acgccactgc catttttcct
     2761 atatcctgta aatagccaag cgcaatcgaa gcgccatttt gaagctgttc aaaaaaatcg
     2821 tttcgctcaa tataagtatg gatatattgt aaaacaggat ctgcatcttt tggctgatgc
     2881 tcgtaaaaat taataacgga ctggatcatg tcaaatgttt gatgaataag catcgggata
     2941 tactttgtga atgccagata gataaggtaa acgatcagtg tataaatcat gatagctaaa
     3001 acacctgtag ggattcggac aaatcgctga atgatttgaa caagatgaat catcaaataa
     3061 gtaaagataa atgtgagcaa aatggttgta atcatacttc taactaagaa aagaacaaaa
     3121 acgacaaata ataaaacagt gagacggcgt attttttcat tcgccaaaaa acgctgataa
     3181 acggtcaaat cgacaacctc caaataacaa aaaataaaca cgttattcat tatatgatgg
     3241 aatatgaaaa taagaaagag caaaaagaaa aaccgctaaa tgacgttatc ttatgagttg
     3301 gttttcctca taaaatcgtt catctagcag tttcagccct ttttctggtc ttgatgaaaa
     3361 ttatctagca tttttcgggt cagatgttgc agtgtatcca attcttcctg ttcaagtgga
     3421 agataagact tgatcttttg tggaatacct tctgcttgtt tttgcaacgc agcaccttga
     3481 gcagtcagcg taacatttac gattctttca tcacccgttt cacgctgacg attaatataa
     3541 tttttttctg ctaacttttt tagtaaggga gtcagcgtac cagaatccag acacaaatca
     3601 tttcctagct ctttcactga agtctttttc ttttcccata aaactaacat cacaagatac
     3661 tgggtatagg taagattcag cggcttgagg aggggagtgt agtgtctaat gatttcttta
     3721 gaggcggcat ataaaggaaa acatacttga ttaactaaat atagatcttc catttttcca
     3781 cctccgctgt tttactatag tgtatcatag cggatttaca caaaaaagtc tattcactaa
     3841 ctgctttcag agtaacttct atatttccag ctgtagcttt tgaatacgga cacactttat
     3901 gcgctgcctc taataattct tgcgtttctt ccagagaaag accttcgata gatccttcaa
     3961 tctctacacc taagacaaag ctcgtttcac tttctgaata aagggaaact tgtgcactga
     4021 cagtagaagc cccagagata tttttttgct gtttgatcaa ttctaatgca ctgttaaaac
     4081 atgcactgaa accagctgca aacaattgtt cagggttggt tgcattttcg acgcgtttac
     4141 caggagctgc aatattcaat tcgaatgtac gatcgggatt atgcacttct ccagagcgtc
     4201 cgcctgtgtt gatcattttc gtagaataca tttttttcat aaaattacca cctttaatta
     4261 tattttatac aattgaattt tacacaatat aattgtggaa agcaaagaaa tggattggca
     4321 taagataaat aagagaaagc acatgaaaca gacgcgcttt ctcgaatagc aaacatatac
     4381 aaaaaatatc agagacaaaa aataatctca tcataaacat tacatatatt tctcaaaaag
     4441 ttgttttctt tcttcaaaaa ttacttcacg atggacctga tgaagcatcc ataaataacc
     4501 aaaaggatct ctgaacattg agttagtcac accaaagtcc atcatttctg tcatttcttg
     4561 aatctctaca cagccatttc gtaatgcttt attatgtgtg tcctcgatat cagagacgat
     4621 gatattgaac cagatcgtat ttggatgttc agcagtagga gcagtcaaac caagagacgt
     4681 gttttcatcc agcatatgga atctcgtacc atacaaggta aaaatgactt cattattgcc
     4741 tctagggtaa tctgttgcct caatcagttg tatttcatca aagattgttt gatacagttc
     4801 tagtgctttc aaactatcat ttaatacaaa atcgatttcc gttcctttat gcataaagat
     4861 ctcctctcct tttcagtcga acagttacta gattctttta ttataccagc taaagaaaac
     4921 gcattcaaaa aaatagcgga tttacttacc aactcattcg attggatgat aagtaaatcc
     4981 gcgtcccatt acttctgcta cgctgctgac aatgacaaag gcacttggat cgatttcttc
     5041 tatcaatggt ttcattcggg aaaattcatg atcagtcaca acgatcaata aaacctcttt
     5101 tgactttcga ttatacccgc cttttgcctc tagcagggta aggccacgtc caatctcagc
     5161 acttaagcga aggcggatgt cttccaaata atgttcactc atgaccataa cagatttctt
     5221 tcgattgata cctgtttcga tataagtcat gacaattgaa gtgattgcaa tagataaaat
     5281 agcataaaga aactcttcta ttccaaaaat aaacagatta agagaaacaa cgaatgcgtc
     5341 tgtaaaaaat aagccaatcg aagtattcaa gtggaaatat tttttaaaaa tgaggggagg
     5401 gatcgtcgta ccgccgctag aagagttgtt tttatataaa atcgcaactc ctaaagcaaa
     5461 aatcgcactt ccaaaaatga cagacaacaa ccgatctgaa gtaatcattg tttctggaac
     5521 tattgcaatg attaaaggaa aaaccaagct tccgaaaagt actttaaaaa agggtttttt
     5581 acctaaaaat agaagagcta gtaccaacat aacgatattc aacactaaaa taactatagc
     5641 tcggtcaaag cctaaagcag cttccagtaa tattcctaga ccgctgacac ctccagcagc
     5701 aatatgatga ggccctaaaa acatattgat agcgactcct aatatgaaga gggcaaaaat
     5761 aatcttaatg tatgtccata taaattgtct cgtttctttc atttattcac ttccttaatt
     5821 cattaataat actcttatct tattataact tacttttcac cttaagttaa ctttaattaa
     5881 taaaaaaatt aggctagtct aaattattat gtttcattct tctagaatat acttctacgc
     5941 caaaaactgt taaaaaactt ttactttaga aaattggaga aattgatcgc ttaagataaa
     6001 ttcaggacac taaatagtac aggaagtaga ctaaaacttt taaaagtttt aagtctatct
     6061 atttggcaag tttaaaaaat aaaaattcgg tatcaaaaaa ctgttaccga taacattttt
     6121 gaaagcgttg tcttttgctt ctctatcact tatagtgatg acgtcatatt aaaacacaag
     6181 gaggaaatca atggatacag ctgcaattta tcatcgaccg gaaagtgaat atgcatttct
     6241 atatacgaat acaagatttc gtattcggct tcgtactagg aaaaatgata tcaaaaaagt
     6301 atatgtgcta tgtggagatc catataccat aacaactgaa aaatggtatc aaaaacaacg
     6361 accaatgaaa aaatgtctaa gtaccaatgt tcatgattat tgggaaattg aagtgacgag
     6421 cgaaacaagt cgcttacagt atgcgttcca tgttgttggg gaagataaca cggactgttt
     6481 ttacggtgat caaggaattt ttccttatcg agaaaatgtg attactgaac caaattttta
     6541 ttttcgaata ccttattttc atcaaatcga tcgattcact attccagaat gggtaaaaga
     6601 aacagtatgg tatcaaattt ttcccgaaag atttgcaaac ggggacaaag caaatgaccc
     6661 cgtacacact ttaccttggg gaagtaaaga tccagaccga gaagactttt tcgggggaga
     6721 tttgcaaggt gtgatggatc atttggatta cttgacggac cttggcatca atggcatcta
     6781 tttttgtccc atatttaaag ccacatccaa tcataaatac gatacgattg attattatga
     6841 ggtggatccc gcttttggcg ataaaaaact tttaaaaaac ttgattgatg aagcacataa
     6901 aagagggata cgtatcatgt tcgatgctgt attcaatcat atgggtgtcc attcaccaca
     6961 gtggcaagac gttctcaaaa acggagaaaa atcgatctat aaagactggt tccacatccg
     7021 ttttttccca gtagattctt atcagatgac tgacttacca gaaactgctg aaaatatccc
     7081 atatgatact tttgctttta ctccattcat gccgaaacta aatacagcaa atccagaagt
     7141 gcaaaaatat ttgctggata tcgcaactta ttggataaaa gaattcgata ttgatggatg
     7201 gcgtcttgat gttgctaatg aagtcgatca tcatttttgg aaaaaattca gagaagcagt
     7261 tacagaaatc aaaccagaca tctatatcct tggagagatt tggcattcct ctcaagcttg
     7321 gctgcaaggg gatgaattcc atgcagtgat gaattatgca tttacagatt cgattaaaga
     7381 ttattttgca aaaaagaaaa tcacagcttc acaaatggtc agtggaatga atcatcagca
     7441 aatgctttac agagatcagg tgaacgaagg aacattcaac ttattagatt ctcatgatac
     7501 tgccaggatc ttgacccttt gccaaggtaa caaagaattg atgaagtctg tgatggcctt
     7561 tatgtttctt caaaaagggt ctccttgtat ctattacggg acagaaatag ggatgactgg
     7621 tgaagatgac ccggattgcc gcaaatgtat gatatgggag aaggaggaac aagatctaga
     7681 gctattcggt tttataaaag aacttgtttc tctaaggaaa cagttgtcta agattatttc
     7741 tgaaggttct actcagtggc taatagtaaa tgatcgtgaa gataagctct atttcacacg
     7801 gaaactagag ggacagatca tctatgttta ctttaatcag tcaaaagaac cttgggtagt
     7861 agaacaggaa aatgaggtga ttcttagtca aaattgtcag ttgttagaag acgggaaagc
     7921 agagatacga caatacggat ttttgatcat gtgttgaaaa ctgttaccgg taacaacaag
     7981 aaagcgtttg caaatgaaga tttgtgccca ttataataag ggtgtaacag cacgttgaat
     8041 acaaaggagg aaatgaagat gaaaaaaaga agtgtgaaac agttgggttt agggttaatc
     8101 tctttaggaa tcttaggcgg attagcggct tgtggcaatg gtaactcttc agcagaaggg
     8161 gaagccgatg aaggaaaaac gttaacagtt tccgttgatt ctggatataa agattatata
     8221 aacgaaataa aaaacacttt cgaaaaagaa aacgatgtga aaatcaagct agtagaaaag
     8281 gacatgttcg atcagttgga atcattagcg ttagatgggc ctgctggaaa gggacctgac
     8341 gttatgatgg cagcatatga ccggatcggt gcgttaggac agcaaggaca tctggcggaa
     8401 gtaaaactgg gaaatgaagc agcttacgat gaaacagaca aagcccaagt gacttatgat
     8461 ggaaaaatct acggagaacc tgcagtaatc gagacccttg ttttgtacta taacaaagat
     8521 ttagtcgata cagcaccatc aacgtttaaa gatttggaga atctatccaa agacagtcgt
     8581 tttgcttttg agtcagaagc aggaaaaaat acaggatttt tagctaaatg gactgacttt
     8641 tattattcct atggtttaat tgcaggttac ggtggctatg tatttggtga tgatggcaca
     8701 aatccttcag atatcggttt gaataatgca ggtgctgtag aaggaatctc ttatgcaaca
     8761 gattggttca aaaatgtttg gccaaaggga atgcaagaca taaaaagtgc aggagacttc
     8821 gccagccagc aattcatgtc caataaaact gcagcaatca ttgacggtcc atggcaagcg
     8881 caaacataca aagaaaacaa tatcaattat ggtgtagcca aaattcctac tctaaataat
     8941 ggacagcctt atcaaccgtt tggcggagga aaaggatggg tagtcagcaa ttatgctaaa
     9001 aacaaagacc tctctcaaaa atggcttgat tatgtaacga atcaggaaaa tcaagaaaaa
     9061 ttctttgaaa tggtaaatga aatccctgca aaccaacaag ctagggaaac agcaaaggct
     9121 aaaaacgatg aattaacaac agctgtgatt gaacaatatg aaacagctca agcgatgcca
     9181 aacatcccag aaatgggcga agtttggaca ggtgcagaaa acttgatgtt cgatgcagca
     9241 tcaggttcaa aaacaccaaa agaatccgca gatgaagcag taaaaacaat cagtgaagcc
     9301 atagaacaaa aatactgatt aaaatgataa ccgtccattt ttataaaaaa tggaacaggt
     9361 gcttcagata aaagtggcaa gagcgagatg gaaaattatc ttgctcttgt tgccattatc
     9421 ttaaaagcag tttgcggttt agcaagttta gagggggcat tcagatgcag caagataaaa
     9481 caagcaatat caacgaaaaa aaaatcacgc tttgttcact tattccaggg atggggcagt
     9541 ttgtaaatgg ccaagctttt aaaggtatcg tttttttcgc catattcgtc ctattcattg
     9601 gtcagatgct tctgggcgga tttcatgcat tggtaggatt ggttactttg ggaagtatac
     9661 caatggaaga tcattcatta tttattttga ttgaaggcac tttgcaattc atcattacaa
     9721 tcattttctt gattttttac ggtttgaatc ttttagacgc ttatcgagta gcgaagatgc
     9781 gtcaagaggg caaaaaaatc aatcggacga tgaaggaagt catacataat ataggtgatg
     9841 aaggttttcc ttatctattg acacttcccg cttatcttct catgattttt acgatcattt
     9901 tcccagtcct agttacattg ttcactgcat ttactaatta tgacttcaat catattccac
     9961 cagccagttt aattgattgg gtaggggcta aaaacttttt tagtatcttc tttttaagct
    10021 catatcgtaa tacttttttt gcagtctttt cgtggaccgt gatttggact ttatgtgcaa
    10081 caactttgca aatcaccctt ggtattctca cagcggttgt tgctcaccag aaatttatta
    10141 aattcaaacg tattttcggt atcattttct tattaccatg ggcggtccca gcttttatta
    10201 ctatcatgag tttttcgaat atattcaacg atagcgtagg tgccatcaat actcaagtga
    10261 ttcctttcat aaaccagctt ccttttgtag atatcagtgc aatctcttgg aaaacggacc
    10321 cgaactggac gaaagtagca attatcttag tgcagggctg gttaggattt ccctatatct
    10381 atgtgatggt tacaggaatc ttacaatcga tttcagaaga cttatatgaa gcagcaaaaa
    10441 tcgacggagc aaatgcatgg cagcgctttt caaacatcac gcttcctgct gtcttcctag
    10501 tggcagcgcc aacatttatt acacagtata cggggaattt caataatttc tccatgatct
    10561 atctgtttaa caatggcggt cccggaagcg taggcggagg agctggttct actgatattc
    10621 ttatttcctg gatctataaa cttacgactg gaacttctcc gcagtattct atggcttcag
    10681 caatcacgtt gattatctcc gtcatcgtta tctgtgtgtc attgctggta tttaaaaaga
    10741 caaatgcgtt caatatgggg gattaacaat gaaaaaattt cattcgagtg ctcgattcca
    10801 aaaacgactt tctcactttt ttacttactt ttttctttta atactgtcag tcattatcat
    10861 ctatccgctt ttgatcacag caagtactgc cttcaagtca ggaaatattg cagcttttag
    10921 tttagatctt tcaagtgact ggactttagg aaactttact cggctattca gtgaaacact
    10981 ttacgggact tggtataaaa atacattgat catttctgtc ctaacaatga tcgtacaagt
    11041 gactgtagta actttggcgg gctatactta tagtcgctat cgttttatcg gccgcaaatc
    11101 aagtttgatg ttctttttag tgattcagat ggtaccgact atggcagcat tgactgcctt
    11161 ttatgtcatg gctttcttat tagaagcgct cgataagtac tggttcttga caatgatcta
    11221 tataggcggt ggaattccaa tgaatacttg gctgatgaaa ggatactttg acactgtacc
    11281 aatagattta gatgaatctg ctaaacttga tggggcaggg catttcagga tttttttcca
    11341 gattgtatta ccgcttgtca aaccgatgct cgccgttcaa gcattatggg cttttatggg
    11401 accttttggg gatttcatgt tagctaagtt cctgcttcga acacctgaaa atcagactgt
    11461 tgctgtcggc ttacaaacat ttatttctga tgtgacaaac caaagagtct ctctttttgc
    11521 tgctggggca attttgattg cactaccgat ttgtctatta ttcttctttt tacaaaagaa
    11581 ctttgttacc ggcctgacat ctggcggaac aaaaggatag gagcagagta tcatgcaaac
    11641 atttccgtta aactattttt ccgcattacg tacgccaact caagtgtttg cgggacgcaa
    11701 actattatca tggcctaaat ttttcttgat atttgtattt ttagtttcct tgatggtcat
    11761 gcctgtgact cttttttatg ccaatcagat ccaagcgatt ccattagaac aatttctatc
    11821 cgttcattcg ctgatcgatg aacaaggaac ccaaaaattt tctgaacttg aattatctga
    11881 aactgggtta caagcatcgc aacaaacgat agctgtgacc cctgaaattt tggtaggtgt
    11941 gtctttatct gagaaacaac aatccgatca tggaactttt attgattttg agaaggaaca
    12001 atgggtgatc caacaaaagg ataaatctgg tatacgccgc tatacgatga attatagccc
    12061 atctttccaa ccagattctg tacgtacgcc cgaagatttc caacgatttt tagaaagaga
    12121 attttatgcc agtaatcgtc caacaatcat tttatcttat agtttaagtc ttggtcttgt
    12181 cctatttgtc atgacgagtc tcattttatt cggggcttcc tttttcttgt ggatgacaag
    12241 aaaaagccaa ctttcatcca ttcatacatt caaagagagt gcaaatttga tgctgaatgt
    12301 tatgggaata ggttccatgg cagcagcagt tgtagggttt atccactttg actttatctt
    12361 gatgctaagt gtccaaacaa cgataactgt cttgctgctt ttatgggttt ttgtcaaaac
    12421 aagattcaaa gataagcgtg tcgaatgaca agtttaagcg atttatgcga caatgaatgt
    12481 gaaaaaaatg aaaaataaag tagtttttga aaaatttctg aaaaaattaa acttcttagt
    12541 tgattctttt cttttttagt attaacgtaa tagggtagtt tttttaagga ggaaaatcat
    12601 gttagagaaa actttataca atcaattatt ttcacgttct ttctcgctcc ctgtcgaagt
    12661 aaattattgg gacggtacaa cgaaacaata cggagatacg gacactcctc cccaaatcaa
    12721 aatcaatatc catgaagaaa tcccaatgaa agagattaca agtaacgcat ctttggcact
    12781 aggagaagcc tatatggatc atcgaatcga gatcgaggga agtatccaag cattgatcaa
    12841 cgatgcttat cagcaagcaa acagcttttt acgaggaaaa gattatctaa aatggctgcc
    12901 aaagaaagaa aaacatacga aacaaaaaaa caaagaagat atccatgcgc attatgactt
    12961 aggtaacgac ttttataaac tttggctaga tccaactttg acttattctt gtgcttattt
    13021 caatacacct gaagatacgt tggaacaagc acaaatcaat aaagtccatc atatcttaga
    13081 caaactcttt atcaaagaag gcgaaacgtt gcttgatatc ggttgtggct ggggaacatt
    13141 gatgttcact gctaccaaag agtataatgt caaggcaact gggatcacac taagtgaaga
    13201 acaatacgat ttgattacca aaagaataaa agaagaacat ttagaagata aatgccgtgt
    13261 attattaatg gattaccgcg aactaaaagg cgaaacgttt gatcatatca caagtgtagg
    13321 tatgttcgaa catgtaggtt ctgaaaatct tgaaggttat ttcaaagtgg taaaagattt
    13381 attgaaacca aaaggaacag ctttgatcca tggaatcagc cgacaacaag gcggtgcgac
    13441 taatgcttgg atcaataaat atattttccc tggcggttat gtccctggtg ttactgaatt
    13501 agttgataat attacaaaaa acgatttgca actgattgat ttggaaagct tacgtcgtga
    13561 ctaccaattg acattagaac attggacaaa gaattttcat caagtccaag atcaagtcac
    13621 gaaagaaatg ggtgattcct tctatcgaat gtgggatcta tatttacaag cttgtgcagc
    13681 ttcatttgaa tcaagtaata tcgatgtgat tcaatatttg cttgttcaac ctggaaacaa
    13741 tgacatccca atgcatcgtg cataattgag gcaaaagtac tgtttaaaga ctatttaaaa
    13801 agaagctaag ttttatgatg ctccag
//
LOCUS       DACDIE010000062        13581 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.062, whole genome shotgun sequence.
ACCESSION   DACDIE010000062 DACDIE010000000
VERSION     DACDIE010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13581
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.062"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            214..2325
                     /locus_tag="IV250_002202"
     CDS             214..2325
                     /locus_tag="IV250_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP8769452.1"
                     /translation="MEGIMENKIHCTTGRVITLVLFLFLLLFARGTAVQALEKVDPSQ
                     IKENNNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAH
                     YQKVGTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQ
                     SINVMNAKLEFKYAGTKTKVALSEPQPEIQSKMRSMRAAGIDLNDTFLSIYSLNGQYQ
                     QRVSWYNDNNESVGERNIDMREFVGYEKMGSLPYFVTTDTACAEYKAPALSTNNLTSK
                     VVGGRAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTAL
                     LWTPQPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKS
                     FKITDTFDSKNFDMVSDGKNYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSF
                     LANAANYGRDYILQLHMRAKNDAKGIAPNVATVTFNNDPNKGTQKTNTVENYFSLKPN
                     KKVEQDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIASDHYKQFSIQD
                     PVEDRLTYKEGSARVIDMSTGKDITSEGTLTYDSNSRTLKWEASADFLSKNLLDGREI
                     QLIFTAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQIIVPKTGSNHLVLT
                     VIGSILFLTLASLGYVVLKLK"
     gene            complement(2363..4420)
                     /locus_tag="IV250_002203"
     CDS             complement(2363..4420)
                     /locus_tag="IV250_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAP8769453.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNNEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQTAL
                     SNAKKVLDDSKVSQTQVNNAKNSLDAAFKGLQHKPETPKEYMVTINHIDNQTGQVIAT
                     DQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQI
                     NLLCNNNYKNEGRVDILKEQIINVTHGESVTLTAPSIQGYVLDDRVEPTNSVTLNNVT
                     ESQNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFSTPWKNMTDQNYFLCRN
                     DDPTVTVDKNGQRSINNVDQNKTITFKYKKISLDELRNFMRQKELAWLNDYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENISQTGLYID
                     DLLSDDGAIDAMVGWKNSPAHNANLLYNNQSILGLGHNFVVDSAGRIGVDNVFISANP
                     IFK"
     gene            complement(4534..4746)
                     /locus_tag="IV250_002204"
     CDS             complement(4534..4746)
                     /locus_tag="IV250_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769454.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIVDINTSTNK"
     gene            4933..5400
                     /locus_tag="IV250_002205"
     CDS             4933..5400
                     /locus_tag="IV250_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769455.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLIHNLKIIDDTTWEYSESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIREFI
                     NKPDSLFIVVPYEKDGVKKIIFLGSSKDEKRTKEKLIHDGSELNKYKLQKTSE"
     gene            5397..5639
                     /locus_tag="IV250_002206"
     CDS             5397..5639
                     /locus_tag="IV250_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769456.1"
                     /translation="MIASLFIYPEGGITVAKVKTPFEKAASKILERRGVSEIQWKKEV
                     LEKAQFDLLRGKDKDLEEYIYQREREKLVFDEIIKH"
     gene            5657..5959
                     /locus_tag="IV250_002207"
     CDS             5657..5959
                     /locus_tag="IV250_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769457.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            6031..8085
                     /locus_tag="IV250_002208"
     CDS             6031..8085
                     /locus_tag="IV250_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769458.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLIDLIR
                     LDVFKDTDETMSVEESYRNEKLLQVLEEELESDQRKVNQVSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETDEVEVDPVEA
                     NEESTWTEGDFQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKVKEELNKEEQRVIEEEVQKVKERYEKKRDNAYSLCVE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLEIRKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKESDSSKAKEGSSDSTVQSNTSESSESNE
                     VK"
     gene            8085..10697
                     /locus_tag="IV250_002209"
     CDS             8085..10697
                     /locus_tag="IV250_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAP8769459.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPENEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSIGFPKYLDLKLHESLLNQRIVVEFYHKGK
                     KFHEERVKVGYRGFCEINFDCHLEQGDHVCLKYNHQGKQYVAWYEFSQKVLKDEEGKI
                     VYRKKRGETHLPELEKEAVLTLDEKKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSFENETAKKLLIQFYEEQATDRLQEYVEKLIKNEEISEMI
                     RKRLLLEAEKAVKE"
     gene            10694..13078
                     /locus_tag="IV250_002210"
     CDS             10694..13078
                     /locus_tag="IV250_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769460.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDADPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PDQAKGMAGNDKSGLSDIQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYISSPGIILAGESSLSVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAVGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDITHDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     VEDLTEKAKDTLEEKLDGTRAKEFAEAVKNPSEALEKQKEKASAVKEGLKDAYGEGQV
                     NAAVKANTTSEKENQEGAAIPSAKDHQDELHSNENSIGEKDSEEGQHGQLPKTDPEQY
                     RYKSNINSENEKRISDLPKRNQEVQTDEDSIDLPNDNGIEVGTEKSTKIGLPKTQNLP
                     MKDSRLNQFKENDQSFVHQSPAGTVQMKSSRLPENGNLTTVSYNLAESGETENASVQN
                     IVTKEAAPKPINKVHNGYRPANVAFTAPTKINVTRETKNRNNELKEQLLKDIKP"
     gene            13139..>13581
                     /locus_tag="IV250_002211"
     CDS             13139..>13581
                     /locus_tag="IV250_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769461.1"
                     /translation="MEDILIATFITALGAFLTIFVVTIKGFKDTDSLQKDHQSILDGQ
                     KTHTESIIVHLSEGFTYLKDAMPTFTVKLTEIFHSVEQISSELKEQRQQKDRQLGSLN
                     FRQETIQESLENVNRLNDEMQRVHLENEELRAQNYDLIQKKRNLE"
BASE COUNT         4837 a         2125 c         2654 g         3965 t
ORIGIN      
        1 ttaatttttt ctcaattatc aatttatcca agatatcaac gattgttcat tcattttcta
       61 aacattcaat gtgcttccat gccattagag gtgattaaaa agctcttgat caatccaatt
      121 tttatggtta gtttggttct tcttacagga ttggtaacgt gggtattagg aaaaaaagaa
      181 tgaaaagctt acgatcgttt aaaaaaatat tagatggagg gcattatgga aaataaaatt
      241 cattgcacta cgggaagagt aattacttta gtgttgtttt tgttccttct tctttttgcg
      301 aggggaactg cagtccaggc cttagaaaaa gttgatccga gtcaaatcaa agaaaataat
      361 aatctggtgc tttatcatga tagtgattca ttgagaaata tcgaagtaac tgggatcaat
      421 ggatttaaga tactagatga gtattcaact aaaaaacaaa aagtacttgc ttggccaaaa
      481 gtcaaagatt tgaaaagtac gtcaaaaatc gttgcccatt atcaaaaagt aggtacttat
      541 ctaggaaaag aaattgattg tgacgtgact ttttctaatt ttgtagcgca cgctggggtt
      601 aaccaagacg gattagctaa aacaattaat ggcaaaaagt attataatta tttgctcttt
      661 gataaaaata tttatcgagg atttttcatg cagtcaatca atgtcatgaa tgcaaaacta
      721 gaatttaagt atgcaggaac aaaaacaaaa gtagctttga gtgaaccaca gccagaaatt
      781 caatcaaaaa tgagatcgat gagagcagct ggtattgatt taaacgatac atttctatcc
      841 atttacagtt taaatggaca gtatcagcaa cgtgtgtctt ggtataatga caataatgaa
      901 tctgtcggtg aacgtaatat tgatatgaga gaatttgttg ggtatgaaaa aatgggtagc
      961 ttaccttatt ttgtcacaac agatacagca tgtgcagaat acaaagctcc tgcgttatca
     1021 acaaacaatt taacttcaaa agtagtggga ggacgtgcag aaaaggctta tagctcgaat
     1081 gatcatttca ccgatgttgt aggagctgat acttatcaca gaagtggtgt aacgtatacg
     1141 cttcaaggcg cttccccaac attcatgatt ggcgcaaata cgaatagtat gatgtttagc
     1201 tttgatactg cattgctatg gacaccacaa ccatcgaagc ctacaaaaga agtgtttaac
     1261 aaagctaata ctgaagaggc agcacacaat attgacaaaa aagtgattcc acaaggatca
     1321 gatgtttact atcatattca ccaaaagttt gatgcattaa cagtcaacac aatgaacaaa
     1381 tacaaatcat ttaaaatcac tgataccttt gacagcaaaa attttgatat ggtatcggat
     1441 gggaaaaact atgatggcgc attgtggtat gttgaagcga atggtacttg gaaaaaagct
     1501 gatacaggag gaaagttaac tgtatcaggt aatacagtca cttatactgc ttctgacagc
     1561 tttttagcta acgctgcaaa ttacggtagg gattatattt tgcagctcca tatgagagca
     1621 aaaaatgatg caaaaggaat tgcaccaaat gtggctactg taacgtttaa caatgatccg
     1681 aacaaaggaa cacaaaaaac aaatacagta gaaaactatt tctctcttaa accaaataaa
     1741 aaagtggaac aagatgggaa agatgttgat ggtcgaaatg atggtaaaaa aggaacgcct
     1801 acagctcctt taaatgcagg atctgaagta caatatctag tcacacaaaa atggcataca
     1861 aaaggtgtag acattgcctc agatcattac aagcaattta gtattcaaga tccagtggaa
     1921 gatcgtttga cgtataaaga aggttctgcg cgagtgatag atatgagtac aggaaaagat
     1981 attacttcag aaggtacact aacctatgat agcaattcaa gaacgctgaa gtgggaagct
     2041 tccgctgatt tcttaagtaa aaatctttta gatggacgag aaattcagct gatatttaca
     2101 gctaaaactc ctttgaatga agaattggat atcgacaacc aagcaaaagt ttcagtagaa
     2161 agtattgaga aagcaactaa tgtagtaacg attggggtaa aacctaactt acctcagatc
     2221 attgtgccaa aaactggatc gaatcattta gtgctaactg ttattggaag tatattattt
     2281 cttactttag catcattggg ctatgtagtt ttaaaactca aataaatata aaaacaaggc
     2341 tgagaatcag ccttgttttc ttttatttga agattggatt cgcactaata aatacattat
     2401 ctacgccaat tctaccagca gagtcaacta caaaattatg acctaatccg agaatacttt
     2461 ggttattata aagtaaattt gcattatggg cagggctatt tttccatcct accatggcat
     2521 cgatagcacc atcgtctgat aaaaggtcat caatatacag cccagtttga cttatgtttt
     2581 ctcccttagc accgtagcag cctaagcttt ccaataaatc ttggaaagta ccaccaccag
     2641 ggcgatagtg actgaagctg acttgtaatt ctttagctcg aatatctgca gcctgttgaa
     2701 caatatcatt gaattgcatt ggggcaacac cattttgttg gcgataatca tttaaccaag
     2761 caagttcttt ttgacgcata aagttacgta attcgtctaa tgaaatcttt ttatatttga
     2821 atgtgattgt tttattttga tcgacgttat taatggatcg ttggccgttt ttatccacgg
     2881 taacggtagg atcgtcattt ctgcacaaga aataattttg atcagtcata tttttccaag
     2941 gagtagagaa gctatcccct tcgtatactg tttttgattc actagagaga acagcgttgg
     3001 tatcagcatt tacatgatta acagtcacat taaatttacg agtgtagttg aaaatataat
     3061 tttgactttc agtcacattg tttaaggtta ctgaatttgt tggttctacc cgatcgtcta
     3121 aaacatatcc ttgaatagaa ggagcagtta aagtgacact ttcaccgtga gtgacgttga
     3181 ttatctgttc tttcaaaata tcaactcgcc cctcattttt gtagttgtta ttgcacaaca
     3241 aattgatttg atgcacttga ttataattga atgtgattgt tgtattacct gtgattgttt
     3301 tgctttgagt atcattacca atcactttgt agctaaaatt atctttagca ccttcgctat
     3361 atttaaatgc ttttgcctta gcagtgtaag tagaaccaga agtcactttt gcttgatctg
     3421 tagcaatcac ttgacctgtt tgattgtcaa tatggttaat ggtaaccata tattcttttg
     3481 gcgtttcagg tttgtgttgc aaccctttaa atgcagcatc aagactattt ttagcattgt
     3541 ttacttgtgt ttgagaaacc ttagaatcgt ctagtacttt tttagcattg gataaagcag
     3601 tctgaaaatt attccaagat tcttctgtaa aatctccttt agcagttgat tttactcggt
     3661 tgtataaagc ttgtaattca gctttattca cagtaggaac tggttttttc tttaatcctt
     3721 tgtaagcaga atctaattta tttttggcac tatccacttg cttttgagtc gcttttgagt
     3781 catttaacac tttcttagca ttgtttaaag cggtttggaa tgcgttccag gtattgtcgg
     3841 tatagtcacc ttttttcgtt cccttaacgc tcgtataaag agcttctaga gccgatttat
     3901 tcactttggg tttagaggtg tcagcgccat accaaccgat accttcgtat ttccagccat
     3961 agtctttggt taaccattgt acttctttcc aacttgcagt gtagtgatgt gagccagatt
     4021 ttacaccagg aaggaaaagg cggtagatcg gtttattatt tttttcggca gaacgccagg
     4081 caggcccttc ataagtccag ccgtaatttt tagtcaacca tttcacttca tcccaactag
     4141 ctgtgtaatg gtgatcacga agacctttat tgtacaaacg atagacaaga tggccaccgc
     4201 tagtcggtgc ttcccaggcg gggccttcat aatttcccca ttttatcttt actagatggt
     4261 cacgttcttt tttgctagag gtgtaaaaat gttcattatt atttggattg taaaggcgat
     4321 acatgacgtt agttgaagca gcgtcaacag atccagcaag actaagtgag gcagcaaatg
     4381 ttccaatagc taaaacagtt tttggattaa ataatttcat tttttcaact cctaaattta
     4441 attaaaaatg tttacatgat tattatatat cggctaaccg atatagtcaa taaaaaataa
     4501 gaaaagcctt atataatttt ggcttttctt attttatttg ttagttgaag tattaatatc
     4561 aacgatttct tcagccaatt tatttaattt catagcattt atgaatctca tgttgtctat
     4621 actagagcgt ttgttggcaa ggtcacttac tgtagttaag ggtacgccag attctatgga
     4681 tatagaacga actgatttac cactatcaaa cagcaaccat tcgattttct ttttatcagc
     4741 gtacatttca atgctccttc ttgttttttc tttattatag ctcttttttg taatgaatca
     4801 caatatcaag agaaaaattt tctatagaaa ggtggatttt tatgagtatt cgttctccgt
     4861 gtatccaaat gaggtttgtt gctactttcg ttatttgata tactaaaaat aaacaacgaa
     4921 agaaggaata aaatgaagaa aaagcaacta tggtttgtct ctatgtttgc aatatttttt
     4981 attattttaa gtggatgttc aagtaataag aaatcggaag ctaaagaaga taatagttta
     5041 ttgcaaccag gaactgtttg gaaagaagaa gtaggtggtc ttattcataa tttaaaaatt
     5101 atagatgata ctacctggga atattctgaa agtgtatggc atccagatcc tgtacaaata
     5161 acagttaaga ggcaaaaaga ctatgaaggt ttagaaagat acaaaatagt agattctgca
     5221 ggcattaggg aatttataaa caaacctgat tcattattta ttgttgttcc ctatgagaag
     5281 gatggcgtaa aaaaaataat ttttcttgga tctagcaagg atgagaagcg aacgaaagag
     5341 aaattaattc atgatggatc agagttaaat aaatacaaat tacaaaagac tagtgagtga
     5401 ttgctagtct ttttatttat cctgaaggag gaatcacagt ggcaaaagtt aaaacaccat
     5461 tcgaaaaagc cgcgtctaaa atcttagaac gccgtggtgt ttctgaaata cagtggaaaa
     5521 aagaagttct tgaaaaagca caatttgacc tattacgagg aaaagataag gacttagaag
     5581 aatatatcta tcaacgtgaa cgtgaaaaac ttgtattcga tgaaattatt aaacattaaa
     5641 aggagcgaat aatcttatgt ttgaatcaat tacaatgaat tttttaactc aaccgaaagt
     5701 agtagctgca gttgatattt ttagagcatt acaaactgcc gcaactagtt tgaaaaataa
     5761 tctgatgatt tttgctgctg ttgcggcagt ggcttccatt gctcttggag gtattttctt
     5821 cttctttggg cgaagaggag ctgaaaacgg gaagacacac gtaggttatg tattgttggg
     5881 ggtaggagtt attttagctg ccgcttctat tgtagtttca atgttctcaa tgtttggaca
     5941 aaccccacaa ggactataaa aaaaacaagt aggaggagta aaaagtacgt ggctactttt
     6001 tactcctttt tttatgaaag gagaatattc atgtttttta cgatttcagg aaatgcaaaa
     6061 ttggataaag agttaataag aaaagcaacg aagaaagtgg gaaagcgtac ttccttttta
     6121 ccgaaaacaa ttaaatattc tgtcattgaa acggaagaac agctacatgt gttaggacaa
     6181 gtgttagaac aagagaatca atcttctgtt aaattgactc tcacgttaca tgataaagaa
     6241 ggaaaaatag aaaaggctgg cacagaatac gtttttggcc cactggatta tcaaatttca
     6301 gaaggttttt atcctacatt gatagattta atccgccttg atgtgtttaa agatacggat
     6361 gaaactatga gtgtggaaga gtcttatcga aacgaaaaac ttttacaagt tttagaagaa
     6421 gagttagaga gcgaccaaag aaaagtgaat caagtgagtg aagtcgaagt aaatgaagaa
     6481 gtaccatggg aacgaccaat ctttccaaag aaggagtcaa aagaaaaaga gacagtctat
     6541 gttgacgaac aagctgatat tccgactcag aaatcgatat ttgaggaaga gaacgatgtg
     6601 atgggtcaat tggaggtaga tccctttgaa gaaacaatgg aggatgaaga gacggacgaa
     6661 gtagaagttg atccagttga agctaatgaa gagtcaacat ggactgaagg ggattttcag
     6721 gaatatattg aaccacttat cagtgaagaa gtagaaaaaa acgaaacaga gatacaaaag
     6781 gcaactgtat ttgattatat tttggatcaa tatccgccag agcatgaatg gatgaaagaa
     6841 aaaaacaaat tatttttaaa tgagctttat tcgaaatatc aattacccca aacctatcaa
     6901 gcatttttag agagtaaaga agaattaatc tcacagggta aaggcacact tttagaacag
     6961 ttagagtctg tccaatcaac aaattggcat gagcttgcac aagaagattt attagccacc
     7021 tttgaagaac gtcaacaaga aacagatcaa gaaattaatg agtatgcgac ggaacagtcg
     7081 tctaattggg agaaagtaaa agaggaacta aataaagaag aacagcgagt gattgaagaa
     7141 gaagtacaaa aagtcaaaga gcggtacgaa aagaaacgcg acaatgctta ttccttgtgt
     7201 gtagaaaaga tcaacaattt tactaagacg aatcaagaag cgttatcgaa agaaaaagaa
     7261 aacttgttaa aagaacgtat taaagaacga aaagaagagt tctaccgcgg tttacgtaca
     7321 gataagctaa agattagcaa agaattaaat gaacaactga ataagctata tcaaacaacg
     7381 actgaagttc ttatggaaaa acacgaacag atccagcaag aattacaagt acaaattcca
     7441 aaatggaaaa aagagtatga aattcaacaa caagaattga aagaacaaga agaaaaacaa
     7501 cggcaaaaag aaaaagagcg tgaacaatta gaaataagaa aacgggaaca ggatcttgcg
     7561 gaacaaaaaa tggcgataga aaaacaacgg ttagagcaag aaaaggaaaa agaagcagct
     7621 gctcgtaagg aacgtcaaga acaaatggat acattgcgtc aagcacagtt gaattatcta
     7681 gcacaacctc aaccagtagc tgctgttcaa acgccagtga acaaccaagc aaatttaact
     7741 ccttcacccc agcgttcctt taacggatgg atggtgggat gtattgcaag tttaagcttg
     7801 cttctaggtg gaggaacagt atttgctttt aatcatatca atcaatcttc acaggctgat
     7861 tctgtagcca caacggcgtc aatcaaggaa gaagcccagg aacaagcaag aaaagagtat
     7921 gaacaacaat tagaagaatt attgaatcgt accaatcagt caacaacaga gtcaagcatg
     7981 gaaaatagca aagagagtga ttctagtaag gctaaagaag gatcaagcga ttcaactgta
     8041 caatcaaaca cttccgaaag ttcagaatca aatgaggtga aataatgcgt gttagagaaa
     8101 caacgaagcg aaaagacaaa cgattcaatt tagaaaagaa taggtatctt tctcatttct
     8161 atcgctatac tccctcagga gaagagctac cagaaaaaca tgacaaacgc atttcgaaaa
     8221 agatttttta tagtggactt gtatacctcc ctttagagta tttagtaaca agagtaccag
     8281 aagttttcaa ccgattggaa attgttaagg gaagcttaca aggaataaat ctgtttcaat
     8341 tacaggaaat gccacaggtc tttcattttg attcagctgg tttttggaat ccatttactc
     8401 ctacaactag tttgggtaca cgtttagcaa tttcgacagt ttgtgtggtt ccagctgtac
     8461 ttatgggatt gaaaatggac atgtggtttc gtcctctagc agatggacaa aaaggggata
     8521 atcgattaat gaccttaaaa gaaatccaac agatttatcc gcaaatacct gaaacaaaaa
     8581 ctacttttcc aggttatgga ggtattccaa tcacacatta caaaaaatat tggtacattc
     8641 aaacagatac tgttaatacg tgtattgttg gtacctctcg atcagggaaa ggtcagattg
     8701 aagtgttagc caccattgac aatttatctc gtgcagaaaa acaaagttca atggtcgtta
     8761 atgatccaaa aacagaatta tatgttggta gtaaagatga actagaagct agagggtatg
     8821 atgtatatgc ctttaatatc accgatccac tgcaatcaat gagtgataat ccattggcac
     8881 tcattgtgaa atactggaag cgaggagaca tagacaccgc aacacagtta accaatactt
     8941 ttacgaatac gatttattat gatgcaaatg cttcagacaa taaatacttt aacgaaaatg
     9001 cgcagaaagc tgtcaatgcg cttatttttg cgttattgga acaagcggat caaacagggg
     9061 attactccaa attaacacca aataatctcg ttgaactact ttcagaaatt ggcggtttta
     9121 attattatcc agatcctgag aatgaattca agcagctcaa tgccttagat gagttcatga
     9181 atcagttacc ccctggaaat atagcaaaaa aacaatatgg ggcaacaaaa attggatctg
     9241 ataaagcgaa agggaatatc ttgtctacgg caattacagg attgaatcca tttaccctaa
     9301 cgaaaattgc gaagatgact tctcaatcga ccattgatta caagtccatt ggttttccga
     9361 agtatttgga tctcaaatta catgaaagcc tattaaatca acgcattgtc gtggaatttt
     9421 atcataaggg aaagaaattt cacgaggaac gagtcaaagt tggctatcgt ggtttttgtg
     9481 aaatcaactt tgattgtcat ttagagcagg gagatcatgt ttgcttaaaa tacaatcatc
     9541 aaggaaaaca atatgttgct tggtatgagt tttctcaaaa agtattgaaa gacgaagagg
     9601 gaaaaatcgt ttatcgaaaa aaacgaggag aaactcattt gccagaacta gaaaaagaag
     9661 ccgttctaac attagatgag aagaaaagta acctattagt agagtctatt cggatgcact
     9721 ataccgataa accaatggct atttttatgt taacgcctga ttatgatcca tctaaccata
     9781 tcattgttag catctttctt agtcagttgt ataaagaatt atctacgcaa tgtgttaaga
     9841 ctaaaggaga taaatgtcac cgtaggattc attttatttt agatgaattt ggaaatatgc
     9901 caccgatgga caacatggaa ggaatcatga cggttacggc aggtcgtaat atgttatggg
     9961 atctgtttat tcaatcttat cagcaacttt atgcgaagta tggagaacaa aacgcagcga
    10021 ttatcaaaga aaattgtcag acacatattt atattatgtc gacagatgat aataccatcg
    10081 aagaattttc taaaaaagtg gggaataaga cggttgaaca agagaacagt accatcaatt
    10141 cattaggtat gaatactcat atcaatcgca atgttgattc agatcgtatc ttaactcctg
    10201 aaagaatttc tactttttta gaaggtgaaa cgattgtact cgctccttta aaacgacaag
    10261 atttgaaagg tcataaaata cggccgtatc ctatttttaa tcatggggaa acgaatcttc
    10321 cttatgctta tcaatatatt gaagatttca atccatcgtg ggatatcaat gatatagaca
    10381 tacattgtac acatgcggac ttggatctta aggctttaca aattgattat gattatttcc
    10441 taccagatga atatgctaga aatctctatc atggactgca tgatcaatta gaagaaaaaa
    10501 acgaagaaaa tatgttttat aaaatcgttc aatcttttga gaatgagacg gctaaaaaat
    10561 tgttaattca attttacgaa gaacaagcaa cggatcgtct tcaagaatat gttgaaaagt
    10621 tgatcaaaaa tgaagaaatt tctgaaatga ttaggaaacg gctgttattg gaagctgaaa
    10681 aggcggtgaa agaatgatca ttggaggaat tttctctggc atagccgact ggtggtcagg
    10741 tttattcgga gaaggggatg cagatcccaa aaccgtaacg aaagtcttac aagattatgg
    10801 taattatctg cagtatcaag atgtattcag ttggatttgt aatacttttt tatggggatt
    10861 gatcaaaggg ttatataatc tcaatacttt attagaaaaa acaatttatc aaagctttga
    10921 tttaaaagac ttattgaacg ccgctggtgt taatgaacta tttcaaagtt tgattagtaa
    10981 ggtattggct cttttttgtg tcgtcacatt aatttattta ggcttgaaat tttttatgag
    11041 taaacaccca ccgaaagtca aaaatatctt ggttaattta tttatggcca tgatcttgat
    11101 ttttggtggt agttctttaa tcgatgaagg gttaaatatc agtaaaagct tttacggaga
    11161 cgtcactgca gcaaataaag cagataaaac gggatcacct gcttttcaaa ttattcaaaa
    11221 taatgtgatt gatgtcagta cattactagc gactgacccg aataaagtag aatccttacc
    11281 aacagataaa agaaatgctt taacagcgaa aaatttcaaa acagcgaata tcaatgggat
    11341 tattacacct gatcaagcta aaggtatggc aggtaatgat aaaagtgggt tatcagatat
    11401 acaaaaggat cggttaaagg cattacaata ccgattggaa ctagatgata atgggaaaga
    11461 aattcctgtt caaattagtg acgaaggctt agcaaaatat gtgtatacgt caggctatcg
    11521 tcgatacatt agtagtccag ggattatttt ggctggggaa tcttctttgt cggtagccta
    11581 tttatttatt ttgtttacaa tcgttacttg cattattgag ctagtattta aaaaattcta
    11641 tttagtaatt gccgcgtcaa cggactttga gacaggacaa cggatgaaaa gtgcgataga
    11701 agatatttct caatccttct tgttattggc gtttacgatt ttagaattac gaatctatac
    11761 attaatgctt tcaggtatcg gtgatttgca tgcggtagga aagatcaatg gattcttgta
    11821 tgttgttgct ttaattgtgc taactattgc gctattcaaa ggctctcaat cggttacgaa
    11881 gattttcggt gttgatacga gtctgaaaaa cggcagcaat tctcttctta gcatgtttgc
    11941 cttaggaaat attgcgaaaa atgtgggtag cggagctaaa aaccttgcaa gtgctggtaa
    12001 gagtggttta agccatttaa atgatattcg taagaatgga tttggccatc gaaaagatat
    12061 tacccatgat aacggggaag aacaatcaga cgataaagct acaactgtac ctaatagtaa
    12121 gaagacagtt tccgaaatgt tcgggaaagc gaaaaatggt gtttctaaag ccgcagaagg
    12181 tcttggttac gtcaatgaac gtgggttaaa aggaacagtt gaagatctta cagaaaaagc
    12241 caaagacacc ttagaagaga aattagacgg cacgagagct aaagaatttg ctgaagctgt
    12301 gaagaatcca tccgaagcac tagaaaaaca aaaagaaaaa gcttcagcag tcaaagaagg
    12361 actaaaagat gcgtatggtg aaggacaagt caatgcagca gtgaaggcaa atacaacaag
    12421 tgaaaaagaa aatcaagaag gtgcagcgat tccttcagcc aaagatcacc aagatgagtt
    12481 acattctaac gaaaattcga tcggagaaaa agattcagaa gaaggacaac atggtcagtt
    12541 accaaaaacc gatccagaac aatatcgtta taaatctaac attaattctg aaaacgaaaa
    12601 acgcatttca gatttaccaa aacgaaatca agaagtacaa acggatgagg attccatcga
    12661 tctgccaaat gataatggca tcgaagttgg aactgaaaaa tcaacgaaaa ttggtttgcc
    12721 taagacacaa aatttaccaa tgaaagatag tcgtttgaat caattcaaag agaatgatca
    12781 aagctttgtt catcaatcac ctgcaggtac tgttcaaatg aaatcaagtc gtttgcctga
    12841 aaatggaaat ctgacaactg tttcttacaa tttagctgaa agtggtgaaa cagagaatgc
    12901 gtccgttcaa aatattgtga caaaagaagc tgcacctaag ccaatcaata aagttcataa
    12961 tggatacaga ccagcaaacg tagcatttac tgcaccgaca aaaataaatg tgacaagaga
    13021 aactaaaaac aggaacaatg aattgaaaga acaactattg aaggatatca aaccataaaa
    13081 ggcctattta agggcctttt tctttataaa acaagtttaa aatggaaagg agaaaattgt
    13141 ggaggatatt ttaatagcta cgtttataac tgcactggga gcatttttaa cgatatttgt
    13201 ggtgactatt aaaggattta aagatacgga tagtttacaa aaagatcatc agtctatttt
    13261 agatggtcaa aaaacacata ccgaaagcat tattgtacac ctttcagaag gattcactta
    13321 tttgaaagat gctatgccca cattcacagt aaaattaacg gaaatatttc attctgttga
    13381 gcaaatatcg tcagaattga aagagcaaag acaacaaaaa gatcgtcaac ttggctcact
    13441 taattttagg caagaaacga tacaagaatc attggagaat gtaaatcgat taaacgatga
    13501 aatgcaacga gttcatttgg aaaatgaaga attacgcgca caaaattatg atttaattca
    13561 aaagaaaaga aatctggaac a
//
LOCUS       DACDIE010000063        13309 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.063, whole genome shotgun sequence.
ACCESSION   DACDIE010000063 DACDIE010000000
VERSION     DACDIE010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.063"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            274..2013
                     /gene="yfcC"
                     /locus_tag="IV250_002212"
     CDS             274..2013
                     /gene="yfcC"
                     /locus_tag="IV250_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAP8769462.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2170..3354
                     /gene="iadA"
                     /locus_tag="IV250_002213"
     CDS             2170..3354
                     /gene="iadA"
                     /locus_tag="IV250_002213"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAP8769463.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3465..3668
                     /locus_tag="IV250_002214"
     CDS             3465..3668
                     /locus_tag="IV250_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP8769464.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3769..4431)
                     /locus_tag="IV250_002215"
     CDS             complement(3769..4431)
                     /locus_tag="IV250_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8769465.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4444..5946)
                     /locus_tag="IV250_002216"
     CDS             complement(4444..5946)
                     /locus_tag="IV250_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8769466.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVTVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6494..8173
                     /locus_tag="IV250_002217"
     CDS             6494..8173
                     /locus_tag="IV250_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP8769467.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8300..8779
                     /locus_tag="IV250_002218"
     CDS             8300..8779
                     /locus_tag="IV250_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HAP8769468.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8998..9678
                     /locus_tag="IV250_002219"
     CDS             8998..9678
                     /locus_tag="IV250_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAP8769469.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9686..11017
                     /locus_tag="IV250_002220"
     CDS             9686..11017
                     /locus_tag="IV250_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP8769470.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            11032..11994
                     /locus_tag="IV250_002221"
     CDS             11032..11994
                     /locus_tag="IV250_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAP8769471.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            12105..12443
                     /locus_tag="IV250_002222"
     CDS             12105..12443
                     /locus_tag="IV250_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAP8769472.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12522..13229
                     /locus_tag="IV250_002223"
     CDS             12522..13229
                     /locus_tag="IV250_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAP8769473.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         4270 a         2331 c         2904 g         3804 t
ORIGIN      
        1 atttttcaat gattgtaaaa tgaaaaaaac aggacgagat tttctctaat ctaaaaataa
       61 aaaaagaatt ttaaggaaaa acagagaaat aaaatactta atataaatgg aaagagaaaa
      121 aaataatcat acataggaga aaaaaaacaa aatagaaaaa agtgattttc taataaatta
      181 ttcatgtgga aatgtcattt ttactgtata attcatttga ttatccattt tttattcatc
      241 aaaattactg aaaaaagaaa ggaagaaata aatttgaaga aaacaaattc ttcaggaaaa
      301 ccgaaacgcg acttcactaa attatcgaca ccccatacgt atgtcattat ttttggtgta
      361 gtcatctttg catggatact gacttttgtc gttccagcag gaaagttcag cacgcaagat
      421 attgaataca aagatgcaaa tggcgaaaca agtactcgta ctgttttacg tcaagattcc
      481 ttccgttatg cttacgagct ggataagtcg tatgtgtttg atcaattaga agaactgcaa
      541 gatcatccag cagaacgtga aaaactggat gttccggaaa aaggattgga aaaagtcatt
      601 gctgacggag aaaaaaatct tacgcaggaa aaactagatg aaatctcctt gacagatgat
      661 gtgctttacg atcagtacgg cgaaaatatc tatgatactt ctaaaaaatt gcataaaaca
      721 gccaaaatat ggggaacaga tgattttggc ggctttggtt tcttaaattt tgtttttgaa
      781 ggactagtat ctggagataa gtatggatct gctgtaggga tcgctgcatt gatccttgtt
      841 gttggaggtg cttttggtat tattatgcgt accggagcga tcgacgcagg tatctatgcg
      901 tttatcagta aaactaaggg actggagcgt ttagctttac cgctattatt ctttgctttt
      961 tcattcggtg gcgctacatt cgggatggca gaagaggtaa ttccattctc aatggtgatg
     1021 gttccatttg tcattgcgtt aggatatgac tcgatcgtag cagtaaccgt gacttatgtc
     1081 gcatctcaag tcggaaatgc gacctcttgg atgagcccgt ttagcgtagc ggtggctcaa
     1141 gggattgctg gaatccccgt gttgtctggt gcgacattcc gattgattat gtgggtagtt
     1201 gttactgcat tagccgctgg ttatatgatg atatatgcag aaaaaatccg taaaaaacca
     1261 gaaagttcgt tgacttacaa atcagatgat tatttccgtt ctcatatcaa aaagacatct
     1321 gatgaaaata aaaaattcat gcttgggcat aaattgatct tattagagat gttagttgtc
     1381 cttgtctgga tcgtttgggg cgtgactcaa aaaggctatt acattccaga aatcgcttct
     1441 caattctttg tgatgggatt agttgctgga atcattgctg ttcttttcaa attagatgga
     1501 atgaaagtca atgacatcgc ttcttctttc caatctggtg cagctgattt agctggtaca
     1561 gcaatcgtag taggtatggc caaagggatt cttcttgtct tgggaggatc tgatgcatct
     1621 gttccttcag ctttgaatac gattttgcat gggatcggga cggcgttgac cggtgttcca
     1681 gctgtcattg gtgcttgggc gatgtacatc ttccaaagtt tgttcaactt ggttgttaca
     1741 tcaaattcag gacaagcagc tttgactatg ccaatcatgg ctccactggc agatttagta
     1801 ggtgtatcca gacaagtagc agttcttgct tatcagcttg gttcaggttt tatggacgct
     1861 tttacacccg tatcagccag cttgattggt gtgctaggtg ttgcacggat cgaatgggca
     1921 aaatgggcaa gattccaaat caagatgcaa ggattcctgt ttgttttagg aactattttc
     1981 atcatgattg ccattgcaat aggtctgcag tagtactgga gacctgtaaa taaaaaagta
     2041 atccaaacag tagataagga ctccaagaac cgtcatttta cggtaaaatc aaaggagtaa
     2101 agatcctatc gtttggatta ttttttaaga tcctatcatc agttatactt aaagaaagag
     2161 gaaaaatgta tgaaaatcat tcgacaaatt gaagtgtttg caccagatta cttaggaaaa
     2221 atggatgtac tgattgccgg tgggaagatt ttggctgtag aagaacagct agaaggcggc
     2281 tacgaaggag tggaagtaga agaactttca ggtgagggaa aagtactaac cccgggtttc
     2341 attgactgtc atttccatat tttaggtgga ggaggagaag gcggttatca aaatcgaacg
     2401 ccagaagtca cattgagcca attgactaca gctggtgtaa cgacagttgt cggttgtttg
     2461 ggaacagacg gagaaggtag agatatgact gcgttgatca gcaaagcgaa aggtctggaa
     2521 gcagaaggaa tctccactta tgtatacgaa ggttcgtacc gattgccggt aaaaacagta
     2581 acagattcta tcatcaaaga ctttttgaca attgataaaa ttatcggaat cggagaaatc
     2641 gcagtatccg atcaccgttc ttctcagcca agttttgaag agttcgctcg tgcagtagca
     2701 gatgctcgtg taggaggcat gttatccgga aaagctggga tcatcaatgt ccacttaggc
     2761 ggaggaaaaa gaaagctaga gctattgaca cgaatcgtag aagaaacaga aattccgatc
     2821 acacaatttt tgcctactca tgcaaatcgc acaccagaat tatttgaagc ttgcgtggca
     2881 tttgccaaac gtggtggcac gattgacttc actgctagcg aagatcctga tttttgggag
     2941 aagacagatg gtgaagttcg cttcagcaaa gcactgaaac gattgatcga ggaagatgtt
     3001 tctttagata atttcacgat gacctcagat ggacaaggaa gcttgcctta ttttgatgaa
     3061 aacaatcatt tcctagggtt aggagtagga agtgccaagg cattgctagt agggatcaaa
     3121 gaagctgtgc aaaaagaaag tatccctttg gaaatcgcct tacgagctat aaccagtaat
     3181 ccagcacgta tattgaaatt ggataaaaaa ggaaaaatcg aaattggtgc tgatgcagat
     3241 ctgtgtatct tggataaaga aacgcttgat attgatacag ttatcgctaa aggagaaata
     3301 atggtccaag aaaaagaagt caaagtatgg gggactttcg aaaaaagttt ttaatcaaca
     3361 gctctaacta tcttttttag aaaacgagcg ttctgtttgt ctgtatctgt tgttgatgtt
     3421 accctgaagg tagatcaata aacaaactaa gaggaggaag cacaatggca gatttaaaag
     3481 gacgttctaa agatatgaaa gataaagtag tcggtgaagg aaaagaagca tacggcaaag
     3541 caacagacga caagagcaaa gaaacagaag ggaaagcaca atcactaggt gctgacatca
     3601 aagaaaaagc tcgaaattta ggcgacgaag tcaaagacaa gttttctcga aaagacgaaa
     3661 aaaaataaaa accacttatt actaattgaa tcaagcaaat aaaaaatccg aaccatatct
     3721 gcgagacaga tatggttcgg attttttatt tgatcattat cctgtttttt aaacttcgat
     3781 tttttgaaat tttttatttt ttagttcaaa gacttcatca cagctttttg ctacttcaat
     3841 ctcatgagtc acgatgataa tacagcgatt ttgttcatgg gcgatttctt ggaaaagttt
     3901 gacgatatct tgtgaagttg tttcatctag atttccagta ggctcatccg ctacgattag
     3961 ttgatgttca cagcagatag cacgaacgat agcgactcgc tgctgctgac ctcccgaaag
     4021 tttggtcacc tgtttttttg ctaatatttc atcaattcct acacgagcca gtcgatctaa
     4081 agcaacttgt cgataattct cttgcttggt cttagtgatt tccattgctg ctatgacgtt
     4141 gtctagagcc gataaataag gaagcaagtt gtaagcttgg aaaataatcg agacatcatt
     4201 tttacgataa cttcttaacc cgattttgtt cagtgaacga tcttgatagt atacctttcc
     4261 ttcttttggt gcatctagtc cagaaataag cgaaagaaaa gtcgtttttc cagaaccact
     4321 tgctcctaaa atagcgtaca tttttccagc ttcgaaattc agttctacat ctttataaag
     4381 cgcatcttgc ggatcttgat accaaaatcc caattgttct gtccgtaaca tcaaattcct
     4441 cctttagttt aataggattt ttcttgggtt taatcttaat atccctatag aagctaaaaa
     4501 gacagaaaac agactgattc caaatgctac tccggctaat agtcccaatt gtgcaggttt
     4561 gacattgatt tccaattcgc tgacttgatc agaaacagca aatggattat tcgtggttcc
     4621 ttgttgacca tcgcgttgac cgccttgttc ttgtctgtta cctggcattt gtccaccgcc
     4681 aggagcttgc tggttttgtg tcgtggtttc tgtctgttgg tcaagtagct gctgtcccac
     4741 ggcattcgcg acgacgtttc cgctaagcga agcaactcct aatgcaagaa tcatacaaat
     4801 agccacttcg gtaaacaatt ggagaatgat tttacttctg gattctccta aagatagcaa
     4861 gacgcctaac tcatgccgac gctcccgaat ggtaatcata atgatcagag taagtatgat
     4921 aataccggct acagtcacta gtaaaacaac atttttagaa aaacttgcca cgttgtttaa
     4981 aggttccaac attgactgat acatggaatc atttgattgt aagctaaatg tatctgtgtc
     5041 gatcagcttc tcagcttctt tgagaaactc atccatttta tctggatcag atagagtgta
     5101 aatagcagaa tctatcgtgt tatcgtcaga tgtcccattt agttcatttg caaaagtgta
     5161 agaagtgtaa agtgtattcg acggattcat gaagttgaat ttcataccca tactgccgct
     5221 tgtttcgctg gattcataaa ttcctttgat cgtcatttca taagtggtat cttcatcttc
     5281 agtactcgtg attgtgaaag tatctccgac tgccaggtca tttgtttctg ctagagtaga
     5341 atcaatgact acattgtttg tgcctttgtc gctgtcatct attccttcac catccgtgat
     5401 ttttgctgtt ccatcagaaa aattactatt ttgtgcagtt gctgacacac cactgatttg
     5461 aaagtctgct tgagtcattt gcggattccc gcccattcct ccagcaggtt caccgcctgc
     5521 atcagtagtt tctgaactgt cttcacttga atcagaacta gtgatggcag ttattccatc
     5581 ttttgccata gctgaggcgg aactttcaaa ggaataactc tttacattat ctaattcagc
     5641 tattttttcc gcgtccgtta aggaaactgg tgtaagagaa aaactgcctg gatctggacg
     5701 ttcttcagtg gtagaaccgc ttgaagaagt ttgttcctgt tttttaaatg aagcttctcg
     5761 attggcactt aaagtcactg ttgctccaac ttctttttgt gcttgattcg ctgcttcttc
     5821 tgcagcgctt cgaatcgtga ggccagctag cacaaaaata agaatggctg tgaacacggc
     5881 aaacaataac aatgaacgtc cccattttac ttttgtactt ttccatgcac gtttcataaa
     5941 attcatattt tgcatccctc ctagtagatg catttgataa tagacaacct atcttaaagc
     6001 tagcttaaat cttatgctgt ttgcacacca tcaacatgag gttctgtata tataaaaagt
     6061 cactatagtt attgcaaaaa atgtggtaaa atgaaaaaag tatgtttcaa accgacgaag
     6121 aagaaatgaa agaggaatag caaaagaaat cctagatgct acagagggat ttttacgtgc
     6181 atagttgttc taacaattgc tgatactagc gatttagttt atttgttgta taataatggg
     6241 gaacagctga atacttatat aagtattctg gctatagcgt ttcgcaaaag atccattttg
     6301 ttaatgaagt atatccaaaa taaaatgccc atccaactgt cagaacgcgg atgtgctggt
     6361 aattttcctg ctgattctcc ataagttgag aaatagtgat tttgcatatg cttcattgga
     6421 atggctcata tatttagata aagaagaaaa tcgacatctt taaacttgga tgtcataaga
     6481 agaggtgaag tttgtgagta ccattaaaat tatcccatta ggcggcgttc gtgaaaatgg
     6541 aaaaaacatg tatgtcgcag aagtgggcga ggatattttt attttagatt gtgggctaaa
     6601 atatcctgaa aatgagttgt tggggatcga cgtcgtaatc cctgatttta cgtatttaga
     6661 agaaaacatt gatcgtgtag ctggtgtctt tttaacacat ggacatgcag atgcgatcgg
     6721 ggcacttcct tacttgttgt caaaaatttc tgtaccagta ttcgggacag agttgacgat
     6781 cgagttagca aaattgaatg tcagtcgtaa tgaagcagcc aaaggattta aggatttcca
     6841 tgtggtagat gagcatacag aaatagactt tggtgaaaca gtagtgagct ttttccgtac
     6901 cactcatacg ataccagatt cagtggggat caatctgaaa acaaaagaag gtagtatcgt
     6961 ttatacggga gattttaaat ttgaccaaac ggctatccca atgtatcaaa cagattatgc
     7021 acgattggcg gaaatcggca aagaaggagt cttagcctta ctaagtgaat cggcaaatgc
     7081 agaaaatcct cagcctgtag cttctgaagc ccaaatagca gatgaagtat ttgatacgat
     7141 caaatattgg gaagggcgta tcatcgtcgc ttgtgtagca agtaatttgc aacgagtgca
     7201 acaagtattg gatgcagcag aaaaagcaca acgaaaagtt gtcttgactg gtcaagattt
     7261 cgaacgaatt attcgcacag cgatgcgttt agaaaaactt caattaccaa gtgaagatct
     7321 gcttgttacg ttgaaagaca tgaaaaacta tgatccagaa gaactgatca ttttagaaac
     7381 tggccgaatg ggtgaaccca tcaagtctct gcaaaaaatg gcaaacggta cacaccgtaa
     7441 tgtcaagatt gaagaaggag atttggttta tatcactact acgccaagca tcgcgatgga
     7501 aaccatcgtg gcgaaaacgg aagatatcat ttatcgagct ggtggaacag tgaagttgat
     7561 ctctgagaat atgcgcgttt ctggccatgc caatcctaat gacttgcaat taatgatcaa
     7621 tttgatgaaa ccaacatatt ttgtgccagt tcaaggagag tatcgtgaat tagctgctca
     7681 tgcagatttg gctcatgcag tagggatgcc atacaaaaat atctatatta ctggacgcgg
     7741 agacgtgctg gaatataaga acaagcgtat gagtgttgca ggaactacaa gtgcagaaaa
     7801 tgtaatgatt gatggattag gcataggtga tatcggaaac attgttttac gtgatcgaaa
     7861 aatcttgtca gaagacggga ttttcgtagc ggttgtcact atcagtcggc gagaaaagaa
     7921 aatcatttca aaacctcaaa ttacttcacg gggattcgtt tacgtaaaag ctagccgtga
     7981 tctgatgaaa gaaagcagca atatgattga aacaatcgtt gagaaacatt tagaaagcaa
     8041 tgattttgag tggagcaaat taaagcaaga catccgagat caattaggcc gttacttatt
     8101 tgaacaaaca aaacgccgcc cagtgatctt gccagtcatt atggaagcta ctcaacgtcg
     8161 aaataaaaaa taaacaaaaa gagtaagatg aacctctcta tagagtgctc gtcttgctct
     8221 ttttttgcga aaaacaaata atttttgaat acagcatccc tcgctttata ataaacgata
     8281 gaataaaggg agtgaatcaa tgatgagtca aacaaaagtc gaacgttatt tagaagaaca
     8341 aaagatcagc tatcaaccac ttgattttag tcatgtggta gaagaatctt tcacagaaga
     8401 actacaaaaa agagggattg atccagcttt gatatgtaaa actctcgtag tgaaaggaaa
     8461 caaaactggg gtgatcatcg cattgattcc tttgaacgat cacttagatt acaaaaaaac
     8521 gagacaagtg acgaaagatc gtaaagttgg ttttcctgga atggattttg tattaatgca
     8581 tacaggatat ccccacggag caaacacccc tattggaata aaactcgcac atcctgatta
     8641 ttgtttttta gcagacagtt ctttaaaaga aaaagaagaa gtaattgtgt caagcggtga
     8701 aatcgggaga agccttcgga taaaagtagc cgatttagaa aagatcgtcc atcctattta
     8761 cgctgattta attagttaag ggacgagaag agaagctaag cagagcagag acctcaaact
     8821 tgagattata tctgcttggt acttggtgca tcctgtatta tctgttacaa ttgctttgta
     8881 catactgtac tatcaatcca atgaaaatat atttagcagc tatatttttt cattgatgtg
     8941 caagtgatga aatcgttttt aataaaaaag atacagagaa aacgcgaggt gtaaaatatg
     9001 aaaattacgt atcatggaca ttcctgttta acaattgaaa tggaaaatgg ccaacgcgtg
     9061 atgattgacc cgtttattac aggaaatcca ttgacagact taactgcaga agaagtaaag
     9121 acagattggt tgctgattac tcatggtcat agtgaccaca taggagatat gattcctatt
     9181 gcaaaagaaa atcaagccac aattatcagt attgttgaga ttgctgatta tgcgaacagc
     9241 agaggagtag attcattcgg tatgaatatc ggtggaaaac atgcgtttcc attcggcgtg
     9301 gtaaaattcg tacatgcaca gcatagttca agctatgaag tagacggtat cgttcagtat
     9361 atgggcgagc ctagcggaat tattattcaa gcagaaggaa aaacgattta tcatgcaggg
     9421 gatactgctt atttcagtga tctaggattg cttgcagaag aattcgatat tgatgtcgct
     9481 ttcttgccga ttggcgataa ctatacgatg gggccagaag atgcagcaaa ggctgcgcaa
     9541 acaattcgtg ccaagaaagt agttccaatc cattacaata ctttccctgt cattgaacaa
     9601 gatcccgaat cttttgtaga gttactggaa gatagtgaag gaaaaataat gagcgtctcg
     9661 gaaacgctgg aagtgtaggc agaaaatggc gacaaaacat gatttgattt tagaatatat
     9721 tgaaagttta cctgttggca atcgaatttc tgtaagaagc atcgcaaaag aattgaatgt
     9781 cagtgaagga acagcgtacc gtgcaatcaa agatgcagaa aatgtcggac tagtttcaac
     9841 aattcaacga gtagggacca tccgtatcga acgcaaatta aaaaaacata ttgaacgttt
     9901 gacatttggc gaggtcgtca ggatcattga aggggacgtt ttaggcgggg cggcaggatt
     9961 agacaaggtc ttgaataaat tcgtcatcgg cgcgatgact aaacatgcca tggaacgcta
    10021 tattacgcca ggatcactaa tgatcgtcgg caaccgggcg gaagtccaaa aattagcatt
    10081 ggaagatggc gccgcagtat tgatcacagg aggattcaat acgactgctg agattgcaaa
    10141 gctagctgat gatcttgaaa tgccgatctt acgcacctct tatgatacat ttacagtcgc
    10201 aaccatgatc aaccgagcgt taagcgacca attgatcaaa aaagatatca tgcttgtcag
    10261 tgatatctat atgccagctg aaaagactca ttatttgcag cagacagata cgatttctga
    10321 ttatcagcgt ttgtcagaag aaacgcagca ttcgcgtttt ccagtcgtca accgccatca
    10381 tcgtttgatg gggattgtta cggcaaaaga tgttttaggg aaatcgccaa accagatcat
    10441 cgatcgggtg atgacgaaag aaccaatcag tgtaaagaaa acaatgagta ttgcctctgt
    10501 tagccatcaa atgatctggg atgggctaga agtcatgccg gtcgtttccg atgacttgac
    10561 attggaaggc ttgattaccc ggcaagatgt aatgaaagcg atgcagcttg tccagcgaca
    10621 accgcagatt gcagatacga tttcggatca gatatctgga aatatccata cgattgatac
    10681 tgaccgtgaa ggaaatcgtt tggaaaaccc taagttcaaa ttcgtagtag caccacaaat
    10741 ggtcaatggc gttggaacca tttcttttgg cgtacttagc gaaatcattt ctgatgtcgc
    10801 acaaaaaaca atggtgatga accaaaaacg aaatatcttg attgaacaag tgaacctaca
    10861 ttatttgcgg ctgatccaat tggaaagcga attagacatt cgtccacgtg tattggaaat
    10921 cggcagacgt tccgctaaac tggatatcga agtgtttatt gaaaacacca tcgtttccaa
    10981 agcaatcgtt gtttgtcaag taatggaacg ttcttagaaa gcaggagtga aatgacagta
    11041 cagcaagaaa tagtagatac aataaaaaaa tacgataaaa tcattatcca caggcatttg
    11101 cgccctgatc ctgatgcatt aggatcacaa gtaggattag ccgaactatt acgggaaagt
    11161 ttcccagaaa aagaaatacg tcaagtaggt gaaacgattg aagggcttcg ctttttagca
    11221 gaaatgcaag aagttgaaga tgtcttctac caaggagcgc tagtcatcgt gactgataca
    11281 gctaattcac ccagaatcag tgatcaaaga tatcagttgg cagaaaaact gatcaaaatc
    11341 gaccatcatc ccaatgatga cccttatgga gatcttgtgt gggtaaatac aaaagcaagt
    11401 agctgtagtg agatgattgc agaatttgct ttgatgtttc ctgaatcttt aaagatgaac
    11461 gttaaggctg cacggttact ttatgcagga atcgtcggag atacagggcg ctttttgtat
    11521 ccctccacga cgtcccgtac attagagatt gcttcgatat tgagaaatta tccatttgat
    11581 gcaagtgctt tgaacagaga gattgaacaa atgccgatga aagtcgctaa actttccggt
    11641 tatctttatc aaaatatcca ggtggatgaa aatggtgcag gaagaataat ccttccgcaa
    11701 gaaattcttg ataaatatgg aatcgatgat tcagagacag cttcgatcgt ttcacttcct
    11761 ggagtgattg acgaagtgct ggcttggggg atttttgttc agcagccttc agggtattac
    11821 cgcgtacgtt tacgttctaa ggggccgatc atcaatgaat tggctaaacg ccatcatgga
    11881 ggaggccatc cattagcaag tggtgcgaat gcaaaagatt tagcagaagt agaggaaatc
    11941 tataaagaga tccaagatat atgccaatcg tataagagca gaaaaacaga atgatacttc
    12001 gttcgaaaac gtgccttttg tagtaaaaat cacaaatgta tcttttcaag atcttctttt
    12061 tccgttacaa tagtgctcac taaagatttt aaaggagaaa gaagatgaca ttaccaaatt
    12121 gcccagaatg tggctcagag tacacatatg aagatcgagg cttgttgatt tgcccagaat
    12181 gcgggaacga atggaacaaa gaagaacaag aaactgtcat agaagacgga ttagtggtat
    12241 tagatgcaaa tggaaaccgt ctgttagagg gcgatagcgt gacagtgatt aaagacttga
    12301 aagtaaaagg tgcgtcaggt gccattaaac aaggaacaaa agtcaagaac atccgtttag
    12361 tagaaggcga tcataatatc gattgcaagg tagaaggatt tggaccgatg aaactgaaat
    12421 cggaatttgt gaaaaagaat taaagaatat tcaaggtctt ttcacattct tttcctacta
    12481 ctaatgataa aataaagtaa acgaaaagga gtgaaacgaa aatgtatggt tatggaatgt
    12541 atggtttttt agatccgaca tttcttttgg tcattcttgg attagttatt tccggaattg
    12601 cgtcagcgta cgtcaatagt actttccgaa aatacgatca agtgagaagc aaaaacaatg
    12661 tgactggtac acaagcagca caatatatat tacaaagtca acagataaat aatgtcggtg
    12721 ttcaacaaat tgccggagat ctcacagata actacaattc aggtaataag atgttgagtt
    12781 tgtctcaagc aactgcacag tctacttcag ttgctgcgat aggtgtagca gctcatgaat
    12841 gcggacatgc ggtacaagat gcttcgggtt atgtcccatt gaagattcgt gcagcacttg
    12901 ttccagcagc taactttggt tcgatgattt cgttcccctt gattatggta ggtgtattgc
    12961 ttagctggaa ctctacgtta atcaatatcg ggattttcgc tttttcttta gcattacttt
    13021 tccaattggt gacattacca gtcgaattta acgcgtctcg gcgagcaatc caaattttga
    13081 gtgaaggcgg actactgacg gaagaagaag taccgatggc aagacacgta ttatttgcag
    13141 cagcactgac ttatgttgca gcagcgcttt cgacattcct gcagctgttg cgcttgatct
    13201 tattgttcgg cggcaaccga agagattaaa ataaagaagt tgtgaaatca gcgctttgat
    13261 ctgagctaat accgagagtt agctgattga ataccattta tttggaata
//
LOCUS       DACDIE010000064        13206 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.064, whole genome shotgun sequence.
ACCESSION   DACDIE010000064 DACDIE010000000
VERSION     DACDIE010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13206
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.064"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..913)
                     /locus_tag="IV250_002224"
     CDS             complement(<1..913)
                     /locus_tag="IV250_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAP8769474.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1341..1904
                     /gene="efp"
                     /locus_tag="IV250_002225"
     CDS             1341..1904
                     /gene="efp"
                     /locus_tag="IV250_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAP8769475.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            2390..3649
                     /gene="lysA"
                     /locus_tag="IV250_002226"
     CDS             2390..3649
                     /gene="lysA"
                     /locus_tag="IV250_002226"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAP8769476.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            3636..4436
                     /gene="dapF"
                     /locus_tag="IV250_002227"
     CDS             3636..4436
                     /gene="dapF"
                     /locus_tag="IV250_002227"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAP8769477.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            4439..5320
                     /locus_tag="IV250_002228"
     CDS             4439..5320
                     /locus_tag="IV250_002228"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAP8769478.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            5317..6033
                     /locus_tag="IV250_002229"
     CDS             5317..6033
                     /locus_tag="IV250_002229"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAP8769479.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            6039..6734
                     /gene="dapD"
                     /locus_tag="IV250_002230"
     CDS             6039..6734
                     /gene="dapD"
                     /locus_tag="IV250_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAP8769480.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            6750..7751
                     /locus_tag="IV250_002231"
     CDS             6750..7751
                     /locus_tag="IV250_002231"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAP8769481.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            7748..9109
                     /locus_tag="IV250_002232"
     CDS             7748..9109
                     /locus_tag="IV250_002232"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAP8769482.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            9069..10220
                     /locus_tag="IV250_002233"
     CDS             9069..10220
                     /locus_tag="IV250_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAP8769483.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            10195..11364
                     /locus_tag="IV250_002234"
     CDS             10195..11364
                     /locus_tag="IV250_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP8769484.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            11539..12918
                     /locus_tag="IV250_002235"
     CDS             11539..12918
                     /locus_tag="IV250_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP8769485.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
BASE COUNT         4399 a         2300 c         2873 g         3634 t
ORIGIN      
        1 agtaaatgtt ttttgataaa tagcggtctg ctgcgtctgg aaaaatcgta acgacacgat
       61 gaccaggtgg taaacgacga atttctctta atgctgctgc aaaagccgca ccactcgaac
      121 ttccaactaa tagtccttgt tctcgtgcta actgcctagt atagttaaac ccttctacat
      181 cagagattgt ctcgatctga ttgattgaaa ggggagaaag aaacggcgga atgaattcta
      241 caccgatacc ctcaatttcg tgaggggctg gatcaccacc atttaacaca gagccctctg
      301 gttctacacc gattattcga atgtcgggat atttttcttt caaaaaggta gaagttccag
      361 caaaggttcc accactccca attcctgcta caaaactatg gatattttct ttgatttcat
      421 gaaagatttc tggccctaac gtatgatagt aagcagcagg attatcttta ttttcaaatt
      481 gaagaggcag atagctgttt gatatctcct ctgcaagttt tttacttttg ctgatggcac
      541 cgatgattcc ttgctcacta ggagaatgga tgatctttgc tcccaatgct ttcatcaatt
      601 gttgtttttc taagctaaac ttctctggaa caacaaaaac cgttcttaat ttgtatgtga
      661 gtgcggccag agcgatccca attcctgtat ttccggcagt tggttcgatg atcgtcgttt
      721 tttcattgat ttttccttgg gcaaatcctt ctttgatcaa ataactgccc aatctatctt
      781 tgatacttcc tccaggattt aaatattcta gttttgcata aatagcagaa cctttaggca
      841 tagaatagtg ctgattgtca aagcgataaa gcggtgtgga accaatagct tctgtaattg
      901 tcgagataat catgtgggcc tcctaaaaaa agataataaa aaaaggagct gactattaaa
      961 tcagctcctt gaaagacata gagaaaaatt gagtagataa aaagataagt tacctggtac
     1021 tcattctatg aagggcaatt aatagacatg acaaatagta tgctgctttt atgcaccata
     1081 caacagcaac gattcaattg tttttggttg ctgactgtca tgtgtatccc tccaagtaaa
     1141 atttttatta acgatagcat gaaaaaaaat aaaagtcaaa ggcttgaaag aaaaaaatat
     1201 ttcgatagca tcgtttaccc gtgtttattt ttctgacaaa taggcatgag accttaaaag
     1261 atagagaata ctctagtttt ctggccggtt gtctgataca ataaagttga ttatgataaa
     1321 gaaaagaagg agcaaaaata atgatctcag caagtgattt aagagccggt atgactttcg
     1381 tacaagacgg caaactgatt aaagttctgg atgcaagcca ccataaacct ggtaaaggaa
     1441 atacagttat gcgtatgaaa ctgaaagatg tccgttcagg tgcaacatac gacacaacgt
     1501 tcagaccaga agaaaaattt gaaaaagcac atatcgacac aaaacctgta caatatttgt
     1561 acacgatgga tgatacagct ttcttcatgg atttggaaac atatgagcaa tatgaaattc
     1621 ctgtagaaac agtagaagaa gaaatgaaat atatcctaga aaacatggaa gtaaaaatcc
     1681 aatttttcgg tagcgaagtc atcggcgttc aattaccaac aactgtcgta ttacgtgttg
     1741 tggaaacaca accttccatc aaaggtgcaa cagtcactgg ttcaggtaag ccagcgacaa
     1801 tggaaactgg cttagtagtc aatgttcctg acttcatcga aacagatgaa ttgttggaaa
     1861 tcaatacagc agaaggttct tatgtaaaac gtgcgtcaaa ataattcgct aacggattga
     1921 taagaatttg aaaacagagc ttgacctctt gaaaggcaga tcatcggatc tgcctttcaa
     1981 gaggtttttt gtggaatcag caaattgaaa aataaaaaag tatttgattt tatttatatt
     2041 ttgaaagatg aatggcaaaa gctagagaaa gaaaaaactg cacttattta tgaagaaatt
     2101 gaaatgattc ataaaattga aaatatccga actattccga actatattga agcgaaaata
     2161 ttttttcatc actaaatagt gttgaattta tgtttttatg tataaatagc ttctaatagt
     2221 tcgggtattt tgtttttata accattttta ttcttatttt taaaaaaata tttcgaaata
     2281 tactagacaa ttcagaaaat tttgtttaat attattccaa taaaattcaa atctttatca
     2341 aatgaaattc caataaaaac ggaaaaaaag tgaaaagagg aatgaaaaaa tgacagaatt
     2401 gataattggc ggagtcacag caagcacatt ggcagaaaag tataagacac ctctgtatgt
     2461 gtatgatgaa gaaaagatga gacaaacgat gctgtcattc aagcgaggct ttgtatcgaa
     2521 acgttttgat acaaaagtgc tttacgcctc taaagcattc caaacgattg agatgctgca
     2581 tttagttcgt gaatatggat tcgggctaga tgttgtcagt ggtggggaaa tctataccgc
     2641 tttgcaagct gattttccta gagatactat ctacttccat ggtaacaaca agacgccaga
     2701 ggaactgatc tatgcacttg aaaatgattt gttgcatatt gttgccgata atttgatgga
     2761 agttgaatta ttagcagaat taagccaacg ctatttaaaa aaaatctgta tcatgcttcg
     2821 gttgaatgtc ggagtagaag cacatactca tgaatatatc gtcactgcgc atattgattc
     2881 aaaattcggc atgagctatg aaagtaaaga gtgccaacaa gcccttcagt tgataaaaga
     2941 aagcagctat ctagagttag aaggatttca tgctcatatc gggtcacaaa tttttgatgt
     3001 tactgcttgg ttagctgaaa tcgacaaatt gacgaattac ttaaccgatt ttgaccaacc
     3061 tctttccttg aatcttggcg gagggtttgg gattcgttat acacaaaatg atcagccgat
     3121 cccagtcgaa gaggcactaa aaaaactgat tgaatatacg gaagaagctt tggaaaaacg
     3181 aaaactgacc attcgtcaat tactgatcga accgggtaga agtattgttg gagaagctgg
     3241 tacaacacta tacacgattg gtttcatcaa aaagacacca cataaaaaat attactttgt
     3301 agatggcggg atgacagata atatccgtcc agctctgtat caggcagaat acgattgtga
     3361 tttagcagca aaactgtcgg aagaaaaaac agagaaagtg acgattgcag gaaaaatgtg
     3421 tgaatcaggc gatgtggtaa ttaaagaagc ttatttgccg aaagcagaat cgggtgatct
     3481 tttagctgtt tattccacgg gagcttatgg atactcgatg agcagcaact acaatcgagc
     3541 gacacgacca gcagttgttt ttgtcaataa tggccaatca agactgattg ttcgcagaca
     3601 agcattttct gatctactaa ggggggaagt ttcctatgat ccttcataaa tataatggct
     3661 gcggaaatga ttttatctta ttagattacg cagaagacac agattatagt caattagccg
     3721 cttctttatg cacaaaagaa aaatatgata tagacggatt gatcgccgta aaaatcgatc
     3781 cgttggaaat gatctattac aacaaagatg gcagccgcgc gccgatgtgc ggaaatggca
     3841 ttcgctgttt tgctcgatac gtaaatgatc agaacatgat gaatgaacag gaattcaacg
     3901 tagagacatt agcaggcaca agaaaaatta cggtgttgga tccctcacct ttttattgca
     3961 gcgtagatat gggagtgcca gatgattctc cggaaaaaat caaagctaat caaacttatc
     4021 caataaaaaa ccaggaaata gagatcaatg gtgaaaaagt aacgatcact agtttatttt
     4081 taggaacgat tcataccgtt gtttttgttg aaaatgcgcg tgaggaagtc tggagaaaaa
     4141 gaggtgaact gatctgtcat catcctttgt tcaaagaaaa aaccaatgtc aactttgtag
     4201 aagtagtcaa tgaacaagaa ttgatcgtcc gtacctatga aagaggagta ggttggacac
     4261 ttgcttgcgg tacaggttgc tgtgcctctt atgtggttgc taaggaaact gggaaaattt
     4321 cagcagatca ggtagttgtt cacttggaac aaggagactt gatgatctct ggtgaggatg
     4381 cgatacaaat ggctggccca gcagttcatg aatggacaag aatattggag gaatgaagat
     4441 gataaaaggt tcgatcgtgg cgctgatcac gccgatgaat gaagagggat cagtagatta
     4501 tgctggtttg gaaaaattga tccaattcca tttagatgaa caaacagatg gattattagt
     4561 tttaggtaca acaggagaat cttctacgct cacacaatct gaggaggagc agatccttca
     4621 actgacagta aagaaagtgg ctggccgtgt gccggtgatt gcaggagctg gaacgaataa
     4681 tacgaaagaa acgattgaaa aagcgaagca ttttgcttct ttgggggcag acgcactttt
     4741 ggtcatcacc ccttactaca acaaaacaag tgatgcagga ctagctgctc attttacagc
     4801 aattgcagaa gcctcaccga ttccgcttat tttgtataat gttccttcta gaacaggcat
     4861 gtcgattcca atccatgtat tagtaaattt agcagaacat cctaatatca ttggtttgaa
     4921 agaagcctca ggagatatgg cttatgtgat ggatgccgct cggttgattg gagaagaatt
     4981 cttcctttac agtggaaatg atgatttgat cctaccagta atgagtgtag gaggaagcgg
     5041 tgtaataagt gtatgggcga atatccaacc taaaatagtc catgaattag taaaagatac
     5101 gcaggacgga agatggcagc aagcaaaaga aaagcaatta aatgcacttg aactgatcca
     5161 tgccttgttc agtgagacca atcctattcc tgtcaaagca gccatgtcat tattagactt
     5221 accttccgga cctctacgcc tgcctttagt ttctctttca aaagagaaaa agaaacaatt
     5281 agctcagctt ttgttaaaag aaaggacggt tgaaaaatga aaatcggact gattggcagt
     5341 ggacagatgg gaaatagagt cgaagaagtt gcacaagaaa gaggagatac ggtccttctt
     5401 ttacggacgg cacctaaaga agcaactacg cttgctttta cggaactgcc agaactgctt
     5461 attgattttt ctcatccaga taatttagag aaaatccttt cgtatagctt atcatatcaa
     5521 cttccgttag tcattggcac gacaggttat acacaatcac agttccaaga aataaaagaa
     5581 catgccaaac atgtacctgt gatgtatagt gctaattttt cgttcggcat catggtgatg
     5641 aatcaactaa tcaagcaagc agctgcaatg ttgcaaggct ggcagatcga actgattgaa
     5701 aaacatcata gtcgtaaaaa agatgcacca tctggtactg caaatatgct gcttcaaaca
     5761 atccaagaag tgcaaaagct gcagcccgta tacaactggc aagggaaaaa aagagaagaa
     5821 aaccaaattg gtgtacacag tattcgtgca ggttctttac ctggtgaaca tgatgtattg
     5881 tttgctgcta ctgatgaaat atttacgatc aaacatgaat ctttttctaa tcgaatcttt
     5941 gctgcaggcg cagtagaagc agcggactgg ttgaaagatc agtcaccagg ttattacaaa
     6001 ttagaagatt tattaatgga caaaggggtg taaaaaacgt ggaagctcaa gaaatcatcc
     6061 gctacattca aacggcatct aaaaagacgc ctgtgaaagt ctatctgaat gtaaccgagc
     6121 caatcgaatt taaagaaagt aaagtttttg gtgaaggaag cagcttcgtt gtttttggtg
     6181 actatgagac gattgctccg gttttagaag ctgaatcaga aaaaatcatt gaaatcgaaa
     6241 tcgaaactgc tgcaagatac agcgcagttc ctttgctaga tattaaaaaa attaatgctc
     6301 gtatcgaacc aggtgctatt atcagagatc aagtgtcaat cggcaacaat gccgtgatca
     6361 tgatgggagc gattatcaat attggtgcag taatcggtga aaacacgatg attgatatgg
     6421 gtgctgtctt aggtggacga gcgacagtgg gcaaaaactg ccatattggt gcaggagctg
     6481 tcttagcagg tgtgatcgaa ccagcatcgg ccaaaccggt tatcgtagaa gacggcgtac
     6541 tggtaggtgc gaatgcagtg attgttgaag gtgtccatat cggaaaagat gcagtcgtgg
     6601 cagcaggagc agtggtttta gaagatgtgg cagcagaaac tgttgtcggt ggaatacctg
     6661 cacgtgtatt aaaaataagt gatgatcaga caaaagaaaa tacagcattg attgctgcgt
     6721 tacgcgaatt atagaggaga tggagaaaaa tgcgcgaaat agatacctgt attgtggggg
     6781 caaccggatt agtcggccaa acgatgctca aagtgttaga agaatatgat tttccagtag
     6841 gacgattgaa attattagct tcaaaacgtt cagccggtaa agtttgtgta ttcaagaaaa
     6901 agaaacacac tgtacaggaa ttgacagaaa cttcatttga aggatatgat ttagcgctat
     6961 tttctgcagg tgcttctgta tccaaagagt ttgcgccaat cgcacgaagt cagggactat
     7021 ttgtcataga taattcctca gcatggcggg aagattcgga tattccatta atcgttccag
     7081 aggtcaatct ggaagactat acattgaatc ggctgattgc aaatccaaat tgttcaacca
     7141 ttcaagcggt acttccgctt aaagctttac aagaagcctt tggtatccag cgagtcaatt
     7201 attcgaccta tcaagcagtt tctggttccg ggcaaaaagg cattgaagat cttcatcgta
     7261 cgcaaaatga cgaggaacca gttttgtatc cttataatat tagccaaacg gtgattccgg
     7321 aaatcgatca gcctttggaa aatgggtata caaaagaaga acagaaaatg ataaatgaga
     7381 cccgtaagat tctgcatcag cctgctttgc cagtatcagc aacctgcgta cgtgtgccaa
     7441 tcgaaaatgg acatggagtc tctgtggcag ttcaactgca aaaagagttt acgacggaag
     7501 aaattagaga gtgtctcagc cagtttccag ggattcaatt agaagatgat ctagcagcac
     7561 ataaatatcc gacgagcatt cttgctagag gaacagatat ggtttatgtt gggaggatca
     7621 gaaaagacac ttctttagag aacggattgt tgttctatac gactgccgat aacatacgca
     7681 aaggagccgc tgcgaatgcc gtacagattg ctgcagcttt aatgcagaaa atagcggagg
     7741 tggcaagatg agtaatacgc tgaaagtagc gaaattcggc ggaacatcga tgtctcatga
     7801 agagcaattc aaaaaagtcc gtacaattgt tctttcagat cccaatcgcc aagtgatcgt
     7861 tgtttctgct ttaggcaaac gtacgtcaga tgatgacaaa gtaacagact tactttattt
     7921 gatttatgct taccttcagc atcatgttga ttggacaccc ctttggaaaa ggatttgtga
     7981 tcgtttcatt caagtaagag atgttcttgc cctgcagtta cctattgaag atgaactagc
     8041 acgcatacag aaacaactgg aagacgaaaa gataactgaa gattatttag taagtagagg
     8101 tgaatatctg actgctcaac tgatggctga gtatcttggt taccagttta ttgacgcaaa
     8161 agacctgatt ttctttgact atgatgggaa aatcgatcag gaaaaaacag cacaggaaat
     8221 cacaaaagca tttaaaagat atccgaaaat ggttgttcca gggttttacg gggcgaaccc
     8281 tgcaggaaaa gaaaaattat tgggccgtgg gggaagcgac attaccggcg cgatactggc
     8341 gaagatgttg caagcggaaa gttatgagaa ttggacggat gtttcaggaa tcatgatggc
     8401 tgatccgcga atcatcgatc atcctaaaat catcgaggaa atctctttta gagaactgag
     8461 agagatggct tatatggggg ctaacgttct tcatgaagca gctgttttcc cagttcaaga
     8521 ggctgatatc cccatccaaa taaaaaatac caatgatcct ttcgctcaag ggacaaaaat
     8581 atcgaatcac gaaatagaaa aggaaaatgg actgacagga atagctggac gcaaggattt
     8641 tctttccatc actttattca aacgtcatat gtccgatgaa atcgggttta tctggaaagc
     8701 aatgagcatt tttgctaaac atgagatatc aatcgaacat attccttcag ggatcgacaa
     8761 tatcggagtg atcgtctcag cagaagcgat tgccgataag ttatttctca tcacgaaaga
     8821 attaaaagaa acacttggcg tggaagaaat cgtcgtgatc gaagacttag ctttgatctc
     8881 tgtcgttggg ggtccgcata aagaattgat tggattatct gggaaagtgt tatcgatatt
     8941 gaacaaatta gaaattcgca catcgatctt atcacaagga gcacaggagt tgaatctgat
     9001 catcggtgta ccgaataatc aatatgaaac agtagtgaag ggtatctatg aagggatggt
     9061 gaatacggat gctgaaagaa tatcgaatgg cactccatca aattcctgaa atcgggtttc
     9121 atgaattcaa gacaaaaaaa tatttatatg aacaagtaaa aaacagcgag gccgttatcc
     9181 atgaaatcgg cgaaacagga ctactccttt attttgataa tcagcaagaa gaaacgattg
     9241 cttttcgaac agatatcgat gcactgccag tcaaagaaga aacgaatctc tcttttgctt
     9301 ctaagcatga aggttatatg catgcatgtg gtcatgacgg acatatggcg atgcttttag
     9361 ggttgactga ctttataact aaacacattg aggatcttat ttataatgta gtactgatct
     9421 atcagccatc agaagaaatt tccggtggcg cacaaagcgt tattcagtca ggattgttgg
     9481 ataaatatga agtacaagca atatttggct ttcatctatg gcctggacta gctaagggag
     9541 aagttttctc tcgaccagga cctttgatgg cacaaagcag tgaaacagac attatcatta
     9601 aaggccagtc tgcacatatt gcttctagtg aaaaaggaat cgatagtctg gaggcagctg
     9661 tacgattttt gaagaatgtt tatgattttg accggcaatt accggcagag tggaatcatc
     9721 taatgaaatt tggccaaatc caaggtggaa cgatccgaaa tgttttagct ggagaagtta
     9781 ttgtatccgg aagtatccgt tcttacagtt cagatatgca aaattatttg aagaggggat
     9841 tagaccattt agcagaacag tttcactcta cagctactat ttcttttcgc tataatgacg
     9901 gctatccagc tgtaatcaac catagaggct tatatgcagc actgttagat tcaaacaaac
     9961 tacatgaatt agatgctcca gttcttcaag ccgaagattt tggcatatat acgcaacaat
    10021 atccatgtgt atttttcttt ttaggtgtcg gtgatacccc tgctttacat gatccgacgt
    10081 tcgattttga tatggatgtt ttagaaaatg gtgtagaatg gtacaaaacg attcttttta
    10141 cagaaaatct tcttgtaaaa atcgatagca gttcaaaaga ggagcgagta aataatgatg
    10201 aatttatcca atcgatttag tcctaggctt tctagaatag aagtatcaaa aatacgacaa
    10261 ttcgatcagc aaatatcgtc gataccagat gtgatcaaac tcaccctggg agaaccggat
    10321 tttccaactc ctgaacatgt caagcaagca ggaatagcag cgattgaaga agacttttct
    10381 cactataccg gaatgagagg attagaggaa ctgcgggaag cggcttgtat atttcagcaa
    10441 cagcgttatg gattaactta tgatccgcaa acggaagttt tgacgacagt tggtgcaacg
    10501 gaagcgattg cctctgcgtt gttatctgtt ttggaagaag gagacaaggt tcttattcca
    10561 gcgcctgctt attcaggata tcagccatta gtggaactag ctggtgctga attgatcccg
    10621 atagatactt ctgacacagg gtttgtctgt cagccggaac aatttgaacg agcatttgaa
    10681 caatatggat cagctgtcaa agcagtcatt ttaaactatc ctaacaatcc tactgggacg
    10741 acattgtctg caaaacaatt gaaagcaatc gctgaggttt taaaaaaata tccggtattt
    10801 gttataagtg atgaagtata tgctgaattg acttatagtg gaacgcatat gtccatagct
    10861 acttatcttc cagaacaaac aatcgtgata agcgggttat ccaagtcaca tgcgatgact
    10921 ggttggcgag tgggctttat ttttgctcaa aaagccctta ttgacgaact aattaaagtc
    10981 catcaatatc tggtgactgc cgctacaacg atgtcgcaaa aagctgcggc tgaagcgcta
    11041 ataaatgggg tgaaagatag cgagaatatg aaagagcaat atcaaacacg aagagactat
    11101 ctggtccagc aattggcgcc tttaggattt gaagtgaccc agccaaacgg tgcattttac
    11161 ttgttttgca aactcccagc tgccattcaa actgactctt ggcagttttg tgtagaatta
    11221 gcggaacagg caaaagttgc gtgtatccca ggaattgcat ttggcccaga aggagaaggt
    11281 tatatccgga tttcttatgc tagcagcatg gaaaaattac atgaggcctg caaacggatc
    11341 aaagactttt taacagaaca gtgattcata gattttatca aaaaaacaat cgttaatttt
    11401 gcggtatatt ctgcaaggag acgattgttt tttaaattta gttcaaatga acgcgtttct
    11461 tttgattcat aactaatgta atgttaagtt taaattgttt agaatgattt taaaagaata
    11521 ttcaaggagg tttcatcaat ggaaaaaatc aaagttgtca ttgtaggtgc atctcatgga
    11581 gggcatcaat cgatcttgga actattgaca cgatacggtg aaaatgtaga tatcacgcta
    11641 tttgaagcag gagactatat ttccttcatg tcatgtggta tggagctgta tttagaagac
    11701 aaggtaacag atgtaaatga tgtacgtaat ttcagaccag aaaactttcc gcaatccaat
    11761 gttcatattt taaataatca cgaagtaaaa acgatcgatg ccgataaaaa aacagtaacg
    11821 gttactagcg catcagataa cgaaacaaaa gaatatcctt atgacaaatt gattttgagt
    11881 tctggtgtca aaccaaacaa tctgccagta ccaggagcag atctagaaaa tgtctacttg
    11941 atgcgtggat acaattgggc aaccaaaatc aaagaaaaat taaatgatcc ggctattaaa
    12001 aaaatcgctg tcgtaggtgc gggttacatt gggatcgaag cagcagaagc aagccgaaaa
    12061 gctgggaaag aagtcgtatt gcttgatgtg atcgatagac ctttaggcac ttatttagat
    12121 gctgagatga cagatatttt agaacaacac ctaaatgaaa acggtattga agttttgaca
    12181 aatgcaaaaa tcgaagcttt tactggtaac ggaaaagttg aggcaatcaa aacaagtgaa
    12241 aaagaaatcc cagcagatct agtcattcaa gctgccggtg tgaaggcaaa tacagaatgg
    12301 ttaaaaggga tcgtcgactt agatgaacgt ggctggatcc aaacaaatga atacttgcaa
    12361 acgaatcttc cagatgtgta tgcagtagga gatgctacac ttgcttattc tatcccagca
    12421 cgcaaaaaaa tgccgatcgc tttagcgact gttgcacgta gagaagcacg ttatgtagta
    12481 aaacatcttt ttgaagaagt accaaacaaa ccgttcggcg gagtcgtagg ttcctctgca
    12541 ttaagtgtct ttgactatca ttttgcagca agcggattga atagctttac agcagcaaaa
    12601 gcaggagtag atgtacgagt gtcttattat gaagatacgt tacgaccaaa atatgtacca
    12661 gaagaacaag gaaatccaaa agtgtccatc caattattct cggatccaat gacccatcaa
    12721 ttgctaggag gagcagtatt atctacctat gatgttacag gtcaaggaaa tgtattggca
    12781 ctagcgatcc aaaacaaact gactttagaa gaactagcag aagctgactt cttcttccag
    12841 ccaggctttg accgacaatg gagcatgttg aatctagccg ctcaacaagc gttaggagaa
    12901 gaaccatttg tagaataaga aaacgaaata tttgaaaaga ggttgtgaaa aagctgcctt
    12961 gcatcaagta ataagaacag ccaagtaaaa acagcttctt atgtttttaa cttggctgga
    13021 cttattaccg cagatgcaag cttttgaaca ccgtttattt tcaaaaaagg gactgtgaca
    13081 cgatttgtgt accgaccccc aaaagttaga ccaaaaaatc taacttttgg aggtcggtat
    13141 tttttatggt taaatatact tttgaactta aattaaagat cgttcatgat tatttggatg
    13201 gcaaag
//
LOCUS       DACDIE010000065        12772 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.065, whole genome shotgun sequence.
ACCESSION   DACDIE010000065 DACDIE010000000
VERSION     DACDIE010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12772
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.065"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..1269)
                     /locus_tag="IV250_002236"
     CDS             complement(<1..1269)
                     /locus_tag="IV250_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HAP8769486.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQI"
     gene            complement(1366..1866)
                     /locus_tag="IV250_002237"
     CDS             complement(1366..1866)
                     /locus_tag="IV250_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP8769487.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            complement(1879..2100)
                     /locus_tag="IV250_002238"
     CDS             complement(1879..2100)
                     /locus_tag="IV250_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769488.1"
                     /translation="MNFGQNLYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFLV
                     IALIAVGLVFNASGVKDVLLNLFNRIVGA"
     gene            complement(2105..2224)
                     /locus_tag="IV250_002239"
     CDS             complement(2105..2224)
                     /locus_tag="IV250_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAP8769489.1"
                     /translation="MEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKGK"
     gene            complement(2239..3423)
                     /locus_tag="IV250_002240"
     CDS             complement(2239..3423)
                     /locus_tag="IV250_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP8769490.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(3679..3933)
                     /locus_tag="IV250_002241"
     CDS             complement(3679..3933)
                     /locus_tag="IV250_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769491.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            complement(3958..5100)
                     /locus_tag="IV250_002242"
     CDS             complement(3958..5100)
                     /locus_tag="IV250_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HAP8769492.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            complement(5160..6512)
                     /locus_tag="IV250_002243"
     CDS             complement(5160..6512)
                     /locus_tag="IV250_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP8769493.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFWVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            complement(6583..6927)
                     /locus_tag="IV250_002244"
     CDS             complement(6583..6927)
                     /locus_tag="IV250_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAP8769494.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            complement(6937..7311)
                     /locus_tag="IV250_002245"
     CDS             complement(6937..7311)
                     /locus_tag="IV250_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP8769495.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            complement(7324..7638)
                     /locus_tag="IV250_002246"
     CDS             complement(7324..7638)
                     /locus_tag="IV250_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP8769496.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(7752..10979)
                     /gene="fss3"
                     /locus_tag="IV250_002247"
     CDS             complement(7752..10979)
                     /gene="fss3"
                     /locus_tag="IV250_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAP8769497.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNETQGKAEFKGAEYTL
                     FTAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITDAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            complement(11058..11720)
                     /locus_tag="IV250_002248"
     CDS             complement(11058..11720)
                     /locus_tag="IV250_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769498.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            12337..12567
                     /locus_tag="IV250_002249"
     CDS             12337..12567
                     /locus_tag="IV250_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769499.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENNQ
                     PVIAKKETVLSEGKIKTRYILEVETKFKNRSE"
BASE COUNT         3610 a         2831 c         2096 g         4235 t
ORIGIN      
        1 aatttgtttt tgagagtata tactcacctt atcggaaatc attctcaacc gttctgtttc
       61 tttttcaacc agaccattct cttgtgtaaa ttcataaata gaatgtctat cctgtgttct
      121 tattatttga tattttgtcc ccttatagaa aatattcagt ttcacaattg tatccttcaa
      181 aaatattcca gagccattat gttcaatttg tgcaaagttc tgataatcac tttgtatttc
      241 agaaaattga tcaagttctg cttcctttcc aaggacatag accaagaatc ttgtaataca
      301 agatttgcct gttcctcttc ctccaataat tgaagttaaa ttatcgctga attcgactac
      361 aacttctttt gtgctaagag tggtcccaga aaagcttaat ttttccaata aaacttcatt
      421 ttcattaatg gagaaattag aaaaatcaga atgaactcga gtttcgccta gcattaaagc
      481 atcgcgaagt gatcccaagt caggttcgtc cttcattttt atataggaat atcttgtgcc
      541 tattccccaa agtccatgtt ttgaatttcc tttcgagtgt gggttatcag aaaagctaag
      601 aaatgtcata ttattttttt ctgcctcaac aactgattta taccaagatt ccaaagtatc
      661 ttttccaact ctaccatcat atctggcatt cacactatca tagatagtgt cacgcttaga
      721 tgagggaatc atttgtgagc gattttcaaa aaattctttt tgaaccgtct gtactgcata
      781 tatattttct gattcaaata tctttcctag atttccaaat tttgctgaag aaattccatt
      841 aaactcatct atatgtgcag gaataacgag caagccctcg tgctcagcaa aatcaataac
      901 ttctaaaact gatttcattg agcaagctgc agcgctggcc caatcctctc tttttatacc
      961 catcttggtt aagaaatcag agatatcatt tgtacatgca cttttatcaa ataggactag
     1021 taaatgagtt ccattttctc cacacgttaa ttccactcca ggaaaaaaat ctaagccatt
     1081 ttcgatagcc actttcttat attcatcaat attttcccct gtattgtgat cagttaatgc
     1141 aacacaatcg agtcctttgt ttaaacattc ttctacccat tccttagcag taaccttttt
     1201 atcctgaaag cactgactcg cagttgaatg aatatgaaga tcacatttgt accacttatt
     1261 gccaaccatt agtaacaccc ctatcaacaa atattttcta tttcagtata tcaaatagaa
     1321 aagtaaaagt acgatagaaa tttctggtca attctaacaa atgatttaca ttagttcaaa
     1381 aacccctcga ctcgtaacca aataattccc gccaatctcc aagtctcgac cacacgcatc
     1441 aaaatcaata tacgtcttca agtgttctgg tacttcgcct aaatcaccag cttctacaat
     1501 caagtaatac gctacatcac tcatgtcatt acaatcggga taatagatga tatcatccac
     1561 atgctctacc atttcctcga aagaattgaa gaaggcatgt tgaatctctg aagcgacctc
     1621 accaattggc gttcctttaa gctcttccgc taaaccacac aaccgattaa tttcctcaat
     1681 tggtgtatac tcatcaatct caaaaggcag ttcataatca tgaattgcgt actcttcata
     1741 ttcgtcattt aatccaatcc gttctttcac ttcatcaaaa tcgacgggtg gggtaaacca
     1801 tgcccccacc aactcacctt cattgtattt tccaaggttc gcaatataaa cacgtatctg
     1861 ttccattgtg attccccctc aggcaccgac aattcggtta aacaaattga gtaagacatc
     1921 cttcacacca gaagcattaa agaccagacc taccgcaatc aaagcaatca ccaagaagcc
     1981 aatcagtttg gaaaactcgc gtttaaatcc taaaaacaaa ccaatgacca caatcgctag
     2041 taagactaaa gattgagcat tggttaaaaa ccattgatac aaattttgac cgaaattcat
     2101 ttcatcactt tcctttcttt tccattgctc ggtccgccgc tgcacttgca tgcatcaagc
     2161 acatcacagt aacaccaagc atactgccac caaacaccaa taaaatatct ttcacaattt
     2221 ccatcacgat tcctcttttc atataatcaa attttcaatt gccatacttt gttgctccaa
     2281 aatctttttg tgccggtctg atagtcgagt attatttacc atatcctgaa tgtacgttgt
     2341 ctgattcaac cgatccaact ccttggccat cttccacgta ggggcgactt gtcgtccaag
     2401 ccagttcaac gttcgttcaa tggtataagg cttcggttca gaagttaatt gaatttcctt
     2461 ctgattccgt ccaatgaagc gcccccattg ttcattcaag ggccagttag ttcgcctttt
     2521 gccttccacc ttatccgcaa atcgcaagta ccggttaata atggaaaatg tggtactttc
     2581 aatactgcga ccttttaaca aatcttgaat tgcatgataa gctcgatcat ttttcagtcg
     2641 gatttcaaaa cgatttttcg tttcggtctg gtctaaagga atccctaact tgatatattg
     2701 ctcatagtct ttttcgtaca aacagaaata gacctcactc ttaagactgc caatatacaa
     2761 agtattgccc ataccatctt tatcatcagg tttcaacagt tccccggaac gatagctttt
     2821 gaaggtacga aacaaggaga tacattcttc cttctgacat tttttcgtta aatcaggaat
     2881 atctaacagg ccaactcgat cattgattgc caggtctaag cgtttcatca cgccaccggc
     2941 ttttagacaa tcttcgaaaa agtcatacca gctacgcttt tgggcgagta aaaaagtttc
     3001 aaactgccga caaccacgac cctttaattc taaaaggacg cctttatctt cttcatgtga
     3061 caacatcacc acaatatctc ccataacata ttgttcttga taggagtaaa aggcgtaatc
     3121 ttcgtggagc atataatcaa atttcaaatg gagaatctct tgaatgattt tcctcgcatc
     3181 cgttgtggga aagcgaatcc ttacatagtc aaacagtaac gttaaaggta actcgggatt
     3241 gaaactttcc agttggtcaa aaattttcct catggttttt gtcgatgcgg ttacgccacc
     3301 attttctatc tgatttaagt acgggcgact aagtccacaa gtcgtagcta atttattttg
     3361 tgaaacgcca tattcattac gccggtcttt taatagccga taatctaaat tcctttgtgc
     3421 caagttctca cctccaaatt taaggtgtta actttttcaa atcagttaac atctacacaa
     3481 attgcttatt atccttgata ggacagccat tccttatcgt tgcttgctta ttttttgtgt
     3541 ttttgtaccc ccctgttaga tacggggggt gagacatcat ggccggctaa cgccgtccat
     3601 aaacaggcac agcaccacgg aggatttctg cttcgcaaat cccccgcggt cagtgcctat
     3661 cagtggttta tttggccgtt actccgcggt aataccctca atttccaact ctgcttttat
     3721 ctcaaacaag tattggcgga tatcaggttc tttagcaaag tccgcaattc gttccaattc
     3781 actgatgact tgattcagct ccattcgagc atccgtttcc tcatcaatta catacggatc
     3841 atcagggact ttcaccccgt ttttgcgaat atcaaaattg agccggtaca gttcttcttc
     3901 caattccgga tctgactgaa tcttgccaat cacccagtag attccccaat caactcatca
     3961 taaatcatct gtttttacct cctttaatct tctagcaata tcaaaaatga ccggaactgt
     4021 tacagaattt ccagcctgct tatacaactg actatctgaa ttgacttgag aagctcgatc
     4081 aaaagcccag tccggaaagc cctgaagccg ccagcactcc ctaggtgtta actttcgaat
     4141 ttgatactga tccgtgacga ctgcttgttg gtctcctgtt aataaggtat gcacactttg
     4201 ttttcccaga cggcctcgac gacttgtgga attaggaaaa gccaggttca cactgttccc
     4261 tggcttcacc tcttgatatt ctgtttcgtt tttccgaagc atgatcttcg ttccgccttg
     4321 tttcgttgac aaagtaggcg ctgttccagt tatctcataa acccgattgc cttgttcaaa
     4381 atttcccagt aacatacccg ctacagaaat tccatcatct accggcaatg cgattttgat
     4441 tccttcatca ccggtcaagg taggcgctac gtcatctgac ccatagactt gtccccccat
     4501 tcctcgattg gaagggttga tatttaataa gtttctgatg ttttgatcag atgttggaga
     4561 cgatccaatg ataggaaata cttgttcggt acatgcgtct cgagaatgtc cgataaggaa
     4621 gatccgctca cggttttggg gaacgtaggc ctttgagtta agcacttgcc attccacatc
     4681 ataccccaat ccatccagcg ttcggaggat cgtctcgaac gtagcccctc cttcgtgatt
     4741 gagtaatccc cggacgttct caaggaataa aaacttaggt tggagaatag cggcgaaccg
     4801 agcgatttca aagaataaag tacctcgagt atcggaaaat ccttgccgct ttcccgcaat
     4861 tgaaaaagct tggcacggaa atccaccgca aaggatatcc actggtccga gagattgaat
     4921 aaactcgtct gatacacttg tgatgtcatg catttccacc tccttacttg tatcatggat
     4981 tgccttataa ctctggcgag caaaatcatc aatttcacaa aagccgatac actggtgacc
     5041 cgcttgttcc atacctaagc aaaagccacc aatcccagcg aataaatcta aaaatgtcat
     5101 aaatcacctc gtaaaaaaag cccaggattc ttgacactcc taggctacat actattattt
     5161 tatccttccg tattaacgtt taatccacca atgtcgctaa tttcccttaa gaaatcatat
     5221 ccttttggta caagtggcgt ataaaactca ctaatcacac tccctcccgt atctacatag
     5281 cctcgacctt tgatacgctt cataaagaat tttttatcaa cctcgccaaa catcatggaa
     5341 tatcccagtt cactcatacg acccaaagcc acccagaaat taaattgatc gcgaatcccg
     5401 tctcctaaat atttcgcatc cggtctttga caagctagaa tcagaaagaa accagattga
     5461 cgacctaaca tcacgatttg tttgagctta ttcaaaatca ccgcactttc cttcgtcgtt
     5521 aacatttcca tataaaccac gtattcatca aagattaaaa agtttggtgg aagtccaaga
     5581 aacgcataat tctctcctgt tttgtagttg ggcatttctt tcattgcctt gctacgagtc
     5641 atcatgcgct catagaaatc ttccacacaa gcagaaattt cctccttttg ggaatagaca
     5701 tgaggcatta ccgtacctaa atcggctaag tccgcatttt tgggatcaag aacaaaaagc
     5761 tcggcatcag attttaataa agcctcgatg atagtgagta aaaagtaagt cttcccacca
     5821 cctgtgccgc cagcgattaa catatgaggt aaagaatcat aagcccatac ttgatttttc
     5881 atcaatcgca aagccccact ttctgccact acttcgtcta tcccaattcg attggcaatt
     5941 atatcataca gtaaagtgta ctccacatag gagtctttga gttccttttc tactagttca
     6001 caatatagac cactttctaa cttcttttcc agctttaaaa gctgatcttg atattttccc
     6061 agtgaaattt gaacgcgaat agaaagtaaa ccatccttca tacggtaata gattttgggg
     6121 aagtaactga ttgtctcttt ggttcgacta ctattgaggt ctttgaaaaa tccttcactc
     6181 tgagtttgtt ttacttcgta ccaatgattt tctagaatca tccgggctag cttttgtcga
     6241 tgccacaaac tgcgccaatg atcctgaaaa aagtgtatat aaagcaacat cgtgcttcct
     6301 gaaagaacga gacttcctag caaatagatt aaatctttta tagaacgaac gatttctata
     6361 gtagaaagtg atagcttaga aatatcaaat tgtctaagct cttgaaaata aaagacgctc
     6421 atccaaccga tgaacagagc gagtagccag ccaagaacaa agtgataaac caagtgctga
     6481 tctcctgccc gtatacgatg ccctttgtac atttttatgg tttccacctc caatcgaaaa
     6541 aagcttcata ggatttcatt gatccaattt caagcagtta tgctagtcat ttactgctat
     6601 agggagttgt ttcgtttcca ctagttgctt taaaaaaact tcaatggcat ccggcatttc
     6661 cacaccaatt tcttccaata cggtttcggc ttctcgacat agactttcat ttacttcgat
     6721 aaccataaag aaggactctt cttcccctaa ccaattcagt acatcaaaat cttcttcggc
     6781 atcgttacct acttccagta acccttgatg cataatcaga aacatttttt catcataggt
     6841 taatgcttta gtgagttgtt cctccgttag ctcacctttt ccatattgat cgagacaatc
     6901 taaaatatgt tcaacattaa tttccatacg cttcacctat ttctttcctt cattttggtt
     6961 tgtagaattt ccctgatctg gttgtcgttt caagactaaa tcatcggcct taatgaacca
     7021 attgacagta gttccttctc ggaaaacata attggcgacc gtatccatga ttggattgac
     7081 caactctacc tctgcgtcat aggcaaactc gcgtaagggt acatttgctg ggatactaac
     7141 ttggatcatt cgaccttgcg cagaggattt caaattataa gtccgttctt ttacctcgtt
     7201 actaaccgtt ccatcctcat tttgcttccg tacttctcgg cgtaatgcgg aaaatcgtaa
     7261 tttcccaaac gttagtcctg aatctacaac aataccttct gttaatctca tcttcttttc
     7321 ctcctatgct ttcactaaat catcgacata caacacgtag tcggtaaacc cacgattctc
     7381 aatctcatag cctttggcaa ccaaatgggg attcacggct tttagtggtt gatcttgatc
     7441 aaaatccttt tcgccggctt ccgcagctac caccacttca atatcatcgg ctcgttgctt
     7501 actggaatac aaatgataac tacgaccaat tactgtagta ttccgcccat atccttcagt
     7561 taaaacttcg ccttcgccgg cataccttat ctttccaaag gtttcggcca tatctggcac
     7621 aacaaatttc aattccatct tcttttcctc ttttcatttt tagttttgga tacaaaaata
     7681 gcgcgaccac tttttttagg aggcggtcgc gcacgttggt ttttcctatt tagttgtttc
     7741 atcacatcac cttagtttcg gcgattccag aaataatacc cagcagtcgc aaagatcaag
     7801 gtaaacccaa taaacagcag aacattagaa tttttctccc ctgtttgtgg gaatgtcttt
     7861 actgtaggac tagattcttg agagtcactt ggaacagttg gtatttccgg ttgttctggt
     7921 gcacttggtg ttgtcggaac ttttttaggt gttaaagttt gagttttttc ttttagatcc
     7981 tcattgtgtt tcccattgat gtttccgtct ttgccatagt taatttcttt gaaggtaaaa
     8041 cttgtacctt ctggatattt acttgtgtcc actttttccg caagtacggt tttagtgaat
     8101 tctttgtcat tcacttcata atcaattttg ccagatttcc aaatttcttt ggtcgtatta
     8161 tccggaagta atgcatacag aattgtttca aaagcttctt ttgagccatc cagtacatca
     8221 tgggtaatcg acacatcatc gaacatatcc accacatccc catgagtaaa ggtttgcgaa
     8281 ccatcttcta ggtgggcttt tgtttggatg gaaacatggc gttccaccgt acaattgaca
     8341 gtttgggctt gattgttcaa gctagcatct ttcgctactg gatcatcacc tgcttcatag
     8401 ggtttcttgt tttcatacac atagttgaat aagacgatgg ttttatccaa aacttgttct
     8461 gcggttaatt tatgcaaaat tttccaagtt cctgttttct ctttattcgc caacgttgcg
     8521 gttgtttcgg caattaccac tggtttggct ttttcatctt tttcttgggc agcttttgtc
     8581 gcttccacat caatggcttg cgctaccaca taccagtctt ctttgatttc atgcaagtta
     8641 tagctcaatt tatcaactag ctcggtagaa tcaaggtctg tcaattcttt gcctccgtct
     8701 ttccaagtcc caagagaagt taagctatct tcttcttcag gtaaatcata gagcatcaaa
     8761 cgattcaagc taacagaaaa tgctttattc gtcagtttct cgtaaggaac ggtcaccgtt
     8821 ttaattggtt gtttttgtcc tttttcggta atcgtaaaaa tgtattcact cttagtgtaa
     8881 tccgctttat tttctttaaa agtagaacgg atttctaatg gcgtaatttt ttgaaatcct
     8941 tctggagcct ctacttcttc aagtaaataa tccccataag gaagattttc aaacttgcca
     9001 tagccatcaa aacctagttg ctcgttataa gctgtaattg cttcatcttc ggcatctgta
     9061 atctcactgg ttccttccaa tggcgatact ttaaaggtca gatcattgaa gccagtttcg
     9121 gcagtcccag cagctgatcc agcaaattta aagaaatcaa agccaaagcg aatgacttgt
     9181 tcttttgccg taacatctcg ggtaatcacg gcattttttt cgttatcatc cacctttttg
     9241 atggaaacag ggtactttgt ttcatccaag gtatatcctt caggtgcttt ggtttcttgc
     9301 cagtaatact cattaatcgc taagtgttta acagcaactt gattcttttc atctaaagtc
     9361 aaagtcaccg tttcatcaga agctttcgtt cccttcacta actctggttt aaaagcctca
     9421 ctccatttga ccgcttgacc atcttttgca gtaaagagag tatattcagc tcctttaaac
     9481 tcagctttac cttgagtttc attaccggta tctttgtctt cttttgtcaa agtggtttca
     9541 ccagtgattt cttggttttg cccttttacg ttactggtaa caagagccac ggtttgattg
     9601 acatagttca attcgacttt tactggttta aaggtattca caaagccatt actagctttg
     9661 gtttcggtca cataataggt tcccaattcc aacgcattgg caatctcttt tggtgtttcc
     9721 gcatgacctt tttcatccgt ggtcatttct tggacaattt caccggttgg actgtctttg
     9781 cgaatggcaa acgtatttcc ggctaatgag tatttgtcgt tccaaagatc agttcctgtt
     9841 tccaccccca ttttattcaa aatgatttga cctttttcgc gagtattttt tgaagtcaca
     9901 gaaatagtct caccagattt aatggttgcg gtgattggtg tagtgtcaat cgtataagga
     9961 gctggcactg atttttcagt aatggttact ttcgcgccat gaggaatctc atccaatgtc
    10021 gcaatgcctt ctttatcggt ggtcacgtct tttgcaggta atgtttttcc aaagtctaaa
    10081 tggaaaacgg ttcccggtac aacatttcct gtttctttat cgactttttt aatcttcaaa
    10141 gaaccctcag tttccacatc aattttgatt gtgtaggtat tgggcttatc aattgccccc
    10201 gccatcaaag tttgttgacc agccatttta taagctaccg gtgttcctgt attagctgac
    10261 ttctttaggg ttaattcacc actctctttt gaattggcat ttggagtaat cactagttga
    10321 ttgccgttca cacgataatc aatatttgcg atattctcga caacttcatc aaactccgat
    10381 aaattcaagc cgttcgtatc tgtcacagta gtcgattgac ctaacacggt tttcactgtg
    10441 gagttatgga agcttggttt cttttgataa tccgcaatgg cttgattgat ttttgactca
    10501 atggctgcga tatttactgc actaccatca gaacgtttaa ttgagtgaag cgtgtagcca
    10561 ttaacttctt gccaaatcat cttctgggcc acaatatgtg tatccacatc cgtaccagct
    10621 tccttccata aaacggaaac taatttcgct ttctccgaca tatcaggcaa tggatttttc
    10681 tcataaccag gagtgaactc attgtaaatc ggcacccctg gctcaataca gaaaatatct
    10741 tgcttcacac cattgtaaac tgcatagtgc ggaacggtaa cttctgacat gggacgacca
    10801 tcgctaaatg taccttcgac agcatactgg tgagccaaat ccaattccac agttaccgtt
    10861 tgtggatgag taatttcatc agcaaaggca atcgttgggc caagcgatcc accaattgtt
    10921 tggcataata gactcagtgt tgcgagcaaa cgccaattct taaatcttgg ctttttcata
    10981 aaataaatct cctttcgttt ttgggtaata aaaaagacgt ccaaatttta attttgaacg
    11041 tctgatttag agtgatacta tttagttttc tttgtcttgg ataggttgtt tctctgctta
    11101 taaattttga ttcttcgata atcaaatcca atctctgcca tcaattcctc ccaatttttg
    11161 ttcgttcttt tcataattgt atcaactgat ggtagttttg gggattgaac tagttttcga
    11221 aactttgtca ttgataaatc atcctgtagt tttaatcgga ttatttcatt ttttagttct
    11281 aacagcagtt cgaaatcagt aaatcgagat ccatcatttt tttctttaaa aagtggacgt
    11341 atatctccta acatcttttt aatggtacta agtgaaggaa tattcttccc tactgatttt
    11401 tgttcgtaca ttcgttgaga ggttatcttc tcgcacttaa taaaggcttc tactatttcc
    11461 aataattctt gttctgacat tctagcccat tttccatacc cagtattttc tcgtcctata
    11521 ctaatcaata aattctccca tgatccatat tttttgttaa tgaaccacat acttggtgca
    11581 gaatttggat tttctgatag ctttttctgg tattgacggc tagatgtaat tccgtgtttt
    11641 tcgatgaatt cattaattgt taaaatcagt tttaactcac tttgattttt ccaaatgata
    11701 cgtccatcat tttgcttcac gataaaaact cctaatagtt atttagatag aagcctatca
    11761 catcaacttt ttagtagaaa agtacatgtt ttgtcttttt tgctaatagc tgaccctttg
    11821 agaaacttgg gttcaaaagt tgctaaattc ggtaaatgtt ttggtatttt tcgtattata
    11881 tttgtcgtca agctgactac taaattttgc cctattttga cgacactgat agtcaatgtc
    11941 ggacaatcta ggacaatttt taagggaata tgattgaaaa atggtccaaa acagccaaaa
    12001 atggcttaaa actgtgatag agaaaaaaga aaaagccctt gatatacaag gacttttcac
    12061 aaatttcaga tttccaagga agttttccgt gtttatcttc tgatttcttt gatacgagca
    12121 gcttttccgt gtaatgcacg taaacaaaac cactatattc tattatgtat ctaataccct
    12181 caaaacagct ataaatcaac aataccgaca tataataaac actatgttca atctgagata
    12241 tatcaaaggt aaacgtattt ttgccccctt tttgcccctc aaatatcgct ttacaacgag
    12301 aacgtaagtt cgtataatcg ttttgaggtg atttttatga tggaagactt tattagaaaa
    12361 aatatcagtg atgaatatgc agacttttat gagcaaagca gcaaaaaaga taaattccag
    12421 atggatgttt caatcttagc tatattagct ttttccgaaa ataaccaacc tgtaattgcg
    12481 aaaaaagaaa cagtattatc tgaaggcaaa ataaaaactc gatatatatt agaggtagaa
    12541 actaagttta aaaatagatc ggagtaatgg ctatgctttt taatgagaca caattatggt
    12601 ttaaatttga tccttcgaat agatttatca aagattttta taaggtgtgg gattcagaag
    12661 ttttctttct agcaatcgaa gatagcttat taatcaatct ctactattct aataagaact
    12721 actttaaaat ccctgctgca aaaactagaa tgaagaaaga tgtatacttt tt
//
LOCUS       DACDIE010000066        12738 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.066, whole genome shotgun sequence.
ACCESSION   DACDIE010000066 DACDIE010000000
VERSION     DACDIE010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12738
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.066"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="IV250_002250"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="IV250_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP8769500.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="IV250_002251"
     CDS             complement(322..891)
                     /locus_tag="IV250_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAP8769501.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="IV250_002252"
     CDS             1077..1979
                     /locus_tag="IV250_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8769502.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="IV250_002253"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="IV250_002253"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAP8769503.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="IV250_002254"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="IV250_002254"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAP8769504.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="IV250_002255"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="IV250_002255"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAP8769505.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="IV250_002256"
     CDS             8547..8732
                     /locus_tag="IV250_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HAP8769506.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="IV250_002257"
     CDS             complement(8794..9990)
                     /locus_tag="IV250_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP8769507.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="IV250_002258"
     CDS             complement(10160..11596)
                     /locus_tag="IV250_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP8769508.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="IV250_002259"
     CDS             11889..12698
                     /locus_tag="IV250_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8769509.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3803 a         2673 c         2250 g         4012 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctggtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcat gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt gacaattt
//
LOCUS       DACDIE010000067        12590 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.067, whole genome shotgun sequence.
ACCESSION   DACDIE010000067 DACDIE010000000
VERSION     DACDIE010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.067"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            47..899
                     /locus_tag="IV250_002260"
                     /pseudo
     CDS             47..899
                     /locus_tag="IV250_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            996..2882
                     /locus_tag="IV250_002261"
     CDS             996..2882
                     /locus_tag="IV250_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8769510.1"
                     /translation="MGKYQELARKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAADGMIRGLTAFLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFETPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEADETDDRQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMSLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVINYMDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(2923..3825)
                     /locus_tag="IV250_002262"
     CDS             complement(2923..3825)
                     /locus_tag="IV250_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP8769511.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            4055..5458
                     /locus_tag="IV250_002263"
     CDS             4055..5458
                     /locus_tag="IV250_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP8769512.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            5458..6891
                     /gene="ascB"
                     /locus_tag="IV250_002264"
     CDS             5458..6891
                     /gene="ascB"
                     /locus_tag="IV250_002264"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP8769513.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFPV
                     ITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            7036..7962
                     /gene="trxB"
                     /locus_tag="IV250_002265"
     CDS             7036..7962
                     /gene="trxB"
                     /locus_tag="IV250_002265"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAP8769514.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            8176..9903
                     /locus_tag="IV250_002266"
     CDS             8176..9903
                     /locus_tag="IV250_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAP8769515.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     AIIQKMTEE"
     gene            9976..10269
                     /locus_tag="IV250_002267"
     CDS             9976..10269
                     /locus_tag="IV250_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP8769516.1"
                     /translation="MPDTQKTKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAITQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
     gene            complement(10364..11224)
                     /locus_tag="IV250_002268"
     CDS             complement(10364..11224)
                     /locus_tag="IV250_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAP8769517.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            11316..11744
                     /locus_tag="IV250_002269"
     CDS             11316..11744
                     /locus_tag="IV250_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP8769518.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            11906..12082
                     /locus_tag="IV250_002270"
     CDS             11906..12082
                     /locus_tag="IV250_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAP8769519.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            12109..12555
                     /locus_tag="IV250_002271"
     CDS             12109..12555
                     /locus_tag="IV250_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAP8769520.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
BASE COUNT         4119 a         2170 c         2775 g         3526 t
ORIGIN      
        1 tttttgtttt ggatacagcc ctatatatca tggaaaggag caaaacatgc tgattgagaa
       61 agtactgaat aacaacgtcg taataaccag ggatgaaaga aatcaggaaa tgatcgtgat
      121 gggaaaaggt ttggcattca aaaagaaagt gggtgacccg atttttgact catgcatcga
      181 caaaaaattt cagttgacaa atgacgggct ttcacaacag ttccaagaat tggcacaagc
      241 gattcctctt gagtacttag aaatttcttg tgaaatcatt gaatatacaa aagaggctct
      301 acaagtccag ttgaatgatt ctgtgtatat tgcattgacg gatcatttgt atacggctat
      361 cgaacgtcac aaaatgggaa tatttgtacc aaatgtattg ctgtatgata tcaaacaact
      421 atttcctaaa gagtatgcca tcgggaaaaa aaacagtgaa gaaaatctcg gataaatacc
      481 acatcaagtt atcagaagat gaagccggat ttatcgcatt gcatttagtc aatgcacaag
      541 gcattcaaag ccaacaagac aatacctacc tgttcactat aacaatacag gatattctaa
      601 atatcgtccg ctattactat aagattacat ttgatgaaga ttcagtccat ttttttcgat
      661 tcgtcacaca cttacgtttt tttctgacac gcgtcctaga tggagaaaaa tcagaagtgg
      721 gagaagtttc tgaggaattg tttcaggtta ttcaaaaaaa atataaggat acgtttggtt
      781 gcgttgagaa aatagcagac tttttagccg agacgttcaa ctaccagatg tccacggacg
      841 aaaaaatgta tttaacgatc catactgcgc gtttagtgca tgagaatcgt tgtaaataaa
      901 taaaaaaagg aggataaatt aacaagaacg tatgaaataa gggtagtgac attcagttgg
      961 ctaaaccttc cactaataaa ttggagggaa tagaaatggg aaaataccaa gaattagcga
     1021 gaaaaatcgt agaaaatgtc ggcgggaagg aaaatatcaa tggtttgaca cattgtatca
     1081 caaggcttcg tttcaaatta aaaaacgaag aaaaagcaaa tgatgagatc ttgaaaaata
     1141 tggacggtat tgtaacggta atgcgagcgg gagggcagta tcaagttgtc atcgggaatc
     1201 atgtgccagt cgtgtttgaa gaggttataa aagcaggaaa ccttactttt gatgaggctg
     1261 tttcaacaga aaaaatgaga ccgtttgata tgcttattga tggaatatcg ggctgtttcc
     1321 aaccattcct tgctatccta gctgctgacg ggatgattcg tggactgaca gcattccttg
     1381 tcttcttagg cgcgtttgac cgcggttctg gcacatttgt tatgtttgat aatatcggtg
     1441 attctgtatt ccaatttatg cctgttatta tcggtttaac agctgcccgt aaatttaaag
     1501 taaatgaatt cgtagggatg ttgcttggcg cagcattaat gaatcctagc ttgtcgctgg
     1561 aagctttgag tggtgcagca gaagcaccac taacgaccat attttctggc actatttttg
     1621 aaacacctat ttatcaaaca gtattcggca ttccctggat tgcgcgtaac tacgcttctt
     1681 ctgtcattcc cattatcttt attgtgttgt tagctagcca aattcagaaa ccaattaaaa
     1741 agttagtacc tgagatgatc gcgaatttct tcgttccatt cttcaccgtt cttattacaa
     1801 tgcctttagg ttttttactc gttggaccag tattcacatt cgcaactgat attttaatgg
     1861 ctggatttga aacactttta gcactttcgc cagttattta tggagcaatc gtaggcttct
     1921 tctggcaaat tctggtgatg tttggtctcc attgggcaat cgtaccaatg ggattgatgc
     1981 aattttctgt gaatggttgg caaaatatta tgacacctgt tgcagtagtg tcttttggac
     2041 aaactgcagc acttaccgca ctctacttta agcttagaaa tccaaaagac aaagcaattg
     2101 caataccagc cattgtttct ggtattgtgg ggattacaga gccagctatt tatgggttta
     2161 cattgccacg taaaaaaata tttattttta cctgtgtcgg tggtgcgtta gggggcgcgt
     2221 atagtgggtt aatgaattta acttcttgga atcaaggtgg actaggcatc tttacaattc
     2281 ccaactatat tagaccagat ggtgatttaa cagatgtgat taatgtattg attgggatcg
     2341 ccattgcgat ggtggtatcc tttgcgctga cttttttctt ttggaaggag gaagcagatg
     2401 aaacagatga tagacagaaa agatcaggta aagaaattgt aaaaacaccg atacaaggac
     2461 aaatcgctcc actgaatgca gcaaaggatg cagcttttgc acaaggaaca ctaggtcgag
     2521 gaatcttgat ctaccctgaa aaaggagaag tacgtgcccc ttttgacgga acagtcatgt
     2581 ccctgtttcc gacgaaacat gcaatcggga tggtttctga agcaggtctg gaattgctga
     2641 ttcatgtcgg attggatact gttcagttgg aagggaaata ttttgaaagc ttggtgcagc
     2701 aaggagaaag agtgaaacaa ggcgatctgt tgcttcgctt cgacaaaaag aaaatcgaag
     2761 aggaaggata cagtttagaa acacctgtga ttgtcatcaa ttacatggat tatttggata
     2821 ttttggaaaa tcaaggggaa acggtcgggc cagacgatgc acttatcacc gcactgacct
     2881 gaacggttag gtgaaagaat ggactaggct taactattct ttttagtcca cttctttatt
     2941 ttcaaaattg aaaggctctt tcatgatggc tgagctgata gggcgtttat caaatttttg
     3001 tcctacagaa attagatgga ctaaattctg ttgataattt tctgcaaaaa cacaactata
     3061 taacacgtct agaagataac aaatcgatgt attgatcgta aaattaccaa ttttcgaata
     3121 cagtcgttcg cgtgtcgtaa gtcgcaaagc ggctgtactc attgaagata gcgttgaatc
     3181 accgatactg gtcaaagcta aaattggcac atgattttgt ttcaatattt ctgcggtacg
     3241 aaggatcaca ttgttttcac ccgtataaga aatcagaatc ccacacatat cacttgtgca
     3301 attgtacgct tcatagaacg attcaccaaa agtggtgaca atcgtgacat ccattttgat
     3361 gcgcttcatt ttcagcatga aatcttgaga aatcaaagta ttcgagttac tggcgaaaat
     3421 cacgattttt tttgcttgaa gcattagttg tttggctttt tgcagttggt catgatgaat
     3481 gagagaaagt gtatcttgaa tcgtcatctg ttctaaagaa gccattttat ttgcgatcgt
     3541 cataataccg tctgtggtgg aaaaaggaag attcgcatct acgtcttgaa aattcccttc
     3601 aagataggct gtttcagcta aaaaagcatt acgaaaactg acccagccat caaaacccat
     3661 tttttttgct acacggatga gtgtggacgg gtggacataa catcgctcag caatctcttg
     3721 gatggtcatt tcgccaagtg ctggagaatc gcttaagaga aactgaatca cttcattttc
     3781 tgaaggagaa aaggagattt ctttgatttt ttcacgtaat aacatgtaga taccttcttc
     3841 ttgttttttt acgtaaatta taactttttt gaaaatgatt tgtaaacgga tttatgttat
     3901 atagcttaat aaagacattt tttgacaaga tatttacaaa aatccagaag aaaacactaa
     3961 cttttgtaaa tgttactgga aagaaacgaa tttctattgt ttgttttgtt tacaaaaaag
     4021 ataataaaag tgtaaaaagt aaaggagtga gaacatggca aaaggaatca aaatcgctac
     4081 gattggtgga ggcagcagtt acacccccga gttgatggaa ggatttatca aaagatatga
     4141 ggaattaccg atacgagaaa tctggctcgt agatgtagaa gaaggaaaag aaaagcttga
     4201 gatcgtcagt cagatgtctc agcgtatgtg ggatgcttct ccgtatgaag tcaaaatcta
     4261 tgcaactctg gatcgggaag aagcattgaa agatgcggat tttgttacga ctcagtttcg
     4321 tgtcggtcaa ttagatgcac gaatcaaaga cgagcggatc cctagttatt atgggatgat
     4381 tgggcaagaa acaaatggtg ctggcgggat attcaaagct tttcgtaccg ttccagtggt
     4441 tttggcaatc gttgaagaca tgaagcgttt atgtccaaat gcttggctga ttaatttcgc
     4501 gaacccaagc gggatggtga cagaagcagt tatcagatat ggcaaatggg ataaagtgat
     4561 cggattatgc aatgtgccag taatggcgat gatgacagaa ccaacaatgc ttggcaagaa
     4621 aaaagaagag ctgatctaca agtttgccgg tctaaatcac ttccattggc acaaagtagc
     4681 agataataaa ggaaatgacc gcacggcaga attgattgat cgtttatacg atgaagacaa
     4741 tggattgccg aaaaatatct ttgacatccc gtttttcaaa gaacaattaa aacaaatgaa
     4801 catgatccct tgtggctacc accgctatta ctaccgtgaa gaagaaatgc tggaacatgc
     4861 tttagaagaa tatcaaacga ttggtacaag agcgcagcaa gtgaaagaaa cagaagcaga
     4921 gctatttgaa ttatacaaag atccagaatt agaccataaa cctgaacaat tgcaaaaacg
     4981 aggtggcgct tattattcag acgctgcctg tgaaacgatt gcttcaatct atggaaataa
     5041 agaaacacag atcgtggtat ctacaaagaa tgatggagct gttcctgacc tgccggcaga
     5101 ttgtgtagta gaagtcactg cctatgttgg cgcacaagga gcgagaaatg tagcgtttgg
     5161 ttctcttcct gctgctgaaa gaggctggtt gcaagtaatg aagaatatgg aattgctgac
     5221 gatcgaagca gctgtgactg gtgattatgg gacagcttta caggcgttta cgatcaatcc
     5281 gcttatacga tcaggaaaga cagcaaaacg gatcatggat gagttgttta ttgcacacca
     5341 gcagtattta ccgcaattca aggaaacaat cagccggtta aaagcagaag gaatcgaagt
     5401 ccaagatgaa ctggcaagag aattatcaag caatacacat gtaatggagg aagtatgatg
     5461 ggattcagaa aagatttttt atggggcgga gctacagcag ccaatcaatg cgagggcggt
     5521 tatgatgaag gcggacgtgg gttagcgaac gtagacgttg tgccaatcgg agaagaccgt
     5581 tttcctgtca ttactggaaa aaagaaaatg tttgaatttg atgaaaatca tttttatcca
     5641 gcaaaaattg cgatcgatat gtatcatcat tataaggaag atattgcttt atttgcagaa
     5701 atggggttca agacttatcg gatgtccatt gcttggagtc gtatttttcc aatgggcgat
     5761 gaaaaagagc caaatgaatc tgggttgaaa ttttatgagg atgtctttaa agaatgtcaa
     5821 aagtacggga tcgagccgct tgtgacgatt acacattttg attgtccgat gcatttgatc
     5881 aaagaatatg gtgcgtggcg ttccagagaa ctcgttggtt tttatgaaaa tctgtgccga
     5941 gtcatcttca atcgctataa aggacttgtc aaatattggt tgacattcaa tgagatcaat
     6001 atgatccttc atgcaccatt catgggcgca gggctgtatt ttgaagaagg agaaaatgaa
     6061 gaacaaatca aatatcaagc ggcacatcat gagttggttg caagtgccat cgcaacgaag
     6121 attgctcatg aagtagatcc ggagaacaaa gtaggctgta tgctggcagc aggagcatat
     6181 tatccataca cttgcaatcc tgaagatgtt tgggcttcta gaaaatcaga tcgagaaaat
     6241 tatttcttca ttgatgtcca gtcacgagga gaatatcccg cttatgcatt gaaagaaatg
     6301 gaacgaaatg gcattcagat cgaaatggaa cctggggatg ccgcattgct gaaagaacat
     6361 actgttgatt ttatctcctt ttcttattat tcttctcgag tttcgactac tgacaacgaa
     6421 ctcttggaac agactgcagg aaatatcttt gcttctgtca aaaatccata tctggaagca
     6481 agtgaatggg gttggcaaat tgatccatta ggcttgagaa taacgatgaa tgatatttat
     6541 gatcgttatc aaaaaccact atttattgta gaaaacggct taggtgcagt cgatacgccc
     6601 gatgagaatg gttatgtgga agatgattat cgaatcgact atctggcagc acatatccaa
     6661 gcaatgaaag atgcagtaga attagatgga gtcgatctat taggctatac gacttgggga
     6721 tgtattgatt tagtatcagc tggtacaggt gagatgaaga aaaggtacgg attcatttat
     6781 gtcgatcgtg acaacgaggg aaatggcaca ttagcccgtt cgaagaagaa atcattcaac
     6841 tggtataaaa aagtaattga ttcaaatgga gaaaatcttt cgaatgattg atcaatgatt
     6901 tataaaagtc tattgttttt cagcggattt ttcgagtata ataaagctat tcgagaaacg
     6961 caagcagaac aatagacttt ttttcgcatt ggctgtaaag tatgcttact tgattaagta
     7021 aaggagaatc tacatatgta tgatgtaatc gttattggcg caggtcctgc tgggatgact
     7081 gctgctttat atgcttcacg ttcaaactta tccgtattga tgatcgaaca aggcgcccct
     7141 ggcggccaaa tgaacaatac ggcagaaatc gaaaactatc ctggtttcga ttcaatcatg
     7201 ggtcctgatc tagcttataa gatgtatgaa ggtgtttcaa gatttgggac agaaaatgct
     7261 tatggaatcg tgcaagatat caaggatcac ggcagccaca aagaagttat ttgtgaagat
     7321 aaaacttacg aaggaaaaac agtcattatc gcaacaggct gtatccaccg taaattaggc
     7381 gtacctggcg aggaagaatt tgctggacgt ggcgtttctt actgtgcagt ttgtgatggt
     7441 gcgtttttcc gtaacaaacg tctagtagtg atcggaggag gagactcagc agtagaagaa
     7501 gccatctatt tgacacaatt tgcttctgaa gtagtcatcg tccaccgtag agatgaatta
     7561 cgtgcacaaa agatcatcca agaccgtgca tttgcgaatg aaaaaatttc ctttatgtgg
     7621 gatacagtcg ttgacgaaat cgttgggaat gaaatggtag tgacaggtgt gaaagcacgt
     7681 aatgtcaaaa ccggcaatga gtttgatttg gcagcagacg gagtattcat ctatgttgga
     7741 ctggatccat taactgcacc atttagagaa gcagggttga cgaacgaagc tggctggatt
     7801 ccaacagaca acgaaatgaa gacgaatata ccaggcgttt ttgccatcgg agatgttcgt
     7861 gaaaaagatt tgcgacaaat cactactgct gttggtgatg gtgggatcgc tggacaacaa
     7921 gtgtataaat acttggaatc tttagaagaa acaactgcat aagtttaaaa agagtggaag
     7981 aacggtttca attgttcttt tactcttttt tttcgcgtga aatctactga atccgattta
     8041 tgcgaatact tgcaaccgtg cttgataaaa gaatcctttc ttgatttaag cgttttttgg
     8101 tcctcatact tttatatcta ataagtgaat gctatactta ttaagaacca tatttcagaa
     8161 agaggtgtta ttttcatgtc ttgggaacaa gtgtatgaac aatgggtaca tgaagaaaat
     8221 ttagaagaaa acttaaaaaa acaattaaat gatctgggtg aagatcctga aaaacttgaa
     8281 gatgcttttt atgcaccgtt ggaattcggt actgcaggca tgcggggaat cttagggcct
     8341 gggatcaatc gaatgaacat ctatacggta agacaagcaa cagaaggact tgcacgtttc
     8401 atggatacac aaggcgaaga aacaaaacgc cgaggtgtcg ctatcgctta tgactcacgc
     8461 catcaatcac cagaatttgc catggaagca gccaaaacat tagcacatca caatatccca
     8521 tcttatgtat tcgaaagtct acgtccaaca ccagagcttt cttttgcagt ccgtcatttg
     8581 aagacgtttg cgggaatcat ggtaactgca tcccacaatc cagcagctta caacggatat
     8641 aaagtctacg gcgaagatgg gggacaaatg cctcctgcag acgcagatgc attgacgaaa
     8701 tatgtccgtg aagtcagcaa tccgttaaaa gttgaagtat tatctgacga agaagctgaa
     8761 catagcggat tgatcacgat catcggagaa gaagtggatg ccgcttatct agaagaaatc
     8821 aaagcagtaa cgattgatca tgaattggtg gaaacaatgg ggaaagattt gaaacttgtg
     8881 tacacaccac ttcacggaac aggaaaaatg ttgggcgaac gtgcgttgaa acaagctgga
     8941 ttcgaacaat tcgtactagt tccagaacaa gctgtcgcag acccagattt cactacagtc
     9001 aaatcaccaa atccagaaga acactctgct tttgagtatg cgatacgact tggcgagaaa
     9061 gaaggcgctg atttgctgat tgcaacagat cctgatgcag accgtttagg tgcagccgtt
     9121 cgtttgccaa atggctccta tgaagtattg accggaaacc aaatcggagc actgtttgct
     9181 caatatattt tggaagcaca ccaatcgcga ggcactttac ctgaaaacgc agcggtattg
     9241 aaatcgatcg tttctagcga attacctaca gcaattgcgg aacactatgg ggctaccatg
     9301 ttcaatgtat tgacaggatt caaattcatt gctgaaaaaa ttcaacaatt tgaagaagat
     9361 cattcccata cttttatgtt cggattcgaa gaaagttacg gttatttagt taaacctttc
     9421 gttcgcgaca aagacgcgat ccaagcattg ctcttattag cagaagtagc agcttactat
     9481 aaaaaacaag gcaaaacgtt gtatgacggc ttgcaaacga tttttgaaac ctatggctac
     9541 tacgcagaaa aaacgatttc tgtgacaatg agcggacaag aaggttctgc gaaaatctct
     9601 gctttgatgg aaaaattccg tgaagaagca ccaacagaat ttgccggagc aaaagtcatc
     9661 caaacagaag acttcaaaga gttgacgaga acagaccgtg aaggaaacgt agaaaaacta
     9721 acgacaccgc catctgatgt attgaagtat acgctggaag aaaatggctg gatcgctatc
     9781 cgtccttcag gaactgagcc gaaaatcaaa ttttacatcg gtgtaaaagg aaactcttca
     9841 gaagacgcag ctgaaaaagt agccgcttat gaagcgatta tccaaaagat gacagaagaa
     9901 taaaaaaact tgagactgat ggaaaaagtg gtaaacttta gaagtcagtc ttttttcaag
     9961 ttggaggtga atggattgcc agacacgcaa aaaacaaaag tagagaaagt cagccaacta
    10021 ttcaaaatgt tgagtgaccc cactcgtctc aaaattctgc tgtatctgaa agacggggaa
    10081 caaaatgtga cagcaatcac ccaagcagtg gaaatggaac aatctgctgt atcgcatcaa
    10141 ctgcgtttat taagagaaaa ccatgtcgtc aagtcccatc gtgagggaaa agcgattttg
    10201 tatagtcttg atgattctca cgtactggat attttgaacc agacactcaa acatgtggaa
    10261 catcaataac gataggtgca aatagagaca aactggactg tgtcaacgaa tcttcttcgc
    10321 tgacacagtc cagttttttt taagaaaaag acaagaaaaa ctgttagtct gtttcttcgg
    10381 tggtttcagt tccataataa ctatgatctt ctaggtctaa agcattcaaa ataagcgaag
    10441 ccgtttcttc aatagataaa gatgccacat tgatcactac acatcctagt ttttcataaa
    10501 gatcattcgc aaaatcgagt tcagctctaa ttttttcgat atcggaataa gcagtatcag
    10561 ggtttaatcc gtaagagcgc atccgttctt tacggatatt atttaaaata tctgggttat
    10621 tcgttagtcc cacgattttt ttaggatcga tttcccacag ctgtttggga atatgtgctt
    10681 ggggaatcaa aggtaaatta gcaactttta aatttttatt tgctaaaaac aagctcaaag
    10741 gcgttttgga agttcgggat acgcctagca aaacgacatc tgcttctaaa aaaccacgag
    10801 gatctttccc atcatcgtat tttacagcaa attccattgc tttgatccgt ttgaaatagt
    10861 tctcattcaa taaatggagg gcaccaggtt gccgaattgg cttgatacca gttaaacgtt
    10921 caatttctga tatcggtggc gtcatcacat cgaaactaaa taattgatgt tcttcacaaa
    10981 aagatcgtgt tatctcgatc agttcttgat tgattaatgt atgcaggaca agtgcttttt
    11041 cttctaatgc atcttgcaat gcctgcctta atgttttttc atctgtcacg aacgttctgc
    11101 ggaataggtt gaactcaacg gtaggatact gagccatagt agcttgcgct agttttgtcg
    11161 cagtttctcc agcagagtcg gagatgacaa aaaaagtaac gatttctgta gaatcggaag
    11221 acatggataa aacctcactt gcattttttt tatgtttatt ataccatact ctagaatgcg
    11281 tacttcgtaa tcattttgat ttggaggaat aagtcatgaa aaaacagaca ttgatcgaac
    11341 aatcattaga aacattgaaa aataacggtt ttaaatatac taaaaaaaga gaagcattgt
    11401 taacttattt agtgaagcgt aaccgctacg tatctgctaa agaagttttt gactatatga
    11461 atcaggaatt caaaggggta agttatgata cgatttatcg gaatctccat gattttgcag
    11521 agatcgattt actggaagaa accgatttaa atggagaaat gaagtttcgt tttcattgct
    11581 gtcaggggga aatcggccac caccatcatt ttatctgtac agtgtgcgga acaacgaaag
    11641 aaatccatat gtgtccgatg aattatttcg aagaacagct aggtggatgc acgatcgaag
    11701 ggcatagatt cgagattttc ggaagatgtg aaaattgtca ataaggattt aaataaaata
    11761 acatttgata atttaaaaaa aaagcaagat ttccttgacg gtcaaaacag cattagatat
    11821 aatatttaca ggacagtctt gttttttgag atatatttca ataacaatta cggttacttt
    11881 ttaagctcgg agggagggaa ttaacatgtc aaaaacagtc gttcgcaaaa acgaatcact
    11941 tgacgatgct cttcgtcgct tcaaacgttc cgtgtcaaaa gcgggtactt tacaagaatc
    12001 tcgtaaacgt gaattctacg aaaaaccaag tgtaagacgc aagaaaaaat ctgaagcagc
    12061 tagaaaacgc aaaaaattct agttctgtga tgaattggag ttggtagtgt gtcactacta
    12121 agtagattaa acgatgacat gaaagcagcg atgaaagcga aagataagga atctttacaa
    12181 gtcatccgaa tgatcaagtc atctatacaa aacgagcaga ttaaagaagg tcacgacttg
    12241 acagaagaag aagaactaac ggtgttgtct cgcgaaatga aacagcgacg tgattctttg
    12301 catgagtttg aagaagcagg acgtgacgat ttagctgaaa aagtaaaaag tgaaattgta
    12361 atagtcgaaa aatatatgcc ggaacaactt tccgacgaag aaattcgaca acttgttcaa
    12421 gaagcaatca ctcaaactgg tgcttcatca atgaaagaat ttggcaaagt tatgggtgca
    12481 attatgccta aagtcaaagg aaaagcagat ggaaaccaag tgaatgcgat tgtgaaagaa
    12541 ctattacaat cttaatttta tcatttaaac tgcggacagt gattgtccgc
//
LOCUS       DACDIE010000068        12572 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.068, whole genome shotgun sequence.
ACCESSION   DACDIE010000068 DACDIE010000000
VERSION     DACDIE010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.068"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(60..1766)
                     /locus_tag="IV250_002272"
     CDS             complement(60..1766)
                     /locus_tag="IV250_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAP8769521.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            1956..2579
                     /locus_tag="IV250_002273"
     CDS             1956..2579
                     /locus_tag="IV250_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769522.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            3029..4078
                     /locus_tag="IV250_002274"
     CDS             3029..4078
                     /locus_tag="IV250_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8769523.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            4075..4956
                     /locus_tag="IV250_002275"
     CDS             4075..4956
                     /locus_tag="IV250_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8769524.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            4959..5729
                     /locus_tag="IV250_002276"
     CDS             4959..5729
                     /locus_tag="IV250_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8769525.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            6008..6862
                     /gene="aroE"
                     /locus_tag="IV250_002277"
     CDS             6008..6862
                     /gene="aroE"
                     /locus_tag="IV250_002277"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAP8769526.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            7040..8203
                     /locus_tag="IV250_002278"
     CDS             7040..8203
                     /locus_tag="IV250_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAP8769527.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGGQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            8320..8730
                     /locus_tag="IV250_002279"
     CDS             8320..8730
                     /locus_tag="IV250_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP8769528.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            8904..11132
                     /gene="topB"
                     /locus_tag="IV250_002280"
     CDS             8904..11132
                     /gene="topB"
                     /locus_tag="IV250_002280"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP8769529.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            11270..11587
                     /locus_tag="IV250_002281"
     CDS             11270..11587
                     /locus_tag="IV250_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769530.1"
                     /translation="MKKVHSFLNYLMGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAY
                     SPTLILSTLTAGAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQ
                     YFG"
     gene            11747..11983
                     /locus_tag="IV250_002282"
     CDS             11747..11983
                     /locus_tag="IV250_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP8769531.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            11980..12379
                     /locus_tag="IV250_002283"
                     /pseudo
     CDS             11980..12379
                     /locus_tag="IV250_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
BASE COUNT         4230 a         2118 c         2638 g         3586 t
ORIGIN      
        1 ttataactaa ttgctgttgt gggaaagtac gcaaatatga ttctagaaac actcattgat
       61 tacctgtttt gtttcatagc tgtaattttt tcttcttctg gtgtcacgat aatcgtcttt
      121 tggttttcat aaataacata accaggcttg gcgccatttg gttttcggat atgctttacc
      181 tgtacaagat caaccggcac ttgggcagag tatcggtatt ttgaaaaata ggcagctaac
      241 tcggcagctt ctgtaattgt ttcatctgag ggcttgtcac ttttaatgat gacgtgcgaa
      301 ccgggtatat tttttgcatg aagccaatag tcggtttttt tagctgtctt cattgtcagc
      361 tggtcatttt gaagattgtt tttaccaaca agaatgagcg ttccgtcaga agaaaagtat
      421 tgatcaggct gagatggttt cttacgtttt tgttttttta agttcttctt tttcaggtat
      481 ccttctgaag tcaattcttc tttaattgct tcgatatcca ttgggccggc aatttccagt
      541 tgagataaga cagattcaag atattggatt tcatctttgg cttcttggat ttgttcgcca
      601 atcagcttaa cagcgttctt cagcttttgg tatcgatgga aatatttctg tgcattttga
      661 tttggtgtca atgccggatc tagtgctatt ttgattggcc gatcttcttc ataataatta
      721 ggtaaaacaa cttcttttgc gccgcgtggt acttgtgtca gaaaagtagt aagtagttcg
      781 ccatttctac ggtaattctc agcgttttcg gattctttga gtgtttgttc acgttttttc
      841 aatttatttt tatttcgttt tagttcattt tcgatttttc gaatcagttc tccgccttgt
      901 tgttttgccc gatctttctc tgctttttcg tgataatagc tatctaataa ttgactgagt
      961 gttggataag ctgttacagc agatgcctgt tcggacaata cctgataagc aatcggtgtg
     1021 aaaaaatctt tattttccgt ttcgtaaaaa gtaggaatag gctgatggct gatggctgag
     1081 aaaaattcgt cccatacaac catcttttca ttaggacgtt ccagtaatct tttggtcagc
     1141 tcttgagcga tatcgaaacc gattccctga aactgacgtt ggattgcttt tggatctagt
     1201 tcagtttggg aaagccgctg aaagattttt tctttctcgg agctaaatgg gttcaagacc
     1261 tcttgttttg gcggagcaac atattctaca ccaggaagca aagaacgata agtgttttga
     1321 gagcttccaa tgtgtttgat cgcatccagt atttttccag tttctcgatt aactaagacg
     1381 atcgtactat gacgtcccat taattcaacg atcaatatga tattttgtag atcacccaac
     1441 tcgtttcgtt tagcaaagtg gaaatggatc actcgatcat tttcgatttg ttcgatgtct
     1501 tctaaaatag cgccatccaa atattttcga agcatcatca cgaagttagg cggattatca
     1561 ggattctgat aatcgatttg ggtaatctgt acacgagcat aacttgggtg cgcagataat
     1621 aataatcgat ggtttttgcc gcgtgaacga atcaccagca cgatttcatt ttcataaggc
     1681 tgatggattt tcgagatccg gccagatagt aacgtttcac gcaattcgtt gaccatcgcg
     1741 tgggtaaata caccatcaaa ggacatggtt cattcctcat ttcttttcat aagtatattc
     1801 attataacga aaaaaagaag aaaaaccaat ttggccgaat agtaaagcaa aagtctagaa
     1861 aaatcttgga ctaattacat aaaaaacagt gtttctatcg ttgaaatcca aatttttagg
     1921 tataataaaa aataggagaa aaagtagaaa agaaggtgtt tgttatttgg gaagtagtta
     1981 aatcgagagt acaagaaatg aatgaacatt tctcagtgtc attcgcagaa aatccaagta
     2041 agtattttcg acgtcttgtt tttctgctca tcttatcatt gctgttaaca gttgcaatgt
     2101 ttttattgcc ggaaaaaatc cgagaagagc aagtaaatag tctcgtttat tcgtccatac
     2161 caaccagcaa attgaaacct tttgattaca gcaaagctga tcagaaaatc aaacaaagcc
     2221 gggcgatatc ggtgatgttc agcaaaccga acgggaaaac ctatcaggac atgcttgatg
     2281 tttttaatga tcctgagctt atggaagaat tgaatcgacc gatcttttat tatccgatcg
     2341 tttatgatgt acatgaactg gaacaaaaat ataacatacg aacggatgaa gtgacattta
     2401 ttttctttga tggaggaaaa gaagcaaatc ggataactgc gggtaaggga ggaataactg
     2461 attttgataa ggagttgatc cctgaactga atcgacttcc gttagccaat atcaaacaat
     2521 tagaagaaga attggataga acgggagtaa cttcccaaaa ttccgagatt gctaattgag
     2581 acattggcgc gatgaaacgg tgggtctata tagcgctatt tttaattatc agaacaagtg
     2641 atgtttgatc ttcaaaataa caaataaaaa attaaaaaat atagttgacg aaattcaaaa
     2701 attcgattag acttaaatca attcttaagg aaagggtgac ctgtaatgaa cttatcaaaa
     2761 cgtatttcag cagaaaacaa aaaaaattgg cgcagctggc gtagataagt tgtattaatg
     2821 gtgagacttt cccatagata caacttttta gagtgcattt ctaaagcttg tatctatgct
     2881 agacatgaac attgactgcc gcatagatta gaatctatgc ggttttttgt gtaaagacaa
     2941 tgaccgcttt ggccattgtc tttttttgta gactggatac ttgcctgatt ataataaata
     3001 aaaagattga ctttgcagga ggaaaaaaat gaaaaacaaa cgattaataa cagttgtagt
     3061 aattatttta gtttatttag caggtgcatt tatttatgga aaagcctcag gcgaaaaaga
     3121 aagtacttcg acgaaccaga caaaaaaaac aacagttgtt ggggtattgc aatacgtgag
     3181 ccatccggca ttagatgaga tttatcgagg aatcaaagat ggtctgaaag aagaaggatt
     3241 aaaagaagga gaaaatcttg agatcaaatt tcaaaatgga caagccgatc agagcaagct
     3301 agccacaatg agtcaacagc tagtacaatc tgatccagat gccttgattg gaatcgcaac
     3361 acctgccgct caatcattag caaacacgac ttcatcgatc ccagttgtgt taggtgctgt
     3421 aacggatcct gtgggggctg gattagtcgc ttctttagag aaacctggtg gtaatgtaac
     3481 aggtgtttct gatcagccgc ctgttacttc acaaatcaag cttgcttctg atttgttgcc
     3541 aaatgcaaaa aaagtcggaa tcttgtatgc ttcttcagag gataattcaa aataccaagt
     3601 agcacaagca gaagaagctg caaaaaaaga agggttagaa tcagtaaaat atgcggtgcc
     3661 ttccactaat gagattgcac aaacggttca agtgatgagc cgacaagtcg attttatcta
     3721 tgttccatta gataatacca ttgccaatgc gatgcaaaca gtggtaaaag aagcaaacaa
     3781 agcaaatatc cccgtcattc catctgtgga tacaatggtg gaacaaggtg gactagcaac
     3841 aatcgggatc aatcaatatc aattagggct tcaaagcggt aagatggcgg cagaacttgc
     3901 gagtggaaaa gaaaaaccgg aaacgacgcc tgtttataca tttgatcaag gggatacagt
     3961 gatcaatcaa tctcaagctg atcgcttagg aatcattgtt ccccaatcga tgaaggaaaa
     4021 agccaaaatc attacagatg actcgcagca agaaacatct aaggaggatg acaaatgatc
     4081 gtatcagcta tttcacaagg catgctttgg gcaatattag gattagggat tttcatgact
     4141 tatcggatct tagactttcc tgatatgacg acagaagggt ccttcccatt aggtgctgct
     4201 gtctgtgtga cagctattac tcatggggta gcgccgattg tagctacatt attaggcgta
     4261 ggtgctggca tgatagcagg actcgttacg gggttacttt atacaaaagg aaagataccg
     4321 gttattttag cagggatttt agtgatgtca ggattgaact cagttatttt gtttgtgatg
     4381 cgaacaccaa atctttcttt attgaatcat ccgattctac aagacagctt ccatcgcttt
     4441 gggttacctg attactttga cacagtcctt cttggcttca tctctttggg catcttgatt
     4501 gctgtactgc tattcttctt caatacagat ttgggtcaag gctatattgc gacaggagac
     4561 aacgaaacga tggctcggtc gttagggata aaaaccgatc gtatgaaaat tttaggttta
     4621 actttgtcga acggtgtcat tgcattatcc ggtgcgctga ttgcccagaa cgacggatat
     4681 gcagatgtaa ataaagggat tggcgtaatc gtcatcggtc ttgcttccat catcatcggt
     4741 gaagtgatct tccaagaatt gacgctagca gaaagattga ttgctattgt tgtcggcagt
     4801 atcatttatc agctattgat tttagtagta atcaaactag ggtttgacac gacttatctg
     4861 aagttgttct ccgctatcat cttagctatt tgtctgatga tcccgcaatt gaagaaagca
     4921 ctgaacttaa aaacgggttt tgagaaggag gcatagtaat gactgatata ttgacaatca
     4981 aaaatggcag aaaagtcgta aacaatggaa tgaatgaaga gaaagtacta ttggatgaaa
     5041 ttaaccttac aatcagagaa ggggacttca tcactgtcct tggcgggaat ggtgctggaa
     5101 aaagcacact gttcaatacg atcgctggta cgcttcaact cagtcaagga tcgatctctt
     5161 ttaaagatca gctgatcaca aaagattcag aagaaaaaag agcacgattc ctttctcgag
     5221 ttttccaaga ccccaagatg ggaacggctc ctcggatgac agtagcagaa aatttgctac
     5281 ttgcacaaaa aagaggtcaa aaacggaaat tccgtttacg taacttgaaa aatcaaaaag
     5341 aagatttttt cagactatgt caagaagtcg gtaatggcct agagcaacac ttggatactc
     5401 cagcgggtga attatctggt ggtcaacgcc aagcattaag cttgctgatg gcgacgatca
     5461 cagctcctga gttactatta ttagatgaac atactgcagc tttagatcct aaaacagcta
     5521 aagcattaat gcgattaaca aatcagcgga tcaatgaaca aaaattgact tgtctcatga
     5581 tcacgcatcg aatggaagac gccttgaaat acggaaatcg tctgatcgta ctccaaaaag
     5641 ggaaaatcgt tcaagatatt tctgaaaaag aaaagcaaca tttgacaatg ggcgaccttt
     5701 tgcagttctt tgaagaagta gtggattgat ttcgacagaa aaattccttt cagtagaagg
     5761 acttgaaaac tcattgacat cttcctttga ttcatcctat acttaaaaca ataaacaatt
     5821 aaacatattt gggtgttttg taggatcacg tagtcaatga ttttaacaat taaatagaat
     5881 attctttgct ctgacaagta aacgatgaga aaagaatttg ctactttgcc acctagacgt
     5941 ttcgtacttt ttcgtatcaa acgctaagtg gcttttttta ttgaagtaag aacaaaggag
     6001 gaataagatg atttctggac aaaccaaatt agctgggctc tttgctgatc cggcttctca
     6061 cagtttatcc ccattgatgc acaatactgc ttttgaagca aacaacatcg atgcggtgta
     6121 tttagctttc caagtaaatc caatgaattt gaagcaagca gtcgaaagta ttcgaacttt
     6181 tgatatgtta ggtgtaaatc tttcgatgcc gaataaaaca gcggttattc cttatctgga
     6241 tgaggtaagc caggaagcgc aattgatcgg agcagtgaat acgatcgtgc acgaaaaggg
     6301 tcgtttgatt ggctataata ctgatggtgc gggcttcatg caatccgtgc aagaagctgg
     6361 gatttcgatc aaaaagaaaa aaatgactct actgggagct ggaggtgcag caaaggcagt
     6421 cactgttcaa gcagcgttag acggcgctga ggaaatcgta gtttataaac gaaacaacca
     6481 aacattcgct caagtaaaag aaacattcga acaactcagc tatcgaactt cttgtaagat
     6541 aaaagttcgt gactttgccg atacgcatca attaagaaaa gacttgaaag aaagctgtct
     6601 tctagtcaat gcaacagata tcgggatggg aaagaaaatc aatcagtcac cgctcttaga
     6661 tataagcatg atgcatgaga atctagcggt ttttgatttg atttatgcac ctagagaaac
     6721 acgtttatta aaagaagcaa aaaaagtagg agcaaaagga tacaatggac ttggcatgtt
     6781 gatctaccaa ggagcaatcg cgtttgaatt atggacaaag gaaaagatgc cgatccatgt
     6841 gattgaaaag ctgtttgatt aaaaaaggat aatagataaa gatcttttat tctcgcataa
     6901 acggtctcaa gaaaagtaat cctttatgta tcgaagcatt tatttcgtta ttttttatta
     6961 aattatcaaa aaatgatagg cttttgtcga ttggttatcg tatcctttat ttatcagtaa
     7021 gaaacgagga ggaaccacaa tgagtcggat ggatcgctat aaggacatcc ataagaaggc
     7081 gaaacctctg aagcgggata cggggagtat ttttgctaga agagaacgaa aacgaacgcg
     7141 tatggaaaat gaggaagatg ctacccgtat ttatgaaaga gagcagcatt catttgaaca
     7201 tttgaaccaa tacgagcaac agatatacag agatgcacca agagcagaag aacaacaaaa
     7261 aaataaaaaa tttttcaatc ggcaaaagaa gaacaaaccg ttaaaacctc gaagaaagcg
     7321 atcgtggaaa aaaatcattc tatcgatttt gcttgttttg atcctttatt caggtatctc
     7381 attttttatt ggtaaatatg tagctgaaca tgacacaagc ttagaggcag cgacttcaga
     7441 aacctttcat gggcaaaaag cgacgaatgg tgcgcataat attttgatcc ttggaagtga
     7501 tacacgcgga gaagatgccg ggcgagccga tacgatcatg gtcttacagt tggatggtcc
     7561 ttcccataag ccgaaactga tttcttttat gcgtgacagc ttcgttacga tcccaggggt
     7621 aggacaaaat aagatcaatt cagcttatgc atacggagga gccgatctag tgcgtcaaac
     7681 tttagtagaa aattttggaa tcgattgtca atactatgcg aaagtcgatt tcaaatcgtt
     7741 tgaaaaagtg atcgatgcct tattcatgaa cggagtgaag atcgatgcag aaaaagatct
     7801 gaatctggat ggtgtagata ttaaaaaagg gggccagaag atggatggac atgttctttt
     7861 gcagtacgca cgtttccgaa tggatgaaca aggagacttt ggccgtgtca gacgccaaca
     7921 gcaagtaatg aacgctattt tcagccaatt gaagaatcca ttgaatctga tccgtgcacc
     7981 ttatgcagct ggtaaagctt taggatatac atccacagat gtcactgctt ggttcttgac
     8041 gaaaaacctc ttttcgattg cacgaggagc tggtggcatt gatcgaataa gtgtgccagt
     8101 agacggttcg tggaattatg ggcaaagtga ttatgctggc agtattcttg aaattgataa
     8161 tcaaatgaat caatctgcta ttacagattt tttgaataaa tgatcataaa aaagatcaag
     8221 aagaagctgc gcaaggcttt tgtcatgaat caatcaaacg attcactcgt aaaaaacgct
     8281 ttcttatggt agatttaacc aagagaggag ctgtttacat tggatatcta tctgaacttc
     8341 aaaaatgagt cgcgtgaagc catcgctttt tatgaaaaag tttttcagac tgaagcatca
     8401 ggtatcacca catacggtga aatgcctgga atagaacaaa tgaacttgcc agaagaagca
     8461 aaaaatctaa tcacgaatgc acggctgaac atttatgata cattagttat gtttgcagat
     8521 acgccagaag gaattggtcc tgaattagtt gtgggaaata atgtttcttt agtcatccag
     8581 acaagaaatg aagaagaaac gaagcgccta tttgaaagct tagctgaggg tggtaagatt
     8641 gagatgccac tggatgctgt tccgtgggct cagttgtatg ggatggtaac tgatcgattt
     8701 ggcatcaact ggcagattaa ccaagagtga cagctaaaga tgaatatttt ctggaaacat
     8761 caagcagcaa acggatcatc gtttgtttgc ttgatgtttt tgtgtctatt tcgactatac
     8821 ggtatctttt ttttattgcc agatgcgaaa atatgttcca taatagagaa gtccgcaaaa
     8881 aattaagtaa cggaggtcag aagatgaaga cggtgatact agctgaaaaa ccttcacaag
     8941 caaaagccta tgccgaaacc ttccaacagc aaaagcggca tgaagggtat tatgaaatca
     9001 gcgatccatt gtttgctggt gaagtaacca tcacttatgg ttttggacat ttggtagata
     9061 tggttgctcc aggagcttat gaagagcgtt gggcaaaatg gtctttagaa aatttgccta
     9121 ttttcccaga aaccttccgt tacgaagttc caatggacaa acgtgcacaa ttttcgatcg
     9181 taaaacgaga attgcagtca gccgatacca tcattattgc aacagatggt gatcgagaag
     9241 gtgaagccat cgcgtggtcg attattcgac aagcaaacgc attttctaag gaaaagaact
     9301 atttacgttt atggatcaat tctcttgaaa aagaagcaat ctatgaaggt tttaagaatc
     9361 tccaacctgg aaagaactat tttccaaaat ataaagaagc gcaagcaagg cagaacgctg
     9421 attggctgat tggaatgaat ggaagtccat tatatagctt gctacttcaa cgaaaaggga
     9481 ttgatggcag tttttctctt ggtcgtgtac aaacaccgac actctatatg atttatcagc
     9541 tgcaagaaga aatcaaacac ttcaaaaaag aaacgtactt tgaaggagaa ggtttaatta
     9601 cgacaaacaa agggcagttt tctggaaaaa tcgtaccaaa aaaagcattt aaaacccaag
     9661 aagagttaac gaaggaaata gaaaaacttg gagcgcattt aggtaagcaa aacggacgga
     9721 ttcttgaagt tacgaaaaag gaaaagaggt tgcatagtcc acgtttattt tcgctatcta
     9781 gtttgcagac aaagatgaat cagctaatga aagcaagtgc caaagctaca cttgatgctg
     9841 ctcaaggatt gtacgaaagc aagtttctaa gctatccaag aacggattcc ttttacatta
     9901 cagaaaatga gcatcagtat ctggttaaac atctgcaaaa ttataaaaaa tttttaggaa
     9961 tagaagaagt acagacaact gagatcaaag caaagaaacg atatgtcgat gcgaaaaaag
    10021 ttcaagaaca tcatgcaatt attccgacaa agacgattcc gactcctgca cgctttgcga
    10081 agctttccaa attacagcaa gcaatctatt tgcaagtttt gcgcactact gtggctatgt
    10141 ttgctgcaga ttatatctac gaagagacga ccgtaatgac gggcgtacag caattaaagc
    10201 taaaaagcat cggaaagatc ccattacagc aaggttggca ggaaatctta cgcatcaagt
    10261 caaaaaagaa agaagctcag actcttcctc ctatcgtaga aaatgaagaa gcagaagtag
    10321 aactgaaaag tatggagaaa gaaacacaac ctccaaaacc ctataccgaa ggaacgttga
    10381 tcacagcaat gaaaactgcg ggaaaaacgc tggataatga tgaagcgcaa gaaattctca
    10441 aagaagtaga aggaataggg acagaagcta cacgagccaa tatcattgaa aatcttaagc
    10501 agaagcatta catcgaagtc aataaaaacg aaataactgt tacgccaaaa ggaatcactc
    10561 tttgtaaggc agtagccaat gaaccattac tgacgagcgc agaaatgact gcgcgctggg
    10621 aagggtactt gagaaaaatt ggtaaacaag aggggacacc atctgttttt ttagagaata
    10681 tcaaaaaatt catcctacat ttattggcag aagttcctaa tcaaatcgac aaggtagact
    10741 tttcagatga gattagcggg acacgtcaga taaaagcgat ggagaagaaa aacgatcaat
    10801 taggaagatg tccaaaatgt aaaaagggaa tagtcatgct ttatcctaaa atcgctacat
    10861 gcttgaatcc ggaatgcgac ttcaaattgt ggccaacagt agctaaaaag aagctgacta
    10921 aaacgaatct cagagatttg ctttcaaaag gaaagacaag taaagttgtc aaaggtttta
    10981 ctggtaaaaa agggaaattc gatgcagttt tagtattaaa agaagatatg acaatcggtt
    11041 tttcttttcc atggatcgaa aacagtggaa caaaagcagt tgatggaaac aaagaaacat
    11101 caagaaaaaa taatgaaaac gaagtagagt aaatgaaaac aatgaaatag cggaatattt
    11161 attccgaaaa gtgagaatat aaataccaat aaaactaaaa aatatcagtg aatcaagcaa
    11221 aaacgttggg gtaggctgat tttatataga tagcaaggag tttttttatt tgaaaaaagt
    11281 gcattcattt ttaaattatc ttatgggagc aatcacgatt gtggctgtgt accaagcatt
    11341 tctaaagtat cctgattacg ggacgaagtt gttgtcattg acgcacaatc aaacagcata
    11401 ttcaccaacg ttgattttat cgactttgac tgcaggagct aaaagcgcta cttatttttt
    11461 tcataaaacg acaagcatgc taaagcgaaa gaaacagcga aaacaattgt tggtggtggt
    11521 agcggctatg atcattattt attttctacc agttattaca caagtatttc atcaatattt
    11581 tggttaagaa tacgagagtt tgtgacaaaa gtcatgtcac aaactttttt ctttgtcatt
    11641 ttttcgtaag aaaaagcaaa ataaaagtat atacattgat taaaagtata tacttggata
    11701 taatgtactt actatagaat gagaatggta tagagggtgg cgaaatatga aaaaaatgaa
    11761 aaagtataaa attagaaaat ctggcaatag cgatgtaaca actatcccac cagaagtaaa
    11821 agaattcatg ggtgtgcaga caggtgacgc aatcagttat gtttttcaat ctgatggttc
    11881 tgtacgaatg atcaaagcac aagaagaacc tgatattgat tcattagttg attcgatcat
    11941 gaatcaatat gaagacgcgt taaaagattt ggttgattta tgaggtatct ttcgactaaa
    12001 gaaattatca aaatcaacgc gaaagttatt ttaccgtcac tcgccaggcg aaatgatcgg
    12061 tgtaaaagat gcaaatgcat tagatatggc cgtcaaacag ccttcacaag cagtattcaa
    12121 tcaagaactc tatcctgaaa tctatgaaaa agcggcgatt ttagcaatta atttagctaa
    12181 aaaacatcct tttcataatg gcaataaacg aactgctctt gttgccatgt tgttgtttct
    12241 tcaattgaat aattgtccag ttgctttttc tcgtcaagaa gctgttgatt ttatcataat
    12301 gattaccact agttcattgg atttcgattc actaaagtcc aagataacca gttatttacg
    12361 acaaaccgca ataaaatgaa cgaattaaaa actttatttc tcaattagat cagcatgtca
    12421 ttaaggaata tgctgatttt tttatgttta atcatacaaa atagtttttt cataaaaata
    12481 tggaaaatat ggattaattt tgaaataatt gatttaaaag gaatacaaaa gagtgaatag
    12541 taggaaatag tggtttagaa ctagtgggct ga
//
LOCUS       DACDIE010000069        12307 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.069, whole genome shotgun sequence.
ACCESSION   DACDIE010000069 DACDIE010000000
VERSION     DACDIE010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12307
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.069"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(329..1177)
                     /locus_tag="IV250_002284"
     CDS             complement(329..1177)
                     /locus_tag="IV250_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP8769532.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1341..2243
                     /gene="gnd"
                     /locus_tag="IV250_002285"
     CDS             1341..2243
                     /gene="gnd"
                     /locus_tag="IV250_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HAP8769533.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            2245..3246
                     /locus_tag="IV250_002286"
     CDS             2245..3246
                     /locus_tag="IV250_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP8769534.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3243..3527
                     /locus_tag="IV250_002287"
     CDS             3243..3527
                     /locus_tag="IV250_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP8769535.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            3560..4357
                     /locus_tag="IV250_002288"
     CDS             3560..4357
                     /locus_tag="IV250_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP8769536.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            4371..5204
                     /locus_tag="IV250_002289"
     CDS             4371..5204
                     /locus_tag="IV250_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8769537.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            5222..6079
                     /locus_tag="IV250_002290"
     CDS             5222..6079
                     /locus_tag="IV250_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8769538.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            6101..6610
                     /locus_tag="IV250_002291"
     CDS             6101..6610
                     /locus_tag="IV250_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8769539.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            6647..7048
                     /locus_tag="IV250_002292"
     CDS             6647..7048
                     /locus_tag="IV250_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769540.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            7160..7513
                     /locus_tag="IV250_002293"
     CDS             7160..7513
                     /locus_tag="IV250_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769541.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            7547..7990
                     /locus_tag="IV250_002294"
     CDS             7547..7990
                     /locus_tag="IV250_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP8769542.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            8007..9092
                     /gene="uxuA"
                     /locus_tag="IV250_002295"
     CDS             8007..9092
                     /gene="uxuA"
                     /locus_tag="IV250_002295"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP8769543.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            9085..9798
                     /locus_tag="IV250_002296"
     CDS             9085..9798
                     /locus_tag="IV250_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP8769544.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            10550..10750
                     /locus_tag="IV250_002297"
     CDS             10550..10750
                     /locus_tag="IV250_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP8769545.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
     gene            complement(10854..12155)
                     /locus_tag="IV250_002298"
     CDS             complement(10854..12155)
                     /locus_tag="IV250_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP8769546.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT         4062 a         1977 c         2633 g         3635 t
ORIGIN      
        1 ataacctaaa atgaggccaa aagccagtgt tatcaaggaa atacttaatt caccaaaatt
       61 ttttttaatt ttttagaaaa taagaaacaa aaataataaa aaaaagaaaa gataaatttt
      121 ttactactgt atactaactt tgttatcgca tctaacaagt aactcgaata ataccaatac
      181 aaccttctcc aaagatgcga tgacatacgt tctaacccac cgactattga tgttctgtac
      241 cacccttgca gaaccaaaaa agccactacc aaatatttgg tagtggcttt ttttgtttta
      301 tggatttttc gctttttctg ttatggtgct attctttgat ttcagaaata gtatgtcgaa
      361 ctttagcgat agaacgacgt gattcttttt cgttgtaaaa catcaaaatc acatatagag
      421 agtccaaaat gcttagttga gaaatacggg aagctaaagc ttcagatcgg aattctgttt
      481 cttcagaaat agaaataaaa acgacatcgc caagtttagc aagggaagaa ttagcttggc
      541 tagtaataac gattgttttt gctccagttt ttcttacggt ttcagcaata tggattgttt
      601 cttttgattg ccctgtgtga gaaatcaaaa cagcacagtc tttttccgtc aataaagaag
      661 cttccatcaa ttgaatatgg taatccgtgc tgtgagaagt tgcgataggg gaacgcaaaa
      721 atttatgata agcgtcagtt gctacaatct ctgaaccgcc aacaccgaaa aaatagacac
      781 gtttagattc attgataata gtagccgctt tttttagatc cttttgttgt aacaactttt
      841 ttgtgtcatt taaagttgaa atactagact cgaacacctt ttgcgccata gtgagctcag
      901 tatctgtttt ctgaatattt tcgtggatag aaatagcaga tagatcattt tcttggatca
      961 acatggccat tttaaaatct ttaaatccat tataaccaag tttttttgta aattgaaaaa
     1021 aagtagagtc agcaatgccc agttcacttg ctaaattgct aataggacta tgacttactt
     1081 tgcttggatt ttctaaaata taatcggcga tagcctgttc ttttgggctg aagtcgttgt
     1141 acattgtttt gatttgtagt cttatggaga cgctcatgag aattcctcat tttcataata
     1201 agattatcat tttttccgtt ctcattaatg ttaaagatac aaggagagta ggaaaaaatc
     1261 aaacaaaaaa tatttttgta aaatctattt acaaaaaaat ttttttgtat aatgagggta
     1321 taagaaatgg aggcgctatc atggaaattg gtgtaattgg tttgggaaaa atgggattga
     1381 atttagcttt aaacttaaaa gagcaccatc acaaagtaca aggactcgac atttcaagta
     1441 cagctgttag tcaggcaaga gaaaatgaca ttcctgctta tcaagagatc tctgaatttc
     1501 tagctacttt tgagcagaaa aaaatcatct ggttaatgct tcctgctgga gagataaccg
     1561 aaaacatgtt aagaaaactt tttccaaaac tcggaccagg agacattatt attgaaggcg
     1621 gaaactctta ttataaagat tcaatccgtc gagcagaaga gtttcagaaa aatgaaatcg
     1681 gttacttgga ttgtgggact tctggaggaa tcgaaggagc acgacatcaa gcctgcttaa
     1741 tgattggagg agataaagaa acttttctat ccgtggaaca attattcaaa gatgtagcaa
     1801 ttgaaagagg atatctgtat gctggtgcat ccggaagcgg acacttcttg aaaatgatcc
     1861 ataatggaat cgaatatgga atgatgcaag caatcggtga aggattccaa cttgtacaaa
     1921 aaagtgagta tgcttttgac ttggaacaag tcgcacgtgt ttggaatcat ggttcggtga
     1981 ttcgcggctg gctgatggaa atcatggaag atcagttaaa cgaacatccc aatttaagta
     2041 attatcgggg aatcgtttct gcatcaggtg aagcaaaatg gacggttgaa acagcgctag
     2101 aaatggctgt tccagttcca gtaattgcaa ctagtttgtt catgaggaat ctttcacagg
     2161 aagaagatag tttttcagca aaagtagttt cagcactaag aaatgggttt ggcggacatg
     2221 aaatagttga aaagggtgag taaaatgcca gattatcata tagcaatcgt taattccagc
     2281 agtttcggaa gagtctttcc acaccacttg gaaagattaa agtcagttgg agacgttcat
     2341 ttctttactg ttgatcaaga gattccagga aaagaactag cggagttact taaaggattc
     2401 aatatcatta ttgcaagtgt tacgccattt tttcgacgag atttttttga aaacaaagat
     2461 gaactgctat tgatttcccg acatggtatt ggttacaaca atattgattt aaaagcagca
     2521 aaagaacacc agacaatcgt atctatcata cctgctttag ttgaacgaaa tgctgtagca
     2581 gaaaataata taactaattt gttgtccatg cttcgacgca cgacacaagc acaagcaaga
     2641 gtcaaggacg atcgatggga agatcgggcg gagttcgtcg gtaggacgct gtttaacaaa
     2701 acagttggtg tgattggtgc aggaaatacc ggaagcggag taatcgaaat tttgcgtaac
     2761 ggttttcgct gtgatgttct agcttatgat ccttacaaat cagatttata catcgaaagc
     2821 tttggtgcaa aaaaagtaga acttcaagaa ttactggaac aatcagatat catttgttta
     2881 tgtgcgaatt tgacagaaga gaactatcac atgatttctg ataaagaagt cgatcagatg
     2941 aaagaaaatg tttatctatc taatagtgca agaggcgctc tagttgatga agaagcgatg
     3001 attaatggtc tgaaaagcgg aaagatcgca ggatatgcca cagatgtttt agaagaagaa
     3061 ccgggacgtc aagatcatcc gtatttagcg tttgaacaag tagtgattac gccacatata
     3121 tcggcctaca caagggaatg tttagaagca atgggtgaaa aatgtgtcag agatgtcgaa
     3181 cagatcagtc agcaccttct tcctgaacgt tctgtccaga caaagagcaa ctatataaaa
     3241 ggatgatgaa gaaaatggtg aacggtttaa aagtgaaaac aggccctcaa ttttatttat
     3301 atgaagaagg tggtatttca aaagtttctg atctcttgaa atcatacggc gcgaaaagag
     3361 tactggtcac ccacggaacg gtatcatggg aaaaggcgct acctaaatta gtatttttga
     3421 atgacgaaac gattcaattt ttttatcatc gttattctgg agaatgtagt tatgctgaag
     3481 ccagacgaat agctacaatc atcaaaaaaa tgaaatcgat ttcgtagttg gcgttggcgg
     3541 aggaaaactg accgatctag tgggttatag tgccaatctt gcaagaatcg atttcggagt
     3601 catccctact ttagctagta attgtgctcc atggacacct cttgctgtca tgtataagga
     3661 gaatggacaa gcagaaggga aaacagaaca cttttacaga caagctgctt tttttataac
     3721 agatccccaa ttagtgattg attcccctgt tccatatttc attgccgggt tagcagatac
     3781 actagcaaaa tggtatgaat ctgagctcat cttagaacaa gattttttgc aagatgaatc
     3841 atttttgaag ctagcacaag atactgcgaa gatctgcaaa gaagagattt tgaatcattc
     3901 tgctaaagca atccaagata tggcacaaag aaaactgaca tcggagttca aacagctttc
     3961 agagattatc tttgctgttg ctggtttagt tggaggatta ggagataaat acgcaagaaa
     4021 tgtggcagca catgctatgc atgacgctat cagtaaatat attccggaaa gccatcgttt
     4081 tctacatgga gaaaaagtag cgtatgggat tttctatcaa ctggcactag aaggtcgctg
     4141 ggaaaaaata gacgagttat atcctttgta tcgggaatta gaactgccgg tttctctaga
     4201 acaaatggga ttatttccga aagataaaca gataatagat aaaatggctg aatttattga
     4261 ttcgaaagag aaagtgcatt tgattcctat agaggtaaca cagaaatcac tgatacagtc
     4321 aatgatggaa ctggaaggtt atatcagaaa gatctgaagg aggaggacac atggaaacat
     4381 taagtgtagt acaaagtttg ttgattgctt tatgggtagc cgcggttatg tcccgctggt
     4441 taggtggagg agccacgttg accttacgtt tttctccttt gatgactgga ttagtcgtcg
     4501 ggttgatcat gggggaagtt gcacaagcga tgattgttac agccgctttg caaatgattt
     4561 atatgggggt tttttctcca ggaggatcaa tgccagctga accatctatt gcagcagcta
     4621 tcgcagtacc agtcgcatta ttaggggatc tgtcgccaga agcagcgatt gccgttgcgg
     4681 tgccagtcgg attgctcgga agttacttgt atcagtttcg ttttttcatc aatacttttt
     4741 taggtaaata tacagacaaa gctgtagaag atttgaatac aaaagctatg gcccgatcaa
     4801 ttattcttta tccaacggtt gcctcatttt tattattcgt tccgcttgtt ttcattgctt
     4861 tatatttagg ggcgccattg atcgctgatg taatcaaagc tttagaggga acagtcgtta
     4921 tccacattct tgaagtagta ggtggagggt tagcggcaat cggtatcgcg acaactgtat
     4981 acgttatcgg tcgaaaagat tatctggtct ttttcttcct tgcttatttt atgagtgttg
     5041 ttttgaaatc tttagatatt acgatggtta cttatgccat ttttgggatt attatcgctt
     5101 tgatctttgt ccagtcgcaa aaagggaaac aagtacagcc tgccacaagc aataacaatt
     5161 cagcatcttt tgatgacgaa gatgactatg atgacggatt ttaatttagg aggaatggga
     5221 aatggctgaa ataatggata atcaaacagg tgctcctgaa gaaatcacga aaaaagatgt
     5281 aacaaaagcc tatctgagat ggcattttgc aaacgaaatt cctcactctt ttgaacgtta
     5341 cttggcgcca tcactactat acggaatgat gccaatcttg aaaaaacttt ataaagatga
     5401 agactcttta aaagcagcat ataaacggca gttactgttc ttcaacactc aattaagctg
     5461 gggtggcggt gtgattactg gcctaatgtc ttctatggaa cagcaaagag cagaagaaga
     5521 gtatgaagaa aaagagatct tgatgcagga tgacttgatg tataacacga aagcgggatt
     5581 gatgggggca ctagcaggaa tcggagatgc aatcgattca ggaaccgtcc aatatatttt
     5641 tatcgcaatc gcggtacctt gggcacaaca gggaagtcct ttaggcgcac tttttccttt
     5701 tatctgcttt gggttgtatc aggttttact aggggtattt ttcgcaagac aagctttcac
     5761 tatgggccgt aatgctacag gcctaaagca aaacactggt gttcagacag cgattgaaat
     5821 gctatcagtt ttagggttgt ttatgatggg ggtgcttgcg ggaaactatg taaaagtaga
     5881 atcttcattg aaatttgcga tatccggacg tgagtttgtt atccaggaaa ttcttgatca
     5941 aatcgtgcca gggatattac ctttagctgt cgtaatgggt gtgtacttgt tctatacgaa
     6001 aaaaggattg aaagtcacac aagcattatt atggctgact ggaatattaa ttgtgctagc
     6061 aggaatcggt attttataaa aagaagggaa cgtgaataag atgggtgtag tcaatttagc
     6121 gcgtgtggat gagcgtttga tccatgggca agtaatggta acgttatctc aaaaaagtgg
     6181 agtgaattcc atttttgttg tagacgaagt tgtagcaaaa gataaattta tgagagatct
     6241 ttacaaaaat gcaggaagcc gtacaggaca aaaaacaatt gtcattacac cagagaaagc
     6301 taaattctat tgggatgaat atcaatttaa agaatacaat tgtattttga tcgcaaaaac
     6361 agtccaaacg atttacgact tagtaaaaca cggaatcccc atggaagaat tgaatattgg
     6421 agggattgct caaaaaaatc ctgatacaga tcttttagtg acgaaatctg tttatttaaa
     6481 taaatcagat gctgaaaaat tgaaagaaat gaatgaacaa tatggcgtaa agaatattta
     6541 ttttcaagcc actccatctg cacctaaaac aggattaaaa gatgtattga acaagtttga
     6601 tttgtcataa acaaatgctt agaaaaaaga taaagagggc gaaaaaatga agaagaacga
     6661 gctgtttcgt gattgggaat ttcgttatcg ctatatttac cgtaagcgcc gtacaaaaaa
     6721 gagtaaacag cgttttcttt cagcattagt atcagatatt tacagtatga gaacagatgt
     6781 gacagtcatt gcttacgata cgctagccta ccgatcaaaa aatatttatg ttggagatat
     6841 cgaaaaagca gagaaagtta tctgtactta ttacgataca cccgttcatg cattaggaag
     6901 ttatttcatg tttgattgga aagaccaaag gaaaaaaaca atctactcta tattattgtc
     6961 cttcattttg ttgttcagtt taggttggtg gggaatgatg atatataaca aaaatcctca
     7021 tcacgtattc gatctgctat ccgtataaac aggtatcact gtactagcat ttgggagcta
     7081 ctttttctta ttgggaaaag cagctcgcgg atggcctagc agacaaactt ttattcgaaa
     7141 tacttcatcc atattaacga tgctagaaat gatccgtacg attgatgatc cgagtgtagc
     7201 gtatgcgttt gttgatgaag gctgttatgg agaaaagggc ttagacagcg tgagatcggg
     7261 tatgaaaaaa gaggctattc ttttttatct agactctgtt ggggcagata caccgctaca
     7321 gttttcggga aattattttt caaataaaga acagtggctg aaacaagttg ataagttgaa
     7381 agaaaaaaac gtcaattata tttttagtgc aagaaaaaag caagcgcagt tcttctattt
     7441 gaccaaaaca gatttacgag ggaaaacatt taattggcaa aatgccaatc agattattgc
     7501 tctttttcga taagtaaaaa gtgcaaaaca aggagtgaga agactaatgt tggaaatcat
     7561 gatcgttaca catggtaagt tgagcgaagg actgaaagat gcggcggaag taattgtcgg
     7621 gacatcgagc catattgaaa caatgtgctt agagtctggg gaggacatcc aaacattcgg
     7681 agagaaggtc aaagaaaaat taactatgat caacacagca gaacccatgc tagtatttgt
     7741 tgatctaatt agcgcaagtc cgtacaatca agcgctcttg gcagtcaatg aactgactga
     7801 agaaaagaaa gcagccactt atgtcatagg gggcgtgaat ctgccaatgt tgttagaagc
     7861 aatcaatcat cagatgcttc acacaccgat agaagaagcg gtggaacaaa ttgtcaaaca
     7921 aggaaaagat aatttaactg tttggcatgc ttcagcggaa gaaaaaaaat tagatgagga
     7981 cgatttttag aaggaaggct gaaaagatga agtggggatt tagatggtat ggtgaaaaag
     8041 gggattcgat cccattaaaa tatgttcgcc aaataccggg aataaatggg ataattggaa
     8101 ctttgctgac taaaatgcct ggagatgttt gggagatttc agaaattgag gagttgaaag
     8161 catctgttga gagccaagga ctggaactgc ttggaattga aagtgtagcg atccacgatg
     8221 caatcaaggc agggacagat gaacgagatt actatattga taactatata cgaacaatca
     8281 aaaacttgtc tcgatgtggg gttcgattga tttgttatag cttcaaacca atttttggat
     8341 gggcgaaaac tgatttgttt aatgaaaaca acgatggaag ctattctctt ttatacgatc
     8401 agaaaatcgt cgatcagatg gagccaggag aaatgtatca attgatacac agccagtcaa
     8461 agggattcaa attgccgggt tgggaagaag aacggttgaa aaaattcgct cagctggaaa
     8521 aactgtatga aggaatgacc gaagaagaat tatttgaaaa tctgacttat tttttagaaa
     8581 aagtgattcc agtgtgcgaa aaagcagatg taaaaatggc tatccatcca gatgatccgc
     8641 catgggaact ttttggtttt cctagagtga cgaaaaatct tggcgacttg aagaaaattc
     8701 taaatgcagt agattctcca tataatggac taacgctgtg cacaggatct ttaggggcaa
     8761 atccggaaaa cgatttagtt gaaatcatcc atgaggtagg ttcgcgcatc cattttgtcc
     8821 acttcagaaa cgtggagttt ttaggagaaa gaaagttcaa agaaacggca catccctcta
     8881 cagaaggctc ccttgatatg tatcatatta tgaaagcgtt ggtgcaagaa gggttcgacg
     8941 gtcctatacg ccctgatcat ggtagaaccg tgtggggtga agtggctatg cctggatatg
     9001 gcctgtatga tcgtgcaatg ggactcactt acatgcaagg tctatatgag gcgataagta
     9061 aggaaaaacc actgggagag cattatgaat aaaatcaagc tcttcatctt tgatatggat
     9121 ggccttttat tagaaactgg acgtttagct tatcgtgctt atgtaaaatc agcacaaaag
     9181 tatgattatg aaatgcgtaa ggaagtctat tatttactaa ctggccaaac agaaatggcg
     9241 ataagagaac aaatgggcat tttgtacgga gaagatgttc catatatcaa atggcgagaa
     9301 gcaatcaatc aatataaaga aaaaatcgtg aaagaagata agagagtata tacgaaaaaa
     9361 ggagcagaag aaattttatc ttttgcaaaa gaaagaggaa tccatacaat cgtggcttcg
     9421 tctaatacac gggtaaaagt agaaatgtat ttaaaaatgg aaaatctata tgccttgttt
     9481 gatcaaatca tttcagggga tgatgtaaaa aaaggaaagc cggaaccaga gatctttttg
     9541 aaagcttgtt caaaaatgaa tattcctcca agtgaagcac ttgtatttga agactcgatt
     9601 gcagggatcg aagcagcaag gcgagcgggc attttgtctt ttctcatcaa agatcatttg
     9661 atagggttgc ccgatcataa tgggaagcat aagttgaaag tagatgtaag tttatatgaa
     9721 tccaataaag cagactatga attttatgac ctgcatcaag caaaacgatt tttagaaagt
     9781 aaagggttgt atctctaata aagaggacta ttttctagtc ctctttattt attgtcctgc
     9841 acgactgagg gaacagatga aaaagaagta attttttatt ttgaagtatc taccttattt
     9901 gtagtgaaca atttagaaca aacacaaaac gcatctactg ttatctacca gtagatgcgt
     9961 ttaaagagaa gaacgagaat cgtccatatg agggtttcca ctccttatgc agattttttt
    10021 aattcttgca ctgcttgttc gatcgtaaca cctgtcgcta catgattggt attttctttg
    10081 tcaaaaacga caaatagatt caaatctgta tccaaggtta ctcgatattg tatatctttg
    10141 ttatagaatg tcatagccat tctcctttct ttaaatactt gctgaaaggc aacgaacgcg
    10201 ttgataacga aattccattt ataagtatac caccaattga aaggttttca tagttaagaa
    10261 attttaaagt caaaagttgc agataaaagc aaataaacaa gaaaacaaaa agtgaatcaa
    10321 tcgcggttat tcaacttttt cttcgattcg aagacaatag aagacaatga attttatatt
    10381 tcattgcatt atccttgtgt tattagaaaa agcatgatat attaaatgag taatctattt
    10441 tgaaacgtaa tttgagcgat atatctacat agataaaact ccttttgcct atcaaatatt
    10501 tattgcaacg aaaaaacgta cattgatacg tatcaggagg aatttattta tgaataacgg
    10561 tacagtaaaa tggttcaacg cagacaaagg ttttggcttt atcactggtg aagatggaaa
    10621 tgatgtattt gcacattttt cagctatcca aggtgacggc ttcaaaactt tagaagaagg
    10681 ccaagcagtg tcttacgatg ttgaagatgg tcaacgtggc cctcaagcaa caaacattgt
    10741 taaagcttaa taaaaattga cttttaaccg tcttttcgaa agaaaagggc tctttgtcaa
    10801 ataggactga tagagtgaga taagaacatc taaggtagaa ttccttttct actttagatg
    10861 ttttttgatt cataggtaat cagattattt agtaagaaaa ttctggtctt cattttccga
    10921 aagcttcgaa agccataagc gactcgtttc aatgctttga tgtgggtatg cagattttct
    10981 attttggcat ttgaataagg caattcgagg gctaaacgaa tgccggattc atagtttata
    11041 aaagcttttt ttatagaatg cagttctttt ggaacggaat tgggcatcgt tcttacaagt
    11101 ttaaagaaag tgtccgcctc tttgtcgtga aacgcttgaa gaatttcttg atagtaaaaa
    11161 taggcgattc gtaattcgtc agagatcgaa agcagacgat ccaccacttc actttcggtg
    11221 agatacgccc aaccaaaatt gcgccatttg gtccatttgt cataaacaag cttgtcctgt
    11281 cgtttgatca gaagccgaga caacgccttt aattgtcgat aatatttggc ttgtgttgga
    11341 tcgtttcgat caaagctttt catgattcgt acgcgaatat cctcaaattt acgaatgaga
    11401 tgtttgacaa tgtgaaaacg atccacaacg agttttgcct ttggaaaaca ttcctttatc
    11461 agagaggcat aagaaacatt catgtccgtc acgatgacct ttaccttcaa tcgtgcgcgt
    11521 cgttcaaatt gcataaaata agaaacgaga gcctttttct tccgtgaagg aagaatatcc
    11581 agtaattttc cagtatcgcc atccatacaa gaaaaggagt agggaccgac ttgatttttc
    11641 agtgaacgaa attcatccat taataaacaa cgcggtaacc aatttctttt tacttttgtc
    11701 atggggatca gagaagcgag tgtccgagaa acggtcgacc aagaaacgcc gcaatgctta
    11761 gcaattgtcg tcattgcttg tttttcagtt agctcttcta aaattttata gcgcacttgt
    11821 ttagcgataa agcagtttct atcgacaatg taggtattcg ccgtaaaata ggtgtggcag
    11881 ccacgacagt ggtagcgttg tttcgctaaa gcaagataag tcagttggtt cgcacattta
    11941 ttaagttgaa tgagcgattt tttctttccg ttcttaacga tcatgccttc atgattcatc
    12001 tgtttacaca aaggacagcg acgaatttct ttacttaacg tcgcagaaac aacaagactc
    12061 ttgatattct taatgctctt cgattccaca tttgtgatga aaatgttttt atctttgatt
    12121 ccaagtagta gtttgatatg atgaagatca ggcatacaga acgctccttt gattgtgttg
    12181 tggtgattca attctaacgg agcttctgta tgttttgttt tttcatgctc aaaagggacc
    12241 gatagaatga ttttctatca gtcctagaaa ttatagagcc aagaaaagac ggtttttttg
    12301 tacgaaa
//
LOCUS       DACDIE010000070        12284 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.070, whole genome shotgun sequence.
ACCESSION   DACDIE010000070 DACDIE010000000
VERSION     DACDIE010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12284
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.070"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(26..328)
                     /locus_tag="IV250_002299"
     CDS             complement(26..328)
                     /locus_tag="IV250_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8769547.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(353..2446)
                     /locus_tag="IV250_002300"
     CDS             complement(353..2446)
                     /locus_tag="IV250_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP8769548.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(2935..3699)
                     /locus_tag="IV250_002301"
     CDS             complement(2935..3699)
                     /locus_tag="IV250_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HAP8769549.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(3810..4679)
                     /gene="mdcB"
                     /locus_tag="IV250_002302"
     CDS             complement(3810..4679)
                     /gene="mdcB"
                     /locus_tag="IV250_002302"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HAP8769550.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(4704..5780)
                     /locus_tag="IV250_002303"
     CDS             complement(4704..5780)
                     /locus_tag="IV250_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAP8769551.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(5804..6397)
                     /locus_tag="IV250_002304"
     CDS             complement(5804..6397)
                     /locus_tag="IV250_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HAP8769552.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(6401..8077)
                     /gene="mdcD"
                     /locus_tag="IV250_002305"
     CDS             complement(6401..8077)
                     /gene="mdcD"
                     /locus_tag="IV250_002305"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HAP8769553.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(8067..8366)
                     /locus_tag="IV250_002306"
     CDS             complement(8067..8366)
                     /locus_tag="IV250_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HAP8769554.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(8468..10114)
                     /gene="mdcA"
                     /locus_tag="IV250_002307"
     CDS             complement(8468..10114)
                     /gene="mdcA"
                     /locus_tag="IV250_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HAP8769555.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(10121..11032)
                     /locus_tag="IV250_002308"
     CDS             complement(10121..11032)
                     /locus_tag="IV250_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP8769556.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            11152..12009
                     /locus_tag="IV250_002309"
     CDS             11152..12009
                     /locus_tag="IV250_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8769557.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
BASE COUNT         3460 a         2625 c         2153 g         4046 t
ORIGIN      
        1 tttttaccct cttttctttt tgtatttaga taactccctt atcttgcaga actggggtga
       61 ttttttcttc aatttcattt tttgaaacaa tatttttcaa atagatcatc gccgttttag
      121 gatcaactgc gaacttttcg aactgcgaac gaaaattttc tgctgtgatg atcacatctg
      181 gtttcatgga aactgcggaa gaaatatcac aatggtccag tttggcttct actcctaatt
      241 tgttcaaaac atcctcagca gacatctgtg ctgcaaaact acttcctaaa cctgcaccgc
      301 atacgaataa aatgttcaat ttgttcatct ttgttctccc ccgtatcatt tattatttct
      361 caactactga aaaaagcagt tgtttgaaat cttcgatatt tgtgcattca atcaatcgat
      421 ccaacttctc ttcgttgttg atcaattcaa ttagttcttt catgatgttc agatgcgagt
      481 aggagtctac tgctgccaaa caaaaaataa tctttactgg atccatttca ggattaccga
      541 aatcgattgg ttgctcaatc gtagctacgc tgactcctaa tttattgacc ccatcttctg
      601 gtcgggcatg ggctagtgcc atgtgtttgc caatcacgat atacggtccg taatcattca
      661 ctgcacgaat catcgcttcg acatagcgct cttcgacaat gttttcttta accaatggtc
      721 tagcagctag ttcaatcgat tcctcccaag tagtggcttt ttctttgatc atgatattgc
      781 tgtccgtcaa aatatctttt aacataggct ggatctccct tttgttgatt tctaaattgt
      841 ttgtttcgaa taatttttct aacttgccat agatgattcg atctactcgg ccaccacttt
      901 ctttcatgac atctattatt tgagtgaata actccgtcga ttcatgactg ttaggctcta
      961 accgctgtag ttcttcattt cttttcaaaa agtcttctat aaccaagata ttcgtctcag
     1021 ttaaaatcgg atcgacaact aaaagcggct tatttcgata attcaattga aaggtgctga
     1081 aaaccagatt gacatccaat ttatctacta attcgatttc ttttgaactt aaaactgcag
     1141 taatctcgat ctgaggaaac ttttctttca gattctctgc taacagattt ccagtagcca
     1201 tcccatggtt acagacaacg actgatttat aaacataatt caagtctcgc ttgattacac
     1261 tggcgatcgt cgaaaagtgg atgacaagaa aagctatctc gtcctccagt atcttgccac
     1321 caattacttc ttcgatcgta ggaacaaaat gagtgactgc gcggtagatt gggaaataat
     1381 ttgttcggat gttttctttc aatggattca tgacttggat atgattcttg attcttacaa
     1441 tcagtgcagc catgtgctta tagagatttt cgaataaagc ttcttccttt agatggaaag
     1501 gtacgtgtgt ctcgttctct acaaattgga tcaattggat cgttagtaat tgagcttgta
     1561 cccactcgat tgaattggag atatctctag cattgaacgt ctcaatagtg aaacgaagat
     1621 aatttctttc tacttgcggg acattgtcca attgaaattc ctcgatcatg tggtcaataa
     1681 atgcagaaaa gctactgtct tttatttctg tatttttcca attaatggca gagataaatt
     1741 catgtttcct gattcgctgg atccagatca atgtaaaaag caggatttgt tttttataaa
     1801 tatcatcttc ttgtttcaac aacttattgg aatagatctc ctgtattttt ttgatttcca
     1861 ccaaaggaaa ataatggaga aagatttttt gagccggtgt taattgtgca tcttgattca
     1921 taaagaagtc ggtcattccc agattctgat tgataaaatc atagatcatc gtacggatag
     1981 atcgttctga acctttataa accagtcctt gctttccata actgacgatc tctatcccat
     2041 aactgaccag tgttgctcgt aaccgtctca tatcttcatc taatgtactt ttagagattt
     2101 gaaattcttc ttcttttttg tttagatgga tcgggattcg gctgtaagca acggctaaaa
     2161 ctagatcaaa aatacgttcg cgccgattca aaaagccgtc tttcacatct tttatttcag
     2221 tttctaatcg agcttgcttt tccttttcca gcttcaacaa aaaacctttg ttgcgaattg
     2281 tacggatctc cggaagcttt ttactgctca accagatatt cgcttcaaga acttcctgac
     2341 ggatcgtccg ctgactgaca ttgaactcac ttgctagatg ttttgctttt actggatatt
     2401 gggctgtcat gattctgtga agtatttcaa ttgttcttgt tttcatagaa atgatctcct
     2461 tctgatttca agtatatggt tccgcttaca taataaaaag tagatatttc tgccacttga
     2521 agtggaaaga aagtaagcaa agcaaacaaa aaataagcca tatgcttttt catctcctca
     2581 acgaaaaaga catacgccta ttattacaaa aattttgccc atctatacaa aaaagaggtt
     2641 gtgaaaaagc tgtcctgcat caagtaataa gaacaactaa actaaaatag tgcttcctat
     2701 ttttcgtttg gttgttctta ttatcgcaga tgcaagcttt taaacaccat ttagtggaga
     2761 ggttgtgaca gagatgttca gccttgagcg ataagaagcg attttgaaaa atcgcttccc
     2821 atattttaca aaatcgtgcc ttattgccga taggctatct ctggcacacc gtttattcag
     2881 aaaaaggagc tgcgacaagg cttttgtcac aactccgttc ataaatattt cctgtcaatc
     2941 cagcataatc ggctgaataa aatcggcttg ccatcttttt tctttctcag aaaaatcaaa
     3001 ataccatatc tgagaaaaat actgtcgttg tgtcttactt cctgagatca atcgcccatc
     3061 ttttcgatcg actgaataat cacagcatct gaaatcaatt cggatggcaa aactattttc
     3121 gtggatctgt cgataatttc ctacttcctg aatagttatt tttttagtat gatttcttac
     3181 accgtctgct ttgttttttt gcagaacttt ctgatgttcg gcaaacagct tgtctgtata
     3241 acagcttctg gctttttcag gagtttcttc ttcccaagcg gattggatct tcatgaatat
     3301 cttttggatt tccagaaact gtgctttctt ttctttagcc gttcctgaca tgcgggacaa
     3361 tatctccgct ttcagccttt tcttttgtct tttattcttg acgatagaaa aagcagaaac
     3421 ccctcctact gacaaaacag ctaatgcaag aaatcccgtt tgctcataac tagaaggacc
     3481 tgtacctgga ccataatacc cataagaatc tctgccgtaa gaacgactgc caaaataact
     3541 gcccccatat gaacgaccac caaatgaacc tctgcccgaa cttcgaccgc tagatgtacc
     3601 tccaccatga ccacccgcga cagcgaatcc ttgggtcggc agagcaaaca aacataggcc
     3661 cactaacaaa atgattgcta aataattgat ttttttcatt gtcttcctcg ttatctttta
     3721 ttacaaaaaa ctatttttat gatagccttg ctaaaaggaa gagtcaataa ccagatgcat
     3781 aaactgtttt tttagcaatt ttgtcttatt tagtcattga gtaacatcgg acaaatgtat
     3841 gtgtcggcag tccataattc ctctatcttc agcatgaaca agcttgcaga caagagatct
     3901 gcgcttcctc caggagagat ttggttttcc tgacaaaagt gcaacagtgc tgtaaatgga
     3961 cgatttggta aagcactttg actagcagca tgagcaagtt tttgcatttc cactaacgtt
     4021 ttctgatcac tgcgatgcaa tacgcatgtg tcatctagcg aactcatcaa aactaacaac
     4081 atatgcattt gtttctgtac atatgaatca ttatctagct ccttagctgc ttgaattgca
     4141 agcctgatat gcggataccc taatatcgct tcttcatatg caccatttac gccatatttt
     4201 tgcttcaccc tatgaccatg agtggttttt ttatgacgat attttgtatc aggaaaagca
     4261 gcaagcttgc tgacatcatc aagtattttc gataaagaaa aaggctgttc catactgatt
     4321 ttttgtgcaa tgacactgat caatagcccc attgcccaaa tcgctccttt atgggtattg
     4381 atgccatttg tagcaatcag cattgccttt tccgcttttc gcccaatctc agcaattctt
     4441 tctcttaact cctgatcaat agcatgatgc catgctattt gagccatttc ttcaaaaaaa
     4501 ggagttagcg tcttagcaga cttaagaaat aaagcatagt ccatatcgga atgtgcaccc
     4561 gcatccagtg catccactaa tccaggtttt ggacttaatg ttacttctgc gatcaatgct
     4621 tcctctacca acgcagcgag ttgctgagtc actgcttttt ttcgttcgtc atttatcatg
     4681 acactcactc cttcttccta ctattacttg ctgaaacctt ttttgatagc tacccaaatg
     4741 atatgaagta accagcttaa agcaaatgca caacttgccg tcattagata agctacaaga
     4801 atcatgactc ctgaaggtaa ctgcagccaa ctatctttaa acaataataa cagtacttgg
     4861 aaaacaaata cattggctaa atacgtacga taagcataaa cagacaacca tttgaacaat
     4921 ggcaattttg gcgaatcgtt tacaatcatt ttggaagcgc ctatgaagac agcaaagatg
     4981 atgactaaac tgtataaact ttggatcgta ttcaagtaag gtgcattcgc aaagctcaag
     5041 tctcctggat gtgcgagcaa tgaacggaca gaaaagaacg cgataactaa ggcaacaggt
     5101 aagaaaagat aacgtactct gtccaaatat tgatagactt tttcgtgata aacaaatgcg
     5161 gctaccccat aaatcatgaa actagcaaca aaccgatcta ataagtacca gctgtcatga
     5221 taaggcccag tcaatacttg tgtttgatag aacagatacc atgcgacata taaaatcgtt
     5281 gaaccaatca atactggcca tacatatttt ttattagcaa atactttgta accgagccaa
     5341 acaaaaaacg gcatgaacaa ttggatctgc agcatcataa cggtatacca taaatgaggc
     5401 gctccatcac ctgtgacata tttcagtaaa aagcttgcag tatccgtata cggagtttgt
     5461 tgttggactg ctggaaatag ccaaagataa gccgtcgtcc atagaagata cggaacgact
     5521 agttcaaaaa acttttcttt caaaaattcc caataagaca aatgctgact tgtttttacc
     5581 atattataaa caattgcaaa aataaacatt ggtgctgtgt atttaacgat gacgtaaaag
     5641 gtaccaagaa aagctgtatt tgttgcgctg tggctgtttg ccaaagcaaa gctcaatatc
     5701 gtttgcatca taacagccgt tacagctgat actttctgga agtcacttac gttgattttg
     5761 atttttttgt tttcaaacat ctctttctct ctctttttct ttattaccaa agttctttgg
     5821 ttaatttagg tccttcgact gttttcaata gaattttttt ctctggctct ctcaaatact
     5881 cttttagtga aaaaccgcct tgccgagtca ctacttgggt atcgaattgc tcaaaaaaag
     5941 caggatgcga ttgaattacc tgaattggca attcaatcgg tgaatttgcg tacagcagca
     6001 aatcaaaatc actggtttct tttactgccg gaatcccagt cgataattca aatcctaagc
     6061 tgccgccgat tccccattta catcctgata atatgtttct agcagcatga aattgctgat
     6121 aaacaggata atccatgatt ttctcttgtc tatacgattc ttgttcgatg atgtcccacg
     6181 gatgaatgac ttgtttgacg gcatgttttg agatttctgt tgaccatctc tgattgcggg
     6241 caggtccacg taaaccaatc ggtagtattt ctgcgtgatc cgtcgacgca cgacgaactg
     6301 ttccgaacat cttttcagtt agaacgaccc attcaggcaa ggtgaaccct tctatttcat
     6361 gtttatcaaa aaggacaata tcatgtgctt gaatcatttt ttatgcatcc cattcttcgt
     6421 ccattttggc acggacttta tttgtttcga cacgtccgaa tgttttggct gtttcgtttg
     6481 tgtatcgatt tgtcagcatc gtgtcatttg tattgcggac atcttgaatg gcttcattga
     6541 ttgcttcgat gactttgctg acattttgtt ctgaagcagc ttggtcggtg atatcttcta
     6601 ggaaatgata gatcgctcct agttttgtat aattattgat atcgtaagcg atagctggaa
     6661 ctttttcagc agctgcttcg atttcagcaa ccgaacgttt tgtcacacgt gcagcacttt
     6721 ctttagacat cgcttggacg gtaatttctt tattgtttaa agcaatcaag cggcttgatt
     6781 gtaatccatg tgctaaaaat gcacctgaaa tagcatttcc tgcaatcaat ccaataactg
     6841 gatgtccagc ttgacggagt tttgcataag cagcagcact attagccaaa gccacatgga
     6901 taccgatcaa ttcttcgtta tagccgtatg cttggcttgg tacatcgatc acaacgatga
     6961 ttggccgttt cacttttttg tctttatctt cttcgtaaac gtgatccaaa atttgtccca
     7021 tgcggaaacc ttcaactaaa ccaacttcac cgtttcgtac acggtacatc gggtttttag
     7081 gatcaggtac gattgatgtc acgatggctt cttctatgaa tttacttgat ttaccataat
     7141 acacagtact aatagaagaa gttggatttt tcatgccagt tagtgccgca aaccaatcgt
     7201 aaccaacact tgctgcagct ggttcatttc cttctgctac ttctggtaag tcaattgatt
     7261 ttgcttgatg gttagcaatt gcttcattgt aatcgtcaat ggccattggt tttgaaagat
     7321 ctaaagaatc aagtaatgat aaataaaatg ctgagctttc actgcgatgc gtgtcttttc
     7381 gttcgtccat tgctgcgatg acagcagctt tgattgcttc tacactatca gtcactaatt
     7441 catcaatgat ttttgattct acccgttgac gagatcctaa tgtattccaa atcaagtctt
     7501 tgtcactaga atcaaattct cgaacgcctg cttcttgttc aataacttct ggtccattca
     7561 aaccgactct cgcttttttt gttgcgatta aataactcat taatgctgaa gtaatcgaca
     7621 taccgccaaa tgatcctaca cgtcctggaa tcaaaccgat gactggtaca tattgtttta
     7681 gagcaatgat tacatttccg atttctgaaa tagataacag tccgtagttt gcttcttgca
     7741 aacgaacacc acctgtatct agtacaatga ctggaaagat ctcatttcct tttttatttt
     7801 cttctaacac atgttcaagt gcagcaacga tttttgcacc ggaaacttca ccgataccgc
     7861 caccttggaa ttttccttcc atagcgatta ctactgcttg tttgccattg atcgtgccac
     7921 ggctgacaac gatcccgtca tcactttcag ggacgattcc ttgtgctggt aaacttggcg
     7981 cgatgatttg atcgaatgga tcaatcaatt cacgtccatt gtcaagtaga gcgttcactc
     8041 gttctctggc gtttaattct acaaaactat ttttcatctg aaagttcctc cattgcttgc
     8101 gtcaatcgta ggtacacaac tcctggtgta gcaccgaagt cattgatcac gatatctgct
     8161 gaaattggat aacgatcaaa aaatctcgtt aatacattgt tccatacttc gccgaagcca
     8221 tcgcttcctg tacaaatttt tacagtagat tctgtttttt ctgttggaaa catcaagatt
     8281 tccaaatctc ctgaaccgac cactcctaca tggattttct tattgatcgg ctgtttcgtt
     8341 ggatatttat aagctaattg ttccatggat ctctcccctt tttgtaaaac agagcataca
     8401 cgttctgctt attcgtcttt cttcttccat ctgggtaaat ggcgacaaaa ggtttttctc
     8461 gccaatttta tgaccaagtt ctgaatttca ttggtggttc atacaggcca tctgaccatt
     8521 cgatcaaatc atcgatggtt tgtgcagcta ataatgaacg tttcgcttca ctacgtttga
     8581 tgcctagatc ttctggtaat ttcacgatgc cggctgcacg caagctatct agtgattctt
     8641 tcgtgctagt cattccaaga ggtgtaacac cgccaattgc tgcaattgct gcttgtcgtt
     8701 cttcttttgt ttttgctttg tacaaataag caattccttc ttctgtcacg atatgtgtag
     8761 tatcttcgct ataaatcatc actggtgcat tggctaagcc tgcttgtttt tgaacagcta
     8821 gtgcatctaa ttcttcgaca aagactggtt ttttatttga accatatgtt tcaaccattt
     8881 gaacaactaa tttttggcct ttccctaatg gatcatcatt gtcgcgaagt gattgccatg
     8941 ctggtgtaga atgacgacga ccgccaggat tatgtcccat gttaggcgca ccgccaaaac
     9001 cagataaacg tccttttgtt acggtagaag agtttccttc gtaatctaat tgcaaggttg
     9061 aaccgataaa catatcgatg gcatattgtc ctgcagcttg tgataatgtt cggttactgc
     9121 gcatcgtgcc gtctttaccg acaaaaaaga catctgggcg agcagcaata tatttttcca
     9181 taccaacttc gccaccaaaa ctatggatgc tttcaaccca acctgattcg atggctggaa
     9241 tcatcgttgg atgtgggttc aatacccagt tcttcgcgat tttccctttt aaccctagtg
     9301 attcaccata agttggcagt aacaattcga tagctgctgt attgaatcca ataccgtgat
     9361 tcaaagattg tacgccgtgt tctgcgtaga ttcctttgat tgccatcatt gccattagaa
     9421 tttgcaattc tgtgatgttt tgaggatctc gtgtgaataa agcttctaat tgataaggtt
     9481 tatctgcttt cacgacaacg tctacccagt caccagggat atctacacgt ggtaaagaat
     9541 cgacaacttc gttaacttga acgatcacga taccatcttt gaaagctgca gcttcgatga
     9601 ttgttggtgt ttcttccgta ttgaaacctg tgtacaagtt gccgtcttta tctgctttat
     9661 ctgcagctac taaaacaaca tttggaatca aatcgatgaa taagcgtcca taaagttcca
     9721 aataagtatg gatatcacct aatttcattg tgccgtcttc aatcatttgg gcgacacgca
     9781 agctttgtgc accagcataa gagaaatcga tcttgcttgc gattcctttt tcaaaaatat
     9841 tcaaatgttc tggtcgtgag atactagaca tgatcaaatg cagatcattc actttttctg
     9901 catctacttt ttcaagactt tctgaaagaa aacttgcttg tttttgattg tcgccttcta
     9961 atacaacttt gtcgccggga gcaatcagtt tttccattac ttctacgatt ttttcagttt
    10021 ctacatactt gccattgagc aagttgctga ttttttccaa tcgctgcgct tttttttcgc
    10081 gtttagaagc ccagttgcgg tttggttttt ccataattag tcatctccat ttttgatata
    10141 gaaacgtggc gtcatcaata ccatgttggt ctagattgat cgtacggatc cctttatcta
    10201 caccaaattt tgtatgaatc agctttgtca aagtattgcc ggggggaaat tcaatcgtca
    10261 gatccattcc taattctttt gccaagtcca tcatttggtt ccactgcaca gggaaagcta
    10321 ggttttccaa caaatcttta cgaatctctt ctgcgtcctg agtggctctt ccatccgtat
    10381 tttttatata caaacatcta gcatcagata gatggacaga ctgcatatac ggcttgaatt
    10441 tcttgaccgt atcattcatc aagagacagt gagatggcac agggactttc agcagcttcg
    10501 ctgtttgcgc ttggtgccgt ttggcttctg ccaacgtaac ggataaacct tcgatactcc
    10561 cagataacgt atgttgcatc ggacaatttt catttgacag ataaactgga tggtcaggat
    10621 caaatgtttt tgctacaatt tcctcagctt cgcttctcgt cacacctact actacgccca
    10681 ttgcataacc gcttggataa gcttctttca ttaagactgc gcggtgatag actagttccg
    10741 ctgcattctc aaatctcaaa gatcgacaag caacagctgc acaaaaagca ccaatcgaat
    10801 gtcctgctgc aatatcaggc tgaataccgt ttttttccat ttcatctaaa taaaatactg
    10861 cttttgtcag taatgctagc tggataaaaa ccgtatcttg atagttttct tctttatcaa
    10921 ctaaccgata ccccgtcaca ttttctaaac gttccatgta catttcaggg atatccgcta
    10981 acatccctaa cttctgactg ccttgtccag gaaataagaa acctgtactc atgagcctcg
    11041 cctcctcagc acttactttt tccactgcta tcttagtcaa tttctgctat aataaaaaat
    11101 gccaaaaaaa gaaaaaacca taacaaaaag gtatggatgg gatggataat tatgaaattg
    11161 cttcatctgc agtattttaa aaaagtattc gaaacaaaaa atgttaccca agcagccaaa
    11221 gaattattta tttctcaacc tgccttgagc cgtgcgatca aacatttgga aaacgaatta
    11281 ggtgtccctt tgttttacca tagcggcaga aacattgaga cgaccgttta tgcagaagaa
    11341 ttttatccat atgcagtcaa gacattagac acattagaag aaggaatcaa attattgtct
    11401 tcggtcaatg agaagatcgt tacttccgtc atattgtatt tagaggtagc ctctgtttct
    11461 attccgaact tggtccgaat ttttatggaa aagcatcctg atatccaatt aacgatcatg
    11521 caacatcatt taccagatga tattaccgat cctgtactgt acatcacttc ggagatgaag
    11581 cctggcatga ccaatattcc tatcatgaaa gaacctgttt atgtcgccat tcctaagaaa
    11641 catcctcttg ctaaaaaaga acatctgcaa ttagaagata tccaacgcac ccccatcttg
    11701 atgctgtcaa agagcaacgc cttccgtcat acactggatg cagcacttga agcgaaaagt
    11761 atcgaattga cgatcagttc gacgacagat gatcctgcga cattacgttc tattgtcaag
    11821 caaggattag ctgttagctt tttcccaaaa gtttcttggt cttacgataa aaatgatcct
    11881 ttcgtcttgc gggaaatcaa agatttgccc ttgacacgaa caatttattt atcttctagc
    11941 ttcacagaag atcatccttt gatcaaaatg attgcaaaaa cgatgcggga attttattta
    12001 ggaaaataaa aaaaaaagag ctgggacaaa attcttgtcg cagctctttt ttcgaacaaa
    12061 cggtgttcca gaagtaattt cttagaaaat aagccgaaat ttcccaaaaa tttgagaagc
    12121 aattttcgta aattccttct tatttcttga agctaaacat ttctgtcaca acctcttctc
    12181 tattgtccta gacattcatt gcgttgattt aaaacttcca tcaatcaaat ctgctagttc
    12241 atctagcttt tctattttac gtagatcaat agagatccaa tgtt
//
LOCUS       DACDIE010000071        12233 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.071, whole genome shotgun sequence.
ACCESSION   DACDIE010000071 DACDIE010000000
VERSION     DACDIE010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.071"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(178..735)
                     /gene="frr"
                     /locus_tag="IV250_002310"
     CDS             complement(178..735)
                     /gene="frr"
                     /locus_tag="IV250_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAP8769558.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(738..1460)
                     /locus_tag="IV250_002311"
     CDS             complement(738..1460)
                     /locus_tag="IV250_002311"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAP8769559.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1596..2477)
                     /locus_tag="IV250_002312"
     CDS             complement(1596..2477)
                     /locus_tag="IV250_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAP8769560.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2576..3358)
                     /gene="rpsB"
                     /locus_tag="IV250_002313"
     CDS             complement(2576..3358)
                     /gene="rpsB"
                     /locus_tag="IV250_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAP8769561.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTVTSIEEIVDVVEGDNSSAE"
     gene            3717..4196
                     /locus_tag="IV250_002314"
     CDS             3717..4196
                     /locus_tag="IV250_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAP8769562.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4413..5504
                     /gene="phnW"
                     /locus_tag="IV250_002315"
     CDS             4413..5504
                     /gene="phnW"
                     /locus_tag="IV250_002315"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HAP8769563.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5497..5625
                     /locus_tag="IV250_002316"
     CDS             5497..5625
                     /locus_tag="IV250_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAP8769564.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            5629..6174
                     /locus_tag="IV250_002317"
     CDS             5629..6174
                     /locus_tag="IV250_002317"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAP8769565.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6628..8319)
                     /locus_tag="IV250_002318"
     CDS             complement(6628..8319)
                     /locus_tag="IV250_002318"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAP8769566.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8739..9686)
                     /gene="arcC"
                     /locus_tag="IV250_002319"
     CDS             complement(8739..9686)
                     /gene="arcC"
                     /locus_tag="IV250_002319"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAP8769567.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9801..10820)
                     /gene="argF"
                     /locus_tag="IV250_002320"
     CDS             complement(9801..10820)
                     /gene="argF"
                     /locus_tag="IV250_002320"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAP8769568.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10911..12140)
                     /gene="arcA"
                     /locus_tag="IV250_002321"
     CDS             complement(10911..12140)
                     /gene="arcA"
                     /locus_tag="IV250_002321"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HAP8769569.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3353 a         2533 c         2206 g         4141 t
ORIGIN      
        1 ttaaaagatt cccactatct taagtattat agcaatatct aactgttttt cctatgaaat
       61 cattgttttt ttatttaaaa actgaataat tttttctttt tttaattttt aaataaaaga
      121 aatataaata aaaacagagg aagcggaatg atccgcccct ctgtttttat gattttatta
      181 aacttccaat aattcttttt ctttatcagc agcgatctta tctacttctt tgatgctgtt
      241 atctgtcaat gtttgtacgt ctttttctaa tccgcgcaaa tcatcttcgg taatatcacc
      301 atttttttgt tgttttttat attcatcgat cgcatcacga cggatgttac gaatcgcgat
      361 ttttgcattt tctgcttctt ttttcacatc tttagctaat tcttttctgc gttcttctgt
      421 cagttgcggg ataactaagc gaatgacatt tccatcattg gttggactga ttccgatatc
      481 acttgccata atagcttttt caacatcttg aagaatgctc ttgtcaaaag gtgtgatcat
      541 taaaacacgt gcttctggaa tttggattga tgccatttgg ttcaatggtg ttggcgcacc
      601 gtagtagttc acagtaatac ggtccaaaag gctagcgttc gcacggcctg cgcggatttg
      661 cccaagttca cgttgaagat tttgtgctgc ttttttcatt ttttcttttg cttctgtcat
      721 aattgcatct gccattttta tttccccctt acagttgttc cgatattttc gcctaagatt
      781 gcacggcgaa tatttccgtg ttcatttaaa ttgaagacaa gcagtggaat atcattatcc
      841 atgcttagtg aacttgcagt tgaatccatg acttgtaatc ctttggcaat aacttccaga
      901 tgagtcagtt cttcaaattt tactgcattt gcatctactt tcggatcagc agaataaaca
      961 ccgtctacat tgtttttagc catcaggata acatctgcgc cgatttctgc cgcgcgtaat
     1021 gctgctgttg tgtctgtcga gaagtacggg ttgccggtac cgcctgcaaa gataacgatc
     1081 cgtccttttt ctaaatgacg ttctgcttta cggcgaatat atggctctgc gatttgacgc
     1141 atttcaatcg aagtttgcac gcgtgtcggt acacccacgt tttccaacgt atcctgtaaa
     1201 gctaaggcat tcataactgt agctaacata cccatgtaat cagcttgagc acgttccatg
     1261 cccatttgtg ctccgatttg tccgcgccag atatttccgc cgcccacaac gattgccatt
     1321 tcaacgccca gctcgtgaac ttctttgatt tcttggatga tttctttgat tactggtggt
     1381 ttgattccaa atcctgcgtc cccagctaaa gcttctccac tcaattttaa gactacacgc
     1441 tgatatttcg gtgtgaccat gttcattcct ccaatatttt ctattgccat tttaccatac
     1501 ttagatgatt tcgacactct aagactggtt tttcttgttt tgcttaaaaa aagggagcgc
     1561 acaaaccgtg cgttcccgtc tgttattcaa cagaattatt ttttcacttg gctcataact
     1621 tcttctacaa agttatcttc gcgtttttcg ataccttcgc caacttcgaa acgtacgaat
     1681 gttttaacag ttgcgccttt tgatgcaacg tatttttcaa cagtcatatc aggatcttta
     1741 acgaatggtt ggtccaccaa agcgatttct gctttgaatt tcttcaagcg tccttctacc
     1801 attttttcaa cgatattcgc tggtttgcct tcgttcaatg cttgttctgt aagaacagtt
     1861 ttttcatgtt ctaattcagc ttctggaatt tgtgattcgt ttacataacg aggattgata
     1921 gctgctacgt gcatagctac gtctcttgca actgtttcgt cagttgttcc atctagaaca
     1981 gtcaatacgg caatacgtcc gcccatatgc aagtatccac caaatgcagc attgtcgtct
     2041 ttttctacaa cttcaaagcg acggaagctg attttttctc cgataacttg agttgcttcg
     2101 atcaagtcag attcaattgt acctttatct gttttgattt tcattgcagc ttccatgtct
     2161 gctggtttgt tttcagcaac aagttctgca atttctttta ctaaatcttg gaacatttcg
     2221 tttttagaaa caaagtctgt ttctgagttt acttcaacga ttgctgcagt gttacctttc
     2281 acagcaactg aagccaatcc ttcagctgca atgcggtcat ttttcttcgc agcctttgcc
     2341 attccttttt cacgtaaaag atcaactgct ttttccatgt cgccttctac ttcaactaat
     2401 gcttttttcg catccatcat tcctacgcca gtcatttcgc gtagttcttt taccatttta
     2461 gctgtaacgt ctgccatgtt taactcctcc tgtgtgtttt tctttttaaa aaaagctgct
     2521 tcaaaggaga gtgaggcttg tcgcccagcc tttgaaacag ctccatcatt caaaattatt
     2581 ctgctgaaga attgtcgcct tcaacaacat ctacgatttc ttcgattgat gttactgtgt
     2641 tttcttctgt gaaatcttct tcaactactt gatcttcacc ttggtttcct tcgatgaaag
     2701 catcagccat ttttgaagta atcaatttaa cggcacgaat tgcatcgtcg tttgaaggga
     2761 ttactacgtc gatttcatct ggatcgcagt ttgtatcaac catagctacg attgggatat
     2821 ttaatttgtg tgcttcttga acagcaatac gttctttacg tgggtcaacg atgtacatta
     2881 catctgggat tcttggcata tcagcgatac cgcctaagaa tttttcaaga cgttcacgtt
     2941 ctttgttcaa accagcaact tcttttttag gtagaacttc aaaagttcca tcttcttcca
     3001 ttgcattgat ttgtttcaaa cggctgatac gtttttggat agtatcccag tttgtcaatg
     3061 ttccgcctaa ccaacgatgg ttaacgaagt attgtcctgc acgagttgct tcttctttga
     3121 ttgcttcttg tgcttgtttt tttgtaccta cgaataatgc tacgccgcct tcttcagcaa
     3181 cgctcttcat gtaatcataa gctgcatcta ctaatttaac tgttttttgc aagtcaatga
     3241 tgtagattcc gtttctttct gtgaagatat atttcttcat ttttggattc cagcgacgag
     3301 tttggtgacc aaagtgtacg ccggcttcta gtaattgttt catagaaatt actgccatgt
     3361 ttgtttcctc cgatttggtt ttatttttcc tcttcttatc ttctaccttg ccggcaaact
     3421 ttttcaagca cctaccgaca atccaataag aatgtggatt tgctgatgca taaagcaccc
     3481 tcagctatta tacagacaaa agatccgtat cgcaagagca aatatacttt tctgctcaac
     3541 attcaatagt ttacctgaaa gataaaaaga attcaagaaa aacagtagat ttctctttgt
     3601 ttctttatta tcctttttcc ataaaataat tttaggtata aaagacgaat attattgggc
     3661 aatccttctt ttttttgtta tgatacatgt gaaatctttc gtaaaggaga aagaaaatgc
     3721 gcaaagctca aagacatttg ctgattaaac aagtcattga agaattcccg atccgtacgc
     3781 aagaagaact attaaacaaa cttgggcatt atggcgttac cgccacgcag gcaacgattt
     3841 ctcgagatat ccgtgatcta aaaatcgtga aggcacctga tgaaaatggc gtttcccgct
     3901 tcgtattgtt tcgcgaaaaa ggaactcctg agactaggac tgaggaagag gatcggctgg
     3961 ttcaaatgat tgaagacatt gtattgaaag tcgaacgagt ccactttttg acgatcgtca
     4021 ataccttgcc agacaatgct catttatttg catctgtttt agatgagatc aaacctccct
     4081 ctattgtcag tacaattgct ggattcgata cgatcatggt catctcggaa aatgaggagg
     4141 acgcaaaacg agtagaagaa tttcttcacc atccaactga agtatcactt ttttaatcag
     4201 agcacgtatt cctaatatgg aatgcgtgtt tttttatttt caatattttc cttattgaat
     4261 aaacctttac actctttaca aaaatacatc attttttatt caaatttttc aacaccttga
     4321 ttgaatcaca ataatcatca tttatattgg atgttaacaa gagcagcttc ttgctctttg
     4381 tgtatttcaa aaaagaaatg aaaggaaatc atatgtataa attattaact tctggaccat
     4441 tgacgacttc tgaaacagtg aaaaatgcta tgctgatcga tcattgtact tgggatcaag
     4501 aatacaaaga aattacacaa tggataagaa aaaaattatt ggatcttgct caagtatcgg
     4561 aaggaatata cacaacggtc ttaatgcaag gtagcagctc cttcggggta gaatcggtat
     4621 taagttctgt gatcaggcca gaagatactg tactgatttg ttcaaacggc gcatatagac
     4681 aacgaatgat caacatgtgc gaatgtctga aactgaacta taccgtttat gaggtatcgg
     4741 aacatcaaat cctccaagct catgtgatta cacagctgat tgaagagaat ccgtctatct
     4801 ccgcactagc aatggtccat agcgagacta cttccggtat tttgaataat ttggaagaga
     4861 ttgctgctgt cacacaagaa agagatcttt tgtttatagt ggacgcgatg tcaagctttg
     4921 gtgggatacc gatttgtgta gccgaactag ggattgattt tttgatttcc agctcaaaca
     4981 aatgtattca aggtgttcca ggcttttctt ttgtacttgc taataaagcg gaactggaaa
     5041 aacgaaaaaa tgttagccga agtttgtcac ttgatctata tgaccagtac cgcgtgatgg
     5101 aaaacgagaa tggcaaatgg cgtttcactt cccctaccca tacggttttg gcttttcaac
     5161 aagcgctgaa ggaactagag gcagaaggag gaatcgaaac tcgatattca cgttatctcg
     5221 ccaataatca tgtgctacga caaaaaatga cagagatggg gttccagcct ttcattcaag
     5281 aatatcaggg gccattcatc acctcttttc tttaccctaa ggactggcga tttgatttta
     5341 atcattttta tcagtattta aagcaaaatg gatttgtgat ctatcccggg aaaatcagcg
     5401 atcatgaatg ttttcgtatc ggaaatattg gcgagatcta tccggaagat attttggaac
     5461 tgacagcaat tatcaaagac tataaggaga acgttaatga ttaaaactgt aatttttgat
     5521 tgggcaggaa caactgtgga ctttggctgt atggctcctg tccacgcttt tagaaatgct
     5581 tttctagaaa aaggaatcca actgactgac aaagaaatcc gctaaccgat gggaaaatta
     5641 aaatgggatc atatccaaca gcttctttcc ctcccttctg tcaaaaaaca atggatagct
     5701 atttatggac aactacctca ggaaaaagag gtggaggaat tgtatcaaca atttgaacat
     5761 tatctgttta aagatttggt caagcattct accttgaaac ccgacacatt ggaaacgatc
     5821 gaacggttga gaaacgctgg gatcaatatt ggttccacga atggatttaa tgctcctatg
     5881 atgacaatcg tagcagaaac cgcaaaaaaa gaaaggtatt caccagactt catggcaact
     5941 tttgaagacg tcgaagggta tggtcgaccg tttccttata tgatccataa aaacatgcag
     6001 tacttcaaaa acaagtcggt tgatgaagta atcaaagtcg gagatacggt ctcagatatc
     6061 caggaaggga aaaatgccgg agtgttgaca gtaggtgtaa ttgaaggtag ttcgctaatg
     6121 gggatttctt atgatgagta ccaaaaatta gagatgggga aaaagatcgt ttaaaacagt
     6181 ctgttgctct tagattccaa caagccggcg ctgatatttg tgtaaacaat ttaaaagagt
     6241 taacagaata cattttgtac gctgacaaga atccagaatg acttttatac aaaataagag
     6301 gttgtgaaaa agctgtcctg tatcaagtaa taaaaacagc caagcaaaaa tagctcctca
     6361 tatttttcgt ttggttaggc ttattaccgc agatgcaagc ttttgaacac cgtttattcg
     6421 aaaaaaaaaa gggagctgtg acacgacttt tgtcacagtc cccttatttg caaacaaaca
     6481 gtgtttaaga gacaacctct ccactaaacg gtgttcattc agctgactct cggtatttgc
     6541 tcaaaaaacg ataaaatttg aaaagctatt ttctcttttg tttctctaat actcggatca
     6601 gaacgctgat ttcacattct ctttttctta catcttatct ggtgcatgaa gtcctagcaa
     6661 acgaaggtct tctttcaata atacagtcac tgcataaact agagctaagc gtgcttcttt
     6721 ttgttcatca tcagctaaaa tcttcgtatg agcatagtat ttgttgaatg cttgtgctaa
     6781 tttgattgca tgtttcgcaa tcactgacgg ttcgtatttt tccccagcag acaagaccgt
     6841 ttcagggtat ttttgaacta atttaaccac ttcccagctg gtatcgtcat tcaatgcata
     6901 gtctgctgtt tctgaaggga caaatcctgc tttttccaac aggctgactg cacgagcatg
     6961 tgtatattgc acataaggtc cagtttctcc ttcaaatcga acaacttctt ccaggttaaa
     7021 gtcaaatgta ttcagtcgat cgtttttcaa atcgtggaaa atgactgccc caaccccaac
     7081 ttgtttagct acagcatctt tgttttccaa atcagggttt ttctcgctga tttgttcttt
     7141 agcagattgg attgcttcat ttaatacttc ttctaataag acgatctttc ctttacgagt
     7201 tgataatttt ttaccacctt gcgtgatcaa accaaatgga atatggtgca tgtcgtcaga
     7261 ccagtcaaag cccatttcct tcaagacagc tttcaactgt ttaaaatgat agctttgttc
     7321 attccctact acatatagag attgtttgaa gtcgtaagta cgtttacggt acaatgcagc
     7381 agctaagtca cgtgtaatgt atagtgtcgc accgtctgac ttcttaatca acgctggatt
     7441 caggtcataa gcagataggt caacgatctc tgcaccttta tcctcgttca gcaaatgttt
     7501 ttcttctaaa agtttgacga tttcatccat cttgtcattg tagaaagctt caccgttcaa
     7561 cgaatcgaaa cgtacttcca gcagatcata gattttattg aattctttca tcgattcatc
     7621 acggaaccat tgccataatt gaatagcctc tttatcgcct tcttccagac gtttgaacca
     7681 agcacgagct tcttcattta attctggttc tgtttcagct acttcatgga attggacgta
     7741 taaacgaagc aattcattga ttggttctgc tttaacagct tcttctgtac cccatttttt
     7801 gtaagcgacg atcagttttc caaactgtgt tccccagtca cctaaatggt tgatgcggat
     7861 tggctgataa ccgatttttt ccataatgaa accgatcgaa ttaccgataa ctgtcgaacg
     7921 caaatgtccc attgagattg gtttggcaat attaggagaa gacatgtcga ttggtactgt
     7981 tccttgattc ccgatgttgc tatccccata atgttctttt tctttcacta cggtttgcaa
     8041 aacttttttg ctgataagtt ctttattcat gaaaaagttc aagtaaggtc cgacaacttc
     8101 gattttttca aagttggcag aatcgatttt ttctgctaaa tctgcagcga tttgctgtgg
     8161 cgcttttcgg taaacttttg ctaatgaaaa ggctggaaat gcaacatctc cgtgttcagc
     8221 tgattttgga ttttctagta attgctcgac ttgttccagt gttaaatcat ctttgacagc
     8281 gtcatagaca gcttttgcga caagatcttt attattcatc tttattcctc ctaaagtgtt
     8341 cccttgattg ttaaacaaaa aaaatctcta gcgacggcat acttagtatg ccatcactag
     8401 agacgagatt tcccgcggta ccactccaat tgtcctaaaa aaggaccagc tccatctgat
     8461 atcggtagac gaccgtctgt ttaaaacaga attctccaag tgcgcttcgt tgtatcttct
     8521 tcattcggct tccaccactt ccgaattcgc tagccaagaa tgatacaagt actctcttgt
     8581 tcaacaaatc ttagtcttat taaactttct tgaattatat cagaaaaaaa aacgaaaggc
     8641 tagtcgtttc ataaaaaaaa aatccagatg gcctaaagga gaagaaatac cacctggata
     8701 gctataaatc atttcgtaaa tacgaaagac ttaacaaatt aatcagctac gataatcgtt
     8761 ccttcttcag aagctaaaag attctcgata ttttctaaag aagtgatgat tgccttagcg
     8821 tttggttttg cttcaacaaa tgcaatagct gcttctactt tcggaagcat gcttcctgga
     8881 gcgaattgct tttcatcaat gtattgtttc atttctgaaa cagtaacagt ttctaatttc
     8941 ttttgatctg gtttttggta attgacatac acattgtcta ctcctgtcaa tacgatcagc
     9001 aaatccgcat cgatgatttc ggctaatttt tctgaagcaa aatctttatc gattacggct
     9061 tctcgtcctt ctaatccagt agctgtttct acaacaggaa tcccaccgcc accaacagag
     9121 actgtgataa ctccttgatc gaccagcgtt tcgatgacac gtgcttcttt aatcgagatg
     9181 ggtttagggg aagcgactac ttttctccat ccacgtccag catcttcttt gaatgtcaca
     9241 gtggagtcag cattcatttg ttctttggct tcttcttccg tgtagaatgg accgactggt
     9301 ttacttggat ttttgaatga aggatcattt tcatccacaa taacttgagt gactaaagaa
     9361 accacatctt tatcgatccc tttttccttc agtacttcgc ccatggcgtt ttgtaaccag
     9421 taaccgatag atccttctgt cattgcaaca catgtatcta aaggcattgc cggtgttttc
     9481 tcagaatcag cagcttgctg ctgaatcaat aggttaccga cttgcggacc atttccgtgg
     9541 gagatgatca attcatctcc ttgttcgata aatttcacta aatactttgc tgtttccatt
     9601 aacgcttctt gttgggcttt tgcacttgca tccgtagaca agatggcatt gccccctaaa
     9661 gcgacaacta cttttcgttt ggccatttca ttcgtccttt cttcagttct accgaatttt
     9721 ctagataaca aaacgagcca gcgttttgtg attttccctc aatgctgact cgttttttcg
     9781 tgagatgaat gtggttatgc ttaaacgcga gggataaaca agttacctaa tgttgctgcc
     9841 atgatcgctt tgattgagtg catacgattt tctgcttggt caaattgacg agcatatttg
     9901 ctgcggaaga cttcatctgt tacttccatt tcagtgatgc caaagcgttc tttcatttgt
     9961 tcaccgtaaa cagtgtttgt atcatggaaa gctggcaagc agtgcaagaa gatcaaacga
    10021 tctgtgttgt gtgttttttc taccatttcc atattgattt ggtaaggttt caacaatttg
    10081 atacgttctt cgaatttatc ttcttcaccc attgataccc atacatcaga ataaaggacg
    10141 tctgctccgt ctacaccttt atcaacatcg tcggtaatca tcaattgtgc accggatttt
    10201 tcagcaaagc cttctgccat tttgacgatt tcttcttcag gttgtaattc ttttggtgca
    10261 acgatacgca tattcgctcc taaaattgca cctgtgacta ataaagagtt agccatattg
    10321 tttcgaccat ctccgcagta agcaacagtg ataccttcta ctgttccaaa gttttcttgg
    10381 atagttaaaa agtcagcgat catttgtgtt ggatgccatt catcagtcaa gccattccaa
    10441 actggaacac cagagaattc tgctagttcc tctaccattt tttgactgaa tccacggaat
    10501 tcgattccgt caaacatacg tcctagtact ttagctgtat cttctgtgga ttctttttta
    10561 cctaattgga tatcatttgc acctagatat tcaggatgtg cacctagatc gatcgctgca
    10621 gtagtaaatg cagaacgagt acgtgtagat gttttttcaa ataaaagcgc aatatttttt
    10681 ccttctagat agtgatgtgg gataccacgt tttttcaagt ctttcaagtg ttctgaaaaa
    10741 tcgatcaaat attgtaattc ttcttttgta aagtcttttt ctgctaataa gcttcttcct
    10801 tggaatactg attctttcat attaaacatt ctcctttaga ttgtttttat ttaagattaa
    10861 agaggaaatc ccgacggcag ctgacagtaa gactgccgtc aggaaacaaa ttacttcaag
    10921 tcttcacgga caagtggttg gctcatgcag cgtggaccac cacgacctcg tgacaattca
    10981 cttgaattga tttctaacac tttgatgcca tagctacgaa gcaattcgtt cgatacataa
    11041 tttcggttat aagtgactac aacacctgga gcgattgcca atgtgtttga tccatcattc
    11101 cattgttctc gaggtgcaac gatctcatct ccattaccag ttgggatcag tacaagatca
    11161 tctaatccaa gagcagcttt catcgtttcg tgtaagttat ctgaatgagt aatcttgata
    11221 tcatcaccat ctggttcgat tgtaaagaca tccatcttac cgtctttact ttgaattgct
    11281 ggatggatag tgaatttatc gtgatctacc attgtaaata cggtatccaa atgcatcatt
    11341 gcacggttgt tcggaatttt gattgccaac actttttcaa atcctgaatt ttttgcgaat
    11401 aggtggcgtg ctaatttttc taaagctttc gcattagttc gttgagaaat cccaacagct
    11461 actactttat cgcttaagat caattcgtct ccgccttcga tatgatctgg attttcacga
    11521 tccaaccata cttcgattcc tttatttgca aatcttggat gatgtttcaa aatatattct
    11581 gtaaacatcg attcacgacg acgtgcttca aaagtcatct tattgactgt catcccattt
    11641 cccattgatg cggaagggtc gcgtgtaaag tacaagtttg gcattgggtc catgtaaaat
    11701 ggatagtcat cgtctgctga cagatcatat agactgctag aacgtacttc gatatctttt
    11761 gtacgaaccc cagccatgat tttatctacc atttcttgcg tttccattga tagaaggaac
    11821 tcatgaagac cctctcttac agcattcgat gcaatatgag attcatccaa cattttattc
    11881 aagaattgtt ctttgacatt gcctgcgtca atcgcttctg ccgctaattt ttccaagtaa
    11941 agagtctcga cgccttcatt ttgcaatgtc ttcgcaaagt tgtcatgttc ttcttgagca
    12001 atcggaagat atggaatatc atcgaacaac agacgatcca taatatctgg tgtcaaattt
    12061 tccacttctt gtccaggccg tttcagtaaa actgtcttta atttccctat ttcagagaaa
    12121 acgtgaatag gtttatccat gttgtgttgc ctccttcttt ttgattacat gtataggata
    12181 tcctcctttt gtaatcactt tcataatttt gtctcattca aaatgagaca ttc
//
LOCUS       DACDIE010000072        11943 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.072, whole genome shotgun sequence.
ACCESSION   DACDIE010000072 DACDIE010000000
VERSION     DACDIE010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.072"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(289..2073)
                     /locus_tag="IV250_002322"
     CDS             complement(289..2073)
                     /locus_tag="IV250_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8769570.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGVYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFVAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQEFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            complement(2073..3824)
                     /locus_tag="IV250_002323"
     CDS             complement(2073..3824)
                     /locus_tag="IV250_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8769571.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAAFLQYLFRYIWRTNIWGSAARLERELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQQLEAKIEGGEA"
     gene            complement(3966..4190)
                     /locus_tag="IV250_002324"
     CDS             complement(3966..4190)
                     /locus_tag="IV250_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HAP8769572.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            4441..6027
                     /locus_tag="IV250_002325"
     CDS             4441..6027
                     /locus_tag="IV250_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAP8769573.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            complement(6093..7868)
                     /locus_tag="IV250_002326"
     CDS             complement(6093..7868)
                     /locus_tag="IV250_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAP8769574.1"
                     /translation="MLNILNPLIDQLKKKHTYFILGIAVLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIHYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(8079..8981)
                     /locus_tag="IV250_002327"
     CDS             complement(8079..8981)
                     /locus_tag="IV250_002327"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAP8769575.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAFMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGAEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKVIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     ISMFKAQAFDPEVFPELLAEEVTL"
     gene            9260..11605
                     /locus_tag="IV250_002328"
     CDS             9260..11605
                     /locus_tag="IV250_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP8769576.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLLFAA
                     LGIGIGAGYFAYLVEDTQLPTKKELQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIVLSAQVEKHFSKNEIITTYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSEAYYQKAQQTIQNKGYTIHSTIDKDIYAALQTGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLVGSES
                     RVSDYATTWQEGKNAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINEKITTPFKDVISSLNGNLGNA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSQARIRSANYLANLINQAY
                     QANPEIFGTDEKFDLSSDVMKKKVSGFTGQTAGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANPETKESDDKDD"
BASE COUNT         3648 a         2438 c         2219 g         3638 t
ORIGIN      
        1 gaaaaaccca aaaagccgtc catggaacca tgggagactc atcaggttta gcttgcatca
       61 ggcaatcact atccaagttg tatcgttttc tttatattat cgaaagcaaa cgcttttgtc
      121 aacgctttct tagtggttat tagccgcttt tgactagctg tcgtcgtctt ttttaaatcg
      181 ggtgactacg ccactaacgc tcacagatga agaatctgcg aagctctgtt ttattttcac
      241 tctagcgctt agggcaaaat gctactatgc tactgtcaca acctcttttt acacaccact
      301 gctttgcagt ttgtacatat ctgcatacaa accattttgc gctagaagtg tttcatgatt
      361 cccacgctct actatatgac ctttgtctaa aactaaaatc aattctgcat cccgaatcgt
      421 ggacagccga tgggcaatcg ctatcgttgt ccgtccttgt cgcatttttg ccaatccttc
      481 ttggatcaat ccttcggttt cagtatcgat atttgctgtt gcttcatcca atactagtat
      541 tttaggatct gtcacgattg ttcgcgcaaa agtgatcagc tgtcgttgtc cactagaata
      601 gctggcaccg tgttcgatca cttttgagtg atatcctttt ggtaattcat cgatgaactt
      661 gtttgcttga acaaactcag ctgcatggcg aatctgttcg tctgtgatag ctggattcat
      721 catccggata tttcctgcaa tatctccata aaacaagaag gcatcttgca agaccaatcc
      781 cattttctta cgtaactctt ccattggata ttctcgaatg tcatgatcat caatcaagat
      841 ttggccttca taaaattcgt aaaaccgcat caatacgttg atgatcgagc tttttccgct
      901 tccggtgtgc cccactaatg caacggtctc acccggatta gcgacaaaag agatattgtt
      961 caatacattg tgctctccat cataagaaaa gcttacattg cgaaactcga tcttacctga
     1021 agtaatcgct tgatctgctc caggattttg ttgcggtgca tattcttctg tatccatgat
     1081 tttcaaaata cgactaccgg caaccatccc atctgtaaag gtgctcaaaa agtccatcat
     1141 ttgagacatt ggattaaaaa attcctggac ataggtcgta aatgcataga tcagtccaac
     1201 ttctacggga gaatgcaacg catcgatccc aaacaaagtc aacgccaacg caatcgccaa
     1261 tgcatagaga aaattgatga ttggacttaa cagaatcgaa ttcgttttga tcatcgcata
     1321 gcgcgtgtct aaatactcgt tgttcacttg ttcaaattct ttagctaaac gagcttcttg
     1381 gcgaaactgc tggataatac gcatgccgga aatcgattca ttcaatttcg tgttcagctg
     1441 acttaatttt tcccgcatat ttcggtaaat acgagaactg aatttctggt aataccagat
     1501 gacgattcct aaaatcggca agaacaccaa agtccacaag gcaatttctc cattgatttg
     1561 aaacatggca acgaaagaag aaacgactgc aaagatccca gtcaatacca tcaaaaagac
     1621 ataccaaaat tcaaatagcg tttctgtgtc attcgtcact cgagaaacgg tcgatcctgc
     1681 cggtgtctgg tcaaaataac gcatacctag tgtatgcagc ttttcaaata atcttacccg
     1741 aacatactga taggtcttca atgaagccat ggaatacaaa taccattgga aaaaccaaat
     1801 gatacttttg actatgactc caaacaagta cacaccagca aaaaacaaaa taacttgtgt
     1861 cgtagctgac tgtttagcca gatgatcatc cataaaagtt tggatgatcc ttggcaatag
     1921 gacattgatc acagacaggg caaacgcaaa taagatagca acgatgaacg ttcttctaaa
     1981 aggtttggcg aacttcatca gtcgtttgat gatcttcttc tgttctttaa gtggaatcga
     2041 ttttgtccat tccgattgat aattttcatc catcaggcct cgcctccttc tatcttcgct
     2101 tctaattgct gtttttccca cattcgttca taccagccac ctaatgcaag cagctcgtgg
     2161 tgggttcctc gttcaatgat tttcccttga tcgacaacaa tgatttcttt tgcatgcatc
     2221 acgctgctta aacggtgagc tgcaatgatg gttgttttct cttttcttat ttcttttaaa
     2281 ttatgcaaga tagcttcttc tgttttggca tctactgcag aaagtgcatc atctaagatc
     2341 agcaattcag gatttttgat caatgctcga gcaatcgaaa tcctctgttt ctgtccgcca
     2401 gataaagaga ctccacgttc tccaactaag gtatcataac cttttggcaa ttccttgatt
     2461 tcattgttga taaatgctaa agaagccgca tgctctactt cttcttctga aagttttgga
     2521 ttagaaaaac ggatattatc tcgaatggtc atcgaaaaca ggaagtgatc ctgaggcaca
     2581 taaccgattg atcctaataa agcattcaat gaatattctt tgatcgaacg tccgccaaat
     2641 tggattttcc cttgataatc atcgtattca cgcatcagta atttgatgat tgtcgttttt
     2701 cccgcaccag ttttcccaac aatacctaat gtttctcctt cttggatcgc aaatttgatt
     2761 tgttctaatg ctggtgattc ttctttcgga tagttaaaag aggaaatagc tacattcagc
     2821 tcgccttttg ccggggtctg cactgcaccc agttgttcca cgatatgcgt cttttcatgc
     2881 aaaagttcat tgacgcgatc ataagaagca tttcctcttt ccaatacgtt gaacaatcga
     2941 ccaatcgcaa acatcggcca aacaagcata ccgatatagc taataaatga gaccatctgc
     3001 ccgattgaga tcgtgccttc caagacatac tttccaccta aaataattgt tgcaacataa
     3061 gacagaccga taatcaaggt aatgaatgga tcaaataagg agtctaaaaa attgactcgt
     3121 ttgtttttaa taattgcatc ttcaatcttt tcttcaaaat ccgcgagatc ttctttttct
     3181 tgtccaaatg ttttaatgac tttgatccca gtgatacttt cttgtgtctt atcattgata
     3241 ctagaaaaag cttcttgcga atcacgaaaa gcatcgtgga gttttgaccc cagcactcgt
     3301 gaagtaacgg ctagcaaagg cagaggaatc agcgcaatca gtgttaagcg ccaatcaata
     3361 aacaaaatca tcgcaatgat cgttgttccg cccgtaatca ctgaatctgc aaaggtcaag
     3421 attccagctc ctgctacatt ctggattgca ttcaaatcat tcgttgcatg agccatcaaa
     3481 tctccagtcc gatatttttg gtaaaagaga ttgtccattt ctgtaaaatg tttgaatagc
     3541 tgctgccgaa gttctctctc caaccgagct gcacttcccc aaatattcgt ccgccagata
     3601 taacgaaaaa gatattgtaa gaaagctgca aggatcaata ataagatcca ccaggaaatg
     3661 atctttattg ataaattctt atcggcgatc tcatcaatga tgatcccaat cacttttgga
     3721 ggaactaact gtaccaaggc cactgcgaat agggagaaaa tcccaattaa ataatgtttc
     3781 ttttcctgac gaaaaaacca acctaatttt ctaaaaatcg acattttact cctcctttgt
     3841 ctttatcaag agcataggat tgtctgtgct tgtctttttt gctgaatagt ctgaatagtc
     3901 taacaaatta cgaaataaaa aacggactga aaaaatcagc ccgcaaaaag tctttgggtc
     3961 agaacctatt ttttcccttg gttcttcatt tgctgcatca tctggcgaat tttcttctca
     4021 gatggttttt gccccattga catcatcatt gatcgcaaca tgtcctcatt aacgggagga
     4081 tttttttcta aatagtcttt catatattta cgcgctagga aaaagccacc tactgcgccg
     4141 attagcaaag caataatagc aataagtacg acaatacctg ttgataccat ataaatctct
     4201 cctttctatt tccgaattgc tctcaatgat ttattttact aaagtttatt gaaaaaagaa
     4261 aggcgtattc tttagtttca tgcaaaaaat agtggttttt ttagagaaga acaaaatttc
     4321 tttcattaac cagcaagaca caccacgaaa aaaaattagg cataccgaat ctttttcttt
     4381 aattttttct ttctgaggtg tagaatgtaa tcgtagataa ataggaaagg tggttttttc
     4441 ttgaataatc aatccaacaa acaatttcat aagcatcgac ttattgaaca tacaaatggt
     4501 ttatctttag ctgaagtaaa tggaacagtg gatgtaccaa agggaaaaag tttttttaaa
     4561 acacttcttg cttactctgg accaggtgca ttagtagcag ttggttatat ggatccaggt
     4621 aactggtcca cttcgatcac tggtggacaa aatttccaat atttattgat gtcagttatt
     4681 ttgatgtcta gtctaattgc tatgctttta cagtatatgg cagcaaaatt aggaatcgtg
     4741 acccaaatgg atttggccca agcaatccga gcacggacaa gtaagacatt gggtgtcatt
     4801 ttatggatct tgactgaatt agccattatg gcaacagaca tcgcagaagt cattggtgca
     4861 gcaatcgccc tctacttact ttttgatatt ccattggtga ttgccgtctt tattactgta
     4921 ttagatgttt tcgttctctt actactgaca aaagtcgggt ttcgtaagat cgaagcactg
     4981 gttgtctgct taattttcgt cattttattc gtttttgtct atcaagttgg cttgtctaac
     5041 ccagattggc acagtttatt tgctggcttt atccctaaca gtcatacatt ctcagactca
     5101 ccggtcatcg gcggcgaaac tccgctgact ggagctttag ggattattgg tgcaacagtc
     5161 atgccgcata acttatatct tcattcagca gtttcacaaa ctcggaagat cgatcaccaa
     5221 aacgaagaag atgtagcaca agctgtacgt ttttctactt gggattctaa tatccagttg
     5281 tcaatggctt ttctagtcaa tgcactgcta ttgatcatgg gtgtcgctgt cttcaaaacc
     5341 ggttctgtcc aagatccttc cttttttggt ctgtatgaag ctttatctga tccatcagct
     5401 atgagcaatg gtattttgat tgctgtagca aaatctggta tcttgtccac tttatttgct
     5461 gtagcacttc ttgcttctgg ccagaactct actattactg gtacgttgac cggtcaagtc
     5521 atcatggaag gattcatcca tatgcgtatc ccgatttggc taagaagact ggtcacacga
     5581 ttgctttctg tgattcctgt attgatttgt gttttgttta caagtacaaa aggaacgatc
     5641 gaagaacatg ccgctttgaa taacttaatg aatgaatccc aggtgttctt ggcttttgcg
     5701 ttgccatttt cgatgattcc tttactgatg atgaccaaca gccaagcgga gatgggtagc
     5761 cgatttaaaa attcattcat cgtaaaactt cttggctggt tctctgtgat tagtctaact
     5821 tacttgaact tacgaggatt accaggacag atcgaaagtt tcttcggcga gcaagccact
     5881 tccgctcaac tagcattggc tgatcacatc gcttatgtaa tcattgttct tgtccttctc
     5941 ctgttggcat ggacgattgg tgatttatat aaaggaaaca aaaaatatga acagcttttg
     6001 caggaaacgg tagaagaaat cgaataaaaa ctgatttttc cacacaaaga cccttgatag
     6061 ttaggttaaa aatctagcta ccaagggtct ttttatttag aagataaagc ttttttcaga
     6121 tttaatttac tgaaaactag catgcttgtt actacgatga attgcgttaa tggcacgcct
     6181 gcaccagtgt agacacttaa aaaatagtcc tttttttctg gcgcataaga acaaaaccat
     6241 gagacaccga aaagaaaaac caattgtgtc agtattaatc cggtgaccac aatcaaagag
     6301 gaactataat aggcacgatc agcttttgca aatacggttc ctagtatcat aagtaatatc
     6361 cctgctattg aagcaatcag cacgggaata gccagaactt ggtaaatttt tatgatgaca
     6421 ttcgcgactt ttgctgattt tgttaattgg aaattaggtg ttctcggttc attactatcg
     6481 atccaattct ccattagcgg aaggtttaat aattcttgag cctgtttgac ttgttcttgc
     6541 gttccgaaac tataatttcc tccttgatgg aaatctttat atcgccatgc ataagcgtat
     6601 cctgattgaa ggaaatcgaa tacgattggc agatcactca tcgtctttcc atctcctgat
     6661 cccatcagat agaactcttt tttctttttg atggttccct tgtgataggc ttcttctaat
     6721 tcttggttga cttttttcca aaaatcattg gtttcttttg cattgttttg gtaataacca
     6781 gattcgctta ccacatctcg caatgcccaa aagatgatat ctccgggtaa ttcttttact
     6841 tttccacctt gagtccacgc gtgagtcgta tataattcgt caatacgggt agaaacagta
     6901 tggaatgtag gagatgcggc taatgcttta tttagtgcag catgtgagac ccatatctca
     6961 ctgttttctt tattcaagtt cgtttcatca tctattcgga tcagttgttt catcaaacgt
     7021 ccaaattctc cactggaacg gtcattgatg gttgcaatac cgtatgttgt ctcattttta
     7081 tattcgatcc actgatagac gatggaaaag ccaactatag gaagtaaaaa caatgaaagt
     7141 ttcagccatt cccgtttcga gagaagaaag agtcgttttt ttaacttctt tacttctttg
     7201 aaagaaaagt cttgccctag caagatctgg acgaaactga tacccaaagc tactacagta
     7261 aaaggaagaa gccagataga atcttcgcga atgaaccaaa agaaaggaaa agcgacagaa
     7321 agccctaaaa accatggcag caattttctg ctagaaccgt atttgcggta ataaatagct
     7381 aataaacaag cgataatgat ctcaacagcc ggaatcacga gggcattacg atatgttcga
     7441 gtggaatatt catgtgtaaa actaatcgga gaaaacaaaa aataaaaata gatacttaaa
     7501 agaagatact tgttcttcac aatcggttta aacgcgagtg caaccaatat actggcaaaa
     7561 atactgaaca tgccgaacaa aactgcataa gaaagatgca gtttatgtgc aagtactaaa
     7621 aataagctgt aggagattcc tttggaaagt gttgtagtcg tatactcccc tagccattta
     7681 ccatccaaaa tattttgagc atagtgaatc agcagctgat catcataggt cgccgacata
     7741 tctgttccat agattgcttt actatataaa tacaaccgaa taattgtaaa taatacggct
     7801 atgccaagaa taaagtatgt atgtttcttt tttagttggt cgatcaaggg gtttaaaatg
     7861 ttcagcatct tttcgtcctt tcaatcacgt tttgctactt gtttgtgaaa cttttcgttt
     7921 tttacttttc tatcagtata ccctgtttgt ctgaatttaa aaagaggggg caaaaggagc
     7981 atggcgaaaa cgaatgcaaa aagcactcct ttgtttctta aacaaaaaag gacaaggcag
     8041 actgcccaat ccttttttgt tgctgtcagg tagaaaactt aaagtgtgac ttcttctgct
     8101 aataactcag ggaacacttc gggatcgaat gcttgcgctt tgaacatgga aatttctttt
     8161 ccatatggtg cgtaagcttt ctttttatct ttaccgacat atggggtttc taatattttc
     8221 gaaatgttgt caaacattgg atcatggacg attttgttca atgtgtcaaa tccaattgtt
     8281 ccaaaaccga tattggcatg ccgatcttta tgagaacctt gtggattttt agaatcattg
     8341 atatgcagta ctttaagccg atctaatccg atgaccttat caaactcttc cattacacct
     8401 gaaaaatctt cttttatatt atatccggca tcattcgtat gacatgtatc aaaagtaacg
     8461 gataattttt cattcaattt tactccatcg atgatttgcg ctaattcctc aaatgttcga
     8521 ccgatttccg tacctttccc tgccatcgtc tccaaagcga tctgtgccgt ctgttctttt
     8581 gtcaaaactt cattcaaccc ctcaataatt ctagcaatcc cagcttctgc ccctgctcct
     8641 acatgagcgc ctgggtgaag ggtgatttga gtagcaccta aagcttccgc acggacaatc
     8701 tcttctctca gaaattgtac agcaaatgga aacatttctg gtttgatcgt atttcccaaa
     8761 ttaatgatat aaggcgcatg aacgacgata tttgttagtc cgttttctgc cataaatgct
     8821 tttccggcat caatattcat ctcttcgata gcttttcttc tagtattttg aggagcgcct
     8881 gtatagatca taaaggtcga agatccgtaa ctggctgctt cttctgccgc gcctaacagc
     8941 attttctttc cgctcatgct gacatgtgag cctaataaca tggatggatt cctccttgaa
     9001 aggcaattgt ttcgaacaat tcctgtatat tgataagatg atgtacaaag aaaatggttt
     9061 gtttttcttt gccctgttct ccatagtaac tgaaactttc cctaaagaaa agcttaagaa
     9121 ctctttcata aaaaagatca tttcctcacg ttttatctca ttcgaaaaag aaattcctgt
     9181 caaaaacagc cttgtctgtt tttttgtcct attatttgct ataattagcg aaataaaacc
     9241 attagttgag gtgacccttt tgccacgcaa aaatactcga aaaaaacaga atagaagaaa
     9301 aaaagaaaaa tggtttgttc ctaagattat ttttagagtg tttcaatcat tgactgtttt
     9361 tattactgtt cttcttttgt tgttcgcagc tctaggtatc ggaataggtg ctggctactt
     9421 tgcctatctt gtcgaagaca cacaattgcc gactaaaaaa gaactgcaga cagaactagg
     9481 aaacattacg gaaacgtcaa agattgttta tgcagataat acagagatat caaaaatcca
     9541 gacagatctg atgcggacga ctatttcatc tgataagatt tcgcctttat tgaaaaccgc
     9601 gattatcagt acagaagatg aatacttcga taaacatcaa ggttatgtcc ctaaagctgt
     9661 tttacgagca ctagtttcag aagcgactgg catcggctcg tctggaggat ccactttgac
     9721 acagcaatta gtcaaacaac agatcctaac cgatgaaaca acattcaaac gtaaagcaaa
     9781 cgagatcgtt ctttctgcac aagtagaaaa acacttttca aaaaatgaga tcattactac
     9841 gtatttgaat gtctcaccat ttggtcggaa taataagggg caaaatattg ctggagtcca
     9901 ggaagctgct agaggaatct ttggtgtaga tgcaaaggat gtgactctgc cacaagctgc
     9961 ttatatcgct ggtttgcctc aaagccctat cacgtatagt ccatacacga atacaggcgc
    10021 gctaaaagaa gatctttctg ccggtcttgc cagaaaagat tttgttttat tcagtatgta
    10081 ccgtgaagga caaattacga aagaacaata cgaagaagcg aaagcctatg atcttacaaa
    10141 agactttctc ccacaacaaa tcgcggaaca gactgacaga gaattcttgt attataccgt
    10201 gatgaacgaa gcaacaagga ttattgcaca acaattggca gaaaaagata atgctgatat
    10261 gtcagattca tcggtcagtg aagcttatta tcaaaaagca cagcaaacga tccaaaacaa
    10321 aggatatacg atccattcga cgatcgataa agatatctat gctgcattac aaaccggcgt
    10381 agaaaattat ggctacatgt tagatgatgg tgctggtagc caagtggaga ctggaaatgt
    10441 attgatggat aacagaaccg gacgtatcta tggcttcgtg ggtggtcgga attacagcca
    10501 gaatcaaaat aaccacgcct ttgatacaga acgtcaagct ggttcatcca tcaaaccggt
    10561 tcttgtttac ggtcctgcaa tcgatatggg acttgtcgga tcagaatccc gtgtatcaga
    10621 ttatgcaacg acttggcaag aaggaaaaaa tgctggtgag aaaatcgtca atgcgaccaa
    10681 tgaagggtcg aacaccttcc aaaccgtccg tgagtcactg gaatggtcca acaatatccc
    10741 agcttatcac ttatatcaag atgtgttgaa ttccggtgga tcgaaacaat acgcttacga
    10801 gcactacttg gcgaagatga actacccagc aaatgataat tggggcgttg aatcggctcc
    10861 tttagggaca gtagatgtaa cgacgcttca acagacaaat ggattccagg ccttagcaaa
    10921 tggtggagtc tacgaagaag gctatatcat tgattccatc acagataatg caggtaatgt
    10981 gatttataaa catgaaagta attctgtccg tatttattcc gaagcaacgg catcgatcat
    11041 gaatgacatg atgcgtagtg tgatcaatga aaaaatcact acaccattca aagatgtcat
    11101 ctcaagctta aacggaaatc taggaaatgc agattgggtc ggaaaaactg gttctaccaa
    11161 tgaataccga gattcatggc tgatcgtctc aactccatct atcactatca gtagttgggc
    11221 aggacacgat gacaatactg gaatggacag ccaagcaaga atacgttcgg ctaattatct
    11281 tgccaatcta atcaatcaag cttaccaagc aaaccctgag attttcggca ctgatgagaa
    11341 atttgactta tcatccgatg tgatgaagaa aaaagtatct gggttcacag gacaaacagc
    11401 tggaaaagtt actgtcaata aaaaatccat ccaaacgcca ggaaaaacag tggagtcact
    11461 ttgggcaaaa aatggtccga agaaaagcgc ctttaaattc ggcgtaggcg gtactgatga
    11521 aaactataca gattactgga acaccgcttc agcttatgct agagcgaatc cagagaccaa
    11581 agaatcagac gataaagatg attgatctaa tagattaatc agaattaaag ggagctgcga
    11641 caaaaatctt gtcacagctc cctttttccg aataaatggt gttcaaaagc ttacatctac
    11701 gatattaaga caaatttatg taaaaatgtg aaaaaccaga gagtataaaa tattttgtgt
    11761 aaatgaaaaa atccatacaa aaaaggaagt cccttctgta gaataaagtt aacgacaacc
    11821 aattcacaga aaagaggact tccctatgaa tgattttact acagaaattg tgcaaactct
    11881 agtcactaaa ggcgatttaa atgaattatt ccgttcgcac ttagaaaaag cgataaacac
    11941 act
//
LOCUS       DACDIE010000073        11792 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.073, whole genome shotgun sequence.
ACCESSION   DACDIE010000073 DACDIE010000000
VERSION     DACDIE010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.073"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..184)
                     /locus_tag="IV250_002329"
     CDS             complement(<1..184)
                     /locus_tag="IV250_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769577.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISG"
     gene            complement(200..814)
                     /locus_tag="IV250_002330"
     CDS             complement(200..814)
                     /locus_tag="IV250_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAP8769578.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(961..1644)
                     /locus_tag="IV250_002331"
     CDS             complement(961..1644)
                     /locus_tag="IV250_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP8769579.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(1753..2211)
                     /locus_tag="IV250_002332"
     CDS             complement(1753..2211)
                     /locus_tag="IV250_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAP8769580.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(2295..4058)
                     /locus_tag="IV250_002333"
     CDS             complement(2295..4058)
                     /locus_tag="IV250_002333"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAP8769581.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(4279..5739)
                     /locus_tag="IV250_002334"
     CDS             complement(4279..5739)
                     /locus_tag="IV250_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769582.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(5947..6873)
                     /gene="glsA"
                     /locus_tag="IV250_002335"
     CDS             complement(5947..6873)
                     /gene="glsA"
                     /locus_tag="IV250_002335"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAP8769583.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(6896..8323)
                     /locus_tag="IV250_002336"
     CDS             complement(6896..8323)
                     /locus_tag="IV250_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8769584.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(8354..9568)
                     /locus_tag="IV250_002337"
     CDS             complement(8354..9568)
                     /locus_tag="IV250_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAP8769585.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            complement(9909..10151)
                     /locus_tag="IV250_002338"
     CDS             complement(9909..10151)
                     /locus_tag="IV250_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP8769586.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(10183..10740)
                     /locus_tag="IV250_002339"
     CDS             complement(10183..10740)
                     /locus_tag="IV250_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP8769587.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(10753..10941)
                     /locus_tag="IV250_002340"
     CDS             complement(10753..10941)
                     /locus_tag="IV250_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP8769588.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(10954..11520)
                     /gene="amaP"
                     /locus_tag="IV250_002341"
     CDS             complement(10954..11520)
                     /gene="amaP"
                     /locus_tag="IV250_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP8769589.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
BASE COUNT         3455 a         2446 c         1932 g         3959 t
ORIGIN      
        1 atccgctaat cgtttgatct ggcatttcta tttccttacc atctaaagta acagtagtaa
       61 cgacaaaatg gtcttgcaat ggcggaactt ctaagtactg cccttgcaga aaatcattca
      121 ctgtttcata ttcttttttc atttcttttg aatctgtcga tggtttatag cttattttta
      181 acataactag tgctcctttt tattcgggac gtgcaatcgt gaaaatttct cctattgctg
      241 cataatcatt tcctgccaaa cggcttacag cattcaattt tcttggatcg atccttcctg
      301 tcttatcatc atagactgtt tcatttaaat gatatttctg tacttctagc aaaaataaat
      361 cagctgttac tttattttta ttccgtatct cgacatgttc atataaggat gtttcaaaac
      421 gaacttttgc ttctgtcaat gctggtactg aaatgattac tgaatcagtt aaagtcaagt
      481 cagtcaggta cagttcgctt tcctcattag gcagattggc tgctgtttta tttacttctt
      541 ctacgttgtc ctcatcaagg atatggataa cagcttctct tgtctcaaga atattctttg
      601 ctgtgtcttt tactgcatct tgttttctct ggatcgccaa ggataagatg ggtcgattcg
      661 aactgacgat attgaagtaa ctgaagggtg cgatattcac tatatctttt gctgatttag
      721 tcgttactac tgctactggt ctagggatga ctgaaccaat cagcaacttg taattttcac
      781 gttctgtcaa ttctgaagga ctccgttcaa acatggtttc gccttctttc tataaaattc
      841 gttaaaaagc agatcttgaa gacaaaatga aaagtaatgc ttttttcatc ttcttagaat
      901 ctgctttaaa ctatctatca atttttcttt tttgtcaaga atctgtgccg tgcgtgtttt
      961 ttagttattt gctttacttg cggcttcgac atatctttta gtgatcaatt gcggacgggt
     1021 gataatagat ccaactacga ctgaaaaagc acctaattca tatgctttct ctacttgttc
     1081 aggagagtca gtatgtcctt ccataattac tgggatatcg acttgttcta tgatttgatt
     1141 caataaatca aaatcaggtg tctgcgtctt tcgtgaatat tctgtatagc ctgctaaagt
     1201 cgtagacaaa agatcaaaac catcttcttg cgcttgaagc gcttcctcaa aagtcgaaat
     1261 atctgccata gctaaccgac cgtgttcgtg aatcatttgg acaagttcag cagttgtttt
     1321 ttctgtcgga cgtttacgag aagttgcatc cagtgcaatg atttctgctt gcgtctccaa
     1381 aagttcttcc acttcttttt ctgtcgcagt aataaacact tctgacccat cgtaatctct
     1441 tttgataatc cctattaccg gtaaatcaac gattcgcttg atttcattga tatcttctgt
     1501 cgtatttgct cgaataccaa ctgctccgcc ttcttttgca gccaaagcca ttctccccat
     1561 aataaaagaa ctgtgtagcg gctcgttttc caaagcttga caagaaacga cgactccgcc
     1621 tttgatttgt tccagtgtat ccacaatatc tcctccagta atgttatata aaaatacagc
     1681 ttcatataaa aatgtaaccc ttttctattc aggagacaat aatgagctat ttctttcagc
     1741 agacctttct tactaaaaga aaattacttc aaattcttcg tatacgacca gcttctcttc
     1801 tgtaaattct atgttcaacg cttctttata agcatgttta aaaaactcag catggacttt
     1861 ccgccaataa gctaatgtcc gatccccttc tccttctttc caagcatgct ctttcgtgac
     1921 ttcactaaaa ggcaagatgg tcacatttat atttttgatg atacaaactg gttcattttt
     1981 tccattcagg accacgtcta attgaccaac ttttggtaaa ggctcattct cttgtttata
     2041 cagatcgtaa ccacttgatg taccagtctt tatctttttt acaactagtt cgctcaactc
     2101 gtctgccatt ttttctgtat ccccaaatga aaagaaacct aatttttctg attgataact
     2161 aggatatttt tcttggaact ttttgacata ttcttctact tgctgataca taacatccac
     2221 ccctttgctt tttataatga aaaaaccatc cagatactag tataaacaat tatctggatg
     2281 gtgagcataa tgaattattg aatattcaag acacgcagac tatcttcaac gatttgtttt
     2341 ggtgttaaat gataacgttc ataaagctgc cctacaggaa cgctatccgt gaattctttc
     2401 tctgcaccaa aatttaatac gttcattgaa gaatcaccat aataacgact gatattttcg
     2461 ccaaatcctc ctgacaaact accatcttct aatgtgatga ctaattcgtg tgtttctttt
     2521 aatttatcta atgtttctac gtcattttct gtgatcgttc ctggattgat caacgttgct
     2581 tcgattcctt tttttctaaa agcttttact gtttcttttc ctaattcaag aaaaccgcct
     2641 aaagctaaaa ttgctacttt tcctccatta tttactgttt gataggctgg aatactataa
     2701 tcatttgcga tagctgtacc atgttctaca ccatgtgtag gaattcggat tgctacaggc
     2761 tgcgctgttt gttcgaacgc ccaatctagc atagagatca tatcctcttt tgttgctgga
     2821 gcaagatatt tgatgttcgg gatacttgat aagtaaggaa tgtcaaatgt tccttggtgt
     2881 gttcggtcac tagctgagat cgttccgcca ccgactaaaa taatcactgg attctcgttg
     2941 atcgctaaat catgtgataa ttgatcatat gcccgttgca agaaagtact tgattggaag
     3001 acgactggac gtactccctg agaagccaaa gcagaagcga aagtgataga gtgctgttca
     3061 gcgatcccca cgtcaaaata atgatctgga tgtttttctt ggaatgtttt cagtccaaag
     3121 gcgcctggta tcgctgcatt gactgcggca ataggagctg tacttgtttg cattttctta
     3181 tccaaataat ccatgatgat catattaaat gtttctccag aagttacttg ctttgcctcc
     3241 ccggtggaca gatcaaatgg tacatgccaa tgatatttca ttttattatc gattgccggc
     3301 tgataaccgc gacctttttc cgtatggacg tgaagtacaa tcggatgatc aatatctttt
     3361 acttcttcaa ataaacggat caccgtttcc agatcgtttc cattatccac gtaacgataa
     3421 tcaaatccca ttgctttaaa taggttattg ctgcttttac cgttggtttg acgaagctca
     3481 gctaagtttt tatataaact gccatgattc tcatcaatag acatctcatt atcgtttact
     3541 aatacgatta gattggattg taaaacacca ccattgttca atccttcaaa agctaagcca
     3601 ccacttaggg agccgtcacc gataaatgct accacatttc ctgattcttt cttcagatct
     3661 ctcgctttag ctaaacctgt tgctaagctg atagatgtag atgtgtgccc cactgtaaaa
     3721 tagtcatgtt tactttcatg ttggttggaa tatccgctca cttcacggta atgtcctttt
     3781 tcaaaaccat tttttcgacc agttaaaagc ttatgaccat aagactgatg ggaaacatcc
     3841 caaacgattt tatcctttgg tgaatcaaat acataatgaa acgcaatcgt taattctact
     3901 acacctagat tcggaccaac gtgcccacct acatcgctgt ccttttgcaa gattttttgg
     3961 cgcatctctt ctgccaactg tttcatttcc tcaacagata attttttgac atctgcgggg
     4021 ctattgattg catccaatac atgtaatact gtactcataa tacattctct cctccgtact
     4081 atcttctttt aatgaaaata gatttgttac actttatcta ttctactact tagagttgac
     4141 tctaagtcaa tagcatttta aaaaaatttc agagaaagct tgttatataa gtagatcttc
     4201 gcacgaatca ggactcaaat aaaaaccttc tacagacaat taaaaagctt tgtagaaggt
     4261 ctcctaatta aggtctcatc aagaaatatg tttttttaat aggtgaagtt cctgagcaat
     4321 atgatacaaa tggatgtttg caccttttaa tgagattgga tagatctttg tcttttcagt
     4381 ttctaatcca ttttgatagc tagcagcatc gcacaaaacc actctttttt tcgcacatag
     4441 acattcatca ggcaattctt ctaatgaacg aaataaaata ggatttattt cagcgatgcc
     4501 ttcttccgca tacaaatgaa attgggttcg ataaaaaaat tgtttgactg aactttctga
     4561 aacaacaaaa atttctattt cctgtctccc cgcttgttca ctgttgatca aagaaagact
     4621 tttgacgaat tttcgaatga aattttcatt ccatcttagt tggatttgat ttttcttttt
     4681 ccactcttcc aatatttcta caatttttcc gtacagaagt tgttcttttt ctgatagaag
     4741 atagatcctt tccggtaaaa atacttgtcc atctgcccac aacgtacgga caaattgtaa
     4801 aaaggattgc ttgaaaataa aattttctgc atcgtcgatt tcagcgatac taataatctg
     4861 taaaatcaat tcattcagtg ataggtcatt gagaacaaaa tttttataaa caacttcgaa
     4921 atcttttctt aacagctcca tgttctcatt ggtataattt ctactgttga acaaagaaaa
     4981 aatataatag acatcttctt cttgtaagtc tagaattcct ttttgctctt ccaatccttg
     5041 acagactaat tgaaataacg gcttcgtttc tgcgcttttc ttatcttctt ctttcagtcc
     5101 aaggggaaat gcctttcttc cgattgcgat ctctattcct cgtatgataa aggacttttc
     5161 atcttccgaa tatcttcttt taaaaaaatg agtctctaca taatcaatca actcgttaca
     5221 agcttcttct acatacggat ttttattttc atagccttcc caattgacat aacgtactaa
     5281 agctagcaag atgttccgcg agtcaaattc tgggataatg atttctttat ttttcaaata
     5341 atcattttct ttaaaaaaat ccaatacgat ttttttgatt gtatatactt tgctcaaaga
     5401 aataaattca agatcagcca gttctgtact cgtgaatata ccattaaaat aataacttaa
     5461 aaactttaaa aaataagatt gaccgtacag aaactgtgtg agctcagaaa gagcaatacc
     5521 atgtttgaat acgagtttat acttatgctt ctctcttact tgttctttta tacaatattc
     5581 tagacactcg gctagacttt ctagatcgtg aaggatcgtt ggtgtagtca cccttagaga
     5641 aatagctgtt tgttcgatgt ctatttcttg gaattcaaat aattgttcac acagatacac
     5701 ttttctaaat atatttcgct cgatgtaatt ttccagcatt cctgatacac gcctccagtt
     5761 tttcttatga ttttatttaa tcatatctgt aaatcgtatt tttgtttcct ttttatttag
     5821 aaaagccctt tcaaaaacca ataaaaaaag aaactgggac aaaagtcaaa caagctcaca
     5881 atttcagcct cattgtaggt agctttgagc cgtttggact tttaccccag cctctttcaa
     5941 gaatggttat tcaaagatgt cagcgtctag ttctttgact acatctttca acaattggat
     6001 accagctgcg ctgttaccaa atggatctag tgccgggctg aagacaccta tgccgtaacg
     6061 atttggcaca gctgccatca acccgccgcc tacaccgctt tttgcaggta caccaacatg
     6121 gacggaaaac tctcctgatt catcatacaa accagcagtt gtcataattg atttgacgat
     6181 tgtagcactt tcttcagtaa tgatttgttt gccattccaa ggagcaattc ctttatttgc
     6241 cagcacagca gctaattttg ctatgcctgt agcggtgaca ttgagggagc attgtctgaa
     6301 ataagcatcg agtatctccg gtacgtctcc ttcgatcatt tgattacctt tcatatagta
     6361 agcaagagag cgattgatat cacctgttct tgattcagaa tgatagattt cttcattcaa
     6421 tgtgatatta ggatcatcac agatctcttt cataaatgac aagattcgct caaatttttc
     6481 atctgcatct ttaccggcaa tcaacgaggt tgttgcaatc gctcctgcat taacaaaagg
     6541 attcattgga tgtttgcgat gattgatttc catattcatg atagaattga aggcaaaacc
     6601 ggttggttcc gtgttgattg tttgaaagac tttttctatt ccgttgtctt tgattgctaa
     6661 taacaatacg ggaacttttg acatgctttc gatggcaaag cgcacatcgg cgtctcctcc
     6721 ctcaatcagt tgttttgtat ccaaatcata aatcgccata cctaattgtt tgggatccac
     6781 ttcagctaat gctggaatgt aatccgccac ttgtcctgtt tgataatagt ggaaattttt
     6841 tccaatcaat tgttctaatt cttttacttc catttgaata accagctttc attttttatt
     6901 tttctgcatg ctggaattta tgacggcatg cgtagatcgt aaatggaata actaagacga
     6961 taagataaga aacaatcaga atcgtttcat aaatcccatg ctgtccagct gcgatactag
     7021 caggtggaac aaatgagata caaaaagcaa aaagtgaagt caaaagacct aaacaagcaa
     7081 cgattacttt tacaactttt ccgcctggaa cctgataggc tctttcaaga ctgtcatctt
     7141 taaaaaccag cttcaaatag cccgcgaaaa acataaagta caacatcagg tagattacaa
     7201 cagtcaatga catcgctgca agaaatgaaa gattattccc cccgccgctt aatgtaaaga
     7261 cggctgccca gattgttgca acgatcccat ttgcgatcat caaatgagca ggaacaccgt
     7321 gtgtgttttc ttttgcaaaa tattcaggaa gtaatccttc ttctgctgct acgtgaagtg
     7381 cttttgaagg agaggttacc caagaagcaa tttctccgat gacacctaaa caaacaagaa
     7441 tcgcaagaat tttcgcaatc catgcaagat ttggatcaat gtgtcttaat aggaattcta
     7501 acgattgaat cacccctgta ttaagagaca aaccatgttg aggtactgta gtagcgacgg
     7561 tcaatccgcc aaaagtatct aaaatgattg ccagaataac caatacaaac attgctaatg
     7621 gataatcctt tttagggttt tccatttcat ttgcatgact ggcagatgtt tctacgccca
     7681 tatatgctaa aataaaggaa acaaaaacaa ccagcgtagg caatttcgaa aaatcaggaa
     7741 tcaatgtttt catagacagt atcgtttgta gtggagcgcc tgaagcgaca taatggatcc
     7801 ctaagccaaa caagactaat gctggcaaca agataccagc tacaaaaccg atccgagcaa
     7861 tcaatgaagt gtatttggtt ccttttaact gtgagagtaa aatagcccag aaaataatca
     7921 ataggattgc caatttcaga tatttattgt tttgtatagc accaataccc gttgcatagg
     7981 ataatgcacc agttaagaaa tacagcatgg tcaagtagcc aactgttatc tcaaaccatt
     8041 ggaaaaagat tgcggcaaaa ccgaatcgtt ttcccaatgt gcgactgacc caagtataga
     8101 caccgccttt ttcccatcct ttcaccgttg ccatctctgc tgcgcacaat gccacaggga
     8161 taaaccatag caaaccgcct aataacaaga aaaatactaa gctgaagcct gaggtcgcga
     8221 atgtgggata ttcatataat gaaacgacca ttgagatggt catagccaaa aagccaaaga
     8281 acgttaattt ttttgcagga attttcgttt tgctttgttg catgaatctt cctcttttct
     8341 atttgaattt gtttcaattg agtttgttgc caaagaagaa aaagtccaat ccttcttctt
     8401 gttcttcttt tgtagtagtg atcgttgtaa agtgcatcgc cgattttaaa atcactgttg
     8461 tcagcacata ggcatacacg gcagtaatga tgacaccaat cacttgaatc cctaagaagt
     8521 accagctgct tggattcgtt agtaaatggc tgttagatgc aaagatccct atcagaatcg
     8581 tacctgtgaa tccgcccatg ccatgaacac cccacacatc aagtgcatca tcccattgtt
     8641 tctttttacg aaaatcaaca gcagcatgac agacgatacc cgcaatgatc ccaatcagca
     8701 tggcactttt tgcttctaca taaccggcac aaggcgtaat ggctgccaat ccagcaacgg
     8761 atcctgttag tacgtcgaca aaatcaaagc catttccttt tacttttgca atgatcagcc
     8821 aagtaatcat cgcaaatgca agagcgatga atgttgaacc aaatgcattg actgcttgtt
     8881 catctgctcg aagtgcacca ccggaattaa aaccgaacca gccaaaccaa agcagtcctg
     8941 taccgatagc agctgccata agactgctat gctgacttct ttctcctaaa ggaatctttc
     9001 tttgaccaag tgtgaggata catgctaaag agccgaatcc agctgtcgtg tggatgactg
     9061 ctccaccagc gaaatctcta aatcccatct gatccaaaaa tccgccaccc catatccagt
     9121 ggcaaacagg gaaataaatc agcagattcc aaaaaataac aagtataata tagcctttta
     9181 aattcaatct ccctgcaaaa gctcctgtca tcaaaggaac ggtaatcacg ataaacatca
     9241 gttggtaaag aaagaacatt aaaaatggaa tcgttgcacc atgaaacatg ttggggaagc
     9301 ttgtaacatg tctcatcaaa aagaaatctg ccgggtttcc aataacaccg ccgatatccc
     9361 gtccaaaggc caatccgaat ccaccaaata cccacataat cgttgttatc ccaacggaga
     9421 gaaaagattg agacatgatc gttagtatat tattcttccg tgctaatccg ccgtaaaaaa
     9481 aagctagacc aggagtcatc aggcaaacca ttgctgtaca taaaatcatg aatgctgtat
     9541 ctcctgtatt gataggtaat gtattcattt ttttcgcctc ctttctacga ttacgagtat
     9601 aaaagaaggt gtagttgttt ttttcttctg tttttttgaa ttaatacttg catttataaa
     9661 agacagacaa ttaataaaag cttgatgtgt tgtgctcttt ttcgtttttc acacaaataa
     9721 agacaaaaaa taaacggtct tctttttata gaaaaccgtt tattttcgtc tttttttatt
     9781 tttaaagata ttttttcata aggaataaag aatacttcat ataaaatata taaaaaagaa
     9841 ccgcagcgag tgtcgctacg gttctcaaat ttgttcttca tttcaacata agtttttctt
     9901 caatttgctt ataatttacg tgcaaagaat gaaacgacta atactaaaat gattgcaccg
     9961 atcaatgatg gaatgatagc cattcctgca attgttggtc cccaagttcc tagtaaagct
    10021 tgtccaattg ctgaacctac taaaccagca atgatgtttg cgatgatccc cattgatgat
    10081 cctttattcg tgattgctcc tgcaattgct ccaatgatac cgccgacgat taatgcccaa
    10141 ataaatccca ttttaaattc ctcctaactg acttcttact tcttattgta cacgtggttc
    10201 gttattttct ttgacttttt gtgcaccttt gcctacagca gattttgctt tatctgtttg
    10261 gcgtgaagca aattgtcctg tggattttgc tgcttctgtt actttgtctt gtactgtttc
    10321 ttgatctttt tggtattctt cttgtgtttt gatgtctgca acattcacat tcacttcgat
    10381 aacatctaaa tgtgtcatat tgtttacttc agtactgatc aattctttga tttggttgta
    10441 aatatcttcg atgtctttac cgtattctac gatcacatct agatccacag ctacttgttt
    10501 cttacctact tctgtattga tacctgctgt tacgttatct gtgttgacta atttttcagc
    10561 catatttgag aaaaatccgc catctaatgt caatagacca tctacatctt tcaatgcata
    10621 accgatgatt ttttggatga ctttgtcttc ataagtcaat tcaccattca tcatgcggtt
    10681 ttctcctttt tcatttgcca aaactgatcc atttgttgta ttcatgtttt tgttttccat
    10741 ctttgtttcc tcctatgatt taaaaaattg atcaactatt cctgactgtt tcaaataaaa
    10801 accgattcct gcgccaattg ctgtcatgat tatgatcaac aatgtcttga aaaatccaat
    10861 cgttaaccat aaaacagcta gcagtagtcc aagaccccca aagataatag ggagcttgta
    10921 tgctgaaaat agttctttca ttatttggcc ctcctattcc actcgagagt gcttgtagtc
    10981 tcttgtatct tcttgttctt cataaccgct gtatgtgact gccactttga ccgtttcttt
    11041 tgttccgagt actttagtta cttcttcttc gatatcttgc atcaatacac ctgttttccc
    11101 tactaatgaa gaagttcgtt tcaagtctcc gttgacatgg atatggattc gatttttcgt
    11161 tgaacgtatc cgtactttag gtgaatgaat gaactcttct tcgtgtaaat gggaacgtac
    11221 caacccttcg atcgcttttt tgtcgattgt tagtttgcca tcttcccgtt tcataacgaa
    11281 agttcctctt gactttggat agaaaagaac gactagcaag atgataatag caagaacggc
    11341 aaatactaca gctgcccaga aaatatattg ttgcatgtag taattcgtta aagtaaagaa
    11401 gcgaaatcgt tccaattgtt caggcataat atacggcgca ttgatcaacg caatcgctag
    11461 aaatactgaa agtagcagta aactcatgat aacaccgatt atttttaacc ccttattcat
    11521 tttccacctc ctataaactt atttttaccc tgtaaataac tctaccgaca atcacagtat
    11581 agatagcgaa cagttagcat agcatcagta aataaagcgc taccaaacat tgctggatca
    11641 taacggcgtt ttattgccag tctgatactg aaataacaat tgcttactta ttctctattt
    11701 acttttgatt gctggctata cttaaaggat attattcaag attaaaaatt gcaaagatta
    11761 tgctcagtta ttattacaag attctattta tt
//
LOCUS       DACDIE010000074        11709 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.074, whole genome shotgun sequence.
ACCESSION   DACDIE010000074 DACDIE010000000
VERSION     DACDIE010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11709
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.074"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            256..474
                     /locus_tag="IV250_002342"
     CDS             256..474
                     /locus_tag="IV250_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769590.1"
                     /translation="MIENRLIDQICCLDQNVDTLELIESICFNTVVLDLRAENDNCVT
                     HIIINQEMAQKLSESLKKWAEGGNNENN"
     gene            461..3151
                     /locus_tag="IV250_002343"
     CDS             461..3151
                     /locus_tag="IV250_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769591.1"
                     /translation="MKIIKKKIAGRKINWLALSLLALMFIVIFTGNVAHASIFNWFDD
                     TESQTEFLNNPVYQPYLKKYNGNFYSQFGLWIGWSVIKSMFAIVDGIQNMIPEVLDLF
                     SFVESAGLNKVYQSVLSTIVVGLMILSLVIIGYKMVTGKGTVDLKSVGINVLMSIVLI
                     LLMPTLIGSTEKLSGIMFAKTLYNDATSINNQDGGVAWSLIEQGVTDLAYINKTDQYS
                     MIDKTNDRNNLTKENFWKTDLTQVLTDNVIDKMEKENSTADNLRYELVENSDNEFVAT
                     KFSDNFLSTFVDGIKSGYYRYQANLWGISIGLIALAIAYVFSAFVIITAILELAFKRV
                     LGVLVFATDLETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNSGQGISTNIF
                     IKTIAYICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSNGFNRA
                     KGSGNGNGSDSGEGEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMAS
                     ERAIKPFKSMRDMANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSMPT
                     RLSDRKDGAKVENADRIMSSSERMREAMKNNAESNNNPVSAIQQKVQQDIEERKNAMH
                     GQAKSAEELINQKRQGAKYTPEAMNREEMLRKRVEGRTGANMDEKEALTKERIQEAKN
                     SNTGLEKLVKENVQNSTSSQGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNGQGT
                     KTIDVRENLQSSSTSQPKTVDVRENVQNSTSSQGFKSVDVRENVQSSSASQPKTVDVR
                     ENVQSSTSSQGFKTVDVRENAQKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDS
                     EIRENDKGSKPRRRFTYEDNELFRDTLNDPNPLFDSLLKK"
     gene            3165..3350
                     /locus_tag="IV250_002344"
     CDS             3165..3350
                     /locus_tag="IV250_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769592.1"
                     /translation="MQLILQISTHDFLELDELCESVKKATNEKITVTNYIELMIQEEL
                     KMKNELMNELSHIQKGA"
     gene            3354..3692
                     /locus_tag="IV250_002345"
     CDS             3354..3692
                     /locus_tag="IV250_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769593.1"
                     /translation="MARKKQKSIADLKKELAELERKLYPELGKFAYEKTGISSVKEFE
                     ETFEINQKGTREKWQKAVNFANSEFTLVHEFLASDQLLETKFQQFLRRKGETAYRIFY
                     REETQEESVR"
     gene            3841..4338
                     /locus_tag="IV250_002346"
     CDS             3841..4338
                     /locus_tag="IV250_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769594.1"
                     /translation="MAIDVSKLPLEERRKRYKRNEDSRRQNRPIKFRVDDYQYQELEK
                     TADIFGLSVPMYVKKIAVDNAGKSLKYTDRQYKEINFHLLAIGRNLNQLVKTANYLRV
                     QLNKKDTEIQKEEHQSVYLSFTTKQERLKRLYAKKNDLMIQIKSISDPLNRLEKELNK
                     LWARL"
     gene            4323..5660
                     /locus_tag="IV250_002347"
     CDS             4323..5660
                     /locus_tag="IV250_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP8769595.1"
                     /translation="MGSIIKIASKSRSIHNTISYMLNKQEDSELVTGIGVSNDLNAIE
                     DFKRTNEIFGDKGKIKLWHFTQSFDPLEDISAEKAHELGVKWAEKMFPNQSILVVTHT
                     DKGHVHNHFGISPNDQRTGKKMDIRKNDIRVLQAWNDNILIDNGISPLDMYKYNHLVA
                     DRENWKEREFRTENKISNREKIKLAVSEVAQYAKNYDDFHAELKKKHSVETYRFAKNQ
                     RIGYKLLDSEGGLILKIGGKRLGQNYDMNYLDDLFTENKKAEQIQSEEVLTEIAIRHL
                     GKSLNGRADSRINWQERAQRFELDNENIPFLRSSKDYEEFYDKYKFEIEDRIEIVLDV
                     FGENKDQFFGFAVEEEPLKYKKKCVDLVYKKVVYDISESLSTDYDQFNVHDTKPALER
                     NFKKRYIQETKRVLYLFNRAIDDGTIEKARREYEETAREIDYQKRQNQYDMDF"
     gene            5682..5996
                     /locus_tag="IV250_002348"
     CDS             5682..5996
                     /locus_tag="IV250_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769596.1"
                     /translation="MNYPYFKVSASEETKEIFNNFYNQNKGVFGSKANMFRVMVSNLP
                     VLASPSNNKFNDSESIKFEQKISELESMISNEVIEKLDDIDQKLSYSLKNKYKTEEKK
                     DV"
     gene            5989..6483
                     /locus_tag="IV250_002349"
     CDS             5989..6483
                     /locus_tag="IV250_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769597.1"
                     /translation="MFKVYFPFQWLVKGILLVLLGLILFGSYLTIKARHFPEEKPIKI
                     VTIDLKIGETYQADGVELTFKNIEVTEKRNEFAQHDRVLQVTYTLKNDTEKPIRPQQW
                     SIENKDGEELYEYAMILDNPEYIESDKAETINYYYELPNEMQDYQIVLRRANGYLVGL
                     PFTL"
     gene            6560..6874
                     /locus_tag="IV250_002350"
     CDS             6560..6874
                     /locus_tag="IV250_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769598.1"
                     /translation="MAEENKVVPFETLVINKEEFEQLFLNFFIQYDRIYDFSSSLDLF
                     ELEAEIIENSREKNGKIIISVEKFNELFSETFEIDPDYYQPQEVFREFRNQIIRSSNI
                     EN"
     gene            7138..8187
                     /locus_tag="IV250_002351"
     CDS             7138..8187
                     /locus_tag="IV250_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HAP8769599.1"
                     /translation="MDKIFKIFVSSTFTDLEDVRLQAMQGVLKAGHMPIMMEGFAATA
                     TQKEIIKKKITSCDAYMLIVGSKYGTIDPESGLSYTEWEYDYALDNGIPIYTMILEEK
                     YIQERMANGLLKLSDIETTSENYKAFVSKASSRQVDFINSIEQVKSMTEAGIMQLVKD
                     FGSSMIGWVSATIVDNVKMLENELDNLRKTRSELQVKLTNAETREIRESELPPNSSFD
                     GLLSKMFAPNSTDPILEQGMIYIMEFIREKVEQLKATIESHRGNIALGYDANKKDQVL
                     LRLGKDCLFFLADYNKAIINVRFAIQDPYFSENIDTLHVKNDVLYSEKYSEPLSLDYL
                     NKTLSQYHEIKKQQN"
     gene            8550..9386
                     /locus_tag="IV250_002352"
     CDS             8550..9386
                     /locus_tag="IV250_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAP8769600.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQAIVIVVLLINLYNAFVILQTLEGSGFKQVKITEKSF
                     FK"
     gene            9450..9881
                     /locus_tag="IV250_002353"
     CDS             9450..9881
                     /locus_tag="IV250_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8769601.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(9943..11265)
                     /locus_tag="IV250_002354"
     CDS             complement(9943..11265)
                     /locus_tag="IV250_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAP8769602.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(11419..>11709)
                     /locus_tag="IV250_002355"
     CDS             complement(11419..>11709)
                     /locus_tag="IV250_002355"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="malic enzyme; oxaloacetate-decarboxylating;
                     NAD-dependent; catalyzes the formation of pyruvate form
                     malate; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAP8769603.1"
                     /translation="LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAIL
                     PPVAKITEFSQTIAETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
BASE COUNT         4226 a         1729 c         2351 g         3403 t
ORIGIN      
        1 taattcaatc aatatactct gaaaagagca aaacatacag gataaataga gaagaaaaat
       61 ctgcaaatga agtgatagaa aaagtagaac aactcaatca atttgatatt gaatctgcta
      121 ttgattcatt taacgcaatt gatcatgaga tacacaatct caatctttac tggattacaa
      181 ctttatataa ctgcaaaaat actgtaagtt ccagagcata taataacttt gttactcaaa
      241 caagagaggt gttatatgat tgaaaatcgt ctaatagacc aaatctgctg tctcgatcaa
      301 aatgtagata ctctcgaatt aatcgagagt atctgcttta acaccgtggt tctcgattta
      361 agagctgaaa atgacaattg tgtaactcat atcataatta atcaagaaat ggctcagaag
      421 ctttctgaga gcctaaaaaa atgggctgag ggaggtaaca atgaaaataa ttaaaaaaaa
      481 gattgctggt aggaaaataa actggttagc tctgagctta cttgctttaa tgtttatcgt
      541 tattttcacc ggtaatgttg ctcatgcaag catttttaat tggttcgatg atacggaaag
      601 tcaaactgaa tttttgaata atcctgtcta tcaaccgtat ttaaaaaaat ataacggaaa
      661 tttttacagt caatttggat tgtggatagg gtggtctgtt ataaaatcga tgtttgcaat
      721 tgtagatggt attcaaaata tgattcctga agtgttagat ttgtttagct ttgtggaaag
      781 tgctggattg aacaaagtct atcagtcagt tttaagcact attgttgttg gcttaatgat
      841 actttcacta gtgattattg gttataaaat ggtgactgga aaaggaacgg ttgatctaaa
      901 atctgttgga atcaatgttt taatgtcgat agtattaatt cttttaatgc cgactttgat
      961 tggatcaaca gaaaaacttt caggaataat gtttgcaaag actctttata atgacgcaac
     1021 tagtattaac aatcaagatg gcggtgtggc atggtcgctg attgaacaag gggttactga
     1081 tttagcttat atcaataaaa cggatcaata ttccatgatc gataaaacga atgatcggaa
     1141 caatctaaca aaagaaaatt tttggaaaac ggatcttacg caagtattga cagataatgt
     1201 aattgataaa atggaaaaag agaactcaac agctgataac ctgcgttatg aattagttga
     1261 aaattctgat aatgaatttg ttgctactaa attttcagat aattttttgt caacctttgt
     1321 tgatggtatc aaatcaggat attatcgcta tcaagcgaat ctatggggca tttcaattgg
     1381 gctgatagct ttagcaatag cctatgtttt tagcgccttt gtgattatta ctgcaatact
     1441 cgagttagca tttaaacgtg tactcggagt tttagtattt gctactgact tagaaacagg
     1501 acagcgttca aaagttgttc tatcagatat tctacagtgc tatttgactg taggctttca
     1561 aggatttgga ctatccatgt ttgctatgtt tatcaatttt ctgaactcag gacaaggtat
     1621 ctcaacaaat atttttataa aaacaattgc ttatatctgt gccgtttttg ttttaatcaa
     1681 aggcagtgga acagtcatga gatactttgg tgttgatatt ggactcaaag aaggttatgg
     1741 gcaacttgct tctgcttttg gtatgggcgc aatgctgttt agaaaaggat caaatggatt
     1801 taatcgtgct aaaggaagcg gaaatggaaa tggatcagac agcggagaag gagaagatcg
     1861 aaaaccagaa aagaatttcg gcgaaactct atcaaaaaaa gctggcaaaa ctggaagagc
     1921 tttgggatat gcccatgaac gtggattatc aggtttagct tcagatggag ctacaatggc
     1981 tagtgaaaga gcaataaaac catttaagag tatgcgtgat atggctaatg atactaagaa
     2041 taagttcaaa gaaggtttag atgatggtac cgtgtccgct attaataaaa atagcaaacc
     2101 tatgttagct aaaaataaag aagatgaaac tggaaaatat gctgattcca tgccaacacg
     2161 tctttcggat agaaaagatg gtgctaaggt tgaaaatgcc gatagaatta tgtctagttc
     2221 tgaacgtatg cgtgaagcaa tgaaaaataa tgctgaatcg aataataatc ctgtaagtgc
     2281 aattcagcaa aaagttcaac aagatattga agaacgtaaa aatgctatgc acggtcaagc
     2341 taagtctgct gaagaattaa ttaatcagaa gcgacaggga gcgaaataca cgcctgaagc
     2401 aatgaatcgt gaggaaatgt taaggaaacg tgtagaaggg cgtacaggcg ctaatatgga
     2461 cgaaaaagaa gcgttgacca aagaaagaat ccaagaagct aaaaattcaa ataccggatt
     2521 agaaaaattg gtcaaagaaa atgtacaaaa ttctacaagt agtcaaggtg gccgatcagt
     2581 agatgtccga gaaaatattc aaggttcatt gaatggtcat gtcggacgga atgttgatgt
     2641 aagagagaat ttacaaagtt cttcaaatgg tcaaggtact aagacaattg atgttcgaga
     2701 aaatctgcaa agtagcagta caagtcaacc aaaaacagta gatgttcgag aaaatgtaca
     2761 aaattctaca agtagtcaag gctttaaatc agtggatgtc cgagagaacg tgcaaagtag
     2821 cagtgcaagt caaccaaaaa cagtagatgt ccgagaaaat gtacaaagtt ctacaagtag
     2881 tcaagggttc aaaacagtag atgttcggga gaacgctcaa aaagatagtg gagaaatgaa
     2941 agaaaaaaca caaaaaatca atatcgttga agacagaaag tcagcgaaaa aatttgattc
     3001 aaatcatgaa acagtgatta ttgatagtga aatccgtgaa aatgataagg gatcaaaacc
     3061 aagacgaaga tttacctatg aagacaacga gttattcaga gatacactta atgatcctaa
     3121 tccacttttc gatagtttac ttaaaaaata aagaggtgat tttcatgcaa ttaattctac
     3181 agattagtac acatgatttc ttagaacttg acgaattatg tgaatcagta aagaaagcaa
     3241 caaatgaaaa aatcacggtc actaattata ttgaactgat gattcaagaa gaattaaaga
     3301 tgaaaaatga gcttatgaat gaacttagtc acatacaaaa aggagcatga aaaatggcaa
     3361 ggaaaaaaca aaaatcaatt gcagatttga aaaaagaact cgccgagttg gagagaaagc
     3421 tttatcccga actgggaaag tttgcctatg aaaaaacagg aatttcatca gtaaaagagt
     3481 ttgaagaaac ttttgagatt aatcaaaaag gtactcgaga aaaatggcaa aaggcggtaa
     3541 atttcgcgaa tagcgaattt accctagtgc atgaatttct tgcaagcgat caattgttgg
     3601 aaacgaagtt ccaacaattt ttgagaagaa aaggagagac agcgtatcga attttctatc
     3661 gagaagagac gcaagaagaa agtgttaggt agctgattta tcgactatct aatctcgata
     3721 aaatgcggag catgagggtg ctcgtaggga gtttactccc tcgacaagcg gggaagggtt
     3781 ccctttcccc tcgcttgctt cttttgaact taaaaataat attggaaagg agaagtgcgc
     3841 atggcaattg atgtcagtaa actaccatta gaagaaaggc gaaagcgata taaacgtaat
     3901 gaagatagtc gacgacaaaa taggccgata aagtttcgag tagacgatta tcagtaccaa
     3961 gagctagaaa aaacagcaga catttttggt ttatctgttc caatgtacgt gaagaaaatc
     4021 gcagtagaca acgcagggaa aagtctaaaa tataccgatc gacaatataa agaaattaat
     4081 tttcatttac tagcaatagg aagaaatttg aatcaattag tgaaaacagc aaattatttg
     4141 cgtgttcagt taaataaaaa agataccgag atacaaaaag aagaacatca gagtgtttac
     4201 ttatctttta caactaagca agagcgctta aagcggctat atgctaaaaa aaatgactta
     4261 atgatacaaa taaaatcaat aagtgatcca ttgaatcgct tggaaaaaga gttgaataag
     4321 ttatgggctc gattataaaa attgcttcaa aaagtaggag tattcataat acaatttcat
     4381 atatgttaaa taaacaagaa gattctgaac tcgtgacagg aattggcgta tcgaatgatt
     4441 taaatgctat tgaggatttt aaacgaacca atgaaatttt tggtgataaa gggaaaataa
     4501 agctttggca ttttacacaa agctttgatc cactagaaga tatttccgct gaaaaagcac
     4561 atgagttggg tgttaagtgg gcagaaaaaa tgtttcctaa ccagtcaatt ttggtcgtca
     4621 cacacacaga caaagggcat gttcacaatc actttggtat ttcaccaaac gatcagcgaa
     4681 caggaaagaa aatggatatt cgaaagaatg atattcgagt tttgcaggcc tggaatgata
     4741 atatacttat tgataatgga attagcccac ttgatatgta taagtataat catttagttg
     4801 cagatcgtga aaattggaaa gaacgtgaat ttagaactga aaataagatt tcaaatcgag
     4861 aaaaaataaa actagctgtt tctgaagttg ctcagtatgc gaaaaattat gatgactttc
     4921 atgcggaatt gaagaaaaaa cattctgttg agacctatcg atttgccaaa aatcagcgta
     4981 ttgggtacaa actcttagat tctgaaggtg ggttaatctt aaagattggc ggaaagcgtt
     5041 tagggcaaaa ctatgatatg aattatcttg atgatttgtt cacagaaaat aaaaaagcgg
     5101 agcaaattca atctgaagag gtactcactg aaattgctat tcgacatctt ggtaaatcgt
     5161 taaatggtag agcggatagt agaattaatt ggcaagaacg agcgcaacgc tttgaattag
     5221 ataatgagaa tataccattt cttagatcat caaaagatta tgaagaattt tatgacaaat
     5281 ataaatttga aatagaggac agaatagaaa ttgtattaga tgtttttggt gagaataaag
     5341 accaattctt tggatttgca gtggaagagg agcctttaaa gtataagaag aaatgtgtag
     5401 atttggtgta taaaaaagtt gtttatgata tctcagagag tttatcaact gattatgacc
     5461 aatttaacgt tcatgatact aaacccgctt tagaacgtaa ttttaagaag cgttatattc
     5521 aagaaacaaa acgtgttctt tatttattta atcgtgcaat tgatgatggt acgattgaaa
     5581 aagctcggag agagtatgaa gaaacagcaa gagaaattga ttatcaaaaa cgacaaaatc
     5641 aatatgatat ggatttttga gtaaacaggg agagagaaaa aatgaactat ccatatttta
     5701 aagtctcggc ttcagaagaa actaaagaaa tattcaacaa tttttacaac caaaacaaag
     5761 gagtttttgg ttccaaggca aatatgtttc gtgttatggt ttcaaattta ccagtgttag
     5821 ctagtccatc aaataataaa tttaatgatt ctgaaagcat taaatttgaa caaaaaattt
     5881 cagaactaga atcaatgata agtaatgaag taattgaaaa gcttgatgac attgatcaaa
     5941 agttatcgta ttctctaaaa aacaaataca aaacggagga gaaaaaagat gtttaaagtt
     6001 tacttcccgt ttcagtggct cgttaaagga attcttttag tgctactagg attgattctt
     6061 tttggaagtt atctaacaat taaagccaga cattttccag aagaaaagcc aataaaaatt
     6121 gtaacaatag atttaaaaat aggtgaaact tatcaggcag atggggttga actaacattt
     6181 aaaaatattg aagtgaccga aaaaagaaat gaatttgcac aacatgatcg tgtattacaa
     6241 gtgacctata cacttaaaaa tgatacagaa aaaccaatac gtccacaaca atggtcgatt
     6301 gaaaataagg atggtgaaga gctttatgag tatgctatga tattagataa tccagagtat
     6361 attgaaagtg acaaagcaga aacaataaat tactattatg aattaccgaa tgaaatgcag
     6421 gattaccaaa tcgtattacg tagagcaaat ggttacctag ttgggttgcc atttacttta
     6481 taagttttat tttgtcagta gcttttaagg taatatcagt tttgtgattt ttgaaaatgg
     6541 aagtggggga actgggagaa tggctgagga gaataaagtt gttccgtttg aaacattagt
     6601 gataaataag gaagagtttg agcaactttt tcttaatttt tttattcaat atgacaggat
     6661 ttatgatttt agctcgtcac tagatttatt tgaattagaa gcagaaataa tagaaaacag
     6721 tcgagagaag aatggtaaaa tcattatttc agtagaaaaa ttcaatgaat tattctcaga
     6781 aacatttgaa atagatccag actattatca accacaagaa gtttttcgag agtttagaaa
     6841 tcagattatt cgttcaagta atattgaaaa ttgagtcaat aaaagaagcg ttgaagaaaa
     6901 gttccttctt tcgacgcttc tttttaaaag attttacaaa tgtcttatgg agatttgctt
     6961 caatgactac ttgtagtcat ttcgtttaaa ataattatgt tacaccctta tgtgtcacag
     7021 agaaatactg atataataag aaataaggta tgtaacaata gagtatactt atacggtaca
     7081 ttaagaaatg ttaagtataa aattaatgac tttttggtct gaaagctgag ggattcaatg
     7141 gataaaatat tcaaaatttt tgtgtcatca acatttactg atttagaaga tgttcgactt
     7201 caagcaatgc aaggggtatt aaaagcggga cacatgccta taatgatgga agggttcgct
     7261 gccacagcta cacagaagga aattataaaa aaaaaaatta ctagttgtga tgcgtatatg
     7321 cttattgttg gtagtaagta tggaacaatc gatccagaat cagggttgag ttatacagaa
     7381 tgggagtatg attatgcatt agataatggt attccaatat acacgatgat tttagaagaa
     7441 aaatatatac aggaacgtat ggcaaatggg ttacttaaat tatcggatat agagactact
     7501 tctgaaaatt acaaagcttt tgtgagtaaa gctagcagta ggcaggttga ttttattaat
     7561 tccatagagc aggtaaaaag tatgacagag gccggaatca tgcagttggt caaggacttt
     7621 ggaagctcga tgattggttg ggttagcgca acgattgttg ataatgttaa aatgttagaa
     7681 aatgaactag ataatttgag aaagacaaga agcgagttac aagtgaaatt gacaaatgca
     7741 gaaacaagag aaataagaga gtcagaatta ccgccaaata gtagttttga tggattatta
     7801 tcaaaaatgt ttgctcctaa ctccactgac cctatccttg aacaaggaat gatttatatt
     7861 atggagttta ttcgagaaaa ggttgaacag cttaaagcaa caattgagag ccatcgtggg
     7921 aatattgcat taggatacga tgctaataaa aaagatcaag tacttttgcg cttaggaaag
     7981 gattgcctat tttttttagc tgattataac aaagctatta tcaatgtacg atttgctatt
     8041 caagaccctt atttttcgga aaatattgat actctccatg ttaagaatga tgtattgtac
     8101 agtgagaaat attcagaacc actttcactg gattacctaa acaaaacgct tagtcaatat
     8161 cacgaaataa agaaacaaca gaattaaatt tcctgccaaa agcacgccac gcaatttgaa
     8221 tacgattttg aatacgatta gaaaactaat caagatgatt tatcattaat aaagcgatta
     8281 gctttaatga aaaatcattg aaatatcaac tatcttcaat tatcatcaat gaacttaact
     8341 tcatggcgga agaagaatag aacaaaagaa tcgataaggt gtaagaaaag ctggactctc
     8401 tgaaaagtag agcccggttt tttcgtttag cactaaattt ccttaaaaag tagaaaagct
     8461 ttcgaagcct cgtttgtttc ttttactgta taataaaaga aaagagtaac aatcgctaaa
     8521 agatataaac aagagaaagg aaatacaaga tgattggtct gatttatttt gtggtgatcg
     8581 ttctggcgaa cacagttggc gctatttccg ggatgggtgg aggcgtgttg atcaaaccga
     8641 ttcttgatct tatcggcgcc cattctgttg cagggatttc tttttactca acagttgcgg
     8701 tattcacgat gtccattgtt tcgactgtgc gacaagtatc aagcgggaaa tcactgaatt
     8761 ggcagattgt cggttgggta tctagtggag ctgttgttgg aggagtagtc ggcaacattg
     8821 tattcgaagt ttttttacag ttatttgaaa acgagaagca tgtgcagctg atccaaattt
     8881 ttttgacggt attgaccttg gtttttgcgt tcttctacac caagcatcat cagccaaaat
     8941 ttcatttgac gtcatggact tggtacctta tatgtggggg ggttctgggc tttttagcta
     9001 gttttttagg tattggtggt ggaccagtca atgtttcttt gttgatgctg atgtttgcat
     9061 tgccaataaa agaagcaaca ctatattctc tatctacgat tttcttttct caactggcaa
     9121 aattggtaac gattgcttta acaagtagct ttatgcgctt tgatttatct atgttgtttt
     9181 atgttatccc cgctgctgtt attggaggtt tgtggggagc aagatttagc agggttttgt
     9241 ctccaaaaaa agtgaccttt atttttcaag cgattgtcat cgtggttctt ttgatcaatc
     9301 tttacaatgc tttcgtcatc ttgcaaacat tagaaggatc tggattcaag caagtcaaaa
     9361 tcactgaaaa gtcttttttt aagtgaaaaa aggagtatac tgtgataaaa aaattcattt
     9421 aaaaaaataa aaattggctg gtgatttgga tgttgcggga aatgcgttta ggagatgcag
     9481 aagcactacg agaaatcaat gcggaacaat taggatatga tgtaccatta accgttacgg
     9541 tgagacaaat gaagaaactg ttgaaggagc cagaaaaaaa ttttttcttg gtctatgaag
     9601 atcctacttc taaaaaagca gctggctatg tacaagcgga gtcctatgaa tcgattttca
     9661 gcgatccgat gttcaatatt atggcacttg ctgtgtctaa acatgctgaa aaaagaggaa
     9721 tcggaaaggc cttgatgact ggcgtagaag aagcgaaaag acgaggaata tcagcaataa
     9781 gacttaattc tgcagaatac agagtagaag cacaccgatt ttatgaacgc atcggttatc
     9841 attccgataa aatgcaaaaa agatttttga aaatcctgta aatttttatc aaaaaatacc
     9901 tggtcatcaa gccgatactt gatgatcagg tatttttttt gactaagaaa gtaattgcat
     9961 caagaaacct gagatgatca aaacgatggc accaccaagc cgattgccca tttgagcaaa
    10021 agcaataagt tccatccgat tagctgcaga taagacagct acatttcctg taccacccat
    10081 cgagttgttg cataagccag cagtaatagt tgattcgatt ggatataaac caaacaaacg
    10141 accgacaaat cctgaaacta ctgaaatcgc gataacactt gtcaaacata agacaacaaa
    10201 ttgccatgtc aaagcagaag ccaatacatt tagattcaat aacgcaatac cgataccggc
    10261 aagaacagct gctgtcaagt ttttgacaat caagttgttg aacatgatag ctgcatcttc
    10321 gtaatattct ggtaataggt ttgcgatttt acagataaca acgataatga tcatgaatgc
    10381 gtaagcatgg actttaggaa caaaatagtt acagatttgt cctaagatga agaaagtcaa
    10441 tgataccatc aaaccgacac ctaattgtgg aatggaaggt tttacatttc taggttttcc
    10501 atcgccaagg tcgccttttt ccattaattt accattaccg ttgaattttg gcattgattg
    10561 tcccgcacga gcaactaacg ctgcaccaat gatagctagg acatttgtca ttgctgtcgc
    10621 aggaatcagg cgggaaatga tagaagaagc gtccgtacct aaaacatttg cataaatcgt
    10681 tgataaaggt acagctccgg ctccaacacc accagccatt gcgggcaatg agatatataa
    10741 aacggaattt gcaaaaccat ttccgataag catacctacg agtcctacgg caaaaaagga
    10801 gacgaccatt gaaataagtg caacgggaat aaaacgaaca gaagctttca tcaatagatt
    10861 acggttcata ccaaggatgc taccagtaat caaggcagca atataaaagt ctaaaaagcc
    10921 catattgctg acaaagtttt ctgtggattt tactacatac tcaggtaaga tcccaaatgt
    10981 agcaatcgcg gcagaggcaa agatcgcaaa aacggagcca ccacctaaat aacttttgac
    11041 tatagggatt tttgttccga taaaatgaaa taaattaccg aaaatgacca aaacagcgat
    11101 tgggccaatc atattcgtcg gtaattttcc taaagccatt gcaatgatca ggacaaggac
    11161 catgatcaaa taaaggggca aacttacgcc ggtaattttt gttgaccaaa acccttcttt
    11221 tttcttttcc tcaactgcag ttgcggttgt tgttttttgt gccattttta cagatccttt
    11281 cttattctgt atcgtatgaa aaaagccaaa gagaagcggc aaaagagcag tagttggctg
    11341 gctgctgttt tgccgcagtc gcttttttct ttttagacgt catacaaagt ttctgtatgt
    11401 tcttttacta taacaatctt aatcttctaa agatttatat tcaggaaccc atttagcaga
    11461 ttcaacagct tctttgatat ccgtgatttc ttcacgattc aatttttgtg caacaactga
    11521 ttttgcgaca gtttcagcaa tcgtttgaga gaattcagtg atttttgcta ctggaggcaa
    11581 gatagctgct cctggttttg ttacatcaac gattccgcct aatgctcgac tagcttgaga
    11641 aatgatttca gcatttactc gtgtagctgt tgaagcaatc aaaccaagcc ctaaacctgg
    11701 gtacatcaa
//
LOCUS       DACDIE010000075        11681 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.075, whole genome shotgun sequence.
ACCESSION   DACDIE010000075 DACDIE010000000
VERSION     DACDIE010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11681
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.075"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(175..2250)
                     /locus_tag="IV250_002356"
     CDS             complement(175..2250)
                     /locus_tag="IV250_002356"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAP8769604.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEIIPFEILDVKTDRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2252..3169)
                     /gene="glyQ"
                     /locus_tag="IV250_002357"
     CDS             complement(2252..3169)
                     /gene="glyQ"
                     /locus_tag="IV250_002357"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAP8769605.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            complement(3553..4365)
                     /gene="recO"
                     /locus_tag="IV250_002358"
     CDS             complement(3553..4365)
                     /gene="recO"
                     /locus_tag="IV250_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAP8769606.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            complement(4508..5407)
                     /locus_tag="IV250_002359"
     CDS             complement(4508..5407)
                     /locus_tag="IV250_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAP8769607.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5422..5823)
                     /locus_tag="IV250_002360"
     CDS             complement(5422..5823)
                     /locus_tag="IV250_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HAP8769608.1"
                     /translation="MPMDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(5801..6277)
                     /gene="ybeY"
                     /locus_tag="IV250_002361"
     CDS             complement(5801..6277)
                     /gene="ybeY"
                     /locus_tag="IV250_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAP8769609.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6294..8486)
                     /locus_tag="IV250_002362"
     CDS             complement(6294..8486)
                     /locus_tag="IV250_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAP8769610.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            complement(8510..9481)
                     /locus_tag="IV250_002363"
     CDS             complement(8510..9481)
                     /locus_tag="IV250_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAP8769611.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10186..11565
                     /gene="brnQ"
                     /locus_tag="IV250_002364"
     CDS             10186..11565
                     /gene="brnQ"
                     /locus_tag="IV250_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP8769612.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHYVPFAALGLGWVVPTLLGYAGSLLFYYVYRVSGYK
                     QEASELPEE"
BASE COUNT         3166 a         2440 c         2128 g         3947 t
ORIGIN      
        1 tcgattttat ttttatttcc ttaattttca taggtatttt caaaagttac acaaaaaata
       61 aaataacaaa aaaaattaac attgaattaa taatagttat ttatatttat aacaaataaa
      121 ccagagaaac agcctatttt gtgcctgttc tctggtttat ttacatatta gaaattattt
      181 tgtgatgatt tgatctaaac ttgctagtga caaagccatg gcagcaattt gacttaattg
      241 cgttaatcgg ttcttacgta ctgctggttc ttctgccatg accatcgtct gatcaaaata
      301 attttcgatc agtgggcgaa ggcttacaag ctgatcataa ttttcgttga tcgtttggtc
      361 agcaaagctt gttttcattg cctctactgc tttgtgcagt tctttctccg cttcgttttc
      421 gaacagttca gggtctactt ctgattgttt ttcaagctcc gcttttttcg ctaagttcac
      481 gacacgtgtc aaagcttcca tcgatggttt gaagtcttca tctgcaagat gctgcttgaa
      541 caattgtgct gctgcaaata cttttgtcag atctttttgt tctgcgttca aaacagcttc
      601 gataacatca tgacggatat tcttcgttgt caataattgg cgcagacggc ctttgataaa
      661 gtcgatcacc tgttgttgtc ccgctgacaa gccaatgcca aaacgatccg tatctttact
      721 gataacttca ctaatcgttt cttgcagaac agacaaagga aaggcccatt ttttcgattc
      781 gatgattcga acgacaccat atgcttgacg gcgcaaagca tatggatcgt ttgagccact
      841 tgggatcatc ccaactgcaa agaaagaaaa gatactatct aatttatcag caatcgctaa
      901 aacagaacct actgtagtct caggtagatc tccttcactg gatataggca tgtaatgttc
      961 acggattgct gttgctacgg ctggtttctc gcctttaagc agagcatatt tttctcccat
     1021 aatcccttgt agttcaggga attctcctac catgttggtt actagatcaa atttatagat
     1081 ttcagcagcg cgttgcagat ccatcaattc ttcctcagat agtcctactt gttcgcctat
     1141 gatgcgagcg atgacttgta cccgttgcat tttctcatag atgctaccga ttttttcatg
     1201 gaaagtaaca gtttttaatt tttcaacgta ttggtcgatt gaagctttct tgtcttcatt
     1261 ataaaagaat tccgcatctt ctaaacgagc agtcaatact ttttgattgc ctcgtaccac
     1321 attttccaat tttacccgat cgccattacg agcagaaata aaatgaggaa gcaacatacc
     1381 ttgatcgttc cgtacttcga aataacgttg atgttctttc atagaagtga ctagtacttc
     1441 ttcaggaaca gaaagatatt tttcctcgaa gtctccagaa aagacagttg gatattcgac
     1501 tagattgttg acttcttcta gtaaatcttc atctagatca atgatccagt gatttttttc
     1561 tgctaattcg tttgcttgtg taacgatcat ttcttgtctt ttttgcggtg aagcaataac
     1621 aaattgttca tttaattttt cttcgtattc ttttgcatgc gcaaatgtta catcttcgcc
     1681 taagaaacga tgacctcttg aagctctgtc tgttttgaca tccaagatct caaacgggat
     1741 tatttcgtca tctaacaatg ccacgatcca gtgaataggg cggatatatt caaaatcgta
     1801 ttctgcccaa tgcatcgtta ctgggaaagt gaggctttta atgacatctg ctaaacctgt
     1861 caatacttct tttgcttctt tgccgtgagt gtatttcgtt acatagacat attccacgcc
     1921 ttttaattct ctaaaagtga tagcatctgt cgtcagacct tgtccgcgga caaatccttc
     1981 agctgctttc gtccagtttc catctgcgtc caaagcaatt tttttggctg gtcctttgac
     2041 ttcttcttcg ctgtccgctt gtcgttctgg aatatttgtt actttgattg ctaaacggcg
     2101 cggtgtggaa aatgtttcga tcgtatcata agtcaattgg ttttcatcta aaaattttgc
     2161 tgttttttct tctaattgtt tcatgcttgg tgtgactaca tgagcaggca tttcttccag
     2221 cccgatttca agtaataagt cttttgccat cttattcagc ctccttcacg ttttctggat
     2281 ttgctgtgtt tttattcaat aaagggaatc ctaatttttc gcgttctgcc acaaagattt
     2341 tggctacagc acgagccatg ttgcggatac gggaaagata acctgctcgt tctgttacag
     2401 atactgcacc gcgagcatct aataaattga atgtatggct gcatttcaaa atataatcat
     2461 acgctggatg aacaagattt tcatcgatac agcgtttagc ttctttttca aaacggtcaa
     2521 aattgcttag tagtaagtct tggtcgctta cttcaaaaga atatttggaa tgttcatatt
     2581 ctggttgttt gaagatctcg ccatatttca cgccatcagc ccattctaaa tcatagacac
     2641 tttcaacttc ttggatataa gaagcaagac gttctaatcc ataagtgatc tcagaagtaa
     2701 ctggtttaca aggtagtcct ccgacttgct ggaagtacgt gaattgtgtg atctccatcc
     2761 catcaagcca tacttcccag cccacaccgg cacaacccat cgatgggttc tcccagttgt
     2821 cttcaacaaa acgaatatcg tgttctaatg gatcaattcc taaaaggcgc aagctttcta
     2881 aataaagctc ttggatattt tcaggtgaag gtttcattac gacttgaaat tgatgatgct
     2941 gatacaaacg gtttgggttt tctccataac gcccgtcagc tggtcggcgg gatggctcta
     3001 cataagcagc attccatggt tccggtccga ttgcccgtaa gaatgtatat gggctcatcg
     3061 tacctgctcc tttttctgta tcataagctt gcatcagcat gcagccgttt gatgaccaaa
     3121 atttttgtaa tgttaaaatc atatcttgga cggtcaattt tttactcatc tgttttgccc
     3181 ctttcaattt ttaagcgttg ctgtcaataa gaatacagga aagaaaacga caaaaagtcc
     3241 ctatgtctga ttacagacat agggacgaat aatttcgcgg ttccacccta cttccgaata
     3301 aagattcggc agcttcattt atcgtactcg caagtgcctc ttcaaaaatc gtttgggtcc
     3361 ggcttccact ctcccgaact cgctaaacca cgtgtttttt actgatcttg gtcaatgtac
     3421 gtattcattt tgcagttttc ttgtttgtat aatagatcct ctcagtgaac gatctattta
     3481 atctacggtt aataatagac ggaagtccct gtatttgtca aatagttttg atttttttgt
     3541 tgtatctttc atttattctt ctttttcagg tatctctttt tcaggcatat ttttctctgg
     3601 cgtctttaat acgtgtgccc aagacttcat ttgatcaatg aactttttgc tttttagatg
     3661 aatacctaca tattcatcat acaactggtc aatgaggtca cggattgcct gcttcgtttc
     3721 ttctttcaag tggattccac tgatcttatc ataagaaata gccgaaaaca ttcgaacgaa
     3781 ataaacagcg cgaggatctg catgataacg gtgttcgtcc attggccagt gtttctcgca
     3841 tatcacaccg ccatatttgg aagaatagtc aaattttcct tttgtttccc cgcatacgac
     3901 acagtgcgtc caaattggcg cgattccgaa gcgttgcagt atttgaactt caaagatatt
     3961 cgtgatgatt tctgcatcat ctccttggtc catccgctgc agagcttgtt gcgtaaattg
     4021 gaataaatgc gggtcatatt gatgatcttc gattgctgca tctactagat ttagcaaata
     4081 cgtaccataa ccgctcaaaa agatatcttc ttggatgttc cgaaacgcat ggacgtcttt
     4141 gacactgctt aaaaacgaca aaccttcact tcgaaaatta ccgatataga ctgcttcggt
     4201 aaaggggagc aaagccggag ctaatggctg attttgccga tggattcctt tgatataaaa
     4261 catcattttg ccagaagatt ccgtaaagat tttgactaat ttatcttttt ctttgtaatt
     4321 acggctagat aaaatcaagc ctttcgattc tgcaagctgg ctcatattct ccctcctaaa
     4381 aaataaatct ggaaaagcag attctgccga tccagattat ttatgttacg atcttccgct
     4441 gtttttctca acgtacggat ttttatccag tttatttcta tacctaacct ataagaagtc
     4501 cagaatatca gtactcgtct tttcggtagc cgaagtcttg cagatagact tttttgtctc
     4561 gccagtcttt ttggactttg acccaaagtt ctagaaatac tttgtctcct aaaagatttt
     4621 cgatatcctt tcttgctttc gttcctactt gcttcagcat ttttccgcct ttgccgataa
     4681 tgattccttt ttggctatct ctttcgacga tgatcgtggc ctggatatgg attttatcat
     4741 tctcgtttcg cttcatcgaa tcaacaacga ctgcgacaga atgaggcact tcgtctcgtg
     4801 ttaaaaacaa gactttctca cggatcaatt cagaaacgat gaagtattca ggatgatcag
     4861 tcacttgatc atctggaaaa tattgtgggc cctctggcat ttcactgact aatacatcca
     4921 ttaagcgttc gacattgttt ccttctgtag cagagatcgg aatcacttgt gcaaaaggca
     4981 tctgcgtcga ataatcttcg atgattccca gcaactcgtc tggatggatc ttatcgattt
     5041 tattgataat cagataaact ggagaatgca cgttttttag acgttcaata ataaaatcgt
     5101 ctccccggcc gcgtttttga tcagcactga tcataaataa gactgcatcc acttctttca
     5161 acgcactgta agctgcttct accataaaat ctcctagacg atgctttggt ttatggattc
     5221 ctggtgtatc gataaaaatc agctgagctt ctggtgtagt atatactcct tggattttat
     5281 ttctagttgt ctgtgccttg tcactcataa tggcaatttt ttgacccacg attcggttca
     5341 ataatgtgga ttttcctaca ttcgggcggc cgacgattgc gacaaaccct gatttatgtt
     5401 ctggcatata cgttctcctt tttaattaaa caacaaatga tatattttag ggacaaataa
     5461 aatcaaccca atgatgacag caaaacaagc ggtcactaaa actgctccag ctgccatatc
     5521 tttgattttt ttcccaatcg gatgaaaatg aaaatccgta aacatgtcca ctacattttc
     5581 aaaaacagta ttgatgatct ctactatcca aacgagaaaa acagctaaaa gcagccataa
     5641 ccactctatc cgatcaagct ggaaaatcac gcccataaat aaagcaagga taccgaacgc
     5701 aacatgtttc ctcatattgc gttcttcctc aaaaacggtt ttgactcctt ggacagcaaa
     5761 ttccacggac atcaggaaat gtttgttttt ctctacgttt ttatcttttg agtccatagg
     5821 catccaagat ctctttctgt aaaccaaaca tttctttctc atcttctggt gtcatatggt
     5881 catacccgtt gatatgcaag aatccatgta ccgctaaaaa gccaagttcc cgatcatatg
     5941 aatggccgta agcttctgct tgttcagcag cacgttcaat agagatcatg atgtctccta
     6001 gtacacgagg caatggattc tctccatctt caaaaatcac tgggatttcg tcttcgcctt
     6061 cttcttccag agcaaaactg atgacgtctg tcggtgcatc ttttccacgg taatcacgat
     6121 tgattacttg gattgccgca ttgtccatga aagtaacaga catttctgtt tcctctggca
     6181 gctttaaata agatgctgca aattgcagca agtcatcgat ttctttcatt ttctcttctg
     6241 agacttggtt tgtttcatcc ataaaggtaa tatccattat acatcgcttc tttctattct
     6301 tcttgttctt cctttatctt ctcaacttct gttttcattt taggatattt gatccgagaa
     6361 tggaaggtgc tacttaaacc gctgatcaat gatttttcca cttttcggat ttctttcaag
     6421 gtcagtccac tatcgtctaa ttgaccatcg gaaatacgct cttcgataag attatggaca
     6481 aaactttcga ttttttcgct cgtcggatga tccattgccc gtactgcagc ctcacaactg
     6541 tcagcaatat tgacgatccc tgcttcacga ctttgcggtt ttggtccagg ataacggaat
     6601 tgctcttctg ttacttccgg attgcgttct ttcgctttga tgtagaaata acgcattagc
     6661 gtggttccat gatgctgttt gcaaatatcg atcaccattt gcggcatatt ataatcttcc
     6721 aatattttgg ctccatctgt cacatgtcca aagataatat gtttactgtc ttctggcaat
     6781 aagaaattgt gcgggttttc cgctcctgaa ggcaagttct caacgaaaaa gctagcatgt
     6841 ttgatcttgc cgatatcatg ataatagcaa gctactcgag ttagcaaaga acgtccgccg
     6901 atttctgcta cagcgttagc acttagatta gctaccatca tactatgatg ataagtacct
     6961 ggtgcttctt ccagcaactg tttcaataaa ggatgatttg gattgctgag ttcatttaat
     7021 acaattacac tgtcatcatt gaccatcaat tcaatatatg ggtgtagacc gatcgtcagc
     7081 aagaatgaga agagtgcacc agtgaacccg cagacgagca ttaaccaagt cgttgcatca
     7141 ccaaagctca ttccttgata gacgatcaaa atgacatcca tcatcacagg aaaaataatg
     7201 acccacatgg cagcagataa tccttgttct gagatccgtt tgcgtctgac aatcgttgcc
     7261 atgagcccag agaaaacata agacatcaag ataatcgtta gaaagttcgt gccaatcgaa
     7321 tcataaaaaa tgaaaagtgc ggatactgct tggaaaatac cagccatgat ccctgctctt
     7381 cgattcaaga aaaagcttaa aacaagtggt gcaaaagcag cgggataaaa cagcgggata
     7441 taagtagctt gttccgtttg gaataattgg aaaaatttca tcagcactat gctgactgac
     7501 atagctgtca catagaaaag cacgtatttt gttctttctc ctgcattttg gaattgaagt
     7561 gaattataaa tcagtacgcc gatttgaagc aaaacagcta aaaccatggc aattagcgga
     7621 aaaatcgaag ttgtttgatt agtcagtcct agtaattcca attttttcat agctgaagca
     7681 tcgatttgat taccttcacg aacaatgatt tctccttgga agatcatcac tggttgtaca
     7741 gcttctcgtg cggattgttt caattcttct gtctttttct cattcaatac gtcattcact
     7801 acgatacctt gatccactaa gtagcgaatc atttgttgtt gtgctggtgt gacattcaaa
     7861 tattggattt gctcttctgc ttcttgttta aaagcttcca attcactttc acgaacttgt
     7921 ttgctcatct gttcgtcaat gagcttcaga ctctcatccc tcacagtatg gatctcagtt
     7981 tcagtcattt caaatatcgt cgaataaaaa ttgttcggta gtttttgata aaacgcaaca
     8041 ttttctgcat tcacattttc aaaatttttc ttcagcgcgg cgatccgctc ttctgtagta
     8101 ggctttggga tattttcatc ttcctttgcc ttatcaatct tttcgtcata agatttgttg
     8161 atggcatcat tcgtcttatc gatcaattca aatagttgct tgatccgatt gttctggtca
     8221 tctgcaagat ctttttggta agtgtattct ggagtgactg cttctgctgc cagttttctt
     8281 ttttgttcgg tttcttccgt gttctcgatc gttttgttcg cacggatact ttcttctgct
     8341 acttggcctt ctctgaaatc attggccttt tgtttgacac tactaaatgt aacgccgcag
     8401 ataatgataa aaaacaagaa cagcaggaaa ggcaatgctg cctttccaag tttttcttgt
     8461 agtttctgca gtgttttatt caacatgtgc ttatcctcct aagcaaagct tattcttgat
     8521 ggagatctgc cttttcataa gctttgatga tttcggcaac tacagggtgc cgaacaacgt
     8581 cacttgcttc aaaattaaca aaatcgattt tcttgatttg ctttaatgtt cgttctgcat
     8641 gaacaagacc acttgttgtc cctcgaggca gatcaatctg actcgtatct ccattgacaa
     8701 tcatttttga ttgataacca agacgtgtca ggaacatttt catctgagca actgtcgtgt
     8761 tctgtgcttc atccaaaata acgaatgcat catccaatgt ccgcccccgc atgtaagcta
     8821 aaggagcaat ttcgatcacg cctcgttcca tcaatcggtt cgtatgatct aatccaaaaa
     8881 tttggtataa agcatcataa acaggacgca gataaggatc tactttttct tttagatccc
     8941 cgggaagaaa gcctagattt tcccctgctt ccacagcagg ccgcgtcaaa atgatttttt
     9001 gcacttcacc ttttttcaag gcagcgattg ctaatactac tgcaagaaaa gtttttcctg
     9061 tcccggctgg tcctacgcca aatacgatat catgtttggc taccgattca acatatttct
     9121 tttggccgct gttttttacc cgtataggtt ttccattacg gtctttgacg atctcttcct
     9181 catacatctc aacaaaataa tctaatgtgc ctttttgccc cattttcagt gccgtcacga
     9241 catctggtgt acttacatgg attcctcgtt tgatcaattc ctgcaatgtc cgaatgactg
     9301 aggcagccaa ggaaatctgg gattgttcac caatgatctg aatgatttct ccacgtgtat
     9361 ggatggttgt ttctgttgtt tcttcaatca gttttatatg cttgtcgtgt gcacctaaaa
     9421 gcatgctgac atcatcagca gccgtcaatc taatctctaa agaactagac tcttccgtca
     9481 aaaatagaca tccccttttt ttcgattcat tctttcctat tttaccatac tcagaagaaa
     9541 ctatatagca aataagaaaa ggttctaaaa gagttgctta accataagta ccaagaaacg
     9601 ttaaaaaata gctgcttcca ttctctaaag gtctcgtttg aataaaaaaa gctgatcatg
     9661 taagctatgc ataagataga ggacaagtat tccgtctaac ttagcaagta ttctctgggt
     9721 atttttcttg tttcaaaaaa agaaaacaaa atatttgaaa tattcttgca tttcaaaaaa
     9781 aacaggtgta ttataacgtc aacaaattaa tatacagcaa agcgtcgagg aaaacagtag
     9841 gctattcagc gatttattcc cagtgagtca tgtaagtgag aaatgacaat aaatcgaata
     9901 gacgaacatc atttccgagt caatgtcgaa aacgtagatc attgtggctg cctccattat
     9961 caggctagcg gataacttta tcgaaaagcg tccgtgaatg aagaagcttt tttaagcgaa
    10021 aaaaggtggt accgttctag aatctagagc ccttttccca agtggaaaag ggcttttttt
    10081 atattttaag gtggtggttt tcatggaatc tgatattagt ggtatttcta gtttctgcaa
    10141 cgtttatttt gttactcaaa atgataatta ttggaggaaa aatttatgga agaaacaaaa
    10201 acttattcat ggaagcagtt attgatggtc agttcactta tcttcgggat gttcttcggg
    10261 tcaggtaatc tgatctttcc tgtccactta ggtcaaatgg ctggatcaaa ctggatcagc
    10321 gctggtgtag gatttgccat ctcaggcgct cttttcccat tattagctat cttagctgta
    10381 gtagtgacaa aaagtgatgg tctgtatgac ttagctaaac ctgtcggaaa atggtatgct
    10441 gccttgttct tgatccttgt tcatttaacg atcggaccat tctttggtac accgcggaca
    10501 gcagcaactg cttttgaaat ggctgctaaa ccctttttgc ctgaaaaata tgattcatta
    10561 ggtatgtttt tattctctgc tcttttcttt ttacttgctt atttccttac tgtacatcca
    10621 acacgtttgg tcaaatatgt cggaaagtat ttaaactcct tgtttcttgc attattagct
    10681 atcatttttg tcgtttcttt attccaaccc atggggaatc taaaccaatc tgctgcagat
    10741 tcttatcaga cgaacgcttt gacaaatggc gttttagaag gatacaacac ggttgatgca
    10801 gtagctttac ttgctttaag tgtcactttt gtccatgcag ttcgtgatct tggttttaaa
    10861 gataaaaaag tcacagaatt aacagcaaaa gctggtagcc tgagcgttgc gttagaagtc
    10921 atgatttatt ttggtctggt ccttctcggt gcgatgagtt tgaataagat gtctttatca
    10981 gaaaatggcg gaactgcact ttctcagatc actaattttt atttaggtaa cttcggtgct
    11041 agcttcttag gggtaatggt cacattaggt gtcttcacta ctgctatggg actcgttgtt
    11101 tcttttgcac aagatttcca taaactgttc cctaaagtca gttatatgac atggctgcgg
    11161 ttaacgactt ttgtctcttt tgtcgttgcg aatgccggat tggataatat catccaatgg
    11221 tctttaccag tattgatgct tttgtatcca ttgtcacttg cgttaatctt actttcctta
    11281 acagcaaaat tcttccaaaa gagtccattt gtctatcaag taacgatgtt cttttctgcc
    11341 gttccagccg tattggatat gttggcaaat tcgcctgcat tagtgagtca gcaaactgtc
    11401 gtagcaagct tactaggatt ttatcatcat tatgttcctt ttgctgcatt aggtcttggc
    11461 tgggtcgtcc caacgttgct tggttacgct ggcagcttgc tcttttatta tgtatatcgc
    11521 gtatcaggat ataaacaaga agcaagtgag ttaccagaag aataaccaaa tcaaacacaa
    11581 tttaacataa gagatattaa acaggaaaaa aaacagctag aagaaagaat aagattcttt
    11641 cttctagctg ttttttagtt ctttttttac gaacacaaaa a
//
LOCUS       DACDIE010000076        11526 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.076, whole genome shotgun sequence.
ACCESSION   DACDIE010000076 DACDIE010000000
VERSION     DACDIE010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.076"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            325..882
                     /locus_tag="IV250_002365"
                     /pseudo
     CDS             325..882
                     /locus_tag="IV250_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            940..2979
                     /locus_tag="IV250_002366"
     CDS             940..2979
                     /locus_tag="IV250_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAP8769613.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(3088..4239)
                     /locus_tag="IV250_002367"
     CDS             complement(3088..4239)
                     /locus_tag="IV250_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP8769614.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4393..4923)
                     /locus_tag="IV250_002368"
     CDS             complement(4393..4923)
                     /locus_tag="IV250_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769615.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(5022..5372)
                     /locus_tag="IV250_002369"
     CDS             complement(5022..5372)
                     /locus_tag="IV250_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAP8769616.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(5529..7058)
                     /gene="ahpF"
                     /locus_tag="IV250_002370"
     CDS             complement(5529..7058)
                     /gene="ahpF"
                     /locus_tag="IV250_002370"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAP8769617.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(7068..7631)
                     /gene="ahpC"
                     /locus_tag="IV250_002371"
     CDS             complement(7068..7631)
                     /gene="ahpC"
                     /locus_tag="IV250_002371"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAP8769618.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(7775..8437)
                     /locus_tag="IV250_002372"
                     /pseudo
     CDS             complement(7775..8437)
                     /locus_tag="IV250_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            8621..10267
                     /locus_tag="IV250_002373"
     CDS             8621..10267
                     /locus_tag="IV250_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAP8769619.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            10258..11340
                     /gene="uxuA"
                     /locus_tag="IV250_002374"
     CDS             10258..11340
                     /gene="uxuA"
                     /locus_tag="IV250_002374"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP8769620.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
BASE COUNT         3860 a         2150 c         2185 g         3331 t
ORIGIN      
        1 tagaatatag taataacctt gttaggtgag gctcctatac aaacataggc tgttgcccag
       61 aaacgtcgaa agtcgccaat gggtaaaaca gaaactgttg gattaaggct ttttccaata
      121 gctaagaatg ttttctttac gttgtatagt gctaaaactc ggacgatgag atgatgctag
      181 gtcagaattc ttgttcgaaa aggtttctga ccttttcttt atatttttgt gactgactta
      241 tttgatgaga caaactcgct cctcacctga caagtggatt gctgtgtgag gaggagatgc
      301 ggtgaaaaaa gatcgattgg tggaggtcgc tcggattcac ttctggttgc acaatacaac
      361 cagaaaacac aacaagcaaa actgatctct atcatgagag attcatatgt cgatattcca
      421 ggttacggaa tgaataagat aaatgcagct tactcttatg ggggagttga tttattaaat
      481 caaacattaa aggaaaattt caaatttgaa gctccctatt atgcaagtat cacatttcaa
      541 gattttatcg attgcgttaa tgaactgttt cctggtggag tcaagatcga tgcagaaaaa
      601 tctctagatt tagatggcgt atatataaac aaaggagagc aagtgatgga tggtaatact
      661 ttactccagt atgctcgatt tcgggaagac aaagaaggag acttcgggag gatcagaagg
      721 cagcagcaag tgatcaaagc tatctctcag cagctgaaag acgtaacttc aatattcaag
      781 ctacccaaag cggttgggaa attacttggt agtatccaaa cgaaccttcc agaaagtgtg
      841 cttctggatt gtggtatgga tttcctcaaa gacatatgtt agaataaaca ggtataaata
      901 gtgaaataaa ggaatgacaa gcaagagaag gaggaaaaaa tgaaaagaag tgacaagcac
      961 ggcaaaaatc gaacaggcgc ttatattgcc ggcgcagtga tcttaatagc agctgcgggt
     1021 ggcggttatt tttactacca gcactatcaa gaaacccaag cagtagaagc tggagaaaag
     1081 acagttgagc aatttgtcca agctttgaac aagggagatt ataacaaagc tgcagaaatg
     1141 acatcaaaaa aggcggcaaa taaaagtgca ttatctgaaa aagagatctt agataaatac
     1201 cagaatatat acggtgctgc tgatgtcaaa ggacttcaga tatcgaatct aaaagtagat
     1261 aaaaaagacg attctactta cagtttttca tataaagcaa agatgaatac ctcattaggt
     1321 gaattgaaag atctttctta taaaggaaca ttagacagaa atgatggtca aaccacgatc
     1381 aactggcaac ccaacttggt ttttccagaa atggaaggaa atgacaaagt aagcctgacc
     1441 acacaagaag cagcaagagg gaatattata gatcgaaatg gtgaaccatt agcaacaaca
     1501 ggcaaactaa aacaattagg agtcgttcca agtaaacttg gagatggagg cgaaaaaaca
     1561 gccaatatca aagcgattgc ttcctccttc gacttaacag aagatgctat caatcaggcg
     1621 atttcacaaa gctgggtaca acccgattac tttgtcccat tgaaaatcat tgatggagca
     1681 acgccagaac ttccagctgg agctaccatt caagaagtag acggcagata ttatcctttg
     1741 ggtgaagcag ctgctcagct gattggttac gtgggagata tcacagcaga agatattgat
     1801 aagaatccag aattaagcag taatggaaaa atcggtcgct caggtttgga gatggctttt
     1861 gataaggatc ttcgtgggac aacaggtggg aaattaagca ttacagatgc agacggtgta
     1921 gagaaaaaag ttctgatcga acatgaagtt caaaatggca aagatatcaa attgacaatc
     1981 gatgcgaagg cacaaaaaat agcttttgac agtctaggag gaaaagctgc atcaactgtt
     2041 gcgacaacgc caaaaaccgg tgatcttctt gcgcttgcta gctctccaag ctatgatcca
     2101 aacaaaatga caaacgggat ctcacaagaa gattacaaag cttatgaaga aaatcctgaa
     2161 catccattca tcagccgatt tgcgacaggt tatgctcctg gatctacgtt taaaatgatt
     2221 acagcagcaa tcggtctcga caacggcact atcgatccaa atgaagtgtt gacgatcaac
     2281 gggcttaaat ggcaaaaaga cagttcttgg ggatctttta aaacaacgcg tgtcagtagt
     2341 gatgtatcac aagtagactt aaaaactgct ttgatctatt ccgataatat atatgcggca
     2401 caagaaacgt tgaaaatggg tgagaaaaaa tttcgtacag gcttagataa attcattttt
     2461 ggtgaagacc ttgatttgcc aattagtatg aatccagcac aaatttctaa tgaagatagc
     2521 tttaactcag atatcttgct agctgatact ggatatggac agggcgaact tctaattact
     2581 cctatccagc aagcagcaat gtattctgtt tttaccaaca atggcacact tgtctatcct
     2641 aaattgattg cagataaaga gacaaaagat aagaagaatg tcatcagcga aacagcagta
     2701 caaacgatcg tgccagatct gagagaagtt gtgcaagatg taaatggtac agcacattct
     2761 ctttctgctt tagggattcc gttggcagcg aaaactggta cagcggaaat caaagaaaaa
     2821 caggatgtaa aggggcaaga gaacagtttc ttgtttgctt tcaatcttga taaccaaggt
     2881 tatatgatgg tcagcatgtt ggaaaataaa gaagatgatg attcagcaac caaacgagca
     2941 tcggaactat tgcaatacct caaccaaaat tatcaataaa aaattgcttt ttaagtacag
     3001 taaaaggact gtggcgttat cgtcacagtc cttttttcaa ataaacggtt ttcacgatgt
     3061 ctctttcaca acttcttttt gatttactca cacttcataa actttttttg taaaatattt
     3121 gagaacaagt ggagaaataa gtgtacttaa taaaatcacg ataactaaag gtgaatagta
     3181 ctgtggttcg atcaattgac tttgctgtcc aatttgtaaa atgatcaaag ccatttctcc
     3241 acgcgaaatc ataccagcac cgaccatcaa tgaactatgt tgagaaaaac cggcaatctg
     3301 agcaccaaaa taacctccaa gcaatttggt caaaatcgct acaagcgtca atatagaaat
     3361 aaagagtagt tgttcactaa agtcagaaaa atcaacttct aatcctacac tgacaaaaaa
     3421 tactggtata aatactgcat atcctaaagc ctctacattg tggtatactt cttctttaac
     3481 ctttgtctga ctgacagcta tcccagcaaa aaaagcacca atgacagagc taagcccaac
     3541 aagatccgct acgtaagaca ttcctaaaca aataactagc gatacgatga taatagaaga
     3601 atttgcgtac actttttctg caaatgaaag taggaaaggc gcaatccatt tgactaataa
     3661 gaaaataaaa acaaaataaa ttagctgttc aatcaaaaca agcggtaaag aagcattgct
     3721 agaagcttcc cccgttagaa aagataggct gaaacttaga actaaaacga ctaaaatgtc
     3781 atctaccaca gaagcaccca aaattgttga tccttctttt gtattcacta cgtttagttc
     3841 ttttaatact tctactgaaa tactgacaga agttgccgca agaataatcc caaagaaaat
     3901 cgcttcattt cccgaaactt ggaatgcttt gcctgtcaac cagccaaaaa caaccgggaa
     3961 gatgatccca agcaaagcaa cgaacatacc aggtcggaaa tattttttca aaagagataa
     4021 atcactttcc agaccagcta aaaacattaa taagatcaca ccaatctccg aaaagtcatg
     4081 gactagaata tcaggtttga cccaattcaa tccagcttgg cctagcaaaa caccaacaag
     4141 taattgtcca atcactgctg ggatacctaa tctccgcgag atatgtgagc caatcgttgt
     4201 tgaaatcaat atcaggcata aaataccaat aaattccatt ctcatcattc ctttgtataa
     4261 aatatagccc gaacttagca taacatacat tattgaaaaa ataggaaaat atactcacaa
     4321 aaaaaccaac aatccaaaag atttttttgg attgttggtt tttatacggt ctattttaat
     4381 aactaaaaat gattaacgta tagtcatttt tcttattctg atgaaattga taatctcctg
     4441 gatgcccatg aaaatcaaaa taaagccgaa tatttggaat agaagcatca aacttttgaa
     4501 tggattgaac aataagacga atccgccaat caacaacaga atactactat aaacactcca
     4561 acttttagct tttgtattca tgctgaagaa caactggaac aagctgttga tgataacagc
     4621 taatccaaga agaatcggca ggatagaaac gattgcaggt gcaaaagcat agacgatcag
     4681 agctaagatc aggaaaaaaa gaccactgaa cagaccgata ctccaagaac ctgttttcct
     4741 tttaaaacgg taagcctcaa aaagattcaa caacccaagc aaaacaagat aggcggtgat
     4801 cagataaccg ataaaatgaa aaacagcaga agggtttagc acaatcgcaa tcccagaaat
     4861 aatataaaca gcagctctta ataaggcgta tttttgaata ttttgtttaa tcgtctcaaa
     4921 catataagaa aacctccttt gacttatttt aacgtgaact agtcatttta ccaaattaaa
     4981 tgttacaaaa tgagcgaagg agaagaatat ctctggaaaa atcaacttct gaggatcttt
     5041 tccatttttt tgccttttgc tagctcatcg atcattttat ctaaataacg aatttctttc
     5101 atcaagttat cctcgatgtc ttctactcgt acaccacaaa caacacctgt aatcaaggaa
     5161 cgtgaaggat tggggacagg ggcttccgca aaaaattgtt cgaaagtcac ttgtttttgt
     5221 agttgatatt ctagttctgt ttgggaataa cccgtcagcc aacaaatgat ttcatctact
     5281 tcctctttta tacgatcttt tctctccgct tttgctacat acataggata aacgcttgca
     5341 aaacttgtcg taaaaatacg attttgcgcc ataatcagtc acctttcttt ttttcaaatt
     5401 atttactact tatagtatat aagaaaaaga gaagaaaaag cgattattca aaaaaacaaa
     5461 aaggattgtg acaaaattct tgtcaccatc cttttttccg ctctctgact ttttacaatc
     5521 ttcatagctc aagtgcgcat cagataatcg aatgctccta aagctgcagt agctcctgaa
     5581 cccattgaaa caatgatttg tttgtatgga ctatctgtac aatcaccagc tgcgaaaacg
     5641 ccggggatat tcgttgcgcc atttttctcg gtcactattt catttcttgg attcatttcg
     5701 atcgttccat ctagccaatc cgtattaggg agtaagccga ctagaatgaa tactcctgaa
     5761 acagctagtg tatgcatctc catcgtacgg cgatcgatat aggagagaga ttctacctga
     5821 ttcgtaccgc tgatttcttt cgtcgctgca tgtttgataa cagtgacatt cgcaagagag
     5881 actagtttat cctgtaagat ttgatctgct tttaattctg gtaagaattc tagaacatat
     5941 acatgttttg ctaacccagc caaatcgatt gctgcttcaa caccagaatt accaccaccg
     6001 ataacagcaa tctcttttcc tttaaataaa gggccgtcac agtgtgggca ataagcaatt
     6061 cctttatttt taaactcttt ttcgcctggt acattgatag aacgccagtg cgcacctgtc
     6121 gcgatgatgg cggtttttgc tttaagtgat ataccgttag ctaagccaat attaataaaa
     6181 tgttgctctt tatttaagga aacggcctgt tgattcatca taatgtcgat agggtaacta
     6241 cgcacatgac tttctacttg ctgcatcaat tttggacctt ctgtgtaaga ggtaccaatc
     6301 atattttcaa tccctagtgt ttcaacgact tgtccgccga aagaatcagc gacaagtcct
     6361 gtacggattc ctttacgtgc tgcatagata gctgcactac tagctgctgg tccaccgcca
     6421 atcactaata catcataagg ttctttttca aacaactctt cttcaatcaa agggccagtg
     6481 attttttcaa caatttgttc gatcgtcatg cgtccgctac taaattcttc tccatttaaa
     6541 aacacagttg gcactgccat gattttttta tcatcgattt ctttttggaa catcccacct
     6601 tcaatcatcg tatgagtaat atttggattt aatacagcta atatattcaa tgcctgtaca
     6661 acgtcggggc aattatgaca agtcaagcta acataactgt caaaatgcaa tgtttgtgtt
     6721 atttttttga ttttaagact caaagattca ctgattttcg gtgcccgtcc actaatttgt
     6781 aacatagcca gtaaaaaaga ggaaaattca tgaccgaggg ggataccagc aaaaacgatt
     6841 ccactttctt tgccaactgg attgatagaa aaactaggtg tgcgcatcga atctcttttt
     6901 tcccaagaga ttttaggaga catttctgct atttccttcg taaattcagc tagtttttga
     6961 ctattttcac tattatccaa actaacagaa aaaacaatag gagattcaat cagtgctaaa
     7021 tattgttcga gattttgttt agtttctttg tctaacatat ccaccaccta aatctttcca
     7081 acaagatcaa aacttggttt caatgtttcg ccactttctt tccattttgc cggacaaact
     7141 tcacctgggt gggtacgtac atattgagca gcgcgaatct tatcaatcag tgcactagcg
     7201 tcacgaccga ttccatctgc attgatttcc atagcttgaa cgattccgtc tggatcaata
     7261 ataaatgttc ctcgctgagc taatccttgc tcttcatcca aaacatcaaa aatacgagaa
     7321 atctgatgag acggatcacc gatcatcacg tattcgattt taccgatagc atctgatgta
     7381 tcatgccatg ctttgtgagt aaagtgggta tcggtagagc aagaaaagac ttcaacacct
     7441 aaagatttca atgtactgta ttgttcttgt aagtcttcta attcagtagg acaaacaaaa
     7501 gtaaaatcag ctggatagaa gcaaagaata ctccagttcc ccataagatc tttttcactg
     7561 acttctaaaa attctccttg atgataagca tgtgcattaa attcttcgat ttttttccca
     7621 atcaatgaca tagtaaaacc tccaataata attatttatt tataattatt gtaatttagt
     7681 tggagttgca aagtcaatgg atattttgtt atttaataaa gattactcaa aaacgaaggt
     7741 taattataaa aatagaagag gtcgcaagaa aatgctactg ctggaaatac tcgggataac
     7801 gttgagtaac agtttcctgt tctttcagca tcatgtgtaa gtgcaatccc gtttgaaatc
     7861 gagcttcttc tggtttttta tcaactaaag ccttgagtaa atcataatgt tgttccatga
     7921 tcgtttccca tggaagcccc tgaatggtca ctcgtaacca gcggaaacgt tctaaatggg
     7981 tattatggtc atctaaccag ttccaaactt cttttcttcc tgcaatttcg aaacaagctt
     8041 catgaaataa attatctgca cggaaaaaag cgcgttcgtc atttattttg actgcttttt
     8101 cttgttcttc taaaatcgtt tctagcacta ttcgtgtttg tgtgtccaat ttggaacaac
     8161 attcaatcat gatttgctct tccaactgtt ctcggacaaa gcgagcattt tctgcagaaa
     8221 caagattaat tttggaaaca tacgtaccac tttgaggtat cgtttacacg agttcttgct
     8281 gacgtagttg aatcaatgct tcgcgaatgg gagtccggcc gatagcaaga tgttcctcca
     8341 gtgccttttc agatattttc cttccaggtt ctaagtcagc atagataatt tgtttacgaa
     8401 tcgcttgata agcttgtgat tgcaagctgc ttttcatctt tattcctcca aaatcatttc
     8461 tatgaacgta gtataacaga agaggtctga aaaacaatct ggtatgtcac attaactagt
     8521 aaatttgtat aaatagtaaa attttggttg acatagcgat ttttattgca taaaattcac
     8581 ttgaatacaa aaactaatat gttagtttag gaggggaaaa atgattgatt taaacaactg
     8641 gaggaacttt atccctgaat tatctcaaaa agaagtagaa atacctcatt ttgaccgtgc
     8701 atcattaaga gaaaatggac aaaaaaatcc tgtctggatc catttcggtg gcggaaatct
     8761 ttatcgagga tttcatgcag aaatcgctca acgtttagcg gatctaggcg aattgtccac
     8821 tggtgtcatt gtttgtgaaa cattcgatga agaaataatt gaaagggctt accaagattt
     8881 tgatgatttg attcttgaag tggtcatgcc aagcagtgga gagttcgtca aacgtatatt
     8941 agctgcgaca gctgacagtc tgtattgtca tccagattgt tctgaacact ttgataagat
     9001 gattcgacac tttgaaaatc ctagtttaca attagttact tttgctatta ctgaaaaagg
     9061 ttatcgattg aaagatacaa ataataaata tataggaccg attgcagtcg atataaaaaa
     9121 tggtccaaag aagccatcac atacaatgag tattcttgct gtgctacttt ataaacgttt
     9181 tttatctgga gcttggccga ttgcacttgt ctctacagat aatttttcac aaaacggtag
     9241 tcaattacgt acttctttgc taacgatcgc aaaagaatgg caaaaggaag gttatgtaca
     9301 aaatacattt atccaatact tatcgaatga agaaattgtt gcgtttccat ggacgatgat
     9361 tgatcgtatc accccgaatc cttccttgtc agttagcaaa atgttggcaa aagagggctt
     9421 ttcaaatatg tcgattttcc aaaccgcaaa aggaaataat attgctccat ttgtcaatac
     9481 agaggacatc cattatttaa tcatggaaga tcattttccc aatggacgtc cgaaattcga
     9541 aaaagctggg gtattcttga ctgatcgtgc aacggtagat aaagcagata cgatgaaggt
     9601 gacgacttgc cttaatcctt tgcatacgtc tttagctatt atgggttgtt tactggggta
     9661 taggagcata gctgaggaga tgaaagatcc agatttatcg ctgctagttt ctgaaatcgg
     9721 gtacaatgaa ggcctcccag ttgtggagtc tccaggtatc attgatccaa agatctttct
     9781 ggaagaagta attactgtgc gcttttctaa tccaaatatt cctaatatgc ctcagcgaat
     9841 cgccacggat acctctcaaa aggtggcgat tcgttatggt gagacgatta aaaaatatat
     9901 ggaaatagaa gttaaagacc cgcagacact tgtattcata ccattgactc tcgctgcttg
     9961 gcttcgatac cttattggca ttgatgatca aggaaaacca ttcatcccga gtccagaccc
    10021 actattgaca gaccttcagc ataagctttc cgatattcaa ctgggtacgc aagatacatc
    10081 acatattcat gagaaaatgg ctcctatttt gtcaaataaa gaaatttttg gtgtagatct
    10141 ttaccaagca gagcttggca gaaaaataga agaaatgttc atcaagatgg tagcaggacc
    10201 aaatgccgta cgaaaaacat tacaggatta cttacaagaa catggaggaa atttatcatg
    10261 gaaatgactt ttagatggta cggatcacag gaagaaaaga ttaccttaga tgaaatcaga
    10321 cagattcctg cggtcaaagg gattgtaggc acattgatgg atataccagt aggagaggta
    10381 tggcctaggg agaggatcaa agaactaaaa gaagaaattg agtctcgtgg gttaacacta
    10441 aaagtaattg aaagcgtcaa tgtgcatgat gccattaaaa ttggattacc ttcccgtgag
    10501 cagtatatcg aaaactataa gcagacaatc cgaaacttgg cagaatatgg aatagaagtc
    10561 atctgctata attttatgcc tgtatttgac tgggtgaaat ctgaccttga ctaccgctta
    10621 gaagacggct catccacgtt agcttttata tctgatgata ttccagatga tccaagagaa
    10681 atcgtcgcgc gcatcgaaca ttccagcaat gaatttggat tgccgggatg ggaacctgag
    10741 cgcttagctc ctatcaaatc cttgcttgaa gcctatgcat cagtagacga agaaaaactt
    10801 cgagaaaatt ttgcctattt tctccaatca atcatcccaa catgtgaaga ggtaggggtc
    10861 aaaatggctg tacatccaga tgatccgcca tactcgattt ttggattagc tcgaatcgta
    10921 aagaatcgag aagatttaga ttggttgtgt cgcatagtag acagccctta taacggaatc
    10981 actctttgta cagggagtat tgcagaagat ccagaaaatg atgtatatgg gattttagca
    11041 gaattttgcc aaagagatcg aatcccattt gcacatgttc gaaatattaa atttttagct
    11101 aatcgagatt tctatgaggc accacataaa tccgaatacg gttcattaga catgtaccgc
    11161 atactaaaaa caatgtatga caacggattt aacggataca tccgtcctga tcatggaaga
    11221 atgatctgga atgagacagg aagaccagga tacggcttat atgatcgagc attaggtgct
    11281 gtttatctaa atggattatg ggaagcaatc gaaaaaggaa acaaaagtaa agataattag
    11341 tgaaaaaaac tgtaatttta ttatgtaaat aagaaggatg ttcaaattct tgatacataa
    11401 atccggaggt tgttaataag cattcggatt tatgtatctt atttggtcta ggaagtaact
    11461 tgattttctt ttttcgattg atccatgagt gcttcttctt ttaattcaaa gatatattct
    11521 gagatc
//
LOCUS       DACDIE010000077        11420 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.077, whole genome shotgun sequence.
ACCESSION   DACDIE010000077 DACDIE010000000
VERSION     DACDIE010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11420
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.077"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            236..442
                     /locus_tag="IV250_002375"
     CDS             236..442
                     /locus_tag="IV250_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAP8769621.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(644..1645)
                     /locus_tag="IV250_002376"
     CDS             complement(644..1645)
                     /locus_tag="IV250_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8769622.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(1650..3563)
                     /locus_tag="IV250_002377"
     CDS             complement(1650..3563)
                     /locus_tag="IV250_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP8769623.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(3731..4237)
                     /locus_tag="IV250_002378"
     CDS             complement(3731..4237)
                     /locus_tag="IV250_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP8769624.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            4397..4837
                     /locus_tag="IV250_002379"
     CDS             4397..4837
                     /locus_tag="IV250_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP8769625.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            complement(4863..6020)
                     /locus_tag="IV250_002380"
     CDS             complement(4863..6020)
                     /locus_tag="IV250_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769626.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6023..6379)
                     /locus_tag="IV250_002381"
     CDS             complement(6023..6379)
                     /locus_tag="IV250_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769627.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(6677..7651)
                     /gene="sgrA"
                     /locus_tag="IV250_002382"
     CDS             complement(6677..7651)
                     /gene="sgrA"
                     /locus_tag="IV250_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAP8769628.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(7847..8686)
                     /locus_tag="IV250_002383"
     CDS             complement(7847..8686)
                     /locus_tag="IV250_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP8769629.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNRTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            8871..9758
                     /locus_tag="IV250_002384"
     CDS             8871..9758
                     /locus_tag="IV250_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAP8769630.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEGAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(10352..11047)
                     /locus_tag="IV250_002385"
     CDS             complement(10352..11047)
                     /locus_tag="IV250_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAP8769631.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(11031..>11420)
                     /locus_tag="IV250_002386"
     CDS             complement(11031..>11420)
                     /locus_tag="IV250_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP8769632.1"
                     /translation="FETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIELNQV
                     RKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVEDKE
                     MPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         3400 a         2287 c         1710 g         4023 t
ORIGIN      
        1 cttttggggg tatcctaaat cttttctatt atattcccat attttctatt attggtctag
       61 acgtatgatg gtaaactttg tacctatctg attgctgaat cattaattgc ttatcattag
      121 ccatctttgt ttcaagttcg ctagatgatc attttattca catcctaaca aacttgtcac
      181 atttatagat tcttaatcta tttatcatac aattataaaa aaggaggtgc tgcttatgaa
      241 agctatccat atttctttgc tttttttcgc tagtgcattg attttatttg ctattcatct
      301 ttgttcccag ccttatatca ataacaacgg aatattagtg gaaccagcat tcttttgctt
      361 accactcggc tttttgtccc ttcttgcctc cgcaggcttc ggatttgttg ctttttttaa
      421 acgatcaaaa caacaaatct gacttataaa atttacgtaa aagagatcgt gacaataatc
      481 gtgtgatatt cccaaatttc gaataaaagg tgccccttgc catctctaac tcctcagtca
      541 caagttacaa tattcataaa ccatgaaaag cttgtttatt gaaattgctc cttgtgactt
      601 ggagctaagt gacagattac aacctctttt gcatcataag actttactcc ttgaaaaaca
      661 ttactaactt ttgatatgtt tcttgcgctt gtttattgct gaaatcaaac tgatattcat
      721 ggttcaaaac agcatttttc tccgcataat ctacttttgt gactttcacc cccgctttct
      781 caaatacaga agttacttct tccatttgtt tcgtaaacgt atttgtattt ccatccgtta
      841 agaatgtgtt ttgcgtccaa cgatccgcat gtctgaccaa ggctaaactt ttgaccttct
      901 ctgattgttc aaagtcctta ttatcaaagt aagcccatcc acatttttca taaagaaagc
      961 tagagatttt atcacttact tgagtaagtg cagaaggatc caacagacca gacaaagaaa
     1021 taaacttcgt aatctgcttg ttctccaaag tcggatcaag atgatcagct tttttaattt
     1081 cttcattttg aagtgctaac aaatattctc cactaatttg tgcacctgcc gaatttccac
     1141 caatcgaaat attcgaccaa tctgcttgat cttttatctc ttttttagct gcttgaatgg
     1201 cttcgttcaa ttggataact ggtgtaggat gttttgcttc cggtgatcgt tcataatcta
     1261 tatttacgat gatttgctgc gtgtctgaac atagatattc caaataatca cgacaatcat
     1321 ttttatcccc tcctacatat gcgcctccat gcatccagaa aagaatcggt aattttttct
     1381 tttccacttt cggtgtgtag atatccatat tattattttt gtactttgaa ggataggata
     1441 tttctttttt tatatcgatt tgttcgattt tttgctggaa atcatctggt cgggcatata
     1501 cttgttcttt ttcacttgtg tcaaattgtt ttctaacaag caagctaatc ggctttggtg
     1561 tgatttgatt aatcaaaaat acagtgacta aaataactac gatgataact ggtactgcaa
     1621 tcactattcg cttgaaccat ttcattcatt taccctctct ttcttcgcca aatccatgtg
     1681 ataatcgtgc taacagataa aaatccacct gaataaatga gaagcgaatg tttcttttct
     1741 ccagttttcg gcaacattct tttttttgca tctgtctttt tttgtacttt cactgtcatc
     1801 tgaatcggtt gcgtatcatc acttgattga tcctctcgag gttcattctt tcccttattg
     1861 tctgcattct ctgtcggatc ttgctttttt ggtggagcta ctgggatttt tttgtaatag
     1921 aaaataatag ttggtatcgt ctcattaaaa atcccttcag tcggtccttc aacatttgct
     1981 aattcgtatc cttcgaatgt cttttgctct gtcatataat tttctccata agctccgtaa
     2041 agctggattt cgtcctggtc tttgagtaga cttttattgt tttcatccac atactttacg
     2101 tttatctgat catggaacac tactttcaaa aagtccgtta catgtttcgt gtaggcagca
     2161 tggtcaactt cctgtgcctt tccatgccct gctccttcta ctaataaaag atctttatac
     2221 cctcctgata actcatataa tttttgcgcg ttagacacag gtactacatc atctgctgtt
     2281 ccatggataa tcaatttagg caatgaatct gacattccaa tcgtaggtag agcagctttt
     2341 gtatccaaat gcatctcctg atcaaaatag tattgtttca aaaggcccat aaataaatct
     2401 tcatgctctt gatccccaat gatgtctaac tgatttttta tggcatccgg tagtgcgggg
     2461 gctactgtct ggctgtacgt aaaacgtagt tgttcatcaa ttgatgcaaa tccgcaattt
     2521 tcaataattc ctttgacatt gacaggaata tctttcgcta atacagacat catcgtagct
     2581 gctcccatcg aaccaccata aagtaaaatc ttttgagatg gccgctctcg aacttcttga
     2641 tttatccagt aaaggacgtc atctgactca tattgtccat aggtcacata atcgcctccg
     2701 ctattaccag tcgctctgga atcaacaaat aataaattat acccggcctt ataaaaatca
     2761 ttgtattctt ctgtaacgat cccgttattc caattccctc gaaatccgcc atggatcatg
     2821 actgttttat ctgaaccttg atcgacataa tatgcatata catccatcat tttgccatct
     2881 tgtttgttta gtacagtttg atgaagttgt tttgtatttg gatagaagcc tgtgttttct
     2941 gtaggcgttg tcccatcatc tgtacctcca agcatcgaca tcaacacact gttttttgca
     3001 aaagcgatat cgtacaatag ataagtagca atcagatgtt tgataatctc tttatctaca
     3061 ccataatcat taacagcaag ctcagtgact aataactcta ttaattttga cttgatgtct
     3121 tctgttggtc cattaagtat ttctgtcatc tgtttaccga agtaattcaa atattcttta
     3181 ccattgaaat tttcaccttt accgatatca tcattttctg tcccattctc tttgtcactt
     3241 actttagcag aagaggattc gtcgtttgac atttttgtag ctgttgcttc ttctgtttgt
     3301 tggttatctg tggtagcggt cgtttcttga tctgtgctag taacagatgt ctcaaccgtt
     3361 gtttcagtat ttgtttctgt ttgtgaagta ctcgatttcg tttcatttcc agcatcagta
     3421 gtttctgtgg tttctacctc ccgagattcc gattcggaag aaatgctctc tccatatact
     3481 gtccattgcg ggacgcttgt acttacagca aataagatga ccaacgctcc ctttaatact
     3541 tgttttgttc tcaagctatt cactcctttt tatgtttcta ggagaattat atgtaaacgc
     3601 aaacattttt tatacaaaaa ataaatggtt tatcccataa aaatagaaca ggattatttt
     3661 ctcattttac gtaataaagc agaatggata aaccagtatc cattctgctt tttttaaacg
     3721 aagaaacaac ttattgctcc aaaaatgtca gaaatcgttt atcccaaatt ttctcgtaaa
     3781 aagcgattgt ttcctttgct ttcataaaag gattatcata agttgtcgtt gctcctctac
     3841 acatcataag ctggtatcct aatccatgag caaacttcac agtcgtatct atacaatact
     3901 ctgcttgtgc cccacataat tcgatcgaat gacagtccaa ttcagttaat aaaacactca
     3961 aattagtctt ataaaatgaa ttcgcatggg tttttctcac aaaataactg tgctcaggaa
     4021 tagataattc cgacaatagc tcccatggtt ttgtttgtgg cactaattga gcatcttcgt
     4081 gttgaacgaa aataactggt ttcccaatcg tttcgtatgt tttgattctt ttattcacta
     4141 atgctgtcac tgtttctaat tcgtgtatcg gcgaagaatc aaaacatacc cctttttgta
     4201 aatcgatcac tactattgcg tcggctgtgt ttgacatact tgtgccttct ttcttttttc
     4261 ttgatttaat agtatttctt atctagctag ttccattata cgtgacaaca ttttcttatt
     4321 ttacacctct aaatggcact tttcgactat ttcttcatgt tattatttca taaatactta
     4381 gctattggag gaatgtatga tgattaaatt gtatacaggt tctggctgct cgtcttcaag
     4441 acgcgcaaaa cgttggctta tcgtccatga tataccgttt atcgaacaga acttaattcg
     4501 tcaaccacta accaaaaatg aatttatcca cttactaaaa ttgacaaaca acggcgtgac
     4561 cgaaatgctc tctactcatt ctattagtta taaaaatctt gttcacagaa tagatgaact
     4621 cagtttgagt gaactatata ttgagattcg tacatcccct tctttattga aaaaaccgat
     4681 tatctacgac caatctgcaa aacttttgac tggatttagc gaagataaaa tcaggatgtt
     4741 cctttctaaa actaaaagaa agcaagagct caacgaactc ttgctttctg gtcatcttgt
     4801 cgattttaaa gaaaataatc tttttgcaat gtcttaattt tggcataaac aatctgcttt
     4861 tcttaattaa cgaaatccaa tttttccaat atggatctat ttttctcatt cctgattttt
     4921 atgatttcag ctttcagacg cgcccaatca ttttttgtag gttgtccatt ccagtaaaat
     4981 atctgtgtat tcgtttgatc atatgccttt ttgtcgatag gataatcgct aatgacaata
     5041 tcaggtaact gatcagcgga tgcactatat tttattgggc gttcagtcat agataatatc
     5101 cttttctttg cccataattc ctgtgtttta ccatactttg attgaatata gaatatgaca
     5161 ggttgttcgt gttttataaa aatatccctg actaacatac agtattgaaa aatcaaccgt
     5221 ctgttctttt cataataagt attaagctct ggaattctgc gagctacccc actaaaaaac
     5281 tgttgcatga ttggatacat cactggaaat atatgctgaa aatctctgtc ttctgctgat
     5341 tttccaaaag catccacctt cttttttgtc ccaaaaaccc ttgaatacaa atggatcatt
     5401 gccaaattga catatagata atttttttct gaagttgtaa aacaaatctt gaagtttttt
     5461 tcaaaatttt ctacaataga cgattcaatc cagccccaac tttcttgtac tttatctgga
     5521 gtatccaaca aatcttcttc caaataggtg ttcattacac caaacataaa ataaaggtaa
     5581 gcttgctctg ttggagtgat gtgattcatc gaccagaagt ttgcttcaaa aaaaggctct
     5641 agtatgtaat gcgtaaactc ttcttttgtt atcaatggat gcacaaattt gttcatctct
     5701 tctgggacat acgtaatcga gtggccttgt ttcattcgtt gcaacgaaat tcttagtagg
     5761 ctgatccatt tctttctatc tatactacgc gtatatggcg catgtttcgc aaatttttct
     5821 aatagttgac gtttattttt tatacttgat aatatatcag aatgattttc gtcgaatgac
     5881 tgccaataca tcaagtgaaa aaagtatcgg atacggtatt cttctccttc tatcggatta
     5941 ccttttttca agctgcaaga taaatgatag tttttcaaaa tagtattaag ttctcttaat
     6001 tttctttgaa tcgtacgcaa acttatataa ttttttgact aagattttct aaggattgat
     6061 aagtaccttt gtaaagtgaa tccagtaaaa tataaccaat cgttccctct aaaagtatag
     6121 aataaacttt actcagtaaa aactgagaag aaaactcgac ttttatcatc ttgttttctg
     6181 aaagaaaaga taatttatat tctggatacc tttcgcaaaa cgattctgct tgttgaatca
     6241 ccgaattcat cttagccagg gtgtagttta gatctaaagc gatatccttc accacatata
     6301 atttctcgtc tctttcataa caatagttca aaaattgata tacttgcctt tcattgatat
     6361 caataaattc ttcaaacaaa gtacatactc ctttttatct attatttaga cttattccga
     6421 cagctacatg aataacgtac aaatagattc gttactgatt tttcagagat actaccatgc
     6481 gcagtgtgca ttgtcaatta ttcttgctct tattttccat ttgtgttcct gttatttcct
     6541 gtttctcaaa tatgttagta aacaaaaata gatctggcaa agctacaact tcgccaggca
     6601 atacagcact ctcgaatgaa atattcactc aaaaatctat cgttttattc aattttagat
     6661 ctgttgcttt tattctttat ttcactcgat ggaagagaga acgaaaatag cttgctccta
     6721 caatcaaaat gagtaacaca cccatccatg taattagctt gttcaaggtt ctactaccag
     6781 tttctggtaa atcgcttttt ctattatctg tttctttcgc tttttcttga tgatcaattc
     6841 cacgagaaac aaaatcaggc tgttttagca cactttttag gttgctccct gcatttctct
     6901 tttgttcctt agttggtatg ataggtacca aatcaagttc attatttact tttcctgtgc
     6961 tgctttcgct gctcgtgctt cctgtgctac tctcactact catgcttcct gtgctacttt
     7021 cactactcgt actctccgta ctgctttcgc tgctcgtgct tcctgtgcta ctttcactac
     7081 tcgtactctc cgtactgctt tcgctgctcg tgttttctgt gctactttca ctgctcgtac
     7141 tctctgtact gctttcaccg ctcgtgtttt ctgtactgct ttcactactt gtgctatccg
     7201 tactgcttgg cggtccaaca actgtccaac tgcttggttt caaattctcc gtgatcgtca
     7261 tctgtccatc ataaaaaaca atcaaatcaa ctttatcttt actcttgatc tcatcaatcg
     7321 atagttctgc agaaatcatt attgtatact caataccagc ggtccattgt acgtctttta
     7381 aataaacttc cccaataact ttttctgaac ttccattacc atcccaacca tatgttagaa
     7441 cagcatgtac atcactagca ttcacgcttt tgtttggttt gactggaata gtaatgactt
     7501 gttgtgtcat gtcataaaca atccgatcag ttttcacagt aaatgaaaga ttctcatttg
     7561 cccgttcatt tgcagaagta ctgattccaa tcattgctgc aattatactc agcccaagaa
     7621 taacaaataa tacggtcgct gttttcttca tttctatcac tctccatata attaccttct
     7681 atttgataaa ggaattatat aaaatgaaaa tgatgagaac acgacagtca gttgtgaaaa
     7741 tctaacaaaa gatgtcgtgt attcgcttga atttgtttac tttttcgttt aatcaatgac
     7801 agacttaaaa aagcaagacc gatatcagcc ttgctttctt catcttttat tgataaacag
     7861 cagtgtattt cttcatccgc tttgttttat atgcatagag taacaaacca acaatcaaga
     7921 ctaacaagaa taatgtagtt gatatagatg cttcagcccc aaagtttccc ccagtaatta
     7981 ttgtattttg cgattggatc agagtggaga aaaaaccttg ttcttgcgtt ccgctgactg
     8041 gtatacccaa aatagctcct tgtgtccgat tccaagtaat atgagcacca atagccgtaa
     8101 ataaacttcc tgtactgtag ataagtgcgc tgaaaaagca acctgctaaa aagatattga
     8161 aaactgccag aaatgtcatg ttaggattca atccgtgcaa aagagcaaat aatgcagcag
     8221 atccaatcat tgctattaac ggaccaaatc taagcatctg atgctgaaaa aagccacgga
     8281 ataaaagctc ttctgatgcc cccactgcaa taaataaaat aaaactcgtt atcagcggca
     8341 caatgtttgc gtttcctgaa tctaaccgat acgcccccgt caaatataat ggactagtaa
     8401 taagcagaaa cactccaatt ccaacaacga gacctcccgc taaatacccc cagtcttttc
     8461 ctcgaacaga aacaccataa tcttctattc tttttccttc gaccttacta gcaagaaata
     8521 aagctagtaa aaaaacagct aaatgtccga ccgtaccggc aattgctcca atccattcac
     8581 tatttccaat aaacatatcc gcaagtgcat tattgacaag aattcctaaa acaaaaccaa
     8641 ctaagactat taaaacagat ccaatcaacc gaataatcct gatcatcttt catccctcct
     8701 ttatattaag tttaacaacc cttttctttc ttgtctcgtg aaaactatgt aaatgttctc
     8761 catcatagag agaatagatt tttacgaact aagaatatct acagcaacct tacgaaaatg
     8821 aaagactgtc tccctaattc ttctgttaga ctgtttttaa taaggaaagg atgggaaatt
     8881 ttttgaaaaa acaactacta attatgtgcc tttcagcatg tgcaatttta ctattctcag
     8941 gttgtagcaa gtccaatgaa acgattgcaa cgtttaaagg tggaacactt acagttcagg
     9001 atttttatga taacccaaaa gttaaatcaa ataatcaaac actattaaaa aaaatgattg
     9061 tcttcaaagc atttgatgat gtatacggaa aagagatttc ttcaaaggaa gtaacaaaag
     9121 aatataatga acaaatcaaa aaactcggtc caaactacaa ggaacaatta aaagcagttg
     9181 gccaaacaga agagacatat aagaaactct ttaaacagat gctggctttc caatacggtt
     9241 tgaaagccaa tgtaaaatta acagataaag atctagacac tgcttggaaa gaattctatc
     9301 cagaagttag tacacagatc attctatttt cgactaaaga aggagcagat aaagcaaaaa
     9361 aagaagcaaa tgaaggagaa aatttctcca agcttgttca agcttatgga aaaaataaaa
     9421 cattaaagga aactgatgga aaaatgaatt ttgattcaac caatccagag attcctactg
     9481 aagtaaaaaa agcagcattt aaactaaaaa acggggaagt ttctgatatc attcctgtaa
     9541 cagatccaac gacttatcag caaagctatt atctagtaaa aatggtaaaa aaacaggata
     9601 aaggttctaa taaagacaaa tacaaatctg agctagaaaa aattgctacc gaagcaaaaa
     9661 ctcttgatac ggaattcatg gataaaacaa tccgtaaagt gatgaaaaaa gacaatgtaa
     9721 ctattaaaga tccttatgta aaaaatatat tcaaataaac aaatttgacc caattgggga
     9781 tgtggcaaaa tttgagaagt gatttttgga aattccttct tatttttgag gcaaaccact
     9841 tttgccacaa cttcatcggg gccttttttt atttgtacca aaacaaatca ctggatttgt
     9901 tacaatcatg aggatataat ttaaaaaagt tttttatttc tatcacatga atcaatgcgc
     9961 cattaagtag attctttcat gcttccctat tttttcactt gccgtatcgc ttcaaaaaca
    10021 actatcataa actcaaactc gactgaaaag ttattttaaa aaagactaaa aatatccctt
    10081 ctggacttgt tcttcttatt ctgttatact agagaaacac gaaagatatt tttatgcgac
    10141 acttttcatt tgtttttatg aaatttattc aaaactggcg taacttcaga gttacaagga
    10201 tgtattagag gttagggaat acatcgttta aatgagaaat aagtaatagt tagtaatagt
    10261 cacccaaact ttaaattaat gaatattgaa tcgtcagata gcctcctcga gttttcgaga
    10321 aggctatcgt ttattttatg gagaaattga ttcagaagta atcagcgacc ttttttagtt
    10381 caccaatcgt ttctgcttgg aaagtttgca cccaatcttc ttggaagccc ctcactgctt
    10441 tatcaatcga gggcatctct aatacttgat ctagtagctc tttacctaac gttatcgcat
    10501 gagctccaga tcgaatggct gtagtgactt gttcacaatt tttgaaacta gctgctaata
    10561 tttttgttgg tctttgcgta cgttcgatct cagtccgcaa cgaatcaatc actttcatag
    10621 catcaatgcc ttgattttgc atcctgttat aataaggtgc aatataatct gctccattat
    10681 taatagctaa atacccttgt aattctgtgt aaatagctgt accggtaatc aaataacctt
    10741 cttttttcaa ctttttgatc gcttgaaggc ctgcttgatt caccggcacc ttgataaacg
    10801 tatcttttcc cagttcttct atgataactc gagcatcctc tatcatttct tcttccgttc
    10861 ttcctattgt ctgaatatgg ataggaatct gtccgattat ttttttgatt tctttcatat
    10921 gcgagaagaa gtttgacgct ccttcttttt tcagaattgt aggatttgtt gttacgcctg
    10981 ttaaattcaa aaactgttga caagaataaa tactttttgt atttgctgta tcaagcatga
    11041 tttccatata gctttccccc attctgatcc tcttgatcga ttttaatgat tgtccccttt
    11101 cttaaatgcg gcatttcctt atcttctaca tacattgttc ctggaagttc cggctcatga
    11161 gatccatcaa attttatcgt gatatgtcct aattcattca aattagtttg gacaacatct
    11221 cctactgctg tgattacata catttgttta tcaaaacaga gcttcatatc acaagttatt
    11281 tcttttctta cctgattcaa ttcaatctta taacaataat ctgttaatgt ttccggtgct
    11341 tcatctccaa ataaaatcat tattttttct ttcctgaata cttctgcttc tataccaaca
    11401 tctgttacaa ccgtttcaaa
//
LOCUS       DACDIE010000078        11379 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.078, whole genome shotgun sequence.
ACCESSION   DACDIE010000078 DACDIE010000000
VERSION     DACDIE010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11379
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.078"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..237
                     /locus_tag="IV250_002387"
     CDS             <1..237
                     /locus_tag="IV250_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAP8769633.1"
                     /translation="PRKNMDKSEKLDHSLLGKQRKTVETVFSSLEKLGCQNFNSRSVK
                     GLESRFESILLAYSVLLSRAQRRFGGTLRYSLGY"
     gene            655..1878
                     /locus_tag="IV250_002388"
     CDS             655..1878
                     /locus_tag="IV250_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAP8769634.1"
                     /translation="MESYQFQVFKLVPSLERRGNRMKITEKNVNPKFETTESNLKKFD
                     LTVTLSNQLVDSIVEMDAMPQKIYLYAIGHLDPKNPPFNNTVKVSKKALFALMSSSSS
                     SSKHSRLKQVIEKLHQQSVIKIQRTKPNGKTEYKVISPLEITEFNDFSDAVSIKFSSS
                     IMPLLTNLEDHFTQYTFDQVVNLTSKYAISFFQLFTKDFNLYEANRDNLTWSTKSLDR
                     LKNPVFSIADLRKKFSIEKKYKAFRDFERRVIKEPIAQLNKYTDFKIDYELIKKGRTY
                     TNIKFLLTRTMSVPVPYKQEQRDPAYLKGTEDKTEKMNQSYLKAMNSKYTKLLIKNFL
                     ISSADLCDLKLMSRLQNKVYPLYDRLSKDRGFESVVNHLSYVATHKEDYTIKNTVKYL
                     EKSITSYLPYVNLYD"
     gene            1913..2680
                     /locus_tag="IV250_002389"
     CDS             1913..2680
                     /locus_tag="IV250_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP8769635.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFEDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LNIDFAKVRKGRNYNFAVQFNISSEFDEKTYREKKLG"
     gene            2872..3270
                     /locus_tag="IV250_002390"
     CDS             2872..3270
                     /locus_tag="IV250_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="HAP8769636.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
     gene            complement(3576..3971)
                     /locus_tag="IV250_002391"
     CDS             complement(3576..3971)
                     /locus_tag="IV250_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769637.1"
                     /translation="MKQEKRKMKQTQTETVNDTLADQLAGYYKLFEMGKAQRLIHYLW
                     KYKILFLFWESTLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVIS
                     DAGIERNIKRVSFKITLNKPTDKKLSEAF"
     gene            complement(3972..4349)
                     /locus_tag="IV250_002392"
     CDS             complement(3972..4349)
                     /locus_tag="IV250_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769638.1"
                     /translation="MKSKKETEPMKSTHLNYFSRTLINNLLKDTTGISVSTKHFANDL
                     IIFEDNNLRIEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKK
                     AKLLFEFQTDYHNELLAQSEILD"
     gene            complement(4339..4734)
                     /locus_tag="IV250_002393"
     CDS             complement(4339..4734)
                     /locus_tag="IV250_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="HAP8769639.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            complement(4801..4986)
                     /locus_tag="IV250_002394"
     CDS             complement(4801..4986)
                     /locus_tag="IV250_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HAP8769640.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            complement(5068..5322)
                     /locus_tag="IV250_002395"
     CDS             complement(5068..5322)
                     /locus_tag="IV250_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769641.1"
                     /translation="MNYTDEEQITLEYVFTNEEGEEIEKVLIISTKDLEKALAYEPYE
                     STVTVIEFLDTYTSNDTWEIMNICNANNIQFAEKTNKSFY"
     gene            complement(5344..5589)
                     /locus_tag="IV250_002396"
     CDS             complement(5344..5589)
                     /locus_tag="IV250_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769642.1"
                     /translation="MWTLEGKTFAEIWTEFIEVPTRIDPITSEKVLDADFYNLKAGTS
                     LECVWMYLEEHFKGVTLGHVLYHNGAEWDAIGNIHFS"
     gene            complement(5596..5880)
                     /locus_tag="IV250_002397"
     CDS             complement(5596..5880)
                     /locus_tag="IV250_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769643.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGIVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKNSFIGKLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            complement(6371..6910)
                     /locus_tag="IV250_002398"
     CDS             complement(6371..6910)
                     /locus_tag="IV250_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769644.1"
                     /translation="MYNKSVNKLINLQKERFFMGWYGAYSSANEVIEEKKGYAKNGLE
                     IVDTKVTKTYGIILFKDAEGTYDIDSFIFRDEMYKPIYFLNDSPAYFKKIPKKWLKFF
                     VGDQEKMVKRQLDRIKEESNKPKLDDLLVAGKTYSIWDGSYVAVYQYKLKRSFIFRLQ
                     DGSLTRFTRLSAGDVKEVA"
     gene            complement(7023..7289)
                     /locus_tag="IV250_002399"
     CDS             complement(7023..7289)
                     /locus_tag="IV250_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP8769645.1"
                     /translation="MSYQVRYEREAQKALKKMDKFQAKVILNWIEKNLVGTDDPRKHG
                     KGLTANKSGYWRYRVGAYRIIADISEHEVTILILSIGHRSEVYK"
     gene            complement(7291..7512)
                     /locus_tag="IV250_002400"
     CDS             complement(7291..7512)
                     /locus_tag="IV250_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HAP8769646.1"
                     /translation="MPVISIRVSKEEKDFLEKVAEFNGDNISEFMRSNSLQTAETLVD
                     FATYKELMKEHEENDQSISHEDMLKELGL"
     gene            7732..8550
                     /locus_tag="IV250_002401"
     CDS             7732..8550
                     /locus_tag="IV250_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8769647.1"
                     /translation="MEKKLSIITGNIEKGGTGKTTTIYNLGGYAVENLGMKVLYIDED
                     KSQNLSKRFPDAFSKAKRENTINHLYLHGSAEPLAINENMDILVAGEDLKQVEMQMRD
                     MPNNRTLLFRWIVVNYEQLKEKYDLILIDTHNDAGLLAQNAWAVSDLVVGISDPSKDG
                     FEALLKLGKDIDALAKQLIDIKTGETLMETKYATIGSRIKHNTNSSREFLELVPTLSN
                     YLGYVQEKELLNATSLEGKNIAEFAKDKNIYRKNKDFFESTFTLYNKIIDTAQE"
     gene            8554..8850
                     /locus_tag="IV250_002402"
     CDS             8554..8850
                     /locus_tag="IV250_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769648.1"
                     /translation="MIEVKENRFKKFAEAEVEEVPEVVEAVEEPKQTPKETRSSLVDK
                     LKSDSSEKKKNYQFTLQPSQRKKLSKISEKLGIRSDSALLGEMIDNLYDEVFGE"
     gene            complement(9031..9375)
                     /locus_tag="IV250_002403"
     CDS             complement(9031..9375)
                     /locus_tag="IV250_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769649.1"
                     /translation="MNKGKSERYVVIINREKVSELPLAEFLESEYETLGVKYSQTIKK
                     ALNLYRRVKNNEVTIIEAGNDSKVAEVKNKPTDHNPIQQEHPSTVTSGHSAKKEKPML
                     KGFNAPSNKDLL"
     gene            complement(9395..10450)
                     /locus_tag="IV250_002404"
     CDS             complement(9395..10450)
                     /locus_tag="IV250_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParM/StbA family protein"
                     /protein_id="HAP8769650.1"
                     /translation="MKEPLLFSVDLGNKSVKEMSTHVSPLSLPSRILNKNIINSGRFS
                     NIGSSLKRSLNVSEYSLVNTTESYYFGKDIHELGKDDYVIESLGFGISRYKQETYQNI
                     LSFSLANLARLEDQSDDVLTVNLVLGLPSSDYTETIVKYVMQQATRQHSVEVDGKAYN
                     INVDTVWVLPQPIGTLYNLLLADDGSVQDETLISQKIGFVDIGGGTKLFDVVQNFEID
                     ENNREQKDTGIFTLYNRIVNMLDLPISPNAFQLEKIIREGKEKGVYLFKPSGNESFDI
                     SEVVEREIKTYTNMVVAEVLATFKNFTTLDKVVFTGGGASIIDKQLVKDKIPHTLFVE
                     DSEFSNVKGFYKFGLTQ"
     gene            10666..11064
                     /locus_tag="IV250_002405"
     CDS             10666..11064
                     /locus_tag="IV250_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769651.1"
                     /translation="MRLRRNKNYTTIILEDFTDQELLIVGKTKADFIEKGNASIVKAS
                     KVAVEKYFDSLEESAKEIFNGEKAGSYELDGIRFSLIPTTENIIPKKLYYIFSSEKYT
                     SMKPCILDWQWLIDAVLDVKCSKYNITLGC"
BASE COUNT         3793 a         1764 c         1946 g         3876 t
ORIGIN      
        1 ccacgtaaaa acatggataa atcagaaaaa ctagaccaca gcttacttgg aaaacaaaga
       61 aaaacagttg agactgtttt ttcttcctta gaaaaattag gttgtcaaaa tttcaactca
      121 cgttctgtta aagggctaga gagtagattt gaaagcatat tactggctta cagtgttcta
      181 ttaagtcgag cacaacgacg ttttggagga actttgagat attctttagg atattaaaat
      241 taactagcac cacgggtata tttataatat atattttaat ctttaaaact ttagcaacct
      301 ccaaaaaggc tttatatcaa tcttaaatag attttagggt ggacataaag cgatgtgaat
      361 gtggacaaaa agcgatgtgg tattccgaat atatccaaaa aaagaaaaag tatgtgaatt
      421 tatttctaaa aactagaaaa atatttgttt tttaattttg gtaattttta taatttcgta
      481 tttttttgaa aaggattttt attttgaaaa tgttctaaaa tagcgatttc tgctgtattt
      541 tcaatgaaat ttgattggaa aaaataaaat gtgttttact gacattgagt tatgttataa
      601 atttacgata catttcttat tgtaacgtat cgaaaacagg tctatactta ttcaatggaa
      661 tcgtaccagt tccaagtttt taagctcgta ccaagcttag aaagaagagg caatcgtatg
      721 aaaataacag aaaagaatgt aaacccgaaa tttgaaacta ccgaaagcaa tctaaaaaag
      781 tttgatttaa ctgttacttt atcgaatcaa cttgtagata gcattgttga aatggatgct
      841 atgccacaaa aaatttatct ttacgctata ggacatcttg accctaaaaa tccgcctttt
      901 aataataccg tcaaagtatc taaaaaggct ttatttgcgt tgatgtcttc tagttcatct
      961 tcttcaaaac attcaagatt aaaacaagtt attgaaaaat tgcatcagca atctgtcatt
     1021 aaaattcaaa gaactaaacc taacggtaag actgaataca aagtcatctc accgctagaa
     1081 attactgaat tcaatgattt ttctgatgca gtatctatta aatttagtag ttcaattatg
     1141 cctttattaa cgaacttaga ggatcatttt acgcaatata catttgatca agtagttaac
     1201 ttgacaagca agtatgcaat cagttttttt caattattca ctaaagattt taatctgtat
     1261 gaagcaaata gagataactt aacatggagt acaaaatcac ttgatagatt aaaaaatcct
     1321 gttttctcca ttgcagattt acgaaagaaa ttcagtattg agaaaaaata taaagctttt
     1381 cgagactttg aaagacgtgt aattaaagaa ccgattgccc aactgaataa atacactgat
     1441 tttaaaattg actatgagct tatcaagaag ggtcgtactt atacaaatat aaagttcttg
     1501 cttacacgta ccatgtcagt tccggtacct tataagcaag aacaaagaga ccctgcttat
     1561 ttaaaaggca cagaagataa aacagagaaa atgaatcaat cgtacttgaa agctatgaat
     1621 agcaaataca ctaaattact aatcaaaaac tttttgatca gttctgccga cttatgcgat
     1681 cttaaactta tgagccgact tcagaataaa gtttatccgc tttatgatcg tttatccaaa
     1741 gatagaggat ttgaatcagt tgtcaatcac ctatcttatg ttgctactca taaggaggat
     1801 tacacaataa aaaatactgt taaatatctc gaaaaatcaa ttacgagtta tttgccttat
     1861 gtgaaccttt atgattaaca actgaaaaaa tgcgtgaagg aaggaaaaac ttttgaaaaa
     1921 agatgaactg acaaagtaca atgaggagtt cttagcaaca attgacatga aaatggattt
     1981 aaagtcgtat gaactttatc aattgctgtt cagttatatc tcacccacaa agctaaagaa
     2041 aacaggcgta gtagatttac ctgttaaagc gattattaat tacatggatg ttgatcaaaa
     2101 tcttgaaacg gaactaaaaa ccatagttaa cactttgcaa agacaagcag tttatgaaat
     2161 ttcgaatcta gacaaaaaat ataagacgta tactactgta tctgctattg atcggattca
     2221 ttgggattta gaagagcata gtgtatcttt cagtttttca gaagatgtaa ttcccttaat
     2281 tgtctcaatc aaaaagaagt atccgttctt tgtttttgaa gacttagcat cttttcaaaa
     2341 aaaatatagc atcgtgttat acaaatattt ggcgatgtat tataaaaaat atcttatcgc
     2401 caaaaataaa ggagattttg agaaagctga aatatataag aatcccctga ttgaaatagt
     2461 taaaattcgt gagattacga aaacagaaaa tgagtataaa acaatatcga attttgatag
     2521 acgtgtttta agggaatcag ttgaggaaat taatgaaaaa tcaatgctga atattgattt
     2581 tgctaaagtt agaaaaggca gaaattacaa ttttgctgtc cagtttaata tttctagtga
     2641 gtttgatgaa aaaacttata gagaaaaaaa attaggttaa tgcaaaatat atcttactcg
     2701 tttgaagatg cttcagacgt atgctaagat atatagtgcc ttgggagcaa ggtattaatc
     2761 tcctttagag gttaattagc ttgtgatcgc ctatcaattt ttttggtagg cgattctttt
     2821 ttttgaaaca aaaaagcaaa aattattttt taattagaga ggtgcaatgc gatgaaatac
     2881 tttttttgtg cagtattcga atggaaagga aatgaatatg cagttaaatt cccagatttt
     2941 ccagacttat atacacatgg tgaaacttta gaagatgctt ttgagatggc gcaagaagta
     3001 ttggaaggat acttggtgct tttagagttg caagaaaaag aaattccatc gcctacgcct
     3061 gttcaagagt taaatgcaag aatcaagcga tctgagaatg ttaatttatt tgtcgtaaaa
     3121 gctaatacga aagttgctaa agctcgaaac aagcaaaatt atgatcgaaa atcagtgact
     3181 gtggaacact atttaaatgt tttagcggct gaagagaata ttaatctgtc gaaagctttg
     3241 aatgatttgt tgagagaaaa gttgatttga tgtctctcag atgatcatat taagcaaaaa
     3301 cagcttgttt taggacgtat ggatttaaga aagacaaatt atcaaagaat atattaaaag
     3361 ctctcagaag ccttctgaga gcttttaaat tgctatgtat tgtactgcat ctgctcaaat
     3421 tgattttctt ttcatgctta ccatgtatct agttttccat caagtaattt ttagatatgt
     3481 attaagtaat aaatagcaaa tttgcttgat ataagctcga attaggtgct ttataatgtg
     3541 ttaggataaa ttatacaaga aaatgtcaaa agctctcaga acgcttctga gagcttttta
     3601 tcggtaggct tgtttaacgt gatcttgaaa gatacccttt tgatgtttct ctcgatccct
     3661 gcatcgctaa ttactaagtg ttcttcaagt gtgatgtagg tatataaatt aagcatcaaa
     3721 atggtgtaga taagcaaaaa actccctaaa actaagtgtt gattataaaa atctgactta
     3781 taaaaaagaa ctgagaatgt tagtgttgat tcccaaaaaa gaaacaaaat tttgtatttc
     3841 caaagatagt ggatcaagcg ctgtgcttta cccatctcaa ataatttata ataacctgct
     3901 aattgatcag caagcgtatc gttaacggtt tctgtttgtg tttgtttcat ctttctcttc
     3961 tcctgtttca attaatctaa aatttcagat tgagcaagca actcattgtg gtagtcggtt
     4021 tgaaattcaa ataacaattt tgccttctta acgaggttta aagcgttcgt tagtgattca
     4081 cttaatgctt caacatcttt aactgatttt gcatgtttca ttttttcagt gtgcgtatag
     4141 cagaattcag gtattgagtt aggggttgcc tgttcaataa acatctcaat tcgcaagtta
     4201 ttatcctcaa aaataattag atcattggca aagtgtttgg tactaacgct aatgccagtt
     4261 gtatctttta atagattatt gattaacgtt cgagagaaat agtttaagtg tgtagatttc
     4321 ataggttcgg tctccttttt acttttcaag caactcttct tctagtgctt tttgaagtag
     4381 ttgagagaag tttatgtttt tctccttccc taaaagaact aaccattccg gaacactaac
     4441 attttttcta attgtttttg tatttaagct tcttcggaaa agtttaatgt ttagttggat
     4501 gattactacc acttggttgg ctttcaaagt taattcttcc actgctgatg gtttaggaaa
     4561 ttctgcatct ggtacactat atagcgctaa tcctaaagca tccttagcgt ttaaatatgc
     4621 ttcttcaaga ctatttccaa aggtagatat ttccggtaag tcagggaaaa cgatgttata
     4681 agcttctcct tctttctcga agattgccgg ataaaaaata ttttttgtct tcatttcctc
     4741 gctccttttc tagtgtaaga gaggatagta tgcctaatac caaataggaa ggcaaggctg
     4801 ttaaagccct gcttgcttcc agatattagc ttctgttcct agtggtacat caccattgtg
     4861 gatcggaaca acgaccgttc ttttagtctt ggaattataa aattttgcat gactgccagt
     4921 ttgtccagtc tttttgaagc cgttagattt gagaattttt acaatttctc ttgctgtctt
     4981 aggcatagct accctccctt tacaaaatct attatacaca ttgttgtgta tgtaatcaat
     5041 agaaaaagag gatgttttat tgcaaaacta ataaaatgat ttattggttt tttcagcaaa
     5101 ttgaatgtta ttagcgttgc aaatattcat aatttcccaa gtatcattag aagtataggt
     5161 atctaagaat tcaatgacag ttacggttga ttcgtatggt tcataagcca aagccttctc
     5221 caagtctttt gttgaaatga tcaagacttt ttcaatttcc tccccttctt catttgtgaa
     5281 aacgtattcc aaagtgattt gttcttcatc ggtataattc atattgtgtt ctcctttctt
     5341 gtattaagaa aaatggatat ttccaattgc atcccactcg gcaccattgt ggtataagac
     5401 gtgaccgagc gttacgcctt tgaaatgctc ttctaaatac atccaaacgc attcaaggct
     5461 agtacctgct ttgagattgt agaagtctgc atccaatacc ttttcagaag ttatcggatc
     5521 aatgcgagtt ggtacctcaa taaattcagt ccaaatttct gcaaaagtct taccttccaa
     5581 agtccacact ccctatcaat taattccaaa agcaaataga gaaatcaagc gaccgtcaat
     5641 agtcacttca acaactgaaa atgccgttga aacagtttta acatccacac tttccaattt
     5701 cccgatgaag gaatttttgt gctttagaat agctttcttt aactgtctgt tatacgcttg
     5761 acgactgtta aactttttga attctcgatc cttgatcagc tgtttaacga tccctgcatt
     5821 aattgtatag ctttctgcaa tagcaacgtg agtaggttgc tgtaatgtag ctgttagcat
     5881 attgtcactc cttcctaaaa gttaattagc ttgtttacaa ttacattata cacactagtg
     5941 tgtataatgt aaagagaggt tttttgctga tggatcagct gtgctaaaca aaagaaaacg
     6001 atagagtaac aaaaaaagtc agcaaagtta aaaaggatga aacggctagt ccggttgctt
     6061 tgcttgtgta agtttcggct ttcttataga tcaaatagga aattataagt gatattccag
     6121 taaagaataa tgtactagct gtcaccaaaa gaaataggtt aatcatacga tacactcctt
     6181 ctataatatt ggtatcagat acgtttaaat gcgtttctga gctgttttgg atcatatggt
     6241 gatgttttag ctctacccta aaatcaaagc ccagaaaagc atttaaaagc gttcgtacct
     6301 aatacgttta tattcatata catatacata aacataaacg tatacacgtt tatgtttatg
     6361 tatttaagtt ttaggctact tccttaacat ctcctgctga tagtcgagtg aaccgagtga
     6421 gtgatccatc ttgtagtcga aaaataaagc tacgtttgag tttatactga taaacagcga
     6481 catacgatcc atcccagatt gagtaagttt tacctgcaac taataagtca tcaagttttg
     6541 gtttattgct ttcttcttta attctatcta gttgtcgctt aaccattttt tcttgatcac
     6601 cgacaaagaa tttcaaccat tttttcggaa ttttcttaaa gtaagcagga ctgtcattta
     6661 agaagtagat tggtttgtac atttcatctc gaaagatgaa gctgtcaata tcgtaggttc
     6721 cttctgcatc tttgaaaagg ataattccat aggtctttgt tactttggta tctacgattt
     6781 ccaaaccatt tttggcgtac cctttctttt cttcgatcac ttcatttgct gatgaataag
     6841 caccatacca tcccataaaa aatcgctcct tttgtaagtt aattagcttg tttacacttt
     6901 tattatacac accaatgtgt ataataacaa gaggatattg ttctttttta ggtaaagtgg
     6961 gatcgtaaac agtgattttg ttgggttaac aaaaaaacag acaagcctta tcagcttgtc
     7021 tgttacttat aaacttcact tctgtgacca atagacaata taagaattgt cacttcatgc
     7081 tcgctaatat cagctattat acggtatgca cctaccctat atcgccagta gcctgattta
     7141 ttagctgtta gacctttacc atgctttctt ggatcatcag tgcctacaag atttttctcg
     7201 atccagttta ggataacctt tgcctgaaac ttatccattt tttttagcgc tttttgagct
     7261 tcacgttcat atcttacttg gtaactcatc ttataagcct aactctttta gcatatcttc
     7321 gtgggaaata ctttgatcgt tttcctcatg ctctttcatt agttccttgt aggtggcaaa
     7381 atctactaac gtttctgctg tttgcaggga attactacgc ataaattcag aaatgttatc
     7441 tccgttgaat tcagcaactt tttcaagaaa atctttttct tctttagata cacgaatact
     7501 tattactggc atagtttatc aactcctttt caattacatt gtactacgat tgtatgacaa
     7561 atgcaatagt tatatatatg aacatacaca taaacatata catatacatg tttatgttgt
     7621 agaatataaa agcaaatata aatataaaca cgtttatgta tataaacata tacataaacg
     7681 tgtttatagt gtataatgga gttacaaaag gttaataagg gaggtttaat catggaaaag
     7741 aagttgtcaa tcattactgg aaacattgaa aaaggtggaa caggtaaaac aacaacaatc
     7801 tacaatctag gtggatatgc tgtcgaaaat ttaggtatga aggtgctgta tattgatgaa
     7861 gataagtcgc aaaacctatc aaaacgcttt cctgatgctt tcagtaaagc gaaaagagag
     7921 aatacaatca atcatttata tcttcatgga tcagctgaac ctttagctat aaatgagaat
     7981 atggatattt tagtagcagg tgaagattta aaacaagttg agatgcaaat gcgtgatatg
     8041 cctaacaaca gaactctact gtttagatgg atcgttgtta actatgaaca gcttaaagag
     8101 aagtatgatc taattctgat tgatactcat aatgatgcag gtctactagc tcaaaatgct
     8161 tgggctgtat ctgatcttgt cgtaggcatt tctgatccat ctaaagatgg ttttgaagct
     8221 ttgctgaagc ttggtaaaga tattgatgca ctagcaaaac agttgattga tattaagact
     8281 ggtgaaacac tgatggaaac taaatacgct acgatcggta gcaggatcaa acataatact
     8341 aattcaagtc gtgagttcct agaattggta ccaacgttat ctaattatct tggatatgta
     8401 caagagaagg aattgcttaa tgcaactagc ttagaaggta agaatattgc tgagttcgct
     8461 aaggataaga atatctatcg taagaataag gatttctttg aaagcacgtt tacgctatac
     8521 aacaaaataa ttgatacggc acaagaataa ggagtgattg aagtgaaaga aaatagattt
     8581 aaaaaattcg ctgaagctga agtagaagaa gttcctgaag tggttgaagc agttgaagaa
     8641 cctaaacaaa cacctaaaga aacaagaagt tctttggtag ataaactaaa aagtgatagt
     8701 tctgaaaaga agaaaaatta tcaattcacg cttcaaccat cgcaaagaaa gaaactatct
     8761 aaaatcagcg aaaagttagg aattcgatca gattcagctt tacttggcga aatgatagat
     8821 aacttgtatg atgaagtttt tggtgagtga tcagtttaaa gctgatcatt tttttattgg
     8881 aataagcggt taggtatagc ttaactaaat ttcatatacg tgtttatggt tatatacgta
     8941 tacatgttta tatacatgta tacgtatatg tttatgttta tatttgtaca taataaaaag
     9001 gctaggtatt gtaacctcgc ctttttattt ttaaagtaag tctttgttac ttggtgcatt
     9061 gaaccctttt agcattggct tttctttctt tgctgaatga ccggatgtaa ccgttgaagg
     9121 atgctcttgt tgaatagggt tatgatcagt tggtttattc tttacttctg ctactttgct
     9181 atcgtttcct gcttcaataa ttgtaacttc attgttcttt acacgccgat ataggttcaa
     9241 agcttttttg atcgtctgag agtatttaac gcctagtgtt tcatattcgg attcaagaaa
     9301 ctctgctaaa ggtaactcgc taaccttttc tctattgatt ataactacat aacgctctga
     9361 ttttccctta ttcatgttga tctccttcgc tgttttattg agttaaacca aacttgtaaa
     9421 agccttttac attagaaaat tcactgtctt caacaaacaa ggtatgcgga attttatctt
     9481 ttacaagttg tttgtcgata atagaagctc caccaccagt aaagacaact ttatctaagg
     9541 ttgtgaagtt tttgaatgta gctaaaactt ctgcaacaac catattggtg tatgttttta
     9601 tttctctttc aactacttct gaaatatcaa aactttcatt tccgcttggc ttaaatagat
     9661 atacgccttt ttctttgcct tctcggataa tcttttcaag ttggaaagca ttaggactga
     9721 tcggaaggtc aagcatattt acgatacgat tatataaagt aaaaatgccg gtatcctttt
     9781 gttcacgatt gttttcgtca atttcaaaat tttggacaac atcaaaaagt ttcgtgccac
     9841 cgccaatatc aacaaaaccg atcttctgac tgatcaatgt ttcatcttgt acactaccat
     9901 catcagcaag taggaggtta tatagtgttc caatcggctg tggcagtacc caaacggtat
     9961 ctacgtttat attataagcc ttaccgtcaa cttcaactga atgttgtcgt gtggcttgtt
    10021 gcatcacgta tttgacgatt gtttcggtat agtcggatga aggcagacct aaaactagat
    10081 taacggttaa aacgtcatca gattgatctt ctaaacgtgc taggttagct aaagagaaag
    10141 ataggatatt ttggtaggtt tcctgcttgt atctgctgat cccaaaacct agtgattcta
    10201 tcacgtagtc atcttttcct agctcgtgta tatccttccc aaagtaatag ctttcagtag
    10261 tgtttactag gctatattca gaaacgttta gtgatcgttt taatgaggaa cctatattag
    10321 aaaaacgtcc tgaattaatg atatttttgt tcaaaatacg agagggaagt gagagtggag
    10381 aaacgtgtgt actcatttct tttactgatt tattccctaa gtcaacggaa aacaataatg
    10441 gttctttcat ggtatgacct ctttctgttt gtgtttatgg tatttcggta cgaaaatagt
    10501 ttacacctat ttactattag ttgcaactgt ttataagaaa aaaatgcaat cataaaagta
    10561 taattaatta taataaagtg taaataaaaa ggtgtaaata aatgcttgca aatttatctt
    10621 attatgatat tataaaaaca taaattatat taaggagtga tcctgatgcg attgagaaga
    10681 aataagaatt acactaccat tattttagaa gattttactg atcaagaatt acttattgtc
    10741 ggtaaaacaa aagcagattt tatcgaaaag ggaaatgctt caatagtaaa agcttcaaag
    10801 gttgctgtcg aaaaatattt tgattcttta gaagaaagtg ctaaggaaat ttttaatgga
    10861 gagaaagctg gtagctatga gttagacggt attagattta gcttgatccc aacaactgaa
    10921 aatattattc cgaagaaatt atattatatc ttttcatctg aaaaatatac ttcaatgaag
    10981 ccttgtattt tggattggca atggttgatt gatgctgtat tggatgtaaa atgttcaaaa
    11041 tacaatatta ctttggggtg ttaattatga gtatgctagt tagaaaatta ccattatctg
    11101 taagagaatt tctattttac ttggtctgct taatcgcatc agggtttaag ctgatcgaac
    11161 atatttttat ctttgcttat gtattagcaa tgatcgcatt tttatctggc ggttggttaa
    11221 ttatttattt gttaagctaa gttttagatc aaaaataatt aataaattaa acgataaaat
    11281 aaccgttaat aaatgtaatg aaagggcaac gcctaacaat cagcttgtaa taagattata
    11341 aaataacata atatcaaaaa agtgcttttt aaaggacaa
//
LOCUS       DACDIE010000079        10509 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.079, whole genome shotgun sequence.
ACCESSION   DACDIE010000079 DACDIE010000000
VERSION     DACDIE010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.079"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(3..1847)
                     /locus_tag="IV250_002406"
     CDS             complement(3..1847)
                     /locus_tag="IV250_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769652.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
     gene            2059..2577
                     /locus_tag="IV250_002407"
     CDS             2059..2577
                     /locus_tag="IV250_002407"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP8769653.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            2705..3388
                     /locus_tag="IV250_002408"
     CDS             2705..3388
                     /locus_tag="IV250_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAP8769654.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            3697..3770
                     /locus_tag="IV250_002409"
     tRNA            3697..3770
                     /locus_tag="IV250_002409"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3731..3733,aa:Arg,seq:tct)
     gene            3929..4288
                     /locus_tag="IV250_002410"
     CDS             3929..4288
                     /locus_tag="IV250_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HAP8769655.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            4411..6042
                     /locus_tag="IV250_002411"
     CDS             4411..6042
                     /locus_tag="IV250_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAP8769656.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(6104..6745)
                     /locus_tag="IV250_002412"
     CDS             complement(6104..6745)
                     /locus_tag="IV250_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP8769657.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            7027..7335
                     /gene="rplU"
                     /locus_tag="IV250_002413"
     CDS             7027..7335
                     /gene="rplU"
                     /locus_tag="IV250_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAP8769658.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            7362..7706
                     /locus_tag="IV250_002414"
     CDS             7362..7706
                     /locus_tag="IV250_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAP8769659.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            7719..8012
                     /gene="rpmA"
                     /locus_tag="IV250_002415"
     CDS             7719..8012
                     /gene="rpmA"
                     /locus_tag="IV250_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAP8769660.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            complement(8105..9253)
                     /locus_tag="IV250_002416"
     CDS             complement(8105..9253)
                     /locus_tag="IV250_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8769661.1"
                     /translation="MAMIKQYKKKNGEKAWYFKTYLGIDPLTGKKKYTTKRGFRTQKE
                     AKTALSRLELELQKTGMPTSTNTTFKEAAELWLESYKKTVKESSYSRTKIIFNKHIYP
                     KFGNIKLSKINTAYCQKVVNDWSEKGTSKQYPLFINYMNKVFKYAINIGLTSDNPTLN
                     LLIPKPQIKTEKKLKLYTKEQLELFLNEVSQEQNPYFKNRDYTLFRLLAFSGCRIGEI
                     LALTWDNINFKTNEMAIKKTVARSDKYYISETPKTKKSNRIIYLDEKTIKQLKFWKLE
                     QRKYLFQLGFTKANYLFTNDENNFTINQSVAERYNIYRERAGLPYIGLHGFRHTHASM
                     LYEAGADHKEVQERMGHANIKTTMDTYTHITNSKKEETTQKLTNYINF"
     gene            complement(9265..9576)
                     /locus_tag="IV250_002417"
     CDS             complement(9265..9576)
                     /locus_tag="IV250_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769662.1"
                     /translation="MIYTEFKEWLEKSTTGYETFIIKATNYQIEKNKNRPQKKRWDDK
                     KIDKAVLEMWKQVVTNLYQTIRKEKGVPLINGKEIWLEFIEEQGLIEFFNDSMAELEF
                     E"
     gene            complement(9629..10024)
                     /locus_tag="IV250_002418"
     CDS             complement(9629..10024)
                     /locus_tag="IV250_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP8769663.1"
                     /translation="MIINELGVKVEERENLDADGHYVACMNTIVIKANLSKYRRQRTL
                     LHELGHASKHHDNYFLYNLAFSLHSKMEYEADRFMIEKLLDRYIAKSELEPHNINYMK
                     FIEDNNLSVRFEPLVKELLKARIYCYAAL"
     gene            complement(10060..10404)
                     /locus_tag="IV250_002419"
     CDS             complement(10060..10404)
                     /locus_tag="IV250_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8769664.1"
                     /translation="MLFERVKELCKKRGISISELENRIGFGKNSIYKWKTQSPKIETL
                     QKVADYFHVSTDYLLGRTDDPNAGVAPEERKLTVEEALASVMSSDGKPLTDNDREILS
                     AMIEAYLEKKDN"
BASE COUNT         3216 a         1733 c         2009 g         3551 t
ORIGIN      
        1 ttttatacag gattcgggta atttcgagag agttgaacag tgatccgatc aatgaaagta
       61 tctttttcct gcgttagtgt tcctgtgtaa cgatccgcta cgtctttcat catcattaag
      121 tcaaaacgtt cctgatcatc taattcttct gtgatgcctc gactaaatag gtttgtatcg
      181 taatgtgcac tatgttcgca acaaatgacg aaatgttcct cttgttcgtg tatcgacaaa
      241 taaatttttc gatacagtgg attttcgatg gcatgagttt ctctaaagga atgctctaaa
      301 gcgtggatca aaagttgagc cagatcgtca tcgttgatag gaagttcttc gggaagatct
      361 gcttggaagg ttatctgaat attccgtttg gcagctaact tctggtaacg cgctaagata
      421 agatttgtca cctggtggtt gcaaaaagaa cacagcattt cgaaattttt tgcttcttct
      481 aatatctttt ccacatagtt tttagctttc gtttggtccg tatgcattaa atcttgcaga
      541 ttctccatct cttggacaaa ttccaggcga agtgtattta cttcttggat atgctgtcga
      601 agctgctcat aatgttgctc caacaaatca tttttgattt ttaagaaatc tacttgtctc
      661 cgatctttgt cggtatttaa gatgtgatcg attagattgt agctgataat aacgattaga
      721 atgataatat ataaaatcgt agaccaattg atcgttgttt gatagatacc taatgccgtt
      781 tggatgtaaa tcaagcaatg gataatggct accaagacga tccaaggtgt acaagctaca
      841 taaaaacggt tattcttata tgcttcgcca atgcatgcaa gtgaagtgac taaagtgctt
      901 ataacactag caatattagc aatcggcatt aattcaggaa gttctgcagg agtaaaagtt
      961 gcataaagca gaacgccgat aaaaatcaaa agttggaacg tacaaaaaat tccgtaccat
     1021 tttttatagt aaaacattcg gctccaatag tagcagatga tgaaagctga cgttaagata
     1081 ataaataaat gagataaaaa agaatgaacg aatggttcaa aaagaatacc acttaaaata
     1141 tcttctgata cagcttcaaa tcctaatgcc aaagcaaagc aacttaagag aataagtgag
     1201 tattcaagac gtttttgttt atacatcaag tagatcgtat agaaaaaggt ccctatagcg
     1261 ataaaaacag cgattatcat cgtcaatact tgcggcactg attgtgaata aataaaacta
     1321 atcaatggtc cagttgttcc tagataaact ttcggtggca gtccagcata gttctgataa
     1381 ggagatgaga ctgtaagggt caattttttt ccgatataat ttgctggtaa atctatcaca
     1441 gccaatgaga gtccgggatt gctttcttcc tgataatctt tccgttggta taaaatcttt
     1501 tcgcctagat atacagtaat ccattgatgg tttcctttga ttagtaaatt tgcttcgtcc
     1561 aacggttttt caagtgtcgt ttccataatg aatgtttcgt ctttatccac ggtaggaatc
     1621 gtacgagtgt acttgctgtc aaacttaaaa tcaggatcat cttttgtata gaaaacccac
     1681 gaatctttta tttctttcat ttgattgctg tccgcaggag aaaaaataaa ataaaagaaa
     1741 taagcaattc ctgttaaaaa taacaatgat aatataatga ttatgctcca ctttatacgt
     1801 aaataaaaag attctttttt attatatatg ttattaaaaa aagacataaa ggtattcctt
     1861 tctaaaactt gtaatgttag ggtaaacgtt ttttaaacta acgtcaatta tttataataa
     1921 aaaatgagat aaagatgagg aaatagaatt agaaacatat aattattaaa gggaaacatt
     1981 tttacagcta taatttgaaa aaaatttatc gttattatat gattaagaag gattgaataa
     2041 agaaagaagt tgaaaacgat acctgattac aaaaaaataa cttttataag ttctataacg
     2101 ttgattatat ttttaattct tattcttctc gtcgtcttgg ttccccgaat ttttggtttg
     2161 actccttata ttattgagga caaagaaatg agtccatatt atgcacaagg aaatctgata
     2221 tatgtacgac cgaaaaatac aaatgagatt ttagtcggag atattattac atattatgaa
     2281 aattcaggaa aaaaaatctc gacgcgtcgc gtaatagccg ttgaaaacaa ccacgaagat
     2341 ttttatacaa aagctgatac aaagacttat attgagcctg gagtagtaaa aaagagaaat
     2401 attatcggta gtcccatctt tcagattcca tatattggac tagtcctttc aaaaacggca
     2461 tttgcatgga ttcaggggtt attctttacg attgcttttt gcctaacagg aatcacagca
     2521 tggaatacct ttgtagagtt gaaaaagaag cggagacgaa cacagaaatt tttgtaagaa
     2581 tgttaaaaac ataaatgtat tccagagatc caacgactct tctgaacatg aaacacgaga
     2641 aaacactcat gcagcttgta gatttagtca gttgttcgta taatgtgtga agaggaggag
     2701 tagtatgcgc aatgaaatga tgcatcgtcc tgtagtcaaa gaagaactat tggattttat
     2761 gcgtacccag caaaaaaaat taccgggtga gctaggcaaa ctggaagaag aagctcatgt
     2821 agcagaagta ccagttattc cccatgaaac agttgtattt ttgaaatttt tacttggaca
     2881 gcttcagcct gaacgaatct tggagatcgg agcagctatc ggtttttcat caagtgtcat
     2941 ggccacgaca atcggagaaa acgctcatgt tacgacaatt gatcgtttcg atgtcatgat
     3001 cgagaaagct aaaaaaaact atgaacgtct aggactgaca gataaagtga ctttgcttga
     3061 aggtcaagca gcagatatct tgccggagtt gtctggtccg tatgacttta tttttatgga
     3121 tagtgcaaaa tcaaaatata ttgaattttt acctgaatgc ttacgtttgt taagaaaagg
     3181 tggcgttttg atggtagatg acatcttcca aggaggaacg atcttgctcc cagatgaaga
     3241 gattccccgt ggcaaacgag ctatccatcg taaattaaat gaatttcttc gtgttgtgat
     3301 ggatcatccc gatttgacat cgacaatctt gccattaggt gatggtgtga tcttaatgac
     3361 aaaggaatca gaaacaatcg agttgtgata catgattatg gcagcctgta gataatcgat
     3421 tgaatcgagg gctcaattcg aacttgatgt aaaagtttct atatttgtaa gtcaattaaa
     3481 ggatacagga aatctaaata aacaggagta gcatgaagtc aatgtagctc ctgttttcaa
     3541 ttgataacct tagaacagct gaaggagaat gtctcgtttt aaaagattga tttagtagtt
     3601 gggccaaaat aattcattat ttgctaatta aataactatt tcgtattgac agaagcaatt
     3661 taattatcta taattattga tgttccgctg attcttgtca tagtagctca gctggataga
     3721 gcatccgcct tctaagcgga cggtcggggg ttcgaatccc tcctgtgacg ctaaaaaacc
     3781 gttagaaact gaggtttcta acggtttttc tttgtttttt gtgccaatat tgtttgtttt
     3841 caaaggcaaa taaaataaaa aaacatcctt atcgagtaca attataagga gcaaaaaaat
     3901 aaaactcatc agaaaagagg aagaagatat gacgatacta tctagaattt tagtgactct
     3961 agttgcaatt gaattttttt atatcatgta tatcgaaacg gtgagaactg attctgacac
     4021 gacaagtagg gtttttaaaa tgagtaaaga agagttaagc agaaaatctg ttcaaacatt
     4081 gtttaagaac caaggagtgt acaatggact attaggagtg ggactacttt atggagctta
     4141 tctttcttcc gcatctaaag aaatcacaag tatgctatta atatctattt tttttgttgc
     4201 attatatggc agtttgacta gcgatcgttg gattttactc aaacaaggtg ggctagctct
     4261 gttggcattg ctttctattt tcttataaat atagaaaaaa taagaattta tctaacagga
     4321 aactttgtga aaaatattac tttatctcat gaaatatgat attctaatga atggtatttt
     4381 agaaaatttc atgaaaaagg agttagatag gtgttcaaaa attatgtttc ctcactaaaa
     4441 aaagaatttt caggttatga tctttcaaga tttcagaaag atcttttggc tgggatcaca
     4501 gtagctgctg tagcattgcc tttagccttg gcttttgggg tatcaagcgg agctgatgcg
     4561 gccgcaggtt tgattacggc tatcattgcc ggtatcgtta ttggcggttt atcaggcggt
     4621 ttttatcaaa tatcaggtcc gacaggtgcg atggcagcta tcttgatgtc tattgcggca
     4681 acaaagggaa tgcaaggtgt attgcttgct acttttttag cgggtctatt tttacttgca
     4741 gcaggacttt tccgattagg aacactgact tcttttatcc cagcacccgt aattacgggg
     4801 tttacttcag gtattgctat cattattgca ttaggtcaga tcgataattt atttggtgtt
     4861 cactcggaag gtacaaacgt tattgaaaaa ctaagtagtt atcaaacgat aggatttccg
     4921 ataaacacag cctctttgat catgggaggg ctagtcattt tagggatgtt tatctatcca
     4981 aaaaaatggg cgaaacgaat gcctagttcg ctgctggcta tcattttggc aacagcagtt
     5041 atgctattgt tccatttacc agtagcaact gtgggtaaga ttccacaaac attgatcagt
     5101 agtaatcgtt tgaatatggg agattttagc ttttctgcct tgcaacaagt ggcagttcca
     5161 gctattagca ttgctttatt aggaatgatt gaaagcttgc tatgtggtgc ttcggctggc
     5221 cgaatggcaa ataagccctt agatagcaat caagaattaa tggcgcaagg aattggaaat
     5281 ctgttgcttc ctttctttgg cgggattcct gctactgcgg ccatcgcccg tacaagcgtg
     5341 gccatcaaat ctggtgcaca aactagatta gctggtatga tacatgcagt tgtcttactg
     5401 ctctccatgc ttgtttttgc gccagtgatg tcacagattc caatgccagc acttgcaggt
     5461 gttttgattg tgacggcttg gcgtatgaat gaatgggaaa cgatcaagga gctatttact
     5521 aataaatatt ggtcagcagt tctattgttt atccttacga tgatttgtac agttatcttt
     5581 gatttaagta ttgctatcgt gatcggggtg atcggcggct gtgtattttt tgttgtcaaa
     5641 agcgctgcta tcacgatttc attagaaacg atcgattggc aaagaatggg gctgccagaa
     5701 acggaaaagc tgaataactg gacagtagtg tatattagtg gtccactgtt ttttatgtct
     5761 tcagaaaaac tcaaaaataa attggaagaa ctaaatcatc acgatggtgt cattttctcc
     5821 atgcgaggcg taccaagtat tgatttgacg gcacgtgcag tttttgatga atttcaagaa
     5881 aaagcagagc aaagaggaca aacgattatc tatacttcct tgcagccaga agtagaaaaa
     5941 caactttctc acttgtggga acatacattc acgcatcaac atcaaacagt tgctcatgcg
     6001 ttgacagctt tacatgcgaa atatactgca attagccatt aaaacaatga aaaagaacag
     6061 acaagactgt aaactgaaga ttttatggtc ttgtctgttc tttttaatat tgctgtaaaa
     6121 gtgaagctag tacttgccag tcttgatgga taacaggtac aagggagcga ttgtagaaaa
     6181 tagctgctgg atgaaaaagt ggaagaattc gatagttttt ttctgaccat tcgtaggcat
     6241 ccttttgttc tgttaattgt aagacaggcc ctgtatagag ttgaccatga tttttggtaa
     6301 ccgtatattc tttgccaagt aaacgttgaa gaccaatgtt tccaagtgtc gcaattaatt
     6361 ttggctggac cttcatcagt tcgtaatcca atattggtgc atgcgcaaat acttcggaac
     6421 gagtaggtgt ccgatttgga taaacagttt ctgactgttg tgtccgttta ttgatccgat
     6481 gagtcacgcg atacgggcgg ctccttacag cactggtgat atacacctct tctcgtgtta
     6541 agccagttga agaaagtgcc tgcatcaatt cttttccagc ttgaccgcta aaaggaacat
     6601 gcgtatcaat ctctgttttc cctggggctt ctcccacgag catcagttta ggatgaagag
     6661 gtccttgccc agcgataaat ccttctacag gacggccgtt cattcttaaa gaagcttctt
     6721 gtatcagttc ttttgggtat tccatttatg ctcctccttt ttcttttatt ataagaataa
     6781 aagaaacaca atgcgactat tttatttggt agaactgtga ggatagaaaa gcagaagtat
     6841 tgcttgaatt tttgttttac tttttgattt taaatttagt tgactaacgg ttgctgttct
     6901 ggtaagctag tactgttgta attgtggact ccacagctac aaccgctcag aacaagtaat
     6961 aagcacagca tatgctgtca cttcggatgg cgagtctaag tatatgagaa ggaggtgctt
     7021 tatagcatgt acgcaattat taaaactggt ggtaaacaag taaaagttga agtgggacaa
     7081 gcaatctatg ttgaaaaact tgacgttgaa gcaggagaaa aagttgtttt tgacgaagtt
     7141 atcttagtag gcggagaatc tacaaaagta ggcgctccaa ctgttgcagg tgcaactgtt
     7201 gaaggtactg ttgaaaaaca cggcaaacaa aagaaagtcg ttactttcaa atacaaacct
     7261 aaaaaacatt cacaccgtaa acaaggtcat cgtcaaccat atacaaaagt ggttatcgac
     7321 gcgatcaacg cttaatagcg tttgagaaag aaggcctata tatgatcaaa ggaacattta
     7381 agcgaaatga cgcaggtcag atcgtttcat tcactttgac cgggcatgct gatgctggtc
     7441 cttatggaag tgatatcgtt tgtgcgggtg tttctgcgtt agcgatcagc actgtcaacg
     7501 ggattgcctc attagccggt tttgagccga ttgttgaaat gaatgaagaa gaaggcggtt
     7561 atctttatac cgaagtcact tcaggaatga cacaagaaca aaacaatatt gctgagattc
     7621 tattagaaaa tcttttatta ggattacaat cgatagaagc tgaaaactca gaatatatcc
     7681 aaattaaaac aattaatgat aaataggagg tgcagaccat gttgttatct atgaatctac
     7741 aattatttgc ccacaaaaaa ggtggcggtt ctacatctaa cggccgtgac tcagaatcaa
     7801 aacgtctagg tgctaaacgt gctgatggac aaacagttac aggtggatca attctttacc
     7861 gtcaacgcgg tacaaaaatc tatccaggtg taaacgtggg tatcggtgga gacgacactt
     7921 tatttgcaaa agttgatggc gtagtacgtt tcgaacgtaa aggccgtaac aaaaaacaag
     7981 tgtctgttta tccagtagct caagaagctt aataaataaa ctgctctatc tcttgtgtag
     8041 caagggatgg agcttttttg taaattatgg gatttttttt gaccatactt ttgaccatac
     8101 tttcttagaa gttaatataa tttgttagtt tttgtgttgt ttcttctttt ttgctgttag
     8161 taatgtgtgt atatgtgtcc atagtagttt ttatatttgc gtggcccatt ctttcttgga
     8221 cttctttgtg atctgcgcct gcctcatata gcattgatgc atgtgtatgt ctaaaaccat
     8281 gcagaccgat ataaggtaag ccggcacgct cacgatatat attgtatctt tctgccactg
     8341 actgattaat tgtgaaatta ttttcgtcat tggtaaacaa ataattagct ttagtaaatc
     8401 ctaattgaaa taagtacttt ctttgttcga gcttccaaaa ttttagttgc tttatagttt
     8461 tttcatctaa ataaattatt cgatttgatt ttttggtttt aggagtttca gatatataat
     8521 atttatctga acgagctaca gtttttttaa tggccatttc atttgtttta aaattaatat
     8581 tgtcccaagt gagtgctaat atttcgccga ttctacatcc gctgaatgct aatagtctaa
     8641 agagcgtata gtctctgttt ttaaaatatg gattctgttc ttgagatact tcatttagaa
     8701 ataattccaa ctgttctttt gtatataatt ttaatttctt ttctgtttta atttgtggct
     8761 ttggaataag taaatttaat gttggattat cagatgttaa accaatattt atagcatact
     8821 taaaaacttt attcatatag tttatgaaaa gagggtactg ctttgaagtt cctttttcac
     8881 tccaatcatt tactaccttt tgacaatatg ccgtattaat tttagaaagc ttaatatttc
     8941 caaatttggg atatatatgt ttattaaaga ttattttagt ccttgaatat gaactttctt
     9001 ttacagtttt tttgtagctt tctagccata attctgctgc ttctttgaaa gtagtatttg
     9061 tacttgtggg cattcctgtt ttttgtaatt ctagttctaa cctagaaagt gctgtttttg
     9121 cttctttttg tgttctaaat cctcgtttag tagtatattt tttctttcca gttagcggat
     9181 caataccgag ataagtttta aagtaccatg ctttttcgcc atttttcttt ttatattgtt
     9241 ttatcattgc catcaatatt acccctattc aaattctaat tctgccatgc tatcattgaa
     9301 aaattcgatc agtccttgtt cctctataaa ttcaagccat atttccttcc cgttaattaa
     9361 tggaactcct ttttctttac gaattgtttg atacaagtta gtcacgactt gtttccacat
     9421 ttctaataca gctttatcta ttttcttatc atcccagcgt tttttttggg gtctattttt
     9481 gtttttttca atttgataat tagtagcttt gatgataaat gtttcgtatc cggttgtgct
     9541 tttttctaac cattctttga attctgtata tatcattttt gttcaccttt ctatttttga
     9601 acgtatgttc ttttttgctt aaaaaatttt agagagctgc ataacaatag atgcgagctt
     9661 ttaataattc tttcacaagt ggttcgaagc gaacgcttaa attgttatct tctataaatt
     9721 tcatgtaatt gatattgtgt ggttctaatt cagactttgc aatatatcta tctaataatt
     9781 tttcaatcat gaagcgatca gcctcatatt ccatttttga atggagagag aatgctaagt
     9841 tatataaaaa ataattatca tgatgtttag aagcgtgtcc taattcatgt aataaggttc
     9901 tttgtcttct atacttagat aaattagctt ttattactat ggtattcata caagcaacat
     9961 aatggccatc ggcatcaagg ttttcacgct cttctacctt aacgccgagt tcattaatta
    10021 tcatttcaat ttgactgtcc aaatgactca cctacttatt tagttatctt ttttctctaa
    10081 atatgcttca atcatagctg atagtatttc cctatcatta tcggtgagcg gttttccgtc
    10141 gctactcata acagatgcta aagcttcttc cacggttagt tttctttcct ctggtgcaac
    10201 tcccgcgtta ggatcatctg tgcgtcctag caaataatct gttgatacat ggaagtagtc
    10261 agcaactttt tgcaaagttt ctattttagg actttgtgtc ttccatttat aaatagagtt
    10321 ttttccaaat cctattctat tttctaattc agaaatagaa atacctcttt ttttacagag
    10381 ctctttaacc ctttcgaaaa gcatgtcaaa tcaaccttcc tagagcgtac gataataatt
    10441 tatacaaaaa gatagaaagg tagttgacaa atctatccaa ttgtcttata tttaactcgt
    10501 aagctaatt
//
LOCUS       DACDIE010000080        10358 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.080, whole genome shotgun sequence.
ACCESSION   DACDIE010000080 DACDIE010000000
VERSION     DACDIE010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10358
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.080"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            14..160
                     /locus_tag="IV250_002420"
     CDS             14..160
                     /locus_tag="IV250_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HAP8769665.1"
                     /translation="MLVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEENRKKAMRK
                     RVSR"
     gene            complement(222..2033)
                     /locus_tag="IV250_002421"
     CDS             complement(222..2033)
                     /locus_tag="IV250_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAP8769666.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(2030..2395)
                     /locus_tag="IV250_002422"
     CDS             complement(2030..2395)
                     /locus_tag="IV250_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAP8769667.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(2558..2953)
                     /locus_tag="IV250_002423"
     CDS             complement(2558..2953)
                     /locus_tag="IV250_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP8769668.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(2971..3933)
                     /locus_tag="IV250_002424"
     CDS             complement(2971..3933)
                     /locus_tag="IV250_002424"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HAP8769669.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(3933..4145)
                     /locus_tag="IV250_002425"
     CDS             complement(3933..4145)
                     /locus_tag="IV250_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HAP8769670.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(4166..4864)
                     /locus_tag="IV250_002426"
     CDS             complement(4166..4864)
                     /locus_tag="IV250_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAP8769671.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(4884..5426)
                     /locus_tag="IV250_002427"
     CDS             complement(4884..5426)
                     /locus_tag="IV250_002427"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAP8769672.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(5558..6562)
                     /locus_tag="IV250_002428"
     CDS             complement(5558..6562)
                     /locus_tag="IV250_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP8769673.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(6559..7548)
                     /locus_tag="IV250_002429"
     CDS             complement(6559..7548)
                     /locus_tag="IV250_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP8769674.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(7545..8351)
                     /locus_tag="IV250_002430"
     CDS             complement(7545..8351)
                     /locus_tag="IV250_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8769675.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            8517..9473
                     /locus_tag="IV250_002431"
     CDS             8517..9473
                     /locus_tag="IV250_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8769676.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(9550..10068)
                     /locus_tag="IV250_002432"
     CDS             complement(9550..10068)
                     /locus_tag="IV250_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP8769677.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(10156..10305)
                     /gene="rpmG"
                     /locus_tag="IV250_002433"
     CDS             complement(10156..10305)
                     /gene="rpmG"
                     /locus_tag="IV250_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP8769678.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
BASE COUNT         3215 a         2275 c         1774 g         3094 t
ORIGIN      
        1 atttgtatct ggaatattag taggaagtct ggcaacagcc gctgccgttg ctggtttggt
       61 tgcttctgtg aaaaaaacag tgatcgatcc aattgacgaa aaagaagcaa tgatcgaaga
      121 aaaccgtaaa aaagcaatgc gtaaacgcgt ttctcgttaa taattattta aaaatacgca
      181 tgaagacttt ctaaaaaaca gataagtcat catgcgtatt tttatttgtc agtcaatata
      241 agttgtgcta ttttatctgc ctgtctcaga ttccatggat ctgtataagg tctagctact
      301 gctgcagtta gccaatccac accttcaaca ggtattccat agcaataaaa gtcagactgg
      361 atttcccctg aagcatcaat cacgcgattc gtacctcgct caacatctat agcttctgaa
      421 tgataacttc ctgtattatc tgaaaatgaa aaagggcgaa caagtttgtt ttccttcaaa
      481 ttttttatga caggattact tgaacgagaa aagcttgttt gtggcaatcg agcttcaatc
      541 aaatagcgac tgctgatttc tttatcttgt agagaaataa catagcggtt gtgcttttgc
      601 tgaatatcaa atggcggttc gatcaaatga aaaatacccg catctatcag tgctgctaat
      661 tctttcgtcc gaatgattgg cggaccgatc gttaaaaagc tatttagatg agtaaaccat
      721 ccccatagta tttctttgta ttccttagca gaaaaaacgt gccattcaat agcctgccgg
      781 attggatcac gccagtcttt taatgcatca aatgttgaag caaatgctcc tgtgcaattg
      841 cctttttccg cttcttttat ctgcccttca ataaatattc ggctagcttc tgaaaagggc
      901 tcattctgat aggcaagagg agtttctaaa tacgaccaat cccatctata agggattagt
      961 gctggatgct ttccgagaca ctcctctgaa gagagggaga gaaaatcatg ctcaaaattt
     1021 ttctttggaa tcggcaagtg atattcttcg atgattttct tgtaataaaa caactcaacg
     1081 tcttttttca gatagtcaaa aaatgtttcg ccagtcaaaa atcccgactg atgccattgc
     1141 tccaggtttt cttttgttag taaccgtggc catgccattg ctccgccttg cttttgattt
     1201 cttcctcgag ggtaataagg gagccccctt cttgatccac tgtatacaat cggttccttg
     1261 cctgatggat gatagaccag ttttcccttt tcgttctcga agcgccctcc tcttgatact
     1321 gtaaacaatc cgacataatc aaaaaaactt aagcctagac ctcttaaaaa gacattttct
     1381 ttaggcggta tcttcgaaag atctacatct gcaggattag aaggaagctg atacagtaaa
     1441 acatttttct ttgcgtagtc tacccacact ctttcttctt ctgtctcatc attttcccag
     1501 tgaccgcttg ctaaaacaag ctgatcgata gaatagcttt gctgatctgt ctgtaaaaga
     1561 taaccatctt ttcttttgct tacttttttt actaaagaat aaacaaactg aatagaagct
     1621 ggaaattctt cttgcaactt tgagaaaaac cagcgttgat agagtccgta taaacatcga
     1681 gtagattgct gatctttttc cagacgattg atttcttcta taattagttg cttatttttg
     1741 gttaccgtct gttcgatata atctgtagca ggtcctttac tccattgaaa caagttgggt
     1801 ccctgtgtta ccaatcctcc actggacaat gtttcatctg taaaaagcgt cacttgttga
     1861 gaaacagaat tcattaataa ctccggtggc tgattcaacc gccaaactcg acctccagga
     1921 ccattgggat cgaaaagaaa aagttcaaga gaatgtctgt cctgattatt atggagaatc
     1981 ctttctgcta cacttaatcc tctaggtccg gcaccaatga tccctatttt catttgcatg
     2041 cctccatttc gataaatttt ttcaataaaa cagcatggtt ctgatgttca ttttttgcac
     2101 cataaacaaa agtaatgact ggatgttctt tcataagttg actgagttta aaaatagctt
     2161 gcttcttttc ttcttcgaaa atcaattctt gctggtattt ctttgcaaat gaagcaaatc
     2221 gttctggtac atggtcaaaa gttttgcgtg cttcattact aggtgcaatt tctttcaacc
     2281 acagatctaa ctgcgctttt tcttttgaaa ttcctcgtgg ccagaatcta tccacgagta
     2341 ttctatagcc atcctctttt tcagcatcaa ggtaaacacg ttttaatttg atcatagttg
     2401 tttccttctt tctacctttt cgcttagctt atatatgtag ataatagcac aaaataaaaa
     2461 aaatgaacgg cacaactatc cattgcgccg ttcatgtgat cttggtcaaa atctaatgat
     2521 tgattcggtt tttaaagctg tcatacaatc atctgtttta tactttttta cttttgattt
     2581 ttccagacca tccgtcatat ccacccttaa gtaaataaat ctcttgataa cctgctttac
     2641 gtaatttatt tgctgtacgg atgctcaaac ttttcttttg atcgtaaata tagacaggct
     2701 ggtcttttcg aatagatcct aatgaatctt tcaaaacagt gtaagggatg tttcttgcac
     2761 ctaaaatatg cccagcatca aacacatctt tttcgcgaac atcgattact tgtgcttttc
     2821 tcattccttc tcgaaattct tcttctgtca aagtcgttgc tgaacgtttg gccattactc
     2881 gtagatagac ttcccaaagg atgattgcaa gcacaatcaa tagcaaaatg atattaatta
     2941 cccataaaac catgtaatgc ctccgttttt ttaagcaaat ttcaaggcta atgaggcagc
     3001 accaatgaca ccagcttcat taccaagctc tgctaatttg atttttgttg attctcttac
     3061 ttgcgggaaa gtaaattctt taaaatattt ctctacacgg ctacgtaaaa attctcctgc
     3121 tgccgatact cctccaccta acacgatact tgacggattt aatgtattcc ccaagtttcc
     3181 gcaagctaaa cctaagtaga agcatacgcg atcaacaacc atcaatgcaa acggatcgtc
     3241 ttcttgagcc atttcaaaga tatctttact tgttacttct tctccgttgt ctagcattgc
     3301 tttcagtttt gaatcccctg cgtattcttc tgccaagaaa cgacctaggc gtaccacacc
     3361 tgtagcgcta gatacagttt ctaaacatcc gcgtttaccg caagtacatt cgaacccttc
     3421 aggatccaca gtgatatgtc cgatttctcc tccagctccg ctcacgccgt gaatcaaatt
     3481 tccgccagca acgatacctc cgccaacacc tgttcctaga gtaacgaata caacgtctgg
     3541 atcgttttct ccagcacctt tccatcgttc acctaaagca gcaacattcg cgtcattatc
     3601 gatagcgaat ttgattcctg ttcctgcctc gatcagtttt ttcacaaatt gaacttctgt
     3661 ccagtttaag ttatacgcac caataaccgt tccagcttcg ctgtccacgc ttcctggggt
     3721 acccatgccg attccgataa agtcttcggc tttcatatta tataaattca aacgatgatt
     3781 gattgattca atgatttcag gaacgatatg cttaccatca tccaaaatat tggtatcgat
     3841 gctccatttt tgctgaatct ctccttctgg tgttaaaatt gcaaattttg ctgttgttcc
     3901 acctaaatca atcccaataa tcttcttgtc cattacaaat ttcctttctt tcggctttct
     3961 tcaattcggt gctctctttt taatatgcgc cacgctgtca tgtatgtatc atgatcaatc
     4021 aaacggctat catacagatt acgcaattcg atagtcatta attcaatgtc atagatgcgt
     4081 gcacccacat agataaagat gccaaatttt ttgaacagct gctgtacatc atataagctc
     4141 tccatcttta agcacttcct tcatattata caagtaaacc atattttttt aaacccagta
     4201 aaatacatat tacaaaaaga aatacaaaaa ctagtgtaga aagaatcctt tggtgtatcg
     4261 aatagcttgt ctgttgtttc ggtagaccca aaacattccc taacactaat ccgccaatta
     4321 gcccaccgac atgtccccaa acatctacag aggtatccag aagaccaaaa acaaatgtca
     4381 acacaataaa taaaagaaaa tgtctgacta gctgctggat cgctgggtta tgtttaaaat
     4441 gaaaccctaa gataaacaac gcaccgaaca aaccgaatat cgaagtactt gcaccaccag
     4501 acagaacatt cgcttcgtta aaagcaaagc ttgctgtatt tcccattatc ccgctgaaca
     4561 tataaatcaa gaaaaaacgc caatggccat agattgcctc gatttgctgc cccataaaat
     4621 acagtatcag tgagttcatt acgaaatgca tcagtccaaa atggatgaag attggtgtca
     4681 ctaaacgcca ccattcattg aagtgaacaa gataaggacc atacattccg gcataataag
     4741 ggatctcaag tcgcggaaat aattccatca tgaggaagac cgctgtctgt atccctaaaa
     4801 aagcataggt ccataacggt cgtcttaacc atgcacgcaa ttttatttgc tgttgataat
     4861 tcatgaaaac ctcccatttc attttagtca ataaacaatt gttggatctt ctgatcaaag
     4921 ctctccggtt gccattgttc attcaattgt tcacgaaaaa caaggctaca tgttttttta
     4981 tgaaaattat ttaaataccg gtcgtaataa ccaccgccaa aaccgattcg ataaccggct
     5041 ctattgtaga taatacctgg tacaatgagg agatcgatca tctccggcaa atagagcttc
     5101 tctgattgtg gctcaagaac accaaaactc gaacgtacaa gaacggactg ttcatccatt
     5161 tcataaaacg ccatctgacg tttcggcaaa gttttaggta caactatttt cttttgttct
     5221 ttttctgctc gttggataac cggctgtgta tccagttcaa attcattcga taaagtcgtc
     5281 cctatcactt ttgctgactt ccagtactcg ctggcgaata gctgtttata gattcgctgt
     5341 tctttttcct ttttcttgcc acttttggcc agatttttca gcttttcgat tcctttccgg
     5401 cgtaatctta ctttcaattg gtccaaaaac tcacccttcc ttctctcata aaatggtata
     5461 ggaaaaaaag aaaaatagca atcgaacttt caaataaatg cataaataga ataaaaagaa
     5521 gacctagaaa tgattggcct tcctttttta atgaatttca actgttcagc gcgtgttttt
     5581 gtctgataaa aagtgcatac agaaaatatg gcgcaccaaa aagggcaatc aaaattcctg
     5641 taggtacttc cccacttagt agaatcactc tagcaaccat atctgcacta agcagaaaaa
     5701 tggaaccgaa caaaccgctc agaacaaagg tccagttatt ttctttacgt acgactagct
     5761 tcgcagcatg aggagctagt agtcctacaa aagataaact acctgtcacc gctacactac
     5821 cgcttgccaa agcaacagct aacaacaaaa ggattttttg acttttagcc acgttaaggc
     5881 ctaatccttg cgcgcgttct tgaccaaatg atagcaatac aatcctctgt gcataaagtc
     5941 ctcctagcgg aattaagata accgcccatg gaagcaatgc ccaaacataa ggccagctcg
     6001 caccccagat tgatcccgct aaccatgcat tcacaaatcc ataattttcc ttagataatt
     6061 ggatcgtgca tagcaatgtt aatgcagaaa ggccggcatt aacggcaata ccggacaata
     6121 agattcgatt catacccaaa aagccatttc gatcataggc cgcaagatag acacagcaag
     6181 cagctaaaac accacctaga aaggctacaa ctagcaatag aaacggcagc gaatagtgcg
     6241 aaaagaaacc taaataaagc atcactaaaa atcctgctcc ggcattaatg cccaatatac
     6301 tagaatctgc caattcattt cttgttactc cttgcaagat ataaccagaa acgccaagac
     6361 aagctcctgc taataaactg atcagtaatc gaggaactcg aaattctcta acaatcagcg
     6421 ccaaagaaga tgattctttc cctatgaacg tggcaaaaag ctgatccagg taaataaaag
     6481 gcgtaccaat cattaaacta cctatagaca ggatgacaag tacaaaaagc aagaaataga
     6541 acgtccaata tatttttttc accgttgttc cctccttact tgtatcagta aaaacggaac
     6601 gccaatcaat gcaatgacta atccaaaagg tgtttcaaaa gggggattga tcatcctcgc
     6661 tgtaaaatcc gccaacataa ccaatccacc gccaactaaa aaagagacag gtagaacata
     6721 acgatagtcc gttccaacca ttccgcgaac aactggcgga accaacagcc caataaatga
     6781 aacaggtccg actaacgaaa cggcaatggc tgctaacact gcaactagac agaaaacaat
     6841 caaccgcgtt cgtttaggat gacttccaag agctaatgca tgtgtttcac tcaaatgaat
     6901 caaagatatt gcttttccta atgaaaaaga aaccaaaaca gcaagtatat ataaaggaaa
     6961 caatactgct aattgtttcc atgtgatatt ggcagctccg cctacaaacc aaaaagcaag
     7021 atcctgctgc agattaaaga attgggtaag tgcttggctg atagcagtaa aaaaagaact
     7081 aatagctacg ccagctaaaa tcagctgtaa aggattcatg cccattttcg aaaactgcaa
     7141 aaaaccaaat acgagcaaag ttacagcaaa agaacctgcc aatgacatta gaaacacaga
     7201 aaaatcagaa gctttaggcc aaaaagcaaa actaagggcc attgccaacg aagcacccgc
     7261 gttgattcct agtaagcctg aatctgctaa aggatttctt gtcactcctt gcatcaatgc
     7321 accgctcaat gcaaaacaac tccctacaag aaatgctccc gccacacgag gcaaacgaat
     7381 atttcgtatg atttggtgac tttgttttga tctatcaaaa tcaaacaacg cctgccaaaa
     7441 atctgctttt cccacagtcg ctgcgccatt tgaaagagca acaaaaaaaa taatcaaaag
     7501 aacaaaacag accaaaagca ggagtctatt tttcttcata gtcctcataa ttgatcctcc
     7561 tgctcttttg gcaacaaatc ataggttaga aaaactggtt tatttgttct tggaagcgtg
     7621 acaatcgttg catcaatctc aaatattttt ttcatacact cttttgtgat cacttcttca
     7681 ggtgtacctt ctgtgatcaa cttaccgttt ttcattgcta ataaataatc tgaaaaacgt
     7741 gaagcatgat taatgtcatg aatcgacatc aagatcgttg tttgtttcat ttgattgatt
     7801 tcagtcaata actgtaggat ctctaattgg tgtgccggat ctaaaaatgt tgtcggttca
     7861 tccaagatca agatatctgt ttcctgagca agcgccatag cgatccagac acgctgggtt
     7921 tgtccaccag acagcgttgc gatatcccgc atacgaaatc cagctagacc tgtagcatcc
     7981 aacgcccact caattgcttc atgatcttgc gtgtccatgc cactgaacag tttttgataa
     8041 gggtatcgtc cgtaagatac tacttcttct actgtcatac ctgtagggtg ttcactagtt
     8101 tgtgctaata atgcaatttt ttttgcaagt tcttttgaac tgtatgtaga gatatcagag
     8161 ttattgacaa tgatctttcc tgatttcaca ggtagtatac gggtaaaact tttcaataaa
     8221 gtagattttc cacttccatt tggcccaatc aaactagtga ttttcccttc aggaatcgta
     8281 acagacatgt tttcaatgat ttttttgttt tcgtagccgg ctgcaatgtc agctgctctt
     8341 aatctcgtca tttcacccat cctttcgctg aaaatcattc tcaattatga cgctgtttca
     8401 acatttcgtc aagcgctact ttcatatcta tagccaaatg aagcggttga cagcgttcga
     8461 aatatcagga ataataacca atgagaaaga ttctcaatta tgttaggagg aaagaaatga
     8521 aattaaaaac aaaagtattc acattatcta gcatattgct gctggctgga gtactgagcg
     8581 cttgctcccc ctcttcagat aaagaaacag ctacaactgc caattcgacg acttcatcca
     8641 atagtactca tacagtcaca gatacacttg gacataaagt agagattcca aacgcgccca
     8701 aacggatcat cggcagttac ttagaagact atctgattgc actaggggaa aaacctatcg
     8761 cccagtggac tgttggcagt ggttccattc aagattatct gcaagatgat ttaaaggatg
     8821 tcccaacgat ttcttatgac cttccttatg agaaagtgtt aagctttgag cctgatttgc
     8881 tgttgatttc ttcttccgct actgtcgaag gcgggaaata cgaacaatat agcaaaatcg
     8941 cgccaactta tgttgtaaaa aacggaacag atgtcacatg ggaagaacaa ctaaaagatg
     9001 tcggcaaagc tttggcaaaa gaaaaagagg cagaaaagat catttccgat tatcaaaaat
     9061 ccatcaagaa aactcgagaa gaactagcag ataaaatcga gaacaagaca gctgcagtgt
     9121 tgtgggtgac caacaactca gcctttatgg tcagcaaaaa tcggtcaagt ggaagaatcg
     9181 tatacgacga tctggaattt ggtgttcctg atctagtgga agaggtctcc aaaaaagcca
     9241 cttctgactg gtctgctgtt tcctctgaaa agctagctga attagatgct gactacatca
     9301 tccttgtgaa cagtgacaaa gatgctgcca tgttcaatga accaagctgg aagaatctta
     9361 aagcagtgac agacaatcat gtactgcaat tcggacctga atctagttgg ctatataacg
     9421 gacctattgc cagtcaggaa atggtcaatg atatcaaaga aaaactaaat tgattttaac
     9481 taaaaaaagc atcttcacga ttagtcgtga tatgcttttt ttcttattaa caaactagac
     9541 gcatagattt tattcttctt ttgaagcaac cctatttttt actctttccc atttttgaaa
     9601 acgatgggca tatagatttt cttgatccgt tatcccctca tactcctcta taagggcgaa
     9661 gtctgaaaaa tcaatattcc caatatacgt atcgccttca aacgtgctat cgatcaatgt
     9721 tcgccagatc cgaccagtgt aaggaagaag ttcttgaaaa atccgactac cacccgaaac
     9781 atagatatct tctgagatcg tttgactata cgacaaaagt tcatcgatcg aatgcattac
     9841 ttccgcattt tctttcgtgc gaaaatttgg ctgcgttgtc aacacgatga tatggcgata
     9901 aggaagtgaa agatccccca ttccttcata ggtctttctt cccatcacta aaattcgatc
     9961 cattgtatgt tcgcgaaaga aacgcatatc atttggcaat cgccatggta acaaaccatt
    10021 tttgccgatc agaccatttt tatcttgtgc ccacatggaa ataaacatat tatccgctcc
    10081 ttatgatttt agatgttttg agataaaaaa agtctcaatt ctttgaccag caaggaattg
    10141 agaactttaa aaacattatt ttgtttcacg gtgcaaagta actttgcgtt cgcgtgggca
    10201 atatttttgt ttttccaaac gatcaggatt gttacgtttg tttttgcttg ttagatagtt
    10261 acgttcttta caagaagtac actctaaagt gatgtttacg cgcatgtttt tccctcccat
    10321 ttttacaaga atcctctaag atgactctca gacttgat
//
LOCUS       DACDIE010000081         9949 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.081, whole genome shotgun sequence.
ACCESSION   DACDIE010000081 DACDIE010000000
VERSION     DACDIE010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9949
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.081"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            335..2170
                     /gene="tdc"
                     /locus_tag="IV250_002434"
     CDS             335..2170
                     /gene="tdc"
                     /locus_tag="IV250_002434"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP8769679.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            2475..3953
                     /locus_tag="IV250_002435"
     CDS             2475..3953
                     /locus_tag="IV250_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8769680.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            3919..5250
                     /locus_tag="IV250_002436"
     CDS             3919..5250
                     /locus_tag="IV250_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8769681.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            5364..7916
                     /locus_tag="IV250_002437"
     CDS             5364..7916
                     /locus_tag="IV250_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAP8769682.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(7923..9362)
                     /locus_tag="IV250_002438"
     CDS             complement(7923..9362)
                     /locus_tag="IV250_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8769683.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            9708..>9949
                     /locus_tag="IV250_002439"
     CDS             9708..>9949
                     /locus_tag="IV250_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769684.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGK"
BASE COUNT         3168 a         1634 c         2103 g         3044 t
ORIGIN      
        1 gaaaacagat aattctatta aaaaataaat tgcgaatttt taacaatata aaaaaataaa
       61 aatatgttta gtgaatgaag gacagtcatt ttatttgttt gttcgctttt tttaggttat
      121 ctattttaga agactgattt attgaaaaat tgttcgcttt tttgcgttga attatcagaa
      181 aaatccgact ttatttattt ttgtttgtaa aaatctacgt ggatttgatg ttataaaaaa
      241 aaaattctca tttttggcgc tatagtgaag aggtgttaca gataaacaca tgtatctgca
      301 agacattgac caacaaaata tggagggaag aaaaatgaaa gatatggata tcaaggccgt
      361 ctttataggc gacaaagctg aaaatggacc agtttataaa atgttgttga acaagatggt
      421 agatgaacat cttggatgga gagaaaatta tattccttca gatatgcctg caattagcga
      481 aggagataag ctcactccag attatctagc cacccgcgac catatgatag aggtcttgga
      541 tgaagtcagt cagcgattac gcgcaggatc gatcccatgg cattcagcag gccgttactg
      601 gggacaaatg aatgctgaaa cattgatgcc tgctttatta gcttacaact atgcgatgct
      661 gtggaaccca aataatgttg cattggaatc ttctatggca acttcgcaaa tggaagcaga
      721 agtaggacag gattttgctt cattattcaa tatgacagac ggatggggac acattgcagc
      781 agatggttct attgctaatc ttgaaggatt atggtacgcc cgttgcatca aatcgattcc
      841 tttagctgtc aaggaagtgc tgcctgaaaa agtgaaaaaa atgtctgagt gggaactttt
      901 gaatttatct gttgaagaaa ttttggaaat gaccgaaagc tttacggacg aagaaatgga
      961 tgaggtcaaa gcagcttctt cccgtagcgg aaaaaatatc caaagattag ggaaatggct
     1021 ggtccctcaa acaaaacact attcttggat gaaggcgtta gatatttgcg gagtcggctt
     1081 agatcagatg gttgctatcc cagttcaaga ggattatcga atggatatta atgctttgga
     1141 aaaaacaatt cgtgaattag ctggacaaaa aatcccaatt ctaggtgtgg ttgctgtcgt
     1201 aggaacgaca gaagaaggtc aagtagacag tgtagataaa atcgtccaat taagagaaag
     1261 gttaaaagat gaagggatct atttctattt acatgttgat gccgcatatg gcggctatgc
     1321 gcgttcactg tttctaaacg aagcaggaga atttgtgccg tatgcttcct tagctgaatt
     1381 ctttgaagaa catcatgttt tccaccactg cgtaacgatt gacaaagagg tatatgaagg
     1441 gttcagagct atttcagaag cggattctgt cacgatagat cctcataaga tgggctatgt
     1501 cccttatgct gctggaggaa tcgtaatcaa acataaaaat atgagaaata tcatttctta
     1561 tttcgcgcca tatgtgtttg aaaaatcagt aaaggctcca gatatgttag gagcttatat
     1621 tttggaagga tcaaaagctg gagcaaccgc cgccgcagtc tggacagcac atcgtgtatt
     1681 gccattgaat gtgaccggat atggtcaatt gattggggct tcaatcgagg cagctcaaag
     1741 attcagagaa tttctcgatc atctgacttt tactgttaaa ggaaaaacaa tcgaagtcta
     1801 tccattgaat catcctgatt ttaatatggt caattgggta ttcaaagaac aaggctgtac
     1861 agatttgaac gctatcaatg aattaaatga aaaaatgttc gatcggtctt cttatatgga
     1921 tggcgatgtt tatggtgaac ggtttattac ttctcatact acatttacac aagaagatta
     1981 cggtgattct ccgattcgtt ttgttgaaag aatgggtcta acgaaagaag aatggaaaaa
     2041 agaacagaaa atcactctgc tacgtgcggc tatcatgact ccttatttga atgatgatcg
     2101 aattttcaat ttttatacaa aaaaaatcgc caaagcaatg gaaaagaaac taaacgaaat
     2161 catccaatag aactgatttt tgtgtgaaag aataacaaga gacatatgga gaacagacaa
     2221 ttatttgctt catcaaaaca attgtatttc cccctgtttt tcatatattc ctcgccaaga
     2281 tattcttttt tataaaacgt tctataagtt ggatcgttcg tttcatgtat caaacccagt
     2341 tcactaagat cgttcaatgg atgtttcggt gatactgggt tttgtactga atcgatgaaa
     2401 ccttgtcatt tctgttcttg aacaaaaata aaagaaaaca aaaacatatg tcgctattga
     2461 aagaaggaat aaagatgaaa gatactggtt catcaaagca aatcacgacc atgaccttta
     2521 tcggaatgac ctgcgccctt gtagcaagtg tacgaaatat tccagatgta gcagcaacga
     2581 gctggacgat gattttttac atgctggttg cagtattatt gtacgctttt ccaatctcat
     2641 tgatctctgg tgaatttgct ggaatgtttc cccaaaaagg tggtccagaa ctttgggtat
     2701 cgaatgctct aggaaaaaaa tgggggttcg ttgtctcatg gctgttatgg gtccaaatgt
     2761 ttcctgggat ggtgatggtc gcttcagcac tcgctccgtt gtttggtaat attatcgata
     2821 atgtgccgct tggtttgaac agcaaattta cattagttgt cattttagtc gtttattgga
     2881 tcatcacttt tttgaatttg aaatttgata tggcaaaaat cggcggaaaa gtcggtgttt
     2941 ggttaggatt gtacgtgcct ttgacgatga tgctcttatt agggtttgct gcgtggatca
     3001 aagttgggat cgtaccaacc ggaacattag gcagtttttc atgggacaaa ttaataccag
     3061 atactacgac tgcatcctca tttgtttatt ttgcgccgat catgtttatt tttactggta
     3121 tcgaaatgtc ctctgtgtat atcactcgat tggaaaatcc agttaaaacg tatattcgcg
     3181 gagtatttgc cgcgttaatt tttatatttt tcgtcaatat tttgaatgct ttagttgtag
     3241 caaatgtcgt accaaaagga caaatggaat tgaataatat cgcgcaatct atttcgattt
     3301 attgccaaat tcttggtttg ccacatctta ttgtgaatct gtttagttta cttgtcttca
     3361 ttggtgtagc agtccaatta tctgcttggg cctcaggacc agcaaagaca gtgacagaaa
     3421 gtgctagaaa aggtgcgtat ccaccaaaat tcaatttttg gaagacgaat caatttgacg
     3481 tatccaaatc cgttattttg actcagtctg tcattatctc aatctttgca cttttttatt
     3541 tgctgattcc tggcgtcaat caagcatttt tgatgctcgt aaattcaact actgttatct
     3601 actgtatcgt ctatgtcatc atggggatcg cagttttgag attgcgttat acacatgcaa
     3661 agttgaatcg accatttaga attggaaaaa aagagaaaaa gagcaatctt ggagtttgga
     3721 ctgtcgttat tgttttattt gcggcaatcg gattttctgt cggactgacg atgaaagctg
     3781 gaacatggat caatctaatt gcagtcacag ccatcagtgt cattttgttt gtagtaccac
     3841 tctgtattga aaaaataaag aaaccttctt gggaacagga agtcgccatg ctgcttgaaa
     3901 aagaaaaagg tgaacttgat ggaaaaaccg gcaatgaaac ggactctaac taatcgaaat
     3961 atccagttaa tcgcattagg tggcgcaatt ggaacaggat tatttttagg ttcagcaaag
     4021 gcaattgcat cagcagggcc agctgttatt ctcgtttatt tgatatgcgg tttggctatt
     4081 tatggtttga tgcgggccat gggtgattta tttttagcaa atagtacatt taactctatc
     4141 agtgatttcg ttgactatta tttaggcaaa aagtgggcat tttttgtcaa ttggacttat
     4201 tggctttgct ggcttggagc tggacttgcg gaattaacag caataggatt gtatatccat
     4261 ttctggtttg tacatgtacc agctttgata agcggaggta gcgcactaat gatcttaatg
     4321 gcagtcaacc ttgttgcagt caagtggttt ggtgaattgg aatctttttt ctcaatcatc
     4381 aagatcttgg ggattttcct ttttatctta ttgggtatag gattgagtgt ctcagatttt
     4441 catacccagt ctatcggaat caaagaaaat tttggacaga tcgatgaatt actaccgttt
     4501 ggctttgctg gaatggtagt ctcatttcct atggttttat tcagttttgc tggaattgaa
     4561 atgatcggct taaccgttgg cgagacaagt gatccgcaaa agaatttgaa aaaagcaatc
     4621 aactgtctgc cttggcgtat tctctttttc tatataggaa cgattttgtc gattttattt
     4681 gttgttccat ggcattgttt agatttaaaa gaaagtcctt tagtgactat gctgcaagtg
     4741 atccattgcc aaggcggagc agcaattgtc aatctaatga ttttggttgc ttctttgtcc
     4801 tcagcaaata gtgcagtcta cagtactagc aggattttat atcaaacagc aaaagaaaaa
     4861 agtttgcccc attggttttg tgcgctttct aagaaaggcg tacctttaca cggtatttta
     4921 attacaggaa tgttattttt tgccggtctt tgtctacatt tttttgtagc tgatagtcga
     4981 ttgttttttc agcttttggc aggcatgaca acgagttgtt ttctatttgt gtggtcttcg
     5041 atcttgtgtg cacatgttca atttattaaa gaaaagaaac ggaacactac acaaagatat
     5101 aaagatcttg ctttgttggt gttttttact tcaattgctt tcagcttgtt attcgaaagc
     5161 acgacacgct ttattccttt tttattggtt atctggtttg tattcttaaa ttttgtatat
     5221 aaaagaataa gcaatagaaa tatcagttag gcccagatac attttgcaaa acgaattatt
     5281 tcagaccccc ctgaaataat cgatacgcct gaaaaaactt ccctgcgtat aataacagta
     5341 tcaattaaac aggaggggat aggatgaact ggtacagatt gacggtagaa gatgtattga
     5401 aaaactatca aacatcaaca gaaggcttgt cgcaagaaga acgacagaaa cgcctgactg
     5461 atcaaggata taatgaaata aaagcaaggc agcagacaaa acgatggaaa aaaatagcaa
     5521 agcattttac tgatctgctg atgatcgtgt tgatcattgc ctcttttttg aaatttgctt
     5581 ctagtgaata tattgaagga agtattattt tattcgtagt gatcgtcaat ggactcgtta
     5641 gttactggca agaaagaaaa gcggaagaat cattgaatgg tttgaagcag ctgatgggac
     5701 aagaagcgat agtactatca aacgggatgc aagaaaaaat ccctgcgaaa gaactggtaa
     5761 aaggagatgt cattgtctta cgtgccggag atgtcgttcc agcagatatc cgattaatcg
     5821 aagcacatga tttattagtg gaagaatcga ttttgacggg agaatcagaa gcatctgaaa
     5881 aaagtcatgc cgttttgaca gaagaagaat cgatcggcga ccagaaaaat atgggttttt
     5941 caggaacgct tgtacaatct ggttctgcag tcggtgtcgt tgtagaaaca ggaaacacga
     6001 cagaaatcgg taaaatcaat caagcattgc aatcggtcga acaacaaacg acgcctctga
     6061 ttaaaaaaat caaccgatta aacaaacaaa tttttcaagg cattctttgt ttgagcctat
     6121 tcttagttat ttttacgaca tttcgatatg gtatggattg gcatatcctt ctttcggcta
     6181 cgattgcttt ggtggtgtca atggtacccg aaggattgcc agctgttttg accatgattc
     6241 tatccgttgg agtacatgaa atggcaaaag aaaaggcgat catcaaagga cttccttcgg
     6301 tagaaacatt aggttctatg acagtgatct gttcagataa gacaggaaca ttgacaaaaa
     6361 atgaaatgac agttgtggat gtagctgcaa aagaagaagc ctgcgtgttg tccattatga
     6421 aaaactgtca ggaattaaaa acaaaagaag aacagaaaac agaaaattta agtggcaatc
     6481 cgacagaagt tgcgctgatt cgacacacag aaacagccaa tctacctttg aaaaaaatag
     6541 aagcaaagat tccgttcagt tctgaataca aatatatggc aacgatgcat gcggaggaaa
     6601 aaggagcggt gatttacgtc aaaggtgcac cagaagtatt atttgctaaa tcaactcttt
     6661 cttctgcaga acaagaagca tggagtcaga cagctgcaga atttgcgaga aaaggtcagc
     6721 gtgttttagg atttgcttat aaaaaagtag attcgaaaca ggaactcacg catgagactt
     6781 taacccaatt aacttttgca gggatcgccg gattgatcga tccgccaaaa gaaagtgcag
     6841 tcaaagctgt aaaggaatgc caacaagcag ggatctcagt gaaaatgatt acaggggatc
     6901 acaaagacac agcaaaagcg attgcggaac aaattggctt aaaacatact gccaaagttt
     6961 tagaagggat cgatttggat cttatgtctg atgaagagtt gattcagcaa gtgccaatag
     7021 tcgatgtctt tgctcgaacg acaccagaac ataaactgcg aattgttaaa gctttgcaga
     7081 aaaatggaga aatcgtcgga atgaccggag acggcgtaaa tgatgcacct gctttgaaaa
     7141 gatccgatgt aggaattgct atgggaatca aaggtagtga agtaagcaaa caggcagctg
     7201 atatggtttt aggagatgat aatttccata cgattgcaaa agccgtaaaa gagggccggc
     7261 ggattttgga taatttgcaa aaaaccatca atttcttctt gccaactgca ttagctcaag
     7321 gattgatctt gatttgggca cttatgctga atcgcccgct acctttgtcc ccagtacaga
     7381 ttttatgggt caatatggta acgacaatca ctttatctta tgcactaggg ttcgagtcgc
     7441 cagatccaga aatcatgaag cgcccaccaa gagatccaaa gcaaggaatc ctatctggtt
     7501 atcatttgtt tcggatcatc tatgtctccc tactgatcat ggttcctgcc tatttattgg
     7561 ctatgcagtt tgaagggcaa agcttacagc aaaccattct tctgcaaaat atcgtcttag
     7621 ctcaagctgt ttacatgatc aattgcagag agttgtccaa gccgtcattg aaccaaggtc
     7681 ttttgcaaaa caaagcgctt tttctttcat taggtatcct tgctttattg caagggatgg
     7741 ttatttttct accagtcggc cagcagctga tcggtacagt aagtttaacg atgacacaac
     7801 agttattgat tggagcaaat gtgattcttc tattcttagt tgtagaaata gaaaaaggga
     7861 tcatgaacaa actgtttcgc aaaaaagaaa aaacagctag agaatacagt cgctagccgt
     7921 tactataaaa aagttttgaa agtgagttca gtcaaacgat tgagctcatt tttcgttgga
     7981 atagaagaaa tgtatacaat agggatatct agagaatcta gagggaaatt ggaaatcaag
     8041 atatctgttt tttcaaaaga aatagtggaa agatcggtta gttcgatagg taagaagcag
     8101 acacgttttc ctaagtaatc ttttaattcc tgctgcaaga acgccttcca tgaaggttct
     8161 ccttggaaaa taaaatagac attgaacaaa ttcggtttga tagaaaaaat cgtttgttgg
     8221 atcagtagag acaagagttg gagaaagtaa tttggttcat tgatttctat ttcttttttt
     8281 agatcgttca gtaaattctc tttctgttga tacaagtgtg catattgctg atcatatgtt
     8341 tctaaaaaac gatggtattg acggtccaat gcttcaatca atatcggcga ttcatgatat
     8401 tttaatatga ggagagccaa gttatcagtc agagatgttt tttttagttg atcaagagac
     8461 agctctgaag ctagttcctc tacaaacgtt tcgaggacct ctaaaaatgg agaatgatgc
     8521 aggcagcgag cggtttcttg tccatagtta ttgttgtaat tccaactttc cagaaagcag
     8581 aaagaagcaa aagaaagctc agtgtcttta agataaatac cttccgttaa atacaatttt
     8641 ttcaatgcag agtcatacag ggaggagagt gtgctgttgg tatggataga agaatctaat
     8701 cggcaattcg cttttatacg gatcgtattg ataaagaacc aacatgttcc gaaaatctct
     8761 tctgtttcaa ccgctagttc agtttgactg aggtttctta aaaattgaaa ataatgggat
     8821 tcatggatcg aataatcctc gaaaaaatag ttgttatgag tgaaaggaat cagcaatcga
     8881 tgataaaaag tacgaatcgc acgctctttt ccaagaatac aagctgtata tttcgtcaaa
     8941 tcgatctgca agtcgtaact tgctaaacgc tgattgattt tagtcacatg tcttttcaat
     9001 gtcccataag aaagcccatg gtcggctaga aaatcaggga cagcaacttt ttttgtaaaa
     9061 agcagatttt tcagcagttc gacactgact gattgtttga aaaaaatctc ccaaaggtca
     9121 tttgttaata aattttgctg gttttgcaaa ctgattcctg cttgatcgct gatgatgtcc
     9181 cagccttcgg gcagctgaga gcgaatagat tgaaggtcag aaaaaatcgt tcgttcggtt
     9241 gtccctatga tttcagctaa ttcatttacg gttatctttg tatgattcag caattgttct
     9301 actaaagaaa catgacgtcg aatatctttt tcggtaatga tttttttcat tagtgaatac
     9361 atcaatcaga ctcccttcta ttctaagaat acggagaaaa taaacggtgg tcaagtttca
     9421 actgattttt caatcattgt ttatttttct ggagcattta ctgatgataa gaatttcatc
     9481 atgaacttga aaaatgatac cactaaaatc aaaaaatagg tgggcaaaca ataataaaca
     9541 ttttaatagt agatggtagc aagaccaaca caggcggatt gaacatttaa gagttgataa
     9601 aaaagaaaaa tatcgaagta tttgattagc aactacttgt attcattttt ctattcttac
     9661 gtggtatctt tgaataaagc gaatcaataa gaaagagatg gaagcagatg ctaacgaaaa
     9721 aagaactaat cgatcacatc aatgaaacat acaatttgtc tccagaatat atttttaaaa
     9781 aatttcctga ttattgcgta ttcagacatc aaaaaagcaa aaaatggttt ggggtcctga
     9841 tgtctgttcc agaagaaaaa atctatgatg atgcaagtga ccataggatc atcaatattc
     9901 ttgatttgaa ggtcgatcct gaacttggcg gaattttgcg aggaaaaga
//
LOCUS       DACDIE010000082         9941 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.082, whole genome shotgun sequence.
ACCESSION   DACDIE010000082 DACDIE010000000
VERSION     DACDIE010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9941
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.082"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(402..626)
                     /locus_tag="IV250_002440"
     CDS             complement(402..626)
                     /locus_tag="IV250_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769685.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            826..3594
                     /locus_tag="IV250_002441"
     CDS             826..3594
                     /locus_tag="IV250_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8769686.1"
                     /translation="MLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSH
                     YLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQPKDL
                     FSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGELKNI
                     VKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELEQQV
                     SQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPEWRL
                     LYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNSDPL
                     FSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQRII
                     RNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSEDTE
                     FLSIEEEF"
     gene            3675..4094
                     /locus_tag="IV250_002442"
     CDS             3675..4094
                     /locus_tag="IV250_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAP8769687.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            4106..4576
                     /locus_tag="IV250_002443"
     CDS             4106..4576
                     /locus_tag="IV250_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8769688.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            4593..5372
                     /locus_tag="IV250_002444"
     CDS             4593..5372
                     /locus_tag="IV250_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8769689.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            5359..6180
                     /locus_tag="IV250_002445"
     CDS             5359..6180
                     /locus_tag="IV250_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8769690.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            6197..7246
                     /locus_tag="IV250_002446"
     CDS             6197..7246
                     /locus_tag="IV250_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8769691.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            7259..8263
                     /locus_tag="IV250_002447"
     CDS             7259..8263
                     /locus_tag="IV250_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8769692.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            8896..9915
                     /locus_tag="IV250_002448"
     CDS             8896..9915
                     /locus_tag="IV250_002448"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP8769693.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         3335 a         1646 c         2079 g         2881 t
ORIGIN      
        1 gtatgaaaat gagaaaaata tttattcgtt tattgtgggt actatcttag aggaataaat
       61 aatagtggag aaaactatgg tttggcgcag attctatcag gtttgatgga acgaagacaa
      121 cctcccgaca tcaagtcaaa agctacttga gattttgatc tgaaaataaa aaaagaaaac
      181 aaggtaggaa catcaatgtt tctaccttgt tttcgtaatc aatgggaaaa aactaagagt
      241 ggtgtacgta atcaataaaa aggatggtga caacagtacc tctttactct ctgaaagcat
      301 cataactcgt ttttcctcat tatgccaata aattgttgtt ataccacata taaggatttg
      361 taatagctaa aaacagacag gaaagttgaa atctgtgaat cttattttgt atggaaggat
      421 ttatagaacg caaaaatcaa gataaagatg aataaaaatc ctgtttcggc tatatattgc
      481 gtatctacgc caataaaacg agtttgttct gtcatagcca tataaagcaa aggcaaaaat
      541 acaactacag cgagataaga tccgatgatt tctcgaaaag ttttccagga agtatgtttc
      601 ctgtttttat atttatccga tttcattgaa tagtcatagc ctccttattc tttcatatgt
      661 tgaaattcca gtataaaaac tggaaggaac tgtacgtttt ttagaataaa aattataagg
      721 gataagtagg ctgctgtcta taataagata tatttttcaa ttctttatta aagattttat
      781 tgacaaaatg aatccgtttt ctatgatgga ggaggggtga ataagttgtt gaagaaggaa
      841 attttatgtt acttagagaa ccagacagcc tttttcgatt tagaacatat gaacgaggtc
      901 tttacagcac aaaggttagc tgatcatttt ggggtaaaaa gaaacacagt cagtcattat
      961 ttgaaccagt taaatgatga gcaactacta gtcaaaatca attctcgacc agtgatttat
     1021 ttccacaaag aagcttttga aaaacaattt tttaagttac gcacgaattt ttatttttct
     1081 atcgatgatc tcaaagagga acagcctttt ttcgcgcaac caaaagactt attctctttt
     1141 atgatcggat acgacgccag tttgaaagaa agtatcgaac aaatcaaaac agctctttac
     1201 tatccagatg gcggattacc acttttgatt acaggcgaaa gtggcacagg taaaagttat
     1261 ttagttaatt tggtctatca gtattgtctg cttcatgatc tgctagaaga ttctgcacca
     1321 ttcatcactt ttaattgtgc acaatatgca gataatcctg aacttttgac tagcaatttg
     1381 tttggacatg ttaagggagc gtacactggt gcagaagaaa ataaaaaagg ggcttttgaa
     1441 gcagcagatg gtgggatctt gtttttagac gaggtacatc gcttaacacc agaaggtcag
     1501 gaaaaactgt tcacctatct tgatcaaggt gtgatttatc gtatgggaga acctaatttg
     1561 tcacgccgga tcaaaacacg tctctttttt gctacgactg aagagattac tagtcatttc
     1621 ttaacaactt tcattcgaag aatacctatc cgaatcgaac tacctgcact taatcagcgt
     1681 agtcgtaatg aacggctaga actgatttac tctttttttc tcgaagaaca aagaaaaatg
     1741 gggagccctt tgtcagtcag tggaccagct ttgtcacttt taacaagtca gaaattatcc
     1801 ggaaacatag gagagctgaa aaatatcgtc aaagtttcag cagcaaaagc atatgctgaa
     1861 cagcgtgaac agcctgaaat ccatgtcacg atccatcatt tgcaaaaaga actacttgcg
     1921 cagcctatta ctaaaaatac agcacagcaa ggaatctata ttacgcaaga ccagacctta
     1981 gagcaattaa tggagaagca acagcccgaa cagcagcgaa tcgttaaaag ttttgagcaa
     2041 atcctaattg atttcaagaa aaataactgt ctgctatatt cttgcgaagg aaaattaaag
     2101 caagaagtga ttcaattatt tgattttttg ctgtttgaga ccagtagaca agaaaaacac
     2161 gagctgctgg tttattggac acaatctatc cgagagacct ttcgacaaat ggaaactgcc
     2221 tatcaaatca aattcgatgg aaacagtgtc tatgccttga gttactatct ctatcagcga
     2281 agcactatca aatggttacc ggaagaacat gatattatcc aactaatcaa agaattagaa
     2341 caacaagttt ctcagtctta tccatctagt tatcactatg tccaacgaat tctggaatta
     2401 agtaagatca aattagatat tgaagacaca agtatggatc ggattttact gacgatctat
     2461 ctaaaaaaag cgaaatggtc aaaagaaaaa cgactgccaa aagcgatgat tgctgctcac
     2521 ggttatgcaa ctgctagcag tatggcaaat gtagtaaatc gtttattaca agaggatcta
     2581 tttgaatcat ttgatatgcc actagatgtc acaccacagc agattgcttc agaaatcctt
     2641 gattattgcg aaaacagcga tgtttcaaat ggattgatca tattagtaga catgggatct
     2701 ctgaaagaaa tccatcagtt ttttaaaaaa cagttatccg taccgctttt gattttgaat
     2761 aatgtgacga cacctttagc aatcacagta ggggaatgcc tgcaaaaaaa tgtatcatta
     2821 gaagagatag cagaagagac tgttcgacag atccagccag aatggcgact gctctatcca
     2881 gaggaaaaca aacccaaagc actcattact acttgcttca ctggaatcgg tacggcggtc
     2941 cacttaagtg aactgctaga aaaaagctta cctacggctt gtcagttgaa aatcatccca
     3001 tatgaatatc agcaattaaa agataagaaa aacagtgacc cattattttc catatacgaa
     3061 gtcgtaggaa tgattggcac cacagatcct gatatcacca atatcgacta tctttcatta
     3121 gaagacctca tctcggggga aaagatgtct gtactagctg aatggttgga cactactatg
     3181 aatgcttcag agaaggaaat attcaatcag cgcatcattc gtaatttttc attggaaaaa
     3241 gtactggatt ccgtgactat tctagataca gagaaggtga tgggagaaat cgatcatttt
     3301 atgagagaat tagaggtgca gttgaatcga agcatcagca atcccaaaaa attagcgttg
     3361 tatgtccatg tgagctgtct cattgaacga ttgatccgac aaatgccaat cgaaacctat
     3421 caaggattag agaaatggaa gcagtgtcaa aaagaagggt tatctgctat taaatctgcg
     3481 tttagtgtca ttgaagagca ttatagtgtc atgatcccca attctgagat tgcctatatc
     3541 tatgatattt tgagtgaaga tacagagttt ctctcgatag aagaagaatt ttgaacataa
     3601 gcaagttgtt gccactaatt ttggcatgca acttgctttt ttagtatgtg taagtgaaaa
     3661 gaggagatga aaaagtggat agagaaatta tcttagcttc ccatggaaaa tttgcttcag
     3721 ggatattaga ttcattagag ctgatctatg ggaaaaatca ttctattact gtattcgatt
     3781 gctatactga tgagaatttt gatttagcgg aaacagtaag gcagcttttt atgaggtaca
     3841 aagataaaga aatcattgtt ctgacggatc tttttggcgg aagtgtcaac aacgaattct
     3901 tacaatatat caatcgggat catgtctatc tcgttgccgg attgaatttg ccactattga
     3961 ttgagctagt atctcggttg gacgctgaag tagagacaac tgttttgatc cagcaggtgc
     4021 taactgattc aaaaaaaatg atccagtttt gtaacgagcg gctcaatcag aaaatagaag
     4081 aagacgaatt ttaggaggaa gaaaaatgat tgtattgaca agagtcgacc atcggttatt
     4141 acacggacaa gtagcttttt catggacaca aacaataggc gcagactgta ttttgattgc
     4201 taatgatgat gtacctacga atgagatacg aaagacaacg atcaaattag ccaagccaca
     4261 aggagtcaaa ctagtgatca aatcaattga tgactcgatt gccgcgttga aaagcggggt
     4321 gacagataaa tataagttat tcatcgtagt ggaatcgatc gaggatgcat ataagctggc
     4381 agaagcgtat ccagaaatca aaaccatcaa cttaggaggg atcaaggcaa aacctggtac
     4441 tcgcagcatc ggaaaagcgg taaatatttt accagaagag gaggaacgct tgaaacgatt
     4501 aattgacaaa caaatagaag tagaaatccg tcaagtacca gcagataaaa aagtggatgt
     4561 aagagaagtt ttgtaagaaa ggaagaacct caatgattgt acaagcagtc ttattaggaa
     4621 ttgttgcatt tgtcgcacaa tcagaatatg cactagggac atcgttgctt tctcgtccga
     4681 ttgtgacggg gctgtttaca ggaatcgtgt taggtgatgt aaaaactggg atcattatgg
     4741 gcgcaacttt agagttagca tttatcggat cgttctcagt aggtgcttcg attccacctg
     4801 atgttgtgac cggtgggatt ttagggacgg cttttgctat tacagctgga gccggtacag
     4861 aaaccgcgct tttactaggt ttgccgattg ctacgttgac attgatattg aaaaatattt
     4921 acttagggct tttgattcca attatgaacc ataaagcaga caattatgca gaagaaggaa
     4981 attataaagg agtcgagcat atgcacttgc ttgccggtat cggtctttcc ttgatgcttg
     5041 gtgttgtggt aatggtttct tatatggtgg gaagcaatac gatcggaaat ctattaaata
     5101 tgattcctga cttcgttcaa aaggggttgt cagtagctac agggttgatt ccagctcttg
     5161 gttttgcgat gcttgctcgt ttgctgatca acaaacaagt tgcaccatat tttttcttag
     5221 gttttgctat agcttcttac ttagagattc ctgtgaccgg agtagctatt tttggtgcga
     5281 ttttagcagt tgtggtagtc aatattatga atgtccgtca actaccactt caaacaactg
     5341 caggggaggt agaagaagat gaagaattct gagatcaaaa acagcaaaat aacgaaaaaa
     5401 gagctaaatc acgtattttg gcgttctttt caaatggagt tttcttggaa ttatgaacgt
     5461 cagatgaata tggcttatgt ttatgcaatg attccgattt tgaaaaagtt atatcagtcg
     5521 aaagaagaaa tggcagcagc tttgaaacgg catctagagt tttttaacac gaccccacat
     5581 atcgttacac tgattttagg aatcacggct gcaatggagg aagaaaataa ggatgatcct
     5641 gaatttgatg tgactgcgat cgatagtatc aaaacatctt tgatgggacc tcttgcaggg
     5701 ttaggagatt ctttcttttg gggaactttg cgattgattg ccacaggtgt gggtacctct
     5761 ttagccatgc aaggaaatat tttaggccct atcttgttca ttttgatttt caatattccg
     5821 cacattctgt ttcgctatct agcaacaggt tggggatatc gcttaggaac gggttttttg
     5881 aaaaagatcc aggcaaacgg tatgatggaa agtttgacat taggcgcgtc tattattgga
     5941 ctgatggtcg ttggagcaat gactgccacg atgatcacca tcaatattcc tttaaaaatc
     6001 ggttcaggag aaaatgctgt gactgttcaa agtatttttg atgatatcgt tccaaatatc
     6061 ttatgtcttg gagcatttgg catagtgttt tatctattga agaaagaagt gaaaccatta
     6121 acgatcttga tcggactagc tttctttgga atttttggtt cgtggatagg gattttttaa
     6181 aaaaggagca gagaaaatgt ttacgatgca agattatatc tatgaagaaa aggaagtact
     6241 aagtacgatc ttgaaaaaga atgatttttc gacaagagaa catatgaaaa aaacagtgaa
     6301 tttactgatc ttagcaacgg gttcatctta taacgcttgt ttagcagcga agccagcatt
     6361 ggaatcttat ggtgatctta ctgtcgatat tcaagaacct ttttattttt tcaattatgg
     6421 aaaactttct ccttcaatcg atactgtgat tgctgtgtca caaagcggga aaagtgcttc
     6481 tactgttgaa gcagtaaaaa tgatacaaaa acaagggtta cctgttgttg ctatcacaaa
     6541 tgacgtgcaa agtccacttg ctttagaagc tgcacaaatt attgatttag gtgccggggt
     6601 agaatcagta ggcttcgtta caaaaggcta ttctgcgact gttttacagt tactactctt
     6661 agggatagga attggaataa gtaaaaataa aatcagcaaa aaaatagaac aagactacat
     6721 gcaacagttg cgcaaaataa tcaaccattt accagcgatt attcaaaaaa cggaagaatt
     6781 ttttgatgga tatcaaagtt tatttcgttt agcacagcgt ttcgtcacca ttggatatgg
     6841 tccaaactgg ggaacagcta aagaagcaga gacgaaattg actgaaacaa tccgcgttcc
     6901 gtcacaagga tttgagttag aagcttatat gcacggccca tacctggagg cagatgcttc
     6961 gcatttgcta ttctttattg aaggggaaag tgtaaataag gaacgttcgc aaaagttgca
     7021 aagatatatg agtcggtacg ttggagagac tctcaccatt accacaaaaa aagcaagaaa
     7081 cgagaaaaca cttggattag caatagaatg tgatgaatat ctgtctgtat tagcgcttgt
     7141 cgttccgttt caactattcg cttataaaac cgcagtagct aaaggtattg acttgaacaa
     7201 aaagattttt gaagactttg atacagtctt gaaaagtaaa ttataagagg tggaaatcat
     7261 gctgaaattc aatgaagaag aacaaatcaa agcaataaaa ggcgcactag ctttacgtcc
     7321 gcaagtagaa gaaatcatcg ataaacttta tgcggaaaaa tttgacgctg tctattatct
     7381 aggaatcggt ggaacgtatg cttcctcgat gcaagctgtc acttatatga atgggaaaag
     7441 caacttgcct gtctttgtac agcatgctgc agaatattac actactggaa acaaaagatt
     7501 aacaaaagat tctatcgtta ttttgtcctc tgttaccggt acaactcaag aagtagtgaa
     7561 agcagtagaa caaatcaaga aagtaggagc gacactgatt ggatttatcg ataaagcgaa
     7621 cagtaagttg tctcagctat gcgattttgt ggtcacttat ccagctcctg gaacagaaca
     7681 aatcaaattt tttatggcag cagatcgatt gatgtatttg aatggagaat tcgaagctta
     7741 ttcagaatac tatgaacagc tcgaacgtta tttaccaaca gggttagtgg aagcagagaa
     7801 aaaagcagat gcatttggct tatcatttgc agaaaaacat cgacgtgata gtatgcatta
     7861 ttttattgga gcagggaatc aatggggagc tgtttattca tatgccatgt gttactggga
     7921 agaacaaagc tggcttccat cgaaatctat ccatgctgcc gagtttcttc acgggacgtt
     7981 agagatcgta gaagaaacaa ccccagtcac attattttta ggcgaagatg aacagagagt
     8041 gttatctgaa cgtgtggcta aacttttgcc aaaaatttgc agcaattata cattgataga
     8101 cacaaaagac tatccagtag aaggtatcag tgaaaaatat cgcggacgtg ttttgtcttt
     8161 cttattgatg catgtagtga cacaacgcat cgatgctcat gtggagcagt tgaactgcca
     8221 tccattagag atacgccgct actatcgtca gtttgattat taggatagac tggataaaaa
     8281 gaatataaaa aataaagaga gcagcgataa ctgctccctt tattttttta aaatattggc
     8341 atcaattacg ttataaaaca cattttttgc attatctaca gttcaactta taatctgttt
     8401 aatgggacat gtgggtgcca accatttttt gttatgcaat agtactattt agttgttgca
     8461 tggatccgta gtaaaagtcc aagtacaacg agttgcgcca gtttacatag cgttttgtat
     8521 ccagaatgtt gactaaaaca aaagttccta tgtcaaccgt caacagagac aatctgtccg
     8581 ttgcaggacc tgacagattt agaaatgtcc aatctgtctc ttatgtattt taaatcaata
     8641 ctcactagga gaagtttcct aacctcctaa tagtaatgct ggtagtaaca ttgtcaaaac
     8701 tgcttcctct taggaacaag caaagcataa cgagtgtaaa aataaaatgt agactattgt
     8761 aacatatcat tgattatttc aagtaaaaac gataaaagaa gtaaacaaac acttaaaaac
     8821 aatggaaatt agtcccaact ttaagaaatg tatactataa tggaaattat caattattta
     8881 tttggaggaa tatccatgaa acaaaaaatc gcagttcttg gtcctggatc ttggggcact
     8941 gctttagcac aaacattggc agaaaacggg catgatgtcc gtatttgggg aaatgtacct
     9001 gaacagattg atgaaatcaa taagtatcat acaaatcagc attttttacc tgatttgacg
     9061 atcccagaat cgattatcgg atataaagaa ttggcagaag cagtggataa cgcagatgct
     9121 attttatttg ttgttccaac aaaagcgatt cgttcagtcg cacaggaact tattggtaaa
     9181 atgaatacta agccaatcat tatacatgca agtaaaggac tagaacaaga tacgcataaa
     9241 cgaatctcag aagttttggc tgaagaaatt ccagaagaaa aaagacaggc aattgttgta
     9301 ttatctggtc ctagccatgc tgaggaagtg gcagtacatg atatcacaac aattacagct
     9361 gcaagtattg atcaaaaagc agcagcctat gtacaaaaac tgttcatgaa tgattatttc
     9421 aggatttata caaataatga tgtgattggc gtagaaactg gtgcagcact gaaaaatatc
     9481 attgcaattg gtgccggcgc tatccatgga ttaggattcg gagatgatgc aaaagcagca
     9541 atcatgactc gaggattggc agaaatcagc cgtcttggtg tcgcgatggg tgccaatcct
     9601 ctgacattta tcggtttgag tggtgtgggg gacttgatcg ttacttgtac aagtgttcat
     9661 tctcgcaatt ggcgtgccgg taacttgtta ggacagggac ataagctgga agaagttcta
     9721 gaaaacatgg gaatggtcgt tgagggtgtg gcaacgacaa aagcagcagt agagctagct
     9781 caacagctag gtgttgagat gccaattact caaacaatct ataatgtttt gtataatgga
     9841 gaagatatca aaaaagcagc aaaagaaatc atgttacgtg acggaaaaat ggagaatgaa
     9901 ttttctcttc gttaaataga tgatcaatag ttaatcgtcg t
//
LOCUS       DACDIE010000083         9808 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.083, whole genome shotgun sequence.
ACCESSION   DACDIE010000083 DACDIE010000000
VERSION     DACDIE010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9808
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.083"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(31..2151)
                     /locus_tag="IV250_002449"
     CDS             complement(31..2151)
                     /locus_tag="IV250_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP8769694.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2381..3559
                     /locus_tag="IV250_002450"
     CDS             2381..3559
                     /locus_tag="IV250_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAP8769695.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3575..4333
                     /gene="aroD"
                     /locus_tag="IV250_002451"
     CDS             3575..4333
                     /gene="aroD"
                     /locus_tag="IV250_002451"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAP8769696.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFFSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4356..4841
                     /gene="ybaK"
                     /locus_tag="IV250_002452"
     CDS             4356..4841
                     /gene="ybaK"
                     /locus_tag="IV250_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAP8769697.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4912..6165)
                     /locus_tag="IV250_002453"
     CDS             complement(4912..6165)
                     /locus_tag="IV250_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP8769698.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6282..7631
                     /gene="gdhA"
                     /locus_tag="IV250_002454"
     CDS             6282..7631
                     /gene="gdhA"
                     /locus_tag="IV250_002454"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAP8769699.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7743..9089
                     /locus_tag="IV250_002455"
     CDS             7743..9089
                     /locus_tag="IV250_002455"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAP8769700.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9397..9783)
                     /locus_tag="IV250_002456"
     CDS             complement(9397..9783)
                     /locus_tag="IV250_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP8769701.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         3126 a         1782 c         2009 g         2891 t
ORIGIN      
        1 tttgtcgctg ctccttttaa atttatttta tcattcggaa gaacttgtgg tcgttccgtg
       61 aagttcttgg tacgtttgcg ttttataaag atcaacagtt gattgtccgt tgtgttcact
      121 aaagacacta gttgacttgt caccaagaga tttttcagct tcttttaatt tctctaagcc
      181 attcttgtag tcataatcac tggtatcaat tgggtctaat ccactattcg tatagaaccg
      241 aagaagatca ccattattga tttggtcaga aacagctaac tgtttatcaa ctttttcttt
      301 cagtgaatca atttcattct gtacttcctc agtaggttca gtaatagctt caccagtttc
      361 gttactatag atcgtaccac cgtagtaagt atattttggt gtaacaaaat caccatcacg
      421 gaaagaaaca agctgttcat gatctttcga gaatagatct tgtcctaact gaatataatt
      481 ttttgtatct acacctagta agtgaagtaa tgtaggcaag gcatcaactt gtccgccgta
      541 agtgtgattg attccgccct gtgtttgtcc agggatatgg atcatataag ggacacgctg
      601 catttgtgca ttgtcgtagt ttgtccaagt agaacttgtt tttccaacta attcagctag
      661 atttttattt cttgaattcg agacaccgta atggtcaccg taaaggacga tgactgaatt
      721 atcatacaag ccgctttctt tcaaataatt gaagaattct tgtacggcgg tatctaaata
      781 attggctgtc gcaaagtaac cgttgatcgt ttcatcattt gtattggcta acgggaatcc
      841 tgcttcgtca ttggtgaatt ttgaataagg ataatggtta gatacagcaa tgaattttga
      901 ataaaatggt tgttgcagac gttccaagta ttgtacggat tgttggaaaa atggtttatc
      961 atgcaacccg tattggaacg aattgtcatc attaacatca taataagaag aatcaaagaa
     1021 ataatcatat cctaaattct tgtatgtctc gtttcgattc cagaatgtac cagcgttccc
     1081 atggaatacc gcactaatgt aaccttgtgt ttgacttaaa atatcaggtg cagcttggaa
     1141 agtatttttt ccgcctaatt gtgtgaacaa ggagccttga ttcaagccaa acaatgaatt
     1201 ttccatcagc gtttccgcat cgcttgtttt tcctgctttt acttgatgga aaaagttgtc
     1261 aaaactgaac gtgctgttgc tgtggtaaag actattgatg aaaggtgtaa cttcatgctg
     1321 gagaccattc tcatctgtca atttataatc gatcaggaat tgctggaagc tttctaaatg
     1381 gatataaatc acattttttc ctctagcgag cccgaacaaa tcgtcatttg gtgctgcata
     1441 atgttctttt acataatttt ctacttcttt catatcattg ggacttgctt ctgcccgcac
     1501 ttggtttgtc tggtaagttt gaacaccgtc ataaaccatg aaagcattca agccaagata
     1561 tttgaccaaa taatcacgag agaatgttcg agtcagcaat tctggacgat cagcttccgc
     1621 cataaaaaga tttcctgaga aaatcataac tgctaatgta gagatcgcaa aagccattct
     1681 tgctcggatc gggcgagggt ccaacttgat tttttttgca atcagcagaa cagtcaagat
     1741 aataaaatcg atccaataga tcacatcata tgggcgaaac agtcgaatgg cactttctcc
     1801 cagaccactc gctactttcc cagcacctaa aatcgtgttg atcgtaatga agtccgtgaa
     1861 ttctcgataa taagtgacat tcgaaaaaac aagcaatgtc aaaagaaaat aaatgaccat
     1921 catcgtgata taagcaactc tagggcgtct gatataaaga gcaattccaa gtaataacaa
     1981 tgtagttgca atagggttga taaacaagat aaagtattga atgccacttt cgattccgag
     2041 atggaaatca actaaatagg caaaaatcgt tttcaaccaa agtaaaacga ctagtaaaga
     2101 aaaaaatccg agcctcttat tcaaaaaggc aggtgttttt atacatttca agataatctt
     2161 cctttctgca cttcttaatc acagaagtgt cctgaaacca tgttacacag ctcacttatt
     2221 ctcatttatt ttactagtat gacaatatag tgtcaattga aaccagaagt ttatgctgtt
     2281 tcattaaggt tttctttaag cttcatgaaa aaatgagcag taagacaagt cctatgtttt
     2341 ttgttatact tgggaagaga aagctaaagc aggtgaagga atgaaagtaa aagtaaaaaa
     2401 aagagcaagc aatcgattga aaaaaggata tccactgatc caaaaagaag accttgctca
     2461 tccccaaaaa gaaaccacgc aatgggtgga attagttgat tcgcaaaacc aatttatcgc
     2521 aaaaggatat ctaggaaagc agaataaggg aatcggttgg gtattgagcc agcagaacat
     2581 cccatttgat caatcttttt ttgaagaatt ttttgtgaag tcaaaagaaa aaagaacttt
     2641 ctttttttca gatgattcta ctaccgcttt tcgtatattc aatggagaag gagacgggat
     2701 cggcggtctg attgttgatt attatgcagg ttatgctgtt ttttcttggt ataacgaaac
     2761 gttatatgca catcgtgaat tattgattca agcttttcgt gaaatctacc cggaaatcat
     2821 aggaggctat gagaaaattc gatttgaatc agtaaatctg cctgagagcc agttcattta
     2881 cggaaaagag gcaccggaac ccttgatcgt aaaagaaaac ggagtaaaat ttgccgttta
     2941 tttaaatgaa ggattaatga ctgggatctt tttagaccaa aaagaagtac ggggatcatt
     3001 agcagaaggt ttcgcttctg ggaaaacagt tttgaacatg tttagttata ccggcgcctt
     3061 ttctgttgct gccgcaatgg gaggggcaat cgaaacaaca agtgtcgatc tagcaaaacg
     3121 cagtttaaaa aaaacacagg aacagtttga agtgaatggt ttggatgtcg atcagcaaaa
     3181 aatcattgtt atggacgtat tcgagtattt caaatacgcc aaaagaaaac aatttttata
     3241 tgacatgatt gtattggatc caccaagttt tgccagaaat aagaaaaaag tctttcgcgt
     3301 agcaaaagat tatggggaac tggtcaagga tagtctggat attttagcag aagacgggat
     3361 acttattgcg tctacaaatg ctgcgaatgt ctcgatagaa caattccaaa caatgattga
     3421 agaggaattc catgccaaaa atgtttcttt tgaacaactt gctttatatc gtctgccaaa
     3481 agatttccag acgacggata cctttttaga aggcaattat ctgaaagtgt ttgtttatca
     3541 aattaaaaat aaccattaga aggagcgatg cagaatggtt cgggtaaagg gattgaatat
     3601 aggaagcggt agaccaaaga tttgtgcgcc tcttatcggg gccgtccgag aggaagtatt
     3661 acgtgaagca gttcttgcac gtgaagcagg ggccgattta gtcgaatggc gagtcgatca
     3721 ttatcgtgaa gttttttctt ttgatgaagt agcagaaaca ttggttctta tccatgagat
     3781 gcttgatgga ctgccgttat tatttacgtt tcgtacctta gctgaaggcg gagaacaaga
     3841 catatcgatc cgtcagtatc gcgaccttta tgaacatgtg atacacacag gattagtaga
     3901 tatggtcgat atcgagttgt tcaaagtaga aagtctagga aaaagactga tagaggaaat
     3961 caagtcttta aacattcctt taattatcag tagtcatgat tttaaagaaa caccagcaga
     4021 ccctgtcttg ctttaccgat tgaatgttat ggaacatttt ggagccgata tcggaaaact
     4081 ggcagtaact cccaataatg aacgagatgt tttgcggttg atggagttga ctagacgggc
     4141 aaatgctttt ttctccatgc ctattattac gatgtccatg ggaaatcttg gtaaaatttc
     4201 tcgtctggtt ggcggaacga cgggttccgt tatgacattt ggttccttaa gcagcgattc
     4261 tacttctgct ccaggacaat taccggtcaa agcattgaag caaattattc aattattaaa
     4321 tgaaccgttg tagaacaagt ttgaggtgat attttttggc aaagaaaaaa atagtaaaaa
     4381 cgaatgcaat ccgtatggtc gaacaaaaaa agattcccta tacagagcac gaatatgagt
     4441 gggacgaaag ccacctaagt gcgtcatcgg tcgctgaaca gcttccagaa agccaatctc
     4501 gtatatttaa aacactggtt gctgtgggca atgtgacggg gcctcttgtt gctgtgatac
     4561 cgggagaagc cgaattgaat ctaaaaaaat tagcaaaagt ttcagggaat aaaaaagtag
     4621 aaatgctcca tttaaaagat ttggaagcaa cgaccggata catacgtggt ggctgctcgc
     4681 ctataggtat gaaaaaacta tttcctactt atttagatca aattgcagaa agctatgaac
     4741 aaatcattgt ctcggctggc agaagagggc tacagatgga gttagcacct caagatatct
     4801 gtgcattaac gagcggacaa tttgcagata taaaacaata aatcatacgt ataaaagaaa
     4861 gaaggaggcc gggatgttgt ttgtccagtc tccttctttc ttttttatac attatgctct
     4921 ttgcccggca ttcattcctg ctacatgccc tgtagcgaaa gccgctgtga tattaaagcc
     4981 gccagtataa ccattgacat ccagtaattc tcccgcaaaa tacagaccat caatgatttt
     5041 actctccaat gttttaggat aaatttcctt tagagaaacc cctccgccag ttacaaatga
     5101 tttctcaata ggaaaagttt tgttgattga tagcttaaac tctttgcata gttgaacaaa
     5161 atcttgaatt tgtttttgag aggcttgttg tcctgtcaga tgatccattt caagccgctc
     5221 aagaaagaaa actaacaatc gctcaggcaa tataccatgc caagcattga ccaagttctt
     5281 tttggttgct ttactttttt ctgtcaatat atgaataagc tcttgtttgg tttgtgtagg
     5341 gaaacaatcc agagaaagtg tcactggttc tcccgttttc tctaattctt tattgacaaa
     5401 acttgaacaa cgcagtgcag ccggaccaga aataccgaaa tgggtgaaaa gtaaatccat
     5461 cgtatgttca gtcaatacac gacctttttt atttaaaacg cgtaaagtga tgtcttgtaa
     5521 agaaagccct tgaagtgttt tctcctgaat atacggttca tccgatatca agggggactc
     5581 tgtcgcataa agaggcgtca ccgtgtgacc cactcgcttc acgatcttgt acccatctcc
     5641 agtagaacca gtagatggat aagtccgtcc accagttgtt aagataatgg caggagcatg
     5701 gaactcttcg aattccgtgc gtataccgta aattttatgt tctttgtgga tcaatttctc
     5761 tactcgagta gagaatagaa gcgtcacatc tagttctttc agacgattga gcaatgcttc
     5821 gataatcgtt tttgatttat ttgttacagg aaacatccgt ccatgatcct cttcttttaa
     5881 atgtacgcct tgtgattcaa agaagttcat gatatcgaaa ttattccatt gcgcaaacgt
     5941 actatataaa aactttccat ttccaggaat atgtgcaatc aaatcatcaa caggacgatt
     6001 gttcgtaaca ttgcaccgac caccacctgt catcagtagt ttttttcctg cttttttgtt
     6061 tttttcgatc aagagtacgc gtgcgccttg ttcagctgca ctgatcgctg ccatcatccc
     6121 gcttgttcca gcgccgacta cgatcacatc atacagtttt tccattccct cacctcaagg
     6181 aaagtgtagc atatctgaag aaagaaaaca gaaaaaaatg ttttgagcca aaagagaaaa
     6241 tgagctaaaa tgagggaaga taagcaaagg agcgtggaac gatgacaaac gcaacagaat
     6301 atgttcaagc aattcaagaa aagctacatc aaaaggatca agggcaagaa gaatttttac
     6361 aagcaatcga tgaattcatg cccacggtta tgtctttttt ggacactcat cccgaatata
     6421 tcgaaaagaa tatcttagaa cttttaacgg aaccagaaag agttattcaa tttcgagtgc
     6481 cttggcaaga tgatgaagga aactggcgag taaatagagg gtaccgtatc caatacaact
     6541 cagctatcgg cccttataaa ggtggattgc gcttccatcc tagtgtcaat ctaagtatct
     6601 tgaaattttt ggcttttgaa cagattttca agaatagttt aaccggtctg ccaatcggtg
     6661 gaggaaaagg cggaagtgac ttcgatccta aaggaaaatc tgataatgag atcatgcgtt
     6721 tttgtcaaag tttcatgctg gaattgtcaa aacatatcgg cccatcgatt gatgttcctg
     6781 cgggagatat cggtgtagga gctagagaaa tcggctattt gttcggcgaa tataaacgat
     6841 tgaaaaaata tgatactggt gtgttgacag gtaaaccttt ggatttttgg ggaagtaaga
     6901 tacgtacgga agctactggt tatggattag tgtattatgt caaacatcta ttgaacgaag
     6961 aaaaagattc atttaatcaa aaaacagttt ttgtttccgg aagcgggaat gtagccattt
     7021 atgcaatcga aaaagtgcaa gagctaggcg ggaaagttgt tacatgttca gattctagcg
     7081 ggtacatcta tgatccagaa ggaatagatg tgaaactatt gaaagaagtc aaagaaatca
     7141 atagacaacg tttgacggaa tacgcaaacc aacgtccttc tgctatttat catgctggag
     7201 aatctgtttg gacattgaaa gaaaaagcgg atatcgcttt gccatgtgct actcaaaatg
     7261 aaatagacga aagcttggcc gaaatcttag tagaaaatgg gatcaaaatc gtagcagaag
     7321 gcgctaatat gccatgtaca gtaaaagctg tttctgttct tcatgaagca ggcgtgtggt
     7381 attgtccagg aaaagctgct aatgctggag gagtagcagt atctgcttta gaaatgagtc
     7441 agaacgtcca gcgattgact tgggaaagag agcaagtgga tgagcagctg gatcaaatca
     7501 tggaaacgat ttaccatacg tgtcgccaaa cagcagccga gtacggcgac gaaaaaaatc
     7561 tgcttcttgg agcaaatgtt gctggatttg aaaaagttgc caaagcaatg gcagcagaag
     7621 gtttagtttg aaaaatgaaa ctttcttgga aatattgtca gcattttgtt tatttgcttg
     7681 aaaaataata aaaaaaaacg taaagtatta cctgtagagt aaacctataa ggaggagaac
     7741 aaatggcaca cattcatttt gactattcaa aagtagcacc gtttgtcaac gaacaagagt
     7801 tagggtacat gcaaagccaa gtaacagcag cccacaacga attacgggaa ggtactggcg
     7861 caggaagcga cttccgcggc tggatcgatt taccaacgaa ctatgacaaa gaagaatttg
     7921 cacgtatcaa agaagcagct aaaaaaatcc aatctgattc tgaagtatta gttgttatcg
     7981 gtatcggtgg ttcttattta ggcgctcgtg cagcgatcga cttcttgaat cacacattct
     8041 tcaatttatt agatagcaac acaagaaaag caccacaaat cttctttgcc ggaaattcaa
     8101 tcagctcaac atatattgct gacttgatcg aagtaatcgg tgatcgcgac ttttcagtaa
     8161 acgttatttc aaaatctgga acaactactg aaccagcgat tgcgttccgt gtattcaaag
     8221 agcttcttgt taagaaatat ggacaagaag aagcaaacaa acgtatctac gctacaacag
     8281 ataaagcaaa aggtgcagtt aaagtagaag cagatgcaga aggttgggaa actttcgtga
     8341 ttcctgatga catcggcgga cgtttctctg tattaactgc tgttggtttg ttaccaatcg
     8401 ctgttagtgg tgcagatatc gatgcattga tgcaaggtgc cgctgatgca agcaaagcat
     8461 actcaagcga caaactagaa gaaaacgaag cttaccaata cgcagcaatg cgcaacattc
     8521 tttaccgtaa aggcaaagta actgagttgt tgatcaacta tgaaccagga atgcaatact
     8581 tctcagaatg gtggaaacaa ttgtatggtg aatctgaagg aaaagaccaa aaaggcatct
     8641 atccttcaag cgcaaacttc tcaactgatt tgcactcttt aggacaattc atccaagaag
     8701 gacgtcgcaa tatttttgaa acagttgtga aggttgaaaa acctcgtaaa tcaatcacaa
     8761 ttcctgaaca agcagaagat ttagacggtc taggctactt gcaaggaaaa gaagtggact
     8821 tcgtaaatac aaaagcattt gaaggaacat tgcttgctca tacagacggt gacgtgccta
     8881 acttgctagt caaaattcca gaaatggatg cttatacatt gggttataca atgtacttct
     8941 ttgaaattgc tgtaggtatt tcaggttact tgaatggaat caacccattc gaccaaccag
     9001 gtgtagaagc ttacaagaaa aacatgttcg cacttctagg caaaccaggc tttgaagatc
     9061 ttgctaaaga attgaacgaa cgcctgtaag ataagggttt ttcgctagat ttaaataatc
     9121 agtgatagaa acatgttcgc tttaaaatga aaactggtac ccatttaggt actcaaacat
     9181 actgcatcgt ttattcagaa taaggggctg tgacaagacg tttgtcacag tcccttgttt
     9241 gtatagaaat cgattgattg ccttgctctt tagtttgttt taggacttta agaacagtga
     9301 ggctgacttc gaaggttaat tctgtttttt tgttttctag aatcagtaag caaaaaagca
     9361 ctcacttcga gtgctttttt gcgatatatt cttagcttaa gataggccta atttttcttt
     9421 tacagatgag ggtaccttat cgctggtaat tacttttttc tctatgatcc gcttttcgct
     9481 tgctttcaac tgcaagatcg ttccttcggg gtaagcagaa ccattggcag agtcaccaga
     9541 agtaatgaca tcgaaatcta actgtctttt ttcaccgttt tcagcataag catctacgtt
     9601 gtatttaaaa cctcttccaa ttacttcccc attatctgct ttgatatcag tttctgcagg
     9661 cattggagct ttgactaccg cataataatc tttacctaca taagtagact gatagtagtc
     9721 ccaacctttc catcctataa aagcaatcaa tatgataacg atcaacccta aaattttttt
     9781 catgaaagat ctcctttttt atttttat
//
LOCUS       DACDIE010000084         9453 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.084, whole genome shotgun sequence.
ACCESSION   DACDIE010000084 DACDIE010000000
VERSION     DACDIE010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.084"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..567)
                     /locus_tag="IV250_002457"
     CDS             complement(<1..567)
                     /locus_tag="IV250_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP8769702.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDG"
     gene            833..1612
                     /locus_tag="IV250_002458"
     CDS             833..1612
                     /locus_tag="IV250_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769703.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTVLSQIEQIRNEKIRAAYATKAQTVAQQMGYSNISY"
     gene            1658..2602
                     /locus_tag="IV250_002459"
     CDS             1658..2602
                     /locus_tag="IV250_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8769704.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFTIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            2618..3397
                     /locus_tag="IV250_002460"
     CDS             2618..3397
                     /locus_tag="IV250_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HAP8769705.1"
                     /translation="MEETISLKEIFETLRKHLVTIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            3409..4107
                     /locus_tag="IV250_002461"
     CDS             3409..4107
                     /locus_tag="IV250_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HAP8769706.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAS
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQVLAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENMSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            4135..4899
                     /locus_tag="IV250_002462"
     CDS             4135..4899
                     /locus_tag="IV250_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAP8769707.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPIYEEVKKKKFGLF"
     gene            4912..6729
                     /locus_tag="IV250_002463"
     CDS             4912..6729
                     /locus_tag="IV250_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008378780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP8769708.1"
                     /translation="MFVLTRRRKIATLVFVDSLLLIAANIAAAKFMKPFVAIPMDLIL
                     ISIGLSIGFYLFYGSLFKVFTRINRYTNLREIIAVFGSLSASAVSSIIILLFINRRYS
                     LRLVIFAYLLSLLLIIGSRLIWRIYVETKNMRYLSTDSAKNTLIVGAGEGGRILYNSF
                     LGSKTAQDIHVVGFVDDDPNKRNTYLSGKKVLGALKDIPELIEKYDIQMVTIAIPSLS
                     RKKLRRIFELVESARVKVNTMPSIEELASGKISVSKLKTIDVVDLLGRDEVKLDIESI
                     KDQITDKVILVTGAGGSIGSEICRQIIQFNPAKLLLLGHGENSIYLIDRELRTHHQNC
                     PTEIVPIIADIQDREKINEIMEQYHPDIVYHAAAHKHVPLMEYNPKEAVKNNIFGTKN
                     VAEAAKAARVKNFVMVSTDKANNPPNVMGSTKRIAEMIVTGLNEEGCTKFSAVRFGNV
                     LGSRGSVIPVFREQIAQGGPITVTDFRMTRYFMTIPEASRLVIQSGALAKGGEIFILD
                     MSEPVKIVDLAKNMIRLSGYSEDEIEIIETGIRPGEKLYEELLLDKERNDEAVYEKIF
                     VGNIKGYSIQKVMDFVKSLPQDDEQLAKDIVTFANASNK"
     gene            6740..7372
                     /locus_tag="IV250_002464"
     CDS             6740..7372
                     /locus_tag="IV250_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP8769709.1"
                     /translation="MKAIIVGAGGFGKEIAFLLQSVSRYELVGFVDDSLKMQNQELLE
                     KPILGTIDLLIELEEETAIFLGIASPDIKEKIYQKINKNNKLIFPNLVAPSALVGINV
                     QLGIGNILMPYTTYTADVVLGNFNMINIGSTIGHDTQIGNYNSVFPSVNISGHVILGD
                     KNEIGVGTKIIQNLSIGNTNIIGAGSVVIKNINNNTKNVGVPTKVIERWD"
     gene            7374..8492
                     /locus_tag="IV250_002465"
     CDS             7374..8492
                     /locus_tag="IV250_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HAP8769710.1"
                     /translation="MENKRILLASPHMSEEGYEQEYVKEAFDTNWVAPLGANVNGFEK
                     ELAEYVGASHAAALSSGTAAIHLALKAAGVGKDDIVICQSLTFSATANPILYQGAIPV
                     FVDSDYETWNMKPEHLRTALEKYPNTKAVLVVHLYGLSANLDEITKICKEYDVTLIED
                     AAESLGTTYKGKYTGTFGDYGIYSFNGNKIITTSGGGMLVSNEKEKVEKVRFWATQSR
                     EKARHYQHNEIGYNYRMSNIVAGIGRGQLKVLNERIEKKRYIHNFYKKELSKLSNIQF
                     MPENNWNEPNYWLSVITIKGINTNHLIDELDKNNIEARPVWKPMHLQPVFKNYDYIGD
                     DVAETLFDLGVCLPSDTKMTDEDLKYVVSTIKRVINDA"
     gene            8485..9102
                     /locus_tag="IV250_002466"
     CDS             8485..9102
                     /locus_tag="IV250_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP8769711.1"
                     /translation="MRKKTFYQLFGKRILDILLSGIALIVLSPIILIVGFLVRIKLGS
                     PIIFKQERPGKSEKIFSMYKFRTMTDERDHNGEYLPDEIRLTKFGKILRATSLDELPE
                     LWNILKGDMSIVGPRPLLVEYLPLYSEKQRKRHNVRPGLTGLAQVNGRNAISWEEKFD
                     LDVYYVDKISFFNDLIIIIQTCKKVIKKENINTINNNIPEKFKGS"
     gene            9110..>9453
                     /locus_tag="IV250_002467"
     CDS             9110..>9453
                     /locus_tag="IV250_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP8769712.1"
                     /translation="MPLNILFLTLLDFQSLEESNIYTDLMSEFVKNNHNVSIISPTER
                     KKRIKTHLIEINDKCEILKLRIGNIQKTNLIEKGFSTIQIERKFISGINQYFSNKKFD
                     LVIYSTPPVTFA"
BASE COUNT         3353 a         1572 c         1884 g         2644 t
ORIGIN      
        1 tccatctaat cgaatgccaa ttacaatata aactgcttct tttgaaacag tttgtctttt
       61 taaaggaatg tgagtagcat ccataaaaat gacagagtat ctggattcta aggacctttc
      121 tttaaaggca ataatatctt cagaaactat tttagtcata tttgaaatgg tttgtggcgt
      181 atagtgatgc ccgtacattt tttcaatcaa ttcagagatt tcagacatag taattccttt
      241 ttgaaatagc tgaataatag tggtttctaa agagtcattg gttcttttat aagcaggtaa
      301 agtttgttga gaaaacttac catttctatc tctaggaatc accaaattta attctccata
      361 ctctgttctg aatgaacgtg aataattccc attacgggaa ttacctgaat tgaacccagc
      421 acgatcatat ttttcataat caagaaaagc tgttaattca gcctgcaata atgaattaac
      481 agctaattct aaatgacatc gaaataattc atctaaatca cctttattga ttagtgtttc
      541 cataatttct gtagtaaaat tgggcatgag aaagtcctcc tataaaattt cggttgtggt
      601 aactttaatt ttacagaatg gactttcttt ttttctaccc taaatatcta tttacacaaa
      661 atattttacg ctatcaaaac agtttgtggt atgattatat agggaaaact tgtttacaaa
      721 tatttctgtg aatatattag tttaattatg ttttttaaaa taaaaagctg tcatttttat
      781 gtaagtgaat tatatcttaa tttatctatc atcaatactt aggagtgaat ctatggaaaa
      841 gccattcgtc aagtcattga ctgctgtggc ggtaggtgca ggtgctattg cgatctgttt
      901 gtttggttac catatcaata atcagcgaca acatcagcaa cggatcaatt atgcagaatc
      961 tgccatcacc aatcaaaaag acaccttgac ttctttatca aaagaagtag acaagctgta
     1021 tagtacaaaa gaaaaaattt ttctaaaccc tgaaataaca gaagaaacaa tcacaagttt
     1081 gtcaaataag ctatcctctg tcaaactttc agcagatgat tacgacatca aagaatcaga
     1141 attgcctaaa gaagctgttg caatccaaga tgagaagaaa gcaatcgtga cacagttaga
     1201 tagtgcagaa tcaaaactga agatccagac agctgtcaac gatttattta ctaaaaatgt
     1261 atcaaactgg cagcaagctg tagacgatgt catcatcaaa gaaaaggtga caagtgcaga
     1321 tgtcgctcgt gtacgtgaaa atatgagctt ttttaaagat agtccttgga aaacggttgt
     1381 tatgcaatat ctaggattcg ctgatactca gatcgcgcaa gtcacgcaac tagatcagtt
     1441 attcgatacg atgttgaagg atggtcaagt gactgccacc gctacttacg atcaatattt
     1501 gacggtgctc agtcaaatag agcagatccg gaatgaaaaa atacgcgcag catacgcaac
     1561 aaaagcacaa actgttgctc agcaaatggg ctatagcaat ataagctatt gacagttaaa
     1621 acgttgattc aagcaaggtt agcaaacgga ggaaactatg gcaagacgaa gaagaaaatc
     1681 aagagggaaa aaaatatttc tgattatttt agcagttctt atcgtattag tattagcaat
     1741 tgtaggagta ggagcaaaat tatatctgga tctctcacat tctattgaga aaacttacga
     1801 atcagtggaa cgaacccaag gagaacgaac gaaaccagtt gatctggaca aacaagaatc
     1861 tttctctgtt ctattgatgg ggattgatac tggagattta ggtcgggtcg atcaaggaag
     1921 atcagatacg atgatggttg ccacagtcag tcctgaagac aaacagacaa cgatcgtcag
     1981 cattcctcga gatacgtatg tcgacatcgt aggtcgtgac acaaaagaca agatcaacca
     2041 cgcttacgca ttcggcggag ctgccatggc gatggatacc gtacaaaaat atgtagacat
     2101 cccaatcgat cactatgtct ctatcaacat gaaagggcta aaagaacttg ttgatgcagt
     2161 tggtggtatc gaagtcaata atgacctcac tttctcacaa gatggctatg actttacaat
     2221 cggtaaaatt actttgaacg gggatcaggc attatcttat tctcgtatgc gttatgaaga
     2281 tccaaacggt gactatggta gacaagagcg tcaaagaaag atcgtagaag gcgtagcaaa
     2341 aaaagtattg agtctaaacg gtgtcagcaa ctatcaatcc gttttgaatg ctctagagtc
     2401 gaatatgaaa acagacatga gctttgatga aatgaaaaaa attgctttag attatcggga
     2461 ggcatttggt acgatcaagc aagatcagct gcaaggagaa ggcttcatgc aagataatat
     2521 ctcctatcaa aaagtcagtg atcaagaact tgatcgtgta aaaaaagaat taaaagatca
     2581 aatgaatcta gagaataaat aggaaagtgg agaaaaaatg gaagagacaa tcagcttgaa
     2641 agaaattttt gaaacattac gcaaacactt ggtaacaatc atcatctcaa tgtttgctgg
     2701 cttggccatc tcaggaatcg tgacgttttt catcttgaca ccaaaataca gttcacaagc
     2761 acaattgatc gtgacactgc cacaatcaga cacagcaaat gtcaatgatg tcaatacgaa
     2821 cctgcaaatg atcaatacgt ataaagatat gatcgtcagt gatttagtac taaatgaagt
     2881 caaagatcgt ttagaaacag aagacaatgt gaaaatgact gctggacaaa tcaaagatgc
     2941 gatctccgtc aatcagtcac aaaattcaca aatgttctcg atccaggcaa taagcacgaa
     3001 tgcagtgact gctcaacaga tcgctaacac aacttcacaa gttttccaag aaaacgccaa
     3061 agacgtgatg aatgtcgata aaatctcgat tatttcaggt gcagttgcca atacgacacc
     3121 tgtttcgcca aataataaat tgaatctagt gattggctta gtgttaggaa tgatggtagg
     3181 cgtaggtttg gcattcttat tcgaattact tgatcggaca gtgaaagatg aaaaatatgt
     3241 gacggataca ttaggattcc caatcttagg aacagttcca gaaatgtcag cgaaagaatt
     3301 gaacgctggt atccgtaaaa aaggaacagc tcctgcacga ccaaaagcta caaacgataa
     3361 aacagcccca tcacgcagaa gccgctcaag agtctaggag gaagaaaaat ggcacgaaca
     3421 cagaaacaaa aagcagcaca aactcatgca gtcagtttga tcacattaac aaatccatct
     3481 tcaccaatcg ccgaacaata tcgtacgatc cggacgaata tccagtttgc ctccgcagct
     3541 ggtcagcaaa tcaaaacgat tgtcgtgact tcatcaggtc ctggagaagg gaaatccacc
     3601 actgcagcaa atattgcagt cgtttttgct aaatcaggtc aacgagttct actagtcgat
     3661 gcagatttac gtaaacccgt cgtctataag acattccagc tgaacaatgc cagtggatta
     3721 agtacagcac ttagttcatc aggaagtgta gcagatgtga tccaacgaac gccagtcgag
     3781 aacttgtcaa ttttgcctag tggacctaag ccacccaacc catcagaatt gttaagctcg
     3841 ccaagaatgg atcaggttct agccgaagca agacagttat ttgacgtggt gatattcgat
     3901 atgccaccag tagtagccgt aacagatgca cagatcatgt cttcaaaaac ggatggcact
     3961 ttattggtcg tacgtgaaaa tatgtcacgt aaagaatcct tgactaaagc aaaagaactg
     4021 ctggagatgg tacaagcacg agtcttaggt gttgtgtaca acggagcaga acatagcaaa
     4081 gatgctggtt attactacgg aaattaagga ataagaagag aggagacaag agaaatgatc
     4141 gatctacatt gccatatcct accaggaatc gatgacgggg cagaaaactt agaagcaagt
     4201 attgcaatgg ctgaaaaagc gattcagcaa ggcatcacgc acattttatg cacaccacac
     4261 cacaataatg gaaaatacag caatgaaaaa tcacaagtca tttctctagt cgcttcgtta
     4321 caagccgaat tagagaaaag acagttgcct ttgacgttat tggaaggaca agaagtacgt
     4381 attaccggaa cgctcatcga agacatccac cgagatgaaa ttttatttac agatttagat
     4441 gatacatatc ttctgatcga atttccgaca ctggaagtgc cgttatatgc agaacggctt
     4501 ttttttgagt tgttagaact aggaaagaca ccagtgatcg tccatccaga acgtaacgcg
     4561 cattttcgcg aagacccaaa tcatttgctt ccgttcctag atatgggatg tttggcccaa
     4621 ctcactgcgc caagttatgt tggggtattt ggcaaagaca tccagaaaac agccaaaacg
     4681 atggtcaaac ataatcttgt gcagatggct gcttctgatg cccatggggt ctctaagcgc
     4741 accttttact taaaagagtg ctatgaacag attgccaagg actttggtaa ggaaaaagta
     4801 gtacagatga aacaagtcgc aaaggatctg atcaacgggg atgaaatcag ttatccaatc
     4861 tatgaagagg taaaaaagaa gaagtttggt ttattttagg gggaatgtag ggtgtttgta
     4921 ttaacaagaa gaagaaaaat tgccacattg gtatttgtgg acagcttgtt gttgattgct
     4981 gcgaatattg cagcagctaa attcatgaag ccgtttgtag ctataccaat ggaccttatt
     5041 ttaatctcta tcggtttgtc gattggtttt tatttattct atggtagttt attcaaagta
     5101 ttcactcgga tcaatcgcta tacaaattta agagaaatca ttgcggtttt tggctcacta
     5161 tctgcaagtg ctgtctcaag tatcatcata ttgctgttca taaatcggcg atacagttta
     5221 cgtttagtca tttttgctta cctgctttcg ctattattga ttattggtag ccgattgatc
     5281 tggcgtatct atgtcgaaac aaaaaatatg agatatctat ccacagattc tgctaaaaat
     5341 acattgattg tcggtgctgg cgaaggcggg cgtattttat acaacagctt tcttggatcc
     5401 aaaacggctc aagatatcca cgttgtcggg tttgtagatg atgatccaaa taaaagaaat
     5461 acgtatcttt caggaaaaaa agtattaggt gcattaaaag atattccgga attgattgaa
     5521 aaatatgaca ttcaaatggt aacgatcgct atcccttcct tatcaagaaa aaaattacga
     5581 agaatcttcg agttagtcga atccgctcgt gtgaaagtca acacgatgcc ttctatagaa
     5641 gaacttgctt caggaaaaat tagcgtatca aaattaaaaa caattgacgt agtggattta
     5701 ctgggaagag atgaggtaaa gctggatatt gaatccatca aagatcagat cacagacaaa
     5761 gtcattcttg tcaccggcgc aggaggatcg atcggttcag aaatctgtcg gcaaatcatt
     5821 caattcaatc ctgcaaagct attgctttta ggacatgggg agaactcgat ctacttgatt
     5881 gaccgtgagt tacgcacgca tcatcaaaat tgtcctacag aaatcgtacc gatcatcgca
     5941 gatatccaag atcgcgagaa aatcaatgag atcatggaac aatatcatcc agatatcgtc
     6001 taccatgcag ctgctcataa acatgtacca ctgatggaat ataatccaaa agaagcagtc
     6061 aaaaacaata ttttcggtac aaaaaatgta gcagaagcag ccaaagcagc aagagtgaaa
     6121 aacttcgtca tggtatcgac agataaagct aacaatcctc caaacgtcat gggttcaaca
     6181 aaacgaatcg ctgaaatgat cgtgactgga ttgaatgaag aaggctgtac gaaattctca
     6241 gctgtcagat ttgggaatgt ccttggttct cgcggtagtg tgattccagt attcagagaa
     6301 caaattgctc aaggtggtcc aatcactgta acagactttc gtatgacccg atacttcatg
     6361 acgattcctg aagcgagtcg tttagttatt caatctggcg cattagcaaa aggtggcgaa
     6421 atttttattc tagatatgag tgaaccagtg aagattgtag acctagccaa aaacatgatc
     6481 cgattgagcg ggtatagcga agacgaaatc gaaatcattg aaacaggtat ccgtccaggg
     6541 gaaaaactat atgaagaact attgttggat aaggaaagaa atgatgaagc agtttatgag
     6601 aaaatctttg taggaaacat caaaggttac tcaatacaaa aagtgatgga ttttgtcaag
     6661 agcctgccgc aagacgatga acaactggct aaagacattg tcacttttgc aaatgcttca
     6721 aataagtagg gggagtaaag tgaaagcaat tattgtagga gctggtggat ttggcaaaga
     6781 gattgctttt ctattacaaa gtgtctctag atatgaattg gttggttttg tcgatgattc
     6841 gttaaaaatg caaaatcaag aattattgga aaaacctata ttaggaacaa tagacttgtt
     6901 aatagaatta gaagaagaaa ctgctatttt tctgggaatt gcttcgccag atatcaaaga
     6961 gaaaatatat cagaagataa ataaaaataa taaattgatt ttccctaatt tagttgcacc
     7021 aagtgcacta gtgggcataa atgttcagtt aggaattggc aatattttga tgccctatac
     7081 gacttacact gctgacgtag ttttaggaaa tttcaatatg atcaatatcg gctctactat
     7141 cggacatgat actcaaatcg gtaattataa ttcagttttt ccaagtgtaa atatttcagg
     7201 acatgttata ttaggagata aaaatgaaat tggagtaggt actaaaataa tacaaaatct
     7261 aagtattgga aatacgaata ttataggtgc aggaagtgta gtaataaaaa atattaataa
     7321 caatactaaa aatgttggcg tacctaccaa agtaatagaa agatgggatt aagttggaaa
     7381 ataaacgaat attattagca tctcctcata tgagtgagga aggatatgaa caagagtatg
     7441 taaaagaagc atttgataca aattgggtgg ctcctttagg tgcgaatgtg aatggatttg
     7501 agaaagaatt agcagaatac gtgggagcta gtcatgctgc cgcattatct tctggaacag
     7561 cagctataca tttagctttg aaagcggctg gggtcggtaa agatgacata gtgatttgtc
     7621 agtccttgac gttctcagct actgctaatc caattctcta tcagggagct atacctgtat
     7681 ttgttgatag tgattacgaa acttggaaca tgaaacctga acatttgcga acagcgctag
     7741 aaaaatatcc taacacaaag gctgtattag tagtacattt gtacggttta tcagctaact
     7801 tagacgaaat tactaaaata tgcaaagaat atgatgttac tctaattgaa gatgctgcag
     7861 aatcattagg aacaacatat aaaggaaaat atactggtac attcggcgac tatggaattt
     7921 attcttttaa tggaaataag ataatcacaa cttcaggtgg cgggatgctt gtgtctaatg
     7981 aaaaagaaaa ggtggaaaaa gtgagatttt gggctacaca atctcgtgag aaggcacgcc
     8041 attatcaaca taatgaaatt ggctataatt atcgaatgag taatatcgtt gcaggtatcg
     8101 gtcgaggaca attgaaagtt ttaaatgaaa gaattgagaa aaaacgatat attcataatt
     8161 tttataaaaa agaattgtct aaattgtcaa atatacaatt catgccagaa aataattgga
     8221 atgaaccgaa ctattggtta agcgtcataa caataaaggg gataaatacg aatcatctta
     8281 ttgatgagtt agataaaaat aacatagaag cccgcccagt atggaaacct atgcatttac
     8341 aacctgtttt caaaaattat gattatattg gcgatgatgt agcagaaact ttatttgact
     8401 taggagtatg tttaccatca gatacaaaga tgacagatga agatttaaaa tatgtagtat
     8461 ctacgattaa aagagtgatc aatgatgcgt aaaaaaactt tctatcagct ttttgggaaa
     8521 agaattttag atatcctatt gtcaggcatt gctctcatcg ttttatcgcc gataatctta
     8581 attgtaggtt ttttagtaag aattaaactt ggttctccaa ttatcttcaa acaagaacga
     8641 ccaggaaaaa gtgaaaaaat tttttccatg tataaatttc gtacaatgac tgacgaaaga
     8701 gaccacaatg gagaatattt acctgatgaa attcgactaa ctaaatttgg gaaaatactt
     8761 agagcaacaa gtctagatga gctaccagaa ctttggaata ttttgaaggg tgatatgtcg
     8821 atcgtaggac ctcgtccatt attagtagag tatttgccac tatatagtga gaagcaacgg
     8881 aaaagacaca acgtaagacc aggattaact ggactagctc aggtcaatgg aagaaatgcg
     8941 attagttggg aagaaaagtt tgatttagat gtttattatg ttgataaaat tagtttcttt
     9001 aatgatctta ttattataat tcagacatgt aaaaaagtaa tcaagaaaga gaatattaat
     9061 acaatcaata acaatatacc agaaaaattt aaagggagtt aaatatagta tgccattaaa
     9121 tattttattt cttacgcttt tagattttca aagtttagaa gaaagcaata tctatactga
     9181 tttgatgtct gaatttgtaa aaaataatca caatgtgtcc attatatctc caacagaaag
     9241 aaaaaaaaga ataaaaactc atttgatcga gataaatgat aaatgtgaaa tcttaaaatt
     9301 aagaatagga aatattcaaa agactaattt aattgaaaaa ggtttttcta ctatacaaat
     9361 agagagaaaa tttatatcag gcattaatca atatttttca aacaaaaaat ttgatctggt
     9421 tatttattct actccgccag taacttttgc taa
//
LOCUS       DACDIE010000085         9289 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.085, whole genome shotgun sequence.
ACCESSION   DACDIE010000085 DACDIE010000000
VERSION     DACDIE010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9289
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.085"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            14..751
                     /locus_tag="IV250_002468"
     CDS             14..751
                     /locus_tag="IV250_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769713.1"
                     /translation="MADRKLEKLLEETWNPKEFSEFFMENFETDLAVIVKDALREQGY
                     PETANYININFTLYTENKGTWDFWATLANKELSDKSDTGIRNFFESNRDDYMYANHQD
                     KLNFRVEFDETPEEFIERQPPKENVAKVLEDRWNSDEIVSTISELGGQYEPLVEAVRE
                     ELRLNKFPDVQNIDVSQIEINVKITNKLDYGSWADIALEKYIYSTLKEFIENRMDIMY
                     LQHPQYLNFGVEIATPLEEWKMEQGLD"
     gene            complement(768..1184)
                     /locus_tag="IV250_002469"
     CDS             complement(768..1184)
                     /locus_tag="IV250_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769714.1"
                     /translation="MNKKRKLSVLITLVSILTILSGILSLKIFNQINTSSNAKDGIVV
                     ENTSNSKETVTTTKKDKSAEITTKNYLIKTVYFDERKTAILKDEEGSELKLPITVSDK
                     FNGTFSLQTAEGSPDNLKVGQTIILGKNDELYSFVK"
     gene            1576..2823
                     /locus_tag="IV250_002470"
     CDS             1576..2823
                     /locus_tag="IV250_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HAP8769715.1"
                     /translation="MNMKKNSLGMVLFASAVLAGLSVPVTSHADEWTANTPDQIQITT
                     GQSSYAFKKGDTIWSTVEKLKSLGYKTTVAEFAELNNVDLAKGEQRTIPIGRTFNISK
                     DTLTVKEADGEVVSQTVQKDKPDPVENTEQENKKSETAKESEPTANTSDKTETSASSV
                     DGASSSNTNNSTSEKEASQGTSESSTTETDTGTSTNNSQSTSNDANESTSTSSSDQSQ
                     TGSSDETDSSTSPSKPEQPTNVVLGVPFISQGNTMLCEGTSLLEALHYKGVATNQDLM
                     TFVKSMPLSPDNNPNNGFSGEWRHNVDGTYQGMNPAPVVSWGVANGGNVVDISGSSVQ
                     GLKDEIVNGNPVVAWVTYAFEPAQHKSMFWGDAIWNRHVVAVDGFKDGFYHVVDPVFG
                     ASWINAGSFETAYNVSQLAVSVR"
     gene            3093..5483
                     /locus_tag="IV250_002471"
     CDS             3093..5483
                     /locus_tag="IV250_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HAP8769716.1"
                     /translation="MKHEKTIILKSKIIYCFATLVMVLSTLIMPFSAIRAEADVTKPT
                     SAKFTETGVKTDGYIRVNMPNHPNEWMISSQFKDSHGNIGYCMDSELPSPTGSGAGSL
                     KYKGAGSDEFYRMFKGGFPSKTAKELGAGNDTEAWYATQLVSWVLAGNFKVSQIVWSH
                     PNHTAAETARVKKAFEKIYDYAKNGKDTPNTEFSITASKTADEGKYHTFTYKTASNKT
                     GNAKLTFTSAKPAGMKIYDADGKEITNNTVKLNSSFTIKVPVTTPSGTLSFKGTANVS
                     TTNPFTFDGRGVYQDAVVMITTSETKDSKSLSAKWTRAVGQVTVKKVDQDGNALKGAE
                     FTLTDNNNAKSVKTTGDDGLATFDLQQGLTYSLEETKVPEGYQGSFKKDGITVNTDGQ
                     EFSYTATNTIKKGKVKVKKVDQDGKPLANAEFTLTDDSSQTQAEKTDENGLASFDIVA
                     NKTYSLEETGVPTGYTGVFKQEGITVKDDGQTFEYTATNTLKKGLVKVHKVDQDGKPL
                     ANAEFTLTDEKGATQVEKTDENGLASFNIVANKTYSLEETGVPVGYTGAFKQEGITVK
                     DDGQTFEYTATNNLKKGVVKVHKVDQDGKPLADAEFTLTDEKGVKEVKKTDATGLATF
                     NIIANKTYNLEETKVPTGYTGAFKQEGITVKDDGQVFEYTAKNTLKKGVIKVHKVDDQ
                     GKALANAEFTLTDDKGVKVVQKTDSNGLATFNIVANKTYKIEETQNPNGYTGKWSKEG
                     ITVADDEQTFEYTVINHLVPETVRTGGIGTSAFLLVFLIAGLSMVLLYYREQHKAK"
     gene            5532..7367
                     /locus_tag="IV250_002472"
     CDS             5532..7367
                     /locus_tag="IV250_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP8769717.1"
                     /translation="MKSKKIFSNLMLFGTLLGVGLGASSISSSLGLGGQVAYAQQNYN
                     YQGTGTIHIHKVTNTNGDGNPVSDIDNKGKEIALPNGVKPLEGVEFSYVKLTKDEAKS
                     LGWTDSDTTKGQHIDDAKARLLLNKKYSEVSRMKTDSQGNIDIPIQVNTDDLTDNLYL
                     IVETGTVKGVVEEAYPMIVNVPELNGSDDQYFNYDVHVYPKNYFQLNSAELDKKDEDG
                     KALAGTVFELRKDDGSDQVVGTYKTDSTGKLTVDGLELGDYYFLETDLGTDANKTTYL
                     LDGKKFKFSVKRGETAKVVLDVTNYKRPVITKVIDKETVDTGEDVQYTLKPSIPGNIG
                     NYEKYVVKDVLGAELTYDSSEVIEGMTLDTDYSIERDGQTTNYVFTESGMDKLQKQFD
                     AGDRFFTILMNAHTNSKAKMDTPLKNDVSTIYNNGYEPEDETPVDHAYTIIGDHQFIK
                     TDGDTSKALSGAKFVVKNKIDPNKVTYLKQDPTTKAVTWIADKSKATEFVSGSDGKFQ
                     IEGLQYTYDVDFDKQGNPTITDKKINDYALEEVVAPSGYTPTRNDVPFVVSNDDASSE
                     VTDIKNLKTPETVRTGGIGTVVFLVVAGVAGTGLYVVYKREQHKA"
     gene            7442..8299
                     /locus_tag="IV250_002473"
     CDS             7442..8299
                     /locus_tag="IV250_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8769718.1"
                     /translation="MKKKNIKLIILKIGMLLCLGVGLYPVITQAYTYYQTQQTLNSYK
                     EKYDEASEKENEEVFKRYSEVNRKRKDTEQTSVTIPEDNNNIPSNGNLQKDKLGELVG
                     EILIPKINVDLPIYDGVNDFQLSAGAGILTGTDYPTGGIGNHSVISAHSGIPNKKYFT
                     DLELLKLGDKFFIDISGKKLAYQVDRIKIILPTEFEDLLPVAGKDYVTLMTCTPVGDN
                     SHRLLVRGVSIPYQKSIVEKEKKKEDLKVKAQMNWYPIVVVGVLLIIAVIVIIKKIVA
                     KRRGMKNEE"
     gene            8289..>9289
                     /locus_tag="IV250_002474"
     CDS             8289..>9289
                     /locus_tag="IV250_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8769719.1"
                     /translation="MKNKILLFLTLICTLVLTIYPANQLYHQFKTKENVAEIQEKIEK
                     KQTPSKEEVAKKVEQIKSNSGNQGLDNTQKTDGGSFDIGEAEGKVNPYTGDVFSKNQT
                     KDFEPSTYSGAVGYVYIPKINVLELLFLGASQEHLYRGVAQMSATSLPLSGKGHQTVI
                     AGHRGGYTSPQFLYVDQLVAGDHIYIDYLGEKAVYTVFRTDVIRNYGQNSTLTDIPDL
                     DMLTLYTCHPYPQNYDRLLVRSLRDEKAKYITPQVTVGNTAYADAIDSEYVSDKTETV
                     TSSNDLFEDVDRANKELDGNNDLNIPEQGSVEMQDRTFAEKASSIFTGITNKHQKML"
BASE COUNT         3414 a         1439 c         1863 g         2573 t
ORIGIN      
        1 ggggagaata ataatggcag atcgtaaatt agaaaagtta ttagaagaaa cgtggaatcc
       61 aaaagaattt agtgaatttt ttatggagaa ttttgaaact gacttagcag tgattgttaa
      121 agatgctctt agagagcaag ggtatcctga aactgcaaac tatataaaca ttaattttac
      181 tttgtatact gaaaataaag gaacttggga tttttgggct actttagcaa ataaggagtt
      241 aagtgataaa tcagacacag gaattcgtaa cttttttgaa tcaaacagag atgattatat
      301 gtatgctaac catcaagata aattgaactt tagagtagag tttgatgaaa cacctgaaga
      361 atttatagaa agacaaccgc ctaaagaaaa tgtagcaaag gtattggaag atcgttggaa
      421 ttcagatgaa atagtttcaa caattagcga actaggcgga caatatgaac cgctagtaga
      481 agcggttaga gaagagctaa gactgaataa gtttcctgat gttcaaaata ttgatgtttc
      541 tcaaattgaa ataaacgtca agatcacgaa taaacttgat tatggtagtt gggcagatat
      601 agcattagaa aaatacatct actcaacgtt aaaagagttt atcgaaaata gaatggatat
      661 catgtatcta cagcatccac agtatttaaa ctttggtgtt gaaatagcta cacctcttga
      721 agaatggaaa atggagcaag ggcttgacta gcctttgctc cattttttta ttttacaaat
      781 gaatacagtt cgtcgttttt accaagaatg attgtttgcc caactttgag attgtcaggt
      841 gatccttcag cggtttgtaa ggagaacgtt ccgttgaact tatcggatac cgttataggt
      901 agctttaatt ctgatccttc ctcatctttg agaattgctg tttttcgttc atcaaaataa
      961 acagtcttta ttaaataatt tttcgtagtg atctcagcag atttgtcttt ttttgtagta
     1021 gtaacagttt ctttagaatt agaggtattt tctacgacta ttccatcttt ggcatttgag
     1081 cttgtgttta tttgattgaa tattttcagc gacaagatac cgctaagaat agtcaatata
     1141 gaaacaagtg taattaatac tgataatttc ctttttttgt tcattttttc ttaacttcct
     1201 ttttgttttg attagttaga tattccttat ctaacaagcc tttttaggct attaaatgaa
     1261 gataaacccc aaaaaaatat tgcaattatg acagtgcaat ggtactatta ttatacaata
     1321 aattttgcga aaggggtagg tctaaatcgc aaacaaaaag aaattgaacc tagacaaata
     1381 caacttgtac accgcaaggg aagcaagttt gattttgggt aaaggtagaa attatgttac
     1441 aaacataatc aactattatc caaatcgttt gcctaaaggt tcgttcaaga aagtgggtga
     1501 caaaacctat attataacgg aagaaggaat agaagcattg cgatcacaaa cagaaaataa
     1561 aaacaaaaag aaggaatgaa tatgaagaaa aattcgcttg gtatggtttt atttgcatca
     1621 gctgtacttg cggggttatc agtaccagta acctcacatg ctgatgaatg gactgcaaac
     1681 acaccggatc aaatccaaat tacaacagga caatcaagtt atgcgttcaa aaaaggagat
     1741 actatttggt caactgtaga gaagcttaaa agcttaggtt acaagactac tgttgctgag
     1801 tttgccgaat tgaacaatgt tgatttggca aaaggagaac aacggactat cccaattgga
     1861 cgaactttca acatcagtaa agatacatta acagttaagg aagcagatgg cgaagtagtt
     1921 agtcaaactg ttcaaaaaga taaacctgat cctgttgaaa atacggagca agagaacaaa
     1981 aaatccgaga ctgcaaaaga atcggaacca acagcaaaca cttctgataa gacagaaaca
     2041 agtgcttcaa gtgttgatgg agcaagttca tctaatacaa ataattctac aagtgagaaa
     2101 gaagcatcac agggaacaag tgaaagtagc actacggaaa ctgatactgg aacatcaaca
     2161 aataactcac aatcaacatc aaatgatgca aatgaatcaa ccagtacatc ttctagtgat
     2221 caatcacaaa caggttcgag tgatgaaact gacagttcta cttcaccaag taaacccgaa
     2281 caaccaacta atgttgtgct aggcgttcca tttattagtc aaggtaacac aatgctttgc
     2341 gaaggaacat ctttgctaga agcgttacat tataaaggtg ttgcaacaaa tcaagattta
     2401 atgacttttg ttaaatcaat gccactatct cctgataata atccaaataa tggtttttct
     2461 ggtgaatgga gacacaatgt ggatggaact tatcaaggta tgaatccggc tcctgtagtt
     2521 tcatggggtg ttgctaatgg cggaaatgtt gtagatattt ctggttcatc ggtacaagga
     2581 ttaaaagatg agattgtaaa tggaaatcct gtagtagcgt gggtaactta tgcctttgaa
     2641 cctgctcaac ataaatcaat gttttggggc gatgctattt ggaatcgaca tgtagtagcc
     2701 gttgatggat ttaaagacgg tttttaccat gttgtcgatc ctgtgtttgg ggcaagttgg
     2761 attaatgcag gatcatttga aacagcatat aacgtatctc aattagcggt ttcggtacgt
     2821 taataaaaga gattgtttat atactaagta gttaagtata gtctttattt gtccttgctt
     2881 aactacttag aaaacttgtt gctcatggtg ggtagaacta aaaggggtga tgctatgaat
     2941 actagagcgt tgaaacagct tgctttcaac gaaaaaagtg attaataggt gtggtgattt
     3001 taacttagga atgggcgcga aggtcattac cacaccgaca tatcgtggct cattttcttc
     3061 gcctaactgg tataaagaag gagtaacgag agatgaagca tgaaaaaacg ataattttaa
     3121 aatcaaaaat aatttattgt tttgcgactt tagtaatggt gttatctacg cttataatgc
     3181 ctttttcagc aataagagct gaagcagatg tcacaaaacc gacaagtgca aaatttacag
     3241 aaactggcgt aaaaacagac ggttatatta gggttaatat gccgaatcat ccaaatgaat
     3301 ggatgatttc aagtcaattt aaagattctc atggaaatat tggatattgt atggattcag
     3361 aattgccatc cccaacagga agtggagccg gaagcttgaa gtacaaggga gctggcagtg
     3421 atgagttcta tcgtatgttt aaaggtggtt ttccatcaaa aacagcaaag gaactgggtg
     3481 caggaaatga tacagaagca tggtatgcaa cacaattagt ttcatgggtt ttagccggaa
     3541 actttaaagt ttcacagatt gtatggtcac atccaaacca taccgccgca gaaacagctc
     3601 gtgtaaaaaa agcatttgaa aaaatttatg actatgctaa gaatggaaaa gatacgccca
     3661 acactgagtt ttctatcaca gcttctaaga cagctgacga aggaaaatat cacactttca
     3721 catataaaac agcatcaaat aagactggta atgcgaagct cacttttaca tcagcaaaac
     3781 cagctggtat gaagatttat gatgcagatg gtaaagaaat tactaacaac actgtgaaat
     3841 taaattcaag ttttactatt aaagtacctg tgactacacc aagtggtact ttgtcattta
     3901 aaggtactgc taatgttagc acaaccaatc cttttacttt tgatggacga ggtgtgtatc
     3961 aagatgccgt agttatgatc actacttcag aaacaaagga ctctaaatct ttgagtgcta
     4021 aatggacaag agctgttggt caggtaacag ttaagaaagt tgatcaagat ggaaatgctt
     4081 taaaaggagc ggagtttact ctaactgaca ataacaatgc taaatctgtt aagacaaccg
     4141 gagatgatgg gttagctacg tttgatttgc aacaaggctt aacatacagc ttagaagaaa
     4201 caaaagttcc tgaaggttat caaggttcat ttaaaaagga tggtattact gttaataccg
     4261 atggacaaga atttagttac actgctacga acacaattaa aaaaggtaaa gtaaaagtta
     4321 aaaaggttga ccaagatgga aaaccattag ctaatgctga atttaccttg acggatgatt
     4381 catcccaaac acaagcagaa aaaacagatg aaaacggact tgcaagcttc gatattgtag
     4441 caaacaagac ctacagcctt gaagagaccg gagtaccaac tggttataca ggcgtattca
     4501 agcaagaagg aattaccgtt aaagatgatg gacaaacttt tgaatataca gctacaaaca
     4561 ctttgaaaaa aggtttagta aaagttcata aagtcgatca agatggaaaa cctttggcaa
     4621 atgcagagtt cactttaacc gatgaaaaag gtgcaacaca agtagaaaaa acagatgaaa
     4681 atggacttgc aagcttcaat attgtagcaa acaagacgta tagcctagaa gaaactggag
     4741 taccagtagg ttacacaggt gcatttaagc aagagggtat caccgttaaa gacgatgggc
     4801 aaacttttga atatacagct acaaataatt tgaaaaaagg tgtagtaaaa gttcataaag
     4861 tcgatcaaga tggaaaacct ttagcagatg cagaattcac cttaacggat gaaaaaggtg
     4921 taaaagaagt taaaaagacg gatgcaactg gtctggctac gttcaacatt attgcgaaca
     4981 aaacttacaa cttagaagaa acaaaagtac caacaggtta cacaggcgca ttcaaacaag
     5041 aaggaattac cgtcaaagat gatggacaag tctttgaata tactgctaaa aacactttga
     5101 aaaaaggtgt gatcaaagtt cataaagtgg atgatcaagg gaaagcatta gcaaacgcag
     5161 aattcacttt gacagacgac aaaggtgtaa aagttgtaca aaaaacggac agcaatggct
     5221 tggcaacatt taatattgtt gccaacaaaa cgtacaaaat cgaagaaaca caaaacccta
     5281 atggttatac agggaaatgg tctaaagaag gcatcaccgt tgctgacgat gaacaaacct
     5341 ttgaatatac agtaattaat caccttgtac ctgaaacagt aagaacaggc ggaatcggta
     5401 catcagcctt ccttcttgta tttctaattg caggattgag tatggtgcta ctgtattatc
     5461 gagaacagca caaagcaaaa taaaaaagtg ttaatggaaa agaagtaaat atgaaaaaag
     5521 aaggctgacg tatgaaatca aaaaaaattt tttcaaattt aatgctattt ggtactttgc
     5581 taggagtggg attgggcgca agctcaatct cttctagcct tggactaggg gggcaagtag
     5641 catatgcaca acaaaactat aattatcaag gaactggaac gattcatatc cataaagtaa
     5701 caaatactaa tggggatggg aaccctgtct cagatattga taacaaaggg aaagaaatag
     5761 ctttaccaaa tggtgtaaaa cctcttgaag gcgttgagtt ctcttatgtg aaacttacta
     5821 aagatgaagc taaatccttg ggatggacgg atagtgatac tacaaaaggt caacatattg
     5881 atgatgctaa agcacgatta ttactaaaca aaaaatacag tgaagtaagt agaatgaaga
     5941 ctgattcaca agggaatata gacataccta tacaagtaaa tacagacgat ttaacagata
     6001 acttgtattt gattgttgaa acaggaacag tcaaaggtgt agttgaagaa gcgtatccta
     6061 tgattgttaa cgtacctgaa ctaaacggat cagatgatca atactttaat tatgatgtac
     6121 atgtctatcc taaaaactat ttccaattaa actcagcaga gcttgataaa aaagacgaag
     6181 atggaaaggc tcttgccgga actgtttttg aattgcgaaa agatgatgga tctgatcagg
     6241 ttgttggaac ttataaaaca gacagcacag gtaaattaac tgtagatggt ttggaattag
     6301 gtgactatta tttcctagaa accgacttgg gaacagatgc taataaaaca acctatttac
     6361 tagatggtaa aaaattcaaa tttagcgtta aacgtgggga gactgctaaa gtagttcttg
     6421 atgttaccaa ctacaaacga cctgtaatta ctaaggtaat tgataaagaa accgttgata
     6481 ctggcgaaga tgttcaatat acattgaaac catcaattcc tggtaatatt ggtaattacg
     6541 aaaaatatgt tgtcaaagat gttctaggtg cagagttaac ttatgattca tcagaagtga
     6601 tcgaaggaat gactttagat actgattaca gtatcgaacg tgacggacaa acgacaaact
     6661 atgtctttac tgaatcaggt atggataagc ttcagaaaca atttgatgca ggggatcgct
     6721 tctttacaat tttgatgaac gctcatacta atagtaaagc taaaatggat acaccactta
     6781 agaatgatgt ttcaacgatc tacaacaatg gatatgaacc cgaagacgaa acaccagttg
     6841 atcacgctta tacaatcatt ggtgatcacc aattcattaa aacagatgga gatacaagca
     6901 aagcattgag tggagcaaaa tttgtagtga agaacaaaat tgatccgaat aaagttactt
     6961 acctaaaaca agatccaaca acgaaagctg ttacttggat tgcagataaa tctaaagcta
     7021 ctgaattcgt ttccggatca gacggtaagt tccaaattga aggcttgcaa tatacctatg
     7081 atgtagattt tgataaacaa ggtaatccaa caatcacaga taaaaaaatc aatgattatg
     7141 ctcttgaaga agttgttgct ccttcaggat atacaccaac tagaaatgat gttccatttg
     7201 tggtatcgaa tgatgatgct tctagcgaag taacagacat caagaacctt aaaacacctg
     7261 aaacagttcg tacaggtgga attggaacag tagtatttct agtggttgca ggtgtggcag
     7321 gaacagggct atatgttgtc tacaaacgag aacaacacaa agcttaatga aataaaattc
     7381 cctagagtgt ttttggctct agggtttctt tttaaaaagt gtgtgaaaag taggtatgag
     7441 catgaaaaag aagaatatca agttgatcat cttaaaaatt gggatgttgc tatgtttagg
     7501 agtaggtcta tatcctgtaa ttactcaggc ttatacctac taccaaactc aacaaacact
     7561 aaactcctat aaggaaaaat atgatgaagc ttctgaaaag gagaatgaag aagtcttcaa
     7621 gagatattca gaagttaata ggaaacgaaa agacacagaa caaacctcag tcacgattcc
     7681 tgaagataat aacaatattc cgtcaaatgg aaatcttcag aaggataaat taggtgaatt
     7741 agtgggtgaa attcttatac caaagattaa tgtggatttg ccaatttatg atggtgtaaa
     7801 cgattttcaa ctatcagcag gagcaggaat attaaccgga actgattatc caactggtgg
     7861 gattggaaac cattctgtta tatcagctca ttcgggcatt cctaataaaa aatattttac
     7921 agatttagag ttgctcaaac ttggtgataa attttttatt gatatttcag ggaaaaagct
     7981 tgcttatcaa gtagatcgga taaaaataat cctaccaacc gaatttgagg atttgcttcc
     8041 tgtggcagga aaagattatg taacacttat gacatgtaca cctgttggag ataattctca
     8101 taggttactc gttagagggg ttagcatacc ttaccaaaaa agtattgttg agaaagaaaa
     8161 gaagaaagaa gatttaaagg taaaggcgca gatgaactgg tatcctatcg tagttgtagg
     8221 ggttttatta ataatagctg tgatagtcat tattaaaaaa atagtagcaa agagacgagg
     8281 aatgaaaaat gaagaataaa atactacttt ttttgactct tatttgcact ttggttttaa
     8341 caatttatcc tgcaaatcag ctctatcatc aattcaaaac aaaagagaat gtggcggaaa
     8401 tccaagagaa aattgagaaa aaacaaactc cttcaaaaga ggaagtggca aaaaaagtcg
     8461 aacaaataaa atcgaacagc ggtaaccaag gattagacaa tactcagaaa acagacggcg
     8521 gatcatttga tattggtgaa gctgaaggaa aggtcaatcc ttacacagga gatgtttttt
     8581 caaaaaatca aacgaaagat tttgaaccat ctacctattc aggggcggtt ggttatgtct
     8641 atattccaaa aataaatgtg ctagagcttt tgtttctagg cgcttcacag gaacatttat
     8701 atagaggtgt ggcacagatg agtgctacta gtctaccttt atcaggtaaa ggtcatcaaa
     8761 cagtaatagc aggtcatcgt ggcggttata cgtcaccaca atttctttat gttgatcaat
     8821 tagtagccgg agaccacatt tatatagatt atcttggtga aaaagcagtt tatacggttt
     8881 tccgaacaga cgttattaga aattacggtc agaattcaac actgactgat attcctgatt
     8941 tagatatgtt aacactttat acttgtcatc catatcctca gaactacgat cgtttattag
     9001 ttagaagtct aagagatgaa aaagcaaaat acatcacgcc acaagtaaca gttggcaata
     9061 ccgcatacgc tgatgccatt gatagcgagt acgtttcaga taagacagaa actgttacat
     9121 cttctaatga tttatttgag gatgttgatc gtgccaacaa ggaattagat ggaaataatg
     9181 atcttaatat tccggaacaa ggtagcgtag agatgcaaga cagaacattt gctgaaaagg
     9241 caagttcaat attcacagga attaccaata aacatcagaa aatgctttt
//
LOCUS       DACDIE010000086         9280 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.086, whole genome shotgun sequence.
ACCESSION   DACDIE010000086 DACDIE010000000
VERSION     DACDIE010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9280
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.086"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            19..1278
                     /locus_tag="IV250_002475"
     CDS             19..1278
                     /locus_tag="IV250_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP8769720.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            1288..2160
                     /locus_tag="IV250_002476"
     CDS             1288..2160
                     /locus_tag="IV250_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8769721.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            2172..3002
                     /locus_tag="IV250_002477"
     CDS             2172..3002
                     /locus_tag="IV250_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8769722.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            3042..5225
                     /locus_tag="IV250_002478"
     CDS             3042..5225
                     /locus_tag="IV250_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP8769723.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            5286..6506
                     /locus_tag="IV250_002479"
     CDS             5286..6506
                     /locus_tag="IV250_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HAP8769724.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            6503..7963
                     /gene="gtfA"
                     /locus_tag="IV250_002480"
     CDS             6503..7963
                     /gene="gtfA"
                     /locus_tag="IV250_002480"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HAP8769725.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            8040..9146
                     /locus_tag="IV250_002481"
                     /pseudo
     CDS             8040..9146
                     /locus_tag="IV250_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT         3127 a         1454 c         1827 g         2872 t
ORIGIN      
        1 tataaggagg agcataaaat gaagtggtct aaaaaaatta ttttagcttg tatagcacta
       61 ggagcaacga tctctttgac tgcttgtgga aaaaatcagg aagatgcaag cggtaaggta
      121 gaaatagagt tttttaatca aaaaaaagaa atgactcaaa cgattcagga gattgctaaa
      181 gattttgaag caaaaaaccc tgatattcat gtaaaagttg tggatgtacc gaatgctggt
      241 gaagtaatta aaacacgtat gcttgcaggc gatgtaccag atgtcattaa tctttatcct
      301 caaagtatag aattgaaaga atgggcgaaa gctggttatt tagaggactt aaccgaagaa
      361 ccgtatttag agaatatcaa aaatggatat gcacaacgtt ttgctattga ggatcgtgta
      421 tatagcatac cattaaccgc taatgtctat ggtttttact ataacaaaac tgcttttgag
      481 gaaatgggga ttcaagcacc agaaacttgg gcagaatttg aaaaaatagt agctgagatt
      541 aaagatcaaa acaaagtacc gtttgcgatt gcaggagcag aaggctggac tttaaatggt
      601 tatcaccaac tagctatagc gactgctgct ggtggtgaag aggaagcgaa caatgtttgg
      661 cgtttctcag aagttaatgg aattaatgct gaaagcaaag aaatgcaaaa agattttaat
      721 cgattagatt tgttaagaga accaaaagct ttgcaaaaca actggcaagg tgccggttat
      781 aacgatacag tagttacttt tacaaaaggt gatgcgttga ttatgccgaa tggttcatgg
      841 gcaatgccga tgattcattc acaaaatcca gattttgaag taggaacttt tccttttcca
      901 gcagatgaag caggtcaaag tctaacgatt ggcgcaggag acctagcctt atcagtttct
      961 gcaacttctc aacataaaga agaagccaaa cgttttgtag aatatatgac aacacctgaa
     1021 gcgatgcaga aatattatga tgtagatggt gcaccgtgtg ctgttgaggg cgttatcgag
     1081 aatactcagg attcaccatt aagtggttta actgaacttg cttttactga tcgtcattta
     1141 gtttggttag ccaaagattg gaatagtgaa aatgattttt ataccttaac aacaaattat
     1201 ttacacaatg gcaatcaaaa agccatgatt aacgcattaa atgctttctt taatccaatg
     1261 aaagctgacg gaaggtagga aaataaaatg aaaataaaag aaattttaaa tcgttactgg
     1321 gcaatcattt ttgtagcaat tccaattatt ttgcagatga tttttttcta ctttccaatg
     1381 gttcaaggtg ctttttacag tttaactaat tggactggtc tgacttataa ttttgatttt
     1441 gtgggtttga acaattataa gattcttttt tctgatccga agtttatcaa atcgattggc
     1501 ttcactttaa ttgtgacgct ttgcatgatt gctggacaaa ttggttttgg tattttgatt
     1561 gcgcgggcgt tgaacgcaaa tattaaaggg aaaacattct ttagagcatg gttctttttt
     1621 ccagcggtct tatcgggcgt ggttgtttct ttagtattta aacaaatttt taactatggt
     1681 ttaccaaaaa ttggtagtat gttaggcatt gaatttttac aaaccagtct tttaggaaca
     1741 acatggggag cgatttttgc agtaattttt gttttacttt ggcaaggaat tgccatgcca
     1801 gtcatcattt tcttagcggg ttttcaaagt attccacaag aaattattga agctgctaga
     1861 atggatgggg cgaatgataa gcaaattttt tggaatattg aaatcaccta cttattacca
     1921 agtatttcaa tggtatttat tatggcatta aaaggtggct taacagcatt tgatcaaatt
     1981 tttgctttaa ctggtggtgg accaagcgat gccacaacat ctttggggtt acttgtttat
     2041 aattatgctt ttaaaaacaa tcagtttggt tatgcgaatg ctattgcttt agtcttattt
     2101 atcttaattg ggattgtttc actggcacaa ttaaaattat cgaaaaaatt tgaaatctag
     2161 gaggtttccc aatgaaaaca tcaaaagaaa aaaatatttc taaatacttg cttttattat
     2221 tagggggctt catgatttta gttcccttag tttcaacgct attcggttca tttaaaacaa
     2281 cacccgatat tatgaataat ttctttcgtt ttcctaatcc agcgactttg gaaaatttcc
     2341 gaagattgtt agcggatgga attggtcatt acttttggaa ttcggcaatc atcacaatca
     2401 ttgcagtagt tgctgtgaca gcaattattc ctatggctgc ttattcacta gcaagaaaaa
     2461 tgactcgtgg gaaagcatac ggattgatgt atttattttt aattttagga atttttgttc
     2521 ctttccaagt aattatgatt ccgattaccg ttatgatgag taaaattggt ttagcaaata
     2581 tgtggggatt aatcttactc tatctcactt atgccgtacc acagacacta tttttatatg
     2641 ttggttttat taaactaaat gtgcctatca gtctggatga ggctgctgaa attgatgggg
     2701 ctagtcactt tacaacttat ttcaaaattg ttttcccaat gttaaaacca atgcatgcga
     2761 cgactattat tcttaatgct ctatggtttt ggaatgattt catgctacca ttattaattt
     2821 taaatcgtga ttctaatatg tggacactac cattgtttca atataattat acgggacaat
     2881 attttaacga ctatggtcca agttttgctt cctatacagt aggaattgtc acgattacac
     2941 tagtttatct tgctttccaa agaagtatta tagctggaat gagcaatgga gcagtgaaat
     3001 aaccaataaa ttattctcat tataaaggag ttcataaact tatgattata tttgacaatc
     3061 acacacaaac atttcattta caaaataaaa aaattagcta tctattagga atcgaagaaa
     3121 ataattattt aacgcatctt tattttggaa aaaagattac gcactattct ggtgggtatc
     3181 gctacccccg aacagatcgt tccttctcac ccaacaatgc aaatgcgcaa gatcgtttat
     3241 tatctttaga cacattacca ttagaatttc caggatatgg acatggtgat tttagagaac
     3301 cggcgcataa tattaaatta ggaaacggta gtcgtgtaaa tgattttcgt tatgatagct
     3361 acgaaattca aaaaggaaag cccagcttag cgggattgcc agccttatat acggaagatg
     3421 atgatgaagc tgaaacgctg attattcaat tgaaagacgc gattagtgga ttgactttac
     3481 agttgttcta cacaatttat caaaatcaca gtgtaattat tcgttctacg caattagtaa
     3541 ataatagtga tgaaacagtg gagattaacc gcttagctag tcaatcaatt gattttccaa
     3601 atcgtccact cgagttaatt catttaaatg gtacctgggc aagagaacgt caaatgacta
     3661 gagaaaaaat tcatacaggg ataaaagtat tagatagtaa acgcggatca agcagccatc
     3721 aacaaaatcc gtttgttgct ttagtagatg ccaaaacaga tgaatttcaa ggagaggcat
     3781 atggatttaa tttagtttat agcggtaatc acgaaacggt gattcaaaaa gatccatttg
     3841 accaaacacg ggtcattatg ggaattaatt catttaattt tggttggcaa ctaaaaactg
     3901 gcgactcatt tcaaacgcca gaagtcgtga tggtttatag taatgaaggt ctgaatacgt
     3961 tatcacagac gtatcatgaa ttgtataatc accgtttaat tcgtggaaac tatcgtttaa
     4021 aagaacgtcc cacgttaatc aacaactggg aagcaacgta ttttgatttt aacgaagaaa
     4081 aaatcatgaa tattgtcgat gatgcgaaga acttgggcat tgaaatgttt gttttagatg
     4141 atggttggtt tggcggtcgt gaaaatgact tcacttcatt aggtgattgg gttgaaacag
     4201 aaggcaaact tgcttgtggt ttggaaaatt tagccaagca agtgcatgat aacgggatga
     4261 aatttgggat ttggtttgaa ccggaaatga tttctgaaga tagtgaacta tttcgagcac
     4321 atccagattg ggcattgggt attccaggtc gtgaccgctc aattagtcga aatcaatatg
     4381 ttcttgattt ttcaagacaa gatgttcgtg ataatatcta tcaacaaatg acggcgattt
     4441 ttgatcgtgt accgattgat tatgtgaagt gggatatgaa tcgaaatatg acagaagtct
     4501 attctgtctt gttggactca acgatgcaag gtgaagtggc tcatcgttat atgttagggt
     4561 tatatgaatt tttagaaaag ttaacaacga attatccaac tattttattt gaaagttgtt
     4621 cgggtggagg cggtcgtttt gatgcaggtt tcttgtatta catgccacaa acttggacga
     4681 gcgacaatac cgatgccgtg gctcgtctga aaattcaata tggaaccagc ttagtgtatc
     4741 caatttcaag tatgggtgcg catgtttcag cagtcccaaa tcatcaaaca ggaagagaaa
     4801 cgagtttaac tattcgtggc aatgctgcga tggctggtgt ttttggttat gagctagatc
     4861 taacgcaatt aacttctgaa gaaaaagcag aaattaaaga acaagttgcc ttttataaaa
     4921 agcatcgcca acttttgcaa tatggaactt tttatcgctt ggaaagtccg tttgaaggca
     4981 atgatactgc atggatgttt gtctcaaaag accaaacaga agccatggtc ttttatttcc
     5041 gagtattagc agaagctgcg catccattag tcacattgaa attagcagga ttagcagaag
     5101 aagcaactta tcaaatggaa gatgtaacat ttgctggcga tgaattgatg aatctcggtt
     5161 tttatattga ctcagaacta catggtgatt atgcgacaca acgcttttat ttgaaacaga
     5221 tatagtagct tgaatttaca atataagtgc aacaccaaaa gagactcatt ccaaacgaag
     5281 aaaatatgtt ttatttacac gcatttacta aaaagcagcc tgatttgaat tgggaaaatg
     5341 aagaagtacg tgaagaaatc tatgcaatga ttaactattg gttagataaa ggactaggcg
     5401 gatttagaat cgatgcgata ttaaacatca aaaaaaaact agagtatggc atctttgaat
     5461 ctgatggaga agatgggtta gccttcattg ggccctggat tcttaatcag tcgggcattg
     5521 aagtgtggtt aaaagaaatg agagagcgga catttaaacc acataatagt atgacggtag
     5581 cagaagccga tgttcctaat gaacgtttag atgaatatat tggtgaagat ggctattatt
     5641 cgatggtttt tgattttagt tatacggata ttgatgtacc tgaaactggt gaatggttta
     5701 aagatagcca atggacatgg acggatatgc gtaccaatat ttttacgaat caattagtta
     5761 cgcaagataa aggttggggc gccttatatt tagaaaacca cgatcaacca cgttcaatta
     5821 ataaatatat tccagaagaa tggatcaatg attatagtaa aaaaatgttg gcgacgcttt
     5881 ttatgttact aagaggtacc ccttttattt atcaaggaca agaactagga atgaccaaca
     5941 tagaaatggc atcattagaa gattttgatg atgtagctac gcattctcag tataaacggg
     6001 cattagaata tggattggca ccagagcaag cattgaaagc tgtcaataaa cgaagtcggg
     6061 ataattctcg cacacctatg caatggaata cgcaaaaaaa tgccagattc tcaacttctg
     6121 ataatgtatg gttaaaagta aatcctaatt atccagaact caatgcagaa gctcagttaa
     6181 ccaatcaaca gtcggtattc aattattatc atcaattaat taaattaaga aaaaatagtg
     6241 tatacaaaga cgtgctaatc tacggacaat ttctaccagt gaaagataaa aatgaacata
     6301 ttttgcttta tgaacgccaa ttagaagata aaatcgtatt agttatctta aacgttacag
     6361 caacagaaca agaatattat gtagatgaac agtaccaaca agtattaatc aataattatg
     6421 aagatgtttt attagatcaa agtcgaaaat tgcgtttacg accgtttgaa agtattgtat
     6481 tagctaatta tggaggacaa ttatgaaaat taaaaacgaa gcaatgctca tcacctatgc
     6541 agatagttta ggaaaaaaca tgggcgaatt aaaacaagtc ttagacaaac atttacaagg
     6601 tgttattggt ggtgtacatc tgttgccttt ttttccatcg acaggtgatc gaggctttgc
     6661 gccgagtgat tatacgacag ttgatccagc tcttggaaca tgggaagatg tggaagcatt
     6721 aggtgaagat tattatttga tgtttgattt tatgattaat cacatttctc gtgaatcaaa
     6781 atttttccaa gatttcaaaa aaaaccatga gcagtcacca tacaaagaaa tgtttatccg
     6841 tattcatgaa tttttcccag aaaatcgacc aacacaagaa gatattgatt taatctacaa
     6901 gcgtaaagac aaagcgccgt ttcaaacggt agaatttgcg gatggtacga cagaagaggt
     6961 ttggaatacc tttggggaag aacaaattga tttagatgtg accaaagaag ttgtgaaaga
     7021 atttattcga gaaaccatca aagatatggc gaaccatggc tgttctttaa ttcgattaga
     7081 tgcgtttgct tatgcgatta aaaaattaga taccaatgat ttcttcgtgg aaccagatat
     7141 ttgggaactt ttagacgaag tgcgtcaaga agcagcgaaa tatgacgtcg aattattacc
     7201 agaaatccat gagcattatt ccattcaaat gaaaattgcc aatcatgatt attatatcta
     7261 tgacttcgcc ttgccaatgg tcactctgta ttcactttat agtggaaaat cagaacgttt
     7321 agccaattgg ttgaaaatga gtccgatgaa gcagtttaca acattggata cgcatgatgg
     7381 aattggtgtt gtggacgctc gtgatttatt aacagatgaa gaattggatt acacctctga
     7441 ggaattgtat aaagtcgggg cgaatgtgaa gaaggtctat tcaagtgcca attacaataa
     7501 tttagatatt tatcaaatta acagcaccta ttatagtgca ttaggcgata atgaccgtag
     7561 ctatctgtta gcacgtgcga ttcaagtatt tgcgccaggg attccacaaa tttattacgt
     7621 tgggttatta gcaggaaaaa atgatattga actgttggaa gcaacgaaag aagggcgtaa
     7681 tattaatcgt cattactatg atttagccga aattgaagaa gaagtgcaac ggccagtgat
     7741 tcaacaatta tttgcacttt taaaatttag aaatgaatct gcggcgtttg atttggatgg
     7801 aacgattgaa gtagcaacgc catcagaatc agaaatagtg attacacgta aaaataaaaa
     7861 cggtagtgat agtgcaacat taacggctaa cttggatacg aaagaattta tcattgagtc
     7921 taaagatata aaaattttca ctagtgttgc attaaaattt taacctattc catagaacaa
     7981 aaaatccttt atactagcac ttggatgact tatccaagac caataaaaag gactcataca
     8041 tggataagta taaaacaaat acagcattta ataaatggtt ttctgccata aaattaggaa
     8101 aactttctgt caactcccaa aaatgtattt ttgattacaa ttcgtatcgt aaaaaactgt
     8161 cttttgaagc tgtgctcaag ctctttctct acgcagttaa tgaagaaaaa gaaagtttgc
     8221 gtgatttgag tacagctctt atcgaccgat cgctccaact agaaaccgat gtgtcatcga
     8281 ttagtcatac ccaattgtct cgagcattta atgcacttga acctaaagtg ttagaagaaa
     8341 tttttcaaca actgttagaa aaagttcaac atcaaatcaa accaaccaag cgaaatcatc
     8401 tgtatctgat tgattccagt acgttttcgc ttagtttaaa acgtcataag tgggcgactt
     8461 tccgtcaaac aaaatcaggt gtcaagttac acttaaacta ttgttatatg gacgatacta
     8521 cgatgtatcc aactgatttt accttaacaa atgcccatga acatgatctc aatcaactac
     8581 cagtattggt gaatcaacca gaggcaactt acgtgtttga tcgtgggtat taggactttg
     8641 aaaagatgga tcagatgcat tgggatggct atttcttcgt cactcggatt aaaaaaaaca
     8701 ccaaagttca cgtgatagat actctggaaa catcgccaga aacagaaatt ctacgagacg
     8761 agctggttcg tttaggatct aaaacctatc taaccgccaa tttccgacta gtcactgttc
     8821 aagataagaa cggacgtgtg tttcaattta tcaccaatcg aatggacgtc tcttcaaaag
     8881 aaatctctga tatgtatcac gctcgttggc aaatcgaatt gtttttcaag cacatcaaac
     8941 aacacatgac cattaaaacc ttcttttctc aaagtgaaaa aggcgtgcag aatcaattga
     9001 ttttaacgat gatttccgct ctcctcacgt ttctcatcaa attggagacg aaaacggaaa
     9061 aatcagtgtt ccaaatcaaa cgattctttc gttatttact gtttcagccc tttgaatgtt
     9121 gtctagaaaa attaattccc acatgagaaa aaagaaattt ggctcgttac ttttggtttt
     9181 cagttgaata acagataaaa aattttctgg aaaaagtcat cctctgcgtc agcacagttt
     9241 tttttgactt attttcagag gaaatagttt tcagaatatt
//
LOCUS       DACDIE010000087         8916 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.088, whole genome shotgun sequence.
ACCESSION   DACDIE010000087 DACDIE010000000
VERSION     DACDIE010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8916
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.088"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            232..1230
                     /locus_tag="IV250_002482"
     CDS             232..1230
                     /locus_tag="IV250_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769726.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            1243..3261
                     /locus_tag="IV250_002483"
     CDS             1243..3261
                     /locus_tag="IV250_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAP8769727.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(3321..4037)
                     /locus_tag="IV250_002484"
     CDS             complement(3321..4037)
                     /locus_tag="IV250_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAP8769728.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(4264..4806)
                     /locus_tag="IV250_002485"
     CDS             complement(4264..4806)
                     /locus_tag="IV250_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP8769729.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(4811..5038)
                     /locus_tag="IV250_002486"
     CDS             complement(4811..5038)
                     /locus_tag="IV250_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopZ"
                     /protein_id="HAP8769730.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(5117..6997)
                     /locus_tag="IV250_002487"
     CDS             complement(5117..6997)
                     /locus_tag="IV250_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP8769731.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(7186..7800)
                     /locus_tag="IV250_002488"
     CDS             complement(7186..7800)
                     /locus_tag="IV250_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8769732.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            7942..8502
                     /locus_tag="IV250_002489"
     CDS             7942..8502
                     /locus_tag="IV250_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8769733.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
BASE COUNT         2697 a         1732 c         1441 g         3046 t
ORIGIN      
        1 gaactaacga catatccaaa aacttctcta acaaatgacc accctttcac aattttaaca
       61 atcattgtat cattttatgc aaagaaacta aaggtataat tttctaaaaa ttctaacaat
      121 acaaacaacc atttcttgct aattttgtac tttttctgtc tacacgttgt tttttgagat
      181 attctaagtt aggatgttat taatgggcaa cttatcagga aacgttttct tatgtttttg
      241 tccaattttt ttgtgaggtg tttattcttg aaaaaaataa agaaggagag ttatggcttc
      301 ctgacaatct ttgtcgcttg tataatgatg gttttacagc gtatattgat ttcagcagtc
      361 actattccta catatgcaac aaaccctggt cgattctaca tctatacatt tttacaaact
      421 tccgttgtta ttggtgcaaa tttgatccca ctttacatgg gttaccaata tcaaaaaatc
      481 gcccgtttaa aagttttaca ttatttaagt cgttttgcct ttatattcct aatagccaca
      541 ctagtttgca atatcttttt ttacgtacaa gctggcagct tgaatattcg tgattattgg
      601 cttatcctaa cacctatcag tcagaattac tttacttatg ctgtttcttg tactttgctt
      661 ttttgtagta ttccttatac agttggattt ttaaataaat tatcgaaaac aacaattaaa
      721 tatctactat tatttttaac aattggatta gttggctgct ctactctatt taataaagac
      781 ccgtttgcat tacagaatgg acagagtatt ttatggattt tttatttatt tttaatgggt
      841 tatggtttga aagaatggaa ctggaaagaa aaggtccgct ataagcttcc tcaagtattt
      901 atcagcttga ttgttttatt cggtttgatt attttgatga cacaaatttc actcatcatc
      961 agaggaaata cagatacagc gctgaggttt tctaaaccat attctctctt cgcaatgtat
     1021 tatactttct cttcgttttt atttttagaa atgctatctg ataaattagg tataaaagga
     1081 agaggaaatt ttcttgctgt atttttgatt gtcactcaag ttatatgtaa tttcccttta
     1141 gtttcttatc gcgtatctac atttatgaaa cgtgaatttc caaactcagg gaaggcatgg
     1201 ctgataaata tcgggcagtt ttttatttga tatttattag cagtgtccct cctagttatc
     1261 tttttatttt atatacaaaa aatccgtttc attcatcgtt tcatcgaata cttcgctttt
     1321 gattcgattg aacaattaac tcagcgttgg aaaaaaagaa ttcattggct ttttgttcat
     1381 aaaagttatt ttttagttgc agcatttttt tactgcttta ccttcgtgca gattttcgtt
     1441 ttagaaccaa aagaaggttg gaaagaaact atccaggttg ccttaaaaat atttactcag
     1501 cgacaagcac ccatgatcct gacaatcatc attattatgg gatttttctt cttactattg
     1561 ttgatttcca atcgtttttg gtacgccttg actgctacct tgatcattaa cttactgctc
     1621 actatttcga cagtaatcaa aatggaaatg cgggaagagc ctgtctttcc ttcagattta
     1681 aaaatgctga ccggattgtc agaactatta tcgatggtta gcccagtctt attaatcgtg
     1741 ggcggattga tccttctcct attattgatt actagtatta ttgtacaacg tagactacaa
     1801 aaacagtatt ccttgaagat tcattggaaa cgtcgattta tcagtatcgc tgtattatta
     1861 ggatgttttt cgggtgtctt tttcattaat cacaaaaatt cgccttcttt cttgctgttt
     1921 aatttattta aagtaaacaa gactttcttt aaccagcaag gcgccgtaaa agataacgga
     1981 ccagtcattc aattcctaaa caatatcgac attaaaatca tggaagaacc tgctggctat
     2041 tcgaaagaaa aaattaatgg aataatgaaa aagtacaaca caaaggcaga tgaaatcaac
     2101 caaacaagaa gtgactggtt aaacaatcag acgatcgttc tcaatttaag tgaaagcttc
     2161 agtgatcctg cacgtgtccc taatctgaca gtacctacag accctattcc ttacatcaaa
     2221 agcattataa cagaaggaac aggaggattg atgttatccg tcggttatgg tggcggaaca
     2281 gctaacatgg aatgggaagg actgacaggc ttgagtatca gtaacctgtc tgcttcgtta
     2341 gttacacctt atacccagct agtggagcgt cagaaaataa gtccaaatat taccgatcta
     2401 ttcgatgaaa aaatagccat ccatcctttt acagctgctc tttatcgcag aaaacaagtg
     2461 tttgataaat ttggttttga caggttctat tatttagaca gtccggataa attaacttat
     2521 acggataaaa tagaacaaag cccacgcata tcggatgatt cagcatacaa agaaaccttg
     2581 aaagctttac aaagcaatac ggaaacgtca caattcatcc aattatccac tatgcaaaac
     2641 catatgcctt atgatgatta ctatagtgaa ttgaactaca ctgctgaagg aactgctgtc
     2701 attcctaata gaagaaaaga attacaaact tatatgcaag gccttcacta taccgataca
     2761 gcagtaaaaa aattcatcca agaaatcgac aaaatcgata aaccgattac ctttgttttt
     2821 tatggtgacc atttacccgc aatctatagt gggaatgatt cgaaaaaata tggattagaa
     2881 cagcaccaga cagattattt catttataac aacaagtata gtcgtgaaca agcagctacg
     2941 ttaaataaag aagttgttgc tcctaataac tttggtgcta tggctcttga gcatgcgaat
     3001 atcaaagtaa ctccttacta tgcgttgttg acagaagtat ctaatcatct acctgcatta
     3061 acaattgatc ctacagaaag cttgtctaat cgttttaatg gaaaacaggt ctttgttaca
     3121 gaggatagtc aaattatcca agaaaaaaat ttaactgaga aacaaaaaga aattctagaa
     3181 gattatcgtc tgatacaata tgatcttgtt gcaggtaacc agtacagtgc aacatgggcg
     3241 acacaaaaaa taaaagacta acaaataaac tgagcaaggg gttgtgataa aagacatgtc
     3301 acaacccctt gtttatctca ttaatttgct tgtccataaa cgatttgatt aaagccattt
     3361 ttcaattcat tattcgctgt ctcgttgaag taaagataat tatcgggatt cccctgattt
     3421 tgttgtgtca ccaatacttt tggctgattt tgctgaaacc cgaatagata gacgtgcttt
     3481 ttcagatatg gttctgtctc tgcatcggaa tagacggctg tgatttgata gtctgcttga
     3541 cctgtcccac tttctgacca ttctgcagaa acaggcactc caccaatagc catcttccac
     3601 tccccgttca aaatagcttg agggacttta agaccatata agtccacact catttgattc
     3661 gtataacttt tgtattgttg tcccattgtt tggccccatt tggtcataaa tgcctctaat
     3721 tgtgcggatt tgtctgcatt ccacagtgtt tgttcaactt gttcgctgct ttctgaagag
     3781 gaagatgaag cctctacaga agaacttgaa gtagatgttt gagttgtttc agtcgttttg
     3841 gaagactctg acgaagtaga ggtatctgct gtttctgtac ttgctgcagt tgacttgctc
     3901 tctgtgctag ttgttgtagt agttgcaact gttgctttcg ctgtttcttt tgttgattct
     3961 tttttcgatt cattcccgtt ggtacaacca gctaacaaaa aagctagacc aattgttact
     4021 aaaatccttt ttttcaatta aaaaactcct ttcgattact cccacttctt tattctatca
     4081 aataagcaat gtgattttat tgatttttcg tattttctca tcaatctttc aaatcttcta
     4141 aaaactgaga agcacccgct gatgttgtct gtaatacatc agcgggtgct tcttttattt
     4201 taatcttcgt gattttccaa tttacctcac gctttatatt tgtatttgaa gaaacataga
     4261 cttttagtca tcatcgtctt cctcatccaa atcttgtcgg acagtttttc cttggagtct
     4321 ttgtacttgg tagatgattt ctttattgaa acttaaaagt tcaacaagca attgttgcaa
     4381 gccatatttt tcttcttttt cagctaattt gattgcccgt gtgataaaga cattttggaa
     4441 atcaaaatct tttgcaaagt tttccaacat tgttgaggct ggtaaatatt tgttttgacc
     4501 atgttcgtaa atttttccat aacgcatgac ttcttctaaa gtagacggaa cgatttcatt
     4561 ttcaatcact aactcatgag agattttatt gaaccatctc gcttcttgat cagcatattc
     4621 tgaaaaaagg gtacttaatt cttgcgaaga tgtacccaaa acatacaatt gtgcttggat
     4681 caatttcatt tcttctaatt taaaatttgc taatacatgc cccaccatgg cgccagcagt
     4741 tggtttatga tggtcgatat ctgcttgttt tgtttcttga acaaattttt cttccactat
     4801 attcattgat ttacgcctct tttacttttg ctgattggac ttcataacct aatttttcca
     4861 caactgcagc taattgttca gggtggactt catctggatc aaagtttgct ttgattttgc
     4921 tcgcattaaa caaaactttc gtattgctta ccccaggctg ttgagaaacc gcatgttcga
     4981 ttttttgcat acagcttgga caagctagtg tatctaattg cataataatt tttttcatcg
     5041 tagttcttcc ttcctttttc ttgatagtct tattctagat tacagcttcc ataaaaaaat
     5101 tgattcttat caagttttac gatttttctt ctttgctttc gtgtccaaaa tacaataaac
     5161 gcatcgcgtt aaagataacg atcaagatac tagcttcgtg aacgaacatc ccactagcca
     5221 tgtaaataaa cccggcaaat aagccgttga gtaaaaataa gacgacacca atggcaatca
     5281 ctacattttc acgggtattt aaaacagttt tcttagccaa gcggtaggca tgaaccaacg
     5341 attcaaaatt agattgcatc aagacaacgt cactcgtttc gatagcaaca tccgttcctg
     5401 atcccatagc aatcccgata tcagccgcag ccaaagaggg agaatcattg attccatctc
     5461 caataaaagc aacgcgcaat ccttgttctt ggtatttttt cacaaattga accttttcgt
     5521 ctggtaataa ttcagcatgg acttcatcaa tgcctaacat atcagctaca taatcagcag
     5581 tcatctgatt atcacctgtc agcatcacta actgttttgc tcctgcttgt ttcaacttag
     5641 ctaattgttc agcgacttct ggtcgaatca catctgacac acctatgatt tgagtgattt
     5701 ttttgtcgat tgctacaata atgaccgagc tgcctgtttg ttgtaattga tgtaaatctt
     5761 gtacttgctt tggtgtgaga gaaagtttgt gagcggcaat caatttggtg ttgcccgcac
     5821 aaactgtatg accatcaatc tcagctgtga ttccttgtcc cttgattgtc tgattatctt
     5881 tgactgctaa ttgttgaaaa tcaatgtctt tctcctgagc ataatggatc acggcgcgcc
     5941 ccagaggatg atcggataac gtttctaatc gagccactaa agctaagctg tccccttcat
     6001 cctctgtata gttcttgata gtggatacag cagtagttcc tttggtcaat gtaccagttt
     6061 tatcaaagac aaaagtatcg attttggaaa aagtatccat tgcttcaccg cctttgacta
     6121 agatgccccg tttcgctcca ttaccgattc ctgctacatt cgaaacaggc gcaccaatga
     6181 ctaaagcgcc aggacaacca agaaccaaaa cagtgattgc taaacgaaaa tcacgactaa
     6241 tcagaccaac aaccaacgca ataagtaaaa cggttggagt ataatactga gaaaatcgat
     6301 caatgaattt ttccgcatgg gatttggtat cttgtgcttc ttctactaat tcgataattt
     6361 tggcaaaggt cgtatcatct ccgaccttag ttgcttcaat cgtcacgtag ccatctgcta
     6421 aaatcgttcc agagaagact tcatctccac tgattttgac gacttgtcgt gattcaccag
     6481 tcactgcggc ttcatccgca taccctttcc cgtcaatgac tcgtccgtcc acaggaatag
     6541 ctccaccagt ttttactaag acgatatcgc cttcttccac gtcatcgatt tccacttctt
     6601 cagtcgttcc atcttcttgg acaacactag cagtagatgg ggccatctcg gtcaaagcct
     6661 tgattgactc ccgcgttttg gtcaatgttt tttgttccag atagctacca aacaaaaaca
     6721 agaatgtaac gatagctgac tccttgtatt ctccgatcat aaaggcacca agcacagcaa
     6781 tagtaacgag taaatcaatt gagataactt tgactttcac tgcctgatac gcatggatca
     6841 taataggtat gaccgcaata atagaagcta tggctagagc aatgttatag aacaagacaa
     6901 aaccagtaaa ttttccgata aaaccaaaaa taatcaacac agctgataag gcggtgatat
     6961 gatttcgatg ctcttggatc cacttttgta atttcatctt tctccctctt ttcctttcat
     7021 tcttacgaga ctaagtatag atacttatcc atcataaaaa attgaccact atcaagttta
     7081 gtcaaaaagg cggttttctt ttggtagaat acactacaaa aaatagttag caagaagact
     7141 cactaaaagt gatcttgcca actattgtcg tttctatcga aattttcatt gaatgatttt
     7201 cctgtacagc atacatcgga gatctatttc tttatctaag atttttatct tgctaatttt
     7261 ttcctttagt ctttcaaatc cacgtcgttc atagaattga tacgtacagc cactatctgt
     7321 aaaaaggaat atttcttttc ctttctctct tttttcaaat tctgttagca acttggtacc
     7381 gatgcctttt gttttcaaat caggatttgc agctaaaaaa atgatctctc catctggcga
     7441 atgcttcttt aaatatgctg caagcatttg ttcatttgct gtctcgtaag ctcctttact
     7501 ttctttagca aatatatttt gtaaaaaatc gaatatctta acatatattt gctgtccaaa
     7561 agaacgatgt ttcttttttt ctccttttat ctccgccagc aaaacacctg ccaactcgtc
     7621 ctctgcatac aaagcaagaa tttgagttgc tcgattcaac tccatataca aaaaatatct
     7681 tccgtaaaga ttcagcagaa gttcattttc catatatctc tcaaagttca ttccttcaac
     7741 agcaaaatag atagcttttt tgtaatcttt cttttctaag ggtttaaaag ttagtttcat
     7801 catttcgcct cctcaacttc aatgattgtg ttatacttac attataatag acacagtgtc
     7861 tactatctat aatcaatcat ctataagttt gtcgattaag cgacagcttt gctttgatat
     7921 gtcggataag gagataggta aatgaaaaag acagatagac gcgtcaaaaa aacagaaaaa
     7981 gctttggctg aaacactatc cacattatta gtaaataaaa agattcaagc aatcactata
     8041 cgcgaactaa cagaaacggc agatgtacat cgttcgactt tttatacaca ttataaggat
     8101 atctatgatc tatatgatca attagaaagc aacttttttc gggatctaaa tgaaattctc
     8161 tcttatgatc cagcccatag ctatgaggaa ctatacactc gcttgattga ctatgtttac
     8221 gcaaatactt ttttatatcg cttatttatc ctcaaccatg gtgatatttc gtttcagaag
     8281 aaagttaaac aagttattga agaaaactat ctgaacatat ggctatatga agacaagaaa
     8341 acgattatca cagaagaaat gagattcttt actacgtacc acgtccaagg ttgtctatcc
     8401 gttctcaatc ggtgggtaga aagcaattac aagtactcca aaaaagaatt attagaactc
     8461 ttacagaaac tgaatgatca catagagaaa atcatgcctt gaataaaaac atactaagct
     8521 tagttaagaa gaaccgtaac cttctcgtat aacaactgaa tcttatttag ccaaattgtt
     8581 tactgaagat tttttgagta aaatttctat aattcctact tattgaaaag tagtttgtag
     8641 tgctgcttct ccctctctaa gtgatatatc ttgttgtacc aatcaaattc acctcatcat
     8701 taataaatta cttttaggct aatttattac aacttatagg ttgaagctgt aaccaattta
     8761 tagtcatatt atatttttat catccagttt attgaattct tctcgtttac ctcaattcat
     8821 tttcaagggg aatcagctca tcatctaaca ttttacgcca tgctcgcttc atttcttgtg
     8881 ggtatttaaa tggtttcgct tctggatgtt tggctt
//
LOCUS       DACDIE010000088         8731 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.089, whole genome shotgun sequence.
ACCESSION   DACDIE010000088 DACDIE010000000
VERSION     DACDIE010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.089"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            2..74
                     /locus_tag="IV250_002490"
     tRNA            2..74
                     /locus_tag="IV250_002490"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35..37,aa:Asp,seq:gtc)
     gene            78..150
                     /locus_tag="IV250_002491"
     tRNA            78..150
                     /locus_tag="IV250_002491"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:111..113,aa:Phe,seq:gaa)
     gene            155..225
                     /locus_tag="IV250_002492"
     tRNA            155..225
                     /locus_tag="IV250_002492"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:187..189,aa:Gly,seq:tcc)
     gene            238..311
                     /locus_tag="IV250_002493"
     tRNA            238..311
                     /locus_tag="IV250_002493"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:272..274,aa:Ile,seq:gat)
     gene            323..394
                     /locus_tag="IV250_002494"
     tRNA            323..394
                     /locus_tag="IV250_002494"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:356..358,aa:Glu,seq:ttc)
     gene            660..1853
                     /locus_tag="IV250_002495"
     CDS             660..1853
                     /locus_tag="IV250_002495"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAP8769734.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1983..3455
                     /locus_tag="IV250_002496"
     CDS             1983..3455
                     /locus_tag="IV250_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8769735.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3538..4326
                     /locus_tag="IV250_002497"
     CDS             3538..4326
                     /locus_tag="IV250_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAP8769736.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(4393..5061)
                     /locus_tag="IV250_002498"
     CDS             complement(4393..5061)
                     /locus_tag="IV250_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP8769737.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            5176..6888
                     /locus_tag="IV250_002499"
     CDS             5176..6888
                     /locus_tag="IV250_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8769738.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGSPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6891..8663
                     /locus_tag="IV250_002500"
     CDS             6891..8663
                     /locus_tag="IV250_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8769739.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         2621 a         1487 c         1945 g         2678 t
ORIGIN      
        1 tggttccgtg gtgtaggggt taacatgcct gcctgtcacg caggagatcg cgggttcgat
       61 tcccgtcgga accgtttggc tcggtagctc agttggtaga gcaatggatt gaagctccat
      121 gtgtcggcag ttcgattctg tcccgcgcca ttatgcgggt gtagtttagt ggtaaaacca
      181 cagccttcca agctgttgtc gcgagttcga ttctcgtcac ccgcttttag tttttatggg
      241 cctatagctc agctggttag agcgcacgcc tgataagcgt gaggtcgatg gttcgagtcc
      301 atttaggccc atttatttta ttggcccgtt ggtcaagcgg ttaagacacc gccctttcac
      361 ggcggtaaca cgggttcgaa tcccgtacgg gtcatggaag atactttttg agtatctttt
      421 tctttttatc tgaaagaatg attttaaaaa gcttagcaga gttatcttta tggatgattg
      481 tgctaagctt ttttggacag ttctaatgta aaatattttt atatttttta agcaaaaaaa
      541 tgaaaaaata cgagcagaaa actgttttat ctgctatact tggttcgttg caagttcccg
      601 aaaggatccg caggacggag atgccttgta accgaattta actaggggga atcattataa
      661 tggtagaaag acacttattt acatcagaat ctgtttctga aggacatcca gataaaattg
      721 ctgaccaaat cagtgatgca atcttggatg caattttaaa acaagatcca acagcacgag
      781 tagcgtgtga aacgtctgta acaactggtc ttgttttagt atttggagaa atatctacga
      841 cggcatatgt agatattcaa aaagtcgtac gggaaacaat aaaagaaatt ggttatacac
      901 gtgcaaaatt cggatttgat ggagatacag cagctgtatt ggttgcgatt gatgaacaat
      961 ctcctgatat tgctcaagga gttgatgaag cacttgagat ccgggatgaa gataaaaaag
     1021 acgtattaga tgagattggt gctggtgacc aaggtttaat gtttgggttt gctgtagatg
     1081 aaacaccaga attgatgcct ttaccaatcg ctttgagtca tcgtctggta cgacgcttgg
     1141 cagatttgcg taaatcaaat gaattaacgt atttacgtcc agatgcaaaa tctcaagtaa
     1201 cggttgaata tgatgatcaa ggacaaccgg aacgcgtaga tacaatcgtt atttcaacac
     1261 agcatgatga tgcagtagat aatgaaacaa ttcgtcatga tgtcatcgaa aaagtagtga
     1321 aggaagttat tccagctgaa ttattagatg atcaaacgaa atattatatc aatccgactg
     1381 gccgatttgt cattggtggt cctcaagggg atgccggatt aacaggaaga aaaatcattg
     1441 ttgatacgta tggcggttat gctcgtcatg gtggcggtgc tttttctggt aaagatgcga
     1501 caaaagttga ccgttctgcc agctatgctg cacgttatat tgctaaaaac attgttgcag
     1561 cagggcttgc tcgcaaagca gaagtacagc tagcttatgc gattggtgtt gctcaacctg
     1621 tttcgatctc aatcaatact ttcggtactg gaacggttcc ggaagaagaa ctgattgctg
     1681 cagtgagaga aaacttcgat cttcggccag caggaatcat cgagatgctt gatttgcgtc
     1741 gtccgattta caaacaaaca gctgcttacg gtcattttgg tcgtacagat gtagatttgc
     1801 cttgggaaca tacggacaaa gtagatgcgt tgaaagcaag tttagcaaaa taagttatat
     1861 tcagctggaa cgatcgccaa gtggtggttg ttccagcttt ttttatggca gttaacctct
     1921 tgaaaaagat agtaattatt tatctatttt tagatcctgt cataatgaaa gggaggaata
     1981 agatgaaaaa agaaacaaat gttactttgg tcacagtgag tattttcatt gcaacgttca
     2041 tgacggctat tgaaggtaca atcgtaacaa ccgctatgcc aacgattgtc ggttcattac
     2101 atggaatgga aatcatgaac tgggtatttt cgatttattt gctgacaaac gctatgctta
     2161 ctccgattta cggaaaactt gcggataaag tgggacggaa accaatcttt atttttggga
     2221 cattattgtt tattatcggt tcgttatttt gcggtttatc tcaaagcatg attgggttga
     2281 ttgctgctcg tgcgattcaa gggatgggtg ctggggcaat gatgccagta gctttgacga
     2341 tcatcggcga tctttattct attgaaaagc gagcaaaagt tttaggtttg aatagttcag
     2401 cctgggggat tgccagtgtt tttggaccgc ttgcgggagg attcatcgtt gaaacgatca
     2461 gctggcactg gatctttttt atcaatgtgc cgatcggaat tctattaatt attttgattt
     2521 ggctatattt aaatgaacca aaaatcatac atgagtctaa accaatggat atttttggta
     2581 gttttagttt gatggccatg cttgtctcat tgctgttagg gttccaatta ttaggagacg
     2641 aaggaatgag tccgcttgtt tctgctttgc tgctaggtag tatcgtaatg ttaggtattt
     2701 ttgtttttgc cgaaaaaaga gctaaagatc ctgtcatcat gatgcattta ttcaaaaatc
     2761 cactatttgt tgtggtcaac ttggctgcag cattggctag tggttttttg atgggaatcg
     2821 atgtttatat ccctatgtgg atgcaaggtg tattagggct gaatgcagca attggtgggc
     2881 tggtattagc accattatca ctagtctgga tggtcggttc tttccttgca agcaagtgga
     2941 tgactcaaat gaaaactcat tgggtcttga gaattggact actaatcgca gctattgggg
     3001 gcgcttggtt gtatcttatg cctttcagaa gtaactattt atggtttttt gctatatcaa
     3061 cggtcatcgg tataggctta ggtgtgacca tgactacctc gactgtcagt gcacaaaata
     3121 gtgtatctcg tgaccagctt ggtgtagcaa cttcttttaa tacgttagtt cgaacaattg
     3181 gtcaaacagt catggtttct atttttggtt tattgataaa tggggtaact aaacgagaac
     3241 ttgcagcagc aaatttaaca aatgatgcag atatcatgaa taaactggtg aatccacaaa
     3301 cggctaaata tattgatgcg gatattttgt taccattaag aaaaattctt tatgatgggt
     3361 tacattccgt ttatctagtt ggcttgttac tcgtgatcat tgctttggtc ttaacgcttt
     3421 tcgttcatga aaaaagtggt aaaataaaac agtagtttcg gttaagatat gtacgtagcc
     3481 gaatcattca aatttaaagt tattcaaaaa taacttctta ctaaggaagg cgaaaatatg
     3541 gcatttattc aagcaaatat ttattcgaat gtgttagaaa tggaagtcaa tctaaatgtg
     3601 atactaccgc aaacaacaaa aaaggtgatt ggcaccagtt cagagcaaga gacaacggat
     3661 atacctgttt tatatttact tcatggaatg ggtggcaatc atagtgtatg ggaaagacgt
     3721 acgtcgattg aacgttatgt agcaaactac ggattagctg tcatcatgcc atctactgat
     3781 cttggctggt acacagatac agtttatgac atgaagtatt ggacattcat tgcagaagag
     3841 ctacctgaga tttgtcatga attattcccg caattaacaa gaaaaagaga aaaaacatat
     3901 gccgctggat tatcgatggg cggatatggc gcgttaaaac taggccttgc aaaatcagaa
     3961 aatttcgcag cagtaatctc tctttcaggc gctgtttctg taggaaatcg aatggatgat
     4021 cttttgatgg ttcgtaaagc atctttttgg gaagggatct ttggaccatt ggatcaaatc
     4081 gaaggttcaa aaaatgatcc gcaatactta ttgaaagaac tagtaaaaag cggaaaacca
     4141 gttccaagat tttatatggc ttgtggggaa tctgattttc tttatcaggc aaatcaggaa
     4201 atggtatctg ctatgcagga acaaggcttg gcggtcacct ttgaacaagg acctggcgat
     4261 cataactgga tcttctggga ccaatggatt caacgtgcat tggcgtggct aatggaagaa
     4321 aaataaaaaa agagtcttaa aaagatagct cctatagaga tcacaaacat agtgatctct
     4381 ataggagctt ttttactgct cgttttctct tcttttattt tgttggcata agccttcaat
     4441 ataggtttgt gcactcgtta gaaagagtgc tttttcattc aattctgcat gggactgcat
     4501 actttcttta aatccctcta attgattttc aactacataa ttacgcataa atagaactgt
     4561 ttctttcaaa aattcatttc cgtccataat tttttgtcga agctgctgtt catgctgctg
     4621 atccattaac tgtccaatca ataaattgtg gagagaggca atggcctgat gactttgggt
     4681 gactgaaaat ccttcacgaa caagtaattg gatcatttgc tccaaatgtt ctaatcgact
     4741 cggaaaagcg ggcacagtat tgatttctaa ttctgcccca caagggaaac gtgtataaag
     4801 gtcatagtaa ttttccatat actgcagcaa ctgttcattc caaggcaaag ttggatctgg
     4861 aagcgctaat tcttcttcca ttgtttcagc cattttttgc aagagctgtt gtttgttttt
     4921 gaaataccaa tataaagcag gtgcttgaac atgaattctg tttgcgatgt ttcgcatgga
     4981 aagctgcgaa agagcttgtg tttcagccag gatatcaaag gcggcctgaa tgatgattgc
     5041 ttgagaaagc tttatttcca ttttatcaac ctcgtgtatt gcattatctt tttaaacatg
     5101 gtaaattgtt agtcggtcta attttacagt gttaaattag tcctgtcaat ttaaacaaag
     5161 gggtgtaatc aacgaatgaa attaatctta cgacatgcga aaaaatataa agtagctgtt
     5221 tttatttcct tgctgtccgt agcagtcatg gttgcagcag cattatggca acctaagtta
     5281 ctacagcaag tattggaagc tattattaca gaagataatg atgaaatgaa aacaattggt
     5341 atttacctga tcagtctggc tttgctagga ttagccgcag gtgtaacgaa cacgattttc
     5401 tctgcaaaag tagctcaagg cgtaagtgca gatatccgag aagaagcatt tcgaaagatc
     5461 cagacatttt catttggaaa tatcgaacag ttctctgctg gaaatcttgt tgttcgtcta
     5521 acaaatgata tcacgcaaat ccaaaattta gtgatgattt ctttgcaatc actttttcga
     5581 attccattct tatttattgg tgccttcatt ttagctatga tcacgatgcc gcaattatgg
     5641 tggatcatcg tagcgcttat cgttacggta tttctgatta cggcactttc atttacaagg
     5701 atgggcaagc acttcatgat cattcaaaaa ctgattgatc gtgtgaatag cattgcaaaa
     5761 gaaaacttac tagggattcg tgtagtgaaa tcttttgtac aagaagaaaa ccaattgaag
     5821 tcattcagca aagtaagtga ggacttaaca aaacacaaca tcatagtcgg tacattattt
     5881 tctgtgatga tcccgtcctt catgctggct gccaatttgg ctgtggttgg ggcgatcttt
     5941 tttgtgagcg atcttgttaa agatgatcct acgttaattg gtggaattgc ttcattcatg
     6001 aactacttga tgcagattat gatggcaatc attattggcg gaatgatgat gatgatgact
     6061 tcaagagcag ccgtatcgct gaagcggatt gcggaaattt tagatgctga accagatctt
     6121 acttatttag acgttccaga acaagagttg acgggttctg tcacttttga ccatgtttct
     6181 tttcgttatc ctggtgagga tacagatacg cttaaagata tcagcttttc tatcaaacct
     6241 ggagaaatgg ttggtatcgt cggggcgact ggtgccggga agtcaacact agcacaattg
     6301 atccctcgtc tatttgatcc gacagaaggc aaagttgaag taggtggtgt agatcttcgt
     6361 caagtaaatg aaaagagttt aagacaaacg gtttcctttg ttttgcaaaa agcaatttta
     6421 ttctcaggaa cgattgctca aaacttgcgc caagggaaaa agaatgcaac agaaaaagag
     6481 atggaacatg cttcttctat tgctcaagca aaagaattca tcgaaaagtt atctgatcgt
     6541 tacgaagcac caatagaaga acgtagcagt aacttttctg gtggtcaaaa gcaacgttta
     6601 tccattactc gtggcgttat cggtagtcca aagatcttga tcctagatga cagtacgagt
     6661 gcactggatg ctcgctctga aaagctggtc cgagaagcac tcgatcgaga tttgaaggga
     6721 acaacgacga ttgtcatcgc acaaaaaatt gcttctgttg tcaaagcaga ccgcatctta
     6781 gtattggatg aaggcagatt agtcggcgaa ggaacccatg aggaattggt agctggaaac
     6841 cgtgtctatc aggaaatctt tgaaactcaa aaaggaacgg aggagtaagt atgactgatc
     6901 taataaaagc aagtaaattt ttctatcact atttgaagcg gtacaaactc tcctttttct
     6961 ttatctttat cacagtgatc attgctacct atctacaagt aaaggcacct caatacatcg
     7021 gggaagcttt tcaagaatta gctaattacg taggaggctt gatgcaaggc gtagacgata
     7081 aaagcaaatt tgtttctgta atatggaaac tcttgctgtt ttatgtacta gctagtgcag
     7141 ccaactttat ctatagtatt ttgttcacac aagtagtagg gaagtcaacg aaccgaatgc
     7201 gtatcggtct attcaacaaa ttggaaaaat tgacgatccg tttctttgat tctcatcaag
     7261 acggtgaaat tttaagtcgt tttaccagtg acttagataa tattcaaaat agtttgaacc
     7321 aagctttgct tcaagtcatc acgaatgctg ttttacttgt cgggatcttg atcatgatgt
     7381 tccgccaaaa tgtagagtta gcttgggcta caattgcttc cactccagta gccgttttga
     7441 tagctgtctt gattatccgc aaagcacgga aatatgtgga tcttcagcaa gatgaagtgg
     7501 ggaaactaaa tggttatatg gatgaaaaaa tcagcggaca acgtgtcatc atcacaaatg
     7561 gcttacaaga agaaacgatt gaaggattct tagagcataa tcaaagtgtt cgcgaagcaa
     7621 ccttcaaagg acaagcttat tctggtctac tatttccgat gatgcaagga atgtcactgg
     7681 tcaatacagc gatagttatc tttttcggcg gctggttagc attaaatggt gatttggaac
     7741 gttcggtggc attaggctta gttgtgacat tcgtccagta ttcacaacaa tattaccaac
     7801 cactgatgca gatttcatct ggatacagca tgatccaatt agcgattaca ggggcaagac
     7861 gattgaatga aatatttgat gaaaaagatg aagtcaaacc agaaaacggt aaagcatttg
     7921 atggtgttga aaaaggtctt gcgttgcagc acgtagattt tggttattct ccagaaaaac
     7981 tagtcttgaa agatgtctcg atcaatgtgg acaaaggaga aatggttgct cttgttggtc
     8041 caacaggatc ggggaaaacg acgatcatga atctgctaaa ccgtttttac gatgtcaatg
     8101 atggttcagt agtcatcgat ggaatagata tccgagatta tgatcttgat gcattacgta
     8161 gacatgtagg aatcgttttg caagaatctg tattgttctc cggaacgatc aaagacaata
     8221 tcacattcgg caaaccggac gcttcagatg aagaagtcat caatgcagca aaacaagcga
     8281 atattcatga cttcatcatg acgttagaaa aaggttatga tacagagata tccgaagaaa
     8341 ataacttatt cagtacaggt caaaagcagt tgatcagtat tgctcgaacg atcatcacaa
     8401 atccatcttt attgattttg gatgaagcaa caagtaatgt agacacggta acagaagcaa
     8461 ggatccaaaa agcaatggat gaagcaatca aaggaagaac cagctttgtg attgctcacc
     8521 ggctgaaaac aatcttgaat gcagatagaa tcgttgtctt gcgtgatggg gaagtcattg
     8581 aagaaggcag tcatcatgag ttattagcag aaaatggatt ttatgcggaa ctttatcata
     8641 atcaatttgt atttgaaagt taaattaaaa gaacctcgtt ataacggggt tctttttata
     8701 tattttatgt tgtttgtttt tataaggggc a
//
LOCUS       DACDIE010000089         8617 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.090, whole genome shotgun sequence.
ACCESSION   DACDIE010000089 DACDIE010000000
VERSION     DACDIE010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8617
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.090"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..467
                     /locus_tag="IV250_002501"
     CDS             <1..467
                     /locus_tag="IV250_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769740.1"
                     /translation="FNQKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKE
                     HIIKHEAKIGSQWVNLPYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKE
                     QVNANGLVQISGVKGVADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            642..1475
                     /gene="msrA"
                     /locus_tag="IV250_002502"
     CDS             642..1475
                     /gene="msrA"
                     /locus_tag="IV250_002502"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP8769741.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            1555..2544
                     /locus_tag="IV250_002503"
     CDS             1555..2544
                     /locus_tag="IV250_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HAP8769742.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            2833..3531
                     /locus_tag="IV250_002504"
     CDS             2833..3531
                     /locus_tag="IV250_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAP8769743.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            3681..4997
                     /locus_tag="IV250_002505"
     CDS             3681..4997
                     /locus_tag="IV250_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8769744.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            5014..6429
                     /locus_tag="IV250_002506"
     CDS             5014..6429
                     /locus_tag="IV250_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8769745.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            6894..7214
                     /locus_tag="IV250_002507"
     CDS             6894..7214
                     /locus_tag="IV250_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8769746.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            7240..7557
                     /locus_tag="IV250_002508"
     CDS             7240..7557
                     /locus_tag="IV250_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP8769747.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            7586..>8617
                     /locus_tag="IV250_002509"
     CDS             7586..>8617
                     /locus_tag="IV250_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8769748.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVL"
BASE COUNT         2863 a         1388 c         1806 g         2560 t
ORIGIN      
        1 atttcaatca aaaaataatt tctcagccgg gacatattgt tgtaaacaaa gaaacagtag
       61 aggcgtatac cactaaagga atggattatt ttttatatga ttattctcct atcgcttcat
      121 tggagaataa ggagcatata ataaaacacg aagcaaagat tggcagtcaa tgggtaaacc
      181 ttccttataa aacaactggt agttctatca tatatagagt aactaatgag aagagccaag
      241 taatcacact tcctgtatat gcttatttag gaacgaatgt agaaataaat catcaacctg
      301 taaaggagca ggtgaatgct aatggattgg tacaaataag cggagtcaaa ggagttgctg
      361 atattaaagt aagcacaagt tatacagtaa cgactattat ttcttatctt gttacattaa
      421 ctgcatattt atgtttattt tttaaaatag ttttccataa aaggtaaatt aacttttcat
      481 ttataaaata aattgattaa aaaggagaat ctgtcgataa ggattctctc ttttttatac
      541 gaaaaatctg tagggggatg aagtaatcgc ttatttcctc actttatgat aaattacagc
      601 taaagtcact agcttgtctt cttcggaaag gaggagaatc tatggatgag gaacaaatat
      661 tcattcaaac actttataat ctgattttga atccgaatac acgagattgg gaacgaaaag
      721 tattgattca gacaaagaat gacataagag aaaatatatc cgttaaagaa cagttatcaa
      781 aactagaagc aacactacgt ccattagcta tccgaatgaa tctgacgcct gatgtaatgg
      841 acttttattt attgattacg gaaggatttg ataaggagca aaaatatgac ttttccaagc
      901 atgccatgca agatgctgac tatcaagaac gtgcagtatt tgcaggggga tgtttctggt
      961 gtatggtcga gccgtttgaa tcgaaaaaag ggatcctatc tgttttgtca ggatacacgg
     1021 gcggacatgt tgaaaaacca aattatgatc aagtaagtgg tggttacaca ggtcatgtgg
     1081 aagctgtgga gatcatttat gacacaagag aaataagtta tagcgagttg ctgacaatct
     1141 attggcaaat tactgatccc acagatacat ttggacaatt tcaagataga gggaagcaat
     1201 atcgtccagt gattttttat caagtcgaac gacaaaaaga attagcggaa caatcaaaac
     1261 agaaactaga cagttcagga actttccacc aaccaatcgt cacaaaaatc gaaccagccg
     1321 gtacgttttg gccagcagaa aactatcatc agcaatttta caaaaaacaa ccgaaacgtt
     1381 ataaaaaaat ccagcaagca cgtaatcaat ttctgatcta tcaacgagta aaaaacaaat
     1441 ggcaaaaaaa tatacgaaaa aatcattttg attaactcct tttctttaaa atgtagtaaa
     1501 ctaaaaatag aaaaaagaaa gcgttttaaa aagatttgat gaggaggaga aaaattgtat
     1561 attgcagata gtgcacgtta tgaaaaaatg gaatatcgta aatccggtca ttcaggtttg
     1621 aaattaccga ttttatctct cggattatgg caaaattttg gtgattatga tcctatccat
     1681 aatcaaagag agattttaag aggcgcattc gacatgggaa ttacccattt cgaccttgcg
     1741 aataattatg gaggccctgc tggagctgca gagaaaaact ttgggcgtat ttttagagaa
     1801 gattttcaag cctaccgtga tgaactgatt atttctagca aagcaggtta tcatatgtgg
     1861 gaagggccat atggggaatg gggctctcgt aaatccatca tttcaagttg tgaccaaagt
     1921 ctccaaagaa tggggctaga ttatgttgac atcttctatc atcatcgccc agaccctgat
     1981 acacctttgg aagaaacagc agaagctttg atgcaacttg taagacaagg aaaagcatta
     2041 tatattggta tatccaacta taatggagaa gatacaaaaa aaatgactga aattttaaaa
     2101 agaaaggaag caccgttcat tattcatcag atgcgctaca atatgttttc tcgtgcacta
     2161 ctagaagacg atctatctcc agtattagaa gaagaagggc taggggcaat cacgttcagt
     2221 ccgcttgctc aagggttgct gacaaatcgt tatctacatg gtattcctga agattctaga
     2281 gcacatcgta aggaaatacc attcctttca gaagaacaag ttggatcaac tctggaaaaa
     2341 atcaaagcac tgcaaacaat cgccgtatca cgaggacaat cattagcgca aatggccctt
     2401 gcatggaatt tgcgacaaaa atctgttaca agtgtactgg tcggagcaag tcggttaagt
     2461 cagttacaag aaagtgttcg aatgatggat aatttagatt tctcaccaga agaagaattg
     2521 agaatcgacc aaattctaga atagaaacga tgtaatcaaa ctttttatcc atatattaaa
     2581 aaaaacaaaa gatgacatca agaatcgaat gaagatcgtc tgttattgaa catcatattt
     2641 tttgtcgttc aaaataaaaa tgatcgaagg ctaggagtga attttatcct agtctttttt
     2701 atggtaaata tcttcttcgt ttgtatgtct atgaacggat taatcaattt tgtatacaaa
     2761 aaatcatcag atgattcaaa cgtgttataa taattacgaa aaaatacatc agatgaaaag
     2821 gaggtatgtg gtttggaaga aaataaaacc ctagtagaag taactactga caagatcctt
     2881 agatatttaa aggaaatgca gttgcaagta ggagataaac tgccgaatga atatgaattg
     2941 tccgattatt tagaggtggg gcgaagcacg gtgcgagaag ctgtgcgggc tctagcttcc
     3001 aggaatatcc ttgaagttcg gcaaggagcg gggacatttg tcagtgataa gcgagggatt
     3061 gccaatgacc cactcggttt ttctttgatt gaagatcagg atcggatgat acgagactta
     3121 tttgaactga gatatttatt ggagcctaag atggcaggtt tggcagcgga atatgcaacg
     3181 aagaatcaaa tcgtccaaat gaaaaaatta acgcaaaaga ttgaaacttc ttttcgagaa
     3241 ggcacacaag aacatgttca attagacatc gattttcata cgatgattgc agaagctagc
     3301 ggaaacgtag ccttgctgca tattatccca atcattaatg aatcaattgc tttgtttaat
     3361 aaaaattatg atatccagca tctgaaaatc gagacgatcg atattcatac agagatcgtt
     3421 caggccatcg aaaggcggaa tgcacttgct gcgtttgatg caatgactgt ccatatggca
     3481 ataaatcgga gagaattaca gaaaataata ggagaaaaag aaactaattg acagttcatc
     3541 tgatgtattt tatgataaat acatcagatg aatttttgtt ttatatttgg taaaaaagag
     3601 gagtaaatag tttctgttca tcgaaatgta agcgctgact aatagacaaa aagagatgac
     3661 gtcaaggaga agaagcagga atgaggggat ctggaatgag ttttatggat aaattagcca
     3721 atgttttagg gaacgtggca acgagaatca ataatttaag gtatatcatg gtgataaaaa
     3781 acgcatttgc tgcattgatt ccggtaatta ttacgggtgc ttttggcaca ttgttttcag
     3841 caatggtctt tgactcggaa aatggcttag cacagataag tttcttgcgt tttttagaag
     3901 aattaaagcc tatcgcatct gccatcagct atgtcacact aagtttcttg accatctatg
     3961 ctgtttttct gatagggatc gaattagcca agctgaataa agtggatggt gtattccctg
     4021 gtatcatcgc agtgatgtct tatttatctg ttaatccaac tgtttatgaa ttagtgaatg
     4081 aaaatcagac tgttatagta gaaaacgtat tggctaaaca atatacagat acgaaagggt
     4141 tatttttggg aatgttggtc tccatccttt ctattgaact ttattgctgg ttgaggaaac
     4201 agaagaaact acagctgaga atgcctgata ccgtgccaac gaatgtttct gctagttttt
     4261 ctgctttatt cccgacgatt ctgactgtat cgggtatcgc ggctgcggga tttatcatca
     4321 aagaacttac aggaatgtac ctttatgata tcatttacaa tgtcgtacaa cgcccattag
     4381 aaggtgtcgt acaagggtta ccgggtattt tgctattgat gtttattgcg caagtctttt
     4441 gggtgattgg tatccatgga aatcaaatgg tgaagccgat acgggaaccg ttgcttttag
     4501 cggcaattgc tgtaaatacg gaagcttttg aagctggaaa agaagtaccg aacattatta
     4561 ctatgccatt ttgggatatg tatatgagta tgggcgggtc tggagtcacg atcggtttac
     4621 tgattgcagt attgttagtt ggaaaacggg aagatatgaa acaaatcacc aagttatccc
     4681 ttgctcctgg gatcttcaac atcaatgaac cagttatttt tggtatgccg atcatgctga
     4741 atccaatttt agctattccg tttatcttaa cgcctcttat cactggaacg attggttatt
     4801 ttgcaaccgc tttaggattt gcggcgaaag cagttgtaat ggtcccttgg ccaatgccgc
     4861 cgatcgtcaa tgcttatctg gcaacagcag gaaatatcgg tgcagttatt acgcaacttg
     4921 tttgtattat tgtttctatt ttgatctatc tgccatttgt aaaagtagca aatcgtgtgg
     4981 aaaatgatgg gatctaaaaa acaggaggaa atcatgaaaa ttactattcc aaaaaatttt
     5041 attcttggtg ctgcatcttc tgcttggcaa acggaaggtt ggaaaggaaa aaaagaagga
     5101 caggattcgt ttatagatag ctggtataaa aatgaacatt ttgtttggca taatgggtat
     5161 ggcccagccg tagcaactaa ttttatggaa caataccagc aggatgtgga tctgatgaaa
     5221 tccatccatc tgacacatta tcggacttct atcaattggg ctcgtttttt tactgattat
     5281 gagaactgta tagtagatga agaatatgct cagcatataa gtgaaatgat cgactctttg
     5341 atcgaagcag acgtagaacc gatgatttgt ttggaacatt atgaagtccc agcttttctt
     5401 ttggaaaaat atgatggctg gtcttcaaga aaagtagttg atttttatgc taagtatgct
     5461 caaattgcct ttgatcgtta cggtgatagg gtaaaaaact ggtttacatt caacgaacca
     5521 atcgttcccc aaacgcgttg ctatttggat gctgttcgct ggccgcatga acaaaacaca
     5581 aaaaagtgga tgatgtggaa ttatcataaa gttttagcaa cggctaaagc agtagagatt
     5641 taccgcagcg gaaattatgg tggaaggatt ggctgtattt taaatcctga aatggtctat
     5701 gctcgatcca attctccaga agaccaaaaa gctgcttata tgtatgattt gttttataat
     5761 cgagtatttt ttgatccgat ggtaaaagga tactacacag aagagttcct ggagttgtgt
     5821 caaaaacaat cgatttattt cgatcctgca acagaggatt tagaaacgat tcagcatcat
     5881 acagtagatt tccttggaat caatcaatat tatcctaaac gcgttcgcgc ccctcgttat
     5941 caatggaatg aagagacacc tttccatcct gaacgatatt atgaagtctt tgatttacct
     6001 gggaagaaaa tgaataattc caggggatgg gaaatctatc cgaaaatcat gtatgatata
     6061 gccatgtatc taaaagaaaa ttatcataat atcccttggc tgataacaga aaacggtatg
     6121 ggaagagaat atgaagaaca gtatatggat gagaaaggaa tcgtgcaaga tgattaccgt
     6181 atcgaattta tcggacagca tttgcattac ttgatcaaag caacagaaga aggcgcaaat
     6241 tgcgaaggct atatgttatg ggcattcact gactgtgtgt caccaatgaa tgcatttaag
     6301 aatcgatatg gattagttcg aatcgaattg aacgatgaac gaagcagaac attgaaaaaa
     6361 tctgcttcgt ggtatcagac tttgattgat aagagagaat tggatttgcc tgacgaaatc
     6421 tataaataaa tagaagtttt ttgtagatgt tcattgtatt tactggttat ttgaaataaa
     6481 agaggttgtg aatccagcgt tttgagctaa tacccagggt cagctgattg aacaccgttt
     6541 attgagaggt tgtgaaaaag ctgtcctgca tcaagtaata agaatagcaa agtaaaaaca
     6601 acatcttatg tttttaactt ggttggactt attaccgcag atgcaagctt ttgaataccg
     6661 tttatttgaa aaaagttgtg acaagttttg tgtcacaact ttttttgtgc acgttaaaag
     6721 aaaaaaggta ataaaaatta atttgtgtgt ttagtgtgcg ctttcttttt ttagtgtgta
     6781 tgcaacttgg tatagacctt aaatgtgatg atatcaactt tttaaagcgc ttttaaagtt
     6841 ggcacgctac ttgcttatat acaagtgtaa gtatagaaag gaacgtgttt aaaatggaaa
     6901 agaaaacaat catgcttgta tgttcagctg gaatgagtac aagtctccta gtcacaaaaa
     6961 tgcaaaaggc agcagaagca aaaggaatgg aagcagacat ctttgctgta tctgcttcag
     7021 atgcggataa tcaactagaa tctaaaaatg tagacgtttt acttttagga ccgcaagtac
     7081 gctttatgca aaatcaattt gctgaaaagc tagcacctaa aggcattcct ttagatgtaa
     7141 tcaatatggc tgactatggc atgatgaatg gtgaaaaagt actcgaccaa gcagaaaaac
     7201 taataaacaa ataataaaaa ttatcggagg atacaataaa tggaagatca aaaaaatcta
     7261 gaagccatca tgggtctgat catgtttggc ggaaatgcaa aaagcgatgc aatggaagcg
     7321 attgctgctg cgaaaaaagg tgatttcact ttagcagatg aaaaaatcaa agatgcagag
     7381 gaatctttag tccaagcaca tcactctcaa acagagatgc tgacacaaga agcacaagga
     7441 aatcacgttg aagtcacgct tttgacggtc cacagtcaag atcatctgat gacatctata
     7501 gcttttacag atttagcaaa agagatcatt gatctgtatc gtcgattgaa cgcgtaatct
     7561 acagtaaaga aaagagggaa gatttatgga taaatttgtt gcatttatgg agaaacattt
     7621 tatacctgta gcatcaaaaa ttggtgcaca acggcattta gttgcgattc gggactcatt
     7681 tatggtgagt atgccgttaa tgatcttagg agcattagca gtaatgatca ataacttacc
     7741 gattcccggg ttccaagaat tgatgaactc aatttttggt ggtgaatcat ggaaaggatt
     7801 cggggctgct gcttggaacg gtacatttgc cattctttct gtcttgattg ctttcttgct
     7861 tgcttatcat ttagctaacg gctaccgaaa agacggtgtt tccgcaggtg tgatttcttt
     7921 aggctctttc ttcgctttag gcggagcatt aggtatgagc tcaaacggtt tgtttattgc
     7981 tattattgtt gggattattt caacagaaat ctttgtacgt ttaagcggaa atgaaaaatt
     8041 gatcatcaaa atgcctgatg gtgtgccacc ggcagttgca aaagcatttg cttcattact
     8101 accagcaatg atcactatct ctgcgtttgc tttagttgct gcaatttttg ctgcattcgg
     8161 tgttgatgat atcgtaggtt ctttctatac agtggtccaa gaaccattta tgggacttgc
     8221 aaactcttat ccatctgcct tactgcttgc atttatcaca ccattcttat ggttctttgg
     8281 tttacacgga gcgaatatgg tcgatccttt gatgcaaaca atcaatgcgc cagccattga
     8341 agcaaacgtt aatgcaatcg cagctggaca tagcgcaccg tttatcgtga acaaaccttt
     8401 ctttgacagc tttgtcaact taggaggaac aggtgcaact ttaggtttac tgttagctat
     8461 ttatttagta ggacgtaaaa acaaaccata tatggttgta acaaacttgt cgattgcgcc
     8521 aggtgtcttc aatattaatg aaccaacgat gtttgggtta cctatcgtgt tgaacccgat
     8581 catgttcatt ccatttattt tgacaccaat ggtctta
//
LOCUS       DACDIE010000090         8545 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.091, whole genome shotgun sequence.
ACCESSION   DACDIE010000090 DACDIE010000000
VERSION     DACDIE010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8545
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.091"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            30..509
                     /locus_tag="IV250_002510"
     CDS             30..509
                     /locus_tag="IV250_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8769749.1"
                     /translation="MVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITNDLKLSTSMIHL
                     IDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNKKTKLVSLTKY
                     GRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEYQVSLTRKED"
     gene            602..1764
                     /locus_tag="IV250_002511"
     CDS             join(602..878,878..1764)
                     /locus_tag="IV250_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP8769750.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLTPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2044..3024
                     /locus_tag="IV250_002512"
     CDS             2044..3024
                     /locus_tag="IV250_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8769751.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3146..4564
                     /locus_tag="IV250_002513"
     CDS             3146..4564
                     /locus_tag="IV250_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HAP8769752.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4635..6104
                     /locus_tag="IV250_002514"
     CDS             4635..6104
                     /locus_tag="IV250_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8769753.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6114..6581
                     /locus_tag="IV250_002515"
     CDS             6114..6581
                     /locus_tag="IV250_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP8769754.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6617..7489
                     /locus_tag="IV250_002516"
     CDS             6617..7489
                     /locus_tag="IV250_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8769755.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7571..>8545
                     /locus_tag="IV250_002517"
     CDS             7571..>8545
                     /locus_tag="IV250_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP8769756.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLG"
BASE COUNT         2945 a         1284 c         1739 g         2577 t
ORIGIN      
        1 tggtacaagg aatagaaaga ggtgtttatg tggttgataa aattcagttt aatcaagtta
       61 tagaagaatt catcaaagta aataatttaa tgacccatat tcaaaaacga ccaatcagaa
      121 taactaatga tctaaaactc agtacgagta tgattcattt gattgatgtg attggaaatt
      181 atcctgaaac tagtattacg gaattagctg atcgattagg cgttaccaaa ggagctgtat
      241 cccaacagat tcccacttta gagaaaatga atcttataag catttctcaa aaaaaaggaa
      301 ataagaaaac taaacttgta tcgttaacaa aatatggtcg aaaggtgtac gaatcacata
      361 attatttaca tgaagagctt tatagctcaa taaatgaagc gttagaatca ttctcaccag
      421 accaaatttc aaccattaaa cagattctag aaaaagtatc ttatacaatc aatgaatatc
      481 aagtaagtct gacaagaaag gaagattgac atgaaaaaaa tgatttttga atcggctaca
      541 ctagactaga cagaaaaaat aaggtgtgta aaataaatga aaacacactg gaggaatttt
      601 tatgtcaaga cgtcaacgta gaacctattc aaaagaattc aaacaacaag tcgtcgatct
      661 ttatctcgca ggaaagcccc gagctgaaat cattcgagat tatgagctga ctacctcggc
      721 ctttgataag tgggtcaagc aagcgcaaac tactggctcg tttcaggaaa aagataactt
      781 gacccctgaa caagtagaac taattgcatt acgaaagaaa aataaacagc ttgaaatgga
      841 gaatgacatt ttaaagcaag cggcgctgat attgggacga aaagacaagt aattgatacc
      901 aataagcaca aatactcaat atcagcgatg tgtaaagtgt taaacatctc ttgccaaacc
      961 tattattatc aagccaaacc agttgaagat gaatcagaac tggaagaact cgttcaagaa
     1021 gaatttattc gcaatcgaaa agcttatggt accaggaagc tgaagcagtg cttggccaat
     1081 ctaggtcttc aagtgagtca acgcagaatc ggccgtatga tgaaacagcg tggcttgcgt
     1141 tcaacataca cattggctca tttcaaattc cattcttcta cttgtaacga agccaagacc
     1201 gccaatattc tagacggaac cttcctacag gaaaagtcat tagaagcgat tgtaaccgac
     1261 cttacctatg ttcgtgttgg gaagaaatgg cagtatatct gcctgattct tgatttgttt
     1321 aatcgagaaa tcatcggcta ttcatgcggg gagaagaaag atgcgacgtt agtcaaggaa
     1381 gcttttgcaa agattccgta ctcgttgaat aatgtacaac tttttcacac ggatcgtgga
     1441 aaagaattcg ataaccagac gattgacgaa attttatggg cctttaatat cacgcgttcc
     1501 ttaagcaaaa aaggctgccc ctatgataac gccgttgcag aatccaccta taaatctgtc
     1561 aaagtagaat tcgttcatca ataccgcttt gagacattgg ctaatttgcg attagagcta
     1621 tttgattacg tccattggtg gaataccatc cggctgcacg gcacgttagg ctatgagaca
     1681 ccgatcagtt ttcgacagca gagattggcg cagcgaatcc ttgataatga gctcggatgt
     1741 gataccggtg gcgaggcacc ttaattattt gaatgagctt ctgccttaga gaatcagatt
     1801 cgaggaggct cgttgtcaag ggcagtcgga acaccagcag caagcttacg caatacctta
     1861 taatttttgt caaaaaaagt gttgccattc cacatatatc tttaaacagc tattgccaga
     1921 taaaaataaa tgatattata tatataaata cttatccgaa aaggggaatg agagagtcaa
     1981 ggttcttgga atgtcgaata gtcgagtagg tagattttct atctacaacc ttgttgttta
     2041 aaaatgaaaa aaattggaat tagagaagtt gcgaaagagg caaatgtttc tcctacgact
     2101 gtgtcgaggg tattgaatga tcgtggatat ttgagtgagg aaacaaaaag aaaagttttt
     2161 gatgccatga aaaaattaga atactatcct aatgaactgg cgagagcact ttttaaaaac
     2221 gaaacatata ccgtaggttt gatttttcct acaattacta atccatttca cgctgagtta
     2281 attcaagata tcgagttcga gctttccagc aggggatata aagtgttgct gtgcaatagc
     2341 ttaaacaatc caacaaaaga aaaagagtac ttgacgatgt tgagaaagaa tcaggttgat
     2401 ggaattatag taggaacgca caataaagat attgcggatt atgatattcc agggctacct
     2461 attgtagcga ttgatagaag tcttggtgaa aatacagtta cagtatcttg tgataattac
     2521 aggggtgggg aattagctac tcaactactt gtaaatcatg gctgtcagaa tatactgtgt
     2581 attcggggag atagtaagat aaagctaccc gcgaatagta ggactttagc atataaagat
     2641 gttttgaata aattcatgct ggaaccgtta gtcctagaag taccatttgt aaaagaaatc
     2701 aatgaaaaaa atgaaataat taagacatat ctggaaactc atccaaatat tgacggggtt
     2761 tttgcaggag atgatttatt ggcttctttg gcgattaaca acttaggaag ttcgggcaaa
     2821 agagttccaa aagaggttaa agtgattgga tttgatggag ctcaacaaac attgatttat
     2881 aatccagaat tatcaactat tcaacagcct atagatcaaa tatctaaagt agcagtaaaa
     2941 aaattattga atatgattaa gggcgaaaag gagacggatg aactttcact tccagtaaag
     3001 ctcataaaaa aaaatacagc ataagttaaa tcggcgaaaa attccttcgt cgatttaaaa
     3061 atatttttat gaaacgggtt gacatatgaa acgggttgac atatattagt tgtgaagata
     3121 aaaacctttt cactggagga agaaaatgga gaaattatta attaaaagca ataatcatca
     3181 actttactac caacctaagg atgtttgggt cggagatgtt atgccttacg ggaaagaagg
     3241 aaaattcttt atttatcatc aacgtgatac tagaaatcca gttccttttg gagaaccgtt
     3301 tggctgggct ttagcaacta cgaaagattt tgttcatttt gaagactttg gggaatctct
     3361 tttaagaggg gatgacaatg agagagacca gtttatctac gctggttctg tatttgaggc
     3421 aaatggaaag gttcacgcat tttatacagg gtataatcgt gaattcttag cgcagggaaa
     3481 gacatctcaa gtgttgcttc atgctgagag tgatgatttt gttcattggg aaaaatcaga
     3541 aacagcattg gagttagaac cacaagaagg gtatgatatt cgaaactgga gagatccatt
     3601 tgtcgtttgg gatgatgaaa aagaagaata tttgctaatt ttaggggcgc gaaagggtca
     3661 ggataaacat ctacaaaccg gacgtttagt ggaatttaca tcaaaagatt taactaattg
     3721 ggaattcaaa ggtgattttt gggctccggg attgtatacc atgtttgaaa tgccagaact
     3781 tttcaagatt ggtgactggt ggtatcttgt ctattcagaa tactcagatg gaaataaaat
     3841 actctatcga atgagtcaaa gtttggaagg tccttggatt aagcctaaag atgaagcttt
     3901 tgatggacgg gcttattatg caggtagaac agcttatgat ggaaaacgtc gagtcctatt
     3961 tggatgggta ccaacaaaag atcaagaaaa tgataaaaac aattatttat ggggaggtac
     4021 tttggttcct catgaagtct atcaaaaaga ggatttttca cttggggtaa aacccataga
     4081 cgaagtgtgg aatgcattca taaatttaca tgatattgcc gatgtagaga tagaaacagc
     4141 agacacaaga gaagaagtaa cgcttgtcaa aaatagtgga actttgttta atttagaaac
     4201 aacaatttca tttgatgaga atcttagtga cttttcactg agattatata aaaaccttga
     4261 gacagatgaa tcttacgaat atcgcttcaa tttagatgag gggcgtctta catttgataa
     4321 aaacccttgt tacccttggt ttcaatatat ggataaagga cttattagac ctatatcttt
     4381 aaagcctaac caatcttata aactaaaagt gattgttgat gacaatataa tgacacttta
     4441 tattgatgga atcgctctta acgctcgtgt acatggttgt tttggtcaag caattactat
     4501 tacagcaaca aatggatcaa tgactgcaat aaataccaag ttttcaaatg aaataaagaa
     4561 ataataaacg tcaatcatgt ggcaggtctg aaaatcaaac atgacctgtc acatttaaaa
     4621 gggaggataa atgaatggac tacaaaaaaa ttgccgaaga tgtattggaa aacgttggag
     4681 gaaaagagaa tatctcaagc gctacacact gcattactcg tttacgttta attcttaaag
     4741 acaaaagcaa agcgaatatg gaaatgcttg aggaaataga aggtgcaaaa ggtgttcttt
     4801 tcaatagtgg acagttacaa gtaattttcg gaacaggtat aattgaaaag gtttacaaag
     4861 agttcgttga attgacagga gctaaggaag tatccgtttc tgaaatgaaa gacgaaggag
     4921 tcaataattc aggaaataga cttcaacgag cttttaaagt tttctcggat atctttattc
     4981 cgatcattcc agcttttatt ggggcagcaa tggttttagg aattcgttcg ttgattacca
     5041 ctcctggaat gtttggaatg gatggctcat tcgctgatca caatgtattc ctaaatgatt
     5101 tatccaaatt tctgggtatt attgcaacca catttgatta tttaccagtt ttagttatgt
     5161 attctgcaac aaaacgtttt ggcggaaacc ctattttagg attacttgta ggatttgtta
     5221 tgattcatcc aggactagcc aatcggaatg atttcgtcat gggaactata aagcctgatt
     5281 attggcattt atttggatta agcattccag cagttgcgtt tcaagggggt gtttttccag
     5341 caattttgac ttcttggttt ttagcaaaag ttgagaaaat tacaaataaa tattctcctc
     5401 aagtacttgc ttacatttta gtcccaactg tgacaattgt tcttgccaac tttgcactat
     5461 ttttagtgtt tggtccattg gggaatttta tcggtaatgt tttaggtgga atcattgatt
     5521 tcctatatat gaagatgggt gcctttggtg cttttgtttt tgcaggcgcg ttacaaccat
     5581 tagttgtaac aggaacacaa catgctattc aaggtattga ggcaaactta gtcgcaacaa
     5641 ctggattcaa ttatatccaa cctatttggt cagtttcaat tattgcccaa ggtggcggtt
     5701 gtattggtat gtatttactc tttaagaaaa aatctaaaga tagagatatt gcgatttcaa
     5761 gctttattcc aacactggta ggtattactg aaccggctat ctttgctgtt aatttgaaat
     5821 attcaatcgt tccttttgtt tgctcgttta ttggtgctgg ttttggtgga gcatttatga
     5881 aaatttttga tgtaaaagcc attggacaag gattgactgt attgccaggt ctgactattg
     5941 ctaatccagt tcttccttat atatttggta atctcatagc attcatttta ccaatcattt
     6001 ttattctcgc ttatgataaa tataaagtgt ttatccctga agctaaacac cgtaaatttg
     6061 cagaaaaaac tgactcaaaa aaattgccag ttcaagtaaa gtgagatgag agcatgttaa
     6121 atttttttag aaaaaatgaa ataagcgttg tagcacctgc aaccggtgtt tttgaaaaac
     6181 tggagaaatt aagtgatcca gtcttttcaa aaggaatgat gggacaagga tttgcaattg
     6241 atgcaaaaaa cgaaacgatt gtatctccaa tcgatggttt tgtttcaagt atttttccta
     6301 caaaacatgc cttaggaata aaaacaaaat caggaattga gatacttgtt catgtaggaa
     6361 tagatacagt tgagctaaat ggtgagggat ttgatattaa agttcaagaa ggacaaaaag
     6421 ttaaggctgg ggatattctt atgaccatta attttgtagt ttttgaaaag aatcacaaga
     6481 ctaaagatgt cattgttgtt tttccagact accaagacga aattttcatt ccgaaacttg
     6541 aagaagtaaa acaaggacaa gaattgtttg ttcttggcta atgataagta attaataata
     6601 gaatcgagga aaatgtatgg tttttggggc tattgaagct ggtggaacaa aatttgtttg
     6661 tgccattgga gataaagatt tgaacatatt tgaaaaagtc agctttccca cgactacacc
     6721 caaagaaaca atgaatttag ttttagattt ttttaatcat cataaagatg atttagaagg
     6781 tatcggagtg ggatcgttcg gtcctattga tattcattgt gaatctaaaa cgtatggctt
     6841 cattacatca acgccaaaac ttgcttggca ggattttgat tttattggaa gtttaaaaaa
     6901 gcaacttaat attccaactg cttggacgac tgatgtaaat gcctcttgct atggtgaata
     6961 tgtttctgga aatggcagag gcctttcaag tgttgtttac tataccgtag gaacgggcat
     7021 aggaggtggc gctatccaaa acggaaactt cattgaaggt tttagtcatc cagaaatggg
     7081 acatatgctt gtaaaacgac acaataaaga caacttttct gggacatgtc cattccacgg
     7141 agactgttta gagggcttag ctgctggtcc ggcaatcgaa agaagatcag ggaaaaaggg
     7201 acaagaagtg actgaaaatg acgttacttg ggaaatagaa gcttattaca ttgctcagtg
     7261 tgcatataat acaacattga tgttatcacc tgatgtcatt atatttggtg gcggagtaat
     7321 gaaacaaaaa cacttaaaaa agaaagtaca agataagttt attgagttga tcaacggata
     7381 tgtcgctatt cccgatgttg acaattatat tatgacacca aaattgttag ataattctgg
     7441 tattgtaggt tgtttagctt tagcaagaga cgttaaagaa aaatcttaat ttggaaaaaa
     7501 gatcgtctcg actatttgtt tggttgatgc gatctttttt ttcaattagt aattgaaggg
     7561 acgcctaaaa gtgaaaaaaa aaatgaaaga tgaagtatta gagtatatca aaaataattt
     7621 aaagtattac gatttttctg ctcaagatat tgcaatgaaa ttttgtatta aaagaaatgt
     7681 cgcttcccac tatttgaatc agttattttc agatggaaaa ttactaaaga atgattctgt
     7741 tagaccagta atgttcaagt ataatcaaca aaagccaaaa gactgctttt ctaagtttat
     7801 tggagcagac atatctttga aatccactat agataaatgc aaagctactg taatgtatcc
     7861 ccctaatggc ctcccattaa taataaaagg aaacagtggc gttggaaaaa gttttttagc
     7921 aagtttgatc tatcagtatg ctcttgatag aaaagtaatt cataatgacg caaaatttgt
     7981 tgttgtgaat tgtgcagatt atgcaaataa tccagagcta ttgtcagcag ttttatttgg
     8041 atataaaaaa ggagccttta ctggggctga aaaagatact gcagggatgc tatcttctgc
     8101 taatggagga tacctttttt tagatgaggt tcacaatcta agtgcagaaa accaagagaa
     8161 attgtttctt cttatggatt ctcaaaagta tcgtaaactt ggtgaaagtg tgcaatggga
     8221 gtatgcaaat gttcgcctta tcttagccac aacagaagat aaaaatacct ctttgttagc
     8281 aacttttaga agacgcatcc cagctgaaat cactctgcca gattacaata atagaagtac
     8341 atctgaaaaa attcaattat tatttgaatt tcttaaagat gaagcaatta caattgattc
     8401 aaaaatattt tgttcgattg aactttttac tgatctttta tcgaaaacat ttgaaggaaa
     8461 tgttggagaa ttgaggaatg aaatcaagct tctttgtgca gaaggttatt taggaaataa
     8521 gagaaagtct tcaatttatt taggg
//
LOCUS       DACDIE010000091         8493 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.092, whole genome shotgun sequence.
ACCESSION   DACDIE010000091 DACDIE010000000
VERSION     DACDIE010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.092"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..173)
                     /locus_tag="IV250_002518"
     CDS             complement(<1..173)
                     /locus_tag="IV250_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8769757.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(593..1714)
                     /locus_tag="IV250_002519"
     CDS             complement(593..1714)
                     /locus_tag="IV250_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HAP8769758.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVMDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYKPG"
     gene            2042..3451
                     /locus_tag="IV250_002520"
     CDS             2042..3451
                     /locus_tag="IV250_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HAP8769759.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /locus_tag="IV250_002521"
     CDS             3448..4176
                     /locus_tag="IV250_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP8769760.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="IV250_002522"
     CDS             complement(4304..5215)
                     /locus_tag="IV250_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8769761.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="IV250_002523"
     CDS             complement(5232..6239)
                     /locus_tag="IV250_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP8769762.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="IV250_002524"
     CDS             complement(6236..8365)
                     /locus_tag="IV250_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP8769763.1"
                     /translation="MKKKLFRIVGIVFISIVLLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..8477)
                     /locus_tag="IV250_002525"
     CDS             complement(8370..8477)
                     /locus_tag="IV250_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769764.1"
                     /translation="MQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2600 a         1726 c         1485 g         2682 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtttgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccatca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca ataggacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg ttt
//
LOCUS       DACDIE010000092         8112 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.093, whole genome shotgun sequence.
ACCESSION   DACDIE010000092 DACDIE010000000
VERSION     DACDIE010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8112
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.093"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            338..811
                     /locus_tag="IV250_002526"
     CDS             338..811
                     /locus_tag="IV250_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAP8769765.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            808..2940
                     /gene="feoB"
                     /locus_tag="IV250_002527"
     CDS             808..2940
                     /gene="feoB"
                     /locus_tag="IV250_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAP8769766.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            2942..3076
                     /locus_tag="IV250_002528"
     CDS             2942..3076
                     /locus_tag="IV250_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAP8769767.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            3170..3814
                     /locus_tag="IV250_002529"
     CDS             3170..3814
                     /locus_tag="IV250_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8769768.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            4001..5194
                     /locus_tag="IV250_002530"
     CDS             4001..5194
                     /locus_tag="IV250_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP8769769.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            5187..6914
                     /locus_tag="IV250_002531"
     CDS             5187..6914
                     /locus_tag="IV250_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8769770.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            7308..7505
                     /locus_tag="IV250_002532"
     CDS             7308..7505
                     /locus_tag="IV250_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterocin"
                     /protein_id="HAP8769771.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            7507..7818
                     /locus_tag="IV250_002533"
     CDS             7507..7818
                     /locus_tag="IV250_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP8769772.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPGPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            7922..8068
                     /locus_tag="IV250_002534"
     CDS             7922..8068
                     /locus_tag="IV250_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HAP8769773.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFSLF
                     KKCN"
BASE COUNT         2701 a         1339 c         1761 g         2311 t
ORIGIN      
        1 aaagtcaaac aacaaacact acatattgtg ttgtggaaac tgtggaatac taagttttgt
       61 gtttttgaaa aaagagctga aaacaagtat cgtgtgggaa aatagcaaat ttaatagaga
      121 aaaaattgtg gaaaactatg aagaaatatt tttttctcat ttttatctat caatactaaa
      181 gattcgctca taatatcaca agtataattg acattctcaa ttaccgagag tattctatgt
      241 gagaatgatt ctcatttatt tgcgtaacac gtaagaaaat tagaacatat aatgaatgat
      301 tttaatacat gatttatttc acaagagagg aatcaatatg cagaccttag cgcaggcaaa
      361 gcagaatttc gtttatcaag tcgaacgaat cgaaacagaa gaaaaaacaa aacgccattt
      421 gcaaaatatc gggatcgttg ccggaagtaa aatcgtcgtc gtcaatttat caggcaacaa
      481 tgccatttta ctggtaaaaa gtcatcgagt ggcagtaagt cgggaaatac taggtcaaat
      541 ctttgtcaat aaaaagactg cttcctctga aagctggact tctcttgatc aattaaaagc
      601 aggagagact gggatcgtcg tagcaatcca cggacaaggc gcagtgaagc gacggttaat
      661 ggatatggga ttgacgcgaa atgtaaaaat agttatccga aaactagccc ctctgggaga
      721 tccaatcgaa ctgacagtgc gagggtatga actgacgtta cgaaagtcag aggccgaact
      781 aatcttgata caaagggagg aaaaagaatg aatatcgcgt tagtcggaaa tccaaacagc
      841 gggaaaacta gcttgttcaa tctcttgact ggttcgaatc aatatgtagg gaattggccg
      901 ggcgtaacag tagaaaaaaa agaaggatcg ttgaaaaaaa atccgaacat tcatattcag
      961 gacctacccg gcatctattc tttgtctcct tatacaccag aagaagtgat tgcaagagat
     1021 tatttggttc aggaacagcc tgaactgatc atcaacatta ttgatgctac caatttagaa
     1081 cgaaatcttt atttaactac gcagttattg gatttagaca ttcctatgat cattggtctg
     1141 aatatgatgg atgtcatcaa aaaagaagga aaaaaaatca atctagaaaa gctttcctat
     1201 ggtcttggtg taccagtttt accaatcagc gtattgaaaa agcaaggaat cgaaaaacta
     1261 atcgaaaaga ttcaggagtc tgcaaaaaag cggccttcta ttcctgttcc ctgtacatat
     1321 gatccacgtt tagaagcagc tttacatgaa attgaagatg tactaggaga acaaagcggc
     1381 aagaaacgct ggtatgcaat gaaatatttt gaacgagatc agaaggtaag agaggattac
     1441 gagatagcag tttctcagca aaaagagatc gagcaactga tccagttaac agagaaacta
     1501 ttggacgacg atagtgagac gattctggtc aatgaacgtt atgaatttat tacccaacta
     1561 tgtacactct gtgttgtgtc aaatgattct ttccaattat cgatgagtga caaaatcgat
     1621 cagattgcaa cgaatcgttg gttagccttg ccgatcttcg catttgtgat gtggctgatc
     1681 tattatctgg ctattcaaac agtaggaaca atggggacag attggatcaa tgacacattg
     1741 tttggtacat ggttaccaga acatgttggc agattgttag ctgaatggca agtagcggga
     1801 tggatgcagg agctgatctt gaacggcatc atcgcaggcg tgggagcagt gttaggattt
     1861 ctaccgcaat tgattatatt gtttttatgc ttgagctttc tggaagactg cggatatatg
     1921 gcgaggatcg cttttgtcat ggatcgactt ttcaggaaat tcggtctttc aggaaaatcc
     1981 ttcattccta tgctgattgc tacaggatgt ggagtgccag gcgtcatggc cagtcgcacg
     2041 attgaaaacg agcaagatcg tcgtttgacg atcatggtca caaccttcat gccttgttct
     2101 gcaaagttgc cgattattgc cttagtagca ggggcctttt ttcctcatca atcttgggta
     2161 gctccttctg cctattttct tggtatgact gcaattattt tctctggaat cagcttgaaa
     2221 aaaacacgtt tgtttgctgg agatacagct ccttttatca tggaattgcc cgcatatcat
     2281 tttcctcaat ggtcagcggt cctaagacaa acctatgacc gcagcaagtc ttttgttaaa
     2341 aaagcaggaa cgattatctt tgtttctagt atcgtgatct ggttcagctc ttcttataat
     2401 ttttatgctc aacctgtttc tgaagaccaa agtatcttag cctttttcgg gcgaatcctg
     2461 gcagttgttt ttcagccgct tggttgggga aactggcaag gaacagtcgc agcgattaca
     2521 ggattggtcg ctaaagaaaa tattattggc acatttggta ttttgtttgg tcatgcggaa
     2581 gtatccgaaa atggcagaga aatctggcaa gtgctacaac acacatatac tcccgcagca
     2641 gcttattcat tgttagcctt caatttactt tgtgcgcctt gttttgcggc aattggggct
     2701 atccatcggg aaatgaatgc caagaaatgg acttggatcg ccataggcta tcaatgcggt
     2761 ttagcttatt tagttagttt tgtgatttat cagttgggac acttattgga gggtggacag
     2821 gtagcgttag ggacgtatct tgctatttta ctgatgatag gactcgttta tgttttgttc
     2881 cgtcagccaa aatccaagaa ccagttttat actatattgt cattagaagg agaggaatag
     2941 gatggcgaca ttcttattaa gtgtgttaat ctttggaagt gcaggcgcaa ttgtctatac
     3001 tcgtttaaaa tccggtaaga attgtgatga ctgtcaaaca gcttgcccag taaaaaagga
     3061 acagatcaag cgatgatcag tcgttgcaag aggctattct cccctttttt gcttattttg
     3121 ctagagcata gtaagatgag ggaaagtaaa aaaagaaaga ggaaagaaaa tgtctggaaa
     3181 gaaaaaaatc gtccgcccgc gctaccagca gattgctgtt gatttagccg aacgtatcgt
     3241 tgaaaaccga tataaagtgg gtgagaagat ccatgcccgc tcaacacttg ccagtacgta
     3301 caatgcttct cctgagacta cccgcaaggc tatcaatgtg ctggtagatc ttggaatcat
     3361 ggaagtacgg catggaagtg gggcatttgt tgcttcgaag gaaaaagcaa aagatttcct
     3421 attgcaatat caagatgtac aatccatcca agatacaaaa gcagaaatca tggcaagtgt
     3481 ggagaagcaa caagatgagc tgaatcattt ttctcaatta ttggatcagc tggtcaatca
     3541 aacgaagcga atacataaga tgaatccttt acttccgttt gaattatctt tatcagaaaa
     3601 agcaatgcat ctggaccgtt caattagcga gttgaatatc tggcaggcga ctggcgccac
     3661 agttatcgca atttctcata atgaagaact gcttgtttca cctggcccct atgctaaatt
     3721 tacggcaggg gacatcgttc attttattgg aaatgaatat acactacagc gtatgacaaa
     3781 ctttttttac ccggagcatc agcttgcaga atgattgtgt gaaatattaa aaaaatatta
     3841 acttggctaa accttttatt tacaaagatt tagccaagtt tttttcagtt tggacaagca
     3901 aatttctttc atttttgaca aagtgactac atgatgttaa catagtgtgg tcactttttt
     3961 cagaaagaaa aatgaattta cataaaaaag gagaatttgt ttggtaaaag tagaagtaaa
     4021 acatttaaca aagatatttg gtaaaaagac acaagccgct ttagatatga tgaatgatca
     4081 ccagccgaaa acggagattt taaagaaaac cggtgcaacg gttggtgtgt atgacgtgaa
     4141 ttttgacgta aaagaaggag aaatcttcgt gatcatgggg ctttccggga gtgggaaatc
     4201 cacattgatc cgtctattga atcgtctgat tgagccaact tcgggcagta tctatattga
     4261 tggagaagat gtatcgaagt taagcaaaga agaattgcga gaagtccgtc gtcataaaat
     4321 caatatggtt ttccaaaact tcggtctgtt tccacatcga acgatcttgg aaaatacaga
     4381 atacgggtta gaagtccgtg gtgtacctaa agaagaacga caagaaaaag cagaaaaagc
     4441 attagaaaat tctagtttgc tttcatttaa agaccaatat cctagtcagt tatctggtgg
     4501 gatgcaacaa cgggttggcc tagccagagc tttagcaaat gatcctgaga tcttgctgat
     4561 ggacgaagcg ttctcggctc ttgatccgct gatccggcga gaaatgcaag acgaattact
     4621 ggacctgcaa gccaatgttc aaaaaacgat tattttcatt acccatgatt tgaatgaagc
     4681 tttacggatt ggggacagaa tcgccttgat gaaagacggc gaaatcatgc agatcggaac
     4741 aggtgaagaa atattgacca atccagcaaa tgattatgta cgtgaatttg tggaagaagt
     4801 cgatcgttcc aaagtgctta cggctcaaaa catcatggta cctgcattga cgacgaatat
     4861 cgaatctgat ggaccaaacg ttgcattgac aaggatgcgt aatgaagaag tcagcatgct
     4921 gatggctgtt gacagaaaga gacacctgaa agggatcatc actgcagatc aagcgttaga
     4981 agctaggaag caaaaacgtc cattgatcga cttcctagat gaaaatgtga cggtgattgg
     5041 gaaagacatg gtggtaagtg atattttcaa tattatttat gactcaccga ctccacttgc
     5101 agttgtagag gatggaaaat taaaaggtgt cgtcatccga ggtagtgtga ttgaagcatt
     5161 agcagaaaca agcgaggtga gtgaacatga ataatctatt gaactatcaa ttaccagtgg
     5221 caaactgggt agaaaatatc acagaatggt ttacgacaac tttttctggc ctattttcat
     5281 ttttacaaac gattggacag gcagtgatgt cggggattac aaatttactg ttagtgattc
     5341 cagctccttt gtttatcttg ttattgacgg tagcagcttt ctttatttct aagaaacgtc
     5401 caggacttac gttgttcaca ttaatcggtc tatggtttat ttataaccaa ggtctatgga
     5461 atgatttgat gaatactgtc acacttgttc ttttatctag tgtgatctcg atcatcattg
     5521 gggtaccatt agggatcttg atggcaaaaa gcagtaaagc ccaaagtatt atcaaaccga
     5581 tccttgactt tatgcagaca atgccaggat tcgtttattt gattccagca gttgcatttt
     5641 tcggtatcgg gatggttcca ggggtatttg cctcagtcat ctttgctctg ccaccaaccg
     5701 ttcgttttac gaatttaggg attcgacaag ttcctaaaga attagtagaa gcttctgatt
     5761 cattcgggag cacaagtcgt caaaaactat tcaaactaga attgccttta gctaaaagta
     5821 cgatcatggc agggatcaat caaacaacaa tgctgtcact ttcaatggtc gttattgcct
     5881 ctatgatcgg tgcacccggc ttaggacgcg gcgtattatc agcattgcaa agagcacaag
     5941 tcgggaatgg atttgtcaac ggggttgcat tagttatctt agcaatcatc attgaccgtt
     6001 ttacacagca tttgaaccag ccaaacaata aaaaagctgc aggagcaaca acacagaaaa
     6061 gtaaaaaacg tcaaggactg atcattggtg ctgtagtagt agtgatctta ggtgcgattg
     6121 gtatagggag tttttcatct gccaaagaaa caaaacgtat caacttatct tatgttgaat
     6181 gggatacaga agttgcttca acaaatgtag tcggtgaagt attgaaacaa atgggctatg
     6241 atgtaacgat gacgccatta gataattcaa tcatgtggaa atctgtttca aatggcgagt
     6301 cagatgccat ggtttcagct tggctaccta aaactcatgg ttcacaatat gcacaatata
     6361 aagatcaagt cgaagaccta ggtgccaatt tgacaggagc aaaagttggt ttagcagtgc
     6421 cagcctacat ggatgtaaat tcgatcgatg agttaaccga tcaggcaggt aagaaaatca
     6481 taggaatcga gccaggagct ggtgtagtta cggctgctga aaacacgatc caaaaatatg
     6541 acaacttgaa agactggaaa gtagagacat cttcttcagg agcaatgacc gttgcactag
     6601 gtcaagcgat caaaaaacac gaaccaattg tagttacagg atggacacct cattggatgt
     6661 ttgccaaata tgatctgaaa tacttagaag atcctgaaaa cggaatggga agcgaagagc
     6721 agatccatac gatggttcga aaaggattga aagaagacca gccagaagct tacaaagtat
     6781 tagacaattt ccattggtca gaaaaagata tggaaaaagt tatgttggaa atcaacaatg
     6841 gaaaagatcc acagcaagca gcaaaagact ggatcaaaga gaaccaagaa ttagtcgaaa
     6901 gctggaaaaa ataaaaagac agacacgaaa taaatggagc ttccaaaatt ggatcaaata
     6961 tccaaatttg gaagctccat gttcgtctct atcttttttg tctataaaaa aagagaatgc
     7021 gtagctcatc ttcgaaacgt ccaaagacgc cgacaaacta ttatgaattt atgggtgcga
     7081 gtcaaagaga tcgatcgtag gtatgaaaga ctaatgtgat taaagtttat tatgtacgaa
     7141 gtgcattctc aaaaaatatt aaagaatata catagtttat atacttaaaa gtgattaaat
     7201 tcttattcag gaattatttt gctacgtttc aagaaggaat tgttttttga aatttaatga
     7261 atgttagaat aatagtctaa aaacattaat ttaggggtga ttagattatg aaacatttaa
     7321 aaattttgtc tattaaagag acacaactta tctatggggg taccactcat agtggaaaat
     7381 attatggaaa tggagtgtat tgcactaaaa ataaatgtac ggtcgattgg gccaaggcaa
     7441 ctacttgtat tgcaggaatg tctataggtg gttttttagg tggagcaatt ccagggaagt
     7501 gctaaaatga aaaaaaatgc taagcaaatt gttcatgaat tatataatga tatatctata
     7561 agtaaagatc ctaaatattc tgatattctt gaggttttac aaaaggtata tttaaaatta
     7621 gaaaaacaaa aatatgaatt agatcccggt cctttaataa atagattggt gaattatcta
     7681 tattttactg cttatactaa taaaataaga ttcactgaat atcaagagga attaataaga
     7741 aatttgagtg aaattggaag aactgctgga ataaatggtt tatatcgagc agattatgga
     7801 gataaatctc aattttaatg atacttataa tattaaatat gtctcaaaat tttttgatta
     7861 gaaaaatagg tatattgaat cagtgacata aaagtattta tagtttgtag ggggaatcgt
     7921 aatggaagaa aaaaatagac taaatgctaa acaatgttca gatcaagagc ttaaaaaaat
     7981 taaaggaggt gctggaacaa aacctcaagg aaaaccggca tcaaatttag tggaatgtgt
     8041 tttttcatta tttaaaaagt gtaactaaaa ttttttttat gatatcctaa aatcaggagg
     8101 ggatctcttg at
//
LOCUS       DACDIE010000093         7889 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.094, whole genome shotgun sequence.
ACCESSION   DACDIE010000093 DACDIE010000000
VERSION     DACDIE010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7889
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.094"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            172..516
                     /locus_tag="IV250_002535"
     CDS             172..516
                     /locus_tag="IV250_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAP8769774.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            517..936
                     /locus_tag="IV250_002536"
                     /pseudo
     CDS             517..936
                     /locus_tag="IV250_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
     gene            complement(1028..1378)
                     /locus_tag="IV250_002537"
     CDS             complement(1028..1378)
                     /locus_tag="IV250_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769775.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1344..2675)
                     /locus_tag="IV250_002538"
     CDS             complement(1344..2675)
                     /locus_tag="IV250_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAP8769776.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2931..3497
                     /locus_tag="IV250_002539"
     CDS             2931..3497
                     /locus_tag="IV250_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8769777.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3642..3729
                     /locus_tag="IV250_002540"
     tRNA            3642..3729
                     /locus_tag="IV250_002540"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3674..3676,aa:Ser,seq:cga)
     gene            4063..5037
                     /locus_tag="IV250_002541"
     CDS             4063..5037
                     /locus_tag="IV250_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAP8769778.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            5362..7257
                     /gene="asnB"
                     /locus_tag="IV250_002542"
     CDS             5362..7257
                     /gene="asnB"
                     /locus_tag="IV250_002542"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAP8769779.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEKWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(7452..7886)
                     /locus_tag="IV250_002543"
     CDS             complement(7452..7886)
                     /locus_tag="IV250_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAP8769780.1"
                     /translation="MKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIAIDQA
                     KKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKSKGAK
                     KASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
BASE COUNT         2752 a         1323 c         1508 g         2306 t
ORIGIN      
        1 ataaaaaaag cgttagcaac tatgcttaaa aagcatatcg actgaataaa ctaagcattg
       61 gacaggctga aagactacat ttaaaatgaa atgcgaagaa acaatagaaa ataaagttgc
      121 cttagaacca tagttgaatc acctaacagt cgtgaaggga gaataagaga aatggcagaa
      181 aaacaaaccg caggacgaga tcaattagga gattttgcac caaaatttgc agaattgaat
      241 gatgatgttt tatttggaca agtatggtcc agagaagaat tatctgctca tcagcgtagt
      301 atcatcacca tatctgcatt aatttcagga ggaaattttg aacaattgcc agctcatctg
      361 aagattggca agcaaaatgg cattactagt gaagaaatag cagaaatcat tacccattta
      421 gctttttatg taggatggcc aaaagcttgg tcagctttta gtttagcaaa agaaatctat
      481 ttagaagaaa atagcgaaga taagaaagag gcgtaaatga cgacaaaaca cgaagaaata
      541 cacaatggcg tgatttttcc agcaggtgaa aaaaacgaag cttacgctca agtatttacc
      601 gggtaaagct atctgaatat gttagtgaat gatcccgaag tttctgttgg tgtaggtaat
      661 gtaacttttg aaccaggttg tcgcaatcac tggcatgtcc ataaagacgg ctatcaaatc
      721 ttacttgtga ccggaggaga aggctggtat caggaagaag gaaagccagc aagacgatta
      781 atggctggag atgtcgtcgt aacgcacaaa gggattaaac attggcatgg tgcgacaaaa
      841 gacagttggt ttgaacattt ggcgatcact gctggtaatt cagagttttt agaacctgta
      901 acagatgaag tgtatgaagg attggaaaat caataatgtc aagcttgaac agctcctgaa
      961 agtagatttt aatcgaactt tcaggagctg cttttttgat tcagtctttt ataaatagaa
     1021 aatagggtta ataataggaa tgagaagcac gtcgaaagat cttaatattg ataatcggct
     1081 gggcatacat gatttccacg tcatcaatca aactacctaa cagatgataa tcttcccgat
     1141 catgtcgggg attacctaat agttcttgat aatagatttc catactaggg tcccgaggat
     1201 tgttcagcaa atcagcaagt acatctaaat cttctggctg agtttctgcc gtaccctgta
     1261 tctctatgaa aagcccttcc tcagcaaatt ctactccaat acgtatttca gttatttcat
     1321 gggaatataa ataagtaaga agctcatcga tgattctttt tgataattct ttttccaagt
     1381 gtcttttttt cctttctgac agcggtaaag ttatctagta tcggtacaag gatacctgca
     1441 acgaaaccag ttgaaaaacc actgttatat agatttagtc ctccgtgcaa agtgctgacg
     1501 ttatggacaa gagcaatatg tagtacacca gctattacac cgtaaaaact gccgtaaaaa
     1561 ccgcttatgg gcgcgagacc tgtcccaaac acagctgcaa caagcatgcc agtactggtg
     1621 atctcatgaa aagttccaaa caaagaagca ataaatattc cgactagtat cggaaaactg
     1681 ttttttaaat gacaaccaaa agcactgaaa cccaccgcag ataaaatagc accgatgaca
     1741 ggtccattta actgaccgcc aaccagcaaa acataagaag acagcatcag accaactaag
     1801 cctatgttga tcaaagtagc accaatacca gaattagcaa tgaaatcagt tgtcaatttt
     1861 ccagatgaat cgaaaatttc tcgaatcccc aaaagccgaa aggaattata atagaaacca
     1921 attccgactg tcagcgaaaa aaagccaaaa ataatccaaa tgagaggaaa gtgatattca
     1981 ttgaaaacca atgtattttc gacgattgaa tagctgaata acctcaaaac agcagtaaaa
     2041 gtcatcgcaa tcaacccaga ggtgaatcca atattataaa gtgtgaatcc acgatggaaa
     2101 actaaagttt gagcagctaa gggcggaaga agtagaccaa ttgctatgcc agctaaattc
     2161 cccaataaaa tacctatcag taaaggaaaa cgcataccaa aagtgatata actaacgacc
     2221 ggtgataatg cgctaccaaa cagtccgatc atgatgtact gagaaaaagg tttattgaca
     2281 aatacagcgt atagatacac acctaaaatg attgagatag aattataaag gtttttgcca
     2341 aaaaaagaga aaccggtaac tgttagaatc gaagcaaata agggaccgtt taaaatcacc
     2401 ttgtgccgat aagctaaaag cacagaaagt aaggtcaaaa tccctacatt tatgaatgcg
     2461 cttccgaaac cgcctaaagc aaaataatct gtcaaaagat tgcttggcga ggtaagaata
     2521 gttagcaatc cactccaatt atcagtagga gtattagaaa gcaagccaat aaaaagaaaa
     2581 aaaacacctg taaaaccaag ataaaaatac tttcttttct gtgaaacgat tcgtttttga
     2641 ggatgaatat aaataacgtc agtttcgttt tccattacac accttctttt tcattttctt
     2701 taactataac ttgaaatgaa agatttggac aactatcatt taggtcaaaa ctaaaaatgt
     2761 aagggaaccc attgaattta accaaatttt tttaattttg caaaaaaaag ttaaagaaga
     2821 ctgaaaaaaa atttaaaata taacgttttg agtaaaatgt ttgcaaaaaa tgctaaaaat
     2881 agggtacaat aaaacaacat atactgcagt aaagaagtgg gggagtttta atgagaagaa
     2941 aagtctatac caaggatcaa attctaaaag ctgcttatga tgttgttgca aaggaagggt
     3001 ttaaaggatt tactgcgaga aatattgcta aaaagatggg gatttcgaca cagccgattt
     3061 atctggaatt caaaaacatg gaagatttaa aaaatacgtt gctggattct atctttgaac
     3121 aactaaaaga agatgttttc cctattgaac gtactggtga taaaatcatt gatctcggtt
     3181 tgaactatat ctactttgca aaaaagcatc atagtcttta catggcattg tacattgatg
     3241 aacatgggga cggaaagaaa atgcatgatt tttcatacaa ttatttccgt gaaatagtta
     3301 aagaagatga aagatacaat caattatcgg atgaatatgt cgatgctctg catactggta
     3361 catgggtagc agtgactggc ttagcgtcat tgatgtcatc aaacatcatg catccaacag
     3421 aagaacaaat cattgcactg atcaaacgga caattgactc gattttagca ttagataatc
     3481 ctgaaataat cagatagtgt tctatcagta tgaatttgat cggtaagtcc gttggctgca
     3541 aactgtcaat ggactttttt gctaaaaaaa gcgatttctg ttatttatct attagaaata
     3601 ggcttgacat ttcgtgaaaa aagtttaaaa atagtactta cggaaggttg gcagagtggt
     3661 aatgcaccgg actcgaaatc cggcgaaccg gctaataccg gcgcgcaggt tcaaatcctg
     3721 taccttcctt atatcaacga ttagaggttg tgaagaagct gtcctgcatc aagtaataag
     3781 aacggccaaa caaaaatagc ttttcctatt ttttcgtttg attgggctta ttaccgcaga
     3841 tgcaagcttt tgaacaccgt ttgttcggaa taaggggctg tgacaagact tttgtcacag
     3901 ccccttattg ttgtactttt aacctacaat attttttgat tccccctgag tactattttt
     3961 tataaatgct agtaaatcaa tcttctttgg cctttttttg ataaaaaaaa tactagatgt
     4021 aagataaaag tgtaaaaacg atttgtttta ggacgggaaa cgatgtgtac cgcaatcact
     4081 tatgcaacga aagaccatta ttttggaagg aattttgatt atgagatgtc ttataatgaa
     4141 gtggtcacta ttactccaag aaattatcgt ttcgatttta gaaaggtaaa aaatttagat
     4201 aaacattatg caatgatagg aatagcagct ggggtatcca actatccact ctattatgag
     4261 gctacaaatg aaaaagggtt gagtatggca ggactgaatt ttcctggcaa tgctgattat
     4321 aaagaattac aagaaggaaa agataacgtt gcaccatttg agtttattcc atggatacta
     4381 gggcaatgct caaccataga cgaagctaaa gaattattgg ctactatcaa tctagtcaat
     4441 atcgatttta gtgaaaaact acccttgtca cctttacact ggttattagc tgataaagaa
     4501 aaatcgatag taattgaaag tatgaaagat ggacttcatt tatatgataa tcctgtcggt
     4561 gtgttgacaa ataatccgcc atttgattat caattattta atctgaacaa ttatcgctcg
     4621 ctatcaaatg gaacaccaga aaatcatttt tcaaaccaga tcagcttgaa tgtttatagt
     4681 cgtgggatgg gaggattggg cttacctgga gatctctctt ctgtttctcg atttgtaaaa
     4741 gccacattta ctaaaatgaa tgctgcatca ggtgattcag aatctgaaag tatcagtcaa
     4801 ttttttcata tactaggctc agtcgagcaa caaaaaggag tatgcgatac tggagaaaga
     4861 aaatacgaat atacgattta ttcttcttgc tgtaacgttg ataaagggat ttattattat
     4921 cgtacgtatg aagacagtca aattactgct gtaaatatga ataaagaaga cttggatcgt
     4981 catgaattgg tcagttatcc agtcataaaa gagcaacaga taaactatgt caattaatta
     5041 atgaatagat ccgtttggta tcatattttc tgatacagtc ataattagac acgatcttta
     5101 ggtcgtgtct ttttttgctg gtttttttgg aaatagcatc cactaatatt tttttaacac
     5161 gaagagaata aggtagcctc aaaaaagaaa acatggtaaa ttagatattg ggactttatt
     5221 ttctgtaaaa acagaaactt tcatcttttg ttttcacgtg acgaaattgt aagagagaaa
     5281 atattcgtcc gctatttatt ctatgataag atgcatatga atgaaataaa cagtaatttt
     5341 atattaggat gtgaaagtta aatgtgcggt attgtaggtt ttgttaacga caaggacaac
     5401 aaaaaaacaa ttattaatac aatgatggat cgaatcgttc accgtggacc taatagttca
     5461 ggagaatata tcgataaaca tgttgcttta ggattcagaa gattaagtat tattgactta
     5521 gagggcggta ctcagcctat ttacaatgaa gatcggacaa agatcattat ttttaatggc
     5581 gaaatctata attaccagcc tttgagagaa gaattgattg cagctggcca cgtttttcaa
     5641 acacatgcag atacagaagt ccttttgcat ggttacgaag aatggggaac agaattattg
     5701 caaaagatcc gcggaatgtt tgcttttgct atttgggata atgaaaagaa tgagttattc
     5761 ggtgcaagag accactttgg gatcaaacca tattattatg cagaaatgaa cggaacattt
     5821 atgtttggtt cagaaatcaa aagttttctg cctcatcctg attttaacaa agaattgaac
     5881 aaagaagcgt tgaaacctta tatgactttc caatattctc cattaaacgg cgaaacattc
     5941 ttcaaagatg tctatcgttt gcctgaaggc cattattata cgtataaaga tggaaaaata
     6001 gacattcagc aatattggga tgcagatttt gaaacaaaag aaacgcattc ccgacaagaa
     6061 tggatcgaaa aaatcgacga aaccgttcaa gcttctattg aagcacatac ggtcagtgat
     6121 gtagaagttg gttctttctt atccagcggg gttgattcca gttatgtaac ctctgtattg
     6181 aaaccagatc attctttctc aatcgggttt gatgataaaa cctataatga agcgattgaa
     6241 gccagaaaac taaccgaact attggactta gataatacag cagcagtgat cgacggagac
     6301 atgtcgttca aagcattccc tttgatccag tatcacttag atgaacctga ttcaaatcct
     6361 tcttgcgtac ctttgtactt tttagcaaat cttgcttcac aaagtgtgcg ggtagtccaa
     6421 tctggagaag gggcggatga actgtttgcg ggataccaaa cgtatggatt ccatacgaat
     6481 tcaaaattca tccgtgtgat tgctcaagga ttgaaaaaat taccaaaagg aacacgctat
     6541 aatttaggac gtaaaatcgg aaaaatgaaa aatttccatg gacggatcca tttatatgaa
     6601 tcacttgctc ctgcaaaaga atattttatc ggtcatgctc gagtgttcga agaatcagaa
     6661 gcatcagaag tgttaacacc aaaatatcaa acagcaccat ctgtggatga aatcatgact
     6721 gttcattacg agaaaacaga aggaatcaaa gatgaagtca ataagatgca atatgtcgat
     6781 cttcatcaat ggatgccaaa agatatcttg ttaaaggcag ataaactttc tatggcaagt
     6841 tctttggaag tccgagtacc tttattggat atcgaagtca tgaaattggc tcaacaaata
     6901 ccaagtaagt ttttattgaa tcaaaacaac acaaaagata ttttcagaca agctgcgaac
     6961 aaacatcttc cggaaaaatg gtcgaatcgt gtgaaattag gattccctgt gccaatcaaa
     7021 gcttggttga aagaagaaca cggctacgaa caagtcaaag cattgttcga agcagatttt
     7081 gcaaaagaat tttttgatca agaaaaaatc atgaagttgc tagatgacca tcacgaagga
     7141 cgtaaagaag agcaaagaaa aatctggaca atcttcagtt tcttgacatg gtataaagtc
     7201 tactttgtag atgaaacgat tccgcaagca gaagcaatcg actatgtgac tgtttgatga
     7261 aaatcaaata tcaataaaaa agaggttttc tcctaccgtt taacactgag tgataagaaa
     7321 caatttcaaa aaacagctct tcccgtttta tagatattat gacttatggt tcggagttgg
     7381 cagaggaaca ccattgtttc gaacataagg gggttgtgac acgattttcg tcacaacccc
     7441 tttacatatg ttcaataatc gatttctttt tcttttgtcc catctttttt ataaatgaac
     7501 aaagttgatt ctttgttttg tgctacttgc tttccttttt cgattgcttc tttttttgta
     7561 tcaaaatggt tgcttgcctt ttttgcccct ttagatttca cgatccattg ttcttcctca
     7621 tattctacga tcacatctga atcaagcagt tttccagcac gaggattcgt atcgtgttca
     7681 tccgtcttgc ttggattttt ttctttttcg aaagttcgtt tttctgattc actagcatct
     7741 tcataccatt tttttgcttg atcaatggca attgggatcg cacggttatc agggtaccct
     7801 tcatctaata atgcatttgc gatgtcgatt gctttttttc ttgtcaattt atctaagttt
     7861 ttcattgatg caggaaagtc cttcatgtt
//
LOCUS       DACDIE010000094         7614 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.096, whole genome shotgun sequence.
ACCESSION   DACDIE010000094 DACDIE010000000
VERSION     DACDIE010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7614
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.096"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            69..1496
                     /locus_tag="IV250_002544"
     CDS             69..1496
                     /locus_tag="IV250_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP8769781.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1588..1953)
                     /locus_tag="IV250_002545"
     CDS             complement(1588..1953)
                     /locus_tag="IV250_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP8769782.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(1950..2315)
                     /locus_tag="IV250_002546"
     CDS             complement(1950..2315)
                     /locus_tag="IV250_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP8769783.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2507..2755)
                     /locus_tag="IV250_002547"
     CDS             complement(2507..2755)
                     /locus_tag="IV250_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769784.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2754..2882
                     /locus_tag="IV250_002548"
     CDS             2754..2882
                     /locus_tag="IV250_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HAP8769785.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2939..4633
                     /locus_tag="IV250_002549"
     CDS             2939..4633
                     /locus_tag="IV250_002549"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HAP8769786.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4700..4906
                     /locus_tag="IV250_002550"
     CDS             4700..4906
                     /locus_tag="IV250_002550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769787.1"
                     /translation="MFLIWLDLLPQMQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFL
                     TWLDLLPQMQAFEHRLPRGYEKGV"
     gene            5284..6417
                     /locus_tag="IV250_002551"
     CDS             5284..6417
                     /locus_tag="IV250_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAP8769788.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6448..6768
                     /locus_tag="IV250_002552"
     CDS             6448..6768
                     /locus_tag="IV250_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769789.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6797..7261)
                     /locus_tag="IV250_002553"
     CDS             complement(6797..7261)
                     /locus_tag="IV250_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8769790.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7393..>7614)
                     /locus_tag="IV250_002554"
     CDS             complement(7393..>7614)
                     /locus_tag="IV250_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8769791.1"
                     /translation="VSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAA
                     VNLVLSILIYLPFVKVATIQERRKEALDA"
BASE COUNT         2492 a         1468 c         1371 g         2283 t
ORIGIN      
        1 tttcaaaaag aaaacatgtc ataatatttc ccatattgtg caaaaaaata caaggcggag
       61 gctctactat gattgaagaa tatattgaga aaaatatttt aagacagctg tttttatgtg
      121 ggcaattcta tgtaaataaa gaagtcaatc tagaaaagct ctccaatctc ctgcatgttt
      181 gcaaaaccac tttactcaat gatatcaata acataaaaaa agaatttgaa gaacagatcg
      241 cttatacaca tcgggaaaag gaccgttaca ctttatattt ttcagaacat atacctcgat
      301 gcaagatcat gcagcaattg tctcaaaatt cgttgttttt aaaaacttgt ctgctttatt
      361 tggaagaaga tgaacctgat tatcttcaat taactgaatg tgaattcatc tctgtcagca
      421 aagcttattc attaaaaaaa caagtattag cttattttaa tgactgcggg atcgaaatcg
      481 accgctactc ccctcgattc actgaaatgg aaagacgtct gctgcttctt aatgtgtcgt
      541 atcgtttagg cggttttaat tcttgggagc tacctgaatc gttttttgaa cgtgccgatc
      601 gtttcattga atccgtaacg gaaaatagtg gcagatttta tgataaagaa aataaagaaa
      661 tcttatcgat tggttttgct atcagtttcc tacgccagca agtgtgtgct gtgacaatag
      721 attcaaaatt tatcgaagaa atcaaaaaac gtcctattta caattatgtc gaatcagcat
      781 gggaaaacac tgatttccaa acctattata aaaaagaaga attcgccttt attttgacac
      841 tattcaatct ttgtaattat ggtttccatt cttatcagtt gattgcggaa gactttcaac
      901 agctacacca agtgtttatt gacaatactc ccgaaataaa agaactagtt gctacctttg
      961 aatctcactt caatcaagaa ttattcggaa accaaccgtt cgaacgtgcg ctgattcatc
     1021 tgatgcgttc ggcttgggat aactatcaat tattcatgcc agaaaaattt tatcttttaa
     1081 atgaggaaca gacaaattta tataaagaag tccagacaat tttttccagc tggtccagcc
     1141 agttacctta tgatctacgg ttgaatccta attgcatgcg ggcttttgtg atcgaattga
     1201 gcggaattct ccgtctgaca aaagaacatc tgactatcta tatcgtcaca aattcggatg
     1261 ttcattatct gatttatcgg gaagccttgg aagctgtaac tactttcgat tttcaagttg
     1321 caccaacaat ttattcaagt atatctgata taaagaagta tgcgcagcag tcctcaaacc
     1381 gggtattatg tgaacgtaca ttatatacac cagatgcggt ccaatatgaa aatattattc
     1441 ctatttcgat caatacgatc gatcgagcta tcatttcagc tgtgcaaaac aaataagggg
     1501 ctgtgacaaa aatcatgtca cagcctctta tttgttgaag atgcccattt ctgtaacaat
     1561 ctcttgatac acgtcaggct cacaacctca cttattttgg acatctacgg gtccgatctc
     1621 tttatttaaa aaataagaaa tgatggttcg gataactacc aacgccgcta gttggaaaat
     1681 atcctgcaag gtaggtttga taatcgattc gatgatccca gctgaaacaa gaatttccaa
     1741 acttagaagt atatagcctc ccaacatctt tttaatagca ttgttctcgc tgactcggtc
     1801 gctttgcact tttgtatgga caaataaata tttcaaaaaa tccttgattg atagacaaac
     1861 accccaaacc aataaacaga tagcaaagat atcaagtccc agtacaagtt tttcgaaata
     1921 tggaagaaga aactgcatca actctgtcgt catcctacct ctcctcttct ttgtttttct
     1981 ttgtcttcta tttcctgatt caagaaataa gaaatgactg ttcgaatgat aataataagt
     2041 gcaagcttca aaatatctgt ccaagtcggc ttgataactg actcgatgat atcagcaacg
     2101 actagtactt ccaagcttaa caaaatataa cttgctagca gttttcttgt atctgcatta
     2161 cgagtattac gttctgcata gtttttcttg acatgagacc aatcaaaaag tttgaataga
     2221 acaagaacga tcccgacaat taagataccg atagaacaaa tatttaaaat agcaatgatc
     2281 cattctagaa attcaattaa atttgagcga aacatatacg ctacctactt tcttttaatt
     2341 tgttcctatt cttttattgt aaaagaaagt taccttgttc gtctatttaa aagttgttct
     2401 aatgataacg gttcgcttaa atttttcttt ttcactctcc catttttctc cctccatcca
     2461 taaatcgtgg aagtatccaa aacaacgtag caagttaaaa taaggcttag tccgtcatca
     2521 gcttttcaaa gaagcactgt ctcggtgctt tttttgtacc caaatttcta aaaatagaac
     2581 catacacctc ctcaaatctt ttcttcttac ctaccctatt tttatttaca ggaaaatcag
     2641 acacttcttc ttttttgtca aaatttcctt tttcttctcg tcctatcatt tggcattcta
     2701 ataaaaacga actatccttg gatatagaaa gagaaaacaa ggaggcgttt ttcatgagta
     2761 aaaaattaat caaagtcggc atcggtttag gtttgcttgc ccttggcgct gcatatctag
     2821 gaaaaaagac aggactattc gaagatgaca gtcatttata tgatgaattc gaatcaatct
     2881 agcatcctat ttagttgaat cactacggac atacttggaa gacattggag gaatcacaat
     2941 gagaaaaagt aaagcaataa tgattggtgc cggtattgcc aatatggcag cggctgttta
     3001 tttgattcaa gaaggcaaat ggaaagggga tcagatcacc ttttattcat tggatgatca
     3061 cggttcaaat gatggtgcac cgaccgacac tgtagtggat gaatattgga ataaaaacca
     3121 tccattagaa aaccaaaaag gctatattgc tcgaggcggt cgcatgctta attaccgtac
     3181 ctatgtagat ttgatggatc tgttaagtcg aataccttct gttactgagc cagggatgac
     3241 tgctgaagaa gatacgagag attttgatgc aaaacaccga acctttgaca aagcacgttt
     3301 gatggaaggt ggcaaaggaa tcattgatgg cggaaaactg ggattcaata ataaagaccg
     3361 tattcttctg actaaactga ttgctatgcc tgattcagaa gaagaaaaac tagacaatat
     3421 cactatcgaa gaatatttca aagatgatcc acactttttt gaaacaaact tctggtttat
     3481 gtgggaaaca acatttgctt tcagaacacg tagttctgct caagaattgc gtcgctatat
     3541 gcatcaaatg atttatgaat tcactcaaat cgaacatcta gtgggcgtca atcgaacacg
     3601 ttacaatcaa tacgaaagta ttatgcttcc tttgatcgag tacttgaaag aacaaggatg
     3661 caaaatcatt ttgaaccgcc gtgtcactga ctgggaattc aaagaaacac cgatgcaaga
     3721 tgaaatcact gtgactggtt tgaaaattac caatacactg actcaagaag aagaatacgt
     3781 tgtggtcgat gaagatacag cagttatttt cactaatggg tccattacag attcagctac
     3841 tttaggagat tacgaaacac cagctgtaga aaatatggat tatggtgcag cttctagtct
     3901 ctggaaaaaa gccagtgaac gtttctacaa cttgggaaat cctgacaaat tctttgctga
     3961 tcgagatgca agcgaatggg tcagctttac cctgacgacg aaaaaccatt tgttgttgaa
     4021 tgaaatcacc cgtatcacga ctcaagaacc aggaaatgcc ttgaattcat ttatttcaac
     4081 cacaccgatc acgccgttag gccaaaaaga tgtcaatatg tctattgtcg tgcatcatca
     4141 accgcatttt acgtctcaga aaccaaatga aacagttatt tggggttatt tcctttatcc
     4201 aagacgacgt ggcgaatttg tggataaaca gtatattaaa atgaatggaa aagaaatgct
     4261 agaagaactg attggacaac tttcaaaagt agatcctgga ccagtgaata ttcgtgaaaa
     4321 agaaacagaa attttcgaca gcgtaataaa taatatccct gtctatatgc cttatgcttc
     4381 cgcgttgttc aataatcgtg ccaaaagcga tcgaccaaaa gtcattccta aacattcgac
     4441 caatctggcc tttacaggag aatttgtcga gcagccttat cagatgatct ttactgaaca
     4501 aagtgctgta cgttctggag aaatcgctgc tttccatttt gcgggtattc ctatgtcccg
     4561 tcttgtcaaa acaccgcgtt acgacaaaga tattcctact ctagctcgtg ccgcaaaaaa
     4621 aatgtttgaa taatcaagag gttgtgaaaa agctgccctg catcaagtaa taagaacaac
     4681 caggctaaaa cagcttctta tgtttttgat ttggttggac ttattaccgc agatgcaagc
     4741 ttttgaacac cgtttaccaa gaggttatga aaaagctgcc ctgcatcaag taataagagc
     4801 agccaagcta aaacagcttc ttatgttttt gacttggtta gacttattac cgcagatgca
     4861 agcttttgaa caccgtttac caagaggtta tgaaaaaggc gtttagtttc gagtatgaag
     4921 aaaaattttg tgaaaatggt tcctcacgtt tttgcataaa tttgacttaa taccgagaaa
     4981 ctgatccctt ctgtcataac ctctttttat ttatgtctgt ttctttttga aaacaactat
     5041 tttttcaacg tttactctct tgccaatcca acatcatcta acggatacaa ctatttcaat
     5101 gattaccatt acactaaaaa atcggaaaca cacctctccg aacacaaata cataagacta
     5161 tagaatgttt catttaagaa aaaaagcgtt tcattatcaa aaaggtaaga tctcctttaa
     5221 aatgaaatta tctgtagtac taataatgca gacaatttta tgaatctgaa aggagcatct
     5281 ccaatgaaag cagtcacttg gcaaggaaaa cagaaaatgg aaatcagaaa cgtagatgat
     5341 ccaaaaatat tagaaccaac agatgcaatc atccgtatca cagcaacagc catttgcggc
     5401 tccgatctgc atctttacca tcatggtgaa gcagttttgg aaaaagggta tgttgtagga
     5461 cacgaaccaa tgggaatcgt tgaagaagtg ggatctcaag ttcaaacatt gaaaaaaggg
     5521 caacgtgtgg ttatcccttt taatattagt tgtggtcatt gtcatttctg cctgaatcac
     5581 atggaaagcc aatgtgatga atcaaatcca gatcagttat atggcgggct tttcggattt
     5641 ggcaagttga acggtaatca ttggggtggt caagcggaat atctaagagt tccttatgca
     5701 gactctactt catttgttgt accagataat gatcttcctg atgaaaaagt tttatttcta
     5761 tcagatattt tgcccacagc ttattggagc gttgaaaacg caggagtaaa aaaaggcgat
     5821 acagttgtga ttctaggttc tggtccagtc ggtctattcg ctcaaaaatt cgctgttatg
     5881 gctggagcag agcgcgtaat cgcagtagat ccagtacagc accgtctaga taaagcggct
     5941 aattacaatg gggttgaaac ttatttctta gaagacacgg cacaagctgg aacagaattg
     6001 tatgaactgg ctaaaggtgg cgcagatgtc attatcgatt gtgtaggaat ggacggatta
     6061 gagcctgtca aagaaaaagc gaaaaattta gtaagcttac aaagcggcac aatctctcca
     6121 ttacagatgg catcgcaagc tgtcaaaaaa tttggaacag tccaagtaac tggtgtttat
     6181 atgactccag cttcttctta cccgctgcaa gaatttttta tgcgggatat cgctgtcaag
     6241 catggacagg caccaattat ccatctaatg ccaaaaattt acgcaatgat tgcagaagga
     6301 ctattcgatc ctacacaaat cattactcat tcgctgccac tatcagaagc agcacaagca
     6361 tatgagatct ttgacaaaaa agaagataaa aatataaaag ttgttttgaa accttgaatt
     6421 cagtaaaaaa tacaggtgat tttctttatg cccaaaatca aacgaaatac ccatgagatc
     6481 gaaataggaa aagaagaaga tatcgaaata cgaggacgtc gcttccgctt ctatttccaa
     6541 aatcgttaca cattgatctc actggctgtc gatttattaa ccgggatttt ctatattttt
     6601 ggaagccttg cttccttaac taaaatcccc gatcgatatg gcacctattt ttatttggct
     6661 ggtgctattt tcttgacgat tcgtcctatt ctcagaatcg taaggaacat ttttatttac
     6721 gacgaaaaaa aagcaatgaa gagaacaagc aaaaaaaaag aggcatgaga atttttcaaa
     6781 tctcatgcct cttttttcac cctatcgtat cactttgata cagacgagcg gtcgtcccac
     6841 tttcttcctt gaccattgtc aaaaatgtcc actcgtatcg ttcataaaac gtggtatgat
     6901 ctgtaattaa gtaaagtttt tcgtacccaa gttgatgcat ttcttttctg atttccgcta
     6961 atatccgctt ggctatcccc tgcttacgct tttccggttc aacaaacagg gcacaaagat
     7021 ttggtgtcag atcagggcgg ttatggaagt cgttttcaat gacgcccgct cctccgacga
     7081 tggtttcttt ttcatctaag accacatacc attgaggaat actttcctta ttagcgattc
     7141 cagtacgaat actttcctca tacacaacag caggaactcc ccattttgat gaaaaccaat
     7201 tcgcgctttg ttttactaat tctgggtgtt ctcttaattt gatccagtta tatgtaacca
     7261 atgtttaaac ctccgactaa aataaaacgt ttacaaaaat tatacaagtc tttatgaaaa
     7321 ttagcaatga caacaatagt gatgtaaaaa aaaaaacgga aaacaatcac ttgttctccg
     7381 ttttttgatt aatcaagcat ctaaagcttc ttttcttctt tcttggatcg tagctacctt
     7441 gacgaacggt agatagatca aaatagaaag aaccaaattg acagcggcta gtacaccacc
     7501 tgcaatactt tttgttgcca atacaccgcc aataattggt ggagttaccc atggaggcat
     7561 aacggtagct gctggcacta aacctgtaga agtcgcaaag taagcaacac taac
//
LOCUS       DACDIE010000095         7397 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.097, whole genome shotgun sequence.
ACCESSION   DACDIE010000095 DACDIE010000000
VERSION     DACDIE010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.097"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..142)
                     /locus_tag="IV250_002555"
     CDS             complement(<1..142)
                     /locus_tag="IV250_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8769792.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQ"
     gene            complement(183..437)
                     /locus_tag="IV250_002556"
     CDS             complement(183..437)
                     /locus_tag="IV250_002556"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP8769793.1"
                     /translation="MTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKEGISVSL
                     KHVQKLMKQLNLRSIVVKKYRPQRSNKPIMDRLQLTRQKN"
     gene            complement(548..802)
                     /locus_tag="IV250_002557"
     CDS             complement(548..802)
                     /locus_tag="IV250_002557"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8769794.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(995..1270)
                     /locus_tag="IV250_002558"
     CDS             complement(995..1270)
                     /locus_tag="IV250_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HAP8769795.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(1242..2195)
                     /locus_tag="IV250_002559"
     CDS             complement(1242..2195)
                     /locus_tag="IV250_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP8769796.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            2807..4300
                     /locus_tag="IV250_002560"
     CDS             2807..4300
                     /locus_tag="IV250_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepR"
                     /protein_id="HAP8769797.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            4435..4731
                     /locus_tag="IV250_002561"
     CDS             4435..4731
                     /locus_tag="IV250_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAP8769798.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            complement(4755..5714)
                     /locus_tag="IV250_002562"
     CDS             complement(4755..5714)
                     /locus_tag="IV250_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8769799.1"
                     /translation="MTYTHLTTDELVMIESYFKINQSVAKTAHFLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIR
                     CSARTIYRMFKKGLFNPSDLPMKGKRKPNGHQERRGKQAFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIENKLNHWFESVPKNLFK
                     SITFDCGKEFSNWKQISNANDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDESFIQSVASKRNNIPRKSLNYRTPLEVFLSYVSIDDLSNLI"
     gene            5897..6748
                     /locus_tag="IV250_002563"
     CDS             5897..6748
                     /locus_tag="IV250_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769800.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            6769..6981
                     /locus_tag="IV250_002564"
     CDS             6769..6981
                     /locus_tag="IV250_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769801.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
BASE COUNT         2442 a         1199 c         1330 g         2426 t
ORIGIN      
        1 attgctttac ccatttgtca aatgctgaag ccgttagttc atattctcga atgatttcta
       61 cacgtggctt tccagctaag taaagattga cgatttgttg cttgaattct tgtgaaaaag
      121 ttcttcgtgt tctcttagac ataaaaattc ctcctggtat gttttcttct agtctacaca
      181 ccttaatttt tctgtctagt taattgtagc ctatccatga tcggtttatt agacctttga
      241 ggtctatatt ttttaactac aatcgacctt aaatttaatt gtttcattag cttttggaca
      301 tgtttaagag atactgaaat cccttctttt aataaaactt gatggatctt tgtggcacca
      361 tagcgttgtt tattcgtaaa aaaaattgtt gaaatcttct ttttcaactt attatttctg
      421 atttctgtag ctgtcaacgg tttattctta taaaaataat agacacttct agggatttct
      481 aataaccgac acaacaaaga aacagtgtaa tccttacacc atttttcaat aaattgaaca
      541 agcgaattta ggttttccga gagaatatgg ctgcggcttt ttttaagatt tcaagttcct
      601 catttaaacg agcattttct tttctcagtt cagctacttc ttttccagtc agtcctgtgg
      661 actgatcagg taaatataaa ttcttccatt tgtaaatcgt tgcttcagat aagccatatt
      721 ctttcgctaa ccctcgaaca gaacgtccag tttgattcag ttcaacaatc atttgtttaa
      781 aattttcttc atatcgggtc ataaaaattc atcctctcgt agataagttt aatttattct
      841 tgtctctacg aaaagctgtc taagatttga tactaacacc aaatgttgta tacccccaaa
      901 caatacacat attttgaagg attaggatta ataaagctaa aaatatagta agtatactaa
      961 atatatttac tatatctagt atacttacta tattttaatc ttttgatcgt ctattatata
     1021 taatccgttc ctcgtcgttt aaagtatcaa cataggcgtc cattgcaagt ttgactattt
     1081 catacatagg aatatctttt gcataactaa gatcccgaat ggtttttagt acgggaggat
     1141 ctacttgaac tggttttctt tctttcgcag tgtactttcg ttcccctaac tcgacattaa
     1201 tatcatcttt aattgaaata tcttcagcac caattttctt cttatctttg ttcgttggta
     1261 ataatgccat ttagtttaat ctcctttcca agaggtagga tatgattggc cattgagtca
     1321 gcatatttag gtgtataatc aaatccttca atatcaccat gttgttcata gtattcaatt
     1381 ctttcttcta attctgtaaa gatgtctgca aaaatagccc accatcttcg atctacataa
     1441 tcatttaatt ggataccatt ctcaccatac atttctaacc gatccgagcc tttaacaatg
     1501 cttttaaaaa tgtttgattc accatactta tcaattgttt tttgatagtt tgcgtgttga
     1561 gaggaacgtc gcactgagaa aagaacaggc aaaattccaa tgatttgaag atgagactct
     1621 tcaaaattcg tgacaatcgg aactaaaact ttattaataa aatcttctgt tccttccatt
     1681 gcgtaccgtt ttaattcttg cattgcaata atataatctg cagcagttaa gaatgcacga
     1741 accacattat ctgttgatgg tgggcagtcg ataaagatat aatcgaaatt ttttctcaat
     1801 tcttctaaag gtttaatgaa ggccaatgat ctcttacgac gatcaggata tttttcggac
     1861 aagtattcat taagttcttc agatccagga gagccagcca taatatataa atgtggactt
     1921 acttgagtaa tacctgaaac ccagtcccct tttttgattg ctgcagacat ggatcgatca
     1981 aagctagtaa tttgatacgt tgatccaatg ttggaagtag tatttctttg ttggtcataa
     2041 tcaattagca acacgtcttt ttcaaaaagt tgactagaaa caatcgctaa taaatcattg
     2101 tctgtagtct taccaatgcc gccttttcta cctgtaagca ttacaacttt tcctagttta
     2161 tctgatgttt taaatgcagt ccgtaagttt tccatagttc cctccaattt cctatactaa
     2221 atatatttag tatacttcat atacttaata tattcgatat atctaatata ctccaaattg
     2281 ataagaattc agactttata aggaatatag tggatatact aaatatattt gctatattca
     2341 gtatatcaga tatattcaaa atgtcaaaat atatttgtgc gttttgcgtt gacaatttag
     2401 agtgaaaaga ggtaaactta attctataga aaccacagat aattcactat ttacaaagta
     2461 gcaaaagcag tggtactctt taaacagaaa aattacataa aaaaaagaga cggaaataat
     2521 tgattttagc agaatcaatt atttctgaat cacatggtta ggacatgtgt tcacgctcgt
     2581 ctcaagaata tgtaagtagc taccaactac ttatattaaa agttgatcgg ataccttttc
     2641 cgattgcctt tattctacca aaaaaaggcg agcaactcaa cagaagaacg tttatttagt
     2701 gtacttaaat taaatattct taaacactga agagagaaca catactatgg ctgagattaa
     2761 agctaaactg tgttctcttt ttgtttgaga aaggacgtta caatcgatgt tgagcgaaat
     2821 ttattcagct attcttaaaa acggcttacg tacatacaaa tatcgaaaca gcaccatgaa
     2881 acctgcaggt catattgatt ccagtaggag tggtgctatc tttgcgtacc gtagcaagca
     2941 attaatgaat gttgggcgtg gtatggtcat tacttcggaa gaagctattt tagagaacga
     3001 aaagaaacta acgcattgga cacctaatac ctatcgcttt ggtacttatg cggatgatta
     3061 tcgaacagtg gttaaaggtc attctgagaa aaacttaagt caaatcaaca catttgtagt
     3121 tgatatcgat tcaaaagaga accatcaagg cgaaattatt ttagcctgtt tagatcaagt
     3181 gggctacatg ccaacgctaa ttttagaatc agatcatggt tatcaagttt attttgtact
     3241 aaagacacca ggctatgtca caaagaaaag caatttcaag gtgatcgatg tagcaaaaaa
     3301 aatttcaaaa acaattcgtc tacagttggc tgaaaaattg tccggagtgg atctaggatg
     3361 taaccacttc ggaattgctc gattcccaca agaaaataat attgttttta aagaaatgaa
     3421 ctatcagtat tcttttgctg actggattca atggtcgatg aagcttgaat cagatcaaaa
     3481 aacaaaagaa gatcgagaac gaaaactaat tgtctttcct gagaaaaaag aaattcgtca
     3541 agtagatgaa ccgtggttcg atatgttact tcataaagca aacatcgtcg gcggagaagg
     3601 acgtcttggt cgaaataatg tgattttcac gctttcttta gcttattact cttcaggtta
     3661 tgcattggat acttgcgaat acaatatgtt cacattcaac gaacggttac aggaaccgct
     3721 atctgaagca gaattagtaa agattgtacg tagtgcttat tctggtaatt atcaagcagc
     3781 aagcagagaa ttcattattg aattgtgcca gcaatgggtt tcaccaacct taaaaaataa
     3841 tgatctgttc attcaacgac gtggctggtg gaaatttaaa aagccacgag atcaaagaaa
     3901 atatagtcat aaaaatgaat ggaaagaaga tctattggct tatctaagcg aaaaaagtct
     3961 tgctagtgag ccttatttga acctgtcaaa aaaagaattg gcttcacaac taacgatgcc
     4021 caagcgaaca cttgataaga tcttaaatga gttacgagag gagaataaag tcttctacaa
     4081 ggtaacacga ggaagaaatg gcggaatgat cattgcttca gtcaaaacgt tgttcgcgag
     4141 ggttattcaa ctaagtaaag aaaagagact agattattca acaaggatca cagaggtatt
     4201 tggactcaac gagagcttag tagaagagac atttagacaa ttatcaaccc ccttgaaccc
     4261 tcttaaagaa gtgaatcttt ttgaagtaga tacaggttga aaaataaaag gtgcaaattg
     4321 ccttatacaa tatatctatg atacttctgt aggtgttgct ccctttgcct agctacgtgt
     4381 aggtggtata cttataactg cgtatgcagt cttatgtatc atggaggaag ttgtatgtcg
     4441 ttaaaaaatt atgtctattc acatcctgtt aatgtgttta tcattggtcg tttgggatta
     4501 tctgtggaag tgttctgtga gctttatggt tttaagcagg ggactttgtc ttcttgggtt
     4561 accagggaaa aaacagttgc ttctttgccg attgagtttc ttcatgggtt gtccttagct
     4621 tctggggaga ctatggatca ggtatatgat tgtttgtgtg tgttagagca ggagtatatt
     4681 gagtataaaa tagctaacga gttaagaaaa aggaagaagt atatccagta gtagttttta
     4741 tttactctta attgtcaaat taagttagac aaatcatcaa tacttacgta actcaaaaat
     4801 acttccaaag gtgttcgata attcagtgat tttctaggaa tattatttcg tttcgatgcg
     4861 acagattgaa taaaagattc atctactgaa ttgaaatcca tagatttcaa taaaccatct
     4921 ctacgtaaca atccgttaga attctcgttt aggcctcttt gagacggcgt tcctggatca
     4981 gcgaaataaa tggcaatatc attggcatta ctgatctgtt tccaatttga aaattccttt
     5041 ccacaatcaa aagtgatgga tttgaataga tttttcggta cagattcaaa ccaatgattt
     5101 aatttgtttt caatatcaat cgcttgtcta ccacacggtt tcaatgtgat gataactttt
     5161 gataatcgct caactaaggt aattacagca cttttatgtt tcaaacctac gatagtgtca
     5221 ccttcaaggt gaccaaactc atttgagaat tggctataat ctttttcacg ttcatgaata
     5281 gagcgacgga aagcttgttt tccacgtctt tcttgatgtc catttggttt acgcttgcct
     5341 ttcatcggta agtcagaagg attaaatagc ccctttttga acatacgata aatggtacga
     5401 gcagaacaac gaataggaaa cgctgcacga ccaacaatca catcgggtgt ccatccttga
     5461 acaacctttc tttgaatata ttctgattgt tcctcaggta aaacaagcgg gcgtctacca
     5521 cagtttgatt tgtttttctt atactgttga tagtactcta aggctgattt tccttgcttg
     5581 aagaacaggt atactttatg gatcgtttgt cttgaacgat tcaagaaatg cgcagttttc
     5641 gcaacagatt gatttatttt gaaataagac tctatcatta caagttcatc cgttgtaaga
     5701 tgggtatagg tcatttgtac tcactcctta taattctttg gacggaacta ttttgagtgt
     5761 agcacaaatg acttttttag ttgtctagct taattttaca atcggcgtac ttaaaaaatt
     5821 ggttcattga gcaacctatc gctgctaaag tagcaaagga aaacgttaaa ggaaagaatt
     5881 tagggaagaa atcgaaatga aatcggacga ctggattgaa cacacctggc ttcctctgaa
     5941 aagaaatagc aaacgaaaga aattttgggt attgcaatta tggccattag gaaaactttg
     6001 tagttacggg gcacgactta ttcttgattt aaccgtttac tcgattggag tatttggaac
     6061 ggcttattac ttgataaatg atcccaatag gattgcagca tcagacattt ttacagtgct
     6121 aatgacgttg atttttgtca tgctattgtc tatttttact caattagtca tgcaatttac
     6181 caggcaaaaa ttaacagcag gaaaactttt caaaagccta ttcgatttaa ttgttgtaaa
     6241 tgcaattcta gcgactttaa taaaacaaat tgaacaatct ggaagaacaa caaaaggagt
     6301 gaaggatttt ttattgacgt ttattcactt tgtaaatgga cacatgtatt tgtttgaagt
     6361 gggtattatt ctcttttttg tcgggtatgt atccacgaaa cttattaaac gttcaattga
     6421 aaaagaaatt gaaattgacc tgattccgac acttcttggt tatgagtaca atgttgattt
     6481 tggaagatat gacgaattga tcaatacgga tatcgttcac acgagtatcc atgaatctac
     6541 tgttaagggc atcagatcta ttttaaaaaa tggccaggaa atgaaagcaa tagatcgaag
     6601 aaaagagatc tacaattttt atgaagtcga agcaagccta cgatatggca tcggaaaatt
     6661 gtggttagat ggaagtaata ttcgtcaaaa caaaccatac attttttgga acagtagtga
     6721 tttagaactt ccaatgaaag ataaataatt atggagaatc gtttgaaaat gattcctcta
     6781 tacaggattg tgcttatagt tggcatggag ttaaaaacct taaagaagga gcagttattc
     6841 aaagtgaaag aacgtcataa tcagttacta tgtagtaaaa cgggtacgat gaacattgaa
     6901 taccgaacag aatatttaga attaacgaat caaactgatt ttttgttgat tcgaaagcaa
     6961 caagagcaag aaaaaaatta atggctactt atagccaatt gattgatgaa tgggaacaat
     7021 tcaaggaatc taaaattcca gaacgtctct aaaagccttt atttgtaaag agcataagga
     7081 aacgtgggaa cactttttaa aaaaagtgtt ccaataggct gtgcctatta cgtttctcaa
     7141 aaataaatgt gggaacaaaa tatttaaaaa taggatagtg aaaggatagt gaaggatagt
     7201 aaaaggaggg tagtaggata gcaaaaagga taggctcctt ttcgctatcc tttttaaatt
     7261 ggaggaatta atatggaagt aagaataaga aatgtggatg agaaaatcgt aagagaaatt
     7321 gatcgaagat gtgacgaacg gttctgttgc aaagttttaa atctactatc aaataaggta
     7381 gaataataga aaaagat
//
LOCUS       DACDIE010000096         7328 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.098, whole genome shotgun sequence.
ACCESSION   DACDIE010000096 DACDIE010000000
VERSION     DACDIE010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7328
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.098"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            83..940
                     /locus_tag="IV250_002565"
     CDS             83..940
                     /locus_tag="IV250_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP8769802.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            933..1835
                     /locus_tag="IV250_002566"
     CDS             933..1835
                     /locus_tag="IV250_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP8769803.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            1868..2218
                     /locus_tag="IV250_002567"
     CDS             1868..2218
                     /locus_tag="IV250_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP8769804.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            2252..3190
                     /locus_tag="IV250_002568"
     CDS             2252..3190
                     /locus_tag="IV250_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP8769805.1"
                     /translation="MRYYFVDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPD
                     DIFVGTEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLI
                     TCIDRKTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAAR
                     KTTENEELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQ
                     RQTEKMIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTG
                     GLPGKSSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            3268..4161
                     /locus_tag="IV250_002569"
     CDS             3268..4161
                     /locus_tag="IV250_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8769806.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            4199..4762
                     /gene="amaP"
                     /locus_tag="IV250_002570"
     CDS             4199..4762
                     /gene="amaP"
                     /locus_tag="IV250_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP8769807.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            4776..4964
                     /locus_tag="IV250_002571"
     CDS             4776..4964
                     /locus_tag="IV250_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP8769808.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            4977..5879
                     /locus_tag="IV250_002572"
     CDS             4977..5879
                     /locus_tag="IV250_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP8769809.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            5911..6153
                     /locus_tag="IV250_002573"
     CDS             5911..6153
                     /locus_tag="IV250_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP8769810.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(6409..6780)
                     /locus_tag="IV250_002574"
     CDS             complement(6409..6780)
                     /locus_tag="IV250_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769811.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         2686 a         1235 c         1450 g         1957 t
ORIGIN      
        1 atatattttg ctttaaaacc aaatgaggaa tattctagat gtagaacagg ttagaaaaag
       61 attagaaaaa ggagtgattt tgatggagaa gagatacggc gtagttcctg tggtaactgc
      121 ttcagacgaa aattatgctc cttacttgag cgtgatgatc gcaactgcat tagaaaattg
      181 caataaaaca agacgcataa aattttatgt gattgacgat ggtctatcag aatatagtaa
      241 agaaggattg gaagaaacgg taaataaata ttcaagtaat gcaagtatcc aattcctgac
      301 agtggaaaaa gatatctacg aagacttcct agtcagtgac catatcacaa ctaccgctta
      361 cttaagaatt tctttaccaa acttattagc taaagaggat tataagaaag tcctttactt
      421 agattcagat gtgttagtac tagacgatat cgtaaaacta tatgatgagc cgttaaatgg
      481 aaaaacaatc ggcgctatta tcgatccagg ccaagtgaaa gctttggaaa gattaggaat
      541 cgattccgat gacttgtact tcaattcagg agtgatggtg attgacattg atcaatggaa
      601 taaaaaagaa attacagaaa aaactatcca ttacctcagt gaaaatggcg atcgaatcat
      661 ctatcacgac caagatgctt tgaatgctgt actttatgaa gattgggaac aacttcatcc
      721 aaaatggaac atgcagactt cgctgatttt tgaacgtcat ccagcaccaa atgaaaaata
      781 tgaacgtcag tataaagaag gaaatgaaaa accttcgatc gttcacttta caggacatga
      841 taaaccttgg aatacgttga aagatcatcc gtataccaat ttatacttaa aaaaattagc
      901 acacagtact ctaacgaaag taggcgagat caatgaataa aaaagaaata gcagtagtcg
      961 caagttgtaa cacaaaattc gtaccacatt tagctgcatt atttgtctca gtattagata
     1021 attgcaaccc gtctaaattt gttcggttct atgtgattga cgatgatatc gattttgaaa
     1081 gtaaacagct tttacgcttc tcagtgaaaa acgcacgaat gaactcagat gtggaattct
     1141 tgaaaatcaa taaagaattc tttacaaatg tggtaatcag cgatcgtatt ccagaaacag
     1201 cttattatcg tattgcgatt ccggaattgt tccgcggcac agaagttgaa cgtatcttat
     1261 atatggattg cgacatgatc gctttacaag atatctctaa attatggcgt cttgacttcg
     1321 gtgattcaat cgtagcagct gttgaagacg ctgggttcca tcaacgactg gaaaaaatga
     1381 aaatttcagc taaatcaatg cgttatttca actcaggttt gatgctgatc aatgtgaaaa
     1441 aatggttaga tgaaaatatt acgcaaaaag tattagattt catcgaacat aatccagaaa
     1501 aattacgatt ccatgaccaa gatgcattaa atgctatctt acatgaccgt tggttaccac
     1561 ttcatccgcg ttggaatgca caaggctata tcatggcaaa agcaaaaaaa catccaacag
     1621 cagcaggtga acgtgaatac gaagaaactc gcaacaatcc ttacattatc catttttcag
     1681 gacatgtgaa accgtggagc aaggattttg aagggccaac gaagaaatat tacgaaaaat
     1741 atgcagggat gacggctttc cgttgtgtag ctaaattccc taaatatcca aaatatgcaa
     1801 aagttcaacc aaaaacagat gtagttgtta aataatagaa gacaacaact gaagaggggg
     1861 agtaggaatg gaaattacaa tgtctaaaaa tgcagtagag acgttaattg aaaaggttgg
     1921 ggaaaataca aaaatcgcac tcgcgcttat caatgattcc gatccatttc taagggacaa
     1981 aggtgctttt gcaaaaggca gcttttttca aatcattcct tttgtttcgg aatttggaga
     2041 atatgcgact aaaatcgagc atccattatt ggacatctat acatctaaat tagagcaaaa
     2101 ttatttcggt aaacgattga acatggactt caacaaacag cttgacagct tttcattaga
     2161 aaatgaaatc gcagtactcg attataatat caaattaaag aactgcttct tcagctaata
     2221 gtttcgagaa agtccaaaaa ggaggtatac attgagatac tactttgtgg atgaagaaaa
     2281 gaatgtaata gaaacagatg aaaaaaaata taattggctc gtacttgata gtgcaaagcc
     2341 cgaagagatt gagcaagcaa tcgaagaatt cgagcttcct gatgatattt tcgtaggaac
     2401 cgaatatccg gaagaagttt ctcggatgga gcgcctttat gagacgaaac tagagcatcc
     2461 attttctttg gtagtgatta atctagatca gtcagataat cgtatcgaaa aaaaattaac
     2521 gccaatatct tttgtacttt ccgataatct cttgattact tgtatcgata gaaaaaccga
     2581 atttgtggat cggctgctga caaagcatgg aaatcattta aactcgtttg aaaagatcat
     2641 cacgtataca ttattagata tttacaccca ttacgtgaaa gaactaagag agatgaaaaa
     2701 gcgaattgac gctttggata aagcagctcg taaaacaacg gaaaatgaag aactgtataa
     2761 acaggcagat cttgaaagag acatcgtata tatcgatcat accttaagag atcaacggga
     2821 aacaatggat caattatgga attctgaaac tttcatcaaa cgtttagacg atgaacagct
     2881 actctatgat gtccatttga gacagcgaca aacagaaaaa atgatcgaga tctatcgaga
     2941 tttattagag agtattggtg atctattcaa cgggatgatg gacaataatc taaatcatat
     3001 catgaaatat ctagattcag ctgcattaat catttccgtt ccggcgttgt tcgcagggat
     3061 ttggggaatg aatacaggag gattaccggg taaaagttct gctctaggct ttatactcgt
     3121 agtctttggc tctttgatag cagcagtagt tttaggttac catttatacc ggaaagatta
     3181 tacaaattaa agaatctatt taaaacaggt atcaaacaaa gaaatttgtt ttggcaatac
     3241 aatttaaaaa ctaggaggaa tacagcaatg acagaaccaa aattaatcga cccgcgaaac
     3301 ttgtaccata cggagaaatt tcctaaacaa gatcaggata caccagcttt acagcagaat
     3361 atgattcctg ttcctgattg tggagaagaa tcctatgaag gcaataatca actagaaaac
     3421 cgccgtgtac taatcactgg tggagattca gggatcggtc gtgcagcagc gattgcattt
     3481 gctagagaag gggcagatat cgctcttcaa ttcttcccag gagaagaaaa agatgcagag
     3541 gaagtagcaa aatacgtgaa agatgcagga cgtaagattg tcttactacc ttttgatctt
     3601 cgtgacgaac aagcacctaa agaaatcata gataaagccg tgagtgaact tggcgggcta
     3661 gatacgatgg tactaaacgc aggacagcaa atttcttgtc agtctattgc tgagttacca
     3721 cttgagcaag tcaaagacac atttatgata aacatcatcg ctatgtttgc tttagtgaaa
     3781 gaagctgttc cgcatatccc agcaggagga gcaatcgtaa caacaacttc tgttcaagca
     3841 ttcaatccaa gcgaacattt attagattac gcagcaacaa aagcttcaat cgctaacttt
     3901 acgatcggtt tagcaaaaca gcttgcacct aaaggaatcc gtgtaaatgg tgtagcacct
     3961 ggtccaatct ggacaccatt acaactagac gaaggacaac cttctgattc gatccctgaa
     4021 tttggtcagc aaagtttact agaacgtgca ggtcagccag gagaattagc accagtttat
     4081 gttttcttag catcgaataa agcaagttat gttacagccc aaatttacgg agtaactggt
     4141 ggagaagcga tcaacttata agaagttgat cgtttcaccc ctatgaggag gtgcgaaaat
     4201 gaataaaggt gtaaaaacga tcggagtgat cggaacatta cttttgctat ctgtgctttt
     4261 gtttgcgata ctattaagtg caacctatcc attgcctttt cagctagaga agtttcgatt
     4321 cttttccatc actaattcat acgtgcaaca atatgtattt tggcttgccg ttgcatttgc
     4381 tattttgaca attatcacgt tacttgtttt cctgttttat ccaaaaacaa tcggaaactt
     4441 tctactgaaa catggtgatg gggagttgac gttggataaa aaagcaattg aaggaatggt
     4501 tcgttctcac ttgcatgaag atgaattcat ccattcacca aaagtaaatg tcaaagcaac
     4561 gaaaaacaag atcgccgtca atgtaaaagg ggagttgaaa cgaacttcag cactcattgg
     4621 taaaacagga acattgatga aagaaatcga aaatgaagta caacacatat tgggaacaaa
     4681 agagccagtg aaagtatcgg tgaaatacac aggctttgaa gaaccagcta aacgaagcaa
     4741 tgaccgcaaa cgagtagaat agggggggac caaaaatgaa agaactattt tcgacataca
     4801 agctccctat catctttggg gtacttggat tgatattagc cgttttatgg atcaccgtgg
     4861 gctttttcaa gaccttattg atcttgatca tggcagcgat cggcgtcgct atcggttttt
     4921 atctgaaaca aacgaaaatc ttagatcact attttaaatc ttaggaggaa acatccatga
     4981 ataacaataa caaaaataat aacaataaaa tgaacaacca aaaaccagtg tctgcacatc
     5041 cagacccaac gacaccaaac caatcaaatg gtgtgaacga tgaaaagaac aaaacagcac
     5101 cagtatctgc acatccagat ccagtagtcc ctaacgttca acaaggagta gaagagaaga
     5161 aacatgatgc agaaaagcat gcacctcatc atccaggaac tccagaatct aaagttccag
     5221 aacaacatga tgcaatcgat ccttctaaac ctgtggaaga gcaaaaacat ccttctgacc
     5281 atgcgaaagc acaagaaaaa cttagttctg accacacaaa cggacaagga aaacttggtt
     5341 ctgatcatac aaataaccaa ggaaagcatg attctgaaca ggaagattca atgcgcggtg
     5401 agctgaatta tgaagataaa gttgtgcaaa aaatcatcgg aatcgcaatt gaacaagtag
     5461 acggtttatt atctgcaaac ggtggtttct tctctaatgt ggcaggaaaa cttgtgaata
     5521 cagataatgt aacggctggg atcgaaacag aagtcggtaa aaaacaagta gcagttgata
     5581 tggatgtcat tgtggaatat ggcaaagata tcgaaaaaat ctttgaagaa atgcaagaag
     5641 taatcggcaa agaagtaaaa aatatgacac atttagaagt gatcgaagtc aacgctaacg
     5701 tagtggacat caaaacaaaa gaagaatttg aaaaagatca agaaacggtg caagacaaag
     5761 taactgacgc agcgaaaaaa accggtgaat ttgcttcgaa tcaaacagat aaagtaaaat
     5821 cagcagctgg aagcggcgca caaaaagtaa aagaaaatac agaaccacgg gttcagtaat
     5881 aaggaagtaa aacattagga ggaacgaaac atgggattta tttggtcact tatcgtcgga
     5941 ggaattatcg gagcaattgc aggagcaatc acgaataaag gttcttcgat ggggattatt
     6001 gcaaatgtcg tagcaggttt agttggatca gcacttggtc aagcattatt aggttcttgg
     6061 ggaccaagta ttgcaggaat ggcaatcgtg ccatcattga ttggtgccat cattttagta
     6121 ctaatcgtat cattctttgc tcgaaaactg taaaaagact tttaaaaaga ggtcgtgaaa
     6181 aagctgttct tcatcaagta ataagaacag ccaaacaaaa atagcttctc atatttttca
     6241 tttggttggg catattatcg aagatgcaag cttttgaaca ctgtttatac ggaataagag
     6301 gctgtgacaa gacttttgtc acagcctctt atttatgcgg gcaaattccc aattagattt
     6361 ttaaaagttt ttccttaaat gtgtctttat tgatacagag aagcttcttt aataaaacaa
     6421 tgcctcccta aattttcttg agataggaaa ggtgaacata caaaataata cgaccacttt
     6481 gaaaacccag atgatccaca aatggatcgt atgatactga tgaagtaaaa agcaatggat
     6541 cagaaactca aagagattga aaaagacaag gaagaaataa tatctctttt ttactcttgt
     6601 ctgttttagc caataatagc tcgtaaagta atctagacaa acaaatagaa gtaaacaaag
     6661 tttcgtaaga gcagcaatcg tatcatgttg ctctgctaga cgattgacga gcaggaaaaa
     6721 tgttggtaat ggaatataga tcacgagtaa tgaaagaaaa aagtaaacaa taaatggcat
     6781 actattcctc ctaattgtgt gagttcgccc tcattttaaa ataacgatat ttgtttgtga
     6841 agctttttcg tgtcgtaaca aatgttatct aatgtatttt taaataaata atcattatta
     6901 tcaaaataat gttaaaaata aaaaagaatt gtccatcact gttattcaaa tcattttatc
     6961 aaaactacat agtaaatgtt aaaaagcaac atgacataat atatagaagg ctttacagta
     7021 tatatgggag cttactaaaa aagaatggag taagagttaa cacaatgggc cattagctga
     7081 agttttaagc acttcaaggt atgaaacaga atggcctgta atactacatg attatttaga
     7141 ctgctcaaac tgctatcagt ctggtggaat gaataaagta ttatactaaa agcagatcca
     7201 actacaacgt aacaggaaac cagttgtatc aggaaaagtg atagtacatg gagaagaaaa
     7261 ggcccccttt gtatgagtca gtacatatct tatttataga taaaacggta ttcaatcgaa
     7321 ataaaaaa
//
LOCUS       DACDIE010000097         7308 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.099, whole genome shotgun sequence.
ACCESSION   DACDIE010000097 DACDIE010000000
VERSION     DACDIE010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.099"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(1..826)
                     /locus_tag="IV250_002575"
                     /pseudo
     CDS             complement(1..826)
                     /locus_tag="IV250_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008378728.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            complement(909..2018)
                     /locus_tag="IV250_002576"
     CDS             complement(909..2018)
                     /locus_tag="IV250_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP8769812.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            complement(2312..3337)
                     /locus_tag="IV250_002577"
     CDS             complement(2312..3337)
                     /locus_tag="IV250_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8769813.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            complement(3582..4853)
                     /locus_tag="IV250_002578"
     CDS             complement(3582..4853)
                     /locus_tag="IV250_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HAP8769814.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYLAAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQ
                     LIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            complement(5137..6084)
                     /locus_tag="IV250_002579"
                     /pseudo
     CDS             complement(5137..6084)
                     /locus_tag="IV250_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634498.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
     gene            6096..6569
                     /locus_tag="IV250_002580"
     CDS             6096..6569
                     /locus_tag="IV250_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8769815.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            complement(6742..7296)
                     /locus_tag="IV250_002581"
     CDS             complement(6742..7296)
                     /locus_tag="IV250_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8769816.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
BASE COUNT         2282 a         1433 c         1084 g         2509 t
ORIGIN      
        1 acataacgat tgccgaacca tatcccttgt catttaaaat aaatgaatgt ttatagaata
       61 aataggctaa tgattgtgat ttaaacatgg cggtactatt aggagtaatc atcataaaaa
      121 taacatcaaa tacttggaaa gcattgataa ctgttgttac aactacaaaa aataatgttg
      181 gtgtaactaa tggtaacgta atgttgaaaa actgctggat tggactagca ccatcaattt
      241 ccgctgcttc ataataatct ttaggaattt cttataagcc ggctagtaat aacaccatgt
      301 tataaccaat agcactccaa actcctacaa tgataataga ataaatcgca atcgatggat
      361 ccgttaacca tgctggtcca tcgagaccta ttttagccaa catgttatta atcagtccat
      421 aatcattatt gaacatccag cgccaaatca ttgcgactgc ggatggtgca gcaacgactg
      481 gtagaaaata aattgtccga taaatcgaca atcctttaac ttttctattc attaaaactg
      541 ccaccaacaa ggataataca acaatactag gtatcgaaac cactgcataa attaatgtgt
      601 ttaataaaga ctgaaaaact tcaccgtctg caaacatttt ttggtaattt cttaatccga
      661 cccattcagt atcaccgaac cctaccgttt tttgaaaact taaaattgct gtttgaattg
      721 ctggccatat atttaataaa attaatccta atgccgttgg aaaaataaaa atatagcccc
      781 atatcacttg atctttacga tattttccaa aaattcgatt tttcataata tccccctttt
      841 tggtattcat ccattaatat acagacgaaa tagaatcctt tcgcctgtat attgtataat
      901 taactaattt aatttccgct caatatcgca ttgacttctt tttcaatttg agcggttcca
      961 tcttcaacag ttacttcacc agacagaata cggttaataa tttgaacttc tgcttgttca
     1021 gctcgtaatc catctttatt gtatggacga gggactccat actccaacat ttttccaaaa
     1081 gcactcaaat caattgtatc tgaagaagca gcccactcgt cttcaaagcc ttttagtgct
     1141 gggattgatg cccccgtttc cgcttggatt ttattggcgt cctcactagc taaaaatgcc
     1201 attagttttt tcacttcttc tgggtatttt gtactcgcac tggcagccca accaagtcca
     1261 tttgtaattg ttgcattaac ttttccctta ggtaacacgg cagaagcaac attttcaact
     1321 agatagtcat ttttcaaaaa tcccggtgca aaccagtttc cttcaatcat cattgctaat
     1381 ctacctgatt gaaaacgact taacttatca ggatcttgta attctgtaat tgttggtgaa
     1441 gcatgatcaa caagtgcaat atctcgataa aaagctaaag cttcttgtgc ttctggggta
     1501 ttcaaacctg attttgtatt gtcatcggca aatacttctc ccccattttg ataaataaat
     1561 ggccagtagc cagtctctgt tccaatatgt gatgcaaaac catagatgcc ttttgattca
     1621 tctgttaaat caattgcagc ttgtctgagt gtttcccatg tccatgtttc atctggataa
     1681 tcgacacctg cattatcaaa taattctttg ttgtaccata gccccactac agaagcatct
     1741 ttagggattg cgtatagttc gtcttcaagc gtatagaaat cagcaattcc ttcagtaaag
     1801 tttcccatgt caatatcatt ttctttgata acatcatcaa gtcccatcaa agcattctgt
     1861 gacatatatt tataggattc gctagagtgc atggttacta catctggcat attacctccc
     1921 gttgttgcag cttctaattt aacccaatat tgatcccaag gcgttgtctc catttctact
     1981 ttgatatcag gattttgttt ttcaaattca tctaacattt ttcttagtcc tggagcttgg
     2041 tcagtatctc aactcgcata gcttaaagtt acttgcccgt ctttagaatt actatctccc
     2101 ccaccacatg ctgcgagtaa tgtcccagtt aaaacagcta tccctgttaa agctattttt
     2161 ttgaaatgtt tcatcttaat tcctcctgtt ttgaatgcgt tttctttttg ttaacaacac
     2221 cataactgtt tacttaatgt tttgtcgaga ttttttacta aaaatattag taaacaaaaa
     2281 agtaaacacc cttttttgga tgtttacttt tttaatcgat atttttttca ctttctctaa
     2341 caataatatt attagaaaca atcactttga tcgaaacatt tctaacttct tttattcgtt
     2401 cattaaccat gcgaactgct agttttccaa tttcttctgt attcacattc accgtattta
     2461 atgcaggtgt taaaaattca gcaacttcaa tattatcaaa actcacaatc gaaatatctt
     2521 ctggaatatg tagaccattt ttttgaagtg aacgataaac ccccacagca ataggatcat
     2581 ttcctaccat gatagcagtc ggcaacttat ctttagcatg ttttttcaaa agtaaatctg
     2641 ctgcctgcat tccgtcaagt gtggtccagc cttctaaata aatttgacta tatttttcta
     2701 atcctttttg tttcatccac gtctcatagg ctatttttcg ataatcatca tctgacacgt
     2761 gtttatttcc ttggctatct aaacgaacac gtcctcctcc gataaacgca atgtttcggt
     2821 gtcctttttt atacaatcga tctaaatgct gattcgttgc ttttgttaaa tcagtataaa
     2881 ctgcatctac ttcagcttca atactagggt catcaatcac tacgacattc gaattaattg
     2941 ctgatacttc ttcaatcaca gaagatagca cctgcccaat aatcaacaca ccttgacatt
     3001 ttagcaaatc gtcttgctcc aacggtgttt ctgacaaacg aataattttc ttcaaattga
     3061 ctttgctttg ttctgcttcg acttgaatcc ctctgcgaat cgttcgaaaa tagggatctt
     3121 ccaactcatc tacttcagaa actgtcgtta ttaatgcaat atttgttaaa ttttttgcaa
     3181 cgggactttt tttgacataa tgataaaaat taaccgattc tgcaatcttt cgtttggttt
     3241 catcggttac tgaaagtgtc ggatcctcat ttagcacgcg cgatactgtt gcaatggaca
     3301 cacccgcccg ttttgcaaca tccctaattc ctaccataat agctcctctt ttcatatttg
     3361 aaagtgttta ctaacatata atcatacatt ttgaaaataa gcaaatttcg ttaaaaatag
     3421 gcactgtaat cataattaaa aacaagaaaa aatatccgaa caatttagag taaataatgc
     3481 taattctagt gaatttgcga cgaggaactg ctagatcaca gttcattctc tctatcgttc
     3541 ggatatttta ttgttaacta tacttatttc acagagcttt tttatttgat tttgtgaaca
     3601 gagtctcgag aaatcaattc tccctctaat agtagtactt gattttttat ttcttcttgt
     3661 ttggccagtc caatcagttg gtgaaaagca atcgccccct tgttacctgg atttagtcgt
     3721 attgttgtca acgcaggatc cattaattct gcaaaagtaa tatcatcaaa tccaataatc
     3781 gataattgtt ccatcactcg tttattctta ttttttaaaa attttactaa tttagccgct
     3841 aaataatctc cagtaacaac aattgcagta aattcagcta attcttgata aattcgctca
     3901 aattcagagt tgttatatat ccaatgcgat ttaaaggtta aatgatattt ctttaatgta
     3961 tcgatataag cttgatgtcg ttcttgaata acacttggtt cacctacaat gtagtcaaaa
     4021 gaaagaaacg ctattttctc atgaccataa tgaatcaaat gttccactgc caatgtacta
     4081 gcttggtaat catttgtatt tacaaataaa cgattcattt gttgttcaag ttctcttaat
     4141 gtctcttttg aaagatgcgt atcaatataa acaacaggac catcaaaaac gttatttaat
     4201 tccaaaaatt cttttacacg tacaccaaca acaatgaacc cctcaaaacg ccaatttctc
     4261 tgaatagttt taacatcttc tgctgaatta acattatgca ccaatacaaa ataaccatga
     4321 ttctttgcct gttcctcaat tccagacaaa acttctgcgg taaatggatc agaaaataca
     4381 aaatcagcat tatcaatagc cgaaaaataa agcaatccta ttaaaaatgc aatcgtctta
     4441 ccgattccct gacagacaga acacattcca ctaggatcat taaatgaata agcatttgca
     4501 tatcctaaac taggtgttgc aaaacatgaa aacaacacac gaaataacgc attgatgtta
     4561 gtaatcgtcc ctaacgttga gcgcgcactg ccaccaatct tcttttgatt gaccacaatc
     4621 gccgtagaaa gattatcaat ctcatcaacc tctggacgac tgtatttagg taaaaataaa
     4681 cgggtaaaac tatcataggt gtcattcagt tgacgaccag cttcttgggt aatagtatca
     4741 aaaacaatcg atgattttcc tgaaccagat acgctggtaa aaatactaat tttatttttg
     4801 ggaattttga gactgacatt tttaagttgt ttctcttgcg tggataattt caatatactc
     4861 gttcatttgg tactcccctt tattttgaaa tagttgagaa tgtttcaatg agacacggta
     4921 tttttggcag atgtttagtt tacctagaaa gaggtataaa aatcaagaaa aaaagagagg
     4981 ctgactcata agtagtttta ttagtcgaaa atgatagttt ataaaaatag gaaccgcatc
     5041 aaatcaacat tctttagaat ggatttgatg cgtttttctt tattttttga cttatgagtc
     5101 aacctcttac tcttcgtcat ggatttgatt agctaattat tgctccactt gttcacgaat
     5161 gaaatttaag tactgcggta aaaataaagg aatttcttca aagcgtggac tttgattttc
     5221 atacgctttt gcatctaggt ttccttcccc tgtagcaatt tcaatcaata attgatttgg
     5281 gtcacgataa taaagtgata caaaaaattc acgatctttt attcctgaat ttataaaatt
     5341 gcgctcttgt aattgtttgt cgatgcgttg taaatcagat ttattttcta cgccaaacgc
     5401 aacatgatgg acgctaccaa tcccttcaat ggcaactgga ttctcacgat cttgaataat
     5461 ctgaacttct tgatagaact gagtgtcttg attttctaag attgtcactc gttgatgacc
     5521 cgaaaagaaa ctgacgtatt ctttgtgcta ccagcctacc gacaataact ctcgttcagt
     5581 cgcatcggaa tattgtacgc gtagttgaat ggcatcaatt cctaaaatgg catgctctgt
     5641 agggatctgg tctgtgacat acgggaaata atcatcaata tcttcaaatt cgcgtaaggg
     5701 aactaaagct aatcgtgttg catctggccc ttcaaaacgt aagataggtc gtccagaaaa
     5761 tgattcgatt ccataatggc aaacgccttg tgtttcaaaa tatatttccc aaaaatgcaa
     5821 tgatgcttcg gaaggaactt ttagtatcgt tcgttcaatc gtattggttc caaaaaactg
     5881 gttgttccct tcttgtactt caaaaaaagt cagttcagtt cccacacggt ggtgattatc
     5941 tgaaaaaaac aggtgataca tggtatgatc gtcttgatta attgttttca ttagtaattt
     6001 taaaccgagt atattatgat aaaagtcaaa tgtcggtcga ataaagcgat tcacaataga
     6061 aatgtgatga atacgtgcgg gcataatatc taaaaatgag aatcaaacct ataaaagatg
     6121 gtgaacaact tttgttttca tcagaagaaa ataagcccct ttatttagac tatgtaaatc
     6181 acaatttgaa aatcatcatt aaagagaata acttaaaaca tatcacactt catggttttc
     6241 gtcataccca ttgtagtctg ctctttgaat ctggtgcgtc tgtcaaagaa gtgtatgttc
     6301 gacttggaca tacagatatt aaaaccacta tggatgatat atacacccat atcgcgaaaa
     6361 agcagacaga ggaaacggca attcgttttg cagatttcat gagttctaca gaggaaaagc
     6421 aaggaagttt gcagaaggtc aaaagtatgg tcaaaaacga aaaaagcaga ctatccctat
     6481 tagatagtct gctaaacgtt gagttatcag tatttttctt attccgcagc gtttgcgttt
     6541 ttgagaccgt attgattctc tttttttaat ttcatccttt tccaacctaa aaatgttatt
     6601 taattactta gtttatataa cacgaaccta taccaagtac cagtaacccc agcttttatt
     6661 ccgcgaactt tatgaaataa aatcgttcta gataaaaata gcttttaatc tttgtcttta
     6721 ctgtttataa aaatttttaa attatacacc tagtttaaat ccacgaagtg atgctcctat
     6781 ttcatcttca gttataccaa tgtacctttt tgtaattgct tgcgagctat gattaaaaat
     6841 aaacattaag gtagcaatgt ctctagtttt cttgtagtag tgatacccaa aagtttttct
     6901 taatgtatgt gttcctatat catctcgtcc taatttttct gctatatctg catatatctg
     6961 atagacacga tcccttgtta tatgtcgacc ttgttgccga ctcggaaata accaatcatt
     7021 ttcttcttta ccttcagtat ataataaaat ttcttctctc aaattatcta taaaaatcgg
     7081 ttttcgctta cctgttttat gttcaacaat aataggctta cttttgcctc gtatatctcc
     7141 aacttttaat cctataatat ctgacatacg taacccatta ttgattccaa acaaaaataa
     7201 aaaactatct ctttccggat tacttgttct tcttagccaa aactgaaaat cttgtatctc
     7261 ttcatttgtc cttaacggtt gtacattata gctcactatt gtttccct
//
LOCUS       DACDIE010000098         7223 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.100, whole genome shotgun sequence.
ACCESSION   DACDIE010000098 DACDIE010000000
VERSION     DACDIE010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7223
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.100"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(26..178)
                     /locus_tag="IV250_002582"
     CDS             complement(26..178)
                     /locus_tag="IV250_002582"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769817.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
     gene            complement(369..1247)
                     /locus_tag="IV250_002583"
     CDS             complement(369..1247)
                     /locus_tag="IV250_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8769818.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(1286..2404)
                     /locus_tag="IV250_002584"
     CDS             complement(1286..2404)
                     /locus_tag="IV250_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAP8769819.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(2401..4500)
                     /locus_tag="IV250_002585"
     CDS             complement(2401..4500)
                     /locus_tag="IV250_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAP8769820.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(4551..5372)
                     /locus_tag="IV250_002586"
     CDS             complement(4551..5372)
                     /locus_tag="IV250_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8769821.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(5369..6178)
                     /locus_tag="IV250_002587"
     CDS             complement(5369..6178)
                     /locus_tag="IV250_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8769822.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(6199..6693)
                     /locus_tag="IV250_002588"
     CDS             complement(6199..6693)
                     /locus_tag="IV250_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8769823.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(6705..7193)
                     /locus_tag="IV250_002589"
     CDS             complement(6705..7193)
                     /locus_tag="IV250_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8769824.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
BASE COUNT         2189 a         1673 c         1210 g         2151 t
ORIGIN      
        1 tattttatac ggctgaaaaa atctatcacc aaaaaaattc aaacattaag ccaaggatta
       61 tgaagtcaac atttcgaaaa atgaacatac attggtaagc aactataact attaccacag
      121 acataaagta acacaatata ttactattta agtgtacgcc accagtgaat atcctcaagg
      181 tatacagcgc acaacaaaaa gaaaaaagga aacgcagaag catttccttt cagtgattta
      241 atgaaaacaa cacgatttga cttagagcct ttttatttga attcattttc gctttatcca
      301 acttcatttg atcatttgat tactcggtta aatacttttg acatgagtat caatgaaaca
      361 ttcattaatt atggttgctt atttcttacc gcttttctgg ccaatgctaa tccgccgatt
      421 attccggcat tatcacctaa ttcgggcgta ataatgtagc tatcaattgg tggtatttca
      481 acatatccat ttataagctc aacaaattta tcctgaactt ttttcttcat gtgttcttgt
      541 ttcattacac caccgccaaa aataattcta tctggcgaaa acatcagtgt tgtattgtat
      601 gcacattggg caaggtaaaa ggcttcaatc tgccaaaacg aatcgtcagc caataaattc
      661 tggcctttta cacccaaccg tttttcaatc gctggtcctg ctgccatccc ctctaaacaa
      721 tcttggtgga agggacagtt tcctgaaaaa ttgtcctttg gatgtcgctt gatcaacata
      781 tgccccattt ccggatgact gaacccttcc acgaaagtcc cattgaacaa ggcaccacct
      841 ccgatacctg ttcccacagt gaagtatgca acacctgcta accctttgcc agaaccagca
      901 acatattcac catagcaagc cgcattgaca tctgttgtcc attcaatcgg aatattgaaa
      961 tattgcttta gcgttcctac aaaattgaaa ttttgccaag caagttttgg tgttgatgtg
     1021 atatgaccga aactcgttga tttgcattga atatcaattg gaccaaagca gccgaccccg
     1081 atgcctgcca attgttcctt aaaaggttta aaaaaatcaa tgacctgctt cattgtttca
     1141 actggagttg ttgtgggaaa actgactctt tccaaaatcg ttaattcctc agtaccgact
     1201 gcacagacaa atttagtccc tccagcttca attgccccat atatcatcat aggttcctcc
     1261 cttcgaatat gtgaaaaaca cttcattaac gcggcaagcg ttctcgatca gtgaaatgac
     1321 gtttagtatt tcctgaagct tctgttgaat accagtaagc gacactagca acatcgtctt
     1381 gccgctcaaa taagcgaaca tcatcgttgc cgatttgttg taaagtgacc gataaatctt
     1441 cggtaaaaca gatggggtct tgaagatgcc aacgataaag accatgtcgt ggcattgcgt
     1501 cgtcaccaaa ggcatgaacg gcattcaatt ttcccgtttc aaaacgatct cttgtggcat
     1561 ctctatttga ttggtaagga taccctaaaa aggctgttga atagtttttc gcatgtggac
     1621 gtccgaactc atcgtactca tgaaaggccc acgccccgcc aaaataatct tctgaacctg
     1681 ttgaggttac ggtcggatac tcactatccc catcaatgta gaatttaaac tcgccttcgc
     1741 cccaccaata acgttctagg caggtaagcg caaggtaagt acccacgtaa tagccttgac
     1801 cctttaatcg atcaattaac ttataatcct cctgtaattt ggtaatgcgt tcccgatgcc
     1861 aatttgcgtg aaaatatagg ctattttctt ccaattcgtc caccaaagcg taatttatcg
     1921 tataaaaaaa tgaggacata tccttcggat gtaaattctc gatcgtaatt ttagcggatg
     1981 tttggaaagg catttcgaaa taggaattaa agccccctgt aggattaacc actatcggca
     2041 acgagttcac ctcgcaacgc tctccaaacc cattacaaaa gaaatcccct aaaggacttt
     2101 caacagcagg gctatccgac ccatcccaat aaattcttaa gattaaatca cgtaaaacaa
     2161 aactgccaaa ttctgttcga tcacgaatcg tcatccacat atgacgaatt attcccgttc
     2221 cagtaatatc cgcaatcaca gtttcttcac cagaagtcag cggaattgcg ccacgacctt
     2281 tgcggcttgg tcctagctcg cttggcaaca tcgcagcttt acctttctcg cctgttggat
     2341 tttcgggagt gattgctcga ctctggattt ttttaaattg ggtaatattt tttagtatac
     2401 tcatcttttt ctcctttgaa tgtgaatcgt ttttcccttt tctacgtgaa gaattttttt
     2461 cgtatcactt attttcaatg gcttttctga caaatttgaa atactcaact cggattgcgt
     2521 acattcaatc gaaatgaagg cttgaccgaa cttcaaattc ttccaagaaa acgttaaatt
     2581 atcaacagga tcaatttgca atactccttt tttgaatttc aatccgaaat attgactgat
     2641 cattaggctg acaaaaagac ccgatgccca gccgcatttg ccgcagccaa agtctcgcac
     2701 gacttctcct tgttgagcct ctaagcgata cggccaccac caccacgaac cgtctaaatc
     2761 tgccaaacaa attagttctt ctaatcgggt tacgaaagct tgagggttgt gcagctcgct
     2821 cattaaaaca gtgataaagc caggaaaagt ggcgccggat tctaatcccc aagtgatgcc
     2881 tttgatttcc tcgctatagg ttggattgaa agaactagca gaaaaggcca tggtgttctg
     2941 atataaatga tctttattat ctaaaaattg ataaaaaggc atcaacgtag tgtctgattc
     3001 ttctccatca tgcatcaata aattaatttt tccatccaca atcacatcac ttaaaaagat
     3061 taacccttgc tcaagaatcg gtttttgata gtgcttcact gaataggttg ttttctcctc
     3121 atcaccgatt ccctccaaaa attgctcacc aaaaggacca tcgatagtca tttgtttaca
     3181 aattgattcc tttagtttac aggcaatgtt tttgtatcgc ttgctaagac tcagttgttc
     3241 tagggcttca aaaatttctg caagtccaat acaggccttc caaagacaaa gattgctgcc
     3301 ggtatgatac tttccaagtg caaaggcgtc tgataaccaa acagatggaa aaagcattgg
     3361 ttcccctgac cgacgttgag ctaatatgtc ctcaataatt cttgaagcat tcagtagaac
     3421 ctgcggacac cgctcaaaga attttgtatc cccagtatgt tcaaaataaa gactagccag
     3481 taacgcactg gataacgaat tactcagaga atgattgata cctcctgtaa attttgaacc
     3541 aggaaactta acgccatatt gatcaaacca tagaaccgtt ttttgacata actccggttc
     3601 aaacatggtg aatggcaatg atgcgtaata catatcctta ttccaaattc gattagtagc
     3661 aggatagctg ccccaattgc tgccgacgac ctgtccctgt cgattcatac cgaaactaga
     3721 aaagctttga taaagtgcac gacgatagag atggacaact ctatcgtttg gcattgacag
     3781 ctgcccaaat agctgagcaa aatagttcaa tgtttccttc agccattttt cgatatcaga
     3841 ttcctgaaaa gtcattactt cttcaaaagc atttgggtcc acaaaagcaa tggcgaagct
     3901 tgccacttcc tgttgtgcga tcgtgtcctt atttcctttt tgagaaatca acacattttg
     3961 ctgatccgaa tacttctctt gatacgaagg cacatctttc actgaaactt gaaggctttc
     4021 gtgtgtttca ttcacaatgt aaacctgttg aacaagcata cttaaacgct tattagccag
     4081 tatgggagca aaggatacta caatgacctt aaacgtcgaa taatgatgaa tatatctagg
     4141 caaacagtct tgaatcacat cgacttcaat acgatgatcc gattctgaca aatggtagac
     4201 aatcccgttt acttcaacct ttaaggataa cgaattggtt tgagagagct gtttattgac
     4261 ccaaacacct ggtttttcct cagttagtaa attttctcga taaaaggtca acgtttgaat
     4321 cgtcccgcaa acatcaaggt ttgctaatgt cttttgatta cttacatcga aggtcaatcc
     4381 ctttacatca ggaatattcc gatagtagct gtattcgttt gaagtagtca catttccgtc
     4441 gtttaactca gcattaaaaa atgggtgttt gggtgaataa attacttcat taatagtcat
     4501 cttttccctc cttgctaaaa aaactgcagc ctagtagctg cagcttccta ctatgcgatt
     4561 ccaagtaatg acaataaaat tcccaagacg accattccaa aaataatttt gatgacgccg
     4621 acttttttat ctagtaattt gaaacagctc aatgttacca ataacggtac aattccttta
     4681 aaaatactat cgaggatttc ttggacattc attagttctt cgcccttcat tgaaaagctc
     4741 aaaacggtat tgaatttgac catactgatc gtcattgcac ctaccatgat cagacccaag
     4801 atactagcag cttttgtcag aatttcaatt aacccgttcg catacagctt ttcaatatat
     4861 ttagagccta atgaatagcc taaaacgccg ccatagtatc gcatcaagta attaggtata
     4921 ttgaacataa ctaaaaagat aattggtgcc aaaatatttc cggcattggc taagttgatc
     4981 gctagtcccg cggtaacgac acgccaaact ccccagaaca atgaatcgcc aattccagcc
     5041 aatggtccca tcaaactcgt tttgatcgcg ttgatacttt ccacgtcaaa accagctttt
     5101 tcagaatttt ccttctccat cgatgccgca atccccatga caaaggacgc gcaaggaatg
     5161 gtcgtcgaaa aatactgtgt atgacgttcc agcgccgcag ctcgatcttc ttcacttttg
     5221 tagaaatgat taatgaaggg catcatagaa taggcgaaac caccgccgcc catcgtcacc
     5281 ggattcaccg aagcatacat agtcatcgag cgccaaaaca tcttccgaac caattttttc
     5341 tcatctttag aaattccatc agctaatttc attgaaagaa atcctcctct tcttggtcaa
     5401 gcgttgcatt tacagcagca tcgctttgaa ctgcatgaac agatatattt tttagttgta
     5461 gatctctttg ggctataaca attgcaataa tcgttccaac tgcggcaatt gcaatcaatg
     5521 gtaactcgag atacgccata acaacggccc caaagaaaaa ccacacagca atatttttac
     5581 tccaaagcat ccgtaacagc atcgccatcc caactgccgg cagcagattt ccagcgactg
     5641 ttaatccgtt catgatgacc tctggaatat tatccaagac gcttttgaca acatctgctc
     5701 caaataaaac ggcaaagaat ggaataagag cataaagcgc atagtggaca aaccacataa
     5761 agaagtgcag attgatgatt cctttttgat caccagattt tgcaaatttg aaaaatttag
     5821 cagccatcgg tccacataca aaagaaaaaa tcatattcat taactgtaat cctaatactg
     5881 ctagtggaaa gacaatggga ataatcgcat ccacgtttcc tcccattttg attgcgaacg
     5941 ctgtagccag cccagcagcc aaagaaggct ccgccgtact agagattccg atattgacga
     6001 cacccataaa gatggcttct agtgacgccc cgacaatcaa ccccgtctgc aaatccccca
     6061 tcaacgctcc tgctaacgga gcaacaacaa tcggacgact cagcatggac atcccgaaca
     6121 tttcatgtcc agcaactcca ataaaaacgg ccaaagcgac tacaaatgca tcatacataa
     6181 tttttctcct tcctttttct aaaaatcact caaaatagag atagctgaac gtttttccat
     6241 cgccggtgta ggctgaaaaa cggtatctgg ataaagttca actaattctt ttaagtcttg
     6301 aatttctgac gtcgttaaca tcacttcctt tgataaggtt ttcttatctt ccgatggcgg
     6361 tgtcattttc ccagcattcc ccacgttcat taattcgatt tccgttggga aagctttcgc
     6421 aacttttacc gcatctttga cattagggac taaaaccatc aagcgcatgt ccttcgcccg
     6481 cggatctttt aacaaatgaa tcgcgtcatc aactgtttta ataatgactt tgacattcga
     6541 tggtgcagcc atcttcaatg tcatcttttg aatatcgttg gtagccgcct catcgcttgc
     6601 tactactaac ccggttaaat ttagactctt tgtccaagtc atcgctactt gtccatgaat
     6661 taatcgttca tcaattcgca ctgctttaat catttttctt cctcctaaaa aaaatcttcc
     6721 tcttcattta ctacatgtac ctcatcaaga atggtcatct ttaattcctg ttggcattta
     6781 tttaccagtt cctgaatatc ctcctttgtt aattccttgg ttgccaatac tatttcgagc
     6841 agaattggca gattaaagcc agacaaaata gttacagcaa tctctttctc caaaaaggtt
     6901 ttggtaatct tctgattgac acttccgcca aataaatcgg taaaaacaaa aatctgatcc
     6961 tgctcgttca cctggtttac aaatacattc aactcctgtt caaaatcaca ctcatccaca
     7021 taagcattaa tcaccgtaat ctcttgaacc ttccccgtca gtaattgaat actgctaaga
     7081 aagcctgaag ctaagtgacc gtgagtcgct attagaattt tcttcatatg gttaattcct
     7141 ttctttctgt tcacttttat ataagcaatt cctgtgccaa cttttaaaaa cattggttta
     7201 atcacgttaa ttcacattaa aaa
//
LOCUS       DACDIE010000099         7215 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.101, whole genome shotgun sequence.
ACCESSION   DACDIE010000099 DACDIE010000000
VERSION     DACDIE010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.101"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(575..1591)
                     /gene="tsaD"
                     /locus_tag="IV250_002590"
     CDS             complement(575..1591)
                     /gene="tsaD"
                     /locus_tag="IV250_002590"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAP8769825.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(1588..2052)
                     /gene="rimI"
                     /locus_tag="IV250_002591"
     CDS             complement(1588..2052)
                     /gene="rimI"
                     /locus_tag="IV250_002591"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP8769826.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2059..2601)
                     /gene="rimI"
                     /locus_tag="IV250_002592"
     CDS             complement(2059..2601)
                     /gene="rimI"
                     /locus_tag="IV250_002592"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP8769827.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(2585..3409)
                     /gene="tsaB"
                     /locus_tag="IV250_002593"
     CDS             complement(2585..3409)
                     /gene="tsaB"
                     /locus_tag="IV250_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAP8769828.1"
                     /translation="MYYCTSFAFGIFILRIKKYATMEGENVIAERIKYMITLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVDNIIVPMFDARRKNVYAGAY
                     RWKENRLETVLEDVHISASDLFTQLQKLNEAIYFVGSDCHKFFSEIKEILPSAMINTV
                     PAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKRVEAEEKWLETHTPGDESYVEKI"
     gene            complement(3498..4478)
                     /locus_tag="IV250_002594"
     CDS             complement(3498..4478)
                     /locus_tag="IV250_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP8769829.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(4502..5986)
                     /gene="galT"
                     /locus_tag="IV250_002595"
     CDS             complement(4502..5986)
                     /gene="galT"
                     /locus_tag="IV250_002595"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAP8769830.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(5998..6987)
                     /gene="galE"
                     /locus_tag="IV250_002596"
     CDS             complement(5998..6987)
                     /gene="galE"
                     /locus_tag="IV250_002596"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAP8769831.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
BASE COUNT         1977 a         1568 c         1271 g         2399 t
ORIGIN      
        1 tcagggtcac tgcttatttt cctccctaaa acttctgaaa gatccggcag ttttagatca
       61 ctggacaaga ctcaaagatt ttctatgtgt gtttcagtta tgtaatattc actgtttgca
      121 aaaaatactg ttagattaac tagatgaagc gtcttgtcct catccgatct agattattcg
      181 atattcctcg acaataaacg attactcagt gagggcaatc agaggaacgg ataatgataa
      241 ataagctgta aaaaatagca aaataacaat aatgattaaa agtatgtaaa aaatagggct
      301 ttgacaaaaa cgtgtcacag ccctatttcg attaaacggt gtgtacctgt caactcttcg
      361 gtcacaagtc ataatgttca taaacatgag gagctgtttt ttgaaattgt tccttgtgaa
      421 tcagagttaa acgacaggct tacgaactct tgattaaacg gcgttcaaaa gcttgcatac
      481 gcggtaataa gaacagccag gtcaaaaaca tgagaagccg tttttcctgg gctgttctta
      541 ttacttgatg cagggcagct ttttcacaac ctctttattc agataaaacc aagcttggat
      601 cagcattcaa ctgatagcta gcaaaatttc ttttttgcat ttccacatgt gctgctgctc
      661 caatcattgc agcattatct ccacaaagtc gaagaggggg aatcactagt tctacctcag
      721 ggagtttagt tgccaaagca tcctgcaatc cttcacgcaa cccttgattg gccgctacac
      781 ctccagctat aatcagttgc ttgactggat aattttgaca agctcgaagt gttttagtaa
      841 tcaatacatc gatcacactt gcttgaaagc tagctgctaa atcatttttg tctagatctt
      901 ctccacgctg ttgtgcatta tgcaccaaat tgataaatgc actttttaat ccgctaaaac
      961 tgaaatcata attatcttca tggatcatcg ctcgcggaaa atggtaatta tcttttccct
     1021 gatgagctag ctgatcgatt tcttttccgc ttgggtaaga aagacctaat actcttccaa
     1081 ctttgtcata agcttctcct gctgcgtcat ctcttgtctc cccaatgatt tcatatgatc
     1141 catcttcttg catatagact aattctgtat gtcctccgct aacaagaagt gccatcaatg
     1201 gaaactggaa tggtttaacc agacgggctg catagatatg ccctgccata tggtttacag
     1261 gaataagcgg caattggtgt gcccaagcaa aagccttagc tgcagaaata ccaatcagca
     1321 acgagccgac aagtcctggt ccataagtga cagcgactgc cgataaatct tctgccgaca
     1381 caccagcttc taccagtgca tcttccaagc ataaggtgat ttgttctacg tgatggcggc
     1441 tagctacttc aggtactacg ccaccaaaac gtttatgact gtttatttga gaagcaacga
     1501 tgttagataa gatctctgtt ccatttctaa cgaccgctac actcgtttca tcacaacttg
     1561 attcaatcgc cagaatcaat tcttcattca tttcatcgtc tccgttttta actttctgct
     1621 cataatgatc gcatcttccg tcggattgcg ataatatgat ttccgtttac ctaatatacg
     1681 aaacttttct gattcataaa gttttcttgc tgcttcatta gatagacgta cttctaaaaa
     1741 aacatgcagc gaatctttta tttgttccgt aatcagtaat tgtaacaaat gccgtgcatg
     1801 cccttttcct tgttctgaaa ctgcaacagc aatatttgtg atctcgactt catctattac
     1861 tttcgagtat ccaataaagc cacagatctg gttatcttca gctaaaagca gataatcaga
     1921 gtgaggctgt tggatgtctg ccaaaaactg cattttcgtc caaggtgaac catgtggata
     1981 agcattttcg ctaatcatcc atacgcattc tgccaagtac gcttcatcaa aatctttttt
     2041 tctgcaaatc aactaatctc actcattttc taaaagtttt atcatatcca acgcatcttc
     2101 ttttgtttca gtgtaatatc tttttttgat cgttgttgct tgaaacccca atttccgata
     2161 aagtctttgt gcatcttgat tactcatacg cacttctaat gacatcgtct cacagcgatt
     2221 cattatagca aagttctcga tttcgtcaat aagcagcgaa ccgattcgtt tacgctgata
     2281 tgccggtcga acggcaatat tcgtgatatg gcaatctaaa ccgaacgctc ttgaaccgat
     2341 aaaaccaatc attcttccat tatcaaagat acctagatat agatgaggaa gcggtgactt
     2401 caattctgat ataaaggcac tttttgtcca tggcaattcg ccattataga cttctcgttc
     2461 gacatccagc agctctttga tatcttctgt agtcaattct cttagtacgt aacgacgatc
     2521 gatttgttcg actgtttttt ctgtataaat cattttatct tgaaaaaagc ttctggcata
     2581 cgctctaaat tttttcaaca taactttcgt ctccaggtgt gtgcgtctcc aaccattttt
     2641 cttctgcttc tactcgtttt aaatagcgag gaagaaaagc ttgtttctct gcaactggtt
     2701 caactaaagc acctaattga gccacagtta caccacttgg aatatcccat gctggaactg
     2761 tattaatcat cgcactaggc aatatttcct ttatttcaga gaagaatttg tgacaatcac
     2821 tcccgacgaa ataaatagct tcattcagct tttgtagttg tgtgaacaaa tcagaagcag
     2881 aaatatgtac atcttcaagt acagtttcta atctattttc tttccagcgg tatgccccag
     2941 cgtatacatt ttttcttcga gcatcaaaca tgggcacaat aatattatcc acaccgatgc
     3001 aattagccgc gatggttttc agactagata ctcctactag ctctttatct aatgtataag
     3061 caatcgtttt tgctgtcgtt acacctatac gaagccctgt ataagaaccg ggaccatcag
     3121 aaaccgcgat tcggtcaatg tcttttggac ttatttgaag atctgcaaac aattggtcga
     3181 ttgcaggcat cagtgttaca ctgtggttgc gtttgatatt tgtttgtatt tgtccaagaa
     3241 tttgttcgtc ttctaccaca ccgatcgcca aagtttggtt tgcagtatct attcctaatg
     3301 taatcatata tttgatcctt tctgctatga cattctcgcc ttccattgta gcatactttt
     3361 ttattctgag gatgaaaata ccaaaggcaa aactagtaca ataatacatg attatctgct
     3421 ggcgggcaaa ttttgcccct tcctgaaaca caaaaaagct gcggtatcac tccattttcc
     3481 cacagctttt ccatctttta tttcgttgat tctcgaataa tcaactctgt ttccacaata
     3541 atctttcttg aaaccggtgc tttggaatta cttaattgaa gaatcgtctc cactgctagc
     3601 tctcccatcc attcggtata aacttttaca gtagaaagcg agggcgttac atatttcgct
     3661 acactgacat cattgaagcc catcacagaa atatcttctg gtaccactaa acctttttct
     3721 tgaatcgctt tcagtgcgcc aatcgctaat gcatcattag ctgcaaaaag cgcatcagga
     3781 acatgcggat tcgtttttaa atactgcttc actgcttgat atcctgactc gacagaaaaa
     3841 tctgcttcga taattggacc ttcgtcgtag cctttttctg ccaaaacttg ttgaaatgcc
     3901 ataattctgg gatcttttag ccgctgatgg tttttcttcg tatattctgt tccagcaagc
     3961 atagcgatat gttgcgcttt atgggagaca aagtgttcaa tgactttttg aacacttgac
     4021 ttaaagtcga caactagaga gtcgattcct tgagaagtgc cgtcagaatc taccaaaagc
     4081 aaggcatttt gggatccttt catttctaat aactgagctt cgtcgaattt tcctagtgcg
     4141 atcgttccat ctgccggaag atcactaatg gaatcccacg tttctttcaa taaagtaacg
     4201 tttgcttctt ctgccttttt ttcgattcct aggcgaatag ataaataata aagatcttca
     4261 agctcttccg tatcgtcgta ccactggatc aggcgaagcg tttgcttagt atttgctgca
     4321 tttttacgat gtttcgtata atttaagcgt tctgctgctt caaaaatttt ttgctttgtg
     4381 gctaagccaa cggaaagatc aggatcatag ttgagaactc ttgaaacagt agcaggagaa
     4441 actcctgcca actgcgcaat gtctttaatt gttgccattt gtctctcctc cttttttacg
     4501 tttataaaat cgcagtaaat cgttcaaacg ctgcctgtcc tttttcatct cgcttgaata
     4561 ctccagcgtc ttccagtaca cgtgcaaaaa cctgacctac agatttttgt acgacatcgg
     4621 tgaccgtatc tttcgtcaaa tcaggatgtt ctttttttaa ttgttctgcc cacggacgat
     4681 gatattcagc catctggttt tcttctccta ataagaaatg ctctacttct actagttctg
     4741 gtttcaaacg aggtggtaaa acagcaagcc ccattacttc gatcaaccca atgttctcct
     4801 ttttgatatg ttggacatct ttatgtggat gaaaaatccc atcaggatgt tcttctgaaa
     4861 cattgttgtc tcgaaggaca aggtccaact caaagtaatt gtcccttctt cgtgcaattg
     4921 gcgtaatcgt atggtgtggt gtaccatctg ctgaaaaagc tctgatagat actgtttcat
     4981 ccgaatatgc tttccatttt tccaatactg agttagctgc ttcaataagc aagtttttct
     5041 ctgttgattg caaacggatc acagacatcg gccatttcac gactcctgcg atgacttgcg
     5101 gataatcttc tagtttgata agtttttcta tcggtgcttt tgccatagga aattcatgac
     5161 gtcctgcctg ataatgatca tgagaaagaa tcgatcctcc aacaattggt aagtcagcat
     5221 tcgaaccaac aaagtaatga ggcaagactt cagtaatccg aagcagtcta gcaaatgttt
     5281 cctttgtgat tttcatcggg cggtgttgtt ctgataaaat gatcgaatgt tcattgtaat
     5341 aagcataagg ggaatattgg aatccccagc tttcaccgtc tagattcatt cgaataatcc
     5401 ggtgattggt gcgggctgga tagttcaagc gacctctata tccttcgttt tccatgcaaa
     5461 gcatgcaggt aggatagtct acttttaccg cctcgcgttc tgctgcgatt tgttttggat
     5521 ctttttctgg cttagataaa ttgattgtga tttccagttc accatactcc gactctgccg
     5581 gaaatttgat gttttttgcg atggctcttg ttttgatgta gtcatttttc ttacttaaat
     5641 taaagaaata ctcagttgct tcctctgggc ttttcgaata gtgctgtgca aagaatgcat
     5701 tgacgactga aggtggcggt gtgagaaaat ccattaattg cgcttcaaaa atctcttgtt
     5761 ctgctggcaa ttctgaaaga acaccatttt ctcttgcctt agcgacaagc tgatccaata
     5821 agtctacaga ttcttcctta actggtctta cttcaatgct ttctagagca tcttctccta
     5881 ccatccctaa aagacgattc tgtaaatata agcggtccat ttccatccat ccgccagctt
     5941 cgatcgcaag tgtggcaaaa tcacaaatcg tttgactgat tgacatatct cattccccta
     6001 ttcttcataa ccatttggat ggctgacatg ccaatcccat gctgttttga taatcgcttt
     6061 gatatctgtg tattcaggtt tccagcccag aatctctctc gctttttcac ttgaagcgac
     6121 taatcggcta ggatctccag ctcttcttgg tgctatttct gctggaattt cttttcctgt
     6181 cacttctcta gctgcttcta gcatttcctt gactgagtat cctttgctgc tgcctaaatt
     6241 gaagaagttg ctttcatttc cttctttcaa gtattccaaa gctaaaatat gtgcagcaat
     6301 caaatcttct acttgaacat aatctctgat acatgttcca tctggtgtat cataatcgtc
     6361 tccataaaca gccaaggctt ttctttgacc aagagctact tgtaaaatga ttgggacaag
     6421 atgcgtttct ggtgtatgat cttctccgat agatgcatct gcttttgctc cggctacatt
     6481 gaagtagcgc aaagcgacat agcgcatgcc atatgcttgg tcacaccact tcatcatttt
     6541 ttccatcatc aatttacttt caccatacgg atttttagga ttagctggtg tatcttctgt
     6601 gattggagac tctttcggct ctccataagt tgctgcagta gaagagaata cgattttatt
     6661 cacgttgaac tcgtgcatga cttcaagtaa gatttgcata ccgtatacgt tgttgttgaa
     6721 atacatcaat ggtttttcga cagattctcc tactaaagag cttgcagcaa aatggattac
     6781 tccttcaatc ggttcctttt caaatacgga acgtagaaat tctttatccc ggatgtctcc
     6841 ttcataaaaa tgagcatctg gatgtactgc ttgtttgtga cctgtgagca aattatctac
     6901 cacagctact tgatacccct tttgaactag ctggtctaca gcgtgcgaac cgatataacc
     6961 ggctccgcct agtactaaaa ttgtcataaa aaacactcct ttgattagta aaattgtgtt
     7021 tgttgattct atataattta aaaaacgaag tttactttta tggtgaaaac aaacaaagta
     7081 aaatttcgta aattcatatg gtaaatttat aataaaacat ttagtaaaca ttttcaagga
     7141 ttttttgcaa aggtttacaa acaaacgtaa aactagcctt tttttctctt ttgttccata
     7201 atagatataa caaat
//
LOCUS       DACDIE010000100         6770 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.103, whole genome shotgun sequence.
ACCESSION   DACDIE010000100 DACDIE010000000
VERSION     DACDIE010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6770
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.103"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1503
                     /locus_tag="IV250_002597"
     CDS             <1..1503
                     /locus_tag="IV250_002597"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAP8769832.1"
                     /translation="LIFYKYLSDRLLEQVVLLADESLEEYDTVSKQTMLYRELLSDEE
                     SKEDLIATIVDILGYAIAPEYLFNVLADQAKQATFQLNDLNKAFVQLASTYNQFNGLF
                     DDVDLQSKKLGTDEQQRNVTITEVIKKLNDVDVLEHDGDVIGDAYEFLISQFASEAGK
                     KAGEFYTPHMVSDMMAQIVTLDQKERRFFSVFDPTMGSGSLMLNVRNYLTHPDNVKYH
                     GQELNTTTYNLAKMNLILHGVDAEEMNLRNGDTLNKDWPTDEPYTFDAVVMNPPYSAN
                     WSADTTFLDDSRFNRYGKLAPKSKADFAFLLHGFYHLKETGTMAIVLPHGVLFRGAAE
                     GVIRQKLLEDGSIYAVIGMPANLFFGTSIPTTVIVLKKNRQTRDVLFIDASREFVKGK
                     NQNKLSEENIQKILETYAERKDVEKYAHLATFDEIKENDYNLNIPRYVDTFEEEEPID
                     MVHVGNDIKKIRQEQQVLEKKLLEAISSLQTTPENEAWLQGALEVFKHEQ"
     gene            1493..2710
                     /locus_tag="IV250_002598"
     CDS             1493..2710
                     /locus_tag="IV250_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP8769833.1"
                     /translation="MSNDTQPEIRFPGFTDDWEQRKLGDFVVDYVEKTSVQNQFPMLT
                     SSQQKGIVLQEDYFANRQVTTENNIGYFVLPRGYFTFRSRSDNDVFVFNRNDIIDRGI
                     ISYFYPVFTLKSADSDFFLRRINNGIQRQLSIQAEGTGQHVLSLKKFKNIVAMFPSEE
                     EQQKIGTFFKQLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWE
                     ERKLGELGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIER
                     KKWKPIKDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFE
                     TINLPRYSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLL
                     KETKKSFLQKMFV"
     gene            2723..5872
                     /locus_tag="IV250_002599"
     CDS             2723..5872
                     /locus_tag="IV250_002599"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HAP8769834.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLAIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PDKTKGLIVTFRKPATMEKNVEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYLPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
BASE COUNT         2361 a         1109 c         1377 g         1923 t
ORIGIN      
        1 ctgattttct ataaatattt atccgatcga ttgttggaac aagtggttct tttagcagat
       61 gaatccttag aggaatatga cacagtaagc aaacaaacaa tgttgtatcg tgaattactt
      121 tcagatgaag aatcaaaaga agatctgatt gccacaatcg ttgatatttt gggatatgca
      181 attgcaccag agtatctttt caacgtctta gcggatcaag caaaacaagc aacgttccaa
      241 ttgaacgatc taaacaaagc ctttgtgcaa ttggcatcta cctacaatca atttaatggg
      301 ttatttgatg atgtggatct ccaatcaaaa aaactaggaa cagatgagca acaacgaaat
      361 gtgacgatta ccgaagtcat caagaaattg aatgatgtag atgttttaga acacgatggg
      421 gacgtgatcg gggatgcgta tgagtttctg atcagtcaat ttgcttcaga agcagggaag
      481 aaagccggag aattttacac acctcatatg gtttctgata tgatggcgca aatcgtaacg
      541 ctcgatcaaa aggaacgacg attctttagt gtttttgatc caacgatggg ttcaggttca
      601 ttgatgttaa acgtacgtaa ctacttaaca cacccagata acgttaaata tcatgggcaa
      661 gaactaaata caacgacata caatctagca aaaatgaact tgattttaca tggtgtcgac
      721 gccgaagaaa tgaatctccg caatggggat acgttgaata aggactggcc aacagatgaa
      781 ccttacacat ttgatgcagt agtaatgaac ccaccttatt cggctaattg gtcagcagat
      841 acgactttct tagatgattc tcggtttaat cgttatggga aattagcgcc aaaatccaaa
      901 gcagattttg cgtttcttct tcatgggttc tatcatttga aggaaacagg taccatggcc
      961 atcgtcttac cacacggagt tttattccgt ggcgctgcag aaggggtcat ccgtcaaaaa
     1021 ttactagaag atggcagtat ttatgctgtg atcggtatgc ctgcgaactt gttctttggg
     1081 acatcgattc caacgacggt gattgtgttg aagaagaatc gtcaaactcg agatgtatta
     1141 ttcattgatg ctagtcgtga atttgttaaa gggaaaaatc aaaataaact atcggaagaa
     1201 aacattcaaa aaatcctcga aacctacgct gaaagaaaag atgtcgagaa atacgctcat
     1261 ttggcaacct ttgatgaaat caaagaaaat gactataact taaatatccc acgctatgta
     1321 gatactttcg aagaagaaga accaattgat atggttcatg tcggaaatga catcaagaag
     1381 atacgacaag aacagcaagt gctagaaaaa aaacttttag aagctatctc ttccctgcaa
     1441 acaacacctg aaaatgaagc gtggttacaa ggtgcgctgg aggtgttcaa acatgagcaa
     1501 tgatacacaa ccagaaatcc gtttcccagg atttacagat gattgggaac agaggaagtt
     1561 gggggacttc gttgtagatt atgttgaaaa gacaagtgta caaaatcaat ttcctatgct
     1621 aacttcatct caacaaaaag ggatagtttt gcaagaagac tattttgcaa atcggcaagt
     1681 aactacagaa aataatattg gttactttgt actacctaga ggatatttca cgttccgtag
     1741 tcgaagtgat aatgatgtgt ttgtgtttaa ccgtaatgac attattgata gagggattat
     1801 aagctatttt tatcctgtat ttactttaaa atctgctgat tccgacttct tcttaagacg
     1861 tattaacaat ggaatccaac gacaattatc tattcaagca gagggaacag gtcaacatgt
     1921 tctgtctcta aaaaagttta aaaatatagt agcaatgttt cctagtgagg aagaacaaca
     1981 aaaaatcggc accttcttca aacaacttga cgacacgatc gctcttcatc agcgtaagtt
     2041 agatttgctg aaggaaacga aaaaaggatt cttacaaaaa atgttccctg aaaacggagc
     2101 aaaagtgcca gaaatccggt ttcctggttt tacggaagat tgggaagagc gtaagttggg
     2161 agaattaggt aaaactcaat cgggaattgg atttcctgat gccgagcaag gtggttctga
     2221 aggtactcca ttttttaaag tttccgacat gaataacatt ggtaatgagt atgaaatgag
     2281 aaatgcaaat cattatgtat ctaatgagca aattgagcgt aaaaaatgga agccaataaa
     2341 agatgtgcca gcgattatat ttgctaaagt tggcgcagca ataatgctta atagaaaacg
     2401 tttggtcaca agccctttct taatagataa caataccatg gcttatttat ttaataatac
     2461 ttgggatatc tattttggta aaatattgtt tgaaactata aatttgccaa gatactccca
     2521 agtaggggca ttgccttctt ataacagttc tgatattgag aatatcagtg ttaaggttcc
     2581 tgtaaaggat gaacaacaaa aaatcggtac tttcttcaaa cagctagatg atacgatcgc
     2641 tcttcatcaa cgtaagttag atttactaaa agaaacgaaa aaaagcttct tacaaaagat
     2701 gtttgtctag gagggaagaa gaatgaaaat tagtcaccgt gaggaagcag aagtcgaaga
     2761 acagttgatt cgtgtacttg gagaagggca taatcagtgg acgtatcgtc cggatctaaa
     2821 atcagaagaa gacctttggg tcaatttacg ccaaaaaatt atctcaaata atcaagcaga
     2881 actgaatgat tttcctttga ccgataaaga atttgaaacg atcaaaacgg aattactgct
     2941 gcgaacgaaa accccatttg atgctgcgaa atggttaaaa ggtgaaaatg ggatggctcg
     3001 gatcacgatt gaacgggaag attcgcaatt gggttctgtc tcacttatcc tttattccaa
     3061 tcaagacatt ggtggaggaa tctctactta tgaagtggtc catcaaattg caaaacgtgg
     3121 aagtaacata gaagcacggg atcgacgttt tgacgtcacc ttattaatca atggattacc
     3181 gattgtccaa atcgagctga aacaagtaac tgcaaaagat ggtgtttatc aggcatttaa
     3241 ccaaatcaaa aaatatgcag aagaaggtat gtttcggaac aatatctttt cgaccttaca
     3301 gttgtttgtg gtttccaatg aacaaaccac tcggtatttt gcaaatgcct taccgaaaga
     3361 cttgcaccct aagtttttat ttagttggcg aacgaaagac aatgaaaaag tagaaaactt
     3421 atatgagttt tgtaaacaag tgttaaatat cccggatgct catcgtttga ttgcggatta
     3481 tacaatcgtc agtgaagatc aagacaataa aaccctcatg gtgttacatc cttatcaagt
     3541 acatgcgatt caagccttgt ttattgccgc aaataaacat caatcagggt atgtgtggca
     3601 tgcgactggt tcaggaaaga cgttgactag ttttgtatct actaagcttt tagcacgaaa
     3661 atcggggatc gatcgaacga tcatgttagt ggaccgcaaa gatttagata atcaaaccac
     3721 cacagagttt acaaaatttg catcggaatt taatactggg atttcttccg ggaacgccaa
     3781 agcgaatagc ttgatcgttg gaacgggaag tgccaaagaa ttaagtgaaa ccctgttggc
     3841 agatgccaat gcaaatgttg taatcattac aacccgtcaa aaactagatg cggcgttgaa
     3901 atatgcaaag aaacaagaag agaaaaaagg aaccaatcgc ttccaaaaat taatgggcca
     3961 acatatcgtg tttgtagtag atgagtgtca tcgagcgtta agtgctgaaa atatggaaga
     4021 aatcaaaaaa atgtttccga aatccacttg gtttggcttt acaggtacac cgatttttcc
     4081 tgagaatcgg aaacaagcca aagggcaatt ggcacgtaca acccatgatc agtatggaga
     4141 ggtcttgcat acctatacca ttaagaatgc gctagaagat ggctctgtct taggatttca
     4201 agtagagcat gaaaacaccg tggagccgac ttcactagag aataaaatct atcggaaatt
     4261 aaaagaagta gaaacatatg cagagtattc atcagaacag atcaatcgta tgattgatca
     4321 aatggaacct gtcaaaaaag aaagttattt ggacccagct gtttttgaag cagatgaaca
     4381 tatccaaaaa gtcattcata aaatgttccg tccagataat gcctatacaa aatttgattt
     4441 tcgaaatggc aggccaacga aatctgcaat cttaaccacg agttccattg acatggccaa
     4501 aaagtattat cgggccatca aagagatgac gaaagagcct gattggttaa caaaagagtt
     4561 tcccaatcag ctcattcgtg aaggacgaac gatggaggat cctgattttc cacgaatcgc
     4621 tattacctat tcattagaag aaaatagccg agattcaagt gcccaacaag aagaaatgca
     4681 aaaaattatt gaggaatata atcagtatta tgatacttcc tggtcattag cagacattga
     4741 gcgttataac ggagacatca ataatcgttt ggcacgaaaa agagcggaat tcaaacaatt
     4801 tgggaaacaa atcgatttag cgattgtagt ggatcgtcta ttaacaggtt ttgacgcccc
     4861 tacgattcaa accctatttg tggatcgtaa tctagagtat gcaggattga tccaagcttt
     4921 ttcacgtacg aatcgtacgt atcctgataa gacaaaagga ttgattgtga cgtttcgtaa
     4981 gccagcgaca atggagaaga atgtcgagga tgcgacaaaa ctgtattcaa aagcaaaaga
     5041 agaatcaggt ttgatttatc caagttatca ggaatccaaa aaaagattca aacaagcata
     5101 tggtaagtta aaagaggttt catccatgga atccattgat gaacatacgc cacttgaaat
     5161 ccgtatcgag tacgtcaaag catttcaaga attgaatcgt gcttatgaat cgttagtgac
     5221 ttatgacgat tacaatgatg acatggtgaa atctccaact ttgaacaatc aaattcaact
     5281 tttggaagaa caaattggcg tgtacgagac cgtgaagggt tcattaatag aggaagaacc
     5341 agaaaaagaa ggagaagatt tttcaacgat cgaattttat agtgaaaata ccactaagtt
     5401 gtatgatatt gattcaactt atattgatca attactagga acttatgctg cgaatagttc
     5461 agatgcacga gaagaaattg aaaaagcttt ggctaaattg aataaaacag aaggagtcaa
     5521 agaagtttac cgacaaattt taaatgcatt tgatgtgggg actttagata gtaatgaaga
     5581 tatctttgtc attaaacgtc gctattttac acaagcgagt gatgatttga ttcatgtctt
     5641 ttccaatgag tggtttgttt ctgagaaaga attacatgct tctaatctgc aatatctacc
     5701 aggtgaagat ccaattccta atatgaaagc aattatcaac agtaaggatt acgaaggata
     5761 taaagccaaa catccagaag cgaagccatt taaataccca caagaaatga agcgagcatg
     5821 gcgtaaaatg ttagatgatg agttgattcc acttgagaat gaattgaggt aaacgagaag
     5881 agttaagaaa attgtacgat tcaaagatgg tagtcaagtg gaagtgtaga aaatagggat
     5941 agatgtcttt tatttgttca cattttataa cgaagtattt aagacagcta ttgaataatg
     6001 tttcctccac ctttttcatt catttacccc accccgttgt tatatgaccg tgttttgaga
     6061 ggaaccaata aaaagcatag gctaactagg ggttcctttt tcttttgttt ttttggaatc
     6121 cgcttaagtg aaatgtatct gaaatgaaac ggatataaag tgattttttg ctataatgac
     6181 aaggttttag ctgtttcaga taccatgtaa cccaaaatag tttattgaga aaataggaga
     6241 gtatttgata ctaactagta ggaaaaaatg agataaaaat taaatggaat catttcaggt
     6301 aatctcaaat aaaaaaatat acagtcctgt ggcactttac ctatgggact gtatattttt
     6361 tactctcagc gggcttgttt tgactccgtg tctgactccg tcgtattgat tttaaatgat
     6421 attcgatgac catttaacag gtcatttttg aaatttttgt ctctatatga ctcagtttga
     6481 taattattga tagatcaatt tgggtctata tcgaacaagg gaataatcca ataaaaaatg
     6541 atttagaata tcaaaataac aaaaaagagg actacctgta ttcgtcctct tttttgacta
     6601 gattttgacc agtctaattt acattctaat atattcatat gagctaattt tttgattaaa
     6661 tggtaagttt ttccttgttt gttttagttt aaactagtgt gtaatttaaa aatctatatg
     6721 gaagggtctg tggggtaata cttaaataaa ggctaggaac ctgataacat
//
LOCUS       DACDIE010000101         6424 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.104, whole genome shotgun sequence.
ACCESSION   DACDIE010000101 DACDIE010000000
VERSION     DACDIE010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.104"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(1041..1706)
                     /locus_tag="IV250_002600"
     CDS             complement(1041..1706)
                     /locus_tag="IV250_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8769835.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDF
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            1832..3868
                     /locus_tag="IV250_002601"
     CDS             1832..3868
                     /locus_tag="IV250_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAP8769836.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERVISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            3872..4510
                     /locus_tag="IV250_002602"
     CDS             3872..4510
                     /locus_tag="IV250_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8769837.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            4538..4966
                     /locus_tag="IV250_002603"
     CDS             4538..4966
                     /locus_tag="IV250_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769838.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            5423..6334
                     /locus_tag="IV250_002604"
     CDS             5423..6334
                     /locus_tag="IV250_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP8769839.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
BASE COUNT         2224 a          976 c         1182 g         2042 t
ORIGIN      
        1 acatcaaggt tcctagcctt tttataataa aatgaagcga aaataatttc ttttaaagag
       61 ccgagattat cagctatata agagtgaatt tgatgcaaaa aacagagccg aaacagtctt
      121 ggtgttcgta gctctgctta tatgtttcga atttaagaca aagagttggc aaaagatgtt
      181 gataaggaag ataagataac tgtgttatat aaactaggta actaactgta acaagctaat
      241 tttaagagat tcttattaga agacgtttag gaatataact aagaaaatga ttatacaggt
      301 aattgtcatt tcaataatgt tttctaattt tcgcgtcttt gttttaagaa aaaggccaga
      361 atcagttgga aaaaagttcg tacgatagtg aagaggggca aaatttccta ctaatattcc
      421 cactaacaag agataaaacg gccaggttgg tgtgattcct ctataaaaaa atgcccatgc
      481 taccgaaatc ccatagatta acaactgaac aatttttaca atgaaaaaat tgttctgcca
      541 ttactcaaag tctcttcctc cttgtatcgt ttatttgttt cattttatca ttatgatagg
      601 cgcttgaaca atccattttg tattttattg agtactcttg gataaactac cagcgattat
      661 catatttatt tatgttactg taagccatca tataaaagta aatgagcaga tcatagaaat
      721 ataaaaaaga actaaaattg atctaaatta tgtgcagctt gactacgtac caatctatat
      781 tacttactag attggtcgtt aatctattcg gatcgaatga gcaaaatatt tttgtgaatg
      841 tccatccttg attatgattc caaaaagaat cattgggagg ctgtggcgta aatattatac
      901 tataagccag aaatacctat cattctgaac ttttttattt aggaactttc atctcaaggc
      961 tttttcaatt aatttgtacc cctgtttttt ggataagatc atgtgcattg attttttcta
     1021 tctctacgtt catctcctac ttaatgccta tctggctcct gagggcttct ttttaaggcg
     1081 tcgtcatagt cttggctgag gatttcttgg taatctgagg gaatggcttc ccaagtgagc
     1141 aggcgaacgt tcatgacgtg ttttcctttg tgtttgatta cagcatggtt tttctctttt
     1201 tctacctctc cagcgtgaaa agacagccat gtaccatggc caaagatatc attacatacc
     1261 caacgttcga tggaattgtc cacgtatcct acagggagtg tggtatagcc gatttgtggt
     1321 ttcaaaaaag gattgaatcg atcgatcgtt tcatttttgt attgataatt gataagaaaa
     1381 agatagacca aacaaaagac gagcagaaag aaagatagtg tctttttgag tgagaagggt
     1441 tgttttgatg aataagattt cttgttaaca agaggttcat ctggcaatgt tggttctatt
     1501 gattcggtag ctgattcaat agaaggaaaa tcaatcggca gacctaaagt atgatgtaaa
     1561 agtgacagca tttcaaagtc tggcattgta cgagaatttt cccaactaga aatcgtttta
     1621 tcggaaacac caattttttc gcctaattct ttttgcgtaa gcttacaatt tctgcggtaa
     1681 gttcgtattt tttctccggt gttcatcttc ttctcctttc gattctacag tatatagatt
     1741 tattctacat gacctataca cttgatagat acgttttttt gatgaaaaac ggtatgattt
     1801 tatcaaagaa tcatttaagt aagggagagt ggtgataatc aaaaaaaagt tcacagtttt
     1861 actcacaatc atagtgaccg gtatcatgag tggttttgcc ttggttagta taaataaaca
     1921 ggtactctat cggacaaatg aatttttcca tcaaacagat acttatactc cgattcgatt
     1981 gccacaacat tttattgggc aagaagaata tgagcagcta atcatggtat ttgaaaatgt
     2041 ctcatcagaa actgagcttt cttatatgaa aaaaacagta aatgaatcca atgaagtaga
     2101 tacatcttgg aacttcaaga tacagccaaa gcttgaaatg acattgtatt atcaagggga
     2161 tgtcaaatct tctcacgaga aggtacccta ttctaatacc tcctttcgcg taacgtccat
     2221 tgaagaatcg atcaaaataa aagattttga aggtgaattt ttcttgatga cgagagaaaa
     2281 agaagcctat gaacgggtca tttcactatt cactgatcga ttcaatcaag catttcacac
     2341 tgattatgcc actaatattt ttgaagactt tgaaaatagc tattcgtatt cggttatttc
     2401 aaatacagac gtatataatt atcgagtgta tttaactatt ggattgattc ttttattcct
     2461 tttgttatca tcctttttct tattgatgaa taaagaaatc agtttattgt tgaataatga
     2521 cttttcatta catcaagtat tgtggtgttt attggggaaa aagaaaatca ttattacatt
     2581 cataattgca ggaatcattg gtggttttat ttttatgtat tataaggagc aatttcttta
     2641 tttgttccag atgtacgttt tcttgttcag cggaatatat ggtatctctt tgatttctct
     2701 tttattagtt gaaaaaatcg cttcgttaag agaagcgttc aactatatcc aatacattct
     2761 ttttacattt ggattgttgt tttctcttct tttgaccgtt gccaatgtcg aattagcaac
     2821 tatcgttttt tcttcttcac agttagctgt ttcccatgaa ccaccaaaag aaatcgctgg
     2881 tgaggactat catgtatttt atccagtaac catcgggaaa aaccacgtgg attttattta
     2941 ttccaatcgt tttgcatcag cagcagatca ggaattatat gaaactttga ataaaaacgg
     3001 aagtatatta gtcaatgtat cagattactt gaatgaagaa aatgaacagt ttggcagagg
     3061 gataaaaata aatctgaatt atcttaagaa atttcctttg attgatgtct ctggaaggtt
     3121 gatccaaata accgaaaaag aaacgcaccc tgttatattg atccctgaac gatatagaca
     3181 tacagattta aaaaatgacc tggcgcaaat aacagaatat tatcaagaaa tcagtgaaaa
     3241 agaaccgaaa tttctattta taaaaaacaa tcaacccatt tatacattta ttccttctat
     3301 cccctggatc gaacattatc cggttgttga aatactgaca ttgaaaaaca gtatgtattt
     3361 ggaaagaaat attctcagcg gagaaatcta tccaccgtta aagatcaaga taggctcttt
     3421 gtcgaaagaa aggttatttg agctgattga agaaagtcag ttacgtgata acttgcagtt
     3481 atcatttccg tatactcaga ctgaagagat agctgtcaaa gtgttaagca gatcatttca
     3541 ttacttgata cagatatttt tactcacttt cttttctttc ttcttgttga gctatgtcat
     3601 gttatgcata tattttgtct acaattgtcg gaaaatcgct atctttcgtt caagtggtta
     3661 ttcgctcttt gaaacgtata aagatttctt catgatgaac ctcatcaaat ggggaacgac
     3721 aagtgtaatc ttcctttttc ttatagaaag agagcctaaa tatcttttta atattttttt
     3781 ctttagcttc attgatttta ttctttcagt tgtttttatt ttgtctacag aaaagaaaag
     3841 tcagttattg ctgatgaatg gaggttaaca attggagccg ttgatagtga taaaaggtgc
     3901 gaaaaaaatc attcgaaaca aaacgattct tcatttggat cgtttcactg cggatgctgg
     3961 ggatcttgtg actgttgttg gagaaagtgg agcaggtaaa agtacgttat tgaatatatt
     4021 aggattgaat gatcagttca gttcaggaca attctatttg ttcgatcaag agacgaaaca
     4081 attaactggg agagagaagc gcttgataaa aagaaatacc atatcttttc tttttcaaga
     4141 ttttgggttg attgaatatg aaacaatcga ttttaattta gatattggat tgaagttttc
     4201 gaatttaaat gtttcagata agcgaacagc aaagtatgag gcattgaaaa aagtaaatat
     4261 aaacaagaag ctaagtacgc ctatctacac attatctggt ggtgagaagc aaagaatcgc
     4321 ctgtgcgaga gtcttattga agaatagcaa aatcatttta gccgatgaac cgactgggtc
     4381 attggataaa agaaataagg ataaagtagt tgaaatctta tcgcaacaag cttcccaagg
     4441 tacgttagtt atcgtagtca cgcatgatcc atatctgacc aaaataggaa acaagaagat
     4501 catgctttaa aaatcaaaaa ggaaaggatg tcgttttatg aaaaaagcag ttattatttg
     4561 tgcactaggg ataggttttg ttggaggaaa cgctatgtca tctgaagtgg aagcaaattc
     4621 agttcacggt gaaatctatt acaaatcttt aaagccagga gagattcatt ttgatgctaa
     4681 aggtaatatt attaagtcaa atttaccagt agaccgggaa atttctagaa cagcaattaa
     4741 tcatagctct ggaagatggg tttattatag tgatacaact tgtacgagaa aaacaggtca
     4801 ttctaaccac tatagtcgtg tctataatcg tcacggtgca aaagcgaaag ttggaaactc
     4861 tattcgatca gcaaatgcaa caaaaggagt atgggcctat gcaagtgcga caggaaatag
     4921 aactgacaca ttctcatgtt ggtacaatcc aaccggttgg tattaagtat ccaaaagttt
     4981 atataaaata gaaaaaacat ataatgaaac agcgttaaaa gttatttttg gtagttttaa
     5041 tatattcatt tttagttgta taaaaatata tcataataaa aacaacaaaa aaatgtctgg
     5101 aaaatcgaac ataaaagtgt ctgatttgaa tatcccagca gcggaagaag aaaaacaaaa
     5161 tcgataacat ttgttctaag aattagttac gttaaatacg gaacgcaaga tagacttgaa
     5221 aaacggtaac aaaagaagta gtatattaag gtgtgatgtg ctacttgaca tttattctgc
     5281 tgagcgaact ttctggattg tgtagcctta tatctattca ggacgtatcg agcagattag
     5341 taaaggtgtt cttgatgatt ttcatcttga acacctttgt ttttagcaat tcgcaaaaaa
     5401 cgaaaagtag gagaattgat aaatgaaaaa aagattttta gcacttgcca tcgtactagg
     5461 cactggctta ttgtcaggct gtacgaatgc cggtgaaaaa acagccgtta gttataaagg
     5521 cggtacaatc agcgaacaag aagtaatgga tagcttgaaa aaaatgcaag gagcagattc
     5581 cgctgtgcaa cagttgatcg tttatcaagt atttgaagat aaatatggag atgacgtatc
     5641 aaccaaagaa atcgattctc aatatgatca aacaaagaaa caattgggtg attcatttga
     5701 ttcacaattg aaatctgcag gttacacaga acaaacattc aaagacagta tcaaacaaag
     5761 tctagccttt caagaaggtc taaagaaaca tatcaaattg accgatgaag atttaaaaac
     5821 ggcttgggaa tctttccatc cagaagtgga agctcaaatt atccaagtag caagtgaaga
     5881 tgatgcaaaa gatgtgaaga aagctgctga taagggagac gatttctcta aattagccaa
     5941 agacaagtca actgatacta cgacaaaaga agatggcgga aaagtgaaat ttgattccac
     6001 tactacaacg gttccggcag aagtcaaaga agcagccttt aagttgaaag atggtcaagt
     6061 ttctgatgtg atcacttcta ccaatgcttc aacttatact acggaatatt atgtggttaa
     6121 aatggtgaaa aaccaaaaca aaggcaacga catggataaa tacaaaaaag aattgaaaga
     6181 aattgcaaca gacacaaaat taagtgacag taccttccag aataaagtaa ttggcgaagt
     6241 attgaaagac gctaatgtaa aaatcaaaga caaagacttt gaaaatgttt tgagtacatt
     6301 cacttcagat tctagtacag caagtagtaa ataagcggta taaataaaaa atatgaacta
     6361 tttaaatcaa taaaactgtt catctaaatc tttttattag aactaaatca acagtagttt
     6421 aatt
//
LOCUS       DACDIE010000102         6408 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.105, whole genome shotgun sequence.
ACCESSION   DACDIE010000102 DACDIE010000000
VERSION     DACDIE010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.105"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            361..642
                     /locus_tag="IV250_002605"
     CDS             361..642
                     /locus_tag="IV250_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769840.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESD
                     FDRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
     gene            644..1060
                     /locus_tag="IV250_002606"
     CDS             644..1060
                     /locus_tag="IV250_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769841.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
     gene            1682..2836
                     /locus_tag="IV250_002607"
     CDS             1682..2836
                     /locus_tag="IV250_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HAP8769842.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYIYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            2830..3252
                     /locus_tag="IV250_002608"
     CDS             2830..3252
                     /locus_tag="IV250_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769843.1"
                     /translation="MVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            4422..5390
                     /locus_tag="IV250_002609"
     CDS             4422..5390
                     /locus_tag="IV250_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP8769844.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKSDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELL
                     QQLEKLEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
     gene            5407..5931
                     /locus_tag="IV250_002610"
     CDS             5407..5931
                     /locus_tag="IV250_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP8769845.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINE
                     TGQKAIKSMFFNENENSNQQLVGEKTKTENHKNANQVPELEVLSLLEQQLNQKDMQLQ
                     EKDEQIKQMQKLLDHQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIE
                     KQSKAEKKWWQFWQ"
     gene            6107..>6408
                     /locus_tag="IV250_002611"
     CDS             6107..>6408
                     /locus_tag="IV250_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HAP8769846.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT         2414 a         1095 c         1046 g         1853 t
ORIGIN      
        1 ttaataaaaa ttgctggttt tattcttctt tttgcttttg cgagcagagc aacatggacc
       61 catgttgcga tattttggtg gcgccccaat ccccgttttt tcgttgaatc ttactcggac
      121 tgcactcgtt ttacagctga tttttctgaa ttttgaaaga gcataaggaa acgtggtatc
      181 actttcttac gaaaggtgct agataggctg tgcctatcac gtttctcaga aaaaaatttg
      241 ctatcacttt tttgccattg tggctaggct atgtatagtg ttttggcgaa aaatagctat
      301 acaaatacta tatttgtatg gtatacataa attatacgct gatacgaaag gaaaatttcc
      361 atggacattt tagttcgaaa tatcaatccc aaaattattg caagaataga taaacgttgt
      421 caggaactca caaccaaaac gggcaaaaaa tggagtcgaa ataaatatct aaaagttcta
      481 atcgaaagtg attttgaccg tcaacttatg aattacaaaa aagatcaatt cgatttatta
      541 ctcgaaaaat ttattgccat tcaaacagaa aatacgaaca tactgaatga ctatgttcac
      601 acaaacaacc aactcattga aacacttatc ggaggaatat aacatgagaa aaacttctgt
      661 tcaaaaacac gtaacccttt atcccagtca cgttgacgtt ttagattcca tcatcgaaaa
      721 aaatccagac gtcaacaatt attcagaagc cattcgtttt ttatgtatga gttatgaacc
      781 tgataccaat acaaccacaa ccagtaacca agcaaaactc aatgccatgg gaaaagaaat
      841 ttctatcatg acagaaatca tggcagaatt tgctgattta tatctaaaag atactggtgt
      901 tcttatcggt acaaattcta aagtgtatca agaagtgaaa gaaaaaattg aaaatcaact
      961 caatccttcc cgacgaaatc aacccaaaaa tcgtcaaaaa gaaaccaatc aaacctttac
     1021 ttctggaaat tttgacccaa caaactttct aaaaagataa aaaaagaaac cgatccctaa
     1081 aaaaattcct cgatttcttt ttagggatca gtcttccagc aagatttgca agttggtacc
     1141 aacttgcgaa aaaaaagaaa aatccgctta tgcaacttct tctttttgta ttttgaggcg
     1201 ctaccccaat ccccaagtat cggttattta atagcgcgga aatccaaaga ataggacgta
     1261 ccctgaaaca aaaaaagact gctctcacga gcagcccaaa atgagattat cctattcatc
     1321 aacataaaaa tattacaaag tgaagaacta gagaaagaca aaaaaagcat atcaaatcct
     1381 ctcaaaaata acaagtcttt aaaaaatgac agataaaaat gcacaaagaa ctaaaaaaga
     1441 gaaatcgcgt aaaatcaagc attagaagct agtaactagc tagaccaatt aaaaatcaca
     1501 aagtgagcgt ctctatccat tggtagaggg gctacttggc atagtgtaca ttatgttaac
     1561 taacgctata aataacgatt atgttcccaa aacaatattt tatgaactat aacaaaaatt
     1621 tgttatcgca tattgtatat tttaattata ggtgtatacc acttaaattt aacacaaagg
     1681 cgtgattatt ttgaaaccca aaaagaaaat ttatctaggt ctcctctttt ccttgatgat
     1741 tggaattttt agtataggaa ttacaaatac ctatgcttca gaaaattcta atttcaatat
     1801 caactcccaa ccaattgaag aacaagcaat taattttgcg aaaactgatt ttgaacactc
     1861 cataacttca attcttttaa tggaaaataa caccgaaact gatattaact atactcttgg
     1921 ttcgccattt aaaatgctcg atggagaaaa tacggcaaat agttctattt tctttccagt
     1981 attttcagac gctgataata cgattaaata tatttatacc gtcaaaaaaa atacaaatgg
     2041 agaatattca ggaagtattt cacaattttt agcaaaagaa cttcaaaatc tagtagatag
     2101 taatataaca gatacaacct tattttttca agataatagc atttattatt ccgataatgg
     2161 acaaattaaa ctactttgga aatcaccttt aagtacagaa aaaaatggtg tttcttcctt
     2221 tacagaaaaa aatgatctta tatccgtaaa tataaaaaat aaaattaaaa atttctctaa
     2281 aaaacaaaat tttcctgcgg ttaaacgtgt aaaaagtgtc tcctccggag ataactttat
     2341 tatgattaat tggcgtatag cagaaaccca aacaagtttt ccgtggtgtg ctgcttacgt
     2401 agaagctgct atcttatgta acaaaacaga tgtacgtgct acttcagcta gagccattat
     2461 gcattatacc tatcctaatt taaacaacag tcaaattgaa tcaaaaagtc ttaatcaaga
     2521 tcaaataata aaatatgcaa attactgtgg tgtttatcct aaaaaagtag cacgaatact
     2581 aaacctttca gaggtccaaa cacaactaag aaaaggaaat gccatttata tgggaactgc
     2641 tggttatgga aattatagta acagccgtca tgcacttgca ttatttgggt gggtcaatca
     2701 aggtggttat caaacttact atgtgtggaa tccttggtat aactacccaa ctgtaataag
     2761 tgcaaatgca ggaccagtta caattcctgt acctggaggt ggttttactt gggacaatac
     2821 cattacaaat tggtaaataa acccattgct ttagttatgg gtctagtgtt agttcttttc
     2881 ttaactagtt gttcaaaaaa acaagaaata caaaagactg atctgattcc ttttctagga
     2941 acttggcaaa ctaaagatca ttcagaacgt ctagtaatca ctaatttaaa tacttcaaaa
     3001 aatatagaac aatataccat cttagtaaaa gagaacaatc ataaaattca aatcattgat
     3061 aaaaatttaa atataaatga acaagttttc atttacaaag caacaaataa tttactaaca
     3121 ctgcgctcta ataaaaaaca attagattac acttttagtt tggattctcc aaaaaagctc
     3181 gtatttattt tgacaacgaa tgatgggaca gaagggacag ctaaaccaat tgaatttttt
     3241 aaagctgatt gatgaatagt tgtttgatat tacaaaacaa taatttcacc gaaggtgaaa
     3301 caagcgaaac tttacaaaaa gtggctagca tttgctagcc ttttttgtat tgtggagcgt
     3361 gggttttgag ggggattcaa gggggagctt gcgccccttt gaaaagcggt tatttgcacc
     3421 aaatacgtag gtatttggct agcaaatata gagcttgcca aacactttag tgagttttgt
     3481 agaaagtacc tttaaaggta ctttcacatt acgaacgata cgtccgtagg acgtactgaa
     3541 agggtgggat ttatgagcga actaatgcaa aatttggcta gcaaaaaaag ttaaaaaata
     3601 tatgctaata atcgagcgaa atgaacactt tttcaagtga gcgaatccgc catgacaaag
     3661 ctgaagcagt ttatttcagc tttgtcatgg cgtgtgccta gacttttaaa aaagtcggca
     3721 cacgagcaaa tatctttatc aagctctaat ttgcgtttta agacgtttga aacgtctaag
     3781 tatataaata tataagagta ggtttaagag tgcttaaatc gaattttaga gggcattcag
     3841 acaactttag ggttgttagg gcttgccctg accgcctgta aggcgcttta agaacaagat
     3901 aagggattta tactaagact gtcttaaaac gtctcaaacg aaggctcata gcttctcaaa
     3961 gagttcctta tgaacatttt tagcaaagga gccggaagcg actgcgcagt ataatgtgag
     4021 agcagagcga tcacaccttt ttaagcgact gtagggagta agattcccag ttgagaaaaa
     4081 tgcgcgaatc tgtttggaga aaaaagaagt ggttttttag gcgggaaatt ttgtccccct
     4141 cttttataat ctctttttaa acctcttttt aaacctcttt accgtcggct atatccttac
     4201 tctcccaagg gaatgacgct tttatctaca tcgttttgtc tgtttatcta catcgttttg
     4261 tctgtttatc tacatcgttt tgtctgttta tctacatcgt tttgtctgtt tgtacattga
     4321 attagtacat cgacgatgtt aatataagat gtagataaac aggcattttg tatttcataa
     4381 cgtgtatctc ttgtgcgagt aagaaaggag ctattttaaa tatgtctaat gaaatcgtaa
     4441 agtatcatca tgaattaaat acaattccgt tgcgaaaatt tacaccagtc gaaatgaatt
     4501 tgttcttttc tatcgtatca agaatgcgtg atgtaggtga taaaaaagta caatttacat
     4561 tcgagcaatt aaaagattta agcaattata aagcaactgc aaatgttcga ttcatagatg
     4621 atttagaaac aacgtatgac aaattaatgg atttacgttt tggtagaaga agtgctgacg
     4681 gtttgcaacg tgaacgtttt gttttattta atcaatttaa aatcgacgga aaatctgata
     4741 ttccttttgc tgaaatacaa gttcatgaaa aagccttacc cttacttaat aatcttgaag
     4801 aatgggtgcg ctattctctc caacaattca atgaattaga aagcagttat tcaaaaacaa
     4861 tgtttcgatt actaaaacaa tacagaacca aaggtttcgc ttatttttca aaagaagact
     4921 ttcatgaatt acttgatatt cctaaaagtt acaaccaagg tacgattgat caacgtgtgt
     4981 tcaatccaat acgacaagaa ctaaccgcaa tctttaaagg tttaacaatc aaaaagaaat
     5041 acggaaaagg acggggaaag cctgtcattg gctatcaatt tacatttaaa cctgagataa
     5101 aaaaatcaga cgactttaac aaacatgccc caacaaaaga agaacaaaac aaagcattag
     5161 aactagctaa ccaacaagtt caatctgaaa aagcacctac ttcattacaa gaaaaaatca
     5221 aagaacaaaa gcgaaaattg gaacttttac aacagttaga aaagttagaa agagaacaaa
     5281 atacccaaaa taaagaaaat aatgcacaaa aatcagagat aaattccgat attccttcag
     5341 aactaaaaaa agacttatta gacaatcttc aaaacttatt caaagattga ggggttttta
     5401 gattttatga cagaaaaaac aatcaaagaa cttgctgaag aattaggagt tagtaaaaca
     5461 gcaattaaca aaaaagtttc tgacgaaaat cgaaaacttt ggttttcgaa aatcggaaac
     5521 aagtttgtga ttaatgaaac cggacaaaag gctataaaat caatgttttt taacgaaaac
     5581 gaaaactcaa atcaacaact agttggcgaa aaaacgaaaa ccgaaaacca caaaaacgcc
     5641 aaccaagttc ccgaattaga agtcctttct ctcctagaac aacagttaaa tcaaaaagat
     5701 atgcaactac aagaaaaaga cgagcaaata aaacaaatgc aaaaattact cgaccaccaa
     5761 caacaactca ccctgcaagc caaccaacaa atcgagaaac tgcaggaaca actacagttg
     5821 acttatacgg aagaatcttc ggaaaataaa tcaacgactc tttctgaaga aacagaatcg
     5881 atagaaaaac aatcaaaggc ggagaaaaaa tggtggcaat tctggcaata aaaccagcaa
     5941 tttaacagaa ttaggcagaa gtcgcccatc ctctataggt gggtgatgaa tgccgttcgg
     6001 tatgagggtt accgttggta actcgaagac cgacaactaa ggcgtattgc ctttttattt
     6061 tgtcccatgt tacaatcagt ctatacaaga aatgagttga tataacatgg aattagatac
     6121 aaataatcat tcagtatttc ttctttatta ccaccttgtt ttagtaacga aatatcgtag
     6181 acaagtgatt gatgatgaaa tatctgatta tgccaaaact acttttgaaa gaatttcaga
     6241 atcgtatcac attactttag tggagtggaa tcatgataaa gaccacgttc atattatgtt
     6301 caaagctcaa cctaaaacag aattgacaaa attcatcaat gcctataaaa gtgccagctc
     6361 aagattgata aaacgtgact ttccaagagt caaacaattt ctttggaa
//
LOCUS       DACDIE010000103         6321 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.106, whole genome shotgun sequence.
ACCESSION   DACDIE010000103 DACDIE010000000
VERSION     DACDIE010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.106"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(259..822)
                     /locus_tag="IV250_002612"
     CDS             complement(259..822)
                     /locus_tag="IV250_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769847.1"
                     /translation="MQNHEITTAKKAIGILSIIVILCCVVLFQKKNAYDNNVKELEDL
                     KSKQIEVVKNNQKVKAMQEAQYKELGNAEIKEQSRKFFNLFYNWNSWEKYTKNMKEIQ
                     KEFPQIVKSSPVDISGTMVGTGNSPISSYTADYYTTNQKGKMVELITQTRNTPTTATT
                     TIWRGVTSIGENQLFYLEDLDSYVRAE"
     gene            complement(809..1336)
                     /locus_tag="IV250_002613"
     CDS             complement(809..1336)
                     /locus_tag="IV250_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769848.1"
                     /translation="MNSKVKDTIIISLLSGMLLALLVGILFFNKQNQDIEAEKHSVHI
                     EDKLTFFKEVKPNEVDGLRKDLENDIQEMLNGSVQNKFADQSGEEFLRKLFIVTGKPP
                     ITDKSSEKEKIEYYKPFTFKLTNFGIKTSSSGLYEALTDFEVQYNGKKVYDTTLTITL
                     NNDKKVTGGAFYAKS"
     gene            complement(1359..2696)
                     /locus_tag="IV250_002614"
     CDS             complement(1359..2696)
                     /locus_tag="IV250_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAP8769849.1"
                     /translation="MLMVQFTGIAMSVITINPQVIHADGIATYDAQFNCTTEGSGSGT
                     DSDGNDTGSGAVTGDVAKNIEDIYKYMNGEYGLSGASIAGILGNWKQESGIDPRAVEG
                     YYGDGQREVDFIKQFTDGLMGIGYGQWTAGRHTALVDWSKKNNGGSWWTTEAQLDFMF
                     KGDGGFVGILKDYALNASDDVVENAVTFHKNWEISADSESAVIAVRGENAKEIWKYMK
                     SKGMDGAKDESKINKISGGSSTGDGSNNSSSSASGTVEDACATTDDANSGSGSIGKEG
                     KINGKKGQVIANMTYEEALKKYGKAITLPKFKEPDWSTSPFNGIYQGQCTELTWAYMS
                     QLYDGNQPTTGNGGYVWESYKSAGAKITNKPIVGYGFSASDGWLWAVGDAGHTGVVIA
                     VFEDGSFLCANFNVPPTQAPTRGVSVTLIDGVDGSDNVHFFSGVGKAKFEIEK"
     gene            2816..3295
                     /locus_tag="IV250_002615"
     CDS             2816..3295
                     /locus_tag="IV250_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769850.1"
                     /translation="MTDKTSDLPKLAPKTSRVKSKKDGKTKNAVKKKSVKETSSLLLD
                     IPMSSFDDNTDETSVKIATHVGMNNQNDSFYNLLNNLRELEDKKNQVIASKAPDLTTD
                     LDAEIIQIKQDERSVKSLLKTYFDGFDKELAPYSQAILTKPIQAPHISISLQKKSDD"
     gene            complement(3891..4421)
                     /locus_tag="IV250_002616"
     CDS             complement(3891..4421)
                     /locus_tag="IV250_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769851.1"
                     /translation="MSQNISAVKQLIEEDLETTVQRLAERIHFANYQTISNWSSRNRN
                     PEEIPMKFFYRIGKEYGVDPIKIFEKTMIYYYHGTPNEVLEHLLEEAKEKSSILELSQ
                     NDKVIQVKCVDPTGKIIVGFDIERKSKIDFITQLKKINNLDILTLRPDHEQLFMSFKK
                     QLKEIQKRYNKVFPNA"
     gene            complement(4437..4802)
                     /locus_tag="IV250_002617"
     CDS             complement(4437..4802)
                     /locus_tag="IV250_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769852.1"
                     /translation="MSSLKDDYIQAKQEYEKIQMLKSEKVELFLTQKLAEDHIANLDA
                     KTLEEYDRQFNQIHHNIILLVEQAKRRFEKAEYDLLDQGIVCLDKGLRRYYGAELKKQ
                     NVDRNALKHEILELIMQRI"
     gene            complement(4808..5551)
                     /locus_tag="IV250_002618"
     CDS             complement(4808..5551)
                     /locus_tag="IV250_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769853.1"
                     /translation="MKEISANEAHIPQKNQVGIYKVLHGEGIYKIQLLTGYNNGANGK
                     WLKATSIRELRSEVEKIRLGLTSEEIAKLKGNNSFVIEKEKEKHLLYRELKIEEYYEI
                     DLPEKLRIIDQRSEEKWKTEIYPSLNDYKTSGKPMYNYTGFLTYTQYKYAFRKLKGIK
                     QAEIALYEMKKVDRLKLIQDLVQNTWNQAIVELEKENGKILTNKYLIVNEIEELIGLF
                     SCEKGEVFLKSSPTYSILDSPYQFIQARR"
     gene            complement(5682..>6321)
                     /locus_tag="IV250_002619"
     CDS             complement(5682..>6321)
                     /locus_tag="IV250_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769854.1"
                     /translation="NNNKDTKSSSTMVTKNSTTSASTTMSSMTENNAEKYQKYEDINK
                     GQKPDEVYNNFINTVKNDENYGNDPLQNKNLTIVLYKPDCPDCQKAESKIMQEIKEGF
                     SYAPTLMHDITINKPAYEYYAVAVDITDGVPEWVQNIPGLADENNQYHTPTVAVVTPK
                     KVNGTLYWDVMGMTTNTTDEMIESTTKYYMDLGAYKLYQQSEPVMDDQSKVK"
BASE COUNT         1821 a         1267 c          843 g         2390 t
ORIGIN      
        1 gtctttaata gttttatttg actttttaag aaagtattta cttttgactt tttttacgtt
       61 ttacaacata tacgtattgt aatgttcagc tcaatttatt ataatctaaa tgaattgatt
      121 aataaaaatt ctgaggttcg ggttttttat caattctttt ctgtttgtgt ttgtgatgaa
      181 acaatccttt ttcggtaggt tttgttatca tcgtcagcac aaagagctaa cgttggtacc
      241 gttagctctt tttttatttt actccgctct tacatagcta tctaaatcct ccaagtaaaa
      301 caattgattt tctcctatgc ttgttactcc acgccaaatt gttgttgttg ctgttgttgg
      361 agtatttctt gtctgtgtaa tcaattcaac cattttacct ttttgatttg ttgtatagta
      421 atcagcagta tagctagata ttggactatt accagtgcct accatcgtgc cagaaatatc
      481 aacaggacta gactttacta tctgaggaaa ttctttttgt atttccttca tattcttcgt
      541 atatttttcc catgaattcc agttgtaaaa taaattaaag aatttcctac tttgttcctt
      601 gatttcagca ttccccaatt ctttgtattg agcttcctgc atagctttca ctttctggtt
      661 attcttgact acttcgattt gtttactttt aaggtcttcc aattctttga cattattatc
      721 ataagcattt ttcttttgaa atagtaccac acaacaaagg atcacaatta tggaaagtat
      781 tccgatcgcc tttttagcag tagtgatttc atgattttgc ataaaaagca cccccagtca
      841 ctttcttatc attatttagt gtgatggtta atgtggtatc atacactttt tttccattgt
      901 attgaacttc aaaatcggtc aatgcttcat acaacccact actagatgtc ttaattccaa
      961 agtttgttag cttaaaagta aaaggcttat agtactcgat tttttctttc tcactactct
     1021 tatcagtaat tggtggttta cctgttacaa taaatagttt tctcaaaaat tcttcacctg
     1081 attgatctgc aaacttattt tgaactgatc cattcaacat ttcttgaata tcattttcta
     1141 aatctttccg taatccatcc acctcatttg gtttaacctc tttaaagaat gtcagcttat
     1201 cttcaatatg cacactgtgt ttttcagctt ctatgtcctg attctgctta ttgaagaata
     1261 ggattccaac aagcagtgct aataacatac cagaaagcaa agaaatgata attgtatctt
     1321 tcactttact attcatctat atcgtccttc attccaattt atttttcgat ttcaaattta
     1381 gcttttccta caccgctaaa gaaatgcaca ttatctgatc cgtccactcc atcaatcaaa
     1441 gttaccgaaa ctccccttgt tggagcttgg gttggtggaa cattaaagtt agcacataga
     1501 aatgacccat cttcaaacac cgctataact acaccagtat gccctgcatc gccaacagcc
     1561 cataaccatc catctgaagc actaaaccca taacctacga ttggtttgtt cgttattttt
     1621 gctcctgccg atttgtagct ttcccataca taaccaccat ttccagtagt cggttgattc
     1681 ccatcgtata attggctcat ataagcccac gtcaactctg tacactgacc ttgatatatt
     1741 ccgttaaaag gtgaagttga ccaatctggt tctttgaatt ttggtagcgt gatcgctttc
     1801 ccatattttt tcaaagcttc ttcataagtc atattcgcta ttacttgacc ttttttaccg
     1861 ttaatttttc cttctttccc aattgatcca gaaccagaat tagcgtcatc cgtagttgca
     1921 caggcatctt ctactgttcc tgatgctgaa cttgatgaat tattcgatcc atctccggta
     1981 cttgaacctc cggatatttt attgatctta ctttcatctt ttgcaccgtc catacccttg
     2041 cttttcatgt acttccagat ttcttttgca ttttcaccac gtacagctat tacagctgat
     2101 tcggaatcag ctgaaatttc ccagttttta tgaaaagtta ctgcattttc tacgacatca
     2161 tcagaagcat ttaaagcata atcttttaat attcctacga atcccccatc acctttgaac
     2221 ataaagtcca attgggcttc tgtagtccac caagaaccgc cattattttt cttagaccaa
     2281 tcaactaatg ctgtatgtcg cccagctgtc cattgaccat agccaatacc catcagacca
     2341 tcagtaaatt gtttgatgaa gtcaacttct ctctgtccat ctccgtaata cccttctact
     2401 gctcgaggat caatacctga ttcttgtttc cagttaccta aaatacctgc aatactagca
     2461 cctgataagc catattctcc attcatgtat ttgtaaatat cttcaatatt tttggctaca
     2521 tcacctgtta cagcgccact tccagtatcg tttccatcgc tatctgttcc tgacccacta
     2581 ccttcagtag tacagttaaa ttgtgcatca taagtagcta tcccatcagc atgtatcact
     2641 tgcggattta ttgtgattac actcattgca atccctgtaa attgcaccat taacataagc
     2701 aatgtggcaa tctttctctt agattttttc aataactacg cccccaaaaa gtgtactttt
     2761 ttatgttatt atgataatat aataacataa attaatagaa aggaaaagct aataaatgac
     2821 tgataaaaca agtgatttac ctaaactagc tccaaaaaca tctcgagtaa agagcaaaaa
     2881 agatggaaaa acaaaaaatg ctgtaaagaa aaagtcagta aaagaaacat cctcgttact
     2941 acttgatatt ccaatgagtt cttttgatga caatacggac gaaacttctg ttaaaattgc
     3001 aactcacgtt ggaatgaaca atcaaaatga ttctttttac aacttgttaa acaacctaag
     3061 agagttggaa gataaaaaaa atcaggtaat cgcatcaaaa gctcctgatc tcacaaccga
     3121 tttggatgct gaaattatcc aaatcaaaca agatgagcga tcagttaaat cgctactgaa
     3181 aacctacttt gatggttttg ataaagaact agctccttat agccaagcaa tcctaactaa
     3241 accaatacaa gctccacata tttcaatttc cctacaaaaa aagagtgatg attaaacaat
     3301 cattactctt ttttagattt ctggtaagcg ctgatcgcaa aaaccatcaa caatattgat
     3361 tcaaaaaata tgttcattcg tgtctctttt ttccattata gatttcccac tattccatta
     3421 aatcgaaagt cacaccgatt actacaaaga ttaacgtgac cacattcaaa aacattaaaa
     3481 gatatgctga taatctcatc atttttttag ttcacttact ggtaatctaa caattctttg
     3541 aacatattca cgatctatat tttctctgac ttcctcactc caataatgtt gaccgtaata
     3601 gcatttttct attacgttgt tagcagaaac ttcaaaatag gattgttccc tttgtatcct
     3661 cataccaatc atcatagcgc aggaaataac tattaataac aatcctgttt ttaatctatt
     3721 attccacatg cagtttccac atccttatct tgataaaaat cctcttgacc ctctccaatt
     3781 actgttttaa atataaccac caaaacaaga tcatgctaag aaatccaaga gtatataaga
     3841 tgatacattc tttgttaatt atccaaaaaa ctctgatttc tttgagcttt tcacgcatta
     3901 ggaaacacct tgttatatct tttttgtatt tctttcaatt gttttttaaa actcataaac
     3961 aactgttcat gatctggtct aagcgttaga atatctaaat tatttatctt ttttaattgt
     4021 gtaataaaat ctattttgga ttttctttca atatcaaaac cgacaataat ctttcctgtt
     4081 ggatcaacgc atttcacttg aataactttg tcattttgcg atagctctaa tattgatgat
     4141 ttttcttttg cctcttccaa aagatgttct aacacttcgt taggtgttcc atgatagtaa
     4201 taaatcatgg tcttctcgaa aatttttatc ggatcaacac catattcttt tccgattcga
     4261 taaaaaaatt tcattggaat ttcttccgga tttctgttcc tgcttgacca gttagagatg
     4321 gtttgataat ttgcaaaatg gattcgttcc gctaatctct gaactgtggt ctctaaatct
     4381 tcttctatta attgttttac agcactaatg ttttgtgaca tttgctttct atcccttcaa
     4441 attcgttgca ttatcaattc taagatttca tgttttagtg catttctatc tacattttgc
     4501 ttttttaact ctgcaccata atatcttcgc aatcccttat ccaagcatac aattccttga
     4561 tctaatagat catattccgc tttttcaaac cttcgctttg cctgctcaac aagcaaaatg
     4621 atattgtgat gtatctgatt aaactgccga tcatattcct ctaaagtttt agcatctaag
     4681 ttagcaatat gatcctctgc taatttttgt gttaaaaata attcgacttt ctcagatttc
     4741 aacatctgta ttttttcata ctcttgtttt gcttggatat agtcatcttt caagctagac
     4801 atctaactca tctccttgcc tgtataaact gatacggact gtccaaaatt gaataggttg
     4861 gtgatgactt caagaagact tcaccttttt cacatgaaaa taaaccaatt aattcttcaa
     4921 tttcatttac gatcagatat ttattagtta aaattttccc attttctttt tctagctcga
     4981 caatcgcttg attccatgta ttttgcacta aatcttgaat caatttcaat ctatccactt
     5041 ttttcatctc atacagagca atttcagctt gtttaatgcc ttttaatttg cgaaaggcat
     5101 atttgtattg tgtgtaggtt aaaaagcctg tgtagttata catgggcttt cctgatgtct
     5161 tgtagtcatt taagcttgga tatatttcag ttttccattt ttcttctgaa cgttgatcaa
     5221 taattcttaa tttttcaggt aaatcaattt catagtattc ttcaattttc agctcacgat
     5281 aaagtagatg tttttccttt tctttttcaa ttacaaaaga attatttccc ttcagtttag
     5341 ctatttcttc acttgtgaga ccaagcctaa ttttttctac ttcacttcta agttcacgaa
     5401 tacttgttgc ctttaaccac ttcccatttg caccattatt gtaaccggta agtaactgta
     5461 ttttataaat tccttcacca tgaagcacct tatatatacc aacctgattc ttttgcggaa
     5521 tatgagcttc atttgcactg atttctttca ttacgcattc tcctttataa tctagttaat
     5581 tagcttgtgt gtttcgagta gttgagtaag aacgtttgtt tcagaaatca tttatttgtt
     5641 atcacttaat tttaaaaaaa tagggcttac gccctaaaga tctatttaac tttggattga
     5701 tcgtccataa caggctctga ttgttgatat aatttatatg ctcccaaatc catataatat
     5761 ttagttgtac tttcaatcat ttcatcagtt gtatttgttg tcattcccat tacatcccag
     5821 tatagtgtgc cgttaacttt ctttggtgtt actactgcaa ctgttggcgt atgatattga
     5881 ttgttctcat cagccagacc aggaatgttt tgtacccact caggtacacc atcagtaata
     5941 tctacagcaa ctgcgtaata ctcgtaagct ggtttattta tggtaatatc atgcattaat
     6001 gttggagcat aactaaatcc ttcttttatt tcttgcatga tttttgattc tgctttttga
     6061 caatctggac aatcaggttt gtaaagaacg atagtcagat ttttattttg caatggatca
     6121 ttcccataat tttcatcatt ttttactgta ttaataaagt tgttataaac ttcatccggc
     6181 ttttgacctt tattaatgtc ttcgtatttt tgatactttt cagcattatt ttcagtcatt
     6241 gaagacattg ttgttgatgc cgatgtagtg ctgtttttag taaccatcgt actactagac
     6301 tttgtgtctt tgttattatt a
//
LOCUS       DACDIE010000104         6173 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.107, whole genome shotgun sequence.
ACCESSION   DACDIE010000104 DACDIE010000000
VERSION     DACDIE010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.107"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            725..811
                     /locus_tag="IV250_002620"
     CDS             725..811
                     /locus_tag="IV250_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAP8769855.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="IV250_002621"
     CDS             complement(932..1621)
                     /locus_tag="IV250_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769856.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1866..2780)
                     /locus_tag="IV250_002622"
     CDS             complement(1866..2780)
                     /locus_tag="IV250_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP8769857.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="IV250_002623"
     CDS             complement(2762..3142)
                     /locus_tag="IV250_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP8769858.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="IV250_002624"
     CDS             complement(3592..3879)
                     /locus_tag="IV250_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP8769859.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="IV250_002625"
     CDS             complement(3899..4123)
                     /locus_tag="IV250_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HAP8769860.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4763..5296)
                     /locus_tag="IV250_002626"
     CDS             complement(4763..5296)
                     /locus_tag="IV250_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP8769861.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..6159)
                     /locus_tag="IV250_002627"
     CDS             complement(5317..6159)
                     /locus_tag="IV250_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAP8769862.1"
                     /translation="MREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEKILSL
                     RFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQF
                     NELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQEL
                     TAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALALADKQ
                     KEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLLSDLD
                     NLFQ"
BASE COUNT         1633 a         1151 c         1042 g         2347 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DACDIE010000105         6168 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.108, whole genome shotgun sequence.
ACCESSION   DACDIE010000105 DACDIE010000000
VERSION     DACDIE010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6168
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.108"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(489..863)
                     /locus_tag="IV250_002628"
     CDS             complement(489..863)
                     /locus_tag="IV250_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP8769863.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
     gene            complement(886..2286)
                     /locus_tag="IV250_002629"
     CDS             complement(886..2286)
                     /locus_tag="IV250_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HAP8769864.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            complement(2290..2700)
                     /locus_tag="IV250_002630"
     CDS             complement(2290..2700)
                     /locus_tag="IV250_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8769865.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            complement(2718..3209)
                     /locus_tag="IV250_002631"
     CDS             complement(2718..3209)
                     /locus_tag="IV250_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8769866.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            complement(3219..4010)
                     /locus_tag="IV250_002632"
     CDS             complement(3219..4010)
                     /locus_tag="IV250_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8769867.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            complement(4003..4776)
                     /locus_tag="IV250_002633"
     CDS             complement(4003..4776)
                     /locus_tag="IV250_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8769868.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            complement(4932..5966)
                     /locus_tag="IV250_002634"
     CDS             complement(4932..5966)
                     /locus_tag="IV250_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8769869.1"
                     /translation="MHGKITASAIARETNKLLNTYVYEAFDMPIEGETSDLINLINNF
                     CKSIDTTNGLILLVDMGSLEQMYEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPI
                     SHFYQMDFDSFQVKVQYYKGLSQKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIF
                     DFNALKKIAKEKDTMIFKNTICVLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDS
                     DSCEFCLNELVKLFTIEGASTKLRFLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINL
                     FLHLSGMIERIMIGDGITNEHLKEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYEL
                     IYTIFEQTIF"
BASE COUNT         1970 a         1228 c          805 g         2165 t
ORIGIN      
        1 agaaaacctc cagacttctt gatagctcaa gtatatcttg aggtgcattt gttagtaagg
       61 tactgttcta tggggcgctt actctaggag tgagcatttt tcgaaaaata ctaaaaattt
      121 ttcaaaataa aaaaaggcat acagacaata aactgtctat acgcctcaca gactctattc
      181 tatacatcta attttggctg tcctgcaaaa aatatgtgtt agcctccatc gaaagatcaa
      241 actttggaaa acatcattat aactttgctt ggtaagagct ttgctaaaac atgatatgac
      301 ttgtatacaa aatgtggagt gtattccaat aaataggaag gattacaaaa tttgaaacca
      361 aataagtaaa aaatactgtc actttgaatt aatttgaaat agtaaattct cattaaacta
      421 ttgaaatgct gataccaaaa aatgaagtac ttttcgttca taccaactta gagcacttcg
      481 ttatctattt atttcttcat ttggcttatc tctataatct catgatttgg tccgtagaaa
      541 ttaaagtaac ggattccatt ttcccagaac ggtaattcca taatattttt atctaataat
      601 tcatatccta aatcttgaat tgaattgaat acctcatcta tgtcatccgt atctaatgct
      661 agatgatcaa tggctcctct acactcagta gttttatctt cccatatttc tattatcgtt
      721 ccattcaact cgaaaaagcc gactttactt cccacattca tttcttcatt aatcagacta
      781 aaccctaagg attcataaaa gatttttgaa gctccaaaat cttttgttgg aattccaata
      841 tgttgtaaac catttaattt cacttacatc atcccttcaa ttgttttaaa atccacattc
      901 ataatttatt gaatagtcta gtttatctac cttgattgct ccctccgtaa cagtcatact
      961 taatgctgtt ccgactttat catacattct agtacttaaa gcaacattat caatatataa
     1021 aacagctatc gtatcatcaa caattaactt tagttcatat tctttgccta tttccaaaaa
     1081 tagtggtcgt tgcagcccct tatcaaacat ttgaggccaa ggatagttcg gagtttttcc
     1141 gaaagtaagc tgattattaa tcaatgaaat acgaaattcg tatccttctt gagtatcact
     1201 atctacaaat aattttaaag caaactcctt tgttaacgat gtaatagtca aagttgtttc
     1261 aaatagaaaa taatcttcga ttttaggaac aattactctt tctttttttc caaattcttt
     1321 ctctaactta aaacttgaaa atggagtgat ttctttaaaa ttctcaatta ttttttcagg
     1381 cagtttaaca ccaagagttt tgtctgtctt ttgaataatt tgatgtggaa caagtgcacc
     1441 gccccaatcc cagttatttt gatcgttctc cccttctttt gttgccaccc agccagttag
     1501 aaaacggcca gtttcattag ttccgactgt tcttgctgca taataggctt ttccgtcgaa
     1561 agcttcgtct actggtgatt tccaatctga aaataatcct gctccgactc gatatttagt
     1621 ctttttatct tcacaatact ctgaaaaaag taaataccac gttccatcaa tttcaaatat
     1681 atcaggcatt tcaatcatgt taaattcatt attagcccag tagtctcctt tgaaattcca
     1741 atcttttaaa tcttcggatt caaaatagac caatctcccc gtcggctttt tcttacttgc
     1801 tggttttcta gctcctagta tcaaaacgta ttttccatga tcttgatcaa aaattgcaaa
     1861 aggatcgcgc caatcattct tatcatatcc ttcttgagga acaagttttg ctgcttcgcc
     1921 atcctttttc caatgaatta aatctttaga acttgcaatc attaacactt ctgaagtttt
     1981 tccatccaac ttagctttgc gattatgtcc agtatacaaa gctaaatact cattgattcc
     2041 ttggacaata cttccagcgt aaatgtattg atcaatgtca tcgttgccac cacgatgaat
     2101 tacttccccg tattcttcaa atttcacaaa atcagtggtt gtaactagtg accacccaaa
     2161 aggatcagtt aaaggtttag gatttctcgt atctcgttga tgaaatagat aaaaagttcc
     2221 atctttataa aaaggcatgc aatccccaaa ccaaaaatct ttaggcttgt aaaaaatttt
     2281 ttgcatttat cactcatcct cttcgttagt atgtctttgc atatctgcaa gacacgcaat
     2341 tgtattcata tttcttagat tttcgatgat atcatcgctt ttttttcctt gtaatctcaa
     2401 attgacagcc tcaatcaaaa ttcctaaatt catgccacca agaagttgaa acccattttg
     2461 aaatacttca cttcttttag caaattcgtt gaaaggtgtt ccaccaatca agtcagcaaa
     2521 taccataact tcttgatttt ttacttgaga aactattttt tcaatgtttt cacggaattc
     2581 ttcaatgcct gcatctccaa gtgttaccac atgacaattt aaattatcgc caaaaaacat
     2641 ttttaatgta tctacaaatc caatggcaat atttccatga gatgtaatta taaactccat
     2701 ccactcatcc tctgttctta ataaataaaa tcctttaaaa attcttttga atctctagga
     2761 gtttgtatca ctgtcacttc aactccatca ctaattagtt ctttaaaagc ctctctctct
     2821 tcttcagtaa tatttatcgt cggacggatg cgttctgttc cattccttct tgataaattt
     2881 cctacgttta cacttgttat tggtaaaccg ttatcaatca aaaatttaac atcgattgga
     2941 gaaactgcaa caataagaac tttttgtcct tcatatttac cagaaagaat atttttaact
     3001 gctacttccc ttgtaattat tgaagttcta acattaggtg gtgcagcgag gcgaactaca
     3061 gcttttttta cgtcatcatt ggctacttca ttattaataa ccataattct gttagcactc
     3121 aattgatttg tccattgtgt tgctacttgt ccatgaatca ttcgatcatc aatacgaaca
     3181 tgtataatac ctttcatttt ttcatccata ttcaacacct acttcaaaat atttaacgct
     3241 cttaacgcaa tagaaactac taagattaat agaattacat aaactggttt aacctttcgt
     3301 cctaacaaat aatacacaat tgctactaca catgcaggta ataaaccagg cataacttga
     3361 tcaaagattt catttccggt cattgttaag tcgccttgtt taaattcaaa gccaacattc
     3421 acttttacaa ctgtggcaat gagtgcaccg accaccatta accctaaaat tgatactgca
     3481 tcagtaatag actttaattt tgtttcaatt tgagttagta ggctcgcacc cgattctttc
     3541 ccagcaaggt ataaaggata agcagtaatc cttaataaaa ttgatgccag tatccacaaa
     3601 ataattccaa caaaactacc ttccaatgcc atattagctg caattgctcc aaaaatagtc
     3661 catggaataa ctacaaatac actatctcca attcccgcta aaggacccat tagacctgtt
     3721 ttcagagcac ttactgcttc caaagactta tttttttctt ctttttcaat tccaacattc
     3781 ataccaataa tgtaaggcgc tgtatacggc gtagtgttaa aaaattgtag gtgagctaaa
     3841 acggattgtt tcattccttc aggatcatct ttatatgtgt ccttgataaa aggaagcata
     3901 gaatacgcat aacccaaccc ttccattttt tcataattta atgaagttgc accattgaga
     3961 atccaccgga aaattacttg tttgtcgatt tttgtcagtt tcttattcat cttcgtcaaa
     4021 acctcctgtt gcagctggtg ctacttgagt attatttgat ctatttttat atacgattag
     4081 tgcgattgca gcgccaagta acgaaatccc taaaattggt atttggaaat aaactgccaa
     4141 aacgaaacca actaacaaat aggcaaagta atcttttgta ggtaaatatt taagtagcat
     4201 tcctatccca acagcaggta atagtccccc tgctaccttt aaaccaccgg taacccattc
     4261 aggaatataa ttgattgcac ttcccactaa cgttgggcct aatatcacag ttaacaaaac
     4321 aggaattcca gatacaaagc ttgtattaat tgatgccaaa tattgcatcc actctaattt
     4381 ttgataatct tctttttgtg cgaatttttc tgccttttgt tgaaaaaaaa tgtttgttga
     4441 ccactttaga acatctagtt gtacaattaa cattgctacc ggtattgcta aagttattcc
     4501 tatagaagca tcctggttag ttgagattgc taaataagta ccgatcaatg ctgctgtttg
     4561 ataatccggt actgatgcac caccaaaact agtaatacct agtgtcatta attgcaaagt
     4621 cccaccgata taaagtccag tttgaatatc tcccataata agtccggtca agaaacctgc
     4681 aacgacaggt tgggaggttc cgattttggg cccttcctta tcccagttaa tgaaaaatcc
     4741 atacaacaca actaaaaatg tttgccaaaa cgccattttg ttctctcctt tcttcttata
     4801 cttttatata agcaattact gtgccaacta acattatcca ataaagcggg aaaatacagt
     4861 aaatattaat taaatgagcg tctaaatata ctttggacac cctttcattt ttttagacgc
     4921 tagtatgcta tttagaaaat agtctgctca aaaattgtat agattaactc atactctcct
     4981 ttgggtattt caatacagta aatatctttg atttctgaaa aaaaatcttt tgatacattt
     5041 aaaaaagtat caaatagttg cgctccctct ttcaaatgtt catttgttat tccatcacca
     5101 atcataattc gttcaatcat tccagaaaga tgtagaaata gattaattct cagaaaactt
     5161 gggattttga catcgtaata tttttcatac aaatatagta ctttttctat ctcattgaat
     5221 acttttttgg aatctaaaaa gcgaagctta gtactagcgc cttcaatagt aaagagttta
     5281 actaattcat ttaagcagaa ctcacagcta tccgagtcca tatacttatt taatttttct
     5341 aaggtttgat tcccattaat tagattttct aaatttaaac aatcgattcc ttgaatgtaa
     5401 aattcagtgg ttgaaagaac acaaattgta ttcttaaaaa tcatagtatc tttttccttt
     5461 gctattttct ttaatgcgtt aaaatcaaaa atcaaaatct ccacttgatt atcgagatac
     5521 cgttttaaaa tatccttaac tttttcagaa atcccctcac cagagagaca agatactatt
     5581 acatttttct tttgtgataa tcctttataa tactgaactt taacttgaaa cgaatcaaaa
     5641 tccatctggt aaaaatgact aattggcttt ttttggcaaa cttgaatccc acattcaatc
     5701 gctaaagctg tagaaacatt gtttaaaatt accaaatcac ctattacatt ggattcaatt
     5761 ttttcataca tttgttccaa tgatcccata tcaactagca gaatgagtcc attcgtcgta
     5821 tctatacttt tacaaaaatt gttgattagg ttaattaagt cactagtctc accttcaatt
     5881 ggcatatcga aagcctcata aacatatgta ttcagcaact tatttgtttc ccttgcgatc
     5941 gcagaagctg ttattttacc gtgcataact atcagacaat gggtttctgg tattttcaca
     6001 tagaacttct ttacaagaaa aagtatccat ttcaacaaaa gttcttgttt actttcttcg
     6061 acttctaaac tactccaaat ctcatttgcc aataattttt gtttatcatt tttcatacga
     6121 aattttctga acttagacag caaaaaattt cttgatgttt cttcaatt
//
LOCUS       DACDIE010000106         6045 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.109, whole genome shotgun sequence.
ACCESSION   DACDIE010000106 DACDIE010000000
VERSION     DACDIE010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6045
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.109"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(117..1079)
                     /locus_tag="IV250_002635"
     CDS             complement(117..1079)
                     /locus_tag="IV250_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8769870.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1081..2520)
                     /locus_tag="IV250_002636"
     CDS             complement(1081..2520)
                     /locus_tag="IV250_002636"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAP8769871.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2705..4651
                     /locus_tag="IV250_002637"
     CDS             2705..4651
                     /locus_tag="IV250_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HAP8769872.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            4915..5787
                     /locus_tag="IV250_002638"
     CDS             4915..5787
                     /locus_tag="IV250_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8769873.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIENRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT         1910 a         1038 c         1069 g         2028 t
ORIGIN      
        1 tctaactttt gggggtcact acaagttgac aactcctttt tgtttacaaa tcaattggat
       61 atctacaaat tgtattttct aacgaatttt atagacatta aaaaattaat ttaacgttaa
      121 atacttcctc cagaaagtat tgagattggt aaaatataat ccttatttgt cttttcgcct
      181 ttaatttttt tcagaagaat atcaacaata agacttgcaa tttcatcaat tggttgtctt
      241 attgttgtca attgtgggaa aaatttctca ataaaagagg taccatcgta tccaataatt
      301 tttaaatctt ctgggatatt aagtttaagt tgatgagcaa ttttcatagt gagaatggct
      361 gttaaatcat cagaaacaaa aataccatct ggtttgtgat ttataatagt tgactttatt
      421 tccatttcac gacggatagt agacagatta ttaggaattt tgaaaatttt tccatttgga
      481 ttttgaaaag aaaaacctag tgcacgtaat tctgtgggag aatccgtgtt atcatttcct
      541 gtaatcataa tggtattttg acaaccattt ttttgtaaag tcttagctgc aagttttccc
      601 ccttcaaagt tatctgaaga aacaatggaa acattaggag ctaaatttct atcaaaagca
      661 acaataggtg ctctcacacg ctcataatca gcaattccaa gattatggct tgatgagatt
      721 atgccatcaa cttggttggc ttcaagcatt tcaatgtatt ctctctcttt agcaggatca
      781 ttttgactat tgcaaataat cgttttatat ccatgtttaa ataactctat ttcgagatac
      841 tcaatgagtt cagagtaaaa gatattacta atattaggaa aaataagacc aataagctga
      901 ggtgactttc cttgaagtcc acgtgctaaa ttatttggtt tataaccaag agttttcata
      961 gcttcatgaa ctttttgttt tgttttttct gataaatatc ccttattatt aatgacgcgt
     1021 gaaacggttg ttgggctaac tccagctaat tcagcgacat cagttaattt tgctaccatt
     1081 ttaatatttt aattcaaagt attttccagt aggttcgcct gatttgattt gaataccatt
     1141 ttggtgattt tcagggaata cacgactagt aaacactttt tctcctttat taataaaaat
     1201 ctcaatgatt gagttatcta caaaaatgtt agcagttgtt tctttagtat caattgagca
     1261 ttcacgaatc gtaccaaagt cagtggcata ttgaatgcct gctttgctgc gatctaaaat
     1321 aatttttcct tctttggtat ctacaatcaa gctaagtcca tttcctttac tatcagagaa
     1381 aagaagtaac tccgatttct gatttggagg aaaagttagt tcaagctcat aagtattatt
     1441 tgtttctgct tttgctgtaa attgttcaga actagcacgt aatgatgtaa tagcttctac
     1501 tggatattgg taaagtttac catcttttat tgaaagttct tttacaagac tcattgctcc
     1561 ctgataatca tacttatcag ttggataatc gatatcaggt agtccaatcc aactgacaat
     1621 cagagcacga ccatcaggag tattgaatcc ttgagtcgca tacgcttcaa atccataatc
     1681 aagattttga atttctgaag cgcccacaag ttttactttt tctatgtcaa gcgattgaca
     1741 gatcttaaaa gtattcggat agatattccc ataatttagc tcagatttat ccaacccttg
     1801 aggacaataa atgagaacag gttttttgtc tacaaatact agatttggac actcaatcat
     1861 gtattctgaa ccagtgccgc caaaatcaag gttgccaact tcctcccatg tttccacgtt
     1921 attgtcatcc gctttgtaaa gtttgataaa gcctgatttt tctaaatttt gtgctccaac
     1981 aatcgcatac aattgtcctt cataattaaa catttgtgga tcacgaaaat gatcagttac
     2041 atcagcaggt ttatgaataa gaacattctc acctttcgta atgttgtagt ctttatccat
     2101 ccatgctccg atttgaagtg tatcacgttc ccaattttca tttcgaacgt ttcctgtata
     2161 gagtaagaat agattcccct ctatttcata tgcagaaccg gaataagcgc cgtggctatc
     2221 tcgttttgtg tctggaagaa gttctgctcc tgttacatga aaatgaacta aatcatctga
     2281 ttcattgtgt acccattctt tcaacccatg agcagctcca aatggccaat tttgataaaa
     2341 aagcgtatat tttccgttaa aaaaagaaaa accattagga tcgtttaaca atcctgtttt
     2401 aggttcgata tgatagctag cacgccaagg tgatcgtttt acattatcct taattttatc
     2461 catctcatct ttagtccaat cagaataagc acgatatcgt atttctcgag gtaagttcat
     2521 attttactcc ttcaatttat ctttttacaa ctagtatggt aaacgttttc cgttgaaaaa
     2581 tcaatcattt catttaaaaa aataaactat atgacaaacg tttgacatat ttttaagata
     2641 tgctaaaata aatttgcatt tcagtgaacc gttttcacgt aaaaataaaa aaggagtgat
     2701 tcgaatgaat tacagtaaag tagcttccga cgtaatacaa gcggtcggaa aagataacct
     2761 tattgcagct gcacactgcg ctacacgtct tcgtctcgta ctaaaagatg acacgaaagt
     2821 gaatcaaaaa gctttagacg aaaatccaga tgtgaaaagc acatttaaaa ttgatggaca
     2881 atatcaagtg attatcggtg caggtgatgt taatttcgtt tatgacgaac ttatcaaaaa
     2941 aactggactt tcggaaattt caacagatga tttaaaacaa attgctagta aagataaaaa
     3001 attcaacccc attatggcat taattaaatt attatcagat atttttgttc caattatacc
     3061 tgcccttgtt gcaggtggtt tattaatggc attacaaaac tttttaacag ctgctggact
     3121 ttttggacca aaatcaattg aagaaatgta tcctgcgatt gaaggtattt cctcaatgat
     3181 ccaactgatg tccgctgcac cattcatgtt cttaccaatt ttagtaggta tttcagctgc
     3241 taaacgtttt ggagcaaacc aatttcttgg tgctgcaatt ggtatgatca tgacaacacc
     3301 agatcttggt ggctcaactg aatattggaa tatttttggt tatcatgttt cacaaacaaa
     3361 ctatgcttat caagttattc cagttttagc agctgtttgg atattatcta tattagaaaa
     3421 attctttcat aaaaaattgc catcatctat tgactttaca ttcacgccat tattatcagt
     3481 aatgattact ggatttttga ctttcacagt tattggccct gtaatgttat tgttgtctaa
     3541 tggaattaca gacattattg tttggctata taacactact ggatttattg gaatgggtat
     3601 ttttggagga acatattcac ttatagtcat gactggttta catcaatcat ttccagctat
     3661 tgaaactcaa ttactttcag cgtggacaaa tggtatcgga catggagact ttatctttgt
     3721 agttgcatct atggcaaacg ttgctcaagg tgctgcaaca tttgccattt ggttcttaac
     3781 taaaaattct aagacaaaaa gtcttgcttc ctctgcgggt gtctcagcac ttcttggaat
     3841 tacagagcct gccttgttcg gagttaactt aaaataccgc tttccattct tttgtgcttt
     3901 aattggttca ggaattgcta cagcagtagc aggattactt aaagtagttg ctgtttcact
     3961 tggttcagca ggtttcttgg gatttctttc aattaatgca acatctattc cattttacgt
     4021 attgtgtgaa ttgattagct ttgtagttgc ttttgcaatt acttacttct acggtaaaac
     4081 aaaagctgct ggtatcttta aagcagaaga aattgctgaa caaacaccag ttaattcttc
     4141 aaaagtagca acaacagatc caatatctac tgatgttgaa tcaacacaaa caaaagtttc
     4201 atcagaaaca attgttagtc ccatttctgg taaagtgatt agtctttctt cagtaaatga
     4261 tccagtcttt tcatcagaag ctatgggaaa agggattgcc attaaaccaa atggaaacac
     4321 ggtccattct ccagtagatg gtactattca actcacgttt gaaactggac atgcttatgg
     4381 tattaaatca aataatggtg ctgaagttct aattcatatt ggtattgata ctgtatcaat
     4441 gggaggaaaa ggatttacac aaaaagttac tactaatcaa aaagttagaa aaggcgatat
     4501 tcttggagca tttgaccgtt caatgattga gaaagaaggt cttgatgata caacaatgat
     4561 tattgtgacc aatacttcgg attattcaga agtaaatata gtagctgaaa atacagtcac
     4621 tgagggtact gatttacttg aaatcaaata aatatatttc cctgagtaaa gatggaattt
     4681 tctttccaaa atcaaataat atagattaat caaatatttg agaataacat tttatttaat
     4741 cttttcatat atcaattgta tgaaaaatta ttagaatttt aaaaaatgat aaaaattcaa
     4801 aaagaaaaaa tagtagttat ttacttgtca tgttactaaa taaaaaaata atttaaatat
     4861 gatatatagt catttattta gtgatttcat tctaaaataa agggagaaat gtctatgcta
     4921 tacggtgcaa ttgaagcagg cggcacaaaa tttgtgtgtg ccattggcga tgaagaaatg
     4981 acgatcaagg aaagagtgag ctttccgaca acgacacctg aggaaacaat gcctttagtc
     5041 attgacttct tcaaacaata ccaagcagat ttagcaggga ttgggattgg ctcatttggg
     5101 cctatcgata tccatcgcga ttccgccact tatgggtaca ttacctcgac cccgaaattg
     5161 gcatggcaaa attttgattt catcggtacc atgaaaaaag aatttccgat cccgatttct
     5221 tggacaacag atgtcaatgc ggctgcctat ggtgagtatg tcttcggcag tggcaaagga
     5281 ttgagcagtg tcgtttacta tacgatcggt acgggtgttg gcggcggtgc cttacaagaa
     5341 gggcgcttta tcgaagggtt tagtcatcca gaaatggggc atatgctcgt cgtccctcat
     5401 ccaaaagatg actttgctgg cagctgtcca tttcatggca actgtttaga agggatggcc
     5461 gctggacccg ccatcgaaaa ccgcttaggg aaaaaaggac aagaggttgc agaagatgat
     5521 ccttattggg aaatcgaagc ctcctatatt gctcaatgtg cctacaatac aaccttgatg
     5581 ttttctccag atgtgatcat ttttggcggt ggtgtgatga aacaacgcca tatgctgcaa
     5641 aacgtccacg acgcatttgc aaaattggtc aatgggtatg tcgaaacacc tgagttaagc
     5701 aaatatatcg tgaccccagc attagaagac aacgctggaa cgctcggttg tttggcattg
     5761 gctcgagaag cggtcttaca ttcataaacg aaaaaaggct cttcgacatt tagtgttggt
     5821 agagaaagat acaactgaaa atttagtcat aaccagttag gcagagtgct taactggttt
     5881 ttgttttact tggaagatca agccttgaat gagataaatt cttgctcgaa agttatggaa
     5941 atttcgatag ccgtaggcca cttgtattgt gcaatagttt tttacaaaaa aaagggggtg
     6001 cggatatttt tttggacctt tttaacaaat atatctttgc ttctt
//
LOCUS       DACDIE010000107         5916 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.110, whole genome shotgun sequence.
ACCESSION   DACDIE010000107 DACDIE010000000
VERSION     DACDIE010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5916
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.110"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..482
                     /locus_tag="IV250_002639"
     CDS             <1..482
                     /locus_tag="IV250_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8769874.1"
                     /translation="SIRKLNDAQVLELQTLKPNDLVVIRLELNGKKVSLSNASAGQKT
                     SAILSMILAYGNSPLILDQPEDDLDSQLINNLIVRSIITKKENRQILIVTHNANIPVN
                     GDSEWLISMGDSKEISADQCGSVDEPNIKPRICTVMEGGEDAFTNRAKRYGFKQIVE"
     gene            532..921
                     /locus_tag="IV250_002640"
     CDS             532..921
                     /locus_tag="IV250_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8769875.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            908..2410
                     /locus_tag="IV250_002641"
                     /pseudo
     CDS             908..2410
                     /locus_tag="IV250_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
     gene            3155..4981
                     /gene="ltrA"
                     /locus_tag="IV250_002642"
     CDS             3155..4981
                     /gene="ltrA"
                     /locus_tag="IV250_002642"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP8769876.1"
                     /translation="MLKKNKLRYNEYYDMQHIYDELYAQSKNGNNFYKLLEIIGSEQN
                     ICLAYRNLKSNSGSKTAGTDGMTIDDIKQLSNAEIVATVRESLSNYRPKSVRRVFIPK
                     AGSDKMRPLGIPCIWDRLVQQCILQVLEPICEPKFHNHSYGFRANRSAHHAVSRVTTL
                     INLSKYHYCVDVDIKGFFDNVNHGKLLKQIWTLGIRDKRLICIISKMLKAEIDGEGVP
                     EKGTPQGGLLSPLLSLIVLNELDWWVSSQWETFQPKNRSKNGWLQYAKKYTKLKSGFI
                     VRYADDFKIMCSTYGEAQRFYHSTVDFLNKRLKLEISPEKSKVVNLKKNSSDFLGFKI
                     KVIPKGRTKHGYVAKTDMNQKALKKAKTNLKLKVKDIARHTTGFNISRYNLTVIGMQN
                     YYCIATNVYNNLTEVSYALLPTIRIRLRNIAKSVPFESTSSEFQSRTKGIRPKTKIVM
                     IADNPLLPIQGVQHKNPMNFSQDICNFTKQGRNKVHEDVVVVTKEEIRALLENENPAD
                     SVEFNDNRISAYIAQQGNCYVMNRRGTPSTLICIHKSDKGDNLDRYSNLAFVEIPIAK
                     AILTESADEAKSLLKGYVLNSQQKKKLNRIRTNYGYQTITGK"
     gene            5115..>5916
                     /locus_tag="IV250_002643"
     CDS             5115..>5916
                     /locus_tag="IV250_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8769877.1"
                     /translation="MPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILT
                     FLTGISSRDGEKFPVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTD
                     YDFAHLLFSDGSITQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVIS
                     TFALDFIHSNREIFKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDD
                     LLDERLKNNIGLKFAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT         2002 a         1013 c         1289 g         1612 t
ORIGIN      
        1 attctataag aaaattgaat gatgcacaag ttcttgaatt acaaaccctt aagccaaatg
       61 atttagtagt tatccgactt gaactaaatg gaaaaaaagt tagtttatcg aatgctagtg
      121 ctggtcaaaa gacatctgca attctttcta tgattttggc gtatggaaat tcaccactca
      181 tattggatca gcctgaagat gatttggata gtcaattaat taataactta atcgtaagaa
      241 gtatcattac aaaaaaggaa aacagacaaa ttttaattgt aactcataat gcaaatatcc
      301 cagttaatgg tgattcagaa tggttgatta gcatgggaga ttcaaaagaa atatcggctg
      361 atcagtgcgg gtcggtagat gaacccaata tcaagccaag aatatgtact gtgatggaag
      421 gtggcgagga tgcttttaca aatagagcga aacgctatgg ttttaaacaa attgtagaat
      481 aacactagag ggtgagacat attattatgt ccgccctttt taggaggaaa aatgaaaaaa
      541 ataaaaagct ataccagtat ctgggcagtt gaaaaagtca tttacgcaat caatgatttt
      601 cagttgcctt ttcctgtgac ttttaaccaa atggcttggt tcgttttatc tttactgttt
      661 gtcatcgtat ttgcccatgt gcctccacta tctatgatag aggaggtctt tttaaaatac
      721 ttaggcatac cggtggctgt cacttggttt atgtcgcaaa agacgtttga tggcaagaaa
      781 ccactgggat ttttacgatc ctttatcagc tatcacttgc gatttaaggt gacttttgca
      841 ggaaagaaag tcaaagaaca gaagaaacga tgggatgaac ccagtactct tgtaaggagt
      901 gtgaactatg tatccgatta agtatattga aaataatctc gtctttaacc aagaaggcga
      961 gtgttttgcc tactatgaat tagtgcctta caattactcc tttttgtcac ccgaacagaa
     1021 atttcaagtg catgataact ttcgccaact aattgcgcaa aatcgtgaag gcaagatcca
     1081 tgcgttacaa attgccacag aaagtagtat tcgcgccaca caagaacgat caaaaaaaga
     1141 aattaccggt agactcaaag aagtagcgaa acaacgaatt gatatacaga cagatgcatt
     1201 agtatccatg attggcgata gtcagattga ttatcgcttt tttattggct ttaaactaat
     1261 tgcgacagat gaagaagtca atctaaagag tctcaagaaa tctttctttt ctgggcttca
     1321 agaatttgtc tatggagtga atcatcattt gatgggcgat tttgtttcct tatccaatga
     1381 agaaattcgc tgctattcta agcttgaaaa attaatggaa agcaaattgg ctcgaagatt
     1441 taaagtcagg cgagtcacac cgagtgacct gacgtatttg attgaacata tttatggcga
     1501 aaaagggata ccgtttgaag aatacgaatt tcagctacca aagaaaaagc taaaatcaga
     1561 aacactggtg aaacgttatg acttattacg tccaaatcgt tgtttaattg aagaaaaacc
     1621 aaggtgctta cggatggaac atgagaatca tgagtcgtac gtagcgtatt taacaattaa
     1681 tacaattgtg ggggagatgg aatttccttc ctcagaactt ttctattacc agcaacaaca
     1741 atttacattc ccaatcgaca caagtatgaa tgtagagatt gtgacgaata aaaaggcttt
     1801 agctaccgtt cgtaataaga aaaaagaact gaaagattta gacaatcatg cttaccaatc
     1861 tgacaatgaa acgaattcca atgtcttaga tgctttggat tccgtggatg aattggaaac
     1921 caccttggat cagtcaaaag aatctatgta caaactgagt tatgtggttc gagtgagtgc
     1981 cgaatcagtg gatgaactaa agcgacgctg tgatgaagtg ctcgattttt atgatgatac
     2041 caatgtaaaa ctagtccggc cctttggcga tatgatgggg ctacatgaag aattcttacc
     2101 cttaagtaaa cgatatatga acgactatat tcagtatgtt acgtctgatt ttttggcggg
     2161 gcttggtttt ggggctactc agatgctagg agaattagaa gggatttact ttggctacaa
     2221 tgtggataca ggtcgtaatg tttacctgaa accggcattg gcttcacaag gagtcaaggg
     2281 ttcagtaacc aatgcgctgg ccgccgcttt cctgggttct cttggtggag gtaaatcctt
     2341 tagtaacaat ctcttagtct actatgctgt tttgttcggt ggtcaagctg tgattgtcga
     2401 tccaaaaggg ggtgcgaaac gttcctactt gaaaagagta ggcacagcac ttcattaaat
     2461 caatatttaa tgtgtgttgg agaactaaac acacgaatag tcgaatgacg gggtaacgcc
     2521 ctgaaaggct actttaatac tctgactggc agaaacttga gtaaacaagg ctctgtcaga
     2581 agctcggtga agtcggcaga cagacaccgt aacagataat gctgacgaaa gattcccaga
     2641 ggtgggcgtt gtgtcctata tgccggaagt ctataaaata tctatggtta gaatgtagat
     2701 gagaggtcta ctgacgaatc cttgaatgta caggtctata tcggggctgg atagaaatgt
     2761 ccagtgacac aaacagtgtc gctactgtgg gtgagtaaga ctccgcattt atgaaaatcc
     2821 atataacgtt aaaggcgtta tcaaggtagc aggcttatag aggaaaccta aggatatatg
     2881 tacagatagt gtgtttggaa cgttgaaagc tgacaacatg gagattttac tgtctgtgaa
     2941 gtgttcacat cagaaagtta ttgtgagatt agcaatgata taaggtgtgt taatgtcagt
     3001 gagagtggtg gcacagtacc tatgaaaccg tgataacaag cggtggaggg atagccacta
     3061 gtcatatgaa gaatagaatt actaagaaaa atggtatggg ttcgagtaag actaagagat
     3121 gtacttgctc ctgcaaacaa ggagggtaca gaccatgcta aagaaaaaca agctccgcta
     3181 taacgagtat tatgatatgc aacatattta tgacgagtta tacgcacaga gcaagaacgg
     3241 taataacttt tataagttac ttgaaattat tggttcggaa caaaatattt gtttagctta
     3301 ccgtaatctg aaatcaaaca gtggcagtaa aacggctgga acggatggaa tgacgataga
     3361 cgatataaaa caactcagca atgctgagat tgttgctacc gttcgggaaa gccttagcaa
     3421 ctaccgcccc aaatcagtca gacgtgtttt cattccaaaa gctgggagcg ataaaatgcg
     3481 tccattgggg attccgtgta tctgggacag gctggtacaa caatgtatcc tgcaagtcct
     3541 agagccgata tgcgaaccaa agttccataa tcattcctat ggattccgtg cgaatcgaag
     3601 tgcccaccat gcagtcagca gagtaacgac actgataaat ttatcaaaat atcattactg
     3661 tgttgacgta gacattaaag gatttttcga taatgtcaat catggaaaac tattaaaaca
     3721 aatctggaca cttggtattc gggataaaag attgatttgc attatcagca aaatgctgaa
     3781 agccgaaatt gacggcgagg gtgtcccaga aaaaggaacg ccacaaggcg gtctgttaag
     3841 tccactgctc tccctcatag tccttaatga attagactgg tgggtaagca gtcagtggga
     3901 aacattccag ccgaagaacc gcagtaaaaa tgggtggctt caatacgcaa agaaatatac
     3961 caaactgaaa agcggtttta ttgtacgtta cgctgatgat tttaaaatca tgtgttcaac
     4021 ctatggagaa gcacaaagat tttaccacag taccgtagat tttcttaata aaaggctgaa
     4081 attagaaatc agtccagaga aatcaaaggt agtaaatctg aagaaaaatt catcagattt
     4141 tctggggttc aaaatcaaag tcattccaaa aggaagaacg aaacacggtt atgttgccaa
     4201 aacagatatg aaccaaaaag cattaaagaa agcgaaaaca aatctaaagt taaaggtaaa
     4261 agatatagcc agacatacga ctggcttcaa tatcagcaga tataatctga cagttatcgg
     4321 tatgcagaat tactactgca tagcaactaa cgtatataac aacctgacag aagtaagtta
     4381 cgctctctta ccgaccatac gaatccgttt aaggaacatt gcaaaatcag ttccatttga
     4441 aagtacaagt tcagaatttc agagcaggac aaaaggaatc agaccaaaaa caaaaatagt
     4501 gatgattgcg gataatccgc tattgccgat tcaaggagta caacacaaaa atcctatgaa
     4561 tttctcgcaa gatatttgta attttacaaa gcaagggaga aacaaggtgc atgaggacgt
     4621 tgtagttgta acaaaagaag aaatccgagc gttgttggaa aatgaaaacc ctgccgatag
     4681 cgtagagttt aacgacaatc gtatttcggc atatatcgca caacaaggca actgctatgt
     4741 aatgaatcgt cgaggaactc cgtccactct gatatgtatt cacaaatcag acaaaggaga
     4801 taaccttgac agatattcta atctggcatt tgtggaaatt cccattgcaa aggcaatcct
     4861 aacagaatca gcagacgaag caaaatcact gttaaaaggt tatgttctta atagtcagca
     4921 gaagaaaaaa ctgaacagaa tacggacgaa ttacggatac cagacaataa ctgggaaata
     4981 acttataaat ctatttggat atgatggaac gccgtatgaa gcgaaagttt catgtacggt
     5041 gtgaagcggg ggaaaacttg gagataacat caaaaagtta cctatcgcta tagcgaggag
     5101 ggtggaaaga aaccttgcca gaaattgcgg atgaaatcag tatcctcaac ttgacgagta
     5161 agtcagaaaa tcaaggtctg cttgatcctt atgtgattat gaagaaaaag aaagactcgg
     5221 aaagtttagc aattgatatt ctgacttttt taacaggaat ttcaagtcga gacggggaga
     5281 agtttcctgt gttacgtaaa gccattcgaa cggtgactca aagtgatcaa cgggggttat
     5341 tacaagttat taacgaattg cgaaaagacg ggtcaccaat cgcggaaaat atcgccgatc
     5401 atatcgagtc ctttaccgat tatgattttg ctcatttgct tttctcagac ggctcgatta
     5461 cccaatcgat tagtttagat aaacagctaa atatcattca agtggcggat ttggttttac
     5521 cggatgcaga aacgactttt gaagaatata ccaccatgga attgcttagt gtttcgatgc
     5581 taatcgtcat ttccactttt gccttggact ttatccatag taaccgagaa atcttcaaaa
     5641 ttgtcgattt ggatgaggct tggagttttc ttcaagtggc tcaaggaaaa acgttatcga
     5701 ataagcttgt tcgtgctggt cgggccatga atgctggggt ctatttcgtc actcaaaata
     5761 gcgacgattt attagatgaa cgtttaaaaa ataatatcgg gttgaagttt gcttttcgca
     5821 gtaccgatat tcatgaaatc aagaaaacct tggaattctt tggtttggat caggaggatc
     5881 aaagcaatca aaaacgcctg cgagatttgg aaaacg
//
LOCUS       DACDIE010000108         5751 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.111, whole genome shotgun sequence.
ACCESSION   DACDIE010000108 DACDIE010000000
VERSION     DACDIE010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5751
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.111"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..434
                     /locus_tag="IV250_002644"
     CDS             <1..434
                     /locus_tag="IV250_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAP8769878.1"
                     /translation="KDYWGESKGFIYPDSNMGGNWKATNPKKEQEAMKLKNTKSNNLL
                     YATCKHFRHVRDTEFTSYHLSGIVIDSFVYEAMGNWKFVENNSGGQNISSVSYETALL
                     EYYNSHKVMGGLNLYSPGSNQFVNSDSSIICLEKVLKKIAL"
     gene            454..1014
                     /locus_tag="IV250_002645"
     CDS             454..1014
                     /locus_tag="IV250_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLATT domain-containing protein"
                     /protein_id="HAP8769879.1"
                     /translation="MGDEKKRLEVQLREAYGRVTYTYTTHLKQADNIISDNKILRCLQ
                     IGLSALTTGGILSTIITDNSELTFVAGLCSTISLGLNLYFKNFDLAEEAKNHQIVADE
                     LWLIREEYVSLLTDLNRLSVEDIVKKRDQLMNETHEAYKNAPKTNSKSYESAQKALKS
                     EEEQFFTEEELDKMLPEHLRKKSSKY"
     gene            1838..2605
                     /locus_tag="IV250_002646"
     CDS             1838..2605
                     /locus_tag="IV250_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HAP8769880.1"
                     /translation="MRDRILEVNQEMVNKLTNISGNYEKYDGTLLDNYIFHDTQNIRI
                     GRSKPRAFILIREKYLNEWNSTYEMVMTDNFEKVLDFRNALLGSDMGNSSLESIEVEN
                     KTENEGEGLIKNVLKIEQKYISEDRSFYRVTSSVGLANFATNLIGNNASESLLVMCLN
                     TKNEVIAYSEVFKGGINQSVAIPREIFQLALLNNSAKIAVSHNHPSGYEFPSENDKSF
                     TTNLKNAGKTIGIELLDHIIVTSKNTSYYSFQENGSL"
     gene            2631..3173
                     /locus_tag="IV250_002647"
     CDS             2631..3173
                     /locus_tag="IV250_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769881.1"
                     /translation="MGERTQALITVKYGNSKTKASVSIHYQWGYGRYMLMDVLNQGIY
                     LINKAYDLRDKSREELAKGIFDRSTGVINFATYESGMSAYDETGFDGRYEPYQETIED
                     VMKFSDNNDGYAHLEMVFDENGYSLDDKTRLRLFDIEGKQIGLNKFCKNGKEFATKDF
                     IKGYRLLLENYGINLEESVK"
     gene            3174..3740
                     /locus_tag="IV250_002648"
     CDS             3174..3740
                     /locus_tag="IV250_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769882.1"
                     /translation="MFQKRKDKFPNLEVKSQKVIDGEMIELFQKNYGTIETTASCKYY
                     FQGKRNTFKGFADSDEELAKKIIDWLVSSEHVAREDTVRTTINGEVLTGTRCVCCNHE
                     FVEDEGCYRINEYGDFICQSCHEIEFPTERSWFEYMLQDYEMEKENGEVVFCTKEELS
                     KRSDDEIEDWCVWLVGADDCVAYYTTAE"
     gene            3755..4003
                     /locus_tag="IV250_002649"
     CDS             3755..4003
                     /locus_tag="IV250_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769883.1"
                     /translation="MEGMDFLDHEDLVDFGYTWKGMVGISRSLANAFYERNYAVYVLY
                     DDDTENLVDEEYKLDLENVLYGIEKEDLAKYIFSWLGQ"
     gene            4023..4262
                     /locus_tag="IV250_002650"
     CDS             4023..4262
                     /locus_tag="IV250_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769884.1"
                     /translation="MTKFKSKGMQWCSYYYVGNDEDLLRKTQRKGYPDKILKQIGYYM
                     PEERIEKEFCEVLFDSEQLLVLLTQNKLICQFENN"
     gene            4633..4914
                     /locus_tag="IV250_002651"
     CDS             4633..4914
                     /locus_tag="IV250_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HAP8769885.1"
                     /translation="MKVKNPTEKSRIQVGIDKNLKENAEMILEELGLNPTTAITILYK
                     QVVARGEFPVEIKLSEEEKQGIRLQQLTKDMPVDVLDTDEKLEEWFDEA"
     gene            4904..5245
                     /locus_tag="IV250_002652"
     CDS             4904..5245
                     /locus_tag="IV250_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP8769886.1"
                     /translation="MKPNEIVTVYVAFTDKNGGKRRPILVVSDKEDRVEFFGITSQYE
                     KKSDQIKRVYFPIGEWEKAGLKKQSWIDVGSLKAIPKSRENISFKKVGMLSASDTKKL
                     NKFIERLQRKV"
BASE COUNT         2170 a          767 c         1162 g         1652 t
ORIGIN      
        1 aaaaagatta ttggggtgag tcaaaagggt ttatttatcc agattcaaac atgggaggga
       61 actggaaagc tacaaatccc aaaaaagagc aagaagccat gaaattaaag aatacaaaaa
      121 gtaataattt attatatgct acgtgtaaac attttcgaca tgtacgagat acagaattta
      181 cgagttatca tctttcagga atagtaattg acagtttcgt atatgaggct atgggaaatt
      241 ggaaatttgt tgaaaataac agtgggggtc aaaacattag ttctgtatct tatgagacag
      301 ctttattaga atactataac tcacataaag ttatgggcgg tttaaattta tactctcctg
      361 gaagcaatca atttgttaat tctgatagta gtatcatatg tttagaaaaa gtattaaaaa
      421 aaatagcttt ataaagtttt atggaggaac aacatgggag acgaaaaaaa gcgcttggaa
      481 gttcaattac gtgaagcata tggacgagta acgtatactt acaccactca tttaaagcaa
      541 gccgataata ttatttctga caataaaatt ttaaggtgtc ttcaaattgg tttgtcagcc
      601 cttactactg gagggatact tagtaccatt attactgata atagtgagtt aacctttgtt
      661 gctggattat gttcaacaat ttctcttgga ttaaacttat actttaagaa ctttgatctt
      721 gctgaagaag ctaaaaatca tcaaattgtt gccgatgaat tatggttgat cagagaagaa
      781 tacgtaagct tactaacaga tttaaatcgt ttaagtgttg aagatattgt gaaaaaaaga
      841 gatcaattaa tgaacgaaac tcatgaagca tataaaaatg cccctaaaac aaatagtaaa
      901 agctatgaat cagctcagaa agcattaaaa tctgaagaag agcaattttt tacagaagag
      961 gaattggata agatgttacc agagcatctt agaaaaaaga gttctaaata ttgaactctt
     1021 tttttatgct taaaattctc ttgtaatttt tctattcaat caataaataa tctatattta
     1081 agaagaaata tgatgataga aacatctcct aaagccaccg caagccgttc taagcatagc
     1141 ttagcccgac attgctagat atgctcatct caccatgaac gtttcctaaa gccaccgcaa
     1201 gccgtcctag tcattgccta gtccgacatc gctagattag tgcatctcgc cataaaagtt
     1261 ttctaaagcc accgcaaacc gtcctagcca tcgcctagtc cgattttgct tgatcggttc
     1321 catttctgtt tatttagtgg gtcaacaccg taatcttaaa tagccatcgc ctatttaaga
     1381 ttactaggtc tgctttgaag gtaaaaagtc tttcattctt caagactttt aggctttcaa
     1441 gctacgcctg aaagcattga aattcatcgc tctttatttg gttttgccat ttataattcc
     1501 ttacgcccat tatctttttc gcctgtcttg ccttgtcaga agtgtttaga gcttatctca
     1561 tctttggatt aatacaagtg gcgtaaattt ttttctaaac gcaagcgtta cgaaaataat
     1621 ttacttaaac acttgtatta atccggcaga taagaacgca ttctaatcac tcccgacaag
     1681 gcaagacagg cgaaaaagat aaataaatga acgaaaggaa gaaacataaa atgcaaaacc
     1741 aaatagctat gaagaaaaaa acacctccct ctaaaacaat ccaaacaaaa aagaattttg
     1801 aaaaaccaaa aatcaaaaac agaaagaagc gttcaacatg agagatagaa ttttagaagt
     1861 taatcaagaa atggtaaaca agttaacaaa tatcagtggg aactatgaaa agtacgatgg
     1921 tactttactt gataactata tttttcacga tacacaaaat atcagaattg gcagaagtaa
     1981 accgagagcg ttcattctca tcagagaaaa atatttgaat gaatggaata gcacctatga
     2041 aatggttatg actgataact ttgaaaaggt attggatttc agaaatgctt tgttgggttc
     2101 tgatatggga aatagcagtc ttgaatctat tgaagttgaa aacaagaccg agaatgaagg
     2161 tgaaggattg attaagaatg tcttgaaaat cgaacaaaaa tatatcagtg aagatagaag
     2221 tttttataga gtcacaagct cggttggtct agcaaacttt gctactaact taattggaaa
     2281 caatgctagt gaatctttgc tagtgatgtg tctgaataca aagaatgagg tcattgctta
     2341 tagcgaagtt ttcaaaggtg gaattaatca atcagtagcg ataccaagag agatttttca
     2401 actagctttg ctaaacaatt cagcaaaaat cgcagtatca cataatcatc cgagcggata
     2461 tgaatttcca agtgaaaatg acaaaagttt tactacgaat ttgaaaaacg caggaaaaac
     2521 aattggtatt gaattgttag accatatcat cgtaacaagt aaaaacacaa gctattactc
     2581 atttcaagaa aatggttctt tataaaaatt agagaaaaga ggaaaacaag atgggcgaaa
     2641 gaacacaagc gttaattaca gtcaaatatg gtaacagcaa aacgaaagca agcgtgtcga
     2701 ttcattatca gtgggggtat ggtcgatata tgctgatgga cgtattgaat caaggaattt
     2761 atttaatcaa taaagcatac gacttacgag ataaatcaag agaagaatta gcaaaaggaa
     2821 tctttgatag aagtacaggg gttatcaatt ttgccaccta tgaatcaggt atgagtgctt
     2881 atgatgaaac aggatttgat gggcgctatg aaccttatca agaaacgatt gaagatgtga
     2941 tgaagttttc tgataacaat gatggctatg ctcatttaga gatggtgttc gatgaaaatg
     3001 gatatagtct agatgataaa accagattgc gattgtttga tattgagggt aaacagatag
     3061 gactaaacaa gttttgtaag aatggaaaag agtttgctac aaaagatttt attaagggat
     3121 acagattgtt gctagaaaat tatggaataa acctagaaga gagcgtgaaa taaatgtttc
     3181 aaaaaagaaa agacaaattc cctaatttag aagtgaaaag tcaaaaagtg attgatggtg
     3241 aaatgattga actattccaa aaaaattatg gaacgataga aactaccgca agttgcaaat
     3301 actactttca aggaaagaga aacacgttta aaggctttgc cgattctgat gaagaattgg
     3361 caaagaaaat cattgattgg cttgtcagca gtgaacacgt agcaagagaa gataccgtta
     3421 gaacaacgat taatggggaa gtcctaacag gtactagatg tgtttgttgt aatcatgaat
     3481 ttgtagaaga tgaaggttgc taccgaatca atgaatatgg tgattttatt tgccagagtt
     3541 gccacgagat agaatttcca accgaaagaa gttggtttga atacatgcta caagattatg
     3601 agatggaaaa agaaaatggt gaagtagttt tttgtaccaa agaggaattg agtaagagaa
     3661 gcgatgatga aattgaagat tggtgtgtgt ggttagtagg ggcagatgat tgcgttgcat
     3721 attacacaac agcagaatag gagtgtgaat cagcatggaa ggaatggatt ttctagatca
     3781 tgaagatttg gttgattttg gttatacatg gaaaggaatg gtaggtatta gcagaagtct
     3841 tgctaatgcc ttttacgaaa gaaattatgc agtatatgtt ctttatgatg acgatacaga
     3901 aaaccttgtt gacgaggaat ataaattgga tttagagaat gttttatatg gaatagaaaa
     3961 ggaagattta gcaaaatata ttttttcatg gttgggtcaa taagtgaaag gaagatagaa
     4021 acatgacaaa atttaaaagt aagggtatgc aatggtgttc ttattattat gttggaaatg
     4081 atgaagattt attgagaaaa acccaacgaa aaggttatcc agataagata ttgaaacaaa
     4141 taggttatta catgccagaa gagaggatag aaaaagaatt ttgtgaggtt ctttttgata
     4201 gtgaacagct attagttttg ttaacccaaa ataaattgat ttgccaattt gaaaataatt
     4261 aagatgaggt ggtgaaccat gaatgaagat aagatggtat taagtgaata tgctagattg
     4321 agaactatta attctttagc atctcattta agttgttctt tccttacaaa ggtgctaaaa
     4381 ttaatttaca aatgtgaaga ttttgtacaa gagaagcaca aagacaagcc tactgcgaat
     4441 aggcttgtct ttttttcatt cattacgatt ccgtatgtgt gggttagcga caatttgttc
     4501 aattaccgta tgatatagca tcttaatttg attagattac ttgcaatgta gtttattaag
     4561 agtataatat aatcataaat gtggataatt tgagaaataa tgagtgtttt atactcaaaa
     4621 ggagtggatg aaatgaaggt caaaaatcca actgaaaaaa gcagaatcca agtcggaata
     4681 gacaagaatt taaaagagaa cgcagaaatg atattggaag agctaggttt aaatcctaca
     4741 acagcaataa caattcttta caaacaagta gtcgctagag gtgagtttcc agtagagata
     4801 aagctaagtg aagaggagaa gcaaggaatc agattgcaac agctaactaa ggacatgcca
     4861 gtagatgttc tagatacaga tgaaaagtta gaagagtggt tcgatgaagc ctaatgagat
     4921 agtaacggtc tatgttgcat ttacagataa aaatggtggt aaaagaagac ctattttggt
     4981 ggttagtgat aaagaagata gagtagaatt ttttggaatc acaagtcaat atgagaagaa
     5041 atcagaccaa ataaagagag tgtattttcc tattggagaa tgggaaaaag caggtcttaa
     5101 aaagcaatca tggattgacg tagggtcttt aaaggctatt cccaaaagcc gagagaacat
     5161 atcatttaaa aaggttggta tgctatcagc tagtgatacg aaaaagctaa ataagtttat
     5221 cgagagatta caaagaaaag tctaacaagg cttttctttt tttgcttata tgtatattaa
     5281 gatatacgtc tatacaaacg tatttatgta tataaaaata cacgtaaata tatacgtgaa
     5341 tataaacatt aagaaaatat taagaatgat taaatagtgg taattgttaa tatacgaatt
     5401 ataagatata ttttaaatcg aatgtatgta aataaataaa atgactggag gatatttaat
     5461 agtgataaat aacagtaatc cagacggata cagtgagtga agtagcttgc tacgaacgaa
     5521 caagcagaat gaaacggtag tttcattaga gcaagtcgct tgcgacgtag cgttaccgct
     5581 gatgtaactg gcaagttaca tagagagagt ttgcttgcaa acgaacgtta cctacttgaa
     5641 tagaaaacaa aaataacaga ttagtgaatt ttaaatagga gccacaactc aaccaaccgc
     5701 ttgcggtggg tgagtcccac ggaggaaaag acgaacgaac ggtcgcgtag c
//
LOCUS       DACDIE010000109         5476 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.112, whole genome shotgun sequence.
ACCESSION   DACDIE010000109 DACDIE010000000
VERSION     DACDIE010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5476
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.112"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(43..409)
                     /gene="ssrA"
                     /locus_tag="IV250_002653"
     tmRNA           complement(43..409)
                     /gene="ssrA"
                     /locus_tag="IV250_002653"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(580..1353)
                     /locus_tag="IV250_002654"
     CDS             complement(580..1353)
                     /locus_tag="IV250_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8769887.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            1497..1847
                     /locus_tag="IV250_002655"
     CDS             1497..1847
                     /locus_tag="IV250_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAP8769888.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            1828..2544
                     /locus_tag="IV250_002656"
     CDS             1828..2544
                     /locus_tag="IV250_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8769889.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            2544..3992
                     /locus_tag="IV250_002657"
     CDS             2544..3992
                     /locus_tag="IV250_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8769890.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4062..4571)
                     /locus_tag="IV250_002658"
     CDS             complement(4062..4571)
                     /locus_tag="IV250_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769891.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(4561..5286)
                     /locus_tag="IV250_002659"
     CDS             complement(4561..5286)
                     /locus_tag="IV250_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769892.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
BASE COUNT         1863 a          955 c         1095 g         1563 t
ORIGIN      
        1 aaataaggat ttaagcatct agcgaaatct gctgaaatct cacaatggag acggcgggag
       61 tcgaacccgc gtccaaacac atcgccactt aaaaatctac gttcatagtc aactcattta
      121 aggattcact ttacagcttg ccgagtgaca ggcattctgt atcgctagtc tgatgttctc
      181 tttttcaact tacagacgga aagttgaaac gtatcccact aaagttagga cccttactcg
      241 agcacatggg cgatgccgag aggatctacg ctaactgttt ttaggcagct aaagcgtaag
      301 agttgttttc gtttttagca gttatattta actgtaacgt ttttacgtag acgtaaccta
      361 cgaaacgcaa ttcaagctcg actgtgcctg tcgaatccgt aacgtccccg ttagctaaca
      421 agggttacgt gtatttttca tacaaaatta gtatagcata tattgggata aattcaataa
      481 aaaagcgaat catatcccat tatttatata ataactgccc ctttcttaag aaaaaataag
      541 cgattttctt aagaaaagag gtagcttcaa aaaaagatct taaatcagat caaaatactt
      601 caatgcgtgg acaattccgc cttctgtatt tttctttgtg ataaaggtag aaagttcttt
      661 taatccgtca taggcgttgc ccatagcgat tttgttgtcg caagcttcca acaaggcaaa
      721 atcattcgga ccatcgccaa atccaaaagt aggaactttt tccagctgaa gttctttttg
      781 cagacgtttg acacctgccc cttttgagat tcctttcttc actgtatcga tcgagtaagg
      841 accattccga taaaaagtta gctcaggaaa atgttcgtga tagtattcgt cacctgattg
      901 agataagatc agcaacatat ttactgtgtt ttctttaaag cctaaaggat caatttccgg
      961 aacatctgaa tgaatgaagt cgtaagcatt gatcattgtt tggctatgac cggtacacca
     1021 gattttctga tcgttatagt aagaaagttc atgaccgttc aactgtacat gatcatacat
     1081 tttttgacat tcttctgaag aaaatgtttg agaatagatt tcttcgccgt ctactcgaat
     1141 gaaagaacca ttcatggtga ccgcagaagt aatgcccgca tcttccatga tagagtggat
     1201 ttctgcttct gtacgtcctg tagcgatgac ggggagaaca ttgttttctt ttaatgcgtt
     1261 catagctttt gccacttcgg gtgtgatttt tgatttcgca tctagtaatg ttccatcaag
     1321 atcaaagaaa gtgattgcac gataatttga cacaaataaa actccttttt tattggaata
     1381 agtttatctt aacaaagaat gaacaaaaga caaacctttc gtgaagaatt cctgaacata
     1441 ttggttcagt caacaattta gagtatacta taagaagaga atttaggagg gaaagaatga
     1501 tttttttcag tattggtatc ttattcttaa taattggcag tctttttcgg attctgccct
     1561 caaaaggaaa tctgcctttt tatgggtatc attcgcctct tgccgcaaaa acagacgctc
     1621 attggcggct ggctcaaaag acaagcggaa actggttttt tcttatgggc ttactgatgg
     1681 cattgatcgg ctattattta aagacatctg ggcatacgaa ttattttctg attgaaatgc
     1741 ttctgctagt atttccaatt atgcctattt ttattatgac agaaaagaaa ttacagaaat
     1801 atgacctgga aactggaggg aacgataatg aatattttaa tgattgaaga taatcaatct
     1861 gtttctgaaa tgatgcagat gtttttctta aatgaaggtt gggaagcaac tttcaagtat
     1921 gatggaaaag aagggctcga tgccttttta gaaaacccac aacattggga tatgattaca
     1981 ctagacctga atttgcccac actggacggt atgctagtag gaagagagat ccgcaaagtt
     2041 tcaagtactg tgccgattat catgctgaca gctagagatt ctgaaagcga tcaggtgatc
     2101 ggcttagaga tgggggcaga tgattatgtc acaaaacctt tcagtcctct gacgcttatt
     2161 gctcgaatga aagctttgca tcgccgtagt gagttggcag aagcaagaga agaagcagta
     2221 gtttcagatg atcattttga tatcgagact agtcatttca agatgaatac gaaaacacga
     2281 gaagcgtatc tggacaaccg gccaattgaa ggactgacgc ctaaagagtt cgatctattg
     2341 tttactttag caaaaaaacc acgtcaagta ttctctcggg agcagttgct tgaattagtc
     2401 tgggattatc agtactttgg tgatgaacgg acagtggacg cccatatcaa gaaattacga
     2461 caaaaaatcg aaaaggtcgg accacaagtc atccaaactg tttggggagt cggctacaaa
     2521 ttcgatgatt ctggtgttgc ctaatgcgtt atttgtatca gcaactctta gctttctgcg
     2581 gaatggtcgc gttgatctta ttgatcgttg gagtttcttt tacacaactg acaaaacaaa
     2641 cgattgaaga aaacaattac cagcaactgt ttggatatgc tgaatcagtt gaaaagacaa
     2701 cgcaaaccta tgccgatacg ctcccgcagt ttagtcaaga tcaggcgttt caaaatgcat
     2761 tgctgctgac agagcaagta ctgacagaac aaaatgtgaa ctttatattt atcgataaaa
     2821 acgaacgtgt catttatccg actaatacag ctttactgaa ttttacctta acagctgatc
     2881 aatggaaaaa tctgaggaat ggacgtcaag aaaaattcac atcgaataaa aatattttag
     2941 gacaagcaca ggcgacctct tacgcacttg tgccatttaa tttgaatcat gaattctatg
     3001 gagcacttgt agttagtcaa cctgcgcgaa atatcgataa cagcgttcgt ccaattacac
     3061 tgaacctgtt caaaggattt attttttcca gtatcgttgc agttattgcc agctatgctt
     3121 ttgcagcatt ccaagttaag cgtatcaatc ggttaaggaa tgcgacgaaa gaagtgacga
     3181 atggtaattt tgatgtacag ctccccgtac atgacaaaga tgaatttgat gagttagcag
     3241 atgatttcaa taaaatgacg aattcattga aagaatctca agcagaaatt gaagaacaag
     3301 aaaatcgtcg aagacaattt atggcagatg cttctcacga gatgagaaca cctcttacga
     3361 caatcaacgg acttctggaa gggttagaat acaatgcaat ccctgaaaat cagcgagaaa
     3421 acgcgattaa attgatgaaa aatgaaacag agcgtctgat ccgtttggtg aatgaaaact
     3481 tggattatga aaaaatccgt acgaatcaaa tcagtatggt cattaaaaaa ttcaatggaa
     3541 cagaaacatt gaaaaatatt gttgcacagt tagaagctaa agcagaagca gcaggagata
     3601 cattgacatt gaaagccgtt gatgatattg atgtttacgc tgattatgac cgatttgtcc
     3661 aaatcatggt caatattatt caaaacgcga tacagtttac tgaaaacggc caaatcacga
     3721 ttactttaga aaaaggctat cttgaaacga tcatcacgat tgaagatacg ggaattggaa
     3781 tgtctgaaca acaaatgaaa agtatctggg atcgttatta taaagtagat ccttcaagaa
     3841 aaaatacaaa atatggtgaa tcaggattag gtttaccaat cgtgcagcaa cttgtacgct
     3901 tgcataaagg aaaattagaa gtagaaagcg aactaggaaa aggaacaaaa ttcgctgttc
     3961 gtttgcctga cgtagaaatc aaaaaagagt aaaaaacaag cggtataaaa gatcatgaat
     4021 cgatcttcta taccgcttgt ttttactata aaatgattga tttagcccgc gggctctaaa
     4081 cgatcaatat aaggactttg agcaatagta gcatcatgag taaagagtaa gataccttga
     4141 ttttcttcat gagctaaata agaaagcaag tggagtagct cttggatctc caatacacta
     4201 agttcatcaa atgtatcttc aataacaata ttgtttcgct tggaaagcaa ggcatgaagt
     4261 aattgcagct ttactttttc aaaagacgtc aactcagctg ccgcacaatt aagcaaggaa
     4321 ggagagatat caagtttttc ttgataggcc aaacgatctt gtttgatttc tttttccggt
     4381 acatccaaca ggacatggtc tttaagattc agataaggaa acatcggcca atttttttgg
     4441 atgatcgcaa aattatccag ttttgcaaca agaacctctt ttttcaagtg ctccaatatt
     4501 ttatgctgtt tttcgagagg acacaaaata acggtcaagt gttcagcatg aatcaaatat
     4561 tcaggtttca ttacgtccag gcctcctact ttgcaaatga tggataaaaa aagtgaaagg
     4621 gaatagacta agaatgagac agcatccact gagcccaata agagtttggg caaatccctt
     4681 aagtatatct gtgaaaaaaa gatcattttg gaaatcgatg gtaaaaaaag tctgttgatc
     4741 catcggaatt gtaagtccgc ttaccgaagt caagatatca cggatatctg gcagttcagt
     4801 agtctttaag acaagaaaat tgatcctttg aagaagcgct tcataaactg actggaagct
     4861 tacgatccac aataaaacca agatacaaca aagaaagaga ggaaagaaca gctgccagaa
     4921 aataaaaagg aataatttgg ttttagaaag accagtcagg tgccaattga tcaattcctg
     4981 cttgttttgc ctgactgaat agtaaaagaa aaagataaaa aggaaggcaa atagaaaaaa
     5041 taaactgaat atgcctttta tataaaatcg gctagctaat tctaaagccg gaagaagctc
     5101 tgtagatgca gaagcgccaa taaatgaaag ccgatcttgg acttgattgg ctaaagccga
     5161 ttgcaaatgg taaagagtca ggcatgctat caataaaaaa gcagaaaagc ttgaaaataa
     5221 acaatagagt attgtctgct ttttgtaata aattaatcga gcagtagcat agcggattat
     5281 tttcatactt ttcacctcta taaaaagtat agaaaatgaa tatgaataaa gtatgaaatt
     5341 cctttagtga aatcacgaat taaaaccaga aaatattttg taaaaagcaa gaggttgtga
     5401 agaagctgcc ctattctatt accgtagatg caagcttttg aacactgttt attcgaaaag
     5461 aggagcagcg acaata
//
LOCUS       DACDIE010000110         5357 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.113, whole genome shotgun sequence.
ACCESSION   DACDIE010000110 DACDIE010000000
VERSION     DACDIE010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5357
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.113"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            23..694
                     /locus_tag="IV250_002660"
     CDS             23..694
                     /locus_tag="IV250_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8769893.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            753..1193
                     /locus_tag="IV250_002661"
     CDS             753..1193
                     /locus_tag="IV250_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769894.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1205..1873
                     /locus_tag="IV250_002662"
     CDS             1205..1873
                     /locus_tag="IV250_002662"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP8769895.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1937..2560
                     /locus_tag="IV250_002663"
     CDS             1937..2560
                     /locus_tag="IV250_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAP8769896.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2616..3002
                     /locus_tag="IV250_002664"
     CDS             2616..3002
                     /locus_tag="IV250_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HAP8769897.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3215..4606
                     /locus_tag="IV250_002665"
     CDS             3215..4606
                     /locus_tag="IV250_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAP8769898.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT         1776 a          883 c         1124 g         1574 t
ORIGIN      
        1 tgatgaaaaa agggggagaa aaatgaattt tttagttgtt ttaaaagata ctctgattga
       61 gcgatctaca tttctatatg ttcaattgca ggaaaataaa acattgctgc atttttcacc
      121 agagttccat ctagaggggc taacattagg agaaatctat caagcagaag ggctgtcggc
      181 tgttttgcac ttttttaagg tagaattgga attaccagta aaagattata tcgagctttc
      241 tcttgaatct tgggatcgag cagtggttga actgtttcca gaaggtttaa tgatcgatac
      301 aaacgatggg aagatccatc tgactgcagc agaactgcaa aaacaaatgc gttatcaaat
      361 agatgacgaa aattcttttt ctatttttag acgtcagcaa aagattctaa aaagcttttt
      421 aaaagtgatc agtaaaaaac ggaatctgat caaaacgaca cagctgttga aaaaatatcc
      481 aggtctgctt cacacgtcta ttcaatttcc tcaattgatt acgatgatcc atcgttttat
      541 acgtatgcaa cagctaccat ctaagaagct gtttcttcca ttgacggaca catttcgagt
      601 agtcaacaga gaagataaaa agaaagtgat tgtgattgac tttaccagaa atcggaatgt
      661 ccttcaccaa gttgcaatgg aaaaggccaa ttaaggcttt ttctttttgg atttttttgt
      721 cataataaat attagatgat ggaggagata atatgatgca aacatggtta gaaaggctaa
      781 tacgatttca gcagaactat cacaaactta tgcagggaag atatgcgaaa tttgatcagt
      841 tgaatcgcgg tctattgatc gctgcgcttg tctttagttt gctaagtcgt tggttgccct
      901 atagaatcgg acaattgttg ttcatcgtct ttttcggatg gatgatattt cgtttcctat
      961 caaaaaaaat ctaccccaga ctaaatgaga atcaacgtta tttgaaatat cttgatagag
     1021 tcaaagaaaa atatcatcag ataaaaaaca aaaagcaagt caaaatggat cgaaaaagtt
     1081 atactttttt tgagtgcccg aattgtcatc aaaagcagcg tgcacctaaa ggaaaaggac
     1141 gaatacgtgt gacatgcaaa acatgtggta taaaattcga aacgaatgtt taagggagaa
     1201 aagaatgaaa ttagtattat tacgtcatgg cgaaagcgaa gcaaattttg aaaattactg
     1261 gacaggatgg ctagatgtcg ccttaacaga aaaaggaaaa gaacaagcag cagttgccgg
     1321 tcaaaaaatg cttgaagccg gacttcaatt cgatgccgtt tatacctctg ttttgaaacg
     1381 tgcgattctt accggacaag tcgcactaga agaaatgggt cagttatggc ttcctataat
     1441 aaaaacatgg cgtctaaatg aacggcatta tggggatctt gtgggtaaaa acaaagataa
     1501 gatgaaagaa atatacggaa aagatcaagt aaaaacatgg cgcagaggat atacggaagt
     1561 tccgccgtta accgcagaaa atagatttga ccgcagatat gatcacttgg atccgcattt
     1621 gatcccatac ggagaaagtc tggaaatgac agtgaatcgg atcattccct tatggcaaga
     1681 tcatctagca ccaaaactaa aagatcggca agatttacta gttgtgggtc atggtaatag
     1741 catccgagca ctgacaaaat atttagaaga tgttcctgaa gatcaaatgg atacgatcga
     1801 tatcccaaat gcacaaccaa tccagtatac attagcagat gatttgacaa ttctggacaa
     1861 aaaaatactt taaagaacac ctttatctaa aagtgagaga tagaaaaaag aagcaaagga
     1921 atggaacaaa aggaacatga aaaattatat atgggacttt gatggtacat tgtttgatac
     1981 gtatcccgca atggtagatg gtgcatggca agcactaaaa gattttggta tcagtatgga
     2041 caaaaaagaa atctacttca aaatgaaaaa gtattctact agttatttga tcaatgaatc
     2101 aaacttgaat gccagagagt ttaatgaatt atttcaccga tatgaaaaag aaagtacgga
     2161 agtttcccgt ccatttccag aaacgaaaca agtactggaa atgctaaaag acaacggcgg
     2221 tcgtcatttc atcctgaccc accgtttgac agaatcaaca tggggcctgt taaaagaaca
     2281 tcgattggca catctgattg aggaagtagt agggatcgat caggactttc ctcgtaaacc
     2341 tgatccagca tctttaaatt atttgatcga tacttttcat ttagaaagaa cagacacgat
     2401 gatgatcggc gatcgtcgtt tagatatcga agcagggaaa aatgcaggag tcgctacttg
     2461 tctttacgac atcgatcatt ttctgggtga gatcccggca gattatgtgg taggaaatct
     2521 gaatgaaatc cttaccttgc agagcatcat taaaaagtag tatccccttt caaaggagat
     2581 atgttataat tttcataaag gagtgataaa attatatgga aatcagaaga caaaaagaag
     2641 ttgtagaagc atttcattat gatatgagat tgccgaacca agaagtagaa acagatttga
     2701 aagtaggctt ctcgccttta aaagaaacga tcgaaaacta tccgaaagaa catagcatcg
     2761 taggagcacg cttagagttc cgtttagtgt ttgatacata tgttttatct ggaagtgtga
     2821 gtcagatcaa tcatatcctc aatcggaaaa ttgaaggtca aaatgatgtc acacaagaag
     2881 aagtggacga gctagtagca ccgttattcg atatcgtacg ccgaatgtct tatgaggtga
     2941 cagagatcgc cttagatcag cctggggtaa aattgaattt tcaatcagaa aaacaagaat
     3001 agacagtaaa aacatacctg cgatagatta aagctatcac aggtatgttt ttttattact
     3061 aattgtaagc aaaaagaatg tcatttcgat aaaaatcgtc taaaatataa aagtcactga
     3121 aaaaaatttt ttcaggagga tggttgaaga atcattctcg ggaaccttta cttccttcga
     3181 tggacttgtc attaataata tgaggaggag aaaaatgaca acactcatca cagttttagt
     3241 ggttctagtt tttgcggcat tattatatgt gttctatcgt atgcaaaagc gccatacaaa
     3301 attttcaaca cgcgtcttta cagcattagg agttggtatc gtctttggcg gtctgcttca
     3361 attgatcttt ggtgtagacg acaaagtcgt tactcaatca atggaatgga tcgggattgt
     3421 aggtcaagga tatgtatcat tcttgcaaat gctagttatg cctttagtgt ttatttcgat
     3481 cattggtgca ttcacaaaaa tgaaagtttc tgaaaagcta gggaaaatta gtgcgactgt
     3541 attgacttct ttacttggaa caacagctat tgcagcattg atcggaattg gttcaacaat
     3601 gctgttcgga ctccaaggag ctaaattcac acaaggagct gcagaaacag cacgaatctc
     3661 tgaactggca acacgtcagg aaacagttga aaacttatcg attccacagc agattttatc
     3721 ctttatccct aaaaacgttt ttgctgattt agcaggtagt cgtgcaacaa gtacgattgg
     3781 agtcgttatt ttcgcggcat ttgtcggaat cgcttatcta ggcgtgaaaa gaaaagcgcc
     3841 aaaagaagca gcattctttt cagatttagt cgacagtctg tataaaatca ttatgcggat
     3901 cgtgacgctt gttttgcgtt tgacacctta tggagtattt gctttgatga caaacgtttt
     3961 ggcaacaagt gattttgcag ctatcgtcaa tttaggtaaa tttgtcgttg cttcttacgt
     4021 agcattgctt gctgttttga ttgtccactc actgattttg attggtgcaa aagtcaatcc
     4081 aatcaactat ttcaaaaaag cctttccggt attgagcttt gcgtttactt cacgttcaag
     4141 tgctggagct ttgccattga atatcgagac tcagacaaaa gctttaggag tagatgatgc
     4201 aacagctaat ttctcagcaa gttttggttt gtctattgga caaaacggct gtgctggcgt
     4261 ttatccagct atgttggcaa cgatcgtcgc accagcagct ggagtgaatg tctttagttt
     4321 ggaatttatt ttgatgcttg tggcaattgt aacaatcagt tcttttggtg tagctggagt
     4381 cggtgggggc gcaacatttg cttcactgat cgttttaggg gcaatgaact tgccagtagc
     4441 aatcgttggg ttagttatct ctgttgaacc attaatcgat atggcacgta cagcagtaaa
     4501 cgtcagcggt agcatggtag ctggtatcgt gacaagtgca cgcatcaaag atttagatcg
     4561 cgatgtcatc aatgatgatt ctaaagtgat cgatgtgaat gcatagtcaa aaagaggttg
     4621 tgatagaagt ggtcagcttt aagaaataag aagaagtttc ctaaaaatag tttctcaaat
     4681 ttttgtgaaa tttcggctta tttccgagaa gctacttctg aaacaccgtg aataagaggt
     4741 tgtgaaaaag ctgccctgca tcaagtaata agaacagcca aacaaaaata gttcctcata
     4801 ttttacgttt ggttgagctt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
     4861 aggtcgtgag cctgccgttt cactctgagt cacaaagagc aatttcaaaa aatagcttct
     4921 catgtttgtt gaatattgtg tcttgtgacc gaggagttgt caggagaaca ccgtttacca
     4981 gaggttgtga cttgttttta gtcacgacct ctttttttat ttattccata taaatacaca
     5041 ttatttattc cgaaaagtga aagcgcaaat aacaaaaaca accaacttat ttattattaa
     5101 agaaaagcgt atttaagtag gacgattctg ttgagagctt tcgtagattt tcccattttc
     5161 ctatggtaaa ctgacaaagt tgagaatgtt agaacagatc gtttagtcta atggtgataa
     5221 gtcaagatga aatttaagat tttctttatt gagtaatatg ttttgaagcg cacgaaacta
     5281 gacccgatca aaatcaaatt tttttcgggt tgattcaagg atttgcgtca ataacggatt
     5341 gttatttctc tatcaca
//
LOCUS       DACDIE010000111         5146 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.114, whole genome shotgun sequence.
ACCESSION   DACDIE010000111 DACDIE010000000
VERSION     DACDIE010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5146
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.114"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..197)
                     /locus_tag="IV250_002666"
     CDS             complement(<1..197)
                     /locus_tag="IV250_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAP8769899.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITR"
     gene            421..1221
                     /locus_tag="IV250_002667"
     CDS             421..1221
                     /locus_tag="IV250_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8769900.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            1240..3108
                     /locus_tag="IV250_002668"
     CDS             1240..3108
                     /locus_tag="IV250_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8769901.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            3101..3592
                     /locus_tag="IV250_002669"
     CDS             3101..3592
                     /locus_tag="IV250_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAP8769902.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            3580..4134
                     /gene="srlA"
                     /locus_tag="IV250_002670"
     CDS             3580..4134
                     /gene="srlA"
                     /locus_tag="IV250_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAP8769903.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            4152..>5146
                     /locus_tag="IV250_002671"
     CDS             4152..>5146
                     /locus_tag="IV250_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAP8769904.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         1803 a          818 c         1061 g         1464 t
ORIGIN      
        1 tttctagtaa tcattgggaa aaatttcaag cagtagatcg cacaaatacc tacgacgata
       61 taaatgatct tcgcaaaaat agttgttgaa ccaccagcaa tcatagctac taaatcaagt
      121 tcgaacaagc ctaccaacag ccagttcaac cctcccacaa tcaataaagt taatgcaata
      181 gcatctaaag ttttcattag aattctcctt tcttaaatga taaaacaatt atatatcaaa
      241 aagagaaaca ttgcttatat aacgctcagg aattttagca atctatgcaa aaagatgaaa
      301 attttttttg ctcttccatt agtacacatt ttttagctga agctgtaaat ttcatgtctt
      361 aatttttttt agtgagcttt tatactggat ttgtaagaaa aaagaaggag aggatcacat
      421 atgtcggatt ggttggatat tgcaggaaaa gtagtaattg taactggagg ttcctcagga
      481 attggaagaa gtatcgtcga aaatttgttg aagcagaatg cacaagtagc gaattttgat
      541 gtaactgaat gccgtataca gcatgaaaac ctactatctt taaaagtcga tgtgtcttct
      601 aaaacggaca tagaagaagg gatatataaa gtaatgaaac atttcaagac gatcgatggt
      661 ctagtcaata atgcaggaat aaatattcct tctcttctaa tagatagaaa tcatccaaaa
      721 agcaagtatg aattatcaga acaagttttt gataagatga tcgctgtcaa tcaaaaaagt
      781 gtatatttga tgagccaagc agttggaaga atcttggtgc aaaagggatc aggagtgatc
      841 gtgaatcttt cttctgaatc aggattggaa ggatcagaag gacagagctg ttatgctgct
      901 acaaaagcag cgatgaacag ttttactcgt tcttgggcaa aagagttagg aaagaggaat
      961 gttcgtgtgg taggtgttgc accagggata ctggaagaaa ccggtttgag aacacaagaa
     1021 tatgaagaag ctctttctta tacgagaggt ataagcgtcg aacagctgag aaacggttac
     1081 agtcgaacaa gtacgatccc tctaggaagg agtggtaagc tgcaagaagt ggcggatcta
     1141 gtctgctatt atctttcaga aagatcaagt tatatcactg gagtcacgac taatatttca
     1201 ggaggtaaaa caagaggata aaggataggg gggacgcacg tgacgctagt aaacagatgg
     1261 taccaattat tgaaattact agtcactcac aagaaagttt caatccagga agtgcaaaaa
     1321 gaaatcaaaa ccagtaatca aaccataaaa aaagatattg gggagttgaa tgaagaaatt
     1381 gccggtattg caaaaatcat ccaaaaaaac aaacattttg aacttgaaat ttatgatttt
     1441 gatttttttg atgagatcat gcaaggaaaa ttgaaaacag ccagtgattt caattcggca
     1501 tccaaaagaa tcgcttattt actaaatagg ctgatcgaaa cagaggattt cttacgtatg
     1561 gatgatttgt ctgaagaagt cggtgtcagc agaggaacag tggtcaagga tctaaagaca
     1621 ttaaaagaga tgatcaatga ttttgatgta aagattacag gcacaccaaa taaaggacta
     1681 agaataatag gagatgagct agaattacga ctacttttat tttattttgt ttctccctac
     1741 ttttcagaga cgaaaacaga acagtttcta gaagaaaaac aagcaatcaa acattttcag
     1801 aagaagacga atgctagtaa acaagaaatc acccttttaa cgaaagttat cggtatttct
     1861 ttgaatagaa tctctagtaa ctatctgttg aagaagccca tcaatcgtta cagtgggtgt
     1921 tttgaatata tccaagaatt caatgaactg atcaatcagt tggaagaaat ctatgaacta
     1981 actttgagcc aatatgaaaa agattttatc agttttcctt tgaacatcat caacacaagt
     2041 ataacgatga taaggaaagc taactctgat ttgcgcttat atttcgaccc catgatgatg
     2101 cgtattcgac gattagatct cattgatctg gatgagtcct tcttatatca agagatgaaa
     2161 cagcacctct ctttattgat aaatcgggta atttttcgtt atcgattgac tgatttattt
     2221 tatggtgaga tagaaaaaaa gtatcctttt tcttaccaaa tcgctagcga atgcatacaa
     2281 gcattagaag ctatcttgca acataagatc tctaaagtag agatcagcta cctaacgttg
     2341 tatttggagt tagcatttcg atatcaaaaa gagcattttt cctctaaaaa agtagccatt
     2401 gttcatacga caggaaaagg aacagcatta atgatccaaa gacaaatcaa aaggatattg
     2461 gggagggata tacaaataac acatgtttca gaagaacaat atgaaaaagt agatctaaac
     2521 aaatatttcg ctgtttttac aacgattccg ttaaaaaatg taaatgaaga gacaccggtg
     2581 atccatttga ctaatctttt tgatgactct tggttaaggg cacaatggca aaaagcaaaa
     2641 aaaaagcgat tagccgggtt tcattctact gtttttttct atcaaatatg gaaagaaaaa
     2701 aagacgtatc aagaatattt aaacaaatta gtcgctcctt tgatcagagg ctattatgtg
     2761 gatgaagaat ttgaaaaaag gatcttgaaa agagaatatc agcaaagcac atttgttaac
     2821 aaagaatttg cttttcctca cacaaccaac cagctatctc aacacattgt tttatcagta
     2881 gggctaataa ctgatcaagg tttagcagaa acaggaggaa atggagttat ttttttattt
     2941 gccatacctg aagaaatgac ggctgaagtt gagaatgaat tatttgagct atacgatatc
     3001 ctatttgaac tcataaaaat gggaatagat gagtttattg aattagcacc gacacaagaa
     3061 tcatttttaa aactaatcaa aagtaaagga gagagacttc atgaatgata tgtatctatt
     3121 catagttttc gcagcttcag cttatgtgct gcaagcagtt atcagttgga agcaactaaa
     3181 gcatttcaat caaacatata gcgaactgcg aagaaaagga aaagtagcga ttggtaaacg
     3241 acctgggaaa atccgttcag gcacgattgt tatgctagct atagacgatc atgatcaagt
     3301 tttagaagtc agaaaaatgc aaggggttac atctttggca aaatttcatt cattacctgt
     3361 ctatgcgggt gaagatatcc attacatcga tcgttaccat ccctcagttc gagcggaaga
     3421 tcgtttgaca caagcagctt tagaaaatgc cagagaagta tacttacgaa tagaggcagg
     3481 aaatgaaaaa agtgaacaac cttcattgcc ttttattgct atgctcaata attttcggat
     3541 atggaaaagt acgatcaaag aaaaaaggag tgacaaacga tggatatcat aacaaaattt
     3601 gctgagggat ttatgaggct gttccaaaca ggtgcagaaa cattcataag ctggatgaca
     3661 ggaatcgtac cagttgtctt gatgctgatg gttgcaatga atacgctgat tgctttttta
     3721 ggtgaagacc gagtaacgaa agtagccaaa ttatcagcga aaaatccgct tactcgttac
     3781 cttgtgttac cgttcttatc tgcttttatg ttgggaaatc ctatgtcatt tacaatggct
     3841 cgtttccttc cagaatacta caaaccaagc tattatgctg ctcaagctca attttgtcat
     3901 acgagcaatg gtgtattccc ccatattaat cctggagaat tgtttgtatg gatggggatt
     3961 gctcaaggga tcgaaacctt agggctaaat tcgatggagc tggctatccg atacttgctt
     4021 gtgggactgc tgatgaactt tattggtggt tggataactg attttactac tggatttgta
     4081 tgcagacagc aaggaatcgt tcttagcaaa aaagtagagc tttcagttga ttagagaaag
     4141 gagagtttta aatgtatggt gatattaaga tcgtaagagg aaacggcggg tatggaggtc
     4201 ctttgattat taggccgaca aaagataaac acaagttcat ctatgtcaca ggtggaggtg
     4261 agaggccaat gatcgtagat aaaatagaag aactgacagg tatggagtct atcaatgggt
     4321 tcaaaacatc aatacctgat agtgaaatcg cgcttgcaat cgttgactgt ggagggactc
     4381 ttagatgtgg aatttatcct aaaaaaggga taccaaccat caatatcgtt tccacaggaa
     4441 aaagtggccc attagcacaa tatatcactg aagatattta tgtttcagca gtagggttag
     4501 aacaaataca gccactaatg gaaactgatg ataacaaaga aacagtaaat actgcatctc
     4561 cagataaaaa gtacaaatat gatacttcta aaaagataac cgagcaaaaa gcagagcagc
     4621 aaaactggat agcaaaaatt ggaatgggcg cgggaaaagt agttgctaca ttcaatcaat
     4681 cagcaagaga agcagtagat acgatgatcc ggacagtgat cccttttatg gctttcgttt
     4741 cccttttgat tggtgtgata caaggttctg ggatcggtaa tgtatttgct aacattatgg
     4801 cacctttagc aggaaatgca gttggattaa caatcattgg ctttatttgt tcactgccat
     4861 ttttatctcc tttgcttggt ccaggggcag ttattagcca agtgattgga acattgatcg
     4921 gtgtagagat tgggaaagga aatattcctc cccaattagc attaccggca ctatttgcta
     4981 tcaatactca aaatgcatgt gattttattc cagtcggtct aggtttggaa gaagcaaaag
     5041 ctgagacagt agaagtaggt gtaccatcag ttctttattc aagatttctt aacggcatcc
     5101 cacgagttat cattgcttgg gtagcaagtt ttggattata tagctg
//
LOCUS       DACDIE010000112         4921 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.115, whole genome shotgun sequence.
ACCESSION   DACDIE010000112 DACDIE010000000
VERSION     DACDIE010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4921
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.115"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            107..1248
                     /locus_tag="IV250_002672"
     CDS             join(107..335,335..1248)
                     /locus_tag="IV250_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP8769905.1"
                     /translation="MAKAKKKFDEDFKKMILDLNQSGQSVEELAAQYGIATQTIYRWK
                     KLHTKNVATGMTEAEILAMKKEMARMQEENTIPKKGFNHIRSKVKMSDVFEFIQSNVH
                     DHDVKQMCTVLEAPKSSYYDWKKKKPSKRQSENKRLNRLISGIYFENKGIYGAPKIHK
                     ILVGRGETLSLKKVQILMRKLGLRSVTLKKYKPGKQAKVKSEGRKNLLNQDFTTTSIN
                     QKWVTDITYIHTLKDGWTYLSTIQDLHTKKVIGWKFGKQMTKELVIETLDNALLNQTP
                     SENLIIHSDLGSQYTSEAYEAKLNELNIRHSFSRKGCPYDNAGIESFHASLKKEEVYP
                     SKAYENFEAAYLALFQYIEGFYNRKRIHSSINYLTPNQMEELALQA"
     gene            1483..1935
                     /locus_tag="IV250_002673"
     CDS             1483..1935
                     /locus_tag="IV250_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769906.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            1949..2638
                     /locus_tag="IV250_002674"
     CDS             1949..2638
                     /locus_tag="IV250_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769907.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            2763..>4921
                     /locus_tag="IV250_002675"
     CDS             2763..>4921
                     /locus_tag="IV250_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HAP8769908.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTN"
BASE COUNT         1668 a          765 c         1011 g         1477 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc gtagaatcta actactaaga aaaggacgtg tccacgatgg caaaagccaa
      121 gaaaaaattt gatgaagact ttaagaagat gatcttagat ttaaaccagt ctggccaatc
      181 ggtcgaagaa ctggcagcgc aatatggtat cgctacacaa acgatttatc gttggaagaa
      241 gcttcataca aagaatgtag caaccggcat gactgaggca gaaatcctag cgatgaaaaa
      301 ggaaatggcc cgcatgcagg aggaaaacac tatcctaaaa aaggctttaa ccatattcgc
      361 tcaaaagtaa aaatgagtga tgtcttcgaa tttattcaat caaatgttca tgatcatgac
      421 gtaaaacaaa tgtgtaccgt tttggaagca ccgaaatcca gctactatga ctggaagaaa
      481 aagaaacctt ccaagcgcca atcagaaaac aaacgcttaa accgcttgat ttcaggaatc
      541 tactttgaaa acaagggtat ctatggtgcg cctaaaattc ataagattct tgttggccgt
      601 ggcgagacct tgtccttaaa gaaagtacaa atactgatga gaaaattagg cttgcgttca
      661 gtcaccctca agaagtacaa gcctggcaaa caagcgaaag ttaaaagtga aggacgtaaa
      721 aacctgttaa accaagattt taccactacg tcgatcaatc aaaaatgggt gacagatatt
      781 acctatattc ataccctaaa agatggctgg acctacctct ccactattca agacctccac
      841 acaaagaaag tcatcggttg gaagtttggc aagcagatga cgaaagaatt ggttatcgaa
      901 acccttgaca atgcgctctt aaaccaaaca ccctccgaga acttgatcat ccactcagat
      961 ttgggttctc agtacactag cgaagcctat gaggcaaagc tgaatgaatt gaacattcgg
     1021 cactccttca gtcgtaaggg ctgtccctac gataatgccg gtatcgaatc gtttcacgcc
     1081 tcactcaaaa aagaagaagt ctatccatca aaagcgtatg aaaactttga agctgcttat
     1141 ttagcgttat ttcaatacat tgaaggattt tacaatcgca agcgaatcca tagtagtatc
     1201 aactatttaa caccaaatca gatggaggag ttggcattgc aagcttagtt cctagctacc
     1261 acttacattt ttttggcgag atttccacag ggtttcaact tcatcgcttc gatcagcggt
     1321 caaacggttt tggtttgacc gctggtcgaa acgatgagat actcacccgc tggaatctcg
     1381 aaaaaattct cacaaatcgt gtccgaggta ttgactcaaa tccaccaatt atcttatcgt
     1441 agatatatat tataaattta ataagaaagc gaggtgagaa tcgtgaaaaa aacgatacta
     1501 agtatttttt cagtatttac aatattaatt gggggctatt ttcttttgaa tttagattct
     1561 gttaattcaa acgctcaaat aaaagatcaa agagtagctg aacataataa taaaaaaatg
     1621 agtttgacca aaaatatcta ttctatgtca gatgaagaat tgtataaaga atttggtgat
     1681 ttatccgggg aacagttaga agaaagtatt ccaggaatta gtgactcaga taaaattgtt
     1741 gtcttgtctg ctggtgggca attaatggga gatggcaaat ccgaattagt tagttataaa
     1801 caaaatcaca gacagtttat tgatgttaat actgattcca acgctatttc gattaaggat
     1861 ctaaaagagc ttttaaatag taaaagaaat gaattaaagc agataaaaga aactaattca
     1921 gtggaattag agtaaaggaa gattcattat gaaaaagatt tttggtgttt ctgttgggct
     1981 acttatgatg ttaacatttg gaaatgcagt atatgctgct gagaaagaag ttatttcaga
     2041 tccagagcag tacgcctcac gatactttgc tgattttgaa gaagaagata aagtacttgc
     2101 attgccagaa ggtggttttt taaaagggga agctaaaatt gtatctatgg ataatccaga
     2161 tataattcaa ggttcatata attcagaaac tgatcctgct tcagtttcag tgacagaagc
     2221 aaaaaaaatt gttgctgaaa aattcaaaaa tgatattgaa actactccaa ttatgcctcg
     2281 aggagcattt ccacccgatg ttggtactag cggacacctt cttgtactag caggaggagg
     2341 aagctataaa tcacagccat ttagtggtgc gggatggcgt tttggaggta tgggtgtaaa
     2401 accagctgat ggtacaggca actttttacg ttggaaatcc tacattgacg atggaagagt
     2461 aggaaatgta aatcaagcca acgcaactaa atctggttct attcaaggaa tggcattgta
     2521 tgctcgtgaa ggattcaaat actttgcacc aaacgggcct ggttggacaa tgaatatagg
     2581 tatttattac acttataatc ctgttgcagg tacgtattat gttgttcaaa atatttaata
     2641 aaacagcaca aagagatttc tttgtgctgt tttattattt ttagttgttg acaaacacta
     2701 aaatataaaa tataatcaac acataaacga acgtatgttc ggcaagaaag caggggattt
     2761 ttatggatga gatggtttta agtactcaga aatggctaaa caagaaatac agtaatgtta
     2821 cagggtttga taaggttcca gaaaatgggc gaacagggtg gcctacaatc tatggactta
     2881 ttgaaggact acaagtagaa cttggtatta ctaatctagt agctaatttt ggacctacca
     2941 ctgaaaaaat gtacgataat caggttactc ctaaatgggg caaaaatcta cctaaaaata
     3001 ttgtatttct tattcaaggt gccttttggt gtaaggggat taatccaggt ggcttcgatg
     3061 gggtatatac tccatattta gatactgctg taaaagagtt gcagactgat gcagggttta
     3121 atggaagcgc tgtaactgga gttttggatg cgaaatgggc caaagcgtta tttgatatga
     3181 gtgcttttgt tcttgttccc ggcggagata gtaaaattag aaaaatgcag caatggttaa
     3241 atgtaaatta tttagaatac acgggaataa tgccttgtga tggtatttat caaagaaatt
     3301 caaatcaagc attaattttt gcattacaag ctgaactagg atattctccc agtgaagcaa
     3361 cagggtctta tggtaatggg acaactagta aaacttatcc tgtgtctgaa ggtaactcgg
     3421 gaaactatgt aagaataatt caatatggat tatatgttaa tggttatttt gaagacggta
     3481 cattcgatgg tattttcact aaatatatgg gtttagaagt actggcattc cgaaaattta
     3541 tggtattgcc tgaatatact gaaatagcag atgttgttgt aataaaagga ctattgtcta
     3601 gcgctggaga tattagaaga tctgcagatg gcgcagatac ttctacgcaa ttaactagat
     3661 ctcaaatcca aacattggtt gataatgata taaaaatagt tggtagatat cttactggta
     3721 ctgttggaat tggaaaagat gaacgaaata agtatctaac tacagaggaa ttgaataata
     3781 ttttttctaa aaatttatct gtgtttccta tctatcaaga tggaggtgca gcattagctt
     3841 attttactta tgatcgtggc ttatcagatg gtaagaaggc tatagatgca gcaaaaaatc
     3901 taaatatacc attaacaaca gttatttatt ttgctgttga tctagacatg ttaggggaag
     3961 aaattcttgc atatgctgga gaatatttta agggggtatc tgctgtaatg tcatatagcg
     4021 ggtatcaaac gggtgtttac ggaacaagaa atgtttgttc tcaaataatc aataacgggt
     4081 atgcttcgtt tgcatttgtt tcagatatgt cgactggtta tagtgggaat ttaggttttc
     4141 ctatgcctag acaatggtct tttgatcaat ttgttgaatt taccatagga agtggcaacg
     4201 gagctgttgg tatcgattta atcgctacat cgggtagaga tagcggattt aatgagttat
     4261 caaatgatac taataatgat aattacattg ccaaatacaa tcagaaggtt ataaatcaga
     4321 tggtacgtgc gtatcaatat ctatcacaag ctggtcttga taatccttgg aaccctcatt
     4381 tgacatttta tagatatgta aattattctg gtttgtcatg ggacatcatt tcttctcctg
     4441 taacagagca tgatagaaaa atttatgatg aatataggaa taaattaact aatagcgagg
     4501 gcctgtataa ttattttata gatcctaata ctggttcaat aataggtttg cctcacttaa
     4561 ttgtgacgtt acaatcacag atgtttctag ccgacacaat caatgatagt gtttctgact
     4621 ttgcaggttg gttaggagat ttaatgactt gctggggtga agttaagaaa ttaggaatac
     4681 aaatggagca aggtgtattt gcattagtag gtacggctgc aggtggaaca tttagtctag
     4741 aagatttata ccaagatgtt gacgcggtaa atgtttatac cttagcaaaa agaaattctg
     4801 attcaacagc tttatcggct ttaaaaacgt actattgtga taagggtgct attgatagat
     4861 ttaatcaatt tgttaagaac agatttggtt cttcagataa aatcgcttca gtaacaaata
     4921 g
//
LOCUS       DACDIE010000113         4783 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.116, whole genome shotgun sequence.
ACCESSION   DACDIE010000113 DACDIE010000000
VERSION     DACDIE010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4783
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.116"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..740)
                     /locus_tag="IV250_002676"
     CDS             complement(<1..740)
                     /locus_tag="IV250_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769909.1"
                     /translation="MNKRKWLFVCVAALFSTVAYSQLSFADSSDSSTTQTIDNSITAP
                     SETDSSENTTEETADSNISFTESTNPTSESSSETSEKPQDVEDTQVIKQAIHIQKIIS
                     SNQINNQGSQVAQQEGINGAKFIVYDITNILEEMSQDDNEKNQSVEIIESKLKDRAKK
                     LSYDQLKKVAEGETKTIDDQEGVIEFSIEVPANKKQAYYIVNESSPENISNSEDIVLL
                     TPVSNEKGEFLTDVWLYPKSEKSEPKEE"
     gene            complement(737..1492)
                     /locus_tag="IV250_002677"
     CDS             complement(737..1492)
                     /locus_tag="IV250_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8769910.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPVVETNVAKFQQQQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSISLKLPIYDGTSDKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGQKFFIKVNEEQHAYQIYRIEEVK
                     AEELQKNYLDYLMPEKDQDRITLMTCTPKGVNSHRFLVYGERVSFSKEDLKLQKKAKN
                     RKSISLDWRLALLFAFFCVPIIFIYKKIRRRSE"
     gene            complement(1505..3481)
                     /locus_tag="IV250_002678"
     CDS             complement(1505..3481)
                     /locus_tag="IV250_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP8769911.1"
                     /translation="MAINRYVKKAGMLVGMLGIIAGCFQGYQVHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGADQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGGLSAVVYFKKKA"
     gene            complement(3534..4205)
                     /locus_tag="IV250_002679"
     CDS             complement(3534..4205)
                     /locus_tag="IV250_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP8769912.1"
                     /translation="MKKWLFGFLGVTLIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPGYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            complement(4265..4474)
                     /locus_tag="IV250_002680"
     CDS             complement(4265..4474)
                     /locus_tag="IV250_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769913.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVAQYINDMQLT
                     GKEKACLLGSMKNFEQLRRTYRIHF"
     gene            complement(4541..4780)
                     /locus_tag="IV250_002681"
     CDS             complement(4541..4780)
                     /locus_tag="IV250_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769914.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKEVFKALSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
BASE COUNT         1269 a         1008 c          727 g         1779 t
ORIGIN      
        1 atttcttctt tcggctctga tttctctgat ttagggtata accaaacatc cgtcaaaaat
       61 tcaccttttt catttgaaac aggtgtcaat aaaacaatat cttcactgtt tgaaatgttt
      121 tcagggcttg attcattaac gatatagtat gcttgttttt tgtttgcagg tacttcaatg
      181 gaaaattcaa tcacgccttc ctgatcatca atcgttttgg tttctccttc agccactttt
      241 ttcaactggt cgtatgaaag tttttttgct cgatctttca attttgactc aattatttct
      301 acagattgat ttttctcatt atcatcttgt gacatttctt ctaagatatt cgtgatatcg
      361 tagacaataa acttcgcgcc attgatacct tcttgctggg ctacttgact gccttgattg
      421 ttgatttgat tcgaagaaat aattttttga atatgaatgg cttgcttgat cacttgcgta
      481 tcttctacgt cttgcggctt ttctgaagtc tctgaagaac tttctgacgt tggattcgtt
      541 gattcagtaa agcttatatt tgaatctgcc gtttcttctg tagtattctc cgaactgtcc
      601 gtttcgcttg gtgcagtaat tgaattatct attgtttgag tggtactact atcactgctg
      661 tcagcgaaag acagttgcga ataagctaca gtagaaaaga gagctgctac acaaacaaac
      721 agccattttc tcttattcat tccgatctcc tccttatttt tttataaata aaaataatag
      781 gaacgcaaaa aaaagcgaat agcagtgcta atcgccaatc caatgaaatt gatttcctat
      841 tttttgcttt tttctgtagt tttaaatctt ctttactgaa ggaaactcgt tcaccataca
      901 ctaaaaagcg gtgtgagttg acaccttttg gtgtgcatgt cattaacgtg atcctatctt
      961 ggtctttttc aggcataaga taatctaaat aatttttttg taattcttct gctttaactt
     1021 cttcaattcg ataaatttga taggcatgtt gttcttcatt taccttaata aagaattttt
     1081 gccctttttt tactgaaggc aaatcatcga aaagattatc tttataaagt ccactgtgcc
     1141 ccgcgattaa aggattttta ccgtttcctc ctgtaatatc gccagttcct tccgtaatcc
     1201 ctacaccatt ctctaaaatt ttgtcggaag ttccgtcata aattggtagt tttaaagaaa
     1261 tacttggaat tgtaagaaca ccaacaggat cgcctaactc tattttcgca acatccaaag
     1321 aagaatctgt tttttcaaga ttttcaatgg ctaaaattct ttgctgttgt tgaaatttcg
     1381 ctacatttgt ttccacaact ggaattaaat agactgctac tgccgacagc aaccaaaaaa
     1441 atatcatcag tttgtgcatg actcttcttt ttgggggctt acgaataatc attagctgat
     1501 ccttctaggc tttcttctta aagtaaacaa ctgcagaaag tccccctgct cctagcaaca
     1561 agcaagagat caatacgtaa gattggaaac cgttgctacc agtcattggt acggaagctt
     1621 tttgattatt tttgatcgtg attcggtttt cttctttgta tgaatctttg tctactttga
     1681 aagagatctc ttttgttaat ttttgatagt tttcaggagc ttttgtttct tccagtgagt
     1741 aatctccttc agttaaacca gtgatttcaa aatgaccctc tttatctgat tttaggattg
     1801 ctgcatttgc tacatcgtct acccaaactg cttctttgac accatcttta tctgtatcag
     1861 taagtccacc gtagtactta ccttctgcat tcttagctgc aaattctgcg ccttcaagcg
     1921 gtgttgcttt gatacctgtt ccaaataggc cgctatcgac ttttaagaat tttgcgccat
     1981 aagtcacaac gtcagctttc gttttttcgt ggtgttcttc ctgattaaat ggattattgt
     2041 tataaacgaa atccacgcta tttgttaatg gatttgcgat ttcagcacta tcattcacaa
     2101 acatgttata agtcattttt agatcttgac catgtaaatt cgcaactgta tcacttactt
     2161 gtccattatt tacgataaaa tcgatcacaa aaccactatc agaattttcg attttatagt
     2221 cagtattttc ttttaatacg actgaaccat cattatttgt tactttaata tctgcttttt
     2281 cagcaacata agaaagtcct ttacctgctg tatctcccca tttaaataca ctgtaaggag
     2341 aagtttcgct tttcacaccg tttactgtga taccgtctgc gccacctgca atgtctgttg
     2401 gaatatgaat cgttccttga taatttactg cgtcaccaat ttggaaagaa tgttcttgtc
     2461 ctgttccatt tgttactgtt ttatcgatca ctggtgcttt atagtttacg tatgaagctt
     2521 ttaagtcact tgtcacgcca tcattcgtaa tttcaaaggt taatttattg tgcgcatcgt
     2581 tgcgagcgtc tgcgcctaat aaatattgat ctgctgttgg ttcttgatct gatcccacta
     2641 caacttccga aggaacttcc acaaaatagt attttcctaa cgtcaaatca gtcgcagtca
     2701 gctttccttg atcatccgat acaagatcac ccaattttgt tccttttcca ggttcgccct
     2761 tgtataactc aaattttgcc ccttttaaaa gggtttgttt gctttggtct gcgccatcgt
     2821 caccaaattt cgttaattca aaactcaact tagaaacaat gttcttagga tacaaatgaa
     2881 tattttttaa aaatcctgat cctgttttat ctgtcattgg cgcaatcaca accattggtt
     2941 ttgctttttg tgtgactaat ccagcagcgg atttactttc ataaaccaca tatacattcc
     3001 ctttggaatt gacataggct ggttgatcag taaaagtgat ctcaccattt tcatctacag
     3061 ctgaagtaat tttgttggtt gcattcttga cgtactcact gttagatccc ttgtcttcta
     3121 catcttgtac aatctcgtcc actttcgcgt tggttagatc agaatcttct ctcggtaata
     3181 cttgatccgt aatatttcct aacgtaaact caacatcacc ataatctgcc ttgttgtatt
     3241 tcgttacacc tgtaggatat tgatcgtgcg tataaccatc gtttttgatc ccgtcaatat
     3301 caacattcaa ttgcgttcct tgatcgtaca ttaatttatg aaccgtgata ttgaccttct
     3361 ctggtggtgt tttttgcgtg acatcttctg catgtacttg atacccctga aagcagcctg
     3421 caatgattcc taacattcca acaagcattc cagctttttt tacataccga tttattgcca
     3481 ttttttactc tcctcatttc atatttcaac gactcgcgtt gattaaaacc ttgttattgt
     3541 agctctccct gtaccacgta tctttctttt ccttgtgacg tataaacaca agtaaacaac
     3601 gttaataagg gtttctctcc tttctttggc aaagacaaga aagagttctc ttcaggaacg
     3661 ccatcgatta atttaaagta cccaggtacg ataaatttac gcgttacctt gtaacgatag
     3721 acgccatcgt tactaactaa gtccatcacc tcccctaatg acaacttttg aatatcagaa
     3781 aataaaacac ctggcatttc catgttatga cctgctagga catagtttcc tttgcccatt
     3841 tcggcatctt cataaaaata agtagccgct ccaagactca aagtgtattg atttgcccct
     3901 ttaaaaattg gcaatgctaa accaagtgca ggaatctgaa tttgtgcaaa tacttgttta
     3961 ttgacgccca tttcgattgc ttctttttgt atggacaaca tttgccccaa agacatatca
     4021 ttattgacgt tcgttgcatc ctccttataa ctctttgcat gtgcattgat ttgatctacg
     4081 ggcatctttt tttctatatt ttgcttaaac acctctcctt catgtttttg gtagctaaca
     4141 tagccaaaca cactgcaaaa gacgattaaa gtgactccta aaaaaccaaa taaccatttt
     4201 ttcatccatc tgcctccttt cagacaaaac aaaaaaactt tttctcattt ccatagaaaa
     4261 agttttaaaa atgaattcta taagttcgtc tcaactgttc aaagtttttc attgaaccta
     4321 acaaacaagc tttctctttt cctgttaact gcatatcatt gatatattgc gcgacagata
     4381 atcttttaag gtgtttttgc ttcatcgttt ttagtaactt ttcttccatt tctttcgaca
     4441 ctttttttct gttttcgatt tcttcataat tcatgagttt tcctaccttc cttgtttttt
     4501 gcaaaaataa aaaagcctat gatcacatac gctctacact tcagtcttgg gttatctttt
     4561 tctttcgttt aattactggc cgtgctggtt tcaaatcctt gattccatta tgggttctcc
     4621 aataatcgaa ttcttttcgc aatctttctt tttccatatc tgttccttta gataaggctt
     4681 tgaatacttc tttaggtgaa taaccacgag ctaacagctg tacatacctt ccttttgtca
     4741 aagtatccat atccatcgcc tctaaaactg gtcgcgccat cta
//
LOCUS       DACDIE010000114         4711 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.117, whole genome shotgun sequence.
ACCESSION   DACDIE010000114 DACDIE010000000
VERSION     DACDIE010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4711
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.117"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            106..357
                     /locus_tag="IV250_002682"
                     /pseudo
     CDS             106..357
                     /locus_tag="IV250_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            450..1244
                     /locus_tag="IV250_002683"
                     /note="aph(3')-IIIa"
     CDS             450..1244
                     /locus_tag="IV250_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAP8769915.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            1654..1765
                     /locus_tag="IV250_002684"
                     /pseudo
     CDS             1654..1765
                     /locus_tag="IV250_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787626.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            1786..1869
                     /locus_tag="IV250_002685"
     CDS             1786..1869
                     /locus_tag="IV250_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HAP8769916.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            1994..2731
                     /gene="erm(B)"
                     /locus_tag="IV250_002686"
     CDS             1994..2731
                     /gene="erm(B)"
                     /locus_tag="IV250_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAP8769917.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(2994..3857)
                     /locus_tag="IV250_002687"
     CDS             complement(2994..3857)
                     /locus_tag="IV250_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HAP8769918.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAI
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLEKELNRKVSGKEIQPTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTP
                     PIPKTPKLPGI"
     gene            complement(3859..4131)
                     /locus_tag="IV250_002688"
     CDS             complement(3859..4131)
                     /locus_tag="IV250_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP8769919.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            complement(4148..4363)
                     /locus_tag="IV250_002689"
     CDS             complement(4148..4363)
                     /locus_tag="IV250_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HAP8769920.1"
                     /translation="MIVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(4455..>4711)
                     /locus_tag="IV250_002690"
     CDS             complement(4455..>4711)
                     /locus_tag="IV250_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HAP8769921.1"
                     /translation="IGFVPYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKVS
                     TWSKNGITEHKGYDKKVLSMYKNVFFEMLERIIQLENEKE"
BASE COUNT         1446 a          796 c          865 g         1604 t
ORIGIN      
        1 ttcttgggag ttttgagact actatatgcg tttttttgtt gatttaccaa catgtttaga
       61 cattttcctc taggaaaaaa ttaactagca ccacgggtta tcttttatat agaagatatc
      121 gccgtatgta aggatttcag ggggcaaggc ataggcagcg cgcttatcaa tatatctata
      181 gaatgggcaa agcataaaaa cttgcatgga ctaatgcttg aaacccagga caataacctt
      241 atagcttgta aattctatca taattgtggt ttcaaaatcg gctccgtcga tactatgtta
      301 tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat
      361 gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa
      421 tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga
      481 aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg
      541 tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata
      601 aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc
      661 tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga
      721 gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga
      781 ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc
      841 cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc
      901 tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc
      961 tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc
     1021 tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga
     1081 gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg
     1141 atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt
     1201 gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta gatttagatg
     1261 tctaaaaagc tttaactaca agctttttag acatctaatc ttttctgaag tacatccgca
     1321 actgtccata ctctgatgtt ttatatcttt tctaaaagtt cgctagatag gggtcccgag
     1381 cgcctacgag gaatttgtat cgataagaaa tagatttaaa aatttcgctg ttattttgta
     1441 catttaactt gacggtgaca tctctctatt gtgagttatt agtggtacag ttttcaaccg
     1501 ttttaattat aaaaaagtgg tgcattttta aattggcaca aacaggtaac ggttattgca
     1561 ggtgtatttc ttatctatgg gtttaacatg gattttatca ttaaaatcat gagtattgtc
     1621 cgagagtgat tggtcttgcg tatggttaac cctaaagtta tggaaataag acttagaagc
     1681 aaacttaaga gtgtgttgat agtgcattat cttaaaattt tgtataatag gaattgaagt
     1741 taaattagat gctaaaaatt tgtaattaag aaggagggat tcgtcatgtt ggtattccaa
     1801 atgcgttatg tagataaaac atctactgtt ttgaaacaga ctaaaaacag tgattacgca
     1861 gataaataaa tacgttagat taattcctac cagtgactaa tcttatgact ttttaaacag
     1921 ataactaaaa ttacaaacaa atcgtttaac ttctgtattt gtttatagat gtaatcactt
     1981 caggagtgat aacatgaaca aaaatataaa atattctcaa aactttttaa cgagtgaaaa
     2041 agtactcaac caaataataa aacaattgaa tttaaaagaa accgataccg tttacgaaat
     2101 tggaacaggt aaagggcatt taacgacgaa actggctaaa ataagtaaac aggtaacgtc
     2161 tattgaatta gacagtcatc tattcaactt atcgtcagaa aaattaaaac tgaatactcg
     2221 tgtcacttta attcaccaag atattctaca gtttcaattc cctaacaaac agaggtataa
     2281 aattgttggg aatattcctt accatttaag cacacaaatt attaaaaaag tggtttttga
     2341 aagccgtgcg tctgacatct atctgattgt tgaagaagga ttctacaagc gtaccttgga
     2401 tattcaccga acactagggt tgctcttgca cactcaagtc tcgattcagc aattgcttaa
     2461 gctgccagcg gaatgctttc atcctaaacc aaaagtaaac agtgtcttaa taaaacttac
     2521 ccgccatacc acagatgttc cagataaata ttggaagcta tatacgtact ttgtttcaaa
     2581 atgggtcaat cgagaatatc gtcaactgtt tactaaaaat cagtttcatc aagcaatgaa
     2641 acacgccaaa gtaaacaatt taagtaccgt tacttatgag caagtattgt ctatttttaa
     2701 tagttatcta ttatttaacg ggaggaaata attctatgag tcgcttttgt aaatttggaa
     2761 agttacacgt tactaaaggg aatgtagata aattattagg tatagggcac ctctaataac
     2821 taccaattaa ttcacctcaa aatgaaatta gaaaaaaaca gcgaaatttt taaatctatt
     2881 tcttatcgat acaaattcct cgtaggcgct cgggacccca acatgaggaa atgtttttat
     2941 aaaaaaagag tagttaccgt tttttggtaa ctactctttt ccttttttaa ggtttaaata
     3001 cctggaagtt taggtgtttt gggtattggt ggtgtaggtg gctgcaagct ttccaatttt
     3061 tgttgaattg ctttaaattc aggtgtctct tggtgtttat ttagaaccat tttttgctct
     3121 attcgttcta aagtcggttg aatttctttc cctgatactt tacgattcaa ttctttttct
     3181 aaggtttctt ttggactaat agaaggtgtt tccaaacttg aatagagttt tactccttct
     3241 ctgttatata gccttatatc gctaaataag cccgttttat gaagggtttc taaattggtc
     3301 ggtaagtttt tgacaacaat atcatgcgct tgttttggtg ttgccctggc tgtcattgga
     3361 tcatctgcat acatggtttc atatcgttca attgttccta aatatgagtt gattttaggt
     3421 actgccatga catacatttt tgtttcataa cctttggctt gaagcattgt tgcggtttga
     3481 ataggaacgt ctgttgttcg tcctgtacct tcgatcacca aattataccc ttgatcgctc
     3541 aaacggctta tgatcgcttc tgtcatgcga ttagaataag gggtaacgtg ttttactacg
     3601 tctttttcat aaagtttcac tagttcatca aaattagggt gctgttgttt aaaggtatca
     3661 ttatcaatga caataacatt cccttgtgtt tcttcaaaaa ttgctgatcg caaactggtt
     3721 ttccctgacc ctggttgccc accaagtaaa aaagcggttg gcgattcaac cgcttttttt
     3781 ccttgaatca attcttctaa attatcattt aagcgattct caaattgttt gtcagtaaaa
     3841 ttgactatat ttgccatatt aagccacttt ctctttattc aaaacttgtt ttgagtaacg
     3901 attcattgac cgccaatact catgaacggc ttgtaattct tctaaagaat aatcaaaaag
     3961 atttacacgt tttgcaagct ttaattggtt taataaattg acttccaata attgagagtc
     4021 atttttattt aattcatggt tcaaaacata gtttaatact cgattataaa cgcttgccga
     4081 taattcgtta atgatctcta tttcaaatgt tttttcatac gtaactgcca tttcattttc
     4141 actcctttta aagtttgtca ggtaaatatt tccgtatata ttcttctaag gcttgatcca
     4201 taacttcttt tacgtttccg ccattctttg ctgtttcgat ttttatgata tggtgcaagt
     4261 cagcacgaac acgaaccgtc ttatctccca ttatatcttt ttttgcactg attggtgtat
     4321 catttcgttt tgctttttgt gcgcctaaat ttcccacaat cactcacttc tttctatttc
     4381 ttcttactct tattttatca tcaacaatca caaatcacaa gtgatttgtg attaatcact
     4441 tgtgatttgt gattctattc tttttcgttt tctaattgaa taattcgctc aagcatttca
     4501 aaaaatacgt tcttatacat ggataaaact tttttgtcat agcctttatg ctctgtaatg
     4561 ccgtttttag accaggtact tactttatta cttcgcttga taatattttg gaaaaccaag
     4621 ttatcttctt tatgttgctt atacagttct tccaggtttg attttatcgt tgcgctgtcc
     4681 gtatcaacta aataaggaac aaaaccgatc a
//
LOCUS       DACDIE010000115         4663 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.118, whole genome shotgun sequence.
ACCESSION   DACDIE010000115 DACDIE010000000
VERSION     DACDIE010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4663
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.118"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..867
                     /locus_tag="IV250_002691"
     CDS             <1..867
                     /locus_tag="IV250_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HAP8769922.1"
                     /translation="KKKKFSRSLLGILVLVAVGLSGKGYFQNNSTQEHDFQIKSSVQV
                     GNKADSESKEFLGIRYDPNKYPNNIVNINDGKYTLTKEDHEALSEKGSQTFWVNYADL
                     DQLGRAGNVEALITYDAVNSHSSKNVERPSFKSSVHIAGEYKDGVFDQERDTWVGKDS
                     NNEITQLEGYKGYLYNKSHSLAWSLGGDMEVHNLTLGTRAQNVGKNDQNGGMAYSETI
                     VRDAIYKNKNEKVLYKVDPIYKGNELVPRGSHVEAFSVNDNGKTVNLNVYVFNNQKGI
                     NINYQNGTWVRD"
     gene            887..1213
                     /locus_tag="IV250_002692"
     CDS             887..1213
                     /locus_tag="IV250_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769923.1"
                     /translation="METISINGLDKSVANRIKKGNGYYLLKKGKYSVARELETMSIVY
                     VAKNHFDVEQAIFDPDSEIGKCVELSEKTVSTKGAMIIEREMLDEIRVIIPKDTQMCE
                     DSISDD"
     gene            1247..2509
                     /locus_tag="IV250_002693"
     CDS             1247..2509
                     /locus_tag="IV250_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769924.1"
                     /translation="MSEKSIELKEYKRSDGEKYYPVANFSKSVYLVGRALVVSAIGIY
                     ILHWVTKVVQLLVDTLVSILVSLNDVNGTDDLGLKEMFKELLNAFFPNYLSYSYNVQS
                     FFAKLHLVDISILVLEAGTVFFVGYLVTLMFRHHYGEQRPFFDDMESRRLKREALEAM
                     DVKYDPNKIIGDETNNKKRKRNKTQLESDVLSTLKNIKISVHTRRDLSVNEDIREYIV
                     KIPQPKGKKVRETVLRQIQDLGSILTRATRGRAKFGEKEDEVSGDYFIFRASVNIERE
                     RKERVSTKNKETEKSTASIAVSNSQFAFPINLFADNSKNIQNQTEKARKYAETQKQSL
                     ELYLSSADVPASLTDMHVGNTSIEYLFTLSRTRKIQLSSMKEEIETYLHISGVIVRIN
                     AGRIQITLPLGENDRIPIDVPSMINSVF"
     gene            2589..3965
                     /locus_tag="IV250_002694"
     CDS             2589..3965
                     /locus_tag="IV250_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAP8769925.1"
                     /translation="MAMKLLEKVKNRAEKAESILLDKEPNIKLNGKFSVNMLNTKAKD
                     PTDAIFGIKPDGTIAHFCISDTPHMLVAGTTGSGKSVLLNEILVTAICHAMPDELKLG
                     IVDPKRVEFGRYKKLPYMLADPITDMDEAYEFFEYLVILMNERYKLMEKAGVQNITQY
                     NKYAEKHGIEKFPYVILLVDEYSQLVGTHKEVEGLIVQLGQMARAAGIHVILATQSPR
                     STVVTGIIKANFPSRVCLMVASDLESRIVLDEGGGESLSPKGDMFIKLNGGNKIRAQG
                     AYISNEEIEAIFDHLRNTMPEPEYVDYRAIVAESRGEGGEDEDFGGIGKAPVRKSAKR
                     NLNTPLANSSSKEPSIEKKSTFALKLEKNKEEKEKQGAKTLEKLQAEKLEKVLKALDK
                     VDPEPQTSNNTNNEQPISSKTKPKTTDPKVSVKVTITTDNSEKKQVNGTTSPIMQKML
                     KAKKQVHK"
     gene            3962..4591
                     /locus_tag="IV250_002695"
     CDS             3962..4591
                     /locus_tag="IV250_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769926.1"
                     /translation="MIIVSSFLRWLTPYKYTDEVVVKRAKKKDKKAVLTPKQMLDHGI
                     LGVKVLEVNKPFESNSAREKLLGAEADVAYFYHDKSKHQIAIKFNPKSAWRYQARDDL
                     KRRKLRPMLYPEGKEAFDRTHIVPIGFHGTESDNRLLVGWNSNQNRVQINKFEKKVRK
                     FNERKTILWFADICKQPDGTAKWEAVIFDENGKKVMSETWHDKAKFVWQ"
BASE COUNT         1797 a          684 c          965 g         1217 t
ORIGIN      
        1 aaaaagaaga agtttagtcg atcgttgtta ggaattttgg ttttagttgc ggttggtctt
       61 agtggcaagg gttactttca gaataacagc actcaggaac atgattttca gatcaaaagt
      121 agtgttcagg taggtaataa agcagacagt gaatcaaaag aatttttagg gatacgttat
      181 gatccaaaca aatatccaaa taatatcgta aacattaatg atgggaaata tacactgaca
      241 aaagaagacc atgaagcatt atcagaaaaa ggcagtcaaa ccttttgggt aaattatgca
      301 gatttagatc aacttggaag agcaggaaat gttgaagcgt tgatcaccta tgatgctgta
      361 aattctcact catctaaaaa tgtggaacgt ccatctttca aaagttctgt ccatattgca
      421 ggagaataca aggatggagt atttgatcaa gagagagata cttgggtcgg caaagattca
      481 aacaatgaaa tcactcagct agaaggctat aaaggttatc tctataacaa aagtcattca
      541 ttagcatggt cgctaggagg agatatggag gtacacaatt taacacttgg aacgagagcg
      601 caaaatgttg ggaaaaatga tcagaatgga gggatggctt actcagaaac gattgtacgt
      661 gatgcaatct ataaaaataa gaatgaaaaa gtattgtata aagttgatcc aatttataaa
      721 gggaatgaat tggttcctcg tggttctcat gtcgaagctt tttcagttaa cgataatgga
      781 aaaacggtaa accttaatgt ttatgtattt aacaatcaaa aaggaatcaa catcaactat
      841 caaaatggta cttgggtaag agattagaaa ttgagaaggt gaagaattgg aaacaataag
      901 catcaatgga ttagataaat cagttgcaaa tcgaataaaa aaaggaaatg gctactatct
      961 tctgaagaag ggtaaatatt cagttgctag agagttagaa acaatgagta tcgtgtatgt
     1021 agctaagaat cattttgacg tagaacaggc tatttttgat ccagattctg aaatagggaa
     1081 atgtgttgaa ttatcagaga aaactgttag tacaaaaggg gcgatgatca ttgagagaga
     1141 aatgttagac gaaatcagag taatcattcc taaagatacg cagatgtgtg aggacagcat
     1201 aagtgacgat taaccaaaat cagaaagaaa ataaggagat attcagatga gcgaaaaatc
     1261 cattgaatta aaggaatata agcgaagcga tggagaaaaa tattatcctg tagccaattt
     1321 ttctaaatca gtatatctgg ttggacgtgc attggtagtt agtgcaattg gcatatatat
     1381 tttgcattgg gtaacaaagg ttgtacaact gcttgtagat accttagtaa gtattctcgt
     1441 ctcgttaaat gatgttaacg gaacagatga tttaggatta aaagagatgt ttaaagagtt
     1501 actcaatgcg ttttttccaa attatttaag ttattcatat aatgtacaaa gcttttttgc
     1561 aaagctacac ttggtagata tctctatttt agtattagaa gcaggaacgg tattctttgt
     1621 cggatatttg gttactttga tgttcaggca tcactatgga gaacaaagac cattctttga
     1681 tgatatggaa agtagaaggt tgaaaagaga agctctagaa gctatggatg tgaagtatga
     1741 tccaaacaag attataggag atgaaacgaa caacaaaaaa agaaaacgaa acaaaacgca
     1801 attagaatcc gatgtgctat caacgcttaa aaatatcaaa atttctgttc atacaagacg
     1861 tgatctaagt gtcaatgaag atattcgaga atatatcgta aaaataccgc aaccaaaagg
     1921 gaagaaagtt agagaaacag ttttacgaca aattcaagat ttagggtcga tcctgacaag
     1981 agcaacaaga gggcgagcta aatttgggga aaaagaagac gaagtttcag gtgattactt
     2041 catcttcaga gcatcagtaa acattgaaag agaacgtaag gaaagagtaa gtaccaaaaa
     2101 caaggaaacg gaaaaatcaa cagcatccat tgcagtttca aattcacaat ttgcttttcc
     2161 aatcaatctc tttgcagata actccaaaaa tatccaaaac caaaccgaaa aagctagaaa
     2221 atatgccgaa actcaaaagc aatctttaga actttatcta tcttctgctg acgtacctgc
     2281 aagtctaaca gatatgcacg ttgggaatac atctattgaa tatttgttta ctttatctcg
     2341 taccagaaaa attcaactaa gctcaatgaa agaagaaatt gaaacttact tgcatatatc
     2401 aggtgtaatc gtgcggatca acgcagggcg tattcaaatt actttaccat taggagaaaa
     2461 tgatcgtatt ccaattgatg taccatcaat gatcaatagc gtgttttaga agacatcaca
     2521 aatagaaaca gaaaatacaa actaccagtt cacaaacaga aaagtaccga gaaaagagag
     2581 gaatagaaat ggctatgaaa ttgttagaga aagtcaaaaa tagagccgaa aaagctgaat
     2641 ctattttgct agataaagaa cctaatataa agcttaatgg aaaattcagt gtcaatatgt
     2701 tgaacacaaa ggctaaagac ccgacagatg ctatttttgg aattaagcct gatggcacaa
     2761 ttgctcattt ttgcatcagc gatacgccac acatgttagt tgccggaaca acaggatcag
     2821 ggaaatcggt attactgaat gaaattcttg ttacagctat ctgtcatgca atgcctgatg
     2881 aattaaagct agggatagta gacccaaaac gagtagagtt tgggcgttat aaaaaattac
     2941 cctatatgct tgctgatcca attactgata tggatgaagc ttatgaattt tttgaatacc
     3001 ttgtgatctt aatgaatgaa cgctacaaat tgatggaaaa agcaggcgtt caaaatatca
     3061 cacaatataa caaatacgct gaaaaacatg gaatagagaa atttccctat gtgatcttat
     3121 tggttgatga atattcgcaa ttagttggaa cacacaaaga agttgaaggg ctgatcgttc
     3181 aattaggaca aatggcaagg gcggcaggaa ttcacgttat tcttgcaaca caatcacctc
     3241 gatctacagt cgtgacaggt attattaaag caaatttccc atctagagtc tgcttgatgg
     3301 tagcaagtga tttagaaagt aggatcgtat tagatgaggg tggtggagaa agcctaagtc
     3361 caaagggaga tatgtttatc aagttgaatg gaggaaacaa aatcagagcg caaggagcct
     3421 atatcagcaa cgaggaaatt gaagcgatct ttgatcacct tagaaatact atgccagaac
     3481 cagaatatgt tgattaccga gcaatcgtag ctgaatctcg tggtgagggc ggtgaagacg
     3541 aggattttgg tggaattggt aaagctcctg taagaaaatc tgctaaaaga aatttgaaca
     3601 cacctttagc aaattcatca tctaaagagc catcaattga aaagaagagt acgtttgctc
     3661 taaaacttga aaaaaacaaa gaagaaaaag aaaaacaagg agctaaaaca ctagaaaaac
     3721 ttcaagccga aaaattagaa aaagttttaa aagcattaga taaagttgat cctgaaccgc
     3781 aaacctcaaa caacacgaat aatgaacagc cgatttcatc taaaactaaa ccgaaaacta
     3841 ctgatccgaa agtttcagta aaagtgacca tcacaaccga taattctgag aagaaacaag
     3901 taaacggaac tacttcacct atcatgcaaa aaatgttaaa agccaaaaaa caggtgcata
     3961 aatgatcatt gtcagttctt ttttgaggtg gcttacgcca tataaatata cagatgaagt
     4021 agttgtgaaa cgagctaaga aaaaagataa aaaagcggtg ttaactccta aacagatgct
     4081 tgatcatgga attttgggtg tgaaggtttt agaagtgaat aaaccgtttg aatcaaatag
     4141 tgcaagagaa aagttgcttg gcgctgaagc agatgtggca tatttttatc atgacaaaag
     4201 caagcatcaa atagctatta aattcaatcc aaaatcggca tggcggtatc aagcaagaga
     4261 tgacttgaaa cgtaggaaac ttagaccaat gctctatcca gaaggaaaag aagcatttga
     4321 tagaacccac atcgttccca tcggttttca tggtacagaa tcagataatc gcctattagt
     4381 aggttggaac agcaatcaaa atagagttca gatcaataaa tttgagaaga aagtaagaaa
     4441 attcaacgaa cgaaaaacaa tcctatggtt tgcagatatt tgtaaacagc cggacggaac
     4501 agcaaagtgg gaagcggtga tatttgacga aaatggtaaa aaagtaatgt ctgaaacatg
     4561 gcatgataaa gctaaatttg tttggcagta gaggtgaaaa ttgtgttaac tattctaaaa
     4621 ctatatagag agggatttaa cgaaaaagta agtattggtg aat
//
LOCUS       DACDIE010000116         4461 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.119, whole genome shotgun sequence.
ACCESSION   DACDIE010000116 DACDIE010000000
VERSION     DACDIE010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.119"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            574..1008
                     /locus_tag="IV250_002696"
     CDS             574..1008
                     /locus_tag="IV250_002696"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769927.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRY
                     NHNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSA
                     VDAMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
     gene            2298..3221
                     /locus_tag="IV250_002697"
     CDS             2298..3221
                     /locus_tag="IV250_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP8769928.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEII
                     FSFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDK
                     QLVIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKE
                     KFFDLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFT
                     WKAEINHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQ
                     QQQTEEKQAEKRELSNKLKQDLLGNLQNLFD"
     gene            3237..3776
                     /locus_tag="IV250_002698"
     CDS             3237..3776
                     /locus_tag="IV250_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP8769929.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINE
                     TGQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQ
                     EKDNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNT
                     QPLIEKKWWQIWKSNRTNL"
BASE COUNT         1664 a          667 c          758 g         1372 t
ORIGIN      
        1 aaaaaaaact gtgttggtac tgggttctat ttctctagga atattaggtt ttagccccat
       61 gctattatcc gtaattggaa aaaacaattc caacagagaa cttaaattga attcaaatta
      121 ttacaacagt cctaacgaag aattgtttgt aaaagaaacg gataaataat aaaaaagact
      181 gctcttccga gcagccagtt aaaaaataat gagtgtttat tcttgaaaaa gcaacgcgct
      241 tataaagcgc taccaaaaat ttatcatatt ttatgaaaaa taacaatcca gtaaaaaatg
      301 actggtaaaa attcacaaag aaacggaaag aagcaactgc agaaaatcaa gccttaaaaa
      361 gagcttttta cctagaccaa ttaaaaatca caaagtgagc ccctgtatcg gttgatacaa
      421 gggctacttg gcatactgta cattatgtct actaaccttt aaaataacac gttttataaa
      481 ataaaccctc ttgacaatat ttaattttct gattattcta aatatataca gaaagcgctt
      541 tcctaggatt ttatatctaa ggaggaagta acaatgaaaa ataaaattat taaaattatt
      601 acttgtctat ttttgttcgg tatgattgtt ggagcaagta ctccgttaaa tctgtttcct
      661 tttagtgcta gaggtataaa agtggaagct gcaagttcac gatataatca taatcatcgt
      721 ggatttactt gctataaaat gaactattat gttactaata aaatgtgtag agatttgaaa
      781 aaaaattata ataagctaaa gactcctgct caaattgcat cttttattcc tattggtgga
      841 gttggaacat gggttattac aaatgctttt agtagtgctg ttgatgcaat gaatgtcttt
      901 gtaagagctg caaatcaagg aaaaggtgta caattaacat ataatgctca ctttagcaat
      961 acaacatcat atcaatacaa tgattacgct cgttatgtaa taaaataatc agaaagtgaa
     1021 actatctcat gaataaaaag attttagctg taattatttc aataatagtt ttaatcacta
     1081 tgttcttcat atttaggcta attttcaata taagcttaca aaaaagtatt ctttatctaa
     1141 ttcctatagc atttatctta gcaattttta gaagtattta cggaaataaa aactctaaat
     1201 agctaaaaat aaatcatact aaattttaca aaaagtggct agcattttct agccactttt
     1261 tgtattgtgg agcgtgggtt ttgaggggga ttcaaggggg agcttgcgcc cctttgacaa
     1321 gcggttattt gcaccaaata cggaggtatt tggctagcaa atatagagct tgccaaaccc
     1381 ttttagtgag ttttgtagaa agtaccttta aaggtacttt cacattacga acgatacgtc
     1441 cgtaggacgt actgaaaggg tgggacttat gagcaaatta atgcaaaatt tggctagcaa
     1501 aaaagctaaa aaatatatgc taataatcga gcgaaataaa cactttttca agtgagcgaa
     1561 tccgccatga caaagctgaa gcagtttatt tcagctttgt catggcgtgt gccgactttt
     1621 ttaaaagtct aggcacacga gcaaatatct ttatcaagct ctaatttgcg ttttaagacg
     1681 tttgaaacgc ctaagtatat aaatatataa gaataggttt aagagtgctt aaatcgaatt
     1741 ttagagggca tttagacaat tttagggttg ttagggcttg ccctgaccgc ctgtaaggcg
     1801 ttttaagaac aagataaggg atttatacta ggactgtctt aaaatgtctt aaacgaacgc
     1861 tcagagcttc tcaaagggtt ccttatgaac ggcttttagc aaaggagccg gaggcgactg
     1921 cgcagtataa tgtgagagca gagcgatcac acctttttag cgaaccgtag ggagcaaggt
     1981 ttggaattgg cagttttgat aaattcgcga acctgtttag agaaaaaagg agtgattttt
     2041 taggcgggaa aattagcctc ccctcttttt atatctcttt ttatatctct ttttatatct
     2101 cttttatgtc ggctgtgtcc ttactctccc aagcaattca gagttttttc aagtaggttt
     2161 tctacgcttt tcaagtaggt tttctacgct tttcaagtag gttttctacg cttttcaagt
     2221 aggttttccg acattattga gtatttttat aaagtatgtt acttttattt catactttat
     2281 aaaagtagga gaagcttatg gcaaatgaaa ttgtaaaata tgaaaataga ttaaatcata
     2341 ttcctctgag aaaatttaat agtcgtgaaa tgaacttatt tttttcaatt gcttcacgtg
     2401 tacgtgaaaa aggtgtagat gagattattt tttcatttca cgatttgaaa aaactaagcg
     2461 gttatacaga acgtggggaa caatttataa aagatcttag tagcacgtat gacaaattat
     2521 taagtattaa tgcttggaat gatgatggaa atactttaac taaatttgtt gctttcacaa
     2581 aatatagcat tatacgagat aaacaacttg ttataatttc agttaatcca gaatttaaag
     2641 gactttttaa tcaattaaag aactggacaa gatttaatct agagcaattt actaatttgg
     2701 atagcacata ttcaaaaact atgtttcgat tattaaaaca atatcgaact caaggctgga
     2761 ctgaattttc caaagaaaaa ttttttgatt tactagatat tcctaaaagt tattggaata
     2821 gtccgtcaaa tattgacaaa tttatcttaa ggccaattaa agaagaactc actcccttat
     2881 ttaaaggatt aactattcgt aaaaaatatg gtaaaggtcg aggtaaacct gttatcggtt
     2941 atcgttttac ttggaaagct gaaattaacc atgctgacga tttttccaaa gggaaacaag
     3001 aagatttacg tataaaatta tttaatattg atcacaatgg agaattaaca cgagaagaaa
     3061 aatggcgcgc taaagatcga atactaaatc ttcccttagg cactcatgaa gctgatttta
     3121 aacaacaaca acaaactgaa gaaaaacagg cagaaaaacg agaactttca aacaaactca
     3181 aacaagattt actaggaaat ttacaaaact tattcgatta agcttaagga gtatttatgg
     3241 cagataaaac aatcaaagaa cttgcggaag aattaggagt tagtaaaaca gcaattaaca
     3301 aaaaagtttc tgacaaaaat cgaaaacttt ggtttgcaaa aaacggaaac aagtttgtga
     3361 ttaacgaaac cggtcaaaaa gctataaaat caatgttttt agtcgataac gcaaacttaa
     3421 atcgaaaact agttggcgaa aaatcaaaaa ccaaaaacca taaaaacacc aaccaagttt
     3481 ccgaattaaa actcctttct ttcctagaaa aacagttaaa tcgaaaagat atacagctac
     3541 aagaaaaaga taatcaaata aaacaaatgc aaaaattact cgaccagcaa caacaactca
     3601 ctctacaagc gaaccaacaa attgagaacc tacaagaaca gctccattta acttatacag
     3661 aagaaactcc aacaaccgaa tcaactgcct tttctgaaaa aaaagcagac aatacacaac
     3721 cacttattga aaaaaagtgg tggcaaattt ggaaatctaa tagaaccaat ctctaaaatt
     3781 ttctagaagt tagttcggat agtagaggaa aaagttgtac tacctgatgg ggagatcact
     3841 caaattttta tgagaaagaa aaaatagttt acaactgtaa acaatcaatg tttatagttg
     3901 taaactatca aactatatgg tgacttattg cttaaaatag gttgaaatct cactcaattt
     3961 ttggtggaaa aacaaaataa attctaatcg attttgttga aaaagtcata aaaaaacaaa
     4021 tgtgatttta acaaaatttc aaaaaaatcg tttgaaattt cgtttatttt ttttgagaat
     4081 tttgatcatc gtagggtcat tgtagaatca ttatgagatc atcgtgggat catcgaataa
     4141 agtatgggaa aggcttgcgg tgcttggtat taagggcatt ctccgcctcc caggttcttg
     4201 accattttct cttataagtg ggatttttac cacgttgaga gaaaatggtc aagaacctgg
     4261 gaggcggaga aaacatacca agcaccacaa gcaaaaaaag ttagagtaac ttccaaaaaa
     4321 gaaaaatttt ttagaacaac ttttgaaaat ttaattttga tttatatccg ttcaatttaa
     4381 acatttgata aactgaaggc tagtatttaa aatgtgtata caagtggtag cgttaaaaaa
     4441 agtggaggta taacctatgt t
//
LOCUS       DACDIE010000117         4453 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.120, whole genome shotgun sequence.
ACCESSION   DACDIE010000117 DACDIE010000000
VERSION     DACDIE010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.120"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(150..1670)
                     /locus_tag="IV250_002699"
     CDS             complement(150..1670)
                     /locus_tag="IV250_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAP8769930.1"
                     /translation="MTKMESELLVQIKQLTQLLEIQQQENTLLREQIAEMNRRLFGRK
                     KETPPVDGQIDLLDDSTFNEPEHTGQESQEPITVSSFKRRKRKGLKALSLEGLPEVPI
                     HYELKGADCLCEDCGHTLHDIGATRLREEPLYVPAHIEKQVIYQHAYHCRECEALGET
                     KIKKAPVPKPLINNSLGSSSIVTQSIWEKFVKKVPAYRQEKEWHALGFPLDRKKITNW
                     HIKVSEMVLEPIYNCLHQELSNQEVLHADETSYRVLNTHKEKTFYWLYASGKSEEKQV
                     VLYEHANSRSVDEPKSFLESFSGFLHTDGYAAYGKLPVNNVYCWAHVRRKFFEALGKQ
                     PSPKSHAKIGLDFCDKMFRIERTFNKLSAEERFVARKETLQPVMTEFFHWCESFAYLP
                     KSQLGKAVQYAVNHKDGLQIVLLDGRLELSNNRAERAIKELVIGRKNWLFSKSLKGAR
                     SNGIILSIIQTAVANGLNIRKYLNHLFTEIPNLSSMTPEALRAYLPWNQQIQEICK"
     gene            complement(1913..2098)
                     /locus_tag="IV250_002700"
     CDS             complement(1913..2098)
                     /locus_tag="IV250_002700"
                     /inference="COORDINATES: protein motif:HMM:NF017528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HAP8769931.1"
                     /translation="MPMLIDATALKHIFIVCGKTDLRKGIDGLAAFVIEEYDLDVYDQ
                     ALFLFCGTKKDRFKALY"
     gene            complement(2082..2264)
                     /locus_tag="IV250_002701"
     CDS             complement(2082..2264)
                     /locus_tag="IV250_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769932.1"
                     /translation="MNKKDIVPVQFSSAAQKSQIDTKPKLACRIKSEDKEIFIYNGMD
                     KYLLYTLLKELNANAH"
     gene            2505..2723
                     /locus_tag="IV250_002702"
                     /pseudo
     CDS             2505..2723
                     /locus_tag="IV250_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            3218..3817
                     /locus_tag="IV250_002703"
     CDS             3218..3817
                     /locus_tag="IV250_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8769933.1"
                     /translation="MKTVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLADVLEISRGRVVVKDRLAMKEQKTAKHNSVYISNKLRKTILEYVQQEFPDQ
                     LLQQDFDGYLFPSRKGTNEPLNRQSLWRIIHDAGTAIGLKDIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            3881..>4453
                     /locus_tag="IV250_002704"
     CDS             3881..>4453
                     /locus_tag="IV250_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769934.1"
                     /translation="MSTLYIIGNGFDLSHGLKTRYTDFSKWVEKSHSEIFDKIDTMIN
                     ENNEVRSTADDKIGWFNFESSLHELIQSPVFEDYVEESKDYLIGYGHEDWSDSAHHDF
                     QNMVYDYVVSLEELQQLFKEWIRKEIQPTIEHKIPKYQFSSDVLFFTFNYTDVLEILY
                     KQPKENIYHIHGSYDDIIIGHSENFDNFIGD"
BASE COUNT         1421 a          803 c          792 g         1437 t
ORIGIN      
        1 attattcagc aaatatgaga atggtttttt tatttgatta gacataactt aataattcag
       61 tagttttgag cgtaagcgtg tcatagaatg gtacctaaac acaaatagcc cgatttgata
      121 cactcaaatc aggttatttg tggcttatat tatttacaga tttcttgtat ctgctgattc
      181 catggcaaat aagcgcgtag cgcttcaggt gtcatcgaag acagattggg aatctctgta
      241 aaaagatgat ttaaatattt gcggatgttt aacccattcg caacagctgt ctggataata
      301 ctcaatataa ttccatttga tctagcgcct tttaagcttt tagaaaatag ccagtttttt
      361 cgaccgatca ctaactcttt gatggcacgc tctgctcggt tatttgaaag ttccaagcgt
      421 ccatcaagca acacaatctg aagtccgtct ttatgattga ctgcgtattg aaccgccttt
      481 ccaagctgcg attttggcaa ataagcaaaa ctctcacacc aatgaaaaaa ttcagtcatc
      541 acaggctgca acgtttcctt cctcgctaca aacctttctt ctgcgctaag cttattaaag
      601 gtccgttcga ttcgaaacat cttgtcacag aagtctaatc ctattttggc atgacttttt
      661 ggggacggtt gtttacccaa tgcttcaaaa aactttctgc gaacatgcgc ccaacaataa
      721 acattgttta ccggcagctt tccataggcc gcgtaaccat cagtatgcaa gaaaccgcta
      781 aatgattcta aaaaactttt tggctcatca acggaacgcg aatttgcgtg ctcgtagagc
      841 acaacttgtt tttcttcgct tttacctgaa gcatataacc agtaaaaggt cttttcttta
      901 tgagtattca atacccgata gcttgtctca tccgcgtgta agacctcctg attgcttaat
      961 tcttgatgaa ggcaattgta gattggctct aaaaccattt cacttacctt gatatgccaa
     1021 ttagtgattt ttttacgatc aagagggaaa ccaagtgcat gccattcttt ttcttgtcga
     1081 tatgccggca cctttttaac aaatttttcc caaatactct gtgtcacaat tgaagaggaa
     1141 cctagactat tattgatcaa cggtttagga acaggcgctt ttttgatttt tgtttctcct
     1201 aatgcttcac attctctgca atgataggca tgttggtaga tgacttgttt ttctatatgg
     1261 gcaggaacat acaatggctc ttctctaagc cttgtcgcac cgatatcatg taaagtatga
     1321 ccacagtctt cacacaaaca atcggcacct tttaattcat aatgaatagg aacttcaggc
     1381 agaccttcta atgaaagtgc tttgagtccc ttgcgttttc tgcgtttaaa agaagatacc
     1441 gtaatgggtt cctggctttc ttgtccagtg tgctctggtt cattaaaagt tgagtcgtct
     1501 aataaatcga tttgcccatc tacgggtggt gtttcttttt ttcgtccaaa caagcgtcga
     1561 ttcatttcag caatttgttc tctaagaaga gtattttcct gttgctggat ttcaagcaac
     1621 tgtgttaatt gcttgatttg tacaagaagt tcagattcca tttttgtcat ttttacacca
     1681 ccattctagc gattttctta catctattat accattttat gacaacgaaa aacagacaaa
     1741 atccttgatt taacaaggtt ttcgtctaat atatcgcacc ttttttcgtt tcttttagcg
     1801 tacatttttc ttcgattgaa aggccagtca gtaagacttc ttttctggaa cgcggccatt
     1861 gaaggtaccc actttcaaat cttttgtaca ttaatacaaa tccattttga tcctagtaaa
     1921 gagccttgaa gcgatctttc ttcgtaccgc aaaataagaa aagcgcttga tcgtaaacat
     1981 caaggtcata ttcttctata acgaaagctg ctagtccatc aattcctttt cgaagatctg
     2041 tttttccaca aacgataaaa atatgtttca atgcagttgc atcaatgagc attggcattc
     2101 aactctttta acaaggtata caaaagatat ttatccattc cattataaat aaatatttcc
     2161 ttatcttccg atttgattct gcaagctagt tttggcttgg tgtcaatttg cgatttttga
     2221 gccgcacttg aaaattgaac tggtacaata tcttttttat tcattcgttt ttctccttct
     2281 atatttgata gtttaattat agaagaaaga taatcggcgt acgagatact atcctatgac
     2341 acgcttacat ttaatttaat attttgcctc aaattagcca cactttttat agaactaaga
     2401 ggaaaaatga agttttcttc ctattaatat agttgtttga aatcttaaat atagcgtttt
     2461 acgatgtaat gggaagtatg gtgaataaat caactatgtt cgtacgaagc atttactcaa
     2521 ctaatttaat cgaatctttc aataaacaaa tcaagaaata cagccaccgc aaggaacagt
     2581 tccaaaatga agagtcgatg gaacgtttct tagtctcgtc ttttgatact tacaaccaaa
     2641 aattcctagg tcgcagtcat aaaggtttcc aacaggccga aggcgagctc gaacaaatgc
     2701 tgagtcaacc gatggagaat tagaaacaat ctacagtagg gaagaaccat tgacacaaaa
     2761 ttattgacgc tcccaactaa aagtgtcaaa ataatgatga aatcatttag ttcttataaa
     2821 ttagaaaaga gaaaataaat attccatatc tactttattg gatccgtaac atgactaaat
     2881 tctgaatact ttttaaagct tatcatttgg taaaactgaa tataagttaa ttatatagac
     2941 tgaaaatttt gaattttcag aatagaataa cttaaaagta ctgacaaaat tttttaaagg
     3001 ggcaatatta atgttctgag ataatcaaat cctaccgaaa atatcaaaaa tccaatgtga
     3061 cacaatcaga ttgtgtctca ttcgagggaa tcacacaatc ccacgtggct ctaggcagaa
     3121 ttgaattttt gcttggggct ttttccttat attgattttg aatgagactt attatatgtt
     3181 ataatgcaat taagtatcat tggagagggc gaagaaaatg aagacggtag aacctattcg
     3241 agacaaaaaa aagatcgatg cgatgaaggc aatcttggct tcgggaaaat atggccaacg
     3301 aaatttggtt cttttttcga ttgggatcaa caccgcctat cgcatttctg atttgcgtca
     3361 attgaagctg gccgacgtgc tggaaatttc acgcggtcgc gtggtggtca aagatcgatt
     3421 agcgatgaag gaacaaaaaa cagctaaaca taattcggtc tatatttcca acaaattgcg
     3481 aaaaacgatt ttggagtacg ttcaacagga atttcctgat caactccttc agcaagattt
     3541 tgacggttat ttgttcccta gtcgaaaagg gacgaatgaa ccgctcaacc gccaaagtct
     3601 gtggcgaatc attcatgatg ctggcacggc aatcggattg aaagacattg gcccacattc
     3661 catgcgaaaa acgttcggtt actttctcta taaacagggg accaaaacag aaatcattca
     3721 gtccttgttg aaccattctt ctcagcgcga aacattgcgg tatattggta ttacacaaga
     3781 agacaaagat acagctgtaa aaagcttaga tttataatat aaaagttaag tttaaacatg
     3841 gatagtttta aattagtaga ttgagtgttg ggaggaaaga atgagtacat tatatattat
     3901 aggaaacggt tttgatttaa gtcatggttt aaaaactaga tacactgatt tttcaaaatg
     3961 ggttgaaaaa agtcatagtg aaatattcga taaaatagac acaatgataa atgaaaataa
     4021 tgaggtgaga agtacagctg atgataagat aggttggttt aattttgaaa gtagtttgca
     4081 tgagttaatt caaagtccag tctttgaaga ttatgtagaa gagtctaagg attatctcat
     4141 aggttatgga catgaagatt ggtcagattc tgcccatcat gattttcaaa atatggttta
     4201 tgattatgtt gtttctctag aagaattaca acaacttttc aaggaatgga tacgcaaaga
     4261 aatacagccg acaatagaac ataaaattcc aaaataccaa ttttcgtccg atgtactatt
     4321 ctttacattc aattatacag atgttttgga aattctatac aagcaaccga aagaaaacat
     4381 atatcatatt catggcagtt atgacgatat aataatcggc cactccgaga attttgacaa
     4441 ctttatagga gat
//
LOCUS       DACDIE010000118         4433 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.121, whole genome shotgun sequence.
ACCESSION   DACDIE010000118 DACDIE010000000
VERSION     DACDIE010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.121"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(543..2048)
                     /locus_tag="IV250_002705"
     CDS             complement(543..2048)
                     /locus_tag="IV250_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8769935.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDN
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="IV250_002706"
     CDS             complement(2045..2731)
                     /locus_tag="IV250_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8769936.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="IV250_002707"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="IV250_002707"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAP8769937.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1240 a          919 c          729 g         1545 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgcat tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat att
//
LOCUS       DACDIE010000119         4376 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.122, whole genome shotgun sequence.
ACCESSION   DACDIE010000119 DACDIE010000000
VERSION     DACDIE010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.122"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(83..1051)
                     /locus_tag="IV250_002708"
     CDS             complement(83..1051)
                     /locus_tag="IV250_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAP8769938.1"
                     /translation="MSKNGIIVNEWEKLTALSNRRLNVQNMYTPLKMQWSIDRITIVG
                     KLKENIYYHTQNDVLILDFEQLMRVNEGNGYLKAVGNNGWQLLDQYEENIAYIEILKW
                     QDGKGRIDFNPNKIGHFLASSMKNFIHDLFLEPHFSRADIACDIIDVPDEFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRLYNKKLEQETKRKIVPPEIETWWRLELQ
                     LRRGKATDWHAMVHESLNSFASPHFLPLDVKTNDKVMIFGLIANPEMWGFLERRMKYK
                     LRDILKRESQNDELTNHLRETFSESADVLKKELDTWLQGLDVTEEE"
     gene            complement(1501..2907)
                     /locus_tag="IV250_002709"
     CDS             complement(1501..2907)
                     /locus_tag="IV250_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAP8769939.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDSQKTNDWHKHIDFTKSDEN
                     IYLEQTNLADKYEELFGEVVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIG
                     ILDDFQTNRADGSSTGISKQQAEQNRVIANKILVQYYKEFQERNPNLAVYNAVIHNDE
                     ISPHLHLNIVPVAEGYKRGVQKQPSFNKALVQQGIHADKDNGRVLFNNFRNQEVDSIE
                     RLMNDYGWKRDIVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSDELRYIYRQREE
                     NERDKALMASERAKIEEEKKNMLNVKKEMAVIFQKDMVPLESFKRLPDGSYHLSGNDF
                     VDLYQRATRATNAKRNADNMSEQYLQMLEQNTELNEYVATLEEERHLSGRKQLADLQV
                     ENKKLKEENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDIGLSEELEVLEQEKTLFY
                     RQNVSQHKLERSEDEIEW"
     gene            complement(3129..3490)
                     /locus_tag="IV250_002710"
                     /pseudo
     CDS             complement(3129..3490)
                     /locus_tag="IV250_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3494..3802)
                     /locus_tag="IV250_002711"
     CDS             complement(3494..3802)
                     /locus_tag="IV250_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769940.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLNLPDEDNYSEMLERMMDVL
                     NSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQISVINSKEKEM
                     "
     gene            complement(3811..4230)
                     /locus_tag="IV250_002712"
     CDS             complement(3811..4230)
                     /locus_tag="IV250_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769941.1"
                     /translation="MTDLPEKMTIKNLADELGISKNTVRNQLVNAGYDLARYKDNGVI
                     MLDKELIGVVRSIYRSKGGQLTGDLPVNDAGNDFPLEKENEFLREQLAEKDRHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(4223..4363)
                     /locus_tag="IV250_002713"
     CDS             complement(4223..4363)
                     /locus_tag="IV250_002713"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769942.1"
                     /translation="MSGKLLDVTQYELILKNNKIDSPIIIPKHAVKYVYGVAESNGED
                     ND"
BASE COUNT         1235 a          989 c          618 g         1534 t
ORIGIN      
        1 aaaaaaacaa cgacaacgtt atcacctgtt aaattcacca accaattttt cttaccaaaa
       61 tgcttttgtt tttctgccat tcttattcct cctccgttac atctaaccct tgaagccaag
      121 tgtctaattc ttttttcaaa acatctgctg attccgaaaa tgtttctcgt aaatgattcg
      181 tcaactcatc attttgactt tctcttttca aaatatctct caatttgtat ttcattctac
      241 gctctaaaaa cccccacatt tcagggttag caatcagtcc aaaaatcatc accttatcat
      301 ttgtttttac atcaagtggt agaaaatgag ggctagcaaa actatttaaa ctttcatgaa
      361 ccattgcatg ccaatctgtt gctttcccac gccttaactg caattccagt cgccaccaag
      421 tttctatttc tggtggcaca attttacgct tggtttcctg ttccaatttt ttgttgtata
      481 gccgaatctg gcgttcgctg gcacgactgc cccaataagc tgtttccagc ttcccagacc
      541 gcccataaat tggcttgaaa gaaacaggat caaccactcg atattgtgta ataaactcat
      601 caggcacatc gataatatcg caagcaatat ccgcacgtga gaaatgtggt tctagaaata
      661 aatcatgaat gaaatttttc attgaactag ctaaaaaatg accaatctta ttcgggttaa
      721 aatcaatccg ccctttgcca tcttgccatt tcaaaatctc aatataagca atattttctt
      781 cgtattggtc taataattgc cagccgttat tcccaaccgc ttttaaatag ccattgcctt
      841 cattcacccg cattaattgc tcaaaatcta aaatcaacac atcattttga gtgtgataat
      901 aaatattttc cttcagtttt ccaacgatag taattcggtc aattgaccac tgcattttta
      961 agggggtgta catattttgt acatttaacc gcctgttaga tagggctgtt aatttttccc
     1021 actcatttac gattatgcca tttttactca aattttgttc ctcctcaaag aaaaagagac
     1081 ttctggaaag aattttcaaa agttcacgta actcttaaaa attcattacc aaaaaatctc
     1141 tagtcttgaa gaaatctaaa aatattcgta taatgattaa gaagttacgg agctagtgcg
     1201 attagtttcc gtaaaggtcg agaacgttgg tagcgttgct cgactttatt tttttgctct
     1261 tttttcagtt tatttatttg ataactcaat aatagcactt ctctttagct atgcaaatat
     1321 ccaaaaacat aaaaccgcac cctttgaaaa gggcgccccc aaacttcgct aacgctaggt
     1381 tggccaaaaa atggactggc cattttttag caaatgtcag cttaaaaggc tagtggttta
     1441 gccgtcaact ccttatcctc ggcacaaacc gcctccggta ccgttgactg cacatcgtaa
     1501 ctaccactcg atctcatctt cagaacgctc taatttatgc tgtgagacgt tttgacgata
     1561 aaataaggtt ttctcctgct ctaatacttc taattcctca gaaagcccta tatccttttc
     1621 tgcaagacca aaacggatag acaaacgagt gatcgatttt tgaaatgcgt tttttaatcg
     1681 ctctaaacgc tggcgtaaag agcgattttc ttctttcagt tttttatttt ccacttgtaa
     1741 atctgctaat tgttttctac cagacaaatg acgctcttct tccaatgtag ctacatactc
     1801 atttaattct gtattttgct caagcatctg taaatattgc tcactcatgt tatcagcatt
     1861 tctcttggca tttgttgctc tcgtagctcg ttgatacaaa tcaacaaaat cattcccaga
     1921 taaatgatac gaaccatcag gaagcctctt gaaactttct aaaggaacca tatctttttg
     1981 gaaaatgacc gccatctctt tttttacatt cagcatattt ttcttctctt cttcaatctt
     2041 cgctctctca gaagccatca gagccttatc acgctcgttt tcttctcttt gcctataaat
     2101 atacctcaac tcatcagaaa cgctctcacg ctcttttaca gccgtttggc gcaattcaga
     2161 aatttcactc atgatttctt tgtattcgtg aatatcttta attttgttgg taccgacaat
     2221 atcccttttc caaccgtaat cattcattaa tcgttctata gagtcaactt cttgatttct
     2281 aaaattattg aaaagcactc gaccattatc tttatcagcg tgtatccctt gttgaaccag
     2341 tgctttatta aagctcggct gcttttgaac accccgttta taaccttcag caaccggcac
     2401 aatattcaag tgcaggtgcg gacttatttc gtcattatga ataacagcat tataaacagc
     2461 caaattaggg tttctctctt gaaactcctt gtaatactga accaaaattt tattggcaat
     2521 cactcgattt tgttcagctt gttgtttcga tatgccagta gaagaaccat ctgctcgatt
     2581 tgtctggaaa tcatctaaaa tgcctatttg cacaataaat tctcgttgag gttccaactt
     2641 tttatccttg cgcactttcg ctaaatagtc ctcgattttt ctatcggctc ttttttgttt
     2701 cgcattatat ttttctacca cttcgccaaa caattcctca tatttatctg ctagattcgt
     2761 ctgctctaaa taaatatttt catccgattt cgtgaagtca atatgcttat gccaatcatt
     2821 ggttttctgg ctatcagtta actcacgatt attatgagaa atacttgtat tcttcgttcc
     2881 ttttttaaat gaaatactaa accccaagtt aaaaccctcc ttcgcgctgt cgctaataaa
     2941 aagagtgtaa acctttctgt tactctttgt caaagagtaa cattttccta attgtgacat
     3001 ggctgtcaca attccaaatg ctctccgagg gtattcgctg tcacaagtga caacgaaaaa
     3061 aggagccgaa agcgtgtcac gctctcaact ccttttacgc tttattttgc ttcacgcaaa
     3121 ataatcgctt atcgtaaatt cacgtcatta aatattgacg taaatttact ctcactcatc
     3181 tgacttttct tattcggtat cactccgaat aagccgtcag attcatcggg ataatctcgt
     3241 tcgcttggcg ctgatttttc atcagcaagc cactcattat ccactgattt ttcgtgttgc
     3301 tgatcgtcag aaatcgacga tagccaccgc tcaaaatcag caaaagtttc aaacccaaaa
     3361 cgcttgcgaa gagccttgcc cattgcagca ttttctttct caatcaagcg ttttttctgt
     3421 tggcgaagtt tcgcttgttc ttttttaatt tttcttcttg cgctttaata tctgctaaag
     3481 tcattcccat aaactacatc tccttttctt tggaattaat aacggaaatt tggtttaact
     3541 gcataacatg actcattaat ttcaaccaaa attctttttc tgattcatca gttgtttgcg
     3601 ctaaatgctt atgaacaaat tcattagtca aaactgaaga ttttgctaga tcagcattga
     3661 taaccgagtt gagaacatcc atcattcgtt caagcatttc actataatta tcttcatccg
     3721 gcaaattcaa ataatcagct aaccgttcat acatcaattc aattctatcg actgtttctt
     3781 ctctcaaatc aattttatcc atgtcacacc tcacttccag aattgccacc attttcgatt
     3841 ttttaattct tgattttcta gtaacaactg atcgtttttt tgttgctgaa gcaaagtcaa
     3901 tttttgttgg tcacgaatca tttccgttaa ttcagaaata tgcctatctt tttccgctaa
     3961 ttgttcacgc aaaaattcat tttctttttc taacgggaaa tcattgcctg catcattgac
     4021 cggtaaatca ccagtcaatt gaccaccttt tgaccgataa attgaccgca caacaccaat
     4081 caattccttg tctaacatga taaccccatt atctttatac cgagcaaggt cataacctgc
     4141 attgaccagt tgattgcgca ctgtattttt agatataccc aactcatcag caaggttttt
     4201 tatggtcatt ttttctggca aatcagtcat tatcttctcc gttactttca gctaccccat
     4261 aaacatattt aacagcatgc ttagggataa tgattgggga atcaatttta ttatttttca
     4321 aaatcaactc atactgcgtt acatcaagta acttaccgga catttttttg ctatcc
//
LOCUS       DACDIE010000120         4340 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.123, whole genome shotgun sequence.
ACCESSION   DACDIE010000120 DACDIE010000000
VERSION     DACDIE010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4340
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.123"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..1197)
                     /locus_tag="IV250_002714"
     CDS             complement(<1..1197)
                     /locus_tag="IV250_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein"
                     /protein_id="HAP8769943.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLIS"
     gene            1455..1691
                     /locus_tag="IV250_002715"
     CDS             1455..1691
                     /locus_tag="IV250_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAP8769944.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            1710..1925
                     /locus_tag="IV250_002716"
     CDS             1710..1925
                     /locus_tag="IV250_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAP8769945.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(2628..3380)
                     /locus_tag="IV250_002717"
     CDS             complement(2628..3380)
                     /locus_tag="IV250_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8769946.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(3395..>4340)
                     /locus_tag="IV250_002718"
     CDS             complement(3395..>4340)
                     /locus_tag="IV250_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP8769947.1"
                     /translation="YVVFQIMLDSLFLLMFIWLYRKLAINRIVEQSSSKLTAIFMIYL
                     LTIGLFVSYVAHQYQVFDHFVFGVLIFLIIQMFVVLFLFIRITIRQREQYEQQLKRKE
                     LVYLKKYTDSLEKDQEKFARFRHDYKNLLLSLKEIANQNHDSILEKEIKELEIYSNSY
                     LTNKFEYRYLKNIKNEYLKSLLIAKLYQANNNKICCKFECPSTIIEVPVPIFDCIRVL
                     GIILDNAIEASIESQDRELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGITTKKG
                     HQGLGLSSIEEINKKNPNMFVNYQNDPYQFTTQVILLF"
BASE COUNT         1488 a          714 c          640 g         1498 t
ORIGIN      
        1 gctaattaat tgaatcaaat gtttaattcc ttgttgaata ttatcaagcg taaccgtacg
       61 aaatgatttt ttcattaatt taacaaattc atggtctata cggttatata ctttctcttc
      121 accgctatat gctttaattg tttctattcc tttaagactt tctatgatac tagaatttac
      181 agctgcaccg gctgccattt cttccctgtt cgatttttca tagcttttca cgaaagcgaa
      241 tattgcaact acataaaaag gcaaagagca tagagtgatt aaaaacaacg tactattctg
      301 aatagctaac gtaaccccaa caagcaacac cataccaata tctaaaaata ttgataacgt
      361 agcacttgct aaagcatcaa taatcttact ggcatcaaga aaacgagaaa taatttcccc
      421 tgattttctc gtagcaaaaa aattcattgg taaagttaac acatgtttga aatactgaag
      481 cataattgcc atactcattc gctgtcctaa aataataaga agataactac gactatattc
      541 aaacaatacc cgaaataaat agacagcgat caagccaatg gaaattatat ttaaagttga
      601 acgtgcttga ttagggatga aaaaatcaag aataccttga aaataatatg agcttcctat
      661 gccaaaaaac gtaatgaaaa tcgatgctaa aacgatgtgg aatactaatc ctttttgttt
      721 ccaaattata ggcaaaaaag aacttaatcc agctactttt tctttaatag gaatataagt
      781 ttctctaggc ataggtaata aaagaatacc tgtccattct ttttgaaacg cttcaatcgt
      841 ttttctattt tttccattag cgggatctgc aatccatagt acgtttccct tcgttttata
      901 aattacaacg tagtgcatat agctgttatc aaccaatata tgtgcaatta atggtaagat
      961 aagatctctt tctttccaga tctcactatc tgcttgaata gccatacaat caaaagacaa
     1021 tttttcaaat gtctttttca atccaaatgc tgaagtaccc tctaaatcag tcccagatag
     1081 gtcccgtaat ttatgaatcg ggatatcagt tttaaaatat tttaaaatca tcgaaaggca
     1141 agcaacacca caatcttttt catcttgttg ctgcacaaaa ggaaatgatt ttatcactta
     1201 tctaacacaa ctccttctaa aaaattttta tagaaaaatt ctcaatatag ctttaataca
     1261 gtgaaaatat tgtctcataa ttccacaaaa actttgaaaa cagttcttga acactaactt
     1321 tttcattcct gaatcaaatt tattttggaa attttactat tcaagaataa acttgataag
     1381 tttcaggaag atttttactg ttaatcaaat acgtatgtta cagtagcacc gtaaaaaata
     1441 ttatttaaag aagcgtgttt aaacatgcaa aatgtaaagg aactaagtat tgtggaaatg
     1501 cacaaactgt tggtggaggc aataatgctt ggggaaaatt ggggcaagtt gtaggtggac
     1561 ttaccacagg ggcagtaggt ggtgcaggtt taggaacagc aatttgcgga ccagcttgcg
     1621 gtgttgttgg agggttatat ggagctgtcg ctggaggtgc tgcagctggc tgggatgctc
     1681 gaaaaaaata aaaaaggaga ataagtagta tgcatattaa aaacacaaaa acaacattta
     1741 tattatcttc agaagagttg aaaaatattc aaggtgggag tgccgtggga gttttaggca
     1801 ctactttttc tggggcaaca gcaggagtaa aactttgttc agccggagga ccttatgcaa
     1861 tagctgcttg tggtgtaggt ggagctttat taggtgctgg attcagcatg tggaccggtg
     1921 cgtaaacaat taaaaaatat caaacctcaa gattggtttg tgtcgattac gaacataggt
     1981 atgttcttta tcttttcaaa agtttggaat ggacagaact ttttactaaa gcttcttgtg
     2041 ctagttttta taattatatt aaatttttat cttttattaa agtcagataa aaattctttg
     2101 gaaatgtaat ggtagagata tttttacaaa aggacttgca gcgctgtagt tggtgctgca
     2161 ggaggaccta tttcggccgt tacaggaggt attggtaatg tacttgtcgg atgtgcatta
     2221 aagcctgttc ctgcaagtta aatttataaa aacaggaaag taattatctc aaatcctatc
     2281 aagtacaatc ataggattgc acttgacagg acaaattttt taactaaaaa atgaaaaaca
     2341 ctttctaaaa attagtagaa tttgccaaat ctttcgcatc ttccaccata catttaattt
     2401 taattcccct ttatggtact taataaaaac aagaaaagaa ctattcattt ggcccttttt
     2461 tggttccaga ccatagaaat attcctccag taactaatat tgctgttatg cacgtaacta
     2521 ataacagcta taaaaattgg tacaacttta ataggattca gaaaaatttg cactagtgaa
     2581 ggaatctatt ttattagaaa aatgcaatct ccctaaagaa aaaaccacta aacagtgctt
     2641 tctttcaact tctccttaat tttttgtgct ttccctaaag taaagtttct ggccaactct
     2701 gaatcaaaat atagtgaacg ttttttaaaa tctatttctt taacgttaaa gatatttact
     2761 aaacacgctc gactaattct tgttaacata ggatatttct tttcaagctc actaattctt
     2821 ccataaaatt cgtactgccc atctttagta cacaatgata atctatgtgg taattttgaa
     2881 gcttctacaa aataaatgtc atttatatca aaagtaaatg tttgagaacc tatggaaaaa
     2941 acaaatgctt gattcgtaac ctgttttgtt gcatctatcc tttcttgcgc taataacaat
     3001 aactcaacaa tctcgtttcg atactcatct agtgtttgat cttttgttac aaatcctaac
     3061 gtttcaactc ttctttttat tgttacgggt aacatctcat catgagtagt tgtaaaaatg
     3121 atttttgctt gaacatcata ttttctgatt acttctgcta attctatacc gttaacttca
     3181 tgatttaaat caatatctaa gaaatatatt ccttgctttg gtcggaattg tttgaggtat
     3241 tttttaactt ctaacggact ttgtgttttc aatactatct taaatacttt atcgtgaaaa
     3301 agaatgaaat tatcaataat tcgttctaac tgatttaatt gtataatttg atcttcacat
     3361 aatattattg gataactcat aaatcctcct tccctcaaaa caacaaaata acttgagtag
     3421 taaattgata cggatcgttt tgatagttca caaacatatt gggattcttt ttattgattt
     3481 cctctatact agataaacct aacccttgat gtcccttttt tgttgttatt ccttttttca
     3541 ttagagtatc tatagagagt agtatctctc tacaactatt cgatatcgaa atttctatct
     3601 gtttatcatc tcggtaaata gctagagaca gttctctatc ttgactctct atagatgctt
     3661 caattgcatt atcgagaata attccaagca ccctaataca gtcaaatatt ggaacaggaa
     3721 cttcaataat agtacttgga cattcgaatt tacaacaaat cttattgtta tttgcttggt
     3781 atagcttcgc tatcaacaaa cttttcaaat attcattttt tatatttttt aaataacgat
     3841 attcaaactt attggttaga taagaattag aatagatctc taattctttt atttcttttt
     3901 ccaatatcga atcatggttt tgattagcga tttcttttaa actcagcaat aaatttttat
     3961 agtcatgtct aaatctggca aatttttctt gatctttttc cagtgaatca gtatattttt
     4021 ttagataaac caattctttt cttttcaatt gctgttcata ttgttctcgt tgcctaatag
     4081 ttattctaat aaataaaaat agaacaacaa acatttgaat gattaaaaat attaagacac
     4141 caaacacaaa atgatcaaat acttgatatt gatgagcaac ataagaaaca aataaaccaa
     4201 ttgtaagtaa ataaatcatg aatatagcag taagtttaga actggactgt tcaacaatcc
     4261 tatttatagc taattttcta tataaccaaa taaacatcag caaaaacaaa gaatcaagca
     4321 ttatctggaa tactacataa
//
LOCUS       DACDIE010000121         4301 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.124, whole genome shotgun sequence.
ACCESSION   DACDIE010000121 DACDIE010000000
VERSION     DACDIE010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4301
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.124"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..1015)
                     /locus_tag="IV250_002719"
     CDS             complement(<1..1015)
                     /locus_tag="IV250_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP8769948.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYI"
     gene            complement(1123..3150)
                     /locus_tag="IV250_002720"
     CDS             complement(1123..3150)
                     /locus_tag="IV250_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin"
                     /protein_id="HAP8769949.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            complement(3295..4026)
                     /locus_tag="IV250_002721"
     CDS             complement(3295..4026)
                     /locus_tag="IV250_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP8769950.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
BASE COUNT         1189 a          896 c          711 g         1505 t
ORIGIN      
        1 agatataaat ccaattattg tcttttttaa tcgtaacatc cgtcgtgttc aattccttgg
       61 ctacttttcc gtctattgta aaatcttttt caaataccca ttgattggta tagttgatcg
      121 tagtatttcc tttttttctc gaaaattttc cattttcatt ttcatttcct aaaacagtca
      181 ttgtaaggtg atattcacct ggggttagcg gttgaccatt taaagcagtt ggaaaagaaa
      241 aatttgtatt cggcgcaatc tgcaatcctt ctttttcctc ttgatataaa acttcagaat
      301 gtcctttttt tgtaattttt gtttcaataa ctacttgatt gatataggtt ttttgatcat
      361 tttgaacgtt tgctaagatg acatttcttg cattgatttg atctggttta acttcatgaa
      421 gagtaaggtt cggagcaact tcgttcagtg tctgttgcat caacaaagca ataatatagg
      481 tgtattcatt ctcaatcgat aagctttgat ccttagtttc tgtttggtcc ttttccgtct
      541 ggatttcttg gaatgtgatc ccaccagcca atacaccatc aaatcgctca ttcggcatcc
      601 tgacatgaaa agcaacggtt tggacacttt taggttttag cgtcactatc tgtggatagc
      661 tcacataatc taccagatca aagatcaggc tcatgtcttt ttcaatctcg ttttttccgt
      721 attcaaccat cacatttgaa ttggtggttg cgctattcac agaaatatcg attttcactt
      781 ctttttctgt atcatttctc aattccgctt ttacttctac ttcctgatcc ggatcaagtt
      841 gaagatcgaa gtaagttttt ctcttgtcga tttgattctc tgaagttatc ggcgtcactg
      901 aaaaattaaa tgcactagct gacactgata tattgttaaa aagtgcccct aaaataacta
      961 ggagtgttat aaataattgt tgttttggtc tcaagaaaat cgctccttta atcatatgta
     1021 agagaagact ataacaaaat cttgtctctg tgggacacaa gagcctatgg tacctgtgcc
     1081 cttcttgaaa ctcaataatt ttgttatatt cttctcttcc tgttaatttc ctgggacatc
     1141 tgcaagtatc caagatagtt tcccctcaaa ggttccgatt ctttggtttt ctacaggaat
     1201 atttattctg attccacgat gagctgtaga atctaaaaga gaggacaaat cgctttcacc
     1261 cttttgatca ctttcgaaaa tcgtaacagc ggatgtccct ataaccgttg tattttcaga
     1321 ggtaacaaag gaaagagctt ctggaagctc aaaatctcca tttgtaagaa ctttctcttg
     1381 tttcaattgt aatttccaac ttccaaaagc ccgttcatct ataattttca acccacctga
     1441 aaactgtccg aattcactta atggtctact ggatatttta tgttttccaa aattgacata
     1501 ttgaggagca gttgagagat aaagtttgcc ttttctgtaa taaaaataga tatcctctgt
     1561 attttcggag aagatgcctt ctgtattcgc tgaatccgag tcaaaaacat acccttcaat
     1621 atcaatagga gtttctttat acttctctcc tattttccca gtgattgttt tcatttcagc
     1681 aatatcaatc tgactgtctt tatctttata ataaacatgg atatctatct ccttcagctt
     1741 tgcgatcttt aggtttattt cattcgattg ctcaatcaaa ttcccgtttt caaggtattc
     1801 aaaatgcata gtgagtactt tttcaggaat tacttcctgc aaagttgtct ccaattcgat
     1861 tattaacgta tctccagcaa taaaataatc tgtcagattt agcgtgactc gatttccggt
     1921 tatcaagata tctttggcat caatttgatt tccattgatc aagatatgct cgggaatagt
     1981 taattcttgt gggaggtcga tactagtctg cattttctta tattcaagaa aatccccctc
     2041 gaccttcaat gtatttctga tagttatctt atcgtttcct ccagcttcag cgatggcccc
     2101 ataatcacta tcgttgactt ttacatcagc tgttgatgtg acattcgatg gaagtcgcgt
     2161 gacagcgaca gcattttctt gaaaaaaagt acctgttgaa gaagtaaacc cccaatttac
     2221 ttctgtagaa ccaaactgcc taatgagatc aggtataatc cgagaagagc gaccgacacc
     2281 ttccaaatca taagtaagac ttctttgctc cgcgtcccat ttaattatta acaagcgcca
     2341 tgtgccatta gaaagaaatg taggagctac cgtcacgcca gtatgctctc cagaataatt
     2401 attattattt ggtttaggcg tgacaaaagc gacatgtcca tgccctctat tatcagcagt
     2461 tatctctcga tccattcgat cagaagaacc actattttta tacgtatcaa actcaatcga
     2521 gagcgcattc gcaatgtaag gttggccaga acgagtagaa tatgagccaa ttcccatacc
     2581 tggcgatcca ataacttgac tgggattact agccattcta ggatcgccag tcaatgtaaa
     2641 ggtcatgcca tctcccgctc tatctcgttg atttcctaag tataaataag atttaaatgt
     2701 aaaatccgag agtaaactaa tcttcttatt tgaccaaata gctcctcttt gagacctttc
     2761 tgctgggttc aactgcagta acttcccatt ttccaatatg gacgtgctag taccgatagg
     2821 tgtttgaaaa attttatcga tagaaataat attgggatcg tcaggcagct caaccgcaga
     2881 aattggtgca tcttctagtc cttgtttgtc cgaaccctca gcttctacgt tgtcagtggt
     2941 ctctccacta ctcgaaatac ttaaatcctc actgctttct tgtgtctggt tggattctat
     3001 taaactgtcg aatgtcgaag tgagtttgct ttcttcgtct cttctatttc catgctccaa
     3061 ctgattcaat tccaaagagt accctatgat cggaaagata ttagtcacta agagactgct
     3121 taaaatgagc acgatcctcc tctttttcaa tatatctttt cctcctgatc gttaaattcc
     3181 tagatgaact attcgtatcc aaacaaattt agcctagcct ttgacaattt ttgctcgatt
     3241 acctcgcaag acaatatgac tacgtattta tttttaacag ttcttttcag taaactagtt
     3301 gttaggtgta tcttctaatg tccaaactaa tttagtggaa tattgttgcg ctaacttgac
     3361 agatttaccg ggtaccgaaa gctgtaccgc atcttgattt tcattttcat ttgcaccaaa
     3421 tcgatagacc caagtcccca cacctgttcc tgatttagca ttaattgcta acgtattgtt
     3481 tcctcccggt gtcaacgtta attcagaatt caccgtttgc ggcgtatatg ttggatcaac
     3541 caaagaaaca gcctgccctt tcgtaaattt cagctgtgct ccaactagtt catccccact
     3601 ggcagtttta aactgttctg gttgagagac acttaatttc cacccagata aggtacttct
     3661 cctatctgtc acttggacat atgtcggttt tctagaccca tcgttcatca catcagcagc
     3721 tgcataatac gttttatccg cagtagaaat tttttgagtc ccaaatttga attttgatcc
     3781 ataatcaatc gataatgccc ctcctgttcc agggcttgga ggatctgatg gatcgacagg
     3841 tttattggga tcaggattct ctggatcaac aggaggagtt acaccctcat ctcctgcttc
     3901 aaatgtaatc gttccttctg tgttatattc ctttgggtat acttcagttg cagaaacaaa
     3961 agtcgtactt gctaccatgc tgaataacat cattgctgct gttaatacga tttctttttt
     4021 catcattgac attcctcttt ctttatttac taaacatatc agtcgattgg attaatccat
     4081 agcaaaattc tgttttacga tacttatgaa ataaaagaac atagacctat atttggtcct
     4141 atgtatcgat tagttataaa tgattatcag cccgatttga tttctagttt ttattgcaac
     4201 ttgatgcaaa cacaatctag aaatacagct gtcatagcct ttaaaataat tcgtaagtcc
     4261 ccctcctatc tatcaaaatg tgacttgacg agagtgtaaa a
//
LOCUS       DACDIE010000122         4238 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.125, whole genome shotgun sequence.
ACCESSION   DACDIE010000122 DACDIE010000000
VERSION     DACDIE010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4238
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.125"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            75..503
                     /locus_tag="IV250_002722"
     CDS             75..503
                     /locus_tag="IV250_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP8769951.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGKYLGVKYEAK"
     gene            490..732
                     /locus_tag="IV250_002723"
     CDS             490..732
                     /locus_tag="IV250_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8769952.1"
                     /translation="MKQNKRYIPFIPLTTIKAAASGDSIAMSIVLSKYQNYISRLAMK
                     PVYEEEKGMVMYVDEYMRRQLETKLIEKILEFDTTR"
     gene            complement(1032..1247)
                     /locus_tag="IV250_002724"
     CDS             complement(1032..1247)
                     /locus_tag="IV250_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769953.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWK"
     gene            1354..1668
                     /locus_tag="IV250_002725"
     CDS             1354..1668
                     /locus_tag="IV250_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8769954.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            1772..2950
                     /locus_tag="IV250_002726"
     CDS             1772..2950
                     /locus_tag="IV250_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8769955.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            3353..3646
                     /locus_tag="IV250_002727"
     CDS             3353..3646
                     /locus_tag="IV250_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HAP8769956.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
BASE COUNT         1449 a          635 c          821 g         1333 t
ORIGIN      
        1 tcctagattg tagtaataat ggaaagggat agaagtaaaa cgagttgaat gaattttttg
       61 agggggagaa tattatgggc gactcagagg aaacccacat tcaacatacg tttgacagtt
      121 tttgtaagaa agtagtctgc aatgaggcgt taaatattca aaagaagtac gctagatttc
      181 gccaacggca aatatcaatg gaaatattgg aacaaaaagg gttaggtaca gagttctatt
      241 atttggaacc aaatttgaat agtagtggta cttttattgt ccttggtatg aaaatcttta
      301 tttccaatga ggagttggca aatgcgattg cagcattacc tagtcaaaga caagaaatca
      361 tttttatgtc cttcttcatc ggtcttaatg atcgtgaaat tggcgatatt attgggaaaa
      421 gtctcggcag tgtttggtac caacgacaaa tggctttgga agagctagga aagtatttgg
      481 gtgtcaaata tgaagcaaaa taaacgatat ataccattca taccactaac gacaatcaaa
      541 gccgcagctt ctggtgattc aattgccatg tccatcgttc tgtcaaaata tcaaaattat
      601 atttcacggt tagccatgaa accagtttat gaagaagaaa aaggaatggt tatgtatgta
      661 gatgaatata tgcgtagaca actggaaaca aaattgattg agaagatact tgaatttgat
      721 acaactcgtt aaagtatttt ttccctttcc ttttcattct atggaagttt tttatcgggt
      781 caaaataaag gatttccata ggctgaaaat agcctagcgc tctttgacaa atgaatagca
      841 aatctatcag tacatgtaga ttttgattct ctagttacgc catgacaagg ttagctttta
      901 agcgacaaat tttaatatga gaatcgcgac tacgataatg atacttccgt aaccaaatcg
      961 gcccgccatt actgggggga ggtgagattc ctatgtgctt tggcaaaaca gctgatagat
     1021 cagaaaaact actatttcca tatttcgatt ttggttggca tatttttcat gccaaattct
     1081 gtgattttaa tagcatcggc aagatttaat ttttcttcag ctctctctcc acttttaagt
     1141 ttttttactg tactcaagtt gattcctgtt ccgaatgcta ttgcattagc cgtagatttt
     1201 tcaagaattt ctttaattat ttttatgtct gcaattcctt gtttcatatt aaaaattcct
     1261 cttttctagt ccgttttgat ggagtactgg agtgtttttt tacaatttta aggggtaccg
     1321 tggtggtcgt tttgattttg agagagaagg gtgatggagt tggaagttaa aacaaatgtt
     1381 aattctctgg aaatagagtt ctattcaatt ttacaaaaaa aactggaaga ttacacggaa
     1441 aaattgcttg cagtcagtca tttacctctg gtattaacaa atactgattt aaagcgacta
     1501 tttcagataa gtgatagtac actgaaccgt ttagtaaaac ttggtgagtt tcctccctgt
     1561 tggtacggga ttcgaggaca ctattttcgt gatgatgttt tagaatggat gagaaagagt
     1621 gattcgaatg actatcgaga aaaattgcga ctacttcgat ctttataagt tacattttct
     1681 ttctaagatg cggtacaatg atgacataga tagaaagatt tccaaagggt ttctgcttgt
     1741 tgggttcctt caaaatagga ggaatcaata aatggaaaaa acagatcgat tcaagaagca
     1801 aatcagttct actggtagga tcacatggta ctttcaagca tatcgaacaa caagacgtgg
     1861 ctttaattca aaacgtgagg cgcaactcgc ttatctggaa atggagaaac aacaaaggct
     1921 taagaaaaaa attgttgcaa gcaatgacaa gtttagtgtt gtaggtgagc aatggttcca
     1981 atattaccgt tcgttaaatg aacaaaagga aataacctat gataagcgag aggaacaatt
     2041 aaaaattatt gttcgatgga ttggtgataa aaagttgtct gatttgacac cagagtgcct
     2101 tcaagaattg ctttttacct taaaggaatg tggcgttaat ggaacaaatg ttggctatgc
     2161 caaaaatagt ttacaatcac ttattcaagt tctaaatatg atttttaaat attgtcaaag
     2221 aaagaatttg ataagtgaaa atccaatgaa gtctgtgaga atgccaaaat atcagttaac
     2281 ggtaactgat ttaaaagaag ccataaataa tattgaggac aagtttctta ctgtcgatga
     2341 attgagaact tttttgaatt atggcattgt tcatgaggaa ttaccgatgt ctgtactttt
     2401 ccatgttcta ttttacactg gctgccgagt cagcgaggcg cttgctctac agcctggaga
     2461 tatagatttc aaaagaaatg aaattctgtt ctataagcaa acggcagtta aaggtaagag
     2521 taaggatttt cgaattgaaa ctactaagac agttagttct gctagacgag ttctagtaac
     2581 tcctctagtt atggaaaagc tacaacagtt gattaaggct ttgggtgaga tgaggcaaca
     2641 ttacaggttt attatagaag aaacatattt gtttgtgtat cttgattcta gtaaacgtgg
     2701 tgttccttat cgtcgagagt atgtaaatga tcacgtcaaa cggtgcgtga atcgctcagg
     2761 aattaataag ccatttcata ctcatttagc tcgtcataca atggcaagct tagtagcgga
     2821 acactgtagc tgggaagtct tgaaagatcg cttaggccat actgaccaaa caacttcgaa
     2881 aatttatcgc cacttaacgt caaatgaaaa ggtaaaacct ctgattgctt ttagttcttt
     2941 agagagttaa aaaaacggtg aaagcactgg tattacagga ttcttgaaaa acgatttttt
     3001 aacaaattag tcaatatgta gtcagaggta ataaaaatcc ttttatatca atactttaac
     3061 gacttttcac aagtatcaaa gaaatccgtc gttaatcatt gttatcagac tgagaaagaa
     3121 cccttggtat tcaagggttc ttttttctta gcatgtaatt tgaacaccct gatccagttt
     3181 tttctttaat tattggtagg ggagaataat ggattagaat aaatctacct ataagtagta
     3241 gtaaaaaaaa gagcactatg ataatatctt tcttaagagt aaccgcttta ataaaaaatc
     3301 cagaatattc taacataaat ggagtattct tagaatcaaa aggagaaaga agatggttta
     3361 tacttatacg gagcaagaaa agatgaaagt tttagacaca ttcatgaatg atggaaaagt
     3421 aaaacaagta ccaagttcaa caaaaaagaa atacatccta ttgaaagaag tcttgaaacg
     3481 ctttgaccat ggagtgactt atacagaaac agaggttaat tcgatactgc tgaacgtatt
     3541 ttctagtggc gattatgtcg aacaaagacg ttatctaatc acatttggct ttttcaaaag
     3601 aagttcagac ggtcgggcat atcagatgat gggaattgaa aattagaagt tttaatttat
     3661 actcattgaa aaaagttcaa gtatgcgata ttggttctac accgcatact tgaacttttt
     3721 ttgtcatctg tatctataat acaaagccaa gaacgttatt caaaatatgg atagctatga
     3781 taaaagctaa tgagcgattc gcagttaaat aagcaatcgt cagtaaaatc gttccaggag
     3841 cgtacatgaa aaaatcaatt ggatgcgtta catgaataaa gataattctc ttttatttgt
     3901 acgaataaat accaaaacgt acctcctaaa attagatttt ggacagatgc cctttaaaaa
     3961 gataacaaga tttcgttctt gaaacaatat atatgctttt attccaatga ggttgtgaaa
     4021 acagtatttt gtcctgagcg ttagaaaatt gaaaatgggg aatagcaact cgtactttac
     4081 cattttttaa gtgaatacaa gatcagctag ttgaacaccg tttattcgtg atgagggaca
     4141 caacactgca gtcgacacat ttctctttta tgttcaaaga taacaaatat ttaaaacaaa
     4201 gaaaaatagt agataatccc gtcctaactc ttttattc
//
LOCUS       DACDIE010000123         4157 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.126, whole genome shotgun sequence.
ACCESSION   DACDIE010000123 DACDIE010000000
VERSION     DACDIE010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4157
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.126"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            179..775
                     /locus_tag="IV250_002728"
     CDS             179..775
                     /locus_tag="IV250_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP8769957.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            899..2698
                     /locus_tag="IV250_002729"
     CDS             899..2698
                     /locus_tag="IV250_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP8769958.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2976..3188)
                     /locus_tag="IV250_002730"
     CDS             complement(2976..3188)
                     /locus_tag="IV250_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769959.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(4052..>4157)
                     /locus_tag="IV250_002731"
     CDS             complement(4052..>4157)
                     /locus_tag="IV250_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8769960.1"
                     /translation="ASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
BASE COUNT         1228 a          707 c          729 g         1493 t
ORIGIN      
        1 tttaaaaaaa acaagttatt aaataagtct ttagttggat gtttttttga gatcaatctt
       61 ttatacttat gatgaactta caaaattgtt tgttttctgt catatcaaaa aatggcgcag
      121 ttagttgctt ttctagtaaa gtatagttaa caaagaacct ctaaggagtt gatgaataat
      181 gaatattgct gcctctcgaa aactgataaa tttcatatcc attatcggat taggcctatc
      241 aatcgcgctg acgatttatt ttattaattt aggtgttttc aaagatttga atgcgttaag
      301 aggattagta ggtgattcca tcattttagg accgattatt tttgtcttca tccaaatcct
      361 gcaagtagtt attccaatca ttcctggagg gatcagtact gcagctggtg tcctgatatt
      421 tggcccttat gctggattta tctataacta tattggaatc tgcattggtt cgattatcat
      481 ttttctgcta gggcgtcgtt atggaaagcc attcatactt tccatgatta gcgataagac
      541 atataacaaa tatattggct ggctggataa tcaaaaccgt tttgagaagc tgtttgctct
      601 agctattttt cttccagttg ctcctgacga tgcgttatgt ttgatggctg gattgacaaa
      661 catgtcagtg aaacgatata cactaatcat tttgattgct aaaccactgt cgattttctt
      721 atatagtatg gccttgattt atggaggtca atatctaagt ggtcttcttg gataatattt
      781 attttgtaaa attagtcatt gattgacaat gttaaatctg ggaaagggtc tgcaaaacac
      841 actgcaccct ttcctttttc attagatttg cgctacaatg tgaagagcag gtgataggat
      901 gaattatttg aaacgtagct taggtaatgc ttgggacttt ttaaaaggaa cacaagctta
      961 tttccgtgat gtcttgctga tgcatggctt tattttattt atttgtttac ccttattgag
     1021 cagtacgacc cgttttattt tacaccgagg ggcaattgac tatttgtctt atgacaatat
     1081 tccttcaatt ttttctcaac atcctggggt tctactctcg ttaatcgtag ttttgatgac
     1141 gattttactg ttagtttatt tcgaatttac ttttcttttg atgagtgttt tttttattaa
     1201 aaagaaagaa ccaatctctt tgaagcaact cttacacttg acgatacttc agctgaagaa
     1261 agtgagaccc attacttttt tgtttttcct tgcttatttt ttattgattc taccgatcag
     1321 cggattaagt tttaattctg atctactgtc aaaaataaaa atccccgctt tcattatgga
     1381 ttttattttt gctaatcgtt ggatcatcgt cagttccttt ttactcgttt atattttcct
     1441 tggctatatt gggattcgac tgatttttgc tttaccggaa atgattctac gggatcgtcc
     1501 ttttcgagca gccattcggg aaagctggtc attgaccaaa tcgcgtcttt tggccatcac
     1561 gggccaattt attgtgattg gcggtacgat tttactcctt tcttctttag gatatatcgt
     1621 tgttattttg gcacaaagta tggttgaaca atttttccca gactatgcat tgatcagcgc
     1681 tgtgtttgct atgacgcttt tacaagggat tttattattc aatatcgtga tgtctaccgt
     1741 agggattttt tatatcattg ttgatttcat ggatgacgaa ggcttcttgc ctgagatccc
     1801 taagtggttt atcccacaag cccccaactt gcgtttctct gcgctgaaaa acactggttt
     1861 gactcttttt gcagtttttt tcggtattgg cgtctgtctt tacaatatgg attatctgac
     1921 ttcagctgtt cagacaaaac cagtgactgt ctctcacaga ggtgtgagta cccaaaatgg
     1981 ggcgcaaaat actttagaag cacttgaaaa aacaagcaga gactatcatc ctgattatgt
     2041 agagatggat gttcaggaaa caaaagatgg tcatttcgta gtgatgcatg atgcaaatct
     2101 tcgtcactta acaggggtaa atggaacacc acaggatctt acattgaaag aattgacaaa
     2161 tcttacagtt actgaaaacg gagtgcaagc ccctgtttgt tcttttgatt cttatttgga
     2221 aaaagcacat aagctaaacc aaaaattact tattgagatc aagacctcta aaaaagattc
     2281 ggatgacctt gtcgaacgat ttgtccataa ataccgcaaa gttatcttag agaaccatca
     2341 tattttgcaa agtttgacgt accaaaccgt ttcagattta aaagaagcag aaccaaactt
     2401 ttatgtgggc tacatcttac ctttcaatat tgttgggccc cctgtgacgc ctgctgattt
     2461 tctgacaatg gaatatagta cgatcaaccg taacttcatt aattctgccc accaagacgg
     2521 aaaagaagta tatgtatgga caatcaacga ttcagatgct atgagtcgca tgatgtttta
     2581 tggtgtggat ggtatcataa ccgatgagat gggactacta aatgaaagtg tgaaacaaac
     2641 agatgaagaa atcacttatt cagataaact gctaaatttt gttttgggag tcggctaatc
     2701 aaaaatcgtt aatgaaaaaa gtctttatac aacaaaaaac gcataacctg ccaccgcagg
     2761 tatatgcgtt ttttgtttga cgctcataat gtgtgcttaa ctttctcttg attccttcat
     2821 cgcacgaaca atagctaaaa tagcaggaat tgcagagatc actgcaagaa taattcccat
     2881 tttccctgaa ctttttcttt ttggcatatc ttctcgctcc cttctgtctt tatttatatt
     2941 ataagggagt tcttgcgaat tctcaaaaat actgtttaag aaaccaattc agcaggtgcg
     3001 ctaatatcac agaattttac gagcatgcga tacttgcttt cattgataag ttgatagtct
     3061 cgttggtcat aactttcaac atacacgaca gccgtgttga tatatgtacg ttcaatacag
     3121 ccgattagtt catgagatac tccgatcggt ttgcatatat aagatcttcc tacttctgct
     3181 tttaccattt cattccctct tttctttatt tttattttgt tttttcttta ttttataata
     3241 acaaattacc ttgtatttga aaacgttttt atccattttt ttatttttta ttttgagtat
     3301 ctttctgctt tttagataac tattttttaa cacgatctta gacgttgtga caaaaacgtt
     3361 tgtttatatt cctatgttgt tcgtgcgttt ttttctttat attgctaagt tatttataaa
     3421 gaggtgagca tatgatttat attcaagcaa tagtatttgt aaccttgatg attgggctac
     3481 ttatcggatt aaaagaaaat agctaaacca tttaagaaaa aataaataag gaaaatgata
     3541 aatgcttttt aattgcatca aaaaacgtac gatgctaaac ttggtataag ttcgaaagtt
     3601 taaaggaaac tttcaagaac taaattacat tacaaaggag atgaatttta tgacgtttac
     3661 aaaaacaaat atgcaatacc gcgtagcttt tgatacaact tctaaccgtt ttatggcgat
     3721 tgatgcacaa aacgaaaata acgttgcgtt tggtattaca atcgaacaag cagtccgagc
     3781 attaaacgaa tctagataag gagtgaacca ttccaaatgg cgactgatga gatctcggaa
     3841 aatcaataaa agacgtttgt aacggtaatc cgcgacaaac gtctttttgt attcattaca
     3901 tatatttgga aattgtctct ctttatctct agacctaaac aaaaacacaa gttctaatat
     3961 caaccatctc gttttttatg acaattttgt tagctgtttc gatgataata ttccgttatt
     4021 atctgtataa tatttaatat aatcttaatt gtcaaattaa gttagacaga tcatcaatac
     4081 ttacgtaact caaaaatact tccaaaggtg ttcgataatc cagtgatttt ctaggaatat
     4141 tatttcgttt agatgcg
//
LOCUS       DACDIE010000124         4129 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.127, whole genome shotgun sequence.
ACCESSION   DACDIE010000124 DACDIE010000000
VERSION     DACDIE010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4129
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.127"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            36..1262
                     /locus_tag="IV250_002732"
     CDS             36..1262
                     /locus_tag="IV250_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769961.1"
                     /translation="MIFYMFININEVLKMTTITMTKNIEVYVDGSQEERDSFFDFFWK
                     NSREYVKALNLVYKQLMFEELIKDAIKEMDQGYAEREESIQNRIEKLAYTDPIDQKKL
                     DKAKAELSKLRNEKNKEAREILTQAIGLKSQTRTRDVAKKLDFDFGDFVDSANMKASK
                     DFGNDFIGIVTGQRTPRIYRNKSKYGLLPFRGRDIKIHKADKFYISLPKGFRLKVNLG
                     SKPRKARDVASVLQHIMDGDYKLNQSNLIFDGRKLFFNLTISFEKSYNEMEFKKGNEL
                     IVNFADLCSGECLINDKSVMSFGDSQYIRNFKSKILAMKSKEQSRGVFSKGGHGRKRK
                     LDQDRWEAIKKRESNFVKTYNNQCSAKIVKTAIRNKAQIIRIIEPDKAYGEWAYYQLK
                     EQLVNKGKRYGIEVRS"
     gene            2187..3512
                     /locus_tag="IV250_002733"
     CDS             2187..3512
                     /locus_tag="IV250_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP8769962.1"
                     /translation="MFDYSREPVNDYFLIDMKSFYASVECIERNLDPMTTPLIVMSRS
                     DNTGSGLTLASSPTAKKKYHITNVSRANDLPYPLPKELYIVPPRMNLYIKKNMEVNQI
                     FRKYVADEDLLIYSIDESILKVTKSLNLFTSSETRSERRRELAKIIKDEIEKETGLIA
                     TVGIGDNPLLAKLALDNEAKSSKEFIAEWTYETVPEKIWKINEMTDFWGIGSRMKKRL
                     NHMGIMSIEDLANWNPYTIKSQLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPKDYTRREEVELVVKEMAEQVAIRIRQHNCKTGCVRLAIGTSILEEKSGFSHQMKI
                     PITDNTKELQNYCLFLFNKFYEGQEVRHIGVTYSRLVYTDSLQLDLFSDPRKKIAEEN
                     LDRIIDKIRQKYGFTAIIHASSMLEGARSITRSTLIGGHAGGLGGLHND"
     gene            3505..3852
                     /locus_tag="IV250_002734"
     CDS             3505..3852
                     /locus_tag="IV250_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769963.1"
                     /translation="MIEYNDRGRLKWSGFYLSDHTERINADNVQRANHNLAKEQMTTE
                     EIAKILRTAVIKNRPVCIQKEERNAEGYYPDDVVGKIIGYDELGVFVEEEKVHYDEIR
                     NVSFAELLKWSQI"
BASE COUNT         1606 a          529 c          792 g         1202 t
ORIGIN      
        1 tcatgtgaat atgagcttaa caattaaacg tatttatgat cttttatatg tttataaaca
       61 ttaatgaggt gctaaaaatg acaacaataa ctatgacaaa aaatatagaa gtatatgttg
      121 atggatcaca agaagaaagg gattcctttt ttgatttttt ttggaaaaat agtagagaat
      181 atgtaaaagc gttgaatctg gtatataaac agctaatgtt tgaagagttg ataaaagatg
      241 caattaaaga aatggatcaa ggttatgctg agagagaaga aagtattcaa aatcggattg
      301 aaaagttagc atatactgat ccaatagatc aaaaaaaact tgataaagca aaagctgaat
      361 tatctaaatt gagaaatgaa aaaaataaag aagcgagaga aatattaacg caagcgatag
      421 ggttgaagag tcaaacgaga acacgagatg tagcaaaaaa attagacttt gactttggag
      481 attttgttga ttcagcaaat atgaaagcta gtaaggattt tggcaatgat tttattggaa
      541 ttgttactgg tcaaagaaca cctagaatct atagaaataa aagtaaatac ggattgttac
      601 cgttcagagg tcgggatatt aagattcaca aagctgacaa gttttatatc tctttaccta
      661 aaggatttag acttaaagta aatttaggta gcaagccaag aaaagcaaga gatgttgctt
      721 cagttttaca acacattatg gatggcgatt acaaattaaa tcaaagtaat cttatttttg
      781 atggaagaaa attatttttt aatctaacaa tttcttttga aaaaagctat aatgaaatgg
      841 aattcaagaa aggtaatgaa ttgatcgtaa actttgcaga tttgtgtagc ggtgaatgtt
      901 taattaatga taaatctgta atgtcttttg gtgatagcca atatataaga aacttcaaaa
      961 gtaaaatttt agcaatgaaa tcaaaagaac aaagccgagg agtcttttct aagggcggtc
     1021 atggtcgaaa aagaaaatta gatcaagatc gatgggaagc aataaagaaa agagaaagta
     1081 actttgttaa aacttataat aatcaatgtt ctgcaaaaat cgttaaaact gctattcgca
     1141 ataaagctca aattattcgt atcattgaac cagataaggc ttatggcgaa tgggcttact
     1201 atcaattgaa agaacaacta gtaaacaaag gaaaaagata tgggatagaa gtcagaagtt
     1261 agtaatttaa acaatcgttg atttggcaac gaaatagtga gggaaaatcc actcactgaa
     1321 gggtaaactc cgtcaagttg tgtgaccaga cggaaggacg aatgttgttg aggttttaga
     1381 actatataaa acagcatgtt tctcaaatta tgagatgtta agaaaaatga ttcgagaggt
     1441 tttagaacta tataaaacag catgtttctc aaacaaagtg cagaacgaaa atcaattcat
     1501 cacagtttta gaactatata aaacagcatg gttttaaaac aagaaataag tcagttttcc
     1561 ttttgcttta gtaaggaaaa atagagtatt ctcaataaaa cttaaaataa gaaagaataa
     1621 gcaaaccttt gttttaatag ctaatcgaag taaaacccaa agccatatga actcctgaat
     1681 cagcgaaagt ttatgatgat tgggcttact aataatttag gaattagatg gtaaatctaa
     1741 attaaacaat cgttgatttg gcaacgaaat agtgagggaa aatccactca ctgaagggta
     1801 aactccgtca agttgtgtga ccagacggaa ggacgaatgt tgttgaggtt tagaaccaca
     1861 taaaacagca tgtttctcaa attgccttag tggttgaatt gagatctatc acgttttaga
     1921 accatataaa acaacatagt tctcaaattg tttaaaatga ttgtcaagtt aaaaaaatct
     1981 gggatcatat aaaatagtat agttctcaaa tatgattaga aaccctatta aagagcaata
     2041 ttaaaccaac ttacttaaat cgtttaatta agaacagtaa tctcttttag tcagactttt
     2101 agtctgactt ttttgtattt ccaaaattta ttctttacaa aagcgaacgt acgtttgtat
     2161 aattgcttgt gaatggggga ataaaaatgt ttgattacag tagagaaccg gtgaatgact
     2221 attttttaat cgacatgaag tctttctatg ctagcgtgga atgtattgaa cgaaatttag
     2281 acccaatgac tacaccgtta attgtcatga gtagatcgga taacactggt tcaggtctga
     2341 ctctagcttc ttcacctact gcaaagaaaa aatatcatat cactaatgtt agtagagcta
     2401 atgatttacc ttatccatta ccaaaagaac tttatattgt tccacctcga atgaacttat
     2461 atataaaaaa aaatatggaa gttaatcaga tttttagaaa gtatgtcgca gatgaagatt
     2521 tattgattta ttcgattgat gaatcaattt taaaagtgac aaaatcctta aatttattta
     2581 ctagttcaga aacaagaagc gagaggagaa gagaattagc taaaataatc aaagatgaga
     2641 ttgagaaaga aacaggacta atagcaacag tcggaatagg agacaaccca ttattagcta
     2701 aactcgcact ggacaatgaa gcgaagagta gtaaagaatt tatagctgag tggacttatg
     2761 agactgtacc tgaaaaaata tggaaaataa acgagatgac tgacttttgg ggcattggat
     2821 cacgcatgaa aaaaagattg aatcatatgg gaattatgag tatagaagat ttagcaaatt
     2881 ggaatcccta cacaattaaa agtcagttag gtgtaatcgg acttcagtta tatttccatg
     2941 ccaatggaat tgatcgtacg gatattgcta ttccaccaga accaaccaaa gaaaagtctt
     3001 atgggaattc acaagttcta ccaaaagatt atacgaggag agaagaagta gaactagtag
     3061 tcaaggagat ggcagagcaa gtagccattc gaattagaca acataattgc aaaactggtt
     3121 gtgtacgctt agcaattggt acgtcaattt tggaagaaaa atcaggtttc tcccaccaga
     3181 tgaaaatacc aattacagat aatacgaaag aattacaaaa ttattgtctt tttttattca
     3241 ataagtttta tgaggggcag gaagtaagac atataggagt aacttatagc agattagtat
     3301 acacagattc cctacaatta gatttattta gtgatccaag aaaaaagatt gctgaagaaa
     3361 atttggatag gattattgat aagattagac aaaaatatgg atttacagct attatacatg
     3421 cttcatctat gttagaaggt gcaagaagta ttacgagaag cactttaata ggaggacacg
     3481 caggaggatt aggaggtttg cataatgatt gaatataacg atagaggacg tttaaaatgg
     3541 tcgggatttt atttgtctga tcatactgaa agaatcaatg cagacaatgt acaacgtgct
     3601 aatcataatt tggcaaaaga acaaatgacg actgaagaga ttgcgaaaat actgagaact
     3661 gctgtcataa aaaatagacc cgtctgcatt caaaaagagg aacgaaacgc tgaaggttac
     3721 tatccagatg atgtagtagg aaaaattatt ggatatgacg agcttggtgt atttgtggaa
     3781 gaagaaaaag ttcactatga tgaaattagg aatgtttctt tcgcggaatt attaaaatgg
     3841 agtcagatat gaacattatt aaaaaatacg aattcggtta tattacgtat gaacagctaa
     3901 tagaggaatt atggggatat ggtcaacaat taattaatga agtggggatt gattgctttt
     3961 gtttctatat tgaatccgga gcaggatacc ataagaacag atactatata tttccatatc
     4021 caccaaaata gtctcttaga gatttttaaa ttttctccaa atactttcaa ttattgtgaa
     4081 atatttccct ttaacagaat taggcagaag tcgcccatcc tctataggt
//
LOCUS       DACDIE010000125         3952 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.128, whole genome shotgun sequence.
ACCESSION   DACDIE010000125 DACDIE010000000
VERSION     DACDIE010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3952
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.128"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..287)
                     /locus_tag="IV250_002735"
     CDS             complement(<1..287)
                     /locus_tag="IV250_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP8769964.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMIS"
     gene            complement(417..1001)
                     /locus_tag="IV250_002736"
     CDS             complement(417..1001)
                     /locus_tag="IV250_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8769965.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(1332..1565)
                     /locus_tag="IV250_002737"
     CDS             complement(1332..1565)
                     /locus_tag="IV250_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HAP8769966.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(1580..2530)
                     /locus_tag="IV250_002738"
     CDS             complement(1580..2530)
                     /locus_tag="IV250_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP8769967.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(2527..3369)
                     /locus_tag="IV250_002739"
     CDS             complement(2527..3369)
                     /locus_tag="IV250_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP8769968.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(3371..>3952)
                     /locus_tag="IV250_002740"
     CDS             complement(3371..>3952)
                     /locus_tag="IV250_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP8769969.1"
                     /translation="MLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKE
                     CVSMGTYAGMKVFQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCL
                     AQNADFIFLDEPFVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILL
                     NRNLIAHGSVESVFNEENLKKAYGDTIFIGEGE"
BASE COUNT         1341 a          727 c          479 g         1405 t
ORIGIN      
        1 catgagatca ttacgaccgg caacattcga aatacaatat ataaggatgt cgcaaaaaat
       61 atagtaaaaa actctatctt gatagcatca ataattttca ttactaaaaa cagaactgaa
      121 taaatcaaga tgaaataaat acatgtcttt attttacgaa acatcaatac taaaactgta
      181 gcaataatga aacaccccac ttgaaatata tttccctttc ctaaaaaaat aatgaccgga
      241 aaaaaaatat ttaaagtaat caaggttaac ggattgattt ttgacactct ttcacctcca
      301 aaaaataaga tcgcactgtt atcttaagct gataaaaatg caccgttatt cagataacag
      361 tgcgatctta tcaaaaagca taactgagaa caattctcaa tgcaagcttt ttttaattaa
      421 ctcagtaatt caatccaccc aggaacagaa aattgataca atcttctggt taatagcata
      481 gtcttttctt ttgaatagct atgcttcaat atatcataga atgtagcgta gaatgagtaa
      541 atgtagaagt ggatttcttc ttcattaaag actaaatttt gattgatcat tccgatagtc
      601 tttaacttat ctagacaaca gactgtcttt ttcgtcacaa atgatgtaat tttttcaaca
      661 atatcttgat atttggttcc agctgattgg aataaaatca aatcgaatat acgtttattc
      721 tcaaaaatat aattaataaa tggatctaat tcattgaagt tctctctcca acttttctct
      781 atatctatat tttctaagtt taagaggtat ttggtaaact cttcattata catattaata
      841 agcgcattaa tatgcggctg aataatttct tcaaataacg cttccttaga agtaaaatgt
      901 ttataaaaag cacccgtagt tacgcctgcg gtcaggcaca gttgccttat cttagtttgc
      961 tgaaatcctt ctttttcaaa atggcttagt ccactttcta ttaacttttg gtgagttata
     1021 atataatctc tggtttctat gggtacttcc tcctaataag atgaattaac tttattatct
     1081 tacacaggac ttgaagtttc aatcgttaac gtttattact gaactcattc cttagagcta
     1141 aactaatgag aaagagcaga ataactgata caaaaagcaa tcctacaaga attaatagac
     1201 tcatctaaat cattcacctt ttctaaaata atccgagaaa gatttaaaaa aattatatcc
     1261 agctattagg ataagtccta tcataatcac tccaatagcc attttcttat cctccctaaa
     1321 aataaagtct tttacttaaa accttttttg taatcgtcaa taaatttttt ccctttatca
     1381 gttaattgaa atcctatatc taaatagtcc tcatatctaa gtttagccag catctcactt
     1441 acagatgctg gactaacact tagaactttt gctaaatcat tattttttaa tttctttttt
     1501 ctttttgata attcctgaat tgctagtaaa taaattagta tagtagtcgt tttaatttgt
     1561 accataccta ccctccaaat tatttcgaaa ggccttcagc aattttatcc aggttccatt
     1621 tcatcatcgc atagtaacta tcaccatctt gtcctttttc tgcaattgaa tcagtaaaaa
     1681 tcgttgaata gataggaata ttggtatctt ttgatactgt tttcattggt ctatcatcca
     1741 cactgctttc tacgaataag gagggaactt ttgttgtgcg taatttttca actaagtgtt
     1801 ttatttgatc tggtgttcct tcttcttcag tgttgatttc ccaaatgtaa gcagaaggca
     1861 cattatacgc tttcgagaaa tatttaaagc atccttcact tgtaacaatc atttttttat
     1921 cattcggaat tgaagcaaat ttagatttag cttctttgtc tagagaatcc aatttttcaa
     1981 tatacttatc tagattttct ttatagaatt ttttattatc aggatctttt tccgctaact
     2041 gtttttcaat atttttagcg taaataatac cgttttctaa atttaaccaa gcatgaggat
     2101 cttccttccc tttctcactc tgaccctcta agtaaataac atctatgcca tcacttgctg
     2161 caaaatagtc tttgttttcc tctttgttcg catttttaac taattttgta aaccaagcat
     2221 ttcctccagt ttccaagtta acaccgttat aaaaaatcaa atctgctttt gaagtttttt
     2281 gaacatcttc aggcaaaggt tcatattcgt ggggatcttt cccaataggt acgatactgt
     2341 gtaaatcaat tttatctttt gctatatttt cggtaatatc tgctaaaatc gaattagtaa
     2401 ctactacttt taatttttcg ttcgaagcca ctttatcttt gtctttacta tttattgaac
     2461 atgccccgaa tataaaaaga gcaatcaaaa tccccagact tcccagtact gctttccatt
     2521 ttctcatcat aacgatctct ccttctttct taaaaatttt tgttttggtg agattagaaa
     2581 actaattaca aataagaatg ccgctgttaa aacaatactt gatcctgctg ctatgttaaa
     2641 actataacca ataaacaatc caagcaatga tgaacttgct ccgactgttg cagaaataaa
     2701 tatcatagtt ttcaagctat ttgcatataa atatgctgta gctgctggtg taattaacat
     2761 tgcaacaatt aatatagttc ccacactttg catagctgtt actgctacta aagttaatag
     2821 aaccatcaat agataatgat aaaaactgac attcatccct attgcttttg ccattaatgg
     2881 atcaaaagaa gtgattaata attctttaaa aaatagaact actataacca acactaaaac
     2941 cgaaacgcca attgtaatcc acttatcaat atcttgtaca gctaaaatat ttccaaataa
     3001 aatatgaaat aaatccgttg agctatttgc aactcctata aggataactc ctagtgctaa
     3061 aaaagaactg aaagtaatac caattgctgt atctcctttt attacactat tgctttttat
     3121 aaaagtaata atgatagaag ctaatagacc aaatacaatc gcaccaataa aaaaattaat
     3181 acctaaaata taagaaattg ctactcctgg caaaacagca tgagaaatag catcacccat
     3241 aagagacatt cctcttaaaa taataaaaca acctactgtt ccagaaacaa tccctataac
     3301 tacagatgta attagagcat tctgtaaaaa gtgaaagtca tacagaccat taataaaatt
     3361 ttgaagcata ttattccccc tctccaataa atatagtatc tccataggct ttttttaaat
     3421 tttcttcatt aaatacagat tctacacttc cgtgagcaat tagatttcga ttcaaaagaa
     3481 taatattatc aaaatacttc tttaccttgc ttaaatcatg atgcacaatt agcactgttt
     3541 ttccctgatg tttcaatttt tttaacgtat ccataattat tcgttcacta actaagtcta
     3601 tccctacaaa tggttcatct aaaaatatga aatccgcatt ttgagcaaga catcttgcta
     3661 aaagtacacg ttgaaattgt cctccagata attctcctat ttgatgatta gaatattttc
     3721 ccatatcgac cttttccaaa gcctttgaaa ctctttgcca ttctgcattt ttaattcttt
     3781 gaaaaacttt cattccggca tatgttccca tagatacaca ttcttttact tttattggaa
     3841 aagtaaaatc aatatcagct ttttgctcta catatgcaat cttcaccagt tttttattga
     3901 tatcttcatt attaatggag acctctccct gatgagggat aatatttaac at
//
LOCUS       DACDIE010000126         3862 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.129, whole genome shotgun sequence.
ACCESSION   DACDIE010000126 DACDIE010000000
VERSION     DACDIE010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3862
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.129"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..391
                     /locus_tag="IV250_002741"
     CDS             <1..391
                     /locus_tag="IV250_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8769970.1"
                     /translation="VMSEQLKNAGCVKDSFLEGITNREQEFPTGLEVNQIGFAIPHTD
                     SAHVNSSQICFASLKEPLVFSDMTDKSHEIPVRLVFMLAMSQPHEQIDTLQNLVSLFQ
                     NEEKVNELLACTTKEAFIELVHSAGVY"
     gene            405..1733
                     /locus_tag="IV250_002742"
     CDS             405..1733
                     /locus_tag="IV250_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAP8769971.1"
                     /translation="MDIVLNAFEWFIGLGSNVFLPLIIFVIGILFGLKPGKAFVSGVT
                     VGIGSIGLGLVLSLLSDGLGTAIQQMGEQYGGGLNILDIGVGVGGPLAYSTSLGILMI
                     PISLILNFIFVVLGWTKTLNVDIWNFWFAIFLGMITQSVTGNFWFGLIGAIVAVILQW
                     FLADRTQKTVSEFFGYPGISITHMMGLSGVLFAKPVNWVFDRIPGFNKIDADAETLTK
                     RFGVLGDTVVIGLIIGIVVGIMAGYDVAGIGNLGIMTAAVMKIMPKMVAMFMEGLMPI
                     AEAAKEFTNKKLNGRQVNIGMDAALTVGHPTIMSTSLLLVPISLLLALILPGNKVLPF
                     GDLAFFAFAICLMIPYFKGNIVRSLVGCSLYVVTTLYMATWLAPIITDVFTLASFDVG
                     TAGLVTMLLCGLWPVGLFVLLASYFGVIGIGGLGVVLVGASIYMNKVKKA"
     gene            1767..2051
                     /locus_tag="IV250_002743"
     CDS             1767..2051
                     /locus_tag="IV250_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8769972.1"
                     /translation="MKKLLVICGAGHATSTIAVAKLSKWLEEEGLTDQVKIYQSKIAD
                     ELNKIDEYDAVVSTTIVPDVIKAKVTNGLGLLTGIGTDAIYADVKERLGV"
     gene            2053..2568
                     /locus_tag="IV250_002744"
     CDS             2053..2568
                     /locus_tag="IV250_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HAP8769973.1"
                     /translation="MRFHHDTLTIETVSGRPSYHDIRKELLDTVDTAGIENGMLVISS
                     PHTTCSLFFEETMHDTNFFGDDYLHVDINNVMETIIPKMTSENQYNSPGPKHIDFGTR
                     LSDPNYPAEKWVMLNTDAHLRSSIYGTNSMTLIVQEGKLLLGEMGRVYFVDWDQLRER
                     TRTINLMIMGE"
     gene            2584..3246
                     /gene="rpe"
                     /locus_tag="IV250_002745"
     CDS             2584..3246
                     /gene="rpe"
                     /locus_tag="IV250_002745"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAP8769974.1"
                     /translation="MTIAPSLLNAPIAEVKELFEILKEKNVDYLHIDVMDGCFVPDMA
                     FGPSVIKDIRPLTDVVFDVHLMIDTPEKIVTRFVEAGADIVTFHIEATKHAMKVIQLV
                     KEAGAKVGITFNPATPVSFIEPVLSLVDQVLVMTINPGTENKHFIQETVVKIEQLDVI
                     RKQNDYTYDIEVDGKIDNQTIKVCSKAGADIFVSGGFIFNGKNTPQEQIDKLNQSLEE
                     VK"
     gene            3253..3825
                     /locus_tag="IV250_002746"
     CDS             3253..3825
                     /locus_tag="IV250_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8769975.1"
                     /translation="MTNKDQNKNYLEAIILELQNNSKRINSVELEQFSEKIVQAKHIF
                     LAGAGRSGIAMQAFTNRLMHLGFSVSLVGEISSPHSQPGDLLIICSGSGETGSLKNLA
                     AKANQSGVSIALVTMKKDSSIGQLADSVLVLPGITKDENQREEDTFSQPMGSSFEQLA
                     FLTFDSLVLSLMDKTGESSETMFARHADFE"
BASE COUNT         1287 a          561 c          783 g         1231 t
ORIGIN      
        1 agtaatgtct gaacaattaa aaaacgctgg ttgcgtgaaa gatagtttct tagaaggtat
       61 caccaaccgt gaacaagaat ttccaactgg attagaagta aatcaaattg ggtttgctat
      121 cccacataca gattccgcac atgtaaatag ctctcaaatc tgttttgcct cattaaagga
      181 accactggta ttttcagata tgacagataa atctcatgaa atacctgtac gtttagtctt
      241 tatgttggca atgtcacaac ctcatgaaca aattgataca ctacaaaact tagttagcct
      301 atttcaaaat gaagaaaaag taaatgaatt acttgcatgt accacgaaag aagcatttat
      361 tgagttagta cattctgcag gagtttatta ggaggaatag agagatggat attgtattaa
      421 atgcctttga atggtttatt ggattagggt caaacgtctt tttaccactg attatttttg
      481 taatcgggat attatttgga ttaaaacctg gtaaagcatt tgtttccggc gttactgttg
      541 gtatcggttc tattggttta ggattagttt taagtttatt atcagatggt ttaggaacag
      601 caattcaaca aatgggggaa caatacggtg gtggcctgaa tatcctagat attggcgtag
      661 gtgttggtgg acctttggct tactctacat cattgggaat tttaatgatt ccaatttcat
      721 taattcttaa cttcatattt gtggttttgg gttggacaaa aacattgaat gttgatattt
      781 ggaacttttg gttcgcaatt ttcctaggta tgattactca atctgttacg ggaaactttt
      841 ggtttggctt aattggcgcg attgtcgcag taattttaca atggttctta gcagatcgta
      901 cacaaaaaac tgttagcgaa tttttcggct atccaggaat ctctatcaca catatgatgg
      961 gattatcagg agtactattt gccaaaccag taaattgggt ttttgatcgt attcctggat
     1021 ttaataaaat agatgcagat gcagaaacat taacaaaacg tttcggtgtt ttaggtgaca
     1081 cagttgttat tggcctaatt atcggtattg tagtgggtat tatggcaggc tatgacgtag
     1141 caggaattgg aaacttagga attatgactg cggcagtgat gaagattatg cctaaaatgg
     1201 tagcaatgtt tatggaaggt ttaatgccaa ttgcagaagc ggcgaaagaa tttacgaata
     1261 aaaagttaaa tggtcgccaa gtaaacattg gaatggatgc agcgttaact gtgggtcatc
     1321 caactattat gtcaacttct ctattgcttg taccaatctc attattgcta gcactaattc
     1381 ttccaggaaa taaagtatta ccttttggag atttagcatt ctttgctttc gccatctgtt
     1441 taatgattcc ttattttaaa ggaaatattg tgagatcatt agtaggatgt tcgttgtatg
     1501 ttgttacaac attatatatg gcaacatggt tggcaccaat tatcactgat gtgtttacat
     1561 tagcaagttt cgatgtgggg acagcaggat tagtaactat gttgttatgt ggattatggc
     1621 cagtagggtt gtttgtttta ttagcatctt actttggtgt aattggaata ggtggattag
     1681 gagttgtttt agtaggtgct tctatctaca tgaacaaagt gaagaaagct taatcataaa
     1741 attaaaataa gaaagaagag atcaatatga aaaaattatt agttatttgt ggagcaggtc
     1801 atgctacttc tacaattgca gtagcaaaat tatcaaaatg gttggaagaa gaaggattaa
     1861 cagatcaagt gaaaatttat caatctaaaa ttgctgatga gctaaataaa attgatgaat
     1921 atgatgctgt agtaagtaca acaattgtcc ctgatgtaat taaagctaaa gtaacgaatg
     1981 gcttgggctt attaacaggt attggtacag atgccattta tgcagatgta aaagaaagat
     2041 taggtgttta atatgcgttt tcatcatgac actttgacta ttgaaacagt ttcagggcgt
     2101 ccttcgtatc atgatattcg aaaagaactt ttagatacag ttgatacagc aggtattgag
     2161 aatggaatgt tagtaatttc gagcccgcat acaacttgct cactcttttt tgaagaaacg
     2221 atgcatgaca caaacttttt tggtgatgat tatttacatg tagacatcaa caatgtaatg
     2281 gaaacgatta ttccaaaaat gacgagtgaa aatcaataca acagtccggg gccaaaacac
     2341 attgattttg gaacaagatt atctgatccc aattaccctg cagaaaaatg ggtcatgcta
     2401 aataccgatg cacatttacg ttcatctatt tatggaacca actcaatgac tttaattgtt
     2461 caagaaggta aacttttatt aggagaaatg ggccgtgtct attttgtcga ttgggatcaa
     2521 ctaagagagc gaactagaac aatcaatctc atgattatgg gagaataaac gggggaatag
     2581 agaatgacaa ttgctccatc attgttaaat gcacctatcg cagaagttaa agagttattt
     2641 gaaatcttaa aagaaaagaa tgttgattat ttgcatattg atgtaatgga tggctgtttt
     2701 gtaccagata tggcttttgg accaagtgtt attaaagata ttcgtccact aacagacgtt
     2761 gtgtttgatg ttcatttgat gattgataca ccagaaaaaa ttgtcacaag atttgtagaa
     2821 gcaggagcag atattgttac cttccacatt gaagctacca aacacgcaat gaaggtcatt
     2881 cagttagtca aggaagcagg agcaaaagta gggattactt ttaatcctgc aacaccagtc
     2941 agttttattg aaccagtatt atctttagtc gatcaagtat tagtaatgac aatcaatcca
     3001 ggaacagaga ataagcattt tattcaagag acagtagtta aaatcgaaca gttagatgta
     3061 atacgcaaac aaaatgatta tacgtatgat attgaagtag atggcaagat tgataatcaa
     3121 acgattaaag tttgttctaa agcaggggcg gatatttttg tatcaggcgg tttcatcttc
     3181 aatgggaaaa ataccccgca agaacaaatt gataaactaa atcaatcatt agaggaagtg
     3241 aagtaaaaga tcatgaccaa taaagaccaa aataaaaact atttagaagc gattatctta
     3301 gaattacaaa ataactcaaa acgtattaat tctgtagagt tagaacaatt tagtgagaaa
     3361 attgttcaag ctaaacacat ctttctagct ggtgcaggac gctctggaat tgccatgcaa
     3421 gcatttacga atcgcttaat gcatttaggt ttttcagtca gccttgtcgg tgaaattagt
     3481 tcaccacatt cgcaacctgg agatttatta attatctgtt ctggctcagg tgaaacaggt
     3541 agtttaaaaa atttagcggc caaagcaaat caaagtggtg taagcattgc tttagttacc
     3601 atgaaaaaag actcgtcgat tggtcaatta gcagattctg ttcttgtatt accaggtatt
     3661 accaaagatg aaaatcaacg agaagaagat actttttcac aacctatggg ttcaagtttt
     3721 gaacaattag catttctcac ctttgatagt ttagtgttat ctttaatgga taaaacaggt
     3781 gaaagctcag aaacgatgtt tgctcgacat gcagattttg agtagtagat tttaaagata
     3841 aaaaaccatt ctctaaatgc ta
//
LOCUS       DACDIE010000127         3647 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.130, whole genome shotgun sequence.
ACCESSION   DACDIE010000127 DACDIE010000000
VERSION     DACDIE010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3647
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.130"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            147..1169
                     /locus_tag="IV250_002747"
     CDS             147..1169
                     /locus_tag="IV250_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP8769976.1"
                     /translation="MFKDKILLITGGTGSFGNAVMKRFLDSDIKEIRIFSRDEKKQDD
                     MRKKYNNDKLKFYIGDVRDINSVKNAMYGVDYVFHAAALKQVPSCEFFPMEAVKTNIM
                     GTDNVVTAAIEMGIQKVICLSTDKAAYPINAMGISKAMMEKVFVAKARTVTDERTMIC
                     GTRYGNVMASRGSVIPLFIDQIKNGKPLTVTDPGMTRFLMSLEEAVELVVFAFQNAKA
                     GDIMVQKSPASTVGDLAQAIKELFHADNEIHIIGTRHGEKRYETLLTKEEYLVAQDMG
                     DFYRVPADTRDLNYDKYFVEGSKELTQEGEYNSDNTQRLSVEQIKERLLELDYVRKEL
                     KEWQNQ"
     gene            1166..2275
                     /locus_tag="IV250_002748"
     CDS             1166..2275
                     /locus_tag="IV250_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CapF"
                     /protein_id="HAP8769977.1"
                     /translation="MNILVTGSNGFIGKNLVAELKNEGFTDILSFDRDTPVHLLDEYC
                     EKADFVFHLAGVNRPKDDAEFMTGNFGFTSELLEKLKEHGNTCPVMISSSIQAELNNS
                     YGKSKKAGEDLLKKYQKETGAPVYIYRFSNLYGKWSQPNYNTVVATFCYKIARDEEVT
                     VNDPSAIIELCYIDDVVKELLHCLNGQPYTENGYCAVRETDEVTVGELKELITSFKNS
                     RTNLSVPNLARRVEKNLYSTYLSFLPEDEFSYFLKMNVDNRGSFTEFLRTKDRGQVSV
                     NISKPGITKGNHWHHTKNEKFLVVSGEGVIRFRKVDSNEIIEYFVSGEKLEVVDIPVG
                     YTHNIENLGSTDMVTVMWVNEPFDPEHPDTYYVEV"
     gene            2279..3403
                     /gene="wecB"
                     /locus_tag="IV250_002749"
     CDS             2279..3403
                     /gene="wecB"
                     /locus_tag="IV250_002749"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HAP8769978.1"
                     /translation="MKKLKVMTVVGTRPEIIRLSAVINRLDRSEAIDHILVHTGQNYD
                     YELNEVFFEDFKLKKPDYFLNAATGTAIETVGNILIKIDPILEEVKPDAFLVLGDTNS
                     CLCAIAAKRRHIPIFHMEAGNRCFDQRVPEETNRKIVDHTADINLTYSDIAREYLLRE
                     GLPADRVIKTGSPMFEVLNSRKDDIQHSDVLNRLGLKEGQYFVVSAHREENISSETNF
                     LHLVDSLNTIAETYQLPIIISTHPRTMKMIEAKEIKFHELIQTMKPMGFNDYNKLQIN
                     AKAVLSDSGTISEESSILNFKALNIRQAHERPEAMEEASVMMVGLEKERIMQGLEVLE
                     TQQKDTLRHVADYSMPNVSEKVLRIILSYTDYVNRVVWGR"
     gene            3400..>3647
                     /locus_tag="IV250_002750"
     CDS             3400..>3647
                     /locus_tag="IV250_002750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAP8769979.1"
                     /translation="MRIVVNDIAASEGGAMSVLRDLHSDIISSGDENEWIFLLGEKFI
                     EESDNIKVLTFPKIKTSWIKRLDFDFISGKKIINKLQP"
BASE COUNT         1331 a          563 c          740 g         1013 t
ORIGIN      
        1 taagtaatat ggagcgatta aaggatgaaa aaataagaaa cgaattaggt aaaaatgctt
       61 ttatttattt gaaaaataat tttgatgtaa aaagaagcta ttctataatt atgaatcatt
      121 ttgaattata aaaaggagca atttaaatgt tcaaagataa aattttatta atcacaggcg
      181 gcacgggttc ttttgggaac gcagtgatga aacgcttttt agattcagat atcaaagaaa
      241 tacgtatctt ttctcgtgac gagaaaaaac aagacgatat gcggaaaaaa tacaataatg
      301 acaaattgaa gttttacatt ggagatgttc gggacatcaa cagtgtcaaa aatgcgatgt
      361 acggcgttga ttatgtcttc catgcagcag cacttaaaca agtaccttct tgtgagtttt
      421 tccctatgga agctgtgaaa acaaatatca tgggaacaga taatgtcgta actgcagcaa
      481 tcgaaatggg gattcaaaaa gttatttgct tgtccactga taaagctgct tatccaatca
      541 atgctatggg gatttctaag gctatgatgg aaaaagtatt tgttgctaaa gcaagaaccg
      601 ttactgacga acgcacgatg atttgtggca cacgttatgg aaatgtaatg gcttctagag
      661 gatcagttat accattattc attgaccaaa taaaaaatgg taaaccatta acagtgacag
      721 atcctggcat gacaagattt ttgatgagtt tggaagaagc tgtagagtta gttgtttttg
      781 ccttccaaaa cgcaaaagca ggggatatca tggttcaaaa atcaccagct tctactgttg
      841 gcgacctagc gcaagccatc aaagaattat tccatgcaga caatgagatt catatcatcg
      901 gaacgcgaca tggcgaaaag cgttatgaaa cactactgac aaaagaagaa tacttagttg
      961 ctcaagatat gggtgatttc tatcgtgttc ctgcagatac acgtgatttg aattatgaca
     1021 agtatttcgt tgaaggaagc aaagagttga cacaagaagg agaatacaat tcagataata
     1081 cacaacgttt atctgtagaa caaatcaaag aaagacttct tgaactggac tatgtaagaa
     1141 aagaattgaa agagtggcaa aatcaatgaa tattttagta actgggtcaa atggttttat
     1201 tggaaaaaat ttagttgctg aattaaaaaa tgaaggattc acagacattc ttagttttga
     1261 tagagacacg cctgtacatt tattggatga atattgcgaa aaagcagact ttgtttttca
     1321 tttagctgga gtgaacagac caaaagacga tgctgaattc atgacaggaa attttggttt
     1381 tacttcagaa ttgctagaaa aactaaaaga gcatggcaat acatgtcctg ttatgatttc
     1441 ctcttctatc caagcagaac ttaataattc ttatggcaag agcaaaaaag ccggagaaga
     1501 tctattaaag aaataccaaa aagaaacagg agcacctgta tatatttatc gcttctcaaa
     1561 cttatacggt aagtggtcgc aaccaaatta taatacagtt gtagccacat tttgttacaa
     1621 aatcgcaagg gacgaagaag ttacggtaaa tgatccgtct gctatcatag agctttgtta
     1681 tatcgatgat gtcgtgaagg aactactcca ttgtctgaac ggacaacctt atacagaaaa
     1741 tgggtattgt gctgttcgtg aaacggatga agtaacggtc ggcgaattaa aagaactaat
     1801 tacatctttc aaaaatagtc gaacgaattt gagtgttcct aatcttgcga gaagagtaga
     1861 aaaaaatctg tacagtacgt acttaagctt cttaccagaa gacgaatttt cttattttct
     1921 aaaaatgaac gtagacaatc gaggatcgtt tactgaattt ttaagaacaa aagaccgcgg
     1981 tcaagtatct gtcaatattt ccaaaccggg aatcactaaa ggaaaccatt ggcatcatac
     2041 aaaaaatgaa aaattcttag tagtaagcgg agaaggtgta atccgtttta gaaaagtaga
     2101 ctcaaatgaa ataatcgagt attttgtttc aggagaaaaa ttagaagtag ttgatattcc
     2161 agtaggttat acgcacaata tcgagaatct gggatcaacg gatatggtga cagtgatgtg
     2221 ggtaaatgag ccatttgatc cagaacatcc tgatacttat tatgtggagg tctaatcaat
     2281 gaaaaaatta aaagtgatga cagtagtagg aacccgacca gaaatcattc gtttgtctgc
     2341 tgttatcaac cgtttggatc gatcagaagc aatcgatcat attttagtac acactggcca
     2401 aaactatgac tatgagctaa atgaagtatt cttcgaagac ttcaagctaa agaaacccga
     2461 ttattttcta aatgcagcaa ctggtacagc gattgaaaca gttggaaata ttttgatcaa
     2521 gatcgatcca attttggaag aagtaaaacc agatgcattc ttagtattag gggatacaaa
     2581 tagctgttta tgtgcaattg cagccaaacg tcgccatatt cctatttttc atatggaggc
     2641 aggaaatcgc tgtttcgacc aacgagtacc ggaagaaaca aatcgtaaaa ttgttgatca
     2701 tacagctgat attaatctaa cctatagcga tatcgcgaga gaatatctct tacgtgaagg
     2761 attaccagct gatcgtgtaa tcaaaacagg aagtccgatg tttgaagttc tcaattcaag
     2821 aaaagatgac atccaacatt ccgatgtcct taatcgctta gggttgaaag aaggacaata
     2881 ttttgttgtt tctgctcacc gcgaagaaaa tatcagctca gaaacaaatt tcttacactt
     2941 ggtagatagc ttgaatacaa tcgctgaaac ttaccaattg ccaatcatca tcagtacgca
     3001 tccgcgaaca atgaagatga tcgaagcaaa agaaatcaaa ttccatgagc tgatccaaac
     3061 aatgaaacca atggggttca atgattacaa taaattgcaa atcaatgcaa aagctgtact
     3121 aagcgacagc ggaacgatta gcgaagaatc ttccattttg aatttcaaag cattaaacat
     3181 ccgtcaagca catgaacgac cagaagcaat ggaagaagca agtgtcatga tggtaggatt
     3241 agaaaaagaa agaatcatgc aaggactgga agtattggaa acgcagcaaa aagatacttt
     3301 gcgacacgtc gcagattatt caatgccaaa tgtttcggaa aaagtattgc gtatcatcct
     3361 ttcgtataca gactatgtga atagagtggt gtggggaaga tgagaattgt tgtaaatgat
     3421 atcgctgctt cagaaggtgg ggcaatgtca gtattgagag acttacattc tgatataata
     3481 agtagtggtg atgaaaatga atggattttt ttactaggtg aaaaattcat agaagaaagt
     3541 gacaatataa aggtactaac ttttcctaaa ataaaaacta gctggattaa aagattggat
     3601 tttgatttta ttagtggaaa aaaaattatc aataaattac agcctga
//
LOCUS       DACDIE010000128         3606 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.131, whole genome shotgun sequence.
ACCESSION   DACDIE010000128 DACDIE010000000
VERSION     DACDIE010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3606
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.131"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(44..1510)
                     /locus_tag="IV250_002751"
     CDS             complement(44..1510)
                     /locus_tag="IV250_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAP8769980.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
     gene            1897..2493
                     /locus_tag="IV250_002752"
     CDS             1897..2493
                     /locus_tag="IV250_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP8769981.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(2551..3033)
                     /locus_tag="IV250_002753"
     CDS             complement(2551..3033)
                     /locus_tag="IV250_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769982.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(3139..3336)
                     /locus_tag="IV250_002754"
     CDS             complement(3139..3336)
                     /locus_tag="IV250_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769983.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
BASE COUNT         1232 a          717 c          581 g         1076 t
ORIGIN      
        1 ggaactgctg caaataagca attcctgttt ttatgttcat tgattaatga atagagccca
       61 ttagtttttt gattggttct ttcacagtaa atagcaagat accaccgacg attgctactg
      121 caccgataat accgaagtaa gcagattctg ttcccggtgt gtagaagcgt gcaatttgtg
      181 cattgattgc ttgtgaagaa gcatcagcta agaaccaaat agcgattgtt tgtgattcaa
      241 atgctcgtgg tgctaatttt gttgtcacag acaaaccaac tggtgaaaga cacaattcag
      301 caataaccac gatgaagaaa cttcccaaca accacaatgg acttactttt ccacttgtac
      361 cataaagcat acccggcaac atcaatagca agtacgataa tcctgcaaat aaaagtccaa
      421 gagagaattt cactactgta gatggttgac gttcaccaag tttcgtccac atcgttacga
      481 atactggtgt cagaataaag atgaaaattg gattcaatga ttgataccag cttgcttgga
      541 tcgttacacc aaatagcgta gattgtgtac gttcactagc aaataaagct aagatagatg
      601 aaccttgttc ttgtaatgac cagaaaatga ttgctgcaag gaataatggg atataagccc
      661 atactcttgg tttttcttca gcagttacat cttttgaagt cagcattttg ataaagtaat
      721 acaacggtaa gataatcccc aatacactga ttgtattgac aaaaaagtca attgtcagat
      781 gaccagttag ttgtgccccg ccgaatactg cagcgataac aacaagtgcg atgatcaagt
      841 tacggatgaa tcctttacgt tcttcagttg tcaaaggatt cggcggtgtt gtacccactc
      901 cgtctaatgt cttgcgtcct tggatgatgt attgtaataa tccaaagaac atcccaatag
      961 cagctaaaga gaaccctaaa tgatagttat attcttgacc gattgtacca acgattagtg
     1021 gtgctaataa tgcccctaag ttgacaccca tgtaaaaaat cgaaaatcct gcatctcttc
     1081 gcaagtcagt ctttgaataa agatgtccga ccatgccaga aacatttggt ttcaaaaagg
     1141 cggttccgat aataatcaat gccatcgaaa caaacaatgt acttacacca aatggtaaag
     1201 ccaagacgat gtggccaagc ataatgaaaa ctccaccgaa gaatacagtg cgttttgctc
     1261 ctaacactcg gtcagatacc catccgccga cgatactaga catgtagatc aaagatccgt
     1321 agatcgacat gatagctaat gctgttgctc gtggtaatcc aagtcccccg ttggcaactg
     1381 tatcgtacat ataatagata agtaatgcac gcataccgta ataactaaat cgttcccaca
     1441 tctcagtgaa gaacaaagtt gacaatccct tcggctgccc gaagaagccc ttgtcttgct
     1501 gtttttccat aatctatacc ccaattcttt aaattttcag aaaatattat acatctttac
     1561 cgaaaaattt aactaaaaaa cttatttaag aaatataaac taattttcta taaaagcaag
     1621 taatttttaa tggcaatatc ataaaaaaaa gaagatagag atttaataat tatatatcag
     1681 ctgttttttt caaataagca aaaattccta aaacacaaca atgaaagcga taactaagaa
     1741 aatccttaca taacgatttt tcttattaaa attgtttttt gaacttcgct tattttttgt
     1801 gagcatatct gtttacttac tttttgtaag gtgctatact tcatttcgta atcaattaaa
     1861 aaaatgcaaa acgcaaatca aaggagaaat tttattatga caacttttac tgacactttg
     1921 aaaaaccgcc gttcaatcta tcatctagga cgtaatgttt cactttctaa tgaagaatta
     1981 acaactttga tcaaagaagc aattaaagaa agcccaactg ctttcaatgc tcaatcaaca
     2041 cgcgcagtta tccttttcgg agatgcacat gaaaaacttt gggaaatcac tgaagaagca
     2101 cttcgcccat tgactccagc agaagctttc ccaaatacac aaaacaaatt agctggtttc
     2161 aaaaatggtt atggtacagt attattcttc aaagatactg acgtggtaaa aggtctacaa
     2221 gaacaatttg aattatatgc agacaacttc ccagattggt cagaacaatc aaacggaatc
     2281 gcaacagcta atacttgggt agcattagtt gataaaggat tgggcgcaaa cttgcaacat
     2341 tacaacccag ttattgacga agcagttgca aaagaatgga atatcccttc aaactggaaa
     2401 ctacgttcgc aattagtatt cggttcacca gaaacaccgg ctggggaaaa agaatacatg
     2461 aacgatgccg atcgcttccg cgtatttgga taattttatt tgaaataaaa aacagaggtt
     2521 gtgacaaaca tcttgtcgca acctctgttt ttattctgga tcaatcataa tatcaatctc
     2581 tgcctcatcg tttaagcgtt gggacaaaaa tggaactaat tcttctaatg atgcagaaaa
     2641 tttcagcatc ggtcctcgcc atccgtttcg gaaaaaattc atttttacga ttttagaacc
     2701 ttttggctga tataatacaa ttttattgat ctcagaaaaa ggaacacctt ttttgtcaaa
     2761 gctattcaaa acaagtcctt cttctgtgat tcctctactg tccaacaaaa aactaataaa
     2821 aatcattgct gacaagatcc cttttgccaa ttcatctgct gtaggatgac tgatgagtgt
     2881 ataccaaatg actagtgaca ctaagaaggg tatcaccaag tagacccatc gatttcgcac
     2941 agtccgataa ataatttctt ttcgaaccag ccaaagaaat aaaaacatca caacaacgaa
     3001 taaaaacaat agaaccgtaa agaatgttac catgacaact cgcctcgctt tttcttctcc
     3061 agaaaatagg aaaagaatac tgatttacct tctcaaagaa attggacttg cagcacgccg
     3121 cacgtccaat tgttttcttt attcaaatgt atcatatttt gcaacaacca tgcttgcttt
     3181 ttcatctaat aattcttggt cctctgcagc acattgggca actttgacca cggcgcagtt
     3241 tgtcattttt gatacaactt caccaatgac cgtttttgta aaaccaattg gtttgcatgc
     3301 atattgttct cctaaattaa tcatttcttc cgtcatcaaa atcctcctaa actgctggaa
     3361 cttttctcca cattaccact gctatttctt tggagattta actcattgat ttttatttac
     3421 agttaaaaat caaccttaca ggcattatag cgtactgttt tcgttttcac aattaaaaca
     3481 gctgttgtat ttagaagaat ttttttcaaa aaattaaaac atattcctta aaaactaatt
     3541 atttcctaca tattaaaata taatacttcc atttccaata aaaatacatt tataatagga
     3601 aagata
//
LOCUS       DACDIE010000129         3495 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.132, whole genome shotgun sequence.
ACCESSION   DACDIE010000129 DACDIE010000000
VERSION     DACDIE010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3495
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.132"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..473
                     /locus_tag="IV250_002755"
     CDS             <1..473
                     /locus_tag="IV250_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769984.1"
                     /translation="SLTLIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQ
                     EINPTTHFMSNKLTIELDNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTEL
                     TIPPGINKVVITYQYTKLAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(618..1358)
                     /gene="truA"
                     /locus_tag="IV250_002756"
     CDS             complement(618..1358)
                     /gene="truA"
                     /locus_tag="IV250_002756"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP8769985.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(1549..2184)
                     /locus_tag="IV250_002757"
     CDS             complement(1549..2184)
                     /locus_tag="IV250_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAP8769986.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(2331..>3495)
                     /gene="sstT"
                     /locus_tag="IV250_002758"
     CDS             complement(2331..>3495)
                     /gene="sstT"
                     /locus_tag="IV250_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAP8769987.1"
                     /translation="FFPTWTFISILGTLFVSCLKAIAPILVFFLTMASIAKHKIGNKT
                     FVKPILILYLVGTLLSALVAVIVSSIFTVPITLQETVSEKAPQSLESVLSTMLTNVTQ
                     NPVQSLIDANYLGVLFWAVLLGLAFRARSESTKELVDQISIALSQVVQVIIAFAPIGI
                     LGLVYQSIATTGIAGLAEYLQLLLVLIGTMLFVALVVYPFLTFLFIKENPYPLIFFCL
                     KESALPAFFTRSSAANIPINMMLAERLKLTKESYSISIPLGATINMGGAAITISLMTL
                     TAVHSLGIEAPFVLKVLLCILSALAACGASGVAGGSLLLIPLACSLFGISNDVAMQIV
                     GIGFIIGVIQDSMETALNSSSDLLFTAIGELGARKRSGEKIKLKDCLDGLSER"
BASE COUNT         1195 a          724 c          540 g         1036 t
ORIGIN      
        1 attcattaac acttatagca cctgaagacg ctgttgctat agctgaaaat tacaatcata
       61 ctgattatgc aaacaaagag tcaatctatc atcctgatat gattaataat gatcaatatt
      121 tactaggtaa ccaggaaata aatccaacaa ctcattttat gagtaacaaa cttacaattg
      181 aattagataa ttcgaataat aaaaatactg tcttgacgac acctatttat cgttataaag
      241 ggcaagtggc ttctattaat ggcaaactag tacaaacaaa attatctaag tttggcacaa
      301 ctgaattaac tattcctcca ggtataaata aagtcgttat tacttatcag tacacaaaac
      361 tagcaattgc ttcaagatat ttatctatcg ttactttgat tttattttta ctttatcgat
      421 ttactttttc taaacaaaaa caaccgagaa gagaagtaca acattctcac tagattttta
      481 tttaagaaag actttttagc tgtacacata aacaggctaa aaagtctttt tttatatttc
      541 tttaatttgc ttcatacatt atttaacatc gatatttata ctgtgaaaca tgcgtttcat
      601 ccattatcaa agtattttta tcgataataa acttcatcca aaaacagacc ttgtgcaggg
      661 acggtttctc ctgcatgttg ccgcactttg ctttcaaata tttcatcgat tgtttctatc
      721 ggcatggcac ctgtgccgat ttctagtaat gtccccatga taatccgcac cattttatat
      781 aagaatccgt ctccgacaaa agtaaaatga agcatttctc cctttcgttc gatagataat
      841 tcatcaatcg tacgcactgt ggattttttt gattttttca atgcagaaaa accaatgaaa
      901 tcatgtgtac ccactaattt ttcgcaagct gcattcagct tgtccatatc caactcttga
      961 gggaaataaa agctgtggtt tcgctcaaat gctgaaggaa tcacgttgtt ccaaacataa
     1021 tagctgtatt ttttgcccac cgcattatat cttgaatgga agcgctcagg catttcttcg
     1081 attcttttga tgacaatatc ccgcggaagg taacgattca taaagtcgat catttctgcc
     1141 agtccaattt ctgtgtttgt tttaaaattt gctacttgtc ctcgcgcatg ggtgccagca
     1201 tctgttcttc ctgatccaat aatttctatc tcttcatttg tcatctgatg caatactttt
     1261 tcgatttttc cttgaatggt tttatcgtca tctccaagtc tttgccatcc tgaatatctt
     1321 tttccgtcat attcgattgt caacttgatg tttctcatat tttagtggct cctctgtctt
     1381 ccatttggtc ttctctctac ctttttaaga ttgtagcaca aaatatagct ccttgcttct
     1441 ctaagacaga agctatacaa gaaaactgtg gaaatatata tttcagaaaa acaaaaaaca
     1501 gccaccggat gaagtatccg ctgactgttc aatttgacgg ctcaagtttt actgtttgag
     1561 atgcaagata ctcaaccagg caatgggcac ataattaaag aaaaaactga ttctttggca
     1621 aaaacctatt ttattcagta atggccaaga atttatagaa ggaattcttg ccagtccata
     1681 aatcgccgca aaaagcaaac tgatcacaga cagtccaagg tataagtggc ttacgtccat
     1741 ttgcccattt tttcgataga tcaagaaaag aagaactgga aacaataaaa aaccactgta
     1801 acctaaacct gatccgatat tatggaccca tgtcgagaca ttccacgaag attcatgggt
     1861 attgatactg aacaaacctg taaagataca atcacctata ccatagaacg cgatggctgc
     1921 tgtaactagg gtagctaaca cacgagatgt actaacaaat gcagcataca atgcaggcaa
     1981 tgacaggatg aaaaagatcc ctgaaaccac agaccaaata agaaacgctt ttcttacagg
     2041 actggaaacc tctccaaata cactgatcac gctcttcatt tgatctaatt caggataata
     2101 caatcctaat acgtacggag tcaaaaaatc actcagcacg cctaataata aaaagaaaaa
     2161 accaaattta cgtaaaaaat ccatgatacg cctccaataa caattaccta tgcttttaat
     2221 tgttattata aacgacttta tcttgattta acagaaacct ctatttacac gaacatctat
     2281 ataactcatt aaaaactcga atcattttaa aaatactaaa tatctaacgt tcaacgttca
     2341 cttaatccat ctaaacaatc tttcaatttg attttttccc cacttctttt acgcgcgcct
     2401 agctctccta ttgcagtaaa caacagatca cttgacgaat ttaaggcagt ttccattgaa
     2461 tcctggataa caccgataat aaatccaata ccgacgattt gcatcgcgac atcattagag
     2521 ataccaaaca aactacatgc taatggaatc aataacagtg aaccgcccgc aacacctgaa
     2581 gctccacaag cagctaaagc agacaaaata cataaaagga ctttcaaaac aaaaggtgct
     2641 tcaatcccta aagaatgtac agcagtcaac gtcatcaagg aaatggtaat cgcggcaccg
     2701 cccatattga tcgtagcacc taacgggatc gatatggaat aagactcttt tgtcaatttc
     2761 aatctttcag ccaacatcat gtttatcggg atatttgcgg cagaacttcg ggtaaagaaa
     2821 gcgggaagtg cgctttcttt caaacaaaag aaaatcaatg gatagggatt ttctttaata
     2881 aacaaaaacg tgaggaatgg gtacacaact aatgcgacaa acagcatcgt accgatcaaa
     2941 acaagtaaaa gctgcagata ctctgccaat ccagcgattc cagtcgtagc aattgattga
     3001 taaaccaatc ctaaaatccc tatcggagca aatgcaataa tcacttgtac gacttgtgac
     3061 aacgctatag atatctgatc caccaactct tttgtcgatt ctgaacgtgc tcgaaaagct
     3121 agtcccaaca atacagccca gaataagacg ccaaggtaat tcgcatcaat aagagattga
     3181 acaggatttt gtgtgacatt cgtcaacata gtagataaaa cactttccaa actttgcggt
     3241 gctttttcag atactgtttc ttgtaaagta ataggaacag taaaaattga acttacaata
     3301 actgctacaa gtgcagaaag caatgttccc actaaatata agatcaaaat aggcttcacg
     3361 aatgtcttat tcccaatttt atgcttggca attgacgcca tcgtcaagaa aaaaaccaga
     3421 ataggtgcga ttgccttcaa gcaactgaca aacaacgttc ccaatattga aataaatgtc
     3481 catgtgggga aaaag
//
LOCUS       DACDIE010000130         3381 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.133, whole genome shotgun sequence.
ACCESSION   DACDIE010000130 DACDIE010000000
VERSION     DACDIE010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.133"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..2232)
                     /locus_tag="IV250_002759"
     CDS             complement(<1..2232)
                     /locus_tag="IV250_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769988.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2904..3172)
                     /locus_tag="IV250_002760"
                     /pseudo
     CDS             complement(2904..3172)
                     /locus_tag="IV250_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          933 a          753 c          542 g         1153 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tatattaaaa gattcacttg tgctttcttc tggtacatta aaagactcac ttgtgctttc
     1861 ttctggtaca ctgatttcta ttgagaccgt atttgattga atatcaccgc ttcttgcggt
     1921 caattcgtat ttacctgaat gctgggaaga ataagtaatc agacgttcgt aatcattgag
     1981 ttgcttcact tccccttcaa tcaaatcctc actcttatca atgaccaatg catttagcag
     2041 gtctggtgtt cctctgactc gcaactggaa ctgctcaccc aaagcagcct cctcttgatc
     2101 aacttcgatc gtcagcgaac gctctggctg ttctgctaaa gactcagcta gaaccgttat
     2161 gttcccaaat tgattgatca caataagcat aactagaaac atacttaaca gtttatgaaa
     2221 tttgttcctc actattactc ccccttataa ttttgactaa acaacaaggt acccgataat
     2281 tttctaaaaa gattccccaa ctcaaaatgg ctgcgttacc tccctcgtat tttcggttca
     2341 actcaagcgt tgcaatgtaa gctgtaaaca aagctaatga ttgccgattg tcttctgttg
     2401 attgattcga tagctttgta tgacttttag ttgtccgtgt ggcttaacca taattatgat
     2461 ttcacgattc ctcctgccag tgccttgcac aagctggagc gtaaccctaa tagttatttt
     2521 gttaatgtac ttatatactt ttatactttt ccattagaaa caatcactaa atcgttgata
     2581 ccaaggtcag actctagaat ttcatcagtg agtccctgta ttggttaacc ttcatcaatc
     2641 ccaaaaaaat atatcaatgc ttagcatcaa aatgaatata tagtttagaa tacttgggat
     2701 tcatcgataa ctgatcgctt atttggccat attatttggc acttggttca actaaacgca
     2761 tttaggatgt tccttcattc tcgtcatgga tgttctcaaa ccgtcattac taataaactg
     2821 tccaccgaat cgatggttca tttcttgcat tttccacgtc caattactct ttttgctctt
     2881 tatttgggcg tagtttcact ttcttaactt gtatcgtctt ccatcaactc cttaatcatc
     2941 ttcttagctt tattggctcg gtttccttgt aaaaggtatc tacaaatagt gacaatctgt
     3001 attaaatctt catcattttc tgtaatgtca ataatttcta tattctgtac catatattgc
     3061 aaagggtttc actaagttca taaccgaagc gaattaaaca acttttgtat agaataacta
     3121 ccttttctat ttttccattg gtaattctat ctattaattg gtttaatcct tttgttgtag
     3181 ttgattccac taccaagatg attctctcga attttaaagc ttgaagatgt attgctattt
     3241 ttgctaactg gcgtatttgc aactattttt cccttctatt cttccttcat tgattcccct
     3301 ctaaaattac tatccataaa aaattgcata aatgacaata gtatttatat tagtcatact
     3361 gctttttaac acagcaaatt c
//
LOCUS       DACDIE010000131         3368 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.134, whole genome shotgun sequence.
ACCESSION   DACDIE010000131 DACDIE010000000
VERSION     DACDIE010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.134"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(99..1229)
                     /locus_tag="IV250_002761"
     CDS             complement(99..1229)
                     /locus_tag="IV250_002761"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAP8769989.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     gene            complement(1878..3080)
                     /locus_tag="IV250_002762"
     CDS             complement(1878..3080)
                     /locus_tag="IV250_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8769990.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="IV250_002763"
     CDS             complement(3140..3343)
                     /locus_tag="IV250_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP8769991.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
BASE COUNT          982 a          687 c          518 g         1181 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactc
//
LOCUS       DACDIE010000132         3161 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.135, whole genome shotgun sequence.
ACCESSION   DACDIE010000132 DACDIE010000000
VERSION     DACDIE010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3161
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.135"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            63..296
                     /locus_tag="IV250_002764"
     CDS             63..296
                     /locus_tag="IV250_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769992.1"
                     /translation="MDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVFVYLVCSYL
                     LKRAGKDNSFWRLTKVTVAATAVILTAILPLYM"
     gene            complement(455..577)
                     /locus_tag="IV250_002765"
     CDS             complement(455..577)
                     /locus_tag="IV250_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HAP8769993.1"
                     /translation="MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK"
     gene            767..991
                     /locus_tag="IV250_002766"
     CDS             767..991
                     /locus_tag="IV250_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769994.1"
                     /translation="MIKTQPLLVRFTLRTSHEPINCVVANIEIADKLLILEEGKYTIS
                     VTGHFNKRKQLVIESMQIRNPDHFTRSMSI"
     gene            1434..1640
                     /locus_tag="IV250_002767"
     CDS             1434..1640
                     /locus_tag="IV250_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAP8769995.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYCLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            1640..1891
                     /locus_tag="IV250_002768"
     CDS             1640..1891
                     /locus_tag="IV250_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769996.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEVLLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENLETYENEYWLTKLEN"
     gene            2072..2344
                     /locus_tag="IV250_002769"
     CDS             2072..2344
                     /locus_tag="IV250_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769997.1"
                     /translation="MNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQ
                     ALELTYAKFIETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            2789..2968
                     /locus_tag="IV250_002770"
     CDS             2789..2968
                     /locus_tag="IV250_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8769998.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDVPALKK"
BASE COUNT         1108 a          530 c          625 g          898 t
ORIGIN      
        1 gatgatctac cgttttagtt tgagaaagaa ggagtaagca tggcaaaaaa attagtctga
       61 cgatggatct attgataccc gtaattgcat ttatagacgg gatttctttg ggcgtcactg
      121 aaaatccttt ggtaaataga attgcgaaag gatcactatt tcttgtattc gtgtatcttg
      181 tctgttctta tcttttaaaa agagcaggaa aggataattc cttttggcga ttgactaaag
      241 tcacagtagc agcgacagct gttatactta cggctattct accgctttat atgtagagtt
      301 aacgggaaat cgttgaactt cttgcgaaat attgctataa tgaacatgaa aaagaggagt
      361 tgcgctaaca acccctcatt aacagcaccg attaaagaac ggtggcagaa tattagttat
      421 ttggaaataa ccgctgacct gctaaagtag acggttattt ctttttgtca tttttaagca
      481 tatttacaat caaactaatc aaagcaatgg taaacatacc aaaaccaaga atggtttgaa
      541 ttgtctcata tgcggacaaa aggcttactc ctttcataga ttttagcact tacattcata
      601 agcaccacca cctttccaga agtcgccacc gtcttcaaac tttactgtct tgactattat
      661 agcatatcta aaatcgaata aaaattctca attttagctg tttttgctat aattttagcg
      721 aggtgatcgt gatggaacaa tatattggtt tactcgataa attagggtga tcaagacaca
      781 gccattgctt gtgcgattta cattacgcac gagtcatgaa ccaatcaatt gtgtagtggc
      841 caatatagaa atagcggata aattgctgat tttggaagaa ggcaagtata ctatttcagt
      901 gacaggtcac ttcaataaac gaaagcaact agtcattgaa tcaatgcaaa ttagaaaccc
      961 tgatcatttt acgcgatcga tgagcatata aaagaagagc tgcagtccta aatgaggatt
     1021 gtagctcttt ttgttttaca tagaaacagt aaaaatgaaa aggcctgttt aggaaggcac
     1081 aagcgccttc tcaattgaaa gacctttctc tcactccaac cgccaccgcc gttcgagaag
     1141 aaaggaagtg aacgctacgc gttcactaag ataaagggtc ttgcatagca agacaaaata
     1201 gaaaaaattt cctctcacca aaaggttcgc tcctgttcgt tatctcttta ttgatttgtc
     1261 agaccggcaa caagttgcct tttatcacca aaaagcgaca aggacaaggg taaatggcac
     1321 gctgcgcgcc cttgtcttgt cgcttttcgt tgattcattc tggccaacaa atcaataaag
     1381 aaagaactaa cgaaaggaag taaaaccttt cctaagagga ggcagaatca atcatgcgaa
     1441 agaaatttga ttattggggt gttcccttca gcgaattatt cccaaactat catgcaccac
     1501 atactgtcga atgcgattgt ggagaaagag cgaaatgtat caaaagctat tgtttgtatc
     1561 aatgtccaac atgcgggaaa aaatatacct tgtcttatgg cgattatatt ttaattgatg
     1621 agaaaggaaa gaaacgctga tgaaaacaca acacaaatta ttgactgtaa aagaagtcgt
     1681 tcaaattttg gtggacaaag aagtcatttc tgaaaaagta gcagaagttt tacttattcc
     1741 gcggtactgg acgatagaaa cccctttagt agaggatgca ccgactatgc tatttgaaga
     1801 cctagtgaag attgcaggat atgaactgca ttatcaagaa aacttggaaa cttatgaaaa
     1861 cgaatattgg ttaaccaaat tagagaacta aaaaggagaa aaaaatatgg ctcaaaatta
     1921 ctatgacgaa tttgtcaaat tacctttgga taaaatggca caaaaaatgg aggatatgac
     1981 gttcctatac aacgaaacac gtgttccaaa aaaacattat aaagaaaaat tgtcggtagc
     2041 tgtggaagaa atgattgaat aaggtgtcga aatgaatctt attgcgacgt actatcgaac
     2101 actggaagaa ctgaaaaagc aaaatgcaaa atggtttttc caagccttac tatgcttgga
     2161 agtaggtgtc aaaccaagca ctattaaacc ttctgaatac caagctttag aactaacgta
     2221 tgcgaaattt atcgaaacga aaaaagccaa gactgtttca agtgaatggt tagattattt
     2281 tgaaaatatc aataagtatg gcgcatatta caccatgaaa aaggaagaca acgaaaatga
     2341 ataaggtgat ttatcaacta acaacggatt ccatatcaac gatttgaaag aaaaaaaggt
     2401 caataatcat ttataccgaa aagtgagaat gtgtgaaaca aggacaaaaa aagcctagta
     2461 actaaggaaa tggttcttat ttcgcaccaa tcgctccaac cgcccccgcc gcttaggtgg
     2521 tgcgaaataa acgggcaaag cccgataact taagagaaag aaggtgatcc atttgaacaa
     2581 gattattccc ttattgttag tactgtttgt tggcgaaata cttatgcgag atagtgcagc
     2641 tgtacagtta aacctcgctg tatttttggt aatactggtg ttttgtttga tcttttggcg
     2701 gtttgtatta gtcctcgtga gtctactatt caagatgatt gcagccatat ttttcatagg
     2761 gttatttcga tgaaaggagt gagtaccaat gccaattgca ataggaaata aacggttacc
     2821 cgtgacatta gatgaaaaga gacaaaaaga attgcagcaa ctaaagcaga agtatggcaa
     2881 aagtgaatct aagattatgt gtattgcgtt agatctattg atcgcgcaag aaaaagcagg
     2941 atttgatgta ccagcgctaa aaaagtgacg tcacttttta atttgatcta tataagtgat
     3001 atcactttta ccaaggaggg aattaaatgt taaaaactaa aaagaattcg tacaaattaa
     3061 agcttagtat actatctatt atttgtcttt taggcatgat aattagtggt catttgtata
     3121 gagtggcata tacgcagttt attgagtaca aggttcactc t
//
LOCUS       DACDIE010000133         3149 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.136, whole genome shotgun sequence.
ACCESSION   DACDIE010000133 DACDIE010000000
VERSION     DACDIE010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3149
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.136"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(368..982)
                     /locus_tag="IV250_002771"
     CDS             complement(368..982)
                     /locus_tag="IV250_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8769999.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1588..1965)
                     /locus_tag="IV250_002772"
     CDS             complement(1588..1965)
                     /locus_tag="IV250_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770000.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2265..3146)
                     /gene="galU"
                     /locus_tag="IV250_002773"
     CDS             complement(2265..3146)
                     /gene="galU"
                     /locus_tag="IV250_002773"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP8770001.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          958 a          652 c          488 g         1051 t
ORIGIN      
        1 gcaataatga taacaaatgt actataatta ctttgtaaag agttttgggg aagactcttt
       61 catcaacgtg tattaagaga caagaaagaa tccatatgga ttctttcttg tctctttttc
      121 cactactttt tatttttctg acactacata ttcttataac tcatttctca taactgtgaa
      181 acaacttata aaaataacaa actttaaaca aaacaagctt aaaatcaaag atgatccatc
      241 ttaaaataac taaaacaaaa aaagtagtcg ttgttaatcc tcattaataa tcgttttctt
      301 tttttcaaac gctccatttt tatgtggaac attatttttt taaggaactt tgtcaacatg
      361 tggataacta ttttccactt gtgtcctgga ctagctgttt tgctgtaaaa tacaaaactt
      421 gctgcagcaa aaagatcgtt gcaagagagg ctgttccgca gatcgctgga tctagatttg
      481 gcaaagtcaa gactgcatca tgtaaattgg cgtggttgtc caaaaccaaa tcagcaaatt
      541 caaataatcg caaaccactg ctatgttgcg atttgatact tcgtgacaaa tcaaatccag
      601 taacaatgat taatggatga ccattttctt tgatccactg agctagttcg atgattgccg
      661 ggtttcttcc ttcattcgaa agaagaagaa aaatgtcttc tgatttgaca gaaagctgat
      721 caataattgc ctgtcctgca ccttctagcg tttcatagac acctccggct ggattttcaa
      781 tcaatatact tggtacaaga tccggggttt gatcccaaaa ttcaaatgct acaccactta
      841 gtgtgcggct agcatatatc tgcaatctac caccttgatt aatttgtttc gcgatccaag
      901 tgccagcttg aagcatcagc ggctcttcta tccttaattt ctcttccaat tggtgataat
      961 agtcaaaata acataaactc atttatctct cccgcctagc tatacttgac atgaatagaa
     1021 acagctgcct aatctaagga agtacataaa aaaacagtag ttagacagta acacatgtgg
     1081 tctttgctgt ttcaaacaca aaaaaggcat tgaacgaata cgcccaatac cttgacatag
     1141 caaataaaac aacgtgcaga ccatggaaaa tatctacata ggggtagaat tttcacccta
     1201 gattaggtac aataaattag gtcgatgcac tgcattgact atggcaaaat gatcggtcag
     1261 ggtgtatgtg tgtgactggt cattccctga ttcttagcgc caactaaggg tcagggagcc
     1321 tttttttatt cagctgtttc tatctgaatt tattgtaagt aatctcttat ccaagtgcaa
     1381 gcaagaaaat acaaaaaaac attggtatga tttatatata tataaataac ataaaaaaag
     1441 gaatggctta tcccgataca acaagcattc ctttaaaaaa gattctttta tataagtgaa
     1501 aaaagctttt ttccaccaat taaataaatc tctcatctca atagtttatc cattatttta
     1561 tctttttttg cgtataccta ctaaatatta ccgtcttctt ctcgttcttc tactttgttc
     1621 gagagaatct gtactagaaa aagtagttgt atgtctacct tttctgccta tccgagactt
     1681 tcttttcata tatgggatat aaatgattag atacagtaca gcaaaaaata aagcaaatac
     1741 gcaagcttcc caactaccta acgccaattc aaccatcaag gtagcaaccc caacggatgc
     1801 tgcataccag ataaaaatca gaaacgtttc tttttttctg accatgcgaa acatgataaa
     1861 tagactaata agcaatagtg gcaacacaaa tatccaattg cttccttcca tcagccactt
     1921 gagtatatca agcagcgact gtgcaacagc gactaatttt cccatagtga acacacacct
     1981 ttttttataa ttaaaaaatt cgccttttta ttctactatg aatcttctgg cttggctttc
     2041 atttctctta tgtaatcatt ttcttttata caacttaaaa aaaaattaaa ccattagcat
     2101 aatattttct gaatctattc acgaaagtct ttttctatct ttcgttttcc acgactcaac
     2161 tgatctagag tcaaaaaact aaaacgacag ttgcgcaaat gacgcacaag ctgccgcttt
     2221 aataacaaat agcctttcat tctcagcagg ccacttattt ccagttaact ttctgttctt
     2281 agctttttac tcaactggat aatatatgca cttaatgaag tcccaatttc cgaatgttga
     2341 agtccatatt caatatttgt cttcatatat cctaacttat cgcctacgtc gtatcttttt
     2401 cctttaaact ctctcgcata gaccgcctct gtctggttaa gagtctgaag tgcatccgtt
     2461 agctgtatct cgtttcctgc tcctggttct tgagtttcta atatctcaaa tattttagga
     2521 gtcagtagat aacgtccgat aatggctaaa ttacttggtg cttttcccat ttcaggtttt
     2581 tctacaaacc cttttactct gcaacgaccc ttttcgctat ctgttttagg atcaataatt
     2641 ccgtatttag atgtttcatt ttctggtaca cgcatgacgg caacagcgga agcttgtgtt
     2701 tcttcataat ccatcatcaa ttgttgagtc aatgggatat catctttcat caaatcatcc
     2761 cccagcatga cgacaaacgg ttcatttcct ataaatgctt ttgcttgaag cacggcatcc
     2821 cccagtcctc ttggatgaga ttgacggatg aagtgaagat tgacttgagt cgtttcttca
     2881 actaaagcga gtaattctgt tttttccttt tcccgcaaat tgttttccaa ttcgatattt
     2941 gcatcaaagt gatcttcaat gggtcttttt tcttttcccg ttacgatcaa gatgtcttcg
     3001 attcccgaat ccaatgcttc ttccacaata aactggatcg ttggtttatc cacgattgga
     3061 agcatttctt ttgccatggc ttttgttgcc ggcaaaaaac gtgttcctag cccagcggca
     3121 ggaatgactg cttttttgac cttcacttg
//
LOCUS       DACDIE010000134         3136 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.137, whole genome shotgun sequence.
ACCESSION   DACDIE010000134 DACDIE010000000
VERSION     DACDIE010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3136
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.137"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(554..2167)
                     /locus_tag="IV250_002774"
     CDS             complement(554..2167)
                     /locus_tag="IV250_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAP8770002.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            complement(2308..>3136)
                     /locus_tag="IV250_002775"
     CDS             complement(2308..>3136)
                     /locus_tag="IV250_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8770003.1"
                     /translation="LSAAPYFRLLINESANLYAEFAHKSIELDNPSLLFIDKQQEVQI
                     FSTQKETQTFIIIQFYFDEQLLAHITGQSFSIDQEITSIITFLKNEQFKIDYLKHSKP
                     AISYDQEINSFFILCQQMSIHLLSLLVTYCLQKIQLSSLPRHYQQELALLTIPSNEKV
                     IPKKKHILYSDELVDPIIRYLEEHLSENFLLEQLAQIFFISPASLKKHFRKVNGKSVI
                     SYFKELKMEKAKRLVLENELTYTEIADILGFHSIHHFSSAFKKYTGLSPSKYYLSLK"
BASE COUNT         1014 a          546 c          550 g         1026 t
ORIGIN      
        1 tgttaaataa ataggaacaa aaaaattaaa ttatttaata cacacattag ggatcattca
       61 ttgaaggatt agaagacaac gaggatggcg aaaaatatga ttacaataga aaagtcgttc
      121 taaaccaatc agttcctagt atcacacaac gcaagccagt cgatttggtg agattagagg
      181 agtgaaagca aactttatta tataagaaaa aatattcgaa agggatgcta gtttaggtac
      241 tagtgctgat agattaatat gatgcctcgt aatattaggc gtagttactc tagagacatc
      301 actatggact atgatttgtc tcagagtcac aacatgtttt tttatgaaaa gaaacagcta
      361 aaataattaa attgcgcaat gtttaaactt tcttaaacta taacctaata aatgtgaaga
      421 aataatgcac aatttcttca catttatctt ctattctata tacataccaa ccaacaaccc
      481 tatatacttt ttcattttta ctcctcaagt aaaaataact cctagaccgc ggctacccac
      541 cgcggtcgtt tttttattct tcaatcaaaa tcgctttgat acccaaacct gccaagattc
      601 gggcagaagt ataagtattc aatcctcttc tgaaagtaat gtaaactttt ttatcttttg
      661 ggatttcatt gattcgatct cgtaactcat tcattggaat attttttgtt gccgagatac
      721 tgccagaagg tgctttatta ggttcacgta tatctaaaaa gaacgcttct ttcaaatcgt
      781 tttcgggtat atcacttgct ttgatcgttt ctacgatatt cgtcatttga ttgattgcga
      841 cataaccagc tatattcaac gggtcacggg tagtagaata cggtggagag taaggcaact
      901 ccaaatcagg caaatcaaag actgtcaagt ttccggtgat tgctacagaa agttctccca
      961 tccgtttatc gacacctttt tctccaaatg cttgtccacc taaaatacga ccgctatctg
     1021 catcaaaaat cagctttaga tttaatcttg tagcttctgg atagaaatag gcatggtcaa
     1081 agggagtgat aaaaacagtt ttataatttg ttatgcctga tgcttttaat tcatgttctg
     1141 tcattcccgc ataacttgct gtatgatcaa agatttttgc gacacctgca ccaatatatc
     1201 cgcgatagcg cataggcgta ccatttaaaa tatctgccag catatgtcct tgacgattgg
     1261 ccgcactgga aagcatactt tggatcggct gaccagtcac tacactagtc gtttcgataa
     1321 tatcgccaat cgcatagatg tccggcagat tggtttgtaa ctggtcatca acaatgatct
     1381 gacctgtatc tgataattgt atgccggttg ctttcaccac ttcattcttc ggagaaacac
     1441 caacagcaaa aattagcata tcagcagaaa ggacggagcc atctgataat actatttctc
     1501 gacctttatc acggatctca gtaactcttg tttctaaatg aacggctaat ccttctttca
     1561 gcaatttgtc ataaactaaa ttagcaattt ctggatcata agggaaagcg acttgtggca
     1621 attgatcgat taaagtcacg tctaagccgc gatgcttgaa attctcagct aattccagac
     1681 ccataacacc tgcaccaaga atgacgacat gttgtggatt tttttcttca aggaacgatt
     1741 tgattcgatc cgcatccgtc acactacgga ggacaaagcc gttttctgct tcaaaaactc
     1801 cgggaatatc cggataatct ggtctagcac cagaagagat gatcaactta tcgtaatctg
     1861 tttttatttc ttcatttgtg gataggtctt taactgttaa tcgcttagta gaaggatcga
     1921 ttgctgttac ttcgtgttta gtaaagacat cgatgttgtt ttttgttttc aatatttctg
     1981 gtgtacgttc gatcagcgag tcacggtcag tgatcacacc tcctaaataa taaggcaaag
     2041 cacaacttgc ataagaaata ttttctccgc gttcatagat aatgatttcg tgcgcttcat
     2101 tcaatcgacg gaaacgagtg gcaaatgaag gaccgccagc tacaccgcca atgacaacaa
     2161 ctttcataaa aacatctcct tttctacgtt gatcgtacta tgttatggta aaaaacagca
     2221 aataattgtg ccggaaaatt attgtttatt ttgaatagac agaaaatata gagaaataat
     2281 ttgtggataa aacctaaaaa tttttttcta ttttaaggat agataatact ttgaagggga
     2341 taaccctgta tattttttaa aagctgaaga aaaatgatgg atcgaatgaa atcctaatat
     2401 atctgcaatt tctgtatagg ttaattcatt ttctagcacg agacgttttg ctttttccat
     2461 ttttagttct ttaaaatagc taataacact ttttccattc acttttctaa aatgtttttt
     2521 taagctggct ggactgataa aaaaaatttg ggctaattgt tcaagcaaaa aattttctga
     2581 aagatgttct tctaaatatc gtatgattgg atcaactaat tcatctgaat aaagaatatg
     2641 ctttttttta ggaatgactt tctcatttga aggtattgtc aataaagcaa gttcctgttg
     2701 ataatgcctt ggtaaggaag acaattgaat tttttgcaga caataagtaa ctaataagga
     2761 aagcaaatga atgctcatct gttggcatag tatgaaaaaa gaattgattt cctgatcata
     2821 ggaaatagct ggcttactat gttttagata atcaattttg aactgttcat ttttcagaaa
     2881 agtaatgata gatgttattt cttgatctat agaaaaagat tgtcccgtaa tatgtgctaa
     2941 taattgctca tcaaaataaa actggataat gataaaagtt tgtgtttcct tttgagtaga
     3001 aaagatttgg acttcttgct gtttatcaat gaataaaagg gaaggattat ctagttctat
     3061 agatttatgt gcaaattcag catataaatt agcgctttca tttattagta gtcgaaaata
     3121 gggagcagca gataaa
//
LOCUS       DACDIE010000135         3029 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.138, whole genome shotgun sequence.
ACCESSION   DACDIE010000135 DACDIE010000000
VERSION     DACDIE010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3029
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.138"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..657
                     /locus_tag="IV250_002776"
     CDS             <1..657
                     /locus_tag="IV250_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP8770004.1"
                     /translation="KGLIVSCQALPGEPFYKEEESLMPYFALAAQRAGAVGIRANSVR
                     DIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIALDCTLRNRHDG
                     LSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGYTEESKKQDGPD
                     IDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQEIASRFVKALS
                     "
     gene            671..2173
                     /locus_tag="IV250_002777"
     CDS             671..2173
                     /locus_tag="IV250_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8770005.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            2186..2662
                     /locus_tag="IV250_002778"
     CDS             2186..2662
                     /locus_tag="IV250_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP8770006.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(2759..2869)
                     /locus_tag="IV250_002779"
                     /pseudo
     CDS             complement(2759..2869)
                     /locus_tag="IV250_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          994 a          411 c          596 g         1028 t
ORIGIN      
        1 aaaggtttga ttgtttcctg tcaagcatta cctggtgaac cattttacaa agaggaagaa
       61 agtttaatgc cttattttgc tttggctgct caaagggctg gagcagttgg tatacgtgca
      121 aattctgttc gagatattat tgaaattaga aagtgtgtat cattaccaat gattggaatt
      181 attaaaagag attatccacc agaatctcca ttcattactg ccactatgag agaagtagat
      241 gaattagtag aaaccggtgt tgaggtaatc gctcttgatt gcactcttag aaatcgacat
      301 gatggactat ctataaattc ttttattact aaaataaaag aaaaatatcc tcatcagtta
      361 tttatggcag atatttctac atttgaagaa ggcaaaaatg catataaagc aggaatagat
      421 tttgtaggaa ctactttaag tggatatact gaggaaagta aaaaacaaga tgggccagat
      481 atagatttga ttaaaaaatt ggtagaagaa gggatttgtg taattgctga gggaaggatt
      541 catactccag aacaagcaaa agagataaaa aaaataggag ttgcaggtat tgttgtgggt
      601 ggtgctatta ctcgaccaca ggaaattgca tcaagatttg ttaaagcatt aagttagggg
      661 gagaaataaa atgtttaaaa aattttcaca attaggacgc gctttcatgt taccaattgc
      721 tatattacca gtagccggat tactattggg actgggagga gcgttaacaa atgagagtgc
      781 tataaatgct tatccatttt tagatcaacc gtggttgcat acaattttaa gtattatgag
      841 ttatgctgga aatgcagttt ttgcaaacct agcactaatt tttgcaattg gtattgctgt
      901 aggacttgca aatggagata aaggaacagc tggtttagct ggtggagtag cttatctagt
      961 atatactgca actattagtg ggtttcttga acttttttca gcaaaagatg caacactaga
     1021 tacaggtgta gtgggatcaa ttgttgttgg tggtactgtt gcatttttac acaatcgtta
     1081 tagaaaaatt gaattaccac aattcttagg tttttttgga ggatcaagat ttattccgat
     1141 tgtttctagt ttagctgcaa tcattattgg ttctttcttt tatttaattt ggccaccaat
     1201 tcaaaatggt ttagtagttg ctggtgaaca aattgcccaa atgggtagtt taggaacttt
     1261 tttatatggt tttttattgc gtttaacagg tgctgttgga ctacaccaca ccatttaccc
     1321 tttgttctgg tatacatctt taggtggtac cgaaactgta gctggatcaa caattgctgg
     1381 agcccaaaat attttctttg cccaactagc tgatccaaac cacactggtc tgtttactta
     1441 tggtactaga ttttttgcag gaagatttgc aacaatgatg tttggattac cagcagcatg
     1501 ttatgcaatg tatcgtgcaa ttcctaagaa aaatcgtaaa aagaatggtg ggctttattt
     1561 tagcggggcc cttacatcat ttttaactgg aattactgaa ccagttgaat atatgttttt
     1621 atttgttgct ccttggcttt atgtgattca tgcattctta gatggattat ctttttattt
     1681 tgctgatatc ttaaatattc gtgtagggaa ttccttttca ggtggtttga tcgacttttt
     1741 attattcggt gtacttcaag ggaatgacaa aactaattgg ttaaaattaa ttccttttgg
     1801 tattgcatgg gcatttatat atttctttgt ttttacattc tttatcaaaa aatttaaggt
     1861 agctattcct ggaatggaag aagaatcaga agttcattct gaaaaagtaa taaacaaaaa
     1921 tacatcatta catgaagagt ctcttatcat tattgaagct cttggtggaa aagaaaatat
     1981 tgagtctgtt gaagcttgtg caactcgttt gcgtgttgct gttacgaatg gggagattat
     2041 tcaaaaacaa acaataaaag acactggtgc aactgctgtc ttcgaagtga agggtggcat
     2101 ccaagctgtt tttggaggga aagctgactt attaagtcaa gaaatcaatc aaatattagg
     2161 aaaagataat taggaggttt ttaatatgtt tgaaattttc aaaaagaaaa aacaagaaac
     2221 tttttatgca ccttgttccg gaaaaatcat ttccatcaca gaagtaaatg atcctgtctt
     2281 ttcacaaaaa ttactaggtg atggttttgc cgtcatacct gatggttcaa aaatttatgc
     2341 tccattggct ggtcgagtaa caaatatatt tcctactaaa catgctatta atataaaaag
     2401 caactcaggc attgattatt tgattcatat tggaattgat acagttgaat tacaaggaga
     2461 gcctttttcg atttgtgttc aagaaaatga tgaggttact cctgaaacct cgttggttga
     2521 tgtggatttt gacaaaataa taaaatctgg taaagatact tctgttatag ttgtttttcc
     2581 ggagaaaaat cagttcaatg atttaataat agataatggt cgaattgctc atgggaattc
     2641 atgtggaaaa atcacttttt aaaaaagtgt atataaaaag ctataaaata agctaaatag
     2701 gatatttgtc aaatgaagtt ggtgaacaga aagaactgtt ttctttctgt tcattttttt
     2761 attttattag taatgattcc ccatgccaaa tcatgatccg taatttaaag ttataaaagc
     2821 ttcgaaatcc attggcattt cgttggaata ccttgatatg attattcaaa tataaaatat
     2881 agagagaaga gagagaggga agatatttat aaagggcaaa gttatgttta ctcaacttag
     2941 gaaatagaaa tagatcaatt tatattctct tgtcaatcga atatgaacct tagcactata
     3001 taccaaatat agtaaaatgc tgaaagatg
//
LOCUS       DACDIE010000136         2945 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.139, whole genome shotgun sequence.
ACCESSION   DACDIE010000136 DACDIE010000000
VERSION     DACDIE010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.139"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>2945
                     /locus_tag="IV250_002780"
     CDS             <1..>2945
                     /locus_tag="IV250_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YSIRK signal domain/LPXTG anchor domain surface
                     protein"
                     /protein_id="HAP8770007.1"
                     /translation="IGVGLVLGTTELVKAQDEISPSSPLETAISSVQVGDKVASGNTF
                     QENPGYTKNYNFSDLQFSPQELTGDTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDER
                     LAKYVEKIQVDPKKGIGSSRRTFVRINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQ
                     TAPNGVITFEKSLDEIFKEIGIDNLKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTD
                     SDDFYNSLDVSENNPDQFKHGSVSAKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYN
                     FSYSSSAKGTPWYANYKIDERLVPYVAGIQMHMVQADKVTYDVSFESGTKVADLAIER
                     REGHENYGIGSITNNDLTKLIDFANASPRPVVIRYVLQLTKPLDEILEDMKATAQVEE
                     NKPFGEDFIFDSWLSDTNKKLIQNTYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKP
                     ELEEVYDVDTTVKGKVFIHELAGTGHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVE
                     FEFTGVDSSKLIAKDELKIQIVSPGFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKE
                     SIRNNKNLPEDAEYSWKTEPDTSNVTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMEN
                     DATYVPITTTPETTVQSGKPTFDKPDVPLAKDAFSISDVYNKYFGNASVDANTGIVTF
                     TPAKGVGESEPITGTIPIKIVYQDGSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFT
                     AGEGTSIESGTTVFAVKDGVSLPEDKLPVLKAKDGYTDAKWPEEATQPITADDTEFVS
                     SATKLDDIIENPGENIPAGYHKVTFTAGEGTSIESGTTVFAVKDGVSLPEDKLPVLKA
                     KDGYTDAKWPEEATQPITADDTEFVSSATKLDDKSDADKYNPEGQKVTTELNKEPDAF
                     EGIKNKEDLPKDTKYTWKEKVDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDA
                     DKYEPTVEG"
BASE COUNT         1092 a          478 c          619 g          756 t
ORIGIN      
        1 tgattggtgt tggtcttgtc ttgggaacaa ctgaactagt taaagcacaa gatgaaattt
       61 ctccaagcag cccacttgaa acagcgataa gctccgtaca agtaggtgac aaagtagctt
      121 cggggaatac ttttcaggag aatcctggtt atacgaaaaa ttataacttt tcggatttac
      181 aatttagtcc tcaagaacta actggagaca cactaaaagg caatacaatt ggatttgaag
      241 tttacggaaa acataatatt gcagcatcaa ctaaaaactg ggaaatccgt cttcaattag
      301 atgaacgatt agctaaatat gtagaaaaaa ttcaggtcga tccaaagaaa ggaattggat
      361 cttctagacg gacctttgta agaattaatg attcgctggg tagacctaca aatatttgga
      421 aggtcaatta tattcgtgca agtgatggat tatttgctgg ggcagaaaca accgatacgc
      481 aaaccgctcc taatggggta attacgtttg aaaagagcct agatgaaatt tttaaagaga
      541 taggcataga caatctcaag accgatcgtt taatgtatcg aatctacttg gtaagtcatc
      601 aagacgatga taaaattgta ccgggtattg atagtacggg ctacttttta acagattcag
      661 atgatttcta taatagctta gatgtatctg aaaataatcc tgaccagttt aaacatggat
      721 cagtaagtgc aaaatatgag aaacctaata ctcaaacaaa agatggttca ggatccacag
      781 gtgctaatgg agctataatc ttagatcata agttaacgaa aaattataat ttctcatatt
      841 catcttccgc aaagggcacg ccttggtatg ctaattacaa aattgatgaa cgattagttc
      901 catatgtagc gggtatacag atgcacatgg ttcaggctga caaagtaaca tatgatgttt
      961 cgtttgaatc cggaacaaaa gtagctgatt tagcaatcga gcgacgggaa ggtcatgaaa
     1021 attatggcat aggttcaata actaataatg atttaactaa acttatcgat tttgctaatg
     1081 caagtccacg tccagtcgtt attcgatatg ttttacaatt gactaagcca ttagatgaaa
     1141 tcttagaaga tatgaaagca acagcacaag ttgaagaaaa taaaccattt ggtgaagatt
     1201 tcatctttga ttcttggttg tcggatacga ataaaaaatt aatccagaac acttatggaa
     1261 caggttatta ttatttgcaa gatattgatg gtgatggaaa ccctgacgat aaagaagaga
     1321 gcggagacac gaatccatat atcgggaaac ctgaattaga agaagtatat gatgttgaca
     1381 caacagttaa ggggaaagta ttcatccacg agttagcggg aacaggtcac aaagcccaac
     1441 ttgttgataa agaaggtact gtattagcag aaaaaactat cgctccaaat gaaaaagatg
     1501 gggctccaat ttcagatact gtagaatttg aatttacggg tgtagattca agtaaattaa
     1561 tcgcgaaaga tgaattaaaa atccaaatcg tttctccagg ttttgataaa ccagaagaag
     1621 gttcaaccgt tattaaggaa tcaccaaaag cggttgataa acaaaccgtg gtagttggat
     1681 ttaaaccaga tgctaaagaa tcaattcgga ataataaaaa cttacctgaa gatgcagagt
     1741 attcatggaa aacagagcct gatacttcta acgttactga tagtacgaaa ggtattgtaa
     1801 ctgttaagat cggaaatcga actttcgacg tggatgtaga gtttgctgta aaagcttctc
     1861 aagctatgga aaatgatgca acatacgtac ctataacaac aactccagaa acgacagttc
     1921 aaagtggaaa acctacattt gataaaccag atgttccttt agctaaagat gccttttcaa
     1981 tttcagatgt ctataataag tacttcggta atgcaagtgt tgatgcaaac actggtattg
     2041 ttacattcac tccagctaaa ggtgtaggag aatcggagcc gattactgga acaattccta
     2101 ttaaaattgt ttaccaagat ggttctgtag gcacgaccga tttagcagta actgtaagta
     2161 aagatattta tgaaaatcca ggagaaaaca ttcctgcagg ctaccacaaa gtaaccttca
     2221 ccgcagggga aggaacaagt attgaaagtg gaacaacagt ctttgcagtg aaagacggcg
     2281 taagcttgcc agaagataaa cttccagtgt tgaaagcaaa agatggttat acagatgcga
     2341 aatggccaga agaagcaact caaccaatta cagcagatga tacagaattt gtatcaagtg
     2401 caacaaaact ggatgatatc attgaaaatc caggagaaaa cattcctgca ggctaccaca
     2461 aagtaacctt caccgcagga gaaggaacaa gtattgaaag tggaacaaca gtctttgcag
     2521 tgaaagacgg cgtaagctta ccagaagata aacttccagt gttgaaagca aaagatggtt
     2581 atacagatgc gaaatggcca gaggaagcaa ctcaaccaat tacagcagat gatacagaat
     2641 ttgtatcaag tgcaacaaaa ctggatgata aatctgacgc tgacaaatat aatcctgaag
     2701 gtcaaaaagt gactacagaa ttgaataagg aaccagacgc atttgaggga attaagaaca
     2761 aagaagattt accaaaagat actaagtata cttggaaaga aaaagtagat gttagcgcag
     2821 ctggaaataa aaaagggact gttgtagtga catatccaga tggatcatct gatgaagttg
     2881 aagtagatgt tacagtgaca gacaatcgtt ctgacgccga taaatatgag ccaacagtag
     2941 aaggc
//
LOCUS       DACDIE010000137         2816 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.140, whole genome shotgun sequence.
ACCESSION   DACDIE010000137 DACDIE010000000
VERSION     DACDIE010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2816
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.140"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            188..511
                     /locus_tag="IV250_002781"
     CDS             188..511
                     /locus_tag="IV250_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770008.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            675..929
                     /locus_tag="IV250_002782"
     CDS             675..929
                     /locus_tag="IV250_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770009.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            999..1244
                     /locus_tag="IV250_002783"
     CDS             999..1244
                     /locus_tag="IV250_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8770010.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            1320..1685
                     /locus_tag="IV250_002784"
     CDS             1320..1685
                     /locus_tag="IV250_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770011.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(1746..2309)
                     /locus_tag="IV250_002785"
     CDS             complement(1746..2309)
                     /locus_tag="IV250_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770012.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
BASE COUNT          834 a          493 c          432 g         1057 t
ORIGIN      
        1 catcataaat aaaatttatt tttgttactt ttcaactata gcacgaatga caaactctca
       61 caagtttcaa aatataagta aaataatatt gttattctta tttcttttac ttgtgtcata
      121 actattttgt tactttaatt tttatttgct tattttagaa aaaatgttat tcttttattt
      181 attctcaatg ttaggagtga ttattttgcc atttattgcg attttattcg atttagttgc
      241 ggcagcagct tattttctcc aattaaatta tcaaacctca ggaattctgt ttattggaat
      301 gatttttcaa ggtgtgatta cccttttgct tcttgtcatg atgctcagct ataaaggaaa
      361 aagatatgcc cgtattcaaa caaaagtatt tgttaaatat gtttccattc gctatgggat
      421 catcattctt tcctttttag tgaatgcgat cgtcctcttt ttatatgtat tgaattactt
      481 agatatcaat ccattgattt tttctcgata gataagacac attcagcgga acagttcctg
      541 cttatcagca gaaaactgtt ctgctttttc atttttcatt gatctattct tcttttattt
      601 tcctagcatt tcatttgtga tgtatgcaat aacatgttat cttttattat atgaatcgtt
      661 gaggagtccg ttacatgatt acgttggtga gagttttgtt ttgggtgcct gcggtcgcac
      721 tggtcgcaag tattgtttat cttatgaatt ggaacaaaga acggttttat ttagctattt
      781 tgactcttcc ggctatctac tttatgtgga aagtttttaa ttacaactac tttgaacccg
      841 atagtgtttt tatagaagaa ttatcaggac tggttttgtc tttattgatc gtcattcttt
      901 atttgatacg tttaaataaa aaacattgag tactattgat tgcatgtgta gctttctata
      961 gtcattataa ctattctact ataatgaaag gtgattaaat gagtatcgaa gaagctattt
     1021 tactggatac tttacgttgt cgtctgaatt tcttaagaac tggccgatct ttatctcaag
     1081 tagctgtttc tagcgcgtta agtattcctt tattcaaata tcgtaagtac gaagaaggag
     1141 aagaaattcc aagcatcatt gaattaatct atatcgctga cttttacgat atttccgttg
     1201 attacctaat tggtcgctca gaaacagcaa gcaaactgaa gtgatttttt ccagccttgg
     1261 tgctggtttt ttcatttaac gtctaaggtt aacaatctat atataaagga gacatttcta
     1321 tgcgattatg gcatgaaaaa ttgatttcaa agcttcctcg tcaacagctt ttaggacaac
     1381 atcgggaatg ctgtgcatta agaggaaatg gctggggaaa gaaacatgct actgtggatt
     1441 atgtttttca ctattctcct tatagattat tccaatatca tcagcttgtc atggatgaaa
     1501 tggaaaaacg tggttacagc ccagcaccag agtggaaaaa tcctatgtat cgtggcaaat
     1561 catgcgaagc attttttgat ttacctactg tagagcgcaa cgtgccgatt tatccggaac
     1621 acgatgaagt ttatctagca gaatgtttag caaatttgga gcaaaaagga atttatctta
     1681 cctaatataa ctagaaaagc cttctatctc ctcttacttc attaaaaagc tttaaccatc
     1741 ttagatcaag ctaaaatgtc gtaagtttta gggttatctt catcctcacg tgcaaaacta
     1801 aaagcgtact ctagttttgg ttcatcgcca acttccctag acataaatga acatctcctg
     1861 acgtggttta aaagtaccgc atcttcgacg tctgtatacg atgcacagca cagattgctt
     1921 cttttgatct ttcttttgat ttttctgttg cttcttctac accttcttcg attactacat
     1981 cgatgcttac aattgcttct gcctaatatt cccatgcatc gtcgatttaa gcctctgtag
     2041 tcactgtgat cgtttccaca ttttaactcc tctgtcgtgg cttcagttga ttttgtgatc
     2101 agttcagttg ctaagccaga gtcagaacaa cctgccattg ctagcgttaa tattgatagt
     2161 aaaaatagtt tttttatcat aaggttcccc ccctgaaaat agactaaaat aataacgctt
     2221 tctttttttg ttttcagacg aaaattaata atttctaaat attctttaaa aaagcttata
     2281 actattatgc cttttttaat atataacaca gttattttta aaaataagaa tgctaaatca
     2341 ctctacaata acggcgaaag cacaaaaaat aaaaaaatta ttacgttatt aaaaaaacag
     2401 atgaaagatc aggttttatt agatctcatt ctttcatcac ttgaagggat ttaatgaact
     2461 actttttcct ctttgtcgtt tttatatatt cgtattcctt caaacagtag gatagcctct
     2521 ttcaatgctt gatatttgaa ctgtccgtta cttaaaaatt tagatgtgcc gccctcttcc
     2581 tagaacatat tcttttttac tttttcatta attgacagac caatcgaacg aaactgtaaa
     2641 tttcgatagg ataatgattg gtcaaactca ttcattttca agacatctcg attcatattc
     2701 aagataggaa tctttttatc aacatcactt tattcgtatt ttcctctacc ataaaagctt
     2761 cacgctccac aaaattgttt tatacactac tattcagccc attcaacgac caatga
//
LOCUS       DACDIE010000138         2701 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.141, whole genome shotgun sequence.
ACCESSION   DACDIE010000138 DACDIE010000000
VERSION     DACDIE010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.141"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(65..370)
                     /locus_tag="IV250_002786"
     CDS             complement(65..370)
                     /locus_tag="IV250_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770013.1"
                     /translation="MSVDKKATMKRIAELTKSESWQEDKEIVAEVQKLGKSMWAEKTK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEGK"
     gene            complement(367..528)
                     /locus_tag="IV250_002787"
     CDS             complement(367..528)
                     /locus_tag="IV250_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP8770014.1"
                     /translation="MTTEEVIQMRIRNIQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            complement(543..902)
                     /locus_tag="IV250_002788"
     CDS             complement(543..902)
                     /locus_tag="IV250_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HAP8770015.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCHQYQA
                     PLILAKKYRNTFKEERF"
     gene            complement(899..1750)
                     /locus_tag="IV250_002789"
     CDS             complement(899..1750)
                     /locus_tag="IV250_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP8770016.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALEKAKSYTNEVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNTELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            complement(1767..2597)
                     /locus_tag="IV250_002790"
     CDS             complement(1767..2597)
                     /locus_tag="IV250_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP8770017.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKLQSNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
BASE COUNT          639 a          591 c          402 g         1069 t
ORIGIN      
        1 tctttctgaa tcttcatcat gattttcttg aggatgatgt cactgtattg tgtaatgaga
       61 tccattattt cccctccaca tacctaaact gtcgtccttt tgaatcaatc cataagctcc
      121 tagctctatc ccagatgatg tttttgctca gaccagtaat ttcagataac tgttcagcgg
      181 tacctgttac tagaattcga tcaccatgcc agattgcaat ttttctcggc gttttccgtt
      241 tggttttttc agcccacatt gatttaccga gcttttgaac ttctgcaact atttctttgt
      301 cttcttgcca agattctgac ttggttaatt cagcaattcg tttcattgtc gctttcttat
      361 ccacgctcat tcctccaatc tacgaatttc ccttcttaag ttctctatgt gcaaatcgat
      421 tgcctttctc gccgtttcat tgaccatcac tgcctttgtt cgttctagat cgtcaatttc
      481 acgctgaatg tttcgaatac gcatttgaat cacttcttct gttgtcatga tggaccacct
      541 cgttaaaaac gctcttcctt gaacgtattc cgatattttt tagctaatat caacggcgct
      601 tgatattgat gacagaacaa ctttgccttg atcttaaagt cttttgtctg cattcctttg
      661 acatctacga ctttgacaag tttgccgttt ttataaaatg tgaagtcggg aatatactcg
      721 atcttgcgat acttctttcc gtctagttca aatttcggca tcagctcaaa tctttcctga
      781 agttttactt tccagccatt tgcttcagct tgccacaagg ctagatcgta atactctgct
      841 tctgcgatag aatcaaactt gatacctcga tgaacagttt ttttattacg gtatttattc
      901 atgcgatact acctttcact ggttttatgc gcttgtctgc tgtttgttgg aatttcagcg
      961 cataaccttc tgaattctta aatatcctag aaacaattct ttcaccgtag gcttctctta
     1021 gttcaggacc agataagttt gttgtgatga tcgttgcctt gttctgtctg gcttctaaga
     1081 gcgtgtttaa cgtgttgttt gtaaactgcc tactatttga taccccacta cctaattcag
     1141 ctccaatatc gtcaaaaacc accaaatcag ttgttttaat atcggctata agcgatcctt
     1201 caatttcttt tctcagttca gtattgttat aagaaaactt tatttgctct aataattctt
     1261 gatagcttat aaaaagtatt ttcttgtcat aatttgagcg ctcaagtatt tcccaagctg
     1321 tggccattga taagtggctt tttccgcttc ctgatttccc tgatagaatg aaatgtgcag
     1381 gatggttcag cagaacctca ttcgtatagc tttttgcttt ttctaaagcg atttttgttt
     1441 cttgatccac tacgtgataa ttttccattt tgcatttaaa caaagtttta tctgttaata
     1501 ccgaaccatt ttgaaaaaaa ctcaacgctc gtgcttttaa gctatcgtta tatatccgtt
     1561 cggtctgtat atcctctttc acacgtaacg ctttataacc acaactcatg catgttggtt
     1621 tacaacgttc tgaaccatcc ttatttttag ctcgccaact atacaaaggt tcgctacatt
     1681 ctggacattt tccgctttgc actaatactc ttcttattag tttctccata gcatttgcta
     1741 ggctttccat gtgatgcatc tcctttttaa attggcaagt cgtcatattc actaggattg
     1801 ctgtactgta gtttttgact ttgcttttta tgattcttct tgtctgcttt gatttcgaat
     1861 ttgagcttct caaatttttc tctcaatttc ttagcacttc taatatttcc aaaccaaaat
     1921 tcatttgtag gtagccaatt gatcacatac tcaatcgctt ctatagatgc tttatctctt
     1981 tcttccatca acctgattgt gtctgcccat ttttcgatat ctactttgtt catttctttt
     2041 ggaaaatctt cagttaaatt actttgcaac tttttagcaa ggcgtaagtg ttcgtcagaa
     2101 tacttacctt tcttttcttc tttatctata tctttatctt cttctatatc tttatctgta
     2161 ccgtcacgtg acgtcacgct aacgtcattt tccaatttga gacgttcctg tctctttctt
     2221 tccctgtatt tacggtttct ttcagcattt tttagcctta ctttatccat accctcgata
     2281 ttttgatgtt tttcccaatt actgatggca attagtccat cactgcttag atcaatcatg
     2341 ttgaaatttg ccaatgtagt tagcgctaag cgaaccgtat ttacgttttt gccaaacaat
     2401 gtagcaagca tttcttcggt ataaggcatg ttcctctgga tatatatcag accatcgtcg
     2461 ttagtctttc ctgctaagac tagtaatcga atccatataa cgatgatggc atccgactca
     2521 ggaacagctt ggattaatcg tattttttca tcgtcaaaca tagtagtttt aagtttgatc
     2581 caacttatct cagccaaatt tatcctccta tccttaactt tttaattgtt tcctggttta
     2641 acttgatccc tttgatttga tatttatttt tgaaattgat cacacctatt ttgtgtttct
     2701 c
//
LOCUS       DACDIE010000139         2681 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.142, whole genome shotgun sequence.
ACCESSION   DACDIE010000139 DACDIE010000000
VERSION     DACDIE010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.142"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(904..1422)
                     /locus_tag="IV250_002791"
     CDS             complement(904..1422)
                     /locus_tag="IV250_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HAP8770018.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            2540..>2681
                     /locus_tag="IV250_002792"
     CDS             2540..>2681
                     /locus_tag="IV250_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8770019.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQ"
BASE COUNT          890 a          483 c          399 g          909 t
ORIGIN      
        1 gattaataaa aatactaaag ttatttgttc ttttattaaa tacacctaca acttataaat
       61 tgatggtatt tagataacac gaaagaataa aaataacagc tgatagtaaa gtttgttgac
      121 gattgctact caatcgaaag tgagttagtg cgtatgaact tgcttctgaa aacttatgaa
      181 atgaaagacc ttttacatct tgcagaaatc ggtactggga ttaaactaag caataaaaac
      241 taatgttccc ctcattcttt gaacttctac tatttgattt atttattctg ataaatgacg
      301 atttcacctt caggatcttc aaatcccgta tagaatttat ttaagatctc ttcagtatca
      361 gtaataggat aggcgatagg ccacataccc tttgatggtt agaataaact gctcaaaatc
      421 tgtatcgtat gttggcttta gcatattaac aaaggctatc tccaaattat aaaccataag
      481 cgttgaatcg ttcttaacaa tttcaattct tcctaaccca aagtcagaag atataattac
      541 cggacttgcc ttacccttca tgtgggttgt tgagattcca tatttaaagg gaattgtcgg
      601 tactatcggc ggtctgaaaa acatcccaaa aataaaaaaa tctttcccct gctagtctcc
      661 taagtccctt tgtagcggat atggcaccat aatctcattt tatcgaagtg agtcatggtt
      721 tatctgttgg ccaaccaaaa ttgatacaat ctaacgatgc accctctacg gcattctagg
      781 ggagtgcact tgcattcacc caataaaaac cggcagttag caaagaactt atgtagtcgt
      841 atgttcttac tgttaacata caagaggtct cctttccaca attgcttagc agtaaacggg
      901 aatttacaaa gctaatctct tccattttct tatctttgtt ctaaaagtcc caaatggagc
      961 aactgtattt acatgaataa atttatatac ttcccataca gccgttttgg ttgcctcgtc
     1021 tgcccatttt cttttgtgtg cagtgaatag atcttcatca ctcattgaat caatcatcat
     1081 gttaatatca tcaatattac gattgagaat ttcctctaat tctttcaatg gtagatgtgc
     1141 ataggtattt gtaaaccact tatataactc tcctaactga ttccatttaa attgatcaga
     1201 aggcgtttta acagataatc catttctttc atcattttcc cattttagaa ttaaattcgt
     1261 ccaaccaact tgatatgcta aattttcagc aggtgttcta tccacttcct caactctttt
     1321 atccttcaat tcttcaggaa tagtttcaaa ttctaaaata tatttagtat aagtcctatt
     1381 tatttctttt tttaattctt ctttattttt atatgttctc attatattac ctccataaat
     1441 tcctaattgt ctcctttaat tcatctataa tttcatccac gatattttaa tccgttctac
     1501 aatgcatatc tgatatgcat tgactgtcta actcatatgt gaatgattta taaaatatcc
     1561 caacgtctca tctaaagaaa tgttgatcgt cagcaaggag ctaaaaaata cgaatatatt
     1621 tcttttgtag taaaatcgga ctggtttgca tatcattttc ttcgtaaata tcaaaatcat
     1681 gttaactact atcaattact aataaaaact accactttaa gcgttaagaa ctgttaagtt
     1741 tagttaagtg aggatagttc acaaaatcca ttgttaaact tcacgaaagt ggtaaaactc
     1801 aaaatgcttt atataggatt acagcatcgc tctttcttcg attgcgcgtt gggtaaaaaa
     1861 tattatgaag taaaaattga cgatgacacg ttccttacgg ctcatcagat gaaacagcta
     1921 caaaaacgaa atgatcaact cgaagaaaat ttaattctaa aaaagcgatt gccatattca
     1981 cgcctcgctc gacgaactat taaaggtcaa gatttacctg aagatcatcc tttttaggaa
     2041 attgaagctt tttatgttgc acaatgcatt tataatacta caatgatgtt ttcaccagat
     2101 attcttctag ggtgcggtgt aatgaaacag atacacttgc aaaagaaaat tcaccgcgaa
     2161 tttactaaaa ttatgaataa ttatgttaat cttccgaaat ttttttccaa aattaaggga
     2221 tcaagctggt gttatagatt gtttaaggtt ggcaaaaaaa taacttcttt ttaaacaatt
     2281 atagaaaacc gatactctgt taagttggtt gacacggtgt cgatttttat gactttctta
     2341 ttctgaaact tcactaacgg acagtaccta aaaagtctat agtatggacg tttacgagta
     2401 ctattttatc aaaaatgctg ctcatttcaa cttatatgtt ttagtgaact acccccactt
     2461 agcttggata ggctacaatt aactagacag aaaaattaag gtgtgtagac tagaagaaaa
     2521 cataccagga ggaattttta tgtctaagag aacacgaaga actttttcac aagaattcaa
     2581 gcaacaaatc gtcaatcttt acttagctgg aaagccacgt gtagaaatca ttcgagaata
     2641 tgaactaacg gcttcagcat ttgacaaatg ggtaaagcaa t
//
LOCUS       DACDIE010000140         2675 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.143, whole genome shotgun sequence.
ACCESSION   DACDIE010000140 DACDIE010000000
VERSION     DACDIE010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.143"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            81..1166
                     /gene="xerS"
                     /locus_tag="IV250_002793"
     CDS             81..1166
                     /gene="xerS"
                     /locus_tag="IV250_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HAP8770020.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            complement(1450..2234)
                     /locus_tag="IV250_002794"
                     /pseudo
     CDS             complement(1450..2234)
                     /locus_tag="IV250_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922412.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(2269..2418)
                     /locus_tag="IV250_002795"
     CDS             complement(2269..2418)
                     /locus_tag="IV250_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAP8770021.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            complement(2411..2617)
                     /locus_tag="IV250_002796"
     CDS             complement(2411..2617)
                     /locus_tag="IV250_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP8770022.1"
                     /translation="MVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLGL
                     VPVESVEEVLGMKIEVEEDFQNHG"
BASE COUNT          921 a          473 c          376 g          905 t
ORIGIN      
        1 aattgacaaa acattctata attggtatag tccaattata gagatactct tctcccctac
       61 ttattgaaag gatgaatgac atgaaaaata cagaaatata tcaaaaaata gatacaatca
      121 tacttcatat gcccgattat ataaaagatt ttgttcaaga tcgtgaggat aaagatcaat
      181 cacctcgaac cactttagaa tatctaaaaa attataagct cttttatgaa tggcttctga
      241 gtgagtctat cgttcctgat acttctatca cctcgataag acagttaact gcctatgacc
      301 taaacttgta caaatctcat ttgaaacgtc gcacaaaaga aaatgtaaaa aaagagacaa
      361 caaaaactaa gctcaaagat aataataact taggtctatc tacttctact atcaatagaa
      421 atatcacggc attaaaggtt ctatttaaat atctttcgaa aagttccaat aatcctctag
      481 gaaaaccata cttagacaat aatcctatgg atcaagtagc aaccatcaca gataaaacta
      541 cgttagctgc acgtgccaat gccattgaga aaaaattatt tctagataag gatacacaaa
      601 attatctaga ttatattgcc aatgaatata aaaatacttt gagtaaacga gctttgatct
      661 attatcaacg agatgtagaa agagacctgg cgatcaatgc actgatttta ggatctggat
      721 tgagactctc cgaggttata aatattaatt tagatgacct atctttagat aagaataatg
      781 tggtagtaac tagaaaagga aataaaagag atgctgtcaa tatagcagca ttcgcaatgg
      841 aatatttagc taattatcta gctattcgga aagaacgata taaagtaaca gaaaacgaaa
      901 aagctttatt tctagcaatt taccaaggag aagccaagcg tatatccggt attgcaatcg
      961 aacggatggt agcaaaatat tcaaaaggct ttagaattca agtcagccct cacaaactgc
     1021 gtcatacatt agctactaga ctctaccaac agacaaattc attggtatta actgcccaac
     1081 aattgggaca ttcaagtacc aatacaacaa ctttatatac ccatatagac aatgcggcta
     1141 cgatcgatgc attaaatgct ttatgagtat aatgtataaa taggcggaaa taagaaacaa
     1201 cacgctagaa aagcctgtat tatagttttt ctagtgtgtt tcttttggtg tgatcctact
     1261 tttgtttatc atttattttt tattatcgta aataggtgtc ggtttgattt tagaaaaatt
     1321 agtttggata taatctgtgg gatcatttgt tatctctctc aatgaattta gatacaaatg
     1381 accataagtt cctaaagtcg tttgtacatc agcatgtttt aacctttctt taattcgaag
     1441 tgcatctttt tattgtgata taagcaaaga agcatgacta tgccttaatc cgtgtatgcg
     1501 gattatatga acaccaacga tcgttgcatg tcttctcata atatgacgga ttgtatattt
     1561 ctgagtaggt gttccattat aagaaagtac aaacgacatg cctcctattg aaacttgacg
     1621 ctccttccaa gcttttaaaa aggtaattgt attgtcgtct agttcgattg tacgaatact
     1681 agaagccgtt tcgggttcta caaactcata ggttttggct ctcttataat agagtgtttt
     1741 attcacacgc aagtttttat tttcaaaatc aatatctttc caagttaatg cagtggcttc
     1801 tcccaaacgc ataacggtca taaaaagtaa ccacatacaa ataaaaccaa aatgttggta
     1861 caaatcatca atataaaaag ttgaaataac ttttgtaaat tcttcttttg tccaaaaatc
     1921 aactgtagtt ttccttttct tcacatttcc aataactttt gcaggatttt tttcaagtag
     1981 tcccaaaaca actgctcgat ctaatgacat ctgaaaaatc ccatacatgt ttcttacata
     2041 agaattttta tatttgctca ataaattatt ttgccacttt tgaatgtcaa ttggacgtat
     2101 ttctcttaac tccattttaa agaaataaga aaagtttttt tgattgtaga taaccgattt
     2161 ttatatgttc tttcttctac tttcccttga taataaggta ggaataattt ataaaaaaac
     2221 tcctcaaaca acatagtact ttgagaagtt tctgcttctt ttccataatt agaaacaagt
     2281 tcggtatacg ctttttttct tctttaactg ttttaacccc tctacgtttt tttgtactct
     2341 tttccctgtt cgtttgtcga atcccaaact agcagtaaaa tgatatttcc catttttatc
     2401 ttttttaaaa ttaaccatga ttctgaaaat cctcctctac ctctatcttc atacctaaaa
     2461 cttcttctac agattcaaca ggtactagtc ctaatcttct attttcgtaa taggtatagc
     2521 tttttgaaac cataattgct tttgcctgtc gtatgattct ctcagactga tacttcccat
     2581 aacctaattt cattaattca ttttttgtta taaccacctt ttaaccacca cattttttat
     2641 tcaaatataa aaaagaacac tactaaaaat agtag
//
LOCUS       DACDIE010000141         2530 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.144, whole genome shotgun sequence.
ACCESSION   DACDIE010000141 DACDIE010000000
VERSION     DACDIE010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.144"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            202..597
                     /locus_tag="IV250_002797"
     CDS             202..597
                     /locus_tag="IV250_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770023.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            922..1539
                     /locus_tag="IV250_002798"
     CDS             922..1539
                     /locus_tag="IV250_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8770024.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            1580..1879
                     /locus_tag="IV250_002799"
     CDS             1580..1879
                     /locus_tag="IV250_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770025.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            1913..2080
                     /locus_tag="IV250_002800"
     CDS             1913..2080
                     /locus_tag="IV250_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8770026.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(2125..>2530)
                     /locus_tag="IV250_002801"
     CDS             complement(2125..>2530)
                     /locus_tag="IV250_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8770027.1"
                     /translation="GRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLDTAMFD
                     MFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLVFKTVV
                     DMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT          894 a          366 c          475 g          795 t
ORIGIN      
        1 ttaataattc acttgggaca gattttactt atcttgattt tattggagat gaggggaaag
       61 ttgcaaatgg attttctgaa aaaattattc aatttcaaga aaatagaagt aaacttaatt
      121 aagaatcgtt tactatttaa acagtgtcct tttccttttt aaatatatac gataaaataa
      181 gtatatgaat tgtaaaagga catgatgatt atggaagaaa tagaatggaa taatctgact
      241 caaaataata aaaataattc tggatgtgga attatcttta tgtttgttat agttttggga
      301 atagtagtac ttttaataac tgtcaagata gtagattact ttgcgaatac tcctgaagaa
      361 caaatacaga gaaaacttcc tgagtttgaa agagtaccac cttatgatcc aagagataat
      421 tcatttcaag taaaaaaatt aaaagagtct ccaaatgaaa tgtggtttga agttacagtt
      481 gaatcaagcg acttcaaaga aaaaaagaaa gaatacaatt atattgtaaa ggcaaaagta
      541 tcaaaaagat ttatttttaa ccctaaatta gaaatatata gtgttgtaat agaataatag
      601 tgttaaaaaa attgtaaaag ttaaagaagt caaattctga tgtgaaaaat aaaatatacc
      661 atggaagaca taaaagaaaa actggataac tagtttattt aatggcagac cgttaaggcc
      721 tgccattttt atgtatttta gcggaataaa tataatcatg cgggagtgta aacttactgt
      781 ttcaaaaagt atactttttg acatgccttg attttctttg tttatgtatg gcataataac
      841 cacataatac aatttgaaac gtttctgatt taatctgatt gttttgaaac aattttagtg
      901 aaaaaagggt agataaaaac gatgagaaaa tttttttata tccgggtcag tagttatgat
      961 caatcgatcg aacgacaact gatttccgcg aaggaattag gaatccctga agaatatata
     1021 tttgtagaaa aagccagcgg caaagatttt aaacgccctg aatatcaatt aatgaaacgg
     1081 atgtttcgaa aaggagattt actctatcta caatcattag atagactagg cagaaataaa
     1141 caaatgattt tagaagaatg gcgagaatta actaaggaaa agcagattga tatttctgtg
     1201 ttagacatgc ctctgttaga tactgctagt tacaagcaac tagatgggtt agagacgctt
     1261 atctctgact taattctgca attactcagc tatatggccg aagacgagcg gaaacgaatt
     1321 aatagccggc aaagagaagg cgtagaagct gcgaaacaaa agggtgtaaa attcggcaga
     1381 aagaaaattg ctttacctcc tgaatttcct gcgatctatt ctgactggaa agcaggaaag
     1441 ataactgcag tctatgctat ggatcaattg ggcatgaaac gaaatacctt ttatcgacgg
     1501 gtgaaagaat atgaacatac cttgcaccac ttttcttgat agatataaca gctattagac
     1561 gggaagggag agtttgaaaa tggacttaac gaatcaaagg gtcgttttta ctggaacgct
     1621 gcagcaaatg acacgaaccc aagccatcgg tttggttcac tctttaaacg gccattgtca
     1681 atcaagtgtt acttcaaaaa caaatttcct ggtttgtggt cagtattcta agtcgttgtt
     1741 tgatgattct ctctatacaa aaaaggaaca aaccgcgcgg gacttgattg cgttaggtca
     1801 tgagattacc attttatcag aagtcgaatt ttatgcgtta attagtcaac agttgaaaga
     1861 atggcaacgg tatttataat cgttagtaaa atttacaagg aggaaaattt ttatgaagcg
     1921 aaaatcttat gatcgttggg gcattgaaat aactagtttt aatcgaaata ttgcttttca
     1981 cgtggagtgt gactgtgggg aattagcaaa aagtgtttat caaaaaaatt acttttactg
     2041 tcctaaatgc catcgccgat acggggtaca tggaacatag aaacgaaata gtgcggtaga
     2101 aaagcgaatg aatgtagtat atttctacaa atcattcgct ttctttattt tataaatagt
     2161 tggtcgagag atcccgtttt cttttgcaat ctctgcaact ggaatgcctt gctttaacat
     2221 gtccaccacg gttttaaaaa ctaagcgttt ttgcggatct gttgagttaa tagaatactc
     2281 gattggtcgt cccttatatc ggccggcttt ttgagccaat aatactcctt gccgctgacg
     2341 ttcacgaatt ttctcacgct cgttttgtgc aatataactt agcagtgata aaaacatatc
     2401 gaacatagcg gtatccagga gttcatttcc agtattaaaa tctaaaaact gtgcatctaa
     2461 tatttttaac gctacttttt tctgtttcat aaaagcaact gtatttttta tatcttcata
     2521 atctcttcct
//
LOCUS       DACDIE010000142         2507 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.145, whole genome shotgun sequence.
ACCESSION   DACDIE010000142 DACDIE010000000
VERSION     DACDIE010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.145"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..430)
                     /locus_tag="IV250_002802"
     CDS             complement(<1..430)
                     /locus_tag="IV250_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP8770028.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
     gene            774..1181
                     /locus_tag="IV250_002803"
     CDS             774..1181
                     /locus_tag="IV250_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770029.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            1195..1596
                     /locus_tag="IV250_002804"
     CDS             1195..1596
                     /locus_tag="IV250_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770030.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1598..1969
                     /locus_tag="IV250_002805"
     CDS             1598..1969
                     /locus_tag="IV250_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770031.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(2005..2307)
                     /locus_tag="IV250_002806"
     CDS             complement(2005..2307)
                     /locus_tag="IV250_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770032.1"
                     /translation="MLMEGKTQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINL
                     YYSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
BASE COUNT          901 a          366 c          401 g          839 t
ORIGIN      
        1 ctgctcttaa acgaataact tcttcttcaa gagactcatc tttaagtttt ttaggaatgt
       61 ttagcttgga attcatacta atttttctgc cccttttttg gctctcaagt gaagaagcac
      121 cgccttcttc atattgctct atccatttac ttagagttct atttgaaccg atattaaatt
      181 ttttagcagt ttcttggata gaaagaccat ttgtttccat atattctata acatcaagtt
      241 taaattttgt agtgtagctt ttgccacctc caaccaagcc ttcccaacca tgatagttat
      301 aaatccttac ccaatgtcta accagtgtac gatttatttg atatttatgt gcaagatatt
      361 tgtagccgcc ttcgttattt aaatagtctg aaactacttt tttcttaaaa acaaatgtat
      421 atttccgcaa aaaaagcacc ccttttaatt agatttctag tctaactttt ggggtgcaca
      481 tcatttttga ggggaagtac atatagttgt tgatatagtt gctaatattg ttagctaatt
      541 tgttagtaaa actgtatacg aatttagata gtaaaaaaac agtttgttcc ttatatagag
      601 gcacttttac tactgattaa atgtcagata tgcttacaaa tatacttata aatatgctta
      661 caaaattagc taatatttcc gttgacaaag ctttgttact attgttaaac taaattgact
      721 agcaaaagtg aaacataaat ttattaaata aatgtaaaaa ttaggagaaa tcaatgtctt
      781 atagagtaca attaataatt tcagaagatg tagaaagtca gcaatttggc actaatgtta
      841 ttaataaagt aattaatcca gctctttcta ttaatgcgcc tttaattcct actgctttat
      901 cattttctgt aacagctgtg gtgtctgaga tagaggatac agaaaagata aaaatagtag
      961 aaattgaagt tttaaacaag aatgaaaaac aaattttttc tacgggtgaa gtatcggtaa
     1021 gcttgcctcc tcaagttaac gatataaact ttaatataaa cgccagaaat gtcttggttg
     1081 aagaagcagg agagcattat gctgttgtta aatttaatgg aactgagatt ggaaggcaaa
     1141 tctttgatat caaggtcaac aaaccagtgg aaaaaaatta aggagacgat acggatgctt
     1201 gatatagtac catcaaatac acatcggagt ggtagcttaa taaattttgc tgcgattttt
     1261 gcttgcgcat cggtaattct agcatctcca gttgttgtgg atgcaagttc tactccgaaa
     1321 actaaaaatg ataatcaact tgtaattact acaaaaaatg agattaacac aatttcaagt
     1381 aatgatggca aaatttttga tgtgataaat acggttttaa aaagaaaagc tgaaatcaat
     1441 ataaaatatg atgaagattt aaacttgtat ttctttgtta tcaaaacaac atctgaattg
     1501 ttttcctcag attacgatgt tttggatact ctcgataatg tattatctga ctataaatat
     1561 atggggaaat ctgtagtagc aactttgggg gaataaaatg tttgacttta atgaatattt
     1621 atctatttcc aaacaaattg aatcagatga taaatataat tctaaagaat cttatagaag
     1681 aactgctgtt tcaagagcat actacagtgc ttttaaaaaa tctgatgagt atttgaaaga
     1741 aaattatgat attatttata atggaagtag cggaaaagga agccaccaaa ccgtttggaa
     1801 tttgttttca acagttaagg aattgaatac cttaggaata cataatagtg ggtatagaat
     1861 gttagagaaa agaaaatgtg ctgactatat tcctaatgaa accattacga aaacagatat
     1921 ggcgctgatg aatcgagaag cagaaaaaat aataaacaaa ctcacctaat gaatttcaag
     1981 cgagtttgtt tgttattttt ttatttaatc tttgtatctt tctggatcaa cgaaagtata
     2041 ctttatatag tcataacgcc gatgatcgct acgtgcgtcc ggcacgtcag tcacgatatc
     2101 aaacaaaaag tatacaccct tcttcattct agttttcgca gcaggaattt tgaaatagtt
     2161 cttattagaa tagtagagat tgattaataa gctatcttcg attgctaaaa agaaaacttc
     2221 tgaattccat actttataaa aatctttgat aaatctattc gaagggtcaa atttaaacca
     2281 taattgtgtt tttccttcca tcagcataaa agttcacctc aaaaagagta tacgaacgaa
     2341 tgttcttttt gtgaaggcgg aatttaatat actgaataat agaaaacaag tacttgtgcc
     2401 aaattatgtg ccaaaaaaat tcgaattcaa caaaatgtaa acaaatagaa aaccttctat
     2461 attaagattt cctgcaataa caaacacaac agaagacgtg taatagt
//
LOCUS       DACDIE010000143         2497 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.146, whole genome shotgun sequence.
ACCESSION   DACDIE010000143 DACDIE010000000
VERSION     DACDIE010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.146"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(526..1866)
                     /gene="glnA"
                     /locus_tag="IV250_002807"
     CDS             complement(526..1866)
                     /gene="glnA"
                     /locus_tag="IV250_002807"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HAP8770033.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(1894..2286)
                     /locus_tag="IV250_002808"
     CDS             complement(1894..2286)
                     /locus_tag="IV250_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP8770034.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT          720 a          550 c          418 g          809 t
ORIGIN      
        1 tcgtgcctcc ttatttctaa ataaacggtg ttctcctgcc aactcctcgg tcacaagtca
       61 caatatccat caaacatgag aagctgtttt tgaaattatt tcttgtgatt cggagttaaa
      121 tggtaggctc acaacctctg gataaacggt attcaaaagc cagtttctcg gtattaagcc
      181 aaatttatgc aaaaatgtag aaagccattt tcacaaaatt tttcttaata ctcgaaacta
      241 aacgtctttt tcaaaacctc ttggtaaacg gtgttcaaaa gcttgcatct gcggtaataa
      301 gttcagccaa ttcaaaaaca agaaaagctg tttttgcttg gctattctta ttacttgttg
      361 cagggcagct ttttcacaac ctctccacta aacggtgttc aatcagctga ccctcggtat
      421 tagctcaaaa aacggcaaaa tatgaggaac tatttttccg ttagtttctc taatactcag
      481 gtcaaaacgc tgatttcaca gcctctaatt cacggtattc ctgttttaat aaagttctag
      541 atattgttct ctttcccact cggaaacagt ttgtctgaat gccgcccatt cgagacgttt
      601 tgcttcaaca aaatttgaga agatatgttg tcctaatgcg tcaatcatta cattatcctt
      661 acgcaattct ttgatagcat tatggatagt tgaaggtaag tcctggatat gtgcagcttc
      721 tctttcttcc tcatccatta cataaatgtt acggtcaaca gctgctggcg gtgtgatctg
      781 attacggata ccgtctagtc ctgcttgcaa caatactgcc attgccaagt atgggtttgc
      841 agatggatca actgaccgca attccaaacg tgtggataaa ccgcgagatt ctggtacacg
      901 tactaatggc gaacgattac ggccactcca tgcgacataa acaggcgctt cataacctgg
      961 aacgagacgt ttataagaat tgactgttgg attgcaaacg gctgtgtagg cacgagcatg
     1021 tttcagcaaa ccacctaaga aatgataagc tgtttcactt agttccaatt caccgtcttt
     1081 atcatagaaa acatttcctt catcattgaa caaagacata ttgcaatgca taccagaacc
     1141 attgatccca aataacggtt tcggcatgaa agtcgcgtga agtccatgct tgcgtgcaat
     1201 cgttttgaca acgagcttga aggtttggat attatcacaa gcatctacca catctgcata
     1261 tttaaagtca atttcatgct gtcctggcgc tacttcatga tgtgaagctt ccacttcaaa
     1321 gcctagactt tctaattcca acacgatgtc acggcggcaa ttttcaccta aatctgttgg
     1381 tgcaaaatca aaataaccac ctttatcatt caaggtcgtt gtgatcccac cgtcttcgtc
     1441 taatttaaat aagaagaatt ctggttcagg tcctaaattg aaagaggtaa aaccaaggtc
     1501 gcgcatatct ttcaatgctc gtttcaaatt cccgcgagga tcgccggcaa atggtgtccc
     1561 atccggatta tagatatcac aaattaaacg agcaactttt ccatgagcac tttcccacgg
     1621 gaagatcatc caagtggaga gatctggata caaatacata tcgctttctt caattcgaac
     1681 aaatccttca atcgaagaac cgtcaaacat cattttattt tctaatactt tgtcgatctg
     1741 gctcactgga acttctacat ttttgatcgt acctaagatg tctgtgaaca tcaatcgtaa
     1801 aaatcgtacg ttttcttcct caatgattcg tttgatttct tcacctgtta tctgtttctt
     1861 taccatggta tccctcttcc ttttttaatt tattcagtca aagaattgtc tttgcaatcc
     1921 ttgcgactga taatgacctg ttttgcttaa cccaccttgc gccaaaatct catcacgaaa
     1981 aatttgtcga acgtcatgat ctgtcaacgg cttcttgttt tttgcttctt gttttttcgc
     2041 ctgtgccatt tcgtatacac gtttgatccc agccatattc aatccctctg aaagatagtc
     2101 tttaatctca agcaacatat caatatcatt cagcgaatac atacgtcggt ttccttcgct
     2161 tcgttccgga tgaactaaat cttgttcttc ataatagcga atttgccgag cagacaaatc
     2221 agtcagcttc ataactgtac caattggaaa tacggacata gatcggcgca attccttttc
     2281 tcccatcaga cgtccccttt cgttttataa gttggctaaa gtgtaccaat taaaaaaaat
     2341 attgtcaact gcatatgtta ggttttctaa catgagatct ctatttttta taaaataaca
     2401 aaaaaataag aacacaaaaa aggtatctag ttgattttcg taccagatac cttttatttt
     2461 ttattatact gtactatcct ttattagtgt ttttact
//
LOCUS       DACDIE010000144         2473 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.147, whole genome shotgun sequence.
ACCESSION   DACDIE010000144 DACDIE010000000
VERSION     DACDIE010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.147"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            5..1149
                     /locus_tag="IV250_002809"
                     /pseudo
     CDS             5..1149
                     /locus_tag="IV250_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
     gene            1320..1778
                     /locus_tag="IV250_002810"
     CDS             1320..1778
                     /locus_tag="IV250_002810"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAP8770035.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1937..2407
                     /locus_tag="IV250_002811"
     CDS             1937..2407
                     /locus_tag="IV250_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HAP8770036.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          824 a          465 c          465 g          719 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgtcagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagtgac ttctaaccaa aagatctagc cgatcagatc
      661 ggattttacc aaaatgctat cggagccgta cttggtcccc aagatagctg gttacttcag
      721 cgcggaatca agacactaag tgttcgtatg gaggaacatc aaaaaaatgc ttttgtggta
      781 gctgattttc tattttctca tccagcagta gaaaaggtct actatccagg attacccgat
      841 catgaactcc atggagttgc taagcagcag atgagcggct ttagcggaat gatttctttt
      901 actttaaaaa atgaagaatc tgctattcca tttgttgaat cactccaact atttacttta
      961 gcagaaagtt taggtggcgt agaaagttta gtggaaattc cttctgtcat gactcatgcg
     1021 tctattccaa aagaaaaacg agaagaggct ggcataaagg acggcttgat ccgtctttct
     1081 gtaggaattg agtatggtca ggatctcata aacgatttag ctcaggcatt tgacagaatc
     1141 aagaactaga agtcccaata aacgacttag cagaaaaatc taaaaaatat ttcagattgc
     1201 tacaccttgg aaacgataag tgttagaatc aagtagaaag acgtttttgt aaatttcact
     1261 aaataattct ttagtgaaaa gcatcaaaac gaatgaacaa acagatagga gtcttacaaa
     1321 tgagaatgaa tgtcgaaagt tttaatcttg accatacaaa agtcaaagca ccttatgtac
     1381 gtgtagctga tcgaaaaacg ggtgcgcatg gcgatgtcat cattaaatac gacgtccgct
     1441 ttaaacaacc aaataaagaa cacatggaca tgccaagtct acattcactt gagcacttga
     1501 ctgccgaact aatccgcaat cacgcagatt acatcgtaga ctggtctcca atgggatgcc
     1561 aaacaggctt ttacttgact gtattgaatc atgaaagcta cgaagacatt ttagatgttc
     1621 ttgaagcaac aatgaaagat gttcttgaag ctactgaagt acctgcaagc aatgaaacac
     1681 aatgtggctg ggctgccagc catacactag aaggtgcaca agctttggct cgtgaatttt
     1741 tagcaaaacg cgatgaatgg tctgaagtag aagcatagtt tctaaaagaa cgtaaagatg
     1801 agaagcaata tagctatctt atcttcgtat tttattaaat atttattgtc tgaaaagagt
     1861 tctaaacggg aaaacaaata gctagttttc agtttagcgc tctttttatt atataaaata
     1921 gaaaggagaa aactttatgt ctatttataa ttttgaaact actttagaag acggcactgt
     1981 ctattcattg aaaaaatatg caggcaaacc gataatcatt gttaacactg caacaaaatg
     2041 cggtctagca cctcaattta aacaattaga agaactttac caagcttatc agacacaagg
     2101 tctggttatc ttaggctttc cttccaatca atttaaacaa gagctagatt cagcgactga
     2161 agcagcagaa gcttgtaaaa ccacttatgg cgttaccttc cctatgcatc aattgattga
     2221 cgtaaatgga gaaaatacag atcctctttt tacttatctt aaaaatgaag ctcctggtac
     2281 tgtcactaaa gcaataaaat ggaattttac taaatttttg attgatagaa atggcaaagt
     2341 aatcgaacga tttgcaccac aaacttctcc attgaaaatg accgctgcaa ttgaatccgt
     2401 tctttaactt aataaaaata aaaaaagatg aactatttct agttcatctt tttgcctttg
     2461 cgtggcgacg tcc
//
LOCUS       DACDIE010000145         2462 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.149, whole genome shotgun sequence.
ACCESSION   DACDIE010000145 DACDIE010000000
VERSION     DACDIE010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.149"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(85..1317)
                     /locus_tag="IV250_002812"
                     /pseudo
     CDS             complement(85..1317)
                     /locus_tag="IV250_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            1480..2292
                     /locus_tag="IV250_002813"
     CDS             1480..2292
                     /locus_tag="IV250_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8770037.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT          743 a          517 c          419 g          783 t
ORIGIN      
        1 ttctcattcg atggttgtgt tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg
       61 ttgtgtaagc agtactttgc ttttttagta gcttgttttc tcatttgata tactttgtaa
      121 ttttcgttca taatactcaa cctcctcctg ataatactcc gggtctgcct tcatattttg
      181 gagaagtttg atctgtcttg gattcatttc tccttgttca ataatcaaag gcaacaagga
      241 agaatgaaac gtttgatcga cctgtgcaat aaaaactaat gatggtgaaa caccatgaat
      301 taaccaactc atagttttct caaaactttg aggaattgta tctaaagaaa tggtgattgc
      361 gccataattg ctaataaagg ctgcccattt ggcatcaact tttgcaccta gatcatttgg
      421 atcacggtca taaaaacaaa ttgctccatt aagtaactca aagaacagtt tggttaaatc
      481 atgggttttt gaaataatct ttgccaagct ttgcgctttc tcttttcgat accgtagttc
      541 aaagcgattc tttacttttg cctcttctaa aggaattcct cgtcgcttcc gttgttcatg
      601 gtccttctga taaaagcaaa aacgacactg actcttcttt gagcctaaat acaaggtctc
      661 accatttgat tcattgttta tgatatccgt accgccatga gattcactca cttgaaaagt
      721 ggtccacaca tggcctagta ttaccttatg cttaagttct ggaatgctga gactccccac
      781 aaaatcatct aaagtaaaat caatccgtgt aaagttcccc tgataatcta ataccttacg
      841 aaaaaactgt tgccaatttg tttttcgtgt ttttagccat cttgctaagt gtttacagcc
      901 ttgacctgaa aactccatca cagttccttt aaccgcatcg tcaatggaat accgaatcgt
      961 gattacttga ttccacacgt attggccaat atagcccttt ggcgcataat ccagttcctc
     1021 aaaacgttca atgggcattt caataatctc ttcaattagc tttaaatgtt gattcgagaa
     1081 aaagtgaatg gttagataat caatcaaaac acggttaact ggaacgacct cttcttgcaa
     1141 actatccaac acaagattca atttttcttc acttaattgt ctcgttcctt tttctacttt
     1201 cgataataac tatctcgaaa tccccacttt ttctgctaaa tatcgttgtg tccaccccct
     1261 ttcttctcga gaaactcgaa tggcttcacc gagatcatgt tgattaactt ggttcatcga
     1321 caaacctcct tctgctattt acttgtcaaa gaacctattg aacttatttt aagtacattt
     1381 ttattgtacg cctgtattta ttttaagtca atatgttttg acttttttta agtacacacg
     1441 tatactaaat ttaagaactt atcctgagga ggagttttca tggagataga caagcaagca
     1501 gtcggcaatc gaattcggca gattcgccaa gaattaaagt tgagtatgga gaaatttggc
     1561 aaactaattg gtgatttacc gcgaagtaca gtcaataact gggaacgtgg gatcaacctt
     1621 ccaaaaaccg aaacccttca ccaaatcgca gagataggtc atgtcaccaa tgaatatctt
     1681 ttgtatggtg atcaagagaa tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag
     1741 cttgatccta agattgaagg tctcatagta gacgaaatca aacacgctga ccttgttagt
     1801 gaaaaagaaa tggatcgaat gattgaattt tttattacaa atctcattcc accaactgaa
     1861 caagaccagt ttactttcca atgtatcgat gaaaaaaagc agctttatct tggttcaact
     1921 aattttggta aggaagccca gctctatttg caacatgata atcaaaatca tattctgcac
     1981 atgatgccct ttaccttctc caactttagt gtggatcgca tactcgtcta tttagcaaat
     2041 caaaaatcct attcctattt tgggaaacat ttacccaagg aactaggtga gaaagccatc
     2101 cttctatact caattaatca attgaccagc gatgtgcgaa ttgccccgtt ggtctattca
     2161 gaagaaaccg cttcttatca gtacacagag gacaatcaat atctactaga acagcaatat
     2221 ctttatctac cattcgtgat ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca
     2281 tcatcaaaat gaggtgacaa aattgataag cacgtgacaa agaaaatact gctaggacac
     2341 tccccaatgg gtagttgtca cagtttcctg ttctttgtta gccccctgtt agaaccgggg
     2401 gctttattcc tgcagtccgg ctacgccttc ctgcaataga caaaactcca gatcgggtaa
     2461 tt
//
LOCUS       DACDIE010000146         2352 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.150, whole genome shotgun sequence.
ACCESSION   DACDIE010000146 DACDIE010000000
VERSION     DACDIE010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.150"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..544
                     /locus_tag="IV250_002814"
     CDS             <1..544
                     /locus_tag="IV250_002814"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP8770038.1"
                     /translation="ISKENLLNQDFSTETICEKWAADITYIPTKKNGWCYLSSIMDLH
                     TKRIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWLKTNK
                     IRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNRNRIH
                     SSIHYLTPQEFEELAKEKMA"
     gene            complement(1017..1589)
                     /locus_tag="IV250_002815"
     CDS             complement(1017..1589)
                     /locus_tag="IV250_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8770039.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENCVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1605..2210)
                     /locus_tag="IV250_002816"
     CDS             complement(1605..2210)
                     /locus_tag="IV250_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HAP8770040.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          741 a          468 c          359 g          784 t
ORIGIN      
        1 catttcaaaa gagaatctct taaatcagga cttttctact gaaacgatct gtgagaaatg
       61 ggcagctgac attacgtaca ttcctactaa gaaaaatggt tggtgttact tatcttcaat
      121 catggattta cacacgaaaa gaattattag ttatacattt tcaaaacgaa tgactgtgga
      181 ttgtgtcatt caaacattga ataaagcaaa aatacattat cacattccag aaggaatgat
      241 tctacacact gacttgggca gccaatacac agcaagagaa gtggaacaat ggcttaaaac
      301 caacaaaata aggcattctt atagtagaaa aggaacacct tatgataatg ctggaatcga
      361 atctttccat gcctcattga aaaaagaaga agtctacacg actagctact cagattttga
      421 agaagcaaat cgagcgctat ttagctacat tgaaggattt tataaccgaa atcgaatcca
      481 tagctcgatt cactatctaa ccccacagga atttgaagaa ttagcaaaag aaaaaatggc
      541 ttaaccgtct ctactaaaat gtgtccaaga tattgactca aatccagtaa tatcacaata
      601 ggaatgctgt ttattatatt agcagcagtg ctgattttta tgatagttat ttggttctta
      661 aatgaaagat ctaaagacaa tcaacaccgt agggatatgg agataaaatc aatagagtca
      721 atagattatt tatcgataat aacactaaaa aagccacgtt cctaaacgtg acttacgctg
      781 aacgatatgt gttgtttggt agacgaacaa atcgttcatt ataaatatat gatggtatcc
      841 taatatagta agcgtggcta gcataccttt aattacttta ccttttacag ttaccataca
      901 cattttttga gcagaaatca agtaaataaa aaaatattat atttcacaac atgctaatag
      961 actcatatct agaacgcatt aagtatttac ctgtctggtt cgctgagact tcttttttaa
     1021 ctttcttcaa ttttcttctt tattctaaaa agcgtactgc gactgaaacc ggttatagct
     1081 tcaacttcct tatacgattt acctgaagta agcagttcat atgcgtgcct cttcttcggt
     1141 gtgattgttg ctttcggacg tccttctctg aaattaggat tattctttct tgcaaacatt
     1201 tttccttctt gggtacgttc cacaatcata tcacgttcca tttctgctac actcaacaac
     1261 gtccgtgtca ccattcgccc catggacgta ttttcaatcg ttcctagatt caaaaccttg
     1321 atcgttacgt tatcatcgag taaaggttca atgatctcta atgcttctct cgtgttacga
     1381 gcaaatcgat caagcttcgt aacaactatt tcatctccag gcttcagacc agccaacagc
     1441 tgattgaatt ttggccgatc ccgttttgta cccgtaaatt tttctgaaaa aatattcttc
     1501 ttctctacgc agttctcaag caactgttga atttgttctt ttgtactttg agaagcggtt
     1561 gagacacgtg catatccata tttcgtcatt tggtccacct acttttacaa atcatctatc
     1621 gtatactcag aatacccttc atagaaatag ctgacatcga ttcctttcat atataactcg
     1681 cgatcgtcaa ttttatcagt cagagcattg gaaattagat agcgtatttc caaatcgttt
     1741 atgggacttc gctccattgc agaaagatag tctgctttat cgattaagtt ccaatctaca
     1801 actttttgca attcttcctt taaaatgaga tccagccaaa ttctagtact tcggccattt
     1861 ccttctcgaa atgggtgagc gatattcatc tccacatatt tcttgataat ctcttcaaaa
     1921 gaagtttgtg gcatctgatc aatatgctgt aacgaatgtt ctaaatacat aactggtgca
     1981 aatcgaaaat ttccttttgc aatattgacc gatcggattt ttccggcaaa atcgtaaata
     2041 tccgaaaata gaaattcatg aatttcagat aaacctttaa atgtaccaac ttcgatttca
     2101 tttatttttc cacaatcgta caattctttc gctttttgtt tactgagttt ttcttcaacc
     2161 ttagccaact caacttgatt ggttaatccc aatttattct ctaaaatcat gcgaaatctc
     2221 cttaacttta tgacataagt ttatgaaaca ctctcaaccc cattatatcg cgattttgtt
     2281 tatgtgtcaa aaactattaa gtttctgacc cccgatagat ttttcaagtg tctctatttt
     2341 gcacagtcta gt
//
LOCUS       DACDIE010000147         2322 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.151, whole genome shotgun sequence.
ACCESSION   DACDIE010000147 DACDIE010000000
VERSION     DACDIE010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.151"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            131..1993
                     /locus_tag="IV250_002817"
     CDS             131..1993
                     /locus_tag="IV250_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8770041.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            1993..>2322
                     /locus_tag="IV250_002818"
     CDS             1993..>2322
                     /locus_tag="IV250_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8770042.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          834 a          345 c          455 g          688 t
ORIGIN      
        1 tcgtctttat aatagataaa ctctgtaata ttagttaaaa agctataagt tgcacttagt
       61 taagtgcaaa catttcatat tgtcgttagt ttagaaaacg cttatattag caatgtaagg
      121 agagatgata atgcagcagc taacaaaaag agaaaggcag cttttgattt acttggtgga
      181 aatgtctgaa ccggtaacaa tcaaaaagtt ggctgaaaaa gaaaatgtta gtgttcggac
      241 gataaaatac gatctagatg atataagaga gtggctgaat gagcggaagc aagtcctata
      301 tagcaaacgt agtcagggaa tttggatgaa tattagtagc tctgatcgaa tcaaattaaa
      361 aagcgaattg atggatgtta atcgttttga aatatttgca gatcaaagtc tccgaatcaa
      421 acgtttacta attatgcttt tagtaacaaa agaagctatt actggaatga aaatggccaa
      481 cgcattagat gtcagcaaag atacgatcat gaatgattta gatgtgctgg aaaataattt
      541 tcttaaggaa ggattgacct tgaatcgtca ggcgcgaaag ggtttttgga tcataggaga
      601 agaacgtcta gttcgattaa cgattgaaga gattcttcaa aaagagttca cggattatga
      661 catttataaa ttaatgtcat tgttattgaa tggcggtgag actgaatact ttgaaatgta
      721 ttctgctact gctacaccta tccaagaggt atttaatcaa gtaatcattc gaatgcgaca
      781 cttactggaa tttgaaaatc tagagaaact gaattacgcg gaattactaa atattctgat
      841 tcgagtgaca attgctaccg ttcgcttacg caaagaggct acgattggtc gttatcaatt
      901 ggttgctgaa caagaattag acaaaaacga tctttcttat cgcgtcatgc agcaggtctt
      961 tgattattat cagttgccac tgttcgagga tgaatatcat tatatctata gtgatacatt
     1021 cgacacgatt caccagcaag acgtcatgaa actagttgag aatgtgattt ataaagtaag
     1081 taaagcaatc aaccattctt tttatcaaga tccacaattg ttgacgaatc tgtatgctca
     1141 tttatccatg cgattatcga gaaagaaaaa atttgtcaat gaatataatc cttttaaaga
     1201 agatatcaag cagaaatacc cggaattatt tgatgcgatc aagcaagcag ttcgacaaga
     1261 aataagtgaa caaaatattc tgatcaatga ttctttcgtg gcatatattg ccttacactt
     1321 tttggtttcc tacgaaagag aagaagatct acgaagcgtt agagccgtct acatttgttc
     1381 aacagggttg ggtgtgacta gtctgatcaa gcaaaagatc aatgaagaat taaataatat
     1441 cactatcgtg gcatttgctt cagtattgaa tgcgcaagaa atgattgcta ccaaagatcc
     1501 tgatttggtg attagtattt ttccaattaa tggcatcgat cgtcctttca tcaaagtcca
     1561 tccattaccc actgaacagg acttgaaaaa aattcaacaa ataataaaaa aaatacttgc
     1621 ttcttccaat cagcaggaaa ggcataactt gaatcttaga gaaacgtcca tcaataaaga
     1681 tactttagaa gactttagca aggaattgat tttgaaagct tatacgattt atgaacgttt
     1741 acttgtcatt ttggaagcag aattgatcca agaatatcga gaagcttttt tgcttcatgt
     1801 attcttaatg gttcatcgta tcacatttga tcaacaatat atggcagaag gtcgtcaagt
     1861 agaccagatt attttagaca aaggaacact ggtaggaaaa atcgaagcag tttttgcaga
     1921 aaataattta ggaatcaatc agtcagaaat cagtgcatta ttctcatata taaaagaagg
     1981 agcgtcaagt tagtgaaaat gtcagacgag gctttaaaaa ttatcgagga aagtcaaaat
     2041 cgggaaaagt taaaacaagt gattgatcta attgaagagc gattagtaga acatgctatt
     2101 gtaccaaccg agttgcagtg gaccattcta attaatcact tgaatgagat gctgaaacgt
     2161 tctcaaagaa aggaaacgat tcccgtagtc gatcgggatt tgttcaaaga agtatccaaa
     2221 gaagccataa ctatttctga tgaaatagtt agaagtatcg gtaatttaac cgatgatgaa
     2281 atatatgttt tgtcgattca ttttgaaaca gcaaaaaata at
//
LOCUS       DACDIE010000148         2318 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.152, whole genome shotgun sequence.
ACCESSION   DACDIE010000148 DACDIE010000000
VERSION     DACDIE010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.152"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            398..583
                     /locus_tag="IV250_002819"
                     /pseudo
     CDS             398..583
                     /locus_tag="IV250_002819"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            777..947
                     /locus_tag="IV250_002820"
     CDS             777..947
                     /locus_tag="IV250_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8770043.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(1371..>2318)
                     /locus_tag="IV250_002821"
     CDS             complement(1371..>2318)
                     /locus_tag="IV250_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen binding domain-containing protein"
                     /protein_id="HAP8770044.1"
                     /translation="PAGAVFELQNREGETLQTGLTTGEDGKLAIDGLAPGAYQLVETQ
                     APTGYELDATPIEFEIERSQTAVVELTKANRLTPGGVVLTKIDDQSGEVLQGAVFELQ
                     DANGTVLQSGLTTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFEIERSQTAV
                     VELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDG
                     LAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTS
                     SGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT          641 a          493 c          400 g          784 t
ORIGIN      
        1 ttatttttta cagtcatcta tgacaggttg taaatactat ggaacatcat atggcgttgt
       61 cattttcttg tcaactattt ttcctattca tgtttttata aaaaaacata tcgttttctt
      121 gtatgattaa atcaccacaa aataaacaac tgagagaacg atatgcttta tgaatggttc
      181 tatcaaaaaa attctgagat caatagacaa aaatttgatg attacagagg tctcttacga
      241 aaccttacag aagaaaaaaa cattgatcgt tgctgcagtt ctcttccctg ctccatgtgt
      301 ctgtagaaat tgtggttctg ctgtggtaga tggaaatagg aaattaatgg tggtgaaaaa
      361 caagaagaag gaaaccattg tccactttga ataatataat catatgccaa tggttatacg
      421 cctaaaaaag cagcgctata cctgtaaaaa ctgtcgaagc cattggaacg ctcaaagtta
      481 ttttatccgt ccaaggcact cgatttcaaa tcatgtcaga cataaaatca cttctttact
      541 gactgaaaaa gtgtcgttat tttttatttc gaaaagttgt taggtatctt tgacaaccgt
      601 tattcgcaca ttgaaagagt tgagaagcta tttaccaaag tcttccaaaa gaattctgtc
      661 ttgagtgttg agggttgtga atttcgttct cacgtttccg ttgaggataa actgagtttt
      721 atttccgcgg atggcgagac agaaaaatta atcgagaaaa tttacctaca caaaaaattc
      781 ctcgattaac aaactatttc cttagctgca ctaatcctaa agaaatagaa ttcttagtta
      841 cagacatgaa cgccacctac tttcagctca ccaaacatgt tctaccaaat gcgaaattgg
      901 tgattgatcg attacttggt ttttccacgc ctttcaagga ggcgtaacct ttattcacga
      961 gttggttgaa gctttccgag acaaaagacc cgacttgttt ttctccttat tgacaaagag
     1021 ccattaaaac tagagaaaaa attacaactg atcgctgatt atttgtagaa agaaaatcaa
     1081 agtattaccc aataaacttg agtcattagg caaaaaacac tgcgaaactc aagaatgatt
     1141 ttcgcagtgt ttttgtatat tttttattaa ctagtattac tacaaaggtt tatttttcac
     1201 ttctcaacgc agcacaaaat tgtctttact tatttactgg ttgggttcaa ccttctctac
     1261 tccgatacta atatttgttt gtatacccct tctaaaatcg gtagtttcca ttcaattgat
     1321 gggtactagt tgattttaaa aactatgctt attcaatttt ttattttagc tcatatattt
     1381 cgtttctttc taaattttat actgatgaaa acaagtagca cacccaatat tgacaatata
     1441 aactggctaa tgatggtttc tccagttttt ggaagatgct tatttccact gcttgttgga
     1501 ttgtttcctc ttatatctga gctatcagca acttttctat tctcttttgt taaaaagatc
     1561 gccacttctt gttctttttc gatcttgaaa gttactggtg tcgcatccaa ttcatacccc
     1621 gttggcgctt gagtttctac cagctgatat gctcctggtg ctaagccatc tatcgccagt
     1681 tttccatctg ctcctgtggt tagacctgac tgcagaacgg ttccattggc atcttgtaac
     1741 tcaaaaacag ctccttgcag gacttctccg ctttggtcat cgatctttgt caacacgaca
     1801 ccgcctggtg taagtcgatt ttctttcgtc aattctacca ccgctgtctg acttctttcg
     1861 atctcaaact cgattggtgt cgcatccaat tcataccccg ttggcgcttg ggtttctacc
     1921 agctgataca ctcctggtgc taagccgtca atcgccagtt ttccatctgc tcctgtggtt
     1981 agacctgact gcagaacggt tccattggca tcttgtaact caaaaacagc tccttgcagg
     2041 acttccccgc tttggtcatc gatctttgtc aacacgacac cgcctggtgt aagtcggttt
     2101 gctttcgtca attctaccac cgctgtctga cttctttcga tctcaaactc gatcggtgtc
     2161 gcatctaatt cataccccgt tggcgcttga gtttctacca gctgatatgc tcctggtgct
     2221 aaaccgtcaa ttgccagttt tccatcttct cctgtagtta gaccggtttg tagcgtttct
     2281 ccttctcggt tctgtaactc aaaaacagct cctgcagg
//
LOCUS       DACDIE010000149         2311 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.153, whole genome shotgun sequence.
ACCESSION   DACDIE010000149 DACDIE010000000
VERSION     DACDIE010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.153"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            25..702
                     /locus_tag="IV250_002822"
     CDS             25..702
                     /locus_tag="IV250_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP8770045.1"
                     /translation="MNLVPLKNFNAKQMDLFFALCARMKDRGLKKVRFSFEELKELSD
                     YKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEINKTKKFVEVSVNPD
                     LEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYYVVRIEEFRSLMDIP
                     EYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLEFTFSSLTSDLPKIS
                     LRNWLEE"
     gene            complement(839..1255)
                     /locus_tag="IV250_002823"
     CDS             complement(839..1255)
                     /locus_tag="IV250_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770046.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1258..1542)
                     /locus_tag="IV250_002824"
     CDS             complement(1258..1542)
                     /locus_tag="IV250_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770047.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            complement(2242..>2311)
                     /locus_tag="IV250_002825"
     CDS             complement(2242..>2311)
                     /locus_tag="IV250_002825"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAP8770048.1"
                     /translation="CSLLVGLLSALFSYWLNNRNKK"
BASE COUNT          748 a          355 c          416 g          792 t
ORIGIN      
        1 ttaacagtca aatatcaaaa tgaattgaat ttagtaccgc tgaaaaattt caatgctaag
       61 cagatggatt tattttttgc attatgcgct cgtatgaaag atcgtggttt gaaaaaagtt
      121 agattttctt tcgaggaatt aaaagagtta agtgattata agatgactgc tacaaaagcc
      181 tttgtcggtg atttagaaaa gctctataaa gatatgctta atcttactta ccgtactgaa
      241 gatgaggatt ctattcgcta ctttgttttg tttactggtt ttgaaatcaa taaaacaaaa
      301 aagtttgttg aagtcagtgt taatccggac ctagaatata ttattaatgg tttaacagct
      361 gaatttagta gatttgaatt atctgctttc acgtcgttgc gttctacgta tgcaaaaact
      421 ctattccgac tgcttatgca gtaccgttct actggttatt atgtagttcg tattgaagag
      481 tttaggagct taatggatat cccagaatac tatcaaatgg gtaacattga tcaaaaagta
      541 ttaaatcctg cattacgaga actaaaaaaa tacttcaatg atcttaaagt cacaaaaata
      601 aaagcaaaaa agggcaataa aatcgccaaa ttagaattta ctttttcaag cctaacgtca
      661 gatttgccaa agatttctct gcgtaactgg ttagaagaat aaataaaaaa gaactcgccc
      721 cccttacatt aagcaagtag acaagtccct tacaaccgac taagattgct agtacattct
      781 agcatattgt atggggcttc taaataaaaa acagatagga ataactccta tctgtttttt
      841 atcgttttag aaaatttgtt gggtcgaaat ttctagaagt gactgtttga ctggtttctt
      901 tttgatgatt ctttggtcga tttcgatttg atggatttag tttagcttcg attttttctt
      961 tcacttcttg atatacttta gaatttgtgc caataagaac gcctgtatct tttagatata
     1021 aatcagcaaa ttctgcgatg atttcagtca tgatggaaat ttcttttccc atagcattta
     1081 gttttgcctg attgctggta gtggtcgtgt tggtatcggg ttcataactc atacataaaa
     1141 agcgaatggc ttccgagtag ttattgatat ctggattttt ttcgatgatg gaatctaaaa
     1201 cgtcaacatg actagggtac aaagtaacat gtttttggac agaagttttt ctcatggtta
     1261 tatcattcct ccgataagca tttcaatgat ttgattattt gtgtagacat actcgcttaa
     1321 tatttgagta ttttctgttt ggatagcaat aaatttatcg agcaataaat cgaattggtc
     1381 ttttttgtaa gtcgtaagtt gacggtcaaa atcactttcg attaaaactt ttagatattt
     1441 atttcgactc catttttctc ctgttttatt cgtgagttct tggcaacgtt tgtctattgt
     1501 tgcaacaaac tttggatcaa tattccgaac taaaatgtcc ataaaaaatc tccttttgca
     1561 attatgtata atttatgtat accatacaaa tactatattt gtatagctgt tttttgccaa
     1621 aatgctatac ataagctacc cacgatggcg aaaaagtgat atcaaatttt tttctgagaa
     1681 acgtgatagg cacagcctat ctacccactt tttttagaaa gtgggtacac gtttccttat
     1741 gctctttaaa attcgggggg gagtgctgca cgtgtaatcg ataaccaaat ttaacagacg
     1801 aaaaattgtt ggaatataaa aatatgacac ccgaaagaaa acgatcgttg gaaaaattaa
     1861 tgcaactagt gaacgaagac ggcggaattt cagacgcagc ttttgacgca tggagcgaat
     1921 ttcatggaga atataattaa gtgggtttaa atggctctaa aatcgcttct aagagttttt
     1981 tagcttggac caatgaaata caccaaaaac taaataaaac gtcttaaatc gaaaaatacg
     2041 agctctcaga gcgactttat ttgtaaatgt tgcatataag aaataattac ggattttaga
     2101 cttttgtagg atatttttga aataaatatt tatttctgtt ataatttgat tgcaaataag
     2161 tgaacacgaa acacacgaaa tccccagaga ggtcggattc tctggggctt tttttgtggg
     2221 ctgagtcgcc ttaattaagt tttacttctt gtttcggtta ttcaaccagt aactgaacaa
     2281 agcgcttaaa agcccaacaa gaagggagca a
//
LOCUS       DACDIE010000150         2310 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.154, whole genome shotgun sequence.
ACCESSION   DACDIE010000150 DACDIE010000000
VERSION     DACDIE010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.154"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..911
                     /locus_tag="IV250_002826"
     CDS             <1..911
                     /locus_tag="IV250_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002393091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8770049.1"
                     /translation="ISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQVANNRLNEIF
                     AIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPSHSKTAIVGV
                     SGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQESFFFSGTIIE
                     NLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGGQRQRLALAR
                     ALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAKACDQILVLH
                     EGHLVEKGTHDELRFADGIYQKLWEI"
     gene            880..2295
                     /locus_tag="IV250_002827"
     CDS             880..2295
                     /locus_tag="IV250_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAP8770050.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
BASE COUNT          898 a          343 c          417 g          652 t
ORIGIN      
        1 gaattagtct aggtcaactt atcacataca atgctttact agtatttttg acagatcctt
       61 tacaaaacat tattaattta caagtgaaaa tgcaaacggc tcaagttgca aataatcgtt
      121 taaatgaaat tttcgcgatt gattcagaaa aatcgaacca gtcttttgaa tatatgcttt
      181 ctgaacaatt atttcaacaa gatatcactg taaaaaacgt ttcattctcg tatagcatga
      241 aggccccaac gttgaacgaa atttcatgta caattccttc gcacagtaaa acggctatag
      301 ttggagttag tggttctgga aaatcaactc ttgcaaagtt atttgttcgt ttttatgaag
      361 catcagaagg atcgatccat tatggaaaca ttaatcactt agacattcct tttcagatat
      421 tacgcgatcg agtgacatat ttgcctcaag agtcattttt ctttagtgga acaatcattg
      481 agaatcttat attcggttta gatagcccac caagtttcga acaaataata gaagtttgtg
      541 agatagtcca actaaatgaa tttattaatc aacagccatt acgttacgag acattgttgg
      601 aagaaggtgg aaataatctt tcaggagggc aacgacaacg attagcctta gcacgggctt
      661 tattaaaaaa tccggacata ttaattttag atgaagcaac tagtggatta gataccttgt
      721 tagaacatgc aattatgaat tatctcctca atttaaaaga aaaaacgatt atttttattg
      781 cgcatcattt accaatagca aaagcatgtg atcaaatttt agttctccat gaagggcatt
      841 tggtagaaaa aggaacgcac gatgaacttc gttttgccga tggtatctat caaaaattat
      901 gggaaatcta ggagagggaa acaatttatg cagaataata attggttcga acattctagt
      961 atctatacta aacaacatca tagcttctac cgttgggtgt tataccctgt aattcttttt
     1021 ttatgtatta ttggattgtt tttaaccttt gctaaaaaag aggttgttat tcggacaacg
     1081 gctcaattga accccgaaaa aatagagaag ttacaagtgc cattagaagc taaaataatg
     1141 gaaaacttcc taaaagaaaa tcaatttgtt cataaggacg atgtgttggt tagattggat
     1201 tgttctttaa tagagaatga aaaggctcag atagaacaag aaaaccaacg tattactcaa
     1261 caaataaaga tggctcagct atttattgaa agtataagta aaggaaaaaa tttgttttca
     1321 acggatgaca gttttggcta cagtaatcaa ttaaagagca tgttgtcaga aaaagaatca
     1381 ctccgctacg ctttgaagca aagtgaatta aatgatcaaa agcaattaga agtatacgaa
     1441 aagacaaaaa gacaactaga aaaacaaatt gagagttcag atagtaaatt acaagaatgg
     1501 caacaagtac aggtagcttg gagtaataat caatcattaa aagatttttc aaaagaaatg
     1561 atggcaaact atgagaattg gcaagaacaa ctaaataatg tttctgatga tcaaaaaaat
     1621 caagtgaaac tgacaatttc agcaagcata aatgaacaaa ttgagcaact aaaaaaagaa
     1681 gtagaacagt atcagtcaga aaaagctaaa ttagttaaac caactacttc tgagaatgac
     1741 agaattagtc aaacggaaaa aggaaagcaa gagctagaac aagcaataac aactgtaaaa
     1801 caaacagtta cagagctaaa tgaaaaacaa gagaagaacc agtcagttat caaaagtttg
     1861 gacgatcaac tttcaaaagg aatactaaaa gctcctgtat ctggaacggt tcatttaaat
     1921 gaagaaacaa agggacaagc agaactagcc aaaggaacag tgcttgcaga aatttatccg
     1981 atccacgaaa aatcaaaaat gaaatttaca gcacttttac ctgcgaatga aagtacctat
     2041 attaaagaag ggatgaaggt tcattttaaa ttggatcaaa aaggtggggc tccgattacg
     2101 atcgacggaa ctttagatga aatttcagaa aattctactg ccactgagaa aggtgttttt
     2161 tatgcagtga aaggaacgtt aaaaacaact caatcgtttc cgtttcgtta tggattaact
     2221 ggagagttgt ctttaattgt aggagaaaaa acatattggc aacaaattgt cgatgtgttt
     2281 ctaaataaaa aataattatt tttactaaga
//
LOCUS       DACDIE010000151         2298 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.155, whole genome shotgun sequence.
ACCESSION   DACDIE010000151 DACDIE010000000
VERSION     DACDIE010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.155"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(89..1399)
                     /locus_tag="IV250_002828"
     CDS             complement(89..1399)
                     /locus_tag="IV250_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HAP8770051.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            complement(1431..2255)
                     /locus_tag="IV250_002829"
     CDS             complement(1431..2255)
                     /locus_tag="IV250_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002579255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8770052.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
BASE COUNT          744 a          435 c          352 g          767 t
ORIGIN      
        1 gtttactaag tgttttgtca atatttttac taaatttaac taagaaaaga gtaaggaatt
       61 gaaaattcct tactctttca gcttcttttt aattatattc aggtaaatag tccccatgtg
      121 cttcgattaa atcatccact aaatttttga tatctgttaa agacaattca gatgatagat
      181 gtggatctaa atacgctgct tgataaattt ttgatttatc tagtgttaaa gcggcttcta
      241 ctgtcagttc ttgaacattg atattcgtcc gatttaaagc agctaattgt gttggcaatt
      301 caccgacata ggttggttgc acgccatttt tatcgactaa acaaggtact tccacacaaa
      361 catctgtcgg taagtttgtg attaagcctt tatttaagac atttcctgca atcactgacg
      421 gagttcctgt cgtaatagaa tccatgatat atgaagcata ttcacggctt ctcgtatgtg
      481 ttaactcccc attatttaca atgtcctctc gcatgctttc ccaatcatta atttgttgtt
      541 cacaacgacg taagtattca tccaacggaa tccctaattc gtcaatcaat tctgggtatt
      601 ttgatttaat aaagtatgga tggtattctg cattgtgttc acttgattct gtaatatagt
      661 aaccaaaacg tttcaacaat tcaaaacgta cagaatctgg atgtggattt ggtttttctg
      721 cagctaactg acgaattgtt ggatagaaat cttccccatt ttttgtaatt tccaacaaga
      781 atgccatatg attaatgcct gcaattttcc attgaaagtc atttaagtca tattgatcgg
      841 ctattcctaa atgctcaaac aattctggta cgcatacttg aacgctatga cataaaccaa
      901 cagtcttaat actcgtcgct tttaaaacac ccatggttaa catcgccatc ggatttgtat
      961 agttcaataa ccaagcattt ggacaaactt cttccatttc tttagcaaag tctaacatta
     1021 ctggtagtgt ccgtagggtt ctaaaaatcc caccaatccc catggtgtca ccaatagttt
     1081 gtcgcaaccc atatttttta ggaatatcaa aatcaatcac tgttgaaggt ttataaccac
     1141 ccacttgaat agcattgata acaaaatcag cctctcttaa cgcttctttg cgatcataat
     1201 aagaaacaat atcagcacga ttttcattcg aatttttatt aatattttta agcattaaat
     1261 ttgattcttt tagtcgtttt tcatcaatat cgtacaatgc aatatgtgca tcttgtagac
     1321 ttggagttaa catcgcatcg cctaatacgt tttttgcaaa aatagtactt cctgcaccaa
     1381 taaatgtaat ttttgtcatg tacttactct ccaatcacta attaatagtt ttataattta
     1441 ctacctgttg tagcaattcc ttcaatgaat tttctttgga aaacgatgaa tatcacaact
     1501 attggccaaa ttgctaataa cgaacctgcc attaattgtg ggaagttcgt ggcatattgt
     1561 ccttgtaagt ttgctaatcc cggagctaaa ggcatcttat caattgaagt attgacaatc
     1621 aatggccaca ttaaatcttt aaatgcaaac aacgctgtga atattccaag tgcaaccaat
     1681 cctgatttag caagcggcaa cataattttg aaaaagatcg tccaaatatt ggcaccttct
     1741 aaataggccg cttcctccaa ttcatcaggt aaacctaaga aaaattgtct taataaaaac
     1801 gtaccaaacg cactaactac ccctggtaat attaatgctg gaattgtatt taaaataccc
     1861 aatttttgtg ctaataaata ctgtggagta ataaaaatgg aaccgggaat catcatttga
     1921 ataagtacta agctgaaaag gatatttttc cctggaaaac ttagacgcgc acaagcatat
     1981 gcagccattg ctgaaaataa aaccgagcca atcactcgtc caacaaccat taaaaatgta
     2041 ttgatataaa aagccatgaa tggtaataaa tcaagtactt gttgatagtt acttagctga
     2101 aatttctcag ggaaaattgt aggtggaatt tgggtagatt caaccaacgt tttccctgaa
     2161 gttaaaatca tccaaataaa aggtaatgcc attgcaatac cacctaaaat aagcatgata
     2221 tggattggcg catttgaaaa aaatttattt ttcataattt gatagcctcc ttaatagttt
     2281 acccactttt tctgcaac
//
LOCUS       DACDIE010000152         2142 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.156, whole genome shotgun sequence.
ACCESSION   DACDIE010000152 DACDIE010000000
VERSION     DACDIE010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.156"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            102..1397
                     /locus_tag="IV250_002830"
     CDS             102..1397
                     /locus_tag="IV250_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP8770053.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFKLRIMIRHGKTFLTK"
     gene            complement(1511..1909)
                     /gene="spxA"
                     /locus_tag="IV250_002831"
     CDS             complement(1511..1909)
                     /gene="spxA"
                     /locus_tag="IV250_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HAP8770054.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          673 a          418 c          370 g          681 t
ORIGIN      
        1 ggtgaacttc accaacatct tttttgtata aaaaaatata gaaaagacac cctgaaatcc
       61 tttagaatta atttggctac caattcatag gaggattcaa gatgtctcat actcagctta
      121 tcaaagatac cctaaatatt ctagacctaa atatctattt cgaggaaaat tgtttaacta
      181 aagaaaaata caaagggcaa atttgcttga tttacaaagg aactcttctg tacaagccag
      241 aagagtgttc ccattgtctt tgtgtcgtcc cctctaggat cattcgttgg gggacgacaa
      301 ccgttcggtt gttactgaat gatgtgtctg agtatcgcac ttatctggag ctgaagaaac
      361 aacgatttaa atgtaagtct tgccaaagaa cattcgtagc ggatacctct gttgctgaaa
      421 aacactgttt cattagtcaa aaagttcgtt ggtctgtcat tgctcgctta aaagagaata
      481 cttccatgac ggaaatcgct cgtcagaaaa atatttccac ttcttcggtc tatcgtgtca
      541 tgaagcgatt ctaccgccca ttgaatcctt tcaaacagac tttgccaaag gtcttatgct
      601 ttgatgaatt caaatcggtt cgaagtgttg ccagtgcaat gagttttatc atgatggacg
      661 gacaaagcca tgcattattg gatattgtcg aaaatcgacg cttgccttat ttagaacgct
      721 atttttctcg gttttcatta gcgactcgtg aagccgttca attgattgtt attgatatgt
      781 atgcgcctta tgtttcgttg gtgaaaaagc ttttcccgaa tgctcagttg atcatcgatc
      841 gtttccacat cgtccaacat attggcagaa ctttcttgaa tcatcgagtg aaagaaacca
      901 cggtacgact taaaggaaat tcccactctc aacgagggtt agggaaaaaa ttgaagcgct
      961 attggaaact cctacaaaaa gaagaagcaa agttaaacta tgaaaaacgg gtttggcgtg
     1021 ccagttttaa agattatttg actgaaagtg agattgccga tcgactgtta gctgcttgtc
     1081 ccaacttacg ccaaggctat cagttatacc aagacattct ttatgcggta aagaaaagag
     1141 atcagtcact atttgaggat tgtttaacta gagacattag cggtctgcca gaaacctata
     1201 caaccacttt acgaacgttt aaaaagtttc tgccacagat ccaaaatgcg cttcactaca
     1261 gctattcaaa cggaccattg gaatgcttaa acaaccacat caaagtatta aaacgcaatg
     1321 cgtacggctt tcgcaacttt tacaatttca aactacgaat tatgattcgt catggcaaaa
     1381 catttttaac caaataaaca gaacagaaag aggttttctt ctttctgttc tgtccaaatt
     1441 attctaagat ttctctttac caacatcatt tgacaaagag ccagtctttt ccaagtttcg
     1501 tattatcctt ctataatcca gccatcaatt gtgcttgacg taattctaat tgtcgtacat
     1561 cgcgcggtag gaaccgacga atttcatctt cattaaaacc aacttgcaga cgtttatcat
     1621 caatcatgat tggacggcga agcaatccag gattgttttg cacaagttcc aataattctt
     1681 gtaacggcag atcatctaaa tccatgttca gtttttggaa tactttggaa cgtgtcgaga
     1741 tgatttcttc tgttccgtct tctgtcattt gtaaaatcgc ttttaattca gaactgttca
     1801 atggttctga aaaaatatta cgttcaacaa atggaatctg atgttcttgc aaccacgcac
     1861 gagcctttct gcaggaagta caacttgggg aagtatataa tgttaacaac gtgcaccact
     1921 ccttttcgtg ataagaatca cttttttgta acagaagatc ataataatct cccatctgtt
     1981 tctattatac ataatcaact tttaaatagc tacctttttt tcataatttt tttaaaaaaa
     2041 tgaaagttat ctaaggttaa tatacagtaa aaccagcatt ctcgcttttt ttatcattta
     2101 aaaattagag ttttcaaatc taattcaaat tttctaaaca ta
//
LOCUS       DACDIE010000153         2102 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.157, whole genome shotgun sequence.
ACCESSION   DACDIE010000153 DACDIE010000000
VERSION     DACDIE010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.157"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            55..276
                     /locus_tag="IV250_002832"
     CDS             55..276
                     /locus_tag="IV250_002832"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770055.1"
                     /translation="MKQNKKTPSAKGALITLNFKDQKFSAVVDEIHPQEHQKNSFATD
                     EKKTQKRSEKVLRIIQFLLEKIRHFGSLF"
     gene            403..1179
                     /locus_tag="IV250_002833"
     CDS             403..1179
                     /locus_tag="IV250_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAP8770056.1"
                     /translation="MNTPQIFNFEQHEVRTVTIHDEPFFVGKDVAKVLGYQNGSRDIN
                     RHVDVEDRQNYQNGTFESPRGLTIINESGLYSLILGSKQPNAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATKLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMRQGYTKSHTTEIPK
                     SDGGTKVVMNTKWTQKGRLFIYESLKKEGYIPEIDLLEEE"
     gene            1176..1370
                     /locus_tag="IV250_002834"
     CDS             1176..1370
                     /locus_tag="IV250_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8770057.1"
                     /translation="MTMSQEFILKVRIQLAKYGKSQNWLADTIGISRPYMSDIMNGRR
                     KPDKQIKPIEAALAELEKEK"
     gene            1372..1656
                     /locus_tag="IV250_002835"
     CDS             1372..1656
                     /locus_tag="IV250_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770058.1"
                     /translation="MQITIPDNLVVSELTTQITNAVLNSLDERLHLMNKSVELPPYPN
                     KSEVKKVLGIGDDKLTHWISLGLKTQQWSKLDIRIERSELQRFLKENFEF"
     gene            1677..1865
                     /locus_tag="IV250_002836"
     CDS             1677..1865
                     /locus_tag="IV250_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770059.1"
                     /translation="MSYTLQQEHQILGLIKQRRKQLQDDRAALRKSDELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
BASE COUNT          808 a          330 c          401 g          563 t
ORIGIN      
        1 taaaaattag ctaattttat tagcttacat atttaaagaa aaggaggaaa catcatgaaa
       61 caaaataaaa aaactccttc cgcaaaagga gctttaataa ctttaaattt taaagaccaa
      121 aagtttagtg ctgttgttga tgaaattcat ccacaagagc atcaaaaaaa ttcttttgca
      181 actgacgaga aaaagactca gaaacgctct gagaaagttc ttcgaataat tcaatttctg
      241 ttggaaaaga ttcgtcattt tggaagtcta ttttgaattc tttattaaca gtcttgaata
      301 cggtttgttt gaataaggcg tcgacatcca tattaaattc ttccatacta ttttcacctc
      361 gctttctatt caaaattata tcaaagaaag gaatgagcaa aaatgaacac accgcaaatt
      421 tttaactttg aacaacatga agtaagaaca gtaacaatac atgatgaacc attttttgtt
      481 ggaaaagatg tggctaaagt tctagggtac caaaatggta gtagagatat taacagacac
      541 gtcgatgtag aagatcggca aaactaccaa aacggtactt ttgaatcgcc tagagggtta
      601 actattatta acgaatcagg tttgtactcc ttaattttag gaagcaaaca accaaatgct
      661 aagaaattta aacgttgggt gactagcgaa gtgttgccag caattagaaa acatggaggt
      721 tatctaactc cagaaaaagt agaagaagct ttgcttaatc cagatacaat catccaatta
      781 gcaactaagt tgaaagaaga acgtactgga agactaatcg cagaacaaaa aattgcagaa
      841 tacgaaccca aaatttcgta tttggatagt atcttatctt ctacagattc agtaactatt
      901 agtcaaatcg cggctgatta tgggatgtct ccacaacaga tgaacaaact acttcataag
      961 ctaggtattc agaaaaaagt aggcaaccaa tggttattat gcaaaaaaca catgagacag
     1021 ggttacacaa aatctcatac aactgagatt ccgaaatctg atggcggaac aaaagttgtg
     1081 atgaatacaa aatggactca aaaagggcgt ctgttcattt atgagtcgct gaaaaaagaa
     1141 ggatatattc cagaaatcga tttattagag gaggaatgac gatgtcacaa gaatttattt
     1201 tgaaagtcag aattcaatta gcaaaatacg gaaagtctca gaactggtta gctgatacta
     1261 tcggaatttc tagaccatat atgtcggaca ttatgaatgg gcgtagaaaa ccagataaac
     1321 aaatcaagcc aattgaggca gcactagcag agttagagaa ggagaaataa catgcaaata
     1381 acaattccag ataatttagt agtttccgaa ttaactacac agattacgaa tgctgttctt
     1441 aattcattgg acgaacgatt acaccttatg aacaaatcag tagagcttcc tccatatcca
     1501 aacaaatcag aggtaaaaaa agttttaggc attggtgatg acaaattaac acattggata
     1561 agcctaggct taaaaacaca gcagtggagc aagttagaca tcagaattga acgatcggaa
     1621 ctccaaagat ttttgaaaga aaactttgag ttctaaaggc aaaggagaat gattttatgt
     1681 cctacacatt gcaacaagaa catcaaattc taggtctgat taaacagcgt agaaaacaat
     1741 tacaagatga tcgtgcagcg cttagaaaat ccgatgagct atcagataga caagctgaac
     1801 taattgcttc tgaacttgag gatttgagaa tgctagaaat aaaaaatagg gagattagat
     1861 tatgaagaag acagacacac tttttatagg attcattttg gggttattag tgattgtagc
     1921 gcaccaaagt attatcggag gaagcttgtt cgcagcattg atggttttaa tcaatctgct
     1981 tgactcaaaa gaaaggagca actatggcac gagaagaagc gctaaaaatc ggtaaagtga
     2041 ttgctgataa ttggtgggca aatagccgtt ctattatttt aagcaagcaa catatcgaaa
     2101 ag
//
LOCUS       DACDIE010000154         1966 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.158, whole genome shotgun sequence.
ACCESSION   DACDIE010000154 DACDIE010000000
VERSION     DACDIE010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1966
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.158"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            344..934
                     /locus_tag="IV250_002837"
     CDS             344..934
                     /locus_tag="IV250_002837"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770060.1"
                     /translation="MNITISINKEFGELTEKKINRIAQSFNDIVNVQNIDSQIAFFQF
                     SDHALVLNRPNNEIAVAFSSKGLVNVSFKKLTILLDLLDLDNYGRTNISIEDIIDQEF
                     SVAEKSLDRLGINDSEIDGVGLRILVDTFGDNPKEFKIEPRIDDFSKLYIFCNQSQTK
                     VAELTEEFIDDSVKYLLDKSDEYKTFYLEKGGISGQ"
     gene            924..1091
                     /locus_tag="IV250_002838"
     CDS             924..1091
                     /locus_tag="IV250_002838"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770061.1"
                     /translation="MANKELVLIKEKKKRERAKRLEVIKSTGRIISVTTLVPFFQDDI
                     NVKEGEKKYDI"
     gene            1081..1755
                     /locus_tag="IV250_002839"
     CDS             1081..1755
                     /locus_tag="IV250_002839"
                     /inference="COORDINATES: protein motif:HMM:NF013970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HAP8770062.1"
                     /translation="MTFNPSLPFDISNKNWSDSNLFPNEMYLDTNLALMLVLRKPNFY
                     KVENFLKEYAITRGNDVFWSVLTESELFECIHVDTLKSNAKKFGYQPAKWKDMENELD
                     QKSFSMINNEADDRFTSAINVLKQYGEVLDDVNPSNQPDSRIIIKENARKIYTTYGGG
                     IKDAEHIAIANEYGINNILTNDSNSGNGFFRYPQQNIYGISKFISNGYNPNVKPNEYK
                     DLIKEI"
BASE COUNT          743 a          229 c          323 g          671 t
ORIGIN      
        1 tgtaagcaaa aatgtttacg aatttagata gtaaaaaata ggattgttct tagcatacag
       61 gaatttctaa tattgcgtta ctatctaaat cttctgtcca attagctaat aaaattagct
      121 aataatttag ctcatatata ttgactttcc tttgtgactg tcagataatt ttagtgtaag
      181 gagaatataa acgtttttaa ggtaactcct ttttgttagg ttttatctac catcacaata
      241 aaatagatgg atatatgcat aataaactca ttatgttttt tagtataagt atataaatcc
      301 actaattttt atatgctcaa ttatagcctg gaggaatagt agcatgaata ttacgattag
      361 cataaataaa gaatttggag agctgacaga aaaaaaaatc aatagaattg ctcaaagttt
      421 taatgatatt gtaaatgtgc aaaatattga ttctcaaatt gctttttttc aatttagcga
      481 tcatgcttta gtattgaatc gtcctaataa tgaaattgct gtagcctttt cttcgaaagg
      541 tctagtaaat gtttccttta agaaattaac tattttgcta gatttgttag accttgacaa
      601 ttatggaaga accaatattt ctatagaaga cattatagat caagagtttt ctgtagctga
      661 aaaaagctta gatagattag gaataaacga cagcgaaata gatggagttg gtttgagaat
      721 cttagtggat acttttgggg ataatccgaa agaatttaaa attgaacccc gcattgatga
      781 ttttagtaaa ttatatatat tttgtaacca gtcacaaact aaagttgcag aattaacaga
      841 ggaatttata gatgattcag tgaaatattt gttagataaa tctgatgaat acaaaacatt
      901 ttatttagaa aaaggtggta ttagtggcca ataaagaact tgttctaatt aaagaaaaga
      961 aaaaaagaga aagagccaaa agattagaag taattaaatc gactggtagg attataagcg
     1021 taaccactct agttcctttt tttcaagatg atattaatgt aaaagaggga gagaaaaaat
     1081 atgacattta atccttcttt gccctttgac atatcaaata aaaattggtc cgatagcaat
     1141 ttatttccga atgaaatgta tttagatact aatctcgctt taatgttagt gttgagaaag
     1201 cctaattttt ataaagttga aaactttctt aaagagtacg caattacaag aggaaatgat
     1261 gtattttggt ctgtcctgac tgaaagtgaa ttatttgaat gtatacatgt tgatacttta
     1321 aaaagtaatg cgaagaagtt tggatatcaa ccggcaaaat ggaaagatat ggaaaatgaa
     1381 cttgatcaaa agagtttttc tatgataaat aatgaagctg atgatagatt tacttctgct
     1441 attaatgtat taaaacaata tggtgaagta ctggacgatg taaatccatc taatcaacct
     1501 gatagccgca ttattatcaa ggaaaatgct agaaaaatat atacaacgta tggcggtgga
     1561 ataaaggatg ctgaacacat agctattgcg aatgaatatg gaattaataa tatattaact
     1621 aatgactcta atagtgggaa cggttttttt agatatcctc aacagaatat ttacggaata
     1681 tctaaattca tatctaatgg atataatcct aacgttaagc ctaatgaata caaggactta
     1741 attaaagaaa tttaaaatat taatttcatc tagttttttc tgtaaaaatt atttccgatt
     1801 aatataagta aattgaaaat aaattatcaa cttttagtgt ctttctagat aacaaactta
     1861 tacatcataa ccttagtgtg ttatcttagt atcgggaata tgagttatta tgataataga
     1921 atctattcaa ggctacctga aaaggtagcc ttttttaatc tttata
//
LOCUS       DACDIE010000155         1928 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.159, whole genome shotgun sequence.
ACCESSION   DACDIE010000155 DACDIE010000000
VERSION     DACDIE010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.159"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..367
                     /locus_tag="IV250_002840"
     CDS             <1..367
                     /locus_tag="IV250_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP8770063.1"
                     /translation="KKQIVPKEIETWWRIEMQLRRGKATDWHAMVRESLDSFASPHYL
                     PGDVKPVDRIMIKGLNQDHSEWAYISRNLKYRLRKLLKEESQNDELTNHLRETFKESA
                     NDLKIELDTWLLGLDVTEK"
     gene            567..770
                     /locus_tag="IV250_002841"
     CDS             567..770
                     /locus_tag="IV250_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770064.1"
                     /translation="MSQLGKCYSLSKSNRKVYTLFISDSAKEGFTLGFSISFKKGTKN
                     TSISHNNRELTDSQKINGLSGRK"
     gene            1330..>1928
                     /locus_tag="IV250_002842"
     CDS             1330..>1928
                     /locus_tag="IV250_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAP8770065.1"
                     /translation="MSKKGIIVNEWEKLNRISNRRLSVDDKVHPLKLSWSIDRITIVG
                     KLNDTIAYWTQNGVVFVDFEQLMRLNENQGYLKSVGTNGWQLFDQYGEDIAYIEMLKF
                     HPDKGRIDFNPNKIDEFLKGSMKQFIHDLFLDAHFSRADIACDILGVPDDFITQYRIV
                     DPVSFKPIYGRSGKLETAYWGSRASERQVRMYNKKLEQER"
BASE COUNT          626 a          293 c          453 g          556 t
ORIGIN      
        1 aaaaaaacag attgtaccaa aagaaatcga aacatggtgg cgtatagaaa tgcagctaag
       61 acgtggcaaa gcaacagatt ggcatgcgat ggttcgggaa agtttagata gctttgcaag
      121 tcctcattac ttgcctggag atgtaaaacc tgttgataga attatgataa aagggctgaa
      181 tcaagatcat tcagagtggg cgtatattag cagaaatttg aaatatagat tgcgcaaatt
      241 gttgaaagaa gaaagccaaa acgatgagtt gacgaatcat ttacgagaga cgtttaagga
      301 atctgcaaac gacttgaaaa tagaattaga cacgtggctt ttgggtttgg acgtgacaga
      361 gaagtgatac cgaataagaa aagtcagatg agtgagagta aatttacgtc aatgtttaat
      421 gacgtgagtt tacgataagc gattattttg cgtgaagcaa aataaagcgt aaaaggagtt
      481 gagagcgtga cacgctttcg gctccttttt tcgttgtcac ttgtgacagc gaataccctc
      541 ggagagcatt tggaattgtg acagccatgt cacaattagg aaaatgttac tctttgtcaa
      601 agagtaacag aaaggtttac actcttttta ttagcgacag cgcgaaggag gggttcacct
      661 tagggtttag tatttcgttc aaaaagggaa caaagaatac aagcatttct cataataatc
      721 gtgagttaac tgatagccaa aaaatcaatg gtttgtctgg tagaaaataa ttagcagatt
      781 tacaagtggg aaataaaaag ctgaaagaag aaaattgcat ttcaaactac attttctaca
      841 atagctctaa aatggcatat tttgcgttct gagcggtttt agcgtttatt tcgtttagtt
      901 atcggcacaa tcggtgaaac acgttagagg agcgaaaatg gagcctgtaa ggcgactggt
      961 agcggaacgg tcgtgttaga ttgtgtagcc gatagctgaa cgaaataaat gctggcagcg
     1021 ccattctatt tcggcaggag gaggctcaag gggttcgggg aatgaaattc cctgaggtct
     1081 ttttattttt tctagaggag tgctataatc aaagaaaatg agaaaaaaga gtaaaaaaat
     1141 agtcgagcag tgttgacgca ctctcgacct ttacggaaac tacttcacct agttccgtaa
     1201 ctcaagttca ttatacgaat atttttaggt ttcttcaagc tagagatttt tggatgtgga
     1261 tttttagagt tacgtgaact ctggaaattc tttccaagaa tctctttttt cgttgaggag
     1321 gagctatttt tgagtaaaaa aggcataatc gtaaatgagt gggaaaaact taaccgtatt
     1381 tctaacaggc ggttaagtgt tgacgataag gtacaccccc taaaattaag ttggtcaatc
     1441 gatcgaatta caatcgtcgg aaaactgaat gataccatag cttattggac gcaaaatgga
     1501 gttgtgtttg tcgattttga gcaattaatg cgtttgaatg aaaatcaagg gtatttgaag
     1561 tcggttggta caaatggttg gcaattattt gaccaatatg gagaagatat cgcctatatc
     1621 gaaatgttaa aatttcaccc tgacaaaggt cgtattgatt ttaatccaaa taaaattgac
     1681 gagtttttaa aggggtcgat gaaacaattc attcatgatt tgtttctgga tgcgcatttc
     1741 tcacgtgcag atattgcttg cgatattctc ggtgttcctg atgattttat tacccagtat
     1801 cgaatcgttg accctgtgtc ttttaagcca atttatggcc gttctggcaa attagaaaca
     1861 gcgtactggg gtagtcgtgc cagcgagcga caagtgcgga tgtacaacaa aaagcttgaa
     1921 caggaacg
//
LOCUS       DACDIE010000156         1857 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.160, whole genome shotgun sequence.
ACCESSION   DACDIE010000156 DACDIE010000000
VERSION     DACDIE010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.160"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..187
                     /locus_tag="IV250_002843"
     CDS             <1..187
                     /locus_tag="IV250_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770066.1"
                     /translation="EKTKRRTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRAK
                     RENVDSKGRQFKHWEKK"
     gene            187..501
                     /locus_tag="IV250_002844"
     CDS             187..501
                     /locus_tag="IV250_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770067.1"
                     /translation="MEELITKVEQWAKDKGLDQADSSKQMLKTIEEIGEVAASLARKD
                     EYGLRDGIGDVVVTLIILAMQNDMDLYECLNQAYNEIKGRTGKMVDGVFVKSSDLEDS
                     DE"
     gene            494..1024
                     /locus_tag="IV250_002845"
     CDS             494..1024
                     /locus_tag="IV250_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAP8770068.1"
                     /translation="MNKKVLIDKQALIDELMKIPGVGSNSDALETIKRFPSYEPQKPV
                     VPKFVAELLDYYRQSTDVDLLALLITFHDWYYRKTKDGEHEEAIDWLVDHPEIYMRAW
                     LFGYELEKEPLYCVIINGRYLVKVFSNTNVVILVPADEFSQYVTQKFQLTEKQIKAID
                     ERYWPFAVPVDEVVEG"
     gene            1021..1290
                     /locus_tag="IV250_002846"
     CDS             1021..1290
                     /locus_tag="IV250_002846"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770069.1"
                     /translation="MSDPYITKLEKLSEDFEKNKNKLTKDKLIELVRLVIRGAITREK
                     AQQNIEIFARSVRDGLEKKVKQNEQQTPQNNKTKKTGTECTKDKV"
     gene            1420..1707
                     /locus_tag="IV250_002847"
     CDS             1420..1707
                     /locus_tag="IV250_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770070.1"
                     /translation="MKLKDGFYSSSHGIGGLMLDMPTKNPKTREKPKFKVGDMVRCEA
                     EGFIYPFCGYVEHLYNHSAIIRIENTMECDKWLAKSKENLAVARLVDMEVI"
BASE COUNT          700 a          261 c          417 g          479 t
ORIGIN      
        1 tgaaaaaacc aaacggagaa caccgagaaa aattgcaatc tggcatggtg accgaattct
       61 agtaacaggt actgctgaac agttatctga aattactgga ttaagcaaaa acattatctg
      121 ggatagagct aaacgcgaga acgttgattc gaaaggtcgt caatttaaac attgggagaa
      181 gaaataatgg aggaactaat cacaaaagta gagcagtggg caaaagataa gggattggat
      241 caagctgatt ccagcaagca aatgttaaaa acgatcgaag agattgggga agttgccgct
      301 tctctagcta gaaaagatga atatggttta agagacggaa ttggagacgt agtagtaacc
      361 ttgattattt tagctatgca aaatgatatg gatttgtacg agtgtctgaa ccaagcatac
      421 aacgaaatca aaggacgcac aggaaaaatg gtagatggtg tattcgtgaa gtcgagtgat
      481 ttggaggaca gcgatgaata aaaaagtatt aattgataaa caagcattga ttgatgaatt
      541 aatgaaaata cctggtgtgg gatctaatag tgacgcttta gaaacgatta aacgtttccc
      601 atcgtatgaa ccgcagaagc cagttgtgcc taagtttgtg gcggaactac tcgactatta
      661 tcgtcagtca acagatgttg atttattagc tttattgata actttccatg attggtatta
      721 tcgcaaaact aaagacggtg agcatgaaga agcaattgat tggctagttg atcatcctga
      781 aatttatatg cgtgcatggc tgtttggcta cgagctcgag aaagagccgt tgtattgcgt
      841 aataatcaat ggtagatatc ttgtcaaagt attcagcaat acaaacgttg tcatattagt
      901 tccagcagat gaattttcac agtacgttac tcaaaaattt caattaactg aaaaacaaat
      961 caaagcaatt gatgaaagat actggccgtt tgctgtgcca gtggatgagg tggtagaagg
     1021 atgagcgacc catacattac taaattagaa aagttgtccg aagattttga gaagaataaa
     1081 aataaactaa ctaaagataa acttatagaa ctggttaggt tggttatcag aggagctatt
     1141 acaagagaaa aggcgcagca aaacatagaa atttttgctc gtagtgtccg agatggatta
     1201 gagaagaaag ttaaacaaaa tgaacaacag acaccgcaga ataacaaaac taagaaaacg
     1261 ggaactgaat gtactaaaga caaagtttga aaaagaatat ggaatttcag cagaagaaac
     1321 atataaagtg gcaagtcagt gtgttgctga tgcgagcggc gctattcgta agtttggaat
     1381 ttcgatatta agtgatgatc gtaaatggga ggaaaaagaa tgaaactaaa agacggattt
     1441 tactccagca gtcatggtat cggcggttta atgctagata tgccgacaaa gaaccctaaa
     1501 acacgtgaga aaccaaaatt caaagtcggt gacatggttc gctgtgaagc agaaggattc
     1561 atctatccat tttgtggata tgtagaacat ctctataatc actcagcaat cattcgtatt
     1621 gaaaacacga tggaatgtga caagtggtta gcgaaaagca aagagaattt agcagtagcg
     1681 agattggtgg atatggaggt tatataaaca gcgaaaaaat gtacttctaa gagaaaaatt
     1741 tattttttat aaaaatagga aagtatcatt atgaaaaaga ccacagagat aaataaacct
     1801 accacataaa aaatgaatat ttcttgcgat tcaatatagg tatatttaac aatttgt
//
LOCUS       DACDIE010000157         1833 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.161, whole genome shotgun sequence.
ACCESSION   DACDIE010000157 DACDIE010000000
VERSION     DACDIE010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.161"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(358..855)
                     /locus_tag="IV250_002848"
     CDS             complement(358..855)
                     /locus_tag="IV250_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP8770071.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(895..1704)
                     /locus_tag="IV250_002849"
     CDS             complement(895..1704)
                     /locus_tag="IV250_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8770072.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
BASE COUNT          532 a          339 c          315 g          647 t
ORIGIN      
        1 atcaagtaat aagaacagcc aagtaaaaac agcttcttat gtttttactt ggctgttctt
       61 attactgcag atgcaagctt ttgaataccg tttaaggaag aggttgtgaa atcagcgttt
      121 tgacctgagt attagcttaa caaacggaaa aatagttcct catattttgc cgtttgttaa
      181 gctaataccg agggtcagct gattgaacac catttaagaa agaggtcgtg agcctgccgt
      241 ttcactctgt gtcataaaaa agccaatcta ctttgataga aaaagcacgt aggactaaaa
      301 cagttctgcg tgcttttttt attgtgtgtt aaatatcgta aatgaagata gatattttta
      361 tgattcgacg atagagacgt tgaaatcttt caacagctct aaagcggatt ctccgtgttt
      421 tctactggag actaacattg gatcaacaaa aaagtctgct tcaggaaaag cactctgcag
      481 gcctatcaga ttagagagta cacacaagtt tgtttcaatc ccgactactt cgatctcttt
      541 ttgttcttct ttgctttcaa ataaagtttc ctggatttca gctaaagttt caggtggaat
      601 cgtaaagtaa tactttttaa tcactcgttc attttcttgt ggagaaagaa gagggatcaa
      661 ttgcaaatct tctcgttctt cttctttgtc cttcagttca actggtatat cccgtgtgaa
      721 gagaatatat tcattatttt ggcgtgcttt agctaatctt tgagcaatac gttctgccaa
      781 ttgatctgac tctggaatat aaaattcgct gtttggatca agaatatgat tttgcatatc
      841 aataataact aacattttct ttcctcctac attcatatcg tcattttatt ctttttaata
      901 gaagtttttt aaaatataag caacaccagc ttcttcattc gtcagtgtta cttggtttgc
      961 aatttctttt acttgtcgct gtgcattccc catcgcaata ctcaacccgc tgtaattcaa
     1021 catagcaatg tcgttttctt gatcgccaaa agccatgatg tcactcgatt cgattccaag
     1081 gtaatccgcc agaaaaacaa gtgaatgagc tttagtgata cctgtaggcg tgatttctaa
     1141 gaaatgcgga tcagagaata ctgattggat accatctgct tgcagatctc tttgtagctt
     1201 ctgtagtttt tcttcactct tatctgttaa tgctaattta agatagctaa gagaaggatc
     1261 ttccaacata tttaaaattt gatcaaaaga atggttctgt aaattgaaat gttcataata
     1321 agggtcatct ttgactagat ttgattcatc gaatgtcagc tgatcatctt gggtttctcg
     1381 gctagcaatg atggatattt tcgcttcttg agcatagaag aaggcttttc tcagtgtatc
     1441 gctatctatt tttgaacgtc tgagaacttt cgccattttt ccatccttca tttcagcaat
     1501 cagtgaacca ttggaagaaa tcaaatagcc accgattcct aattcggaaa aaatatcttt
     1561 agcagaaatg ctgttccttc cggtagcgac ggttacgatt ccgccatttt catcaaattt
     1621 ccgaagtgct tggatatttt cttctggtat ctttccattt cgatcaagca aggtaccgtc
     1681 taaatcaatc gctgctagtt tcattttatc ttcctacttt ctttttacat atctattttc
     1741 ctgttacctt aagattaaat gagaatttaa aaaaatgaaa ctaacttgct ttgtcattat
     1801 gaattttttc aaaaaacatt cttaagaaaa agg
//
LOCUS       DACDIE010000158         1801 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.162, whole genome shotgun sequence.
ACCESSION   DACDIE010000158 DACDIE010000000
VERSION     DACDIE010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.162"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..790
                     /locus_tag="IV250_002850"
     CDS             <1..790
                     /locus_tag="IV250_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770073.1"
                     /translation="SKTALDSLAKLFSSRFDSAEFVEYLSQNESGKTLIETMKKDYLN
                     ENDFPTWTNIEDATWDVVCTIYSPWVTIADFVLDKIGGDTYPDAKSLATSDFKTFYGY
                     LIGLIGNGIDLKVTPKQTPEEIEKEKGEVQMAYANDREKVKLLEGKLSEEELGKMVQE
                     INEDSNYFPELKVYQNDEDFFNEMFSNSPWEAVSKTNLGEWNFQDDLVAFSNDGYLRS
                     YSESEYRAELKTYETDILMSYLNLLESGQGELRDKVEELLKEEL"
     gene            878..1684
                     /locus_tag="IV250_002851"
     CDS             878..1684
                     /locus_tag="IV250_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770074.1"
                     /translation="MRGDQTMSATTDMALEAVIKDNTGNVENVEDFSDIAINLSAWIS
                     AVGEENIEFEIKNMDDLKAKFPFIASKLSQFAEDKSWNELVSFSEKIYDITQEDECVS
                     WEMNNQYKKIDAFVKSRIEGTAFEFEGIDTAENWLNKESFIKSIADDLEKQWSKEDYA
                     EYLKTERDTEVKEKLTEVCLDEHIDPNIVKQEDYEVVIDKVPTDFEEVVKYIVEEALS
                     QYSSDEEVDEETVRTRVWQLVFDDKDYKYLSDQNSVNWSVRLLIDQNDIE"
BASE COUNT          698 a          215 c          366 g          522 t
ORIGIN      
        1 atcaaaaaca gctttagact cattagctaa actatttagt agcaggtttg atagtgcaga
       61 atttgttgaa tatctaagcc aaaacgaaag tggaaaaact ctgatcgaaa caatgaaaaa
      121 agactatttg aatgaaaatg actttccgac atggacgaat attgaagatg ctacatggga
      181 tgtagtgtgt acgatatatt ctccttgggt aacgattgct gattttgttt tagataaaat
      241 tggaggggat acataccctg atgccaaatc tcttgctaca agtgacttta aaacgttcta
      301 tggttatcta ataggtctaa taggcaatgg aatagatttg aaagttacgc caaaacaaac
      361 acctgaagaa attgaaaaag aaaaaggaga ggttcaaatg gcttatgcaa atgacagaga
      421 aaaagtaaaa ttattggaag gtaaattatc tgaagaagaa cttggaaaaa tggtgcaaga
      481 aatcaatgaa gacagcaact attttcctga attgaaggta tatcaaaatg acgaagattt
      541 ctttaatgaa atgtttagta atagtccatg ggaagcagtg agtaaaacaa atttaggaga
      601 atggaacttt caagatgatc tagttgcatt tagcaatgat gggtatttac gatcatattc
      661 agaaagtgag tatagagctg aattaaaaac gtatgaaacg gatattctga tgagctattt
      721 aaatctatta gagagtggtc agggagagtt aagagataaa gttgaagaac tattaaaaga
      781 agagctatag ggattatttc aacataaaca caagcagaaa actaccaaac aaaacgtacc
      841 atgcaaaaag tcggttaagc cggctttttg ctctattatg agaggagatc aaaccatgag
      901 tgcaacaacc gatatggctt tagaagcagt aataaaagat aatacaggaa atgtcgaaaa
      961 tgtagaagac tttagcgata ttgcaatcaa tctttcagca tggatttcag ctgtaggaga
     1021 agaaaatatc gaatttgaga tcaagaatat ggatgactta aaagcaaaat ttccttttat
     1081 cgcttcgaaa ctaagtcaat ttgccgagga taaatcgtgg aatgaattag ttagtttttc
     1141 cgaaaaaatt tatgatatta cgcaagaaga tgagtgtgtt tcatgggaaa tgaataatca
     1201 atacaagaaa attgatgctt tcgtcaaatc acgtatagaa ggaacagctt ttgagtttga
     1261 aggtatagat accgcagaaa attggttaaa taaagaatcc tttattaaaa gtatagcgga
     1321 tgatcttgaa aaacaatggt ctaaggaaga ctatgcagag tacctaaaaa cagaaagaga
     1381 tacagaggtg aaagaaaaac tcactgaggt atgtttggat gaacatattg atcccaatat
     1441 cgtaaaacaa gaagactatg aagtggttat tgataaagtt cctactgatt ttgaggaagt
     1501 tgtaaagtat atagttgaag aagcattatc gcaatactct tcagatgagg aagtggacga
     1561 agaaactgtt cgtaccagag tttggcaact ggtttttgat gacaaagatt ataaatattt
     1621 gagtgatcaa aactcggtta attggagtgt acgattatta attgatcaaa atgatattga
     1681 ataaaataga gctgaaaagc tctatttttt tgataaaaaa actagctatt ttattaaaaa
     1741 taatttggtt caaaatttat ttttttaact tgataatttt ttgtcatcct tttttaaatt
     1801 g
//
LOCUS       DACDIE010000159         1796 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.163, whole genome shotgun sequence.
ACCESSION   DACDIE010000159 DACDIE010000000
VERSION     DACDIE010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.163"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(49..>1796)
                     /locus_tag="IV250_002852"
     CDS             complement(49..>1796)
                     /locus_tag="IV250_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP8770075.1"
                     /translation="ARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIK
                     SRTLQQGIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVD
                     TWDVTRLQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARG
                     KWREKDRPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGL
                     GDWHVNPKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRK
                     PNHGRNQYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQ
                     GEVFHRYVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIE
                     RLKIQYGTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSE
                     GDLRIVKEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTL
                     NEVNAPYRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPET
                     FDFDSKIWVITQEEK"
BASE COUNT          540 a          367 c          257 g          632 t
ORIGIN      
        1 tacttaagat tgctcctttt tgaagtaagc ttttttggtt attcatctct acttttcctc
       61 ctgtgttata acccaaatct tggaatcaaa atcaaatgtt tctgggcgtc gattctgatc
      121 atttatttgt ccactagatg catctgaaca gactagtcct gctctcatca attccattcc
      181 agatcgcaaa ctattttgtc ctacattata aagtccgtca ttatctaacc cttttaattt
      241 cagtcggcgg taaggagcat ttacctcatt taatgtttta taatgagcaa caatagccgt
      301 tttcttatca tgactaacaa ccatccacgc ggtttgatta tcatcatcga atggtgataa
      361 tagtctataa aaagtaccat tatgaattat ggttctatat tttttataaa aggcaatttg
      421 ttcttttaca atcctcaaat cgccttcact taattcattt aagtcaagct catatccaaa
      481 tgcaccaaaa aaagctacat ttcctctagt ttttataggt gttatacggt ttgtttgatg
      541 atttggaaca attgatacat gagcaccaat agacgaaaga ggatacagca tactagttcc
      601 atattgaatt tttaaccttt caattgcatc tgaatcatca ctagcccaag cttgaggtgc
      661 atagtgaagc attccaggat caaagcgccc accacctgat gaacaagact caaatagtat
      721 gttaggatat tttgttataa gtttttcata taagcgatat acgcctagta catatctgtg
      781 gaatacttct ccttgttgat ctgctgacaa gtgactgtca aatacatcag tcaatggacg
      841 attcatatcc cacttaatgt aatctaagtt tgattcatca atgattttac acatttgatc
      901 gaaaatattt tcaacaacct catctaaccc aaaatttaat acatattggt tcctaccatg
      961 attaggtttt cgattaggtg tagaaataat ccaattcgga tgtgtgcgat ataactcact
     1021 atccttattc accatttcag gttcaaacca taaaccaaat ttcataccaa aactacgaat
     1081 tttttctgaa aggctgccga ttcctctagg tagtctttta ggatttacat gccaatctcc
     1141 taatcctgca caatcgtttt ctctttctcc aaaccagcca tcatctaata caaataattc
     1201 aataccaaca tcaaatgctt ttttagcaat atttaccaac ttttcttcgt caaaatcaaa
     1261 atatgttgct tcccaattat taattaagac aggacggtct ttttctcgcc acttaccacg
     1321 agctaagcgt tttctaaata gttgatgaaa agtttgactc atggcattta aacccttact
     1381 tgaataaacc agaatcgctt ctggtgctgt aaatatttct cctggattga gtttccaatt
     1441 aaatccaaat ggattgatgc caacttgtag tctagtaaca tcccatgtat ctacttctgc
     1501 ttgaattaag aagtttcctg agtaaactag tgctgcacca atagcttctc cttgattttc
     1561 atcagtattt tttcttttta aagtaacaaa tgggttatgc tgatggcttg aaatacctcg
     1621 agtagattcg attgattgaa ttccttgttg taaagtacgc gacttaatag ttctttctct
     1681 accccaagca ccagataatt gttgccattc ataatttgca tcgggtaaat ccaatgataa
     1741 actgctaata ttctcgatta tttgaatatc ttttccttta ttcataactc gcgcgc
//
LOCUS       DACDIE010000160         1792 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.164, whole genome shotgun sequence.
ACCESSION   DACDIE010000160 DACDIE010000000
VERSION     DACDIE010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.164"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..173
                     /locus_tag="IV250_002853"
     CDS             <1..173
                     /locus_tag="IV250_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP8770076.1"
                     /translation="RPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSV
                     CTEIKVLLGIPA"
     gene            229..681
                     /locus_tag="IV250_002854"
                     /pseudo
     CDS             229..681
                     /locus_tag="IV250_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
     gene            926..>1792
                     /locus_tag="IV250_002855"
     CDS             926..>1792
                     /locus_tag="IV250_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HAP8770077.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNLVGFMR
                     ATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPNVKV
                     QMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLISYG
                     GLLKEIHKKLNLD"
BASE COUNT          693 a          229 c          360 g          510 t
ORIGIN      
        1 atcgtccagt aaagagacgc aataaattct atcgaagttt acgcactgcc tctaccacga
       61 ttaaaggcat ggaagccatt cgaggattat ataagaaaac ccgaaaagaa ggcactctct
      121 tcgggttttc ggtctgtact gaaatcaagg tattattggg aatcccagct taaatcatag
      181 ataccgtaag ggattttatt ctttatttaa aactttgcaa cagaacctaa aaacttagtg
      241 actgctgcga gagataacga taggttaaaa cagcatgtta gaaatctcat gagtactgat
      301 atggcgagag aatataaaaa attaagtaaa gaacatgggc aagttaaaga aaaatatagt
      361 ggtcttgtag agcgatttaa tgaaaatgta aatgattata atgagttgct tgaagaaaac
      421 aagtctttaa agtctaaaat aagcgattta aagcgtgatg tgagtttaat ctatgaaagc
      481 actaaggaat tccttaagga acgtacagac ggcttaaaag cctttaaaaa cgtttttaag
      541 gggtttgtag acaaggtaaa ggataaaaca gcacaattcc aagaaaaaca cgatttagaa
      601 cctaaaaaga acgaatttga actaactcat aaccgagagg taaaaaaaga acgaagtcga
      661 gatcagggaa tgagtttata aaataaaaaa agcacctgaa aaggtgtctt tttttgatgg
      721 ttttgaactt gttctttctt atcttgatac atatagaaat aacgtcattt ttattttagt
      781 tgctgaaagg tgcgttgaag tgttggtatg tatgtgtttt aaagtattga aaacccttaa
      841 aattggttgc acagaaaaac cccatctgtt aaagttataa gtgaccaaac aaataactaa
      901 atagatgggg gtttctttta atattatgtg tcctaatagt agcatttatt cagatgaaaa
      961 atcaagggtt ttagtggaca agacaaaaag tggaaaagtg agaccatgga gagaaaagaa
     1021 aatcgctaat gttgattact ttgaacttct gcatattctt gaatttaaaa aggctgaaag
     1081 agtaaaagat tgtgctgaaa tattagagta taaacaaaat cgtgaaacag gcgaaagaaa
     1141 gttgtatcga gtgtggtttt gtaaatccag gctttgtcca atgtgcaact ggaggagagc
     1201 aatgaaacat ggcattcagt cacaaaaggt tgttgctgaa gttattaaac aaaagccaac
     1261 agttcgttgg ttgtttctca cattaacagt taaaaatgtt tatgatggcg aagaattaaa
     1321 taagagtttg tcagatatgg ctcaaggatt tcgccgaatg atgcaatata aaaaaattaa
     1381 taaaaatctt gttggtttta tgcgtgcaac ggaagtgaca ataaataata aagataattc
     1441 ttataatcag cacatgcatg tattggtatg tgtggaacca acttatttta agaatacaga
     1501 aaactacgtg aatcaaaaac aatggattca attttggaaa aaggcaatga aattagacta
     1561 tgatccaaat gtaaaagttc aaatgattcg accgaaaaat aaatataaat cggatataca
     1621 atcggcaatt gacgaaactg caaaatatcc tgtaaaggat acggatttta tgaccgatga
     1681 tgaagaaaag aatttgaaac gtttgtctga tttggaggaa ggtttacacc gtaaaaggtt
     1741 aatctcctat ggtggtttgt taaaagaaat acataaaaaa ttaaaccttg at
//
LOCUS       DACDIE010000161         1752 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.165, whole genome shotgun sequence.
ACCESSION   DACDIE010000161 DACDIE010000000
VERSION     DACDIE010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.165"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            948..1421
                     /locus_tag="IV250_002856"
     CDS             948..1421
                     /locus_tag="IV250_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAP8770078.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1430..1657
                     /locus_tag="IV250_002857"
     CDS             1430..1657
                     /locus_tag="IV250_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8770079.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT          588 a          272 c          260 g          632 t
ORIGIN      
        1 tttcggagaa ttaaaaaata caataaataa ctttagttac tttaatgcgt tcttacttta
       61 tttttatatt atcatttttc ttattcactt ctttggttta tactattctt ataaataaaa
      121 acgaggtatg tcctatgatg aaaagattgt actattcatt gatcataacg attggctatt
      181 tgattgtatc aaatctagga aatatggtat ttggtatcag taaagaattc agttggacta
      241 ctacattatg ggaatctcta ttctttttca tcttcgtatt tttgttacaa aattatcgaa
      301 aaaaataaac atgtgccatc tatctgttgg cacatgtttt aggatttatg gtacttttta
      361 ttcggcaatc cattttattt ttctacttct tcttaatacc tctaacactt ttgatttata
      421 aactaatcca atgcatctac ttgcgcttat taagctattt cagctagata gtaccacccc
      481 atgcgtcaat gcttgatttt ccttttgtat aacattgtat aagcaataaa ggacaaataa
      541 gtggtgatta caccaaacca ttgaataaga aaaattctga tacgtaaata aacatgaact
      601 tgaaaaaatt aatgaatcgg ttagctacta cgtagttgtc ttttttctca tacagttctg
      661 ttaacttaca cttctcttcg ctagataata aaaaatcctc caactgtatt gtccaatata
      721 atcccaggca tctaaactag cattatagaa gagacacaca tagaatctaa aataactggt
      781 tttaaatgtt tcttacaatc aaaaaaagct gttaaatgca ctccacacct tcatactcta
      841 tcatttttta taaacgatat atttttattg tttttcgata aaaaatagtt gttttacaat
      901 aattattctg ctatatttta gctatcaaag ataaaagagg gataaaaatg aaatttaaaa
      961 cattgctatt aaaatttgtc gttttaggta taagtgccgt atttattttg ttcggcgtac
     1021 ttttttatac tcagatcgct acaagcgaaa aaggattcaa ttttgattgg cagaccacat
     1081 tatttgtttc tgtcatttac tttgcccttc tcctagggat cgttattgct tatttcttat
     1141 tgaaactatt gaaaaaaatc gaagataacc aagctttttc cactcaaagc ttaaacatac
     1201 taaaaaaaat cgaacgaacc attttgttta ttggtttcgc cttttttggc ttacttccta
     1261 aatttatgaa agtagcggat ttagacgatg ctccaggttt gatgttggta ggaatcgctg
     1321 tcgtattcat tccttttgca gtatacacat taacaagcgt gctggaaaaa ctgcttgaac
     1381 atgcgatttt acttaagaca gatcatgatt tgacagtgtg aggtgcgaca tggcaattat
     1441 cgttaattta gatgtagtat tagctcaaaa aaaaatgagt gtgactgaat tgagtgagaa
     1501 agtgggcatt acgatggcaa acatctctat tctaaaaaat ggaaaagcaa aagccatccg
     1561 tttttccact ctcgaaaaaa tctgccaagt tttggagtgt caaccaggtg atttgttgac
     1621 ctataaaaat gactcgaata ctataccaga agaataaaat aaagtagaac aaacagtaaa
     1681 taaaaaataa tctgaatgat agatgtgtga gacttttaga agttatttac gtctattatt
     1741 cagatttttt at
//
LOCUS       DACDIE010000162         1675 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.166, whole genome shotgun sequence.
ACCESSION   DACDIE010000162 DACDIE010000000
VERSION     DACDIE010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.166"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(421..720)
                     /locus_tag="IV250_002858"
     CDS             complement(421..720)
                     /locus_tag="IV250_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP8770080.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            1071..>1675
                     /locus_tag="IV250_002859"
     CDS             1071..>1675
                     /locus_tag="IV250_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP8770081.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFA"
BASE COUNT          569 a          265 c          216 g          625 t
ORIGIN      
        1 tcccccactc atttccaatc ttttaactaa aaaattgata gttattttac aaatatttat
       61 aaaaaataaa tatgccaaaa aactgttttt acaaggtttt tcctaatgta aattattttt
      121 tcgcatatat tcattttatt ttacttattg ctttcatttt ttcaatctat atttccaatt
      181 tttaacttac aaaaatgcaa cattaataaa taacgatagt ttcataaaat acacttttaa
      241 ttaacattat tagtttatac tagctgtttt tgtgttaatt aaaggataaa atacaatata
      301 aagaaacacc tgtttcttta tttagaataa aaaaatacag actctgttca aataaatgaa
      361 cctagcctgc attatgagat tgactatact ttttaattca ttgagatgaa taactcgttt
      421 ttataaatgc ccaaaagtaa tcggtgccga agccattcct cgacctaatt ctttttcaaa
      481 ttttcctatt gttgatatct ccatataaaa cggtgatact ggtttagaca attctcccct
      541 gattgctaac ggacgtaaac tacgttccaa attatttatg actttaggat aatactgacc
      601 tttttctaag cctgtctttg cgtctatcaa aagtatacgt tccttttctt tgatctctgg
      661 atttaaaacg agatcatata attttagtac catgttttct atttcttcac ttttagacat
      721 cacgatttcc tcccaaaaat atatttcttc ccactacatt ctataaacaa agtatacgct
      781 acatctagat ttttttaaat cattactgga ttgttaaaac ctttcaatac actgttatgg
      841 caacgatttt tattttaaaa acattctaaa ttaaatcagt aaaaattttt attctaagat
      901 ttttattaga attacgccta atttgtgtct ctcagggcaa ttctaagaac gtttcaagag
      961 atagcgacac tatcgttatc gaaaaaggcg aaaacctatc tcattttctg ctaatgtaag
     1021 ttggtttagc ttttgttaat atgactttat gaaaatggag gtaattaaat atgatttttc
     1081 ctaaagttaa tgaagcaagc ttgaataaag gatccttttt tcatatctat gaaaacgaag
     1141 attttgaact gtataatatc ctttcacaaa aagaggtcat cgaaaaccaa ataatctgcc
     1201 atccaattat ttacaaatct attttcaagt atgcattttt tgcttccaat gattttcaaa
     1261 gaatggagat taccgactgt atattcgaaa actgtgattt ttcaggctgt atattgacaa
     1321 aaagcatgtt gcatcgcgtg aaatttgtca attgtaaatt aacaggtact aaattgatgg
     1381 aaagttatat tgggaacacg ttattcgaga attgtaaaat ggactatgta aatttatctg
     1441 gttcaaacat aaaagaaagt acctttgagc actctgtctt atcaagcgca gattttgtag
     1501 actgttccct cactaaaata atgttttcta ctaatgattt agaaaatgct agcttttttg
     1561 atactgattt aaaaaatatc gatttaagta gcaactcctt tgaaatgatt gaagtacgct
     1621 cggaacatct aaaaaattgt acgcttaatc agttgcaagc acttggtttt gctag
//
LOCUS       DACDIE010000163         1604 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.167, whole genome shotgun sequence.
ACCESSION   DACDIE010000163 DACDIE010000000
VERSION     DACDIE010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.167"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..182
                     /locus_tag="IV250_002860"
     CDS             <1..182
                     /locus_tag="IV250_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP8770082.1"
                     /translation="ESFNKQIKRYSRRKEQFQNEESLERFLVSIFDTYNQKFLNRSHK
                     GFQQVTDTLVSMFTE"
     gene            519..758
                     /locus_tag="IV250_002861"
     CDS             519..758
                     /locus_tag="IV250_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770083.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(1120..1380)
                     /locus_tag="IV250_002862"
     CDS             complement(1120..1380)
                     /locus_tag="IV250_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770084.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
BASE COUNT          543 a          283 c          218 g          560 t
ORIGIN      
        1 tcgagtcttt caataagcaa attaaaagat acagccgtag aaaagagcag tttcaaaatg
       61 aagaatcact agaacgcttt ctagtcagca tttttgatac atacaatcaa aaatttctaa
      121 acagaagcca taaaggtttt caacaggtaa ccgatacatt agtttcaatg tttactgagt
      181 aactaattat tttgcaggag gacaatttat ttacacaaaa ttattgacgc tcccagctat
      241 cctcctagca actatttaaa tacccaaaaa aattacctct agataatatc atacctacgt
      301 tttcacttat aaattttttc ataaaaaaag cgtttagagg cataatcgtt aatacagatg
      361 gtaaccaatg tatctggttt gaactgggat atcaccctct ccatatatgt attactaaga
      421 ctaatagata ctataaaaga cttgatagtt ttgcattgat tttgcctaat actgagcata
      481 ggtgtatgct aaacgatact ataaggagtg tgtacataat gaaaagaatt ctgtttatca
      541 gtttatcaaa tttattgtta cttactgctt gtgggacttc tgcatcaaag aacatacctt
      601 cagatacgca aatttcgaat agtatcgatt caacaatttc tacaaatgag aaaaattctc
      661 cggtagaaac ttccgatata aaagtttcta cctctgcaaa aaacagggta aagaaactgt
      721 cagcacgtct cctttatttc aaacaaactc tgaattagct tcttcatctt cttcagcaaa
      781 taatgatgat agtttcgcgt ttactcctaa ctaaatgcaa cctttacaaa tgctttacga
      841 taacaagcct gagctaaatg atcctgatat cagttttgca ttttttgaaa tgattggatc
      901 ggatgatctt tttaaaacta ctagtttgtc tatccgaaaa taggacagtt ctggcagtat
      961 aggtttttat cgagtttctc caccaggcct cgttacagag acagattctc taggcgaacc
     1021 ttttgaataa atcaaaaatc atgaacaatc actgccaact aaataaagtg ccttcaaaag
     1081 cttggtcttc tcgcttttga aggcacttta tttaaatctt tatgaaacta aagtatgttc
     1141 atatttgttg ctatagttat atagatattc tgttgtttcc aactcataaa cgtgagtata
     1201 ttttgccact acttttccgc atttttctaa tatttcattg cgatcgacgt catgatattc
     1261 gtctacgcag atcacaacac aattttctaa tttactgatt acttctccaa ccactttttg
     1321 tttcaagcca ataggttggc aaatatattt ttttcctaaa atgatagttt cgttattcat
     1381 atttcatcca tccttaatgt ttttccatta tttaaaatga taataccatt aaaatatgat
     1441 tatgaaaatc aataacactt gtgattttat aaaaaaataa aaaaaccgaa aaacagaaat
     1501 gcccactgtc tttccggctc tttttattct tgatttctct tcttttttgt ttcaatagac
     1561 gattttagtt attaataaat gaaaaagcat ttatattaaa attt
//
LOCUS       DACDIE010000164         1516 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.168, whole genome shotgun sequence.
ACCESSION   DACDIE010000164 DACDIE010000000
VERSION     DACDIE010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.168"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            164..1108
                     /locus_tag="IV250_002863"
     CDS             164..1108
                     /locus_tag="IV250_002863"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP8770085.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
BASE COUNT          546 a          222 c          293 g          455 t
ORIGIN      
        1 taggtgtaaa catttgtcat ttatctgtta tgcgtattca ggaattcgtt tccatcaaaa
       61 attataataa caactattat aattaacatg ttgataaaat gaataaacaa gactatactt
      121 ttcatgaaag taacaaatag ttatgaaaag gagctgttta ttcatgaaaa aaacaagtcg
      181 taaagtagta atcgtcggta caggttttgt tggaacaagt atcgcttatg caatgatcaa
      241 ccaaggaatc tctaatgaat tagtattgat cgatgtgaat caagaaaaag cagaagggga
      301 agcattggac cttttagatg gtatggcttg gggcgatgaa aatgtggctg tatggtcagg
      361 tggctatgaa gaatgtaaag atgctgatat cgttgttatc acagctggta tcaaccaaaa
      421 acctggtcaa tctcgtttag atttagttaa aacaaatgcg tcaatcatgc gccaaatcgt
      481 aaaagaaatc atgggatcag gattcgacgg tattatcgtt gttgcttcaa acccagtaga
      541 tattttgaca tatatcgctt ggaacgaatc tggtctgcca acatcacgtg ttatcggtac
      601 tggaacaaca ttggatacaa ctcgtttccg taaagaaatc gcattgaaat taaaagttga
      661 cccacgcagc gtccacggct atatcttagg tgaacatggt gattcagaag ttgctgcatg
      721 gtcacataca actgtcggtg gtaaaccagt gtttgaaatt gtagaaaaag accatcgtat
      781 tgcccaagat gaattagatg tgattgctga taaagtccgt aatgctgctt atgagatcat
      841 tgatcgtaaa aaagcaactt actatggtat tggtatgagt actgcacgta tcgtaaaagc
      901 tatcttgaat aacgaacaag ctgtattgcc tgtatctgct tatttaactg gtgaatacga
      961 cgaaaaagat atctttacag gtgtgccatc aatcgttgac gaaaatggtg ttcgtgaagt
     1021 tgttgaatta tcaatcaacg aagaagaaaa agcaatgttc agcaaatcaa caagtgcttt
     1081 aagagaagta ttgaacactg tattataaga aacaatgaat atgaactaaa aatcctaaaa
     1141 ctgcttaaca gctgaaatag ctgttaagca gttttttatt gatatctaat aggtaagcaa
     1201 ataaacagaa tggttaaaat taatcggtcc gaaaatgtat ccgtacatat gaacatatga
     1261 aatttaatct gttgattaca ataagcgaat cgttatcaat gtttatctgt ggttaattaa
     1321 aaaatccttc cgcaacggca aatacaatga ttcatatcta attcgttcat atttgattaa
     1381 ctgaataaag aaactggttt ttatcattca attaaacgac aaaaaaagta agaaaaactg
     1441 caaaattttt ttataatttt gcataagcag attttgatgg aaaaatgacg atttttgata
     1501 aaaaaccgtt cgagag
//
LOCUS       DACDIE010000165         1474 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.169, whole genome shotgun sequence.
ACCESSION   DACDIE010000165 DACDIE010000000
VERSION     DACDIE010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1474
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.169"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            89..421
                     /locus_tag="IV250_002864"
     CDS             89..421
                     /locus_tag="IV250_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAP8770086.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            1040..1381
                     /locus_tag="IV250_002865"
     CDS             1040..1381
                     /locus_tag="IV250_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAP8770087.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT          534 a          190 c          324 g          426 t
ORIGIN      
        1 tgtcatgaac gaaaatcaat tctctatttt tgaaaaataa caagaaaaac ttgttaaaaa
       61 tatttaaaga aattgggtgg tttattttat gtcatataca ggaattttga gtctcaaaga
      121 tatttgtcac tacggtaaac ggtgtaccgc tacagagaaa atcacgaaga aactatcaac
      181 tggccaaagt aaaaccgttg tgcagtgtaa gaaatatata atccagaaag acaaagtctc
      241 agaggaaatg atttattaca ttgggaaaca aaagcaaata attttaaaag atcctatccc
      301 cttaaaagag ctatatccaa caatcaaaca tgtttatgac caaaacggtg tactgattgg
      361 tcgaagaaaa aatggagtct tgcgttgtac cgcaaaaggg atggggcgtt tgatcggctg
      421 aattgatcat tctcataact tgtattttat cgtttttcgc ttgcttgaaa atattgtcga
      481 gacttctaaa ggaacttaga gtcactagaa aaataaggag tagacttgat ctatcaacaa
      541 ttcccttcgg ggagtgacgg ggtaggtttt aacgattggt aacgtaaaaa attggtcaat
      601 cgagtaaatg cgcttgtttg gatagtggtg aaatgaattt gtgtaaataa taagcgcaga
      661 gtaaacaaga gggaatttag aagttagaca agttagaata gcgtctagat taatcatgag
      721 cagctactaa gggatctaga ctttgacgca aactcatgga ataaggagta ggtcttctcg
      781 taatggggag gacgcacaag aaattgtgtc gtgtgctgca gtacatgtag atacttgcga
      841 ggacgttgat tattggatca atcgagcatt taccgctgtc tactagggtt tctcgtttcg
      901 gacgccataa acgagtcgaa attttttatg tatttttaag taaacgaaaa acgataaaat
      961 atgaacaagt cctgaataaa gtgaaaaaat taatataaag catagagaaa atgaaattta
     1021 cgtaatggag gaatgacaaa tgaaaaatta tgatccgaat atacgattgg gcacacatac
     1081 tattaaggtt tctttccaac gttgggatta caaaggattt gtgacgttta gaaggggcgg
     1141 aaattgtaaa ggattagatg ttttagcatt agacgaggat gatttatacg atcaaacatt
     1201 gactgataat ccaattggat tcggcttgct acctgaagat gatgaaggta atgaatggtt
     1261 taaaatgacg cttatgaatg ataatggaga cgaattatct gttgaagata cttggtcata
     1321 tttgagtgat tatattgtca gctttgaaat cattgaattt gtagcggata aagaagaata
     1381 aaaactggag gaataaattt tacagataaa ggcttagaag gagtcacgac gattaataaa
     1441 aaggtagtat taccttaggc attgagtaag gaat
//
LOCUS       DACDIE010000166         1469 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.170, whole genome shotgun sequence.
ACCESSION   DACDIE010000166 DACDIE010000000
VERSION     DACDIE010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1469
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.170"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            279..>1469
                     /locus_tag="IV250_002866"
     CDS             279..>1469
                     /locus_tag="IV250_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAP8770088.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPN"
BASE COUNT          532 a          190 c          288 g          459 t
ORIGIN      
        1 taactgatat tttaattctg ttcatgccat ataatatcaa attttgaagt aatacactag
       61 ttataaaaat aaaagaatac atgaaaaaaa gaaaaaataa gtttcgataa aatcagtcca
      121 aaaagaagga gtattttatt tttctgaata aaaaatacaa tcattctgat atggtagaaa
      181 aaagcaagtt tattcccttt ttatattgac aacggttaca atgggtttta aaatataaaa
      241 tgtatcaaaa ctaacatatc agtttgagga ggttaattat ggtttcgatt aaaaattggc
      301 aaaattctat cgaagaattc aatgaaatgc agattctact gccgtgtttt gatgtcaaag
      361 aagttaagca agaaggccag caacatccag tatggcttca ttttggtgga ggaaatcttt
      421 atcgaggatt tcatgcagaa attgctcaat gcttagcaga tcaaaatgaa ttagaaacag
      481 gagtagttgt gtgtgaaaca tttgatgaag aagtagtgga aaaagcttat catgactata
      541 acaatgacat tttggaaatt gttatgagtg gagatggtga actcaaaaaa agacttttgg
      601 cagtaacggc tgatagttta tattgcaatc cgaagaatat agcggactat caaagagtgg
      661 ttaaatattt tgaagatcct aaattacagt ttacaacgtt tactattact gaaaaaggtt
      721 acagtttaaa agattctaaa gggcaattct tttcggtagt caaagctgat atcaaatctg
      781 gacctagcaa tcctcaacat acgatgagta ttgtaacatc cttgttatat agcagattta
      841 atgctggaga gttgccgatt gcgatggttt ctactgataa tttctcacag aatggtcaac
      901 gttttcaaga tagtattttt acgattgcag aagcttggca aaaagaagga tatgtttctg
      961 aaaaatttat tgcttattta aaggataaag aaaaagtgac ttttccttgg agtatgattg
     1021 atcgaattac gccaaatcct tcagagaatg taaaggaaga tctattgatg atggggctcg
     1081 aggatttaga tatcattcac acaaaaaaac ataccaatat tgctgctttc actaatactg
     1141 aggtagttca ttatttagtt attgaagata gttttccaaa tggccggcct aaattggaaa
     1201 aggctggtgt gattatgaca gatagagaga cggttgataa agcagacatc atgaaggtta
     1261 cgacaagctt gaatccactt catactgctt tggcagtaac aggttgtctg ctaggatatc
     1321 aatcaatttc agcagagatg caggatgatg atttggttgg tttaatcaaa ggaataggtt
     1381 atttagaagg tttaccagta gttgaaaatc caggcattat tgatccaaaa caatttattg
     1441 atgaagtaat taataaacgt ttgcctaat
//
LOCUS       DACDIE010000167         1456 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.171, whole genome shotgun sequence.
ACCESSION   DACDIE010000167 DACDIE010000000
VERSION     DACDIE010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.171"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(100..1119)
                     /locus_tag="IV250_002867"
     CDS             complement(100..1119)
                     /locus_tag="IV250_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP8770089.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(1130..1336)
                     /locus_tag="IV250_002868"
     CDS             complement(1130..1336)
                     /locus_tag="IV250_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP8770090.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
BASE COUNT          403 a          303 c          246 g          504 t
ORIGIN      
        1 tgggtgatag tgaagatatg cgataacatc actattttat cgaatgattt ttatagttgt
       61 caatatagtt atgtaatgct cctcaacgag gagctatttt tatcgtttag gaatatttaa
      121 ataccaacgt ttgtcatgga aatcttgcgc accgccttta gtgtttccct ctggatcatt
      181 cgttgcccgc atcatgacat agactttctt attagggaag ttacgcatgt taaaagatac
      241 atgatagcca acatttccag aagtattata agcttgattt acatctgatc tataaattcc
      301 atcagctctt actcgagcta attctttccc agtattgtaa tccataatga agatatactc
      361 gtatttatag ttagcaatgt gccatccagc tacatgcaaa tttgcatttt cgatttctcc
      421 aaactgatca atgtgggcgt aattcgttcc atctgtcagt gtaggattag ctgcaccagc
      481 tcgtgttgga tcaatgacag gcttgttttc agaagttgtt ggattttcat cggtaaatcc
      541 atgagctaaa tcatatgcta atttttcttt acttacgccc atttcagaaa gataaccgta
      601 aggatctgta tgatcgcccc agatgttttg tgttacccat aaatgcgatt tgattcccgg
      661 ttggttataa ggagtgtcca acgttaatgg aataccatat ttcattgctg aatctctagc
      721 caattcaacg tatgccttgt agtttttctc aaacgttgct ttatcatgtg tgtgttgtaa
      781 ctcaatctgc acaggactgt tggcattagc atacgaacca gcaccgtact gtacataacc
      841 aggttgaccg acttgataaa caattccgcc gtctcccaca atataagcag tataagcgct
      901 agtccatgaa cgttgcatat actgcgcttc attgcgtcct gttgctgttt cattagccgt
      961 ttcatgcagt aaaatatact gattatttgc tacttgtgag ctaccttcat ttgcgcccaa
     1021 attaaattca ttgttgatag tataggcaaa tccgttaatt ggcaataaaa aaagagccgt
     1081 taacaggctc atcgcagtaa tagtaatttt ctttttcata tctatcctcc tatttatctg
     1141 atgaaatttt cttatacgtt cgatttgata cacctaaaat actccctaga aatgctccaa
     1201 aacctgtaat aatcatcacc gttatatctg tgtaccgcca gttaacacct tttccgacaa
     1261 gaccgacaaa agtcgctaaa gcggggataa ctactatagc gatccatttc aaaatatcaa
     1321 aggttctatt gttcatacta tccacctccc ttcattggtt gggctacttt ctttttcaat
     1381 cgcttgtttt cttctatcaa tatttctctt tctgaaagca aggcatcaat ttgcccgtcc
     1441 agttgttcaa ttttat
//
LOCUS       DACDIE010000168         1450 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.172, whole genome shotgun sequence.
ACCESSION   DACDIE010000168 DACDIE010000000
VERSION     DACDIE010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1450
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.172"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..785
                     /locus_tag="IV250_002869"
     CDS             <1..785
                     /locus_tag="IV250_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP8770091.1"
                     /translation="FLTKSEKKVADFILNSGKTIIYSTMSDIKKNTKVGDATIIRFCQ
                     KIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYSTIRLLDEEKLNEA
                     IKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQAQVASLLTVNDLV
                     IIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQTAIEEFLNGGSL
                     AGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            1015..1245
                     /locus_tag="IV250_002870"
     CDS             1015..1245
                     /locus_tag="IV250_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HAP8770092.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
BASE COUNT          500 a          210 c          231 g          509 t
ORIGIN      
        1 catttttaac gaagtctgaa aaaaaagtcg ctgattttat tttaaattct gggaaaacta
       61 ttatttatag tacgatgagt gatattaaga aaaacacaaa agttggcgat gctactatta
      121 ttcgcttttg tcaaaaaatt ggtttttctg gtttttctga tttaaaaatt gaaattgcaa
      181 aggaagactt tagtcaaaaa aaagaacatc cgactagtgt aaagtactat gatgaaattg
      241 ctaatagttt aacagaagct ctctattcca ctattaggct tttagacgaa gaaaaattga
      301 atgaagccat taaaatgatc actcaatcaa aaaacatata tatatttgga gtgggatcta
      361 gtggaaatac tagtcttgat ttagaaaata tgtttcttcg cgtaggagtt caagcaaaag
      421 ctgtactaga ccctcatttt caagcccaag tcgcttcttt attaacagtt aatgatttag
      481 taatcatttt ttctttatct gggaaaacga aagatactta tgattcattg aaaatagcta
      541 aaaaaaatgg cgcaaaaatt attgctataa ctaactatat tcattcacct ataggaaagt
      601 ctgcagactt agttttacaa acagccattg aagaatttct taatggtgga tctttagctg
      661 gaaaaatatc acaactttat atttgtgatc tattagttca tggttatgaa caaaacaata
      721 atattgattc agttgaatta agagaaaaag ttttgcgctc tattattgat aaaagtattg
      781 agtgaactac ccccacttag ctaaacctaa cggtttttaa cgattgaagt aggcaattct
      841 tgggatgaga gcatacttga tagcttattt tttactaata gcggtggctc ttattgacca
      901 aatatccctg tagttcctac ggttcttgat tttatctaaa gatattttta tcgtggaagc
      961 tattgatcaa cttgaaagaa aaacgtgtag ctcattatca ctagtttatc gagtatggat
     1021 gaagcaatgg atcatcctct cttaaataaa tttattaagg atctgatcct tcaaattttt
     1081 tccatgatcg atgagcagga acctactgaa tctaaacgac atcgggcaca aggaattatt
     1141 aagattgcta agcgaatgtt ttctataaag ggtgttctaa gctatacagt gttaattcaa
     1201 aagattccca atcatcttgt ttataagaac aacgttgaag attaaaaaag tgtttcaatg
     1261 ctaaaattgc aaaatacttc aatgttatga gacaaactgt ctataagaat caatttccac
     1321 ccatttcatc acttgttaaa agtttatgat ccttttgatt tttatattaa tttatctgta
     1381 tttaatacag attttattta ttagttcatc tgcctattgt attttatttt gttgccttac
     1441 catctgaagt
//
LOCUS       DACDIE010000169         1426 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.173, whole genome shotgun sequence.
ACCESSION   DACDIE010000169 DACDIE010000000
VERSION     DACDIE010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1426
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.173"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..389
                     /locus_tag="IV250_002871"
     CDS             <1..389
                     /locus_tag="IV250_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP8770093.1"
                     /translation="GMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQFIFELIFI
                     SIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQSYLILIS
                     IIVLSVVFASSLMLIKKPKEILSKIS"
     gene            399..1055
                     /locus_tag="IV250_002872"
     CDS             399..1055
                     /locus_tag="IV250_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8770094.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            1055..>1426
                     /locus_tag="IV250_002873"
     CDS             1055..>1426
                     /locus_tag="IV250_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770095.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNF"
BASE COUNT          578 a          147 c          240 g          461 t
ORIGIN      
        1 gtgggatggt tgttctttca ttaatcttga ttctatggtt aagggaaaga atttatgaaa
       61 taggtatatt cttatctatt ggaacatcta agatacaaat tataatgcaa tttatattcg
      121 agttaatatt catatcgata ccaagtataa tatcttcctt atttttagga aatgtattac
      181 taaaagtaat tgtagatgga ttcattaact cagaggattc aatgatttcc ggtggaagtt
      241 taataaataa tagtagtttt atgtcaaata taacaacact tggacaaagt tatttaatat
      301 taataagtat tattgtttta tcagttgtat ttgcttcttc attaatgtta atcaagaagc
      361 ctaaagaaat cttatcaaaa ataagttagg agaaaataat ggatatatta gaaataaaga
      421 atgtagcata tagttatgca aattctaaag aaaaagtttt gtcaggagta aatcaaaaat
      481 ttgaacttgg gaagttttat gcgatagtag gaaagtcagg aacaggaaaa tctacacttc
      541 tttccttact tgctggactt gataaacctc aaacaggaaa aatattgttt aagaatgaag
      601 atatacaaaa taaaggatat agtaatcata gaaaaaataa tatatcttta gtatttcaaa
      661 actataattt aatagattat ttatcaccaa ttgaaaatat tagattagta aataaatcag
      721 cagatgaaag tatcttgttt gaactaggtt tagataaaaa acaaataaaa agaaatgtta
      781 tgaaattatc tggtggacag caacaaagag tagctattgc tagagcattg gtatcagatg
      841 ctccaataat actagctgat gagcctactg gtaatctaga cagtgttact gctggagaaa
      901 taattaatat attaaagaca ttagctaaag atagaaataa atgtgtaata gttgtaacac
      961 atagtaagga agtagcagat tctgcggata tcattttaga actaagtggt aaaaagttga
     1021 aaaaagtaaa taaaatgaat ttggaggttg aataatgata aaaaatgcat ttgcttatgt
     1081 aactagaaaa agtttaaaat cattaattat catattagtt attttatcta tgtcaacttt
     1141 aagtctcatt agtttatcta ttaaagatgc tacggacaga gcttcaaaag aaacatttgc
     1201 taatataaca aatagttttt ctatggagat aaatagacaa gtaaatccag gaacacctag
     1261 aggtggggga aatgtaaaag gtgaagatat taaaaagata tctcaaacag atagtataga
     1321 ctcctatgta aaaagaataa acagtgttgc agatttagtt gattatgata ttattgaaac
     1381 tcaagagact cttgcgaatc aatcacctga aagagcaaag aatttt
//
LOCUS       DACDIE010000170         1425 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.174, whole genome shotgun sequence.
ACCESSION   DACDIE010000170 DACDIE010000000
VERSION     DACDIE010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.174"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(151..1338)
                     /locus_tag="IV250_002874"
     CDS             complement(151..1338)
                     /locus_tag="IV250_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HAP8770096.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          414 a          309 c          250 g          452 t
ORIGIN      
        1 gtaaaagttc aacctcatca aaagcacaat gttttgcgtt gtgaacctaa ctgccatgcc
       61 tttctttctg aggagagagg tcaggagcag tagcgcagcg actcttgatg tctctcctca
      121 gaaagaagag actcgcacag gttcatggtg ctaaatgtta ttcgaattaa attctaaata
      181 ttttcgtgga gaagccagcc actcagtgtc tttttccatc aaaagtgttc caattatccg
      241 agtgatggaa ttgatatttg gaaaaatccg aatcactcgt tctctccggc gaatctcttc
      301 gttcaaccgt tccaacacat tggttgtacg aatccttcgg cggatatttt caggaaaaga
      361 caaaatttga atagcatctt caaagccgtt ttctaaaatg tcgcaagcag cacttaattt
      421 tgaatcacaa ccatattttt ccaagaagtg ctcttttctt tcacgtgtca attcaagttc
      481 agaggcttta aagatactct tcaactcgtc ttttacatca gaagaaactt tctttggcag
      541 cttatcaaaa atatttctca aaaagtgggc ttggcatctt tgccagctcg cacctaaaaa
      601 gttcttgcga acagccttaa ctaacccttt gtgtgcatca gaaatgacca gttttacacc
      661 gaacaaacct cgttgaacga acgattgaaa aacggtatcc caattatctt ccgattcgcc
      721 gtcttgaaca tcaaaaccaa gaatcgtacg tcgtccatca gaatcaatgc cgataccaat
      781 atatacacct tttgagacaa tacgatgatt ttcccttact ttcatataca gggcatcaca
      841 catgaaaaat ggatattccg agccttctag gctccggttt ctccactcaa aaacaaatgg
      901 atccaactgt ttcatcagat ttgaaacgaa tgatttagaa acagtcgcac catccgttag
      961 cagttcaact gttttagtta ctttgcgagt ggagacacca gaaatgacca tttcaatcat
     1021 ggcagcgatc aaagcttttt cgcttcgctg atatctttca aagatagtag gactaaactt
     1081 accgtctctg gttcgtggaa catgaagttc taacgtacca attcgagttg taaacgaacg
     1141 atcgtagtaa ccatttcttt gtgattgccg ttcttctgaa cgttcatagg catttgcctg
     1201 aatatattga tttcgttgtt cttccatcag ctgattaaac atgatagtca taagtgtttt
     1261 tgcggtatca ttcgcaccgc tttcgtttat aattgcttga atttcttcgc tttcaaaagt
     1321 aaaatgtact tgagtcattg atatcagtcc tattcgagta tgttttctgc aaaaaacatt
     1381 gtactcctaa ctgagatcaa tggctttttt gatgcttcac tttta
//
LOCUS       DACDIE010000171         1418 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.175, whole genome shotgun sequence.
ACCESSION   DACDIE010000171 DACDIE010000000
VERSION     DACDIE010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1418
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.175"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1124
                     /locus_tag="IV250_002875"
     CDS             <1..1124
                     /locus_tag="IV250_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP8770097.1"
                     /translation="DLDELFQDHLERAINSLLQAELTAFLDYEKYDRNGFNSGNSRNG
                     NYSRTFKTEYGELNLTIPRDRNGEFSQQTLPAYKRTNDSLETTIIQLFQKGITMAEIS
                     KLIEKMYGHHYTPQTISNMSKLVAEDVLAFKERTLEANYSVIFMDATHIPVKRQTVSK
                     EAVYITIGIRLDGTKEVLGFTIAPTESAYIWKEVLQDLRKRGLEEVLLVVTDGLSCIE
                     ESIHSVYPNAQFQQCCVHVSRNIAHKVRVRGRKEICEDFKLVYQANSKEEALDHIDFM
                     TRKWKKQYPRVVNLLLNPALLTFYNFPHAIRRTIYSTNLIEGFNKQLKRYTRRKEQFP
                     NEESLERFFISQFNQYNQKFLGRIHKGFKEIQDTLESMI"
BASE COUNT          491 a          234 c          240 g          453 t
ORIGIN      
        1 gggatttaga tgaattattt caggatcatt tagagcgagc gattaattca cttctacaag
       61 cagagctaac tgctttttta gattacgaaa aatatgatcg aaatggattc aattcaggaa
      121 attcccgtaa cgggaattat tcacgtacat ttaaaacaga atatggcgag ttgaatttaa
      181 ctattcctag agatcggaat ggcgaatttt ctcaacaaac actaccagca tataagcgaa
      241 ccaatgattc attagaaacg accattatcc aattatttca aaaaggcatt actatggctg
      301 aaatctccaa attaattgaa aaaatgtatg gtcaccacta tacgccacaa acaatctcca
      361 acatgagtaa attggtggct gaagatgttt tagcttttaa agaaagaacg ttagaagcca
      421 attattccgt tatatttatg gatgcgacgc atatccctgt gaagcgacag actgtttcga
      481 aggaagcagt gtatattacg attggtattc gtttggatgg aaccaaagaa gttcttgggt
      541 ttactattgc cccaactgag tctgcttata tttggaaaga agttcttcaa gatcttagaa
      601 aacgtgggtt agaagaagtt ttattagtag tgacagacgg attaagctgt attgaagaaa
      661 gtatccatag tgtgtatccg aatgcccaat ttcaacaatg ttgtgtgcat gtatctagaa
      721 atatcgctca taaagttcgt gttcgaggtc gaaaagaaat ttgtgaggat ttcaaattgg
      781 tttaccaagc gaattcaaaa gaagaggcat tggatcacat cgactttatg actaggaaat
      841 ggaaaaagca gtatccaaga gtcgtcaatt tactcttgaa tcctgcccta ttaacctttt
      901 ataatttccc tcacgccatc agacgaacaa tttattcgac gaacctgatt gaaggcttta
      961 ataagcagct aaaacgatat actcgaagaa aagaacaatt ccctaatgaa gaatctctag
     1021 agagattctt catttctcaa tttaatcaat ataaccaaaa atttttaggt agaattcaca
     1081 agggatttaa agaaattcag gatacattag agtcgatgat ttaactgtaa ttaacagaat
     1141 ggattttcca tttacatata attcttgacg ctaccataat ctaaataacc atcatattca
     1201 tctactactt atataattga gttttattaa actttttaag tttctattct atcaaatatt
     1261 taaacaatct ctgcatccga aaagactacc tcgtatagtt ttactaacaa tatttttatt
     1321 agcgtgttca aatcctgatt tcttgtatca attattttct gttcactttc aataagaaaa
     1381 aagtctttac ttccatggat aataataagg gttctgtt
//
LOCUS       DACDIE010000172         1400 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.176, whole genome shotgun sequence.
ACCESSION   DACDIE010000172 DACDIE010000000
VERSION     DACDIE010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.176"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            15..425
                     /locus_tag="IV250_002876"
     CDS             15..425
                     /locus_tag="IV250_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP8770098.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            439..795
                     /locus_tag="IV250_002877"
     CDS             439..795
                     /locus_tag="IV250_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAP8770099.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          514 a          209 c          263 g          414 t
ORIGIN      
        1 aaaggagtga gaaaatgata aaagtatata cttcgcctag ctgtacttcc tgtagaaaag
       61 caagagcatg gttgcaagcg aatcaacttg aatttgaaga gaagaatatt tttgccgaac
      121 cgttaacaga aagtgagatc aaaaagatct tggcagcgac tgaaggagga gttcaagata
      181 ttatttccac tcggtcgaaa atttacgaaa agctgggcat agattttaac gaattaactt
      241 taaagcaaat gatcgtctta ttcaaagaat atccttcatt attgagacga cctattttga
      301 ttgatgaaaa aagaatacta atcggattta atgaagatga gatccggtca tttgtccctc
      361 gtgaaattcg atgtgttgcc agaaatctga tctatttaca aaacaataac atcttaaatt
      421 catagggagg gagccttcgt ggaagtgaaa gtctatggat cagcgcactg tgtaacaagt
      481 ctgaaggcga aacaatggtt agaacaccat caattgcctt atcaatttgt tgacttagaa
      541 gaacgcgatc taagtacaga agaagcagaa gaattatgca gttttgaaga agtgcaagca
      601 gaacagctat ttgcgacttg gtctgaggct tttcacaaca ttgcgattga cctttcctgt
      661 gtaggaaaaa aacaactgat ttttttatgc acacaacata ccaatctcct cagacgtcct
      721 ttgattatta tcgatgaagt tctatttatt ggatacaatg agcagttgat ggaacagctt
      781 atcttaaata aatagacttt aactgtttta atcaagtcac tgagcatttc atacagttgt
      841 aacagtcgaa cacttagcat caagcgtgtt atgtgttctt tttttgactt tatttttcta
      901 ctatagagac atgctcaatt tatgtggtca aaattcaaat ctaggtgggg atatagagtt
      961 aaaaatatat tgacatgtgt aaatttacat gatatattca tttctgttgt tttaagtagc
     1021 aaagcaataa aatagtattt taaaaaacat ataaaatagt tgttgacatg ttagaaatga
     1081 catgataaga taactgagtt gttaaaaaag aaccacttgt gaagcttgat tctatcgaaa
     1141 aaaacttcaa aaaagttctt gacaaataac aaatgataag ttatgataaa tgagttgctg
     1201 taagatttcg aaaaacgaaa tcaaaaaaac ttttaaaaaa gagttgacaa ttacttgtct
     1261 ctctgatatg atataaaagt tgctacggca gccaaaaacg aatcggggaa cactcctgat
     1321 gaagtagacc tttgaaaact gaacaaagta agacaaacca aatgtgtagg gcgtcttgat
     1381 tcaattcaag acaacaaaca
//
LOCUS       DACDIE010000173         1392 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.177, whole genome shotgun sequence.
ACCESSION   DACDIE010000173 DACDIE010000000
VERSION     DACDIE010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1392
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.177"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..544
                     /locus_tag="IV250_002878"
     CDS             <1..544
                     /locus_tag="IV250_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP8770100.1"
                     /translation="KQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKII
                     KKLNMPSEQYGIAVHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEH
                     DCRINNYVHLSPGVCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPS
                     HSLAYGNPAKIKKEGINFEF"
     gene            495..>1392
                     /locus_tag="IV250_002879"
     CDS             495..>1392
                     /locus_tag="IV250_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAP8770101.1"
                     /translation="MAIQLKLRKKELILNSRILLSSPHMGGTEEKYVAKAFKDNWIAP
                     LGENVDKFEESITKYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATV
                     NPILYQGAVPVFIDSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYER
                     ILAIANQFNVPVIEDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSD
                     KKYVEHSRFLATQARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIF
                     NRYKEAFSGIDRIEMMPEVDGMV"
BASE COUNT          484 a          194 c          302 g          412 t
ORIGIN      
        1 taagcaaaat aaaatgcaag atctgaaata tcttgggaaa ataaacgaat tgaatagctg
       61 ggcggatagc aaaaaaaaaa tctataaatt tattgtagct attggtgact gtgtagtaag
      121 aaaaaagatt ataaaaaaat tgaatatgcc aagtgagcaa tatggaatag ctgttcatcc
      181 aagtgctata gtggcaaaag aaatttcaat tggttatgga actgttattt ttgcgaatgc
      241 tgtaattaac ccagatgctg taattggaga gcatgctata ataaacacag gtagcattgt
      301 tgagcatgat tgtagaatta ataactatgt tcatttatct ccaggtgtat gcttagcggg
      361 tggagtacat gtaggtgtag gaactcaaat aggaattggt tctcaatgta tccagatgaa
      421 gaaaattgga tcttggtgta tgataggcgc agggagcact attgttaagg atattccctc
      481 acattcctta gcttatggca atccagctaa aattaagaaa gaaggaatta attttgaatt
      541 ctagaatttt attatcaagt cctcatatgg gaggaacaga agaaaaatat gttgctaagg
      601 cttttaaaga taattggata gcgccattag gtgaaaatgt ggataaattt gaagaaagta
      661 ttacaaaata tactggttct aagggcggag cttctgcgtt aagttcaggt acagctgcca
      721 tacatcttgc cttaagactg cttaatgttg gaaaaaacga tattgtcttt gctccttcat
      781 ttacttttat agctacagta aatcctattt tatatcaagg ggctgtacca gtgttcattg
      841 actcagaacc taatacatgg aatatgagcc ctgttgcact agagagcgca ttagaagaag
      901 ctgaaagaaa caaccaatta cctaaggcaa tcatcgtagt gaatttatat ggtcaaatgg
      961 ctgattatga acgaatttta gctattgcaa accaatttaa tgttccagtg atagaagatg
     1021 cagcggaatc gttaggttct acttataagg gaatttcgtc aggcttattt gggacaattg
     1081 ggatctattc atttaacggt aataagatca tcacaacctc aggtggagga gctctagtgt
     1141 ctagtgataa aaaatatgtt gagcattcaa gatttttagc aacacaagca agagatcaag
     1201 caccgtatta tcaacatagc gaaataggtt ataattaccg tctaagcaat atttctgccg
     1261 gtatcggacg tggacaaatg gaagtattga atgatagagt agctaaacga agagaaattt
     1321 tcaatcgata taaggaagct ttttctggaa ttgatagaat tgaaatgatg ccagaagtgg
     1381 atggcatggt gc
//
LOCUS       DACDIE010000174         1391 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.178, whole genome shotgun sequence.
ACCESSION   DACDIE010000174 DACDIE010000000
VERSION     DACDIE010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1391
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.178"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1259
                     /locus_tag="IV250_002880"
     CDS             <1..1259
                     /locus_tag="IV250_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8770102.1"
                     /translation="VIEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIGGT
                     VDLTDNVTNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEV
                     TDNRSNADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKK
                     GTIVVTYPDGSSDEVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDL
                     PKGTTYTWKEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITE
                     GDQIVTGKTEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGN
                     TSEPTNVVVSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNI
                     FTISGGLILLGTLGLLGYKNRKKENE"
BASE COUNT          554 a          203 c          301 g          333 t
ORIGIN      
        1 gtgtcattga agtaacgtat ccagacggta cgaaagatac ggtgaaagtt ccagtagaag
       61 tgacagacaa tcgttctgac gccgataaat atgaaccaac agtagaaggc gaaaaagtag
      121 aaattggagg cacagtagat ttaacagata atgttactaa cttgccagcg ttaccagaag
      181 gaacaacagt aacagatgtt actcccggcg gtacaattga taccaacaca ccaggcaatt
      241 atgaaggtgt cattgaagta acgtatccag acggtacgaa agatacggtg aaagttccag
      301 tagaagtgac agacaatcgt tctaatgcag ataaatacaa tcctgaagga caaaaagtga
      361 ctacagactt aaataaagag ccagatgcat ctgagggaat taagaacaaa gaagatttac
      421 caaaaggaac tacttatgct tggaaagaga aagtagatgt tagcacagct ggaaataaaa
      481 aaggtactat tgtagtgaca tatccagatg gatcatctga tgaagttgaa gtagacgtta
      541 cagtgacaga tactcgttcc gatgcagata aatataatcc tgaaggacaa aaagtgacta
      601 cagacttaaa taaagagcca gatgcatctg agggaattaa gaacaaagaa gatctaccaa
      661 aaggaactac ttatacttgg aaagaaaaag tagatgttag tacagctgga aacaaaaaag
      721 gtactgtagt agtaacatat ccagatggat ctaaagaaga agtagaagtt actatttctg
      781 tagtagataa aaaagcaccg aataaacctc aagttgatcc tattacagag ggtgaccaaa
      841 ttgttactgg taaaactgaa ccaaatgcag aggtgacagt tacattacca gacggaagtc
      901 aacaccatgg gacagctgat aaaaatggta attttacagt taaagttcct aaattagagg
      961 cagggacaaa agtgatagta actgcaactg atgaatctgg taatactagt gaacctacta
     1021 atgtagttgt ttcttccaac aaaaaagaca gtggaaaaaa cggtagtaaa ggtagtaaga
     1081 ctgacaatca aggtagcaac agcaatcaag acaaaaatag aggcaaaagc cagtcctcaa
     1141 aagtttttcc taagacggga gagagtgaca gtaatatctt tactattagc ggtggtttaa
     1201 tactgttagg aacgttagga ttattagggt ataaaaatcg aaaaaaagag aatgaataga
     1261 aaatcaattt cttaagtctt ctctgagaag aggaagaatc tttttcttcc tctttttgct
     1321 tttttatgat gggaaataag tgttagttaa agatgaatat actttttata gaaaggaaaa
     1381 gaatgtaatt a
//
LOCUS       DACDIE010000175         1368 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.179, whole genome shotgun sequence.
ACCESSION   DACDIE010000175 DACDIE010000000
VERSION     DACDIE010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.179"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            82..>1368
                     /locus_tag="IV250_002881"
     CDS             82..>1368
                     /locus_tag="IV250_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HAP8770103.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQLIN"
BASE COUNT          467 a          263 c          257 g          381 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat taatcaat
//
LOCUS       DACDIE010000176         1364 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.180, whole genome shotgun sequence.
ACCESSION   DACDIE010000176 DACDIE010000000
VERSION     DACDIE010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.180"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(140..1288)
                     /gene="tnpB"
                     /locus_tag="IV250_002882"
     CDS             complement(140..1288)
                     /gene="tnpB"
                     /locus_tag="IV250_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAP8770104.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
BASE COUNT          393 a          242 c          216 g          513 t
ORIGIN      
        1 taaacctgac ggttcttaac gcttgaagtg ggagcttctt gggaatagag catacttgat
       61 agcgtatttc tttactaaca gcggtgtctc ttatttacca agctatcccc gtagttccta
      121 cggttcttga tgattggatc taagccaatc ccatagaatg tagtcttaga ccttcggtca
      181 gaatattgat actagcattg atatcacgat catgatgagt atgacaaaca ggacaagtcc
      241 attctcgaat ttcaagagat ttcttgccat ctttatgtcc gcactctgaa caaatctgac
      301 tagacggaaa ccatttatct actttgatga tttttcgtcc ataccagtca gccttgtatt
      361 gtaatttagt cacaaacttt gaccaagaaa catcagaaat acttttagct aatttatgat
      421 ttcgcaacat accttttgtg tttaagtctt caatacagat aatatcgtga tttttgatca
      481 tttctgtact caacttattc agaaaatcag tacgttgatt cattactttt tcatgcaatc
      541 tagctacttt gcgtttttgt ttttgataat ttttagcttc aaatagattg gtacctttat
      601 ttttagccaa taaagcacgc ctagacaatt tgcgttgttc acgctttagt ttcttttcca
      661 ttttggacgt gaatttatta ttatcaattt tttgtccatc agaaagaatc gcaaagtcca
      721 taatacctaa gtcaatacca attgtggaat tagttttagg taattcgata atttcttctt
      781 tacacaacaa agaaatatag tatttaccgc tagaatgacg tgatattgta gcagatttga
      841 taatcccttt tggttgtcta tgaagcttaa ttcttactaa tgacttcaat ttaggaactt
      901 tgatgaattt actatcaatc aaagcaactg taccattttg attattcgtt gtataactct
      961 gaacaggatt tttcttactt ttgaaacgtg gaaatccaac ggatttatcc cgaaaaaaat
     1021 tcttgtatgc tttatctaaa ttaagttggg cattggctaa agcaaggcta tcaatttctt
     1081 tcaaaaacgg aaattctttc ttgtatttag ctggtgtcgg aaaagtcatt tttttagaag
     1141 aatcattttt aacttcttca tacgcctttt ttcggtcatc aagcattaga ttgtagacct
     1201 tacggacaca accaaaagat ttggcaaaga agatttcttg ttcctctgtt ggatagattc
     1261 tgaatttgta tgcttttagt tgttccataa agttcaccta ctttctattt atgattgttt
     1321 ccttgatttt ggatatactt cttaatcaca tcaattggtg cgcc
//
LOCUS       DACDIE010000177         1338 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.182, whole genome shotgun sequence.
ACCESSION   DACDIE010000177 DACDIE010000000
VERSION     DACDIE010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1338
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.182"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1190
                     /locus_tag="IV250_002883"
     CDS             <1..1190
                     /locus_tag="IV250_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770105.1"
                     /translation="REKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQI
                     KNDIKFVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSII
                     QALNNDITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQ
                     KTPISLLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAK
                     ENYAKVIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESW
                     EKGKYKAADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVN
                     AGENKIEITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKN
                     ENK"
BASE COUNT          477 a          198 c          237 g          426 t
ORIGIN      
        1 atagagaaaa aaaagtcatc gctttatctg tcagtataag tctacttgtg tatactcatg
       61 tcttaactac actattgtgt agtgcattga ttgcttgtat gattatagtt cgtttgtttt
      121 taagcaagca aataaaaaac gatataaaat ttgtggtcgt gaatttagta aaggctgttt
      181 ttttgacttg tgcattaact agctattttt ggtatcctat gttgcagcag atgaattatc
      241 aagaaataca cagtcccttc aaaacagatt tgcagctaga agcactttct atttcagata
      301 gtatcattca agcgttaaat aacgatatta caacatacac tatggggtta ttaggattgg
      361 ttgcgttgat ttttccttta ttcatactga aagaaatgga gaaaaaagaa aaaatcatct
      421 atgttggcgt agtttctaca tgggtatgcg taactaattt atttccatgg tttttgtttc
      481 aaaaaacacc gataagctta ctccagtttc cttggcgtat cttaggtatc caaatctttt
      541 tttcttcaat gatcataacg cttgttttta tgaaaaagac tatgaataaa aaatattcat
      601 ggttaatcat tggcgctact gtggtgtttc tactcgtcac gtctatagca gcaaaagaaa
      661 attacgcaaa agttatccaa tcaaaacacg gtcatattgt tatcaatgaa gaaacactcc
      721 cacgttatac aacatcagat atgggaggat tatacgatta cgccccaaca gaagcgataa
      781 aagaaaggcc tcacttagaa aaacatgaag taaaagtagg agaatcgtgg gaaaaaggga
      841 agtataaagc tgcagataat aacattactt acacagtggc aagcaacaaa aagcaaaaag
      901 tgacagtacc agtatatgct tatttaggta cacaggtaaa agtaaacggt aaagtcgtaa
      961 atgactctta taaaaaacat ggcttagtga atgtggatgt aaatgctgga gaaaataaga
     1021 tagaaattac aacaaaatat actccaactg ctctcatggc tttatggatc agtattctga
     1081 cttattgttt agtggtgtat tcaagtttta aacgatttgc acctaataag ttgaaagatt
     1141 ctcgatatgt gactaaaaag gagataaaca agaagaatga aaataaataa aaaagtagta
     1201 atattgagtg gattaattat agttagtagt atatattctt tcatcttttt caaaaataca
     1261 cagagtattt ataccactgg agatctttct tttcacttat ctcgaatcaa ggggttatcg
     1321 actattttta ccagtccg
//
LOCUS       DACDIE010000178         1337 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.183, whole genome shotgun sequence.
ACCESSION   DACDIE010000178 DACDIE010000000
VERSION     DACDIE010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.183"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            87..1166
                     /locus_tag="IV250_002884"
     CDS             87..1166
                     /locus_tag="IV250_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8770106.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
BASE COUNT          361 a          357 c          369 g          250 t
ORIGIN      
        1 tagccatttg gaaataaaaa aacgccacgt gaattccatg ttatattgaa gttaccacaa
       61 ctctcaatag acaaggatga attcacatga cgcaaaacca tgataacaca gtttcacgca
      121 agggaaaaca cctaacctac tcagaacgtt gccaaatcgc catcctgaaa aaggaaaact
      181 attccaaccg tcaggttgcg gatgtcctgg gctgcgtgcc gcagaccata aacaacgaaa
      241 tcaaacgcgg gaccgttact cagcttaacc gccagaatca gaacgggaag gtctacgact
      301 attatcatga cgtctacgac gccgatgccg ggcaggcggc ctacgagaag aaccgcttga
      361 actgcggcag acggccctta tgggccgaca cggacgcctt cattgaatgg gcggacgaca
      421 tgatgctgtt ggaaaaatgg tccccggacg tggtggtggg ctttgccaga aaacacgaat
      481 tgttcgaccc ggccattatc cctgggacca ccaccctcta tcagtggatc gaccgtggca
      541 tcatgaaaac gaagaatatg gacctgctag aaaaactgtc ccgcaaaccg aaatcgtccc
      601 gtcatggcaa ccgcccgaat aaacgggtgc tagggaaatc cattgaagag cgcccggagg
      661 cggttggtac ccggaaaacc ttcggccatt gggaagtcga cacggtcgta ggaaacaaga
      721 ccaagtcgga tgccgtgctg ctgacgttgg tggaacggca gacacgcttc gaggtcattc
      781 tgaagctggc cgggaaggat gccgaatcgg tcaaccaagc ggtccgacag ctgagagaac
      841 gggctggtgc gtacttcggc cagctgttca agacggttac ctcggacaac ggctcggagt
      901 tcgcgggact gcacgatgcc ttgcaggatg tggcaaacat ctacttcagc cacccctatg
      961 cttcctggga gcgcgggacg agcgaaaacc aacacaagct gattcgccgt ttcatcccca
     1021 agggcagccc aatcggcaac gtgagcgaaa accaaatctt gcggattcag cgttggatga
     1081 acgattaccc gcgaaagatt ttgggctatg caacgccgca tgacacgttc gtccaggcat
     1141 ttaggcagga acggttgacg gcttgacacg tgggcccttg acaacgagcc tccctcggca
     1201 tggcttgtgt ctggaaagct ggccggggcc agccagtcgg gagactacca tgcctcagct
     1261 cattgtcaag ggcccactcg attagaaaaa ttagacggtt ggtggtaacg agcaaccggg
     1321 tcaccatggg tggctaa
//
LOCUS       DACDIE010000179         1294 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.184, whole genome shotgun sequence.
ACCESSION   DACDIE010000179 DACDIE010000000
VERSION     DACDIE010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.184"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1244
                     /locus_tag="IV250_002885"
     CDS             <1..1244
                     /locus_tag="IV250_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAP8770107.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQHFSRN"
BASE COUNT          423 a          233 c          277 g          361 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1021 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1081 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1141 ttctatttga taagatacca aatcttcccg tactttcagc agaaacttta gatcaactgt
     1201 tgccttggaa caaggatgta caacaacatt tttctcgtaa ctaaaaacgc caaacattcc
     1261 cgattctatt ctataggtcg ggttatttga cgta
//
LOCUS       DACDIE010000180         1288 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.185, whole genome shotgun sequence.
ACCESSION   DACDIE010000180 DACDIE010000000
VERSION     DACDIE010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.185"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(15..350)
                     /locus_tag="IV250_002886"
                     /pseudo
     CDS             complement(15..350)
                     /locus_tag="IV250_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313007.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(447..899)
                     /locus_tag="IV250_002887"
     CDS             complement(447..899)
                     /locus_tag="IV250_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770108.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKITSKNEVFNYSLTRINYKEITKFSLEKVPFWG
                     EFCLKIKCNWKRMYFYIDGDDALTFGKTTFSSFNFQFLLKNNFYGLLK"
     gene            complement(906..>1288)
                     /locus_tag="IV250_002888"
     CDS             complement(906..>1288)
                     /locus_tag="IV250_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP8770109.1"
                     /translation="GFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRME
                     KVDWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKA
                     AAKELAKVNIVAGIAAAAASCALE"
BASE COUNT          455 a          252 c          137 g          444 t
ORIGIN      
        1 attttttatc accagtaacc aattgagaac agccctaaag cagaatagtc ttttatctca
       61 aaacgcttaa tttcaggctt aggaatatga ataatctttt gagtatattc tttctctttt
      121 aatcttactg aagaaatacc actgcctatc ttttttacaa atatttcatt tggtgtacag
      181 actaataaaa caatatttgt aatcaatcct tgcactaaag aattaaaagg aagactattt
      241 aaaacagggt gtgcataact ataacatgtg aagtagttat tttgaacttt aacttttaaa
      301 aactttaaaa ataatcttat ttgttcttct aaacaatcat ctatactcat ataatcactc
      361 cactaattaa tctccccaaa aagacacagc attaagagat ataccaacac aacagtgcaa
      421 ataactctac atcttccttc gcaaccttat ttcaataaac cataaaaatt attttttaat
      481 agaaactgaa aattaaaact agaaaaagta gttttaccga atgtaagagc atcatcacca
      541 tcaatataga aatacattct tttccagtta catttaattt ttaaacaaaa ttctccccaa
      601 aaaggaactt tttctagtga aaacttggta atctctttgt aattaattct tgttaaagaa
      661 taattaaaca cttcattttt acttgttatt ttttcattga ctataactag ttcatctggt
      721 aaaaaaatta accaacaaac atcgacaaac agtaaatgat ataatccatt tccacctcca
      781 agcatattca atactggtga acatgcctta gaaacacgaa tatagttatg gtttaaatag
      841 atattttctt taaacattgc ttgtttaaaa gccatatgct cttgttctaa acttttcata
      901 aacttttact ctaatgcaca gctagcagca gctgctgcaa ttcctgctac aatatttacc
      961 ttagctaact ctttagctgc agcttttcct aattgttttt ctgcaatttt taataataaa
     1021 tccgcagctt tttcaaaatt aaaagctttt aatgcttcaa taaaatctcc ctgcgtacct
     1081 atagcccaag ccactcctcc tccaggaaga gaattaatgg ctacacatcc agcatatcgt
     1141 ttccaatcaa ctttctccat tctaggaaca ataatatgtt cataaaagac tcttgctgct
     1201 cttccttgtt cgttatcttg actgattcgt tcacgaatag caccaaaatc ctttatatgg
     1261 tatacaccat gttcgtctaa aaaaccaa
//
LOCUS       DACDIE010000181         1251 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.186, whole genome shotgun sequence.
ACCESSION   DACDIE010000181 DACDIE010000000
VERSION     DACDIE010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1251
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.186"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..456
                     /locus_tag="IV250_002889"
     CDS             <1..456
                     /locus_tag="IV250_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770110.1"
                     /translation="LFSCLNFRFTQKNKKNKLSIEQMKKDIETDLKNDHKERRKIEEY
                     TSLMEKCKATDAIFSFLRKSDEIITRENIDLENEISKLESIFSDINSLKYLIVEENSD
                     SFLEFEKYLTDLIDNRNNELMRQNIARFLTKLVSEWKSIISQNMDKLRY"
     gene            727..>1251
                     /locus_tag="IV250_002890"
     CDS             727..>1251
                     /locus_tag="IV250_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8770111.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIV"
BASE COUNT          484 a          162 c          211 g          394 t
ORIGIN      
        1 cttttttcgt gtttgaattt tagatttact caaaagaaca aaaagaacaa actcagtata
       61 gaacaaatga aaaaagacat tgaaactgat ttaaaaaatg accataaaga aagaagaaaa
      121 attgaagaat atacttcttt aatggaaaag tgtaaagcaa ctgatgcaat ttttagcttt
      181 ttaagaaaaa gtgatgagat aatcacacgt gaaaatatag atttagaaaa tgagattagt
      241 aaacttgaat ctatcttttc cgatataaat tctcttaagt atttaatcgt cgaagaaaat
      301 tcggatagct ttttagaatt tgaaaaatat ctaacagatc ttatagataa tagaaataat
      361 gaattaatga gacaaaacat tgcaagattc cttactaaat tagtttctga atggaaaagt
      421 attatttctc aaaatatgga taaattaaga tattaaggat gttagtttta aaataaacta
      481 atgtccttat ttttgtgttc acatttctgt tacctaagaa atacttaaag gaggtgatgt
      541 agccagcaac gactacaact tgaacccgct atctacagtt tatttgttgt ttcttttggg
      601 tatactctaa tggtacaaat ccatatttgc ttaaagcgtt gatttttcag cggtttttct
      661 ttattgcaaa gtgtacccta agacctgtat actaatagta aatagattgg ataggaatga
      721 tggcaaatgt atttagctta tttaagagtt tcttccgacg atcaaagttt agctcggcaa
      781 cataagctaa ttgaaaattg gtcagtcaaa aataatattg ctgaagacga tttaaaaatt
      841 ttcgaggaaa aagtctctgg aaaaaatatt gaaaacagac cagagctgca agagcttatt
      901 tctttcatac gtgaacgaga tacgattatt attcctagtc ttgatcgatt agcaagaaat
      961 agtaaagata caaaagactt acttcaattg tttcgtagta aaggtgcttc tattgaaata
     1021 ttagacctac cgtcgtttaa tggggtaact gaccctgcat taagagactt gctaacaaat
     1081 ttagtcatag aagtgtttag ctatgttgct gaaaacgaac ggaaaaaaat caaagagcgt
     1141 caaaaacaag gcattgaaat tgcaaagaaa aatggtcgtt atactggtaa aaagattcaa
     1201 taccatgcca atgcaacagg aaaaaacaaa ttgatttacg atgaaattgt t
//
LOCUS       DACDIE010000182         1246 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.187, whole genome shotgun sequence.
ACCESSION   DACDIE010000182 DACDIE010000000
VERSION     DACDIE010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.187"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            200..742
                     /locus_tag="IV250_002891"
     CDS             200..742
                     /locus_tag="IV250_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770112.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            832..>1246
                     /locus_tag="IV250_002892"
     CDS             832..>1246
                     /locus_tag="IV250_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HAP8770113.1"
                     /translation="MDELKRTEIQIIDKKERPKNGFVLSEKTDLENTEAFNKESVKDL
                     RKLPTKDINGKDFTSKDFEKYDLTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIV
                     GVVTDPVDDNGENKEAIEKSKLIHEKTKASYPFLMP"
BASE COUNT          514 a          141 c          238 g          353 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccag
//
LOCUS       DACDIE010000183         1231 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.188, whole genome shotgun sequence.
ACCESSION   DACDIE010000183 DACDIE010000000
VERSION     DACDIE010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1231
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.188"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>1231
                     /locus_tag="IV250_002893"
     rRNA            <1..>1231
                     /locus_tag="IV250_002893"
                     /product="23S ribosomal RNA"
BASE COUNT          323 a          280 c          375 g          253 t
ORIGIN      
        1 gaaataatag taccgaagtt cctgatgtca cactgccgag aaaagcttct agtgagaaaa
       61 cagcggcccg taccgcaaac cgacacaggt agtcgaggag agaatcctaa ggtgagcgag
      121 agaactctcg ttaaggaact cggcaaaatg accccgtaac ttcgggagaa ggggtgctga
      181 tcatacgatc agccgcagtg aataggccca agcgactgtt tatcaaaaac acaggtctct
      241 gcaaaatcgt aagatgaagt ataggggctg acgcctgccc ggtgctggaa ggttaagagg
      301 agtgcttagc gcaagcgaag gtacgaattg aagccccagt aaacggcggc cgtaactata
      361 acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa
      421 cgatttgggc actgtctcaa cgagagactc ggtgaaattt tagtacctgt gaagatgcag
      481 gttacccgcg acaggacgga aagaccccat ggagctttac tgtagtttga tattgagtgt
      541 ctgtaccgca tgtacaggat aggtaggagc cgtagaaatc ggaacgctag tttcgatgga
      601 ggcgctggtg ggatactacc cctgcgttat ggccactcta acccgcacca ctaatcgtgg
      661 tgggagacag tgtcagatgg gcagtttgac tggggcggtc gcctcctaaa aggtaacgga
      721 ggcgcccaaa ggttccctca gaatggttgg aaatcattcg aagagtgtaa aggcagaagg
      781 gagcttgact gcgagaccaa caagtcgagc agggacgaaa gtcgggctta gtgatccggt
      841 ggttccgcat ggaagggcca tcgctcaacg gataaaagct accctgggga taacaggctt
      901 atctccccca agagtccaca tcgacgggga ggtttggcac ctcgatgtcg gctcgtcgca
      961 tcctggggct gtagtcggtc ccaagggttg ggctgttcgc ccattaaagc ggcacgcgag
     1021 ctgggttcag aacgtcgtga gacagttcgg tccctatccg tcgcgggcgt tggaaatttg
     1081 agaggagctg tccttagtac gagaggaccg ggatggactt accgctggtg taccagttgt
     1141 tctgccaagg gcattgctgg gtagctatgt agggaaggga taaacgctga aagcatctaa
     1201 gtgtgaagcc cacctcaaga tgagatttcc c
//
LOCUS       DACDIE010000184         1215 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.189, whole genome shotgun sequence.
ACCESSION   DACDIE010000184 DACDIE010000000
VERSION     DACDIE010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.189"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            71..1027
                     /locus_tag="IV250_002894"
     CDS             71..1027
                     /locus_tag="IV250_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770114.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
     gene            complement(1081..>1215)
                     /locus_tag="IV250_002895"
     CDS             complement(1081..>1215)
                     /locus_tag="IV250_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8770115.1"
                     /translation="FNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA
                     "
BASE COUNT          427 a          179 c          208 g          401 t
ORIGIN      
        1 gttgacaggg aatcctaaaa acattcggct ttttttatat aatcttttta actcaacaga
       61 gataagtttt gtggaattgt atacgctacc tataattata aaaacagaaa aaatgttaaa
      121 agatttaaaa cagtattatc ctctgtctgc ttatgaaaaa aagaaattat tccacttttt
      181 agcaatcaac ctgttggctg aacaagctga ttctttagat attgctagca atcagtcgct
      241 atttttttcg gatataggat tttaccactt aaaaggagta ttaggagaaa aatccgttat
      301 ttttatcaat aagacactct cttggcttta tcttcatgat aaattggatc aaaaatactt
      361 gagaaaaaat gaagatgaaa caatcgaatg gttgaatgaa cgattcattg aaagttttga
      421 aaatcagttt aatctgttgc gagaagagac gagccaattg atccgaagag agttaaccaa
      481 aatacatttt aaacttttgc attacactat taataaagct gatgaatttc cacttgatgt
      541 ttcttttttt agagaaaact atccagcatt ttattgctat ttgatagagt atataaatga
      601 tctaacgtca aaacgcaaag agttgcttca agataaattt tttctttttt tcagttattt
      661 attattattg atcaatcaca tacccattca actgatttct gaaccagtaa agatcatcat
      721 cgatttttct tatggtgctg cctataatca atttatcaag aaaaatttaa gcttgtacat
      781 taatctgaac acggaagtga tagaggcagg aaagcctgag ttcccggatg ttataatcac
      841 gaataggaat aatctatatg aagaaagtgc atctcaagta gtggtttggc tcgatccgcc
      901 aagagcagta gactggggga atcttacaaa gattttgttg actatccaag aagagaaata
      961 caaagacagc aaaattgaca agcaaataga cgattttcct gaagaaatca tcgacaggat
     1021 cgaatagtga acttctttta gaaagtccga ctgctttttc taaaaatatt atttgtcaaa
     1081 ttaagcgaga caaaactttc catttacgta actcaaaaaa acttctaatg gtgtttggta
     1141 atttagtgat tttctaggga tatggtttct tttggacgca acggatgaga tgaatccttg
     1201 attgacttgg ttgaa
//
LOCUS       DACDIE010000185         1205 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.190, whole genome shotgun sequence.
ACCESSION   DACDIE010000185 DACDIE010000000
VERSION     DACDIE010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1205
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.190"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..170
                     /locus_tag="IV250_002896"
     CDS             <1..170
                     /locus_tag="IV250_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HAP8770116.1"
                     /translation="SNYIFDGQCGIIKMRDYLILDIDSEEDFKWLEFLFPKFLEDSSE
                     LQDIYNYLYRR"
     gene            175..1122
                     /locus_tag="IV250_002897"
     CDS             175..1122
                     /locus_tag="IV250_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAP8770117.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          391 a          172 c          256 g          386 t
ORIGIN      
        1 aaagtaatta tatttttgat gggcaatgtg ggataataaa aatgagagat tatctaattt
       61 tagatattga tagtgaagaa gattttaaat ggttagaatt tttgtttcct aaatttttag
      121 aagattcttc ggagcttcaa gatatctata attatttata tcggaggtaa aaggatgagc
      181 tgttatctta tcaccggtgg ggctggcttc attggttcta ccttagccaa tcatttagga
      241 gaagaacatg tggtaatcgt cgtcgatgac ttatcaatgg gaaaagtaga aaatcttgat
      301 atgacgaaaa atatcacttt tattgaggga gatgtcgctg agaaatcctt gatggaagag
      361 atcatgaaag ctaataaatt tgattatatc tttcatttag cagcagttgc tagtgtggca
      421 gattcagttg cccgaccagt agaaacacat cgtgtgaatt ttgaaagtgt attaatgatt
      481 ttagaattga tccgcaagta tcaacctgat ttaaaacgga tcgtgtttag ttcttcagcg
      541 gctgtttatg gggatgaacc gactttacca aaaaaagaag agtccgtgat tcgcccattg
      601 acgccttatg cggttgataa attcgctgca gaacaatatg tgctagatta ttgtcattta
      661 tatgatgtac ccggtagcgc agttcgtttt ttcaatgtct atggtccaaa tcaaaatccg
      721 aattctccat acagtggtgt gatttctatt ctcgtagatc gctataagaa acaaatcgta
      781 ggagaagcaa cttcatttac attatttggt gatggaagcc agtctagaga ctttgtttat
      841 atagatgatg taatccaagc attactttta gttgcgaatg aggaagctgc actggggaaa
      901 caattcaatg ttggaacggg taaagcaacc acacttttgg aattgattca tgcaatcaat
      961 gaaattctcg gtgtagaatt gacacttgaa tacaaagaag agcgttcggg tgatatccat
     1021 gactctctag cagatattac taagatccag tcaattggct atcaaccgaa atatggtatt
     1081 ttaagtggaa tgactaatta tttgaaaact gaaatcaatt aaaacatact aagataagat
     1141 tagagggtaa attcaggctc tttgtcaata aggactgatg agttttgtaa aatcaaatct
     1201 gggat
//
LOCUS       DACDIE010000186         1182 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.191, whole genome shotgun sequence.
ACCESSION   DACDIE010000186 DACDIE010000000
VERSION     DACDIE010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1182
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.191"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..643
                     /locus_tag="IV250_002898"
     CDS             <1..643
                     /locus_tag="IV250_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP8770118.1"
                     /translation="GIILSVIWMSTNNAWVPWFYGEMSKNNEKKVKKVSNYYLVVFTL
                     ITMIVMVVSIDLAKFMAPKDYYSGLSLVVPITLGYFFQFLYSLPVNAEFYEKKTSFIA
                     IGTVASAIVNILLNMIFIPRLGIIAAGYTTVFTYFLLFIFHYNIAKKITNKQLFDTKK
                     ICIIIIFVSIFSLILHILINQMLIRYVLTSLLIISLVIYFLKIKNRIIDDYNS"
     gene            656..>1182
                     /locus_tag="IV250_002899"
     CDS             656..>1182
                     /locus_tag="IV250_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HAP8770119.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSV"
BASE COUNT          420 a          140 c          211 g          411 t
ORIGIN      
        1 aggtattatt ctaagcgtaa tatggatgtc tactaataac gcctgggttc catggttcta
       61 tggtgagatg agtaaaaata atgaaaaaaa agtgaagaaa gttagtaact attatttagt
      121 agtctttact ttgattacga tgattgtaat ggtagtttcg attgatttag caaaatttat
      181 ggctccgaaa gactattatt ctgggttatc tttagtggtt ccgataacct taggctattt
      241 ctttcagttt ttatatagtt taccagttaa tgctgaattt tatgagaaaa aaacatcgtt
      301 tattgcgatt ggtacagtag caagtgctat agttaatata ctattaaaca tgattttcat
      361 acctaggtta ggaataattg ctgctggata cacaaccgta tttacgtatt ttttactttt
      421 tatttttcat tataatatcg ctaaaaaaat tacgaataaa caattatttg acacaaaaaa
      481 aatatgtatc ataataattt ttgtatcaat tttttctttg atacttcata ttttaatcaa
      541 tcaaatgttg attcgttacg ttttgacatc attattgata attagtttag ttatatattt
      601 tttaaagatt aagaatcgaa ttatagatga ctataattca taggaggaga tataagtgtt
      661 taaaggaaaa acaatattag taactggcgg aactggttcc attggaagtg aaattgttcg
      721 tcagctttta aaatatgaac caaaagctat cagaatttat agtagaggtg aagaaaaaca
      781 attttatatg caacaagagc tctcaaatca cactaatttg agatttttaa ttggtgatat
      841 cagagactct caaaggattg attatgcatg tcgtgatgtg gatattattt ttcatgctgc
      901 cgcaatgaaa cacgttccag caagtgaata taatcctatg gaagctgtaa aaacaaatgt
      961 gatgggaact caaaatgtaa tagatgcagc gatcaaaaat aatgtaaaac attttgtagg
     1021 tatcagtaca gataaagtag tgagtcctac taacaccatg ggtgcgacaa agttattgag
     1081 tgaaaaatta ataatagctg ccaatcagta taaaggaaaa acagaaacaa tattttcttg
     1141 tgtaaggttt ggaaatgtca tggggtcaag tggatctgtt at
//
LOCUS       DACDIE010000187         1173 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.192, whole genome shotgun sequence.
ACCESSION   DACDIE010000187 DACDIE010000000
VERSION     DACDIE010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.192"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..827
                     /locus_tag="IV250_002900"
     CDS             <1..827
                     /locus_tag="IV250_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8770120.1"
                     /translation="LTDLSPALLQNFINAEFKKGYSKKTMTITHSVLKNALNMAVYPW
                     GLIKQNPMLYVKIPKYEARPTTKKDLKIISLEDFDHMLEITPEGHPFYIPLNIGFYTG
                     MRVGEVCGLTWDDVDFSNGTITVEKQMVKNDGAWVYGTPKTSSSNRTIFIGQTLLAIL
                     KKHKKQQLENRMKYGKLYIDSNAVCTKEDGELVTPSVVKWNTRRISNALSLSFNFHSL
                     RHTHATLLLENGAKMKEISERLGHSRISITMDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1045..>1173)
                     /locus_tag="IV250_002901"
     CDS             complement(1045..>1173)
                     /locus_tag="IV250_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8770121.1"
                     /translation="KNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
BASE COUNT          401 a          214 c          221 g          337 t
ORIGIN      
        1 aacttacgga tctctcccct gctcttttac aaaatttcat caatgctgaa tttaaaaagg
       61 gttactcgaa gaaaacaatg actattactc actctgtcct taagaatgcg ctgaatatgg
      121 cggtttatcc ttggggatta atcaagcaaa atcctatgct gtatgtaaaa ataccaaaat
      181 atgaagcacg accaacgact aaaaaagatc taaaaatcat ttctcttgag gactttgatc
      241 atatgctaga aattactcct gaaggacatc ctttctatat tcctttgaat attggatttt
      301 atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg ggatgatgtc gatttttcaa
      361 atggaacaat tactgtagag aaacaaatgg taaagaatga cggcgcatgg gtatatggta
      421 cgccaaagac aagcagttcc aatcgaacga tttttattgg acaaaccttg ctagcaattc
      481 tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa gtatggaaag ctctacattg
      541 attcaaatgc agtatgtacg aaggaagacg gtgagctagt tacgccaagt gtagtgaaat
      601 ggaatactag gaggatatcg aatgcactct ccctctcttt taacttccat tctctcagac
      661 atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat gaaagaaatc tctgaacgat
      721 tgggccacag cagaatttca attacgatgg atacttactc gcatgtaaca gataagatga
      781 gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa ttcttgattt ttgccaccga
      841 aaaaaaccac cggtggcaaa ccggtggcaa atccatgtaa acatggttta ttttttacac
      901 tgttttcaaa caaacccttg ctatttcttc ggttcgtcct cgtccatttt cgataccgac
      961 agctagctta taacccaagg tatatcaaca taaaaaaata aaaataggct atgacagcag
     1021 cgtgacagca aaaaagatgg aaagttaaat atcaaacctc gtaatttcat ttgcgagtgc
     1081 ttgatttgtt tctggatata agtgtccata tttgtccaaa gtagttttaa tagaggcatg
     1141 acctagacgc ttagatattg catagatatt ttt
//
LOCUS       DACDIE010000188         1172 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.193, whole genome shotgun sequence.
ACCESSION   DACDIE010000188 DACDIE010000000
VERSION     DACDIE010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1172
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.193"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..1050)
                     /locus_tag="IV250_002902"
     CDS             complement(<1..1050)
                     /locus_tag="IV250_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8770122.1"
                     /translation="MIERHVNLLKLLSRQEDFKPASFFSDKLAVSTKTIYQDIELLED
                     YVVNYQVTIERLPSLGIKLIGDRKNVEHLISSVENYLEKDIYSPEKRRVEIIKEVLFG
                     DKPVSLTHLSQSFMVSKTSLYNDLRIINKIIGSGEVKLQSSAEGISFTGNEQAIQRAI
                     KQLVFYYANDMESKTFFQVLNMLFDSKVISVIHDLLFNDYSELTEKVSDYYVRSLIST
                     LVIQCNRLLKGHHLTVEEEFLFNSIRYMETYIVANGLIEELKAKLNLPFYDADKEYLC
                     RQLFAHRVTNNLKTSNEEYAMSVHKLIERMSEIEKIDLTQNDRLYQSLLYHVPAMVLR
                     LKKGIKIQNPLLENIK"
BASE COUNT          386 a          212 c          156 g          418 t
ORIGIN      
        1 tttgatattt tctaataacg gattttgaat tttaatacct ttttttaaac gaaggaccat
       61 tgctggcaca tgatacaata aggattgata taatcgatca ttttgagtca aatcaatttt
      121 ttcaatctca ctcattcttt caatcaattt atgaacgctc atagcgtact cttcattgct
      181 ggtttttaaa ttatttgtca cacgatgtgc aaatagttga cgacatagat attctttatc
      241 tgcgtcataa aatggaagat tcaattttgc cttcaattct tcaattaaac catttgccac
      301 aatgtacgtt tccatatagc gaatgctatt gaagaggaat tcttcttcaa cagttaaatg
      361 atgacctttc agtaaacggt tacattggat aactaaagta gaaatcaatg accgaacata
      421 gtaatcagaa accttttcag tcagttcact gtaatcattg aatagcaaat catgaatgac
      481 actaataact tttgaatcaa acaacatatt taaaacttga aaaaatgttt ttgattccat
      541 gtcattcgca taatagaata caagttgctt gattgctcgt tgaattgctt gttcattacc
      601 agtaaaacta attccttctg cagatgactg caacttaact tctccactgc caataatttt
      661 attgataatt ctcaggtcat tgtataacga tgttttacta accataaaac tttgagacaa
      721 atgagttaaa gaaacaggct tatcaccaaa caaaacttct ttaatgatct cgacacgtct
      781 tttttctgga gaataaatat ctttttcaag ataattttct accgatgaaa tcaaatgttc
      841 aacgtttttc ctatcaccta tgagtttgat acccaaagaa ggtaatcgct caattgttac
      901 ttggtaatta actacataat cttccaacaa ttcaatatct tgataaatgg tcttggtcga
      961 cactgccaat ttatcactaa aaaaagaagc gggtttaaag tcttcttgtc gacttaataa
     1021 ttttaataaa tttacatgtc tttcaatcaa acttgttcac ctcttaacgc tagcatttgt
     1081 tacctctttt cattatttat tctagcactg gaaccccctt ccctcaatta catctatgtc
     1141 aataatactt gtggaagttt gttgtaaagg aa
//
LOCUS       DACDIE010000189         1140 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.194, whole genome shotgun sequence.
ACCESSION   DACDIE010000189 DACDIE010000000
VERSION     DACDIE010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.194"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
BASE COUNT          410 a          142 c          201 g          387 t
ORIGIN      
        1 tgtccattat tcattaaaat gttcttcata tattcttctt tggcttattt tttcattgtt
       61 tgaattgcta aatttaatag caatatcttc atatcactta gataagctaa ataccaaatc
      121 tagccgttat aacgaagtgt ttaaggaaaa tgtgaagtta gaaagtgact taaacaagct
      181 aaaaaaataa aaagaagaaa acataagtta actaaaatta ttttattaag aatgatggaa
      241 tataaggata aggttaccaa cataacgatc attacttctc tgatatgcta tggacttcta
      301 tcaatcagta ttttgtctat agattatgga gtaactatac tgcaagcctt agttttggct
      361 atgcctatgt atctatcttt gtttatcatg catgaaatat ataagctatt tgttttatca
      421 aaaaatgtag aaatacgggt aaatattttg gtgaatagtt atcaaaaaga actcaaagaa
      481 agaagacggt ggcggtattt attgaaagga tgaaaaagta ggatttgaca tactggtatt
      541 cagaaagtgc cgtcactttt aaaacaatgg aaaaggtgga gataagatga agaaaaaaca
      601 aactaaacta tcattagcag atatttaaca cagttaaccg caactgaaga tggtgttgtt
      661 gaatttgaac aatctgaaat tgcggtagta gatgatcgtt accttaaaat gccttacttc
      721 tttgaccaag caaaggtcat ttgcctttgt cactacgatg gggtacgtga ttttaggcgt
      781 taaaaagtag tgattatatc aactttacga gtaaatataa atttagacat gaaactgaat
      841 tatccaatgg aaaatcatgg tttataatct agaaaaatta tagtaatgat tggattatat
      901 atattctttc gtattttcgt acaactgtag cttgtcaaaa ttgagaaatt taaccgatta
      961 ggtactttac atcgttattt gggtaatttc taaaacatga agcttaccaa cgaataaact
     1021 gagactatct tattaattga tcaatataag aaaatatctt tgaggagatg atgtactatg
     1081 ttttcgtcta tccacgattg gttaggtggt cttttcggcg gtggaggtaa aggtttttaa
//
LOCUS       DACDIE010000190         1127 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.195, whole genome shotgun sequence.
ACCESSION   DACDIE010000190 DACDIE010000000
VERSION     DACDIE010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.195"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>1127
                     /locus_tag="IV250_002903"
     CDS             <1..>1127
                     /locus_tag="IV250_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770123.1"
                     /translation="YIKDQRNYSAHPVVTYNGNTWELRDITKETCQDVIRKSYEIIFL
                     KNPILGKKVTIEIIQYASRIHAMSVTPEEFHSALKNSYLNKLSEKAKENLCKTAFKFV
                     FQLSYGNEEADRNRESTFRLLNALIFDNKEFYLNILKKEIAKYRFTAESREEINKSLG
                     ENQQITYTKGFFLLTFIATHPELQHDFSDETIQNLQISINDFKPKQQVLTEIEYYYRL
                     RSLAVLGNNNEELQNHLNSLVDPILQQKMRVSSLPSSTLETIYNQYLYFGEKEMFIDF
                     MIEHLTDASSFRLADKDMEVLPWIYNKLNQEQFNKLLYNLNGNNQFYKNSNFPPFISN
                     LLDFYNQKFQVNLRKHYSGLLYPNAVTMDQGFQLKDKELKKL"
BASE COUNT          456 a          171 c          160 g          340 t
ORIGIN      
        1 tatattaagg atcaaagaaa ttattcagct catcctgtag tcacttacaa cggtaatact
       61 tgggaattaa gagatataac aaaagaaact tgtcaggatg ttatacggaa gtcctatgaa
      121 attatattcc taaaaaatcc aatattagga aaaaaagtaa cgatcgaaat tatacaatat
      181 gcaagtcgca tacatgctat gtctgttaca cctgaagaat ttcactctgc actaaaaaat
      241 agctatctaa ataaactaag cgaaaaagcc aaagaaaacc tatgtaagac agcctttaag
      301 tttgtttttc aattatctta tggaaatgag gaagcagata gaaataggga aagtactttc
      361 agactactta atgccctaat ttttgataat aaagaattct atttaaatat tctaaaaaaa
      421 gaaattgcaa agtataggtt cacagctgaa tcaagagagg aaattaataa atcacttggt
      481 gaaaatcaac agatcacgta tacaaaaggg ttctttttac ttacttttat agctacccat
      541 ccagaattac aacatgattt ttcagacgag acaattcaaa atttacaaat atcaataaat
      601 gattttaaac cgaaacaaca agtactaaca gaaatagaat attactaccg tttacgttcc
      661 cttgcagttc taggaaacaa caatgaagaa ttacaaaatc acttaaatag tctagttgat
      721 cctatactcc agcaaaaaat gagagtttct tcgctaccat cttcaacatt agaaacaata
      781 tataatcaat atttatattt tggtgaaaaa gagatgttca tagattttat gatcgaacat
      841 cttaccgatg ctagttcatt caggttagca gataaggata tggaagtatt accatggatt
      901 tacaataaat taaatcagga gcaatttaac aaattactat acaacctaaa tggaaataat
      961 caattttaca aaaacagtaa ttttccgcct tttatttcta atctgttaga cttttataat
     1021 cagaaattcc aagtgaattt acgtaaacat tatagtgggc ttctatatcc aaatgcagtg
     1081 actatggatc agggttttca attaaaggat aaagaattaa aaaaact
//
LOCUS       DACDIE010000191         1122 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.196, whole genome shotgun sequence.
ACCESSION   DACDIE010000191 DACDIE010000000
VERSION     DACDIE010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1122
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.196"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(339..794)
                     /gene="tnpA"
                     /locus_tag="IV250_002904"
     CDS             complement(339..794)
                     /gene="tnpA"
                     /locus_tag="IV250_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAP8770124.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
BASE COUNT          317 a          220 c          190 g          395 t
ORIGIN      
        1 atacttacgt aactcaaaaa tacttccaaa ggtgttcgat aatccagtga ttttctagga
       61 agattatttc tgcgccagca ttttctaatg tttgaatttg tgttttgtaa tcttggttgc
      121 ttgtgctaat tcttgaatag ccgtaaattg ccaaaatttc atcacccttt ctggacataa
      181 gtttctgaca ccagttaaaa tcaagtctat cagtaaccca tacacccgtg aggacgggtg
      241 tatttttaat ccccctagaa ggggtcgaca ctcgctgccc cttaaaaggg gatttttaaa
      301 agaccgccaa ctgctcatct tcactcactt acttctcttt attttttaaa tggttctacg
      361 tactctcttt tacttataat atatcgaatg cgatcatcag cttcttgttc acggatgtat
      421 ttttcatttg tcttttgatt caatccgact gtactcacat agtatctctt cgaccaaaag
      481 gatttgtttc cgtatttata tttgagattt gcgtgctgct cgtggattag taccgcacta
      541 cgacctttca gataccccat aaaactggat atacatattt ttggtggtac tcgaacaagc
      601 atgtgaatat gatctagcat tgcatgtgct tctatgattt ccacatcttt catttcgcac
      661 aacttcctaa gtatttttcc aagatctttt cgtaattgcc catagattac ttttcgttga
      721 tattttggtg taaaaataat atgatactta caattccaac gagtatatgc taaactttta
      781 tcgtcatttg acatgaaaaa actcctttcg atctgatgag cggttggtag ccactcacta
      841 gtatatcgaa aggagttttt atcgctaaag atacttcttc cacgcgtaga aacgcgcggt
      901 tttcttgttt cgcacgcttt acgcacaaaa ctgactgaag tcattaagaa aataaaaggg
      961 tctaaaactt ttaagttttg taccgacccc caaaagttag accaaaaaat ctaacttttg
     1021 gaggtcggta ttttttatgg ctaagtatac ttttgaactt aaattaaaga tcgttcatga
     1081 ttacttggat ggtaaaggcg gaagtgatta tctggcaaag aa
//
LOCUS       DACDIE010000192         1101 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.197, whole genome shotgun sequence.
ACCESSION   DACDIE010000192 DACDIE010000000
VERSION     DACDIE010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.197"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            79..234
                     /locus_tag="IV250_002905"
                     /pseudo
     CDS             79..234
                     /locus_tag="IV250_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
     gene            253..>1101
                     /locus_tag="IV250_002906"
     CDS             253..>1101
                     /locus_tag="IV250_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8770125.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGI"
BASE COUNT          462 a           99 c          191 g          349 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcagga taaggataag tgtattaatt gtggaaaatg taaaaaagtt
      121 tgccaaatga ataaagattc agttgagaat tgcaatcatc tggagtgtat cagatgtgga
      181 cgatgtaaaa atgcttgtcc agtggatgca attagttatg gagtaagaaa atagagagga
      241 gtagttagga aagtgataaa aaatgctata gcatatatta caagaaagaa aaatagaact
      301 ttaataattt ttatcatatt aactatagtt ctttcttgct tgtattcttg tttaacaata
      361 atgaaatcaa gcaatgaaat agaaaaggct ttatatgaaa gttctaattc ttcaatatca
      421 ataacaaaaa aagatggtaa atattttaat gttaatcaat ttaaagatat tgaaaaatta
      481 aaagaaattg aagaaaaaat aattcaatat gatggattag caaaactaaa agatgctaaa
      541 gtagttagcg gagaacaaag aataaataga gaagatttat ctgacgaatt taagaatgtt
      601 gtttcactcg aagctacaaa taatactaaa agaaatattt tatttagtag tggagtattt
      661 acaattaaag aaggtaaaaa tatagaagaa aatgataaga attcaattat tgttcatgaa
      721 gaatttgcta aacaaaacaa tctaaaatta ggtgatgaag ttgatcttga attactagat
      781 attgaaaaaa gtggaaaaat aaaaagtcat aaatttaaaa ttatagggat cttttctggt
      841 aaaaaacagg aaacatatac aggattatct tctgatttta gtgaaaatat ggtgtttgta
      901 gattattcaa caagccaaga gatattaaat aattcagaaa ataatgaaat tgcaaataaa
      961 attttaatgt attctggtag tgcagaatct acagacttag ccttaaacaa attgaaagag
     1021 cttaaaattg atgagtcaaa gtattttgtt gaaaaagatt ctaatgcgtt tgaagagtct
     1081 ttagagtcag tgagtggaat a
//
LOCUS       DACDIE010000193         1098 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.198, whole genome shotgun sequence.
ACCESSION   DACDIE010000193 DACDIE010000000
VERSION     DACDIE010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1098
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.198"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(148..221)
                     /locus_tag="IV250_002907"
     tRNA            complement(148..221)
                     /locus_tag="IV250_002907"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(185..187),aa:Ile,seq:cat)
     gene            complement(776..1090)
                     /locus_tag="IV250_002908"
     CDS             complement(776..1090)
                     /locus_tag="IV250_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770126.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT          411 a          173 c          169 g          345 t
ORIGIN      
        1 agtataacta tgtgaaacat cctttcatta atccataaaa ggatacataa aaaagccact
       61 catttgagtg gcaatgaaga aaagctttag cttgtataat actcttcaaa aaattctaac
      121 acagaacgat tcaattggct acattaatgt accctgtagg actcgaacct acgaccggac
      181 ggttatgagc cgtctgctct gaccaactga gctaagggta gtggttgttg ccacataaag
      241 ccataaacaa tcaaccagta gaatgcgtgg caacaaacct gttatcgcat atcttggagt
      301 gtgactattt atgagtgata gtgaagatat gcgtctccat atatgtatgg ttgagacaga
      361 taaacatgta tgaaaaacgt tgttaaatca acgttttttt attttggtca aggagagaaa
      421 agtagagaaa tacaaaattt tttgcattga ttttgcattc aaaaaaacat tcaaacattt
      481 agaaatcccc aatacgtatt caggaaaatc tattttaagg tttacaaaaa gaacttttgt
      541 tcgtacactc tttttgaggt gaacttttat gctgatggaa ggaaaaacac aattatggtt
      601 taagcatgtt ccttcaaata agttttttaa ttataaactc tagagaaatt aattcatcct
      661 aacataaaat aattgttcac tcaaaagttt ttctagaaca attaaaacag caggcttcac
      721 attcgctata catttgtaag aagtaataat tcctgtacta aaaggcataa ttgctttaat
      781 tattttttga cttctcttca aacacaagaa gtagttgtgt aataattgca gaaataagga
      841 ttccaataat agatataaaa tttgacatgg aaacgtctat aacattaaaa gaataaagtg
      901 ttgaagaaag taaactgatt gaaataaccc ctccgtaaaa aatagtctgt gaatataccc
      961 actttttttc agaagacgta cttcgtttgg ttctaaaacc tatccctgca tttctactct
     1021 tatcaaaaaa ccaaatattt agaagtaata atattgctat tattaagata aaattactgt
     1081 aaaaatccaa aagctatc
//
LOCUS       DACDIE010000194         1074 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.201, whole genome shotgun sequence.
ACCESSION   DACDIE010000194 DACDIE010000000
VERSION     DACDIE010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.201"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..430
                     /locus_tag="IV250_002909"
     CDS             <1..430
                     /locus_tag="IV250_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770127.1"
                     /translation="DENGQFFVWMRHEHCWCNPFVLKKENEIRTKIANGEWDYLFRWE
                     DESFIANDMDHAFVSFLDNTWGTWYDCYLEKLGFQNIDIDAIECLKEVDQKYEEEPYY
                     LLQQKLANNSVFQVEIWENEDGTYEGLAQEIPFSDRKSSI"
     gene            605..940
                     /locus_tag="IV250_002910"
     CDS             605..940
                     /locus_tag="IV250_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770128.1"
                     /translation="MKQYLNEWKAIEGSLVTQRIKELPDCLEKDHLFQIREMLKKEQF
                     DPDQFLVVEYPTIGVYCCNHINDGKYFIIQEYGGQLTPFYTTWEMSEEGINNFPCESI
                     EDSISHTEC"
BASE COUNT          391 a          129 c          219 g          335 t
ORIGIN      
        1 agatgagaat ggacaatttt ttgtttggat gcgacatgag cattgttggt gtaatccttt
       61 tgtgttaaaa aaagaaaatg aaatcagaac taaaattgca aatggtgagt gggattacct
      121 cttcagatgg gaagatgaat cttttatagc aaatgatatg gatcatgcat ttgtctcatt
      181 tctggataat acatggggta cttggtacga ttgttaccta gaaaaattgg gctttcaaaa
      241 tattgatatt gatgctattg aatgtcttaa agaagtcgat caaaagtatg aagaagaacc
      301 ctattatctc ttgcaacaaa aattagccaa taattcagtg tttcaggtag aaatttggga
      361 aaatgaagat ggaacttatg aggggctagc gcaagaaata cccttctcag ataggaagag
      421 ttcgatttga aaagagtata tcagcaattt ttatgaagtg aaagcaagct tacaatatag
      481 taatggaaaa ttgtggttag ctggaaggta tattccagct aaccaaatct tacgttttct
      541 gggagagtgg cgttttaaaa caatttccta ggaaaaatag ttaacataaa aggaatgaat
      601 tgttatgaaa caatatttaa atgaatggaa agcgatagag gggtcattag taacgcaaag
      661 aataaaggaa ttacctgatt gcttagagaa ggatcatttg tttcaaatac gagaaatgtt
      721 gaaaaaagaa cagtttgatc cagaccaatt tttagtagtc gagtatccaa ctattggagt
      781 gtactgttgt aaccatatca atgatggaaa atattttatt attcaggagt atggaggaca
      841 gttaactcct ttctatacta catgggaaat gagtgaagaa ggaatcaata attttccatg
      901 tgaaagcatc gaagattcaa tttcacacac cgaatgttaa ttgattataa ctataaaaaa
      961 ggattgaaca ttatgttcaa tccttttttc aatgaaatgt tttccgccat ttcattgttt
     1021 ggtgcgattt ctattttatt atatcaaaat aaagagagat tctaaagaaa gaag
//
LOCUS       DACDIE010000195         1048 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.202, whole genome shotgun sequence.
ACCESSION   DACDIE010000195 DACDIE010000000
VERSION     DACDIE010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1048
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.202"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            222..455
                     /locus_tag="IV250_002911"
     CDS             222..455
                     /locus_tag="IV250_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HAP8770129.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            452..859
                     /locus_tag="IV250_002912"
     CDS             452..859
                     /locus_tag="IV250_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAP8770130.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
BASE COUNT          402 a          137 c          178 g          331 t
ORIGIN      
        1 aaaaagcata taaacaggta tacgtttaat aatttaaaat aaatggtata cataaatagg
       61 gaaggaaata gaagggagca atctaaattt tttgaatttt cttaattaat tatatagaaa
      121 gaacaatctt tctatagagt tagacatgag agggactatg cttgatttaa aatcaacaag
      181 cgtatatact gtatatacaa agaaaaagga gggagaagac tatggcaatt aaagaaagaa
      241 aatttaggga agtggggaac tcagtagtat taactatttc aaaggagttt cttaaagaaa
      301 atgggttaaa tcccggagat acggtttttt tagatgaaga aaaacttcga gcggcaatta
      361 caaaaaaaca agccttatct gatgaaattg atttagcaat atctcaagca ttaactgaat
      421 atgatactac ttttagagag cttgtcgata aatgagtatt cattacctag aagccaaaga
      481 tattataaaa atgaatgtaa tccaaataaa aaaatactct cctaatgaac cccttggtgt
      541 aaaggatgcg aatgctttag aaatgtgtgt agggcaactt cgagcctcag cttttggtga
      601 agaggtttat ccttcgattt atgaaaaagc agctatccta cttatacaat tgataaagaa
      661 acatcctttt cacaatgcaa ataaacgtac tgctttctta gctacttttg tctttttgaa
      721 aatcaatgga cagcttttag ctattgatca aaaagatgct gtaaatttag cggtatatat
      781 tgctacgtat gataaggatt tcgatcaact aaaatatgaa gtgatcaaca ctataaagac
      841 acatacgcag cccatttaag gagttactgt atgtgtcttt atagggttat tagctgctat
      901 agcgaaataa ttgaagtaaa ttacaaatag ctataattca aatccaacta tatagaaaac
      961 gtaaattttc tatatagttg gattaagata acgactattt attttatcat atttttacta
     1021 taccctatac ttttacttgg tatattta
//
LOCUS       DACDIE010000196         1032 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.203, whole genome shotgun sequence.
ACCESSION   DACDIE010000196 DACDIE010000000
VERSION     DACDIE010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.203"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..493
                     /locus_tag="IV250_002913"
     CDS             <1..493
                     /locus_tag="IV250_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP8770131.1"
                     /translation="QFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIADWAIVV
                     LQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQAQEIYG
                     EHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEEHENWYA
                     EVE"
     gene            494..>1032
                     /locus_tag="IV250_002914"
     CDS             494..>1032
                     /locus_tag="IV250_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770132.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNN"
BASE COUNT          399 a          151 c          218 g          264 t
ORIGIN      
        1 ccaattcaaa tatatgaaag aaaagctaga accattattt gatgagtttg acgtcatcta
       61 tttagatgtc ccaccttcta tttctgtata tagtaaatcc gcgatgtaca ttgcggattg
      121 ggcaatcgtc gtcttacaga cccaagtaaa atcgatgaga aatgcgatgc aatatcttga
      181 gtatatggac ttttttgtga aagaatttga tacaaattta tatgttgctg gtgttattcc
      241 tttcatgttg gaaagcggtg acgcagtaga tcaggaaatg tataaacaag cccaagagat
      301 ttatggggaa catttgatta aaaatgttgt actcaaaaat gcgcgcttaa aacgatacga
      361 cggatcaggt attacgatgg agaagacaaa gaaaggtctc ttaaaacaat gggatcgcaa
      421 aagccacgaa ttatttatca gtattttgaa agaattggaa gaacacgaga attggtatgc
      481 ggaggtggag taattggctg acatcaaagg acttgatatt tttgggaaca acaaaggact
      541 cgccaaaata aaaaaagaag cagaagcaaa gaaaaagcag caagcaatta caccatctga
      601 aaataagcaa ttagaagtgc accaagttca agaaataaaa acttcctcca atgttgaaag
      661 acagagtaga ggaagaacaa aaaaagttgg cgcaccaatc agaaagtttg ataaggttta
      721 ttccaccaag catcctttaa aactgtcgac tttgttaaat gcgacttcca gagttttagt
      781 agaaaagtat gagactaata tgacaagaga tgaattgctt agaaaagctt tagatgagta
      841 tattaaacag aatcttacgc aagaagataa gcaagatttg tttaatgacg tggtgaaaga
      901 actagacttg ttcagagaaa aatatccaat tgttccagaa ttagatgatc gtggagaaat
      961 aataagaact accagtcaga taaaacaaga taccgaagac tcactgcgca aagggtgggg
     1021 aatgaataat aa
//
LOCUS       DACDIE010000197         1031 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.204, whole genome shotgun sequence.
ACCESSION   DACDIE010000197 DACDIE010000000
VERSION     DACDIE010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.204"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(37..1031)
                     /locus_tag="IV250_002915"
                     /pseudo
     CDS             complement(37..1031)
                     /locus_tag="IV250_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
BASE COUNT          261 a          211 c          188 g          371 t
ORIGIN      
        1 aatccactcc ttttttcatg ctctaaaatg atatttttac acaactgtta cggttgtttt
       61 acgaaattgg acgggacttt gccagtttaa tttctgtttt atccgttcat tattgtagta
      121 atagatatag ctatcgatgg ctgattttaa ttcctcgaaa cagttataaa ctttcccgtg
      181 aaaaatctct tgttttaata agccaaaaaa attctccata agtgaattat ctaagcagtt
      241 ccctttccga gacatgcttt gaaagatttt gtactccttc aatttgtttc tataagcttt
      301 catttgatac gcccaacctt gatctgagtg aaacgtacga cgatagggac aatcgttagt
      361 ggtctggata gcttcctcca aaccttccat taccgctaat gcattgggct tttcagagat
      421 acgataggat aaaatctcac tattgaacat atccataaaa ggatctagat acaatttctt
      481 ttgacggata atgcctttgg catccacttc atggtattta aattcagttg tatccgtcgt
      541 gattttctga tggcagatac tggtgtagaa acgacgatgg atccgatttt tggctactgc
      601 tccgacttgt cctcgataag aattgtagcg ccgtgattta cgtgtgtatg tcgttacttt
      661 tattcctagc ttcttgatca ggcgctgaac tttcttttta ttgatagaga aaccgcggtt
      721 tcttagttca ttggtcatcc gtaaacagcc ataatccttg tgcttctccc gaactttaag
      781 catttccgct tcaatctctt gatctggatt tttacggtcc aatcgttttt gccagtacat
      841 aaagattgcc ttagggaact ttaaagtttc tagaagctca actaatttaa acggtcctcg
      901 gaggcttgcg atgattcgtg cgattgcttc attcgtcgtt gttgggcttc ctgttttcgc
      961 agcttcctca attcttttaa aaaggcattt tcaatttgaa gggaacggac tcgtttctcc
     1021 aactcctcga t
//
LOCUS       DACDIE010000198         1026 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.205, whole genome shotgun sequence.
ACCESSION   DACDIE010000198 DACDIE010000000
VERSION     DACDIE010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.205"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            369..755
                     /locus_tag="IV250_002916"
     CDS             369..755
                     /locus_tag="IV250_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP8770133.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            849..>1026
                     /locus_tag="IV250_002917"
     CDS             849..>1026
                     /locus_tag="IV250_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8770134.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKF"
BASE COUNT          381 a          153 c          160 g          332 t
ORIGIN      
        1 ctattcgaac tcttttctag ttgttaatta tgaaggggga agaaataaaa aatacaatct
       61 atacacgaat aagaatatta tccaaaagga ggttttagaa aagatttatt atacataaaa
      121 agctcaagac attttcttag ccataaggac atgtttgata tttaagaaga tatatgaatt
      181 ttcttttcta agtataaaga taactatcca ttatcaacta aaaaaacatt tttgaaaata
      241 aacatcagca gtcctatcaa tgcatgtgag cttatagaag ttattcgcag cgcagcataa
      301 ttgcattagc ttcttttcgt gttttttgcc tcttttccct ttaaatctta atggaggagg
      361 tgtgcaatat gtcagataac gacgaacgcg taaatgccca gttgattcga ttctttgaaa
      421 aggtcatttt gaacaccgct aatacttatt ataaaaaaca agaagaaatt caaaaacatg
      481 aacaacttga tcaactccca ccatatttgt ttactaaaga tactataaaa tttaaacaac
      541 cgattaatgt tttaaatgtg actctccttg ttgaagatat aaaattagca aaaatccttc
      601 ctttgttgaa agaacctgaa caaatttttc ttatcgaaaa atttatcttt gataaaactg
      661 ataaagaaat tggaaaggtt ctggggatta caagacaggg tgcgacaaat ttaaaacatc
      721 gactttacaa aaaaattcaa aaaatgctgg tttaatatct gcataataac taattttgta
      781 tgcttctttg ctcgttctcc tccttcccaa agaaatagat tataagtcca tgggaagaag
      841 gtagatttat gaattcttta aaagaagatt tcattttagc aaaggctgga aatgaagaag
      901 ctgttgaggc aattctcaaa agatttagct ctttgattca taagcaatcc tggagaactg
      961 gaaaatatga tcaagattgt tatcaagaat gtatgcttgc aatatatcta gctatctcga
     1021 aattcg
//
LOCUS       DACDIE010000199         1022 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.206, whole genome shotgun sequence.
ACCESSION   DACDIE010000199 DACDIE010000000
VERSION     DACDIE010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1022
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.206"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            133..345
                     /locus_tag="IV250_002918"
     CDS             133..345
                     /locus_tag="IV250_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770135.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            507..>1022
                     /locus_tag="IV250_002919"
     CDS             507..>1022
                     /locus_tag="IV250_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HAP8770136.1"
                     /translation="MNKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVLDILNMYRDITFSKNKLKDINSIDNYYIQFKGFDFNSSTEF
                     KLALYAQFFIEKLNRFQEIVEDSDFNTFNSHKPMRGTYIKFLENYNDLKSTNNYQFGN
                     LSYEMISKVLSL"
BASE COUNT          383 a          133 c          162 g          344 t
ORIGIN      
        1 aaaaacacta tatattccga aaagtgagta tctccattca taaaataatc aacatattta
       61 aaaaatctaa ttagcttttt tagtcaatct aaagttcttt attctatgat ataatgttac
      121 tgtagaaagt ttgtggcggt ttggcaaatc caattgaagg agcgtggtgc ctatgggctt
      181 attagcttat cggacgtact ctgtagaaag gaaatgcctg atttgttctc attggatcaa
      241 atcattgcta ttatcggagc agcagcagta gttattactg ccgtagcgaa agctagcgat
      301 tcgattttag cttggttaaa gttcaaaaaa gaacaaaaaa aatagagccg tctcaacttt
      361 tggcgaggta gacgagctct aaagctttat ttacatgcca tccgctttga acggactaca
      421 ctgggcatgt gacagcatgc cctttcattt atattatatc tcattctttt atataatgaa
      481 agtaaataaa tcatggagga taaaatatga ataaatttaa tagattaaca ttaataaatc
      541 agtttgaaat tttaaaagct ttaaatccta acgaagaaaa atactatgct gaaaaaatag
      601 aaatactaac agaaggttac gaatatcact atagtgaaat atttgaaaat atttctgatc
      661 cattgccaga agaagattcg cggtttgttc tagacatatt aaatatgtat agagatatta
      721 cttttagtaa aaataaatta aaagatataa attcaattga taattattat atacaattta
      781 aaggttttga ctttaatagc tctaccgaat ttaagttagc actttatgca caatttttta
      841 ttgaaaaatt aaataggttt caagaaatag ttgaagatag tgattttaac acttttaata
      901 gccataaacc gatgagaggt acttatataa aatttctcga aaattataat gatttaaaat
      961 ctaccaataa ttatcaattt ggaaacttgt cttatgagat gataagtaaa gttctaagtt
     1021 tg
//
LOCUS       DACDIE010000200          993 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.207, whole genome shotgun sequence.
ACCESSION   DACDIE010000200 DACDIE010000000
VERSION     DACDIE010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.207"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>993
                     /locus_tag="IV250_002920"
     CDS             <1..>993
                     /locus_tag="IV250_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP8770137.1"
                     /translation="KKLNTLVTIENPFRVKQFSRMFDENKTDRNDALRIADFLRIQRF
                     TTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHFLENLTYKCNTLAREMRDESTSLFS
                     ATLISLMTEDFTLDELAELPLEAFCDLLQEKGKGRFKQPEKIAKAIQRAITMSYRLGA
                     LAQESINVVLSVLVREIRALEKNIKELDKAIEQVIVVIPEYQCLTSIPGVGKVYAAGL
                     IAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQSQNTPLTKQGNRYFRYYLVEAANSV
                     KNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKFTRLVDTLLRKHQLYTPPRSV"
BASE COUNT          352 a          196 c          178 g          267 t
ORIGIN      
        1 taaagaagct caacacactt gtaacgattg aaaatccttt tcgggtgaaa caatttagtc
       61 gcatgtttga tgagaataaa actgatcgaa acgatgcatt aagaatcgct gatttcttgc
      121 gtattcaacg attcacaact tcccctatca aagaagagaa atacatggcc ttacagcgtt
      181 taacacgtac tcgctatcaa cttattaagc aattgattcg tacaaagcaa cattttcttg
      241 aaaatctaac gtacaaatgt aatacgcttg ctcgagaaat gcgagacgaa tcgacgagtc
      301 tttttagtgc aaccctcatt tcactcatga cggaagattt cacattggat gaacttgctg
      361 agcttccttt agaagctttc tgtgatttac ttcaagaaaa aggaaaaggc cgtttcaagc
      421 aaccagaaaa aatcgctaaa gcaattcaac gtgcaattac catgagttac cgcctaggtg
      481 ctcttgcaca agaatcaatt aatgttgttt taagtgttct cgtacgagaa atacgagcac
      541 ttgaaaaaaa tatcaaagaa ttagataaag ccatcgaaca agtaattgtt gtcattccag
      601 aatatcaatg cttaaccagt attccaggcg ttggtaaagt ctatgctgcc ggtttaatcg
      661 ctgaaattgg tcaaatcgaa agatttgaag atcagacaaa attggcaaaa tacgctggat
      721 taagttggaa agtgaaccaa tctggaaact atcagtcgca aaatacacct ctgacaaaac
      781 aaggaaatcg atattttaga tactacttag ttgaagctgc caactctgta aaaaattatc
      841 ttcctgaata caaagctttt tatcaaagta aatataagga ggttccaaag catcaacaca
      901 aacgtgcact cgtcctaacc gcaagaaaat ttacgcgtct ggtggatacg ctactacgta
      961 aacaccaact ctatacgcca ccaaggagtg tga
//
LOCUS       DACDIE010000201          971 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.209, whole genome shotgun sequence.
ACCESSION   DACDIE010000201 DACDIE010000000
VERSION     DACDIE010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..971
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.209"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(1..971)
                     /locus_tag="IV250_002921"
                     /pseudo
     CDS             complement(1..971)
                     /locus_tag="IV250_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          247 a          200 c          179 g          345 t
ORIGIN      
        1 ttggacaaga ctttgccagt ttaatttctg ttttatccgt tcattattgt agtaatagat
       61 atagctatca atggctgatt ttaattcctc gaaacagtta taaactttcc cgtgaaaaat
      121 ctcttgtttt aataagccaa aaaaattctc cataagtgaa ttatctaagc agttcccttt
      181 ccgagacatg ctttgaaaga ttttgtactc cttcaatttg tttctataag ctttcatttg
      241 atacgcccaa ccttgatcta agtgaaacat acgacgatag ggacaatcgt tagtggtctg
      301 gacagcttcc tccaaacctt ccattaccgc taatgcattg ggcttttcag agatacgata
      361 ggataaaatc tcactattga acatatccat aaaaggatct agatacaatt tcttttgacg
      421 gataatgcct ttggcatccg cttcatggta tttaaattca gttgtatccg tcgtgatttt
      481 ctgatggcag atactggtgt agaaacgacg atggatccga tttttggcta ctgctccaac
      541 ttgtcctcga taagaattgt agcgacgtga tttacgtgtg tatgccgtta cttttattcc
      601 tagcttcttg atcaggcgct gaactttctt tttattgata gagaaaccgc ggtttcttag
      661 ttcattggtc atccgtaaac agccataatc cttgtgcttc tcccgaattt taagcatttc
      721 cgcttcaatc tcttgatctg gatttttacg gtccaatcgt ttttgccagt acataaaggt
      781 tgccttaggg aactttaaag tttctagaag ctcaactaat ttaaacggtc ctcggaggct
      841 tgcgatgatt cgtgcgattg cttcattcgt cgttgttggg cttcctgttt tcgcagcttc
      901 ctcaattctt ttaaaaaggc attttcaatt tgaagggaac ggactcgttt ctccaactcc
      961 tcgatgcgtt c
//
LOCUS       DACDIE010000202          964 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.210, whole genome shotgun sequence.
ACCESSION   DACDIE010000202 DACDIE010000000
VERSION     DACDIE010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..964
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.210"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(143..>964)
                     /locus_tag="IV250_002922"
     CDS             complement(143..>964)
                     /locus_tag="IV250_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HAP8770138.1"
                     /translation="SFRTSHYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDD
                     NVEKHNTWKEIRTENSHKTVIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLV
                     ELTKELDPQKRPVTIVTYLKSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLK
                     KELDGWMERCPDTPIMFTEYGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENF
                     VGEQVWNFADFATSQGILRVQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
BASE COUNT          303 a          195 c          124 g          342 t
ORIGIN      
        1 gaaagcccct ttttttcgaa aaaactagaa aaaaaacata aaaatattta tcattcttta
       61 aacagaaaat ctttctataa aaaacaaaaa aaaacgatta ggctaggcta atcgtttttt
      121 gaacattctt aaaaaatcca aattattttt tataactgaa attgggtatt gatgaccatc
      181 gtttctgtag atagtaagca atcattttag gttttctatc tctagtaaag attcctttct
      241 tatttccttg cactcttaaa atcccttgac ttgttgcaaa atctgcaaaa ttccacactt
      301 gttcaccaac aaaattttca catgtatcta ttacttcgtg atttgcttcg taataagcca
      361 cttgatactc ttctgtaaac attgttggta ctgtatcatg aaatccagct actgtatctg
      421 ctccatattc tgtaaacatt attggcgtat caggacatct ttccatccat ccatctagct
      481 cttttttcaa cagatatttt gcatgtgcca gatcccctcc cgaaacatac caaccataat
      541 aacgatttaa acataaaaca tcgacaatat ctcctacctt acaaacatct ggcgttgatt
      601 ttagataagt aacgattgtt acaggtcttt tctgcggatc aagttcttta gttaattcaa
      661 ctaaaggttc aaaatattct ttagcacctt cggaatccga atctggttca tttgcaatag
      721 accacattac aacacttggg tggttcttgt cgcgttctat caattccttg atcaccgttt
      781 tatgagaatt ttctgttcgt atctctttcc acgtattatg tttttccaca ttatcatcta
      841 ataaagtggc catgaaattt aaatgtaacc ctactgcagg tacttcatca ataactacaa
      901 taccttctcg atctgctaat cgcataattt cttctgagta aggataatgt gatgttctaa
      961 aaga
//
LOCUS       DACDIE010000203          947 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.211, whole genome shotgun sequence.
ACCESSION   DACDIE010000203 DACDIE010000000
VERSION     DACDIE010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.211"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>947
                     /locus_tag="IV250_002923"
     CDS             <1..>947
                     /locus_tag="IV250_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770139.1"
                     /translation="SGKSLEALELEQEEIRRFQNCSLYPVYHGSAKSNIGIEQLIEVI
                     TNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILHLRDSVRISEKEKIKIT
                     EMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLPQRERIENPLPLLQTTV
                     EPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLGKVQMEVISALLQEKYH
                     VEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLPLGSGMQYESSVSL
                     GYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGL"
BASE COUNT          323 a          151 c          202 g          271 t
ORIGIN      
        1 gtccggtaaa tcattagaag cattagaact cgaacaagag gaaatcagaa gatttcagaa
       61 ttgctccttg taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct
      121 tatagaagtg ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg
      181 cggaaatgtc ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct
      241 ttatggcgga atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa
      301 aattacagaa atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc
      361 cggggaaatt gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac
      421 aaagctattg ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt
      481 tgaaccgagc aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga
      541 cagtgacccg cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt
      601 cttagggaaa gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga
      661 gatagaacta aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata
      721 taccattcac atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc
      781 accgcttccg ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa
      841 tcaatcattt caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata
      901 tggttggaat gtgacggatt gtaaaatctg ttttaagtat ggcttat
//
LOCUS       DACDIE010000204          943 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.212, whole genome shotgun sequence.
ACCESSION   DACDIE010000204 DACDIE010000000
VERSION     DACDIE010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.212"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            2..>943
                     /locus_tag="IV250_002924"
     rRNA            2..>943
                     /locus_tag="IV250_002924"
                     /product="23S ribosomal RNA"
BASE COUNT          293 a          187 c          274 g          189 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact agg
//
LOCUS       DACDIE010000205          943 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.213, whole genome shotgun sequence.
ACCESSION   DACDIE010000205 DACDIE010000000
VERSION     DACDIE010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.213"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..157
                     /locus_tag="IV250_002925"
     CDS             <1..157
                     /locus_tag="IV250_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP8770140.1"
                     /translation="YDNGFNGYIRPDHGRMIWGETGRPGYGLYDRALGAVYLNGIWEA
                     LEKGASK"
     gene            231..>943
                     /locus_tag="IV250_002926"
     CDS             231..>943
                     /locus_tag="IV250_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAP8770141.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLA"
BASE COUNT          317 a          134 c          207 g          285 t
ORIGIN      
        1 gtacgataat ggatttaatg gctatattcg tccagatcat ggtcggatga tttggggaga
       61 aaccggtaga cctggttatg gattatatga ccgagctcta ggtgcagttt atttaaatgg
      121 tatttgggaa gcgcttgaaa agggggcgag taaataatta tataagggag tttatttaga
      181 ggaaaaattt tctcttctaa aaactaacgg aaggaaagga gtgtttaaat atgtttttag
      241 ataagaactt tttattagag aatgaaatag ctaaaagact ttttcataaa tttgcaaaac
      301 accaaccaat tatcgactat cattgtcatt tagacccagc atatttgcta gaaaataagc
      361 aatacaagaa tttgactcag gtatggttaa atgataaagg agctggagat cattataaat
      421 ggcgattgat gcgagctaat ggtatagaag aagagttgat cagtggtcca ggtgatgatt
      481 atgaaaaatt tttagctttt gtaaaaacag tggagaaggc gccaggtaat cctatttttg
      541 aatggtctca tttggaatta caacgttact ttgatattga tttgacaatt tctgagaaga
      601 atgcaccaga aatttggcga cgtgctaacg aaaaaattca aacggattcc tatcgcccga
      661 aagaattgtt ggagatgatg gatgtgaaag ccatctgtac aacggatgat cctacagatg
      721 acttgtggta tcatactgag ctttctaaac aggataattc tttcaaggta ttacctactt
      781 tccgtccaga taaggtctgg agtatcaaat catcaggctt ttcgaactat attttaaaac
      841 ttagtcaaac agcggctata ccaatcaaag actttaatgg gttgaagcaa gcattgagcc
      901 aacgagtgga tcattttcat caaattggct gtcgattagc aga
//
LOCUS       DACDIE010000206          938 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.214, whole genome shotgun sequence.
ACCESSION   DACDIE010000206 DACDIE010000000
VERSION     DACDIE010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..938
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.214"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..531
                     /locus_tag="IV250_002927"
     CDS             <1..531
                     /locus_tag="IV250_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770142.1"
                     /translation="FPKLYSYNMFGFVMNILKYSEYESAQIGNDNGSLRSTILPFAQS
                     DVVNTVVQGVDPQITNYLSSQVDSFDDNGKNTYINIIKNISEFQQNQFIFPLLNMIAL
                     LPVEETAIIAETLLNLTSFKRKYTTSVETVGGPIDVLAITPNDGPIWIKRKHYFDIDN
                     NIGYRLRKERANDYNN"
BASE COUNT          349 a          127 c          141 g          321 t
ORIGIN      
        1 ttccctaaac tatattctta taatatgttt ggatttgtta tgaatattct gaaatactca
       61 gaatatgaaa gtgctcaaat aggaaatgac aatggttcat taagatctac tatattacca
      121 tttgctcagt ctgatgtggt aaatactgta gttcaagggg tagatccaca aattactaat
      181 tatctgtcga gtcaagtcga tagttttgac gataacggaa aaaataccta tattaatatt
      241 ataaagaata tttcagagtt tcaacaaaat caattcatat ttccgttact aaacatgatt
      301 gctttactcc cagttgaaga aacagcaatt atcgcagaga ctttattaaa tttaaccagt
      361 tttaaacgaa aatatactac ttctgtagaa acagtaggtg gcccaattga tgttttagct
      421 attacaccta atgatggccc tatttggatt aaaagaaaac attattttga tattgataat
      481 aatattggat acagattgcg aaaggagaga gcgaatgatt acaataatta aacctcagga
      541 aaaatcccct gttttgaaat ttaatgagag caatctagcc cataataaaa tgagtataac
      601 tgatacagta tttgatgtaa tggttgagca acaaaaaaaa attaataata acaatagaca
      661 aagaaaagaa attaaccaat attaatgttt tttctgttgc gttttttgca gtcataattt
      721 aaaacctcgc tcaatcttgg gcgaggtctt tttttatttt tctgaaaagt taaagtaaat
      781 aatagcataa aataatatat tttacaaaga ataagtacaa tctagttttt tgctattaaa
      841 tgtgtaataa taaatatgcc atcacaacat gaagaatgaa atccattatt atctagtcta
      901 gtccattctt tttgtttgca gtatttgtga tggctttc
//
LOCUS       DACDIE010000207          925 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.215, whole genome shotgun sequence.
ACCESSION   DACDIE010000207 DACDIE010000000
VERSION     DACDIE010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.215"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            192..893
                     /locus_tag="IV250_002928"
     CDS             192..893
                     /locus_tag="IV250_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP8770143.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
BASE COUNT          333 a          161 c          149 g          282 t
ORIGIN      
        1 ttgttgtttt ttatacacaa atcattcgct tcaaaacaaa ttattcaata ccattgtata
       61 ttttttacat aataacaaat tatatttgta aagtattaaa acttgtttct aatgttggta
      121 tgttaaaact ttacaaattc tttaaaaaaa gtgacaataa atatagtgaa caataaggag
      181 aggacttttt aatgcgtgtt aataaaatcg attttcactt ttcaaaactc agatatcaac
      241 ctgattttga acatttctcg gatgaagagt tccaacagat caaggaacat actgttcttc
      301 gttcttataa aaaaggacag atccttttcg acgatggtga tcccagacaa cggttgtata
      361 tcttgaccaa aggactcgtt cgtttagaac gttatgatga aagtgcaact tattactact
      421 atgactatgt gaatacaaat aacctttttc ctatgtcagg aatgtttgga gaagagacct
      481 acacttatac agctcaagcg atgactgata ttgaaacatt ctaccttcct gtttcctact
      541 atgaaaaatt agcacgaaaa aatcccgaac agttgatcta tatgatacaa aaaatttcta
      601 aagtagttga aatacatgaa ttgcgtatcc aaatcggatt gacctctagc gcctttgacc
      661 gggtaaaaca aaatttattc attttgaaag aagaattggg cgtaccaaca aaagatggtc
      721 atatcaccat tccttatccg atcaccttaa aagaattagc agtgaatagc ggcacgacac
      781 gtgaaactgc tggacaagta atcaaacaac tacgtgattc cgggctgtta gattatgaca
      841 aaaagcgttt tactttctgt aagacagcta ctactccagc tatgagagaa taacgcgaac
      901 aaaaataagc agtgaccctg ataaa
//
LOCUS       DACDIE010000208          924 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.216, whole genome shotgun sequence.
ACCESSION   DACDIE010000208 DACDIE010000000
VERSION     DACDIE010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..924
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.216"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>924
                     /locus_tag="IV250_002929"
     CDS             <1..>924
                     /locus_tag="IV250_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HAP8770144.1"
                     /translation="DSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHTCR
                     NCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQSY
                     FVQPRHSIANHVRYKIASLLTEKVSLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSKRI
                     LPRVLMVDEFRSHASIEDKMSFICADGETGQLIDVLPTRKLPRLTSYFLGCANPEEVK
                     FLVTDMNAAYFQLTKRILPNAKVVIDRFHIIKHMNQAFNELRIREMNELRKVGQKSQA
                     EKLKKNWRFLLKNRANINHYEYKTWKSFRAP"
BASE COUNT          311 a          171 c          181 g          261 t
ORIGIN      
        1 tgattctatc aaaaaaatgc tgcgattaat agagaaagat ttgatgatta cagaggtctc
       61 ttacgagacc tttcagaaga aaaagacgtt gatcgtcgat gctgttttct cacctgctcc
      121 ccatacctgt agaaattgtg gttctactgt ggtagatgga aacgggaaag taattgtagt
      181 gaaaaatggg aagaaggaaa cgattgtccg ctttgaacaa tacaatcaca tgcctttggt
      241 tatgcgctta aaaaagcagc gctatacctg taaaaactgt cgaacccatt ggacggctca
      301 aagttatttt gttcaaccca gacattcaat cgcgaatcat gtcagatata aaattgcttc
      361 tttactgact gaaaaagtat ctttatcttt tattgcgaaa aattgtcagg tatctttgac
      421 caccgttatt cgtacgttga aagagtttaa aagctactta ccaaagcaat ctaagaggat
      481 tctccccaga gtattgatgg ttgatgaatt tcgttcgcac gcttccatag aagataaaat
      541 gagctttatt tgcgcagatg gcgaaacagg acaattaatt gatgtcttgc ctacgcgtaa
      601 attgcctcga ttaacaagct atttcttagg ctgcgctaat cctgaagaag taaaattttt
      661 agtcacagac atgaacgccg cctacttcca gcttaccaaa cgtattctgc caaatgcgaa
      721 agtagtgatt gatcggtttc atattatcaa acacatgaat caagccttta atgagttgcg
      781 tatccgtgaa atgaatgaac ttcgtaaagt aggacagaaa agtcaggcag aaaaactgaa
      841 aaagaactgg cgctttttgc taaaaaatcg tgcaaacatc aaccattatg aatacaaaac
      901 atggaaaagt tttcgagcac caaa
//
LOCUS       DACDIE010000209          911 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.217, whole genome shotgun sequence.
ACCESSION   DACDIE010000209 DACDIE010000000
VERSION     DACDIE010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..911
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.217"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..312)
                     /locus_tag="IV250_002930"
     CDS             complement(<1..312)
                     /locus_tag="IV250_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770145.1"
                     /translation="MFILDDEIKKVKNPRIIPLLEEVRSSFYQGNYRSAIAVNYSALI
                     LDLIDKLSDLANIYEDNTSKKILEDIARLRKSDPNSPKWEMELLEKAYSSTELIDSYE
                     YE"
     gene            434..>911
                     /locus_tag="IV250_002931"
     CDS             434..>911
                     /locus_tag="IV250_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770146.1"
                     /translation="MDLKQSFYIWLYPDNPMSIMADIFVVSILLYLFYLIFFHNLIGY
                     MILDWKGMFDIERKPFANNQTYTVFESGDWEENEVDVIHEIGLALSGIQDADVLVSMT
                     TDTGYNAYEVFYLMYFIWRDGQEIINGRAEKTINSNTEISFCMGEGEMIVYKDGSAN"
BASE COUNT          309 a          120 c          145 g          337 t
ORIGIN      
        1 ctcatattca tagctatcaa ttagctcagt tgaactataa gctttttcta gtagttccat
       61 ttcccatttt ggagagtttg gatcactttt tcttagtcta gctatatctt ctaaaatttt
      121 cttagaagta ttatcctcgt aaatatttgc taaatcagat aatttatcaa ttaaatctaa
      181 aataagcgca ctgtaattga cagctattgc agaacgataa tttccctgat aaaaactact
      241 tctaacttct tctaataatg gaataattct aggattttta acttttttta tttcatcatc
      301 taatataaac atataaatct ccttttttta tttgtatgac aagtttacca taaaaaaaaa
      361 ttaaaaagcg agacatattt atgttgttgt gttatcataa taacataaat atgtagagtg
      421 agatgatgaa aaaatggatt tgaaacaatc gttttacatc tggttatatc ctgataatcc
      481 tatgtcaata atggctgata tttttgtagt tagcatctta ttgtatttat tttatctaat
      541 tttctttcac aatttaatcg ggtacatgat tcttgattgg aaaggaatgt tcgatataga
      601 aagaaaacca tttgctaaca atcaaaccta taccgtcttc gaaagtggag attgggaaga
      661 aaatgaagtt gatgtgatcc atgaaattgg attagcttta tcaggaatac aagatgctga
      721 tgttttggta tcaatgacaa cggatactgg atacaacgct tatgaggtat tttatcttat
      781 gtattttata tggcgtgatg gtcaagaaat tatcaatggt cgtgctgaaa aaacaatcaa
      841 ttctaatact gaaatttctt tttgtatggg tgaaggggaa atgattgtat ataaagatgg
      901 gtcagcaaac a
//
LOCUS       DACDIE010000210          906 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.218, whole genome shotgun sequence.
ACCESSION   DACDIE010000210 DACDIE010000000
VERSION     DACDIE010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..906
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.218"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..521
                     /locus_tag="IV250_002932"
     CDS             <1..521
                     /locus_tag="IV250_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HAP8770147.1"
                     /translation="LISEYKNEIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLK
                     KQVEKLSHKSYKDVYDEYSKETDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVT
                     DEIDEHRGELIKNLVTNRAKIQTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRG
                     IKNLVKSYLKMN"
     gene            524..>906
                     /locus_tag="IV250_002933"
     CDS             524..>906
                     /locus_tag="IV250_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HAP8770148.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNFYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSG"
BASE COUNT          372 a           99 c          177 g          258 t
ORIGIN      
        1 atttgatttc agagtataaa aatgagattg aaacatgtgt aaacgaaata agaaaattaa
       61 attgggatat tgtttctgtg aaaaaagaaa tacaagataa ggcgaaggta gagcttgaaa
      121 ttgaagattt aaaaaaacaa gtagaaaagc tatctcataa gagttataaa gatgtttatg
      181 atgagtattc aaaagagaca gatatttata gggaactgaa acgtgatgtg gaggcactta
      241 aggaaattcc caaaaatcta actgatagtg ttgacaaaat tgactttggc aatggtataa
      301 aagttacaga cgaaattgat gaacaccgcg gagaattgat taagaattta gtgaccaatc
      361 gggctaagat tcaaacaata attaatgaaa tgtcagatga aattgatagt tatagagcag
      421 agctgaataa atcagattgg aaagttaact ataatgaggt ttcaaagagg tatcaaagag
      481 gtatcaaaaa cttagtgaag agttatctca agatgaatta aaaatgttac aaaaccttga
      541 ttctttatat caatctttat caaaaactga agagcgattg gaagaaatta ttcaacaaga
      601 aataaagctt aaagattacc aagttgaggt tgactcagca atgtcaagaa tgaataaatc
      661 atatcaaaat ttctattctg agagaagtag ttcctcgcgc gaaatttttt ctgatattac
      721 aggaatagag gctactattc aaccaaatag ggatttcaat aattatgtta aagagctcag
      781 agcttgtatt aataaggagg acacttttga cagtgagttt ggatctctta ttgataagct
      841 gacaaaaaat tccattacta atcaagatat atataatgac atacttgata taagagttag
      901 tgggga
//
LOCUS       DACDIE010000211          879 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.219, whole genome shotgun sequence.
ACCESSION   DACDIE010000211 DACDIE010000000
VERSION     DACDIE010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..879
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.219"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..143
                     /locus_tag="IV250_002934"
     CDS             <1..143
                     /locus_tag="IV250_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP8770149.1"
                     /translation="VSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIP
                     KR"
     gene            258..>879
                     /locus_tag="IV250_002935"
     CDS             258..>879
                     /locus_tag="IV250_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770150.1"
                     /translation="MTAEIGIMNKSGIVLASDSASTIGDSKVYNTAKKLFTLDSMHSV
                     GIMIYGNAEFNGIPWEIIITQYKKSIGSSVFNTLEEYADNFIEFVKTASFIRSEQTEQ
                     EQMIGVFQKIISGLFESIEVDINFLISQGTQIDKDVLVKLLQTKMNTNLSQQSQTFIL
                     DIEKALFLSNYGEILKDILNSISTMEGVSEAISEEIQSYVYEIIIRD"
BASE COUNT          340 a          108 c          160 g          271 t
ORIGIN      
        1 atgtatcttt taacatgcgc aatttcccta ataagaaagt atacgtcatg atgcgggcaa
       61 cgaatgatcc agaaggaaac actaaaggcg gtgcgcaaga tttccatgac aaacgttggt
      121 atttaaatat tcctaaacga taaaaatagc tcctcgttga ggagcagtac atagaattga
      181 aaattaacgt taaatcaaaa aaatatttac aagatgttta tttaagtaga taataaaaga
      241 aaaaagcagg tgaaattgtg acagcagaaa ttggtattat gaataaaagt ggaatcgtgt
      301 tagcttcaga ttcagcatct acgattggag atagcaaagt atataatact gcaaaaaaat
      361 tatttacttt ggattccatg cattctgtag gtatcatgat atatggtaat gctgaattta
      421 atggtattcc ttgggaaata atcattactc aatataagaa aagcattggt agttctgttt
      481 ttaatacttt ggaagaatac gcggataatt ttatagaatt tgtcaaaaca gcatctttta
      541 ttagaagtga acagaccgag caagaacaaa tgattggggt ttttcaaaaa ataatttcag
      601 gtttatttga gagtatagaa gtagatatta actttttaat aagccaaggt acacaaatag
      661 acaaagatgt cctagtgaag ttattacaaa caaaaatgaa taccaatttg tctcaacagt
      721 cccagacatt cattttagat attgaaaagg cgttatttct tagtaactat ggagaaatat
      781 taaaagatat tcttaatagt atttctacaa tggaaggtgt ttcagaagct atatcagagg
      841 aaattcaaag ttacgtttat gaaattataa ttagagacg
//
LOCUS       DACDIE010000212          872 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.220, whole genome shotgun sequence.
ACCESSION   DACDIE010000212 DACDIE010000000
VERSION     DACDIE010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..872
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.220"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            370..872
                     /locus_tag="IV250_002936"
                     /pseudo
     CDS             370..872
                     /locus_tag="IV250_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          307 a          151 c          160 g          254 t
ORIGIN      
        1 gtttcaaagt tagtagagaa aatgtagtta tatatttaaa tttttaccag aaagaaatga
       61 acaccttcaa ttatctaaat ctcgaaaatg gttagatgta atcatcagga aaaacaatct
      121 taaacgaatt actattcatg gtcttagaca cacacatgct agcttgcttt tagcaagcta
      181 gtgcaaatat taaagatgtg caagagcgtt taggccactc ttctattcaa atcattatgg
      241 atctatatat tcatattaca gacaaaagaa aagaagaaac cgtagcgcaa ttcgcaaaat
      301 ataggctcta taatttatgg gactgatgaa tcaaaattga ttcttcagtc ccattgttat
      361 tttaggtata tgattcgtac attgaaagag ttgaaaaact atcttccgaa gccgtcaaaa
      421 aggaccctgc caaaagtatt gatggtggat gaatttcgtt cgcacgcttc aattgtggat
      481 aaaatgagct ttatttgtgc ggatggcgag actggaaaac tcattgatat tttgcctaca
      541 cgaaaattgt ctcgattaac acactatttt agtcgctgtg caaatcctga agcggtggaa
      601 tatttagtga cagatatgaa cgccgcctac ttccaactga caaaacgtgt cttaccaaat
      661 gcgaaagtcg tcattgattg ttttcatatc atcaaacatt tgaatcaggc gttcaatgat
      721 tttcgtgtac aagaaatgaa aagattacac cagttagggc aacgacggga agccgagaaa
      781 gtgaaaaaga actggcgctt tcttctaaaa aaccgtcaaa aaatcaatca ctatgattac
      841 aaaacatggc gcagttttcg tgcacctaaa ta
//
LOCUS       DACDIE010000213          870 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.221, whole genome shotgun sequence.
ACCESSION   DACDIE010000213 DACDIE010000000
VERSION     DACDIE010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..870
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.221"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..408)
                     /locus_tag="IV250_002937"
     CDS             complement(<1..408)
                     /locus_tag="IV250_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770151.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLID"
     gene            complement(425..>870)
                     /locus_tag="IV250_002938"
     CDS             complement(425..>870)
                     /locus_tag="IV250_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HAP8770152.1"
                     /translation="IQENELAVSLPEVLKVRDSGIVYYTKEIGKTKIEHLSPLISSVV
                     QFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMHITIYNYISKRKISTAKIMLKSN
                     HTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLGLVDNFNNIS"
BASE COUNT          302 a          154 c           96 g          318 t
ORIGIN      
        1 atctattaac ttgtagttgt ttaacttttc tgaaaaagtt agacccacgt aataatcttc
       61 ccagctttct ctttcaatta actgatccaa tttttgataa tccataaatt catcatgtat
      121 caaaaagaat aaataacttg atattttagc atctagctta atcagattaa ataatgtttg
      181 tttaaaattg ctaccattaa tatgactgat agattctcta gcttcttcac ataaactttt
      241 tactttatta attactactt tgtcatattc atttaacaca ctattattac cattacttag
      301 acgctcagat tctacgaaac aactcaaatc agaaatttcc caaaaaaatt ttttgttaaa
      361 atccaatttc ctcaactcct ccaaactacg taaatgatat agtctcataa taaaataaaa
      421 cctctcagct aatgttgttg aaattgtcca ctaaacctaa ttgatattgc ttaggagata
      481 cacctgcgta ctttttgaat actctcgaaa agtgtgaaga atctgaaaat cctaaaccta
      541 aagaaacttc actgatagta tggtttgatt tcaacattat tttagccgta gaaatttttc
      601 gcttagagat ataattataa atcgtaatat gcatttcatt cttaaagctt tctcttaatt
      661 tagtttcact tacagaaaaa taattagcta tttcctttac agtaattctt ttatagatat
      721 ttaagccgat gaattgaacg acagaagaaa ttaagggaga aagatgctct atcttagttt
      781 ttccgatttc cttagtataa taaacaatcc cagaatccct cacttttaag acctcaggta
      841 acgatacagc taattcgttt tcttgtatca
//
LOCUS       DACDIE010000214          860 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.222, whole genome shotgun sequence.
ACCESSION   DACDIE010000214 DACDIE010000000
VERSION     DACDIE010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..860
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.222"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>860
                     /locus_tag="IV250_002939"
     CDS             <1..>860
                     /locus_tag="IV250_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770153.1"
                     /translation="NYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNLCTTLVAYHY
                     GERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFYAYQIISGKE
                     EKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFIAIFKKLFSA
                     AGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNNDLTTYSMGL
                     VGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAKLLQFPWRIL
                     SLQIIFSSLI"
BASE COUNT          258 a          142 c          142 g          318 t
ORIGIN      
        1 aaattatttt tatccctttc tgactttttt tccagctgta atgttatatt ggatgacaaa
       61 aaatttgatt gtaagctata ttatttacgt ttggttattg aacttgtgta caacactcgt
      121 agcttatcat tatggcgagc ggtttttaaa acaaaaaaaa gcggcatttt tatttagttg
      181 tctctatatt ttctctgctt atcggacagt agacatctac tatcgttccg caattgccga
      241 agctatcgca ataacacttg ttatccctgt attattttat gcttatcaaa tcatctctgg
      301 aaaagaagaa aagtatccgt ctgtaaagct agcattgagt atgagtttgc tagtgtacag
      361 ccatgtacta tcaacattaa tgagtacagc attaattatc atttttattt ttattcgttt
      421 ggtttctaaa ggtttcaaga atgcagattt tattgctata tttaaaaaat tatttagtgc
      481 ggcaggaatg acattggttc tgacgagtgc tttttggtat ccgatgtttg aacaaatgct
      541 ctaccaaaaa atcaacaaac ctagcgtgac aaatttatat gctcatgcat ctaatgtgtt
      601 cgatagtctg actgaagcta tgaacaacga cttaactact tactcaatgg gactagttgg
      661 tctgttatct ttatgtattc ccctgatttt atttaaaaaa ttaacaaaca tagaaaaaaa
      721 gatttattat ggtacgtgtt tgacttggct agctacaact agtttggtgc catggtatct
      781 tctgcaaaat acgccagcca aattgttaca atttccttgg cggatcttaa gcttgcaaat
      841 aatattctct tctctgatat
//
LOCUS       DACDIE010000215          856 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.223, whole genome shotgun sequence.
ACCESSION   DACDIE010000215 DACDIE010000000
VERSION     DACDIE010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..856
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.223"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..698)
                     /locus_tag="IV250_002940"
     CDS             complement(<1..698)
                     /locus_tag="IV250_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008270028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP8770154.1"
                     /translation="MMGNLVLSYNIYYFFITFLTMVVSYFSMKQIKNNRYISLLFSII
                     YTFSAYRAIDIFHRASLGEAVALTFLPLILMGCYEIYIRDYQKWYWLSIGMTLVVYTH
                     LLSVAMVSVFIGGTLFLSFYFWDQKIARLLSLLKATVLTFFLSAGFLIPFIQQSRAQE
                     LKVPLGKELSGMAPSDMLTHILNNNYNNYTIGLFLFLGLIGAFIFIKKLTADDLFIFF
                     LGVFVLFCSTNLFP"
BASE COUNT          359 a          129 c          133 g          235 t
ORIGIN      
        1 caaggaaata aattggttga acaaaaaaga acaaatacac caagaaagaa aataaataaa
       61 tcatcagcag ttaatttttt tataaaaata aaagcaccaa tcaatccgag aaataagaat
      121 aatccaatag tatagttgtt atagttatta tttaaaatat gtgtcagcat atcactagga
      181 gccataccac ttaattcttt tcccagtggg actttcaatt cttgtgctct agattgttga
      241 ataaaaggaa ttaagaatcc cgcacttaaa aaaaaggtga ggacggtagc ttttaaaaga
      301 cttaagagtc tagcgatttt ttggtcccaa aaataaaaag aaagaaacaa cgttcctcca
      361 ataaatacag aaaccatagc gacagaaagt aaatgagtat acactactag cgtcatgcca
      421 atagacaacc agtaccattt ttggtaatct ctaatataaa tttcgtaaca ccccattagt
      481 atcaaaggca aaaatgttaa agcgacagct tctcccaatg aagcacgatg gaatatgtca
      541 atggcacgat aggcggaaaa tgtataaata atcgaaaata acagcgaaat ataacgattg
      601 ttttttattt gtttcataga aaaataagaa acgaccattg ttaaaaaagt gataaaaaaa
      661 tagtagatat tataacttaa aacaagattt cccatcattt tataaaataa aaaagctgga
      721 taaagagtaa gccatggata aaaaatattc atcattgttc catgatgatc gaagttattg
      781 aagtttactg gagaactcca tacattatct agtcctatga ttcgagataa atgaaagtaa
      841 gtatcctgat tgacac
//
LOCUS       DACDIE010000216          842 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.224, whole genome shotgun sequence.
ACCESSION   DACDIE010000216 DACDIE010000000
VERSION     DACDIE010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..842
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.224"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            67..>842
                     /gene="uxuA"
                     /locus_tag="IV250_002941"
     CDS             67..>842
                     /gene="uxuA"
                     /locus_tag="IV250_002941"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP8770155.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYE"
BASE COUNT          294 a          117 c          177 g          254 t
ORIGIN      
        1 agtaggtcct caagcagttc gtaaaacaat caataaatat ataacagaag ttggaggaaa
       61 gtaactatgg aaatgacttt tagatggtat ggggcaaatg aagaaaaaat ctcactacaa
      121 gaaattcgtc agatcccagc aatgaaagga attgttggaa cattaatgga tataccagcc
      181 ggagaggttt ggccgagaga acgaattaaa tctttaaaag atgaagtcga agccaatgga
      241 cttaccttga aagttattga gagtgttaat gttcatgatg atatcaaaat tggtttacca
      301 acacgagatc aatacattga gaattataaa cagacgattc gcaatttggc agaatttggg
      361 attgaggtta tctgttataa ttttatgcca gtatttgatt gggtaaaatc ggatttggat
      421 tatcgtttag atgatgggtc ttctacgtta gcctatattt caaaaaatat ccctactgat
      481 ccggaggaaa ttattagaga agtagaaaat tctactaata attttgaatt gccgggttgg
      541 gaacctcatc ggttatcaca aattaagcaa ttattagaag cctataaaga agttgatgaa
      601 gagaaattac gagagaactt tgcatatttt ctcaaagcaa ttatcccgac atgtgaagaa
      661 gttgggatta aaatggcagt tcacccagat gatccaccat attccatttt cggattacct
      721 cgtattgtta aaaatcgtga cgatttagat tggttgtgta atgtagtgga ttctccttct
      781 aatggtatta cattatgtac tggaagtatt gcagaacgac cagataatga tgtctatgaa
      841 at
//
LOCUS       DACDIE010000217          798 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.225, whole genome shotgun sequence.
ACCESSION   DACDIE010000217 DACDIE010000000
VERSION     DACDIE010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..798
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.225"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..>798)
                     /locus_tag="IV250_002942"
     CDS             complement(<1..>798)
                     /locus_tag="IV250_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8770156.1"
                     /translation="VTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNK"
BASE COUNT          294 a          134 c          121 g          249 t
ORIGIN      
        1 tttttatttg tcgcaaaaaa tgttacaaat aggatcacaa taaatattcc tgcgaataca
       61 aaaaatggag cagtcaaatt tgtacttctt gttagaaatt gtgctaattg catagcaata
      121 ggagctataa aattaccaat attacagcca ataaaaatca atgaagtagc aaaattaatt
      181 gtattctttg tagtgacttt atctaagtta ctaaagataa atgggaagca ccatgatcct
      241 gttatacttg ataaaaataa acccgttagc aggaacgtca tattaccgtt tgacacagca
      301 attaacaaat ttgaaattat aaagaatata attcccaagt atagggttcc ttttcctatc
      361 cacttattta ttggtccaaa cataaatcct gctagtattc ctaagatagg cattagagct
      421 aaatagttac tagcattaaa gtttacccct ttaatttcag ttgcaattga tgcaaatcgt
      481 acagaaatag caatactatt cattacgagt aagattgcaa atagaactag ggcaaaaacc
      541 attggattca tcttttcttt tacatgattt tcaggtatgt cactattttc atctctaatt
      601 tcaggaactt ttagcgcgaa taagagagct acaggaaacg caattgcata gactaaaaac
      661 gaagccgtcc aattaatgtt cactaaaact ccagcaagaa aaataaggat tgtctgacct
      721 aaagcttcca ttgaatttct aatacctaat agcgttgctc tagtattgcc agagtataaa
      781 gagctgatat atgtcaca
//
LOCUS       DACDIE010000218          776 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.226, whole genome shotgun sequence.
ACCESSION   DACDIE010000218 DACDIE010000000
VERSION     DACDIE010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..776
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.226"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>776
                     /locus_tag="IV250_002943"
     CDS             <1..>776
                     /locus_tag="IV250_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP8770157.1"
                     /translation="LEEEFDVTLTDDEVSLILIHFQIAIEDQSKANNIIIICQYGISS
                     AQLIYNKVRRFLPAKDNVEISTLDKFHLAEIGEVDLIISSLSFDAGGIPLVRVSPLVS
                     KTDYMNIMEAYTKYLIEEPSRFIQNELIPKAVKVPTISKFVQSSLVKTGVQVESKEEC
                     LDLMISELEANSLVTEEFRASIFSREKMGNTNLESGVALPHANPSMVIESNIFIVALK
                     NAINWGDRKVKLVIMVSLAEEDVSEIRGVYEELYQFIGQK"
BASE COUNT          262 a          107 c          147 g          260 t
ORIGIN      
        1 cttgaagaag aatttgatgt gactttaacg gacgatgaag tttccttaat tttgattcat
       61 tttcagattg ctattgaaga tcagtccaaa gcaaataata tcatcattat ttgccaatac
      121 ggtatttctt ctgcgcaatt aatttacaat aaagttcgac ggtttttacc agcaaaagat
      181 aatgtagaaa tatcaacttt agataagttt catttagctg aaattgggga agttgatttg
      241 attatttcct ctctttcctt tgatgcaggt ggtataccct tagtaagggt atctccattg
      301 gtaagtaaaa cagattatat gaatattatg gaagcttata ctaaatattt aatagaagaa
      361 ccttcgcgat ttattcaaaa tgaactgatt cctaaagcgg ttaaagttcc caccatatct
      421 aaatttgttc aatcatcttt agtaaaaaca ggagtacagg tggaaagcaa agaagaatgt
      481 ttggatttga tgatttccga actagaagcc aattcattag ttactgaaga gtttcgtgct
      541 tctatcttct ctagagagaa aatggggaat actaatttag aaagtggtgt tgctttaccg
      601 catgcgaatc catcaatggt cattgaatcc aacattttta ttgtagcatt aaaaaatgcg
      661 attaattggg gcgatcgaaa agtcaaatta gtcattatgg ttagtttagc tgaagaagat
      721 gtatctgaga ttagaggagt atatgaagaa ctttaccaat ttattgggca aaaaga
//
LOCUS       DACDIE010000219          762 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.227, whole genome shotgun sequence.
ACCESSION   DACDIE010000219 DACDIE010000000
VERSION     DACDIE010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..762
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.227"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            48..404
                     /locus_tag="IV250_002944"
     CDS             48..404
                     /locus_tag="IV250_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP8770158.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
     gene            394..660
                     /locus_tag="IV250_002945"
     CDS             394..660
                     /locus_tag="IV250_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8770159.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
BASE COUNT          302 a          102 c          157 g          201 t
ORIGIN      
        1 ttatgttata tactataata taaaatcaat atatgatata attgggtatg gaaaaaatat
       61 cttttgaaac atttaaaaga cctaatggtc atgatgaatt tttagaatgg ttggaaacat
      121 taccaaaaaa agatagtgcg aagcttttac gtacaataga agaaacagaa aagaatggga
      181 tgctagtagc tcaacggttg aaatgggtga aaaaattaga cactaatttg tacgaattaa
      241 gaagtaaggt aggatcaaac atccaaagag ctatctattt ccatgtggtc aatggaaggt
      301 atgtcattac ccatggattt actaaaaaaa cggagaaaac accaataagt gaacttaaac
      361 atgcaaaaga acttagagag gagtggttca caaatgatga ttaaaaaatt gattgaagaa
      421 aagcaaaaag acaaagattt tgcagaagca tatgaccgtg aaggaagaaa actcgaaact
      481 gcggttgctc tttatcatgc acgggaggaa gcaggcctaa ctcaagcgga gcttgcacaa
      541 aaagcacata caactcaatc tactattgct cgaattgaga gaggggataa cgtatctttc
      601 gataaacttg atgatatcgc tagggcatta ggcaaaaagc tggtagtaga atttgcttaa
      661 aagcgagttg aaaatatacc tgtaataggc tcctaactaa tcaattagtt tagtgggcct
      721 ttttgtacta aatagataaa agttgtccta tagtgtaaaa gt
//
LOCUS       DACDIE010000220          761 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.228, whole genome shotgun sequence.
ACCESSION   DACDIE010000220 DACDIE010000000
VERSION     DACDIE010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..761
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.228"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(593..736)
                     /locus_tag="IV250_002946"
     CDS             complement(593..736)
                     /locus_tag="IV250_002946"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770160.1"
                     /translation="MEKEAFDLHVLGTPPAFVLSQDQTLIKSSNNLAIVKLNLFASIL
                     LAC"
     gene            655..>761
                     /locus_tag="IV250_002947"
     rRNA            655..>761
                     /locus_tag="IV250_002947"
                     /product="16S ribosomal RNA"
BASE COUNT          313 a          124 c          134 g          190 t
ORIGIN      
        1 ccttaggcag cttttgctgt gtagaaagga ataaataata acgaaagttc aaaagctaat
       61 gccgactatt ctagacatta aagaagacga aaacgaacga taaagaaaga aaagttatga
      121 ataatcgttt attgaaaaaa acttgaaaaa atacaaaaaa ttcgttgacc aagctaaatt
      181 tagttggtat attattactt gtccaaaaga cagaagaaaa tatttattca aaaacataac
      241 aaaaaagtta ttgacatgtt actaacgaca tgataagata actgagtcgt taaaaaagaa
      301 ccacttgcga agcttgattc tatcgaaaaa aacttcaaaa aagttcttga caaataacaa
      361 acgataagtt atgataaatg agttgccgaa agatttcgaa aaacgaaatc gaaaaaaact
      421 tttaaaaaag agttgacaat cgcttgtctc tctgatatga tataaaagtt gctacggcag
      481 ccaaaaatga atcgggaaac actctcgatg aagtagacct ttgaaaactg aacaaagtaa
      541 gacaaaccaa atgtgtaggg cgtcttgatt caattcaaga caacaaacat ttttaacaag
      601 caagcaatat gctagcaaac aaattgagct taacaatcgc aagattgttc gaacttttta
      661 tgagagtttg atcctggctc aggacgaacg ctggcggcgt gcctaataca tgcaagtcga
      721 acgcttcttt ttccaccgga gcttgctcca ccggaaaaag a
//
LOCUS       DACDIE010000221          741 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.230, whole genome shotgun sequence.
ACCESSION   DACDIE010000221 DACDIE010000000
VERSION     DACDIE010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..741
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.230"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            65..>741
                     /locus_tag="IV250_002948"
     CDS             65..>741
                     /locus_tag="IV250_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8770161.1"
                     /translation="MNRSRQTIHKVYLFFKQGKSALEYYQQYKKNKSNCGRRPLVLPE
                     EQSEYIQRKVVQGWTPDVIVGRAAFPISCSARTIYRMFKKGLFDSSDLPMKGKRKPNG
                     HQERRGKQTFRRSIHEREKDYSQFSNEFGHLEGDTIVGLKHKSAVITLVERLSKVIIT
                     LKPCGRQAIDIEKKLNQWFESVPKNLFKSITFDCGKEFSNWKQISNVNDIAIYFADPG
                     TPSQRGL"
BASE COUNT          255 a          131 c          141 g          214 t
ORIGIN      
        1 tgaacttgta ataatagagt cttatttcaa aatgaatcaa tctgttgcta aaactgccca
       61 ttgcttgaat cgttcaagac aaacgatcca taaagtatat ctgttcttca agcaaggaaa
      121 atcagcctta gaatattatc aacagtataa gaaaaacaaa tcaaactgtg gtagacgtcc
      181 gcttgtttta cccgaggaac aatcagaata tattcaaaga aaggttgttc aaggatggac
      241 acccgatgtg attgttggtc gtgcagcgtt tcctattagt tgttctgctc gtaccattta
      301 tcgtatgttc aaaaaggggc tatttgattc ttctgactta ccgatgaaag gcaagcgtaa
      361 accgaatgga catcaagaaa gacgtggaaa acaaactttc cgccgctcta ttcatgaacg
      421 tgaaaaggat tatagccaat tctcaaatga gtttggtcac cttgaaggtg acactatcgt
      481 aggtctgaaa cataaaagtg ctgtaattac cttagttgaa cgattatcaa aagttatcat
      541 cacattgaaa ccgtgtggta gacaagcgat tgatattgaa aaaaaattaa atcaatggtt
      601 tgaatctgta ccgaaaaacc tattcaaatc catcactttt gattgtggaa aggaattttc
      661 aaattggaaa cagatcagta atgtcaatga tattgccatt tatttcgctg atccaggaac
      721 gccgtctcaa agaggcctaa a
//
LOCUS       DACDIE010000222          736 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.231, whole genome shotgun sequence.
ACCESSION   DACDIE010000222 DACDIE010000000
VERSION     DACDIE010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.231"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..734
                     /locus_tag="IV250_002949"
     CDS             <1..734
                     /locus_tag="IV250_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HAP8770162.1"
                     /translation="DKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRK
                     LMSQMGLTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKI
                     YLSPILDLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQ
                     YQKKLKENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIR
                     WYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT          318 a           94 c          122 g          202 t
ORIGIN      
        1 cagataaata tagcaagata aagcaagaaa ttacagcgat tgtaaaagaa tcaagaaact
       61 cttatggtta tcgtagagtc actttagcgt taaagatgaa aggatataca atcaatcata
      121 aaacagttag aaaattaatg tcccaaatgg gacttacctg tcaaatccga ataaagcgat
      181 ataaatctta taaaggaaca gtaggaaaaa ttgccaagaa tgtgttaaaa cgaaattttt
      241 cagtagatac acccaataaa aaatgggtga cagacgtcac tgaattcaag ataaaaggaa
      301 gaaaaatcta tctatctcct atacttgatt tatttaacgg agaaataatt agttatagta
      361 tatcaactag tccaacatat aaactgattg aagaaatgct tcaacaagca attaaaaaaa
      421 agggaactga gggttcactg attctacatt cagatcaagg atggcagtat cagatgccac
      481 aatatcaaaa aaaattaaaa gagaataaca ttattcaaag catgtctaga aaaggaaatt
      541 gcttagataa ttctgtaata gaaaattttt ttggtgtgct taaatcagag tttttttatc
      601 gagaaaaatt ccgatcaatt gagatatttc aaagtaaatt aaatgagtat attaggtggt
      661 ataataacaa aaggataaaa ctaaagttaa atggactatc tccagtagaa tatcggaaac
      721 agtccattaa atagtt
//
LOCUS       DACDIE010000223          735 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.232, whole genome shotgun sequence.
ACCESSION   DACDIE010000223 DACDIE010000000
VERSION     DACDIE010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..735
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.232"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            40..591
                     /locus_tag="IV250_002950"
                     /pseudo
     CDS             40..591
                     /locus_tag="IV250_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358527.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT          265 a          127 c          130 g          213 t
ORIGIN      
        1 tcatccatcg atcaaaatga acaacgtcaa attgaagaaa tgaaaaagtt tggcgctgaa
       61 aaaattttta tcgaaaaaca atctggagcg acgattagtc atcggccgat tttccaggaa
      121 gctttagatt ttgtcagaga gcaagatatt tttattgtag aagccattga tcgacttgga
      181 cgcaattatg acgagattat cgattcagtg aattatctaa agaagaagaa tgtgcgactt
      241 attatcacca gtttaccgat catggctgaa gcaattggaa atcctctcct agataaattc
      301 atcaaagact taatcattta aatctcagcc atgattgctg aacaagaacg tactgaatcg
      361 aaacgacgcc aggcacaagg aattaaaatc gctaaagcaa acggcgtcta taaaggtcgt
      421 cctaagttat acagtgcaga caccaaagat cctcaacgtc gccttgtcta taggagtatc
      481 gttcaggatc ttgagaatgg ggttgctatc tcaaagattg caacagacta caatgtgact
      541 aggcaaacaa tttaccgaat aaaaaaagaa atcgaccaac taattgtatg atttcttttt
      601 tgaatcacgt aattattaat tagctttgtc atcaatacag acaatcattt tagattcatc
      661 tagctaagtt gattacattt atgttaaaaa gaatttagtt aacataaata taattgatgc
      721 ataaatcgtc cttat
//
LOCUS       DACDIE010000224          723 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.233, whole genome shotgun sequence.
ACCESSION   DACDIE010000224 DACDIE010000000
VERSION     DACDIE010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..723
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.233"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            78..193
                     /gene="rrf"
                     /locus_tag="IV250_002951"
     rRNA            78..193
                     /gene="rrf"
                     /locus_tag="IV250_002951"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            207..279
                     /locus_tag="IV250_002952"
     tRNA            207..279
                     /locus_tag="IV250_002952"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:240..242,aa:Val,seq:tac)
     gene            283..355
                     /locus_tag="IV250_002953"
     tRNA            283..355
                     /locus_tag="IV250_002953"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:316..318,aa:Lys,seq:ttt)
     gene            363..444
                     /locus_tag="IV250_002954"
     tRNA            363..444
                     /locus_tag="IV250_002954"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:397..399,aa:Leu,seq:tag)
     gene            462..534
                     /locus_tag="IV250_002955"
     tRNA            462..534
                     /locus_tag="IV250_002955"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:495..497,aa:Thr,seq:tgt)
     gene            546..617
                     /locus_tag="IV250_002956"
     tRNA            546..617
                     /locus_tag="IV250_002956"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:578..580,aa:Gly,seq:gcc)
     gene            634..719
                     /locus_tag="IV250_002957"
     tRNA            634..719
                     /locus_tag="IV250_002957"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:668..670,aa:Leu,seq:taa)
BASE COUNT          175 a          168 c          199 g          181 t
ORIGIN      
        1 actcggaaaa aaacttatgt gacttcaaat ccagttttga gtgaacaaaa tttactcaat
       61 aaaagataac accacagtgt ggtggcgata gcgagaagga tacacctgta accatgccga
      121 acacagaagt taagcttctt agcgccgatt gtagtgaggg gttgcccctt gtgagagtag
      181 gacgtcgcca cgcttaagtt atccatggag gtttagctca gctgggagag catctgcctt
      241 acaagcagag ggtcagcggt tcgatcccgt taacctccat atgagccgtt agctcagttg
      301 gtagagcatc tgacttttaa tcagagggtc acaggttcga gccctgtacg gctcatattt
      361 ttgcgggtgt ggcggaattg gcagacgcac tagatttagg atctagcgcc ttacggcgtg
      421 ggggttcaag tcccttcacc cgcataattt aagttttaca tgccggctta gctcagttgg
      481 tagagcatct gatttgtaat cagagggtcg agggttcaag tcctttagcc ggcatcttta
      541 aacacgcgga aatagctcag tggtagagca ccaccttgcc aaggtggggg tcgcgggttc
      601 gaaccccgtt ttccgctttg ccaaagcaat cgcgccgggg tggcggaact ggcagacgca
      661 caggacttaa aatcctgcgg tgagtgatca ccgtaccggt tcgattccgg tcctcggcat
      721 ttc
//
LOCUS       DACDIE010000225          709 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.234, whole genome shotgun sequence.
ACCESSION   DACDIE010000225 DACDIE010000000
VERSION     DACDIE010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..709
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.234"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            18..>709
                     /locus_tag="IV250_002958"
     CDS             18..>709
                     /locus_tag="IV250_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8770163.1"
                     /translation="MKELFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSG
                     KSTLLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTN
                     TTSELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLS
                     LNSKLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVK
                     NRTVKTTTIQLL"
BASE COUNT          261 a           87 c          119 g          242 t
ORIGIN      
        1 aagtattttg atttgaggtg aaagaattgt ttgaatttaa caatgtttct ttttcatata
       61 aggatgttaa ggaacaagcg cttgttgacc ttaatttttc aataaaaaaa ggcagtctta
      121 cattgattac tggttcttct gggagtggga aatccacact attaaagtta atgaatggac
      181 ttattccaag actttatgac ggaaaattgg ttggggaaat tttatttgaa aataactcgc
      241 tacaatcctt aacacaagaa agtatatcaa aaaatattgg ctacgtaagt caagatccta
      301 gaggacaatt ctttacaaca aatacaacga gtgagttagt attttcgatg gaaaactttg
      361 gatttacaaa agagcatatg gataaaagaa tcaatgaatt agctgaatta ttagatcttt
      421 caaatatatt agataaaaac attttttcgc tatcaagtgg tgaacggcag aaaatttcta
      481 ttggatgtag tttatcgcta aactctaaat taattatatt ggatgaaccg tcttcaaatt
      541 tggacttcaa agcgactaag aaacttgctg acttactttg tgatttaaaa aataaaggat
      601 ttactattgt tatagctgaa catcgttttt actatttgaa agaattaatt gatcaagttc
      661 tatttgttaa aaatagaact gtaaaaacta caactattca gttgttaaa
//
LOCUS       DACDIE010000226          709 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.235, whole genome shotgun sequence.
ACCESSION   DACDIE010000226 DACDIE010000000
VERSION     DACDIE010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..709
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.235"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
BASE COUNT          287 a           80 c           83 g          259 t
ORIGIN      
        1 ctaggttaag ctatttatta gttcatatat tatattcaga ttaatatgat ccagtattaa
       61 tttgaaggca aaaccaatat atttctacgg attcttgtaa aactatttaa atttttctgc
      121 taataaaata acaaaaatat aatatttagc ttttttatac cattttacga taacattatt
      181 ttattcgtaa tattaaaaca tttaaattgt gaattatttt ttcaaattag atcaattaat
      241 acccttataa aagagcataa taaatggttt ttacatcgtt atttattgat attaatctca
      301 tccaaaaaaa tgaaatcgca tacttttatg agtgattgaa tgtgatttta atacatatac
      361 caattaaaag aaaacttttt agatttttac ttacaaattt ataaaaaaac aagataattg
      421 atactttgat tttgcgaaca ttttatcttt ctgcctaatt cagctttaat atcatgatta
      481 tatcagtaac aaatcgcttt tttagctaat ttaagcatca aataattatt cttaatcaga
      541 aaaaaataac gaataaatgg attttgggaa taggtaaaaa cttgatcggt ttctggctaa
      601 aaataacatg aaaaaggaaa tataaaataa catggatcgt gggatgaata aagaagtttt
      661 atacgcaagg cgcgtagtaa tataaagaaa cttaacacga aacaaaagt
//
LOCUS       DACDIE010000227          708 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.236, whole genome shotgun sequence.
ACCESSION   DACDIE010000227 DACDIE010000000
VERSION     DACDIE010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..708
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.236"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            141..398
                     /locus_tag="IV250_002959"
     CDS             141..398
                     /locus_tag="IV250_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770164.1"
                     /translation="MSNLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
     gene            complement(630..>708)
                     /locus_tag="IV250_002960"
     CDS             complement(630..>708)
                     /locus_tag="IV250_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HAP8770165.1"
                     /translation="NFHLIVTTLQTELNDIISDFNQVNN"
BASE COUNT          290 a           83 c           96 g          239 t
ORIGIN      
        1 ttctgtccac gttcatatta ctccaaagtt ttcctcattc caaaagaaaa gcacagaata
       61 cacaaagaag cgtttttatt tgtataaact atatcaaagt tctatactta aattatcaaa
      121 taaaaaggac gtgaagataa atgtcaaatt tagaaaacaa agaagaaaaa gttgtaaaca
      181 aaattgtttc tgttgtcaat aaactggata aagagttaga cgaacttgat acgttatctg
      241 aaaatccaga aaaaaaacat aacctaaaaa aatggttagt tgaacgaaag gctattcatg
      301 aaattaaaaa agttcttcat gaagctgata aatatgaaaa atatgatgaa aaagaacttg
      361 ataaagaatt taaagaaatc aatgacttat tactataatt gatttatacg actcattttt
      421 gagtcgtcag actgcaaaca acactctaaa tttaggagtt tgtctacagt ctgaataaat
      481 tttaaattta ttgtccataa tgttattatg aatttatagc attaagaaaa gagataagac
      541 tttacatttt tttaggaatg taaagtctta tctcttttct taattgtaca attagtatta
      601 tctgcactac tttctaatta aataaatttt tagttattaa cttgattaaa atcagagata
      661 atatcattta attcagtttg aagggttgta acgattaaat ggaaattt
//
LOCUS       DACDIE010000228          700 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.237, whole genome shotgun sequence.
ACCESSION   DACDIE010000228 DACDIE010000000
VERSION     DACDIE010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..700
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.237"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..351
                     /locus_tag="IV250_002961"
     CDS             <1..351
                     /locus_tag="IV250_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8770166.1"
                     /translation="LGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVYSFARKIGQA
                     LAGGVGGWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFLIVGLILFFLYPLSKD
                     KVLENCELLKQKHK"
     gene            361..>700
                     /locus_tag="IV250_002962"
     CDS             361..>700
                     /locus_tag="IV250_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP8770167.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWS"
BASE COUNT          242 a           84 c          135 g          239 t
ORIGIN      
        1 ttgggtgtgt ttaatactgt tatttgggca aatattactg atgttattga tgatcaagaa
       61 gtaaagtctg atcaaagaga agatgggacc gtatatgcgg tatattcttt tgctagaaaa
      121 ataggtcaag ctctagctgg aggcgtcgga ggttggactc tttctattat aggctatgac
      181 tctttagcaa atgtacaaac gggagatgtt ttagaaaagt tatataacgc ttcaacatta
      241 attccatcaa tttgttttct gattgtagga ttaattttat ttttccttta tccattatct
      301 aaagataaag tcttagaaaa ttgcgagctt ttaaaacaaa aacataaata ggagagaaat
      361 atgaataatt ggatattttg tattgattca gatggctgtg ttatggatac gatgacttat
      421 aaacacaatc tctttttcgg accaattgct gcagagattt ttgatgttca tcatagagaa
      481 aaattcctac aggattggaa taatattaat ttatattcag agactcgtgg aataaacaga
      541 tttttaggtt tagttaaaac attagaaaaa aatgatgtaa catcgattaa tcgattaaag
      601 cactgggtat ctctaacaaa agaattgtct aatccatctt tgaagaggga aatagaatat
      661 tctcagtcag aggatttatt aaaagcttta aaatggtcgg
//
LOCUS       DACDIE010000229          665 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.239, whole genome shotgun sequence.
ACCESSION   DACDIE010000229 DACDIE010000000
VERSION     DACDIE010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.239"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..>665)
                     /locus_tag="IV250_002963"
     CDS             complement(<1..>665)
                     /locus_tag="IV250_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HAP8770168.1"
                     /translation="ETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNY
                     RSGTIAEIDYFYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKD
                     NIRRFLTKKVNYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFD
                     PIEFDNDERLLLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLS
                     KSG"
BASE COUNT          211 a          126 c           73 g          255 t
ORIGIN      
        1 ttcccgattt acttaatttt tctagtacat caaatactct atcactcaag taactcctta
       61 aattgttatc actttcccat tgaactactt ttctataagt tgaattatca gttgaaaaga
      121 tagaattttc atccataaac gcatatatta gcagcaatct ttcatcattg tcaaattcta
      181 ttggatcaaa aatatctaag ttgtcatcaa aatctttatc agttccggta acttttttgt
      241 taattactga aggtgtcttg taagaaatag aatctaaatc aaagcgtttt gattctaaat
      301 tattttgtgc tttctcaatt aaataattca ctttttttgt gagaaacctt ctgatattgt
      361 ctttgctata tgttccataa aaacccttat cttctaaaga gtccctgtac ttcttaactt
      421 ctatatactc ttcagccaaa acttgtggaa ctggttcatc taagaaaaaa acccctacct
      481 cgcctttgct atttttataa aagtaatcaa tttctgctat agttcccgat ctataattat
      541 caactggaga acccaactta tggccaaaaa tcgctaataa tatatcacta ttttctacaa
      601 tttgtttatt aataacactt tgcccagagc tattatttat ataagtagga atcgcattcg
      661 tttcc
//
LOCUS       DACDIE010000230          656 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.240, whole genome shotgun sequence.
ACCESSION   DACDIE010000230 DACDIE010000000
VERSION     DACDIE010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.240"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(138..491)
                     /gene="tnpB"
                     /locus_tag="IV250_002964"
     CDS             complement(138..491)
                     /gene="tnpB"
                     /locus_tag="IV250_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAP8770169.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
BASE COUNT          198 a          143 c          111 g          204 t
ORIGIN      
        1 ttgaagcaac tgaagcaacg tatctgtagt ttccaaaatt tttcaccacc ttgaatcgaa
       61 ctgattttgt tacagaatac cacaagaaac gcacaagaaa taggggttca acacattttt
      121 tgtgttaacc ccccagctta aaaaactcct ttttttcctg gcaaaatagc tcgaggttga
      181 tcgatactta atccttctaa caaccatcga acctgttggt tcgttaaggt tcgaacttcg
      241 tctttcgtcc gaggccattt taacttgccg ttttctatcc gcttataaca gagaagaaaa
      301 ccatcaccgt cccaatagag aagtttgaaa cggtcttgac ggttcccgca aaataaaaaa
      361 acggcatctt cgtaaagctc gagttgatac tcttgttgaa taagactggc aagaccatca
      421 attccttttc gcaaatctgt ttttccgcac acaatataaa tatgtttggt ttgaagtaaa
      481 tcataaagca ttggacaaca gttctttcaa aatagcttga agaatataat tgttaatacc
      541 gttatagatc aaaacttcag aaccattctc tcttttgatg cgcatcgcta acttggcagc
      601 cgcgggttta cctttttctg gtgcactctt ttcgtttcct aattggacag gaacaa
//
LOCUS       DACDIE010000231          646 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.241, whole genome shotgun sequence.
ACCESSION   DACDIE010000231 DACDIE010000000
VERSION     DACDIE010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..646
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.241"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..>646)
                     /locus_tag="IV250_002965"
     CDS             complement(<1..>646)
                     /locus_tag="IV250_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HAP8770170.1"
                     /translation="LFANSKFRKSIREVLPVKIKWMVSILLVLLVYFSFGTNYAKDFL
                     ATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIWKAALKVIRPT
                     LLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLVSSGIIAFICI
                     YAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFNKRDVISI"
BASE COUNT          235 a          116 c           79 g          216 t
ORIGIN      
        1 caattgaaat gacatcgcgc ttattgaaaa gtacatgagc ctccaccatt tcatctataa
       61 aaatagatag tataaaaatt aaaacaatac caagaaacgc ataattcact ttacttctaa
      121 aaaatgtttt gagtaaacta aagatattta acacatagaa agcatagata catataaaag
      181 caattatacc acttgataca agtacttgaa gaaatccatt atgcatgttt ccatgtgctc
      241 tttttagttc attcacatct gtagtatcaa gatctacttt tttcaaaaat tttgcatcgc
      301 tattcccata gaaatcggtc tctccataac caaataacaa agttggtctt ataactttaa
      361 gcgcagcttt ccaaatcgta aaacgaccag ctgaaatttc ggagttatca ctagaatgct
      421 gtaatattac ttctttttct ggaagtggag ttttatctga ttcatttgac tctataactt
      481 ctacttttct atcaatcgat acaggaattg ttgctaaaaa atccttagca taattagtac
      541 caaaagaaaa atataccaat agaactaata atatgcttac catccatttg atttttacag
      601 gtagtacttc tctaatgctc tttctaaatt ttgaattggc aaacaa
//
LOCUS       DACDIE010000232          645 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.242, whole genome shotgun sequence.
ACCESSION   DACDIE010000232 DACDIE010000000
VERSION     DACDIE010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..645
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.242"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(85..>645)
                     /locus_tag="IV250_002966"
     CDS             complement(85..>645)
                     /locus_tag="IV250_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP8770171.1"
                     /translation="GLLFAPSIISIFSLTITGFIAFTLVFGKKDTKKMFSKPVAPVKN
                     IAKYFFINVVISAAVSVFLQQILKWGLTGNPINEAFSPLLFIILPIMILGEELFSVYF
                     LAIFSSKFSIPVASFLSAIIFGFIHYSTYDNGNILHTVAHILLIQGVARLLFNQAAIK
                     SNSIITSWAVHVIFDFTAILLVVLFS"
BASE COUNT          239 a          103 c          111 g          192 t
ORIGIN      
        1 atccattgat acatctgtct ttttgtttaa aggaaataaa gtatgctttc ttagcaatag
       61 agcatgtatt atttctataa aaatttagga gaataaaacg actagcaata tagctgtgaa
      121 atcaaatatc acatgaactg cccaacttgt aatgatcgag ttgcttttaa tggcagcttg
      181 gttgaataac aatcttgcca ccccttgtat caaaaggatg tgtgctacag tgtgtaaaat
      241 atttccatta tcataagtac tgtaatggat aaatccaaag atgattgctg acaagaaact
      301 agcgacagga atgctgaact tactagaaaa aatagcgaga aaatacactg agaacaattc
      361 ttctccaagt atcatgatag gcaaaataat aaataataac gggctgaaag cctcatttat
      421 tggattgcct gttaaacccc atttcaaaat ttgttgcaaa aataccgaga ctgcggcact
      481 gattactaca ttgataaaaa aatatttggc tatatttttg acaggagcga caggtttcga
      541 gaacattttt ttcgtatctt ttttaccaaa aacaagtgta aatgctataa atccagtgat
      601 agttaaagaa aaaatgctaa taatggatgg cgcaaatagc aaccc
//
LOCUS       DACDIE010000233          642 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.243, whole genome shotgun sequence.
ACCESSION   DACDIE010000233 DACDIE010000000
VERSION     DACDIE010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..642
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.243"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
BASE COUNT          232 a          111 c           77 g          222 t
ORIGIN      
        1 aattcgcttg tggcgaaacc cgacctggag tttgtctctg tattttgaac cctaaaaatt
       61 aatcaaacag actgatattc tcataatcag cgcgaaataa gccattaagt acagttttcc
      121 tacctatttt tgataacccg ctggttagtt aatattcctt ttccgagaga gataaatgat
      181 tggttaaatc tatatacatc tcaaccttta aaaatgtttt cgaaatactt catcagtaca
      241 tgtcatacaa attaccagtg cattctcaaa ttctagttct ttaattgatt tcagttgagg
      301 agcagttact tttggcggaa atttccattt ttgtccaaac ctcttccccc gatgaaattt
      361 ctttgttgca attattgcaa tgaaaaattt ttgtttcaaa aatatacata attacttcct
      421 ccaataaaaa ctatctacct ctcaaattat ttttctctaa taagaaaaac agaaaaggta
      481 ataagataca acagtgatga ttcctaataa agaaaagact acaataatat ttttctaact
      541 tatcgccttc aaagaaatag tttcttattt ttcactaaaa taaaaaattt attgcaaaaa
      601 aaatagaagt gacaaaatta tcaatcattt caaggtaata ta
//
LOCUS       DACDIE010000234          630 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.244, whole genome shotgun sequence.
ACCESSION   DACDIE010000234 DACDIE010000000
VERSION     DACDIE010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..630
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.244"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            133..387
                     /locus_tag="IV250_002967"
     CDS             133..387
                     /locus_tag="IV250_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP8770172.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            388..>630
                     /locus_tag="IV250_002968"
     CDS             388..>630
                     /locus_tag="IV250_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP8770173.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFY"
BASE COUNT          229 a          100 c          129 g          172 t
ORIGIN      
        1 gttttcagcg ttctaagtgg cttattttgt ccaataataa tatggagtgg aatcccttaa
       61 gaaacaccgt tggcaattgt tgttataaca tgttataatt tattcatcaa aaactaaaag
      121 gagtgtgata acatgggaaa agaactaaag gttagaaaga tcggcaatag cgtgggagtg
      181 atccttccaa gctctcttgg attaaaaagc ggagacatta tccaagccaa acaagaaggc
      241 aacttattca tattagatac tactcaaata gcaaaagaac acgatcgcaa attgattgaa
      301 gaaagtttcc aagactttga gaaaggttta acagtttcag aaattgaaat ggtaaaagct
      361 ttcggaaaat acggctggag tgaatagatg aaagagtaca cgctagttta ttctaaaagt
      421 tttcagacca gccttgaaac gataatagaa gaatgggaaa atgagcttgc tctttctcca
      481 gccagcataa aaagatttgt aggtgcgatc cagaaggctt tagaactaac caaaaatttt
      541 ccaactatgc atgaggaagt ttccgaaatc tatggaatgg atatcccaac atatcggatc
      601 gtgatcggca aaagctatgc cttattttat
//
LOCUS       DACDIE010000235          628 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.245, whole genome shotgun sequence.
ACCESSION   DACDIE010000235 DACDIE010000000
VERSION     DACDIE010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..628
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.245"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..447)
                     /locus_tag="IV250_002969"
     CDS             complement(<1..447)
                     /locus_tag="IV250_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP8770174.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKL"
BASE COUNT          185 a          115 c           86 g          242 t
ORIGIN      
        1 caactttctc ttttcaaaaa aaatttgata tttttttaga tttcttttta gtctttgtaa
       61 tactttataa gccacagaat aactcacgtg atgctgttta gcaaaatttt ctactgagat
      121 atatttttcc tgaaagaaag ccaataccat tttcatagat aaagaatccg ttatataacg
      181 agataacaga gctttgctag aacattttcc attaatttcc agacatatat ccaattcact
      241 gacggttact cgcaaatttt tctctagatc gaacttttgt aaatcatgtt tcagcttatc
      301 gatcgttttt ttcaagagaa atgtcgatag cgacaactct tcttgaacat tcgatattga
      361 ctctgataaa tttgagcttg cttctaaata tttaagtaat gaataacttc tgtgatcttc
      421 tttgcttaaa aaaaactcat aattcattag tcatcagctc tttctacgta tattactttc
      481 catgcattca ccaactagct ggggataaaa ctgatttcct ggatccgagt cactgttgtc
      541 agcgattctt gtttgtataa tatacatcgt cctgtctttt ttttaacatt ttggagagcg
      601 ttttatttgg cctttctcgc gatcaaac
//
LOCUS       DACDIE010000236          612 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.246, whole genome shotgun sequence.
ACCESSION   DACDIE010000236 DACDIE010000000
VERSION     DACDIE010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..612
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.246"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>612
                     /locus_tag="IV250_002970"
     CDS             <1..>612
                     /locus_tag="IV250_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HAP8770175.1"
                     /translation="KYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQT
                     LPAYKRTNDTLETTIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNA
                     FKSRALNDKYVAIFMDATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTY
                     VWNELLQDINSRGVQEVLLFITDGLKGMKDTIHQIYPKAKYQH"
BASE COUNT          221 a          113 c          107 g          171 t
ORIGIN      
        1 aaaatatgat cgcactggtt ttaattcagg taattcgaga aacggttctt actttcgatc
       61 aatcaaaacc gaatatggtg aattaacatt ggaaatacct agagatcgta atggtgagtt
      121 taaacaacaa actttaccag cctacaaaag aacaaacgat acattggaaa ccactattat
      181 ccatttattc gaaaaaggtg ttacgatgtc tgaaattgct gatttgatcg aaaaaatgta
      241 cggtcatcac tatactccac aaaccatgtc caacatgact aaagttctga ctgaagaagt
      301 aaatgccttt aaatccagag ccttaaatga taagtatgtc gctattttta tggacgctac
      361 ttacattcca ctaaaacgtc aaaccgtatc caaagaagcg atttatattg ccattggtat
      421 acgagaagac ggcactaaag aagtactgag ttatgcgatt gctccaactg aatcaacata
      481 cgtttggaat gagctgctac aggatattaa ctccagagga gttcaagaag tcttgctttt
      541 tattacggac ggcttaaaag gcatgaaaga tactatccat caaatttatc ctaaagcaaa
      601 atatcagcat tg
//
LOCUS       DACDIE010000237          595 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.248, whole genome shotgun sequence.
ACCESSION   DACDIE010000237 DACDIE010000000
VERSION     DACDIE010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..595
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.248"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..581
                     /locus_tag="IV250_002971"
     CDS             <1..581
                     /locus_tag="IV250_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8770176.1"
                     /translation="LAPTESAYVWKEILQDLKDRGLKEVLLVVTDGLSGINDSIHSVY
                     PNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLVYQASSKEEANNQIRFMIDKWKKQ
                     YPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEGFNKQLKKYTKRKEQFPNEESLER
                     FLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
BASE COUNT          221 a           88 c           97 g          189 t
ORIGIN      
        1 gtcttgctcc aactgaatct gcttatgttt ggaaagagat tcttcaagac ctaaaagacc
       61 gtggtttaaa agaggtttta ttagtcgtaa ctgatggttt aagtggcatt aacgatagta
      121 ttcatagtgt ttatccaaat gctcaatttc aacaatgttg tgtccatata tctagaaata
      181 tcgctcataa ggttcgtgtt agtgatcgac aagaaatctg tagcgatttc aaattagttt
      241 atcaagcaag ttcaaaagaa gaagcaaata atcaaataag gtttatgata gataaatgga
      301 aaaagcagta tccacgagtg gttaaattgc tcatgaatcc tgctatatta accttttata
      361 atttccctcc gtcaatcaga agaactattt attcaactaa tttgattgaa ggttttaaca
      421 aacaattaaa gaaatatacc aaaagaaaag agcaatttcc taatgaagaa tctctggaga
      481 gatttctagt atctcaattc aacaactata atcaaaagtt tctatgccga gttcataaag
      541 gttttaaaga aatacatgat acattagaat caatgtttta acttgatcaa cagaa
//
LOCUS       DACDIE010000238          593 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.249, whole genome shotgun sequence.
ACCESSION   DACDIE010000238 DACDIE010000000
VERSION     DACDIE010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.249"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(318..581)
                     /locus_tag="IV250_002972"
     CDS             complement(318..581)
                     /locus_tag="IV250_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circular bacteriocin, circularin A/uberolysin
                     family"
                     /protein_id="HAP8770177.1"
                     /translation="MKKNLLLVLPILGFAGFFVGVPMLSANIGISSYAAKKVIDIINT
                     GSTVATIISIVAAVVGGGLITAGIVATAKSLIKKYGAKYAAAW"
BASE COUNT          224 a          104 c           79 g          186 t
ORIGIN      
        1 gccttccttt tttgtatgga ttttttcatt tacacaaaat attttatact ctcctgaatt
       61 ttttcatgtt gaaaacatca ctaatcaaag gcaaattata atttagaaat agaatgaaaa
      121 cactaaatga aaatagaaca ttagaaaaaa tagaatcaaa aaaacccaag actagtgccg
      181 aaataaggga aacaatcgaa gctacaaaaa ataatgattt cgttgaacag gaccaattta
      241 tttgtttccg atatttctca aaaagaactt tctttgggaa agttaaaggc gactgtaaag
      301 tcgcctttag tcctttttta ccaagctgct gcatatttag ctccatattt tttaattaat
      361 gatttggctg tagcaacaat accagctgta attaatccgc ctccaacaac tgcagcaaca
      421 atagaaatga ttgtagcaac cgtgctacca gtattaatta tgtcaataac ttttttagct
      481 gcataagaag aaattccaat gttagcactt agcattggta caccaacaaa gaatcccgca
      541 aaacctaaaa ttggtaaaac taataataaa ttctttttca taataattcc tct
//
LOCUS       DACDIE010000239          577 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.250, whole genome shotgun sequence.
ACCESSION   DACDIE010000239 DACDIE010000000
VERSION     DACDIE010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.250"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..473)
                     /locus_tag="IV250_002973"
     CDS             complement(<1..473)
                     /locus_tag="IV250_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8770178.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGA"
BASE COUNT          195 a          118 c           74 g          190 t
ORIGIN      
        1 gctccaacat ttcggaatgt cgatagcatt gttctttctt ctgtaataga agtaatagct
       61 gatgccattg atccataagg aatatttata gatgtgtaaa aaatactccc ccataatata
      121 tacgtaacat acatgtatac taccttaacg ctcatagaag ctgaaatcat agaagtttga
      181 tacattaaaa aacttgctaa agcgactgga ccactcattc ttaaaatcca cggtttaaac
      241 ttaccctgct tagttgcttt cgatctatca acaatacgcc ccatagttac atcagtaact
      301 gcatcgataa atcttgctac taaaaataat gttccaacgg cagctccgct tatccccaat
      361 acttttgtat aaaagatcat taaaaaagaa ctagcaaaaa tgaaagtaaa atcgtttcca
      421 aaatctccaa acatatatcc tattttgtct cttataccaa attttctttc catacaattc
      481 tctcccttct gttgttaagc gttttcattc tgatgaaagt acgtactata ctctgaagcg
      541 taaagagtca gtatttattt tttacatttc agagtat
//
LOCUS       DACDIE010000240          573 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.251, whole genome shotgun sequence.
ACCESSION   DACDIE010000240 DACDIE010000000
VERSION     DACDIE010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.251"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            1..573
                     /locus_tag="IV250_002974"
                     /pseudo
     CDS             1..573
                     /locus_tag="IV250_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
BASE COUNT          227 a           98 c          108 g          140 t
ORIGIN      
        1 agaaaaagat aatcttacgc ctgaacaaaa agaattgtta gaactacgta aaagaaacca
       61 gcaattagaa atggaaaatg atattttaaa gcaagcagcg ctgatattcg gacgaagaga
      121 caagtaatcg atgcgaataa gcatctttac cctatatcag cgatgtgcag aatattaggt
      181 ctatcacgtc agtcctatta ttatcaatca aaaccaaaga aagacgaatc agaacttgaa
      241 gaagtagtcg ctgaagaatt tatccgcagc cgaaaggcct acggctcaag aaaaataaaa
      301 aaagccttat caaaacgagg cattcagatc agccgacgaa aaattagtag aatcatgaaa
      361 aatagaggat taaaatcgag ctatactgtt gcttatttta aagtacatca ttctacttgc
      421 aatgaagcca aaacgacaaa cgtattgaat cgtaaattct taagagacaa cccattagaa
      481 gcgatcgtaa cagacttgac ttatgtacga gtcgggaaaa aatggaatta tgtctgtttc
      541 attttggatc tgttcaatcg agaaattctc ggc
//
LOCUS       DACDIE010000241          573 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.252, whole genome shotgun sequence.
ACCESSION   DACDIE010000241 DACDIE010000000
VERSION     DACDIE010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.252"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>573
                     /locus_tag="IV250_002975"
     CDS             <1..>573
                     /locus_tag="IV250_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP8770179.1"
                     /translation="ILARLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESLCTV
                     FDLFIPEKEKILYSLAFARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQF
                     YMDFREQNQHLLPVILEKENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLI
                     VKIEQIFSNESIKIQNDFMENCNIVISDCPL"
BASE COUNT          202 a           99 c           95 g          177 t
ORIGIN      
        1 attttagctc gactttctat cacgaaaatc gaatcggaag aaacaagctt ggagcaatac
       61 aaacttttaa aaaaagcaaa cgttcctcat gttctttatt caagagaaat ggtggagtcc
      121 ctttgtactg tatttgattt gtttatacct gaaaaagaaa agattcttta ctcattggca
      181 ttcgcacgaa ataaacttta taatggattt cttaacttca atcaaccaaa tacgcccctt
      241 cctactttca tgctttatca agaacatagc gtattgaaag aagtccaaac cgccattgaa
      301 caattttata tggatttcag agaacaaaat caacacttgt taccagttat tttagaaaag
      361 gaaaatgtgc aatggatggt tgaagaccta gtacatttgt atgatcgcta caaaaaacag
      421 ccgcgaattg tgattggtgt gaactatacg cgtgattttt atatcagtaa agatctgatc
      481 gttaagatag aacaaatttt ttcaaatgaa tcgatcaaaa tacaaaatga ttttatggag
      541 aactgcaaca tcgttatcag cgactgccct ttg
//
LOCUS       DACDIE010000242          567 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.253, whole genome shotgun sequence.
ACCESSION   DACDIE010000242 DACDIE010000000
VERSION     DACDIE010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..567
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.253"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            73..>567
                     /locus_tag="IV250_002976"
     CDS             73..>567
                     /locus_tag="IV250_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770180.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTI"
BASE COUNT          209 a           80 c          117 g          161 t
ORIGIN      
        1 taacagccta agatatttgg agttcagaga gggcgcgttt gattttcaaa cttcgcaaca
       61 gaaccgagaa ccttgggttt tcaagtgctt tttggttcat taatcttaac catagtgttt
      121 ttaaagtgga aaacaagcaa caaaaaaagt atgtggagcc ttgtaggaat agttcttatt
      181 atttttactg tgactgcagc aacagaagca aattattcgc ataaaattca gtcgtataaa
      241 ggtcgtttag ttatgaccaa aaaagatgtt acacgttata catcctcaag aactggaggc
      301 ctatatgact atgcaccatt agatgcattg aaatataaag atcatttaaa gaaacatgaa
      361 gtaaaagttg gtgatcggtg ggagaatgca tctcataaag catataattc caaaatagtc
      421 tatacagtag aagatgccga tgagcaaaat atcacattac ctgtattcaa ttattgggga
      481 acagttgtga aagtaaatgg gaaaaagagc actatagaaa atgacaatgg tttagtgtct
      541 attaaaggta cggggaaaag aactata
//
LOCUS       DACDIE010000243          560 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.254, whole genome shotgun sequence.
ACCESSION   DACDIE010000243 DACDIE010000000
VERSION     DACDIE010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..560
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.254"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..>560)
                     /locus_tag="IV250_002977"
     CDS             complement(<1..>560)
                     /locus_tag="IV250_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HAP8770181.1"
                     /translation="MYGLPVLLSTGMADIHEVSEAVDCFIDSPVTILHCTSNYPASSE
                     DINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKVIEKHFTLDKELPGPDHKA
                     SLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSRKSLVVTENLNVGDILTEK
                     SLTIKRPGNGIAPKFLYQYIGKKVKR"
BASE COUNT          185 a          119 c           78 g          178 t
ORIGIN      
        1 gtcttttaac ttttttgcca atatattgat ataaaaactt aggagctatt ccatttccag
       61 gacgttttat tgtaagagat ttctctgtaa gaatatctcc cacatttaaa ttctcagtga
      121 ccactaggct ttttcttgat acttcttttg tactaagttc agaaggcatt gctcgcttaa
      181 caccatcacc taaaaatatt tcagtgtttc taatatgctt tacaaaatct gcaaattcct
      241 gaggatctaa agaagcttta tgatctggac caggtagttc cttatccaag gtaaaatgtt
      301 tctcaattac cttagcaccc atcgcaacag cacaaatacc aacatcatat cccaatgaat
      361 gatcggagta cccaacgggt atcccgaaca tttcttttaa tgtgttaatc gccaataaat
      421 taatatcttc agaactagcc ggataatttg aagtacaatg taaaatagta actggactat
      481 ctatgaaaca atctacagcc tcactgacct catgaatatc agccatacct gtagataata
      541 aaactggcaa cccatacata
//
LOCUS       DACDIE010000244          558 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.255, whole genome shotgun sequence.
ACCESSION   DACDIE010000244 DACDIE010000000
VERSION     DACDIE010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..558
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.255"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(76..378)
                     /locus_tag="IV250_002978"
     CDS             complement(76..378)
                     /locus_tag="IV250_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770182.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
BASE COUNT          194 a           97 c           75 g          192 t
ORIGIN      
        1 ctaacttaaa aaatatccct tttataagta tggtgataaa atccgttccg ggctaccttt
       61 tttaggtagc ctactttaat ctttatacct ttctggatca acgaaagtat acttcaaata
      121 gtcataacgc cgatgatcgc tccgtgcgtc tggcacatca gtcacgatat caaacaaaaa
      181 atacacatct ttcttcattc tggtttttgt tgccggaatt ttaaagtagt tcttattaga
      241 gtaatagaga ttgattaata agctatcttc gattgctaaa aagaaaactt ctgaatccca
      301 caccttataa aaatctttga caaatctatt cgaaggatca aatttaaacc ataattgtgt
      361 ttttccttcc atcaacataa tgttcacctc aaaaagagta tacgaactaa tgttcttttt
      421 gtaaagtagg aatatcattg ataatatgga tggaacatat atattaaaaa gtgttctttt
      481 tgaatgctat tttgaatgcc attttcattc atttaacgaa aatctgcgaa aataaaaaat
      541 gctcaaaccc ttataaaa
//
LOCUS       DACDIE010000245          546 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.256, whole genome shotgun sequence.
ACCESSION   DACDIE010000245 DACDIE010000000
VERSION     DACDIE010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.256"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            236..>546
                     /locus_tag="IV250_002979"
     CDS             236..>546
                     /locus_tag="IV250_002979"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734812.1"
                     /note="malic enzyme; oxaloacetate-decarboxylating;
                     NAD-dependent; catalyzes the formation of pyruvate form
                     malate; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAP8770183.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     N"
BASE COUNT          182 a          101 c           77 g          186 t
ORIGIN      
        1 tctatttatc tatctaacat atcctttatt cttctacttt tctttcttta gcatataaga
       61 gatacgcata gtaatcaagc aaggattgtt gatataaaaa gaacaaatac cgctattttg
      121 atttcttttg actctattac tcatataaat aaaatttatt atacctgctc tttttgacta
      181 tatcctcatc tgactcctat acttcgtttg tattaaaact taggaggaat cattcatgct
      241 tacagggcta aacttattaa ataatccttt tctaaataaa ggaacagctt ttacaaaaga
      301 agaacgggca aaatacggaa tcactggtat gcttccaagt actgtccaaa cattggaaca
      361 acaatctgtc caagcttacg gtcaatactt aagcaaacag accgatttgg aaaaacgtat
      421 cttcttaatg aatctattca atacgaaccg gacattgttt tataaattga tgggcgaaca
      481 tttagttgaa ttcatgcctg tcgtttatga tcctgtcgtt gcggattcaa tcgaacaata
      541 caacga
//
LOCUS       DACDIE010000246          538 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.258, whole genome shotgun sequence.
ACCESSION   DACDIE010000246 DACDIE010000000
VERSION     DACDIE010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..538
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.258"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            54..467
                     /locus_tag="IV250_002980"
     CDS             54..467
                     /locus_tag="IV250_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HAP8770184.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
BASE COUNT          202 a           83 c           98 g          155 t
ORIGIN      
        1 ttattacgta aatagggata aataaaaaat acttattgaa aggatgatca aaaatggcaa
       61 atgtacctag cattcgcgac atgttccctg atttcaatga cgtgtttagt ccagcattca
      121 atgatttttt aggtgtttca agttatccaa aagtagattt agtcgaaaat gaaaaagaat
      181 acaaactaac tgctgatatg cctggctgtg acaaagaaga cacgacagtt gaatattcag
      241 ataatacttt gacgatctcc gccaatcatg agtcccatac agaagataaa gaagatggca
      301 actatgtacg gaaagaaaga cattcggtct cttacaaacg ttcattctat cttcctaatg
      361 ttgatgaaga aaaaatcact ggaaccttta aaaacggtgt gctgaaattg gtattaccta
      421 aaacagctta tcagccaaaa gaaacaaaaa aaattgagtt aaattagaac ttatcgcctc
      481 tcgatgaatg aggggcgatt tttatatatt tggataagaa cgagtatgtt taaatttt
//
LOCUS       DACDIE010000247          524 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.260, whole genome shotgun sequence.
ACCESSION   DACDIE010000247 DACDIE010000000
VERSION     DACDIE010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.260"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>524
                     /locus_tag="IV250_002981"
     CDS             <1..>524
                     /locus_tag="IV250_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAP8770185.1"
                     /translation="TMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVALFD
                     TAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMRQQL
                     TTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYLSQI
                     VENICYRNAYNYFN"
BASE COUNT          167 a           77 c          117 g          163 t
ORIGIN      
        1 acgatgcaaa tgcatttaaa tgctttaaga aataattcaa gtatattaaa ggaacaaatt
       61 ggagtagatg tcggtggtga ttcgatgggg gatcaagcta gtttgacaca aaatgttgta
      121 gcattgttcg ataccgctga aaagagtggg tgcttaccaa aaacaatttt atattcatta
      181 aatccaactg attggcttcc actagcaaca gcaatgcaaa gcttccaagg gggtatgatt
      241 caaaaaatcc aattgggatg tgcttggtgg ttcaatgata cagctgaagg aatgcgtcaa
      301 cagttgacta cgatggcaca acaaagtttg ttgtcgaatt ttacaggtat gttgactgat
      361 tctcgaagtt ttctaagcta tcctcgccat gagtattttc gtcgagtatt atgtcaatta
      421 ctaagcgagt gggttaaagc aggccgaatt ccagatgatg aaatgtatct tagtcagata
      481 gtggagaata tttgttatcg taatgcgtat aattatttta attt
//
LOCUS       DACDIE010000248          524 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.261, whole genome shotgun sequence.
ACCESSION   DACDIE010000248 DACDIE010000000
VERSION     DACDIE010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.261"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            245..463
                     /locus_tag="IV250_002982"
                     /pseudo
     CDS             245..463
                     /locus_tag="IV250_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
BASE COUNT          185 a           72 c           82 g          185 t
ORIGIN      
        1 cataactgaa atacctaaat gacatataca gatatgataa tttatgtatt gatttttcta
       61 acaaaagcac atattttaat atatactacc tattttaggt agtatatatt tttagataca
      121 atatcgatca gttttgagga aatgaaattc tagatatttt ttgctacata gagttacttt
      181 caatattttt taacgtaatt aggcagaagt cactcatcct ctataggttt gtgatgaaat
      241 tgccattgat ccatttaagt aagagcaaaa aactggaggt ttatcaggta attggtctaa
      301 tggatttaag tatgcggtca ccgtgtatag agttgcttat gagattcaag aaaataaagt
      361 tattccagta ttgctatatg gaacacataa caatttttat gaattagccg agaacactcg
      421 caactttatt cgtgtagatg aatcggcttc ggttgagtta taactttagc tatatcaatc
      481 aaccgaccga catatgttaa atctatatat aataaaaata ccta
//
LOCUS       DACDIE010000249          520 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.262, whole genome shotgun sequence.
ACCESSION   DACDIE010000249 DACDIE010000000
VERSION     DACDIE010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.262"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(56..298)
                     /locus_tag="IV250_002983"
     CDS             complement(56..298)
                     /locus_tag="IV250_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770186.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            419..491
                     /locus_tag="IV250_002984"
     tRNA            419..491
                     /locus_tag="IV250_002984"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:452..454,aa:Thr,seq:cgt)
BASE COUNT          161 a          102 c           84 g          173 t
ORIGIN      
        1 acagctttcg tttgcatata aaaaaaccat cccaaaaatg cagagatggt caaattcaaa
       61 tcttttttag ttctttgtag ttaacgatta ctacaccctt actttgcgtc atcaagttcg
      121 cattttctgc agtttcatca atctcaacaa cagcggaatt tttcaattgc tttgaaatcg
      181 ttccagtttg cgacttgccg tgaattgtga acattacgtg agtattgatc ttgaattttt
      241 caccaatttc cgatggtacg atagttgtat caaattttcc aaaagttgcc attttcataa
      301 aacctgcctt tcacatcaat tataccacgt tttatgaaaa atatcatgaa attaaactaa
      361 ttttccatcg gactctttac aatcagccaa atagagtata taatttcttt tggataaggc
      421 cagcttagct cagttggtag agcaacgcac tcgtaacgcg taggtcgtag gttcgatccc
      481 tatagctggc attaaaagag aagacagata tatcaaggat
//
LOCUS       DACDIE010000250          513 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.264, whole genome shotgun sequence.
ACCESSION   DACDIE010000250 DACDIE010000000
VERSION     DACDIE010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.264"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..132)
                     /locus_tag="IV250_002985"
     CDS             complement(<1..132)
                     /locus_tag="IV250_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP8770187.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          185 a           94 c           92 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag aca
//
LOCUS       DACDIE010000251          506 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.265, whole genome shotgun sequence.
ACCESSION   DACDIE010000251 DACDIE010000000
VERSION     DACDIE010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.265"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            21..>506
                     /locus_tag="IV250_002986"
     CDS             21..>506
                     /locus_tag="IV250_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HAP8770188.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIK
                     YL"
BASE COUNT          177 a           82 c          104 g          143 t
ORIGIN      
        1 agatagcagg aggaatgacg atgaatcatt ttaaaggaaa gcaatttcag caggatgtga
       61 ttattgtagc cgtgggctac tatcttcgtt ataaccttag ctatcgtgaa gttcaagaaa
      121 tcttatatga tcgtggcatt aacgtttctc atacgacgat ttatcgttgg gtgcaagaat
      181 atggcaaact actctatcaa atttggaaaa agaaaaataa aaaatccttt tattcatgga
      241 aaatggatga aacgtacatc aaaattaaag gaaaatggca ttatttgtat cgagccatcg
      301 atgcagatgg tttaaccttg gatatttggt tacgtaaaaa acgggacaca caagcagcct
      361 atgcttttct taagcggtta gtgaagcagt ttgatgaacc gaaggttgta gtcacagata
      421 aagccccctc tattacaagt gcctttaaga aactaaaaga atacggcttt tatcaaggga
      481 cagaacatcg taccattaaa tacctg
//
LOCUS       DACDIE010000252          488 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.266, whole genome shotgun sequence.
ACCESSION   DACDIE010000252 DACDIE010000000
VERSION     DACDIE010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.266"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..414)
                     /locus_tag="IV250_002987"
     CDS             complement(<1..414)
                     /locus_tag="IV250_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770189.1"
                     /translation="MGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEINRKIYWVS
                     IPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELIFSLTTPGN
                     ALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFF"
BASE COUNT          185 a           78 c           62 g          163 t
ORIGIN      
        1 aaaaaacggc tttccaaatg atgaaatacc taaatctaac ttattaagaa agttgaaatt
       61 tttatattca ggaaaccatt tattaatttc atgtgcagca cgtaatgcat tccctggagt
      121 tgtcaaagag aaaattaatt ctgctaagct tattattgaa aatactgata acttataatt
      181 atatcgtcca ttgacaatca aatataaact aactatactt gttagaacaa aaaaacaggc
      241 gttgacttgt tcttgattag ttaaaaatat caataaagga atacttaccc aatatatttt
      301 tctatttatt tcttctcctc ttaagagttg aaaaaacgga taaaaagtta gtaaactaaa
      361 tgctactggc cactgatagt tcaaggtggt tgctctccaa ccggtttccc ccattactgt
      421 caatggtatt aaacagtata tagagcaaac caataataaa tttttaacat ttattttctc
      481 aaaaacat
//
LOCUS       DACDIE010000253          486 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.267, whole genome shotgun sequence.
ACCESSION   DACDIE010000253 DACDIE010000000
VERSION     DACDIE010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.267"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..426
                     /locus_tag="IV250_002988"
     CDS             <1..426
                     /locus_tag="IV250_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAP8770190.1"
                     /translation="LKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIP
                     ELLSVMDVFVMPSLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQ
                     EWAKKVIKEPYANKITQELKSYDVSYTTEQMESIYSRDH"
BASE COUNT          192 a           53 c           93 g          148 t
ORIGIN      
        1 ctaaaaatga attctgattc ttacttgatt ttagtaggtg aagggtcact tagaaataaa
       61 ctgaaagcca aagcagtaga attggatata gagaaaagag taaaattttt aggagaaaga
      121 aacgatattc ccgagttatt atctgtgatg gatgtttttg taatgccttc tctttatgaa
      181 ggtctgccaa tggttggatt agaagcacaa gcagcaaaat taaaattagt cttagcagat
      241 actatttcca aagatacaaa gttaacaaat aatgtggatt ttgtatcatt agatgaaaca
      301 ccacaagaat gggcaaaaaa agtaataaaa gagccgtatg ctaataaaat aacacaagaa
      361 ctaaagagtt atgatgtttc gtacactact gaacaaatgg agagtatata tagtagagat
      421 cattaaaaat gttcaagata ttttaggagt gattattttt gaaaatatgt tatttgatat
      481 tggcat
//
LOCUS       DACDIE010000254          484 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.268, whole genome shotgun sequence.
ACCESSION   DACDIE010000254 DACDIE010000000
VERSION     DACDIE010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.268"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>484
                     /locus_tag="IV250_002989"
     CDS             <1..>484
                     /locus_tag="IV250_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP8770191.1"
                     /translation="KVLYYLWKKKNRQSFYSWKMDETYIKIKGRWHYLYRAIDADGLT
                     LDIWLRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTKTEHR
                     TVKYLNNLIEQDHRPIKRRNKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFGFSVS
                     T"
BASE COUNT          164 a           96 c           96 g          128 t
ORIGIN      
        1 caaagtcctc tattatcttt ggaagaagaa aaatagacaa tccttctatt catggaaaat
       61 ggacgaaacc tatatcaaaa ttaagggacg ttggcattat ctttatcgtg caattgatgc
      121 ggacggctta accttagata tctggttacg aaagaaacgg gatacgcaag cagcctatgc
      181 tttcttaaaa cgactccata aacagtttgg tgagccgaaa gcaattgtga ccgataaagc
      241 accttctctt ggctccgcct ttagaaagtt acagagtgtg ggtttatata ctaagacaga
      301 gcaccgaact gtgaagtatc ttaacaattt aatagaacaa gaccatcgac ctattaaacg
      361 acgaaataaa ttttatcaaa gtctccgtac agcctcttcc acgattaagg gcatggagac
      421 cattcgagga atatataaaa agaaccgaag aaatggaacg ctcttcggct tttcggtgtc
      481 tact
//
LOCUS       DACDIE010000255          467 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.270, whole genome shotgun sequence.
ACCESSION   DACDIE010000255 DACDIE010000000
VERSION     DACDIE010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.270"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            204..458
                     /locus_tag="IV250_002990"
     CDS             204..458
                     /locus_tag="IV250_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8770192.1"
                     /translation="MQESTVPSLPLENSDKALLFLIAHRSELQSDDIVISFYQKIDRD
                     YLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTPF"
BASE COUNT          147 a           94 c           78 g          148 t
ORIGIN      
        1 tgggaactgt aatacttttt tctgcctgtg gatataaaac aaagaattct gttaaagaat
       61 cttcagcagc catttcaaca actgaggttg gttcaactaa aagtacttct actacttcca
      121 ttactaatac ttctactggt aatgtagatt cgcaacatag ccaatcaagt attcagtcgt
      181 ttagcatttc ttcaacatct gaaatgcaag aaagcactgt accttctctt ccattagaaa
      241 actctgacaa agcacttcta tttcttattg cccaccgttc cgaattacaa agtgacgata
      301 ttgttattag cttttatcaa aaaattgatc gagattattt gttcacagct tccagcaaac
      361 aaatccgttc ccaaggagga tctggaagcg ttggttttta tcgagtgtca cccgaaggat
      421 cgattacgat gacagatgca aatggaacac cattttgatt agatgat
//
LOCUS       DACDIE010000256          466 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.271, whole genome shotgun sequence.
ACCESSION   DACDIE010000256 DACDIE010000000
VERSION     DACDIE010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..466
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.271"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>466
                     /locus_tag="IV250_002991"
     CDS             <1..>466
                     /locus_tag="IV250_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAP8770193.1"
                     /translation="NILDTNRHNIDTEKDRLQDQLLLDNFETIMTNDSIATFVPERVL
                     NLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDSYYVNAEQELYTTLLKA
                     YQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYASSPSTITKDW"
BASE COUNT          192 a           76 c           72 g          126 t
ORIGIN      
        1 ataatatatt agacactaat agacacaata tagacactga aaaagaccgc ctacaagatc
       61 aattgttgtt agacaatttt gagacaatta tgacaaacga cagcattgct acgtttgtcc
      121 ctgaacgagt attaaatttg ataaaaacat tttcttcaag ttacagtgaa gctcaaaaaa
      181 ccgtccagac tattcataat gcaaagaaaa aagctgaaat agaaagtgat atttcgatag
      241 tttttgaaga actcgatagt tattatgtca atgcagaaca agaattatac acgacactgt
      301 taaaagccta tcaaaaatta aaaaccgaaa aagtcgaaaa tatccagaac ctgatttttg
      361 tctatgtaaa aaattggttt atcgaaaaac caatagctgc taaagtatca agtgaaaaac
      421 gtttgaatta tgcaagctcc ccaagcacta ttacgaaaga ctggtt
//
LOCUS       DACDIE010000257          460 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.272, whole genome shotgun sequence.
ACCESSION   DACDIE010000257 DACDIE010000000
VERSION     DACDIE010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.272"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>460
                     /locus_tag="IV250_002992"
     CDS             <1..>460
                     /locus_tag="IV250_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4428 domain-containing protein"
                     /protein_id="HAP8770194.1"
                     /translation="QCAKCGGKIGLTSYKIKDKQIVCGNCMKKAGYGMTTPYKIIRSL
                     NLEDLDKGPSEKRKLNISDYTTQNNHDIGNDRMRTTEEMVEFCLKYGYGKGMTKKWTT
                     HHFNLISDQLNSDEYVIFCFVGLHNYISSTKHDNNYAYALTNKRLIVAQQK"
BASE COUNT          189 a           63 c           75 g          133 t
ORIGIN      
        1 acaatgtgca aagtgcggag gtaaaattgg cttaacttca tataaaatca aagataaaca
       61 aattgtttgc ggcaattgta tgaaaaaagc tggttacggt atgactactc cttataaaat
      121 aataagatct ttgaatttag aagatttaga taagggtcca tcagaaaaaa gaaaattaaa
      181 catatctgat tatactacac aaaacaatca tgatataggt aatgatagaa tgagaactac
      241 tgaagagatg gtagaatttt gtcttaaata cggctatggt aaaggaatga ctaaaaaatg
      301 gacaactcat cattttaact taatatcaga tcaacttaac agtgacgaat acgtaatctt
      361 ctgctttgta ggattgcata actatatctc gtcaactaag catgacaata attatgcata
      421 cgcattaact aataaacgtt taattgtagc tcaacaaaaa
//
LOCUS       DACDIE010000258          460 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.273, whole genome shotgun sequence.
ACCESSION   DACDIE010000258 DACDIE010000000
VERSION     DACDIE010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.273"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(20..91)
                     /locus_tag="IV250_002993"
     tRNA            complement(20..91)
                     /locus_tag="IV250_002993"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56..58),aa:Glu,seq:ttc)
     gene            complement(113..200)
                     /locus_tag="IV250_002994"
     tRNA            complement(113..200)
                     /locus_tag="IV250_002994"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(164..166),aa:Ser,seq:gct)
     gene            complement(225..298)
                     /locus_tag="IV250_002995"
     tRNA            complement(225..298)
                     /locus_tag="IV250_002995"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(262..264),aa:Ile,seq:gat)
     gene            complement(312..382)
                     /locus_tag="IV250_002996"
     tRNA            complement(312..382)
                     /locus_tag="IV250_002996"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(348..350),aa:Gly,seq:tcc)
     gene            complement(384..459)
                     /locus_tag="IV250_002997"
     tRNA            complement(384..459)
                     /locus_tag="IV250_002997"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(424..426),aa:Asp,seq:gtc)
BASE COUNT          132 a          128 c          108 g           92 t
ORIGIN      
        1 taaaactata ttaaattaat gacccgtacg ggattcgaac ccgtgttacc gccgtgaaag
       61 ggcggtgtct taaccgcttg accaacgggc caggtaaaac aaaatattga aacggagaag
      121 gagggatttg aaccctcgcg ccggtttccc gacctacacc cttagcaggg gcgcctcttc
      181 agccacttga gtacttcccc aaaaccaata agattttgta ctaatgggcc taaatggact
      241 cgaaccatcg acctcacgct tatcaggcgt gcgctctaac cagctgagct ataggcccat
      301 aaaaaactaa aagcgggtga cgagaatcga actcgcgaca acagcttgga aggctgtggt
      361 tttaccacta aactacaccc gcatggcggt tccgacggga atcgaacccg cgatctcctg
      421 cgtgacaggc aggcatgtta acccctacac cacggaacca
//
LOCUS       DACDIE010000259          459 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.274, whole genome shotgun sequence.
ACCESSION   DACDIE010000259 DACDIE010000000
VERSION     DACDIE010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.274"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(2..202)
                     /locus_tag="IV250_002998"
     CDS             complement(2..202)
                     /locus_tag="IV250_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8770195.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
BASE COUNT          157 a           69 c           83 g          150 t
ORIGIN      
        1 gttattttgt ataagtagac attgtagttt ctaaactgct atgtcctaaa cgtttcgata
       61 cgctaagtat atttactcct tgataaagta aaatagatgc atgcgtatgt cttagtccat
      121 caataaacag tcgaagagaa aatagggaaa aattgattac taaagaaaaa cttactaggt
      181 cgacggtgta gttgattttc atacttcaat agattcgaat taaacgattt cgattggctt
      241 ataccagtcc tatccaacga aagatatatc gttcttaact ctagcggtat aacttgaagc
      301 tttacgtaaa gccaatggga atgaactgga gatgactttt tattattttt agtaatagct
      361 gatgggttaa tacttacaac tttccttttc aaagtaccat ataaaaaaac ggtggggttg
      421 tttagcgaaa taaaggatct aagccaatag cttagatcc
//
LOCUS       DACDIE010000260          431 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.275, whole genome shotgun sequence.
ACCESSION   DACDIE010000260 DACDIE010000000
VERSION     DACDIE010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..431
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.275"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..198)
                     /locus_tag="IV250_002999"
     CDS             complement(<1..198)
                     /locus_tag="IV250_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HAP8770196.1"
                     /translation="MDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVLSASIPI
                     LIDQASEHRSLLLIVSIASAII"
BASE COUNT          161 a           55 c           47 g          168 t
ORIGIN      
        1 aattattgct gaagcaattg aaacaatcaa tagtaaagac ctgtgttcgg aagcctgatc
       61 aattaatatt gggatactag ctgataagac tatttttata atatttccga taccaattac
      121 tctattataa aatcgaattt ctttttttaa tctatcgatt gtagaatcaa tagattcgat
      181 aaacgctttt tgatccatca ttttctctcc aatcatttta ttaaaatcat taacaaaaat
      241 attataatag gatttaagta agttctaact aatcacatat aaaaataaaa aaacatttgt
      301 attttatcaa agttttatta tacttgtcaa tttgtgtaca tcctaaaata taagctattt
      361 gtttacttct gtagaatata tactttgttg ttagataagt tgcacatgtt tatagaatct
      421 aaataataaa t
//
LOCUS       DACDIE010000261          381 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.280, whole genome shotgun sequence.
ACCESSION   DACDIE010000261 DACDIE010000000
VERSION     DACDIE010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.280"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(21..94)
                     /locus_tag="IV250_003000"
     tRNA            complement(21..94)
                     /locus_tag="IV250_003000"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ile,seq:cat)
     gene            complement(108..181)
                     /locus_tag="IV250_003001"
     tRNA            complement(108..181)
                     /locus_tag="IV250_003001"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(145..147),aa:Met,seq:cat)
     gene            complement(209..281)
                     /locus_tag="IV250_003002"
     tRNA            complement(209..281)
                     /locus_tag="IV250_003002"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(246..248),aa:Ala,seq:tgc)
     gene            complement(301..374)
                     /locus_tag="IV250_003003"
     tRNA            complement(301..374)
                     /locus_tag="IV250_003003"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(338..340),aa:Pro,seq:tgg)
BASE COUNT          103 a          108 c           90 g           80 t
ORIGIN      
        1 tgaaacttac aacgccacaa tggaccttgt aggactcgaa cctacgaccg gacggttatg
       61 agccgtctgc tctaaccaac tgagctaaag gtccaggctc ttaaaaatcg cggcggaggg
      121 gatcgaaccc ccgacctccc gggtatgaac cggacgctct agccagctga gctacaccgc
      181 gaaaaagata ttcattattt aaatgaaatg gagcctagcg ggatcgaacc gctgacctcc
      241 tgcgtgcaaa gcaggcgctc tcccagctga gctaaggccc cgtgtttaaa tttataatta
      301 tcgggaagac aggattcgaa cctgcgaccc cttggtccca aaccaagtgc tctaccaagc
      361 tgagctactt cccgtttaat t
//
LOCUS       DACDIE010000262          362 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.282, whole genome shotgun sequence.
ACCESSION   DACDIE010000262 DACDIE010000000
VERSION     DACDIE010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.282"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..>362)
                     /locus_tag="IV250_003004"
     rRNA            complement(<1..>362)
                     /locus_tag="IV250_003004"
                     /product="16S ribosomal RNA"
BASE COUNT           72 a          113 c           83 g           94 t
ORIGIN      
        1 aaatccggac aacgcttgcc acctacgtat taccgcggct gctggcacgt agttagccgt
       61 ggctttctgg ttagataccg tcaagggatg aacagttact ctcatccttg ttcttctcta
      121 acaacagagt tttacgatcc gaaaaccttc ttcactcacg cggcgttgct cggtcagact
      181 ttcgtccatt gccgaagatt ccctactgct gcctcccgta ggagtttggg ccgtgtctca
      241 gtcccaatgt ggccgatcac cctctcaggt cggctatgca tcgtggcctt ggtgagccgt
      301 tacctcacca actagctaat gcaccgcggg tccatccatc agcgacaccc gaaagcgcct
      361 tt
//
LOCUS       DACDIE010000263          355 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.denovo.283, whole genome shotgun sequence.
ACCESSION   DACDIE010000263 DACDIE010000000
VERSION     DACDIE010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.denovo.283"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>355
                     /locus_tag="IV250_003005"
     rRNA            <1..>355
                     /locus_tag="IV250_003005"
                     /product="16S ribosomal RNA"
BASE COUNT           92 a           75 c          120 g           68 t
ORIGIN      
        1 gaaagccccc ggctcaaccg gggagggtca ttggaaactg ggagacttga gtgcagaaga
       61 ggagagtgga attccatgtg tagcggtgaa atgcgtagat atatggagga acaccagtgg
      121 cgaaggcggc tctctggtct gtaactgacg ctgaggctcg aaagcgtggg gagcaaacag
      181 gattagatac cctggtagtc cacgccgtaa acgatgagtg ctaagtgttg gagggtttcc
      241 gcccttcagt gctgcagcta acgcattaag cactccgcct ggggagtacg accgcaaggt
      301 tgaaactcaa aggaattgac gggggcccgc acaagcggtg gagcatgtgg tttaa
//
LOCUS       DACDIE010000264         9278 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.guided.087, whole genome shotgun sequence.
ACCESSION   DACDIE010000264 DACDIE010000000
VERSION     DACDIE010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9278
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.guided.087"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..834)
                     /locus_tag="IV250_003006"
     CDS             complement(<1..834)
                     /locus_tag="IV250_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP8770197.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVVLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNKHVDLIVALTKANQLDDMFRSIQSIITENTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEAKLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAEGM
                     VRTIVSEFASIAAKEGITLDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YI"
     gene            960..2081
                     /locus_tag="IV250_003007"
     CDS             960..2081
                     /locus_tag="IV250_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP8770198.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALGGTWRTIDGMDLLFRQRKISYLLTNWQGKNTAVIESAQVLGLDLLDKP
                     TDQTIRLASSYALGELIKDTLLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTRKLHLDRRNVLVSKVKVIIAADVTNPYTGQQGAAWQFGRQKGGTDTTLAFLD
                     KQAEKIAQQIKDVWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFELVSQITGLEKQI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDRELEGLFDFLPIV
                     LSIQLGPIDLEEAMEHRQTLEKTEILGQTLSRFFNILPK"
     gene            2339..3841
                     /gene="lsa"
                     /locus_tag="IV250_003008"
     CDS             2339..3841
                     /gene="lsa"
                     /locus_tag="IV250_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lsa family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP8770199.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQAFVYFPQQTKDKERLTYYVLNEIMDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEIAKNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKKVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDALTMNSQVSHHKRLLSVENLQLGYENLLFEPVHF
                     TIEPHQRMAISGPNGAGKSSIIHYLLGTFNGKVVGEKSQPKHLTISYVRQNYEDNRGT
                     LAEFAKKNRVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYI
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLEKI"
     gene            3908..4435
                     /locus_tag="IV250_003009"
     CDS             3908..4435
                     /locus_tag="IV250_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP8770200.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQALAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVVDRLNLPMTADERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECMEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELISYE"
     gene            4647..5708
                     /locus_tag="IV250_003010"
     CDS             4647..5708
                     /locus_tag="IV250_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP8770201.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQNRLIELGSEAKKNKLELMVDISGEALKRAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPNDIDELKEAAADFSQIEAWHNYYPRLETGLDKEWYQEK
                     NQWLKTQGFLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSEHVQEQFASFQKEQTVLLHTEPVDEVFYEYILGKHTNRQDDARDVIRSADARF
                     REIPPIPVRNTAKRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            5711..6607
                     /gene="murQ"
                     /locus_tag="IV250_003011"
     CDS             5711..6607
                     /gene="murQ"
                     /locus_tag="IV250_003011"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAP8770202.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELPSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISEHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEAAQAKLAEANGFVKGTL"
     gene            6621..8603
                     /locus_tag="IV250_003012"
     CDS             6621..8603
                     /locus_tag="IV250_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8770203.1"
                     /translation="MAKNELTLEELAKQIYQGAGGMGNVESIVHCMTRVRMKVINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQEMVDLAGVKLGEKLPSASATTAS
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAGTWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALATGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKAVPQASA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVISSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAADK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(8673..9119)
                     /gene="nrdI"
                     /locus_tag="IV250_003013"
     CDS             complement(8673..9119)
                     /gene="nrdI"
                     /locus_tag="IV250_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAP8770204.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEYEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQRFGFPFLADYELRGTPSDVTRVYQILAEKNK"
BASE COUNT         3014 a         1659 c         2043 g         2562 t
ORIGIN      
        1 gatgtagtcg atttcagtca aacgatggtt tttgatcagg tcttggtaca tagatgggta
       61 atgcaacccg atgttgtcag gatcataagt agaagcaata tgttggtaca cttcttcttg
      121 atctaatgtg attccttctt ttgctgcgat agaagcaaac tcagatacaa tcgttcgaac
      181 catgccctct gccgcttttt gcgcacctaa ttcagcaata ttacaatcca atatcgtgca
      241 taatccattt aatgttccgt ttacacaagc ttttcgccaa atggaataac gaacattatg
      301 actatagatt ggattcaatt ttgcttcttg aaagacttct acaacttttt ttgtaaatgc
      361 ttcgccttct ggttccaagt tctctagttc gatttctcca tcacctaata atgtgacttt
      421 tccgggacca gctagaccag ctgtccacat cgtgatccca aataagatat ttttctttgg
      481 tacatatttt tccagtacat cttcatgacc taatccattc agtaaacaca aaacataagt
      541 attctcagta ataatggatt ggatcgaacg aaacatatca tctaattgat tcgcttttgt
      601 caaggctacg atcaaatcca cgtgtttgtt gctttcgatg atttcttctg gtgaatagat
      661 tggcagtttt gcaactactt ctttgccatt aaagtcagca atcaatccat ttgtacgaat
      721 ggcttcaatg tgagcaggcc agcgatcgat caagacgacc tcatttccgc tttgatgcaa
      781 cattaatccg attcggctgc ccattgctcc ggctccagct attgcgattt tcatgttttt
      841 tctctccttt attcaatgag ttccctttta gtatatagaa aaacagaaga aaatagcatt
      901 gttttgtttg aaaaaagtga aaaaaagtgt cttacttaag aagaaagtga gggatataga
      961 tggatatttt ggcggcaatc gattcattta aaggaagtgc ttcttcattt gaattaaatc
     1021 aggcagttct aaatggttgg gaagaccgta aaggaagaaa aatcaatgtg ccgattgcag
     1081 atggcggaga aaatactgca gaagcaatct attacgcgct cgggggaacc tggcgtacga
     1141 ttgatgggat ggatctctta tttcgtcagc gaaaaatcag ttatttattg acgaactggc
     1201 aaggaaaaaa taccgcagtc attgaatctg cacaagtatt aggattagat ttacttgata
     1261 aaccaacgga tcaaaccatc cgacttgctt ctagttatgc cttgggagag cttatcaaag
     1321 atacactgct tcaagaagtg gaacaaatca ttgtcacttt aggaggaagc ggatgtagcg
     1381 atggtggtct aggattactc caatctttgg gtgcttcttt agaagggtac acagaaggaa
     1441 atcctttgtt atctactaga aaacttcatt tggatcgaag gaacgttctt gtttctaaag
     1501 taaaagtgat catcgctgca gacgtgacca acccatatac aggtcaacaa ggagctgctt
     1561 ggcaatttgg aagacaaaaa ggaggaacgg atacgacgtt agctttcttg gataaacaag
     1621 ctgaaaagat tgctcagcag ataaaagatg tttgggaggt ggatttagat gacatcccag
     1681 gaagcggagc agcaggcggt attggcggag cccttttttt gttaggagcg aagatggtct
     1741 ctggttttga gttggtcagc caaatcactg gattagaaaa acagatcgaa caagcggatg
     1801 tcatcattac aggagaaggg cggattgatc agcaaacaat ccacggaaaa gttccatatg
     1861 gcgtagcagt gcttgcaaaa aaatatcgaa aaccaataat cgctatttgc ggaagtagag
     1921 accgagagct ggaaggactc tttgattttc tccccattgt cttaagcatt caattaggac
     1981 caatcgattt agaggaagca atggaacaca ggcaaacatt agaaaaaaca gaaatcttag
     2041 gacagacact cagcagattt ttcaacattc tcccgaagta gttgacaaaa acaatttctt
     2101 tatgctatcg taatttcaaa tataggacaa agtgagggat ccgtgcattg aaaaattaag
     2161 tcgattgatg aaagctgcat tcagactgta ccttgtggag acaagggagc agtgtgcaga
     2221 ttttcgggat tggcttggtt ctttcgatgt atgggtcttt ttgtgtgccc caacccttgc
     2281 atcctcttta gccagttctg aaaataggac tggtcttttt ttacgaggtg atggaataat
     2341 gtctaaaatc gaaataaaaa atctgacatt cggctatgac agccaaggca cattattatt
     2401 tgaacaagcc aatctaaact ttgacacaca atggaaacta ggacttatcg gacgaaacgg
     2461 tcgaggaaag acaactttac tgaatatcct gcaaaacaaa ctcccttatc aagggcaagt
     2521 gatccatcag caagcgtttg tctatttccc gcagcaaaca aaagataaag agcgtttgac
     2581 ctattatgtg ttaaatgaga ttatggattt tgagatatgg gaaattgaaa gagagctcca
     2641 actgatgcaa acagatcctg aaatcttatg gagagaattc agcacactat caggaggaga
     2701 gaagacaaaa gtcctactgg cactcttatt tgtggatgac actcatttcc cgttaatcga
     2761 tgaaccaacg aatcatttgg atatctctgg cagaaaacaa gtagcggctt acttgaaaaa
     2821 gaaaaaacaa ggattcatcg tggtgagtca tgatcgggga tttatcgatg aagtagtgga
     2881 ccatgtttta gcaatcgaaa agagccaact ggaactttat caagggaatt tctctatcta
     2941 tgaagaacag aaaaaacttc gtgatgaatt tgaaatagct aaaaatgaaa aattgaaaaa
     3001 agaagtcagc aggctaaaga aaacagcagc tgaaaaagca gaatggtctc gttcccgaga
     3061 aggggacaaa acaaagaaaa aagtcggatt catcgatacg gaatctaggc gagtgaataa
     3121 aggagcagta ggcgctgatg ctgcacggac gatgaaacga tctaaagcaa tcgtgaatcg
     3181 gatggaaacc cagatcagtg agaaagaaaa attattaaaa gatatcgagt atatcgatgc
     3241 tttgacgatg aatagccaag tgtctcatca taaacggctg ttaagcgtag aaaatcttca
     3301 attagggtat gaaaatctgt tattcgagcc agttcatttt acgatcgaac ctcatcagcg
     3361 gatggcaatt tcaggtccta acggcgcggg aaagtcatcc attatccatt atcttctggg
     3421 gacattcaac ggcaaggttg taggagaaaa atcccagccc aaacatttga ccattagtta
     3481 cgtacgccaa aattatgaag acaatcgagg gacattggcg gaattcgcga agaaaaaccg
     3541 agtagactat caagcatttt tgaataatct ccgaaagctt gggatggaaa gagatgtttt
     3601 tcataataaa atcgaacaga tgagtatggg acaacggaaa aaagtggaat tggctaaatc
     3661 tttatcccaa ccagctgagc tctatatatg ggatgaacca ttgaattatt tggatgtctt
     3721 caatcaagaa caattagaac aactgatttt gaacgtgaaa cctgctatgt tactagtgga
     3781 acatgatcaa acctttctag ataaagtatc tactgagatt atttctcttg agaaaatcta
     3841 aaccttgtaa tctcaaagac aacaaggtat ggttaaggag aactttgtca acgaaggaga
     3901 gaatcacatg ctttatgtaa cgagacacgg agaaacaact tggaacgcac aaggattagt
     3961 ttgcgggcga gcagatgtgc cgctgactga aaaaggacag atgcaggcac aggcactagc
     4021 agaaaaggta gtagatttgc ctgttccaat cacgaaaatc atccattccc ctcttcaacg
     4081 agcaagagat acagcacaag cagtagttga tcgtctcaat ttgccgatga ccgctgatga
     4141 gcgattggta gaaatggatt ttggggatta tgacggactg ccaagcaaag atgaaaactt
     4201 tcaaaaagcg cgtttagcgt ttgctgtacg tttcccaaat ggagagtcgg tcttagatgt
     4261 gtacgcacgg atcgttcctt tattgaaaga atgcatggaa gacgaagaaa atgtctactt
     4321 gttagtctgt cataatgcct tgataagagt gatcaatgct tattttcatc caatgcctaa
     4381 tgaagggttc tttacattca tggtagacaa tacagaatta atttcttatg agtaattaga
     4441 cttttgtgtt atttaaataa aatgtttcaa ggcactgctt ttgcttagca gtgccttttt
     4501 tgtggctgtg tcaattcttt tggcattata aatccaattt ttagaaataa aatttaatta
     4561 ttttaacaaa agtattgaaa ttttgtttct ttagatatat actctagtca tagaaatgaa
     4621 gacggataca gaaaaagagg gataatatgt ttggcttttc aatatttatg aatgattctt
     4681 tgtcagaaga gaagaaacag tatatccgcc aaatggcatc caatggcttt gtcggtattt
     4741 tcacttccat gcatataccg gaagatgatg tttcggctta tcaaaatcga ttgatcgaac
     4801 taggaagcga agcgaaaaag aataaactag agttgatggt cgatatttca ggagaggcat
     4861 tgaaacgagc tggcttttca ttagaagatc taacaccctt gaaagcaatt ggtgtaacag
     4921 ggttaagaat ggattatcat atttctaatc agcagatcgc cgactggtca catcaattga
     4981 aaatcagttt gaatgccagc acgatcacgc ctaatgatat cgatgagttg aaagaagcag
     5041 cggctgattt ctcacagatt gaagcttggc ataattatta tccacgtctt gaaacagggt
     5101 tggacaaaga atggtatcag gaaaaaaatc aatggctcaa aactcaagga ttccttgtac
     5161 aaggctttgt accaggagat acagagttga gaggtccact gtatcaagga ttaccaactt
     5221 tggaagaaca tcgagggatc catcctctgg caggtgctct tgatctatta gcaagtaaca
     5281 cggatattgt ttatatcgga gatgcaggtc tgtcagaaca cgttcaggag caatttgctt
     5341 ctttccaaaa agaacagaca gtcttgctcc atacggaacc tgtagatgaa gttttttacg
     5401 aatatatatt aggaaaacat acgaatcggc aagatgatgc aagagatgtc atacgaagcg
     5461 cagatgcgcg ttttcgagag attccgccaa tcccagtaag aaatacagct aagcgtatga
     5521 aaggatctat tactttagat aacgaaaagt atttgcgcta tatgggtgag atccaattaa
     5581 ctaaatatga tttgccagcg gatgaaaaag tgaatgtagt agccaaagtc atcaaagaag
     5641 aactaccgct gatcaaccaa atcaaagctg gtatgaacta tcaatttata agaaaagaag
     5701 gaagataaaa atgagcaaaa tcaatttaga gaacctaaca acggaacgca gaaaccaaga
     5761 aacttttgga ttagatgaaa tgagtatttc tcaagcagtc caactcatga acaaagaaga
     5821 tcagaaagtg gccgctgctg ttgaaaaaga actgccaagt atcgagccag tgatcgccaa
     5881 cacgattgaa tcctttaaaa aaggtggtcg cttgatttat atgggagctg gaacgagtgg
     5941 tagacttggt gtattagatg ctgctgaatg tgtaccgaca ttcggtgtag aaccggaaat
     6001 ggtcataggc ctaattgctg gtggagaaga agcaatgact gtagccgtcg aaggagccga
     6061 agattcttat gaattagggc aagaagatct aatcaagctg aatttaacaa aaaatgatat
     6121 agttattggt atcgcagcta gcggtcgtac accatatgtg attggtggat tagattacgc
     6181 aaaatcgatt ggtgctgtta caggaacgat ttcttgcaat aaacaagcag aaatcagcga
     6241 gcatgcggat ttcccaatcg aagtcgatgc aggaccagaa tttttgacag gctctactcg
     6301 attgaagtca ggaacagcac aaaaactgat tttgaatatg atttctacta tcagtatgat
     6361 cggtatcggt aaagtattca ataacttgat ggtcgacgtc aaaccaacaa atgaaaaact
     6421 aattgaacgc tcaaaacgaa tcatcatgca agcgacagaa gctgactacg aaacagctga
     6481 aaagtacttt gaggaagcag atcaaaatgt caaactggct atcgtcatga ttttgacaaa
     6541 tagtgataaa gaggcagctc aagcaaaatt agctgaagca aacggttttg taaaaggaac
     6601 cttatgaggg ggaaaagacc atggcaaaaa acgaattgac tttggaagaa ctggcaaaac
     6661 aaatttatca aggagcaggc ggtatgggca atgttgaaag cattgtccac tgcatgactc
     6721 gtgtacgtat gaaggtcatc aatgatgatc ttgtaaatgt ctcggaatta aaagcgattc
     6781 caggagtact tggcgtagta gaggatgaac agctccaagt catcatcgga cctgggaaag
     6841 tgaacaaagt agcgcaggaa atggttgact tagcaggcgt gaaattagga gaaaaacttc
     6901 cttctgctag tgcaactaca gcaagttctt ctgcatctgg aaaagaccgt gtaaacgaac
     6961 gggcacaaga aatgaaagcg gcacaaaaat cgaaacaaaa accctctaaa atcaaatcgg
     7021 tattaaaaga catctctaat atctttgtac caatgatccc tgcatttgtc ggcactggga
     7081 tcgtggcagg gattgccgca gttttagcaa acttagtcac agctggaaca ctagacgcag
     7141 gaacatggca acaatatatc gatgtgatga acatcttgaa aaacgggatg tttagctatc
     7201 tagttatcta tacaggtatc aatgctgcac aagtttttgg tgcaacgcca acgcttggtg
     7261 gagttatcgg ggctgttgtg atgctgacag ggatgaatcc agaagcacca ctgaaaaact
     7321 tgtttaccgg tgaagctctt gcaactggac aaggcgggat cttaggtgtt atcttcgcag
     7381 tttggttatt gtcaatcgtt gagaaaaaac tgcataaagt agtacctgat gcggtagata
     7441 tcatcgttac gccaactctt tccctgattg ccatcggtct gatcgaaatt ttcttcatca
     7501 tgcctttagc agggttcatt tctgacggca tggtcggtgg gatcaactgg gtacttggtg
     7561 ttggaggagc atttgccggc ttcgttctag gtacattatt cttgccaatg gtcatgttcg
     7621 gactacatca gatcttgacg ccaatccatg tacaaatgat tgatgaaact ggtcgtacct
     7681 tattgcttcc aatccttgcg atggccggag caggacaagt gggtgcagca ttggcattat
     7741 ggatccgctg caaaaaagac aaagaattaa cagaaatgat caaaggggct ttaccagtcg
     7801 gaatcttagg aatcggcgaa ccattgattt acggagtaac attacctcta ggacgtccat
     7861 ttattactgc ttgtatcggt ggaggtatcg gtggggcagt gatcggaatg atcggaaatg
     7921 tcggagcaat cgctattgga ccttcaggag ccgctttgat tccattgatc tctgatggta
     7981 aatggttagg ctatatcctt ggtttattgg ctgcttacgc tggtggcttc gttgctacat
     8041 tcttcttcgg tattcctaaa gaccgtttag aaaaagaaga actagcgacc gaaacagcca
     8101 cagtaaatga agtacctgaa aaagcagtac ctcaagcttc agctgaaagt gtgactatct
     8161 tatcatcagt tgctgacgga acagttgttt ctttagataa agcaagtgac ccagtttttg
     8221 cacaaaaaat gatgggtgaa ggttactttg tagaaccaga aaacggacat atctattccc
     8281 cggttactgg agtaataagt tctgttttcc caacaaaaca tgctatcggc attacgacac
     8341 caaatggctt agaaatcttg cttcacatgg gcatcaacac agttgactta ggcggtacgc
     8401 cattcgatgt gaaagtaaca gaaggacagc aagtgacacc tgatacgctt gtagcagaag
     8461 ctgacttaga agcaatcaaa gcagcagaca aagaaacctc catgatcgtc ttggtaacca
     8521 atatggatcg tgtaaaaaac tttgtcttgg aaaaaacagg taaagcgaaa gcaaaagcac
     8581 ctgtgatgga tgtcaaagct taataagaat agaaacaata gagaacataa aaagaagaag
     8641 ccgaaattta tttttcagct tcttcttttg tgttatttat ttttttctgc caaaatttga
     8701 taaacgcgtg tcacatcaga tggtgttccc cgtaattcat aatctgccaa gaaaggaaag
     8761 ccgaatcttt gtgcgtattg tttggctgtc agacaatatt gattgttgaa gtttttatta
     8821 ccgctaccga ctacaccaag gcaaagttta tgattgtctt cgtattccag atattcgcgc
     8881 atcgtttcgg tcaagatttc tgtgtcaccg ttgtctaacc cattcccgcc gtctaaataa
     8941 gtaggaacga aagtaaaaaa tggttctgtt tctttcgtaa aatcactatt ttcatggatt
     9001 tcttttaaag taacttcagg attgacagga tcttctgcat gcattttttc agcatattct
     9061 gctagatgtt tggcaaaagc acgcgtattt cctgaaatcg atatatataa aatgttcatg
     9121 gatcatcccc caattctttc cctttagtat agcatctttt taagtttttt tggtggtgta
     9181 atttggaaag ggactaggcg ctttgcttgt ataatgagaa aaaagtcggt agaattatat
     9241 ttctattaaa gtttttatta attaagtaaa aataaaaa
//
LOCUS       DACDIE010000265         7820 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.guided.095, whole genome shotgun sequence.
ACCESSION   DACDIE010000265 DACDIE010000000
VERSION     DACDIE010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.guided.095"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(112..1305)
                     /locus_tag="IV250_003014"
     CDS             complement(112..1305)
                     /locus_tag="IV250_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8770205.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDRKYLYKYVDAFGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRANVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVLENDTKKKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQQVMKKRPKAEPFVIDGRGNFLFVNHKGKPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(1384..1590)
                     /locus_tag="IV250_003015"
     CDS             complement(1384..1590)
                     /locus_tag="IV250_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAP8770206.1"
                     /translation="MSNAQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEQVIDKLDAI"
     gene            complement(1583..2725)
                     /locus_tag="IV250_003016"
     CDS             complement(1583..2725)
                     /locus_tag="IV250_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP8770207.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRYPTTDALEIIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CDGVIRRFDLAINDMCGLLDIPVLSEKYRNGGADCRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRGTKAVQAVEELLLTYNPHG
                     LVFYLITDFVQFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDIADVIESEGVFYE"
     gene            3237..3872
                     /locus_tag="IV250_003017"
     CDS             3237..3872
                     /locus_tag="IV250_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8770208.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            4026..4103
                     /locus_tag="IV250_003018"
                     /pseudo
     CDS             4026..4103
                     /locus_tag="IV250_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646598.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            4348..4464
                     /locus_tag="IV250_003019"
     CDS             4348..4464
                     /locus_tag="IV250_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAP8770209.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            4480..6399
                     /gene="tet(M)"
                     /locus_tag="IV250_003020"
     CDS             4480..6399
                     /gene="tet(M)"
                     /locus_tag="IV250_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAP8770210.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            6518..6685
                     /locus_tag="IV250_003021"
     CDS             6518..6685
                     /locus_tag="IV250_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8770211.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(6745..7098)
                     /locus_tag="IV250_003022"
     CDS             complement(6745..7098)
                     /locus_tag="IV250_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8770212.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
BASE COUNT         2377 a         1399 c         1521 g         2523 t
ORIGIN      
        1 cttattttat aggctctacg ggcattttgc ttttgtggta aacctcacat atctaggtct
       61 atcttcttat attttcacta tcaagcgtgg ttaaaatcgt ggtaaatact tctatgcaat
      121 tagacgctga acctctgatt ttgcggtatc tatggacgca tgagcatacc agttcattgt
      181 gatactgata tttgaatgcc ccatgatata ctgtaaatct tttgggttca tgttcttgct
      241 tgccagtctt gtgcagaatg tatggcgtag cgtatgcggt gtgatatgtg gcaatggatt
      301 gtccttgtgg tgcttgttgt atttctttac catacggata aataaggcgt tataatcaat
      361 cgcaactttg ggcttgccct tatgattgac aaataggaag ttgccccgtc cgtctatcac
      421 aaatggttct gcctttggac gttttttcat aacctgttga aatgcctgta tcgtttcttt
      481 gcttaatggt acttgcctta ttccgctttt tgttttaggc gtttcaatat aatagccctg
      541 ttccttgctc tttagtaact ggtggtcgat aatcacaact tcattcttga aatcaatgtc
      601 ggctactgtc agtccgcaca gttccgaaat acgaagtcct gtctttaaca gtatcagcac
      661 atcatcataa tgcttgtgat acacattgtc cgtcttgatg aatgagagta aggcttgttc
      721 ctgttcctct gtcaatgcga ctttcttttt ggtatcattt tctaggactt cactcaactt
      781 gaaatcaaag ggatttttcc tcacataatc atcttgaatg gctatgtaga atgacgcttt
      841 taacgagcgt ttatggttgt taatggtatt gtaggaaaag cctttgtctt tcatgcgtaa
      901 cgcccattcc ttagcgtcag agggtttgat tgtatcaatg ctcctagcac ctaacttgtc
      961 ctctttcaat aaccgcatga gttgttcccg ttgtttctgt gtgcttttct tcacatttgc
     1021 cctctgtgcg ttctgtttgg cataaagttg gcaaagcgtc attttcttgc cagtgctgtc
     1081 gataccgtcc tcaatatccc gtcttatctg ctgttccagt tctcgaagtg agggtttttc
     1141 ccgttttccc tttggtgtcg ggtctgtggg tgtcaatctc caagcataca catattttgt
     1201 gtttccaaat gcgtctacat atttatataa gtattttctg tctgttcgtt ggctctctcc
     1261 agtatgcagg atacgacctt tgttatcccg tcttttttct ttcatggtgt ctgctccttt
     1321 ccttgttgga aagagccttg atatgacttg tgttcatcat aacacataca aggctcattt
     1381 gcattagata gcgtccaatt tatcaatgac ctgttcaaac tgtcggcgtt taatctgtat
     1441 gcggttgcca ttcataatga gccagccagc gtccttgttt tcctctgcca atcgtcttaa
     1501 cttgttttct ccgatacgga aatactttga cgcttcttca atggtaaggg tgtatttttc
     1561 ccatacggga atatcctgtg cattactcat aaaacacccc ctcactttcg attacgtctg
     1621 ctatgtccgt acacatctgt tccacaagca tttcctgctt ttcagagagg tgggtacatt
     1681 catcaatcat tttcatgtag tttgagcctg tcagcctgtc gatttcctct atcatcacga
     1741 ttgacggcat gacctgtctt tccagccatt gcagagtgcg ttccatattg acaggtacgg
     1801 ggttcatttc aaaaggtaaa ctgtcatggg aaatgaatat ctcccacagc ggatagtcgg
     1861 gaaattgcac aaaatcagta ataaggtaaa acaccagccc atgagggtta tatgtcaata
     1921 acaattcctc aactgcctgt actgcttttg taccacgcag acgaatttca aaacgattga
     1981 taatatccgt atgtttcttt ttggttgcct gttccttttg tttttcatac aggcagaagt
     2041 attttgtgct tgcctttgaa ccgatataaa gggtgcttgc taaattacgg ttttttccgc
     2101 ttagttttcc accctgcaca ttttcataat ttttacagcg acaatccgca ccgccatttc
     2161 tgtatttttc agacaaaacg ggaatatcca acagaccaca catatcattg attgccaagt
     2221 caaaacgtcg gataacaccg tcacagtcaa gacagcggtt taaaaatgaa taccaatctc
     2281 tgttctgtgc ctgcaagaca tattccatgt tgcgtgagcc tgcccctttt aattccaaga
     2341 atacgcccat gtgttcgtca tcagaagcca tgaccttgat ttctccgtag gcgtaatgct
     2401 ctttatagcc ataataccca tagtcataat ggataaaata atctgctttc atgccaagca
     2461 cctttcggat aatttccaat gcgtcagtcg ttggataacg gacagacaga taatcaacga
     2521 gcagataaaa aggcttatcg cagatataat aattcagaca acgcaggata atattttgtg
     2581 tttccttgct ggcgttggct tttccgttct caaagcggtt gatactatat cttgatacat
     2641 gggtaagact tgcaagttct ccttgtgaaa gaccgcttgc aagacgtttg ttcttcattt
     2701 caagtgcaaa ttctaaattg ttcattgtat tcctccaatc ccacaatagc cttgaaatac
     2761 ctgtaaacct ctattcaaaa agtgtgatat gcactatctt cttaaaactt ctcattacta
     2821 ctcacaactt tgggattgta tggtttttga cttgtctttt gtgaaatagt gcccccctgt
     2881 tagataacgg gggcacagac aaggctcgca ggctcgccag ccgacaaagc atttcttccc
     2941 cttgacacaa ggtgtcatgg cggtaagcct gagaggcttc cgccaccgtg tcagaaatac
     3001 tttgtcagca agttctgttt tgccttttct aacaagttcc atttcaaccg atattcatat
     3061 ctacggattt aatcagatag gtatttcttt ttgaatgttt ttatatttca ttctattgtg
     3121 cttattcgat tacaagaaca tagtaattct tatatgctta tttgtcaata tctttttttg
     3181 cctttacaaa tcatattaag catgatagac ttataatagc gaagatataa cgagatatgg
     3241 agggttatgc aatgaaaata agccatttag gaaataatat acagaccata agaaaattta
     3301 ggggaatgaa acaacaggaa cttgcggaca aaatcggtat caatatgcag agcctttcca
     3361 agattgaaag agggttgaac tatcctgcct atgaaacgct ggaaaagata atggaagtgc
     3421 tggacgttac gcccaatgaa ttgttgtcgg gagaatggaa gtatgtcaat caatctgaaa
     3481 aggaagtctg tcagttttta agagccgaag aacgtctaaa tgcagaactg aaacatggac
     3541 actatgataa cttctttgac agcgaggaag aatggctgga atatgagtta gaaaagttac
     3601 gggaatatat tacagactat atcaacggga aaagcattga agcgtccgac ctttacccaa
     3661 tcaaagaatt tatccagcat ttgaagtttc aaaaattatt agaccgctat gatgatttat
     3721 acagtatgga tatgtttggg gaaagcatag agggacataa gtataagaca ccttatcaag
     3781 tcgtgaaaat gataaacccg aactcaaaag aagatatgga actgttacga gaagtattaa
     3841 ggaataacaa ttttgatgat gaggacgagt aacttttttc ctcttgcgag aagatacagc
     3901 aaacaaaaag cccgatcaag agtgcaaagc accaccaatg gtggcaaagc tcttgacagg
     3961 gctttttctt ttctgttgtg ggcaatcaag aggaagaaag ggataaaatc tgatttgtgg
     4021 taaatcaggc aaaggcaacg caggtatctc aatttgattt ggtacttgaa aagaacggga
     4081 gtaattggaa gattatagaa taacaaatat tggtacatta ttacagctat tttgtaatca
     4141 cgtactctct ttgataaaaa attggagatt cctttacaaa tatgctctta cgtgctatta
     4201 tttaagtatc tatttaaaag gagttaataa atatgcggca aggtattctt aaataaactg
     4261 tcaatttgat agtgggaaca aataattgga tgtccttttt taggagggct tagttttttg
     4321 tacccagttt aagaatacct ttatcatgtg attctaaagt atccggagaa tatctgtatg
     4381 ctttgtatgc ctatggttat gcataaaaat cccagtgata aaagtattta tcactgggat
     4441 ttttatgccc ttttgggttt ttgaatggag gaaaatcaca tgaaaattat taatattgga
     4501 gttttagctc atgttgatgc aggaaaaact accttaacag aaagcttatt atataacagt
     4561 ggagcgatta cagaattagg aagcgtggac aaaggtacaa cgaggacgga taatacgctt
     4621 ttagaacgtc agagaggaat tacaattcag acaggaataa cctcttttca gtgggaaaat
     4681 acgaaggtga acatcataga cacgccagga catatggatt tcttagcaga agtatatcgt
     4741 tcattatcag ttttagatgg ggcaattcta ctgatttctg caaaagatgg cgtacaagca
     4801 caaactcgta tattatttca tgcacttagg aaaatgggga ttcccacaat cttttttatc
     4861 aataagattg accaaaatgg aattgattta tcaacggttt atcaggatat taaagagaaa
     4921 ctttctgccg aaattgtaat caaacagaag gtagaactgt atcctaatat gtgtgtgacg
     4981 aactttaccg aatctgaaca atgggatacg gtaatagagg gaaacgatga ccttttagag
     5041 aaatatatgt ccggtaaatc attagaagca ttggaactcg aacaagagga aagcataaga
     5101 tttcagaatt gttctctgtt ccctctttat catggaagtg caaaaagtaa tatagggatt
     5161 gataacctta tagaagttat tactaataaa ttttattcat caacacatcg aggtccgtct
     5221 gaactttgcg gaaatgtttt caaaattgaa tatacaaaaa aaagacaacg tcttgcatat
     5281 atacgccttt atagtggagt actacattta cgagattcgg ttagagtatc agaaaaagaa
     5341 aaaataaaag ttacagaaat gtatacttca ataaatggtg aattatgtaa gattgataga
     5401 gcttattctg gagaaattgt tattttgcaa aatgagtttt tgaagttaaa tagtgttctt
     5461 ggagatacaa aactattgcc acagagaaaa aagattgaaa atccgcaccc tctactacaa
     5521 acaactgttg aaccgagtaa acctgaacag agagaaatgt tgcttgatgc ccttttggaa
     5581 atctcagata gtgatccgct tctacgatat tacgtggatt ctacgacaca tgaaattata
     5641 ctttctttct tagggaaagt acaaatggaa gtgattagtg cactgttgca agaaaagtat
     5701 catgtggaga tagaactaaa agagcctaca gtcatttata tggagagacc gttaaaaaat
     5761 gcagaatata ccattcacat cgaagtgccg ccaaatcctt tctgggcttc cattggttta
     5821 tctgtatcac cgcttccgtt gggaagtgga atgcagtatg agagctcggt ttctcttgga
     5881 tacttaaatc aatcgtttca aaatgcagtt atggagggga tacgctatgg ctgtgaacaa
     5941 ggattgtatg gttggaatgt gacggactgt aaaatctgtt ttaagtatgg cttatactat
     6001 agccctgtta gtaccccagc agattttcgg atgcttgctc ctattgtatt ggaacaagtc
     6061 ttaaaaaaag ctggaacaga attgttagag ccatatctta gttttaaaat ttatgcgcca
     6121 caggaatatc tttcacgagc atacaacgat gctcctaaat attgtgcgaa catcgtagac
     6181 actcaattga aaaataatga ggtcattctt agtggagaaa tccctgctcg gtgtattcaa
     6241 gaatatcgta gtgatttaac tttctttaca aatggacgta gtgtttgttt aacagagtta
     6301 aaagggtacc atgttactac cggtgaacct gtttgccagc cccgtcgtcc aaatagtcgg
     6361 atagataaag tacgatatat gttcaataaa ataacttagt gtattttatg ttgttatata
     6421 aatatggttt cttgttaaat aagatgaaat attttttaat aaagatttga attaaagtgt
     6481 aaaggaggag atagttatta taaactacaa gtggatattg tgtcctgtat gtggaaataa
     6541 aacacgatta aagataaggg aagatactga attaaaaaaa ttccccctct attgtccgaa
     6601 atgcagacaa gaaaatttaa ttgaaataaa gcagttcaaa gtaactgtga ttacagagcc
     6661 agacgcaaag acgcagagcc gataaaatga gattaataca atctcatttt atcggctctt
     6721 tccgttatgt atggattctt ttaattagtc ttcgatgttt cttgcttcgt tgataccgct
     6781 ggctaaagat tccattaagg atagttcttt gtctgtaaag ctatccatgt atttctctat
     6841 ctgtaatcgt cgggtgcttt ttaccaagtt attagcaggt aagaaaaatt catcaacgga
     6901 aacatgaagt aacgatacaa ggtcataaag aacttgtatg ctggggtgtt gccctttatt
     6961 ttcaatatta gttaagtacc gtgggtcaat ttcaatcaat gctcccactt gttcacgagt
     7021 taaacctcgt ttcaatcgag cttctttaat ggctaaacca aaggctctaa aatcatattt
     7081 atcttctttt ttacgcatag tagaccacct ctatacattt tattgttcct actgaattaa
     7141 aaacaggtat agaaaaacgt gttatatggt ttataggttt atatttaata aaaagcacta
     7201 ctaaacgcca ataaaaaaaa ccgttatatg gtagtgctat ttacgctgtt aaaatattgt
     7261 atattacttc caaatggcgg tttgttggag gtcaacgtcg ccatgaagta catcatatac
     7321 aataaatttc cttacattgg gttcttgtca aaaaaagtcg tctatctgca atagataagt
     7381 acgtccacca atgtggtttt ataaatcata tagatagaat aacagaagca tgtaaacaga
     7441 gaaataaatc tgtttatatg cttttttggc tattcagaac ttttttacaa agtttattta
     7501 tcagtaatgc aacaaatccc cctttcacat tgggactaag agtgaaagga gataaacgag
     7561 caaggctcac ttcctttcct agacagaaag ggggtgagat tatgaggaca tcttctttcg
     7621 agcatatcgt cagattacag ttcaattctt taatgctgat tgtcattaag aacaaagtga
     7681 aaagtcgaaa cagacagctt gcaagacatt ctaaacgtga agtattgttt tgtgaaatag
     7741 ccgaaacgaa acaagttgaa cgtggaacga ttgatacctg tttttatgat tgtgtatcat
     7801 ttaaagtctt gcactttaca
//
LOCUS       DACDIE010000266         6870 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.guided.102, whole genome shotgun sequence.
ACCESSION   DACDIE010000266 DACDIE010000000
VERSION     DACDIE010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6870
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.guided.102"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..475
                     /locus_tag="IV250_003023"
     CDS             <1..475
                     /locus_tag="IV250_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8770213.1"
                     /translation="TGKKGSSEETGVPGMYKKVENPFVERTNWDWEIDPEGLRIGLRR
                     ITSRYRMPVLITENGLGEYDKLTEDKQIHDDYRINYLQSHVKAIKEAISDGAEVLGYC
                     TWSYTDLLSWLNGYQKRYGFVYVDQDETQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            664..3111
                     /locus_tag="IV250_003024"
     CDS             664..3111
                     /locus_tag="IV250_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAP8770214.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            3341..4408
                     /gene="rlmN"
                     /locus_tag="IV250_003025"
     CDS             3341..4408
                     /gene="rlmN"
                     /locus_tag="IV250_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAP8770215.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(4601..5704)
                     /locus_tag="IV250_003026"
     CDS             complement(4601..5704)
                     /locus_tag="IV250_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP8770216.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            6257..6805
                     /gene="aac(6')"
                     /locus_tag="IV250_003027"
     CDS             6257..6805
                     /gene="aac(6')"
                     /locus_tag="IV250_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP8770217.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         2314 a         1176 c         1483 g         1897 t
ORIGIN      
        1 gacaggaaaa aaaggttcat cagaagaaac tggagttcct ggaatgtata aaaaagttga
       61 gaatccattt gtcgaacgaa caaattggga ctgggagatt gacccagaag gattaaggat
      121 cggactgcgt cgtatcacaa gccgttaccg catgccggta ctgattacgg aaaatggctt
      181 aggagagtat gacaagttga cagaagataa acaaatccat gacgactatc gaatcaatta
      241 cttacaaagc cacgtcaaag ccatcaaaga agcaatttcg gatggagcag aagtcctagg
      301 ctattgcacc tggtcttata cagatttgct tagctggctg aacggttatc aaaagcgata
      361 tggatttgta tatgttgatc aagacgaaac acaagaagga tctttggaac gatataagaa
      421 agacagttat tactggtacc aaaaactgat tgaagaaaat ggagagaacc tttagttcac
      481 atacttaaaa caagaaggta cggctggata tggccgtgcc ttttcttgat aaaatcattt
      541 agaaattaag aaaaactgta aaataagctg aaaaatgaaa gaaagcgtca gttctttcaa
      601 acaaaacttt agttccctta cttttttttg tataatgaaa cttgatgaaa tgagggataa
      661 taaatggcac aaaagaaacg gcctactaaa aaaaggaaaa agacgaaaaa aacgaaaaag
      721 caaaaacagc agcaggaaca aatgctgacg attggtgttg ggtttgcctt gattttattt
      781 gctgtcttcg gctttttaaa attaggtttt ttaggaatat tgattgcaaa cggctttcgg
      841 atcattgcag gaaatactta ccaaatcctc tgtcttttat tagccgtatt aggattttgg
      901 attgtcatta agaatacaga gttttcaatt ggtaaaaacc gacgctggtt tggcggcata
      961 ctcttttatt tcggtatctt attattgtta catgcccatt tatttggcaa actccatact
     1021 ggggaaccaa atatcatggg gacgacatgg gatttgttag ccagtgatat aaaacaaagt
     1081 caagtagaca ataacgttgg tggtgggatg attggagcaa tcctttatca ttttacttac
     1141 ttcttgattg cacagcctgg aagttattta gcagcagtgc tattactggc aggaggagca
     1201 ttcctgttca gtaatctgga aggctatcag ctgttaaatg gtatccaatc aattggagaa
     1261 cgagtacagg aattattgga aggtgatcct gccaagcaag ctcgaaagca agcggcaaag
     1321 gaagagagaa tgaaacagcg cgcagaagcg aaagaagcaa ggcgcttagc tgcaaaagaa
     1381 gctgcagaaa aagaagctgc agaatatgag aagaaaaaag cggtgagcca gcaaagggaa
     1441 aatactgctg atgaatggca ggaaccagaa aatacagagc cggagcagct aagttttgtt
     1501 ccaatcgaca gttttcaaga aaatatccat cctgcgaatc tagagaaacc agtaccagat
     1561 acaccaaaac agaccaatac agcagaaggt ttttcagatg agttgccaga ggatgacggt
     1621 acctcattag aatttgagat tgaagcagaa caggaaaatc aagactatga attaccgaca
     1681 gttgatttgc ttgattctat accaactgtg gatcaaagtg atgagtataa aaaaattgaa
     1741 aaaaatatcg gcgtattaga acaaactttc caaagtttcg gtgtcgatgc caaggtcgtc
     1801 aaagccagct taggaccatc cgtcacaaaa ttcgaagtcc agccggctgt aggggtaaaa
     1861 gtaagtaaaa ttgtgaactt aacagatgat atcgcgcttg ctttagcggc gaaagatgtc
     1921 cggatggaag caccgattcc aggtaaatca ctgattggta ttgaagtacc aaatggaaag
     1981 atcagtatgg tttcctttag agaaatcatc gaagcacaac caaaccaccc agataaatta
     2041 ctagaagtac ctttaggacg tgatgtttct ggacgggtac aaacggctga tctttcgaag
     2101 atgcctcact tactcgtggc tggttcgact ggtagtggga agtcagtagc aattaatggg
     2161 attatcacaa gtatattaat gcgtgctaag ccccatgaag taaaactgat gatgatcgat
     2221 ccgaaaatgg ttgaattgaa tatgtacaac ggcatacctc atctgttgac gccggtcgtg
     2281 acgaacccta gaaaagcagc gcaagcctta caaaaagtag tacaagaaat ggaagaacga
     2341 tatgaaaaat tcgcggcaac cggagtaaga aatatttctg gttacaatga atttgttcaa
     2401 cagaaaaact tggaaaacgg aacgaaacat ccaaccttac cattcatcgt agtgatcgta
     2461 gatgaattag ctgatttgat gatggtagcc agcaatgaag ttgaagatgc gattattcgt
     2521 ttagcgcaaa tggctcgggc agcggggatt catatgatcc tagctactca acgtccaagt
     2581 gtggatgtta tcacaggaat catcaaagca aatgtacctt ccagaatggc atttgcggta
     2641 tcaagcggta cagattctcg gacgatcatt gattctaatg gggcagaaaa gctcttaggg
     2701 cgaggagata tgctattctt gccaatgggt gagaataaac cgatccgtgt gcaaggagca
     2761 ttcatttctg accacgaagt agaacgggtc gttcaatttg ttacagatca gcaagaagct
     2821 cattatgaag agaaaatgat gccgacagat gaagttgaaa cgacaggtgc tccagaacag
     2881 ccacaagatg agctgtttga agaagcaaaa gcacttgtcg ttgaaatgca aacggcaagt
     2941 atttcccttc tccaaagaag atttcggatc ggttacaacc gagcagcacg tttagtagat
     3001 gaactggaag ctcatggagt tgttggcccg tcagaaggaa gcaagcctcg gaaagtattc
     3061 attgagcagg aagaaacatc agaaacgttg ccggacgata atgacatgta aaacttgtgt
     3121 gatgaagggt tgaaaaacga ataaccaata aaaagacaag gcatgatgat accaaaagaa
     3181 aaaatggttt ttttccatct tttctaatac ggtatgatgt cttgtctttt tagtttttgt
     3241 gagattaaag ttctattcga tttatttgtg aagtattgca ttaaggaaat gaatcatgta
     3301 tagtatgtag cgaaactttg agaaaaagag ggaaaatact ttggaaaaac agtccattta
     3361 tggcttgacg aatgaagaat taatcaattg gtttatagaa aacggcgaga aaaaattccg
     3421 agctgcccaa gtttgggaat ggttgtatca aaaacgtgta agtaatttca cagaaatgac
     3481 gaatctatcg aaacaattga tcgaaaaatt atcggagcat tttatcatca atcctctaaa
     3541 acagatggtc gtccaagaag cttcggatgg tacagtaaaa tatttgtttg aactccctga
     3601 taaaaacatg attgagactg tactgatgag acaagaatat ggcttgtcag tttgtgtgac
     3661 gacacaagta ggatgtaata tcggctgtac gttttgtgca agcgggctgc tcaaaaagaa
     3721 ccgtgatctg actgctggag aaattgttgc acagatcatg atggtccaac attattttga
     3781 tgagcgcaat cttggcgaac gcgtttcaca tgtcgttgta atggggatcg gtgagccatt
     3841 tgataattac gacaatgtaa tggacttttt gcatatcatc aatgatgcca aaggattggc
     3901 aattggtgct cggcacatta cagtttccac tagtggatta gcacacaaaa tcaaggaatt
     3961 cgctaataac ggcttgcaag tcaatctggc tatttccttg cacgcgccga ataatgaggt
     4021 gcgaacatcg atcatgcgga tcaaccgaag tttcccaatt gagaaattga tggaagctgt
     4081 ggatgagtat ttagagaaaa caaaccgtcg aatcacgttt gaatatatca tgttgaatca
     4141 agtaaatgat agaccagagc atgcgcaaca attagcagat ctattaaaag ataagaaaaa
     4201 attagcatat gtcaacttga ttccatataa tccagtcagt gaacatgatc aatattctcg
     4261 cagtccaaaa gctgatgtat tgagatttta tgatgtattg aagaaaaacg gcgtaaactg
     4321 tgtgatacgt aaagaacatg gtacagatat tgatgcagct tgcggacaat tgcgaagcaa
     4381 gcaaatgaaa aaaacagttg ccaaataatc gtatcctact cgttttttca attaccataa
     4441 aaggagcagt tttcttaatt aaggaactgt tcaacttaaa taagagaaaa gattctatag
     4501 cgaaagctat aaagcttttt ttgacaagaa aaaccctcat caagcaaaga aatgtcctca
     4561 atttgatgac agcctgcttg atgggggttt tagcaattat ttaactgatt ttcttatatc
     4621 cttgctggct ttcttgtgta gcatgataag tttctacgtc aagaatggcc tgtttaaatt
     4681 tttctcgggt gatttcataa ggcacatgtt taatatcatt tgttgccatg gctttatcta
     4741 agattgcttc taaatcggtc gtgacattga gattcatgtc ggttaacgat actggaagtt
     4801 ggttcttttt cataaacaca tagattttat caaacaaatc gaactgctga tccatcatca
     4861 ataaggtcag tacaccataa ctgacagatg ctccgtgaag gtatccctcc attttttcta
     4921 gaacagtcgt accgtaatag aatgagtgtg ccatagcaga attatagtta ttgttaacta
     4981 aaacagaaac caatccggta gtaacaatga tatccgaaac gacttgttca attgcatatg
     5041 agggacgatc gtcggcacta tctttcaatg cttgcgtacc ataagtcaag agacgatcaa
     5101 tgcacatttt tccgatttgg attcctaatc tatccgtgta gcctagatca tcgccacgag
     5161 cagaaaaagt agattcgact tctttggata aagcatcgcc aatacctgcc cataagtatt
     5221 tatcaggtgc ttcggcaata attgctgtat caatgaaggt gtgtacaggt ggttttgaag
     5281 gaaagaagag tccgtccatt acgccttctt cgttatagat gacacatacc gcagtgacgg
     5341 gcgaacagtt cgaaccaata gtgggaaagg tgaagactgg tttagctagt ttttcaccaa
     5401 tgacttttac agtatcgcaa gcacggccac caccgactgc aaacaggacg tctgcttttt
     5461 ggacactttc atgctggatc aactgttcaa tatttccata agttgaatta cccccgtacc
     5521 agagactggc gacaacctct atttcctgtg tcaaagctac ttttattttg ttttctgcct
     5581 ttgaaagtgc agtcttccct ccaatcagca ctacttttgt cccatattct ttgacgattc
     5641 catttgcttt ttcatacgca tctgttccaa tggtataatt tggtaagaaa acactttgac
     5701 tcatattcat ttccttcttt cttcgttgaa acgggttaaa attttaaaga gtgatcgatc
     5761 tgactgcggc tgtagatgga gtagaggtga acctaagaca ggataagtga tgaataaggt
     5821 ggcatgaaca ccttgaaatc atcaataagt atcctaaaaa cgaacaagta caaaaacatt
     5881 gaaaacgaca ataatgaatt tttgttcctc ttagtatttc taccgattcc ctttgctttt
     5941 ttgtaattat ctaaaaattc taaaaattaa gattattata tgaaaacagg ttttatttgt
     6001 caattcataa ttggtttttt ttaaagcaaa agataatcta tatcatgatg aacaaacaga
     6061 gttttttcct ccaattgatg gctttcctga tctttcctat tttgaataaa gtgtttctgt
     6121 ataatttttt tattttataa gtaagataaa taatgaaaga atagagatac tgattggtag
     6181 ctaaagattt ttgagcgaga acatttcgtt ttttcaaaag gactgttaga ataagcaaga
     6241 acaaggggga agacgtatga taatcagtga atttgaccgt aataatccag tattgaaaga
     6301 tcagctttct gatttactga gactgacttg gccggaagaa tatggagaca gctcggcaga
     6361 agaagtagaa gaaatgatga atccagaacg aatcgcggta gcagcggtag accaagatga
     6421 gttagtagga tttattggtg caatccctca atacggtatc acaggttggg aattgcatcc
     6481 attagttgta gaaagctccc gacgaaagaa ccaaataggt actcgattag tcaattactt
     6541 agaaaaagaa gtagcttcca gaggaggaat cacgatttat ttaggtacgg atgatttaga
     6601 ccatggaaca acgttaagtc aaacggacct gtatgagcat acatttgata aagtggcttc
     6661 catccagaac cttcgtgaac atccgtatga attctatgaa aaattaggtt ataaaatcgt
     6721 aggtgtctta ccaaatgcaa atggctggga caaaccggat atttggatgg caaaaacgat
     6781 tattcctcga ccaaattctc aataataaaa agaggttgtg acagaagtaa tcagcttcaa
     6841 gaaataagaa ggaatttacg aaaattactt
//
LOCUS       DACDIE010000267         2466 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.guided.148, whole genome shotgun sequence.
ACCESSION   DACDIE010000267 DACDIE010000000
VERSION     DACDIE010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.guided.148"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..259
                     /locus_tag="IV250_003028"
     CDS             <1..259
                     /locus_tag="IV250_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019299635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP8770218.1"
                     /translation="SFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPIGGFEADQL
                     AEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            613..2091
                     /gene="msr"
                     /locus_tag="IV250_003029"
     CDS             613..2091
                     /gene="msr"
                     /locus_tag="IV250_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP8770219.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            2138..>2466
                     /locus_tag="IV250_003030"
     CDS             2138..>2466
                     /locus_tag="IV250_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP8770220.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQ"
BASE COUNT          834 a          391 c          533 g          708 t
ORIGIN      
        1 gagtttttca agagaagaaa tgaacgatgt agtcaaagtc gattctagct tagcggctat
       61 gcaattcatg ttagttgcgc gtgatcatgg ctatgaaaca aatccaattg gtggattcga
      121 agcagatcag ttggcagagg catttggttt ggataaagac cgttatgttc cagttattat
      181 tttatcaata ggaaaagcgg tagaagaagg ttacgagtct gttcgtttag caccagaaaa
      241 gatcactatg ttcgaataaa aggttttcag ttactattca gtagctattg ctttttttga
      301 atcgtgtgtg gtaacctaat aaaaaaggag gtcggaaaaa tgactgcatc gatgaaatta
      361 cgtttcgaac ttttgaataa caactaaata aattcaaaag acatgtataa acaacaattt
      421 catggatgga gtatgtccat tttttcgaac gacagtagaa taggtgaaag ctatgacaga
      481 tttggtgatt tagtcatagt tatttcgttc agagtaggaa aagatgtgat gcagtttttt
      541 tgcaaacaga tttttctgct cttttttagt atcgataatt gttgttttac atgaaatagg
      601 agtgtaaaac atatggaaaa tttagcagta aatataacaa atctacaagt cagctttggc
      661 aaccagctag aactatctat tgattctctt cgtgtctatc agcaagatcg tatagggatc
      721 ataggagaaa atggtgtcgg taaatcgact ttgctcaaac taatagccgg tgaacttttt
      781 cccgatcatg ggaaaatcca aacagagatc accttcaact acctgcctca attaacctat
      841 ctcgctgaag caaaggacct aaatttggaa ttagccagtc atttccagtt aagactggaa
      901 gaaacttcag agcggaaatg gagcggaggg gaagaacgaa agatcgagtt gatacgtctt
      961 ctttcttctt atgaacaagg gatgcttcta gacgagccaa caacccatct agatagaaaa
     1021 agtattgatc gactgattga agagcttcgt tattattatg gtacgctggt ttttgttagt
     1081 catgatcgct attttctaga tgagctagca tcgaaaatct gggaagtaaa agacggagaa
     1141 atccgagagt tttcggggaa ttatagtgcc tatctcactc aaaaggaatt ggagaaaaag
     1201 actcagctac gagaagcaga gtcgatcatg aaagagaaaa aacgattgga aaaatcgatc
     1261 caagaaaaga aaaaacaagc ggaaaagttg gaaaaagtgt ccagtaaaaa gaaaaagcaa
     1321 caaatcagac tggatcggtt gtcttcctct aaacaaaaag acagtgtaca aaaagccatc
     1381 caaaagaatg cgaaaacatt agagagaaga ctccaaaaaa taggagaaac aaccaagccg
     1441 cagcagatga aacaaatccg ttttccagta ccaaaatctc ttgaactcca cagccgctat
     1501 cccatcatgg gacaaaatgt ccaattggaa agaagtggga gaacattact ggtaaatggt
     1561 gattttcagt tttctttagg taaaaaaatc gcgattgtcg gtgaaaatgg ttcaggtaag
     1621 acaaccttat tagaacatat ccgcaaacaa ggagaaggaa tccttctctc tccgaaagta
     1681 agctttcaag tatatcagca aaagggttat caaatgacat ctgaagaatc catcattcgt
     1741 tttgtcatga gacaaacaga gttttcagaa tcgcttgtcc gtagtttgct gaatcactta
     1801 gggtttgctc aggaaacttt gacgaaaccg ttatgtacat taagtggggg agaagcgacc
     1861 cgtctgacga ttgctttgct ttttactaag ccaagtaatg tgttgctgtt agatgaaccg
     1921 actaatttta ttgatatggc aacgatcgaa gctttagaga agctaatgca aatatatccg
     1981 ggaacgattt tgtttacttc tcatgattct tactttgtcg agcgtacggc tgatgaagtt
     2041 tatgaaataa aagggcagaa aataaaaaaa gtacttacga gaaattttta aatgtgctac
     2101 gatgattttc gtgacaaatt taaaggagtg gtacagattg cctacaaata aaaaagaagg
     2161 aataattttt acaacaatga tgtgtttttt gatggtcttg gggatgagcg cgtataattt
     2221 atggctgcat caagatcttc atttatcaag tttgttgatt ggtctagtgc ctggattcgt
     2281 ggtggctttc attttggatg tcttcatcat cggtgtgctg gcgaaaaaaa ttgcctttaa
     2341 actaccagta aatccagaaa gcaaggtcca aatgatctta actatttcat gcttgatggt
     2401 tttaggaatg gtgacatgta tgtctttatt cggtgtgatc atggaacaag gaatacctga
     2461 tcaatt
//
LOCUS       DACDIE010000268         1349 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.guided.181, whole genome shotgun sequence.
ACCESSION   DACDIE010000268 DACDIE010000000
VERSION     DACDIE010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.guided.181"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            46..297
                     /locus_tag="IV250_003031"
                     /pseudo
     CDS             46..297
                     /locus_tag="IV250_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            390..1184
                     /locus_tag="IV250_003032"
                     /note="aph(3')-IIIa"
     CDS             390..1184
                     /locus_tag="IV250_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAP8770221.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          424 a          234 c          322 g          369 t
ORIGIN      
        1 cattttcctc taggaaaaaa ttaactagca ccacgggtta tcttttatat agaagatatc
       61 gccgtatgta aggatttcag ggggcaaggc ataggcagcg cgcttatcaa tatatctata
      121 gaatgggcaa agcataaaaa cttgcatgga ctaatgcttg aaacccagga caataacctt
      181 atagcttgta aattctatca taattgtggt ttcaaaatcg gctccgtcga tactatgtta
      241 tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat
      301 gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa
      361 tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga
      421 aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg
      481 tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata
      541 aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc
      601 tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga
      661 gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga
      721 ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc
      781 cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc
      841 tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc
      901 tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc
      961 tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga
     1021 gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg
     1081 atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt
     1141 gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta gatttagatg
     1201 tctaaaaagc tttaactaca agctttttag acatctaatc ttttctgaag tacatccgca
     1261 actgtccata ctctgatgtt ttatatcttt tctaaaagtt cgctagatag gggtcccgag
     1321 cgcctacgag gaatttgtat cgataagaa
//
LOCUS       DACDIE010000269         1082 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.guided.199, whole genome shotgun sequence.
ACCESSION   DACDIE010000269 DACDIE010000000
VERSION     DACDIE010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.guided.199"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..55
                     /locus_tag="IV250_003033"
     CDS             <1..55
                     /locus_tag="IV250_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HAP8770222.1"
                     /translation="KTSTVLKQTKNSDYADK"
     gene            180..917
                     /gene="erm(B)"
                     /locus_tag="IV250_003034"
     CDS             180..917
                     /gene="erm(B)"
                     /locus_tag="IV250_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAP8770223.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLNTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          416 a          181 c          158 g          327 t
ORIGIN      
        1 taaaacatct actgttttga aacagactaa aaacagtgat tacgcagata aataaatacg
       61 ttagattaat tcctaccagt gactaatctt atgacttttt aaacagataa ctaaaattac
      121 aaacaaatcg tttaacttct gtatttgttt atagatgtaa tcacttcagg agtgataaca
      181 tgaacaaaaa tataaaatat tctcaaaact ttttaacgag tgaaaaagta ctcaaccaaa
      241 taataaaaca attgaattta aaagaaaccg ataccgttta cgaaattgga acaggtaaag
      301 ggcatttaac gacgaaactg gctaaaataa gtaaacaggt aacgtctatt gaattagaca
      361 gtcatctatt caacttatcg tcagaaaaat taaaactgaa tactcgtgtc actttaattc
      421 accaagatat tctacagttt caattcccta acaaacagag gtataaaatt gttgggaata
      481 ttccttacca tttaaacaca caaattatta aaaaagtggt ttttgaaagc cgtgcgtctg
      541 acatctatct gattgttgaa gaaggattct acaagcgtac cttggatatt caccgaacac
      601 tagggttgct cttgcacact caagtctcga ttcagcaatt gcttaagctg ccagcggaat
      661 gctttcatcc taaaccaaaa gtaaacagtg tcttaataaa acttacccgc cataccacag
      721 atgttccaga taaatattgg aagctatata cgtactttgt ttcaaaatgg gtcaatcgag
      781 aatatcgtca actgtttact aaaaatcagt ttcatcaagc aatgaaacac gccaaagtaa
      841 acaatttaag taccgttact tatgagcaag tattgtctat ttttaatagt tatctattat
      901 ttaacgggag gaaataattc tatgagtcgc ttttgtaaat ttggaaagtt acacgttact
      961 aaagggaatg tagataaatt attaggtata gggcacctct aataactacc aattaattca
     1021 cctcaaaatg aaattagaaa aaaacagcga aatttttaaa tctatttctt atcgatacaa
     1081 at
//
LOCUS       DACDIE010000270         1082 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.guided.200, whole genome shotgun sequence.
ACCESSION   DACDIE010000270 DACDIE010000000
VERSION     DACDIE010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.guided.200"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..55
                     /locus_tag="IV250_003035"
     CDS             <1..55
                     /locus_tag="IV250_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HAP8770224.1"
                     /translation="KTSTVLKQTKNSDYADK"
     gene            180..917
                     /gene="erm(B)"
                     /locus_tag="IV250_003036"
     CDS             180..917
                     /gene="erm(B)"
                     /locus_tag="IV250_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAP8770225.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          415 a          181 c          159 g          327 t
ORIGIN      
        1 taaaacatct actgttttga aacagactaa aaacagtgat tacgcagata aataaatacg
       61 ttagattaat tcctaccagt gactaatctt atgacttttt aaacagataa ctaaaattac
      121 aaacaaatcg tttaacttct gtatttgttt atagatgtaa tcacttcagg agtgataaca
      181 tgaacaaaaa tataaaatat tctcaaaact ttttaacgag tgaaaaagta ctcaaccaaa
      241 taataaaaca attgaattta aaagaaaccg ataccgttta cgaaattgga acaggtaaag
      301 ggcatttaac gacgaaactg gctaaaataa gtaaacaggt aacgtctatt gaattagaca
      361 gtcatctatt caacttatcg tcagaaaaat taaaactgaa tactcgtgtc actttaattc
      421 accaagatat tctacagttt caattcccta acaaacagag gtataaaatt gttgggaata
      481 ttccttacca tttaagcaca caaattatta aaaaagtggt ttttgaaagc cgtgcgtctg
      541 acatctatct gattgttgaa gaaggattct acaagcgtac cttggatatt caccgaacac
      601 tagggttgct cttgcacact caagtctcga ttcagcaatt gcttaagctg ccagcggaat
      661 gctttcatcc taaaccaaaa gtaaacagtg tcttaataaa acttacccgc cataccacag
      721 atgttccaga taaatattgg aagctatata cgtactttgt ttcaaaatgg gtcaatcgag
      781 aatatcgtca actgtttact aaaaatcagt ttcatcaagc aatgaaacac gccaaagtaa
      841 acaatttaag taccgttact tatgagcaag tattgtctat ttttaatagt tatctattat
      901 ttaacgggag gaaataattc tatgagtcgc ttttgtaaat ttggaaagtt acacgttact
      961 aaagggaatg tagataaatt attaggtata gggcacctct aataactacc aattaattca
     1021 cctcaaaatg aaattagaaa aaaacagcga aatttttaaa tctatttctt atcgatacaa
     1081 at
//
LOCUS       DACDIE010000271          988 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63401_2016
            SAMN12640251-rid9887993.guided.208, whole genome shotgun sequence.
ACCESSION   DACDIE010000271 DACDIE010000000
VERSION     DACDIE010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640251
            Sequence Read Archive: SRR10040965
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742909.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742909.1
            Genome Coverage       :: 60x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:24:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,909
            CDSs (with protein)               :: 2,909
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 26 of 66
            Pseudo Genes (incomplete)         :: 32 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 7 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640251-rid9887993.guided.208"
                     /isolate="E63401_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..348
                     /gene="thyA"
                     /locus_tag="IV250_003037"
     CDS             <1..348
                     /gene="thyA"
                     /locus_tag="IV250_003037"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP8770226.1"
                     /translation="FQFYVQEGRLSCQLYQRSGDVFLGVPFNIASYALLTHLIAHETG
                     LAVGEFVHTLGDAHLYINHIEQMKEQLSREIRAFPTLVLNQEKQSVFDFEMEDIQLEG
                     YDPHPAIKAPIAV"
     gene            368..895
                     /locus_tag="IV250_003038"
     CDS             368..895
                     /locus_tag="IV250_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP8770227.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
BASE COUNT          313 a          171 c          224 g          280 t
ORIGIN      
        1 tttcaatttt atgttcaaga gggacggcta agctgccaat tgtatcaacg cagtggggat
       61 gtgtttctag gcgttccgtt caacattgca agttatgcgt tgctgactca cctaattgca
      121 catgaaacag gtttagcggt tggcgaattt gttcacacat taggagatgc tcatctttat
      181 atcaatcata tcgaacaaat gaaagaacaa ttgagccgag aaatacgcgc atttccgact
      241 ttagttttga atcaggaaaa gcagtcagta tttgattttg agatggaaga tatccaatta
      301 gaaggatatg acccacatcc ggcgatcaaa gctcctatcg ctgtttagaa tcagaaagga
      361 tgagaaaatg ttagctgcaa tttgggcaca ggatgaaaac ggattgattg gaaaagaaga
      421 ccgtcttcct tggcgtttgc caaatgatct gaaattcttc aaacaaatga cagaagcaaa
      481 tacactagtg atgggacgaa aaacattcga agggatgggt agccgtccgt tgccgaatcg
      541 tcagacgatt gttttgacga gagattcttc ctataaagca gaaggcgtgc atgtcatgca
      601 tagtgtggaa gacgtgcttg ctctggaaaa agaaacagac ggtatctttt tcatcgctgg
      661 agggtcagct gtttatcaag aatttctgcc attttgtacg atattatacc gtacagtcat
      721 ccatcatgta ttcgatggag atgcttattt tccacctgtt gactggtctg attggtcact
      781 gatcaatact agtcaaggag aaatggatga aaaaaatcgt tatccgcatc aatttgaaac
      841 gtatcagcgt aatgagaatc ctgtaaaaag cgggacaggc gaattgacga tataaaaaag
      901 ctatggatag actagctgtt atttagagct gtcccatcca tagctttatt ttttttatta
      961 agtaaagaat aaaacagaga agtacata
//
